LOCUS       DAHUVV010000001       109012 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.001, whole genome shotgun sequence.
ACCESSION   DAHUVV010000001 DAHUVV010000000
VERSION     DAHUVV010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 109012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 109012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..109012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.001"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..1026
                     /locus_tag="NK767_000001"
     CDS             <1..1026
                     /locus_tag="NK767_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCH6693028.1"
                     /translation="LLGDHLTSIGDFFVLKSSVIEPGDSLQYFHIVLSAFLSALLAFC
                     LKKSLETWFHTVDRRMIGTIGTLILFTYYIIIFYTRFSGETPDVLALNTSFFILYLCV
                     GLIILVYYWKISNKKLADQLLEQQVRMQQDYIKDLEKNYQDLREFKHDYQNLLFSLHS
                     YISEGDLEGLRSYYDQTILPTRQMMNLFPANLSMLDNMKVPEIRSLLSLKLMMAQEKG
                     LIVQLHFPEPIELDTKHTVNLVRMLGIILDNAIEGASAAKKKKIELTILKKNHSVMIR
                     VINTTTNTLPLNQLKQKGFSTKTNPKNEGLGLFILDELTKTNPYLFLETSIENQRFSQ
                     TIYIQIK"
     gene            1039..1767
                     /locus_tag="NK767_000002"
     CDS             1039..1767
                     /locus_tag="NK767_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6693029.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(1836..3359)
                     /locus_tag="NK767_000003"
     CDS             complement(1836..3359)
                     /locus_tag="NK767_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCH6693030.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            3611..4792
                     /locus_tag="NK767_000004"
     CDS             3611..4792
                     /locus_tag="NK767_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCH6693031.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(4852..6480)
                     /gene="treC"
                     /locus_tag="NK767_000005"
     CDS             complement(4852..6480)
                     /gene="treC"
                     /locus_tag="NK767_000005"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCH6693032.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(6931..9642)
                     /locus_tag="NK767_000006"
     CDS             complement(6931..9642)
                     /locus_tag="NK767_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6693033.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(9652..10353)
                     /locus_tag="NK767_000007"
     CDS             complement(9652..10353)
                     /locus_tag="NK767_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6693034.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            10539..11138
                     /locus_tag="NK767_000008"
     CDS             10539..11138
                     /locus_tag="NK767_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCH6693035.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            11219..12232
                     /locus_tag="NK767_000009"
     CDS             11219..12232
                     /locus_tag="NK767_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCH6693036.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            12340..13257
                     /gene="rbsK"
                     /locus_tag="NK767_000010"
     CDS             12340..13257
                     /gene="rbsK"
                     /locus_tag="NK767_000010"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCH6693037.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            13285..13680
                     /gene="rbsD"
                     /locus_tag="NK767_000011"
     CDS             13285..13680
                     /gene="rbsD"
                     /locus_tag="NK767_000011"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCH6693038.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            13680..15053
                     /locus_tag="NK767_000012"
     CDS             13680..15053
                     /locus_tag="NK767_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCH6693039.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            15410..17554
                     /locus_tag="NK767_000013"
     CDS             15410..17554
                     /locus_tag="NK767_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCH6693040.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGNPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(17606..18646)
                     /locus_tag="NK767_000014"
     CDS             complement(17606..18646)
                     /locus_tag="NK767_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCH6693041.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18923..20725)
                     /locus_tag="NK767_000015"
     CDS             complement(18923..20725)
                     /locus_tag="NK767_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6693042.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(20739..21491)
                     /locus_tag="NK767_000016"
     CDS             complement(20739..21491)
                     /locus_tag="NK767_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6693043.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(21500..21862)
                     /locus_tag="NK767_000017"
     CDS             complement(21500..21862)
                     /locus_tag="NK767_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCH6693044.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(22038..22910)
                     /locus_tag="NK767_000018"
     CDS             complement(22038..22910)
                     /locus_tag="NK767_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCH6693045.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(22961..23449)
                     /locus_tag="NK767_000019"
     CDS             complement(22961..23449)
                     /locus_tag="NK767_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCH6693046.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(23587..24378)
                     /locus_tag="NK767_000020"
     CDS             complement(23587..24378)
                     /locus_tag="NK767_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCH6693047.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(24433..25041)
                     /locus_tag="NK767_000021"
     CDS             complement(24433..25041)
                     /locus_tag="NK767_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCH6693048.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(25034..25351)
                     /locus_tag="NK767_000022"
     CDS             complement(25034..25351)
                     /locus_tag="NK767_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCH6693049.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(25507..26235)
                     /locus_tag="NK767_000023"
     CDS             complement(25507..26235)
                     /locus_tag="NK767_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCH6693050.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            26394..27275
                     /locus_tag="NK767_000024"
     CDS             26394..27275
                     /locus_tag="NK767_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCH6693051.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(27512..28555)
                     /locus_tag="NK767_000025"
     CDS             complement(27512..28555)
                     /locus_tag="NK767_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCH6693052.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(28558..29226)
                     /locus_tag="NK767_000026"
     CDS             complement(28558..29226)
                     /locus_tag="NK767_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6693053.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            29368..31704
                     /locus_tag="NK767_000027"
     CDS             29368..31704
                     /locus_tag="NK767_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6693054.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(31819..32130)
                     /locus_tag="NK767_000028"
     CDS             complement(31819..32130)
                     /locus_tag="NK767_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693055.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            32311..33786
                     /locus_tag="NK767_000029"
     CDS             32311..33786
                     /locus_tag="NK767_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HCH6693056.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            33833..34417
                     /locus_tag="NK767_000030"
     CDS             33833..34417
                     /locus_tag="NK767_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCH6693057.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            34419..34838
                     /locus_tag="NK767_000031"
     CDS             34419..34838
                     /locus_tag="NK767_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HCH6693058.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(35119..35403)
                     /locus_tag="NK767_000032"
     CDS             complement(35119..35403)
                     /locus_tag="NK767_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HCH6693059.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(35755..36465)
                     /locus_tag="NK767_000033"
     CDS             complement(35755..36465)
                     /locus_tag="NK767_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HCH6693060.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(36462..37934)
                     /locus_tag="NK767_000034"
     CDS             complement(36462..37934)
                     /locus_tag="NK767_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCH6693061.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            38072..38545
                     /locus_tag="NK767_000035"
     CDS             38072..38545
                     /locus_tag="NK767_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCH6693062.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(38698..39249)
                     /locus_tag="NK767_000036"
     CDS             complement(38698..39249)
                     /locus_tag="NK767_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCH6693063.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            39354..39884
                     /locus_tag="NK767_000037"
     CDS             39354..39884
                     /locus_tag="NK767_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HCH6693064.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(40367..40810)
                     /locus_tag="NK767_000038"
     CDS             complement(40367..40810)
                     /locus_tag="NK767_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6693065.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(40826..41986)
                     /locus_tag="NK767_000039"
     CDS             complement(40826..41986)
                     /locus_tag="NK767_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCH6693066.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            42493..44193
                     /locus_tag="NK767_000040"
     CDS             42493..44193
                     /locus_tag="NK767_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693067.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(44295..45128)
                     /locus_tag="NK767_000041"
     CDS             complement(44295..45128)
                     /locus_tag="NK767_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCH6693068.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(45432..47597)
                     /locus_tag="NK767_000042"
     CDS             complement(45432..47597)
                     /locus_tag="NK767_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCH6693069.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            47857..50151
                     /locus_tag="NK767_000043"
     CDS             47857..50151
                     /locus_tag="NK767_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCH6693070.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            50144..50809
                     /gene="pgmB"
                     /locus_tag="NK767_000044"
     CDS             50144..50809
                     /gene="pgmB"
                     /locus_tag="NK767_000044"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCH6693071.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            50802..51809
                     /locus_tag="NK767_000045"
     CDS             50802..51809
                     /locus_tag="NK767_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCH6693072.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            51831..52850
                     /locus_tag="NK767_000046"
     CDS             51831..52850
                     /locus_tag="NK767_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6693073.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(53131..54498)
                     /gene="nhaC"
                     /locus_tag="NK767_000047"
     CDS             complement(53131..54498)
                     /gene="nhaC"
                     /locus_tag="NK767_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCH6693074.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(54607..56028)
                     /locus_tag="NK767_000048"
     CDS             complement(54607..56028)
                     /locus_tag="NK767_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6693075.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(56271..58148)
                     /gene="tdc"
                     /locus_tag="NK767_000049"
     CDS             complement(56271..58148)
                     /gene="tdc"
                     /locus_tag="NK767_000049"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCH6693076.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(58439..59695)
                     /locus_tag="NK767_000050"
     CDS             complement(58439..59695)
                     /locus_tag="NK767_000050"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCH6693077.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVGALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(59807..60012)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            60275..61576
                     /locus_tag="NK767_000051"
     CDS             60275..61576
                     /locus_tag="NK767_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCH6693078.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(61645..62244)
                     /locus_tag="NK767_000052"
     CDS             complement(61645..62244)
                     /locus_tag="NK767_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCH6693079.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            62498..63379
                     /locus_tag="NK767_000053"
     CDS             62498..63379
                     /locus_tag="NK767_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6693080.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            63392..65002
                     /locus_tag="NK767_000054"
     CDS             63392..65002
                     /locus_tag="NK767_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HCH6693081.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(65079..65738)
                     /locus_tag="NK767_000055"
     CDS             complement(65079..65738)
                     /locus_tag="NK767_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCH6693082.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(66021..66686)
                     /locus_tag="NK767_000056"
     CDS             complement(66021..66686)
                     /locus_tag="NK767_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HCH6693083.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(66833..67438)
                     /gene="dhaL"
                     /locus_tag="NK767_000057"
     CDS             complement(66833..67438)
                     /gene="dhaL"
                     /locus_tag="NK767_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCH6693084.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(67466..68476)
                     /gene="dhaK"
                     /locus_tag="NK767_000058"
     CDS             complement(67466..68476)
                     /gene="dhaK"
                     /locus_tag="NK767_000058"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCH6693085.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(68451..68897)
                     /gene="dhaM"
                     /locus_tag="NK767_000059"
     CDS             complement(68451..68897)
                     /gene="dhaM"
                     /locus_tag="NK767_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCH6693086.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            69000..69320
                     /locus_tag="NK767_000060"
     CDS             69000..69320
                     /locus_tag="NK767_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCH6693087.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(69432..70301)
                     /locus_tag="NK767_000061"
     CDS             complement(69432..70301)
                     /locus_tag="NK767_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCH6693088.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(70397..71701)
                     /locus_tag="NK767_000062"
     CDS             complement(70397..71701)
                     /locus_tag="NK767_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCH6693089.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(71698..72207)
                     /locus_tag="NK767_000063"
     CDS             complement(71698..72207)
                     /locus_tag="NK767_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693090.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(72382..74517)
                     /locus_tag="NK767_000064"
     CDS             complement(72382..74517)
                     /locus_tag="NK767_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCH6693091.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(74600..76255)
                     /locus_tag="NK767_000065"
     CDS             complement(74600..76255)
                     /locus_tag="NK767_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCH6693092.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(76257..76571)
                     /locus_tag="NK767_000066"
     CDS             complement(76257..76571)
                     /locus_tag="NK767_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCH6693093.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            76741..77001
                     /locus_tag="NK767_000067"
     CDS             76741..77001
                     /locus_tag="NK767_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HCH6693094.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            77015..77329
                     /locus_tag="NK767_000068"
     CDS             77015..77329
                     /locus_tag="NK767_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HCH6693095.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(78059..78613)
                     /locus_tag="NK767_000069"
     CDS             complement(78059..78613)
                     /locus_tag="NK767_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCH6693096.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(78739..80668)
                     /locus_tag="NK767_000070"
                     /pseudo
     CDS             complement(78739..80668)
                     /locus_tag="NK767_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(80974..81690)
                     /locus_tag="NK767_000071"
     CDS             complement(80974..81690)
                     /locus_tag="NK767_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCH6693097.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(81691..83514)
                     /gene="glpO"
                     /locus_tag="NK767_000072"
     CDS             complement(81691..83514)
                     /gene="glpO"
                     /locus_tag="NK767_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HCH6693098.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(83528..85024)
                     /gene="glpK"
                     /locus_tag="NK767_000073"
     CDS             complement(83528..85024)
                     /gene="glpK"
                     /locus_tag="NK767_000073"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCH6693099.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(85160..86620)
                     /locus_tag="NK767_000074"
     CDS             complement(85160..86620)
                     /locus_tag="NK767_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6693100.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(86610..87905)
                     /locus_tag="NK767_000075"
     CDS             complement(86610..87905)
                     /locus_tag="NK767_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCH6693101.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(88237..88914)
                     /locus_tag="NK767_000076"
     CDS             complement(88237..88914)
                     /locus_tag="NK767_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HCH6693102.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(88944..90035)
                     /locus_tag="NK767_000077"
     CDS             complement(88944..90035)
                     /locus_tag="NK767_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCH6693103.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(90136..90570)
                     /locus_tag="NK767_000078"
     CDS             complement(90136..90570)
                     /locus_tag="NK767_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCH6693104.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(91154..92611)
                     /gene="ascB"
                     /locus_tag="NK767_000079"
     CDS             complement(91154..92611)
                     /gene="ascB"
                     /locus_tag="NK767_000079"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCH6693105.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(92608..94503)
                     /locus_tag="NK767_000080"
     CDS             complement(92608..94503)
                     /locus_tag="NK767_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCH6693106.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(94561..96336)
                     /locus_tag="NK767_000081"
     CDS             complement(94561..96336)
                     /locus_tag="NK767_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCH6693107.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(96386..97882)
                     /locus_tag="NK767_000082"
     CDS             complement(96386..97882)
                     /locus_tag="NK767_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCH6693108.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(98076..99698)
                     /locus_tag="NK767_000083"
     CDS             complement(98076..99698)
                     /locus_tag="NK767_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCH6693109.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(100026..101339)
                     /locus_tag="NK767_000084"
     CDS             complement(100026..101339)
                     /locus_tag="NK767_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCH6693110.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(101584..101808)
                     /locus_tag="NK767_000085"
     CDS             complement(101584..101808)
                     /locus_tag="NK767_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693111.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(101828..102250)
                     /locus_tag="NK767_000086"
     CDS             complement(101828..102250)
                     /locus_tag="NK767_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCH6693112.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(102392..103102)
                     /locus_tag="NK767_000087"
     CDS             complement(102392..103102)
                     /locus_tag="NK767_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693113.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(103120..103617)
                     /locus_tag="NK767_000088"
     CDS             complement(103120..103617)
                     /locus_tag="NK767_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6693114.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(103654..104106)
                     /locus_tag="NK767_000089"
     CDS             complement(103654..104106)
                     /locus_tag="NK767_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCH6693115.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(104241..104846)
                     /locus_tag="NK767_000090"
     CDS             complement(104241..104846)
                     /locus_tag="NK767_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCH6693116.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(105296..105916)
                     /locus_tag="NK767_000091"
     CDS             complement(105296..105916)
                     /locus_tag="NK767_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCH6693117.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(105918..106787)
                     /locus_tag="NK767_000092"
     CDS             complement(105918..106787)
                     /locus_tag="NK767_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HCH6693118.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(106810..107217)
                     /locus_tag="NK767_000093"
     CDS             complement(106810..107217)
                     /locus_tag="NK767_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCH6693119.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(107236..108525)
                     /locus_tag="NK767_000094"
     CDS             complement(107236..108525)
                     /locus_tag="NK767_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6693120.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            108583..>109012
                     /locus_tag="NK767_000095"
     CDS             108583..>109012
                     /locus_tag="NK767_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCH6693121.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT        32052 a        22621 c        19148 g        35191 t
ORIGIN      
        1 cttttaggag atcacttaac aagcatcggc gatttttttg ttttgaaatc ttcggttatt
       61 gaaccaggag attcattgca gtattttcac attgtcttat ccgctttttt atctgcttta
      121 ttagcttttt gtttaaaaaa atcgttagag acttggtttc acactgtgga ccgccgtatg
      181 atcggtacga tcgggacatt gatattattt acatactata tcattatttt ttatacccgt
      241 ttttctggtg aaacacctga tgtcttagct ttgaatacga gttttttcat tctatatctg
      301 tgtgttggcc tgatcatctt ggtttattat tggaaaatct ctaataagaa attagctgat
      361 cagttattag agcaacaagt caggatgcaa caggattata tcaaagatct agaaaaaaac
      421 tatcaagact tgagagaatt caaacatgat taccaaaatc tgcttttttc tcttcacagc
      481 tatatttcag aaggcgactt agaaggatta cggtcttatt atgatcaaac gatattacct
      541 acacgccaga tgatgaacct ttttccggcc aatctaagta tgttggataa tatgaaagtt
      601 cctgagatcc gtagtttact gtcattaaaa ctcatgatgg cacaggaaaa agggttgatc
      661 gtccagcttc attttcctga gccaatcgaa ctggatacga aacatacggt aaaccttgta
      721 cgtatgcttg gtattatctt ggacaatgcg attgaaggag ctagtgctgc aaagaagaaa
      781 aaaatcgaat tgaccatact caagaagaat cattccgtga tgattcgtgt gatcaataca
      841 acaacaaata cattaccgtt aaaccagttg aaacaaaaag ggttctcaac aaaaacaaat
      901 ccaaaaaacg aaggccttgg cttgtttatc ttggatgaat tgacaaagac caatccttat
      961 cttttccttg aaacatcaat tgaaaaccag cgtttttctc aaacgattta tatacaaata
     1021 aaataaagga gtaatgatat gattcccatt tatatttgtg aagacgatcc aaaacaatta
     1081 gaagttttat caacggtgat caaaaataga atcatgattg aaaacttaga cagttatgtc
     1141 catatttcta cctctgatcc ggaagaactt ctcaatgcag ccaagatcag aacttcttct
     1201 tttggtattt actttttaga tatcgaacta gaagacagtg aaatggatgg tgtagcgatc
     1261 ggtaaattga tccgtgaatt agatccttta ggaaaaataa tctatgtgac ttcccatacg
     1321 gaaatgtcga tgaaaatctt aaaaagcaac atcgaaccga ctgattatat cgtcaaagaa
     1381 gatctttatg atttaaaaga aaatgtcgag catatcctga gcaaaatttt cgaagatttt
     1441 caaatgtccc aaatccaaaa agatatcttc aaaatagagt ttaatgatga gatcaaattt
     1501 ttaccgatca aagacatcca atatttttct acttcgctag gagcgccaca taaactggaa
     1561 gtccatttga ctcatgccca attacaattc tatgagaaga tcaaagaaat cgagaaaatg
     1621 catcggaatt ttatccgttg tcataaatcc tatgttatca atacacaaaa tgtgcggtcg
     1681 attaataaaa aaaccagaga agtcacgttg gcaaacggcg agaccatccc agcctctatc
     1741 cgtggattaa agaaattgat ttcataaaaa gaaccgaata gtttggaccg actaaatgac
     1801 cagccgatcc aaacccttcg gttctttttt gcctatcagt gcaaaagcat ttcctgtgca
     1861 atttcacgtc catccattgt atctggataa aatgttggcc aattctccat ttctttcagc
     1921 aatacttctt gactttcatt tcctgcatat aaatgaaaat gttcggagtc agccggtgca
     1981 atcgtgtgat cactgaattg aatatatcta aaagcgccac tttccttatc ttctgcttcg
     2041 aatagatacc ttacgccacg gtttcctgcc tcgtaggtca agatttcttt ccctacataa
     2101 cgataatttg aacgtttcgt tgttccattc ttaaacgtaa atgccatctc tttctcactg
     2161 atcttgattg aagcaatatc tgtttgatag cccttcgtat aatattcttt atattcttgt
     2221 gctgtcttat cctgattcaa tttttcttta tattcaaaaa caggatcaaa agtcccatct
     2281 tctacatacg tatctactgt ttgccaagtt cctgcccaat ctgaaagaga gcgatcttgt
     2341 acatcctcgt cttcaaaata accgttgtag acgctctttt cttttttggg ggtcaaagta
     2401 tcttctagcg ggttttctgt ctctgtcgtt tttttcaacg cttcgaggtt tgcttccatg
     2461 atagaaagat agttttctcc attttctatt tgttcattcg tcaatccttc caaaggattc
     2521 aatacttcta aagatacacc tgcttcgttt gctaacgttc tggcaatact atctttggcg
     2581 ttttcttcaa aataaatata gttgattcca tgttctttaa caaagtgttt cagttcagca
     2641 attctcgccg cagacggttc ttcactagaa gaaagaccag caatcgatac ttgattcaat
     2701 ccatattcta acgctagata gttgaatgca gcatgttgcg tgacaaaaga tctttgtttt
     2761 gcttgcttaa aggtttcttg ataaagctga tggagtgctt gaagtttctc ggtataggct
     2821 gctgcatttt ttgtccatac ttcctgtttt tctgggtaag cttcaatcag ctgatcacgt
     2881 atgtttgcta cctgcttgat tgctaaactt ggagctagcc atacatgtgg gtctagctca
     2941 tgtgtatggc cttcttcccc gtgttcatgg tcatgatcat gtccttcttc acttcccgga
     3001 agtaaaagca taccttctgt cgcttttatc gtatggacat tgccttcttg taatgttttt
     3061 tggattgcgg ggacccatgt ttccatgttt tcatcattat aaacaaagat atctgtatct
     3121 tggatctttg ccatgtcttt agcagaaggt tcataatcgt gggaatcagt ccctgctgga
     3181 atcaataact ctacttctcc ttcatccccg acgattgctt ttgtaaaatc atacatggga
     3241 taaaaggtcg tcatcacgct caatttttct ttactgtcct cggctttccc acttgccccg
     3301 caagcagtta acatacctgc taaaaccagc atggccaata tacctatcaa tcgtttcatt
     3361 tatgacactc ctcttctcat cttctttcat ttgaatgttc cgattcgctt ttcgcaaacc
     3421 gtaatagtta cgttttagca acaggattta atttatcata tggttaaaag aactgtcaag
     3481 cgttttctta ttttaattga tgagaaagtt tatatgaatg attgacgata tttttgcaat
     3541 caacctctat cattttcctt ttatacccgc ttttgtctat aatgaattta tagataaagg
     3601 aggaaaaaga gtgaaaaaag aaacagatac aacgaatcgt ttgatccgct ttttaggagg
     3661 aaaaacctct tactatgttt taggaatagt tatcttactt gcgctgatca tttatttttt
     3721 ccatcagatc tcgtttatat tcaagcctgt cggaatcatt atcgctacta tcttgccccc
     3781 attattgttt gcactgatct tgtattatct tttggaacca gtcatcaaac gtttgagcaa
     3841 gcacctttcg aggaacttgt cagtcattat cgtttatgtg attattttat ttttattggg
     3901 gcttggaagc atatggctta ttccttttct tgaagaacag gcaaagacat tgatcagtag
     3961 tttgccagat ttgtttcaag attttcaaga gagtctccga caatttttgg agaaaacacc
     4021 ttttgccgat tcatttaatc aatttttcgc ttctattgac gacgtgacgt ctgatatagc
     4081 tggatttatt ggaaattatt gggaaagtgg cgcacaacgg atcagcaaca ttttttcaac
     4141 tgttacagct atttttatta ctttatttac tggtccaatt gtcgcttttt tccttttgag
     4201 agatcctcaa aaattttatc agtcagtttt agcgattgtc ccaccagcat ttcgtacaga
     4261 ttttaaaaat ttgacaaaga ttgctaatca acaattgggt tcatttctaa aggggcaaat
     4321 cattgcttca tttatccttg gagcagtcta ctgggtttgt ttcttactga tcggattaga
     4381 atttgctagt gttttagcta tttctgctgg gttgttatgt atcattccat atattgggcc
     4441 gtttatcgcc ttctttccag gtttgatcat tgcattccaa gactctactt ttatggctgt
     4501 caaatttgtc attgtctggt ttgccgtaca acttctccac ggtgacttgg tgattcctcg
     4561 agtaatgggt gatcgtttac agatccatcc gattacgatc ttaatcgttt tacttgtcat
     4621 gggtgatcta atgggaattg tgggcgttat ttttggtatt cctatctata cgttagtcaa
     4681 attactcgtt acttttattt tccgtaaatt caaacaacgc tacaataaat tttatggcga
     4741 taaaggagag tatgaaaact cttcattttc tgaagaagat tattttaaat aaaacacaaa
     4801 acgtcgatct tacgagaata gctcgtagga acgacgtttt gtgtttttgt tttaattttt
     4861 cagcaaagca attgcctggt atggttttag ctccagactt tgatgaagaa cagtatcgga
     4921 ataattatcg ataagaatat tggcttctgt gaatccttct gggatggcga tccttgtatc
     4981 tttatcatag aaatttgtta atacaagcag ctgctgtcct tcaaattggc gaagaaagcc
     5041 ataaacttcc ggatgatttg cttcataagc atgataatct ccttgtgcaa cgattggata
     5101 tgtttttctc agccgaatca atttcttgta ataggacaaa atagaatctt cagaagatag
     5161 ttctttttct acattaatct cttctagctt gcctccaact tttaaccaag gtttccctgt
     5221 agtaaatcct gcttgttctg tgtctgacca ttgcatgggt gttcgagagt tatcgcgaga
     5281 ttttgctttg atccgacgaa acgcttcttt ttctgtgaat ccttgttcaa ggagtaactt
     5341 ataagcattc aaactttcta cgtcaatata gtcggaaatc gatgaatagt ctgggtcgat
     5401 catgccgatt tcttctccca tataaatata gggcgtccca cggttcaaat ggatcatggt
     5461 tgctagcatc tctgctcctt gttttcgata ctgatcgata gctacaaatc gattgagcgc
     5521 acgtggctga tcatgattat taaaaaatag agcattccag ccgttaccct cactcatctt
     5581 ttctccccat gtatgaaata gtcgctttaa ttctgcaaaa tcaaatggag gagtagacca
     5641 tttattccca ttttcataat cgactttcaa atgatggaaa ttaaagacca ttgataattc
     5701 ttctctacta ggttctgtat acaagataca attttcgatc gtcgtagagc tcatttcacc
     5761 gactgtgatg atagtggaat ccgtcccaaa cgttgcttgg ttcaattctt ttagatacgt
     5821 atgggtgatt ggtttgtccg tatattcagg tttgccatca ttttctttat tatttttcaa
     5881 ttgttcgtct ttaccgataa cattgataac atcgaaacgg aatcctttta cccctttttc
     5941 catccaaaaa cgaacaactt tgaatagttc ctctcttact gcttggttcc gccagttcaa
     6001 gtcagcttgt gtccgatcat ataagtgcaa ataatatttc cccgtatctc caaatggcga
     6061 ccaagcactt ccaccaaatt ttgataccca atccgttggc tcttctctta aaatgaagaa
     6121 gtcctgataa tgctggtccc ctgctagtgc ttttttgaac cattcatgtt ctgtcgaaac
     6181 gtgattcagc accatatcga gcatgatttc tatccggtat ttctttgctt cggctattag
     6241 ctgttcgaat tcctccatcg taccaaagat aggatcgatg gctgtgtaat cagagatatc
     6301 ataaccgttg tctttttgag ggcttggata aatagggttt agccaaatca tgtcgatgcc
     6361 tagatcattc agatacggga gtttttcttt gattcctttt aaatctccaa ttccatcacc
     6421 attactgtct ttgtaagatt tgggatagat ttggtaaatg actttgtctt taaatgtcat
     6481 ggatatttac ctccattcaa ctgatttatt acctaccaca acttatatat acaagttatc
     6541 atttattatt ataactgaac cgaaagcgtt tgtaaaattt atctttatac attctaagag
     6601 gtctgttgcc caataaaaaa caagagatca gacatgtatt tgtctatcct cttgtttaaa
     6661 atagcaaaat attcttgttt cttctataaa tggtgttcaa aagcttttat ctacggcaat
     6721 cagtcttctc aaatgtaaaa catgaggagc taattttatt tgagtattct ttttgctcga
     6781 tgcaggacgg cttttttaca acctctgatt catggtgttc aatcagctgt ccttcggtat
     6841 tagctcaaaa aacggcaaaa tatgaaaaac tattttgccg tttgtttctc taatactcag
     6901 gtcaaaccgc tgatttcaca acctctgttt ttattcattc gatttcaacg catcaatcat
     6961 atcgatatgc tttaatttga aatgggtaac aatcatcaca ataaccgtaa atacgatagt
     7021 taatcctgct gataagacat aagcccacca tgtgatcact aatggaaaga tgacattctc
     7081 catagacgct tgttgcaaga tgaagtcagt caatatatat cctacaccaa atcctccgat
     7141 gattcctaaa agagtaaaga tgatattttc tcgaacaatg tacatcgtga cttctttatc
     7201 aaaaaatcca aggactttta ttgtagataa ttcgcgtaca cgctcggaaa tattgatatt
     7261 agttagatta tacaagacga taaaagccag cagtcctgat aatacgacaa agattaagac
     7321 aaccgagtct aagttcgcca ttgattcttc ttgcgtttcg atttgtttgg acatgaaaga
     7381 cgtatttgtc acagcgtctg tggcaagcag tttctcggaa agcatttctt cttcttttgt
     7441 tgtcatctta tctgtacgca gcaaaaatgc attatccatc atcgattgat cagtaactga
     7501 agcataatat tcaggggaca tataaataaa gctacctaga tagtttttca cgatttcttt
     7561 tacttcgact ttccattctt tttgatcttc atcataaagc gaaatatgat ctcctgtaga
     7621 taagtcaaat agttctgcta ctttttgcgt aatagcgatt cctgttttag gcaaggagcg
     7681 tgggttctct tcaagatcag ttaaatggat aaaacgatcc aaatcagctg ttttacttgg
     7741 tacgatcact gtcagacttt gcgaagcatg tccttttttt ctcaattcta cggtttgagt
     7801 atgaatagct aggatctcat ttactttttc ttgcttctgc agcatatccg ctgctttttg
     7861 acgactatct tccgtggatg aatccaatgt aacgattgct tgatagtcaa tgatcgttcc
     7921 aaattgtttg gctgaaacag aacttagaga atctttcaat cccacgcctg caaccatcag
     7981 cccggcacat ccagcaattc cgataatcgc cataagcatt cttgacttat atcggaagag
     8041 atttcggtaa ctgatttttt gattaaagct aaggcgtgac cacagcggag tgatgtattc
     8101 aagaaaaatg cgtttccctg gcttcggtgc tttcggttga agcaatgcag caggccgttc
     8161 tcttaaatct ttgaataaaa cgatcattgc agcgccaaat gcggcaatga agaatgcggc
     8221 agctgcttgt aaaatcggcg gccagtcata aaaaataagc gcactcccaa tatcataccg
     8281 ctcgttagac agctcaaaaa tgatccttgg caaaagattc gttcctaata tcgttcctaa
     8341 aacaatacct atccctgagg ctagacttgc atagattgca tatttttttg cgatttcaac
     8401 tttcgtataa cctaacgcct ttaatgtccc gatttccctg cggttttctt ctaccatacg
     8461 tgtcattgtc gtaaaggtga tcaatgcggc aataaagaaa aagaaaacag gaaaaacatt
     8521 ggcaatcgca gcgatacgat cagacagatc accaaattct tgaaaaccga cattttctga
     8581 acgttcattg tataggtatg atggtttagt taaattttca attttttctg catttcgatt
     8641 caattcttcc tctgcagagg ccagttcacg ttctttctca actaattgcc tttcttgttc
     8701 agcaagttga gcttcttgtt gagataatgt ttgaacagcg attgttcgtt gcgcttcagg
     8761 aagctgctgg atagttgctg actgttgcgc taactgtttt ttagcatctt ctaaccgtgt
     8821 tcttgcttgc gttgtttgtg ctttgccatc cgctattttt tgctgtgccg gttctaattc
     8881 ttcgttggcc gattttttca gctcttctaa gcgttcttcg ggtcgatctt gtaaatactt
     8941 ttctgccttt tcttgatttt gctccatttt atctttgtaa gctttgctgt acgtattcaa
     9001 attctttacg tttgtaaaat caagatataa gacagaataa atatctgttt taaaaacgga
     9061 ttcaggtaaa tagacaaaat aatcgacgct accatttccg acgttcgcgt atcctctctc
     9121 caaattgttg atgaacaatg gagaacggac aaagcccgtg atggtatact ctttttcttt
     9181 caattcatct gaatcaatct gataaatatc accgatttta tacccttcat cttccgcttt
     9241 ttcatccaat acaagctggt ttggtttttc cgggagtttt ccatcagtta attgtagctt
     9301 gttctgcttg ttatttttgt tataagaaaa tatttgaatc acttcgtttt tgccagtatt
     9361 ggaataatag atctgttttc ctgtttctac ttttgcatct gggatctttt ctgcttgagc
     9421 aatatctttt tgtgtcaagc caccagtaga aacgatttgc aaatcagaaa gtcctgctgt
     9481 accaacatag tgatccatcg ttttttgcat gacaggtcca gcagttttga cacctacaaa
     9541 taaaagtgtt cctagcatga caataaggat gattgcaatg aatcgtcctt tagattgccg
     9601 aatttcgcga aaacctgctt taagatatgt tcttgttttc ataaaatccc cctaccatac
     9661 aatctcatca atggatgaag gatgatcatt taattcaaca gaccttactt tagcatcatt
     9721 aatgtgaatg actctatcag cgatgggcgc taaagcagaa ttatgtgtaa tgatcaaaac
     9781 ggtattgccg tgttgtctgc tagcgttttg caataatttc aatacttgct tgcccgtttc
     9841 aaaatccagc gcgcctgtcg gttcatcaca taaaagaagt ttgggatttt tagctagtgc
     9901 acgagcaatt gaaaccctct gctgttcgcc tcctgataat tgggacggaa agttattcaa
     9961 tcgatgagct aggcccactt gcctcaatac ctctacagga tcaagcgcat caggagaaac
    10021 ttccgttgcc aattccacat tttcttttgc tgttagatta ggaacgagat tgtagaattg
    10081 gaaaacgaac cctacatctg ttcgtcggta agcagtaagc tgtttatctg aaaattgcgc
    10141 aatatctgtg tcatcaataa tgatctgacc ttcatcaggt gtatccatcc cacctaaaat
    10201 attaagaatc gttgactttc ctgctccact tggaccaagt ataatcacta attcgccttt
    10261 ttcaattgaa aaagaaatat cgtcatttgc tgtaatggtc gtttctccca ttttgtatcg
    10321 tttgtattca ttttttactt cgacatactt cactctgatt cctccatttt cacgatattt
    10381 ctcgctatat ctatgcatcg aatgcgtttt ctcacactta tttattttac cactgaattt
    10441 ctctagacta aagaaaggaa tcctttaatt tagttgacaa ataaacgatt tatgatttac
    10501 ttacataaaa aacattgtta tttgaaagaa ggaacatcat gaatgattta aaggaaaata
    10561 tgatttttga tttatcaaaa actgcttttg tcgcgatcga catgcaaaaa gagatcgtaa
    10621 ataacggtac actctctcca cacactgcag catctattct gacaaacaat gatttgttag
    10681 taagaacatt gaaacgtacc gctgccttga aagttttagt gaacgtagac atctccactt
    10741 tcccttattt gagtcaacaa actgatatgg gtggaatgac tgcttctgta cctccagaat
    10801 tcactgattt acttttgaag gattctttga aagatacaga taatatgttg actatcacaa
    10861 aatacaatcc aagtgctttt tttggtactt ctcttgatct tcaacttcgc cggcgtggaa
    10921 tcgaaactat cattttaagc ggtgtagcga caacaaacgg tgtatatgct actgcattag
    10981 atgcctttca acacgggtac catatcgtgc ttgctgaaga tgcttgcagt gatcgtgaca
    11041 aagaatccca tcaattgttc ataaaaaaga tatttccgaa aactgcacga gtacgctcaa
    11101 cgaaacaaat aatcgaagct atccaacaaa gcaaataagt actggcacac gtgattttca
    11161 tttacttctt tttgatttca tgggaaaata accacaggat ttagatagga gtatgaacat
    11221 ggtgaaaaaa aagatcacaa tcaaagaagt cgctaaactt tccggcgttt caatcacaac
    11281 tgtctccttg atattaaatg gaaatacgga gaaattcagt ccgaaaactg ttcagaaagt
    11341 tttagctgcc aaagaagaat taggctatca gcctgactat tttgctagac aaatgattac
    11401 caaggaaacg aaaacaattg gtgtactcgt tcctgatatc acgaatccat tcttcagtac
    11461 actgatgcga ggaattgagg atatcttgta taagcagaat ttcgtcacga tattatgtaa
    11521 tgctgattct gatcatcaaa aagaaatcga atatctagcc gagctcacac gtcgaggtgt
    11581 ggatggattc attatcgcga cttctgctgt ctctacagat gcaatcaacg aaaatttgaa
    11641 aaaacaaggc cggcccttca ttgttttgga tcagaaaaaa tctgaaggat ttagtgatgc
    11701 cgtacgaaca gacgattttc gcggtggtta cctagcgggc atgcacttgc ttagtctagg
    11761 acatcaaacg atcgcccttg tctatcctga aaatccaccg gaaaatgtcc acgctcggat
    11821 tgaggggttt aaatctgctc ttgatgttta ccaaatccct cacgaccaac tgatcctctt
    11881 acctacacag ttctcaaaac aaggtggcta ccagatcaca gcagaacttc ttgattctgc
    11941 tgcaactggt gtgtttgctt taaatgatga attagctttt ggattatatc gcggattaga
    12001 ggaagctgga aaaagcatcc ctgaagacta cagcattatt ggctatgata atatagatat
    12061 gtgcgagtat atcaagccga aactgacaac gatcgcacaa ccaatcttcg aattgggaca
    12121 aacatctgcc aagttattgt tagatcggat ccaattccca gaaaaagaat gggaagaaaa
    12181 aagattacct gtacgattag aaaaaagatt ttcaactgca cccttgaaat agctttcaag
    12241 gtgtgctata tttttatcga actattaaaa cgttttacta aaacgtttta aaaaataatt
    12301 ttcccatcaa aataaatgag atagttggag gtttgtaaaa tgaacacggt tacagtaatt
    12361 ggaagtatta atatggacac tacccttcgt ttaaccaaca tgcccaagcc aggcgaaacg
    12421 atgcatgcgc atgagatttt tcacgctggc ggaggtaaag gtgccaacca agcagtggca
    12481 gccaaacgtt caggcgcacg gacatctttt attggaggcg ttggggctga cagcgaagga
    12541 caacagttat tagatctact gacaaaagaa aacatcgata catctgggat agctgaaatc
    12601 caaggcgcca caaccggaca agcaatgatt atggtagacg cagcaggaga aaatagtatc
    12661 ttgatccatg caggggcaaa taatgctttc catgaacaag aagtcctaaa aaacaagcaa
    12721 ctgattacaa acagtgactt tattattgct cagtttgaaa gtagcttaga tgcgacgatc
    12781 ctagcttttt ctattgcaaa agacgcagga aaaacaacga tcttgaatcc tgcaccagct
    12841 cgtgaaacga ttcctactga actacttgaa aagacagata tcattatacc aaatgaaaca
    12901 gaaacagaaa tcattacagg aatcagagtg acagatcata acagtttggt cgcagcagcc
    12961 gaaaaactcc atgaattagg tattggaaca gtaatcatta cactaggcag tgccggcgct
    13021 ttctatcata cagaaaaaga acacggtatc gtccctgctt ttaaagtgga tgccgttgac
    13081 acaacagccg caggtgatac atttattggc gcattaagca gcacacttca gccagacctt
    13141 agtaacttga aagaagcgat cctttatgga aatctagcct cttctgttgc tgtgcagtct
    13201 tatggtgcgc aaccttctat cccttaccgc gaagctttag aacaatcagc agtctaaaat
    13261 taacaatgag aggaagaaac aaacatgaaa aaaggaaaag taatcaattc agatatttca
    13321 cgagtaatcg cacaaatggg gcactttgat aaattaagta tcggggatgc aggcatgcct
    13381 gtcccctttg gtactgaaaa aatagatttg gctgtctcaa acggtcttcc aagtttttta
    13441 tctgttttag aaaacgtttt agaagaactt gaggtacaat cgattgaatt ggcggaagaa
    13501 ataaagacaa tgaacccgga aatattggaa aaaatcaaat tattgttacc agatacaccg
    13561 attagcttta tcccacatga agaaatgaaa aaagagttga ataactgtca tgcatttatt
    13621 cgtacaggcg aaatgacacc ttacgccaat attatattag tgagtggagt ggtcttctaa
    13681 tggaaacgac aatgaatcct gaacagatgg tactgtccga gcagattcat atgctgattg
    13741 aacagttggg aggaaaagac aatattgatt ctgtccttcc atgtgcgact cggcttcgta
    13801 tcaaattgaa aaaacagcca gtagccaatg cagaaaaatt aaaacagcaa atcccttcca
    13861 ttaagtattt cattttactc gatagatatg cagaaataat catggatcag ggattggctc
    13921 ctgcttacaa ggaattgacg cgtcaaatcg atcattcagc tccgaaagaa aagctaaatc
    13981 cgatcgaaaa actatttggt ttgatatcgg aaatctttac gccgatcatt ccgctgtttg
    14041 ccggttctgg tatattaaaa ggtctggttg ttttagcgac tacgattggc tggcttagtg
    14101 aaacaagcgg aacttatcat attttgtctg ctgcgtcgaa tgccgtcttt tattttctgc
    14161 caatctttct tgctttttct gctgccaaaa cgtttaaagt gaatggatat attgcggctg
    14221 tcattgctgc agcactgatt gaaccgaatc tgacctcttt actttcagac agtggcaaag
    14281 cagctactga ttttctgggg attcctgtag tactgatgca gtatacgtct actgtcatgc
    14341 cggcgatttt aggtattttc ttttattctt ttgttgaaaa attcctcaaa aaatatattc
    14401 gcgaaaatat gcaattagta tttgtacctt tactttcatt agttatcacg gttcctttga
    14461 cgttaatggt attcgggcct gcgggtgttt accttggcga aggtttagct gccgcaatta
    14521 cttggatgat ggatattaac ggaccgatca gtggcgcaat catcgctggt ggttggaata
    14581 ttttggtaat atttggtatc caatgggctg taaaccctgt catgatcagt aatatctctg
    14641 cttatgggtt cgaccgaatc gttccgttga caggagcagc caattttgga atggctggtg
    14701 cagctttagg tgtatttctg cgaagtaaac gttcaaaaac ccgttcgatc tccggttctg
    14761 cttttgcttc gattttactt gcgggagtga ccgaaccaac tgtttacgga attgcgatcc
    14821 cattgaaaaa accatttgtt gctgcttgca ttggtgcagc tgctggtgga gctgtaatgg
    14881 gatttgccca agtgaaagcg atcgcttttg tatttggtag tctgaccaca ttgcctgcat
    14941 tcatttccgg tacttttttc tggtacttag caggactggc tgttagtcta gtcgtagcaa
    15001 tgatcactac tctcgttagc gggtttgatg aagacctaat gtcttatgaa tagttaatga
    15061 aaaagactga agtatgagat gtttatcaca tatttcagtc tttttctttt taggatcatt
    15121 atttttataa atttttgtga tactttccaa actttggtat aatttacata tgcataacca
    15181 tttttatccc cacttgttta tccccaaaca aaggtggtct atgcaataaa atgaacgtca
    15241 aagaaaaacg aatgaatgct cgttgcttat ttacaaattg ttttatttag aatacacaga
    15301 cagatttatg aaaagaaaaa gcttttcgac acgattatct gttccaaaaa atgtctaatg
    15361 aatgctgaat aagacagaaa atcaatgaaa ggaaaaggac tagtttatta tgaaaaataa
    15421 aaaaacgata cgtttgctag ctgctacttg gctgattgct ccgtctgttt tgtcgaccaa
    15481 taccgtattt gccgtagata atcaaatagc tgatcaaaca gtcacaacag atacgactac
    15541 atccagctca gctactagtg aaacaacgga ttcttttaca gaagacccaa ctggaacaac
    15601 taactcaaca acaggatcga ccgagccaac tgaaattgat ccggctgatc catcagaaaa
    15661 tgaaaagcca gccgagacat attcccttac aattgctcct tctttgattg gtaagatcca
    15721 actgactgct tcgacagcgg attcccctga acagcaacct ttggatatta cggaaagtgg
    15781 aacggtagaa ggtttattag cggaaacgaa ggtaacttac actattacgc cagctgaagg
    15841 tgaacaaatt gcttcagtcg ctgttaatgg tgccaaagaa gtaaattatg atgcaaagac
    15901 ctttacagta ggtacgaaca atttatctat tactgcagaa tttaaagctg gaccggatac
    15961 ttcaacagag aaaccaactg atcctagtga agaagtccct agtacaccag atccggaaac
    16021 gccaaacaaa ccagatgtgg gtaataacaa caataataat ggttcaaata attcaaacaa
    16081 taataacaat aattcgaaca gcgggaattc ttcgaatcaa acaaacccat ctggatcgaa
    16141 taacaatgga aatactcaga gagaaccaaa ccatccaaac aatggttcat cgaatggcgg
    16201 aacgaacaat gcttcatctc gtccaaatac agcaaatcaa agcggtatag agaatccatc
    16261 ttctgcttct tctgattttg ttgtaaaatc accaattgat gctgtattgc ctacaaatac
    16321 aacgaatgtc caacaagcaa tcgttcgaga agcattcaag catcttggaa acccatatgt
    16381 atggggagca aaaggtccaa atacttttga ctgttctggt ttgacttatt acgtatatat
    16441 gaaagcaacc ggtcattata tcggtggctg gacaggcgaa cagcaatatg caggtacgca
    16501 gattccagta agtcaggccc aacctggcga cctcgtcttc tggggacctt cttccggtgt
    16561 tactcaccat gtaggtatct atattgggaa cggacaattt atccatgcgc ctcaaccagg
    16621 agataaagtt cgagtaactt ctatttcaga tttcacgcct gattttgctg tccgagtaaa
    16681 cttagcaggt ttacctgctg cttcaggttc gctgacaggc ggctcatcta ttttagatgg
    16741 attagatggc tcgttccatt tcagtcaaaa ccaaacaact gatcagttct tgaaaaagat
    16801 tgcagatgat gcacaggaaa tcgggcaaaa agaaggaatc tacgcatcag tcatgatggc
    16861 tcaagctatc cttgaaagtg gatctggaaa cagcttatta tcaagcgaac caaatcataa
    16921 cctttttggt atcaaaggat catataaagg aagcagcgtg accttcaata cacttgaaca
    16981 agatagttcc ggacaaagct accaaatccg cgcacaattc cgtaaatatc cttcttacaa
    17041 ggaaagtttg gaagactatg ccgacttgat caaaaatgga ctgactggca atcctgattt
    17101 ctacaaacca acttggaagt cggaaacaaa agactacaaa gaagcaacca aatatttgga
    17161 aggacgttat gctacagatc gccaatattc acaaaaattg aacgcgatca tcgaagcgta
    17221 cgacctgaca aaatatgatg aaccgaaaaa agaagcagca acaactgaag aagcaaacgc
    17281 tgaaatcctc aatatgtcta agcgattcga tgtgccaatc aaatggacaa cagatggcgt
    17341 gccaatcaca ggattagaat tttcaacgat aaaacgcgtc aacaacgctt cttccatctt
    17401 ccgtgtcgct tcaatctggg atttgtggaa caattttacg gcaagacaga taccagaaac
    17461 aactactcgt acagttaaag ctgcaactgt tcctgtacta accttgttaa gtttagacaa
    17521 atcattctgg cttgatctgc ctttaaagaa atagaataaa ttaaaaaggg gatgtgacac
    17581 tgtcacactc cctttttaat ttattctatt tctttatatt tattttgtat tccgttacaa
    17641 actcgcgtac ttggttaggt tccagagtga catctccaaa actttcaagt cgctccgctc
    17701 ctggcagttc ttgagtctca aatgtgatac cgccatgatt tactaattta ttcccgcgca
    17761 tttccggtcc atcttctcca aaatttgctg taaaaatcac gattcctgaa gcatctgttt
    17821 ttacttggat cgatactttt tcatctgggc ttgtcaattc cgcagcattt agtaaaccag
    17881 attgtttttt caacaaaaat ggatgatcga tgccgcccac tatcattgat tggcgaaaat
    17941 cactggcaaa gacctcacct aattttttag gacgttggaa gtcaaatgct gtgccactta
    18001 ctgaaactac ttctcctgta ggcgttgtat ctttattgag cgtagcaaat aatcggctat
    18061 tcaaccaaag tatatgttcg tcgattgact gtgctggatc accggttaaa ttgaaataga
    18121 catgatttgt aggattgaat actgttttgc ggtcactgat cccttggacc gtcactcgcc
    18181 atgtatcatc gttcgtcagc gtataccgta cttctacatc taaagttcca ggaaatccgt
    18241 tttccatatc tgggctagtc tgatagaaaa tgaccgatgc ttcgttttca ccattgatga
    18301 ctgtataatg ccactgcttg gcttcaaagc taggtgcacc accatgtaaa caatgaccag
    18361 tgtccgggtc tgtttttact tcgaacgttt catcatctaa tttgaatgtg ccattcgtaa
    18421 tccgtccagc catacgtccg attgaagcac cgatataagg gtctttttct agatattctt
    18481 ttgcttcatc aaaacctaag atcaactctc gtttttcatt atcgattatt gtttgaaaat
    18541 gaacaacccg tgccccaaga tcactgacag acaaagatgt cccattttga ttcgtgatcg
    18601 taatcaagtg atagttttcg ccaaaatttt gttcattgat ttccatgttc cttgcaccct
    18661 cttttctttc atttgcactg attccagcat acatggaaac cgttttctta acagcttatt
    18721 ccgtgagtaa cgagaaaaaa tttactatat tctaataaaa gaccgtgaaa aaaaggttgt
    18781 gacaaaaata ttgtcacaac ctctttttcc gaacaaacgg tgttcaaaag cttgcatctg
    18841 cggtaataag cccaaccaaa cgaaaaatat gaggagctat ttttgcttgg ctgttcttat
    18901 tacttgatgc aggacagctt tttcacaatc tctttttcaa tacgattcca gtatataacc
    18961 atgtcgtcag tacatagtag atgaaataca atacgaagaa tatcgtaaac ggaacccaaa
    19021 tatctttgta tggattttgc atgaatagac caaacatttg taatccgaat aagacatgga
    19081 ctacacctaa gattcctggt gctaagaata acacgcctac ttctctacgg atcgattgtt
    19141 taagcaaccc gcgtcgtgtt cctatctttt caagcatttc ataacgagca atgtcactct
    19201 tgcttcctga caagatcttg aacatcaaac agctagcaag catggtcaag aaagctaaac
    19261 ctaggaagaa tcccataaat tcaaaaccag agaatagacc attatacact tcatacattt
    19321 catatttctg gttgctgtac tctacttttt gcaaagatgg attgttcatt ttattttgag
    19381 caacaagtgt ttcgattggt ttcaggtttg cttggaagtc ttttacctga actagctgta
    19441 aagtggattg tggcaagttc agttgatcaa attctgattg actgactacc ttgatttctt
    19501 tttcttgctg ttccggcaat tcatagcttc gaagttcctc ttgtacagtc agttcattca
    19561 tttttttagc aggaactttt tctttcgtga tcttcggcgg tgttccatct tcatgtttta
    19621 tcatgatcaa tggttgagcc gcaaattcac ttgcattata gtaaatcgtt cccgcatctt
    19681 ctttttgagt ataagaagca gaaagtgttg gtgaaagttc tttgattttt tgctgatctt
    19741 ctgcccgagg attattcatc actagatcat atccagaatt gttttctgtc aagtctgcaa
    19801 tttcattttt aaaacctaat cctaccgtca aagcgcccag tgccaaggca aacagcatag
    19861 cgaccattga gagcatttgt gtatattcac gaatacgaaa gtttaactgt gacaatgtga
    19921 aattgttcaa ttttttcaat gaaatctgtt ctgatttctt tagtaatgat aaaaagaaaa
    19981 tgatcacaga atggaaaatg aaataagttc ctaatacgat cgtaactaag gctaccccta
    20041 taccaaagac ttgataggtc cccacgtgtt tcatcatata gtaaccgcag cctagaaaag
    20101 caattcccag tactacttca aggaaaaaca agaatttatg ctgcttgatt cttgttggtg
    20161 tctgatccgc acgaatcaat gctaaaatcg gttttttgac aatcgaaaaa gcattgacaa
    20221 cagcagctaa taaaaacaaa acagcaaaaa acaaaacagt gatcaacaac ccgtgaatac
    20281 taaaaggtgt aaaatgcgaa atattgagat ctaactgatc cactaataat cggtggacga
    20341 tacttgtcaa tcctacaccg acagctgaac ccactaaagt agctgcgatc cctactaaga
    20401 atgtttcaga aaaaatcatc tgtgcgattt ttctgccttt tgctcccagc atcataaaca
    20461 ttgcatagtc tttttgccgc atcgtcatca aaaaggaatt tgcatataaa atataaacga
    20521 atgtgatgat ccctagtaaa acagaaccga atcggaaaat gataactgta gccgcaacga
    20581 tcgtattact tttcaaaaaa gcttcgttgc ttgccattga ttcaaacatg taaaaaatag
    20641 cagatgccat aactaatcct gagaataaca ccaagtagtc tcttaaacgg ctcttgatcc
    20701 cagtaagtga taatttccaa agcatagtca atctccccct actgttccaa gtttccaaga
    20761 atagatagta tttctcgata gaaggaatca cggctttgat ctccacgttt cacttcttga
    20821 tggataccgc catccttgat gaataaaata cgttggcaat aacttgcaga aaacggatca
    20881 tgcgtcacaa gaagaatcga tacatgatct tttgtattca attcgtccat cgtatctaaa
    20941 agatcccggg cacttttgga atccagcgca cctgtaggct catcccctaa tagaatcgtt
    21001 ggttgtgtga tcaatgcacg tgcggcggcg acccgttgtt tttgtccacc ggaaatctca
    21061 gaaggatagc tttccaaaat atgagagatg gataaacgtt ctgcaatttt ttggacacgt
    21121 tgcttgattt cttttggtct gacgccttgt aaagaaagag gaacagcgat attttcttgt
    21181 gctgtcaagc tttctaataa attgaaatct tggaaaataa agccaatctc attcgcacga
    21241 aaatctgcta gttgatttcc ttttaatgtg gtcacatctt tttgattgat tcggatatgc
    21301 ccatctgttg gtttatctaa tgtggataaa atattcaaaa gtgtcgactt accagaacca
    21361 gaagcgccca tgatcccaat aaattcgcct ttttctacat cgaagctgat gccgcttaat
    21421 gcttgcgtct ttttctcatt gttttttccg tatacttttg ttacattctg tacttcgact
    21481 attttgttca tttctttccc taccctttaa ttagtttttc cagtgctgtt ttcggcacgt
    21541 cttttttttc gacttcttca tacgaagtga cacctttatt tttgttccag gtgattttta
    21601 aatatgctcc tttacgaagc ggtcgagtca attgtccctt gaagtccagt tctttttcct
    21661 taccctcttt cgtatagcca ggcaggatat atctatagtt gactgtttct tgtccagagt
    21721 ctgattttga aactgttttt gtaccatctg tcgtaatttg tacatagtat tcttctccac
    21781 ccataactac gtaatctgcg attttcaccc ctgcaagtac cacaacgatg aaaacaagta
    21841 gaccgatcag aagttttttc actttttccc cttctctctt actttatata ctgattatag
    21901 aaaaaagcga acaattctgc cttcgaatca actatcattc ttctttcaat tttgtcattt
    21961 tctttaaaac aactaaaaaa agagttcaag acaataatcc gtcctaaact ccttcttcct
    22021 atcttacaaa aagtatgtca cttacggtct ttcatgtcgc ctgtattgat aacaggttgc
    22081 gttcctttgt ctgtaatgat tgagacaagt aaattattga tcaattggac tttccgatca
    22141 tcggtgaaat tgatttcttg tccatcttcg atttgctcta aggccatttg agtgattgtg
    22201 acagctcctt caacaatggt ttgtcgagca gacaaaatgg cttttgcttg ttgacgttgg
    22261 agcatggcac tagcgatttc tgttgcatag gctaaatggt tcaagcgtgt ttcgattact
    22321 tcgacacctg ctactgctaa acgctcttgc agctcttttt gcaactcgtc agacactgcc
    22381 gtcgtgtttc ctcgaagcgt caaatcattc tcattgaatg tatcatacgg gtattggcta
    22441 gcgatatgcc gaatggctgt ctcactttgg atttctacaa aatcttgata atacgctaca
    22501 tcgaataatg cctttgctgt atcaacgact ttaaagacta ctactgctga gatttcgatg
    22561 gggtttccat ccaaatcatt gacttttaaa acagaactat tgaaattccg aactttcaac
    22621 gaaacagtca ttttttgtgc caaaggtatc gtaaggaaaa agccattttc tcgaatcgta
    22681 cctaaatatt gtccaaaaaa caaaattact tttgcttgat taggactgac tacagttgcc
    22741 gaacttaaca acaataacgt gatcaaccaa agaaaaatac ttaaaaaaag tgcccaaact
    22801 ttcgcttgcc acatacctaa atagaagaaa aacaatccaa ctaaagctaa tacgaccaat
    22861 ccgacaatcc ctgcataacc attgatataa aatgctcttt tttctctcat gctacttcct
    22921 gcgctccttt atccttcaat aaatatctaa tgttactttt ttaatttttt tttcgaatac
    22981 gcgccattac accttctagt tctaactctt tgtcttcagt gatgtaccaa ccgcttgcct
    23041 gtgtccctcc ggctgcctgc ggtaaataat ccaattttgt tcgaacccag tgcttttctt
    23101 ctgtaaaata gtcttctacc aagacctcta gatccatccc tgctgaaaga ggcaaatctt
    23161 cccgaacaga tttaaagata aatcgttcct tcttcccatc atacttcaat attcctgtat
    23221 cgataatcgg taaagaataa taatccagct ctttcttcac cgtcaataaa tgtttgaaaa
    23281 cttcatggta cacttgctct aattggcgac cttctgaatt gttcgggtaa ttaaccatca
    23341 ctttttcttt gtctggatca tagaaggttt ggcttagttg agtgatataa gtatgtaatc
    23401 tttccatttc tgtttttaac ttttctcttt gaccttcttc taccttcata tactctttca
    23461 ccctgttttc tttttagata ttttctttct tattttacta tagttatgtg cgttaagcga
    23521 tgaaaaacag caaagattgg aaggatcttt gctgtttttt cttaaaatat ccagttgagt
    23581 acaggatcaa tctgtttcat tgtcctcttg atccgtcgta atattgatcg atgtgtcttt
    23641 agcagtgatc tttagctttt tctgtccttg ttctaacaaa gtatttgtta ttggctggta
    23701 gtctttttca atataagatt caccagacgt ttggatagta tagccactgt tttccatctc
    23761 atgcaaagat aaattgccac cgtttaggat gacttttcct tctttgctga tcaaactgtc
    23821 ttctagatgg aagttgctgt tttcacctgt gacgatcaag ttatccacat tggcctcatc
    23881 taatcgaagt gagcctaatt catctttcgc agttattgtt cctcctgtaa attcattgaa
    23941 cgatacccag ccatcatgtg tcttagtatt cacttttttg attgatactt cgttgatatc
    24001 aattgcacta ttattccctt cgattgtcaa ttcagataat tcatgatctg cagggacaac
    24061 aatagtgatt tgtgcacttg tgtcttctaa actaatgatt gcacctgttg attttttgac
    24121 tgcattcaca gtcaacacgc catttttctc ctttatttct ggcttttctg tcttatcata
    24181 agtcgttttt atctcaaaat ccaatccgcg tatgaccgaa actgtttgat tttctgcatg
    24241 gatgacgatt ttgtttgcat ccttgatttt ttttgtctca gtaaccatat cgatcaaccg
    24301 aggaccatta tcccaagtca gactcttttg agcgcctaat gtataagttg cacctgctag
    24361 gaggccacca gtaagcatca gaccaatacc ggtaatcaaa aaatgtttca atctcaattt
    24421 atgtgtcctc cttcaaacgc ccgctgaatg cagatctctt tttgtaataa aacgtcgtcc
    24481 catccattta acaaagttca tcatgacata aaaaagccct tttgttcctt tcagcaacag
    24541 tggcgaaata aatactgcgc ttcctgtggc gaacagaccg acaccgataa agaataatcc
    24601 actcagaatc gattgtccaa acacactgat tcctgagatg atcatgaata ggccaacacc
    24661 taagatactg atacataaaa catagatact aaaaatgagc gctagtaaag caaccaatag
    24721 tcccccaata accaatataa accccagtgc tagtggaatg aggacaggag aagcaaataa
    24781 accgataatg attaaccaaa tcaggcgaat ttgtcttttt ggatttttca ctaactcgtt
    24841 ttctgcttgt tcttcttctg aaaagtaact gatttttaat tttaaggcaa actgctttgg
    24901 tgtaccatat tcatcaatga tctgatctaa tgtttttcca gaatctaaaa aatattcctc
    24961 ataatactgg atcaaatcaa aacgatcgtc ttcaggaatt tcttgaagat gatccacaac
    25021 ttcattcaaa tattcattca tttgtgctct cctccaataa ccggtctaaa gaatctcgaa
    25081 aaacaatcca ttcatcaata atttcttcta aatgacgact tcccattggt gtcaacgagt
    25141 aatatcgacg gtttctgcct tcaaaaggtt catcataggt ctcaagatat ccttcttttt
    25201 tcaatcttct caaaaccgga tatagtgtag attcactgac agagagactt tcttgtacga
    25261 tttttgttaa ggcataccca tacatatctt ccttgcttaa tgctgctagc acagtaccat
    25321 caagaagaac agttggaatc tggatgtgca ttacgtcacc ttctattctc aagtgtaata
    25381 ttttatttca atctatatta tacaacatat aatatagttt caaaatcagt ttgtcaaata
    25441 caaaaaaact gactaaaaga tttgatcctt tttagtcagc aaaaaatggc ttctctttga
    25501 tttttattat aacagtggca taatccaatt tgtccacatc cagctcagtg gcatgataac
    25561 gatcattgca ggtatcatgt cagctactgg gaacattttc acgttgatca ttctaaaacc
    25621 ggtagcgacc atcaagaatc cgccgcaagc tttaaaatct aagatcatgt ctggtgtagt
    25681 caatggataa ataaattttg ccaataagaa caaaatataa aaaataatga attggggaat
    25741 tgctatcaca gaaacgacat accctaaatt acacgcaaaa atagctgctg tgaaaaaatc
    25801 aaggacagat ttagaaatca aaatcgtact gtctcctgtc atcccagaat ctaaacttcc
    25861 atagatcccc gtaccacttg cacaaaatag aacaatgacc gtcactaatg tgctgataaa
    25921 ttcatcgtgg gtcataccaa gcttttcact agggaagatt ttagaaatag gtacttgcat
    25981 cagcgcagcc cccttattta tcccattacc taaatgaaca atcaaaccaa tcgctgttcc
    26041 tatcactaaa gcaaaaataa cagccggcat atttttcatc ggcgcaatgg aatagattcc
    26101 catccccatc gaacacacac caaaaaccat cgtcagctgt gctttaaaat gttcacttaa
    26161 tttatttcct actaatccgc caattagtcc gccaaacaaa acggaaccta cattgatcaa
    26221 aactccagtt ggcatcaaaa aactcctctc tttttctacg aagcgaaaaa aatcagaaaa
    26281 atttctgtaa ttattgcttt cgctgcaaga aagattgtaa cagacagaga agattacacc
    26341 aattcatttt ttgtgttaaa gtattcttta ttggaataag taggaggaat atgatggata
    26401 tccataaatt cgaagttttt cttgatttag ccgaaacaat gaatttcacc aaaactgctg
    26461 atcgacaatt caccacccaa ggaaatattt ctaaacagat tctttcgtta gaaacagaat
    26521 tagacgtcaa attattcgaa cgcgcccata gaaaaatcga attgaccgaa gctggaagct
    26581 tattgctccc atatgtaaaa aatgtcgtag aacaatacca tgctatgcaa gaagtgcttg
    26641 aaacctatac gaaagacaac aatctttcat taaatatctt aacgataccg acaatgatca
    26701 attataaagg tttttccaaa atcaccgagt ttttgaaacg ccatcctgaa tttacagtcc
    26761 agttgaaaga agtggaaagt gtcgaattaa cttacaatga cgccaatgaa catagtgaca
    26821 cgatccactt tgctagaagt ttccaggccc caccaagttc gattgagtgg ttgcctactg
    26881 aaacagacga ttttgtagca gttctaccta aaaatcatcc tttagctagt aaaaagaaat
    26941 tggatctaag cgaactgaaa aaggagaact ttttgctcct tggccccact acgaacttgt
    27001 atcaacctgt actagatctg tgtcgcgaag ctggatttga accaaaaatc tcttataaag
    27061 gagcaagaat cgacttgatc atcaatatga ttgcaaatga catggggatt gctattgtta
    27121 tggaaaaaac agtcaagaat ctattaaaag aaaatactgt gattctccct atttctccaa
    27181 caacagagag ttatcttgca tttactcgta aagtcggtga gcattctttg gcttcagata
    27241 cattttggga gtatttaaat caagaagaaa actaagccct acattttctg tccatctatc
    27301 cacccaaaaa aagagagcga cgcatgactt tcgctctctt tcttgcatca tgtccttgtt
    27361 tcaatgacac ctctaacatt tctcgtagtt caataagaaa gcagcgattt ttatgccaaa
    27421 agaaccgtaa gaacatttcc ctaatcacaa aaaaacgact aaaaacccct aaaaaccttc
    27481 aatagaattt ttaggggttt tattcttttt cctattgttt acttaaccgt atcattgctg
    27541 tcgttcctac tccttcgata ctgctataat caatacgtcc atcatgcagt tctagaattt
    27601 ttttaaccag tgaaagtcct agcccattcc cagaaatttg acgacttgta tctccttgat
    27661 aaaatttttc aaacattcga tctctggtct cttcattcat tcctacacct gaatcggtca
    27721 cttccagtac aatctctgtg gctgtctcaa atagtttgat atgaatctgt ccattgattt
    27781 gattgtactt tatggcgtta tccatgatat tcagccacac ttggtacaaa aactcttcat
    27841 tgcctgtgta atttactgca gcaagttcga tatccaattc tagctgttct ttctcccatt
    27901 taggctgaaa aaaaacgatt acttctctga tttgttcatc taatcgatac tctttctttt
    27961 ccagttggat acgctggttt tccagtttgt tcaattttaa gatgttctct gttaattgtg
    28021 aaagctgcgt gatactttcg ataatccgat ataaaaatat ttggcgttct tcgtcagaga
    28081 gatttggggc ttgtaacaat tgaacatatc cttggattgt tgctagtggt gttttgaatt
    28141 catgggatac actggacaca aagtcattcc tcagtgtctc gatactattg agttcttgga
    28201 ccataacgtt gaaatcttta tacagttgct gtacttctgc tactttctgc tgttcatcca
    28261 ttcgaatcga aaaatctccc gttgctacca gactcatatt tgtccctagc gcactgattg
    28321 gatgcaggat acgccgccca acaaaaatcg atattcctgt acccacgacc atactgaacc
    28381 ccgcaaagac gaataacggg tataacgttg gttttccagc cgattcagca ggcgagatag
    28441 aatgtcgaga caataagaaa ataaaaccga gaaaacacag cagtgtcact aagatcgaaa
    28501 tgaagacaat actagcaaaa tagatccata attgtgaaaa tagctttttt ttcatcttta
    28561 aataaccgcc ttatagccta gtccccgtac tgtgatgatt tgaaaatctt ccgtcttttc
    28621 aaaacgggtc cttaaacgtt tgatatgtac atcgatcgtt cgttcttctg tattcgtatc
    28681 taatccccaa atatcatcca tcaactgttg gcgagtgaag atcttgttgg gataagacag
    28741 taatttatac agaagcagaa attctttttg aggcagaatc aacgaatgct gatcttgtaa
    28801 cactgtataa gcatctagct ctaattttgt ttgtccgatt atcaagctac tgctgtaatt
    28861 gatctttgaa cgtcgaagca atgcttccac acgtaggatc atctcattga catcgatggg
    28921 ttttgtcata taatcatctg ctcctaattt gaacccgcgt ttcttatcct caaaatcggc
    28981 ctttgccgta atcattagaa caggtgtgtc aaatctggtg ctacgtaatg agtctagcaa
    29041 gtcatagcca tccattttcg gcatcatgat atctgtaatg attaattcta tacgtgtcgc
    29101 ttctagtttt ttcagcgcat cctctccatt caatgcggtg atgactgaaa agcccgctcg
    29161 ttccaacaca gaacggtaaa gcaactgtaa ttgcggatca tcttctacta ctaaaatacg
    29221 attcattgat ctccctctgt tttttgaaaa atttctagat tttttcttgt agttcatctt
    29281 cagttaacat ttatttcgta tactacgatc attgttctga tatacttatt agtataccga
    29341 atatttcaat acgggagaag ggtgattatg cttcaattaa aagatataaa aaaatattat
    29401 aaagtcggcg aaaccaccac caaagcattg gatggtgtgt ctgtcgcttt cagacaaaaa
    29461 gaattcgtag cgattttagg tcctagtggt tctggtaaaa cgactatgct gaatgtgatt
    29521 ggtggactag ataattatga ttccggtgat atggtaatca acggaaagtc tactaaagac
    29581 tttaaggaca gtgactggga tgcctaccga aacaactcta tcggtttcat ttttcaaagt
    29641 tacaacttga ttggtcatct aggcattatt gaaaacgttg aacttgggat gacattaagt
    29701 ggtgtttcta aagacgaaaa acgaaaacgg gcagaagatg cattgcaccg agtcggcttg
    29761 acaaatcata tgcataaaaa gccaaatcag ttatctggtg gacaaatgca gcgagtggcc
    29821 atcgctcgag cactagccaa tgacccagat atccttttgt gcgatgaacc aaccggagcg
    29881 ttagatacag aaacaagtat ccagatcatg gaattgatcc aagagttatc gaaagaaaaa
    29941 ttagtgatca tggtcacaca taatcctgaa ctggcccatc aatacgcaga tcgaatcatt
    30001 gaattttctg atggtaagat cttaacagat tcccatcctc acatcgaacg accaaaagat
    30061 gatcaattta acttacgcag aacaaagatg agtttttgga cagcgttaaa gctttccttc
    30121 aacaatatcc gaacaaaaaa aggacgtact ttcttgacct cctttgcttc cagtattggg
    30181 atcatcggaa tcgcgatcgt tctgtccctt tcttctggat tccaaaaaca aatcgataac
    30241 acgcaagctg aaacgatggc gaaatttccg attactattt ctaaggtaac gactaaccag
    30301 actcgtgacg atgccggatt gggtgcaagc aaagcggatt atccagacag caaaacgatt
    30361 actgcaaaag tcagcgaaga agataaagca cagcacacga ataagattga tcaaacatac
    30421 gtcgattacg taacagacat cgatcctaac ttaagtaata atatcggatt tacccgtact
    30481 acaggaatca atcttttacg tgatgtaaat ggcaaagtac agcctgtcag cttctcgaat
    30541 caaaatcctg atacggaatc tctttcttta tctagtacga tgtcagcaat gacaggtgtg
    30601 ggagtgtcaa gtttccctac acagcttgac actagcaaag aaaacttttt aaaagacaat
    30661 tattctttac ttgccggaag ttatccagcc tcagcaacag atgtcgtgct gatcgtagac
    30721 ggcaataata atactaatat caacgcattg aagaatcttg gatttgatgt gaaagaagac
    30781 gaaaaacttg attttgatga tatcgttggt acaacattca aattagtaaa caacaatact
    30841 tactatacaa aacttccaac tgggaatttt atccctaata cagattatga tgcgatgtat
    30901 cagaatgctt ctgatgaatt gaagatctcg gggatcctac gcgtgaaatc ttcttctaca
    30961 atgaatctct tatctccagg aatcgcttat agcgatcaat taacgactca aatcgtcaac
    31021 gaaaacaaag aatcagaaat cgtcaaagct caaagagaca gtggtgtaaa tgtcttaact
    31081 actgaaaaag ttgacgaaaa tgccaaacaa acattgctta gttatttagg tggagacagc
    31141 ttgccttcaa gtatcatgat ttatccgaac aattttgaag acaaagaaaa gatcttggat
    31201 tatctagatg attacaataa aggcaaatca gatgaagata aaatcattta tactgattta
    31261 gctggaacga tgactgaact gactggcgga ttgatggatg ctatcactta tgtccttatt
    31321 gcttttgcag ggatctcttt agtaacaagt atgatcatga tcagtatcat tacttataca
    31381 tctgtgattg aacggacaaa agaaatcggt gtactaaaag ctttaggtgc acgtaaaaaa
    31441 gacatcactc gcgtatttga cgcagaaacg tgtattttag gtatttcttc tgggatatta
    31501 ggtgtgttta ttgcatggct agctacgttc cctatcaata gcatcttata caacatgacg
    31561 gatctaaaaa atgttgctca actaaatcct gtccatgcga ttattctagt gatcgtctct
    31621 actatattga cgatgcttgg cggtcatctt cctgcacgaa tggcagcgaa aaaagacgcc
    31681 gctatcgctt tgcgtgcaga ataaggttgt gacaaccaca tttagatttg agtcataaga
    31741 gcgctgaaat agtagaaaac agcaagaaac gaggaagtaa tatgttcctt gtttcgcgct
    31801 gtttttttta attatttttt ataattctgg tatacttttc tttcctttgt accacaactc
    31861 ttctggcgta tacctgtctt ttccatttat gaatactgtt ccatctgctt caatcgttac
    31921 ttcagtccca atccacatat tacgagaaag tgcgagaaga ttttttcttt ttcctcttcc
    31981 tttttttcca aattgcaagg catcggttat ctcttctgtc caatgagatt tcacgtattt
    32041 tacttctccg ttttcaaatg taactttcaa aatattcgct atattttcca cttttactgc
    32101 tttaggaaat gactcctcca tttttttcac tttccttcct ccaacactaa gtatgcgctt
    32161 tcttttagtg ataagtatat catagtttat tctattatca aaaaaacgca tccattccat
    32221 tgtagaatac tttaccgaac aagttgaatt gttttcctct ttttcacctt ttactataga
    32281 taggtaaaga caactaagga ggaatctctc atgacagatc aaccgtatga tttaagaaaa
    32341 gtcatcgaag aactaaaaac acttccaggt caatatcacg aaacagatat tgaaatcgat
    32401 cctgaagcag aattatctgg tgtttaccgt tatatcggtg ctggcggtac agtaaaacgt
    32461 ccgactcaag aaggaccaac tatgactttc aacaatatca aaggtttccc cggcacacgt
    32521 gtgaacatcg gaacaatggc tagtcgtaaa cgtgtaggtg aaattttgca ccatgattat
    32581 cgaacacttg gtcgtttctt gaaagacgct gtagaaaatc caatcaaacc tgtgaaagta
    32641 gacaaagcag acgctccagc acaagaagtc gttcatctag ctaccgatgc agacttcgat
    32701 attcgcaaca ttttgcctgc acctacaaat actcctgaag atgctggtcc atacatcaca
    32761 atgggtgtag tcttaggatc agatccagaa aaaacaatga gcgatgtaac gatccatcgt
    32821 atggttctag aagataaaga tacgatcggt atgtacatca tgcctggcgg acgccacatt
    32881 ggtcacttcc aaaaacaatt cgaagccttg aataaaccaa tgcctatcac tatcaatatt
    32941 ggtttagatc cagcaattgc cattggcacg actttcgaac cacctaccac tccacttggc
    33001 tacaatgaac tatggtgtgc aggagctttg cgtaacgaac ctattcaaat cgttgatggt
    33061 atagcagtcg atgaagtggg tattgctcgt gctgaattca tcattgaagc agaaatcatg
    33121 ccaaatgaaa caatgcaaga agatatcaac acgaactcag gaaaagcaat gccagaattt
    33181 cctggctata ctggggatgc taatccagct gtacaagtag tgaaagtcaa agcagttact
    33241 caccgaaaag acaatccaat catgcaaaca acgattggtc ccagtgaaga acacgtatct
    33301 atggccggta ttcctacaga agcaagtatt cttgccttgg tcgataaagc tattccagga
    33361 aaagtagtaa atgtctacaa tcctcctgca ggtggtggta aattgatgac catcatgcaa
    33421 atccgtaaag aaaacgaagc ggatgaaggt attcagcgtc aagcagctat tcttgcttta
    33481 tcagccttca aagaattgaa gacagtcatt ttagtcgatg aagatgttga tattttcgac
    33541 atgaacgatg tagtttggac tttcaacact cgtttccaag gagataaaga tatcatcgtg
    33601 atcccaggaa tgaggaacca tccattggac ccatcagaat taccagaata taatcctggc
    33661 tctattcgtt tccgtgggat aagtgcaaaa actatctttg acggtacagt tcctttcgat
    33721 atgaaagaac attttgtacg tgcaaaattc caagaagtag aagattggga aaaatatttg
    33781 aaataatgaa tgatctgcaa ctgttttgac atcataaaaa ggagtgcgga aaatggaaca
    33841 attggcacaa cggaagaaaa aaatcgtgat tggtgtaagt ggcgcttcag gcaccattta
    33901 tgccatcaac ctagtgaaga aactaaaaga atatccgatg atagaaacac acggaatcat
    33961 cagtccatgg gcaaaacaga acttaaagct ggaatccgac ttatcactaa ctgaattcga
    34021 aagcctgttt gattaccatt attcaaacaa agatttaggt gctgctgtcg ccagcggatc
    34081 atttttgacc gatggaatga tcatcgtacc ggcaagtatg aaaacagtcg caggtatctc
    34141 tgttggttta ggcgacaacc tgattgcacg tgctgctgat gtaacaatga aggagcaacg
    34201 aaagttagtc attgttcctc gtgaaacgcc attaaatgcc attcacttgg aaaacttgac
    34261 taaattagca cgactaggtg tgcaaatcat accaccaatt cctgcttttt ataatcagcc
    34321 tcaaacgatc caagatcttg ttaatcacca tacaatgaaa cttttggatc aatttgaaat
    34381 tgtaaatgag cattcaaaac gttggcaggg tgattaatat ggaaaaagaa acgatagttg
    34441 aagtaaccaa actaggtttc acgatccaag ctgctgttaa agtaatcggt aaagacttat
    34501 tgatcacttt aacaggcgga gatacaccac atatcggaac agtcactaca ttttctcgtg
    34561 atacagagat gcaaactgta cgtttcccta gccacgatgg tcggttccat aaagacgatg
    34621 tcttagccga aaaaatagct gtgatcatca aaactgccgt tccgggaagt tgtacgatca
    34681 cttccggtat ccatgttgac catatctcca atgcacaaat caacgcctct tttccgatgt
    34741 cagaagagtt aggttacaag attttagatt ggttggaaaa aatagatttc catatcaaag
    34801 aaccagttta ttataaagat ggtgaaaaac cattatgaaa gaagaggttg tgaaatcagc
    34861 gttatgacct gagtatgaga gaaaaaaatg gaaaaatagc ttctcatatt ttgccgtttt
    34921 tgagctaata ccgagggaca gctgattgaa cgccgtttat caagaggttg tgataaagcc
    34981 gcttcgtttc gagtagtaag aaaaattttg taaacatggg tttccacatt tttgcataaa
    35041 tttgacttac taccgagaaa ctggcttttg aacaccgttt atcaagaggt tgtgacacgt
    35101 cttttgtcac aacctctttt atttttgctt cactgtaact gctacaatat caccgatttc
    35161 ttttccgatg tttttacgaa tatcttttcg tagtccaagg atgtagcaga ctgaaccatc
    35221 tggatttttc acgcccatat tcacgatact gccttcatat ggcacattgt caaaagttgc
    35281 ttggacttta actcttccag caccgaattc ttttcttata tcatacggaa aaataatata
    35341 tgccccgccc ttgccgactt cggaagcata gatttttgat tcaaaagaat aaatttccct
    35401 cattttattt ctcctatttt ttacatctag tatagcatag caaaaaaaag aaggaactgt
    35461 gacaaaagtc ttgtcgcagt tccttttttc gaataaacgg tgttctcctg ccaactcctc
    35521 ggtcacaagt cacaatagtc aacaaacatg agaagctgtt ttttgaaatt gctccttgtg
    35581 actcagagtt aaacggcagg ctcacaacct cttccttaaa cggtgttcaa aagcttgcat
    35641 ctacggcact aagcccaatc agatgaaaaa tatggagtgc tatttttatc tgattgttct
    35701 tactgcttga tgcagagcag ctttttcaca acctcctaca tggtgttcaa aatattattc
    35761 gccttttgct tctttcacta attttgcgaa tgcttgttgt tggaattcag cagctaaggc
    35821 tttcgtacgt tgttcttgct ctgctttgct catttttgga ttttcagtca ttgcttttac
    35881 cataccgtct tgaacataag cttttgcttc actttccatc gtactttgct gtttcttcac
    35941 taattcagct aacttggctg cttggtccgt acttaattct acccatgtct ctgggatttt
    36001 gaatgtattc actcggctgt catattcgtg attgttatca gcaatattga accagaattt
    36061 atacatgtcg tttttgtatt cccaataagt agttttcgtc actttttcag catcgccgtc
    36121 tgtcttttct actttatttg tttcgttatc cgtaccagtt gcttttggtt tttcttgttt
    36181 ttcatttgtt cggtaaagat acactttttc ttttccattc cctaaagctt tatacaaaag
    36241 catatctgct ccttgggaat cagcactgga aacaattttt tctgtttttg tttccgttac
    36301 tttttccatt ccaaagtgat tggacaaatt agcgacgatc aaacttagtg aagcgacgaa
    36361 gattagtccg aaaatcaaag ataaaaaggt ttgccatgtt tttttagcga acacgttcgt
    36421 taaagcaaaa gcaagcaagc tgacaatcaa aactccaata atcatgcatt atcccccgct
    36481 ttcattagaa caggttcttt tttcttcaat aaaaaggcaa taataaagcc aagaatccca
    36541 aagatgatcg ctacgataaa cgcggcatgg taacctgaca gtgtggcatt gattgcctga
    36601 tctttataag ctaatggcgt attatccaat acagatttgc ttgggatatt tgcttttgtc
    36661 acattcgtca acacactgat caaaatagct gtaccaatcg agcttgcgat ttgacggaat
    36721 gtattattta ccgctgtccc atgactgatc aagttatttg gcaatgcatt cataccagaa
    36781 gtcgttaccg gcatcatgac catggagatc ccaaacatac ggatcgcata aaggatcacg
    36841 atatataaaa ctggagtcgt ttttgtcaag aaagcaaatg gtgcagttgc cgctgtcaaa
    36901 atgaacatcc ctgtgatggc taaacgtttc gcgccgtatt tatcgaaaat cgcacccgtc
    36961 acaggcatca tagcccccat gatcaatgca cccggcaaca acatcaagcc agaatggaat
    37021 gctgattctc cacgaatatt ttgaatatac aatggtacga ccatttctgc ccctaccatc
    37081 gccatatttg ttacaccagc taaaacagct gcaatcgtga atgtagatga tttgaataca
    37141 cgaagttcca agaatggatg ttccaaatgt aattgacgcc atgagaacaa ggcgatgaaa
    37201 atcacaccaa caactaagaa accaattact ttcaagcttc cccagccgtc atctcctaca
    37261 ctagaaaatc cgtaaagtaa actaccgaat ccaatcgttg ataaaatagc ggataaaata
    37321 tcaatgctag gatttgacaa tggaataaca cttttcatta ggaagaatgc taaaaccaag
    37381 acaagaacaa cgattggcag taccatacca aacaagtcac gccaatggta gttatcaatg
    37441 atataaccag ataatgtagg gcctaaagca ggagcaagtc caataacgat ccctgacatc
    37501 cccattgctg atcctctttt ttctggcggg aaaatagata gcatgatatt ttgcaataat
    37561 ggcatcgata ctcccacacc gcaagcttgg atcaaacgac cgattagtaa tgtactaaaa
    37621 ttaggtgctg taaagcaagt aatcgttccg atcaaaaaga tcgtcatggc agaaagatat
    37681 aaatttcttg aactgaattt attgatcagc catgctgtta ttgggatcat gatcccgttg
    37741 actagcaaga aaccagtcgt taaccattgc acatcagacg ctgaaatatc gaaagctttc
    37801 atcaatgtag gaaaagctgt cgtcaataag gtctgattca aaacggtaca aaatgtacca
    37861 attaataaga caacaactaa aagtgtccga ttatatggtt ttccgtaaat atctacgggt
    37921 tggttgttac tcatatttct ccttctttct gaattttttc tgaaactact agaatacttt
    37981 agtcctaaat tcgctatttg tcaacctact tgacacataa gttaatacgg atataattaa
    38041 gggaagcaag aggacaaaag gagtgacaaa aatggttggg gcttccctta aacgtttgtt
    38101 ggaaaatgat caattggttg ttggcatcag tgaattaagt gatatcgtag gtgtgtctcc
    38161 acgtcaatta aggtattggg aacaaaaggg attcattcgt tcgatcgaag ctgatgcgaa
    38221 ttgtcctaga aaatatcgtt tacctacagt cataaaagta gaactgatca aaaaatattt
    38281 agatgacgga tttactttgt caaaagctgc agaaaaggca gaaatccgcc aaaagaaaat
    38341 gcaccatgtc cgtaaagttt tttcaaaagc aatcaaagat atcgaattga tcgacgaacg
    38401 ctatacgatt ttacaaatcg gtaacttcaa agaagagaat gctaagatgt acatcattca
    38461 tgacaacgag acagacgaat taacttacaa gctccttcct gtcgatgaaa aagctgacta
    38521 cgaacagttg ttaaaagaac tctaaacgcc actaatctcc taagtaccaa gctttttaat
    38581 aagtagatgc tacataaata cttgaaaaaa ggcctgtttg aacataagaa tatccgaacg
    38641 gtgatctgcg tttctttcat atagaaagga attctccatc actgttcgga tattttttta
    38701 tttttttcgg ttactttctt ctaatacatc tgttagccaa tttgcatatt ctgtttcccg
    38761 attgatcgca tgcgctaaaa tcaggtagtg tccgtaattt tcatgttttt ctttttcagt
    38821 aggaaagatc gttttttttc gctctaatag atgaagtcgt ttttcttcat gaaggtcacg
    38881 ctgctgctga ataatttcta ctaatgccgg atcattcacg tctttgacga aatacaattt
    38941 cagaacaaat tcgtctcgat taaccggtag ttcattggtg ggtgtatgaa tccactctgc
    39001 caattgttcc ttcccttctt ctgttaattc ataaactttc ttttccaatt tttcacctgt
    39061 gatctctact tgatgctgaa tgattccttg ctcttccatt tttgcaagtt caggataaat
    39121 ctgactatgt tttgcttgcc aaaattctcc tatttcattt ttgaacgatt gattcaattc
    39181 atatcctgtc atcttcttct tacttaataa ccctaacaaa atatatcgaa gtgtattttt
    39241 tcttgccatc agttttccct ccttgacaaa tctttaaatc cattctatca taaatgagta
    39301 atcgaaaaca tgtagtttat tacatgtttt aatttataag gagatgctta aacatgaaag
    39361 aatttaaaga attggaagat tttattggaa ctcactttat ctatacgtat gacaatggtt
    39421 gggaatatga atggtatgcc aaaaatgacc atactgttga ttaccgtatc catggcggaa
    39481 tggtcaaagg ccgttgggta aaagaccaag aagctgatat cgttaaattg acggacggcg
    39541 tattcaaaat cacttggaca gagccaacag ggacagacgt ggcattggac tttatgccaa
    39601 atgaaaagaa acttcacgga atgatctttt tccctaaatg ggttgaagaa catccagaaa
    39661 tcactgttac tttccaaaac gaacatatcg cagaaatgga agcagctcgt gaaaaatatg
    39721 aaacttatcc aaaattactt gttcctgaat ttgctacgat cacttatatg ggtgatgcag
    39781 gtatagacaa tgaagatgtt attagcgaag aaccatacga tggaatgcca gatgatatcc
    39841 gtagcggaaa atacttcgat gaaaattata aacgagtaaa ataaggttgt gaaagagagg
    39901 ttgtgaaatc agcgttctga cctgagtatt agagaaagaa acggaaaaat agttcctcat
    39961 gttttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttatcaagag
    40021 gttgtgaaat cagcgttatg ctttgagtaa taagaacgct agttagaaaa tagcttttca
    40081 tattttctag gctagtgggc ttattaccga gaagcagccg tttgaacacc gtttatcaag
    40141 aggttgtgaa atcagcgttt tgacctgagt attagagaaa caaatggaaa aatagttcct
    40201 catattttgc cgttttttga gctaataccg agggtcagct gattgaacac cgtttatcaa
    40261 agattgtaac aactgcatat tcacacagtt aaattagtga cttcaaaatg aaataatgct
    40321 cccagcaaaa aattaggctg ggagcattat ttcgttattc tgttcatcaa aaatcagcgt
    40381 tttgattcca tttcatcaca agttcctcct catttactgc ttcatctttt tgctgtgttt
    40441 gaattgtaaa accttgtgaa agatagaaat gaatagctga ttgatttttt tgatagactg
    40501 tcaaagtcag tgttttattg gtttctttta tttttttcat tagttgcgta ccgattcttt
    40561 gttgccggta ttcccttttc acaaagattc ctgcgataaa ctgatcaatc accccagcaa
    40621 aaccaataat ttcttgatct atttctgcta agtaaatcac tgcatctggt aaagctttcc
    40681 taacagattc tacttgattc tcccaataag ttgatggaat gaattcatga gaatccaagt
    40741 tagcatgcag ccaaatcgat agaacagatt ccagttcttt ggcagacaat tttgttgttg
    40801 tgcggatcat tttttctctc cttacttatt cattgatttt aaaataaaaa ccattttttt
    40861 gaagctcttt tacaagtttt cgcccctcaa aaaagtatat cttctcttgt tttacttttc
    40921 taataccaaa agaaacagca actttaggat catcagctga aacgattcga taagttccac
    40981 caggcaattg atccagcttg actttcccat gatggtcgct ttgtactgaa aacggcacac
    41041 catttctagt aagtgttttt ttcttatgtt tcaaaatgaa tgaactacca tttaccggtt
    41101 tccctgacga atcactaata ataaatgaaa aatcaagcac ttttttactt aatctatact
    41161 gatagattag tcgtccgata aacactaaca gtatgactac agctattatc gttcctgcaa
    41221 gaataaacca ttgccgcatg gttctttcct gatttccttt tccttcttct ttttttaccg
    41281 attcgttata aggaatccga tgtcctgtca ctagcaaacg atgcgtgttg atcatatacg
    41341 gcgtacatgt aagcaaggta actaagtctt ctccttctga aatcgttaga acagacgtgt
    41401 catctggcag taccacttgt atatttttaa cttgataagc caatttctcc cccaaaacag
    41461 tcaaaataaa cgtgtctccg ttttctaaac gatctaattc ggtaaataac cgtcgattag
    41521 gtaaaccgct gtgagcagag atcactgaat gcgtcgaatt tccaccaagc gggaaagagg
    41581 ttccttgcaa gacgcctgct ccattttcaa ggatcctacc attgagccga tcataaagtg
    41641 aaatatttaa ttggatctta ggtatattga tacttccgat cagccattcc tctaattggc
    41701 tggatgttgt actttcattt ctgttctttt cattgaaagg atcagtacca ggattgaatc
    41761 cttggtcagt taatcgttgg tttttctcct ccattttctt tcgctgcact tcaatttcag
    41821 ctgcggtccg tttttgtact tgttcgatac gtttttgatc aataaaacta tttaaagaat
    41881 ccacataaaa tggatataaa gcgatcagta accctacgac gatacatatt aataaaaaca
    41941 aacgaaacaa aagtcgacga gactgttttt gcttactctg cttcacatgt gtcctccctt
    42001 gcaagttttt ttgttctttc ttttattata tgggttcagg aaaaaaatag aaaatgaaag
    42061 ccgatgaggt tgtgaaaaag ctgtcctgca tcaagtaata agtacagccc aaaaaaatag
    42121 ctactcatat tttttgttgg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    42181 ttaaggaaga ggttgtgaaa actgcgttat gctttgagta ataagagcgc tagtcagaaa
    42241 atagcttctc atattttcta ggctagtgag cttattaccg agaagcagct gattgaacac
    42301 cgtttaccga gaggtcatga aaaagctact ttgtttcgag tattaagaaa aattctgttt
    42361 caaaacaagg agatgcaaca aaacttatgt cgcatcctct ctccttagaa ctttcactta
    42421 tgtttttctt gtaaaattca taaatatttt catgaatttt ctaatttctt gcctacctct
    42481 cgcataggat atatgatgaa tttatctaaa gtttgcaaag gagttcgaca attgaaaaaa
    42541 tatggcccat ttttagcaat ttttttcatt gctttggcat ttctcatgcc tcatttattc
    42601 acacataaac taattttggg tgctgatgcc ccattccatt acaatcggtt ctatgatgcc
    42661 gcgcaacaaa ttaaatatgg ggatttccat tattttcctt ccctttatgg attccagcaa
    42721 agtggcagga tcgtgaatgc cgtttacggc ccatttttcg cttatttaca aggattgatc
    42781 gtgttgcttg caggtagctg gtttcattat cagattcttt caaatttcat tctttatctt
    42841 atttcaggat tttctatgct gattttactg agatatgtca atgttcgaaa ttgggtaagt
    42901 gtaccagtag ccgttttatt catgagtacg tattctattc aatactggtc tatcaaccaa
    42961 ggattcacta gctgggggac ttgtttttat cctttatgta tgatccctat tgctgatttc
    43021 gtccttcata aaaagttccc tgttatcaaa gtcgctgtct cagtgggact tatgacacag
    43081 atccatttac tgtcttcgat catgttagct ttgatgtata tcccttttta tctttattac
    43141 ttttttaacc agactgaaaa aggaaaagca ctgcttcaac tgattaagtc ggtcttgctt
    43201 tattttgttc tgacagcaaa tatttgggca ggattagcta ttgtttacag tgggaataag
    43261 attcttcctc cctttgttaa cagagaaatg agtcaaaagg cgatagatct ggatggcagt
    43321 tattggctcc attaccctaa ggcctttcct atattgatat tatcaggttt tttgcttttt
    43381 attttctttc aacaaaagaa caccttattc caaaaaatcg tgttgtacac aagtcttgtt
    43441 ttcttttttc tttctactag cttgattcca tggacgacat tgatcgaaaa aaatgtgcct
    43501 tttattagtt tgatccagtt tccatttcga tttttcgtgc catttacgct attgcttcta
    43561 ctttaccttg ccctttcact gaaccagtgg tctgaacaaa agtggttccg tcttttaagt
    43621 atatttggca ttcttatttt ttcagcacag acgatccaga acttatacca gcatctggaa
    43681 aagtgggaaa atgaaacatt tgtcagtcga catacttatc tattcgatac gccggaagaa
    43741 gcaaggaaaa cattcttcag caaagatatg tctaccagct tgttcgtttt ccaaaaaacc
    43801 acacctgact atctgccaat ctataaggag acaaaagaca ataaatacga ccggtataac
    43861 gaagaaatac tagaaaacga aacaactttt ataaaaacac atccaggcgg tcttttgaca
    43921 atcaagtgga aaaacactgg ggacaaaact gttcatatcc ctgttatcgt ctacgatcgg
    43981 actgtgctac agcaaaatgg aaaaacattg actgattatg aagtaacaga tatcgggacc
    44041 ccaatcgtga agcagcaaaa aggaatcaac gagctcacgc ttcattatca aacgccaatc
    44101 tatttctatt tcattctttc tcttactttg attggctggt ttaccttgct atgtttattt
    44161 atttatcata gatacaagct tttacgtgct taggcaaaag ggataaatac atccgatcaa
    44221 aaaactctcc aaacggtata atcgtttgga gagttttatt ttaatcagtt tttttatgtt
    44281 atatgttata ttttttattc gttcacatct aattcgattt ccaccccgta gtgatcactg
    44341 atgattggac tatcgatccc atcaaaaatc cgttgatatt ttttaacctg cattccttca
    44401 gggacgaaaa taaaatctat acgcaatttt tctgtatttt gttcccaacc atctattttc
    44461 ttttccacag tagctgcttt tcctttttca ttcgcgacaa catacgaatc ttgaagaaga
    44521 aggcaacttt ctcttacagt ataataacct tgagagtcta ctggattatt gaaatcacct
    44581 aggaaaaaaa gtggtacgcg agtttccaaa aagtagtttt ccaagttttt ccactcaaaa
    44641 taaaagccac tttttttatc tgtccaccaa gaaaagtggc agctcgagac aaaaatgtcc
    44701 tgatcatcta tctttgtttt cccaatcaag atcttgcgtg tctgataatc ttctttcttt
    44761 tgaaattggg agacggtaag gacctgactt tccaatgttt ttttcgtaag cagtgcattt
    44821 ccttcttcat atttatcata accgatgtgg ctcatttccc aactccaaaa atactctttt
    44881 ccgtgttcct ttaaaagctg aactaaacgg taggcaaaat tatctttacg gataggtact
    44941 tgattcttta ccggacagaa cttcattagc ttcgcaggtt ctacttcttc agattccagt
    45001 aattggttga tttcttgtaa agcaatcact tcataatctt ctaacaggat cttatctgct
    45061 aattcttgca attttttctc tggttctggc tcaagccagc tatgcgtgtt gagtgtcatg
    45121 aatttcattg gttttctcct tgtcttcatt tcgaaacaaa agggttgtga caaaattttt
    45181 gtcacaacct ctccagcaaa tagtgttctc ctgctaactc ctcagtaata agctcaatgg
    45241 accaggagct attttttgtt tggctgtttt tatcacttgg tgcagggcag ctttttcacg
    45301 acctctccac taaacagtat tcaaactgct gcttctcggt aataagccca accaaacgaa
    45361 aaataggagg agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
    45421 acaacctctc cttacatttt aatggagccg atcactttac cagctgtcac tgtacctaat
    45481 tgcttgatca ctacttcttc tactttatcg tttgtaaata cgatcatgat cgttggatct
    45541 ttttcttcgt tttggatcac atcaagatcc atctcagcta atttcgttcc tgttcctact
    45601 tcttggcctt ctttgacagc aatctcaaaa gctggctgat tcatttggac tgtatcgatt
    45661 cccatatgga tcaaaacttc gataccatct ttcgtttcca gcccaattgc atgtttgcta
    45721 gggaaaatac ttgtcacttt tccagcgatc ggcgcaacaa cttctcttga tgttggaata
    45781 acagcaaagc cgtcacccat cattttttgt gagaataccg gatcttttac ttcagataaa
    45841 ggaatcactt ctccagaagc tgcggaaact agttcttgtt ctatacctaa gtattgctta
    45901 ctacctgctt gtccatttcc tgtagaaacc atttctggct ctggaatctc tgctcctgat
    45961 tgcagcagat cttcgatatc tgatttcaag acatctgctt ttggtccata aactgcttgg
    46021 actccattgt ctttgacgat caatcccatc gcccctgctc gtttccaggc ttcttcagtc
    46081 cctacttttt ctcgatcatt tacacttacg cgtaaccgag tcatacaagc atcgacatct
    46141 ttgatatttt cttttcctcc cagcaaatga atgatctgga ccacttgctg atctgctgtt
    46201 cctgttccgc tgttagatac aacggtttct tcactgtcat tgtcgtaatt tccgttacgt
    46261 cccggtgtag caaagttgaa ttttttgatc aagaaatttg ctaagaaata cgtaacaaca
    46321 ccaaaagcga tcacacacaa cacgaagttg ataagatctc cacctagacc cgctttgata
    46381 gctaacggtg tccgagtcag caattcgata ttcccaaatg aatggacccg caatggcaag
    46441 atatctgcca tcgcaaatgc agccccttgg atcactgaat aaatgacata taatggcaca
    46501 gcagcaaaca taaacatgaa ctccaatggt tctgttacac ctgttagaaa gacagccaac
    46561 gctgctgaaa aatacattga tttatatctt gctttcttat caggatcgac attacgatac
    46621 atcgcaaaag ccattcccat caagatacct gaagaaccaa tcatttgtcc tactttgaaa
    46681 cgagccggcg tccagttatg caacacaaac tcatattgcg acatatcccc tgcaccttta
    46741 agattcacta aatcagtggc ccaagccaac cataacggat cttgcccaaa tacttgtgtc
    46801 cccgcctgtg cgcctgataa aatctcatac gatccgccaa gctgtgtata gttgatagga
    46861 atcgtcagca tatgatgcaa tccaaatggc aatagtaaac gttctaatgt tccaaataaa
    46921 aacggtgcta gaatcggtgc gctttcttgt gattctgcaa tccataaacc aaaattattg
    46981 attccagcct gtacgtttgg ccacaccaca gcaagaatca atgccacgat tgttgaccat
    47041 aagataacaa caaacggaac aaagcgcttt ccattgaaaa atgacaaagc atccggcaat
    47101 tttctataat tatagtactt attataagcc attgccccga caaatccagc gatgatccct
    47161 acgaatactc ccatattcaa agctggtgct tccaatacac ttgtaaaaaa tcctttaacc
    47221 atgatcttcg taccgaacag agtgtgggta aatgcatctt cattcgcaag catgtcactt
    47281 gtgacaccaa agatagcccc agtgatccga ttgattaaaa taaatgagat cccagctgca
    47341 aatgctccgc ctgctctttc ttttgcccaa cttcctccta ttgctaaagc aaataaaata
    47401 tgaagattac caataattgc ccaaccaata ttttcaatta ctccgcccgt tgtgacaagc
    47461 ggcgtccaat caggattgat caagggaatt gatttcccta tactgatcat caacccagct
    47521 gccggcataa ctgctaccac aaccattaaa gcctttccaa atttttgcca aaactcaaaa
    47581 ctaaatatct ttttcattcc gtttagctcc tttacgcaat cggtttcgtt aaacgtatta
    47641 tagcgtgcgt tttctttttt gtaaatgctt tattttttat ttttctgtct ataccattct
    47701 ataaaaccta taatacgtgt gttcccttct ataattttct cctaaaaatt tattttttta
    47761 agcttttttc tatgaaatat tttcttttgt ctattgactt gtgcaatcgg ttgcgtttat
    47821 tatgaacgta taaaacagct cattaggagg agaaaaatga aacagatcaa acgactattt
    47881 gctattgatc catggaaaat cagcacgaca caattagata aaaaaaatct tcgactgcaa
    47941 gaatctttaa caagtattgg aaacggttat atggggatgc gcgggaactt tgaagaaact
    48001 tactcagggg accaccatca aggtacttat ttggcgggtg tctggtatcc cgataagaca
    48061 cgtgtcggct ggtggaaaaa tggttatcca gaatactttg gaaaagtgat caacgccttg
    48121 aactttatcg cgatagatat ttcgatcaac gatcagctga tcgacttggc ttctttggaa
    48181 ccagaagact tttactggga attagatatg aaaaatggtg tattatctcg tacattcaca
    48241 atcactactt ctactaataa agtacgcatt tccttcgaac gttttctgag cattgtcaaa
    48301 aaagaatcag cttatatcta tttaactata gaaatgcttg aaggtagcgg aacagtcaaa
    48361 ctcgtatcaa aattggatgg aaatgttcaa aacgaagaca gcaactatga tgaacatttt
    48421 tggaatgaga ttgatcgtgg aactatagaa gaaatcggct atttgacgac taaaactatt
    48481 ccaaacaact ttgggattga acaatttact gttactgcag caatgcgcca tttcgttaat
    48541 ggaaaatcag tcatccctga ctatcacgaa gatactttag cattacatgg ttgcttgtct
    48601 gcagaattat ctcaaggtga gacatggtca gtggaaaaag aaattatcgt tcttactagt
    48661 agagatattc cagaagcgca gcaagcagaa aaagctgtct cagaattaac gaaccagaca
    48721 aacaattata tccaagcaaa agaagagcaa acagcggctt gggcagaacg atggaagctt
    48781 gccgatgtgg tcatcgaagg agacgaagaa gcacaacaag gtattcgatt caatttgttc
    48841 caactattct ctacttacta cggcgaagat gaacgattga atatcggccc caaaggattc
    48901 acaggagaaa aatacggcgg agccacttac tgggatacag aagcgtatgc tgtccctctt
    48961 tatcttgctt tagccaaacc tgaagtaact aagaatctgc tgaaatatcg gcataatcaa
    49021 ctgccgcaag caatccacaa cgctcagcaa caaggattaa aaggcgcgct ttatcctatg
    49081 gtcaccttca ctggtgtcga atgccataac gagtgggaaa tcacattcga ggagattcac
    49141 cgaaatggcg cgattgccta tgcaatctac aattatacga attacaccgg cgatacggaa
    49201 tatttgaaaa atgaaggact ggaagtttta gcagaaatcg cacgcttctg ggcagaccgt
    49261 gtccattttt caaaacgagc aaataaatac atgatccacg gtgtgaccgg cccaaacgaa
    49321 tatgagaaca atatcaataa caattggtat acaaatacga tcgcagcttg ggtcttgcgt
    49381 tacacgcgag aaaattattt aacttatcaa aaagaagctg ctatcactat cagcgaagaa
    49441 gaactagcca aatggaaaga tatcgcagag aacatgtact tcccagttga cgaagaatta
    49501 ggaatctttg tccagcatga cacattctta gataaagatc tcatgcctgt atccgatatt
    49561 cccacaagcg atcttcctct gaatcagcat tggtcttggg acaaaatcct tagatcttgc
    49621 ttcatcaagc aggcagatgt actacagggg atttatttct tcaatgatac ttttacaaaa
    49681 gaagaaaagc aaaaaaactt tgatttctat gaaccaatga ctgtccatga gtcttccctt
    49741 tcgccaagta tccattcgat tttagcagca gaattaggaa tggaaagcaa agctgtagaa
    49801 atgtaccagc gaacagctcg cttggatttg gataattaca ataacgacac agaagacggc
    49861 ttgcacatca cttccatgac tggtagctgg ttgtcgattg tccaaggatt tgctcaaatg
    49921 aaaactgccc atgatcagtt gagattcgca ccatttctgc caaaaacttg gaaagcttat
    49981 tctttccata tcaattatcg cggacgttta ttgttcatct cagtcacaga tgaacaagtt
    50041 actttcgagt tattagaagg ttctcctcta gaaatttacg tctatgaaca gtcttattct
    50101 ttagcggatc gtctagaaat aacgctaaga aaggaggaag caaatgttta aaggggtatt
    50161 gtttgattta gacggtgtca ttaccgatac tgctgaattc cattatcgtg cttggcgaaa
    50221 gcttggtcag gaaatcggca ttacgatcga ccacacattc aacgaacagc taaaaggcgt
    50281 cagccgagaa gactcactct ctttgctttt agcttatggc ggaaaagaac actctttttc
    50341 aaaggaagaa tttgcagaat tagcgaaaag aaaaaacgat tattatttag agatgatcca
    50401 gacgatcgaa ccaaaagatg tcttccccgg ggtcgtgcct ttactggatt ctttaaaaga
    50461 agaaaaaatc aagattgctc ttgcttctgc aagtaaaaac gggccattcc tacttgaaaa
    50521 aatgggtctt acaccttact ttgatgctat tgccgacccg gcagaagcaa caaacggaaa
    50581 acctgcgcca gatatcttcc tccttgcagc aaaagctgtc ggactaaaag cagaagaatg
    50641 tatcgggatc gaagacgcgc aagccggtat ccaagccatc ctctcaagtg gcgctcaacc
    50701 aattggcgtg ggacgtgctg aagaccttgg agaggagatc ccgcttgtcc ctgatacgac
    50761 ttttcttacg atcgattact tgaaagaaaa gtggcatgac catggataat ccagagatct
    50821 ccgtcgtacc agttccagag actgaaataa agaaaatcac attgaagaac gactacttag
    50881 aagtcgttct tctcaattac ggtgcccgcc tgcaccaaat cttcgcacca gacaaagaag
    50941 gaaaatcaga aaatgtttta ttgtcttacg atcgtttcga agatgttcta acagacaaaa
    51001 gcttttttgg agcaactgtc ggacctgtag ccggaaggat ccgggatgct tcttggggtg
    51061 agcatagatt ggagaaaaac tgcggatcac accatatcca cggtggcacg aaaggctggt
    51121 ctttccaatt ttgggaagta gaagttttca aaactccatc atcaataggt gtggtctttt
    51181 acttaaaaga tgaattttct acctatccag gaccaatcac tgctacgatc acttaccgtt
    51241 taacaaaaaa cgaattagag atgacaacct ctgctagtag ccttgttgaa accatctgca
    51301 atccgaccaa ccatgcctac ttcaaccttt caggaaatgg aacaagagac atctatgaac
    51361 atcagttaac ggtcttttta gatggtgtgc ttgaattaga tcaagaaaaa ctgcctactg
    51421 gaaattggac aaaaaaagaa aaactgccaa tcgactttag aaaatcccca acactgcaag
    51481 agatccttgc atgttatcca gatggactag atgatgtatt tcttttgcat catcctaggc
    51541 tttccaaaac ttccttacaa ttattcgaga agcattctgg tcgccaaatg actatagcaa
    51601 cttctaataa aagtatggta ttattttcta cgactggatt cgaagctgat ttctctgtca
    51661 atggaaaaca gatgcattca aactacggat tagcaatcga accgcaagaa atccctgata
    51721 tcgttcattt ccccaaattt ggatcgatca acctccatcc tggtcaagaa cggatcagtc
    51781 agacgattta tcggttctct gcacaataac aagagaaagg tgtttttatc atggctatta
    51841 ccgtaaaaga tgtggcgaaa aaagccggtg ttgccacctc gaccgtctct cgtgtcatca
    51901 acgatcatcc tagtatttcc gagacaacaa agaagaaagt ccgaaaaatt atggatgaac
    51961 taggttatgt tcctaacatg actgcccgca atctaggaaa acgcatttcc agtgccatcg
    52021 gcgtaatctt accccctctt gattcaaaag agcggatcgg taacccattt tatcttgaga
    52081 tcatggaagc aatcaacgaa gaagcgcgga attatgatat gactacagca atcgctactg
    52141 ctaaaaattt tgaattgctg ttagagaacg tccagcgtat gcatttacaa aaacaagtag
    52201 atggttttat tctagtgtat tcagatagtc aagatactgt cattgattat ttataccaaa
    52261 accacatccc tttcactttg atcggacagc cttatcatca tgagaacgaa atcgtttatg
    52321 tagataatga taaccagctt ctaggtaaac aagcaacaga atttttgatc cagaatggac
    52381 ataagaatat cctgtttgtg acaaacacga ctcatgaaag tttgtatttc gaacgatatt
    52441 tcggttatca aaaagcaatg atgatggctg gtcttcctgc gttatccagt gtagtactag
    52501 agcatccgga ggattatctg tcctttgaga aaacgttgaa agaaaacgaa gccacagcat
    52561 tggtggtcat tgacgatatc ttcgcccttc gcacgatgca gttggcgcaa atgtatggct
    52621 atgtcattcc agagacactc tccgtcatta gcttcaacaa ctcgatcttc tcaacattaa
    52681 cacaccctta ccttacaagt atcgatatcg atatctccga attaggacgt gtcgctatgc
    52741 aaaagctgcg cgaattgatc catgaagaat tgagcagtgg cgtacgtttg gtcatccctc
    52801 atcgcttgat caaaagagag acagtcgttc ctttagaaaa cccatactaa atagctgact
    52861 cactaaatac gctgaccatt tatcctatgg tcagcttttt atgtaaaaga agatactact
    52921 tctaacacat tgtttatctg taaaaaagag cgtgggacaa aagtaaagaa cacttttgtc
    52981 tcacgctcta aacacgtata aacggcggaa gcagaagcaa ctccttcgga aataagctga
    53041 aattcacaaa aatttaaaaa gcaattttct tgagtttctt cttatttctt ggagttaaac
    53101 gcttctgtcc cagcctcttt tttccttcct tcaatttttt acacgtcgac gattcaataa
    53161 aaaaccgcct aatattgtaa agacaggtgc aagaatcgag aaaaaggcga atggcaagta
    53221 atgcaacgcg ctaactttca tcgttcccat aatgaatgtg ccgctgactc cccacggtac
    53281 gagagaattg acggtcgcac ctgaatccgc tagtgttcgt gtcaaatagg tttttgataa
    53341 gccaacgtca tcaaaagaac gcttgaatgt ttctcctgga agaatgatgg acagatactg
    53401 ttctccaacg atcagattga ttccgataca gctcaaggca gtaaacccga ctaaacgaga
    53461 aggtcggtcc atcttttctt ttaattcttg aacgatggta tcaacgatca gatatttgat
    53521 cagtaaccca cctaatgcca atgcaagtaa aatcaaacta gcagaaccta acatactcat
    53581 gatcccgcct cgagaaagca gttcatctag ctttggatca ggtgtatttg cgacataacc
    53641 attcatcaag atgtctgctg cttttccgac cgtcaaactt ttctcatgga taaaagaaag
    53701 aacgagagca gctgtcgatc ccgctagtaa tgtagggata gccggaattt ttttgattgc
    53761 ccccccaaat aaaataacta gtggaatcaa tgtccaagga gagatccaaa agctcgcttt
    53821 catgttttgg atcatttcat tgatactagc tagatcggct gaatgactgt tatgtcctaa
    53881 tatcgtataa aaaatcaacg aaaggacgaa tgctgggaaa tccgtataac gcataccagc
    53941 gatatgttcg aatagattaa ctcctccaat acccgctgcc aagttggtcg tatctgataa
    54001 aggcgagcag ttattcccta aaaaggcacc ggaaacgact gcgcctgtag tcattgcatc
    54061 attgaatcca agtaaatgac cgatccccaa aaaagcaatt ccaatcgtcg aaatcgttgt
    54121 aaatgaactg ccgacagtca ctccgacaat tgcacaaaca agaaatgctg tcggtaaaaa
    54181 gaaacgcaca gaaacaagtt taaatccata aaccataatc gtagggatgg ttccagataa
    54241 aatccatgaa gcaactaaga caccaatcaa aagaaaaatc aggattggaa tgatccctgg
    54301 agtgattcct ttgatgatcc cgtcatggat ctcatcccat gtgaactttt taaatctgcc
    54361 ataaaacaac aaacacatga aagctgcaag gatcgggata tgtggcgaga gctggaatcc
    54421 gatgatcagt actcctagga gtgccaacaa cacgatcaat acagcgatac tttcttttaa
    54481 agaaatattt ttattcatcg tcgatctcct atttcaaatg aaaaggccct gccatgaaaa
    54541 aactattcac ttggcaggac ctcctactaa atcagcgaca aaacgtcggc tgaaagactt
    54601 atttttttat ttttcttcta aacttttttc tacctcaatc ccccaagaat ctttcttgaa
    54661 gtgattgatg atcaatggaa taacgaacat gaccactgtg atagctgcta ctaataatgc
    54721 atcactcaat gtcgctgtac ctaatgtagc aaatacgaca acgatgatag agaagatcag
    54781 catgcagctg actacccaag ctaatccgtt tccgttttta ccaattcggt aaggacgttc
    54841 gaattctgga tgtttcttac gcattttaag caatgaaaca gcaatcaaaa tgtacacgat
    54901 acaataaata acagttgtag catttgtaag tgtcaagaat acagcactta cgtcatccat
    54961 tacaccataa agcaaagcaa ataatgaaat aacgattgat tgtgttagaa cgacatttct
    55021 agatacgccg tatttgtttt ctttatggaa tccgaatttt ggtggaagga atccttcacg
    55081 agctacttgg atgattgttt ttgatggtcc agtaacccaa gctgataatt gtaaaagcac
    55141 accaataaat accatgaagc tgaagatatt tccgatcact tctggtaagc ctaggatttg
    55201 acagtcaatc aagataggct gcgtgatatt tgctaattcc atttttccat ctggtacgac
    55261 atttgctaca agcatcgcat tgatgacatt caataaaact aatccgatca aagcgataaa
    55321 tacgcctttt gtataatttt ttgttgcgtc tttcaaacga ggcatgtaaa cagaactcat
    55381 ttcgatccca acaaaaataa atgtgatacc agctaagtat ttcaatgaat caatatgttc
    55441 caagtcaggg aaagcttttg accatgaaaa gtcacctaga tagccgtttg agactagtcc
    55501 gactttgaag gctgccaaaa cacctaaaac gaacatgatg actactggga tatatacccc
    55561 tagccataca ccgatgttcc cgccgacttt tgccatatcg aatttcaagt tcaagatcgt
    55621 gatgatccag tagataacta aaatacaacc taaaacgaac cagtgattat tgccaagttc
    55681 tacattacca aatgtgtttc ctaacaatgg tcctaaagta gaagcgacca ttaccatacc
    55741 tgggaacatt tgcacccata atagccatgc aacgacaaat ccccatttgc tgcccagtgc
    55801 tgtttttacc cataactgag gaccgccttc ttgaggaagc atcgtggaaa gctcaccgga
    55861 aaccattgat atcggaccgg caaaaaacaa aactgcaaaa attgaataaa aaatcaatgt
    55921 ccaaccgact gcggctaacg taggaatact ccgaaccgtt gcacacagtg ccattgtcag
    55981 accgataaag gtcccaagac ccattttact tacttcagat ttactcaccg ttgacactct
    56041 ccttgatttt ttggataaaa taacggtgtc tttgaagtag tttcagcaat aaacgaaaca
    56101 attcagcgaa tatttgaggg gattctttga gtttattgct tgaagggttt cttcaaagac
    56161 aacctcaaaa caaagggatc gaagagctta ggtctcccct tgctcttcag atccaaacat
    56221 gcgtcagaaa cagttagtat tcaagctgtt ttctaacatg attaccttgc ttattttgct
    56281 tcgcttgcca ataattggtc tgcgtagatt ttttccaatt tttcttgaag agctgctttg
    56341 attttttcag catattcttc aaagtgttct tctttgttca tgtatggtgt cattacgctg
    56401 gcacgcaata cagttacttt tccggcacgg ttccattctt catctgagaa tcctagttgg
    56461 ttcacaaatt gtaatgggct gttgccatag tctgggatag caaagtcagt atgtgaagtt
    56521 aagaactcat taccgtaaat gcttcctttc acgtatgaag aataatcata tacgtcatgg
    56581 ttcaatttat tcattgcaac taagtcatcg ttgccttttt ctttgaatac atagtctacc
    56641 atattgaaat ctggataagt taatggatga acttcgattt ctttgtctcc tactttaaat
    56701 gacaagtcat tcaagaagtt gtagaaacga tgtgcacctt cgattgatgc acccattaat
    56761 ttaccatatc ctgtgacatt caaaggtaag acatggtgtg ctgcccaaac gctggctgct
    56821 gttgcccctg cttttgaacc ttccaagata taagccccaa gtaatgctgg gatatctgcg
    56881 cctttttcaa atacatatgt cgcaaagtaa gagataacgt ctctcatgcg gatatctttg
    56941 atgacgatac caccggcaga atatggaaca tagcccattt tatgtgggtc aatcgttaca
    57001 gattctgctt cttcgattgc tttatatgca ctgtgtactt cttctaagat ataatctttg
    57061 ttttctgtaa agacattgta tttgtaatga acatctttta aatcttcaaa tggaatgaag
    57121 ttgttgtctt cgtctaagaa aatcgcacgg ccgtatccgc cataagcagc atctacgtgt
    57181 aagtagaagt aaataccgtc tttttctaat acgcgacgca atgcaacgat tttatcgata
    57241 ccgtcgattg ctccttcttc tgttgatcct acaacaccga ctacacctag gattggtgtt
    57301 ttttcagctg ctagaccacg aacgattttt tcaagttcgt tgatgtccat gcgatagttg
    57361 tggtcaactg gcactggaat cacttggtct aaaccgatac cgatgatgtc ggcagctttt
    57421 agccatgaat agtgtttagt ttgtggaacc aaccattttc ctaatttttc taaatgtttt
    57481 ccgctacgtg ctgagtgagc tttgatttcg tcgatttttt ctgggacgct gtctaacaga
    57541 ttcatgattt cttctgttga caagttcatt aattcccaat cgctttttcc agcaactagt
    57601 tcaggtgtca cttctttcat tgcaagcggc aatgatttga tgttgcgggc ataccaaagt
    57661 ccttctaagt ttgctaaaga accatcagca acgatgtggc cccagccatc tttatagctc
    57721 attaattttg caaattccat tcctacttct tcttccattt ggcttgttgc aggagaagat
    57781 tcataggcaa cgttattacc attccatagc atagcaaaat tgtacgctag taaagatggc
    57841 atcaatgttt cagagttcat atgaccccag taacgtccgg cattatgcca aggaacggaa
    57901 tgggtacgca tccgcgctga aatttctgat agaacatcct ttgttttatt cacagtgtgt
    57961 tcaaaactag cactgctttt ttcttctggc gtaataatag gcatgtcttg aggcatgtag
    58021 ttttgacgcc agcctaaatg ttcatccacc aattcattca acaaagcttt atagatttgg
    58081 ccgttctcag ctttgtcccc aatgaatagg gcatttaggt ttagatcttt cgacaatgat
    58141 tcactcatag tgaaaccctc ctaaaaaaat atttaaattt tgtcgagccc tacattacac
    58201 ctgttcttct attctgtaaa ctaagttttg gcctgatttt taatcatatt ttaatatgta
    58261 tcaattatcc gaattgttca cacttttgaa ataattaaaa aaaacgaggg aatttacaaa
    58321 tgtaaactcc ctcgcaaaga ttgtattaac gacaaccagc gttctatttt aggatgggtt
    58381 tcaaaattgg aatgttgtaa tagtatttcc ctttttcaag gtaaaaactt actaaattct
    58441 aatcaattac tttggccaaa aagtaatttt tcttaccttt tcgaataacc acaaacttac
    58501 catcgaattc atcggatgga tttatgacaa aatttaaatc ggtaacgcgg tcaccgttaa
    58561 tacttatagc tccgtttgaa acatcttcac gtgcttgacg tttagatggt tcaatttttg
    58621 tggaaacaag cagttccacg atattttcag gtgtgcttga aatttcaacg ttcggcattt
    58681 taccgaatcc ttgcgcgatt tcttccgcat ttagttcttt aatatttcca gagaacaatg
    58741 cttgcgttat tttttgcgct tctttcaagt cttcttcact atgcacaaat cttgtaactt
    58801 cttctgccaa acggcgttgt gcttcacgtt tctcaggttc tgtctctact tttttcgcta
    58861 agtcttcgat ttcttcttga gacaagaaag taaagaattt caagtatttc actacatcgc
    58921 gatcatcttg attcaaccag aattggtaga actcaaatgg tgaagttttc ttcggatcaa
    58981 gccaaacggc accgcctgca gtttttccga atttcgttcc gtcagccttc agcatcaatg
    59041 ggatcgttaa accgaacgct tctgcttctg gtccttctaa ttttcggatt aggtctaatc
    59101 cagctgtgat atttccccat tggtctgctc caccgatttg caattgcaca tcgtatgttt
    59161 tatgcagatg caaaaagtcg atagattgca ggatttggta agtaaactct gtaaatgaga
    59221 ttcctacttc caaacggctg gcaacgatat cttttgctaa catcgtgttg atgttgaagt
    59281 ttttaccgta gtctctcaaa aattcaagta aagagatttt tgacaaccag tcgtagttgt
    59341 tcacaaatgt gatatttgca tctttaccaa acaattttct catttgattg gaaagtgcgc
    59401 ccacgttatg ttgcacttgt tccatcgttt gcaacacacg ttctgttttg cggccgcttg
    59461 ggtcgccgat cgttcctgtt ccgccgccaa tcagaatata tggatgatgg cctgccaatt
    59521 ggaatctttt catcatcata aatggaatca aatgtccgat atgcatcgaa tcaccagttg
    59581 gatcaacgcc gcaataaagt gaaatgctct tttcttcaac gagctcgcga agtctttctt
    59641 cgtttgtttg ctgattaatg gcgtcgcgcc aagttaattc atcaataata ttcattttac
    59701 tgacctccgt gtagtttttt tatgaaagca ggaacctgct agcatgtgta attaattatt
    59761 tcttcaggat aggataggaa ataaaaaaag tcccttggca tataagccaa gggacgaaat
    59821 ctcgcggtac cacccaattt gtatgtctag atatccaaca tacctctcga acatttaacg
    59881 ctgtcgcgtg gcaatcactg ccaaagactc ctgaaatgta attcgcttta aagcatgtac
    59941 tggtttgcag cgaccaccag ctttctgaaa cattggcttt aacaactact tcgtttcgat
    60001 cttcgtcttg ttattattaa tatgtgtcta tcatagagcg caactttttc tttgtcaaca
    60061 gaaaaaggat atttttttta aattcctcat tttttctttt ttgaatccgc tctaattccg
    60121 aacattttat tttcaattat tgttaaaaga aataactttt tctgtatttt ttctgcaatt
    60181 tatcgagaag tcactttact ttgcctctta ttctttcttc gttacaataa ggaatgggac
    60241 aagtaaaaaa atgaaaaagc aagggacgag gtatatggaa aaaaagcaaa aaattatttt
    60301 aggagccact tctgctttaa ctatcgttat tttaattcta agtttgtttt ttgtcatccg
    60361 atcatctaca aaaaaagaac caatcattcc tgaaaaaaaa ataacagaaa aagcaaaaga
    60421 accgacagat gataagcaac aactagttac tcaagcaaaa aatctggctc aaagctacga
    60481 ttatgaccaa gcgattgcgt tattggaaaa agatagtaag aacggatcta ctgaaacaca
    60541 acagcttctt caaacactaa aaaaggagaa agaacaactg atcgactggt ctgatcctac
    60601 tcagatctct catgtctttt tccacagctt gatcgtggac ccgcaaaaag ccttccactc
    60661 tcaacaggca caaggataca aagattatat ggtgactgtt gaagaattca accgttcgat
    60721 cgaacaactt tatcaaaatg attttgtttt agttaatttg aatgatttga tccaaaaaga
    60781 cgaacaagga aatctttcct ttaacgggct tgcgctacct gaaggaaaaa aaccattggt
    60841 tctttcacaa gatgatgtca gctattatga atatatggat aattccggtt ttcctagccg
    60901 attggtcgta tcaaataaca atgaaatcaa aaatgtctat aaagagaaag ataaagaaga
    60961 aatcggtgac tttgacatgg ttcctctgat cgatgccttt gtcaagaaac atcctgattt
    61021 ttcttatcga ggcgcaaaag gtaccttggc cctgacaggt tacaacggag ttttaggtta
    61081 tcgaacatca aaatctcaat atggagacaa tgaaaaaaca aaaaaagaga ttaaagaagc
    61141 taaaaaagtg gcggaacagt tgaagaaaga cggctggagc tttgcttccc atacatgggg
    61201 tcacttgaat atgactcaag cttcactaga agatgtaaaa aaagacaatg aattgtggca
    61261 gcaagaagtt gcacctatcc taggaaaaac aaatattctg atttatccat ttggtgcaga
    61321 catcagcgac tggcagcctt attcagcaga aaatcaaaaa tttgcttact tgaagagtca
    61381 aggtttcgat attttctgta atgtcgatgc ttctacacca gcttggggac aattaggtac
    61441 ggatttctat cgaaatgctc ggatcaatat cgatggcatt cgttttcaat ctgatttaga
    61501 cggaaaaaat cctattcttg atccattcat caatgtccat gatgtctatg acaaagaagc
    61561 acgcgaatct gcttgatttt acgtcattta tgaaccaaaa aagccgaact gacattttat
    61621 cgtcagtttc ggctttttta tgctttattt cttcttcggt gcgattccct tgatcaggac
    61681 ttctgtccat tctgctgtat atggaagcag ttttgcgtat atctccggct tagaaattcc
    61741 ttctaccatt gcttgaaaat agaggatcaa gaactcatcc gaatatttca aatccacttt
    61801 tccttctttc cgccctcgat ggaaaagatc aagcatgata ctgaaacttt cttgcgtata
    61861 cgcatccatc gcgtcagaaa attctttttt ttcttttttt gtatagtatg tcatcaaatc
    61921 gatataaaat tgtttgttca ctcgattgag ataacggatt ttgttttcac tcatggcaat
    61981 cagtgtttct tcaaatggaa gattttccgc aatcaacgct ttcgcttctt gccctaaatc
    62041 attcataaat gcaaaaaata cttcataaat cagcccttcc ttgctgccaa aatatttaaa
    62101 aatcgtcgct ttacttacct gagcgttctc agctacctta tccattgtca ctccagcggt
    62161 tcctatgtct gtgttcatca agtcaaaagc tgcttgtaaa atctctttct ttttctcttg
    62221 tgctctcttt tcaaaaccgt tcatttgtag agcctcccgt aattttaaac ttttataata
    62281 tatatagttc aaatttattc tgaggtttat tataactatc tattgagaaa gtatcaagct
    62341 agcaattcct tgtttatgaa ttcattgaca gaaatttata aaaagaatat tatgtactaa
    62401 aataataaaa aaagttttat aaaaaggtat aaaacctttt tattaaacta tttaatcttg
    62461 tttagtatat atttttagaa aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac
    62521 acatttgaaa aagagctttg gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca
    62581 ttcaggtgaa gtcgttggat ttatcggacc aaatggttct ggaaaatcca cgacgattcg
    62641 gacactttta gggattataa aaaaagacgg cggaacggca acgatttttg ggcaagatgt
    62701 atgggaagat agtttatcta tccatgaacg tctttcttat gttccaggcg atatctcttt
    62761 gtggggaaat ttgactggag gagcgatcat cgatctcttc atgaagcttc atggaaaagg
    62821 gagtgctcaa aaaaaagatg aactgattca acgtttctct cttgatccta agaaaaaagc
    62881 aaaaaactat tcaaaaggaa atcgccaaaa agtgggcttg attgctgctt tggctgttga
    62941 ttcggatctc tatttatttg atgagcctac ttctggttta gatcctttga tggaaacgat
    63001 tttccaagaa gaagttgaaa aattgaaaga acagggcaaa tccatcctac tttcttctca
    63061 tattctcagt gaagtcgagc gactagcgga taaagtcgtg atcatacgcg aaggaacact
    63121 cgatgaagaa ggaacactcg atgaattgcg tcatttgact cgttcgacca tcactataga
    63181 aacagaagca gagaatagtc acttagcaca attgaacggt gtccatgact ttgtgcaaaa
    63241 aggtcacctc gctcaatttg ctgctgataa cgaagctttc gatgtgattt tagctgaagc
    63301 gtccaaatta ggtgtgaaaa aatttgaatc tattccaccg actcttgagg atttattcat
    63361 gagacattat gaaggatgag gaggaagcga aatgactgat aaattttcac gctggtccac
    63421 ccttttttct caaaacctta gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt
    63481 cactgctttt tctggtggtt ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt
    63541 agctggatta tacgaaacct tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc
    63601 agcttcgaat gctgctgagt atactttagg agcgatgtat gcaaatgaaa tgttgctttt
    63661 ttgcagtttg tttgctgctg tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa
    63721 agaagaactt ggtctgattg aacttattcg ttcctttcga gtcggacgtc aagcaaactc
    63781 tttagctgtc atcgctgaaa gtattctgat caatttgctt ttagcaatat cgatcagttg
    63841 tacgatgatt tttttccaag cggattcgat cactgcagaa ggtgcttggc tttttgggct
    63901 atctgtaggt agtatgggga ttttggggag tgccgttgca ctgtttgctg cacaactgat
    63961 gccaagttca gccagttcga atggtctgtc tttagcgttt ttaggtggac tttaccttct
    64021 acgagcaata acagatgtag ctgattcctc cctttcgatg tggaatcctt taggctggtc
    64081 ttatttgacc tatccattta caactaacaa ctggattcct ttgctttatt tgtttttgtt
    64141 cagtctaatc ttgataatca gttcatttgt tttagaggaa catcgagatt tgaacaccgg
    64201 ctatatcccc gaacgtgaag gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg
    64261 tttattattc tatctaaata aagggatgac aatcagttgg cttatcactt ttggtttgat
    64321 gggggcggca tacggctcta tctacgggaa tatgcaacag tttttagaaa gtaacgactt
    64381 aatgaaacag atgtttatcc aatctggcgt ctcaattgaa aaaaacttta caagcacgat
    64441 catggtcgtc ttgatttgtc tagctgctat tttaccaatt gctagcatca atcgtttatt
    64501 tacagaagaa acccacggac gtatgaatca gctgttcgca acaaaaattt ctaggaacca
    64561 actttattgg accaatatgt tactagctat tctatttgct atattggcaa tcctgattag
    64621 ttctggtgca ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag acatggatct
    64681 attgatgttt cttaaagccg ggttcaattt cctgcctgcc gttttagtgt ttatcggaat
    64741 tagtggcttt ctattcggat ggcttcctcg aatcggtaaa cttgcttata tctacttagc
    64801 ttactccttt gcactcaatt actttggcgg aattttggat ttaccagaat ggttttccaa
    64861 aacagctgtc cctagctggg ttccacggat gccaaacgat tcttttgatc tttccgtgtt
    64921 ccttacgtta tcatgtatca gccttgcact gttcgtcctt ggttctattg gttataggcg
    64981 aagagactta ctagaaggat aataaaaaag catgtacaat ttcatgctag cctccaaatc
    65041 tgggtcaaaa atccaacatt tggaggtcat ttttttattt acaagtcttc taactgctct
    65101 ttatcaatga tccagacttc tcgattcttt ctttttacat aaccattctc ctctaataat
    65161 ttaaattttc tagataaagt ttcgggggtc gtaccgataa atgcagcaag ttctttcatt
    65221 ttcattggca ggaaaaagtg atccttccct gtcgctttag ctaagtcaag cagatagaca
    65281 gcgactcgtt cttctacttt ttccatcatc aaaaaagttg tctgctgctg tacttttgtt
    65341 aatttctcaa cgttgattgc taaaagtttc agactaaggg cgggataatg cagtaagagt
    65401 tccgagaaat ctttttgtct caatatgcaa atttccgttt tttctagtgc ttcgccaaat
    65461 aaagactcat ttggaacgcc tagcaactgg ctctctccct catatccgcc cggctctatt
    65521 actcgtaaaa gctgttcctt tccgctagca gataattgat acactttcat actgccggag
    65581 gccacgatca ctaattgagg ttctccattc ggcgcaagaa tctgttctcc tttttccaca
    65641 tagtgatgtt tgacaagtgc gtgtactttt tcttgatctt gctgtactaa gtgattgaac
    65701 aaaggaacaa atgaaacaca taaatgatca tgactcataa tctctcctcc ttctaaacag
    65761 ctatttctaa taaagaaaca taacttccct tattccaact gataattttc ttttatcata
    65821 gcataaacgt caagcgaacc agataccagc aaatagagtg gatttctttt tgtcccttgc
    65881 tttattttcc ttcttttgag accttaagta attgattcct atcaattttt taaatattta
    65941 ctttttgaat aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg
    66001 cattgcttat ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg atatagtggt
    66061 gctagttctt gtaaggtttc agcggttatt tgctcaacgt gttcgatttc ttgcggatag
    66121 aagaatagtt tacccatttg aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt
    66181 ttctttgtat cggacaaata gtcagacaaa gctaagtggt cgtcaagttc atgttctggt
    66241 tgcggccata tgtaataatc cttgatatcg actgttttgc tccactcttt cagtatcgca
    66301 agcaactggt tatacgctgc aatgctacca tatcgttctt cgcttttata atgttggtac
    66361 atcaaataga tttggtattg tttttgattc aacaaaactg ctagacaggc attctcgtct
    66421 tgacgagatt cgcttcggta cgcgctccaa aaatgactgc gtaaattcca gccattcgtc
    66481 cagctttcga ttttaggttt gctcatgccg tacatatcag gcaaaagtgc agctgtctgg
    66541 agctgaatct ctttccattt ttgccagtgt tctttatatt ctttcttgat ttgttcaatc
    66601 ttatctgttg gatggctctc tttgagctgt ttgaatgcgt agattggtcg atcaaacaac
    66661 tgataatctg attttccaag ttccattgtt tcgcctcttt ccgttatctt ttgtattcta
    66721 cctgaatttt gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat
    66781 aagaacagcc aaacaaaaaa ctgcgatgag catcaccgca gttttattga acttaaggtt
    66841 gattgatcac atctaaaagc gttgaaaata aaatggcact agaagcagct cctgggtcga
    66901 tatgtccgat tgcacgctct cctagatagg aagctcgtcc ttttttagct accaagactt
    66961 tggtgtgttc ttttgcctgg tcaatgattt cttttgttaa attccctgcc ttcaatgcct
    67021 ctgtgactgg cagccaaaca tctaacatcg ttttatcgtc agcttctgcc tttcctcggc
    67081 tttgaatacc cgctgttccc tgctcgatca cttcgcctaa tttttcttga gaatcaatag
    67141 aatcgacctc ttttgtcgct ttcatcatgt tcatgaatgc agatccgtat aacggaccag
    67201 aagccccacc gactttcgat atcatcgcca tagagaatac tttgaatgta tcgctgattg
    67261 tttcaggatg atcggcttga aaagcttctt cataagctga aacccctctt cccatgttat
    67321 ttccatggtc tccatcgcca atcggggtat ctagatcact gagatattgt ttgttctgat
    67381 tgattgcctc tgcaaattgc gtgagccatt cttggatttc ttgaacggtt agtttcatat
    67441 tatcaccctt ttatcttgag attttttacc atgaaatcgt ctgtacattg ctttctaaag
    67501 aatctttcca ttgtgtctcc gtcaagtcga tcagtgttag agaaagacct tgcatatcta
    67561 gtgaggtcat aaaattaccg acttttcgga aaactacatt gacgcctctt tcttccaaca
    67621 gagctaaaac atcattcatg aacacgaact gttccatcaa aggcgtcccg cccattccgt
    67681 tgactagtac acctgcttct tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa
    67741 gttcctttgc cagtacttta gaaggctgca tcttttctcg acgatagcct ggctcaccgt
    67801 ggataccaac accatactcg atctcatctt ctcctagttc gaatcctggt ttccctactt
    67861 ctggtacagt tgctgctcga agcgctagac cgatcgtctt agttgcctgt acgacctttt
    67921 ctcccagttc tttcaactca gctagggaag caccgtttct tgctgcatcg cccacgattt
    67981 tgtgtacaag gacagtacct gcaactccgc gttttccagc agtatacgta ctattttcaa
    68041 cggcgatatc atcatctacg atcacacttt ctacttcaat atcgtccata gcagctaatt
    68101 cctttgccat atcgaagttc aatgcatccc ctgtataatt cttaacgatc agcaaaactc
    68161 cttttccact atcggcttcc ttaatagccg tctgaatctg atctggagtt ggagaagtaa
    68221 acacatcacc aagcaccgct gcacttaaca tcccttcccc tacaaacccg gcatgggatg
    68281 gttcatgtcc gcttcctcca ccagaaacca aaccaacttg ttccgctttc tgattcttgg
    68341 ctacaacacg ggaagattct acacgatgaa caagttctgg atagcttttt actaatcctt
    68401 ccagcatttc ttcgacaata ttgcttggat cattcatgat ttttttcaat ttattcccat
    68461 cctttctttc cggcattttg tgcttctgtt aagatttgct gagtatcatc tgttataccg
    68521 gcagtgatgg ctgctgcaaa agctccttcc actaaaggcg catccaccag atgataacgt
    68581 tgctgctgct cttcttccaa catgtctttt gccagttccg agttcaatac cgcgctaccg
    68641 atatccgcaa aaacaaaata actagctgca tcgcttgatt cattgactgc ttctacgatt
    68701 ttcatcgggt ctgatccgat ctcgccattt attccgccga gagaaaaaat ttgcacctct
    68761 tcggattttt gcatctgctc aatcatttct ttaataccat ctgttacttt ttgactatga
    68821 gaaataagca aaatactttt tttcacaaga atcttccttt cttttacata gttgctaacg
    68881 attggtttcg ctttcattaa tgaacaattc aagtatataa taaatttcta attttgggaa
    68941 aatatgtgta gtgcttcttg aagtttgtta tactttaatt aaaagagagg agtgatcgaa
    69001 tgattacaac aacaacgaac agtgtagagg gaaaagccgt agtagcttat aaagggatcg
    69061 tttttggtga ggtcattact gggatccatg catttaaaga cttggaggcg gggttgcgaa
    69121 atatctttgg cggccgctca aaaagctatg aaaacgaact aatgggtgca agagaagaag
    69181 cacttgccga aatggccgaa cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa
    69241 aaatggacta tgaagtatta ggcagcgaca atggcatgct gatggtcaca tgtagtggaa
    69301 cagctgtctc cacgagataa gtaggggaca tctgttttaa taactaaaga gagatgggac
    69361 atgtagtggc actcaagaat tagatttttc agtctaactt cgaggtactc taccttccca
    69421 tcctctcttt tttatttctt tttgcggatg attccgtaag ctgctgcgac taacacaccc
    69481 gcccctgcta aggaataact gacagaagct gttttttcat ttgtttttgg caaagtctga
    69541 cttgttgatt tggatggctc tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc
    69601 actgattttt ctcctgccga atgcaaaata tcttttattc gttggaaact agctgctaag
    69661 tcagcttttt ttgcttcaaa atcattagca actgtttgtg cttcgctgat ctttccgtgg
    69721 attgcttgac tggccgctgt gatatccgct tgagtttgac ttagctttcc agaagctgtt
    69781 tgagcaacct gatttaattg ctgattgaat tcttttgctt gttggatctt cccgtcaaga
    69841 tctgtttgga cagcttccgc ttttgctttc gcttcggcaa gctttttctc aagttgtgca
    69901 cggtcatttc ccactttatt tttaagatca gctaaatctt gatcgattgc tgcttggtct
    69961 tctttcaatt tgttttttgc agcttctaat gcttcttcga gttcttttct tttattttcc
    70021 aagtcttgca aatgatcgat ttggtcagaa gaggtagaac tatttattga tgtatcttct
    70081 gaagatgaaa tcgtttctcc tgaattatct gtcggtacat tggaagtcat ttctgtttcc
    70141 gaggcaccgt tttcagcacc agaagagctt atggttgttg aaggttctgt ttgagcaggt
    70201 tcagaagttt ccgcaaaagc tgtctgtccc aataagaaaa tactggaaac agcaattggc
    70261 cagattaacg gcttcattct ttttttcatc acttttccca tccttactac tttttaacta
    70321 atttcttatt ataagcaaaa atgtttggaa gaaccataaa cacaacaggc tgtttgtcta
    70381 aaaaacggtg catcttttat ttttttgtac gttccgggct tttccatgtc ccgcttccat
    70441 aacctaaaat cgttttgact gctggaatga aagtcgtcac gatcgttatc gcgttcacta
    70501 tccagaacaa aagcatatat agtggtgcga aaagaaggta tttcaatttt cttcgtcgat
    70561 catctacaat cagcgaagct agcaactgga aaaatcctgc gaccatttca aagcagatga
    70621 acacaaaact gaatgtaaaa gtaatataaa tctgttcgta gtttccttga tatacataat
    70681 gcccgattaa tccagcaaaa agaactacgg aaatacaaaa gaatattgac cagacaatac
    70741 tcaatgtttg atcaatgaag atgatcgttc ttccaatatg tttaaaagga tggagcatca
    70801 cttttttgaa attcgtcagc cagacttctg ttccaccttt tgcccaacgt tttctgtgtc
    70861 gatatagcat cttcaacgtc acaggtactt ccatgaaaaa gataatctca ggagcaaaaa
    70921 cagataacca gtcatttaac tgatgatccc aagcaatact gatgtcttct gtcgctcgat
    70981 cttgtctgaa tccgcccaca tcgatcaagg cttcttttcg atacatcgta tttgctccac
    71041 tgtacgcata aagaccaccg aaaacagctg actgtgttcg tttaatgatg cccacgatac
    71101 ttgagaattc aaccgtttga gattttgcaa tcaatttggt acgattttgg acatccatat
    71161 ttgcagtgac cgcagcaata tgtcttgcac cagggcgaat gaaataattc acatacctaa
    71221 tcagtgcatc tggctccggt accgtgtctg catcattgct taaaatcaat ttcccttttg
    71281 caaaggcaag tccgatatta aacgcgtggg cttttccttt atttttttcg atccgaacca
    71341 cacgaagatt agcgtatttt ttcatcagtc tagctaatat ttctggtgtt tggtccgttg
    71401 aaccatcgtc tgtcactaat acctcatagt tgtgataatt caatttcgtc atcaaatact
    71461 cgattgtata ttcgatcaca atttcttcat tatgggctgg caccatgatc gtgatgaacg
    71521 gttcgacact caagggaaca tctacccatt ctttttgctt atgtttgtat aaaaacacat
    71581 aacaccagac accaataaac catacaaatc cgcctaaaat cggataagaa acgagaaaaa
    71641 aatacataat agacaaacaa gtatctaaga ataaattacc tgtatgggtg attgagatca
    71701 tttcagttcc accccatttt ctttatacaa atctgcaatg aatgtcgtat ctagattttt
    71761 ctcttcggag acagaataaa aacggaccgt ttctctctct tcttttgttc caaaacgttc
    71821 cgtatacact tcttcaagta attcttgtct ttttttcaat ttttcatctt catattcaaa
    71881 agatttattt acttttttgt atagatttcg attgttccac ctagtcaata caaatgaggt
    71941 aatcaggaaa ctgaaaaaaa tcaatacaaa aaagccgatt aagaaataca gcattttctt
    72001 ttctgataaa aaagcgtaca aggaaaaatc cttggctctt tctggtctcg ttaatgggaa
    72061 aaagacccat ataaaaggaa gaacaacgat catccatcct aaaagagcca ctaacgtttg
    72121 ccaaatcttt aaccagtaat taccttttga aaaatactta tctttcacca aaattgatgc
    72181 aacttcttct cttgttactt ttttcatatc ctcacctcac gtttttttgt tacgacgtag
    72241 gagtttacca tacatttcat ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa
    72301 aagaacaaaa catttctcat cagcaaatga aaaatatttt gttcttttta tcagttgttc
    72361 tttttatttt ttatctctta attatgttgc tctttccaat acgacaatgc ttttcattcc
    72421 tctagatatc gccgtgtaca tttgtctgcg tcttctttgc tcatctccat cgttttcaaa
    72481 tggatataag attacatgat caaattccag tccttttgct agactcaaag gaatgatttg
    72541 gcagttttct aatccatatg aatttttttt aatcgattct tgtagcgttt gggtttcctc
    72601 ttctgtttgg gtgataactg ccgctttttc tccttttaga gaaaccaaaa tctcagctag
    72661 tattttcaac aattcttcag aggatcgaaa agagcggtat tcgggttctt ttccttgcgg
    72721 tcggatcgat acgatgtcga tcttctcttg atccacagca taggttttga aaagctctgt
    72781 gattgctccg ctagaacggt aactatttct aagatcatac cgatggatcg gcagatggta
    72841 gtcgtcgaaa caacgcataa tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt
    72901 ctcgtcacct actaaagtaa agtgagtttt tggaaataat tcgatcaata gacctaattg
    72961 tgccagggta taatcttgta cttcatcaat caatagatag cgaaatgctg gtacatttac
    73021 cttttcaaca agtaaatgac gaatagctaa tataatgact gcttcatcaa cagtacgtgt
    73081 gggttgccaa gcataagctg agccatatcg tttttcataa attctttcaa acaactgatg
    73141 ctcatccacc catgccattt cttcgatttg tctggttatt tttcgatatt ttttttgtaa
    73201 aagtttcctt gcataagcta caatactttg ttccgaatca tcagaaatca atttaccgaa
    73261 taattccaac tgcctatctt ctgtcaacga gagtacctga ttctgcacag caggactttt
    73321 tgcttgtttc aaaagatgat tcttccattc gctcatcagt gcctttttcg ttgcttgaag
    73381 cctatcgatc aatttaggat gagcaggaac tttctcatat attttttcga tcgttttttt
    73441 agaaaggact gtttttccat ttttagtaag tccacggata aaattcggat gatctaaaaa
    73501 caaaggatct gattgtttta gattatcgat aaactttttg ctacgcaaac gttctgtctg
    73561 ctccgataca ttttctcccg tgatacgttt gaaataagcc tcttcacctt ctatttcctc
    73621 tgctgaaagc tgtgaaacca gctgtatgat ggttagattc agtggatttc tttcacctaa
    73681 tgatggtagg acattcgaaa tatagtcgat aaaccgatga ttaggtgata gaatcagcaa
    73741 gtcatcggat gtcatcgtat ctcgatataa atagagcaga tacgcaatac gctgcatgat
    73801 agcagaggtt ttaccacttc ctgctacgcc atttaccaag atattttgac ttttcacatc
    73861 ccgaatgatt gtgttttgtt ccgattggat cgtagcagtg atagccttca tttcattggt
    73921 ctcatcttgg cctaagactt ctaataatac gtcatcttga atagctacag acgtatcaaa
    73981 aaacttgacc aaacgatctc tttccaaaag aaattgtcgt ctttctttaa taaccgtatc
    74041 gattttattt tgacgaaccg tataagaaga tggaccaatc atgttattgt aaaaaagttc
    74101 ggcaattggt gaacgccaat cataaacaag attttcatct gcatcatctg tgaaatttgc
    74161 tgtaccgata taaaaggatt cttttggctc attatccaga aagtctacaa cgacttttcc
    74221 aaagtaggga cttttcaata aacgttcgat ctttgctagc tgattttctg cgtttttctg
    74281 tttgatattc atctgatcga tttctctatt tttcatttca atcatcgaaa aagtatccag
    74341 gttatctaag taactgtcaa aattcaatcg aagctcgcca gccatttctt tcaacgactg
    74401 gtttccttct atttttgccg tatccatcga ttcccgcagt tcgttttttt tatcaattag
    74461 cttttgatac acgttcttca gatatgcttc ttctaactgt ttttgtgtgt tgaccataca
    74521 cattctcctc tttttttaaa gtggtggaac cgattagttt acctctttta agagtcgaat
    74581 gcaagctttt taacgagtat catttgttag gatatatcag ttcttcaggt cggacagttt
    74641 gatataaaga aaatgcacca tcaaggtttt ttacgtggta gccagactgc ttcaagattc
    74701 tttccgctag atagctacgt aatccgctat gacagctgac gatatattct tgttggctgt
    74761 ctaattcatt taatcggtcc cgaagttcat tcaaagggat atgtttggct tccttgaatt
    74821 tgcccttttc caattcttct gctgttcgca catctaataa aatttttcct tttgctagtt
    74881 ctgtcggcaa ttcatgccat tgtacggttt cactaagccc ttctgcaatg ttcattgctg
    74941 catatcctag catgtttact ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt
    75001 ctaattccgg taaatcgaag atcgtcaaat gccctttgat tgctgttgct aaaatatcga
    75061 tccgtttatc cacacctttt gccccaacac cttgtgcacc ataaatctcg ccagtctctg
    75121 gatggaagat caacttcagt aaaatatctg ttgcttctgg ataatagctt gcatgatctt
    75181 ttcctgatac atgaatgact tcaaaagaca gttcgttttc tttggctgtt ctttcactta
    75241 aaccggtgga tgcggcagtc attccaaacg ttcgtataat tgctgttcca ataccgcctt
    75301 gatttcttcg agaaattccg gaaatcgtgt ccgcaacttg tctaccttga cgattggctg
    75361 gtgaagcaag agaaataagt gcatcttttc cagtgatttg ctgttttaca acgatagcat
    75421 cccccaccgc ataaatatca ggaatattcg tttgataacg ttcgtctact aggatacccc
    75481 cgcgtatgcc taattcgatt ccagcggtca ctgctagctg gttatcagga cgaacaccaa
    75541 tggataaaat aactaaatca gcagggatgg tacgcccatc atccagtcgc aatcgtttcc
    75601 cgtggtcttc aaatccagcg acagcatgag aagtaatcac ttctactcct tgatgagaca
    75661 attctttttc tataaaagct gccatttctt catccagtgt tggaagaata tgtggtgctt
    75721 gttcgatcac catgacttct aaacctcttc tttttaggtt ttctgccatt tccaatccga
    75781 tgaatcctgc accaacgatc acggcacgct ttggctgttt ttctaatgca tgcatcactt
    75841 tatcgatatc tggtacattt ctaatagaaa aaacattgtc ggcttctgtt atccctggaa
    75901 tcgatggaac aactggtttt gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt
    75961 gtttttgacc ctcatgtacg acttctaccg ttttgttttc gggaaagatg gctgtcactt
    76021 catgatttgg acgcacatct aaacaaaaac gagcggataa tgattctggc gtttggacaa
    76081 gaagattttc ccgttcactg atttctcccg aaagataata aggcaagccg caatttgcaa
    76141 aagaaacata cggtcctttt tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa
    76201 gccgtgtagc agcagacatt ccacctgcta cacctccgac aataacgatt ttcatcttat
    76261 ttccctccta ctgttcgatc aaaccattga ttcatgccac cacgtacatt cactacatca
    76321 taaccagatt tctttaattc tttagctgct tgtttactcc tcatccctga ttgacaaatc
    76381 acatagacgg tcttttcttt gtctccatca tatctgttta tactttgtaa tggaacattt
    76441 ttagctcctt ttatatgccc gccccggtat tctgcaggtg ttctaacatc aagtaacgct
    76501 ggattttcca aaagcttttc ctttaattct gggactgtta tcgattttat tgattgaaat
    76561 agcaaattca ttttctgcac ctcatttaca tttttataaa tacctcatgg ggtatattaa
    76621 acaggagctt tattaatatg tcaaccaaaa tgactcttca tttttttcac tactttttat
    76681 atttgcataa aataccgata aggggtatta tctaattata aaaatagagg agaatgtgtt
    76741 atggagattg ataataaaaa tatcctgaac cgtttgaaac gtacagaggg acaaattcgt
    76801 ggtatccaaa aaatgatcga agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc
    76861 gctgttcgtt ctagtattga tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat
    76921 tgctttgaga aacctgatag taatccagaa gaacaagcgg ctaaactaga acaagcgatc
    76981 aagatgatta ttaaaaagta aaggaatgat ttcaatgaaa gtcgttttcc atattgatga
    77041 attagaaaaa tggtcagaaa cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga
    77101 aacgacgatt gtcgtatctg taaatggcat cgcgattacc ggttatctag actctgccaa
    77161 tgcagaattc ttagatttgc aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc
    77221 taatcatatt tcagaaagtt ctttacctga acaagtaatc gttgtaccag caggtgtcct
    77281 tgatttagtt gaactgcaat cccaaggtta tgcttatatc aagccttagt cagtagaggt
    77341 tgcgacagaa gtgatcagct tcaagaaatt tcggcttatt tttcgaagaa gctacttctg
    77401 gaaaaccgtg aatcaaaggt tgtgaaaacg ctgtcctgca tcaagtaata agaacagcca
    77461 aacaaaaata gcttctcata tttttcgttt ggttgggctt attaccgcag atgcaagctt
    77521 ttgaacaccg tttaaggaag aggttgtgaa atcagcggtt tgacctaagt attagagaaa
    77581 aaaacgggaa aatagctcct catattttgc cgttttttga gctaataccg aaggtcagct
    77641 gattgaacac cgtttaagga agagattgtg aaaaagtcgc ttagtttcga gtattaagaa
    77701 aaattttgtg aaaatggatt tctacatttt tgcataaatt tggcttaata ccgagaaact
    77761 ggcttttgaa taccgtttaa ggaagaggtt gtaaaatcag cggtttgacc tgagtattag
    77821 agaaacaaac ggaaaaatag ctgctcatat tttgccgttt tttgagctaa taccgaaggt
    77881 cagctgattg aacaccgttt accaagaggt tgtgagccta ccatttaact ccgaatcaca
    77941 agaaataatt tcaaaaacaa cttctcctgt ttttggttcg gttggcttat tatcgagaag
    78001 tagctactgg gagtctattt atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt
    78061 attttctttg aaacatcttt cggtactcga cagcatacaa gacaaaagat tgtaaaagac
    78121 cgtaagtgat gacggcaaaa atgactctca cccacagcgt tggtaattct gggaaaagca
    78181 gcaaaaggat catgccaaca tagatgacag cagtcgctac tttgccatac cacttcgctc
    78241 cacttaattt gatattttta gatagcccga ttaatccagc tacaaacata aagccttctt
    78301 ttatcaagaa tagaccaaac aacagccaaa gccacggtcg ataccatgcc atagacaaaa
    78361 tcagaaaaag ttgtgtcaat ttatctgcaa acggatcaag caccttccct aagtctgtta
    78421 cctgattata tctccgagca atgaccccgt ctagataatc agacaaacct gagacaacaa
    78481 tgaccgctcc tgctatataa aatgactgtc tcagcacaaa ataaagataa atcggcagca
    78541 aaaaaatacg gaatatcgat aaaagattcg gaatcgtttg tatttcttta cgccagtctg
    78601 tgcttaattt cattttatcg ccctacctcc tcagcactct tttcttttta ttttagctta
    78661 cacggggaat aaaaccaatg atttcactgt ttttattttt ctttacagac actgatatta
    78721 tgagctaatt tgagataatc acttgctgtt ttcatcgttt actcttcttt cttcctgtcg
    78781 tttgcgcatt tcttttacta gtactttatg agcttttttt gatcctcgct ctaggtcacg
    78841 ctgccgttta cgtgttttat aatcgataaa cagactgtct aaatcgtaac ctgtcgggta
    78901 cagatcttct gctgaacgca ccagacgaac acgctgacga ggcacgtgga ttattttttt
    78961 gtctaaatat acatcaatcg tatcttcacc tatatcttca taaactaacg cttctttttg
    79021 gtattcattg acaaatatcc gatcaccttt tgcaaagagc tgacgttctt ttttattctc
    79081 cttcctattt gtaactgctg tcttaggaag aaattctttt ttctgtgtcc gataggcttt
    79141 ttccgccaag taacgctctg cttgatggat caatttcatc gacatatcca ttttatgagc
    79201 aatccacaaa gcttggcttt ctcctgtttc ttccaacccg caactgatat ttaggacgaa
    79261 gtgcttcccg atcgaaagcc atagcagccg gcacaaaatc ttcatgatct ccggcgaatt
    79321 tcttgatttc tccataatgg gtcgtggcaa tgatcaatgc cccttgctca tacatagatt
    79381 ccatgattgc gatcgctagt gcagcccctt catttggctc tgttccactg ccaatctcat
    79441 ctaaaagtac caacgtattc ctttttatct ttttcaaaat agcagcgatg ttttgcatat
    79501 gaccagaaaa tgtactcaac gcattttcta aattttggtg gtcaccgaca tcgacgaaaa
    79561 tctcatcaaa gacagcaagc tctgtccctt cttttgccgg aacttgcata ccgaacatcg
    79621 ccatcaaagt caataaaccg actgtcttca acacgactgt tttgcctccg gcatttgctc
    79681 cggtgataat caagcctcga tagtctttac cgatttcaac atccaatggt accgcatgct
    79741 caggtaaaaa tggatgtctg ccttgcttga taatgatatg ttccgattta ttgattctag
    79801 gtgtgcttcc attgatttct cggctaaatt ttgcccgagc aaagatgata tctaacaccg
    79861 tgatcgtttc gatcaataaa tcgatcactt cttcgttttc tgcgatagca ccagtcaatg
    79921 ccgccaggac ttgatattct tcactgattt cttcagcttt taacaactga tagtgttcat
    79981 tcaatttaga aatcacagtt ggttcaataa aaaccgtcgt tcttttattt gattcatcga
    80041 taatcgttcc atctatttta tttttatagc tggctttgat aggaatcgta tagtattcgc
    80101 ctttttgaac aatcattgct tcttggatca tttctttgtt tttcggatga cgcaagaatt
    80161 tcagtaattt tgactggatt tctttttcga ttagttgtaa ttgtttgcgt actttacgca
    80221 aattacgtga cgcgtcgtca ctgactttat tattttttat tttctggtca atcaactctt
    80281 ctacatttat caaatcagga agatgtttgc tataagcaaa caacaagggt gtctggtatt
    80341 gattcttatc aaaaaacttt gtgatcatcc gactgcttct aagaaaatcc gcatattcga
    80401 tcaaatcggt tggctgcaga atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc
    80461 tcggcaaccc cataaatgga acatgctgac tgctttctaa gatcaagcgt gcttctcttg
    80521 tttcttcttg acgatccaag acagtttgta ggtttgtcga gaccgtcata tcgctgattc
    80581 gttttttgct atagtttcca attgctcttg cttggatttc ctttttgatc cgatcaaatt
    80641 gtaattgact aatcatttcg ttgtccatat tctctccttt tcttgaagag atacctgtcc
    80701 caaaataaat aagggcctag agataaataa aaggatagaa cacagcttaa aaaagtcatg
    80761 tcctatcctt attagaatac aaagatattg ttttcctgac aggtaccgtt tcataagcac
    80821 accaaataaa ctttgtttat tggctattcg acttactgat tagataccag ctacactcac
    80881 aaaacatacc ccattcttct aggtcttatt tattttccac ttaactatag caagacacat
    80941 tttggttgtc aaactttagt atgttttata gatttacacc attaatgaat agacacctac
    81001 cgcgataatt gctccgatca ttggacctgc gattggtacc catgaatatg cccaatctga
    81061 agttccttta ttagctattg gcaataccgc atgtgctaat cgaggaccaa agtcccgagc
    81121 agggttgatt gcatatcctg ttggccctcc taatgaaaga ccgatggcta agatcaatcc
    81181 acctacagcg aatacgttcg ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc
    81241 aagaactaat acaaatgtac caattaattc tgtgatcaca tttgctggat aattccgtat
    81301 agcaggacct gttgcaaacg ttcctaaaat cgcgctttca tctttcgttg cattccaatg
    81361 tggcaaataa gaaagccaaa caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat
    81421 gatgaaagga acgacaagat tccaactaat tgcccctgtc atcgccatag ctagagagac
    81481 tgcaggattc aaatgagctg ggcccatgaa tccagaaata tagacagcca ttgttacagc
    81541 gagtccccaa ccaaatgcga tgacaatcca gccagaagcc tgtgctttac ttttgtttaa
    81601 attcacagcc gcacacacac catctccaag caaaactagg atcagtgtcc caataaactc
    81661 ccccatgatt tgagtcatat cactgttcat ttatttctcc ccctttagtt cccgcagatc
    81721 tgattctgca atcaatgctt ctaaacgttt agtctgttgc gccttctcct cttctgtcca
    81781 ttcaaaatat ccagcaattg catcgatcac aggttgcttg atctcatcaa gctgatctcg
    81841 ttcaaacaac agatgatttg tacgacgaat gagataatcg cctggggcca aaaccatttc
    81901 ttcttctaaa ccataacgta accgagctga ctcagcgaga ctcaaaccag gataaggtgc
    81961 catttctttt gctaattcaa agatacgtcg ggcattagtc ccgtaaaaat cagcgatata
    82021 agtagcatct tcttcctcta aaccggctgc tactccttct tttgctaatt ctgtcactgt
    82081 ttcttctaat ttagttggat cgaagtttcc acctgaaatc tgatattttt tagagtcgat
    82141 ttctttcgtc tctattccgt attcttcttt taataattgg cgtatcaatc gcaaagctcc
    82201 ttctgccatc ttacggtaat cggtgatttt cccaccagat aaagtcagca aaccatctgg
    82261 ttctctttct aaagaactac ctctggaaat cgtagaaggt gctttttcat cacggctgtt
    82321 ttccaaatgg ttcaacacgt cttctacttc agcacgagaa actttgtttt ccttatattc
    82381 acttactgta tcgacaactt tattgaagct cttgtctgaa atcgatccat tatctccacc
    82441 attgtaatca gaaccagaat taccaatcaa cagtggacga agtcctgccc agcttgcttc
    82501 gatatcattc aatgtgatat ttgcttctgg atagcgatga ttgatcacat ccaatagata
    82561 atctacatct tcttgtgtga ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt
    82621 cgtaccaaag taagtcttgt tttctcttgg aatagcaaaa accatccgcc catcttgctt
    82681 tcctgtatcg aagtatgtgg gttgcggtac aggcagtttt ttcgcatcta caactaaatg
    82741 gatcccttta gttggacgca ttttaggaga gactggacgc gtaaaattca agtttcgtac
    82801 tttatctacc caaggaccac tcgtattgat cactaatttt gctttgattt cgatcacttc
    82861 atctgtcagc aggtcacgag ctttgacgcc aacgatttga tcgccatcat ataaaaagcc
    82921 aaccgctttc attttactta ctagataagc cccatcttcg gcagcctttt tgatattatc
    82981 aatcactaaa cgggcatcat tgttgcggaa atctagatag acacccgcgc cttttagccc
    83041 ttcttttttc aaaaacggtt ctctttccaa tacttcttct ggtgtcagcg tataattctc
    83101 atatttggtt cctgtcacat ttgccagttt gtcataaagg tccattgcta ctttgaccga
    83161 gaacatattg aaggttgttg ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg
    83221 ttttgggatg tgtggggcaa tactttgtac gactgcacgt tcaccaactg tgtcagctac
    83281 tacttccaca tcaaatgtct tcagataacg aataccgcca tgaacaagtt tggtcgagcg
    83341 agaggaagtc ccttctgcaa aatcctgcat ttcgatcaat cctgttttga ttcctgatgc
    83401 tgctgactgg actgccacac cggcaccagt gattccgcca ccgatgatca acagatccaa
    83461 ttcttcttgc tgcatttttt gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat
    83521 ttcctcctta ttcaaatgct tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt
    83581 tttctcggcg ctcttcctcc atttgaactt caaatagctt gccagcttca taaaattcgc
    83641 ggatctcgtc tgtatctttc caataaccga cagctaatcc tgctaaaaat gctgccccta
    83701 gcgccgttgt ttccaagttt tctgctcgtt ggatcggtac attcaagata tctgtttgga
    83761 attgcatcag atattcattg tttgctgcac cgccatctac tttcaataca ggaatcttga
    83821 ttccagtgtc atcttgcatc gtatcgatga tatctctcac ttgataagca atcgactgta
    83881 aagtagcttt aatgatatct tcttttgtcg taccacgtgt caagccaaac atagcccctc
    83941 gtgctgcctg atcccaataa ggagcaccta gcccaacgaa tgcaggaaca acataaacct
    84001 catcttcgct tgtagagcgt ttcgctgcgt cttctgaatc gctagctttt tgcaacattt
    84061 gaagtccatc tctcagccat tgaatcgaag aaccagccac aaaaatactt ccttctaaag
    84121 catagtaaac ctttccattt atgccgtaac caattgtcgt caataaatta tttttggata
    84181 attgcggttt ttcaccggta ttcatgacaa taaatgaacc tgtaccataa gtattcttga
    84241 ccatgcctgg ttcgaatgcc atttgtccga ataatgcggc ttgctggtct cctgccatcc
    84301 ctgaaattgg tgcttcgctt ccataaaaat gataaccttg cgtataacca taaacttctg
    84361 agttagaagt gacttttggt aacatcactc gaggaatatt tagtaaatct aaaatctcct
    84421 gatcccaatc tagctgatgg atattgaaca acatcgtccg actggcattc gagtaatcag
    84481 taacatgtgc ttgtccatcg gtcagtttcc atactaacca agaatcgatc gttccaaaca
    84541 tcaattcgcc tttttcagct cgttcttgcg cgccttctac atgatcaaga atccagcgta
    84601 tcttcgttgc tgagaaatag gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt
    84661 gatagccttc attttttaag ttatctgcaa ttccagctga ctgtctggat tgccaaacaa
    84721 ctgcatgata gattggacgt cctgtatctt tttcccatat tactgttgtt tcacgctggt
    84781 tcgttatgcc aatcccagca atctgatttg gtttgatccc tgattcgatg aacgctccgg
    84841 caataactga ctgtacagaa ttccagattt catttgcatc atgttccacc caactttctt
    84901 gcgggaaata ttgcgtgaac tctttttgag aactgccgat gtgtcttcct ttcttgtcaa
    84961 agataattgc tcgtgagcta gttgtacctt ggtcaatcgc catgatatac gttgattctg
    85021 tcattgtgat tccctccatt atatgcaaac gctttcttta actactctta tcatactact
    85081 gttttaaaaa aagaaactgt caaaatttta ttcttttttt ctttttttga cagtttttta
    85141 ttatgaagct attttttaat caattctttt cttttttcag cgtctctatt cttcttttca
    85201 atcgaataat gtcgtaaggt gacgcaaatt ctgacagata atattcacac tgttttttgt
    85261 ttctctggca aaatgtgatc accaaatcat attctttacc ttcttcataa cttccaatct
    85321 caataccgcc gattcccctc agttctctga tcagatattg ttgaaaagca atacgataaa
    85381 tcggcagact ttctaaatcg attccaatca ctactggttt tgcaatataa aaatcgatca
    85441 tcaaaagaac attggcatac tggatcatca ggtaactaag ctcactatct tcagctcttt
    85501 tcctcggcaa ttgctgcacc gctgtttcct gtaatttcgc cagtaactgt gttaaggaat
    85561 gcccgagcat tttctgctgc tgttccaaaa gccgatcacg ctcatagact tcgatctttc
    85621 caacaaagaa aaaccattca ttgttgatct gggaaatttg gtagcctact gccttttctt
    85681 ctttgatttt caaacgacga ggacgataat gccataacat cgtttcccga atgtaagtat
    85741 ctaaaacagc ggttggtaac tttttgctcc gtgtcaaatc atactgataa aatatttctt
    85801 cgtctactat cgaaaaagaa acaaaaaagc tgtaaaacat cattgtttcg tatacattca
    85861 gttctgctgc tgttcgacta agatgcatca cgatgattgg atcgattgct tgataaagcc
    85921 ggtccgactg atagagtaat ttcttttctt tcaaagttgc gtacgtactt ttttcattca
    85981 atagtctttt tttcgtaatc ccgagccagc aagcaatctg tgcttctgct gatgccgtaa
    86041 acgttgtttc taatacacgg tccaatccta gaatgaacgg tcttttttct ggtgtatttt
    86101 gcaaaaaaag cggtcgctga tcttccggga tatattggaa tagttcataa taaaaatatc
    86161 gtatctgtag ctcttctccg tataattgac cgtttttgat ttgtaatcca aattcctcta
    86221 agagctggtt caattctttg atctttcgaa aaacagaaga ctcactgata ttaaacgctg
    86281 tcgctaatcg aacgatggat acttgctgat gttctaataa atagacgagc atcttaaact
    86341 tcaagctttc ctgcaataaa aacaatagaa ttttttccaa acttacatct tcgtccaaca
    86401 caagcagcgc ctgatactca tttcgctgta cttctacttt tttttccatc attcggccaa
    86461 tcaattcaat atcttccaaa tattgatcaa acgaggcttt tgacagttgg acatgctctc
    86521 tcaaaccatt gatagaggct gttccccctt gcagaacaag gtttcgcaaa atcaggatct
    86581 gtaactgctc ttttctttct aataatgctt caatcttcat aggcagtatt cgccaccttt
    86641 gaccacacca tatctggcac acggtattct ggctggaaaa atagctcatg ttcttctaac
    86701 tgttcaaacg tccatccttc ttctactgca attgtcagtt ggtcaagaag gtggaacatc
    86761 tcttttttgc tttggagctg tgctccaaga atcttttttt cagaatcaaa tagaaatttg
    86821 agtttaactt tgctttgatt tattgctgta agcttgtagt ctacgtatcc tgtttcaatt
    86881 ttttggggat aaaaaatgct ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca
    86941 aaccactgat tgccgactgg acgataggtt ccctgatcct ttgtctgtga gccactaatt
    87001 gtcttgctga catttcgtgc tgtacggaca gcgttattta ctagagaaac atacattttt
    87061 tcatttgttg gacgaaaagt tacttggatc gcatcgccaa tcgcataaac gtctttttga
    87121 gacgtttgca agtagtcatc tacccagatc gttccatcat cattcaatgt taattgttct
    87181 tcccacatct gattgtcggg acgagaatga ttagccagca aaacaagatc atagttctcc
    87241 tttctttttt ccgtctctac aatagcggtt tcattttcgg tcaaagacac aacttgttct
    87301 tctaaaaaaa gtgaaagccc ttgatttttc atttccttta ctaacggttc tgtcatttca
    87361 ggatctagat aacgaaaaag aattgacttt cggctttcgt aaagatggat ttgttttttt
    87421 cttgatgcca acccttcagc tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt
    87481 ttctttgcat tcatgatctt ttgctgaaga gccgttacgt tttgtatctt tttaactggg
    87541 tgaataagcg ttgattctgc agaaatattt cgaaactttt gatttgaacc agtcgctaga
    87601 atcaaacgat caaaatccaa tttttttcct cctgctaaaa cgactttttt ttctttcaat
    87661 cctattactg tcttttctgt gtaaacatgg attcgatagc ggtcagtcag ttcttctttc
    87721 gtgatccaag aaagttgttt ttcactggta acctttcctt ttaatatcaa attgaacccg
    87781 ctgggaatcc atcctaactt cgattgcttt tcaatcaaat atatttcact tgttggcatc
    87841 aattggcgaa gataaatagc cgcatgaatc cctgcaaacg acccgccgac aattacaatg
    87901 ttcatctgca ttcctctttc ttataaggtg ttctactgct aacttctcaa tcagaaaaca
    87961 cagtattcat aactcgcttt caaccttatg atacatagcg atgcagcaga atcaaccctc
    88021 tccatcatac cgtgttctag gtctgcttct caataatgta ctagtggacc taattgaaga
    88081 gtatcatttc tgaagagatg tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct
    88141 tttacgtacc tctgtccaaa taggaaacag aaaagaacga aaagacgagt tttagcgtgt
    88201 gtaatgtaat agttagaata gacacatcat ttatttttaa ttctttaatg cttcaacaac
    88261 tgcttcgcct aattgatgag cagattgagg attttgccct gtaagaagcc gcccatcaat
    88321 ttgaaccact tctgaaaaag ctgctccaga ctgatagttt gctccttttt ctttcaaagc
    88381 atcttctgct aagaatggta cttcattcgt tgtaccattt gcttcctctt cttcattact
    88441 gaacccagtc actgtacgtc cagctaaaat agattttcca gactcatctg taactggcaa
    88501 caatcctaca acaccgtgac aaacagctga gaccagccct tcatttcgat agatttgtga
    88561 agcgactttc ccaagatcta ttgcttctgg aaaatcccac attgttccgt ggccaccagc
    88621 ataataaacc gctgcataat catttgcatc cacatcatca ggacgtaagg tatttcccaa
    88681 ggcccgattt ctgaaatctt catcttcata ataggcccaa tcgatttcat ccatattcaa
    88741 aacactttgc ggttctaaag gtacatatcc gcctttagga ctgacaaaat ctgcatcgat
    88801 cccatttttt actaatacat cataaaaatg agtcagttca cttaaccaca aacctgtagg
    88861 acgctcgatc ttttcgtact tttctacatt tgttaatacg atcaatactt tcattttccc
    88921 atcctctttc tataaaaatt gcgttattga taacgtgctt tcaagaaatt atataatggg
    88981 cttggcaaat aaagctcttt atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca
    89041 cggttttcca ataatttctc tgcccagtca ggatcgacga tcatttgctg tccaattgct
    89101 gcaagttctg catatttcaa tacctgctct acatcttcgc gattgcggat cccaccgaca
    89161 ccaactaaag ggatctttcc ttgaatcgtg ttatggacca cttcaagaat cgatttatcg
    89221 ttgtatttat cagaacgagc tattcgatca tacatattta atgaaacatg cagataatct
    89281 agttttgact cacgtaatcg ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt
    89341 gtttcatatt cttctggtga gaaacgatac cctacgatga atggtttttt agcatattgt
    89401 tcaaccgctg agaaaaccgt tgttaataat tcttcaataa aacgataacg attttccaag
    89461 ctaccgcccc attgatcctc tcgtctattc gaatgcggag agaaaaattg ttggatcaaa
    89521 tacgtatttg ctccatgcaa ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg
    89581 gctttgccaa atgcttggat tgtttcaacg atttcttctt cactcattgc tcgtggtgtt
    89641 tccgcatcag gacgttttgc tgcaactgca cttgcagata caggctgttc tccagataaa
    89701 gttgctcgac ttgtcattcg tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct
    89761 ccttggattg ctttcgctag ctcacttaat cgaggaatca tttcatcatg agcgatgctg
    89821 agttcacctg gccagccttt tccgatatct tggacatttg cagcaccagt aataacagca
    89881 ccaagacctt tagaacgttg tttataataa gtgatctcat cttcggtgat cactccattg
    89941 aaaaagctga gttgtgtcgt cataggcgac agcatcaatc ggttcttcaa ttgaactcct
    90001 gaaggtaaag taattgattg atcaaattgt gacattgtca ttcctcccat aattcgctct
    90061 gattaaccta tcgagaatca tctatcatta aaccactcga ttagttgtac atgcaactaa
    90121 tttgcttaaa aaattttatt ttttttgtaa cttatctgtt gcatcattca ttgtctgggc
    90181 taattcttta cttgcttcat ttcccaaaac tgctttgtat gactgatgaa gttcttccca
    90241 gctaccatcg acttccttag caaattggcg tccttgttct gtaagataaa ctaatgacaa
    90301 gcgaccttcg ctaactgttt tgacgtatcc tttatattct aatttttcaa caaaacgagc
    90361 aatcgttgat ggggcggaat agagcatttg tgcaatatct ttttgtggta ttccatcttt
    90421 ttcttttaat atcaataaaa taaaaccata agttggtgcc agacctgtcc ttctaaacgc
    90481 ttcatcagct aatttattca acatgcgatc tagctttttc acgctaaaga aaagacattc
    90541 atctaacgct ttttcaattg gcttttccat ttcatcccct ccattggcgt acatatcata
    90601 gtatgatttt tattttttgt caaaaaacac ggaataatgt ccgcctcctc tgtctagatt
    90661 tgaaaaaaag aagtagccat cttcaaagaa tttcggcttg ttttcgacaa aactccttct
    90721 ggaacaccgt gaatcagagg ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt
    90781 ttgtgaaaat ggctttccac atttttgcat aaatttggct taataccgaa aaactggctt
    90841 ttgaacaccg tttatatcag aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca
    90901 gccaaacaaa aatagttcct catattttgt cgttttttga gctaatgccg atggtcagct
    90961 gattgtacac cgtttattga gaggtcgtga gcctgccgtt gaactccgag tcacaaggag
    91021 caatttcaaa aaacagcttc tcatgtttta tgactatggt gacttgtgat cgaggagttg
    91081 gcaggagaat actgtttatt cgtaaagaag gggctgtgac aagacttttg tcacagtccc
    91141 ttatttcctt ttattatagg atttcgccat ttgattggat gacttgtctg taccaatcaa
    91201 atgatttttt ctttaaccgt ttctttgtgc cattcccttc gtcatcttga tcaacataga
    91261 tatatccata acgttttgac atttgtgaag tagaagcgct gacgatatca atgcagcccc
    91321 acgacgtata accaaataaa tctactccat ctttgatagc ttccttcatc tgctcaatat
    91381 gctgcttcaa atagtcaatg cggtaatcgt cttgaacggt tccatcttct tctactttgt
    91441 ctatagctcc gagtccgttt tcagctatga acagtggttt ttgataccta tcatataatt
    91501 gattcaaagc aattctcaaa cccttaggat caatctgcca gccccattca cttgatggaa
    91561 gatacggatt tttaattcct ccagttatta agtttccttg gaccccttcc caattttctg
    91621 gatgagcact gactgcacta gacatgtagt agctaaaaga aacaaaatca acagggtagg
    91681 ctgctaagat ttcttcatca cctacttcta tttttaagtt gacacctttt tcttcaaaca
    91741 ttcgttgcgt atacgctgga taatatcctc ttgcttgaac atcactaaag aacaaagaag
    91801 ttctttgctg ctcaattgtt ttttgaatat catccggatc acaagaatac ggaacaagaa
    91861 gctgatagct gatcatacaa ccgacttgtg aacctttgat aatttcatga caagcttttg
    91921 tagctaaagc acttgctata aactgatggt gtgcaccttg gtatttcgct tgttccaaat
    91981 ggggatggtt ttcttcaatt actcctacgc taacaaaagg gtgatgtttt acacagttga
    92041 tctcattgaa agtcatccaa tattttactt tatttttata gcgtttgaat actgtttcag
    92101 cgaaacgaac gtaaaactga ataagctttc gattaggcca tccaccatat tctgtaacca
    92161 aatataacgg catttcataa tgggaaagag aaacgactgg ctcaatccca tattttagac
    92221 attcgtcaaa cacttgatca taaaaaaata atcccgcttc attgggtgtt tcttcatcac
    92281 catgaggaaa aattcttgtc catgcgatag acatccgata gcatttaaaa cccatttcag
    92341 caaataaacg gatgtcttct ttaaagtgat gatagaaatc atttccgtgt cttttaggat
    92401 aataatactt gctgttggga tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc
    92461 attgatctgt ccatttttct ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag
    92521 ataatccttt ttcatctaca tcccatgcac cttcacattg attggccgct gtcgctccgc
    92581 cccataaaaa tccttctgga aattttttca tatcgatccc cctaaacttg ctagttttct
    92641 tgtttgatta tttttcttat gactagtaat gatatttttg aatctactac tatttgtaac
    92701 aataaccatt gtggtcggat catatccctc tttgattaat tcttgcaaat ctacttccat
    92761 caataaatcg cctttacgta ctttttttcc atcagatact gcactttgaa agtatttgcc
    92821 atttaaattc actgtatcaa taccgatatg gatcagtatc tctgctcctt cttcactgac
    92881 taccccaatt gcatgtttcg ttggaaaaac tgttgaaact tccccgttta cagaactgta
    92941 gatttttccg tccgttggaa tgatcgcaaa cccttctcct aaagatccac tagcaaatgt
    93001 tggatcgttt acttcagata aatcaattat ttcacctgct gcaggagtac ttaacactat
    93061 atcctttgag gattcttctt cgatttcatc cttgtagccg aaaagaaaag ttagcataca
    93121 ggaactgatc atcgctaaga aataactagc aactagccaa acaaatcctt caccaatata
    93181 aatcgggaaa gacaaaatac ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc
    93241 agctactcca ccaaatatag ctccacatat acaagcaatg taaaatggtc gtttatattt
    93301 aagagtcaca ccataaacag caggttcagt gattccaaat aatgctgtga taaatgcaga
    93361 taaagataaa ctttttactt tcttattttt cgttttcaaa aataccccaa gtactgctcc
    93421 tgcttgcgca gctacggcag tcatacatgc tggacctagc gtatcacgac cgtagaaaga
    93481 aagattgttg gtcattactg gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg
    93541 ccaaaatccg ccgataacag caccagataa aacagggcta aagttataaa cagctaaata
    93601 tccagctgca atcagctgac ttgcataatc tgcaattgga cctacaacta ataaaataat
    93661 tgggaccatc acgatgatac aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa
    93721 gcttttgcga atcttaggct caatataaga aagaacccat acagctaaaa ttgctggaat
    93781 aaccgtactt gtataccttg ctagaatcac gggcatccct agaaatcgca aggttatgct
    93841 atcactatat gcactgacaa tcgtaggata aacgagagct gctgcaataa ccatagcgat
    93901 gaaacgatct gtattgaact tttttgcagc agtatatgcc aaaaatatag gcaagaaata
    93961 gaaaaatgca tcagctgctg cattcaaaat gatataagtt cctgattcag tagttaataa
    94021 attcaaactc gtacataagg cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc
    94081 taaagtcggg gcaaaaacag cggcgatcat gtctataaaa gagtttaaaa tgcctttttt
    94141 ctcagttcct ctctttccac tagatgcatc atcttctagt ttcatttgtt tggataatac
    94201 gtcatacact tcatttactg catttcctac tacgacttga aacattcctc cagcttcgac
    94261 tacagtgatc acaccttcta gttgctctac cttctcctta tctgccttct tattatcttt
    94321 tagggtaaag cgtaaccttg ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc
    94381 gacaaatcgg acaatatctt tcgctaattg tgaatagtcc atttttactt cccccttgtt
    94441 aattaatgcc gtagcaatcc ttcttacaag ataagtatag ttcttagaaa acggtttctc
    94501 cattcaatta gtaccccttc taatagcgaa cgaaactgta caatagtgtg tatggtttac
    94561 ttagccgagc aaatcactca ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc
    94621 aaaggagaca aaaaaaacac gcttgatctt cctttgctga tgtataagtg gtatctcaag
    94681 ctgatactct gtgatttttg tttctacttg gcttttgata agacagcaca acaaagtctc
    94741 ggcatttaac ggaagcataa ctaggtcaga actaggagag attagttgct gaatggaatc
    94801 tatcttttta tggattttta tttgattttc ttctttgtag taatgatcaa gcagttttgt
    94861 tttctttttt tcttcaacta tggtcagctt ttcacgcaaa atctgataaa tctcccaaga
    94921 ttcaaactca tctagaacac taggtagata aatcaaatca tcccactcaa aaataagctc
    94981 ttgctcagta gtcaaaattg catcatattc cgttattttg tttgattgat ttttgaaaag
    95041 atacactggt tctatccggc attctttgat ccacatgttc atactttgtt gaatctgttt
    95101 ttttagtgta ttcaaaatac taattggctg gttactaaca atcagtatct ccccttgttt
    95161 tcgaaaagga ttcaacgctt cggcaagata caaaacaaaa taagcgcttt cagctaaatt
    95221 gggacgtaat tgaaaatgta ttgggaagaa acgccgaatt aaataaattt ctaaaggata
    95281 acgggagatg atcttttgta caaaatgatt catggtatta tgtccggcat ccatcctcgt
    95341 tttcatttgt ttgatatggg ttagcaaaaa ttctgtttca tggaacaata acgaaacagg
    95401 aagatctaaa aaacgaataa tcgtttgctc aaatgctctt agattcttct ctatttcttt
    95461 gtttttttca tcattaatca tcaaataatt taactctaat agtcgatttt caattgcttg
    95521 tttttcttga ttggaaaaag aatagggtag ttctgatgat aacttttttg tgagggtctc
    95581 taccatcgga ataaataatg tatacctgtt tgttttttct aaaagttctt ctcttcgaag
    95641 aatggtcaaa acgagatacc gagaaaatcg tatgaagtcc tctcctgaaa caatataatt
    95701 atatgatata agaacctctt ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat
    95761 tgtactgatt tgttgaggaa accttgcgtg ttggatcacc gtttctgttc caacaagaga
    95821 taagaaaatt ctttttgaat tttcttcagc aatgattttt agtccttttg tcgacgaaac
    95881 ttccagttct aatttaggat tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga
    95941 tacggctgtt tttgaaagaa agaaaatgtc tgctatcttt tgcatactga catacccttt
    96001 ttgaaacaat aagtacgtca caatagttga agctcggaaa tccatgctgg aatagatttt
    96061 atgtttcgtc acctcttgta tgatttgctg tttagtttca gattcaattg attctaaaaa
    96121 ataacctgaa cgtttatttg actggataat gattttcttc tcgtagatag caagcatatt
    96181 tttgatttca gtttggatcg ttttcttgtt ggtacctaaa gcttcagcta attcttcgct
    96241 tgttatccaa tgattcttat ttcttaatac tacaagaatt tggagttgtc gtttatttaa
    96301 gtccatttgc ttacacctcc tctttgttat aaacatacag gaaaaacctc tcaaatgaca
    96361 atagtcactt gcagaggttt aaaaatcatt tgaagaaaga tctatattca aggtttttac
    96421 cattagactg gataatttgt ttataatatt caaaactatc ttttttcaat ctcctaaagc
    96481 ttccgctccc atcattttgt ctatccacat aaataaagcc atatcgcttc ctcatttccc
    96541 ctgttcctgc cgatacgata tcgattggtc cccaccataa gtaaccaatg atttggcaac
    96601 catcttttat tgcctcagca atttcctgaa gatgtatttt tagatactca caacgttttg
    96661 gatcgtggat tcgattcaat tcatctggtg cttcatctag tccaatgcca ttttcaacta
    96721 tgaataatgg taattcataa cggtctgtca attcattcat gacataacga aaccctagag
    96781 gatcaattgg ccaactccaa ggttcgggag atttttcctt caaaaaggga ttatcgaaac
    96841 catttgctcc gccagtatct acgcgcattt ctgcttcttt tttatagaca gcgctacgat
    96901 aataactaaa tgcaatataa tctgccgtgt tagcttgcaa gcactgttcg tctccatcta
    96961 acattactgg tgcacagtct tcttccttcc atgtcctgta aatataagct ggataatgcc
    97021 ctcggcacat gacatcggag aaaaaccatg aattgcgttt aaattgcagt gaaccaaata
    97081 cattctctgg atcacagtca tatgcataag tcgctaatgc agaaaagcta cacataatac
    97141 ccatcttagc atttggatcg attttcttta ataattgagt agcacgggcg ttcgctagaa
    97201 acatatgatg acaggcttga taaatcactt tttttgttag tgtagcaatg ggttcaagtt
    97261 tatctctagc agggaatgta accaatccag cagctgcata aggcatacga aaagcattat
    97321 tgatctcatt aaaggtcatc caatatttca ctttcccttg gtagcgttca aagacggtct
    97381 gcgcataaaa ttcaaaaaag tcgattaatt ttcgattttg ccacccccca tactctccta
    97441 ccaatgctaa gggggtttca taatgactaa gagtaattac tggctcgata tcatatcgat
    97501 ttaatgtatt gattagtctg tcataaaata cgagaccagc ctcatttggc ttctgttcgt
    97561 ctcctcttgg aaatattcgt gcccatgaga tgcttgttcg aaaagctttt aaacctaact
    97621 ccttcagtaa acgaatatcc tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa
    97681 gataatggag tgattcgtct aaagcaatct cccaaatctt tttttcttca ttccactgaa
    97741 tactaggatg ctggttcatg atcccgataa gcgtatctgt cacattaggt aatttgccgt
    97801 cttccaacca tgcaccttca cattgattag cagctactgc tccgccccat aaaaaatttt
    97861 ctggaaatat ttcctgatcc ataactatct ctcctcgcta tcaacaattt cttacttgaa
    97921 gtatagctcg attcatcaaa acaagatatt ctaactattt cggcagaaga gaagaaaaaa
    97981 attgaatgct actcttcatt tttaatattt atatataaca aatacataag acgatccccg
    98041 aaagaaaaca ctcgttaacc atctaagcaa acgatttatt ttacttccaa cacaaatgct
    98101 tcataaggtc gtaaatcaac tttgccgtct ggtaactgtt tcagaggata atttgacaca
    98161 ataaccctta aagcttcacg tggaatagtg aacgtctgct tttcactgga catattacaa
    98221 ataacgagcc attgctggtc ttcatatatg cgagaatagg caaagacatg ctcaggcgtc
    98281 ttttccacta attcatagtc tccccagacg atgatgtcat gttttctacg caaatcgatc
    98341 aatttcttgt acatataaaa aacagaatct tgatctgcta acgcttcttc tgcattgatt
    98401 tctttataat ttggattgac tcgaagccat ggaattccag tagtaaaccc agcatgttca
    98461 cgggcgttcc attgcatagg cgtcctagca ttgtctcttc ctttcgcctt gattgattcc
    98521 aagatttcct cttttgtaaa tccagaagaa atcctttcgt tatacatatt gattgtctca
    98581 atatcttttg cttccgcgat atcatcgatt gggcaattcg tcatgcctaa ctcttctcct
    98641 tgatagatat aaggggtgcc tttcatcata tggagcaaga tagcaaacat cttggcactt
    98701 tcttttcgat acacatcgtc atttccccaa cgagaaacaa ttcgcggaag atcgtgatta
    98761 ttccaaaata aactattcca tccttcatca cctaaagatg tttgccattt ggataatact
    98821 tttttcagtt cagagatact caagggtttc aaatcccatt tgtctttgcc ttcttgctga
    98881 tctaatccta tgtgttcgaa ttggaagacc atggataatt catttctttt aggattggaa
    98941 taaagtttag cgatttcggt agttgctccc catgtttcgc caacagtcat cagatttttg
    99001 tcaccaaaag ttgcttgatt catttcctga agatactcat gcagtttagg tccattactt
    99061 gtgatttctt tatccggttc tttgccaatc atatcgatca cgtccatacg aaagccccca
    99121 atacctttat cgatccagaa attcatcatt tgataaatct cttggcgaac ctgtttattt
    99181 ttccaattca aatcaggttg acgcttgcta tacaagtgca gaaaatattg ccccgttttt
    99241 tcatccaact gccaagcaga accactgaat attgagcgca atctattagg ctcttttcca
    99301 ttgaccggat cacgccaaat ataatagtcc cgataaggat tttctttatt tttacgggcc
    99361 tcaatgaacc acggatgttc atcagaagta tgattcacca ccagatccat gatgatacgg
    99421 atatttcttt tctttgcttc ttcaatcagt tcttccattt cttccatcgt accgaattct
    99481 gccatgattg cttgataatt actgatatca tacccattgt catcattcgg agactcgtat
    99541 actggagaaa gccaaattgc atcaatacct aaatacgcca gataatccaa cctggaaata
    99601 atcccccgca aatctccaat accatcttga tttgaatctt gaaaacttct tggatagatt
    99661 tgataaacaa ctgaatggtg ccaccatttt tgttccatga ttttcctcct ataaatggat
    99721 gtttctattt tttgcttaat catttatttt agtctctctc actaaaaatt gtaacgcttt
    99781 caaatagcta ttgcaccttg ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa
    99841 ttccctcaac tgttttttga aaggattgat ttatgcaggg aagaatgctg atttcacaac
    99901 ttctttataa acggtgttca ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat
    99961 ataagaaact attttttcgt ttttttctct aatactcagg ccagaacgct gatttcacaa
   100021 cctctttatt tcacattcaa tatccacatg aatcccacaa atacaaagaa cagaccatac
   100081 atgattggag atacttcttt tcctctttta gctgctaaca tggtaattgg atagaagata
   100141 aaacctaatg caatcccatc tgaaatacta taagtcaaag gcatgccgat caagatcaag
   100201 aatgaaggga tcgcaatctc taagtttccc cattcgattt gtcgcaaaga ttgtgccatc
   100261 aatactccta ccataatcaa tgctggtgca gttacttgtg aagtaacaac tgatagtaat
   100321 ggagaaaaga acaagcctaa caagaagaaa atccctgtag taatagctgt gatacctgaa
   100381 cgtcctccaa cggcgatacc agctgatgat tctacaaatg caccaacagg agaagtacca
   100441 aataaggaac ccgcaagcat agctgttgaa tcagaagcta aagctttccc tacacgaggc
   100501 atcttgttgt ctttcatgaa acctgcttgg tttgctaatc caactaaagt tcctgctgta
   100561 tcaaagaaag tcacaagcaa gaatgtcagc actacgaccc acatttgaag agaattgatg
   100621 tcccctacat gttttaatgc aactaaaaat gttggtttca aacttggtgc tgcggcaaca
   100681 atttcagaag gcattggaat caaacctgtg accagaccta aaatcgttgt agctgccatc
   100741 ccgatgaaga tgccacctgg tacacgccga acaagcaaga tagcagttac gactaatcca
   100801 aaaatcgtca accaagtaga accaacgttg agtgatccta atcctactaa tgtagattca
   100861 ttcgaaacga tgatcccacc ttcgctcaga ccaaggaaag cgataaatag accgataccg
   100921 ccagaaatcg catatttcaa atctgctggg atcgcatcaa tgatcatttc ccgcagcttg
   100981 aagattgtga tcaaaataaa aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc
   101041 catggaatcc ccattcctaa acaaacagaa taagcaaaaa aagcattgat cccaagagca
   101101 ggtgccgttc caattggata acgggctaat acccccatta aaacacagcc gatcgcactg
   101161 gctaatgcag tcgcagtaaa cactgctcct tcatccattc cagaagcacc taatacagtt
   101221 ggatttacaa ataagatata agccattgag ataaatgttg taaatcctgc cagtacctca
   101281 cgttttaccg tggtgttcaa tttttctatt tcgaagtagg aaagtatttt ttccttcatc
   101341 ttccatcccc ctaaatccga acattataaa taaagtccgt taaaatttcg atttattaat
   101401 aaaacaacgt gttttatcat atactaaaac aaaacataag taaacaaaaa attcggattt
   101461 taagcaaaat ccgaacgttc catctgatta aatcccttta tctcaaagtt catgaacatt
   101521 cactttggat tacaagaatt tctttattgt gttaataaag tttcttcatt ctagtctcaa
   101581 aaatcaatag tgtgatttga ttttaccatt tcgtttctgt tcttctttga tgaattcttt
   101641 tgtatcgaca ggattcagat tatttagtcc gccaaaacct gtatcgacca tttttcccca
   101701 gccttttgtc ttcttttttc ctgatttgag attctttgct cgaagcttct cactatgttt
   101761 tgctcgccga tacttcaaat aaccttctaa tcttcccatt agatccactg tttttcctcc
   101821 tttttattca atcagacgac tcagcttttc ctataacaaa ataattgcct tcagggtcag
   101881 aaaaattgaa agtcaagatg ccattgttat ctgtgatttc tcctggttct tccatgttct
   101941 cgtagatagt ttcaaattca tccgtaaaaa atactaaaga aggcggtggt cctaatacct
   102001 ctggtgaaaa cttctgcaca aatacttttg ggaatacgcc taattctaat tcttttgaga
   102061 tgctgagtac aatactctta aattcttctg gcaattcgat tgtttcagaa atttctgcac
   102121 caattttttc tacaaagaat cggcaaatca tctctacgtc attgacataa agcatcacac
   102181 gtgttttttg tatcatcatt tgacccttct ttctatttga ttttctttcc tgtctataaa
   102241 ctgattccat tatatcacga aagcgcaaac accttgtgtt tgatactatt caagaaaaat
   102301 aaagcaatag tttctaggat tgctttcctc cacaaacttt ttttacaaaa aaaaaacggc
   102361 aatgaatgcc gctcttctta tttcgattct tttataattc tgcttctgat tcatctttgg
   102421 ttagtgtgtg tctaacacta gttacgccaa atgtttgatc gattttttcc ttcaacgctt
   102481 cgattgcctg tgcgccacgt gccgcttgtt cagcattgat ggattcaatg accagcaccg
   102541 ctgcttgtgt ttcttccgtc agcatcgttc tttgggcttc acgccagttg agacagcgac
   102601 agatcgctcc ttgttcatca tagtagatga tttcttccgg aagtgccgga gcatcggaat
   102661 ctgcacctaa aggcaggaat gattcgccac cttgggcttt tcctaaatgc agatttcctg
   102721 cgatcttttc gatatcttct ccgccgcaag gtacgccaaa agacaaggaa atactattat
   102781 agatatctac aagcggattg attgggaaaa attcccgttc ttgatttgca cgtttcagta
   102841 aagcttcaat agaagaacgt gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc
   102901 actgagcaat gacttcgttt tggctgaatg tttcttctgt aaggtaattt cttgcttctt
   102961 ttgctgcctc attcaataat tgatgatacg tttcttcact ttcttttgtt acgtggttat
   103021 cgataccatt tacggataag atttcgatgg ttgcttcagg aaatacttcc cagaaattgg
   103081 tatccactat tacttttttc atctgttcac acctcttgat tagaataatt gcttgatgaa
   103141 tagatagtct gtttggggat catctcccat ataaaaaaca tgttcgctgt atttgataaa
   103201 tcctttttta cgataaaaag cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat
   103261 ggctgttttt tgcttttctt ttgctttttc aatagctgta tctagtaaaa atgtaccgat
   103321 accttgatgt ttaaattttt ttcgtacata gattcgctcg atttctaaaa aatgatcagc
   103381 catttcctca gattgactcg ttcctgtatt tagtttcagg taacccgcag gctcttgatc
   103441 gatactcact aggaaaaaat cggtttcttt atcttttagc tcttctgtaa gtttttctag
   103501 tgcataagct tgtgtcaagt aatcagctaa atgctccggt gaattatctt ttccaaatgt
   103561 atctgtaaat gtttcgatac taatttgttg tagtaagtct acatctgaag gcgtcatttt
   103621 tcgaatttcg tatttattca cggcttcttc ctttcaatag atccttttat tgcctttttt
   103681 gacaaaattc caatcttcgc taacattctt gcatacttta tttaaaagat attccaattg
   103741 ttttgcttct tcgtcagaaa gaccatttaa tgcgactgca ttggagtagt tgttttctcg
   103801 aatgatgaaa ggatagattt ctgctccttt tttagtcgga tagagtttct tgattttttt
   103861 attttcttta tcttccaaac gttcgatcat cccattggat tctaatttct tgattgctcg
   103921 ggcagtcgtt gtacgatcta ctttgatcat ttctgctagt ttttcttgga tgattccagg
   103981 attttcgcaa atacgtacta aataaagata ctgtcctctt gttaaatcga cttctttgaa
   104041 ttcgatattg ctaatagaat ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc
   104101 agccatttca tcccaccttt attttgtttg taagactagt tcacctcttt ttgttgcaaa
   104161 tgcaactatt ttcaataaaa aaatcaagtt cttttttaat aactagtaaa gtatattctt
   104221 ttcttctttt acgaaaaagc tcaatcacaa tagatctggt ttttgttttc taaaataagt
   104281 aactgtttct tcgttgttcg cttttctgat agctttggca actcttctat actaaaaaat
   104341 cccatgttgg aagtttcatt attctcaata aacttggcct cttcaccgtg gatcgcacaa
   104401 gcaaaaacaa gtttataata ctgaaaaagc tgtggaatat ctttttgttt attcgtatca
   104461 aaaactgctc gcaactgagt agtttctact gttaatcctg tttcttcata gacttcttta
   104521 cgcacatttt cttcaggaga caatcctatt tcagcaaatc caccaggaag tgaccattct
   104581 ttcgtccgtt tatcttcgat tagtaggacc ttgccatctt ttttgatata ggcacgaaca
   104641 tctacttttg gtgttggatg tccttctcca atcgttgtga gcgcttttat ttcttcgaaa
   104701 gtttcttttc ctatttcact gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc
   104761 tctttatcaa atacatcttt tccataatat agaccagctt gagaaagtgc gagtagtctt
   104821 ttgtattgag ccaacttctt gttcattctc ttcccttctt ttttagcaaa tgtatgacta
   104881 tgtaatcaag tatagaagac tggtttaatt acggcaaata aatataatga aatctgcaat
   104941 aaataaaaga atgttaaaaa actcgagaac aaaagacatt cgacttctct ggggctgtga
   105001 ataaatggcg ttcaaaagct tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga
   105061 cgagctattc ttgtctggct gttcttatta attgaggcag gacagctttt tcacaacctc
   105121 cccactaaac ggtgttctcc taccaactcc tcggtcacaa gtcacaatat tcatcaaaca
   105181 tgagaagctg tttttttgaa attgctcctt gtgactcgga gctgaacgac aggctcacga
   105241 cctctcggta aacagcgttc aaaagccaat ttttcgatat taagccattt ctttgttact
   105301 tattgtcatt gatttctttt tctaatttat ccaatagtgg agtgtaatcc acttgagtat
   105361 agtctgttac atcaaattca tatagtgttc ctaactggaa tttgtcatat tttcggtcgg
   105421 cgatgatctt tttgaatgct tctttcgtaa tatgattggt ccctttcgtt cgatcttcca
   105481 atacgtcacc atagtgataa tgatccatga tatagctcaa atgacgatct gattcacgat
   105541 cacgtttgta ccgacgttgc cataacactt caaagtctgc tgttaggcga atcgtgatca
   105601 cctgatatcc gtatctatct gctaattctt ggagtcgtgc tttttgctta tcactaaaag
   105661 gatactcgga cacaatcact ttctttcctg cttccatata caaatcaagt gctttgtaat
   105721 agaaaggata aactttcgtc ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga
   105781 tggattctgc atagtattcc ttgatttcat cgagcgtgat ggcatacata tctggaaatc
   105841 gttcccgtag tttattcata agatatgttt ttcctgtacc aggactacca gccagaagaa
   105901 tgaaatactt tttcatctca tttatttcct aagttaattg gactttccaa gttcataaac
   105961 agtccaatct tatgcttgac aatttctttt actgcttcat ttgctggaat cacattatgg
   106021 cgaagagact tgttgatttc tgtttcacta aaggtttctt tagctacttt cgtccagttg
   106081 actaaaagct ccgttccaac attgaatttg cggatcccgt tattgatcaa ttcaggataa
   106141 tcttcttctt tcacacctgt tcctccatgg ataactaggg ggacatcaac tactgcatcg
   106201 atttctttca gcaagggaat gtttacttct gtttttgatt tgaattgtcc atgattcgta
   106261 ccgatagcaa tagctaatgc atccacgcct gtagcttcta caaatttcac tgcgtcttcc
   106321 ggtcttgtat ataccttgtc attttcagcg acatggattc cttcttctgt accgccgatt
   106381 gttccaagtt ctccttctac ggaaacaccg tgcgcatggg catattctac tactgctttt
   106441 gtcttcagga tattttcttt gaaaggcaga tgggacccat catacatgac agaagtatat
   106501 ccgctgtcga ttgcttcttt gatatcatca aagtctcggg cgtggtctaa gtgcaagact
   106561 gcatcaacca tttcttcttc tgcaatagct gtcattacat tgactaacac atgcatgccg
   106621 atatatttag cggtatcgac acttgtttga ataatgatcg gcgaacccat ttcttttgct
   106681 gcgcctagca tagcaggaag catttctagg ttatgcgtat taaaagctcc aactgtaaaa
   106741 ttcaactctt ctgctgtttt tgttacttct ttcaatgttg tatacatctt ttattgccac
   106801 cttatctttt tatttgtttc ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc
   106861 cattttgccc atgtagtaat cgatcccttg atcatcacca gcacgagcag catctgcacg
   106921 cttctggtta tagaggctca tcagtttttt atacccatct ccgatcgtct gtttattttc
   106981 caaactcttt tctaaatatt ggatggcttg atcgttcaca tacaattcag aaaatttaag
   107041 tccaacttgt tcataaagtt tcgctaattt atgcttatct tgggtagtgg aaatctcttg
   107101 tttcaattgt gtaatctctg attgaatctt ttcggtcgct tctggcgata gggctttttc
   107161 ttcttttgcc agaacttttt tcggttcttt ttcttttttc ttaaaaaatc caaacatcat
   107221 tttccctact ttcaattaaa atgctttcaa aactgggctt aagatccagg catataagag
   107281 tgctgcggct actgtatcta catcggttgc tgtagcattg ttgaatccca tcgaattaaa
   107341 gatcgttact agcaaagcgg gcaataatgt aataaagaaa ccatgagcaa taccgccaat
   107401 gatcgctcct cggcgtccgc caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa
   107461 gaaattggtt agcataccag gtaaaatcat cgctaagccg aacatgggca aagtaaacat
   107521 ggcaataatc gtaccaatcg ttgtcgtaat gaaacctaaa atgacagcat ttgggctata
   107581 aggaaagaaa actggacaat ctaaggcagg gatcgcatca ggaactaatt tcatagctat
   107641 ccctcgaaat gccggcacga tttcacctaa cagtagacgt acaccagcta gcaatacata
   107701 cacacccact acgaattgga tggcttgtaa aaaggcaaac atgatatagt tttgatctcc
   107761 tgataattca gaagcgaatg gttcgcctgc aaataaaaca gtgatgatat atagcgggac
   107821 catcacgacc atgacagaca aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt
   107881 gatatcttcg atcgactttt tattttcgcc tttctcacca aaaagcttag caacaccggc
   107941 ttcaaataag tacccgatcg tacaaaaatg acctaaagca atatcatttg atccagtgat
   108001 tttacgaatg atcggttgcg ctactgccgg catagcaatg gcgaaacaag ctccgataaa
   108061 accaccgacc aagatcaata cgatccctcg cagaccggca aaatagccaa agactgtcgt
   108121 catcgttgcc atccataaaa ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa
   108181 acgagccaaa ataatattga atacaaaaat ggctaagaac gtcagtgcaa tatctctacc
   108241 taaacccaaa tcattcattg cttgaccatt gatagattca atagaaggga tgatcccttg
   108301 catatgaaat ccttcagtaa agattttccc gaaataagtt aaacttccaa cgatgatgct
   108361 cgaaccggca ctcaacactt gaaatcctag gagtgtcttt aacgtgccag agatgacttg
   108421 tcctgctgat ttcttctgca gtagtagacc caacatagca atcaatgcaa tggtgatcga
   108481 tgcctgagtc aaaatatttt cgatgataaa attgataacg cccatgtttt gatgtgcacc
   108541 ccaaaagtta gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt
   108601 gtttttaaga aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt
   108661 gcacataaat atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat
   108721 catggttggg aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat
   108781 gttatagaat atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat
   108841 atcggttcaa atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct
   108901 tcacttgaga gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct
   108961 aaaaaactta aagatgagtc tcttgaagaa gaagttattc gtttaagagc ag
//
LOCUS       DAHUVV010000002        90803 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.002, whole genome shotgun sequence.
ACCESSION   DAHUVV010000002 DAHUVV010000000
VERSION     DAHUVV010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 90803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.002"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            12..590
                     /locus_tag="NK767_000096"
     CDS             12..590
                     /locus_tag="NK767_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693122.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(830..913)
                     /locus_tag="NK767_000097"
     tRNA            complement(830..913)
                     /locus_tag="NK767_000097"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(877..879),aa:Leu,seq:gag)
     gene            968..1147
                     /locus_tag="NK767_000098"
     CDS             968..1147
                     /locus_tag="NK767_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693123.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1296..2513
                     /locus_tag="NK767_000099"
     CDS             1296..2513
                     /locus_tag="NK767_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCH6693124.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2697..3533
                     /locus_tag="NK767_000100"
     CDS             2697..3533
                     /locus_tag="NK767_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6693125.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3609..4757
                     /gene="nagA"
                     /locus_tag="NK767_000101"
     CDS             3609..4757
                     /gene="nagA"
                     /locus_tag="NK767_000101"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCH6693126.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4978..6036
                     /locus_tag="NK767_000102"
     CDS             4978..6036
                     /locus_tag="NK767_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCH6693127.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6040..7074
                     /locus_tag="NK767_000103"
     CDS             6040..7074
                     /locus_tag="NK767_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HCH6693128.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7176..7892)
                     /locus_tag="NK767_000104"
     CDS             complement(7176..7892)
                     /locus_tag="NK767_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCH6693129.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7861..8646)
                     /locus_tag="NK767_000105"
     CDS             complement(7861..8646)
                     /locus_tag="NK767_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HCH6693130.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8637..9386)
                     /locus_tag="NK767_000106"
     CDS             complement(8637..9386)
                     /locus_tag="NK767_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCH6693131.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9684..10208
                     /locus_tag="NK767_000107"
     CDS             9684..10208
                     /locus_tag="NK767_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCH6693132.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10224..11723
                     /locus_tag="NK767_000108"
     CDS             10224..11723
                     /locus_tag="NK767_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCH6693133.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11811..12413)
                     /locus_tag="NK767_000109"
     CDS             complement(11811..12413)
                     /locus_tag="NK767_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCH6693134.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12517..13473
                     /locus_tag="NK767_000110"
     CDS             12517..13473
                     /locus_tag="NK767_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCH6693135.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13778..14734)
                     /locus_tag="NK767_000111"
     CDS             complement(13778..14734)
                     /locus_tag="NK767_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCH6693136.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14908..15468
                     /locus_tag="NK767_000112"
     CDS             14908..15468
                     /locus_tag="NK767_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693137.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15461..16286
                     /locus_tag="NK767_000113"
                     /pseudo
     CDS             15461..16286
                     /locus_tag="NK767_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
     gene            16296..16913
                     /locus_tag="NK767_000114"
     CDS             16296..16913
                     /locus_tag="NK767_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCH6693138.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16931..17614
                     /locus_tag="NK767_000115"
     CDS             16931..17614
                     /locus_tag="NK767_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCH6693139.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18297..19580
                     /locus_tag="NK767_000116"
     CDS             18297..19580
                     /locus_tag="NK767_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCH6693140.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19596..20483
                     /locus_tag="NK767_000117"
     CDS             19596..20483
                     /locus_tag="NK767_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6693141.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20500..21336
                     /locus_tag="NK767_000118"
     CDS             20500..21336
                     /locus_tag="NK767_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6693142.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21339..21962
                     /locus_tag="NK767_000119"
     CDS             21339..21962
                     /locus_tag="NK767_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693143.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21959..23251
                     /locus_tag="NK767_000120"
     CDS             21959..23251
                     /locus_tag="NK767_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCH6693144.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23610..23707
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23823..24404
                     /locus_tag="NK767_000121"
     CDS             23823..24404
                     /locus_tag="NK767_000121"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCH6693145.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24407..25714
                     /locus_tag="NK767_000122"
     CDS             24407..25714
                     /locus_tag="NK767_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCH6693146.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26130..26618
                     /gene="purE"
                     /locus_tag="NK767_000123"
     CDS             26130..26618
                     /gene="purE"
                     /locus_tag="NK767_000123"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCH6693147.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26611..27735
                     /gene="purK"
                     /locus_tag="NK767_000124"
     CDS             26611..27735
                     /gene="purK"
                     /locus_tag="NK767_000124"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCH6693148.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27748..29043
                     /gene="purB"
                     /locus_tag="NK767_000125"
     CDS             27748..29043
                     /gene="purB"
                     /locus_tag="NK767_000125"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCH6693149.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29223..29600)
                     /locus_tag="NK767_000126"
     CDS             complement(29223..29600)
                     /locus_tag="NK767_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693150.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29856..30584
                     /locus_tag="NK767_000127"
     CDS             29856..30584
                     /locus_tag="NK767_000127"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCH6693151.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30584..30838
                     /gene="purS"
                     /locus_tag="NK767_000128"
     CDS             30584..30838
                     /gene="purS"
                     /locus_tag="NK767_000128"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCH6693152.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30840..31511
                     /gene="purQ"
                     /locus_tag="NK767_000129"
     CDS             30840..31511
                     /gene="purQ"
                     /locus_tag="NK767_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HCH6693153.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31512..33734
                     /gene="purL"
                     /locus_tag="NK767_000130"
     CDS             31512..33734
                     /gene="purL"
                     /locus_tag="NK767_000130"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCH6693154.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33719..35158
                     /locus_tag="NK767_000131"
     CDS             33719..35158
                     /locus_tag="NK767_000131"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCH6693155.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35181..36233
                     /locus_tag="NK767_000132"
     CDS             35181..36233
                     /locus_tag="NK767_000132"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCH6693156.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36230..36808
                     /gene="purN"
                     /locus_tag="NK767_000133"
     CDS             36230..36808
                     /gene="purN"
                     /locus_tag="NK767_000133"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCH6693157.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36805..38340
                     /gene="purH"
                     /locus_tag="NK767_000134"
     CDS             36805..38340
                     /gene="purH"
                     /locus_tag="NK767_000134"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCH6693158.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38356..39630
                     /gene="purD"
                     /locus_tag="NK767_000135"
     CDS             38356..39630
                     /gene="purD"
                     /locus_tag="NK767_000135"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCH6693159.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39752..40666
                     /locus_tag="NK767_000136"
     CDS             39752..40666
                     /locus_tag="NK767_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCH6693160.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40799..42364)
                     /locus_tag="NK767_000137"
     CDS             complement(40799..42364)
                     /locus_tag="NK767_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCH6693161.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42357..43808)
                     /locus_tag="NK767_000138"
     CDS             complement(42357..43808)
                     /locus_tag="NK767_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCH6693162.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43822..44838)
                     /locus_tag="NK767_000139"
     CDS             complement(43822..44838)
                     /locus_tag="NK767_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCH6693163.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45058..46146
                     /locus_tag="NK767_000140"
     CDS             45058..46146
                     /locus_tag="NK767_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HCH6693164.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46200..47726
                     /locus_tag="NK767_000141"
     CDS             46200..47726
                     /locus_tag="NK767_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCH6693165.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47728..48933
                     /locus_tag="NK767_000142"
     CDS             47728..48933
                     /locus_tag="NK767_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6693166.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49190..50167
                     /gene="guaC"
                     /locus_tag="NK767_000143"
     CDS             49190..50167
                     /gene="guaC"
                     /locus_tag="NK767_000143"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCH6693167.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50431..51855
                     /locus_tag="NK767_000144"
     CDS             50431..51855
                     /locus_tag="NK767_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCH6693168.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52316..53425
                     /gene="pdhA"
                     /locus_tag="NK767_000145"
     CDS             52316..53425
                     /gene="pdhA"
                     /locus_tag="NK767_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCH6693169.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53428..54405
                     /locus_tag="NK767_000146"
     CDS             53428..54405
                     /locus_tag="NK767_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCH6693170.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            54430..56073
                     /locus_tag="NK767_000147"
     CDS             54430..56073
                     /locus_tag="NK767_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCH6693171.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            56081..57487
                     /gene="lpdA"
                     /locus_tag="NK767_000148"
     CDS             56081..57487
                     /gene="lpdA"
                     /locus_tag="NK767_000148"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCH6693172.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            57649..57924
                     /locus_tag="NK767_000149"
     CDS             57649..57924
                     /locus_tag="NK767_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCH6693173.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            57930..58697
                     /locus_tag="NK767_000150"
     CDS             57930..58697
                     /locus_tag="NK767_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HCH6693174.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            58865..60694
                     /gene="typA"
                     /locus_tag="NK767_000151"
     CDS             58865..60694
                     /gene="typA"
                     /locus_tag="NK767_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCH6693175.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            60955..62727
                     /gene="recQ"
                     /locus_tag="NK767_000152"
     CDS             60955..62727
                     /gene="recQ"
                     /locus_tag="NK767_000152"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCH6693176.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            62841..63143
                     /locus_tag="NK767_000153"
     CDS             62841..63143
                     /locus_tag="NK767_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCH6693177.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            63146..64309
                     /locus_tag="NK767_000154"
     CDS             63146..64309
                     /locus_tag="NK767_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCH6693178.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            64335..67763
                     /locus_tag="NK767_000155"
     CDS             64335..67763
                     /locus_tag="NK767_000155"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCH6693179.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            67784..68908
                     /locus_tag="NK767_000156"
     CDS             67784..68908
                     /locus_tag="NK767_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693180.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            68898..69335
                     /locus_tag="NK767_000157"
     CDS             68898..69335
                     /locus_tag="NK767_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCH6693181.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            69319..69657
                     /locus_tag="NK767_000158"
     CDS             69319..69657
                     /locus_tag="NK767_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCH6693182.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            69776..70336
                     /gene="rsmD"
                     /locus_tag="NK767_000159"
     CDS             69776..70336
                     /gene="rsmD"
                     /locus_tag="NK767_000159"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCH6693183.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            70333..70824
                     /gene="coaD"
                     /locus_tag="NK767_000160"
     CDS             70333..70824
                     /gene="coaD"
                     /locus_tag="NK767_000160"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCH6693184.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            70814..71857
                     /locus_tag="NK767_000161"
     CDS             70814..71857
                     /locus_tag="NK767_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HCH6693185.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            72016..72744
                     /locus_tag="NK767_000162"
     CDS             72016..72744
                     /locus_tag="NK767_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCH6693186.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            72860..73105
                     /locus_tag="NK767_000163"
     CDS             72860..73105
                     /locus_tag="NK767_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCH6693187.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            73102..73320
                     /locus_tag="NK767_000164"
     CDS             73102..73320
                     /locus_tag="NK767_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCH6693188.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            73456..73995
                     /locus_tag="NK767_000165"
     CDS             73456..73995
                     /locus_tag="NK767_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCH6693189.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            73988..76261
                     /locus_tag="NK767_000166"
     CDS             73988..76261
                     /locus_tag="NK767_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCH6693190.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            76299..77345
                     /gene="holA"
                     /locus_tag="NK767_000167"
     CDS             76299..77345
                     /gene="holA"
                     /locus_tag="NK767_000167"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCH6693191.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(77434..77688)
                     /locus_tag="NK767_000168"
     CDS             complement(77434..77688)
                     /locus_tag="NK767_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCH6693192.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(78088..78357)
                     /locus_tag="NK767_000169"
     CDS             complement(78088..78357)
                     /locus_tag="NK767_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693193.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(78827..79282)
                     /locus_tag="NK767_000170"
     CDS             complement(78827..79282)
                     /locus_tag="NK767_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCH6693194.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(79420..81348)
                     /locus_tag="NK767_000171"
     CDS             complement(79420..81348)
                     /locus_tag="NK767_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCH6693195.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(81545..82378)
                     /locus_tag="NK767_000172"
     CDS             complement(81545..82378)
                     /locus_tag="NK767_000172"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCH6693196.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(82570..83274)
                     /locus_tag="NK767_000173"
     CDS             complement(82570..83274)
                     /locus_tag="NK767_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCH6693197.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(83297..83923)
                     /locus_tag="NK767_000174"
     CDS             complement(83297..83923)
                     /locus_tag="NK767_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCH6693198.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            84249..86624
                     /locus_tag="NK767_000175"
     CDS             84249..86624
                     /locus_tag="NK767_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCH6693199.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(86648..87139)
                     /locus_tag="NK767_000176"
     CDS             complement(86648..87139)
                     /locus_tag="NK767_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HCH6693200.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            87326..88246
                     /locus_tag="NK767_000177"
     CDS             87326..88246
                     /locus_tag="NK767_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCH6693201.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            88400..88744
                     /locus_tag="NK767_000178"
     CDS             88400..88744
                     /locus_tag="NK767_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCH6693202.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(88934..89434)
                     /locus_tag="NK767_000179"
     CDS             complement(88934..89434)
                     /locus_tag="NK767_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6693203.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            89962..90045
                     /locus_tag="NK767_000180"
     tRNA            89962..90045
                     /locus_tag="NK767_000180"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:89996..89998,aa:Leu,seq:cag)
     gene            complement(90369..>90803)
                     /locus_tag="NK767_000181"
     CDS             complement(90369..>90803)
                     /locus_tag="NK767_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCH6693204.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        29440 a        15662 c        19443 g        26258 t
ORIGIN      
        1 tttttttaga catgaatatc ttatttttat tgctattttt cttaattttc cttctaacat
       61 ataggaaatg tcaaaactat tatgtgcgta tattgactgc attaccgttt ttcttaaacc
      121 tgattattta ttttggtaat acaatgggtc aaagctttac ttacgtcaat ggaaataaaa
      181 gggcaatgat ttggagtagt agtaacctga ataatctctt tacagagtta ggaacgaaac
      241 tgtcactttt ttatccaggt acttggatag ctactttagt agtcttggca ttattactat
      301 gtttaattgt tggaatttat ttaagttttg acaataagaa aacttctatt tttttagtta
      361 ttttgatgat aatgggattt tgttcaaggt taattatggg attttctccc acagtatggg
      421 cttccggaat gcggacgtac tatatacttt atgttgttat cgccatctta gtactaatgg
      481 cagttaaaga gctaatgaaa agcatgagtg tacaaaaaaa tgaatttatg caatttggat
      541 taaccgtgct tggtatttgt acctttataa taacggtgat aaacagataa tatacaaaaa
      601 atccctcgct ttagtattag gtgagggatt ttactcgttt ttcgattaat tgttttaaca
      661 cttgcaagtt tctacgagta gcttgtttcc gaccttcata tactcacctc aggctgttta
      721 catgattgta cgttcttttt gtaaaggata cagtgatatg tatataatgg cataaagaaa
      781 cgttcaaaca aaaaaactcg tgaagcaaaa ttaacttcac gagttgtagt gcagatgatg
      841 ggactcgaac ccacacgagc taacgctcac atgaccctca atcatgcttg tctgccaatt
      901 ccaacacatc tgcgtaataa taacacacta atgatagcga ggttttacat agttgtcaag
      961 ctcaaaaatg ggaggactta ttaagatgga gaaatatgac ggagaatttt caggactggg
     1021 aatgatctta ggtgtattga ttggacttgc attcggacgt tttttgcttg gttttatgct
     1081 aggaatcatt tgtggaattg caatggattg ggcagcgaat ctatggaatg actatcatga
     1141 caattaagtt ctcaacgctt gtttgtgttt ttttaatact tacgtttttc ctaaataagt
     1201 ggcaaaattt tctaggatgg agtatgatat tgaaatagta aataacaaaa aatttggaaa
     1261 gttctaactt tttgaaatga gggattggct aagtgatgag agaatttgaa aaatccaaca
     1321 aattagaagg tgtcagttac gatgttcgcg gtcccgttct agaagaagcg gagcggatgc
     1381 aagaagaggg cattagtatt ttaaaattga acacgggaaa tcccgcacct tttggatttg
     1441 aagcaccaaa cgaagtcatt cgtgatttga tcatgaatgc ccgtgactca gaaggctact
     1501 cagattccaa ggggatcttt tctgctagaa aagcaattga gcaatattgc caactgaaac
     1561 attttccgaa tgtgacgatc aatgatatct acaccggcaa cggagtgagc gaattgatca
     1621 cgatgtgtat gcaggggttg ctggacaatg gagatgaagt actggttcca atgccggact
     1681 atccgttgtg gacggcttct attgcattag ctggaggcac acctgttcat tatatttgcg
     1741 atgaagaggc agaatggtat cctgatattg atgatatcaa atcaaaaata acgagtcgga
     1801 caaaagctat tgttatcatt aatcctaata atcctactgg ggcactttat ccaaaagaat
     1861 tattagaaga aatcgttgaa gtagctcgac aaaataacct gattatttat tccgacgaaa
     1921 tctatgatcg cttagtgatg gatggtctag aacatattcc gattgctaca ttagcgcctg
     1981 atttgttcgt tgttacgctg aacggtctat ccaaatctca tcgtgtagca ggatttcgag
     2041 taggctggat ggtcctaagt ggagataagt cacacgtcaa aggctatata gaagggctga
     2101 atatgttgtc ctccatgcgt ttgtgttcca atgtattgtc tcagcaaatt atccaaaccg
     2161 cgctaggcgg ttatcaaagt gtcgatgagc tgctcctgcc aggcggaagg atctatgaac
     2221 aaagagaata tatctataat gcaatcaatg atattcctgg tctttcagcc gtaaaaccaa
     2281 aagctgcttt ttatatattc ccaaaaattg atacaaagcg attcaatatt ttggatgatg
     2341 aaaaattcgt tttggacttt ttacatgaac atcatattct acttgtacat ggtggtggat
     2401 ttaattggaa ccagccagat cattttcgaa tcgtttatct tcctaaaatg gatgatttga
     2461 aacaaacagc taagaaaatg agagaattcc tagcaaccta tcgccaaaaa tgattttaaa
     2521 aaccaaattt ttcagtagga gggccacaga caaacacact gaggctctct ttctagtgga
     2581 gttttcttaa ataactaaag tatattattt agttgcaagt cttttcttga agtttgcgac
     2641 ttcctaaagt aaaatacgaa tagaatgcga atacgcatat tttggggagg atacttatgg
     2701 aaacgttaat cgagtccttt ggaacgatca ttaaagagat tcgaaaagaa caaaaaatga
     2761 cgcaaaaaat gctttcgcat gatatttgtt cacaaagtgt attaagtcgg atcgaaaata
     2821 atgaggaact gccgaatgta ttggtgatga tgcagatttg tcaacggcta ggggtaacga
     2881 ttgatcatgt gatgaatctc tctcgaaaaa ctactcggac aaacgataaa ttatttgaac
     2941 tgttggatag ttgtttccaa aaacgtgatt atcggaagtt agaacaagta ctaaaatctt
     3001 ctgatatttc tgaaaatctg tatcaagcaa ctgactttca acgctattat tattatctcg
     3061 gtgtttgcga attcacgttg cgtcaaaata tctctcagtc cctttattat ttgaaagaag
     3121 cattaagcta ctctagccaa aaagaaagaa tccatgtttc tgacacagaa atacaactga
     3181 ttagttgtat cggaaaaata tatggtgtgg caggaaaaac ggctgaagca cgctactatt
     3241 tgagtcgaag catccaactg ctgcatcaat cagctgatga acgatcaaaa agtgaatttt
     3301 cgcaaatttt ttataattat ggaaatttct tattccatca aaatgaaatc gacgaagcgt
     3361 tagaacaggt gaatcaagga atctattggg ctcaagagaa gaatagctac tattacctca
     3421 atgatttatt tgtgcttaaa agcttaatat acaagcgtaa agaagaacca gaaagagcag
     3481 cattctatga aggcttagca caagctgtga aacaaatttc aaaaaatttg taaatggtgt
     3541 agaccattct tgacttcttc tctaaataag agtactttag aggtagaaaa acaaagggag
     3601 agaatagaat gagaactttt atctatgctg acaaattctt tttagcgtca ggtgtcaaac
     3661 atgcaggcta tcttgaaatt accgatggac tttttggtga atacaccaaa gaactacctg
     3721 agggagaagt caacatcatt gaccaaagcg gcaaatggat tgctcctggt ttggtagata
     3781 cacatattca cggctatatg aatcatgatg taatggataa tgatgcagaa ggcatcaaag
     3841 tgatgtctga aggtctgtta tcttgtggag tgacttcatt tttaccaaca acattgacgt
     3901 caagcaaaga acgtttaaaa gatgtagctg agacaatcgg caaaatgtat caggaagcac
     3961 caggagctaa aatcaaagga atctattttg aaggtccttt cttcacagaa gaacacaaag
     4021 gggcacaaaa cccaagttat ttcggggatc ctgatttgga tactttccat gaatggcaag
     4081 aagcttcagg tggtttaatc aaaaaaattg ctttagcacc ggaacgtcaa ggtgtagaag
     4141 agtttgtcaa acaagtgact gaagaaggag tagttgtttc tctaggacac agtaatgcta
     4201 cactagaaga agcgcaaaaa gctgttgaag caggcgcaag tgtatttgtc catgctttca
     4261 atggtatgcg tggattgaat catcgcgaac caggtatggt gggtgcgtta ttatctttag
     4321 aacatgtctt ttctgaattg atttgtgatg gccatcatgt acatccaaat gctgcgggaa
     4381 tcctaatgga aaaagcagga catgatcatg tcgcattaat tacagactgt atgatggccg
     4441 gtgggatgcc tgatggaaat tacaatttag gcgaatttcc agtagtagta aaagaaggaa
     4501 cagcacgtct agaatctggc aacttagctg gaagtatatt gaaattgaaa gaagcaatta
     4561 aaaatgttgt agactgggga ttagcaacac cagaacaagc agtcatgatg gctagtctga
     4621 ttccagcaat cagctgtaaa atcgatgata catgtggaaa aatcgctcaa ggacgcgatg
     4681 cagactttat cgtattgaac cctgatatga cattggaagc aacttatcta gatggcgtgg
     4741 aacgtttcca cgcataatat aatttaagtt aaccttaaaa ctaaacttct taagtttggt
     4801 tttaaggttt tttttgtgtt ctagtaaaaa tcactttaca taagtttcaa aagtttcttt
     4861 aatttcctat tgttcaataa gtaacaatgt aaagataaaa ttaaaaatgt taatacgtat
     4921 tagcaaaata aaagcttttt ttattgtgaa taaatgaaca taattaggag gagaaagatg
     4981 aacttatttg acatttttcg atcgactttg acagatatgt cgattgtcag tgcaatcact
     5041 tcaacagttt ttattatatt attagggttt gtttgtagaa aaaaggggat tttttctgaa
     5101 tctgttggaa aaattctatc gaaagtcgtc ttaacagtgg ctttgccagc actggcattt
     5161 cgagctttca tgcaagatat caatacagaa ttactgatgc aaggaatcaa tgtcttgatt
     5221 tggggattag ttatttatat tattcttatt ctagtttcaa aacctttttt cttaagctat
     5281 aaaggggata agcaaacaac gttgcgtgtc ctgacgatct ttggttctac gacctttttc
     5341 ggaacgccta tcgtcagtgc gatttatggt cctgtcggcg tgatgtattc ctcaattttc
     5401 aatattggct accgaatctt tttgtattct tatggataca taaaaatgag tggcttgaag
     5461 atggaagcta aaaatatcaa agcgatgttc gtcaatccaa tcattatcgc tacttttgcc
     5521 ggccttttca tttggctgtt ccaagggtat ctgccacaag tgtcagttac aggtgcagaa
     5581 ggagagataa gccaagtagc gtttttgcga attgaccaga ctgcaccgtg gctatttcag
     5641 ccgatggatt atttagctgg tttaagttca ccattggcgt ggctagcaat cggcgcaaca
     5701 ttaggtgaag taagcttcaa aaaagctgca tctgaaaaaa cttcatggta ttacagtcta
     5761 gtcaaagtgg tatttgtacc tgcattgaat atcgtgattc tagcagtttt gacggcaacg
     5821 aatattctac cagtcaattt tgaagcactg gcaacaatcg ttatcatgat ggctacacca
     5881 gctgcaacag tggcagcagc ctatgctatc agttttgata gggaggcact tttagcctcc
     5941 aatgcatcct tactgtcaac tatttttgct gtggtactga cacctgtatg gatcgttgta
     6001 ttacaaatga ttcatggcat aggtttattt tcataagaaa tggaggaaga aagtatgaga
     6061 atcgcatgtt acggtgttcg tccgaacgag atagattttt ttgaaaaatt gaatatttac
     6121 cattatgatc tcagtcttta tgaagaactg ttgacacatg acaatatcgt gtcagcaaag
     6181 gaccatgatg ctgtattgtt gaggggaaat tgtatagcag atgagaccaa tcttgcaaaa
     6241 atgcaggaat atgggattcg ttatgttttt acacggactg tgggagtcaa tcatatcgat
     6301 ctacaagcgg cagctgattt tggtatgaca gtagcccgtg taccatcgta ttcgccaaat
     6361 gcgatagcag agttatcttt gacttttgcc atgatgcttt tgagaaatac tgcgtatacg
     6421 acgatccgaa catctttcaa agattttcga gtggatgagc aaatgtttag tcgtgaaatc
     6481 agaaattgca cagtagggat cataggaaca ggcaggatag ggttgacgga agctgttttg
     6541 tttaagggac taggagccaa agtgatcggc tacgatattc atcagacaga agctgctaaa
     6601 aaagtattga cgttccactc gcttgaagag ttattagcca aaagcgatat tgtcagtatt
     6661 catgtacctc atatcccagg tgaaaacgat caaatgatca atgaaggatt tttagctcaa
     6721 atgaaaaaag gtagcatctt gattaataca gcccgtggag agcttcaaga taatgaagct
     6781 attttaagag ctttgaaaag caatcattta gctggttttg caaccgatgt ttttgcaaat
     6841 gaaacagagg tttttttccg atccttcaag ccatgggaga cgattccaga ccctgctatc
     6901 cagcaattag tagaaatgta ccctagagtt cttgtaaccc cgcatgtagg ttcaaataca
     6961 gatgaggcat tatcaaatat gatttcgaca agtttcgaga actttcgtga aataatagaa
     7021 actggaaaaa cgaaaaatga ggtatctctt cctaaagccc gtcaattaag ataagaaaac
     7081 atggatattc gtcaataaaa tgtttaaaaa tagtccaagt aaacagttgt tacttctgta
     7141 tacttggact attttgtatg ggataattcg atgactcaac agtaatctgt gagctttttc
     7201 cgcccattct ttgtcaattc ataacaggtt agcggccggc cgatttccaa atatttgatt
     7261 ctttcgttca atatatcttg ttctactaaa aaattcaaat atttcttgac ggatatacgt
     7321 gacagtgagc ttttttgagc cagttctttt gttgaaaaaa tagtatcttc ttttagaata
     7381 aatgcactta ttttttttaa agtcagtttt gacagacctt taggcaaatc gatgaatgat
     7441 ggtatttcgt gaatgggagt ggtgtggttg aatacttgat cgatcaattt ctgatcggct
     7501 ttttcagtcg tttggattgt ctgagagaaa gcaagatact tttcgatcgc cagctgaaaa
     7561 cgttgcaaag tgaacggttt gatcaaataa tctaaggcac cgtagcggat cacattcttt
     7621 atggtttcga tatcatctgc agcagtgatc aaaatcactg ggatatcatt tccttgctgt
     7681 ctaagccaag ataaaaaatg gataccactt tcgccaggca tgtatacatc aagcagcagc
     7741 agatctgcgt ttttttgtct gaaatatttt tttccttctt ctacatttct gaaactttcg
     7801 attacgttca tattagggat gctttccaca aaagtacgat tcaattctgc aaccattgga
     7861 tcatcttcga ttatcaggac atttgtcatc attttgatct tccttttttg gtaattctag
     7921 atagacgatt gtaccgccat ttgtttgttt ggaaaattct aaaaatccac cgtggcgttt
     7981 gacgattgaa tggatgagat tcaatccaaa cccacgattg attcccttcg tcgaaaaacc
     8041 tagcgtaaat attttttctg agatttcttc tgatatcccg acaccgtcat cgcagacggt
     8101 cacgatgaat acttcagctt ctgtgtcata gtttaaatga atccggacct gtccgtttct
     8161 ttcatgtttt tgtagcacgg cttcttttgc attatcaagt aagttcccta acacttgcaa
     8221 aagtgaatgg atggagtcat ccatctccat tttagggata aaagaatcgc tttctaacag
     8281 catttcaaca gattgttctt tggcttcgtt tattttgcct aataaaaaac cggcaattgc
     8341 aggtactttg atcagacttg agacttggcc aatctctttt ttataatttt gatttagctg
     8401 ctggataaaa tgggtgactt cttcatactt cttcaaatca atcaaaccag aaatgacatg
     8461 aagtttattc atgaattcat gggtttgagc tctcatggag tttatatatt gtttcgtccc
     8521 cccaagctcc tccattaatt tcattacttc tgatcgattt cgtaaagttg cgactgctcc
     8581 taaaaaacgg tcattgctaa aaagaggtgt aacgctagaa atagtttcta tcccatttaa
     8641 atacatggat tgagtccgaa cggtcgtttg atgctcaatc gtctctttaa acaaaacgtc
     8701 atatagatta tcactgattt ttttcctact tcgaaatcca atagctgctc tgtttggtag
     8761 aaaatgtttt ttgcaggtgt attgatcaag aggatagttt tatcagaaga gatggctaag
     8821 attccttcat ctacttcatt ttcgataatc gttttttcac ttaactgttc tgctatttct
     8881 tgtggttcaa gtccaagcag tacttttttc attttttggg caagaaaaac ggcgcctatg
     8941 atgcctacgc taagtccaat aatcaatgaa aaaacaatca ttttttgtcc atctttgact
     9001 tgattgtcaa tcgtgttgtt tgttaagcca acacaaactg caccaataat ctcttgatca
     9061 taataaatag ggacgaaata gcggaagccg aaccctaaca ttccttcttt ttcagagaaa
     9121 aattcttgtc ctccaatcac ttttttcaca tcttcatgac ttgagaaagg aagaccgatg
     9181 taacttgggt ctgcgtgaga atatcgaatg ccttcagggt ccatgacaac gataaaatct
     9241 aaattcgatg caatcatgat ttcatttgtt attccttgaa tcgcatgtgt cgggtcacca
     9301 gtctgcagct ttttttggat gagtttatca ttggcaacga ttttagcaat gctagagatt
     9361 ttatctgaca cactagatcg gttcatcgaa agaataaaat tcctgatcaa tatggcagaa
     9421 acaatcaaag aaacgaatgt ggagaaacaa acgacaagga tgatcgtgtt ctggagcttt
     9481 aatggttttt tcttaaaaaa aggaaacata ggctacctcg aaagaattta ttgtgaaaat
     9541 tttaacatga aaacaaaaat ttaacaaatt gtcaacgata aacacaacct tcctataaaa
     9601 aacaccactg agtcggtaaa agaagctata cagaattttc aaacattggt atgatagacg
     9661 tagaaatgga aggaggcgtg tatatgatag ggggaatcgc acttgagacg atttattggt
     9721 atacattgct gatttgtgca ggattggcag tcctattgat tatttttgga gatgtattcg
     9781 attttgatgg cccgcttgat ccgatgctta ttatcccttg gttaactttc acaagcttat
     9841 ttggttttct aggtgagcgt ttaacagatc aatcatcttt atttttattt attgctagtg
     9901 gaggcatatc gatagtgttg gtatttttgc ttaatttcta tatattgatg ccgatgaaac
     9961 atgccgaatc aacgatgtcg tcttctgaaa aaactctgga aggacaagta gctgttgtga
    10021 tcacaccgat ccctgtacga ggtatgggag aaattcagtt gaaaagtgtt accggttcca
    10081 ttagtcgtcc agcttgtttt tacagccccc aagaacagac agctgagcgg ggaacgaaag
    10141 tattgatcat tgagatcaag gaaagaatct gttatgtcat gccttatgaa agcatgataa
    10201 aagcttaaag gagcgtgtgt taaatggaaa taccagttgt ttttatcgcg atcgcaatcg
    10261 cagctttgat tttactgatg ttgctgatcg tctttgtagc gaagtaccag acagcaaaac
    10321 cggatgaagc gttgatcatc agtggaagtt atttaggaag caaaaatgtg cacattgatg
    10381 aaagcaataa caaaatcaaa attgtgcgtg gaggtggcgc attcgtcttg ccagtttttc
    10441 aacgttcgaa tcggatcagt ctattatcga gtaaattgga cgtctccacc cctgaagtgt
    10501 atacggaaca aggcgtaccg gtgatgtgtg atggtacttc gatcatcaaa atcggttcat
    10561 ctgtagaaga gattgctaca gcagcagaac agtttttagg caagacaaga gaagaactgg
    10621 aaaatgaagc aagagaagta ttagaaggac acttgcgctc gattcttggt tctatgactg
    10681 tagaagaaat ctatcaaaat cgcgataagt tcagtcaaag cgtacaagaa gtcgcttctg
    10741 tagaccttgc aaaaatgggg ttgatcatcg tttctttcac tatcaaagag gtcagagaca
    10801 aaaacggtta tcttgactct ttagggaaac caagaatcgc ccaagtcaaa agagatgcag
    10861 atatagcaga agcggaagct ctaaaagaaa cgcgtatcaa aaaagctcaa gcagaaaaag
    10921 aatcacaagc agctgaatta cagcgacaaa cagagatcgc agaatcatta aaagaaaaag
    10981 aactgaaatt agcaacctac aaacaagaac aagatgttgc taaagcaaaa gcagaccaag
    11041 cctacaattt agaaagtgcc cgagcacaac aacaagtaat cgaacaggaa atgcagatta
    11101 aaatcgtaga acgtcaaaaa caaatcgaac tggaagaaaa agaaatcaca cgtcgtgaaa
    11161 agcaatatga ttcggaagtg aagaaaaaag cagatgccga tcggtacgct aaagaacaag
    11221 aagcacaagc tcaaaaagtc aaagaagtga ctgaagccga agcagaacga ttccgtgtgg
    11281 aagcactggc agaagcggaa gccaataaga cacgtttagc tggacaagca gaagccgaag
    11341 cagcactagc aaaaggaaaa gcagaagccg aagcaaaaca aaagatcgcc aatgccttca
    11401 aggaatatgg cgaagcagct gtcttgagta tggttatcga tatgctaccg caattgatgc
    11461 gcgaagcagc acagccgcta ggaaatatcg aaaaaatctc tgttgtcgat acaggaagca
    11521 gttcaggaga aaccagtgga gcgaaccgag tgacaaatta tgccacaaat ttgctttctt
    11581 ctactcaaga aacactaaaa gcgacaacag gacttgactt gaaggaaatc gtagagaatt
    11641 tgtcgacacg tgggactact agccaagtaa atatgtatga agcgccaaaa gaggcagaac
    11701 aaaactcgat gaaggaagaa taaagtagta cacttcaccg ttcaatcaaa aatggaggaa
    11761 gactttttaa agttcttctc tccatttttt tattttattg caaaagaaaa ttagtcctcg
    11821 ctatttttct tttgtttttg gatcttagct tctatctctt gtaaatttgt tcttagcaat
    11881 cgttctttct tttggagttc gaccaaatct tctgatagtt cttccttctt ttctgtttca
    11941 ggaggacgaa gaaagaaatt gataccgatt gcaatgaatg tccctataaa agtatctagt
    12001 acacgttgga tagcatacaa agcggattcg ccttgaggga tgctcaacgc aatcagcaac
    12061 aacgtagcta ttgcggaaat gataccggaa ttattgttaa taccatctga cacgacgatc
    12121 acaataataa ctagaatagg aagcaagatc aattctacca aaaaatcatt gtgaaaatag
    12181 cttttgacaa aaaaataaat gatagctaat gtaccaccta atgtattgcc gatgatccta
    12241 gactttccaa aagaaacggt tgtagtcaag tcctgacgca aagaaaaaac agcagataat
    12301 gcagcaatca tcgggattcc tcgttgagtc aaatgaaaaa acaagataca taaaaaaaca
    12361 gctaaggcac ttttgatcgt acgcatacct agtcgaaaac ggccaaattg catagagatt
    12421 ctcctttttg gtctttttac tatcatacct aaacttttgt ctgatagcta gtttttcatt
    12481 attcaagtat aattaaaata aaaaaggggg aagtaaatgc gtataggaat gcgaacagtc
    12541 aaaacagtga tcagtgtctt cttttcgatg gtcacagcgg gtagcttatc gctgttgtat
    12601 tggccagctg cagggatcat tgcattatta agtgtaggaa ataccaagcg ctctacattg
    12661 cgtacgggca tgaatcgaat cgcagccttt atattagcta cggcaacagc actgttcagt
    12721 ttttttgctg ttgggtatac tatttttggc ttcagtcttt ttttattcct gttcatcccg
    12781 cttgcctctc gcttcaaatt aaccgaaggg atagtagtaa attcggtttt ggtcactcat
    12841 tatttagtgg aggaaaacat gtcttggcag ctgatcggca atgaagcggg attaatggcg
    12901 attggattag tatttgcttt gcttgcaaat gtttacatgc cagatcgtac gaagcagctg
    12961 aaggaaaacc aaatacagat cgaaaaagaa tttcgaaata ttttgagaca aatggcagaa
    13021 tttcttttat cagaaaataa agaagatgtg cagatcaaat gcgaacattt actgacattc
    13081 attcgcgaaa gccaagaaga tgccagagaa catcaagaaa actactggct cagacagccg
    13141 ctatactacg aaacttactt ctctatgaga agagcacagg ccaatgtgat taaagacatg
    13201 ctggaaaatc tagagagaat ccagcaacca gcttattatg gaaaacatat ctatggttta
    13261 ttgatttata ctgcagaaac attttcagag tcaaatgatg gcagacaaat tttaacacgt
    13321 attgaagaag tttatgtatt gtatcggcaa atgcctctgc cgactagtcg ccctgaattc
    13381 gaggatcgag cagaattgtt ccagtttttg cagtctttta aaagttttat tgaaatcaag
    13441 gcagaattct cccaacaaaa aatccaaaaa tagaggttgt gaattcagct aatattcagg
    13501 tcaaaaagct gatttcacaa cctcttcttt aaacggtgtt ctcctgtcaa ctcctcggtc
    13561 acaagtcaca atattcaaca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
    13621 ggagctgaac ggtaggctca caacctcttt cttaaacggt gttcaaaagc tttcctctgc
    13681 ggtaataagt ccagccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatt
    13741 acttgatgca gaacagcttt ttcacaacct tttaaaatta catatttttc catagaactt
    13801 gcatttgatc caagatttcg atgacatctt ttgtcaagaa gagagagcga ttgggcaagt
    13861 caccttcaat catgtgtacc atattcttta tttcgtaatt cattgcttca gatgtattcc
    13921 cgctttcgat gaattccttt gtaccatctg taaataagat ctccgcacga tcagcacgcg
    13981 ggtaatcagc aatcgtcaca taaccgttct caaaggcaac gataccgact tttggcattt
    14041 ttgcctgaaa agtcaagctg acagcagcaa gttcattttc tttgttacgc aagatagtga
    14101 cggattgttc gtccacgcct gtttcaaaag gaaccattgt agaggcaact acttcaggtt
    14161 gagagctcaa aaagaaacga gcgaaagata cagcatacgt accgatatcc aacagagctc
    14221 ctccagctaa ttctggattg aagaaccggt ttttagggtc tggttctttg taactgccga
    14281 aaggtgcttg gatcattttt aaagccccca gttttcctgt atccattatt gagcgtaact
    14341 gctgataaag tggcatgttg aagattgtca tcgcttctgc taagatcaaa ttcttttctt
    14401 ctgctaagcg catagcgtca gccagttctt ttttattcat cgtgatggct ttttcacata
    14461 gaacgtgttt gcctgcttcc aatgctgcca agatatggtc gatatgctgt ctatttggta
    14521 cagcaatgta cacgatgtcg atttcagcat cgtctaacat ttcttgatag cttccgtaag
    14581 cttttggaat attgtaacgg tgagcgaagt tttccgcttt ttctgaagtt ctggaagcaa
    14641 ctgctgttag ttcgctcgtc tcttgattga agctttcggc aaactcatgt gcgatggtac
    14701 caagaccaac gattccccat tgatattttt tcatctgaaa acgctccttc atagttggat
    14761 tgagtttctt gtctgtctat tattataaca aaaatcacga agaataaaga ggagaggact
    14821 tgaaacatta aaaaaaatga aagcagcgat ttaagaggtg ctttttgatc aaaacttaag
    14881 tagaatagtt aaaaggaggg gataaacgtg catcaagtat atattgatct tattatgagt
    14941 gtattattag aacagtttga aactgaaaaa aattttttag atgaatactt gctgatcagt
    15001 ccagaacaat ggcaaaaatg gaaagatggc gagaaaaatc tgccatctga agcgatgcag
    15061 aaagtaaaaa atcttttcac tgattatgaa tggatgctaa cgcaaaagat tttgcgtcaa
    15121 acgattattt ttcccgaaaa acgaaacgtt gccgttgcag agtacaagca aatcaaaaca
    15181 cgcatcgcgc aaaaatggct tagcagcaaa gcagcaaaag tcgagctgat ccctttgaaa
    15241 aataatcagc aggctggtag ataccttgac ttgaaagtaa gtattcaata tgatgaatgg
    15301 ggctatgatg atattttgaa ttttcgtctg ccagcagatg ttcaaaaaca gatcgaagga
    15361 gaaaaaatcg aacttctcga ttgggtgaat gaaaatctgg aagaaactta cgttcatgag
    15421 gcgaatgggg aacgtgaaga acaacgaaag gatttttcaa gtgaataaat ttttaggatt
    15481 tggagcagtt gtgattatga tcgcagcaat gggctatgtt atttttagcg atgatgattc
    15541 gcatgttcaa gccggcaatc aagcagtcga acgaacaagc accaaaacaa ctgctagtaa
    15601 agaaaagaac agtcaaacaa ccagcagcaa ggtaaaggac aaagaaacaa aagatcgtac
    15661 gtccaaacaa ttgccagatg ttaaaacaga cgattggaag ctagttttgg ttggtccaga
    15721 tcacaaatta gaaaaagaaa ttgatgagtc tactcaattg gcatcattaa gtaacggata
    15781 cttgatcgac aaacgcattg ctccagaata tgaagcattt gccaaggctg ctgaagcagc
    15841 tggatttcca ttagtgatgg tctcagctta ccggtctgtt atcatcccaa caacaagttt
    15901 tcgaacaaaa tgtccaagat gtgatgagtc gtcaaggtgt atcagaagag gaggcgacaa
    15961 agattactaa agaaacgatt acggagccag gatacagcga acaccacaca ggtctggcag
    16021 tcgatgtcgt cgatcaaaac tggtacaaca gttatccttc tactgtattg gatgcaagtt
    16081 acggagatca gcctggagct aaatggatcg cagataatgc tgcgcagtat ggatttatcg
    16141 ttcgttatcc aaaaggacac gaagatataa ctaagatcac atatgaacct tggcacttac
    16201 gttatgtcgg taaagaaaac gctgagtata tcaccaaaca ccaattgacg ctagaagaat
    16261 atctgaatga attaaatgaa aagtaggaat tgatcatggc gaaaaaaaag tggaaaaagc
    16321 aaaagcgcaa gaatatccgt tggccagcag tggttgccgg tgtagcagtg atactcttag
    16381 cctttgtttt ttccttgaag aatctgtctt ctccttttac tgataatcag caagaaaatg
    16441 aagcgcaatc ccatcagcaa tttattgaac ggcttgttcc gcatgcccaa gaactacaag
    16501 atggttatgg agtgcttcct agtatcattt taggacaagc tatattagaa tcaaattggg
    16561 gaaaaagtac cttggctagc aaatacaata atcttttcgg gatcaaggct tatggggatc
    16621 agaagaaagt aagcctggaa acaaaggaat ttgttaacga agaatggata actatccaag
    16681 gagacttcaa agtgtatgat tcatgggaac agtccatgga tgaccataca caactgttcg
    16741 tccaaggagt agattggaat cctgcgctgt atgaaaaagt gattacggcc accaactatc
    16801 aggaagccgc acaagcattg caagatgcag gttatgcgac agaccccggt tatgcgcaaa
    16861 aaatcatcca ggtcatcgag acgtatcaat tgaatcagta tgaccggaga tagcaaagga
    16921 gaagtggctg atggcagaac aatttacgcc agaaatcatg acggaattga gaaaagatat
    16981 cgtaagagta ccggaaatca ttttagaagc aagcgggatc aagatatttg gaaaattgat
    17041 ccgttcgatc atctttacta cggatattgc gatcatacgt aatacaaatg cgaatgcagt
    17101 aattgctgtg tatcctttta cacctcatcc agcgattact aaaagcatta ttgaggcagc
    17161 tgacattcca gttttctccg gtgtaggtgg tggtttgaca caaggtgctc gttcttccta
    17221 catgagcatg ttcgcagaag cgcaaggttc gttaggggtc gttttgaacg gtcctacgcc
    17281 tttagagaca gtaaaagaag tttgtcgagt agttgatatt ccagttatca gtacggtaac
    17341 gtcgaaatat acagaaatag atgaaaagtt agcagctggt atttcgatta tcaatatcag
    17401 tgcaggtaaa gaaactgcag aagcagtacg ccattttcgt aaaaaatatc ctgaacttcc
    17461 tatcattgcg acaggtggtc caacggaaga aagtattgct gagaccattc aagcaggagc
    17521 aaatgccatc acatatacgc caccaagtag cggagagctt tttagtcgta aaatggaaaa
    17581 atatcgggaa actgagaaaa agaatcatga ataacaggtg agaatcaaaa gacgaggaag
    17641 agcagatagt ggctcttcct cgtcttttgt actccagtac tatttgttca gaattcaaaa
    17701 aaaagagaaa tttttttgtt tttttacctg taatgtacat aagaagagtt aaaacctttt
    17761 gttattatta gcattctaac aaaaaaatgt cttgatttat tgaagcgctt tctatagaat
    17821 gaaaaacgaa taaaaactaa aaaaggcacc cgacaattta gtagcagcta aattgtcggg
    17881 gcttgaaaag tcagcgggaa ctgaccaatc aagttgccat agtcaatgct tgtgcatcga
    17941 cgtgtatatt gtacctaatt tcatacagaa ttactagtta tttttctgtt ttttagggaa
    18001 gagcgcacac gaagaagcat gcttatttag ctatgtcaag gatattgggc agttgtctga
    18061 tatccttttt tctttgcgta attatcctcc catcattgaa gcaagaagtg tttttagcga
    18121 tgaaagaaga atcagcacta tctaattttt cttgaaagta ttgagttgtt catcctaggc
    18181 agaacgataa ggcaagtctt ggtttttatt ccccttgaaa aagatcgttt aataaatatg
    18241 ttctgtataa aaatacagta tattgaaaaa taacaatgga agtagaggtg cagagaatga
    18301 agaaaagaat gagaatcatc ttagctttaa gtgttatgtt attcttcttg acggcttgtg
    18361 ggcaagaaga tgacaaaaaa atcacgctga acatggcttg gtggggttcg caagtccgac
    18421 acgatgcaac agtaaaagta atcgagcttt atgaagaaaa aaatccccat gtgacgatcg
    18481 attatgaatt ttatgattat gaaggatatt acacaaaatt aggtacacta gcagcatcag
    18541 atgatatctg ggacattttc caactgaaca atcaatttcc tcaatacatc aatcagattg
    18601 aaccattaga tgaatatgtg gagcaaggga taattgatat ttctaatgca gatgaatcct
    18661 atttagcaac gacgacttac gacgggcaat tagttggact gtctaacggg gtgaattcgt
    18721 ttgcgattgc ttacaataaa aatatatttg aacaattaaa tatccctgaa ccagcttcaa
    18781 attggacatg ggatgaattt gaacaaatca gctatcaaat cactgaagaa ttaggaatct
    18841 atgctatttc aagattcgaa gattttatag ctggctgtat tattcaaata ccacaagttg
    18901 agaaaggatt gaatttttat aatcgggaag atctatctaa cagcttggga tttgaaaatc
    18961 cagactatct aacagatttt ttccagctgc gtaaaaatct ggtagacaaa ggggcccatc
    19021 cagaaccagg tggtggagct gcaagtacga cggatctgtc ttttcaggcg gttagggata
    19081 gagaagcggc aatggtgttt ctatcaagta accagttgac agaaatattg tcaggggctc
    19141 ctgaagatat ggagattcag ttgattaatc cacctcgcag aaaagcagat ggagaatctg
    19201 gtatcccttt acgttcttcg caaatgctga gtatggcaaa aagctcagcc cataaagaag
    19261 aagctgctaa gtttattgat ttctttcaaa acagtgaaga agccaatgaa attttaaaag
    19321 gagaacgtgg tgtaccaatc atgtcgaata ttcgagaaca gctcgctcgt gcagatgatg
    19381 caactttgca agcgacattt gattatttag aaatgatttc cgatatggac aacggcgaag
    19441 tgaatgtctt tgatagtgct ttgaatccgg aaattcaaga ccaatacaat cttttagttg
    19501 aaaaagtcat ttatgaagaa gtaacagcag aagaagcagc agagagtctt tatgagtttg
    19561 cgtcaaacat tctaggatag cagcaggagg aaggcatgaa aaaagaaaaa ctaaaacggt
    19621 tcttggatca agacaatgtg gtcggttacg tatttgcttc tccattcatc ctaggatttc
    19681 tattgataac agtgataccg atgatcttgt cactttatta ttcgttcacc gattatcgtt
    19741 tgggaaatcc agtctcatgg atcggcttgg ataattatat aaagatgctg agtgatgccc
    19801 gttttttgaa ctccatccga gtcacttttc tctatgtatt tgcatctgta cctgtgaaac
    19861 tgatctttgg tctattgatt gcttatcttt taacgaggaa tatcaaagga acgactttct
    19921 ttcgttcttt gtattatgtg cctagtttga tcggtggcag tatttctatc gctctagttt
    19981 ggcgggagtt gttttctaaa aacgggttag tgaatatttt cctggaacga ttaggcgcac
    20041 ctgcgctttc ctggtttagc gatcaaaaac tggtcatgat ccctcttgtt ttgatgtctg
    20101 cttggcaatt cggttcggca atgatcatct ttgctgcagg tatcaaagag atcccaggct
    20161 cgtattatga agcggcagag atcgatggag cgaacaaatg gcagacgttt tacaagatca
    20221 cgttacctct tctttcgccg atcattttgt ataacttggt tatgcagaca atcgcggcgt
    20281 ttatgacttt tacgcaggca tttatcatta cccaaggtgg tccgaatgat gccacgaact
    20341 tttatgctct ttatgtgtat aaccaagctt tttcttttaa ttctatgggg tatgctagtg
    20401 ctatttcgtg gatcatgctg ttgattatgg gagctgtcac ctttctgatt ttcaaatcat
    20461 caaaaaaatg ggtcgttgtg tgaatgagag gaggaagaaa tgaataatac agtaacgaaa
    20521 tggtttcaat acggttttat tttgctatta ggtctagtca tgatttatcc ggtcttatgg
    20581 atgatcgcag gttcttttaa aacggatcaa gagattatga gtggttcgct gaatttgatt
    20641 ccagagactt ttcaatggga aaattacgca aaaggatggg cggggtttgc tggaatttca
    20701 ttttttactt tctttaggaa ttcgtttgtc atctctgcag tttcgacgat agggacagtt
    20761 ctgagctcga cttgtattgc ttacgcatta tcgagaatcg atttcaaagg aaaaagattc
    20821 tggttcgtcg tcatgttagt aacgatgatg attcctgcac aagtgatttt gattccgcag
    20881 tttattattt acaatcggct gaatttggtt gggacgtatg tgccgctgat tttaccacat
    20941 ttttttggac aagcattttt catttaccag atcatgcaat tcatggtcag catccccaaa
    21001 gagttagatg aatcagcgat tattgacgga tgcagcaagt atagtgtctt taccaaaatt
    21061 atttttcctt tgctgaaacc ttcgatcatc acgacgatca tcatccagtt ttattggaaa
    21121 tgggacgact tcatgggacc attgatttat ttaaacaagc cgagatctta cacggtatcg
    21181 attgccatca aattatttgc agacgctgga tctagtacga cttacgcgag tatgtttgcg
    21241 atgtctacgt tgtcgctgct accggttttt ctgattttct tattcttcaa taaatatctt
    21301 gtccagggga tcagtacaag cggtctgaag ggctgaatat gaaaaagcca atagaagaga
    21361 tgtttcttta caagctcaac tactatgtct gtcggatttt tttaaccagt cttatcagta
    21421 atgctctttt cgctttagtt ctaggatgtt tgttcacttt aggattagtg ctgtctgctc
    21481 gtgttgtcta tctgatttat tttttgatga tgtatccttc ttattatcta tctgtccatt
    21541 acttggcaga aaaaaaggta tgttggagga aaggaaaaat caaacggatg tgtttcatca
    21601 ttacgttgtt tcagctgttt gtcattattt ttgcagttga ttggtattta ttagtgggaa
    21661 agattggagc ttggtatctg attccttttc tattgtgtct gttgcttttt gtatcccata
    21721 gtttttttag tacgctaatt gatatctgca aagaagataa agcagatagt cttgtttatt
    21781 tgatcaaaag gaatatctgg ctgttgtcaa aaaacgtcaa ggggtttctg tggtatttat
    21841 gccttttgta tggactgttc tggtgtatac agcatgattt tttactcact gcattactgt
    21901 atccagggct gccatttgtc acagtatttt gcttaaaaga aaaggagaat gactttaaat
    21961 gaagaaaaaa tatgtacaag tcggacttgg tgggagagcg cgtttttttt atgaggcaat
    22021 tgcctctacg tatcatcaaa cttgtgaact agtcggtttt tgtgatgtat ctcaaacaag
    22081 gatggattat gcaaatcgga tacttgtgaa caagtatgga cagaaaaaag taaagacatg
    22141 tatgccatca caatttgagc aactactgga tgacgtacgg ccggctgaag tcattattac
    22201 gtcagtagat cgaacgcatc atgattatat cattcgtgca atgaaaaaag gatatgatgt
    22261 tatttgcgaa aaacctctga ctatcgatga ggaaaaagct caagaaataa taacggtcca
    22321 aaaacagacg ggaagaagcc tgcgagtcac ttttaattat cgttatgcac cgtatcacac
    22381 gaagataaag gaactgatag aaagtgatgt gattggtgat gtgttttcta tccacttcga
    22441 atggctgtta aatacgcaac acggggcaga ttatttcaga agatggcatc gaaataagca
    22501 caatagcggc gggcttcttg ttcataaatc tacccatcat tttgatctag tgaatttttg
    22561 gttgaacagc cgtccccaaa aggtatttgc attcggtgga ttaaactttt atgggatgca
    22621 taatgcggag aaacgcggag tagaaatatt ttacagtcga tgctatggaa gcgaactagc
    22681 taaagctgat ccatttgcta tagacctgtc agccaatgag gaattgcgag aaatgtattt
    22741 agaagcagaa aaagacagtg gatatattcg tgaccgcagt gtattttccg atgatatctc
    22801 tattgaagat acactaggcg taacggtcaa atatgaaact ggtgcgattt tgacgtattc
    22861 gctaaacgcg tatatgccgt gggaaggatt caatgtttct ttcaatggca gcaaaggacg
    22921 gatcgaaatg aaagtgatcg aaaagtctta catcaatgca ggtggagata aaaaggatga
    22981 aggagcagcc gttcttcagt ccatccatgt ctatccgatg ttcggtgagc catatgaggt
    23041 aatcgtcgaa caagaagcgg gcggacatgg cggaggagat ccgcgtttat tagatgatct
    23101 atttggtgaa gaaaaagaag aagatccgtg gaatcgggca gcaactcacg tagatggtat
    23161 tttgtcgatt ttgacaggaa tcgcagcaaa tcattctatt gcttcaggaa aagcagtagt
    23221 gattgacgag cttgtcagct taaaagaata agaatagatt tacggaaatt acttcccgta
    23281 gtttataaaa tcttgcctta ttgccgatag gctgtctttt gaacactatt tattcggaaa
    23341 aaggggctat gacatgcttt ttgtcatgcc ttctttttat gtgggctgct tttttagata
    23401 ggagaaaata aaaataacta tttaaacccg aacaaaaagg aataatgcat taaaaatcat
    23461 tagattttac aaaaaaataa gtaaaccacg ttgtcagttt cagaaagtag tgttagactg
    23521 ttttcaatat ttgaaacaac attttttatt caaatatggc aaactgcctt cacgtgcaaa
    23581 acagttgcaa gaaaatgaat ttacactatt attagtttta catacctact tatatatcgt
    23641 cataatatgg atgacagttt ctagccagta ccgtaaatgc tggactataa gtaaaagatt
    23701 ggctatttca cccttctttt cttatgataa agtggagggt gtttttcttt aacagaaaca
    23761 tagtaggttt ttgcacaaaa gcggttattt cggtattaat aaatgataaa ggagcaaata
    23821 aagtgaaaga attagttgaa cgtattcaaa aagacggacg tatattaggc gagggcgtat
    23881 taaaagtcga cagcttcgtc actcaccaag tagaccctgt attgatggaa caaatgggaa
    23941 aacggtttgc agaagtattt aaagaacaag gaatcacgaa agttgtgacc attgaagctt
    24001 ctgggatcgc accagcttta tttgctgcaa gagaattaga tgttccaatg atcttcgcta
    24061 gaaaagcaaa aagtttgacg atggatgaag aattattgac agcttctgtt tattctttta
    24121 cgaaacaagt gacaagtacg atttctattt ctcgtaaatt tttatcttca gaagacaaag
    24181 tattgattat cgacgatttc ttggcaaacg gacaagcggc aaaaggattg atcgaattat
    24241 gccagcaagc tggagcgcaa gtagaaggaa tcgggatcgt catcgaaaag tctttccaag
    24301 atggacgtca gttacttgaa gaaatgggat tgagagtcgt ttctcttgcg cgtattgctt
    24361 ccttgacgaa tggacaagtt gagtttcttg aggaggacgc ataatcgtgg aaaaagaaac
    24421 acaaaatgga aaagctgcgg ttttagggct gcagcatctg ctagcgatgt atgcaggagc
    24481 agtagcggtg cctcttttga tcggtacagg actaggcttt aatcaagaac agatgactta
    24541 tttgatttca attgatatct ttatgtgtgg attagcgaca ttattgcaat tgacagttaa
    24601 ccgatttttc ggaattggct tgcctgtcgt tttaggatgt gcgatccaag cagtagctcc
    24661 actgatcttg atcggaacca atgaaggagt aggagctatc tacggatcta ttatcgcttc
    24721 tgggatcttt gttgtgttag tttctggtgt cttttctaaa attaaaaaac tattccctcc
    24781 tatagtaaca ggaacggtta tcaccgttat tggtttgact ttgattcctg tagcaatcga
    24841 gaaaatgggt ggaggtaatg caacagcagc tgattttggt gacaaaacaa atctgctact
    24901 tgcatttgtg acgattctcc taattgtagg cgtgcaaatg ctggcaaaag ggttcattcg
    24961 ttcaatcgcg gtattgattg gtttagtcgg aggaagcatc ttagctgctt tcatgggaat
    25021 gattgatctg agcgcgattg gagatgcgcc gttgttccat gttccacaac ccttttattt
    25081 tggtaaacca actttcgacg tatggtcgat tttactaatg atcatcattt ccatggtcag
    25141 catggtagaa tcaacaggtg tatattttgc acttggtgac attactggga aaaatgttgg
    25201 agaagaagaa ttgaaaaagg gatatcgtgc agaaggactt gccgttatct taggcggtat
    25261 tttcaataca ttcccttata ctggcttttc tcaaaatgtc ggattggttc aactttcggg
    25321 aatcaaaaca agaaaaccca tctatttctc tgcattcttc ttgattgttt taggattatt
    25381 ccctaaaatc ggtgcgttag cacaaatcat ccctgagccg gtacttggtg gaggaatgtt
    25441 agtcatgttc gggatggtgg cagtccaagg aatccgtatg ctgttggaag ttgatttcag
    25501 caatgataaa aatctattga ttgttgctgt ttctgttgga tttgggctgg gtttcaatgt
    25561 tacgccgaca ttattcagtc aaatgccgca aacattgcaa atgttcactg gaaatggcat
    25621 cgtaatgagt agcattacgg ctatcatatt gaatttgatc tttaatggat tgaaaaaaga
    25681 tgaagaaata ttggaagaag ccttcaagaa gtaatagagc aaaagaaagg ttgtgaaatc
    25741 agcgttttga cctgattgtt agagaaacaa acggaaaaat agttcctcgt attttgccgt
    25801 ttgtttgagc taataccgag ggtcagctga ttgaacaccg ttgaaggaag aggtcgtgag
    25861 cctgccgttc atctccgagt cacaaggaac aatttcaaaa aaaagcttct catgtttgtt
    25921 gaatattgtt ccttgtgacc gaggagttgg caggagaaca ccgtttatcc ataaaaagga
    25981 atgtgaccta acttttgtca cattcctttt ttttatcttt aataaacacg aacaataaaa
    26041 tgtttttctt tgttaacatt cgaaatttct ttgacatcaa aaaaagtgat tgttaaaata
    26101 tgaatgtaga aaaactggga ggaaaaacaa tgacagcaaa aatctctgtg attatgggga
    26161 gtacttcaga ttgggaaaca atgaaagaag catgtcaaat attggatgaa ttcggtgtgt
    26221 cttatgaaaa aaaggtcgtt tctgctcatc gaaccccaga tttgatgttt gaatatgcac
    26281 aaaacgctcg aaaaaaagga atcaaaatca ttattgcagg agcaggggga gccgcgcatc
    26341 tgccgggtat ggtcgcatca aaaacaactt tgccggttat cggtgtaccg gtccaatcac
    26401 gaacattaaa tggtttggat tcgcttcttt cgattgtcca aatgcctgga ggagtacctg
    26461 tagctactac agcaatcgga aaagcgggag caattaatgc tggactactt gcgatacaga
    26521 tgctttccat gtatgattta gaattggagg ctaaattggc tgaaagacgt tcaatgttgg
    26581 ctaaaactgt tatagaaagt agtgatcaac ttggttaaac ctttgttgcc tggttcaacg
    26641 atcgggatcg tcggtggcgg tcaattaggt cgtatgatgg cgattagtgc aaaagaaatg
    26701 ggctttaaag taggtgtgct ggatcctgtg agcgattgtc cagccgcaca agttgcagat
    26761 tggcatattg aaagtgtcta tgatgacacc tttgcgctga aagagcttgc cagaagaaca
    26821 gatgtcatta cctatgaatt cgaaaacgtc agtgtagagg cgttaaatgc gattttaccc
    26881 atgtcattta ttcctcaagg gacagacttg cttgcgatta cgcaagaccg cctgttagaa
    26941 aaatcttttt tggaaacgaa taacatcgtg attgctcctt atgcaacgat cgtcagtccg
    27001 acggatatcc aagatgcgat tgacgggatc ggctatcctt gtgttttgaa aaccactcga
    27061 ggcggctatg atggcaaggg gcagtatgtt ttgaaaagtc gggcggatct agctcctgcc
    27121 atggaccttt taagagaagg aacctgtgtt ttagaagcat ggatcccgtt tgaaaaagag
    27181 atttcgatca tggtggcagg aaatggtcaa ggagatttca cgacttttcc ggtcgtggaa
    27241 aacatccacc acaacaatat tcttcatgaa acgattgctc cagccgcgat tgatcaggat
    27301 gtgatcgagg aggctgagcg tattgcccgt gtgattgcag aagcagtgag cctttcaggt
    27361 gttttagggg tagaaatgtt tttaacaaaa acaggcgggc tatatgtgaa tgaattagct
    27421 cctcgtcctc acaattctgg acattattct attgaagctt gttctatgag tcagttcgat
    27481 gcccatattc gaggtatttg tggttggcca ttaggagaaa aagtacgttt gataagcaat
    27541 gcagtaatgg tcaatgtttt gggagaacaa ctgctggaaa gctatcgctt gattgagaat
    27601 aagccagact ggcaattcca tttctatgga aaaaaacaag caaaaaaagg tcgcaagatg
    27661 ggacatatca ctatcttgac agaagacatc cagcaaacgc tggaagaaat caaacaaacg
    27721 aatatttggg attagaggag aaaaaacatg ttagaacgtt atacacgccc tgaaatgggg
    27781 aatatctgga cggaagagaa caaataccgt gcgtggctcg aagtcgagat tttagcagat
    27841 gaagcttggg cagaacttgg tgaaatcccg caagaagatg tgaaaaaaat tcgtgagcat
    27901 gcatcatttg atatcgatcg gatcttggaa atcgaaaaat taacacgcca tgatgtcgtt
    27961 gcgtttacac gagcggtttc tgaaacattg ggagaagaaa gaaaatgggt ccattatggt
    28021 ctaacaagta cggatgtcgt agatactgct tatggctatc tcatcaagca agcaaatgat
    28081 attttacggg aagacttgaa acgttttacg gaaatcattg gcgaaaaagc aaaagaacac
    28141 aaatatacag tgatgatggg tcggacacac ggtgtccatg ctgaaccaac tacttttgga
    28201 ttgaaacttg ccacatggta ttcagaaatg aaacggaaca tcgaacgatt tgaacatgcg
    28261 gcaaaaggag tagaagcagg aaaaatctca ggagcagtgg gaacgttcgc aaatatcccg
    28321 ccatttattg aagagtatgt atgtaaacaa ttggggatcc gtccacaaga aatttctact
    28381 caagttctcc ctcgggatct acatgctgaa tatttctcag cgatggcgct tattgctaca
    28441 agtattgaac gttttgcaac agagatacgc ggattgcaaa aatccgaaac acgtgaagta
    28501 gaagaatttt ttgcgaaagg tcaaaaaggt tcttctgcta tgccgcacaa aaggaatccg
    28561 atcggttcag aaaatatggc tggtctagcc agagtggtgc gcggacacat ggtcactgcc
    28621 tatgaaaatg tcacattatg gcatgaaaga gatatttctc attcttcagc agaacgaatc
    28681 atcttgcctg ataccacgat tttattgaat tacatgttaa atcgttttgg caatatcgtt
    28741 aaaaatctaa cggtctttcc tgaaaacatg aagcggaata tgtctgcaac atttggtttg
    28801 atttatagcc agcgcgttat gctgaaattg atcgataagg gaatgacaag agaagaagct
    28861 tatgatcttg ttcagccaaa aactgcgtac gcttgggaca accaagtgca gttccgcccg
    28921 ctgttagaag aagatccaac catcacatcg gtattatcta aagaagaaat agacgatgcc
    28981 tttgattatc atcatcattt gaaaaacgtt gacgtgatat tcaaacgagt aggtttggaa
    29041 taaagaagag tgagttgcaa aattcaatta aaatagaata gcaaaaataa ccaaaaaaga
    29101 agcagatccg ttagcggttc tgcttctttt agtttattat attttcttat caataatagg
    29161 aacctgattt taaaagcggc tacctcttta ccgtgcctct ttacatacaa aaaatagagg
    29221 aattaagcgt aaatggcttg gctattataa ctaaaatgct ggttatggag atgttgcagc
    29281 aagttttcga gaaaagcgta ttccatctct tgtccattga ataagatctg gatacgataa
    29341 tgatttttga aacgcttctg ttcatgaatc tgactgattg aaagttcgcc tttgtgttct
    29401 gtctgataaa gaaaagtcct tttacggcat tcagcacagc gcttggttgc tcggcggatt
    29461 tttcggaatg tataaaaact agtatcttta ttgcataaat aaacgtcttt gatcgacagc
    29521 aaatcattat atagacagga acgttcttca gctgttaatt ctgataaaaa aagctgacct
    29581 tgttttattg catcttccat cggcaagtgc ctcctcgaac ccattatcca aatttttagt
    29641 taattctaat ataccatcat taagtataaa tgtaaacgct tgttgatggt ataagatttt
    29701 cgtttctgat aaaacacgaa cattaaaaga atctaatgcc ttaaatgtac atatttttgt
    29761 tgatttataa aaagatgatt gctaaaattt aaagtgaaaa aacgaacaaa aaacgatgta
    29821 tttgctttta atgtaaataa aaaggagaga cgtacatgga gaagaaagaa cttttgtatg
    29881 aaggaaaagc caaaaaaata tatcttactt ctgatgagaa cttgatttgg atagaatatt
    29941 tagatcaagc aactgcgttg aatggcgcga ggaaagacca gataagtgga aaaggagaat
    30001 tgaataatca aatcacaagt gcaattttcc gttatctgac tcaaagagga ataaaaaatc
    30061 attttattga acaattgtct aaaaccgaac aattagcaaa aaaagtacaa attatccctc
    30121 tggaagtggt tatccgaaat attgcagcag gaagtttctc aaaacgtcta ggtgttcccg
    30181 aaggtaaaga attcatcaaa ccaatcattg aattctacta taaggatgac cagttggatg
    30241 atccatttat caatgaagag catatctatt tcttacaact tgcaaagccg gaagaaatca
    30301 gtgagctgaa agcccaagcg ttggctatta atgaaatttt gaccgaatta tttgctaagg
    30361 tcggtttggt tttagtagat ttcaagctag aatttggccg gtcgaatgga caaattttgc
    30421 ttgcggatga agtaacaccg gatacttgcc gattatggga tgaacgtacg aatgaacata
    30481 tggacaaaga cgtttaccgc agagatctag gtgatttgat cccagtctat caagaagtat
    30541 ataaccgtct atcggaacta actgaagaaa aggagcgagt atgatgtatt tcgtaaaagt
    30601 atatgtaacc tataaagatt cagtgttaga tcctcaaggg gaggcagtaa aaaaagcagt
    30661 ccatcgtatg ggatatgaaa caatcgaaga tatccgaata ggaaaatact ttgagataca
    30721 acttgctgat gcagaagaac caatcgatac agtgattgaa acgatttgtg acaagctgct
    30781 tgcaaatgta aatatggaaa cataccgata tgaaatcaaa aaaatggagg aagtataaca
    30841 tgaaatttgc ggtcatcgtt tttccaggtt caaattgtga cgcagatatg ttatgggcag
    30901 ttcgcgagat catgggcgct gatgccgaat ttgtacggca tgatgagtcc tctttagaag
    30961 gatttgatgg ggttttactg ccgggaggat tctcctatgg tgactatttg agatgcggtg
    31021 cgattgctcg gttttctaca atcatggaag aagtgattcg ttttgctgaa gaagggaaac
    31081 cagtattcgg tacatgcaat ggattccaga tattgacaga agcaggactt ttgccaggtg
    31141 tactgagacg aaatgagtct ttgcatttta tttgcaaaac tgtaccattg aaagtcaacc
    31201 ccaagacggc ctttacttca gagtatgaag ccgacgaaat aatctatctt ccagttgctc
    31261 atggggaagg aaattatttc tgtgacgaag aaacacttca agaactaaaa aaaaataatc
    31321 agattgcctt tacttataat gacaacatca acggcagcgt ggaaaacatt gcagggatta
    31381 cgaacaaagc agggaatgtg ttaggaatga tgcctcatcc agaacgtgca gtagaagact
    31441 tgctaggatc agaagatggg aaacggttct ttgcttccat tttaaaaaac ttcggaaagg
    31501 tgaccgtata gatgaaaaca ttgaaagaac cgacagctga agagatcaag aaaaaaagaa
    31561 tctatgccga atggggattg actgatgaag aatatcgaat gatcgaagaa gatatcttag
    31621 gaagaatgcc taattatacg gaaactggac tgttttctgt tatgtggagc gaacattgtt
    31681 cctataaaaa ttctaaaccg gttctacgca aatttcctac tagtggacct catgtgttgc
    31741 aaggccctgg agaaggcgca ggcatcgtcg acatcggtga tggactagca gttgtcttca
    31801 aagcagaaag tcacaatcat ccttcagccg tggagcctta tgaaggagct gcgacaggag
    31861 ttggcggaat catcagagat attttcagca tgggggctcg tccaattgcg ttgctagatt
    31921 cattgaggtt cggcgaatta gataatccaa gaacgaaata tttactagaa gaagtagttg
    31981 caggtatcgg cggatacggc aattgtatcg gcataccgac tgtaggcggc gaaattgcat
    32041 ttgatccttg ttatgaagga aacccattag tcaatgcaat gtgcgtaggt ttgattgaac
    32101 acaaacatat ccagaaaggg caagccgctg gcgtcggtaa ttcgatcatg tatgtaggtg
    32161 caaaaacagg tcgcgacggt atccacggag caacttttgc ctcagaagaa tttaatcaag
    32221 aagaagaaca gcaacgttct gctgtacaag taggcgatcc ttttatggaa aagctgctgc
    32281 ttgaagcgtg tttggaatta atcttgcatc attcggattc tctaataggt atacaggata
    32341 tgggagcagc aggacttgtt tcatctagtg cggaaatggc ttcaaaagct ggctcaggac
    32401 ttatattgac attagatgaa gtcccacaaa gagaaactgg gatgacacct tatgaaatga
    32461 tgttatcgga atcacaagaa cggatgttga tttgtgttaa atcaggagag gaagaaaaaa
    32521 tccaagaact atttcagaag tatgatctag atgcagtcac tatcggtaaa gtaacagacg
    32581 acgggcaata tcggctatat catcaaggag aagaagtagc aaatctgcca gtcgatgcat
    32641 tagctgaaga tgcaccagtt tatcataaag aaatgaaaga accagctcga atcgtagaat
    32701 ttcagcagtt agctccttat cagccagtta tcgaagaacc tggacaagta ttgcttgatc
    32761 tgctgcagca accgacgatt gcttcaaaac gttcaatcta cgaaacttat gattcacaag
    32821 tacaaaccaa tacagttgtc cgtccaggaa gcgatgctgc tgtgatgagg gttagaggga
    32881 cgaataaagc gttagctatg accacagact gcaacgcgcg ctatctgtat ttggatccaa
    32941 aaatcggagg acaaatagca gtggcagaag ctgctagaaa tattattgct agcggtggta
    33001 agcccttggc gatcaccgac tgcctgaact atggttctcc tgataaacca gaagtttttt
    33061 gggaattatc gacttcagct gacggaattg ctgctgcgtg tgaaacgtta ggaactcctg
    33121 ttatttcagg aaatgtctct ttatataatg aaacggatgg tcaagcaatc tatccaacac
    33181 caatgattgg tatggtcgga ttgattgagg atcataaaca tatcactact caggaattca
    33241 aaaaatcagg ggatctaatc tatatattag gtaagacgtt tgcagatttt gatggcagtg
    33301 aattacaaaa gatgcagctt ggaagaatcg aaggtgtgat ccgaaacttt gatctatcca
    33361 ttgaaaagag aaaccaagag ttagtattga cagccatcca aaacggattg attgaaagtg
    33421 ctcatgactg ttcagaaggt ggcttggcaa ttgcactggc agaatcagct ttcaaacatc
    33481 agctagggat atctgtacag tttgaattgt cctctgctca attatttgca gaaactcaat
    33541 caagatttgt gttgacagtc gcaccggaaa ataagacacg attcgaagaa atgatgggag
    33601 atgctgctgt tttagcaggt aaggtgacgg atgaagcgat aatcgaaatt tcagcaactg
    33661 acggtcagat caaaatagaa actgcagtag caagaaagtg ctgggaggat gcaatcgtat
    33721 gtcttatgaa gtgaagagtt taaatgaaga atgcgggatc ttcggtgttt ggggtcatcc
    33781 agacgccgct agtgtggcat acttcgggct ccatagtctt cagcatcgag gacaagaagg
    33841 tgcaggtatc gtgtccaatg atcgaggaaa gcttaagggg catcgagatt tggggctgct
    33901 ttctgaggta ttcaaagatc aaagaaaatt gcagcagtta acaggagaag ctgcaatcgg
    33961 acatgtacgt tatgcaacag caggcaatgg aagtgtggac aatattcagc cttttctatt
    34021 caaatttcat gatcagtcga tcggcttagc gcataatggt aacttgatca atgctcgttc
    34081 ccttagaaaa gagttagagg aaaatggggc gatcttccat tctaactccg atacggagat
    34141 attgatgcat ttgatccgac gaagtgaaga aacagatttt ttaaataaat taaaaagtgc
    34201 tttgaatgaa gtcaaaggtg gctttgccta tttattaatg acagaaacgg caatgattgc
    34261 cgctttggac ccaaatggtt ttcgtccatt agcaatcgga caaatgaaaa acggagcata
    34321 cgtgattgcc tcggaaactt gtgcattaga agttattggg gcacgtttta taagagatgt
    34381 ccagcctggt gagatagtga tcatcaatga cgaaggaatc cagattgatt gctttacgaa
    34441 ggacacgcag ctttctattt gttcgatgga atatatctat tttgccagac cagattcaaa
    34501 catcgcaggg atcaatgtgc atactgcacg gaaaaatatg ggacggaatt tagcgaaaga
    34561 atcgccagta aaagcagata tggtcgttgg cgtacctaat tcttctttgt cagccgcaag
    34621 cggttatgcg gaagctagtg gaatccctta tgaaatcgga ttagtaaaaa atcaatacat
    34681 tgcccgaaca tttatccagc caacgcctga gctaagagag caaggtgttc gaatgaaatt
    34741 atctgctgta cgaggagtag tggaaggtaa aaaggtgatt atggtcgatg attcaattgt
    34801 tcggggtacg acaagcagac ggatcgtcca tcttttgaaa gaagcagggg caaaagaagt
    34861 ccatgtgagg attgcttctc ctcctttgaa atatccatgt ttttacggga ttgatattca
    34921 aacgcgtaaa gaactcattg cggcaaatca cacgattgat gaaatcaaag aatgtatcgg
    34981 tgcagattct ttaggttttt taagtgaaga gggtctgatc gaatcgatcg gtctggaaaa
    35041 agatgctccg tattcaggat tgtgtatggc ttatttcaac ggagattatc cgacgccgtt
    35101 atatgactat gaagaaaaat atcaggaatc actaaaagaa aaagtatcgt tcttttaaaa
    35161 gcagaccaag aagggagtat atgaattcaa tggcaaatgc atacgcaaaa gctggcgtag
    35221 atgtcgaagc tggatatgaa gtagtagaac ggatcaagaa gcatgtcaaa aaaacggaac
    35281 gtttaggtat gatgggcgct ataggaggat tcggcggttg ttttgattta tcggtctttc
    35341 aattaaaaga gccagtcctt gtctcgggga ccgatggcgt ggggacgaaa ttagcagtgg
    35401 caatcgaagc agacaaacac gatacaatcg ggatcgactg cgtagcgatg tgtgtcaatg
    35461 atatcttagc acaaggcgca gagccgttat atttcttaga ctacttagca acggggaaaa
    35521 atgatccgtt acgtttggaa aatgtagtaa aaggtgtagc agatggatgt gttcaaggaa
    35581 atatggcgct gattggcgga gaaacagctg aaatgcctgg tatgtactca ggtaatgatt
    35641 atgatctagc cggatttgct gtagggatag ctgaaaaatc tcaattattg acaggagaaa
    35701 cgatcaaccc aggagatgtg ttgcttggaa tagcatctag cgggattcat tcaaatgggt
    35761 actctcttgt tcgcaaaatt ttctttgagc agcagcagtt ttctttatca tcaaaactgc
    35821 cagaattgaa aaaaacactt ggcgaagaat tgcttgagcc aacgaaaatc tatgtttctt
    35881 ccttgcttcc gctgatcaaa gaaaagaaaa taaaaggagc tgcacatatc actggtggag
    35941 ggtttgtaga aaatatcccg cgaatgttac cagaatctat ggctgcacaa atcaaacttg
    36001 gctcatggcc gattcttcca atattcggct gtttacaaaa atatggtgaa attcctgaga
    36061 tggagatgta tgaaatcttc aatatgggca tcggtatggt cctggcagtc gaagaatcga
    36121 aagtaaaaga aatagtacac gaaattgagc aagctggcga aaaagtttat gaaatcggga
    36181 aagtgatcgc caaagaaaaa catcgtgtgg tgtttatagg agaagaacga tgagaatcgc
    36241 tgtttttgct tctggaaacg gaagcaactt tcaagcgcta gccgattatc tatcgaaaaa
    36301 aggattggaa tcttcgattg actggttgtt ttgtgatcag cctgaagcat atgttttgaa
    36361 acgtgcaact gcgttgagtg taccggcaga ttgtttttca cctaaagaat ttgattcaaa
    36421 aaaagagtat gaagaagcaa ttttgcataa attgaaagaa aaaaagatcg atttgatcgt
    36481 attagctgga tatatgcgaa tcattggacc ggtattgctg aaaaattatg ataaaagaat
    36541 catcaatatc catccgtctc ttttacctgc cttccctggt ttgcatggca tacgtgatgc
    36601 ctttgaagct ggagtaaaag aaacgggtgt cacaattcat tatatcgacc aaggtgtaga
    36661 tactggacca atcatccggc aggaaaaagt gagaattgaa caggaggaca cgttcgattc
    36721 tttagaagaa aaaatccata gagtagagca tcgaatctat ccagaagtga ttagtgaaat
    36781 tattgaaaat ggaggctatc tgaaatgaca agagcattga tcagtgtatc agataaaact
    36841 gggattgtag cattcgccca aggtttgatt gaacaaggag tagaaatcat ttcaacagga
    36901 ggaacaaagc atgcgttgga taaagctggg atcaaaacgc tttcgatcga agaagtgaca
    36961 gggtttcctg aaatgatgga tggccgtgtc aaaacacttc atcctaaaat ccatggtggt
    37021 cttttaggaa gacgcgatct ccctagccat atggaggcaa tggaaacgga aaatatccaa
    37081 ccaatcgact ttgtttgtgt caatttatat cctttcaaag aaacgattat gaaaccagat
    37141 gtcgaaatag cagaagctat tgaaaacatt gatatcggtg gtcctagtat gctccgtagt
    37201 gcagcaaaaa accatgaatt tgtcacctct gttgttgatc catctgacta tgaagaagta
    37261 ctttcagaat tacgtcagta tgggcatact tcactttctt tcagacaaaa actagcggca
    37321 aaagtctttc gtcatactgc ggcatatgat gcactgatcg ctcaatactt aacggattgg
    37381 gtggaagaaa aagaaccgga aaaattgaca cttacgtatg aacgaaaaca aacgcttcgt
    37441 tacggtgaaa atagccatca agccgctgcc ttttatcagt ccgctcttct ggaaaaatat
    37501 tcgatcgcag cagcaaaaca actccatggg aaagaacttt cttataataa tatccgagat
    37561 gcagatgctg cgctacggat catgaaggaa tatgacgagc caacggtagt cgccttgaaa
    37621 cacatgaatc cttgtggtat tgggacagca tctacgattt tagaagcgtg ggaagcagcg
    37681 tatgaagcag atcctgtttc aatctttggt gggatcatcg ttttgaatcg agaagtagat
    37741 ctgccaacag cagaacagat gcataagctg tttctagaaa tcatcattgc cccttcttat
    37801 acacaagaag ctttggaatt actgcaaaaa aagaaaaaca tccgattact gacagtagac
    37861 tttgcgcagg aagaaaatca aaatcgagaa gaaacggtct cagtcttagg tggacttctt
    37921 gtacaagatc aagatctggc aatagaaaat gaagaagagt ggaaagtcgt gacaaaacgt
    37981 aagccgactc cagcagaagc aaaagcatta gtgtttgctt ggagagctgt caaacatgtg
    38041 aaatctaatg cgatcgtact ggcaaacgaa cgtcagacag taggaatcgg agccgggcag
    38101 atgaatcgag tcggttcagt aaaaattgcg attgaacaag cgcaagagaa aatggaaggt
    38161 gcagttttag ctagcgatgc ttattttcca atggatgatt cggttgaata tgcagccaaa
    38221 catggtataa aagcaatcat tcagccagga ggaagcatca aagaccaaga ttctattgat
    38281 atggcgaata aatacgatct agcaatggtc tttactgatg ttcggcattt tagacattag
    38341 agaggagtcg gataaatgaa cattttggtg atcggtgcag gtggtcgtga acatgcaata
    38401 agtaaaaaac ttttagccag tccgaaggtg gatacggtgt attgtgcgcc aggaaatccg
    38461 gggatgacaa aagatggtat caattgtgtc gctatcagtg agcgagatca ttcagcgtta
    38521 atccattttg ttaagaaaga ggctattgat tggagtatcg ttggtcctga agttcctttg
    38581 ttaaatggga tcgtcgatga ttttatggcg gaaggattga aaatcttcgg tccgacaaaa
    38641 gcagcagctc tgatcgaagg ttcgaaaagt tttgctaaac aattaatgga tacttatcaa
    38701 atcccgacag ctaaatccag aagttttgaa tgttacgaaa aagcagcagc gtatatcaaa
    38761 gaaaatggtg cgccaatcgt agtgaaagcg gacggcttag cagctgggaa aggtgtagtc
    38821 gtggctacca cagtgaagga agcgctggat gcggcagaac aaatgttgaa ccagcatcga
    38881 tttggggata gtagcaaaaa aatcgtcgta gaagagtttt tggcaggaga agaattctcc
    38941 cttttggcgt ttgtccgtaa taccaaggtg tatccgatgg tcatttcgca agatcataag
    39001 cgggcatttg ataacgataa agggccaaat acaggcggaa tgggtgctta ttcaccagta
    39061 ccgcaaattt caaaaaagat gattgaaaaa gctgtggaag aagttttaaa accagcggct
    39121 ttgggaatga aagaactagg acgctctttc acgggtgttt tatatgcggg tttgattgct
    39181 acgaaggaag gaccaaaagt gatcgaattc aatgcgcgtt tcggtgatcc tgaaacgcaa
    39241 gtcgttcttt ctcggttgaa gagtgacttt gcgcaagtaa tcgatgattt attagagaat
    39301 cgtacagtag agttaaagtg gcagcaagaa ggatacaatg taggcgttgt ggttgcagct
    39361 gccggttacc ccgaagaata tgagacagga atggttctac cagattttct ggaagaagag
    39421 ctttctgttt actatgctgg agtttcctca aataacggca aactagttgg agcaggagga
    39481 cgtctttatt tagtagaagc atacggtgag acgatcaaag aagcacagaa aaaaatttac
    39541 accgcgttaa tggataaaac gacagatgga acattttatc gtacagatat tggagaaaaa
    39601 gctgccaata gcgaactgat cgtgaagtaa atcagctgct tccatttata aaaaatacaa
    39661 caaacataat cggaagcgcc gattttagtg aagccttttc ataaagagtg tggtacactt
    39721 ttgttgtgta tataaataag gaggagttca gatgaaaaaa attcaattag gtacttctga
    39781 tcagaaagca gctgcagtga tcttaggctg tatgcgtatc aacaatgcgg aagatccagt
    39841 aaaagtgatt gagacagctt atgaaaacgg cattgatttt tttgaccatg cagatattta
    39901 tggaaacggt gaatgtgaag aaatctttgc agatgctcta gcaaaaacat caatcaaacg
    39961 agaagatctt tttattcaaa caaaatgtgg aatcgttcca ggtgtgatgt ttgatttttc
    40021 taaaaaacat atcattgaat ctgtagaaaa aagtttgacc cgtctgaaaa tggattatgt
    40081 ggatgcttta cttcttcatc gtccagatac attggtagaa ccagaggaag tagctgaagc
    40141 gtttgatgaa ttggaacggt caggaaaagt tcgttatttt ggcgtcagca atcaaaaacc
    40201 aatgcaaatc gaattgctga aaaaatatgt tcgtcaacca ttagtagcta atcagctgca
    40261 atttggcttg aaacatactg gaatgatcga tcaagggatt catgtcaata tgacagatga
    40321 agcaagtatc gatcatgacg gtagtgtact tgattattcc cgattgcatg atatgacgat
    40381 ccaagcatgg tcaccatacc agtatggatt ttttgaaggt gtatttatcg ggaacgaaaa
    40441 attccctgaa ttaaaccaaa cattagaaga actagcagct aaatatcaaa cgacaccaac
    40501 tggcttggct tctgcttgga ttttacgcca tccagccaat atgcaagtga tcgccggtac
    40561 gatgaacgta gggcgaatca aggaaatcgc tcaagcttca gaaattaata tgtctcggga
    40621 agattggtac gaattgtacc gtgctgctgg aaacatttta ccttaaagat tgtcataagc
    40681 aggtcaaaag ggctataaat taaaaagaca gaataacttg aaagctgttt tcagcttcga
    40741 gatattctgt ctttttttat tgcaaatgga aagagtatca ttgtacggat ttcgtttatt
    40801 agttattttt gtttttaagg aaaagctgtg tccgatattc ttttggagaa atcccttgtt
    40861 ttttcttaaa taaataactg aaatagttag ggtcattata accaatttca aaagcaatat
    40921 ctgaaatacg atagtctgat tgctgcaata atttttttgc taattcgatt ctttgttctg
    40981 tcagataatc gatgaatgtt tggttaaacg attgagaaaa aatcgtactg aaatgagagg
    41041 gacttaatgc aacttgttga gcgacccaag ttaaggacat atctggatcg gtaaaatttt
    41101 ctttgataaa atccaatgca tggttcaaaa ttggctgata tttgttcctt gaaggctgtg
    41161 agggcagagc agttaagaaa gaaatgaaca tataaactgt cgaacggtaa agcgagtcgt
    41221 cggcgctgat tgctaagcgt tgactggtgg atgtgcaatg aaccatttct tgcgggattt
    41281 tcttttcttt caggcagaca tgctggagta gcttagtcaa gacaaataaa cgatataatc
    41341 gtgttcgagt tggtgtgcct tgtggtttcg tcaagtgttc gatgtactta gatacttcgt
    41401 ttttcgataa tgaagacaaa tcaagtgaga ggtcaaagta aaagacagaa tcaactggaa
    41461 gtttcgcttc ttgaatcaaa tcctcatagc tgataatttg ttctttaagg gaactggcgg
    41521 tttcaagtat catctgcgtg atctcaaaag atgcaggtaa ttcgctgatt ctttctgcga
    41581 caggaccaat ggcgacagaa aaattatttt tttgtccctt ttctaattca tggaccaacg
    41641 tttgggctac ctgataactg gtctcgatca cattttcttc aagagaatca actattaaaa
    41701 atttgatgaa tcgagaggaa acagcagaga agatcacttg ttgattctcc ctaaataaaa
    41761 acttcaaata atcactgaga tgagcgtaat cttcaaagtc tgtactgact tggtttgctg
    41821 ctaataatac ggtcattttt ttccctgcta aagagcgatt gaaagattca gcaagtttca
    41881 atgtttcact gatcgtaagt gagcctttga acaaaccgtt caagaaatga ttttttttca
    41941 attcgaaaac aagatcagaa gaagtgtgcg tttctttttg ctgcaaatct aaagcagtta
    42001 ctgctttttc tattgcttgt ttcaattcgc tacttttaat cggtttaagc agataatcat
    42061 ctgcctgtac ctggattgct cctttcatat aatcgaaatc gtcgaatcca ctaataaata
    42121 tgagacgagt ccaaggaagt agtttccttg cttcttttga aaaagtcaac ccgtccatga
    42181 aaggcatttg gatatcagtc agaatgatgt cgggctgtaa gtcgataatt gaagacagtg
    42241 ccatctcacc atctccagct tcgccgacat aagtgattgg ataataagaa ccaaggacag
    42301 ttatttgttc ccgcaagtta ttgcgaatga ggtattcgtc ttcgatgata aaaactttat
    42361 acatgtttgt ttcctccaat tgttttggga gatagaggga tcatcagtgt gacagttgtt
    42421 ccttctcggt atttgctgtc aatcgtgata ggtttttgat catttccgta ataaagaagg
    42481 agacgcttgt ttacgttgta aagtccgtag ccgatactga aatctgaatc gatccctttg
    42541 gctaactctt tacgtatttc tgccagtttt tctgcaggga tgccaatccc gttgtctata
    42601 actttgaatt ggatcatctc atcaattttg catgtggtaa tacgaataaa tcctttgtga
    42661 cggacgtact tcgttccatg gtatacagca ttttcaatca atggctgcaa gatcattttt
    42721 aaaatagaaa caggaaaaag tgtttctggt atatcgatct tatagtcaag catatggccg
    42781 tatcttattt ttaagatgac tagataatca ttgatatggc taatttcttt ttcaagagga
    42841 atccagtctt gtcctttgct taaagacgta cgaaaaaagc cggaaagtgc atgggtcata
    42901 gtcagagctt cttcaaagca gtcttgttcg atcaaagaaa taatggcatc caaactgtta
    42961 taaataaaat ggggagtaat ctgagcttgc aatacacgaa cttcactttg tgctaaatgg
    43021 tattgtttca aagcgttctc ccgtaaagac tggtttagat cttccgccat tttattcatg
    43081 ctagcggtaa gtgtttgtaa ttccagtgtt tccggcaggg ttaaacgaaa gccaaggttc
    43141 cctgaagaaa gttgttctga catctcgatc agctgattga tcggttcttg gacctttctc
    43201 tctaaaaagc gacgattctt tttcgcatag aaaaaaatgg tactaagaac tccaagttcc
    43261 actattgtta atagaatgag cgagcggaca agtgtcgtat tttttttgct tgctaagtcg
    43321 atctccacac gaacaaaatc ttgtaagata tctgacagca gttgagtgac agaatctacc
    43381 tgattgatga tctcttcatt ggtatccata aaatcttttg ccaagatatt attgatcagc
    43441 tcgttttgat aatcttccag tgtatcgatc gtacgtagcg atacatctaa cgtactaatt
    43501 tctttaagtg tatttgtatt atttttgctc tctcgaatat cattcctaag ttcttctagg
    43561 atattttcct cattgaactc atctacggtt acttggccag aaatcaagtc ccacatactt
    43621 tccaaaactt tttgatcaac cttcaaagca attgcatctg ctttttggat atttgcgatc
    43681 gtctgttgat aaatcaaaag ctgttgcgaa tataacagta tgctcagtag caaagggata
    43741 acagcaaata gaagcatggc tttgtttgct tgggcaaaaa tctgttttat gcttaaattt
    43801 tttgtcatga tacttctcca gtcaataatt tctggttggg atgttagcta tattctgctg
    43861 ggaaaagaca gtctcggaaa taaaaatatc tttaggtaat gtttttcctg tttgaatatt
    43921 tccaaaataa cgagtaacgg tattggctac ataccagccg acatcaggtt tacactcgac
    43981 tacacaattg acgattcctg agcgtagctt ctcgatcata tcttcttgtc catcaatagt
    44041 tacaatgatc aaatcttctc caggtttgat tgatgtttct tttatcgcag ctaaagcacc
    44101 gtgagtcatt tcatcactat ggctgtataa tacatcgatt tcggataaat catttgtttc
    44161 taaatatcgt ttcatctggt cttttccttt catccgaata aaatcaccga caattcggtg
    44221 aacaatagtc atcggaggtt tatggtcgat ggttccatct ttttttaggg ttggtttacg
    44281 attgattgat tccaaaaatc cttcggagcg tagcttggtc ggtgtggagt tctctgaacc
    44341 agcaagttct aggattctga tttcctgttt ctttgaattt gtgaagtgat tgctgacaaa
    44401 taaacccgcc cgatttcctt ctgctttgaa actggggcca atgtgggtga cgaaaagctc
    44461 gggatcattc gtttgaatat gtctgtcgac aagtatgaca gggatccctg ctcgctttgc
    44521 ttcttttaat acagagtccc acccggattc ttgcaaagga gtaaaaatga tcatatccac
    44581 tttataggca atgaacgtcc gtatatcttg gatttgccgt tcctgattca tgtagccatt
    44641 tcggtagaga acttggtaat cttctttttc aagcgcagtg ataattgatt cagtatgctt
    44701 tttccgccag ctactctccg ttcctgattg cgaaaaacca acgactagtg ggtcaggtgc
    44761 ttcctttgta ttaggtgtac accccatcat ttggtaagcg attacaacaa gggctaaaaa
    44821 aaataaaaca cgtttcactt taaatcactc catctggatt tgttagtact atcttatcag
    44881 ataaaaggga aaatcaaagt caaaaggaat caatcgaaaa aaatccaaga aacttaaaaa
    44941 aatccaagac aaaaaaagta cacctaaaaa atttaaagta aatcccataa aaaaatcaat
    45001 gttaacgctt tctttattag ctatactcgg tgtatcaatt gaaatggggg ataaaaaatg
    45061 aaaaacaaat ttagaaagat tgttgggact atcgctattt tgaccgcaag cattctatta
    45121 agtgcgtgtg gcaattcagg atctgcagat gatagtacgg ggtatgtagg aatcgctatg
    45181 ccgaccaaat cagcagaaag atggatcgct gatgggaaca acatggtctc ggaattagaa
    45241 aaactgggtt ataaaacaga tcttcaatat ggagaagata aagtagaaaa tcaagtagca
    45301 caaatagaaa atatgattac aaaaggggta gatacacttg tgattgcttc tatcgatggt
    45361 tcggctttaa ctgatgtgtt agctaaagca aaagaagcag atatcaaggt gatcgcctat
    45421 gatcgtttac tgatgaattc agaaaatgtc gattattacg cgacttttga taattttgga
    45481 gtaggtgtat cgcaagcagc ttatattgaa gagcacttag gattgaaaga aggaaaagga
    45541 ccattcacga ttgaattatt tggtggctcg cctgacgaca acaacgcact aatcaattac
    45601 aacggagtga tgtctgtttt acaaccatat atggacaacg gtcagttggt cgtgccatct
    45661 ggacagacga gtttcagtca aatcgctacg cttcgctggg atggttctac ggcgcaagca
    45721 aggatggata atctgttaag cgcaaattat actgaccaaa cattggatgc tgttttgtcg
    45781 ccatatgatc caataagttt aggaatcatt tcgtcattaa aaggcgtggg gtatgggagt
    45841 gaaagtaaac ctttacctgt gattactgga caagatgcga cagttgctgg cgttaaatcg
    45901 atcattgccg gggaacaaac acaaacgatc tttaaagaca cacgtatctt agcaaaaaat
    45961 acgattgaaa tgatcaaagc aatcagtgat ggagaagagg ttccggtcaa tgatactgaa
    46021 acttatgata atggggtgaa aactgtccct acgtatttag caaatacagt atctgtcgat
    46081 aaagataatt atcaagcaga gctgatcgac accgattatt ataaagaatc cgatctgaaa
    46141 aattaagagg aaccaaacaa ggaactaaag agatctaagt cagagagagg ataagaatta
    46201 tggcggattg tattttaaaa atggcagata tcaccaaaaa attttctgga atccgagcgt
    46261 tagattgcgt gaatctgatg attgaacgtg gggaaatcca tgcattgtgc ggagaaaacg
    46321 gagcgggaaa atcaacattg atgaatgtct tgagcggctt gtatccatat ggctcctatg
    46381 acggggaaat cttttatgat ggaaagctgt gtcaatttaa agatttgaaa gatagtgaag
    46441 aaaaaggcat cgtcatcatt catcaagaat tagcactaag tccttatctt tcaataaaag
    46501 agaatatttt tctcggaaat gaacaagcag aaagaggcgt gattgattgg gaactgacag
    46561 aaaagaaaac atatgaactg ctgaataaag tcggtttgtc tatcgacccg aatcttctag
    46621 tatcccagat tggcgtaggg caacagcaac tagttgaaat agctaaagca ttatcaaaat
    46681 ccgtccgtct gttgattttg gatgagccta ccgctgcatt gaatgaagaa gaaagcgcga
    46741 atttgctgag attgatccga gagttgaaag agcaaggtgt aacctccatc attatttcac
    46801 ataagctgaa tgaaatcgtg gatatcgcag atcgaatcac cattttacgg gatggacaga
    46861 cgattgagac actagaaaat gaaatgatca gcgaagagca tattattcgc ggaatggtgg
    46921 gcagagattt agttgatcgt tatcctgaaa gaaaaccgga tattggagaa gtttatttta
    46981 aagtagaaga ctggacggtc tatcaccctc ttgatcacga gcgaatcata aacgaccaca
    47041 tcaacttgca tttgaaaaaa ggagagattg taggaatagc tggactgatg ggagcgggca
    47101 gaactgaatt cgctatgagt ctatttggac attcttatgg aagcaagata tccggacgta
    47161 tattaaagga aggggcagag atatcagtta agaccgttcc ttcagccatt gcaaacggac
    47221 tcgcttatgt ctcggaggac cgaaaatctt taggactgaa tctgctgatg gatattcggg
    47281 aaaacgtgac gctgagcagt ttggataaaa taagtcaaaa aggagtatta gataaagaaa
    47341 aagaagtaac tgaggcagaa aatttccgga aaaagatgcg tataaaaaca aactccatcc
    47401 atcaaattgt cagtagtctt agtggtggga atcaacaaaa agtcgtttta tccaagtggc
    47461 tgatgacaca acccgatatt ctttttttgg atgagccaac ccgtgggata gatgttgggg
    47521 caaaatatga gatctacacg attatcgaag aaatggcagc aaatggcaaa gctgtctgtc
    47581 ttatctcttc tgaacttcct gaaatattag ggatgtgtga tcgaatctat acgatgaacg
    47641 aaggacgtat tacaggagaa gtctcgcgag aagatgcaga tcaggaagtc ttgatgaaat
    47701 taatgacgaa agaagaggtg ggttaagttg gaacaggcaa aaaatcttac ggataaaaaa
    47761 gagaaaaaac taaatttgaa aagtagagca gctgatcttt ttggtcgcta cagcatggtg
    47821 atcattttag cgggactact tattgcgttt caattgatga cggatggtat tttctggcgt
    47881 ccattgaata taactaacct tgttctgcaa aatagccata tattagtact agctgcaggg
    47941 atgttattag ttgtgctctt aggtcatgtg gatttgtcgg tagggtcagt gatggcgttt
    48001 gttggtgcaa ttgcgggcat gatgatggtc aatcgccaaa tgaacccgtg gatgacagtt
    48061 atcgcatgtc ttgcagtagg cgggctgatc ggcgcatggc aaggtttttg ggtagcttat
    48121 gcagggatac cggcgtttat cgtgacttta gcaggattgc tgatgtttcg tggactgaca
    48181 caagttgttc taggaggcca atctctagca ccttttccag ctgttttcca aaaaatgtct
    48241 acgggttatt taccagactt aagcgaagga agcagccttc acttcttgac attgatttta
    48301 ggaatgatca tcacgatcgt gttagttgtt ggtcaatggc gaacaagaaa tcgtcggaaa
    48361 aatcatttat ttgaagtgga atctccaaca gctttcttgg taaaggcggt atttacagca
    48421 gtcgttgtac taggaataac ttatatcttt gcctcttatc aagggtatcc gattattttg
    48481 atcattcttg gtttgatcgt tgcagcatat gggtttttga caaataaaac agtagcaggt
    48541 cgacagattt atgcgacggg cggaaatcga aaagcggctg aactatcagg gatcaaaaca
    48601 aagcgaatca cgttctgggt attcgtcaac atgggagtca tggcagcttt agctggatta
    48661 gttttagctg ctcgattgaa tgcggcaaca cctcaagcag gtacatcgat ggaactggat
    48721 gcaatggcgg cagtatattt tggtggtgct tcgacttccg gcggcattgg aacaatcatg
    48781 ggtgcgattg tcggtggctt ggtcatgggt gtattgaaca atggcatgtc gattttaggc
    48841 gtcggggtag actggcagca ggcaatcaaa ggattgatct tgcttttagc cgttgtgcta
    48901 gacttgtata ataagaaaaa acgaacaacg taggagaacg ccggttattc agaaaaaaga
    48961 ctgtgacaag acatttgtca cagtcttttt ttcgctaaaa gcgaacattc attgtttcgt
    49021 ttgtatagac taaaaataat atatgttttt ttaccattcg tataaattct ctttttgctc
    49081 ttgatttttt tgcttaattg agttaaatta catagggcgc taaaaaaagc cttgattaaa
    49141 ttaataatgt tcgtgttttg ctggatgtga acatcaggag gataatgtaa tgaaagtatt
    49201 tgattacgaa gatattcaat taattcctaa taaatgtatc gtaaatagcc gttctgaatg
    49261 tgatacaacg gtcacattag gtaaacatac attcaaaatg ccagtagtgc ctgccaacat
    49321 gcagacgatt attgatgaaa caatcgcaga attcttagcg gaaaatggtt atttctatat
    49381 catgcatcgg tttgatgaag cagctcgaat tccgtttatc aaaaaaatga aaaagcgtgg
    49441 attgattacc tcgatcagtg ttggcgtcaa gaaagaggaa tattccttta tagagaagtt
    49501 ggcagaagaa tcgctaaatc ctgattatat cactatcgac atcgcgcatg gacatgctaa
    49561 ctcagttatt gatatgattc agcatattaa aaaatatttg cctgaaactt ttgtgattgc
    49621 cggtaatgtt ggtacaccgg aagccgtccg tgaattggaa aatgcgggcg cagatgcaac
    49681 aaaagtcggg attggtccag gaaaagtatg tattacaaaa atcaagacag gctttgggac
    49741 tggaggctgg caactggcag ctcttagatg gtgtgcgaaa gcagctcgca agccgatcat
    49801 cgctgacggt gggatcagaa cacatggcga tattgcaaaa tctgtccgtt ttggtgcgac
    49861 aatggtcatg attggttcat tgtttgccgg tcacgaggaa tctcctggcg aaacaaaagt
    49921 cgaaaatgga atcgtgtata aagaatactt tggttctgct tcagaattcc aaaaaggcga
    49981 aaaacgaaat gttgaaggaa agaaaatctg gatccaacac aaaggatctt tgaaagatac
    50041 actagtggaa atgcaacaag accttcaatc atctatttct tatgctggag gaagagatct
    50101 ggaagccatt cgaaaagttg actatgtcat cgtgaaaaat tcgattttta atggagacac
    50161 aatctaaaaa ataaataaaa tattaggatg ttgtgaaaaa gttgtcctgc attaagtaat
    50221 aagaacagcc aaacaaaaat agttctttat atgttttgtt tggctggaat tattatcgca
    50281 gatacaagct tttgaacatc attattcaga ataagggact gtgataagat ttttgtcaca
    50341 gtcccttatg ttttgcaaaa aaagtttttc ttttttctcg taaattcggt aaaatcaaaa
    50401 gaaaaaatag acaatagaaa agagaagctt atgtgggaaa aagtagagaa aaaacaagga
    50461 ccgatttatc gtcaaattat ggaacagatt atgagagaaa tcgaaaacgg aaggatcaat
    50521 cctggaagtc aacttgaatc agaacgaaaa ctagggcaac tttttggtgt gaatcgttcc
    50581 accgttgtcc atgcattgga agagcttagg gagcttgggg tactaacgag caaacgtggc
    50641 agcggatggt ttgttaatca gactgaatgg gggaaattcg ctgttccaag aatcgattgg
    50701 cggcaaatga tctctcctcg ttatgagcag acggactggt atgagcaaaa aataagagaa
    50761 gcaaagcaga tccaaaaaac atcgtttctt gatttatacg ctagcgagat gccagatgag
    50821 cttttgccga attttgagtt tccttcttat tctcttgaag aaatcttagc ggaagaaaaa
    50881 gaaatggatc ttttaggcta tactccgtta cgtaacaaaa tcaaacggta tttggaaagt
    50941 aaatttgaat tggcattttc ccccaaccag cttcttgtga ctggtggcgg tcagcaagca
    51001 atctttttga ttttacaaac gattctttca gctggagagg ggatagctgt agagtcacct
    51061 tctttttttt accgattacc tcttttcaaa gcttcaggaa cgcgtttatt tgggataccg
    51121 atggatgaag aagggatcga cttagaaaaa ctgaaagaat cgatccataa aaataaattg
    51181 aaagcagtgc ttatcaaccc gaattttcaa aaccctacag gaaaagtgat gagtcaaaaa
    51241 cgacgagaaa agctagtaga attgtgtcga cagtatcggc ttccaattat tgaagacgat
    51301 gtatttacgg atctttcatt tctaaaccaa gaagaagtcc ttcctatacg aacgattgat
    51361 ccggataatg tgttatacgt cggttcgtta tctagggttt taggaaaaac aacgaaaatc
    51421 ggttgggtga taggacctga gacttttatc tcgcaactag ctcaagcgca agaaatgatg
    51481 gagttttcga tgaatatcct tacacaaatc acagtggcta atgtatttaa tgaagcaatt
    51541 gatttgaaaa tgcaagatgt aagaaaaaag ttgaaagaaa aaagccgact gataagcgct
    51601 tggggagatt cacaatcgtt tttcaagctt cattcaattt taggtggtta ctatgcctgg
    51661 attacttggg atgggaaaaa actagacccc gaactagcag aaaaagtagt agatatgggt
    51721 cttggaattg caccaagtct actttttggg caagatataa atggaatcag aatcaattac
    51781 agtaggctgg atgaaaacca aataccgttg ttttattcaa agatgaaaat tctagaaaag
    51841 tggttaacag aataatttca caaagtaaat agattaaaga atataacagc ttggatttaa
    51901 atttcaagct gtttcttttt tttcggaaaa agaatacgat ataaagaaac taagaaaaaa
    51961 ccattatttg tacgtccgtt cacgatgtgt aaataaaata aatgtttgca acaaaactgt
    52021 ataacaccaa cgtttttaaa tagaaacaga tggttgtaag tttgattaga aaatagaaaa
    52081 aatgcagatg aaatataaaa cgctttcctt attgtgattt tattcactaa tgctgttaat
    52141 atgcggattg gcataatttc ctttcatcgt gaaaatttgt gcttttagct tgtatttctc
    52201 ataaatccat gataatttat taatgaagac ggttaaatag tgatacagtt ttattcactg
    52261 tttcaatcgc agaatgaaga gattttctta tagatgagag aggaatgtgt gacaaatggc
    52321 aaacaaaaaa gcaccgatcg acttccaagc actgctagat gaagtcaatg cagatttccc
    52381 agtatatcaa gcacttgatc aagatggaaa cgtagttaac aaagatttag tacctgattt
    52441 atccgatgat gaactcgttg aattaatgac tcatatggta tggtctcgtg tattagacca
    52501 acgttcaaca gcgctgaacc gccaaggacg cctaggcttt tttgcaccaa ctgctggaca
    52561 agaggcaagt cagctggcta gtgcttacgc atttgataaa gaagatgttc ttcttccagg
    52621 ttatcgggat gtacctcaac tagtaaaaca tggtttacct ttatctcaag cttttttatg
    52681 gtctcgagga catgctgcag gtaattctta tccggaagaa ttgaaagctc taccaccaca
    52741 aattatcatc ggtgcacaat atgtacaagc agccggtgtt gcattaggtt tgaagaaacg
    52801 aaacaagaaa aatgtcgtct tcacttatac tggtgatggc ggttcatcac aaggtgattt
    52861 ttacgaagca atcaatttcg ctggcgctta ccatgcaaat gccgtatttt atatccaaaa
    52921 caacgggtat gcgatctcta cacctcgtga aaaacaaact gcagcaaaaa cacttgcaca
    52981 aaaagctgtc gcagctggga ttcctggtat tcaagtcgat ggaatggatc cgcttgcagt
    53041 atatactgta tctaaaatgg cacgggaatg ggcgatcagt ggaaatggac cagtcctaat
    53101 cgaaacactg acttatcgtt atggaccaca tacactatct ggtgacgatc caacccgcta
    53161 ccgtacaaaa gacacagatg acgattggca gaaaaaagat ccattgatcc gcttccgtaa
    53221 atatttgacg gaaaaaggac tttggtctga agaaaaagaa gaacaagtca tcgaagcaac
    53281 taaagaagaa atcaaagcag ctattgctga agccgacaaa gtaccaaaac aaaaggtttc
    53341 agatttcttg aaaaacatgt tcgaagtatc tccacaatca atcaaagaac aaattgcaat
    53401 ttatgaagcg aaggagtcga aataatcatg gcacaaaaaa caatgatcca agcaatcaca
    53461 gatgccttag cacttgaact agaaaatgac gagaatgtcg ttgtttttgg tgaagatgtt
    53521 gggaaaaacg gaggagtttt ccgggcaact gaaggattgc aggaaaagtt cggagaagac
    53581 cgagtattcg atactccttt agctgaatct ggaatcgcag gattgagttt cggtcttgcg
    53641 ttagaaggat ttcgtccagt tcctgaaatc caattctttg gttttatttt cgaagcaatg
    53701 gacgaagtag tagctcaaat ggctcgtaca agatatcgaa tgagcggcac tagaaatcta
    53761 cccatcacaa ttcgttcgcc atttggtggc ggtgttcata caccagaatt gcattcggat
    53821 aatttagaag gtttgatcgc tcagtctcct ggtattcgag tagtgattcc ttccaaccct
    53881 tatgatgcaa aaggactatt gatttcttct attagaagta acgatccggt tgttttccta
    53941 gaacacatga aactatatcg ctcattccga gaagaagtgc cggatgaagc ttacgaagtt
    54001 cctttggata aagcagcagt tactcgtgag ggaacagatg tatctattat cacatacgga
    54061 gcaatggtgc gtgaagcaat caaagcagca gacaatcttg caaaagaaaa tatatctgtt
    54121 gaaatcattg atcttcgtac agttgcgcca ttagatgtag aaacgatcat tcaatccgtg
    54181 gaaaaaactg gtcgcgtagt tgttgtccaa gaagcacaac gacaagcggg cgttgccgcg
    54241 caagtagttt ctgagatctc agaacgcgct atcctttcat tagaagcccc aatcggacga
    54301 gtttctgcac ctgatacagt cttcccattt ggacaagcag aaaatgtctg gttgccaaat
    54361 gcaaaagata tcgaagacaa agtaaaagaa atcgcggaat tttaagaaaa gaagggacga
    54421 aacaaagtca tggcttatca atttaaattg ccggatatcg gtgaaggaat cgcagaaggt
    54481 gaaatcgtca aatggttcgt caaaccagga gatacgatca atgaagatga tacattacta
    54541 gaagttcaaa atgacaaatc agtagaagaa atcccgtcac ctgttacagg aacggttaaa
    54601 aatgtgattg ttccagaagg taccgtagca aatgttgggg atgtattagt agaaatcgat
    54661 gcaccaggac acgaagacaa tgaaggggac agtggtgtag ccgcagaatc ccaaactcca
    54721 gctaaaccgg ctgctgaacc cactgttgat acagaatcag ctggatcgtc aagcgaagga
    54781 gtcttccaat ttaagttacc ggatatcggt gaaggaatcg cagaaggtga aatcgtcaaa
    54841 tggttcgtca aaccaggaga tacgatcaat gaagatgata cattactaga agttcaaaat
    54901 gacaaatcag tagaagaaat tccgtcacct gtcacaggga cagttaaaaa cgtgattgtt
    54961 ccagaaggca ctgtagcaaa tgttggggat gtattagtag aaatcgatgc tcctggacac
    55021 aatagtgcac cgtcaactag tgcgccatca gctgaagcac caaaagaaaa agtggaaaca
    55081 tcaggttcag caagtgtcgt tgaagctgcc gatccaaata aacgtgtatt ggctatgcca
    55141 tctgttcgtc aatttgcccg tgaaaaagat gtagatatct cacaagtaac tgcaactgga
    55201 aaaggtggca gagtaacaaa agaggacatc gaaaacttct tggcaggtgg tccaagcagt
    55261 gcaccagcga aatcagaagc gccagaagcg gcagcaccga aagaagcagc tcctgcagca
    55321 gaaagcaaac cagctgcacc agcaaaacca ttcaagtcca acttaggaga cttggaagaa
    55381 cgtgtagcaa tgacgccaac acgtaaagct atcgctaaag caatggtcaa cagtaagcat
    55441 acagctccac acgtgacatt gcatgatgaa gtagaagtgt ctaaactatg ggataatcgg
    55501 aaacgattta aagaagttgc tgctgcaaat ggtacgaaac ttactttctt gccatatgtc
    55561 gtgaaagctt taacagcgac tgttaagaaa tacccagtat tgaatgcatc tatcgacgat
    55621 gcaaaccaag aaatcgttta taaacattac tacaatatcg gtattgcaac tgatacggat
    55681 cacggactat atgtaccaaa tgtgaaagat gcagaccgca aaggaatgtt tgctattgca
    55741 gacgaaatca atgagaaagc aaaattagct catgacggca aactatctgc agaagatatg
    55801 cgcaatggaa caattacgat cagcaatatc ggatctgtag gtggcggttg gtttacacca
    55861 gtcatcaact atccagaagt agctatttta ggtgtaggaa cgattgcaca acaaccaatc
    55921 gtaaatgctg aaggcgaaat cgttgtcggt cgtgtgatga aactgtcatt aagtttcgat
    55981 caccgtatcg tagatggagc aactgcgcaa caagcaatga ataatatcaa acgtctttta
    56041 gctgatccag agctattaat gatggaagga tgataaacaa atggtagttg gagattttgc
    56101 tgtagaatta gatacagttg taattggtgc gggacctggt ggctatgtag ctgccatccg
    56161 cgctgctgaa atgggacaaa aagtagcaat cattgaaaga gagtatattg gcggtgtttg
    56221 tttgaatgtc ggctgtattc cttcaaaagc actgatagct gcaggacacc attatcaaga
    56281 gtcactagat tctgaaatgt ttggagtaac tagtgaaaac gtcaaactag attttgcaaa
    56341 aacacaagaa tggaaagaaa acaaagttgt ccatacgttg acttctggtg ttggtttcct
    56401 attaaaaaaa cataaggttg aaacaatcga aggagaagcg ttcttcgtag atgatcacac
    56461 actacgcgtc attcatccag attcagctca aacttattcg ttcaataacg caatcatcgc
    56521 aacaggttcc cgtccaatcg agattccagg atttaaattc ggaggacgtg tgttagattc
    56581 tactggcgga ttagcattga aagaagtacc taagaaattt gtgatcatcg gtggcggtgt
    56641 gattggtgcc gaattgggcg gagcgtacgc aaacttagga gcagaagtaa cgattcttga
    56701 aggctcacca caaattttac caacttacga aaaagatcta gtaaaattag tagaagatga
    56761 tttcaagaaa aaaggcgtaa cagtcgtaac gaatgcaatg gcgaaagaag cagtcgataa
    56821 cggtgacagc gtgacagtga aatatgcagt ggacgggaaa gaagaatccg ttactgctga
    56881 ttatgtgatg gtcactgtag gtcgtcgtcc gaatacagat gatatgggct tagaacaagc
    56941 gggtgtcgaa gtaggagaac gaggattgat cacggtggat aaacaaggac gtacgaacgt
    57001 acctaacatc tatgccatcg gtgatatcgt tccaggggcg gcattagcac acaaagccag
    57061 ttatgaagca aaaatcgctg ctgaagcaat ctctggcaaa aaagtagctg tagattataa
    57121 agcaatgccg gcagtagcct tcactgatcc tgaattggct tctgtaggta tgacgatcaa
    57181 agaagcaaaa gatgctggta tcgaagcaaa agcatataaa ttcccatttt cagggaacgg
    57241 tcgggcatta tcgcttggta aaacagaagg attcatccgt ctagttacga cgatcgaaga
    57301 taacgtgctt attggcgcgc aaatcggtgg agtcggcgca agtgatatgg tctctgaatt
    57361 agctttagca atcgaatcag gaatgaatgc tgaagatatc gccttgacga ttcatcctca
    57421 tccatctctt ggcgaaatcg tcatggatgc ttcggaatta gcattaggtt taccaatcca
    57481 tatttgattt gaataataca ggcgaaacgc aactgaaaaa tcagttgcgt tttgtttttt
    57541 agaattatca ataatattat tatgtaaact taaaaatatg acaacttctt ggtttattac
    57601 cacttcctat gctacactga agaggaatat gaaataggag agttgaagat gcaagaatat
    57661 cagtatccac ttgatttaga ttggacaaca gacgaaatgg tcattgtaat gaagatgtgg
    57721 gaagatctgg aacgtgcgaa tgaaaaaggg ataaacaatg aagctttttt gaagacttat
    57781 cagcagttta aaactgttgt caaatccatt ggtgaggaaa aacgattggg acgggaattt
    57841 gaaaaagctt ccggttattc tttatatcgt acagtgcagc aagcaaaaaa aaatccaaac
    57901 aaaactttga gaatgaacgg gtgagataaa tggaacagat gattgatcat atcaagtcct
    57961 ggcttgaaga agctggtgag aaaatcaaat cttctcaaca aacattaaca atctcgcaaa
    58021 aatcaaatcg aacggatcta gtaacaaatg tcgatcgaaa catccagaat tttctaatcg
    58081 aaaaaatcca ttcctttgat ccagaggcga aaatattggc agaagaagaa gggtgtaatc
    58141 aattatcgga tctcgatgga agagtcttta tcatcgatcc tattgatggc accttgaatt
    58201 ttgtgattga aggggaaaat ttttgtatta tgctcgctgt ttatgaatcc ggcgttggaa
    58261 agcttggttt catttatgac gtgatgagaa atgaactttt ttggggtgga aaaggaatag
    58321 gcgtgtataa aaatgatgag ccattgccta gaccagcaaa taaatcctta tcggaaggct
    58381 tacttggtgt gaatagttat cttttcggtc ataataaata tggaatccga gaaattgcgg
    58441 aaagcagcat ggggacacgt atgtacggat gcgctggtct tgaactagtg gcaatcttga
    58501 aaggaaacca cattgcttat atttcaaacc ttagcccttg ggattatgca gcgggaaatg
    58561 tcttattgga agagtttggt atggtaacaa gcggtctttt tggtactcct ttgacatttg
    58621 aaaaaaggga atactattta gcagggactt cttgtgcata tgcagaaatc gcagaccttt
    58681 tgaaaaaaga aggctgaaaa aaaggtagaa atcattcaca aaaatgataa aaacgagcaa
    58741 aagattaatt ttctcgaaaa aatgaaaaca ttttactata tttcatgaaa aaagtctgct
    58801 ataattagca gtcgagtaaa atttgccctg tcataaagaa ccaataaaaa aggagttagt
    58861 tacattgaat tatagaaacg atatccgtaa cgtcgcaatt attgcccacg ttgaccacgg
    58921 aaaaacaaca ttagttgatg aactattaaa acaatcagat acattagacg cacatacgca
    58981 attgcaagaa cgtgcgatgg actcaaatgc gctagaaaaa gaacgtggta tcacgatttt
    59041 agccaaaaac acagctgtag actataaagg tattcgcgta aacatcatgg atactccagg
    59101 acacgcggac ttcggtggcg aagtagaacg gatcatgaaa atggttgacg gcgttgtgtt
    59161 ggtcgtagat gcgtatgaag gaacaatgcc tcaaacacgt tttgtattga aaaaagcact
    59221 ggaacaacat atcacaccaa tcgttgtcgt aaacaagatt gacaaacctt ctgctcgtcc
    59281 tgagcatgtc gtagatgaag ttcttgaact attcatcgaa ttaggagcag atgatgatca
    59341 gttagacttt cctgtgattt atgcatctgc actaaatggt acgtcaagct tgtcagatga
    59401 tccagcagat caagaaccaa ccatggcacc tatttttgat acaattattg aaaaaatccc
    59461 tgctcctgtc gataacagcg acgaaccatt acaattccaa gtttcattac ttgactacaa
    59521 cgattatgta ggtcgtatcg gaatcggacg tgtatttaga ggaacaatca aagtaggaga
    59581 ccaagtagca ttaattaaat tagacggaac agttaagaaa ttccgtgtaa cgaaattatt
    59641 tggtttcttt ggactaaaac gtctagagat tcaagaagca aaagctggag acttgattgc
    59701 tgtctcaggt atggaagata tctttgtcgg tgaaacagtt actcctgtgg atcatcaaga
    59761 tgcgttacct atccttcaca ttgacgaacc aacattgcaa atgactttct tagttaataa
    59821 ttcaccattt gccggtcgag aaggtaaatt cgtaaccgct cgtaagattg aagaacgttt
    59881 gatggcagaa cttcaaacag atgtatcctt acgagttgaa ccaacaaact ctccagatgc
    59941 atggacagtt tctggtcgtg gagaattgca tttgtctatc ttgattgaaa acatgcgccg
    60001 tgagggatac gaattgcaag tatctcgtcc agaagttatt gaaaaagaaa tcgacggtgt
    60061 aaaatgcgaa ccgtttgaac gcgtgcaaat cgatacacca gaagaatata tgggtagtgt
    60121 tatcgagtcc ttgagcttac gtaaaggtga aatgcaagat atggtccata caggaaacgg
    60181 acaaatccgt ttgacattct taacacctgc ccgcggtttg attggttatt caactgaatt
    60241 cctttcaatg acacgtggtt acggaatcat gaaccatact tttgaccaat atctgccaat
    60301 gcttcctggt caaattggcg gacgtcacca aggggcgctt gtttcgatcg atacaggaaa
    60361 agcaacgact tattctatca tgagtatcga agaacgcggt acagtcttcg tagaaccagg
    60421 tacagaagta tatgaaggaa tgattatcgg ggaaaacagc cgtgataatg atttgactgt
    60481 caacatcacg aaagcaaaac aaatgacaaa cgtacggtct gctactaaag accaaacatc
    60541 tgtcatcaaa aaaccaaaac aattgacgtt ggaagaatct ttggaattct tgaatgatga
    60601 tgaatattgc gaagtaacac cagaaagcat tcgcttgaga aaacaaattt tagagaaaaa
    60661 tgctcgtgaa aaagcatcta aaaagaaaaa ataatctgta aaaggatgaa gagagaaacc
    60721 agcttgttat aaaaaatagc tggtttcttt ttttgtccaa ctcttttctg tggagaagtc
    60781 atctttgtca atggacataa attcagaaaa tacgccgaaa gacgtttaaa agacttttaa
    60841 aaaacttatt tctagagagt ttttgtttca taataagata atgtttcacg aaaggagatc
    60901 aacttgtcct tcgaagaaaa tcgaagtaaa atggcaagag aaaggagcgg gaagatgaac
    60961 gacttattaa aacaatactt tggctatgat gagtttagac ctggtcaaaa agaaattatc
    61021 cagaaagtca ttgatcagga aaatgtatta ggaatcatgc ctaccggaag tgggaaatca
    61081 atctgttacc agcttccagc tttactatta gatgggctta ctgtggttgt gtccccttta
    61141 atctctttga tgaaggatca ggtggacgcg gcgaatcaat taggtatacc cgccactttt
    61201 atcaatagct cgttagatgg ttacgaaaca gctagacgct tccaagaaat cgatagacag
    61261 cagtaccgtt tactttatat cgctcctgaa cgttttatca tgccggattt tatccaagca
    61321 atgaagcgat ggaacgtccg tatgatcgct attgacgagg cacattgtat ctctcagtgg
    61381 gggcatgatt tccgcccaag ttatttacag atggcgaacc agctagatca actgccaaat
    61441 cgtcctgtaa ttgttgcttt gacagctaca gcgactgtgc aagtagctgc tgacataaaa
    61501 agacttttaa aaattccaga aaacaaccat attcagactg gttttgaacg agaaaatcta
    61561 cgatttcaag taattaaaga tcaaaaaaaa gaacaatatc taatcgaata tttaaagatc
    61621 aacaaaaatc aatctggtat catttatgca gcaacgagaa aagaagtaga ccgtctctat
    61681 catttgctta aaaaattcga tttttccgtg ggtagatatc atggtggtct aaatgagaat
    61741 gagcgaacag aaatgcaaga agccttttta tacgatcgct tgcagctgat tgtagcgaca
    61801 aatgcatttg gtatgggtat aaataaaagc aatgtccgtt ttgttatcca ttatcaaatt
    61861 cctggctctc tagaagcata ctatcaggaa gctggtcggg cgggaagaga tggtctttcc
    61921 agtgaagcca tcctgctttt ctctcctcaa gatatccaag tccaaaagtt ttttatccag
    61981 cagtcccaaa gagaagaagg gcaaaagcaa aaagaatatg aaaaattaaa agcgatgaca
    62041 gaatacgtgt atattgagtc ctgtcttcag caatacatcc taaactattt tggcgagaca
    62101 agcagtccgt gcaaccgttg tggtaactgt ttagatgaca gagaaatagt agaagtaacg
    62161 acagaagcac agatggttct ttcttgctta aaacgtatgg gtgagaacta tggtaaacaa
    62221 atgctgatga aagtcttagc aggatcgaaa gaacaaaagc tgcaagcttt aggttttgga
    62281 catttatcta cctatggact gatgaaaaac caatcgcaaa aagaaacgat gcagttaatt
    62341 gaatatttga tatcgaatgg ctatcttctg accatcaatg gtgagtatcc tgttttgaaa
    62401 gtaacagaaa gagggataca agtattgaaa ggacaagaat ctgtttatcg aaaggaaccc
    62461 aagaaagtcc agcagctatc ggatgaagaa accgatacac tatttgaagt gctgcgggaa
    62521 ctgcgaacgg atctagcatc agaagcaggc gttcccccgt atgtcgtatt ttcagattcc
    62581 acattgaaag aaatgagccg aatacgtcca tcaagcagat tggagatgct tcagataaaa
    62641 ggagttggcc aaagcaagct tgataaatat ggcgaagcct ttttatctcg gataaaaaat
    62701 gcggactcta acgttctgga taagtagaaa gaacgaatac gtaaaaatga ataaaaacaa
    62761 ctggttaaat ctgttttttt tgctccttat gaaaatcaaa tatgttataa ttaaggaatt
    62821 agaatgggag gtaagcacag atggattctt tgtcgaaaaa acttgcgatc caagtattaa
    62881 atgatgaagc agaacggatc aaacgattaa tccgaaatca aaacaacagt ctctgtatct
    62941 cacaatgcaa agccttcgaa gaagtggtag atacacagat gtacggcttt tcccggcaag
    63001 tcggttttgc tgtccgtata ggagtaatcg gaaagaacga gggccagctg atgttaagtg
    63061 agctagaacg agaattgaat cagttatata gtgaggttta ccaagaaatt tatgataaaa
    63121 aagaagttgg caaggaggaa tagacttgcc taagaaagtg aaaaaaaggc atctattaga
    63181 ctatagcatc ttgattccct atttaatttt atgtgtattg ggattgatca tggtatatag
    63241 ttctacgtcg tatctattat tagaaaacgg acaaaatccg tccgcatcag tcattaacca
    63301 gagtattttt tgggtgctta gcttgattgc tatcgcacta ctttataaaa tgaaaacaga
    63361 tgttttgaaa aaccagcgtt tgatcatggc agccatcgca gttttgacga ttttgctgct
    63421 gattgttgtg ttttttggta aggagatcaa tggggcgaaa ggttggctgc aaattgcagg
    63481 attctcgatc caacctgccg aatacctaaa aatcatttct atctggtacc tgtctctgac
    63541 tttatctaaa agacaaaaca gtgtacaaaa agattttctt gggactgtga aacgtccact
    63601 tgcaatggtt atcggactta ctgcattagt cgctattctt cccgattttg gtaatgcggc
    63661 agttatcttt ttgattattt tggttctgct tttagccagc ggagtcaatt atgtgtatac
    63721 tttgattgta ggagtaggag gcttttgtct aagtactttt acgatctggc tgatcaatat
    63781 cacaaacgga aagatcttac ccggacgttt acagtatatc tataatcgat ttgctattta
    63841 ccaaaatcct ttctcagacg aattaaataa agggcatcaa ctagtcaacg gctactatgc
    63901 catgttcaac ggaggactat ttggcagggg attaggtaac agtatccaga aaaaaggatt
    63961 tttacaagaa gcacaaaccg attttattta tgcaatcgta gtggaagaac tgggagtgat
    64021 tatggggatt ttgatattag cgttgctatt ctttatgatt gtgcgcattg tccttgtagg
    64081 tatccgttca aaagatccgt tcaattcgct tttgtgcatt gggatcggcg caatgttttt
    64141 gatccaagta tttgtcaatc ttggaggaat tactggcgtt attcctttga cagggatcac
    64201 gttcccattt ttaagtcaag gaggctccag tttgctgatg ctttcgattt gtgtcggctt
    64261 tgtcctcaac atcagtgcgg atgaaaaacg caagagctta ggtttataat aaaaaaagat
    64321 gtaggagtgg ttcgatgaaa aaagttttag ttgctaacag gggtgaaatc gctgtacgtg
    64381 tatttcgtgc ttgtacagaa ttaggaatta aaactgttgg aatctatgca gaagaagatg
    64441 aatattccgt acaccgtttc aaagcggatg aagcatattt agtaggacaa ggaaaaaaac
    64501 cgattgatgc gtatctagat attgaaggga tcatttctat cgcgaaagaa tgcggagctg
    64561 acgctattca tccgggttac ggtcttttgt cggaaaactt gaattttgcg caacggtgcg
    64621 aagaagaagg catcattttt gttggaccca aactgcatca tttagatatt tttggagata
    64681 aaatcaaagc aaaagctgca gctatcgaag cggggattgc ttcgattcca ggcacagatg
    64741 gaccaatcgc ttctatagac gatgccttgg agtttgcgaa acaatacggt tatccgatta
    64801 tgatcaaggc agctctaggc ggcggcggac gcggcatgcg tgtagctcac gatgaaaaga
    64861 gtgcaagaga gggctacgaa cgtgcgaaaa gcgaagcaaa agcagccttt ggtagtgatg
    64921 aagtgtatgt tgaaaaatac atcgctaatc ctaagcatat cgaagtacag attctcgggg
    64981 atacgcatgg aaacgtcatc catctttttg aaagagattg ttctgttcag cgccgtcacc
    65041 aaaaagtagt agaagtagcg ccttgtgtct ctatgaatga acagcaacgt caaaaaatct
    65101 gccaggcagc tgtccaattg atgaaatatg tcgggtatgt caacgcaggt acagtagaat
    65161 ttcttgttga aggagatgat ttttacttta tcgaagtcaa ccctcgcgtc caagtggaac
    65221 atacgatcac agaaatgatt acagatattg atatcgtaac aacgcaactg ttgattgctc
    65281 aaggtttgga tctgcataaa gaaatcggat tgcctcagca agaaggaatt aaattaaatg
    65341 gttcagccat tcaatgccga atcactacag aagacccgtt gaataacttt ttaccagaca
    65401 cagggaaaat cgatacgtat cgttcacctg gtggttttgg cgtacgtctg gatgtcggaa
    65461 atgcttacgc gggctatgta gtgacaccgt attttgattc attgctagta aaagtatgta
    65521 cgcatggtgc tacctttgaa acagccattc agaaaatgga acgttgcttg aaagaattta
    65581 gaatccgtgg agtgaaaaca aacattccat ttatgctcaa tgtcatcaca catcctgaat
    65641 tccaatcagg aaatgcaaaa acaaccttca ttgatagtac agcaacatta tttgaatttc
    65701 ctcgactacg agaccgcgga aacaaaacga tgaaatatat cggtgaaatt actgtgaatg
    65761 gttttccagg tatcgaaagt ggcgaaaaac cattttatga agaaccacgt atgcctaaag
    65821 atctaatcac aagaactgat tacgtaacgg ctaagaatgt attagatgcg aagggtgccg
    65881 atgctttggt cgaatggatc aaaggacagg aaaatttgtt gttgacggat acgacattta
    65941 gagacgccca tcaaagtctg ctagcaactc gtgttcggac aaaggatttc aagcagattg
    66001 cacgtttgac tggagaaggt cttcctgaac tcttttcaag tgagatgtgg gggggagcaa
    66061 catttgatgt ggcttaccgc tttttgaatg aagatccttg gcaacggttg agaaaaattc
    66121 gttctttaat gcctaatacg ttgctgcaaa tgcttttcag aggttcaaat gccgttgggt
    66181 attctaatta tcctgacaat gtattggtgg aatttgtcaa agaagcagca gcgcaaggaa
    66241 tcgatgtatt tcgaatcttt gatagtttga actggactcc acaaatggaa aaaagtatcc
    66301 aagctgtacg ggatactggt aaaatcgcag aggcagctat ttgctatact ggcgatatca
    66361 acgatccaag tcgggcaaaa tacaatgttc agtattataa agacatggca aaggaactag
    66421 aacaactggg tgcgcatatc attgcaatca aagatatggc tgggctgctt aaaccgcagg
    66481 cagcttatcg tttgattagt gaattgaaag aaacgacaga tctgccaatc catctgcata
    66541 ctcatgatac cagcgggaat ggaatcatca cttattctgc tgcttcaaaa gcaggagtag
    66601 acatcgttga cgtggcaatg agtgcaatga gtgggaatac tagtcagcct agcatgagca
    66661 gcttgtatta tgcactggtc aatggtccac ggctgccaga aatcacgatt gaaaatgcac
    66721 agaaattaaa ccattattgg gaagatgtgc gcatgtatta caaaccgttt gaaaacggct
    66781 tgaatgctcc tgaaacagaa gtatatatgc atgaaatgcc aggaggacag tattcgaatc
    66841 tacagcaaca ggcaaaagct gtcggattag gtcatcgctg ggatgaaatc aaacaaatgt
    66901 atcatactgt caacttgatg tttggcgata tcgttaaagt aactccatca tctaaagtag
    66961 ttggagatat ggcactattt atggtccaaa atgatcttac agaagaagat atttatgaaa
    67021 aaggcgaaac actaagtttc cctgaatctg ttgtgacttt cttccaagga gaactagggc
    67081 aacccgttgg cggatttcct aaaaaattgc aaaagattat tcttaaaggc agacctgcat
    67141 tgaatgaacg tccaggtctt ttggctgaat cagtcgactt caacgaagta aaaaaagaat
    67201 tggcagaaaa aattggctat gaaccaaaac aagacgaagt attaagctat ttgatgtatc
    67261 cgcaagtctt tttggactat caaaaagcat ataatcaatt tggcgatgtt acattactgg
    67321 acacaccaac atttttccaa ggtatacgtt tgggtgaaac aatcaatgta caaatcgaac
    67381 gcggaaaaac gcttatcatt cgtttagatg aaatcggaga acccgatatc gaagggaatc
    67441 gcgtactgtt cttcaatttg aatggacaaa gacgtgaaat cactatcaaa gataattcga
    67501 tcatcagtac ggtccaaacc cgattgaaag ctgaaccgac aaaccgagaa caaattggag
    67561 caacgatgtc tggttctgtt ttggatgtat tagtgaaaaa aggcgataac gttaaaaaag
    67621 gtgatacgtt gatgattact gaagcgatga aaatggaaac ggccatcgaa gcacgttttg
    67681 acggagaagt cgcacatgta tatgtgtctt caggagacac tatttcgtct ggtgaccttt
    67741 tgattgaagt gactgagaaa taataagcga aaggtgggga aacatgaaaa gactgggttt
    67801 gttttggctg attctgctat tagttattgt ggcattctac atggaaccag tcttgtttcc
    67861 gccaaaactt gaagtgaagg aaaactctga tcaacaaata tcaaaccaaa tgacaacgtg
    67921 gaaataccag gagcttaaag caactggttt ttctacttat atcggaaaac cgatcgatca
    67981 gctggaaaag gattttggcc aagcctatga tcgattaact agcggatttg gttttgaaac
    68041 aagatattat gaaaataaag ctagtcatct cacatttgaa gcgaacatcc aagacagcag
    68101 agtcacaact gttaaagtac tgcaaacagg gcctgctgat atcgcgccgt tttatttagg
    68161 gatgacgatg caagatctta cgaaaatcac tactatctat actaatttca cttttgagta
    68221 ccatgataca gaagttggaa tcgaactaat ggaagaggat atgaattatc gtccgctaat
    68281 tgcttttgat aacgacactt ttgctatttt attttttgat caaacaacaa acggcctatt
    68341 ttcagttgtg tatttagata aagatagtct tttgaaacta ttgccatatc aagtgtttgg
    68401 tgaaggcctt ccacattatc aagcagataa atcggctgat tgggaatcaa tcaatcgcgt
    68461 taaagaacga aaaagtacga ctttattaaa tatgcttaga agagaagacg aacttcccat
    68521 atacaatcaa acacttgcct ttcaaaacca cagtcagctg ctactacatg attttttgac
    68581 aaggcctgag gaaatattgt cagaggaacg tatggctgat tggcaaaaat cgctaggcag
    68641 tgctcaaacg acagtgaaat tctcactgac gaatgatgaa ttggagaagt taatcagtaa
    68701 acaaaaatta gaaaatacta gtgggctatt tattcatccg gcgattgacg ctactttttc
    68761 gtttttatat tggtacagcg atccttacaa ccatgaccgt ttcatggacc cgggaacgga
    68821 ttctgtaggc attgcttttt ctaaagaaaa tatgttagtc ttattgcaag aagaaaacgg
    68881 agaaagtagt gatagcagtg atcgttaacg aagagctgtt tcaactggag gatcaatgcc
    68941 tccaagtcgc tgaaatgata aaaaagagta aaacgatggc ttcttatctt gaacataaac
    69001 agtcaatgga agaatctgca aaagtttgcc agttgaaaga agcgttcttg tctaagaaag
    69061 aagcatttga aaatgtagcg gcatatggtg agtatgcacc tgattatcgt gaaaaaaaac
    69121 gagctgttcg aaaagcaaaa agacagcttg atatggatga gcatgttgca gctttccgat
    69181 atagtgaaac acagctgcaa agtctgttgg atcaaattgt ccaagtactt gccgaagaag
    69241 tttctaatga aatcaaagta gatgcaggta gcccgttttt tgaaacgaaa aaacactttg
    69301 gatgcggagg taattgtcat gcaattagac aatagtgaat ttgaaataac aaaacgacgc
    69361 gggctgatcg tatgggtcta cagcttaaaa caattaaaga atctgaaaaa gttcggcttg
    69421 ttgcattatg tctcaagaag aatgaaatat gtcgtgcttt acttaaatga agagacattg
    69481 gaagaaacag aagaacgaat ccgtaaatta cattttgttc gaaacgtaga acgttcttac
    69541 cgaccggata tcgaaatgaa ttttgccgaa aaaataggaa acaaaacagt attaaaagac
    69601 gaaggtttcg aaattgaaga gctaaataca gaaatccgat tagctgaaca tctttaaaaa
    69661 tctcttatga gcagttttta aataaaaaat aacttggata acgatcgatt tttgatcatt
    69721 gtccaagtat ttttttggta tactagtacc agtaccattt ctaaggagtg agtagatgcg
    69781 gataatatca ggaaattatg gcgggcgccg tttaaaaagt ttagcagggg cgaacaccag
    69841 accaactaca gataaagtaa aagaatccat attcaatatg attggaccat attttgatgg
    69901 agagacagta ttggatctgt ttgcaggaag cggtggttta gctatcgaag cagtatctag
    69961 aggctgcagt catgcggtat gcgtagacaa aaattaccaa gcgctaaaaa tcatcaaaga
    70021 aaacatcgaa attacgaaag aaccagagaa atttaccaca ataaaacttg atgcggacaa
    70081 agcaatcgaa cggctagcag cggagaaaca acaattcgac tacttgtttc ttgatccgcc
    70141 atatgcaaaa caaaaaataa tcgatcaaat ccataaaatg gaagaactgg gacttttttc
    70201 taaaaccgca gttatcgtat gcgaaacgga taaaacagtc gaattgccag aaactgtggc
    70261 tggattccat caaataagaa aacagattta tggcataacg gccgtaacta tctatagaaa
    70321 agagggaaat caatgaaacg tgcattattc ccaggaagtt ttgatccttt tacaaaaggt
    70381 cacttagata cggtggaacg tgcggctaaa ttattcgatg aggtagtaat tggtgttttt
    70441 atcaatactt caaagaaaag tttgtttccc ccagaagaaa gaatgacatt gatcacaaaa
    70501 gctgtttctc atcttcctaa tgtaaaagtc atgcatcaag aaaatcaatt gacggtagaa
    70561 acagcaaaag agattggtgc agatgcgttg atcagaggca tacgaagtat caaagacttt
    70621 gaatatgaac gagagattgc tcagatgaat catcatctat atgatgaatt ggaaactgtc
    70681 ttcttattag caaaaccaga atatagtcac gtgagttcca gtattttaaa agaagtactg
    70741 cactttggcg gagatgtgag cagctactta ccgccagtga tcaacgaagc tttagctgga
    70801 aagcgtgaat caaatgaaag ctaaacaatg gttgaaaaca gtactgatta tcatatttgg
    70861 aatgttgatc attggatttg tagtagttcc cttgccgtat tatgtagaat caccgggagc
    70921 cacgatcgat ctgaaagaac taattacggt caatgataag gaagataaag gttctgggtc
    70981 tttctctctt acctctgttg gtatcaggca agctacaggt ttcttagctt taaaagctaa
    71041 aatgacccct tttgaagaat tgatcagtga agaagaattg actggtgggg cgacaaatga
    71101 agaatataat caaatgcaac agtattatat ggattcttcc caaaatgcag caatcgaaca
    71161 agcgttaaaa cttgcagaca agccttataa aatggaattc aaaggcgtat atgtcatgag
    71221 tgtagagaaa acatcgaatt tctttggcaa gctttcagtt ggagatacag taacaaaagt
    71281 agacgggaaa agctttcaaa gtacagaagc atttatggat tatgtaaaaa gccaaaaagt
    71341 tggacaaaca atcgaaatca gttatttgca taatggtgaa gaaaaaacag cttctggtga
    71401 tctgatcgag ttaccaacag ataaaaaggc aggaataggt atcacgctta ctgaccatac
    71461 cgagatcgaa tcagatacag atgttcgtat taattctggc tctatcggtg ggccatcagc
    71521 gggattgatg ttcactttag aaatctatga acaagtcact ggtaaaaact tgcgacatgg
    71581 taagcaaatt gcaggaactg gaacgatcaa cagtgaagga gaagttggcc gaatcggagg
    71641 aatcgataaa aaagtggcaa gtgcggataa agctggcgta gagattttct ttgcaccaga
    71701 tgatacgatt tcagacgatg ttaagaaaga atatcctgaa ataaaaacca actacgaaga
    71761 agcaaaagct gccgctaaaa aattgaattc ctcaatgaaa atcgtaccag taaaaacagt
    71821 acaagaagcg cttgactatc tagagaaaat gaaatgaaac taaataatta aagaggggtt
    71881 gtgacacgag ttctgtcaca acccctcttt ttcgtcaaaa atcttcacga taaaaataaa
    71941 aacaaaagaa aaaattgacg taattagatg ttgaagggac gattttgcca gagctcactg
    72001 tttcttcaat tttaaatgaa aggagtaatg aatgattttt actggtcatt ctttgaaaaa
    72061 agaacaatga tagaaaaaat cagcaagaaa atactagctt cacccaaatt gatcatttgg
    72121 atgtctatga tagttcttat cttttgtatc gcaagtggtt ttttattatc aaatttgcta
    72181 ttttatagag aaacagaaga gactgctgca aaagaaatac tttcttcgtt tagttccagt
    72241 ggagaagatc catcagctga aggaaacgat gtagaagagg atgctcgtcc aaaggtcatg
    72301 tacaccgata tcaaaggaag tgtgaaggag cctggtatct attccttttc ttcagaagaa
    72361 cgggtctatg atgtactaaa gcgtgcaggt ggattattag aagaagcgga tagcgatcga
    72421 atcaactttt ctgcgaagat agaagatcag caagtcctat atatccctgc tgtaggtgaa
    72481 gagctgccag aacatcttga tcagttggca tcctcggaag gaaaacagcc tacagcagat
    72541 tccgagcctc ccaaaataaa tatcaataca gccactccat cagaacttca gcagattcct
    72601 gggatcggat cagtcaaagc gcaagaaatc attcgattta gagaagaaaa cggatccttc
    72661 cagaaagtgg aagacctgca ggagatttct ggtatcggtg aaaagacagt tgaaaaacta
    72721 aaaaacttcg ttacaattaa atagaatgat tcaaaatcga ctggcacgag caataaatgg
    72781 acgctaatgg atactaagaa taatccaatt ctaagagctt ggcaggacga ggaggtagta
    72841 tttttggata ggaaatagct tgattgttcg caaagaaaca ttaaaaaaac caatgctgaa
    72901 tgtctatctc caaaataaaa tatctgggat tcatattatg aatacggctg tcagcggcaa
    72961 taacagtcaa gctctacgag aacgctttgc caaagatgta cttagctata cagcagataa
    73021 agtatttatt ctcattggga cgaatgatct agcagagcat aaacaacttt ctaaagaaac
    73081 gtaccaaaaa atctgcagtg gatgatagtt cagctgaaaa aagactatag ttctgatcat
    73141 gctttcttta tcacgccacc agcagtcgat gagacaaaac aaaaatatcg gaataacaag
    73201 ttgatcaaag aatatatctc tgttctcaca gaagtagcaa aacgccatga ttgtgaagta
    73261 tcgattttta tacggaattt cttgaacaaa tggattcgaa gaatttttat aatgggctaa
    73321 tggatgacgg acttcatttc ggcagtgaag ggtaggatct tttgtcagac aggatgattc
    73381 gttatattta aagaatattt tgtttaagca atgagtactt tttaatcaac ttgaaaaaac
    73441 ggaggaatat aaaacatgtt agaaaaatct tcaaaagcat gcgatacgaa taaagaaaaa
    73501 gttactgata agtcacataa tgaacgcatt ccttgggacc agtactttat ggctcaggca
    73561 gtactgttat ctttacgtag tacgtgtaca cgtttagaag taggagcgac actcgttcga
    73621 gaaaaaagga tcattgctgg cggctataat ggagcagtca gtggagatgt ccactgcata
    73681 gatgaagggt gttacgtcgt ggatggacac tgcttgcgaa cgatccatgc agagatgaat
    73741 gcactcctac agtgtgcaaa acttggtatc ccgacggaag gttcagaaat ctatgtcaca
    73801 cattttccat gtttggcctg tacaaaggct ttgttgcaag ctggtgtgaa agggatcaat
    73861 tatctccatg actatcgaaa tgatccttat gcccaagcgt tgatcgaaca attaggtgta
    73921 tctgtacacc aagttgcttt agactcacag catttttcaa aacttcaaaa tgaattaaca
    73981 ggaaaacatg tctagcgttt tagaaaggca tgttcgaaat cggttgattt ttccggcaat
    74041 tacgtcgatg attatctgtt tgttgtatta cactgacaat tggggttatc gtttgcttct
    74101 tgccggaatt tttttatggt tttcgctaaa agaaggacgt tttatcgcgt acttgtgtat
    74161 tttgtgtagc ataacggtat ccatttctta taggctacaa gagtattcat ttttagagga
    74221 aaaaacaaca tctgcgcaaa ttactgtatt tacagatacg attcgtgcaa atggtaattt
    74281 tttgacaatg gaaggaaggt tatcagagaa taaacaaaag ctccagcttt cttatcaagt
    74341 aaacacagaa gcagaattga aaacatggct taacttccga ggaaagggaa tagaattgac
    74401 tgctgaaggc gagttcatct ctccagcaaa aaagcggaat ctttataatt ttgaccaaga
    74461 agcttacttc aggaaccata gaatttctgg acggttccaa attcaaaaga ttgaacagat
    74521 caccatgcaa aatcactggc gaaactggct tcagagaaaa agaggatctt ttatctcctt
    74581 tgtccaatct gaatttccgc aaaaaacaag tgtatatatt aattctttat tatggggcta
    74641 taaagatgca ggattcagag aaccagaaga aatttttagg gagagtggtt tgctgcatct
    74701 gttcagtcta tcgggtctac acatccaatt ttatttaggg tggctgtatt atttatttcg
    74761 acgtatcgga tggccactac acgtcagtat tattccgttg agtctactaa ctatctgtta
    74821 tgtttctatt gcaggaagtt ctatttctgt tttgcgtgct agtttgctgt ttttattgcg
    74881 attgctagtc aaattgctga ataagcatta ttccaccatg gacttatttg cagttgtctt
    74941 ttggcttctt ttactatttg atcctcgatt tctttttcat gcgggtggac agcttagttt
    75001 tttcatgagt tttttgttta tcctgatttc ttttgatgga gattggttga aaaaaacggc
    75061 ctctcacgtc ctgtttacta tgatcacgat gccgttgatt gtatggcatt tttatgagtg
    75121 gcctatttta ggtagtcttt ttacattact gattgcaccg gtattcaagt ttctttttct
    75181 tcctggtgtg ttatttctca cactcttcaa tcgctggctg ccggaaactt tacttttgct
    75241 gatagaagag ggattaatct tatttgagcg aataatcgat ttttcttcag ggtcgtctct
    75301 cattatagga cgtttgcccc ttgtgctatc tgggatgtat attttcggat tactgatttg
    75361 tatggatagg ttgaaagaac aacctgtgaa acacttgttt tatgttggtc ttttttcttt
    75421 tatttttttg actttgccat ttttacgctt ttcttcaacg atcgctttta tcgatgtcgg
    75481 tcaaggagac agtatctttt tgcaaagtcc ttttcgaaaa gaaacgattt tgatcgatac
    75541 tggaggtcag ttacactttg aaagagaaaa gtgggcgcaa ggtatgatac gtcctcctgc
    75601 tgaaagaaat atcgttccat atctaaaagg tcaggggata agtgtaatcg ataaactcat
    75661 cttgacacac gacgatacgg atcatgtggg agaattaccc acgttatctc agcattttac
    75721 tatcaaaaaa atttatatcg gttggggcgc tggacgaaat gagccattac aaaggcattt
    75781 atctgaagct caaaaaaatg gtactgagat cattgaaatc aaacatgggg acaggatcag
    75841 cggttattat gatctttatg tattgactcc ttttgaaaag ggagagggaa gaaatgaaga
    75901 ttcgattggt ctatggatgg aatataacaa taggcgcttt ttatttttag gagatttgaa
    75961 tcaagcaatg gaaaaacaat tgcttttgat ttatcctaat ctgaaagccg atgtagtcaa
    76021 attaggtcat cacggtagcc gtacgtcaag taacacagat tttctttccc atattgaagc
    76081 aaaacatggg attatatcct gcggcgtaaa taatcgttat ggtcacccgc acagcgaagt
    76141 attggagaca ctagaagaaa atcagataaa aacatggcga acggatcaac aaggaatgat
    76201 cacctatcgc tggcatccag tttttcatcc tcatgggcta gtggaaacaa tgatagacta
    76261 gtaattctca tctgctcatg gtaggataat ttttgaaagt ggtgagtaat ttgagtatac
    76321 aagcagagtt acaaaaaata cgtaaagaaa aacttgcatc ttgttacttg attttaggaa
    76381 cagaaaagtt tctccaagat caagttcgga cagaaattgt caaaaaaatc caattagagg
    76441 gcgaagatga cttaaatttt ttaagttttg acatggaaaa tgcaagttta gatgaggtag
    76501 tagctgaagc agagacgttg ccgtttttcg gagatcaacg gttagtattc gtagagaatc
    76561 cttatttttt aacaggtgaa aaagtaaaca atggaatcga acaaaatact gatttattga
    76621 cggcttattt gaaagaacca ttagaatcga ctgtattagt attctttgcg ccatatgaaa
    76681 agttggatga acggaaaaaa gtaacgaaac aattgaaaaa aacagcaatt atcatcaatg
    76741 tccaacagtt gaatgaaaaa gaagtacgtc aatatctgat gaatacctta gagaattcct
    76801 caatcaaaat ggaacgaagt gcaattgatt tattcttgag gttgaccgac ttagatttat
    76861 ccaaaatgat gagagagtta cagaaattgg tcctttatgg acaaaatcaa cagcaaatca
    76921 cggtaagaga agtagaagaa ttagttccta aaacgttaga acataatatt tttgacatga
    76981 cccaatatat tttaaaaggg aagacggaac aagctttgcg tttatacgaa gatttagtta
    77041 tgcaagggga agaaacaatc aaaattaatg cgattttgct ttctcaatta cgtttatttt
    77101 tacagacaaa atttcttgta aagattggtt atcagcaggc aaatattgct gaaacattga
    77161 aaatccatcc atatcgtgta aaacttgcca tgcaggaagt aaaaaaattc gatgaacatt
    77221 tgctggtaca gttgtttgat caattagttg agatggatta tcaaatcaaa acgggacaaa
    77281 tcgaaaaaga attaagtttt caactattcg ttttgcgcac agggcagttg ttaagaaatc
    77341 gataagaaga aagacgaggg caaagcatgc gggaaggtcg tttgtttttg tccttttttt
    77401 gacaaataaa aaaatcggcc gaagccgatt cttttattta gcgatttttt tgcttaaacg
    77461 agatttgtcg cggttagctt tgtttttgtg gattagacct tttgattcag ccatatcaat
    77521 agctttaaca gcttctttat ataacgcatc tacgttgtca gcacctgaag ctacagcatc
    77581 ttcaaatttt ttgattgcag tacgcatagc gcttgtttga gatgaatttt tcacattagc
    77641 gttttcgctt gtacgaacac gtttgattgc agattcaatg tttggcatta gtttcacctc
    77701 cgataactta agttcttacg cgtgttaccg cataattcaa cattcattat tatacctaaa
    77761 ggcagcgtgc tttgcaatat gaacatgaca agtttttttc cttaacagtc ttttattagg
    77821 attttttttc agtaaaaacg gatagtacaa gctgaaaaaa atcaaaaaga cgctattgtg
    77881 ggaggcaata gcgtcaaacc aaactagatt tttatttatc tatttaaaca aagtttgaat
    77941 gggaatgttt ggtactaagt tattatataa caaagagaac cgtttgcaaa gttttttttg
    78001 aaaagggata cgattccctg ttgttctttg tttttctatt aagaaactgc agttatttta
    78061 taaccagtat cctttgatca gtaaaggcta ttctggtgta ccgattattt taatgtattc
    78121 atcgatattt tttatcaaca ttttttttat gaccaactgg ttccgacggt tccatttgcc
    78181 acttacttcc agcacatatt tatttagggg caaacgtaag atatattttg ccaaatagcg
    78241 atctttaagc aggccattga tcacacggtc ctcaagctgc aagttgatcc cagtcagcat
    78301 cattggctgt tcttgtaaaa cgtacgtatc aattaaaaga ccttcaaaag tttccacaag
    78361 cgtcttcctt tcttttcaaa gtaacctgaa aacaggcact aattcttcca ttttgatatt
    78421 cgttgaagtg gatgcgatca ttctttcttt tgtccactgt aatctagtat aacaaaaaag
    78481 agcagaagag ccaaaaagaa aaaagagcat cgaattctat aggaagtctc attggcacgg
    78541 cctaaattat tacaaaacat tgattaaaaa aagcccctgc atcgcaggag catcccctat
    78601 atcgttttaa taggtcaatc aaaaagcagt atcaaacaca ttttgtatca aatcaggaaa
    78661 taatagataa gctaagccca gaatcaaaag taccgctaga agaaaccagc cgattgttcc
    78721 tatgaaatac tttaattttt cgaacattgt atcacctcgt tgtcagtata tcacggatca
    78781 gtaaattggg ggtgttaaga atctcctaaa atagacaaaa tttgttctat tttcctaaag
    78841 caaatacttg taagatacag taagtgatac cagaagcaat gaccggtccg gcggcgattc
    78901 ctcttaagaa gacaacacct aaaatcgtgc cgaaaacaag tgcaacagtg atttgtggat
    78961 cagaagcaat caatcctaca cccttggaag ataagacagc cactagccca ccgcaaaaaa
    79021 cagctaccca gccaataggg gatttaaagg cttctaatag atcacgtaga ccgatttctc
    79081 ctgttgcgat agggattaga atagccgctg tgatgatcgt caccccaata ttgattcctt
    79141 tgccttgaag ccaagggaaa aaggaacggg aggctggtat gagcttcata atcaaaacaa
    79201 aagctgccgc tatcatcaga ctttgatttt tagctaaaat tgctacaagt aaaataattc
    79261 ctaaaaaaag ccaactttcc atgaaatata ttcctcactt tcttcgaaac aatcataaca
    79321 tgtttttaga aagttacaaa agtaggaatt catccttcaa gacctattca taagctgttt
    79381 ttttcataaa ataaacaaaa tcataaaaaa acactttgct tatttgacag aagacagccg
    79441 cgctttgtat agtggacaag acttacattt ttttgaagaa tcagaaagat caggtaaagg
    79501 tcttagtaaa cgcaagccat tcagaataac caatattgtg cttccttcgt gtcctacaac
    79561 tccaagaggt agatttaaaa attgcaggaa gttagaagca atcaacaaga gaataacaag
    79621 aatcgaaaaa ataatatttt gtttgatgat ccgccgcatt tttaaagata gccgatgact
    79681 gtagacaaat ttatttaagt cgcttttcat taagacgatg tctgcgatat ccatagcggc
    79741 atctgtccct tgccccatag cgacacctaa agaagcggct gctaaagcag gcgcatcatt
    79801 gactccatca ccaaccatcg catttacttg gtaaagttca ttctcttttt tgactagttg
    79861 tgttttttct tccggagtac agcttgcata gtaatgttca atccctactt gttcagcaat
    79921 ggcctttgct gtgccagaat ggtcgccggt tatcatactt gtatggatgt tttgggattt
    79981 aaagtaggaa attacttgtt gggtatttgc ttttggaata tctagcagac ctaaaacagc
    80041 aattaactgc tgatctttag aaagataaat cacggttttg ccttgatttt ccaaacgttc
    80101 gatcatttcc gcaatctctt tgctgattat catcgtctca ggatcatatg cgtgcttgcc
    80161 aactttccat tgcgcaccta aataggtagt ttgtaatcca aaaccagtaa tttcttctat
    80221 tggtaagtat tggatttctt caggtattgt gcaatcgaaa tattgcaaaa tcgcatgagc
    80281 aagtggatgc gtcgtttttt gttccatagc aacaagaagt tgttgtgcca aatccttttc
    80341 atcaaagaaa aagctgtcag ttacaactgg ttttccttta gtgagcgttc ctgttttatc
    80401 aaaggcaatc gcttttaatt cagaaagctt ttctaaatgg ataccgcctt tgattaaaat
    80461 cccatttttt gcagaatttg aaagagcagc tagagaagct ggtgtagcag atgcgacaag
    80521 cgcacaagga gaagccacaa ctaataatac cattcctcga taaaagcttt catcccaact
    80581 ccaacctagg aaaaagtggg ggagaaggat cattagagga acagcaagca aaatcaattt
    80641 tacataatta ttttcgattc gttcgataaa tgaagctgtt tgagttggcg tattctgcgc
    80701 ttcttctact aattggataa tcttagcaaa gacagtttcg gaactcgttt ttgttactcg
    80761 aatcgtgatg ggatttcctt gattcagcgt tcctgcatac atctcatcgc ctaaatattt
    80821 ttctacaggc acagattcac cgcttaatgc agactcatcg atcgttgcct gtgtgctttc
    80881 taaaaagcca tctatcggaa cagctgctcc tttagcgaca aataccaagt tatccacctt
    80941 tagttgagaa acatctactt cttgcatttg tccattttct tttaataatt gggctttttg
    81001 tggttgaaga ttcatcaaag cagtaatttc tttttgactt ttattagttg tgtattcctc
    81061 taatgcaccg cttaaacaaa agatgaatgt caacattgct ccttcaaagt aattaccgat
    81121 caaacaggca ccaatagcag ctaaagccat caataaatcc acattcagtg tccgatcatt
    81181 ccacaattct ttcaagcctt ccgatgtttg tttatatcca ccgatgataa tggcactgat
    81241 ataaaaaagt ggggtgatgg taagagagaa tcggtcgaag ataaaaccga agatcataaa
    81301 taaaaggcag ccgattgttg ctgcaaaagc ttttttctga ttagtcatcg ttattagacc
    81361 tccttgtcaa aacaaggata gcataataaa aatgtaattg ataataataa cagctaaatg
    81421 agaacgataa tggttatcat ttggtgatta agcttgatgt tgtttttatt atttataatg
    81481 attattatta aagaaaataa aaaaccagaa catttctcaa ataagagaag tgttctggtt
    81541 ttttctacat tgcaataaac caataaccaa tcaaaatgat accaaggata atacgatacc
    81601 agccaaaagc ggtaaagtcg tttttcttca aatagcttag taagaatttg atagctaaga
    81661 cggaaaccac aaaagatacg aatgttccta caagaagaac aacagtttct tccattttga
    81721 atgaattccc ttcagctaaa tatttgacga tcttcaagcc gcttgcgcca aacatgacag
    81781 gaatccctaa gaaaaatgaa aactctgtag cgacaaaacg cgaagcacca atcaagatag
    81841 atcctaggat cgttgcgcca gaacgggatg ttccgggaac aagtgccaag acttggaata
    81901 acccaacaag aagagctgct tggtacgtaa agtcattcag agaagtccaa cgaggtgttt
    81961 tgtttttgtt gcgtttttcg acaataataa aggcaatacc gtaaacgatc aaaacaatgg
    82021 caactggtaa gaacttgtgg aaatttgctt ctaaccaatc atccaaaggc aaaccaatga
    82081 tgatggctgg tatgcaagca acgatcactt taaaccaaag tgtccacgta tctttttttt
    82141 ctaccgttga tttacttgga gaaaatgggt ttaacttgtg aaaataaaga acgacgacag
    82201 ccaagatcgc ccctaattgg ataacgacat tgaacatcga cataaattgt gaactggcat
    82261 ccagtttgat gaattcatct gctaggatca aatgaccagt actactgatg ggcagccatt
    82321 ccgttattcc ttcaataata ccgagaatca ctgctttgat aatatttgca aaaaacataa
    82381 attccttcct tcccaattaa gatacatctt atagtatacc gttatttttt gagaaaccca
    82441 atctgttttt taatattaag aaaaacttga gaaataaagc agataagtac acgaagagaa
    82501 aaaagaagac aaagccctcg ttcagacttt gccttttatg attctctggt aaaaattaaa
    82561 atatccccat tatagaaacg agtcgcgact atgtgttttc ccgttatcga tataagacgt
    82621 atcattgatg ccgtggatag aaaatcgacc gtttgcataa gtgacctttg ttacgctgcc
    82681 atttaaaagc ccagcacgaa caggctgttt aggatcgatc agattaagca aaaaggcgat
    82741 cgtcaaacca tgagatacga ctaaaacatt tccaccacca tttttttcgc agtgatgggc
    82801 gatatcttca aatcctgtcc atacacggtc tcgcaatgtt tcataagttt ccgcccaccc
    82861 agcggtatct aagttgtata cagcattagc aatttgttca aaagaaactt ctgtggtaaa
    82921 catttcatca tatgttttaa aattcaatac acgagggatc acaccccaca tttccatgtc
    82981 atatccacct tcaagagacc cgaaacacca ttctctaatc cttttttcaa taaaataagg
    83041 aatttctttt cccttagaat gttctcctag aataatacgg gcagtttcga ttgcacgacc
    83101 actgtcacta gaataagcaa atgaaaagtc gacatttttt aacccgcgac ctaaataatg
    83161 aatgccttct tgtccttgct ttgtgagggg tgtatcacac cagccttgta ctcgttcaat
    83221 tgtattgaac atcgtttttc cgtgtcgtac gatataaagt gtagtttctg tcattttatt
    83281 ctcctcctaa gtcgttttaa cgattgaaaa aaggatttgt ccttttttcc tgttcaatcg
    83341 tcgttgcatc gccatgtcct ggatagactg caaattcatc aggtaataca aacagctggg
    83401 tccgaatact atgaagtaat tgttctaaat tccctgtata cagatcggtt ctaccgatac
    83461 ttcctctaaa cagggcgtcg ccagaaacga caaaatcatc aaaaataaag ctgacgcttc
    83521 ctatagaatg ccctggggta gggacgacgc gaaaagacat gtttcctaaa gtataagttt
    83581 tcatttcaaa ttcatattct gcaggcgaga caataacatc agcaatatca tcatgtctac
    83641 cgagtccaga aagattcaaa atcggatcac ctagccattt ttgctctagt gggctgacat
    83701 aaactgggat ttgataatac gcgcgtaaag gttccactgc accgatatga tcgtagtgag
    83761 tatgggtaag caaaatcgct accggctgtt ggtttgtttt tttgatttct tgtttgattc
    83821 gttccgcatc ttctccaggg tcaatgatca aaagtgcttc ttcgttgtag accaaataac
    83881 agttttcttc aatcattcct gtttttaatc tttcgatatg catgtgcatt cctccttgat
    83941 agccaaaact tctttcatcc attagtataa cgcattcatt gctaggaaac aaaaacaggt
    84001 acttatttct ctttacttat ctgaattatc agactatatg aaaagtattt ggatattcta
    84061 aagagaagga aagtaagttt tcatagcatt ttaatatgtc cttgttacat tagattcatc
    84121 caaaaatcca aattctatat cctatatccc tgcctaatca gcagggtgtt tttttatgtt
    84181 tggaaagaga gctctttttg ctaaactagg ttttttcagg taatcttaga gagaaagagg
    84241 tggtggaaat gatcaaacag tcaaaaatat ctgtgcggca cttagtagaa tttattttgc
    84301 gacgtggaag tatcgacaat cgtaaaaaaa gtaaccatac tgcattagaa ggagcgagaa
    84361 tccaccgcaa gctccaaaaa gatgcgggag aaaactatga aaaagaagtc tttttaaaaa
    84421 caacagttca attagatgaa tattacctga tcgttgaagg ccgtgcagac ggtattttca
    84481 aaagagacgg tatttattat atagatgaaa tcaaaacctc cgaaccacga tttgaagatt
    84541 tagaaccaga acaagtggaa cttttctttc atcaagcaag ggtgtatgcc tatatctata
    84601 gtcaagaaca tgatttacaa gaaataaatc tgcaattgac ttacttccag acggcagaag
    84661 aagtgatcac gagaaaggtt gaacatcaga caagagagca gttagaaact ttttttaaaa
    84721 agctgactga agattataaa gaatggcttg tttttcaaga aaactggcgc actgtacgca
    84781 atgcctcttt gatggctttg aaattccccc atgatgagta taggaaaggt cagcgtgaac
    84841 tagcggtagc tgcttataaa acgatccgta cgaaacagaa attgtttgta gaagcaccta
    84901 ccggaacagg aaaaacaatc tctacgttat tccccgcttt gaaagctgta ggggaagaag
    84961 agggagaaaa gatcttttat ctaacggcta aaacaataac tagacaagta gctgaagatg
    85021 ctatgacagc attaaaagat acaggagctg aagtaaaaag tgtcactttg acggcgaaag
    85081 acaagatctg ctttttagat gagacgacct gtaatccaga tcagtgccca tatgccaatg
    85141 gttattacaa tcggattaat gaaggtttat gggatctttt gaatcatgag aatcagatca
    85201 ctcgtgaagt catcgaaact tatgcaagaa agcatacact ttgtcctttc gaactatcat
    85261 tagatgtcag tatctggtgt gacgtaatca ttggggatta caattattta tttgatccaa
    85321 ctgtttattt aagacgtttt tttgaagatg aaaaaaacga ggactatctg tttttgattg
    85381 acgaagcaca taatttggtg aatcgttccc gagagatgta ttcggcagaa ctatcttatg
    85441 aaaaaacgaa acgaagtaag gaagctatac caaaggaatt caaaaaactt catcgccgtt
    85501 tcaacaaact attaaaagaa tttgattcga tccgagaaat agcaaaagaa gatcattggg
    85561 attatcatca tcaaaaagca ccggctgaat ctttagtaaa ggcaggttat caattaagcg
    85621 aaaaaattaa agaatggctg gcagaatttc ctgagcaccc atcacaagaa caattgcttc
    85681 cttattattt tgatttgctt cattttttga aggtcagtga attttatgat gatcactacg
    85741 aaacaacagt cgaaaaaacg tatcgcgatc tgatagtaaa agagttttgt attgatccaa
    85801 gcttgtttct tgagcagtcg ttggataaag gaaaaagcag tattcttttt tctgctagtt
    85861 tttcaccact ctcttattat caggaaacac tggggggaca aaaaagtctg gcttacaaac
    85921 tgccgagtcc tttcccagaa ggaaaccagc aagttttcat cgcaaattac ctagagacga
    85981 cttatcgaaa aagggaacac agtctggcaa aattagtaga gacgattcat gcttttgctg
    86041 atggaaaggt cgggaattat ttcgttttct ttccttctta tcaatattta gatcttgcgg
    86101 tggaagcatt cagacagcaa tatcccgaca gcaatgtgct aatccaagaa acggacatga
    86161 atgaggaaga acgcgaacga tttttaacaa aatttcaaac aaatcccgct gaaacattga
    86221 ttggattttg cgtacttggc gggatttttt cagaaggaat cgatttgaga ggggaccgat
    86281 tgatcggcag tatgatcgta ggtgtaggtt tgccgcagat gaatcatgag caggaactga
    86341 tcaaatcata ttatgatgag aaggaacacc ttggatttgc ttacgcttat cagcttcctg
    86401 gaatgaacaa agtcttgcaa gctggtggtc gagtgattcg tggcatggcg gatcaaggaa
    86461 tcgttgtatt ggcagatcaa cgattttcta gtttttctta tcgacgttta ttcccacaac
    86521 attggcaaac agcaagagaa gtacgaagtg ttggggaatt ggaagaagga atacagcgtt
    86581 tttggttatc aaaagaagca gtaaatcaaa aaaacgagga atgacagacg tcagcctcgt
    86641 tttttgatta acgatagtta gtgaattgca actcgattgg taagtcaagt ttacgtaata
    86701 aagcaatcac agcttgtaga tcatcacggt tcttacctgt cactctaatc tgatcttctt
    86761 ggatttgtgc ttttactttc agtttttcat ttttgatagc tgtagtgatt ttcttcgcgt
    86821 tttcacgatc gattccgttg accaaatcgg cagtttgtcg agctttgcca cctagagcgt
    86881 gttctgattc ggaaaaatgg atatttttga ttggcacatt tcttttgatc agcttaccaa
    86941 ataatacatc tttgatctgt tcgattttga aatcatcatc gctcaataca actagattgc
    87001 tgttctctaa tttgatttct actgtagaat ccttgaagtc aaagcgattg gacaattctt
    87061 ttgttgtttg ctgaatcgca ttttttactt cttctgtatt gacttctgat acaatgtcga
    87121 agctagcatc ttttgccata ggaaccctcc tgatatacat ataaatattt accatacatc
    87181 tatcttatca taggattagg gcagattctt acctgtttgc taaaattctt ctcatttgag
    87241 ttcgaagtat tttattttaa tagataaact gttattctta catttggaat accaaagtag
    87301 gggagaggtg cttgtgagta aaattatgac tgcgtatcat aaaaatgaaa gattaaagaa
    87361 aatagtggtt attttgataa ccggattgac cgctgcggtc gctttaaatt attttttgat
    87421 tccagccaat gtcttttctg cagggatgaa tgggatcgct caaatcattt cctcgctttt
    87481 atatgaatgg tttcatatca agatagacac aggaacattt atttttcttt tgaatatccc
    87541 tgtgtttgta ttagggtttc tcaaagtagg aaaacgagca acgatcctta gttttgtgaa
    87601 tgttgtatgt gtatcggtga tgacgatgct gcttccagtc ggacagatca ctgataatat
    87661 tttgatgaat gccctagtag gcggtgtttt attaggtctt ggcgtaggat tatctttaaa
    87721 aatgggcttt acaactggtg ggatggatat catctctctt gtactttcac aaacaactgg
    87781 aaaaacagtc ggaaactaca tgctgattct aaatggtatc attgtagtca tcgcaggctt
    87841 cttctttaac tgggaaagtg ccttgtatac gattatttcc atttatgcaa tgactcaagt
    87901 aattgatgcc atccacacga gtcatcaaaa agtaacagca atgatcgtta ccgtcaagcc
    87961 agaagaggta acagaagaac tagcaaaacg gatggtacgt ggaatgacat tactgccgtc
    88021 tgtcggtggc tattcaaaaa aacaagggaa catgatcatg atggtcatca cccgctacga
    88081 aatgtatgat cttgatcaga tcgttcatga aatcgacgac gatgcattta tcaatatttt
    88141 accaactcat tcagtatttg gccggtttgc aaatgaaaac gaacaaaaaa tgtatcgaac
    88201 aactggcgta ttgccagaaa taaaaaatcg ttcacaaaaa aaatgaggaa tcaggtttat
    88261 tttagaataa atttaatcta ttacatctaa taagaacatt tttataaatt taaacgagaa
    88321 tcttacttct aaattctcat ttacaagcag ctaaaaaaag agtatgataa ataaaaatcg
    88381 tgttaaggag tgtcgtacga tgagcgaagc aaaccaagaa cggtctggac aagaaataga
    88441 actaatcaaa gaacgtattc ttgctgcctt ggaaatggtg attgatcctg aattaggaat
    88501 cgacatcgtc aatttaggct tgatttatga tatagaattc aatcctgaaa atggagaaac
    88561 agtcattaag atgactctaa caactatggg ctgtccatta gcagatattt taacagaatc
    88621 tatccatgac gcattaaaag aagtgccaga agtaacgaaa gcagaagtca aactagtctg
    88681 gtatccagca tggacgacag ataagatgag ccgttatgca cggattgcgt tggggatacg
    88741 atagaaatct tggctgacta gctatgagaa gcaaatagga aaaacaaaaa agccttttat
    88801 cctttctaga agaaaagaga taaaaggttt ctttgtttat aaaattttgt ctgctatatt
    88861 ataagttaat taaataaatg tgttctattg ataaaggaca ttcaaatccc tttccttttg
    88921 gaatccttct tttttacatc agtttgattt tcgtatagta ttctttctca tcttcacgat
    88981 aaatcgcagc cccatttttt tgttgtgctt taagactgcc aagatttttt ttatttacag
    89041 acaaaagtgc ctcatcaatg ccgatttttt ttgcctcttg tagtactaat gctaatcctt
    89101 gtgtggcata tcctttttta cgataattag gggagatgcc gtaaccaata tggccagcgc
    89161 cgtttcttaa tgtttcgttc agtttatggc gcaaattaaa aattcctaca tactcatgtt
    89221 catcactttc taaaataaag acggttgcgg gaacgtaact ctcaggaaga tcgattccta
    89281 aagacgtttt ttcgcatttt tttacataat ctaaaaattc agcaaatgta tagccataag
    89341 cattgttgat aaatccactc tcatttggtc caaagctagt ttgtagctga taagctttct
    89401 ttaaatctgc ttcccatagt ttgatcagtt tcatcgtatt cacctcttta agtaacctta
    89461 ttcatttgtt ttctactata gcactattgt tgtaaatagc catgcctatt tttatttatt
    89521 gtgtcatatc gtattaagaa tcatcgaaca atacaaatat gaattacaat aaaaaaatta
    89581 tttgaagttc gttcattcgt tacttatgat ctctagaaga aagaaaacgt gaattacttc
    89641 agcgtctaga attccagaag gatagtaatc aacagaagaa ataatcaata tccaaatgaa
    89701 agaagatagc tgaatcgaga aaaagaggaa aaaagaaatg aagaaaaaaa taaaatagtt
    89761 actaatccca gagtcgttcg tttaacattg aaatcgatca tatggataaa acagattttt
    89821 tcaccaaccc tcaatagatg atggagttgc tcggatgaat tatttgtgta gatggcataa
    89881 gggagggcgt acagaaatga aaaagaacaa aaatatgttc ttgacaaaaa acacatgtaa
    89941 tcctaaacta aaagatgtga tgcggctatg gcggaattgg cagacgcgct agcttcaggc
    90001 gctagtgagg gtgacctcgt ggaagttcga gtcttcttag ccgcataatt tgttttttta
    90061 taaagagaag gtacacctgg agcgatgaga aagaaaataa catattatct aaaagaatat
    90121 aggaaaaatt ttaaacaacg gaaccagatt caaataaatc aaaaaaaatt tgtgtgaagg
    90181 tgggacagtt taaaacctaa tagattgatt caaatagtag atttatagat tctgatacaa
    90241 gaaatactgg caaacgatag ttaaggaata aagactaaac ttaaactcaa aaagaggtca
    90301 tgcgaaagaa taaatcgcat gacctcttac tatattttga atgtgtttaa acgttcaaat
    90361 aatgaatctt agtaccaacc gtttgcttcc cagaatgctt tcgccgcttc ccaagaacca
    90421 taacgtgaag taacatattg ttctgctact ctttcttggt ttgcagcaga gtagtcacca
    90481 tttaagtaag aagaatctaa ttggtaacgg ccgatgtaac gtccgttcgt tgctgtgtaa
    90541 gaaccgcttg attctttttg tgcgatccat tcttttgcag agcttgtact tgctggagta
    90601 gcagcagttt ctgttacagc aggagcaggt tgtgtttcag caacaggcgc tgcttgtgtt
    90661 tccgtttgaa ccgtttcttg tacaacaggt tgttcagctt gaacaggttc agcttggact
    90721 ggtgcttctt gaacggctgc ttgaggttca gcagctgctt gtgcagagcc ttctgttggg
    90781 attgttaatt gttgtcctga gta
//
LOCUS       DAHUVV010000003        77607 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.003, whole genome shotgun sequence.
ACCESSION   DAHUVV010000003 DAHUVV010000000
VERSION     DAHUVV010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.003"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(107..1594)
                     /locus_tag="NK767_000182"
     CDS             complement(107..1594)
                     /locus_tag="NK767_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693205.1"
                     /translation="MQVKKKIILSILLAIIAGLYSINYFRNVHTISTSGDIAFHFARV
                     KGLSSIFSGPINFTTFNHYGSGVNYFYPYLTFFPAVIFYWISNNLIVSYILYVWLLNV
                     CTIMLMFHYGLKFLKRIDAAFIFSCLYTFYGYRTIDIYHRSAIAEAIALTVIPIVMYY
                     AYALIYEKKPCAIPLAVSMSLLIYTHVLSTFMSVIAVGLLVLGGFVVSETRKKESIRL
                     IVELSKAVGMTILLTAYFWYPMFRQIMVQSINEPDKTTLQHQALNVSDSLIGAMNNDL
                     TTFTVGIIGIISLICPLFFFKGFNRKEKVIYLSAVVSWLLSTKFFPWSLLQNTPIQMI
                     QFPWRILGFQVLFGSLILSFVFMKSTSAKQRKVVNITAIVLLLLAVSAATKANYEHKV
                     VTQRDHLVMNQENLIRYTTLLPGGLYDYAPTEALQYKEHLQNHEVRIGKEWHTMPYQV
                     MDSKIVYTVHESKGQKITLPVFDYWGTVAKVNGQKREIDNRTSVD"
     gene            complement(1917..2396)
                     /gene="rlmH"
                     /locus_tag="NK767_000183"
     CDS             complement(1917..2396)
                     /gene="rlmH"
                     /locus_tag="NK767_000183"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCH6693206.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2824..4113
                     /locus_tag="NK767_000184"
     CDS             2824..4113
                     /locus_tag="NK767_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCH6693207.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4152..4757)
                     /locus_tag="NK767_000185"
     CDS             complement(4152..4757)
                     /locus_tag="NK767_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCH6693208.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(4962..5426)
                     /locus_tag="NK767_000186"
     CDS             complement(4962..5426)
                     /locus_tag="NK767_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCH6693209.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5448..5768)
                     /locus_tag="NK767_000187"
     CDS             complement(5448..5768)
                     /locus_tag="NK767_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCH6693210.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(5912..6991)
                     /gene="pepA"
                     /locus_tag="NK767_000188"
     CDS             complement(5912..6991)
                     /gene="pepA"
                     /locus_tag="NK767_000188"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HCH6693211.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7191..7541
                     /locus_tag="NK767_000189"
     CDS             7191..7541
                     /locus_tag="NK767_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCH6693212.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7764..8711
                     /locus_tag="NK767_000190"
     CDS             7764..8711
                     /locus_tag="NK767_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCH6693213.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8768..10186)
                     /gene="pepV"
                     /locus_tag="NK767_000191"
     CDS             complement(8768..10186)
                     /gene="pepV"
                     /locus_tag="NK767_000191"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HCH6693214.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10335..11180
                     /locus_tag="NK767_000192"
     CDS             10335..11180
                     /locus_tag="NK767_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCH6693215.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11184..11624
                     /locus_tag="NK767_000193"
     CDS             11184..11624
                     /locus_tag="NK767_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCH6693216.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11674..11868
                     /locus_tag="NK767_000194"
     CDS             11674..11868
                     /locus_tag="NK767_000194"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693217.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12004..12690
                     /locus_tag="NK767_000195"
     CDS             12004..12690
                     /locus_tag="NK767_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCH6693218.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13098..13787)
                     /locus_tag="NK767_000196"
     CDS             complement(13098..13787)
                     /locus_tag="NK767_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693219.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13762..15891)
                     /locus_tag="NK767_000197"
     CDS             complement(13762..15891)
                     /locus_tag="NK767_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HCH6693220.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(15939..17276)
                     /locus_tag="NK767_000198"
     CDS             complement(15939..17276)
                     /locus_tag="NK767_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HCH6693221.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17528..17887)
                     /gene="rplT"
                     /locus_tag="NK767_000199"
     CDS             complement(17528..17887)
                     /gene="rplT"
                     /locus_tag="NK767_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCH6693222.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(17940..18140)
                     /gene="rpmI"
                     /locus_tag="NK767_000200"
     CDS             complement(17940..18140)
                     /gene="rpmI"
                     /locus_tag="NK767_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCH6693223.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18212..18736)
                     /gene="infC"
                     /locus_tag="NK767_000201"
     CDS             complement(18212..18736)
                     /gene="infC"
                     /locus_tag="NK767_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCH6693224.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(18780..18905)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19056..19688
                     /locus_tag="NK767_000202"
     CDS             19056..19688
                     /locus_tag="NK767_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCH6693225.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19690..20595)
                     /locus_tag="NK767_000203"
     CDS             complement(19690..20595)
                     /locus_tag="NK767_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCH6693226.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20680..21339)
                     /locus_tag="NK767_000204"
     CDS             complement(20680..21339)
                     /locus_tag="NK767_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCH6693227.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            21655..22599
                     /gene="mvk"
                     /locus_tag="NK767_000205"
     CDS             21655..22599
                     /gene="mvk"
                     /locus_tag="NK767_000205"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCH6693228.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22592..23569
                     /gene="mvaD"
                     /locus_tag="NK767_000206"
     CDS             22592..23569
                     /gene="mvaD"
                     /locus_tag="NK767_000206"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCH6693229.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23582..24667
                     /locus_tag="NK767_000207"
     CDS             23582..24667
                     /locus_tag="NK767_000207"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCH6693230.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24664..25728
                     /locus_tag="NK767_000208"
     CDS             24664..25728
                     /locus_tag="NK767_000208"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCH6693231.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(25977..26780)
                     /locus_tag="NK767_000209"
     CDS             complement(25977..26780)
                     /locus_tag="NK767_000209"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCH6693232.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(26800..27642)
                     /gene="kduI"
                     /locus_tag="NK767_000210"
     CDS             complement(26800..27642)
                     /gene="kduI"
                     /locus_tag="NK767_000210"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCH6693233.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27658..28665)
                     /locus_tag="NK767_000211"
     CDS             complement(27658..28665)
                     /locus_tag="NK767_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCH6693234.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28672..29316)
                     /locus_tag="NK767_000212"
     CDS             complement(28672..29316)
                     /locus_tag="NK767_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCH6693235.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29416..30174)
                     /locus_tag="NK767_000213"
     CDS             complement(29416..30174)
                     /locus_tag="NK767_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCH6693236.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30301..31452)
                     /gene="fucO"
                     /locus_tag="NK767_000214"
     CDS             complement(30301..31452)
                     /gene="fucO"
                     /locus_tag="NK767_000214"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCH6693237.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31534..31845)
                     /gene="rhaM"
                     /locus_tag="NK767_000215"
     CDS             complement(31534..31845)
                     /gene="rhaM"
                     /locus_tag="NK767_000215"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCH6693238.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(31847..32692)
                     /gene="rhaD"
                     /locus_tag="NK767_000216"
     CDS             complement(31847..32692)
                     /gene="rhaD"
                     /locus_tag="NK767_000216"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCH6693239.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32706..33962)
                     /gene="rhaA"
                     /locus_tag="NK767_000217"
     CDS             complement(32706..33962)
                     /gene="rhaA"
                     /locus_tag="NK767_000217"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCH6693240.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(33959..35419)
                     /gene="rhaB"
                     /locus_tag="NK767_000218"
     CDS             complement(33959..35419)
                     /gene="rhaB"
                     /locus_tag="NK767_000218"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCH6693241.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35572..36441
                     /locus_tag="NK767_000219"
     CDS             35572..36441
                     /locus_tag="NK767_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HCH6693242.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36567..37466)
                     /locus_tag="NK767_000220"
     CDS             complement(36567..37466)
                     /locus_tag="NK767_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6693243.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(37631..38455)
                     /locus_tag="NK767_000221"
     CDS             complement(37631..38455)
                     /locus_tag="NK767_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCH6693244.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38466..39761)
                     /locus_tag="NK767_000222"
     CDS             complement(38466..39761)
                     /locus_tag="NK767_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCH6693245.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(39777..40256)
                     /locus_tag="NK767_000223"
     CDS             complement(39777..40256)
                     /locus_tag="NK767_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCH6693246.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40262..41290)
                     /locus_tag="NK767_000224"
     CDS             complement(40262..41290)
                     /locus_tag="NK767_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCH6693247.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41307..42413)
                     /locus_tag="NK767_000225"
     CDS             complement(41307..42413)
                     /locus_tag="NK767_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCH6693248.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(42642..45239)
                     /gene="adhE"
                     /locus_tag="NK767_000226"
                     /gene_synonym="adhC"
     CDS             complement(42642..45239)
                     /gene="adhE"
                     /locus_tag="NK767_000226"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCH6693249.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(45544..45924)
                     /gene="yajC"
                     /locus_tag="NK767_000227"
     CDS             complement(45544..45924)
                     /gene="yajC"
                     /locus_tag="NK767_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCH6693250.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(46021..47166)
                     /gene="tgt"
                     /locus_tag="NK767_000228"
     CDS             complement(46021..47166)
                     /gene="tgt"
                     /locus_tag="NK767_000228"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCH6693251.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            47384..48082
                     /locus_tag="NK767_000229"
     CDS             47384..48082
                     /locus_tag="NK767_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCH6693252.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(48250..50889)
                     /locus_tag="NK767_000230"
     CDS             complement(48250..50889)
                     /locus_tag="NK767_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCH6693253.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(51508..52539)
                     /gene="queA"
                     /locus_tag="NK767_000231"
     CDS             complement(51508..52539)
                     /gene="queA"
                     /locus_tag="NK767_000231"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCH6693254.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            52732..53907
                     /locus_tag="NK767_000232"
     CDS             52732..53907
                     /locus_tag="NK767_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HCH6693255.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            53911..54552
                     /locus_tag="NK767_000233"
     CDS             53911..54552
                     /locus_tag="NK767_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6693256.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            54549..55466
                     /locus_tag="NK767_000234"
     CDS             54549..55466
                     /locus_tag="NK767_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCH6693257.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            55463..56134
                     /locus_tag="NK767_000235"
     CDS             55463..56134
                     /locus_tag="NK767_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6693258.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(56675..57163)
                     /locus_tag="NK767_000236"
     CDS             complement(56675..57163)
                     /locus_tag="NK767_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HCH6693259.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(57364..57630)
                     /locus_tag="NK767_000237"
     CDS             complement(57364..57630)
                     /locus_tag="NK767_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCH6693260.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(57630..57893)
                     /locus_tag="NK767_000238"
     CDS             complement(57630..57893)
                     /locus_tag="NK767_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCH6693261.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            58369..58554
                     /locus_tag="NK767_000239"
     CDS             58369..58554
                     /locus_tag="NK767_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCH6693262.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(58696..60519)
                     /locus_tag="NK767_000240"
     CDS             complement(58696..60519)
                     /locus_tag="NK767_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCH6693263.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(61117..63822)
                     /locus_tag="NK767_000241"
     CDS             complement(61117..63822)
                     /locus_tag="NK767_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCH6693264.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(64128..65075)
                     /locus_tag="NK767_000242"
     CDS             complement(64128..65075)
                     /locus_tag="NK767_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCH6693265.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLEASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            65265..65534
                     /gene="rpsN"
                     /locus_tag="NK767_000243"
     CDS             65265..65534
                     /gene="rpsN"
                     /locus_tag="NK767_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCH6693266.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(65710..66072)
                     /locus_tag="NK767_000244"
     CDS             complement(65710..66072)
                     /locus_tag="NK767_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCH6693267.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(66083..67204)
                     /locus_tag="NK767_000245"
     CDS             complement(66083..67204)
                     /locus_tag="NK767_000245"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCH6693268.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(67220..67570)
                     /locus_tag="NK767_000246"
     CDS             complement(67220..67570)
                     /locus_tag="NK767_000246"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCH6693269.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(67700..69211)
                     /locus_tag="NK767_000247"
     CDS             complement(67700..69211)
                     /locus_tag="NK767_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCH6693270.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(69533..70885)
                     /locus_tag="NK767_000248"
     CDS             complement(69533..70885)
                     /locus_tag="NK767_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCH6693271.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(71038..72114)
                     /locus_tag="NK767_000249"
     CDS             complement(71038..72114)
                     /locus_tag="NK767_000249"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCH6693272.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            72310..74055
                     /locus_tag="NK767_000250"
     CDS             72310..74055
                     /locus_tag="NK767_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HCH6693273.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(74335..74772)
                     /locus_tag="NK767_000251"
     CDS             complement(74335..74772)
                     /locus_tag="NK767_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCH6693274.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(74756..76129)
                     /locus_tag="NK767_000252"
     CDS             complement(74756..76129)
                     /locus_tag="NK767_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693275.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(76451..76939)
                     /locus_tag="NK767_000253"
     CDS             complement(76451..76939)
                     /locus_tag="NK767_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6693276.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(76961..77563)
                     /locus_tag="NK767_000254"
     CDS             complement(76961..77563)
                     /locus_tag="NK767_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCH6693277.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        22344 a        16542 c        14189 g        24532 t
ORIGIN      
        1 actaattaaa agaaagaggt aactacataa actaattaaa aaagctaact tcatagttgg
       61 aggataaatg gaagaaactt caatgattgt ttctccttgt gttccttcaa tcgacactag
      121 tcctattatc aatctctctt ttttgtccat ttacttttgc gactgttccc caataatcaa
      181 atacaggaag cgttattttt tgtcctttag attcatgtac tgtatagact attttcgaat
      241 ccatcacttg ataaggcata gtatgccatt ccttgccaat tcttacttca tgattctgca
      301 aatgttcttt gtattgtaat gcttccgtgg gtgcatagtc ataaagtcct ccgggaagga
      361 gtgtcgtgta acgaataaga ttttcttgat tcattaccaa gtgatctctc tgagtgacaa
      421 ctttatgttc atagtttgct tttgttgcag cagatacagc caacagtaat aaaacaattg
      481 ccgttatatt aactactttt ctttgcttag ctgatgtgct cttcatgaaa acgaacgata
      541 aaatcaacga gccaaacagt acttgaaagc ctaagattcg ccaaggaaat tgaatcattt
      601 gaatgggtgt attttgtaat aaagaccatg gaaaaaattt ggtggataag agccaagaga
      661 ctaccgcact aaggtaaatc actttttctt tgcgattaaa tcctttaaaa aagaagagtg
      721 gacagatcaa agaaataata ccaatgattc ctaccgtaaa ggttgtcagg tcattattca
      781 tcgcaccaat caaactatcc gagacattta atgcttggtg ttgtaaggtc gttttatctg
      841 gctcattaat cgattgaacc atgatttgtc ggaacatcgg ataccaaaag tatgctgtta
      901 aaagaatcgt cattcctact gcctttgaca actccacaat caaccggatg ctttcttttt
      961 ttcttgtttc acttactaca aagccgccta agactagtaa gccaacggct attacactca
     1021 taaaagtgga aagaacatgt gtataaatga gtaagctcat actgactgct aatggaattg
     1081 cacatggctt tttctcataa atcagtgcgt aagcataata cataactatc ggtataaccg
     1141 taagtgcaat cgcttcagca atagcagaac gatgataaat atcaatcgta cggtacccat
     1201 aaaatgtata taaacagcta aatataaaag ccgcatcgat tctttttaaa aattttagtc
     1261 cataatgaaa catcagcatg atagtacaaa cattcaatag ccaaacatat aagatataac
     1321 taacgattaa attattactt atccaataaa aaatgactgc cggaaaaaag gtcaagtagg
     1381 gataaaaata attcactccg ctcccataat gattaaacgt cgtaaaattg attggaccag
     1441 aaaaaattga agataaccct ttgactctag caaaatggaa ggcaatatcc cccgatgtag
     1501 aaatagtatg aacatttcta aaataattga ttgaataaag tccagcaata attgctagga
     1561 gaatggacaa tattattttt ttctttactt gcattactta attatatact cctttcatgg
     1621 attgctttat ctagtataac ttaaatttta aataaatcgt tatttatttt tactctcact
     1681 taaaaaagca ctaaaattat aagagaggga ggtaatggtc tgaacaccaa gaattgttcc
     1741 aacatcgaat tgcttaacta atgagttcaa agcttaatta tgtggctctt atatttaccg
     1801 agcgtggatt gcgatctctt tagcgaaaca aacatctttc ataaaaaaaa cagccatatc
     1861 cgctttcttg gatatgacta tttactacta ctttactatc tttcattctt cttgtctcac
     1921 ttatgataag gcgcccccgc attgatccgg aaggcacggt agatttgctc gacaaggatc
     1981 agacgcatca attgatgtgg atacgtcatt tttccaaaag agatctgagc attgcttctt
     2041 ttcatgacgg cttcgctgag tcctaaagag ccgccgataa caaaaacaag gtggcttttt
     2101 ccttggatac ctaattggtc gatttgttta gcaaaagatt cacttgtagg atttttccct
     2161 tcaattgcta aggcatagac gtattcttgt tctttgattt tcgctaagat ccgttcccct
     2221 tctttttctt ttacttgtct catttgcgct tcgcttaaat tctccggggc tttttcgtca
     2281 ggaacttcga tcaattcgat ttttgcatag gcattcagcc gtttgacata ctcattgata
     2341 ccttggatca ggtatttttc ttttaatttt cctactgaga taattttgat attcatgttt
     2401 tctctccttt ggtcttcatg ctcactttgt ttattataat caattttttc gtctatatgt
     2461 ttattttggc tatttttgtt aaaaagattt ccccaacagt tatgcacaac atccacaggt
     2521 ttatccacag aaaacagtgt tattttacct tatttcatca cattcattag gcaaacatac
     2581 gttttataag aatttatcca ctttatacac aggctgtgga tatgttttat cccttatttt
     2641 tccaatccta tccgcgtagt tttacacagt atctgtggaa aaagaaaatc aaatattttc
     2701 atctgaaaac aatgattttt cctaaagttt ttcataaaaa gttcacagtc tcaaagtaga
     2761 ctaaagtcaa caaataagac tatactaaca gtagataaat gaatgtaatt ggaggaggaa
     2821 cacatggata gaaaaaatgt gacaccaaag atgaaaaaaa gtaaaaataa tagcctctgg
     2881 cgtaagttag gtctggggct tgttggaggg atcgttggag gactactgac agctggcatt
     2941 ttttatgcag cgatgggcac tggcaacaat agttctactg ctacaagcgg caatcagaat
     3001 tctgccggtg aaacagttgt ggaaaatgtc aaagtcaatg tagattctga tatcaccaaa
     3061 gcggtagata aagttcaaga cgctgtcgtt tctgttatca acttacaaag tcaaaaccaa
     3121 tcaagcggtt ttggcggact attcgggcag caagaagaaa gtagcagctc agatgatagt
     3181 aatttggaag cttatagtga aggaagcggc gtgatctaca aaaaagacgg gaatactgct
     3241 tatgtagtca cgaataatca cgtagtagat ggccagcaag gtcttgaagt cttgatgaaa
     3301 gacggtacga aggtaaaagc tgaattagtt ggaacagatg cttattcaga tttagcggtg
     3361 ttgaagatca attcagacaa agtcgaaaca gttgcctctt ttggtgactc aagtgctctg
     3421 aaggttggtg agcctgcgat tgcgattggt tccccactag gatctgaata tgcaaattct
     3481 gtcacttcag gaatcatttc ttccttgaac cgacaagtga caagtacgaa tgaatcgaac
     3541 gaaacagtca atatcaatgc gatccaaaca gatgcagcaa tcaaccctgg aaattctggt
     3601 ggtcctttag ttaatatcga aggacaagtt atcggtatca actcaagtaa gatcgcaagt
     3661 acttctgaat ctacttctaa tgtaagcgta gaaggaatgg gatttgctat tccaagtaac
     3721 gatgtagtga atatcatcaa tcaactggaa aaagacggaa aagtgacacg tccagctcta
     3781 ggtatcacaa tggtcgactt atctgccgtt tctactcagc aacaagaaca aatcttgaag
     3841 attccttcat ctgtaactaa cggtgtcatc gtgacaaatg tccaaacagc tacaccggct
     3901 gaaaaagctg gattgaaaca atacgacgtc attactaaga tcgatgacaa agaagtttct
     3961 tctggtgtag aacttcaatc tgctttgtat aagaaaaaag tgggagattc tgtcaaaatc
     4021 acttactatg aaggctcgaa agaaaaaaca actactgttg aattgacgat cgatcaatcg
     4081 gcattaaaac aaaatcaaaa tagtgaaaat taataaaaaa gaggttgtga caaagacttg
     4141 tcacaacctc tttattttgg aaatgcttct ccgactacgg catcctcagg caatactaag
     4201 attccttttt ttgctggagc atttggcaaa tgcaattcac gagcggaaca gatcattcca
     4261 tagctatcta cccctcgcaa agttcctggc cagatcatca ttccatctgg catcatagct
     4321 cctggttttg caacgacaac tttttgtcca gcttcgatat tgggtgcacc gcaaacgatt
     4381 tgcaatactg taccattgtc tacttctgtc tcagtaatcg ataaatggtc agaatcagga
     4441 tgtgggacgc atgtttttac gtacccgaca acgatcttcg gttcatgatc agcttcaagc
     4501 tgttcttcga atcctgcttc tttgatcaat cgattcaatt tcgctaattc ctcatctgtt
     4561 aagcttactt ggccgttccc ttcgattgtt agatgatcgg agatatggaa aaagttccaa
     4621 gcaacaactt gaccttgttc atctttgatc cgagcaacat tccatttgcg ttcaacagtg
     4681 ttttctgttc ctttatcgtc ggctataata accatcaagg tgtctccgac gtgtgctttg
     4741 ttataagcaa aaatcatgtt ctttcctcca ttatcctttg ctgagattta tcttaacaaa
     4801 aaattgcatt cactgcatga aatttttttc atgctttatt tccgaagaag cctcttctgg
     4861 aacaccattt attcgtaaca agggactgtg acaagacttt tgtcacagtc cctttactgt
     4921 tttagttaat tgattaaaaa ggtcgtacaa gttccacttg cttaattact tggctgttct
     4981 tcttcgtcag aaacgatcaa gacatcacaa ggtgaatgac gtatcacgta gctggctaca
     5041 cttcctgtca taaagcgttc gactgcattt agacctgact gcccaaccat gattagatcg
     5101 atctcatatt tttttggcaa tttgactgcc atggattcac gaacagaacc atatgcaata
     5161 attccttcta tatttgtgta atcaacagat ttcccatatt ctttacattc ttcgatcatt
     5221 tccttggctg tttcagtttg ctgatcgact agattttgat caataggtgc atagcccata
     5281 aagttataaa attgctgatc gattacgttt gctacatata ctttcccatt attacggcga
     5341 gccacttcta ccgctttttt gaaagcagca agtgcttgtt cactaccgtc gatacctact
     5401 aaaatattct tatattcttg attcatcttg acccctccta acagatgtca gccttttgtt
     5461 acactttcta ggaacgattc gatttcctgt tttgttttac gatctttatt cactagacga
     5521 cctaattctt ttccgtcttg gatcacgaca aaacttggga taccaaaaat gttccattca
     5581 gcggctacat ctatgtactt gtcacggtct acttcaataa attgccaatc agaaaagtca
     5641 ttttcaattt caggcatttg cggtttgata aaacggcagt ctccgcacca gccggcagta
     5701 aagaaaaata cactctttcc agtttctacg tagcctgcta attcttctaa actatttggg
     5761 ataatcattc tttgtcctct actttctgtt tatgtatttt tcaattaaat tatacacctt
     5821 tctttcgaac taacaaacat tgatcctctt tttataaaaa aaatctggca aaaagcaatc
     5881 ttttgccaga ttttatagtt tcactatcgc attattttcc gtatacgatc gtatttactg
     5941 ttgatttatc caaacctttc agtgtttgaa tcaacatttc tcgtgcagct tcaaaatcgc
     6001 gaatactgaa catcgtttga tgcgtatgga tataacgtcc cactacgcca atcactgtac
     6061 ttgggattcc ttcgttttgt gtatgagcag cacctgcatc tgtaccgcct tttgaaacaa
     6121 agtattgata agggatattg tttgtttcag cgatatccag caagtactcg cgcaaacgag
     6181 gaagcatgat caaacctgga tcttggatac gcatcaatgt accttctcct aaatgtccga
     6241 atgtgccatt tttcgtcact gtatcatctg ctgcagagca gtctactgca aagaacagat
     6301 ctggcttgaa tttattgaca gaagctttag aaccacgcaa gcctacttct tcctggacat
     6361 tcgcgcctgc aatcaatgta tgacctaatg tttcattttg caacgcttct aaagcttcta
     6421 ataccattgt acagccatag cggttgtccc atgatttact gataatattc tttccgttgg
     6481 ctgttttgat cgtttcagcg taaggaacga ttgtgtctcc tggaaggaca ccgtaactca
     6541 ttgcctcttc tcttgattca aacccagcat cgaacaaaat atcggttact tctactgatt
     6601 tttgtccgct tgttcctcgc aataaatgtg ggggaacgga agaagaaata catggataat
     6661 tcttaccagt agaggtttta agtgtaaaac gctgagcaga aacaacgtaa ggattccagc
     6721 cgcctagagg tacgacttta aaaagcccgt tttcttggat ttgtgtcagc atgaatccga
     6781 cttcatccat atgtgctgcg accattacac ggggtgcatc cgcttcttca tgatggcgca
     6841 gaccaaaaat gccgcccaaa ccatctaact gtaactcatc taccagcggt tccatatgac
     6901 tttccatata agcgcgaaca tcttgttcaa aaccgcttgt tccttgtaat tctgttaact
     6961 ctttgattcg ttgaaatgtt ttttcttcca tatgctattc gtctaaattt cagactaaac
     7021 tcctctcaga cttatattga tctgcggata aaatatgatt ttttctgcag accgatcgta
     7081 tttattatag cttatttttt ctggtagcgg ttatttattt caaagaaaat atgagagtgt
     7141 ggtaaaataa gcacaaggag taacaagtga gaagaaggag gtatgtccac atgtatgaag
     7201 aaagcgaact aagctatttt aaaggcgggt tagcagttgg aatcggtcta ggattattga
     7261 gcggggtagc ttcaacgatt tggtatcatc gcaaaaaagt attgtcagca gaccaagtac
     7321 ttgaaaatat caagtcatct tttctaaaag aaggtgcaat cgaaggttct tggatctctt
     7381 ttgaaaagga acctattagg aaattcgccg ttcggtcgag tgcttatcgg ggaggtcttt
     7441 ctcgaataga agatggcgaa actgtttatt atgaattttt agcagatgct catacaggaa
     7501 ccgtattaga aatttcaaga aaaaaaggtg caacagatta atacgtcaaa aaagtgagag
     7561 aacgaatacg cacccactat tcaaaagaga ggtatcatgt ccttcttttg tagtgggtgg
     7621 ttttttagat agagctttcc aaacatatat tccagtattt tctttttaaa taaatgatat
     7681 ttagcgggaa aaactagcat ttcctttgat tatgaggtat ttttaattta gcttttgttt
     7741 tctatagtta aggagagtga aaaatggaac aacaagaaaa aattgctgtt ctgattcctt
     7801 gttacaatga ggaagcaaca atttcaacag tcattgcaga ctttaaacga gaattaccag
     7861 aagcagatat ttatgtctat gataataatt cgaccgatcg aacatacgaa ttggccgttc
     7921 aaggtggagc aatcgtaaaa aaagaacctc gtcaaggaaa aggaaatgtg atccgccaaa
     7981 tgttctttga tatcgatgcc gactattatt taatggtaga tggcgatgat acttatcctg
     8041 cagaagctgt acatggtttg ctcgagaaat tacgttctgg tgaagcagat atggtcattg
     8101 gtgatcgcct atcaaatgga acctactttg atgagaataa gagacctttc cacgattttg
     8161 gaaataacct tgttagaaat acaattacta gaatgtataa aacaaaaatt cttgatgtca
     8221 tgaccggcta tcgaggattc aatcgtatct ttgtcaaaag ttttcctatt atgagttctg
     8281 gttttcaaat cgaaacggaa ttgacgatcc atgctttaga taaaaaattc aagttcgttg
     8341 aaatcccaat cgattatcgt gatcgtcctg aaggcagcga atcaaaattg aatacattct
     8401 ctgacggatt caaagtgatc atgatgatta ttaaaatgtt taaagattat aagcctttgc
     8461 ttttctttgg gatttggacc attttcttct ttttatttgg attggtcacg gggattcctg
     8521 tgattcgcga atatatgctg acaagcttta ttacacgcat cccttctgcg attttaagta
     8581 ctggattgat gactttagca cttctttcat tagtaacagg gttgattttg gatacagtcg
     8641 tcacgaacgc taagaaagaa tatgaattga atctatatca tgtttatcat gaataccaca
     8701 gtagaaagta aaaaaaggat caagactgca aaaataattt gcaattcttg atcctctttt
     8761 taattaatta tttcgccaaa cgatagatgg catctgcata gattgctgtt gctctgaaca
     8821 agtcatcaag actcataaat tcgtttgctt gatgcattgt atcagtatat cctgggaaca
     8881 tcgctccata agcaacgcca cgtttcaaca aacgtccgta agttccgcca ccgatgattt
     8941 gttcgtagcc tttttctcca gtgtggtctt catagacttg caataatgta gccaccaatg
     9001 gatcatccat tggtacgtaa tgtggttcca ttacgcgagc acctcgagta actgttgcgc
     9061 cttcttgccc cactgtttgt tcgatgtcac tttgcaatcc gtctactgtt acaccttttg
     9121 gaaaacggaa attcatattg atgaagttcg cttcctctgt accattttct tcaaaagcaa
     9181 atagtccagc attcattgtc aatttgccca ttttttcatc ttcataagcg actcctagtt
     9241 tttctccata aaaatcttca tggacataag cagctgctac atggataaat tgtttagcag
     9301 atcctaggaa ttcatagtta tctaagaaca atgccaagaa agaacctgca ttgataccag
     9361 attgtgggct tgcaccatgt gcgcctttac caactaattc gatttttaca tacgtattgt
     9421 ccgcttcaat cgttccgctt actggatttg cttcgatgaa ttggtaaaat gcttcttcca
     9481 tcgcgatagc tgcatctgca cttggtactt gaagtgctgc agttgctgtt cctggcacca
     9541 tattttcgcg aagtccggaa acaaaagatt tcaagacata atctcctgca ttgtcgcctt
     9601 tgaagcgtaa agctaaagaa acatttcctt tttcaccatt gatgattggg aattcagcat
     9661 ctggtgagaa accaaaatct ggttcttctt catgttcgaa ataataagcc atatcgcccc
     9721 agccgctttc ttcatcgctg ccgactacga agcgaacttt ttttgatagt ttcaagtcaa
     9781 gttctttaat gatcttcatc gcatagtaag ctgccatact tggacctttg tcatcgcttg
     9841 atccgcgtgc aaagatcttt ccatccttga ttactggttc ataaggatct gtgtcccagc
     9901 catctcctgc aggaacgaca tccatgtgtc caaatatccc tagcgtttcg tctccttcgc
     9961 caagatcaat atgccctgca tagttatcga cattttttac tttaaatcca tcacgttcgc
    10021 cataagctaa catatgtttc aatgcgtcgc gaggtccagg tccaaatggt gcatctggtg
    10081 ttacttttga gtcatcgcgc tcgctgttga cacgtaaaag atttttcaag tcttcaagca
    10141 agtcttcttt acgtgcttct acttcttttt gccaatcaat tgtcataaag ttaatcctcc
    10201 acttatttaa gtcttcgtgt tcatgatacc acaaattaca gaaaaaaata gttcaaaaaa
    10261 tctgacaatt ccttttctta tctgtttttc tctaaaaaaa atatacgcta taatgagtaa
    10321 aaggaggttt ttagatgctg ataaacgaac aactagtgaa aagagtcatc acacctcgtc
    10381 caaaaatttc acataaagga acatttggac gtatcgtttt gattggcggc aatgaacaat
    10441 atggcggagc aatcattatg agtgctttag ctgccgtaca cagcggagcg ggtctcacga
    10501 ctgtagtaac tgatgaaaaa aatcggagtg ctcttcacag tcatctgcca gaagcaatga
    10561 ttgtagtttg gacggatact gaccagtttc tttccgtaat aaagcaagca gatgtactct
    10621 tgattggtcc cggattgggt acttcttcta agagtttgga acgtttcaaa caagtactag
    10681 aaaaccaatc aaaagaacaa tggctggtga ttgatggctc tgctttaagt ttacttgctg
    10741 ctaatcatct tttgctaccc tatcccgaac aaactgtact tacacctcat caaatggagt
    10801 ggcaacggtt gagttctcta ccgatacaag aacaacggga taaagaaaac aaaaagaagc
    10861 aacaggaaat cggtgctgtg ctcgttttga aaagtcatga gacaaaaatt tatggtcacc
    10921 agcacttcta caccaatcct ttaggttctg ctgctatggc aacgggcggc atgggtgaca
    10981 ctttagctgg gatgatcaca ggatttttag cccagtttcc tcaaaatgac caaacgattg
    11041 cagcagctat ttatcttcat agtttcattg gagaccagct ggcaaaagaa caatatattg
    11101 ttttgccgac acaaatcagc gaagcacttc cgaaatggat gaattatttt tgtaaaaaat
    11161 caatggaaaa cgaggcatga gtcatggaga aatttacagg ttatgtgcga gaaccttttt
    11221 attatgaaac agatcaaatg gggattattc atcacagcaa ttatatccgc tggtttgaag
    11281 aggctcgggt agccttattg gtacatctag ggtttcccta taaaaaaatc gaagaatctg
    11341 ggatcatcat ccccgttctg gaagtaaatt gcagctataa agaaatgatc cattacggag
    11401 aaaaagtgag catccatcca actatccaaa agtatacggg gacacggttg gattttagct
    11461 acgaaattgt gggaactgaa gacaaaaaat taaaaactac tggcagtagt aaacactgtt
    11521 ttttactttc tgattcgcaa cgattagtga agctgagcaa agtcaatccg gagttagatc
    11581 agttattcca agcgtacgct tctttggatg atgtaaattc ttaataaaga ccgtctaagc
    11641 atttttcttg tattgtgtcg agtagttcgt ataatgaaaa gaaaaaagca ggtgatcccc
    11701 atgtatgtcg ttaaagtaat gcacggttac atcgataaaa ctggctgtcg cactcgggaa
    11761 aaaaacttag acaatctgct cattttcaaa gacaaaaaag aatccgaagc ttttgctaaa
    11821 agaatcggcg gtcgtgtcaa accgatccaa gaggtacgac cagattgatt caatattttt
    11881 cttttcgagg ctctaatagg catgcctatc agggcttttt ttgcagactg tgtttttatg
    11941 cccacacgat ttgtaagcga tttatgttat aatagattat atctgcatag aaaggaaggt
    12001 taaatggtta tcaataattt gcaacagcaa gcttatgaat ctatacgtaa acaaatcatt
    12061 tattccgagt tagcacctgg aagtaaaatc tccgataaag tgttagaaga acaactagct
    12121 atcgggcgta caccgattcg agaagcactg atccaactgc gcaatcaaga gcttgtaaaa
    12181 accattccac agtccggcac ttatgtttca aaaatcgata tccgatctgc ttcattagcc
    12241 cgttatacgc gggaaaagtt ggaaaaaccg atcctgcaag aatgcagtgc gaaaatgacc
    12301 aagcaagatc aagctaaact agaaaaaata ctagaacaaa ccgatcaggc agtaatcgct
    12361 caaaataaaa aagctttttt tgatttggat actgattttc accgaacttg ttatgaaatt
    12421 gctggaaaac gagaaatttg ggattggcta gaaagttata gtacgcactt gaaccgtttc
    12481 agatggttac gtttgacgat ttcagaatta gactggggtc gagtattaga tgaacatcag
    12541 acaatgcttc aatcaatgat tgatcataac tttgatgaag tcggatttct ttgcacgatg
    12601 catttacata tgattatcga ggagcaagaa tacgtgattc ataattaccc cgattatttt
    12661 gaagatattc aggacagacc catcaaataa gaggttttgg aatcagcgtt ttgacctgag
    12721 tattagagaa agaaacggca aaatagttcc tcatattttg ccgttttttt tgagctaata
    12781 ccgagggtca gctgattgca caccgtttat tgagaggttg tgaaaaagct gccctgcatc
    12841 aagtaataag aacagccaag taaaaatagc ttcttatgtt tttaacttgg ctggacttat
    12901 taccgcagat gcaagctttt gaacaccgtt ttccaagagg tcgtgagcct gccgttcatc
    12961 tccgagtcac aaggaacaat ttcaaaaaac agcttctcat gtttgttgaa tattttgact
    13021 tgtgaccgag gagttgacag gagaacatcg tttattcgga aaaaaaggac tgtgacacga
    13081 cttttgtcac agtctcttta tttgtaatct aaatcatact ccgtacatct tttttctatt
    13141 ttttttattt cttctaagat cccttggcta cgaatttcat tatatggcca aagtaagact
    13201 gcctctaata cataaggcga aaaatcgctt tgattcatct cttttgaaaa actcatcgct
    13261 gctatctcgc tcaatgcacg aatgtttgat tgatgtgtat aaaaaggcaa ccgccacagc
    13321 acaatttttt cgctttgcgt ataaattccc ttaacagaag cttcgatgtg gtgaaatagt
    13381 tcatgtccaa taatcaaagc ttgcgctgtt tctttagaga tcgaagctag tcctgaacat
    13441 tttaattttt ctaacggctc cgtcatcaat cgaatccctt catcttctgc ataagttgca
    13501 aaagtgattc gttcgtttat ttgctctgat tcacaggaaa cgattggaac agccatttgt
    13561 tcacaaattt ctcctacatc ggcaaaaggg tacttttttt tgatgctaca tgccgtttcg
    13621 ataccacaat cgattgattg ttgtataaga attttctgct tggacaaagg aatctttttc
    13681 caaaataaat ctcgccgaaa ctggtagaat ccccaaattt cttcggggat ctgttgtaat
    13741 tcgtttagta actcttctaa cttatgtttg tttgatattt ccggcaacga cgatcacttc
    13801 ttcctccggt tccatagtag atacttccag atccatcaaa agctgtagtt gttccagatc
    13861 cgtcgctgta aaatcagaga ctcttgctcc taagcagaga taaaattctg gtcgggcaat
    13921 caattcagtt tgataaactg ccatatcttc ccaaagttgt tcgacagctg tcatataatt
    13981 ggctgcagtt gttttcatac atgaagcatg tccacgttgg accttgataa atcctttttg
    14041 atcgatgatc cgaagacttc cagcattttt tgaacgattc tgttcgccgc atacataaaa
    14101 gaaaggtgta ttttccaata agcaaacttc atttttattt aatcgcatat cctctgcagc
    14161 aagctctagt gcttcttctg ttgtgatctc cttggttaga tcggttgctt tcacttccgt
    14221 agaaccagta gcgatagccg ttacttttga agtctgtgga tcgatttcta cgtggacttc
    14281 gatcgattct ggtgtagcgc ctgattcaat cgctttattc atcgcttcat ttttcaacga
    14341 tcgtatatct tctttacttg gcgaagggat gatacgttct acaacatctc ggaccattgc
    14401 aagtgctaca ccaatagaag aaatcacttc ggcgttttca ggaatcgaat atttcacacc
    14461 cattttatta ctaaagtaag taatcaatga ggcagcgccg ccccctacac ctacaagaga
    14521 aatctgatct ttctccaagt gatacttgtc tgccaattct aaaatcaccg gttcaatctt
    14581 agcataggat ttttccatga tctgttctgc gatgtcttca accgtcgtat gacaataatc
    14641 agctaatgct tggatggctt ttcgtgcaga tgggacattt ccataagaaa aatgttcttc
    14701 ttgcactaat ccaaggacat tggctgcaca tgtatttgta atagtcactt cttctccgtc
    14761 ttccatcact accttgacgt aatcagcagg gtctccttct ttaggagaga aaaattcaac
    14821 cttaggacct ttgatttttt ctgtctccgt aaatactgca taatctagac cggcaatatg
    14881 tgcagaacgt ggaccgacat cgatgattcc tgactgatta gcccgtacca tcgaaccgcc
    14941 tgccacacct agaacccgaa catctaatga agagatatat gttgggtggc cacctacaat
    15001 tgaataatcg attgctggac gtccattttt aatgaccccg atattggttg ttgttccgcc
    15061 tacttcaaaa taaacaccat tagacgctct caagtacatc aaagagccca tgactgatgc
    15121 tgctggaccg gaaagcatgg ttaaaacagg tcgttttttc atttcattga tttccattac
    15181 tccgccgtct ccgcgcataa tcatcaaaga aacattgacc cctgcttctc gtacggaatc
    15241 ttctgtagag gttgccgtat ctagcatttt tggtaaaata gatgcattga ttgctgctgt
    15301 ccgagttctt cttgtcaatc catataattt tgtgatgtca gaagccatcg ttgtagggat
    15361 cgattttacc gatgctgctt cataaacaac tcgttctggt tccccattat ctactccaaa
    15421 tgccatagaa gaaaccaaga cctccgctct ttctctttct aaagaagaaa tcgtttctga
    15481 aacacggtct acatttaagt gtttgacagg taagaatgca ttgacaatct ctattttttt
    15541 cttattccct aaatcgatat cgttcaatct agtttgtcgt tttgctaaaa aaccttctaa
    15601 tccacctttt gccatcccga ttacgccgac ttttgctacg tctccttcaa tcagtgcatt
    15661 agtcgcttgt gtggtagaat gtgcaacaaa cacaacatct tctggactga tattattttc
    15721 cagcaaacaa ttttgaaaag actgaacgac acctgcggct actccacgga catcatcgtg
    15781 ggttgttttc actgaagatt ttccgatgat ttcgtgcgtc gcattatcga ttgcgactgc
    15841 tttcgtatgt gtaccgccaa catcaattcc cattcgcaca aaacgtttca ttttcttttc
    15901 ctccattcat ctaatcgttt ttatcgacgt tttgtttact aggcgttccc gtacaataag
    15961 aacgtgatca ggcataagat cgctccgatg atccaaccag ttgggatgga tttcttcata
    16021 tattctcgag aagttacttt tgtataacct aatccccaag caacccaaga ttgtgtaata
    16081 tctaaatgct gaggagcaat cgttgtgatt gcaaataacg ggaacaaaaa ggctggcgac
    16141 atcgttggat taacagctaa tacgacagct aaaatagctg agcctgaacc gactagattc
    16201 attggaccgc ggaaaaatcc tagtggtgtc aatatagcaa atacgattgc caagactaat
    16261 ggtgattgcg gtatccaatc tccaaaaata gctgaaaaat aaggagaagc atacgcagct
    16321 gaattattga acatcgctaa ggtcatcaag aaaccaacca ttggcgcaac atctactgcg
    16381 ccatctgtaa ataatttcgc aaactgacga cagatttctt tataacctcc gcgcaatttt
    16441 ccagtaaaca gcaatgccca gatacctgct agaataaaac ctaagatgat tggaagatct
    16501 aaaagaacga cccctaaaac tggtaataat acggctaacc atgagatcat tggtgcgtcg
    16561 ccaccttcag ctggtacgtt tgctgccatt gcatagcgtt tctttttgtt cattgatata
    16621 ttagcgaccg ttagtaccac aactaagctt acaatcatcc caatcatacc aatttggaaa
    16681 tagtcattat aagtatagct ttcagccgct ggattgaaac cagcataaat cgtttgatat
    16741 tgtttgaaat tgacgatatt agcaaagatt ccagccataa tcgagcccat aaatgagaac
    16801 agtgcaaccg gtactgaaat acctaatgac atcaagattg gcaacacaat aactgcaata
    16861 gaaatcactg gtccgattcc tgtcatcgac atgaatataa cagcggttac aatacatagt
    16921 aagctcatcg tgattcttgg cttgtcccct ccaagttcaa ctacctttcg aatcagggta
    16981 gctgcgattc cgctatctac tagaacacgc ccgaaaaagg caccaaagaa gacatttacc
    17041 aagatcgatt tggcgtactc agctggcccc gtcgtataga tatgcgtcaa cacatcaatt
    17101 actccttgcc cttccatcgc cgggtttggt gaaaacgtgt ttccgactag cgctaatccc
    17161 atccatacag tagccatgat ggcaaagccg accatcaagt tgtagccttt tacacagtac
    17221 cagaccatga aaaagaatga gataactaac aaaataccaa taataatatc actcatgtta
    17281 ttcctccgtc cattttgttt ttctgataag caaactaata tatcagtcaa actcattcta
    17341 acttatgtag ttagcgcttt cattaaaggg tgaccataaa tgttcagttt tcaattaaaa
    17401 aaccttaatt atgctatttt agacttatac gttattgtcc tgtttcattt gatggaggat
    17461 tgtaaataaa aaaagaggaa agaacataac ttttcgttat cttctttcct ctttataatt
    17521 gtttagctta tttagccaaa gcttcttttg cttggtcagc caatgcagta aatgctgctg
    17581 catcgttaac agccaagtca gctaacattt tacggttgat gtcgatttca gcaactttca
    17641 aaccgtgcat taattttgag tagctcaagc cattcatacg agctgctgcg ttgatacgag
    17701 caatccacaa tttacggaag tcgcgtttct tttgacgacg atcgcgatat gcgtagttgt
    17761 atgaattcat tacttgttct tttgctgttt taaataaagt gtgttttgaa ccgtagtaac
    17821 cttttgctaa ttttagcact cttttacgac gtttacgagt tactgttcca cctttaacac
    17881 gtgccatgta taattcctcc taataaaaat aaatcttcag attctgttat ttgtacttct
    17941 tatttcattt ttgctagttg ttggcgaata cgtttgaagt cgccactaga aaccatgctt
    18001 gctttacgca attgacggcg ttgttttttt gttttgccgt ggaaacggtg acttgtaaac
    18061 gcgcggaagc gtttcaatcc gcctttacca gtacgtttga aacgttttgc tgatccgcgg
    18121 tgtgtttttt gttttggcat gactatattt cctcctcaaa atctttcgtt atccgactat
    18181 cggctgtcca acagccagtt agtcattcaa attacttact gtcgtttttc ggtgccagcg
    18241 tcaagaacat gctgcgtccg tccattttcg ctttttgttc cactgttgca atatccgcag
    18301 tttcttcagc taagcgattc aagacttttt gaccaatttc tttatgggta atggcacggc
    18361 ctttgaaacg gatagaagct ttcactttat cgcccttttc caagaactta cgtgcattac
    18421 gaagttttgt attgaagtca ttcaagtcga tcgtaggact taagcgaact tcttttacat
    18481 tgatcacttt ttgtttttta cgcgcttcgc ggtctttctt ttgttgttcg aaacggtatt
    18541 taccgtagtc catgattcgc gcaactggtg gtttcgcacc aggggcaact aacacaagat
    18601 ccaagtttgc ttgttctgca atattcaatg cttctgcttt tgtcttaact cctaattgct
    18661 caccatctga accgatcaat cgtaactctc gtgcacgaat gccgtcgtta accatcatat
    18721 cctttgctat ggtcattcac ctccaaatat tatgagagaa agaaaagctt accgaataaa
    18781 cggcaataaa aaaacgagtt ctcttacaga acccgcgcgt acttcaccta atagaaccag
    18841 tgaaaactat ctagcccagt gacattatca actaaggcga gaagcgggtg cttctgcttt
    18901 tatttctcaa ccatcatagt atagcaaaaa ctaatctttt tgtcaatgtt tttcatacag
    18961 tttgtcttcc ttttctgaaa atgattagat tagcagactt tctagcagtg attcccgctt
    19021 ctttgctata ctaaagaaaa acgaggtgaa cccacatgaa aaaaatcgaa cctattccac
    19081 cttctcttcc tcttcttaca aagggagagt tttatttaga agatgaggag atcttctctg
    19141 gtcttctgat caacgggggt gaccaaagct atctcgatgc caaaaatatg gtattaagag
    19201 agagtcatat ccaaaaactt acgatgcaaa aaacagtttt acgtcatttt gaatgcagta
    19261 atgtcatttt tgaaaaatgt gatttttcca atctggagtg gattggtggc agtttccacc
    19321 aagtcgtgtt tcatcaatgt aaattaacag gtacgaattt tgccgaaagt tatcttcgag
    19381 actgtacttt cactgattgt attgcttcga tggcttcctt tagtagtaca aacttgaaag
    19441 ctgtctcctt cgatcattgt caattagaag acagcgaatt ttatgaggtc acttggaaaa
    19501 accttttttt gtctgacaat cagttgaccg gttcgaactg gtttcgaact tctctgaagg
    19561 gattagactt tacgactaat ttcttcacag caatcgcttt atctcaagat tctttggcgg
    19621 gactaatcgt caaccaagag caagcacttg tgatcgccca agcccttggt ttgcagatca
    19681 aagactagat caacgaatcc gccgccacag tgcaataagc atccaaatag ataagaccaa
    19741 actgatgatc aaggcgattc cccatccgaa aacggaatgg gtaaagggta actggagatt
    19801 cattccgaag aatcctgtca caatagttgg aacggtcaag atcagcgacc agaccgtcaa
    19861 aaatttcatc gtgtcattca agttgttatt cagcaaatta ttataagtgc cagagagctg
    19921 gtctaaaatc tgcgaagcta agttcgtcat ttcaactgct tgtcgtgctt cgatcagcgc
    19981 atcgtccaac tcttcttttt ccttttctga caacttgcga taaatagcca atgccttgat
    20041 ttgttctaaa agtactacat tttgttttgt tcctgtaact aagtaaacca aaccgatctc
    20101 taaatctgaa agttgcagca gattcttgtt cgttgttttt tctcttaatt tcagattaag
    20161 ccgctgacgt tcactattca cttcctcgat taaaggaaaa aaggaatcag aaatcaaaaa
    20221 caatgtacga aacaataaac tgtatacact catgtctggc attcgttcca aaagcgatcg
    20281 aattagtcga atcacgtact cggtattcgg cgtggtaaat gtaaacaaac cgtcttcttt
    20341 taaaataaaa gcgataggac tagtttcata atgattatcg actttttgct ggtgcggaac
    20401 atttgataca agcagtaacg cttcatcaaa agaatcgtac tctacgcgcg cccgttcatt
    20461 tttatctaga gaataagtca gcatctcgtc actaatgcca tattcttctt ttaagttttt
    20521 aacagctagc acatcgtttt gctgtacatt gatccaaaaa ttacggtctt gattaaaata
    20581 gtgtttttcc cgcatactct atcctcctga catttgtttc cagtataaag aaaaaagtcg
    20641 ctcaaataaa ggtttaccct ttatttgagc aactgtaaat caaatggcta ctttggtttg
    20701 gcgaatctct gctaattttt cttcgatcat tttcaagact tcttcttttg cttgaggatc
    20761 ttccacaaaa ttatagcggt ctccatcgat ttggattttt ggactttctt tgtattcttc
    20821 ataccattga tcgtaacgtc tgttcaattc tttgtaataa tcgtatagac ttggatcaaa
    20881 ggacaattgt tcatacgaac gaccacgttt ttcgatacgc tccaacatcg tatcaaaaga
    20941 tacacgaata tgaactaata aatccggatg ttttttatgg gcagcatagg gaagttcttc
    21001 catcatgttt tccagcagtt catcatacac acgaacttct gtttctgttg ctcttcctag
    21061 atctgcattt agatggaaaa gcagtgaatc ttcataaatc gaacggtcca atacattatt
    21121 gtcatctttc attgcttgtt tgatgcttgc aaaacgttta ttcaaaaaat aaatttgcag
    21181 caaaaaagca tactgttcag ggtttgcata aaataagggc aagacttcat tatcatcaat
    21241 cgattcataa aatgcctgac tatcaaagtg ttcagccatc atttctgtta aacttgattt
    21301 tccggcacca attgtacctg ctaaaacgat cacgctcatt tacctccagc tcacttcatc
    21361 actaaatatt gtgtttaact ccaacctttt taaatatacc cccaatatct agtagtgtca
    21421 attgtatttt tttctagtca aagaagaaaa aaagtcaaaa aattcttaag aaaaaagtgt
    21481 tttttcttta gaaaacgtat tcttagtttt acttctgaga tggttttagt aaaataaagg
    21541 aggaatgttt tttgagaccc ttagtggaca aaaaaattcg tgaagcagcg caactcgttt
    21601 tgggcgcttt attttttgct taaaaatcac ttttacgtga gaaggagtta gattatggca
    21661 aactatggcc aaggagaatc aagcggaaag atcatattga tgggcgagca cgcggttgtt
    21721 tatggagaac cagcgattgc ctttcctttc tatgcaacaa aagtcaccgc attccttgaa
    21781 gagctggatg caatggacga tcaactggtt tcttcctact attcaggaaa tttagccgaa
    21841 gctcctcatg cattaaaaaa tatcaaaaaa ttattcattc acttaaaaaa acagcatgac
    21901 atccaaaaaa acttgcaact gaccattgaa agcacgattc ctgctgaacg tggaatggga
    21961 tcaagcgctg cagtcgccac agcagtcact cgtgcttttt atgattactt agcatttcct
    22021 ttgtctcgtg aaatactatt agaaaatgtc cagctttcgg aaaaaatcgc ccatggtaat
    22081 cctagtggaa tcgatgcagc cgctactagc agcttgcagc cgatttattt tacaaaaggg
    22141 catcctttcg actacttttc tttgaacatc gatgcttttt tgattgtcgc tgatacagga
    22201 atcaaaggac aaacaagaga agccgtcaaa gatgtcgctc acctctttga aacccagcct
    22261 catgaaactg gacaaatgat ccaaaaatta ggatacttga cgaagcaagc aaaacaagca
    22321 atcattgaaa attcaccaga aacgttggca cagacaatgg atgaatcaca atcacttctg
    22381 gaaaagctga caatcagcaa tgattttctt aatctattga tccaaacagc aaaagatacc
    22441 ggggcattgg gtgccaaatt aactggcggt ggacgcggtg gctgtatgat tgcattagca
    22501 caaacaaaaa caaaagccca agaaatcagt caagcacttg aagatgcggg tgctgctgaa
    22561 acttggatac aaggattagg agtacatacc tatgtttaaa ggcaaagcac gcgcatatac
    22621 caacattgct ctaatcaaat attggggtaa gaaaaatgaa gaactcatcc ttccaatgaa
    22681 caacagcctt tcgttaacat tggatgcatt ttatacagag acagaagtca tcttttctga
    22741 tagctatatg gtagatgaat tttatctaga tggcaccttg caagacgaaa aagcaacaaa
    22801 aaaagtcagt cagtttcttg acctttttcg taaagaagcc ggcctctcac taaaagcttc
    22861 agtaatcagt cagaatttcg ttcctaccgc agctggatta gcctcctctg ccagcgggct
    22921 agctgcttta gcaggcgctt gcaatactgc tcttaagctt ggattagatg atctctctct
    22981 ttcaagattt gctcgacgcg ggtctggttc agcttgccga agtattttcg gtggtttcgt
    23041 cgaatgggaa aaaggccatg acgacttaag ttcttacgct aagccagtcc cttccgattc
    23101 tttcgaagac gatttagcaa tggttttcgt tttgatcaac gaccagaaaa aagaagtgtc
    23161 cagcagaaat gggatgcgtc ggacagtcga aacatccagt ttttatcaag gctggttaga
    23221 ttccgttgaa ggggatctat atcaattgaa acaagcaatc aaaacaaaag atttccaact
    23281 tctcggagaa acgatggaaa gaaacggact aaaaatgcat ggaacgactt tagctgccca
    23341 gcctccattc acttactggt ctcctgacag cttaaaagcg atggatgcgg tgcgacaatt
    23401 gagaaagcaa ggcattccat gctactttac gatggatgct ggccctaatg tcaaagtttt
    23461 agtggaaaac agccatttat ctgaggtaca agaaacattt accaaattat ttagcaaaga
    23521 acaagtaatt actgcacatg ctggtccagg aattgcgatt attgaataaa gggagatttt
    23581 tatgattgaa gtatctgcac caggcaaact ctatattgcc ggagaatatg cagttgttga
    23641 aacaggccat ccagcagtta tcgctgcagt cgatcaattc gtgacagtca ctgtagaatc
    23701 tgcacgaaaa gtcggaagta tccaatctgc acaatatagt gggatgcctg tacgttggac
    23761 aagacgcaac ggagaattag ttttagatat tcgggaaaat ccttttcatt atatccttgc
    23821 tgctattcgc ttgactgaaa agtatgcaca agaaaaaaac attcttttat ccttttatga
    23881 tctgaaagta acaagtgagt tagacagttc aaatggccgg aaatatggat tgggttctag
    23941 cggtgccgtg accgttgcaa cggtcaaagc cttgaatgtt ttttacgcgt tgaatttatc
    24001 tcagttggag attttcaaga ttgcagcact agccaattta gcagttcaag ataatggttc
    24061 ctgcggcgac atcgctgcta gctgttatgg tggctggatc gctttctcaa ccttcgacca
    24121 tccttggctc caagaacaag aaactcagca ttctatcagt gagttacttg ccctggattg
    24181 gccaggtcta tccattgagc cattgattgc tcctgaagat ttacgtttat tgattggttg
    24241 gacgggtagc cctgcctcta cttctgattt ggtcgatcaa gttcaccgtt cgagagaaga
    24301 taaaatggtg gcttatcagc ttttcttaaa aaatagtaca gaatgtgtca acgaaatgat
    24361 caaagggttt aaagaaaata atgtaacgtt gatccaacag atgattcgaa aaaaccgaca
    24421 attactgcat gatttatctg caatcactgg ggtcgtcatc gaaacgcctg ctttgaacaa
    24481 attgtgtaat ttagctgaac agtatgaagg agccgcaaaa tcttctggtg caggtggggg
    24541 cgattgcgga atcgtaattg ttgaccagaa atctggcatt cttcctttaa tgagtgcatg
    24601 ggaaaaagca gaaatcactc cactgccgtt acatgtctat agcgatcaaa gaaaggaaaa
    24661 ccgatgaatc gaaaagacga acatgtttca ctagctaaag cctttcatga taaacaaaaa
    24721 aacgaatttg acttcgtacg gatcatccat aaccctttgc cgcaaatagc agtttctgat
    24781 gtcgatctaa gtacacaggc agttgggttt acactaagca gcccatttta tatcaatgcg
    24841 atgactggcg gaagcgaaaa aacaaaaaaa atcaaccaag atcttgcaat cgtcgcaaga
    24901 gaagcagatt tgatgattgc cactggttca gtcagcgctg ccttgaaagt cccttcttta
    24961 gccgatactt atacgattat gcgacaagaa tatcctcacg ggaaaatcat tgccaacatc
    25021 ggtgcaggga cttctgtcga aagagccaaa gaagctatcc gattgtttca cgcagatgcc
    25081 ttacagattc atttgaatgc acctcaagag ttggttatgc ctgagggaga ccgtgatttt
    25141 accaattgga aagtacttat tcaagaaact caaacagcca ttgacgtccc cctcatcgta
    25201 aaagaagtcg gttttggcat gacaagagaa acgctcaatg atctagctgc tttaggagtt
    25261 catacagttg atatcagcgg tcgaagcgga acaagcttca cccagatcga aaacgcccgt
    25321 cgctccaagc gggaactgag ttatttagct gactgggggc aatcgacagt ctcttcattg
    25381 cttgaagcaa atgaagcaga cacttctatg gaaatcctag cctccggagg tatccgcaat
    25441 gcctatgaca tatttaaagc cctttgtctc ggcgcaaatg ccgtgggtac ttcaggaacc
    25501 gtactgacgc atctgatgaa tcacggcgta gaagaaacta ttatactgat gaagcaatgg
    25561 caagaagagc ttcgtctgct ttatactatg gtcggcgcaa cgaataccgc ggccctacac
    25621 caacaatctc tgatcttttc cggaccagtg aaggattggt gcgaagcaag agggatcgac
    25681 ttagtgaagt atggaagacg cacagaaaag agcgtgggac aaaagtaaag aacacttttg
    25741 tctcacgctc taaacacgta caaacggcgg aagtaaaagc aactccttcg gaaataagct
    25801 gaaattcaca aaaatttgaa aagcaatttt cgtgaattcc ttcttatttc ttggagttaa
    25861 atgcttctgt cccagcctct tttttcgaat aaatgatgtt taatcagctg tccttcggta
    25921 ttcgttcaat ccgctgattt cacaacctct ccactaaaca gtgtactaaa gctcttttaa
    25981 ggttgttttc cgatataggc aagtatgcct ccgtccacat acaagacatg gccgttaacg
    26041 aaatcagatg cgctagaagc caagaagaca gatggtccag ccaaatcgat tggctcgccc
    26101 cagcgtgctg ctggcgtacg tccaacgata aattgatcga atggatgacg ttcaccgttt
    26161 tcttgtgttt cgcgtaatgg agcagtttgc ggtgtagcaa tataacctgg tccaattcca
    26221 ttacattgga tattgtattg accgtattca gaagcgatgt ttttcgtcag catttttaat
    26281 ccgccttttg ccgcagcata agcactgacc gtttcgcgtc ctaattcact catcatggag
    26341 cagatattga tgattttacc gccgcctttt tcgatcatat ccggaataac tgcttttgaa
    26401 acgatgaatg gggcatttaa atccacgtcg attacttggc ggaagtctgc cgcatccatt
    26461 tcgatcatcg gtgtccgttt gatgatccct gcattgttga ctagaatatc aactgaacct
    26521 acatcttctt tgatctgttg aaccatcgct tgaaccgctt cttcatcggt cacatcgcag
    26581 acataacctt ttgcatcgat gcctgcttct ttataatgcg ctataccttc atcaactgat
    26641 tcttgattca agttattaaa tacgattttt gctcctgctg tagctaaaga gcgtgcaatc
    26701 tcaaatccaa taccatagac agcaccggtg actaatgcta cttttccatc taaacgaaac
    26761 atatccatac taaattccat cattttctct cccttatgct tattttaatt gatccatttg
    26821 caccatatcc atgtctgtat aggtgatatt ttctccgcac attgcccaga tgaatgagta
    26881 attgcttgta cctacacctg aatggatcga ccagcttggc gaaataatgg cttgttcatt
    26941 gtctacaact aaatgttttg tttcatctgg tttgcccatc atgtggaaca cacgtgtatc
    27001 ttcattggca taatcaaagt agacataagc ttccatacga cgttcatgtg tatgacaagg
    27061 catcgtattc caagcgcttc ctggttctaa aatcgtataa cccatttgta attgacagct
    27121 ttcgcataca tttggatgga tgtattggta gattttacgt tcattcagtg tcaggccttc
    27181 accagtctcc attggtttta cttggtcgat actgattttt acatttggat atttatgatg
    27241 agctggaaca gaactaatat agaatttagc tgggttttca gcatcttgag aagaaaatac
    27301 aacatgtcgt gtttcttttc cgatatagta tccgtcttgt tttttcattt cttctttttg
    27361 cccatcgatc tcgatgaatc ctggtccacc gatattgatc acacctagtt cacgacgctc
    27421 taagaagtaa tcaacaccta gttctttgtc taatgtgatt tctaattctt tttcagtagg
    27481 tgtcactcca ccaaagatca ttcgatcgtt gtgcgtataa gtcaaactga ttttccctgg
    27541 ttcaaatacc ttttctacta aaaattcttt tctcaattgt tccgttgaat aatgacggat
    27601 atcttctgga ctgtgtgtat aacgtgtttc catttcttgc attttcttct actccttcta
    27661 tcgaatgatt tttccagagc cgcatgctaa aaattgtgcg acttcttctt gactaaattg
    27721 gttgcagtcc ccatggatcg tatgcttcaa ggcagaagct gccgtagcaa aatcaatgat
    27781 ttcttgcgga gacatattgg ataaaatccc atggagcacg ccacctgcaa aagcatctcc
    27841 gccacctact cgatccacga ttggggtgat tctatggact tgggattcca catattcatg
    27901 gttcatccac aatgtcccct tcaattcatt ttcacttgct gaatgaacgg tacgcttggt
    27961 cgaataaaac acctgaatat tcgggaactt ttcctgcatt ttttgataat aataaattag
    28021 aggttgttct tctgcttctg gcgctttctc gatacctaac aaataaagag catccatttt
    28081 tccagctgag cagatatcta ctaaaggtaa aagctgatgg attacttcgc tggcttcttg
    28141 ctgtgtccat aattttccac gatagttgat atcgaagctg atcaggcagc cagctttttt
    28201 tgcagcttct atcacctgtt tcgtcatttt ttgccatttt ttagacaaag ctggggtgat
    28261 tcctgaaaca tgaaatatat ctattccttg gaataactcc tctagcgacc attccaaatg
    28321 atccatttca gcaaaacttg atccggcacg atcatagatc actgaggcgg cgcgttcgcc
    28381 actaccagtt tccatataat aagtacctag tcttgatcca ccagataata cctgtttcgt
    28441 atctactcca taacattgca agtgcttttt gactgctttt cccaatgcgt tatcaggtac
    28501 ttttgtagca aaaatcgctt catgtccgta attagctaaa gaaattgcta cgtttgcttc
    28561 ccctccgcca taatgggcag cgaatcgttc acttgaatcg atacgggttc cctgctctgt
    28621 ggacaatcgc aacatgattt ctccgagtgt cactactttt cccataagca gctacctctt
    28681 gatttctttc atttttgctg catactgttg cgcgacttcc gttactttgt caaaatctcc
    28741 tgtagctgcg ggtgctaaaa ggttcccacc tactccgact gttacgacac cagcatcgaa
    28801 ccactctttc atattgtcca gactcacacc gccagttggc atgatattga cttgaggcat
    28861 cggcgccttg aaggcagaaa tgatacttgg tccataagcg cttccaggaa atagtttgac
    28921 gatatctacc ccgcttttca gcgctgtttt gatctcggta atagtcatac atcctggtaa
    28981 gtaaggaatt tgatacagat tgcacatctc tgccgtttct tgatcaaagg aagggctaac
    29041 aatatactct gcgccagcta agatagccaa gcgagctgtg acagcgtcta atactgtgcc
    29101 tgcgccaatc actatttctg gttggtcttt gtagaatgac aaaagttctt tgattacttg
    29161 atctgcttga gggactgtaa aagtcaactc aataccagtt agaccacctt tgatgattgc
    29221 gtggcaagca ttcaaagctt cttctttgct ttttccgcgt accaccgcaa tcactccggc
    29281 gtttttcaat cgagataaaa tatcaatttt tttcatcttc tccacctatt tcctatttag
    29341 aaattttatt ttataacttg gaaatctata cccatattat acggcagaaa agaaaaattg
    29401 caaacgcttt tttcattatt tttgaattct ttgctcgatt tcgctttttg tttgcttgag
    29461 ggcactgatt atttgttctt ttgtctcatc tgttaaacga tatttcggca tacttacact
    29521 aaatgcacca atgatttgtt cgtttttcac gattgaggct cctatacaaa aaatatcacg
    29581 ttcgatttct tcatcgtcaa atgcgatgtg ggtttctttt accgttttga tttccttttt
    29641 caaccgtaat gcattggtga tcgtatattc tgtgtaaggg atcaacggat gcttttcaag
    29701 ataatcagtg tactgctctt cactgaattc tgctaatacg gctttgccca tcgcagaatt
    29761 gtataatggt cgggtgattc cgatttttga agacatgcgg atcgtttgat gttttggatc
    29821 tagtttgttc acgtacaaga tctcattgtt gtccaatacc cctaagtgga tagtctcatc
    29881 gatcttttgc tgcagctgtt ctaaaaacgg taaagtcaac tctaccaaat cgatttgttc
    29941 aatgttttta ttcgcgtagc gaataagctt tgcacctaaa cgatagtttt tctctgaatt
    30001 tttatttacg tagccgatca tcaacaaagt atctagtatt ttcaagactg tagaagaggt
    30061 cattcccaca ccctttgcga tcgtctgcaa agagacatct tggttttccg ctaaatagtc
    30121 tagtatttta gaggctttga tcaatacagt tccataaggc tttttatttt ccatataata
    30181 ttctcctatt tctttataag aaaaattata gcggaaatac catcaattta aaagagatat
    30241 ccagatataa actaccattt ctctttacag gcaatttaat gttgaagatc cgcttcgttt
    30301 ttacatcaaa ctttgatata aagcaatgat ttcttctact gtcgtttctc ttggatttcc
    30361 tggtgtgcat acatctcgca aagcatcttc tgcaattgct ggaatatctg cttcttttac
    30421 acctaattca gaaatggttt ctgggattcc aaccgctttg ctcaaccgat cgatctcacc
    30481 acaagccgct tctcttacat cttctaaaga catttcagct gctcctttaa tccctagaac
    30541 aagagcgatc tcacggtact tttctccagt atattctttg ttatatttca tgacagttgg
    30601 caaaagtgct gcacacgcta cgccatgagg aatgttgtac catgcactta acgggtgagc
    30661 catcccgtgt accagtccta aaccgacatt tgagaagccc attcccgcaa tatactgtcc
    30721 taatgccatt ttttctcgcc cattttgatc gccatctaca gaagcttcca aagaatggcc
    30781 gatgatttcg attgctttta aatgcagcat atcagtcatt tcccaagcgc cttttgtgat
    30841 aaacccttca atagcatgcg tcatagcatc catacctgtt gatgcagcca attttttcgg
    30901 cattcccatc atcatatcgc tgtcgatgaa cgcgacgatt gggatatcat gaggatcaac
    30961 acaaacaaat tttcggtgat ttacttcatc tgtgatgacg tagttgatcg ttacttctgc
    31021 tgctgttcca gaagtcgttg gtatagctaa tataggtaaa catggatttt tcgtatcagc
    31081 aacaccttct aaactaatga cgtctgaaaa ctctggattc gtcaaaataa tcccaatggc
    31141 ttttgccgta tcgattggcg aacctccacc gattgcgatc aaacagtctg cctctgcctc
    31201 tttaacaaaa gccactccat cttttacatt ttggatcgtt ggattaggta cgatcccgtc
    31261 gtataaagca taagcgattg ctgctttatc tagaagttct gttacttttg ttgctatacc
    31321 gtgctcgatc aatcctttgt cagtaatgac agctgctttg ccgaatcctc gctgctgaaa
    31381 ttcagggacg atcgactcaa tcgctccttt tccaaaatac gatgtttcat ttaaaatcat
    31441 tcgattactc attttctatt cctcctaatt tgtattattt ttcacaataa aaacgaaaga
    31501 accccaagca accggttgcc cagttgcttg ttgctacagt tcaaagactg gcatcaattc
    31561 ccaactgact ggagaggcat ctggatttgt ctccatgata tcttccatgt acttccacca
    31621 tttttgattg atagatgtat ttgccatttt ttcccataac ttttcatctt cgatcactaa
    31681 gtaaccaaat aacaaacttg tactcggatc aagatagatc gaatagtcga tgaccccgtg
    31741 ttcacgaagt gctgtacgca tttctggcca aagttcatca tgtctttttt tgtattcttc
    31801 ttgtttttct gggtaaactt tcatgcaaaa agcttttttc accatgtcag acacctgctt
    31861 tcgtatgaac ttcctccaaa taacctgctt ttggagtgac accaaacgca tctgctaaat
    31921 gccacaggtt ttcgtccgtg attgtttgca agattggtcc ttgcgctttg acgtacgtat
    31981 agacttctgc tgccttttct gctgtctcga tcaaaccaaa gacttcatcc atgtcttttc
    32041 ctgacccgta gattccatgt tgtggccata aaaccaatcg agtttctttc attttttcag
    32101 ctgttgcttc cccaatctca tttgttcccg gaaccatcca cggaatgatg gaaattcctt
    32161 ctggaaaaac aactaagcat tccgtacaca tttgccatag cgtacgacta aaggaacgtt
    32221 cgtctaattc atgcgtaaaa ctcatcgcta atagatgact agcatggcaa tgcataacga
    32281 tccgattatc tgggtctact tctaaacggg cgatatgact catgaaatga cttggcaact
    32341 cgcttgtagg aaggcattcg ttttccagcc cccacaaaag ttccagtgtt ttgccatctt
    32401 cagcaactcg aacgattcca agattctcgg caggttcgct cgctacattt ttaaaatatc
    32461 ttcctgaacc agttacaata aaatagcatc ctgctaaacg gctggcatca aaaatcatcg
    32521 agatgcggcg gatcactttt tcaggatcca agaacggtgt gatctcttct tcttttagta
    32581 aataagaaat attcccgcca tttctttcat cccatcccaa gcgataaaga ttcgacgtcg
    32641 tttcgatcat ttctttcaca taaggcgctt gtaaaatatc tatttttttc aatatagcca
    32701 cactcctatt ttcttaattc caacacgtct ttttgatatt cctggacttc ttccagccaa
    32761 tccaaaccaa ctggaacttg atttttgtaa cagaaataat tccatacatc ggcatacggg
    32821 aaatctttca attcttccgt ataagccatt cttgaagtaa agtcaaaagc cagctccatt
    32881 tctttcaatc gctcaactgg ttccagcata gctttcagca gtgctttttg tgtatttctc
    32941 gtaccgatga cccaagcagc gatacggttg atcgttgcat caaagaaatc cagaccgata
    33001 tttgtttttt ccaacaattc gttacggact aattctttag cgatttcctg caattcatca
    33061 tccatcaaaa ccacatggtc tgaatcccaa cgcactggac gggaaacatg caacatgatt
    33121 cctttgctga ataaagctaa tgaagataat ttattggaaa tcacttctgt tggatgaaaa
    33181 tgtccagcat ctagacaaac aagtttgtca cgagtcaacc cgtaacccat ataaaattca
    33241 tgggaaccga ctgtataagc ttcagcgcct aaaccaaata gtttgctttc tactgcatct
    33301 tgtacatacc gttcatctaa ctcttctgta aaaatttcat cgagtgattc cattaaacgt
    33361 tcccgtgggg taaaacgatc aatcggatta tctttcatcc catcaggtac ccaaaagtta
    33421 ttgatacttg tttgcccagt ttctttccca aagtactccg cgattttgcg gcttcttttc
    33481 ccgtgttcga tccagtattc acgaacttct ttatctggat gagccaatgt aaagccgtct
    33541 ttcatcattg gatgcgaaaa gaaagtcgga ttgaagtcaa gaccgatttc ttcttttttt
    33601 gcccactcta cccatttttc aaaatgtttc ggctcaattt catttagatc gacagattct
    33661 tctgtatcca aatagatagc atgaaggttc aatttgtgtt tgcccggtat caacgaataa
    33721 gccttctcca gatcttgtct aagctcttca ggtgttcgtg cggctcctgg aaaattccct
    33781 gtcgccatga ttccaccggt caattcgcca tccggtgtca agaaaccttt gacatcatct
    33841 ccttgccagc aatgcataga aattttgatg tcctgtatct tttctagtac agcatctgta
    33901 tctacaccga tagttgcata tttttctttt gcttgttcgt aatttctttc aatcgtgttc
    33961 atacgattac tcctttgatt ttattcacgt accattccat cgtgttttgc ttattttcat
    34021 ctgggtaaaa agtttcacat ggaaacgact gtttgattac atttcttgct tgttctatat
    34081 tcgaaaactg acctgtactg agcatttgca tggcaacatt cccgattgct gtggcttcac
    34141 taggtcctgc ttctacaatg attccggcat aatctgctgt caattgattc agtagacgat
    34201 tattcgatcc gccccctacg atgtgcagct tttccaattt cgttccagtt aagtgttcta
    34261 ctttgatcag ctcttgtgcg taacaaagag caagattatc atacacacaa cgagcgatag
    34321 ctcccgatga gacagggact ggctgctctg tttcaaaaca gtattgctga atctcttcta
    34381 tcatatttgg gggattcaaa aatcgagggt cattgatttc catcacttgc tgcagcggtt
    34441 tttcttttgc agcaagctct gccaattccg cataactgta ctcgtactct tgcattcggg
    34501 caacttcctg gatcaaccac atccccatga tgtttttcaa aaaacgcacc gtgttatggg
    34561 caccccattc attcgtataa ttttcagaaa aagcattgcg agaaacattg gcgatctttg
    34621 tttcaatccc taaaagcgac catgtcccac tgctgatata accccaacgt tctccttgac
    34681 ctggcgttcc taatacagct gacgccgtat catgacttgc tactgtgata aaggtcacat
    34741 ctggcaaatc atattttgaa aattgttctt tttgcagatc tcctagtatc gttcccggct
    34801 ctgtcaatgg tggaaataat tccttcgaga caccgatttt ttcaagaagt tctatttccc
    34861 attcttttgt ccctgcattc agcagctgca tagtagatgc attagttttc tccgtcacca
    34921 tgttaccagt aaagacatac cctaaataat caggtatcag taacaattta tgtgcttttg
    34981 ccaaattttc tttttcttca gcaaacaatt ggaacaaggt attgaaaggc tggatctgaa
    35041 tccctgtctg ctcatacaac ttatctagag aatagatttc agaaaactgt tctactgctc
    35101 cattggtacg cttgtcccga taagcaatcg gatcatttat ccgatcccct tgttcatcaa
    35161 tcagacagta atcgactgcc catgtatcga tccccgcaaa aaggcgatca atccctgtct
    35221 gctttaattt ttcaaatcca atcaaaatct ctttgataag ataatcgatc ttccagcgat
    35281 cgtgaccgtc tttgttccga aagccgtttt tgaaccggtg tacttcttcc aagatcaaag
    35341 aaccttttgc atccctcgta cttttcatta gtcgtccgct tgatgcaccg atatcgatgg
    35401 caacaaatgt tggattcatt tctccacctc ttttacttta ttgttgatta gcactttatt
    35461 aataaagcgt tatcatttat tgacaatgct atcataatac agaatcttat attttgcttc
    35521 acactatttt ggtatataat tgcattatct tgacaagttg ggagtgttta catggattac
    35581 tgggaaaaag ccaaccactc atggtcggaa gactcaaaaa gagtgatctt gacgcctaca
    35641 caaagaagca ggaatttatt tttttatatc caagaaatcg gttacttcaa agcaccaatc
    35701 ccttactata ctgaacgagc acatttacct tcttacttga taaagtttac tttgtctggc
    35761 aaaggttcgc ttacatataa tgataatcag caacagctaa aagctggaga cttatttttt
    35821 attgactgcc aagaatatca gcgttatcaa acgatcagcg aagaaccatg ggaaatggat
    35881 tggatccatt tgaatggtca gaacgtccaa tcattttatc aggagttcac aaaagacggc
    35941 cataacacct tccattcaac aggagatcca tcagaaaaca agatccacca tttgatcaca
    36001 gaattgatcc acatgcaaca aggcacgaat gcccgtaccg atttccagtc ctctgtagct
    36061 atccacgagt tgctgaatga actgattttg caaaagtatc agctagattt cgaagaagag
    36121 gatattcccg cctacgttta caaactgaag caatacttag acgaacattt caaagaaacg
    36181 attacacttg atcaattaga acaacaattc ttattgaata aatatcagct gagcaaagat
    36241 ttttccaaat ttatcggttc tccaccaatc gattatttga tcagcaaaaa actttcttac
    36301 gctaaagacc ttcttcggta tacagaattt acggttcgaa tgatttccga agaaataggg
    36361 attgagaatt ttgcttactt tagccgcctg ttcaaagcaa aaacaggact atcgccgaga
    36421 gactttcgta aattcagctg attcatataa tatgtacgtc attttttcaa aatattttga
    36481 aatatataga taaaaataaa ataaccgcag tttctgcttc tatgtaaaca acagcagcta
    36541 ctgcggttat tttttagtga tcattctcat tgttcatctt caaaatgttc ataatgaacc
    36601 aactgtttct gtgtatcacg tttcaaaaat tctttaggcg ttatgccgaa ctctctcttg
    36661 aatgctttca gaaaatgcgg ataagaagaa aaaccacttt tactatatac agcactagag
    36721 ctgcggtatt ctcgcaacaa gtgcttggca tagatcagct ttttcttcat tacatactga
    36781 tgaaacgtaa atccagtgta ctttttaaat tctcgtgtga tatggtattt gctgacaaaa
    36841 aactgctttt ccatttcctc taaagaaagc ggatgagata aattttttga gatatattgt
    36901 aacatttgtt ccacacgctc attaggaact acagcattct gttcttgcgc actctcttct
    36961 ttttgtacaa cttgattgag aaaaatcaag tattctacca gagcttgttc atattttatg
    37021 tcttcaccgt aaattttttt atcaggagga tggtcgataa atgataattt ttgcttcaat
    37081 acttcctgtt ctatttgcaa tacacgactc ctcttctctc ccattggttg aaaacaagcc
    37141 gatagattag accatggcgt ggaacgactt tctagaaaag aaggggtgac aaagatatag
    37201 acacgctcga atacatctga agattggcgg acgatacgat gcaaatcatt ggaatggatg
    37261 agcagcacac ttcctgcttt gatcgtaaat tgtcgtccgt tcaaataaaa agtcgcttct
    37321 ccctttaatg tagcatggat ctcatagaaa tcatgatgat gatagatcgt tttattttgg
    37381 ctgacgacat ctttgacatg atagatttca tactgatcag aaaccatcgt attgacttgt
    37441 tcaccgctga ttttcttttg gagcatgtgc cgcacctcct tttgttcact tatgcacaat
    37501 tttattatac cacgtttttt gtaatcaaaa agagctgctt ttatatactc ttataaaaaa
    37561 gaagagacgt ggcatcttct agccccgccc cctccattct aaatattagt taaccaacgt
    37621 cttctttaca ttattcaaga agacttcttc cataacgaat cccatcatct tttgtttctt
    37681 caagaaccac atacaggtac ggacgattct tcatgatgat ttctttgatt tttgtgtact
    37741 cgatcacacc gtctcccaaa tttaccggca cgattttttc ctgttcgaca cgaaaatctt
    37801 tcaaatgaac cgcacatatc ttttcaccaa aacgttcaaa agcttcttct accaattgga
    37861 cttgttcttg ataatcacta cttgtgatca aattggtcgg atctaaaatg attcctagat
    37921 agtccgaatc tactgcttgg atcagctgct ctacccttgc taaagaatac accggatgat
    37981 tcaaacctgg ttctatcccc accatcattt ggtatttttc tccagctttt accaagcgac
    38041 ggataacgga cacagcttca gcaaatgctt catctgtgaa attttcttcc gtatattgga
    38101 tttccggcaa aacacatcca gtttccgaag caaccatcga cgcaccaaag gaagaagcat
    38161 agcgaagata ctgctcgaac tttgtcagca actcttgtcg tatcgttaaa tctggatgaa
    38221 tcatattgat atagcaactt aaaattccta cggtcacttc ttgcttttct aaaactcgtt
    38281 tgacataatt tcccatccca gaattgatct catttgcgcc agaaggtatt tcaggaaacg
    38341 aaagacctaa ggcgagctgc agtgtatgaa tcccttgttc ttttgttttt tctgcaagat
    38401 cttctatcgt ttggacttcg gaaagatcat gtcctctaat tgtaatgttc aacataacta
    38461 ttctcctaac tcagcatatt gggtagccac atgacgattt gcgggaagta agtaatcaaa
    38521 aacaagacga ctaagataac cccataaaaa ggaagaagcg gtttcagcat tgcctctgct
    38581 tttacgcctc ctacacttgc accgacatat agtcctgtac ctactggcgg cgtgatggag
    38641 cctacacaca gattcatgat tgaaaacaac ccgtaatgta caggatcaac ccctacactt
    38701 gtgacgattg gtaaaaatat tggtgtaaaa atcaagatag caggcgccac atccataaac
    38761 atacccacca gtaataacac aagattgact aataacagaa taataaattt attatccgtt
    38821 acgcccataa tcaacgaact aattgcctga ggaataccgg taaacgccat ggcgaaagac
    38881 atcatggaag aagtagccaa taagaacatg atcgtccctg acattgctac tgattgaaac
    38941 aacatattag gtatatcttt tagttttacc gttttatagt aaaacatagc aatcaacagg
    39001 gagtaaatga cagctatcgc tgaagcttct actgctgtaa atatccccgt caagatacca
    39061 ccgataatga tgacaatcaa tagaaggcta ggaatcgcct cttttattat ttttccagca
    39121 tgagccatct gtcctttttc gacaacaggg tatttattct ttttagcaac gatgaatgct
    39181 actagcatga ttcctagcgc ccacaaagcg cctacgagat aacctcccat aaacaaagca
    39241 gagatcgaag caccaccagc aacaagtgaa tacatgataa aagcagtact tggaggaatg
    39301 accatacctg tcggagctga cgcgatattg accgctgtcg caaacttccg gtcatatcct
    39361 tcttccactt gctgagggat caacactccg ccaatcgcgg tagaagcggc aatggccgag
    39421 ctagagatgg aaccaaataa agcattccct aacacattcg tatgagccaa agcccccggc
    39481 acgcgcccga cgaacaacat cgcaaaatcc actagtttct tagctatccc tccattattc
    39541 atgataatgc ccgagagaac gaaaaacgga atagcgatca gcgaaaaact attgatactt
    39601 cccaccattt tttgagctga actaaaagca gaaacatcaa acggtataac taacaataaa
    39661 gtcatcatcg atgagaatgc aatacttata gcaatcggca tccctaatac aagcaacaca
    39721 acaaatacaa aaaataacac taatccggct tctattgcca ttttattcct ccatttttat
    39781 attttttctt gaattgctgg cttgttgctt aatgtatcga ttagattgat caggctatag
    39841 ataatgatga gtacccccga tacaggtact gacagataaa catatcccat aggaatactt
    39901 aaagaaggcg agatctgccc cattgtcaat gtgacagcat tccatccgcc atacaccatg
    39961 atcatcgcag caaataccag aaataaactt tgaacaacaa cttctaaaat tttacttttt
    40021 ttgccagtta atttttcact taaaaccgtt acggctaaat gtgctttttg tcccacaaca
    40081 tacgcggaag acaacatggc aaaccagaca agaccgaatc gaacaagctc ttccgtgacc
    40141 gtatttggat ttcctaaaac cgttctggaa aatacttgcc ataaaacaac agcgaccatc
    40201 ccaacaagca aaacagctcc caggatctct aataccttgt ttaaaatcat tttcatcaca
    40261 gctacgctcc ttgctcttgg atcttttgat aaaggtcttt gtaggtagga tcgtttttga
    40321 atatatcatg aagtggggca actgcctctc gaaacggtgt attatctaca tcattgaatt
    40381 ggactccatg ttcttcttgt gctaaagctt tttcttcttc aattgctttt gcaaaagctt
    40441 caatttcaaa aaccgttgat tctttcgctg cttcttcaat ggctttttcc tgctcatcag
    40501 ttagctgttc tttgatggca tcattcatga tgatgatatc tggcacacgt gtatgctcat
    40561 cataggaata gtatttagcc acaccaccat gtccagctgt atacaagaca aattcattgt
    40621 tttctgatcc gttgatcaaa ttggattgaa gagacgtgta cacttcatca cttcccatag
    40681 gtacgggcga accgcctaaa agctctacca ttttgatagc ggtttcgctt tgcatcacac
    40741 ggatcttctt tcccttcaaa tcttccggcg tatggatcgg accgtctacc atataaaagc
    40801 tgcgctgacc agaatcataa taggtcaatc cggtaaatcc taaatttttg gtcgattggt
    40861 agattggctc cattatttct tcattatcca tcaccttaaa gaaatgttct tcatcattga
    40921 atagataagg aactgaaaaa atcgaataat ctttagagaa actttccaac gctgaggcac
    40981 ttacttttgt gaaatctact gctcctgact gtgtcaattc gatcagttca cgttctccac
    41041 ccagttgtcc atctgggaaa tactggatct gtacggaacc atctgttttt tcttttacta
    41101 aacgcccaaa ttcattcatt gcatcaatta ctggctggct gttgcttgca taagcaaagc
    41161 gcaaggtaac tatctcattt acatctgcat ctgcgacagt tgccacacta gaattatttc
    41221 cgcagccgct tagacctagc agacctaggc taagcgaact tgctgcaaac agcagtgatg
    41281 tttttttcat atgggtttcc tttcttttaa caataagaga cgagcaattc tgtcaaacaa
    41341 agcaccgtta acgactgtcc ataaggcatt gctgtgattc cgatattccg ataaaaatct
    41401 aaactgtccc ccattcctgt cccgattgaa acgttttgaa cttctccttt ttcatcgatt
    41461 tgttcaagca atccttggat agcacgataa gcgacttctt tatattcttg tggtaaataa
    41521 cgtttatgga ccgctttcaa aataccgtaa gcaaatcctg cagttgcaga agattctacg
    41581 taagaagaag gatcatccaa caacgtatgc cataaaccgc tttcagactg ataagttttt
    41641 aatgcacgca cttgtgcttc aagtgtttcg atcaataatt tgcgcaatgc atcttctttc
    41701 gataattcta agatttcaat gatctctgga atagcaatcg tcaaccagca gtttccacga
    41761 gcccaaaatg cttcggcata gttatgattt ccttcaaacg tccagccgtg ataccacaat
    41821 cctgttcgtt tatccatcaa gtatttgata tggatcataa actggtaacg agcttcttct
    41881 aaatattctg ggcgattcaa cagtttcccg atttttgcca aaggaagcac tgtcatcatc
    41941 aatgtatcgt cccacaactg attcttattt tccggtccat atgtggcatg ctgtaacccg
    42001 tcttcatttg tccgaggcat gtcattcatg acccattctg cccattgttc taaataaggc
    42061 aaatagcgtg aatctttcgt gtcttcatac aagtaagcca tagtcaatag cggtgccatt
    42121 gtattgacgt ttttgggcgg agcgccttct tccatacgat cctcaaacca ttcattgacc
    42181 acttgataag cgcgaggatt ttttgtcatc tggtaatttt tataaatacc atatagaccg
    42241 acaccttgtg gccaattcca cactgtccag cttttgtcat ctactttcag tccatcaaag
    42301 tccaacaaaa attcaccatt atcgtctttg atcgttgtca ggttttcaac gagcaggtca
    42361 attttttctt tcactaattc tttaggtaca aaaatctctt ctttaataat catctttacg
    42421 ccaccttttt aaacgctttc attttatatc cttaatatag cagtaatacc gtccactata
    42481 aatatcgctt tttgaacaaa aactgtctaa aattgaccta aatgttaaat aacaaaagta
    42541 actttcagtt acttggaaat taaaaaaaga acttggagaa acaacgtttt ccaagttctt
    42601 tttcatctat tttcttatct tttttctacg ctttcttttg cttatccgtt gtaagcatca
    42661 atgatgatct gtttcaattc agagatcaat ggttctttcg gattagctgt cgtacattgg
    42721 tcttcgtaag ctaattcagc catacggtct acggttgtat ctagtgtttc ttgtgtgact
    42781 ccttgagctt ttagactcat gttgatgccg acagattttc ctaattcata aacagccgtt
    42841 gctaaagctt cgactaattc ctctgttgtt tcgcctttca gacctaagaa ttttgcgata
    42901 tctgcgtaat ctgtatctgc acggaaatag tcatatttag ggaacatcgc atgtttttgt
    42961 ggatcttttg cattatagcg gataatatgc ggtaaaagaa tcgcatttgt ccgtccatga
    43021 gggatcccgt attccccgcc gattttatgt gcgactgagt gacaaatacc taggaatgcg
    43081 ttagcaaacg ccataccagc cattgtagaa gcgttgtgca ttttttcacg tgattccata
    43141 tcaggtgttt ttactgattt ttctaaatag tcaaagacta acttgattgc ttgtaggctc
    43201 aatccacgtg tataatctga agccatgact gaaacatagg attcgattgc atgtgttaac
    43261 acatccatcc cggtatctgc tgtgacagat gctggaactg acatgacgaa ttgaggatcg
    43321 acgattgcga catctggcgt caatgcataa tctgctaacg gatatttcac atgtgtttca
    43381 ctgtcagtga tgaccgcaaa tggcgttact tcagaaccag tccctgatgt tgtcgggata
    43441 catacgaatt gtgttttttc tggtttgtcg atcttatatg tccgtttacg gatatccaag
    43501 aatttttgtt ttgcaccaaa gaaagatgtg tctgggtgtt catagaacat ccacattccc
    43561 ttagctgcgt ccattgcaga accgcctcca agagcgatta ctgtatctgg cttgaagtct
    43621 gccatcagtt tcgttcctgc gtatacggta tttgtcgatg ggttcggttc aacatctgag
    43681 aagacttcga tcttcacatc atttttacgt cgtgccaata cttcacgaac tgtatcgcag
    43741 taaccgaatt ggaccatacc agggtcacaa acgatcatga cacgttcaac gttttccatt
    43801 ttctctaagt acaatagaga gttcttttca aagaagattt ttggcggtaa tttgaaccat
    43861 tgcatgttat ttctccgttt cgctacagtt ttgacattga ttaagtttac agcagagacg
    43921 ttacgagaaa cagagttctt accataagaa ccgcatccca aagtcaatga cggaatcatt
    43981 tcgttataga tatcaccgat gccgccttct gcagatggtg tattcactaa aatacgacaa
    44041 gctttcattc gaagaccgaa tttgacatgc aactcttcgt tttctgaatg gataactgct
    44101 gtatgtccta atcctccaag attcaacata ccttcacaca aatcaaacgc atggtccgtg
    44161 ttgttagctt tgatcatggc aagaactgga gaaagttttt ctcttgatag cggataatct
    44221 gggcctacgc cttccaattc agcaatcagc attttcgttc cttttggtac actgattcct
    44281 gccaattttg cgatttccat cgcagaatgt ccaacgattg atggattgac cgcatatttt
    44341 ccttcgttca tcactgcatc ttctaatttt tgcaattcat ctggttttac gatataaact
    44401 tggtgtgctt ggaattctgc ttttactgct gcatagattt ctttatccac gatcacagct
    44461 tgttcagaag cacaaatcat tccattatca aatgttttcg aaacgatcaa atcgttcact
    44521 gcacgtttaa tttttgctgt tttttcaata taagctggta cgttacctgg acctacacct
    44581 aaagctggtt ttcctgtaga gtaagcagac ttgaccattc cagctccacc tgtcgctaag
    44641 acgatcgcga tacctggatg gttcattagc atagaagtcg cttcgatcga aggatgttcg
    44701 atccattgga tacaattttc aggagctccg gcagcgatag cagcatcacg taccactcgt
    44761 gctgcttctg cagaagattt ttgggcactt ggatggaaag caaaaacgat tggatttctt
    44821 gttttcaacg cgatcattga tttgaaaatc gttgtggaag tcggattcgt tgttggagtt
    44881 actccgcaaa ccacaccgac tggttcagca atttcaatca accctttttg gacatcttca
    44941 ttgatcacac caactgtttt atcatgtttg atattatgcc aaatatattc ggacgcatac
    45001 atatttttga ttgctttatc ttcgtaaaca ccgcggcctg tttcctctac agccatcttc
    45061 gctaatggca tatgttgatc caaagctgcc attgccattt catgcacgat atggtctact
    45121 ttttcttgat caaagctttc catttctttc aatgctgcat ttgctttttc tgccaaatca
    45181 tcaatcattg cttgtacgtc aattgtcttc gttttttctt tttctattac tttagccatc
    45241 attatcctcc taatgggtca tggttatttg ttaaataatt cacatgtata tcatacaata
    45301 tttatttttc tatgtaaaca cttttttgtg atttttttca cagttatttt taaatataaa
    45361 attgctttat ttacaggtgt ttgtatagaa aaatatataa agttatttat tattatttat
    45421 atgacacacc cgattattca atattgagca atatatccca tgctcttatc tctttctttt
    45481 ttgaataaaa aatgaggtag gacatctttc tgccctacct cgtctttaaa taagctttga
    45541 tacttatttt tcttccttct cttctgtctc ttccagcgtt tctgttgttt cagtttttac
    45601 ttcaactgtc tcagctggtt cttctgttgt ttcaacagta gtcgttgcat catttgttac
    45661 gacagtccct ggtttgaccg ttttgattgc tgcacgatcg tactcaagga agatcccttc
    45721 gcagtctaaa acaactgtcc gtttgtcatt gtcgatttct gaaacaactc catgtaatcc
    45781 gccgatcgtt acgactgaat cacctggttt catgctatct aacaagtttt gacgttcttg
    45841 ctgttgtttc ttttgcgaac gtgacataaa aaaccacatt gctaatagcg cgataaacat
    45901 cacaagcatt ggtaatgttc ccattttttt cacctcacta attctgtatc tacaaaaaac
    45961 actttagcag aaaaatgctg ataacactag ttttttactt aatttttaaa agattttcct
    46021 ttagaagctt tttgcgtttt ctttattgaa cccgtattct tcaaaaaagg cttctctaaa
    46081 ttcaagcaaa ttatcatcca tgattgcttg acgtacttgt ttcattagat tcaacaagaa
    46141 gtataaattg tggtaagaag tcaaacgaat cccaaatgtt tcatcacatt tgatcaaatg
    46201 gcggatatag gcacgtgtat agtttttaca tgtataacaa tcacatttct catcaattgg
    46261 acggaaatca tgggcatatt tcgcattttt cactactaga cggccttgag aagtcataca
    46321 tgtgccattt cgagcgattc gagtaggcaa gacacagtca aacatgtcta ctccacggat
    46381 aacaccatcg atcaatgaat cagcagcacc gacacccatc aagtaacgtg gtttgttttc
    46441 ggggatcaac ggtgtagtga attctaatac acgattcatt tctgatttag gttccccgac
    46501 agataacccg ccgattgagt aaccagggaa atccatcccg actagatctt tcgcgctttg
    46561 acgacgaaga tcttcaaacc ctgctccttg gatgattcca aataatcctt gacggtctgg
    46621 attagcatga gctttcaatc ctcgttctgc ccatcgagac gtacgttcta ctgatttttt
    46681 cacgtagtcg taactttcat cgaatggtgg acattcgtca aaactcatca tgatatcaga
    46741 gcccaattta ttttggatgt tgattgcttt ttctggtgat aaaaacatac gcgaaccgtt
    46801 caagtgattt ttgaaatgca cgccttcttc ttcgatattc cgcatatcac ttaatgaaaa
    46861 aacttgaaag ccgcctgaat ctgttaaaat cggttgatcc cagttcataa atttatgcag
    46921 tccgccagct tcttctacta agtcttcacc agggcgtagc cataaatgat atgtattact
    46981 taaaataacg ccagcaccca tttctttcaa ctcttctggg gacattgttt ttactgtagc
    47041 taatgttcct actggcatga acattggtgt ggggaaagtt ccgtgtggcg tgatcaattc
    47101 ccctaaacgc gcgccagtat gtttttcttt cttgattaaa cgatagcgaa tggcaggttg
    47161 cgtcattcat ttccctactt tctatataaa atatcttgac aactcccacc atcataatcg
    47221 tttcaagtgc tttttgcaag ttccctcggc ttccttctac tttatttaat attctttttt
    47281 ttatcctttt tttatgaata acaaaaaaaa gaaaaacatt atgacagaaa tattttttga
    47341 tacatggtat attggtgtta ttaaagaaaa ggagggaaaa atcatgcaat cttctgcaga
    47401 attaaaacgc caagcaaaag agagtctaaa aggacgttgg ggacaagctg ttttactaaa
    47461 tctaatccct acattgattg gtattgctac tattcttctt attatgattc ctattatctt
    47521 gtttgccacc accatatatg atggatcagc gagcgtctca gaagcgacaa cttcaggagg
    47581 cggtagttca ggaggcggtg gcggaattat ttccagcatc attagcgctt tgttcatgag
    47641 cggtatttct tggacttatc ttgatatttt acgtggacaa aaagatcaga tcgaaccatt
    47701 caaagatgca tttcgtggat tcaaaggttt gtttatcggc ggagtgatca gcttagctgt
    47761 gctgatctcg atctttacaa cattatgggg tttgctatta gttattcctg gaatcattaa
    47821 aagctattct tattctcaaa gctattttat ttactacgat atcgtccaag aaacaggaca
    47881 aacacctaaa gtactggata cgatcactgc aagtcgaaaa ttgatggatg gttataaagg
    47941 acaacttttc tggttagatc tttcatttat cggctggcat atcttagcat tagcaactgc
    48001 tggtattggt tatctatggc tcagcccata catttctgca acaaaagcag cattttatga
    48061 acaattgcca aaagatattt aaaagagagt gtgacttgtg accgaggggg tggcaggaga
    48121 acaccgttta tcgagaggca gtaaaagaaa cattcagccc attagcaaaa aggaggtgtg
    48181 acaacattca tgtcacacct cctttttgta tagttccagt aaaaagaaaa tcaacttaga
    48241 aacagttaat cattcctctt cttctgcttc cggtaatcga tggaacttga tcagttcatt
    48301 caccgcgatt ggtacgatag ataaaatccc taccaagaac cagtgattca aactgatgtg
    48361 tactaggccg aatagttctt gcgtaaatgg cagcaaagca acagccgttg taatagctaa
    48421 tgccagtaac gccatttcta ccaaagcttt attggtcgct aaattgactt tgaagatcga
    48481 ttggctgctg cgtacattga acacatgcag aatcgatgaa taagctaaga tcaagaatgc
    48541 aacagtttga ccgacatgct gacttgcgtc tacccatggg ctgatagtat cgacataact
    48601 acctaaataa taaccaaaca aggttacgat ggtgaagacc cctgcattta tggcaatttt
    48661 ttgcaataaa cctcgagcaa agatcccttc atttttcgga atgggtggct gatccatgat
    48721 gccgtgttcc gctggctctc ttcctaaagc aaatcctgga agtccatcag caactacatt
    48781 gacaaacagt agttgcacag ctgtaaatgg tgctccccat cctaacagca tcgcaatcaa
    48841 aacaataaag atctccgaaa tattacagct tagcaaaaag ttgatagctt tacgaatatt
    48901 ctgataaacc gaacgtcctt gactgacagc gtctacgatc gtcgcaaagt tgtcatccgt
    48961 caaaatcata tctgcggcac tttgcgccac atctgttcct gtgatcccca tggcacaccc
    49021 aacgttactt gctttcaacg ctggtgcatc attcacacca tccccagtca tcgctacgac
    49081 tgcacccgta cgttgccaag atttgacgat ccggatcttg tcttctggcg ttacccgagc
    49141 atagacagag agagctttga cacgcgcatc taattcttcg tctgataatt gatgcagttc
    49201 tgatcctgaa agtgcttctg acggatcatt caagatacct aattctttcg ctatggcact
    49261 ggcagtcacc acatgatctc cagtgatcat gactgttttg atgccagcct ttttcgctcg
    49321 cttgattgct cctttacttt ctggacgtgg tggatcgatc attccgatca gaccaatcaa
    49381 acgtaagtct ttttccagtg cttcagaaga aatatcttgc ggttcttcgt cataagtagc
    49441 ataaccaaca gcaatcactc tcagtgcccg tttaccaaag cgatcattaa cgatcgccgc
    49501 ttgttctaca tcgcctgaac taaagcgcgg agcaagaaca tcgaatgctc ctttggtgat
    49561 tgagatgtat ttttcgccca tttggtgaac tgttgtcatc atttttcgtt cagaatcaaa
    49621 aggaagctcg tttactcgtg gatatttttc ttctaattct gcctttgtat gataattctc
    49681 ttcaacagcc cttacgatgg ctacttctgt tggattacct ttgataacaa ggtcgtcatc
    49741 ttcttgctcg accgtcacat ctgtacatag tgcagccatt ttcaatattt ccatcgcgct
    49801 gttcgtcatt gaatcttcga tattcgttac ttcatcttct ttgctccata cccggcggac
    49861 gcgcatttca ttttgtgtca gcgttcctgt tttgtctgag cagataacat tagcggttcc
    49921 tagcgtttca actgctggca actgtcggat gatcgcatgt ttcctcgcca tcttctgcac
    49981 accgtatgca agtgtcaacg taacaataac agtcaaggtt tccggtacag ctgcaacagc
    50041 caaggaaatc gaagtcatga acatctcaag gagtggttct ccttgcagtt caccaatcac
    50101 aaatactaaa gccgcagccg ctaaagcgat catactgatt cgtttgccta gctgattcaa
    50161 tcggacctgt aaaggtgttt tttgcatagt attttcgttc aaaagatccg cgatcttacc
    50221 catttctgta gccatgccaa tggcagtgac gatcgctttc ccacgacctg cagcaaccgt
    50281 acatccttta aataccatat taagttggtc acctaatgac tcatcttctt cagcaataaa
    50341 tgctgcatct ttttcaactg cttcactttc tccagtcaac gccgattctt ctacacgtaa
    50401 ttgtgatgct tcgatcaaac gggcatcggc agaaatagca tcaccggact ctaaaacaag
    50461 aatatcaccc actaccagtt gctctgactc aacagtggtc actttgccgt ctcgtatgac
    50521 agttgccgtt tgtttattca tatcttgcaa ggattccagt gctttttctg cgtttccttc
    50581 ttggacaatc gccaataccg agttgatcac aacgatagcg atgatcagca gtccttcaaa
    50641 taaatcccca tgttctgtag caaatgcggt ataaaaggaa atcaccgctg ctacaagaag
    50701 gatgatcgtt gtaaaatcgg acaagcttcg aaagaatttt ttgatcatcg attctttcgg
    50761 cgcctcatcg aatttattca atccctgctt ttttcgccgt tcatccactt cttgtgaaga
    50821 caacccagta tctggatctg cttctgttac cttctttact tcttctattg tctgcttgta
    50881 ttcttccatc ctattcctcc tttaaaatct tcgtacaaaa aaaggtactt attttactat
    50941 aacatccttt ttttatttac ttcaaataaa gggcttatcg attatttgtt aagaaagcgt
    51001 aaaatttaaa gtgcttatat ttgcaaacgc taccaaagga tgctattata tagatgtaag
    51061 gaaggaggat accatcaaat agacagaaga atttctcgat agaacagtgt aagcaaacaa
    51121 agttgttaga agtacaggtt tcacccagac atgcgtgtag agtacaaacc tataaatagt
    51181 gaatgcgtat ggatgtttgg atcacgaagt atccgaaaca agaagtaatg cgatacgtaa
    51241 caattgaata acgtaaacgt ctgaaaattg aataggaaca agagatgttt caaatgttga
    51301 agatgtaacg aaaacaaaat ttttgtttaa catgaagaaa ttcaacaccg tgctttgcat
    51361 gtatgatgga aagttacaat catttctgtt tatttgaatc cttccgaggt acaagaaagc
    51421 cgagcttatc gagagctcgg ctttttcttt gtcataaaat ctttgtaaag cttttttgtt
    51481 attcaaaact agcggctttt cataacctta tttcacgaac atcgcatctc caaaactaaa
    51541 gaaacgatag cgctcttcaa tcgcatgctg gtaagcagat aaagtcaatt ctcttcctgc
    51601 aaaggcacta actaacatga ctaaagtcga ttttggcaaa tggaaattag ttgagaaagc
    51661 ttctaccact ttgaattcat agccaggcgt aatgaaaata tcagtccatc cgctgtctgc
    51721 tttgatttct ccattaaatt tcgttccaat cgtttctaat gttcgaattg aagttgttcc
    51781 gacagccacg attttcccgc catttttacg tacttggttc aatcgctcag ccgcttcttc
    51841 cgtcaaacgg tagaattcac tatgcatatg gtgttcttca attttatcta cgctgacagg
    51901 tcggaatgta cctaacccta catgtagtgt cagatagact aattcaacac cctttgcttc
    51961 gatctcatca agtaattctt tcgtaaaatg caatccagct gttggcgctg cagcagaacc
    52021 attttctttc gcatagacag tttgatagcg atctgggtct tccagacgtt ctttgatata
    52081 tggaggcaat ggcatttctc ccaatgattc aagaatctct agaaaaattc cttcatactc
    52141 aaagtcaatg atccgtccgc cgtgttccaa ttcttctttc actactgctt gcaatctgcc
    52201 atcaccaaaa gagattcttg tcccaacttt ggcacgtttc gcaggtttga tcaatgtctc
    52261 ccacgtatct ccttgtgtat tcgttagaag aaggacttct aaatgcgcac cagtatctgg
    52321 tttttctcca tataaacgag caggcagaac tcgtgtatta ttcataacta gcgcatcacc
    52381 aggctgtaac tcttcaatga tttcatggaa atgtttatct tccatctctc cagttttccg
    52441 atccaaaaca agtaaacgag aatgatcgcg gtcttttaat ggtgtttgtg cgattaattc
    52501 ttccggcaag tcaaaatcaa aatcttctgt tgttaacata tcgtcactca ctttctatca
    52561 tgtgcttcat cattctatca tgtttttcgt tctgttggca aaatcagcac tttttcactt
    52621 ctattttttg ttttcctaaa taaaaaaact accctatttg tattttttca ttgttttagg
    52681 catactagta atagatagtt cttcattgaa aatagaaaaa gaggtggcac catgattgaa
    52741 tttcaaaatg tgtcaaaaat ttataaaggt ggaaaagtcg ctgtagagga cgtcaatctt
    52801 tcatttgaaa agggtgagtt catttgtttt atcggtacga gcggaagcgg aaaaacgacg
    52861 acgatgcgga tgatcaaccg aatgactgat ccaacacaag ggaaaatttt gattgatgga
    52921 aaatccatcc aagaaattga tccagtggaa ttgcgacgtc agatcggcta tgttatccaa
    52981 agtattggat tgatgcctca catgacgatc cgtgaaaata tcactcttgt ccaaaaattg
    53041 ttgaaagtca gtcaagaaga acggaataaa acagctgaaa aaatgattga tcttgtggaa
    53101 ttgccccgtg agatgttaga tcgctaccca catgaattat ctggtggtca gcagcagcgt
    53161 attggagtcg ttcgtgccct tgcagcgaat caagatatca tcttgatgga cgagccattc
    53221 ggtgcgcttg atccaatcac tcgtgattcc ttgcaagacc ttgtcaaaga tttacaagag
    53281 cgtttgggaa aaacgatcgt atttgtaacc catgacatgg atgaagcgat taaactagca
    53341 agccgtatcg ccatcatgag tgaaggccgt gtgatccaat ttgatacacc agaaaatatt
    53401 ttacgtcatc ctgcaaacga tttcgtagaa gagttgattg gagaagatcg cctactacaa
    53461 gccaagccaa atgcgacaag agtcggcgaa gttatgctaa atacagcaat cacgattaca
    53521 ccggaaaaat cattacaaga agcaatcaaa ctgatgaggg aaaaacgtgt agatacgttg
    53581 ttagttaccg ataattcgaa tgtattaaaa ggatttatcg atgtagaaac gttgaataaa
    53641 aaacgaggaa aagtatccag tgtcggcgac attttgaata aacatgtttt ttacgtaaaa
    53701 gaatccgcct atcttcgtga taccttgcaa cgcattttaa aacgcgggct gaaatacgtg
    53761 ccggtcgttg atgaacagaa caaagtcgta ggtatcctga ctcgtgcatc attagtggat
    53821 atcgtctacg atgtgatttg gggagaagat cccgcaacta ctgatgttgc agagtcagct
    53881 actcctgaag aatctaagga ggtctgacct atgcagcagt tttttcatca gcacggttca
    53941 gaaattctct caaaaggttt tgaacatctt tatatttcag gacttgcttt gatcattggt
    54001 atcatcattg ctgtaccgct tggtgtctta ctgacacgtt ttccaaaagc tgccacgatt
    54061 gtgattggct taaccagtgc cttgcagaca atcccttctc ttgctttatt ggcattgatg
    54121 atcccgctat ttggcgtagg taaattacct gcgattatcg cactctttat ttattcatta
    54181 ttgccgattt tacgtaatac gtacattggg atgaaaaatg tcgattggaa ttatcgggat
    54241 gtggcaaaag gcatggggat gactgagttc caatccattt tttccgttga attgcccatt
    54301 gctatgccaa cgatcatggc tggtattcgt ttagctgccg tttacgtgat tgcatgggct
    54361 acacttgcct cttatatcgg cgctggtggt ttaggcgatt tgatttttag tggactgaat
    54421 aattaccaac cagatctcat tttggcaggt acgatccctg taacaatttt agcactattg
    54481 gctgattggc ttctaggtct cttggaacac cggctgacac cgatcgcact tagagaggag
    54541 aatgaagaat gaaacgtata ctaaaaatga ccatggttct tttctctctt cttatacttg
    54601 cgagttgttc cttccctggt cttgccagca atacagatga agacaccatt tcgatcacag
    54661 gtggaatcac ttcagaagca cagatcttag caagtctagt tgccggaatg gttgaacatt
    54721 atacagataa aaataccgca atcatcaaca atctggctac tacaactatc aaccatcaag
    54781 cgatgatgaa cggagatgct tcaatttcag cggctcgcta cacaggaaca gatttaacga
    54841 ctacattgaa ccttcctcct gaaaaagatc cgaaaaaagc gtttgctacc gtgaaagatg
    54901 aatttgagaa aagatacggt caaacgtggt tcccgtccta tagttttgaa aatacgtatg
    54961 tctttcttgt tcgaaaagac accgcacaaa aataccatct atctaaagtc agcgatctga
    55021 aaaatgtagc ggatgaatta gtagctggtg tagatacttc ttggatcaat cgtaaaggag
    55081 acggttatga cggcttccaa gaaacttatg gcttctcctt caattcgata ttacctatgc
    55141 agattggact tgtttatgac gcagtcgaag caggaaaaat ggatattgtt cttggctact
    55201 ctacggatgg acggatcgcc agttatgatc tagtcatgtt ggaagatgat cgtcaattct
    55261 tcccaccata tgatgctgct ccagttgtag acaacaagat attagctgat acgcctcatt
    55321 taaaagaagc cttaaataag ttaggtcata ccatcagtac ggaaaaaatg caggaattga
    55381 actacgaagc agataataat ctagtggaac catcagtagt agcacaacgt ttcttacaag
    55441 aaaaccacta ttttgaaggg aagtgaagca attggataaa atgaatgtct ttcaacaatt
    55501 tttctattat ttccaggaaa atggctccta tatctttgca caatttatcc gtcacttcct
    55561 gatttctatc tatggtgtcc tctttgcagc agttgtcggg attccggttg ggatcatgat
    55621 ttctaggaga cgtaaattag caaactgggt gattcgtttg gcgaatatca tccagacgat
    55681 cccttctcta gccatgatct ccattttaat cattggatta ggcctgggag tcaatgtcgt
    55741 gattgttact gtatttttgt attccctctt accgatcatc aagaatacgt ataccgggat
    55801 gatccaagta gacaaaaaca tccttgatgt cggcaaaggc atggggatga ctgctagaca
    55861 acgactattc atggtagagt taccactctc tgtttctgtt atcatggcag gcataagaaa
    55921 cgcgttagtt gttgccatcg ggataaccgc gatcggtgca ttcgtcggtg ctggtggtct
    55981 tggagatatc attatccgag gaaccaatgc aacagagggc acatcgatta ttttagcagg
    56041 tgcattgcca actgcattaa tggcgatcat cacggactgg cttttaggta ttttggaaag
    56101 acggctagat ccagccagcc gtacatcacg ctgatgttat tgtaaaaaac aaaagcttct
    56161 gcgaccattt gttggtcgca aaggcttttt tagttctgtc atttttaagg caataatcca
    56221 ctctgattct tccctcctta tgggcaaaga gttgcttttc ttagctattt atccattccg
    56281 tatactccaa cttcatactg ccaacgggtt catatctgca tccaccgact aagttttttt
    56341 cttatccgct aaggcatttt gccaatccat tttcccatca ctcgtcatgg aattcatacc
    56401 acatgatctc taaatactcg tctgcttgtt ctttgaattg ggcatcaatt cttacatcct
    56461 ttggcgtggc tttcatttaa gccttttcca tcagtcctcg attgttcgat gccttcagct
    56521 gcatcatcaa aactggtgac tgccgtatcg gtaacttcga tcatctttta tacaatcggg
    56581 aacttgccat cctcatcctt ggctaaaaac atatctagcc attccagctt ctcgccatgg
    56641 gaggtttgcg gttcctaaaa ccggtacacg attcttatgg actgcatccg tcgcaactag
    56701 acttggcgat acgatgatcc ttcaccagaa gagcaccccc gtaaaccaat tgatcgatat
    56761 attgccaata agaaaagaca ttggcgtaaa agatatcgat ccctcgtgat gcattgccgt
    56821 tgatactgct attcatggcc gaaagcgcca cgactttaac ttcttccgtt tgagtgacat
    56881 tcatgttcgg caatgtcgct ttggataccc gctctgctaa aggaacgaca ctggtattgt
    56941 atttcgtatc tgtatctttt ccaaaagacc atgccaacga atcctctggg aaccaataag
    57001 acgactccgg ttgattgtcc attgcttttt tcaacatact tttggctgtt tctcgataaa
    57061 cgacatttcc aattgtcttc gcctcacttt ggcgataatc gatggtcgtt gccactgcca
    57121 acactaacaa cgatgctcca attttactga ttttccttgt cacacttctt cctccttgtt
    57181 ttacataaac ccgtcaacat tccattccta aactcaaaca atgacccttc aaaggtggag
    57241 atataggaac aaattaaaga aatcatgcaa taaatccaag aaggttggtt ttagaacaaa
    57301 aaaagagtct cctttttggg gagactcttt gttaactatc taaaaaggtt ggctcttctc
    57361 acttcactca taatgtgtcc aggcagaata aatttctaca actttatttt cctcatcaac
    57421 tttgtaaacc actcgatgct gtctattcag cctgcgtgag tatctgcctt catgtgtagg
    57481 tctcagcttt tcaaaagact gcgtgggcat atatggatct tccttcaatg tctgaatcac
    57541 ttcttcaaat tgtttcttta aattcgtttg cttgattttt cttaaatcca ctttcgcaga
    57601 gtttttgatt gcgactgtat agttactcat caaagagctt cccaatcaat atcatcgata
    57661 ttcgtaaagc tactgtcgtc tttttcgcgg tctcgtactt tatctaaagt acctgtttgc
    57721 tcaagaaaaa gggtttcttg aatcgctctc cagtcttcca gcccaatcaa tacagcattg
    57781 tttccactac gatcactaat aatctcgatt tcagtatgat tttcatttac ctctttcaat
    57841 aactgataaa aatttttacg agcagtgcta ggattcatcg ttgtattagc cattgtctct
    57901 cctccttttt ctgatataat tatagcgtac ttgattaagt acgtcaaaaa aatacaataa
    57961 agcacttaaa ttcagcaaac ttttcctctt ttttacatag agaaaaagac ccctttttag
    58021 ggaggatctt tgtccacgct cacttcttac tcctttacga ctagcggtag ccaaacgtaa
    58081 agtaattggt aacaacgtat catctttagc aaaggtcgaa tccaaagcct gtgagtctat
    58141 gcaacaacac caatacacga aggttcctcg ttgcagtgtg acttgattga tatcttgaat
    58201 aacctgagga tacgcacgaa agatttgtaa cggttattca aaactgattt tggccgtcaa
    58261 gtcttctact gcctcaaaaa tgattgcctc tttcgtctcc tgtcattcat gttcactatc
    58321 gatttgatac ttgcttagac cgttgggttt gtgaatcacc aattaaaaat ggcactgagt
    58381 tatggtaaca aaaaatggtc acttgacgag gctgagattc ctacttatga agacacatgg
    58441 cacttaagag tcgaggtaaa ggaagaagct gaaaatatag gaggctgtac attatttggc
    58501 aaaggattcg gaaattatga tcaagacatc aaaatcaaaa tgttttacag ctaaatagga
    58561 agaataataa catttcatac gctgttccca tctttaatgg cttatagagg gcacctttag
    58621 ctgagaataa gacaaaacag accctctagc ttcaattaag agctagaggg tctgtttttg
    58681 attttaaaac ttggtttatt tcttcaaatg gtagctatag gtgctgacaa cgatattttc
    58741 acgccaagac aatcgcaatc ttaaccgcaa acttgtttgg ttatggagaa tattctgcca
    58801 gcgtctgttt ggtacaaatt gaggaatcaa cacagtagtt gtatagtttt gtttcgctgc
    58861 atttttactt acgatatcta cataacggat aattggattt tcaatcgagc gataagaaga
    58921 gtggacgatt gaaaaacgga catctgggaa atgcttttta aattctgctt cgatttcttt
    58981 ttctttttcg acatcttcgt ctagcgaaac atgcatcgca acgacataat caccgatcga
    59041 acgtgcgtaa ttcaacgcac cgatatttac ccgtgtcaca ttcccgacta atacaagaac
    59101 ggtattcccg tcataatcat gtagttttgc atcattttct aaacgaagct gttctgctac
    59161 ttttttataa tgatcatgga tcttataaaa aatgaacata acgattggca taatgatgaa
    59221 gaaaggccaa atgtccccta accgatagac aaacaaaatc gcaatgattg catatgagat
    59281 gaacgcaccc gtaatattgg caatcgattt gcttaaccat ctttttcctt cttttttcca
    59341 tttgacgacc atccctgttt gagataaagc aaatggaatg aacacgccga tagagtataa
    59401 cggaatcaag cgttctgttg agccatggaa aataaacagc aatacgatcg aacctagcgc
    59461 tagtgtgata atcccattgg aatagcctag tcgatcccca cgatcttgat acatgtgagg
    59521 catgaattta tcttttgcca gattgtatgc aagaactgga aaagcagaaa aaccggtgtt
    59581 agcagcaact gctaaaatca gcgctgtagc aaattgaagg acgtaataca aaataccatg
    59641 tccaaagaca gctttaccaa tttgagacaa aacagtgact tctttttcag gaacgatccc
    59701 ataccaatag ttgataaatg tgatccccac gaagaagaaa cctaagatca atgccatcat
    59761 cgtcaatgta gcagcggcat tttttgctct tggcttctta aagaatggta cagcattact
    59821 aatcgcctcc accccagtta aagaggaaga accagaagaa aatgctcgta aaatcaaagc
    59881 gatagaaaca cccggcacag ccgctcctgg aagtgcagta gcttgcaatg gcacagcacc
    59941 tgtcacgata ttatataaac caacaacgat cagtaacgag atcacaagga taaatgtgta
    60001 caccggaaat aataggaacg atgcagattc cctcagccca cgaagattca gcatcatcag
    60061 tagcaaaacg attgtcacag aaatagcgac ttgatgaccg tagagtgctg gcaccgctga
    60121 tgtgatcgct tcagcacctg ctgagacaga tactgctact gtcaacatgt agtcgatcaa
    60181 taaagagcca ccggaaatca agccagcatt ctttccaaga ttctcactgc tgacgacata
    60241 tgctcccccg ccatgaggat aagcatggat gatttgacga tatgatagcg ttaatgaaat
    60301 caataaaata ataacaaaag cggcaatggg tagcgaatac cagatggctg cagcagacaa
    60361 agcgactaaa acgacaacga tttgttccgt accatatgca atagatgata acgcatcaga
    60421 cgacaataat gccaaagcag caaatcgggt taatttgtgc tcatcatttt cagcagattt
    60481 taaaggctta cctactaaca acctttttaa gtaatccaat ttctaaactc ctttacacac
    60541 aattttactt caagttttat agtcgaaaat ttatagtaaa caagatgtta tagtacgctc
    60601 actgacctta aaagtcaata aactttaaga aaataattgg aaaaaaacag aaaaatatca
    60661 agcgcttttt caaacgcttt aatacagcaa aaatctaaca tttttttatg ggctttttct
    60721 ttgaattcta taataaaagg atagaacttg acttagaaaa tcaagttcta tcctttttga
    60781 tatcttaact tttggaagag agatgtaagg ctgctttcta cccatcattc ttactactcg
    60841 aagcatagcg cagttgtcac aacctcttga taaacggtgt ttaaaagctt gcatctgcgg
    60901 taataagccc aaccaaatta aaaacataag aagctgtttt tatttggtcg ttcttattac
    60961 ttgatgcaga gcagcttttt tacaacctct tggtacacgg tgttcaatca gctgaccctc
    61021 ggtattagct caaaaaaacg gcaaaatatg aggaactatt tttccatttt tttctctaat
    61081 actcaggtca aaacactgat ttcacaacct ctttttttaa tgaaaaatat ggctctcatg
    61141 cgccgcttct ttgaatttcg tcattttttc ttcgatgaac ggatagattg ccgttcctac
    61201 gattccaaag acaacaaaca aaccgatcat gatccagatt gctttcgtag catttggcca
    61261 ataaattcct cctgcagatt cccgtaacaa gtttaccgca tgagtaaatg gcaagaacgg
    61321 attgatcatt tggaagaatt tccctgagac ttggatcgga tagttcccgc ctccgccaga
    61381 gatcgacagt actaagatga taatagcgat ccctttaccg acattatcga ataatgcagc
    61441 caagacatag acgatcatca tgaacgctaa ggcgatcaag acagcaaatg acacgctgta
    61501 gaccggattt tggacatcta ctcctagtaa gaaaatattc cctaatgtaa cgatcaagct
    61561 ttgcgcaatc gccattgtca aaaatgtcag catacgagca acgaattgtt ctcgtttcgt
    61621 aaaattgttt cggtctttct ttcctaaata gtattctgtc gtagcaacac ttgaaagaag
    61681 aacagcgccg acccacagac atagtgcagt ataaaacggg gtgctagctg aaccgttatt
    61741 tgcgattgga taaagcgcat tggtttgtag ctcaacaggt tgtgtaaaga agtcactttc
    61801 ttttgttgcg tctagtttca ataatttgat gacttcgcct agatcgactt cttgttcgcc
    61861 ttttcggatc gcgtctgctg ctttttggat accagctttg atatttggcc agtcattgtt
    61921 gatcaagtcc gcagcagcgt tcacagctgt ttcgacttct ggcatttttt catttactgt
    61981 tgtcaatgta ccggtgattt cattttttag tgctggccaa tcattttgga taaaatcagc
    62041 ggccaatcct aatttttgct gaatgatcgg taattcattg ttgtataggt ctgccccttc
    62101 attgatgcca ttgacgatgg tatccatatt tccattcagc attgtattgg catcatggat
    62161 ttcttgtcca attgcaggca tttctttttg atatttttct aaaattgcca ctgcattcgt
    62221 tacagttgct ttcgttgagc tgagaagcga atcaaagtcg atctggtccg ctttgctcag
    62281 tgcgccttgt gcagtcccaa tcgtatcgat gacttggtct agtagagaag tgacagtgga
    62341 actgatttga cctgtattga taccagcaat gatgccattg atattattaa caacggcatc
    62401 aatttgatct agcaattcat caatagcctt cgtgtcttca ttttgaacag ctgtgttcaa
    62461 agcagatagg cggttttgca gattgcttag caagggtttt aattgattta gctgatcaat
    62521 aattggctgt aatttcgtat ttccagcact tgtttgcaat ttagtcagga actcgaccag
    62581 tttatcgatt gcattttgtt gttgagatat tccgtcaata aagccgttca atacattgct
    62641 gatgtttttc ttttcatcat cagtcagctt accatcttca atagctgttc ttacagtatc
    62701 tattaacgaa cctgtcgttg ttgccacttg tccaattgac ttcaatgtaa catctacact
    62761 gtttgtgatg cttggtatcg catctttcag ctgagaggca cctgattttg tttggtttgc
    62821 tagatcactt gcctgatttt ctaaattttt cacatctggc aagatatctt gtacttgttg
    62881 gatgatcgtc aagccttgtt ttgcttcatc gattccttgg ttcattgtct cttggatcga
    62941 agcaaaatcg ccgtcgatct ctgctagttg cttaccggca ttttggatct ctgggatttt
    63001 ttcttgcaaa tcaaggatga tttttgcttg atctttcaac tcaggcattt tgtcattcaa
    63061 tgcaacgact ttttcaccca tctcatctac tttagggatg taatccacga attcatttgc
    63121 tttattcagt ttttccttga tttcaggtag ttgactttgt aattcgagga cttgttttgt
    63181 gtagccgtcg atcgtgtcca aattttcatc tgttgataaa atcatatctt taactttatt
    63241 gatacttact aggttcgtat cgatgtcgta acctatctca ttgaatactt tcaataaagt
    63301 cgaactggct gttttgataa attcatttgt gatttgcgat tggatcgaac ttgctccttt
    63361 atcggtgatt ttcggtgcaa tcgcattgat tttttcattt accgtatatt cgatttttgg
    63421 tttcttgata tcaccactag taaaactcag cagatcttct gagaaatcct ttggcaagta
    63481 aatacctgcg taatactttc cggaacggac tccgtcttct aattgttctt ttgaatcaac
    63541 gaattgccat cctaattgtt tatttttatg aagcgattcg atgacttgct ccccaatggc
    63601 cacttcttta ccttgaaact ctgctggctt gtctgcactg taaacagcaa ttggcagctc
    63661 tccagtattt ccatatggat cccataatgc tttgatgtta aaccacgcat atagcgaagg
    63721 gatgatcatg atcgcgatga tcagaagtgt tgcaatcggg tttttgaaaa tccgtttcca
    63781 gtcaagcaaa tataatcgaa gcgtattttt gattgactgc atagttctcc cccttcttct
    63841 tttttcagct gaattacttt accatgtatt tcatactttt ttcaatcggc ttttttcaaa
    63901 aaagtcgaat ttttggacat atcacggcaa ttgtctcatt gtaatctctt acatattgag
    63961 aaataattaa ggattacagc ctttacttta tcatgaaata aaagggaaag ataattttat
    64021 gccaaagttt ttatctatta tacgtatacg caaaaaagct aaaacatttt ttgtctttgc
    64081 ccctacaaaa atgtttcagc ttttttcgtt ttttattcct gtatttctta tttttcgaat
    64141 gacaggatga ttttcccagc aggaggatgt ccttcaacta gctgatgtgc ttggatgaca
    64201 tgcttaagtg aagcagacag attcattgaa ataaaaacat gaaaggctcc tttttggtag
    64261 gctttgatta tgccagcaaa ggcttctgca tctagatatt cctttcttgg aacaaacgat
    64321 tcataaaagc cttccttttt ttgccgcaaa tctagatctg ggaggtcatt taatgcaaca
    64381 tacctgccgc ctggtttcat gatctggatg ccggtttctc ctttgatact tcctttcgtc
    64441 gcatcgatta caaggtctgc ctgatcagca aattgcagac ctggatttgt tttatcataa
    64501 gcagcaaatg ctgaagcttc taattttttt accttttcct catttttact ggatgcactg
    64561 gttaagattc ggattccctt ttcatggaga agctggatca gacttgaccc gaccgcacct
    64621 gatgctccta gtatcattac agtatctgtc ggctggattt ccgtcaaatg gttgattaga
    64681 ttataagccg taattcctgg cgtgaccatt ccggctgctt cttcccaact cattttatct
    64741 ggaatttttg ctacttttgc tgatggcaac acgacttctt ctccgtaagt cccgccgact
    64801 gcatggacag ctactcgatc acctacatga acagttgtga catctgaacc aatctctgtc
    64861 acaataccag cgccatcatt acctggtaca taagggaatt tcaatgttcg tatctctttc
    64921 atttctccta aacgcaaggc aacatcataa ggattcacac tgaatgcttt tatttctaca
    64981 cgaacatgtc cagaaccgac ttctctggga tgagcatcga tttcttcaaa aacatcttgt
    65041 gcttctccgt aaccgttaat tgcaaatctt cgcatggtca ccatctccct ttttctttaa
    65101 gtttacgttt atgcctttat tttgacaaaa aaaatgtctt ttcgttttgg cgcatgattc
    65161 aaaacaaaat agttgtcttt tttcgcgtaa cgtgttaaat tatttatcta aaagcgtaaa
    65221 gattacgatt tacgctaata aaaaataatt ggaggaattt gttgatggct aaaaaatcaa
    65281 agattgccaa agcaaaaaaa cagcaagaaa cgattgaacg ctatgctgct ttacgccagg
    65341 aattaaaagc gcaaaaagat tacgaaggct tgcgtaaact tcctttagat gctcgtccag
    65401 aacgattgaa aaatcgtgat ttaatcgatg gacgccctcg cggatacatg cgtaaattcg
    65461 ggatgtcccg tataaaattc cgtgaattag cacataaagg tttgattcca ggagtaaaaa
    65521 aagccagctg gtaatccaag tctggctgta atgtaaagga ggagggcgca tggaccactc
    65581 cttggatcat caaaaaagat acgttcgctt cttctagcag aaatgctggt actagaagca
    65641 gacgtatctt tttttacgca cagctggtcc ttttttatat gtaaaggctg catggttact
    65701 tcgctatatt tatttagtaa caggagtcag tgcttcgata cccacactaa ttttcaatgc
    65761 tcggtcaact tctgccataa tgggtcgatc caaatggcag accttttctt tcaatcttga
    65821 tttatcgatc gttctgatct gctccaacag gataacggag tctctttcta ttcctgttcc
    65881 agtagctttg atcccaatgt gagtcggaag cttaggttta gccatttttg ccgttatggc
    65941 cgcaacaatg atcgtcgggc taaaatggtt acctagatta ttttgaataa ccaaaactgg
    66001 gcgtgttccg ccttgctccg atcccacgac tggagaaaga tcagcaaaat aaatatctcc
    66061 tctttttacc ataggatcct ccttattctt gatattctct tggtatccgc tgtcctaatc
    66121 cgcaagccac ttcatagtgg atcgtaccta attgatctgc tatatcctgc atagtgattt
    66181 ctttgtctgc atttttgccg ataagcgtca cttttgttcc gacaggaaat tcttgaggca
    66241 atcggatcat cagttgatcc atacatacac gcccaatcgt ttcacagtag ttcccttcta
    66301 ccaatagaga aaaaccctgc atctttcgtg gccagccatc tgcataaccg atcggtatcg
    66361 ttccgatcca ctctgcttca ggagtgatat atgtttctcc atagccgatt ccttcacctg
    66421 ctgggagttt tttgacttga atcaattcag aaaccagctc aagtgctggc tgcaatggat
    66481 aaacttccgg tagtgcgtgc ccagagggat taagtccgta cattgctact ccataacgaa
    66541 tcatattgcc gattgtttca tcatgccata aagccgttgc actattgctg acatgtacat
    66601 atcgaggaag ttcgggaagt tttttcagca cttctataaa gcgttctttt tgcagattcc
    66661 aatatgtatc atctgcctga tctgctgtag aaaaatgggt aaataaacct tcccattcta
    66721 atacacgact ttctttgatg aatgcagctg cctcttttac ttcttcaggt gtacaaaatc
    66781 cgatacgacc catcccagtg tctgctttta gatgaatcga aagaggcgtc tctagcttac
    66841 ttttctctaa aacagcagct gctgcttgca accattcccg tgtacctgca gttactgata
    66901 aatcatgagc aacagctaca gtcagtgcgt cgtaaggaac catattcaag ataaggatgg
    66961 gctgagtaaa tccagcttct ctcaactcga ctccttcatc taaatttgaa acacaaaagc
    67021 ccgaagctcc acctgcaact gctgcctcag ctgtttcaat agctccatga ccgtatccgt
    67081 tcgcttttac tactgcaaac aattcttttc cttgcggaag tcttgcaact tcatttgcta
    67141 cattttcagt tatagctttt ttatgtatca ctgctttcgt tgggcggtgc caagcgacaa
    67201 ccatcgtttt tccttctttc tagtcttcca aaataatttg tgcaaaagct gtttcatttg
    67261 tatgagtaat actgacccat acattgcctt catgaggtga acgcgtcaca acaggctgac
    67321 cattttcatt ttttaaaatc tcgacatctt gaaaggtaac ttttccgatc ccagttcccc
    67381 aagctttaga gaaagcctct ttgcaagcgt aacgcccgcc taaaaattct acttggcgat
    67441 gaaaaggcaa ggactggaat agtttcattt catcagaggt caagattcgt gcaatgaatt
    67501 ttggtttttc ttcgatcagc cgagtgatcc taggaagttc gactgcatcg atcccaattc
    67561 cttttatcat atgcttttct cttttcaata aatttagaac actctcattc taccaaatta
    67621 taaccatttt gccacaaata agtccacaaa taacaaaaat gaaagtttct cttaaaaaag
    67681 agaaactttc attttatgtt taatcgttat ttgaacggat tacaaatccg cgttttttat
    67741 cattgtgacg tttaccgcca tctttttggc tgttattatt tttgctataa ccgccttttg
    67801 atttgtttcc acgataatta ccgccgtcac gacgattacg gttgtttccg ccaccacgtt
    67861 tgccattttt attgtatcct tttttctgca ttggcaatgg gcgttctggt gtgatcttca
    67921 ctggcactgc atcagctgga tcttttgctg tcgtttttag caaaagtgca actaaatctt
    67981 gtgcagaata ttcttctaat aatttatctg ctgtttgcag gtatttatct agaccatttt
    68041 ctgcaagttt tgtttcgatt tgttcaactg cagcacctaa ttgacctttg aatgcttctt
    68101 tttcggttgg tggacgtaaa gtcgtcatcc gttttttcgt caagttttca atcacatgca
    68161 agtagtccat ttcatttggt gtaacgaaag tcacagacat accgcctttt ccggcacggc
    68221 ctgtacgtcc gatacggtga acgtagcttt ctggatcttg agggatatca tagttgtaca
    68281 catgagtgac acctgaaata tctaatccac gtgcagcaac gtctgtcgct acaaggatat
    68341 ctaaatgacc gctcttaaat gaacgcaata cgctcattcg tttttgttga gacaaatcgc
    68401 catggattcc ttctgcacgg taaccgcgtg cttctaatcc gcgtgccaat tcgtctacac
    68461 ggcgttttgt acgtccgaag acgattgtta attcaggtgt ttgaacatca aacaagcgag
    68521 tcatgatatc gaatttttcg tattcttttg cgcggacata gtattgatcg atcaagtcag
    68581 ctgtcatttc tttcgctttg atcttcacat gatgtggatt tttcatgaat ttaacgccaa
    68641 tattcttgat agctggcggc atcgttgctg agaaaagtaa tgtttgtctt tgatcaggaa
    68701 cttgtgaaat gattttttcg atatcttcca agaagcccat attcaacatt tcatctgctt
    68761 catccaaaac taatgtttga acggttccaa gtttcaatgt gtggcggtta atgtggtcaa
    68821 gcatacgtcc tggtgtaccc acaacgatat gtggacgatc tttcaagcca cggatctgac
    68881 ggccaatatc tgctccgccg taaacggctt gtacacgtac ttttttgtcg cgccctaaac
    68941 gatataattc ttcttgtgtt tggatagcta attcacgtgt tggtgcgata actaaacctt
    69001 gtaattcgtg acgatctgga tcgatttttt ctaacattgg taatccaaat gcagctgttt
    69061 tccctgtacc tgtctgtgct tgtccaataa cgtcttttcc agctaatgct agtggaatcg
    69121 tttctgcttg gataggcgtt gcttcttcaa aaccagcgcg ttcgactgat ttcaacaatt
    69181 ctggtgataa ttctagttct ttaaatttca aatgaatcct cctatatggt tgttaactga
    69241 ggatgtagct tttttgctac cccgtcgatt tgttcggtca aagtgtattg tttctttttg
    69301 acagtggcca cgcacgtttt tatccattct tagcccctgt ggcaccaccg aaaaatcact
    69361 ttgcgaagtt taggcattag aaaagccaaa cgggtcgcgt tcagctttat cattattatg
    69421 acatctggct tcaacaagtt gtttctatgg caaaaccagt ccaatttctc ttcatctttc
    69481 ggcaataggt tattctaaca tagtttttcg ttttctgcaa gtttacagat ttttatttca
    69541 tttgctgaag ttgttctacc acttcaaata agcccatgct gttgctgcct tttagcataa
    69601 tactatctgt tgcttcaaga ttttgtttga tagcatcaat caatttattc ttttcttttt
    69661 caaaatagaa cacaggtagt tgggggtatt tttggttcaa ttcttctttt agtgcatgca
    69721 tttgttcccc ataaaggaaa atcatggcga atttctcagg atcgatatgt tcactcattt
    69781 gcgcatgcat atttaaagac tctttcccta attccagcat atctgctaac acagcgattc
    69841 tgcgtccttt tgttggcaga tttttaaaag tatctaatac cagtcccatg gctgttggat
    69901 ttgcattgta gacatcgctc aaaatagctg caccatttcc tgccgttagc cattcggttc
    69961 tgttttgagt gatagaaaca tgggcgagcc cttggcgtat ctcaggtatg gacagtccaa
    70021 agaagcgccc gatcgtatac gcaatcaaag cattcgttac attataactt ccaggcagcg
    70081 ggatcgtaaa tgtttctcct tctactacaa aatcggtttg tgcttttcct tttgctgtca
    70141 cttccgcatg aacatcgcca tttccaatac cgaatgttgt gactgtttgt actaatgttg
    70201 ccgttaaagg tgtcagcaac ggttcatctg cgggcacaac gagtaatccg tctgatacaa
    70261 gtccgtcagt gatttccatt ttggctttag cgattccttc tcgcgaccca agattttcta
    70321 tgtgtgcttc accgatcatt gtgattgctg cagcttttgg ttgagccata agtgaaagtt
    70381 ccgtgatttc attggcgtga tccatgccca tttccaaaac gatcttttct gtatcttggg
    70441 gcatgtgcaa aatcgtgtaa ggaagaccaa tattattgtt gtaatttcct tgcgtcttat
    70501 atgttttgta tttttcagac aagacagacg cggttagatc tttcgtggtg gttttaccat
    70561 tgcttcctgt aattgcaacg acttctgcgc ctactttttc caagtaatag gttgccaatt
    70621 gctgcatggc tttcaaagga tctgctacag gaataactgg aaaatttttt ggagcttgtt
    70681 caacaggcaa gctccaaaat gaagcagatg ctccgtttgt gatcgcttgc tggataaatt
    70741 catgtccatc tcgcgtacca gcaagaggaa cgaataaact tccttcggtt atcttccggc
    70801 tgtcaaattc gacattggta atctttgtca gtgcagagca ttcgctgccc acggcttgtg
    70861 caatttcttg tattgttaga ttcattgaga tctctcctaa aaaaggttgt gaaaaaagcg
    70921 tttggcctaa acggcgggtt cgcatgactc tcacttccaa gtaaatttct ctattcgtat
    70981 ttagaagcta gaatgaacgc ggttacgcaa tcactccagc ctcttcttta tttctcttta
    71041 ttcatttttt tcaaaaaaag attgacgctg atggtatcga ttcattccta actggatcag
    71101 ttcttcaatc aaatcaccgt atttcaagcc catattttcc cataagagtg ggtacatact
    71161 gaactccgta aatcctggca tagagtttaa ttcattcagg aataattcat ttttatttgt
    71221 caaaaagaaa tcgcaccggc tcaatccgct tccacctaac atcgtgtaag ctaacttcgc
    71281 gtactcttgc gctttttgat aaacttcttc tggcacttcg gctggaatct gcatttcgat
    71341 tttattattg atatattttg cttcataatc atagaatgct acgtctttta cgacttcacc
    71401 aggcaaagtc gtccgaacgt cttcatttcc taatacagca acttcgattt cgcgcgcttc
    71461 aattccttgt tcaacgattg ctcgagaatc atactgatag gctgttgcta aagcattttg
    71521 cagctcttct cggttttctg cttttgtaat gccgacacta gaacccatat tcgccggttt
    71581 gacaaacatc ggataaagca aagaaccttc acattgatca aatacttttt taggattttc
    71641 tttccattga ttcttaagta ctggtacata aggaacttgc ggcacaccag cagcttgtaa
    71701 aatatacttg gtcatgattt tatccatggc acatgcactg gtcaatacgc ctgcgccgac
    71761 ataaggcata ttcaatgtct ctaagaagcc ttggatcgtt ccatcttccc cgtttggccc
    71821 atgtaaaact ggaaaaacga tggctccttc ttctttgatt tcgcccggat tgatcacttt
    71881 tcctgtaaag ccttcctctg tctgtccact tgggtcccat gaaagatgca agacatcttt
    71941 gctggcaggt ttttctgtta gtaatggacc tttgacccat tgtccttctt ttgtaataaa
    72001 tacgagttga acttggtaat aattataata aatggcattt aaaactgaaa atgcggaaag
    72061 aatggacact tcatgctctg cgctgcgtcc gccatatagt aaagtaatct tcaaaagatt
    72121 gtcctcctgt attctaaatc gttcagtgat ttactctcat attttagcac aaaacaaaaa
    72181 taaacgaata acttatttat aaaaaaacga gccagcttct aaaagaacga gcgtcattta
    72241 ttttttgtca gctagattat ttgtcccgtt ttttataaaa agttatgata aagaaaagga
    72301 gggatgatga tggcgcaaac aaatccacgc agccgcttgg cagaaatcat tcgagtgttc
    72361 atcaattaca atgtagtgcc aaactttgta cagcaaaaaa atcccgaaca ggtaaaaaaa
    72421 gcctttgaag aactaggccc caccttcatc aagataggac agatgctttc tgttcgagaa
    72481 gatttgcttt cttctgcctt cactcaaaca ttcaaaacat tacaagatag tgtacctagc
    72541 gacacatttt caacggtcaa aaagacgatc gaaacagaac tttctctttc attatcagat
    72601 atattcgatg atttttcaaa atcgcctttt gcctctgctt ctatggggca ggctcatcgt
    72661 gccaagctga agaacggtga cgcggtagtc gttaagatcc aacaccctaa catcgccgaa
    72721 gagatccgac ttgatcttca actatttgaa cgagcgatcc ctttgatcaa atacattcct
    72781 gaaactagcg tagttgatct aaaaggggta ctccaagaag taaaacgttc cttgatcaat
    72841 gaaatggact tcttaaaaga aagtcaaaat ggcgaacaat tctatcaaaa gaacaatggc
    72901 tggaaagaga tccgcagccc caagatttat gacgcttttt gttcgaaaaa agtgatcgtg
    72961 atggaagaaa tgtctggtaa aaacttgaat catttaatga acgccgaaaa caaaactgaa
    73021 acttttatca ctggaattca aaacaagcag ctgaaacaag aagttgcaaa gcttttagtt
    73081 gagaacttta tgaaacaggt gtttgatgat ggttttttcc atgctgatcc tcatccaggg
    73141 aatctgctat tccatgtact aacaaaagaa gaacagacac aagctagccg aaaaactgaa
    73201 acagtccatg aaaaagagtt cggttccttt gcttttcgag cctccacttc tgcagaagac
    73261 cctgttgcgc catataccat caactacatc gattttggta tgatggggca tctttctgca
    73321 ggacttaggc aaaaactcac acaagccgtt ttagcgctgt atacgaaaga tgcttatcga
    73381 atcgaaaaag cggtccttcg actttgtcag caagaaggct cgtttgatga aagtcggttc
    73441 caccaagaac tgacaagttt tcttgaacag tattatgatt ctcctattga tgaaatcaac
    73501 ttgcaagaag tattcacaca tgtcgtgacg atttgtcatc aaaataatct gcagttcgat
    73561 cgtgatatca cgcttttgct gaaagcattt gggacattag aaggggtgat ccgcgtcctc
    73621 gatcctgaag tttccctcat ggaagtcgct tcgccttttg ctcaacatta ttttctcact
    73681 catctggatg tagaagatac attgaaacaa agcggattag acttattgga agggatgaag
    73741 gctgcgccta agatccctca acagctccac catctattag aaatgtggac ttcgggacaa
    73801 ggaaaagtca atttggaatt gaaaaagcaa gacaaattat tgagcaggat cgaaagtatg
    73861 atcaatcggc ttgttttcgg tatgatttta gctgcattga tcgtagggtc ctccctgctt
    73921 gtccaagcag cacctgtaga aaacgcagag gtcgtttcct tattagggat atttacgtat
    73981 gctattgccg cattcgtcat tatttttttg gcaatcgatg cattgatcca aatgtataag
    74041 aagaggaaaa agtagaaaaa ggttgtgaca gaaagaatca gcttcaagaa ataagaaggg
    74101 gttttacata aattgtttct caaatttttg tgaaaattcg gcttattttt cgaagaggct
    74161 acttctgaaa caccgagaat aaaaaatgtg aaaaagctgt tctgcaccca gtaataagaa
    74221 caatcaaaaa gggaacatgg aacacattta tcctgtttcc ctgttccctt tttgatgtat
    74281 tcgagcataa ataaaatacc ttcgttacct actatcttct gtacttggta aaccttagtc
    74341 aattgccttt tttaacgctt gacgaaaaga catatgatct ttctccgtaa agggttttgg
    74401 accctttgtc cttatgccag cttttcttaa ctttccacta tgataacgtt gttctagaag
    74461 cgtgccgata ttcgcttcgt caaattcttt tccagagtga tggaacactc gcacaccctt
    74521 cggcaataca agtgaagcta aaccataatc ttgcgtgatc aaaaagtcat tcttcttaac
    74581 cataccgacg attttataat ccgcactatc agctccgcgg tctacgtaga cgatttggac
    74641 atgattagcg tattctttgg tcgtataatg gtccacactg gtcacgataa ccacgtcaag
    74701 accataggat tctcctaatt ttatgacatc ttcttttact ggagaaccat ctccatcaat
    74761 gaatagcctc attctttttc gccttcttcc ataaatgata aacagttact aatccgctca
    74821 gtaaaagacc aacattgatc cattttgcca agataagcag ttgtacactt tctatcggca
    74881 ctgattcatg gttcagcgtg atcaataata gatggattgg ctctcccatt tcttttgcta
    74941 aaacaaacaa aagcgtcatc aataaaaact ggtggatcac actcccttta ttttgagtag
    75001 tatacttgac aacgatccgg cgatccattg gaatagctgt atcagtttga taatattcct
    75061 tatttagcca gctgcctgtt actacgtgcc agaaactaag cagaaagaca cctatcgcaa
    75121 aaccatttgg aatcaagagg accaataaag aagaactcag tccaagcaat tgcatagcca
    75181 atggagacca tgttaaacgt ttcaaaatcc atggtccgac tatttttgct gcaatcaagc
    75241 ctgctgcata aggcaaatac ataaaaatcg aaagtttttc taatccgtac agtatgccaa
    75301 cataaaaact gctaaataaa aaaaggaaat tccctagcat tccatttaga aaagtaaaca
    75361 gatttagcca gcttggcaac ttccattcct tcttctggtg tcctaaaaca aacacgaaca
    75421 agatgaacag cagcaagaaa ccataaatag caagatcaaa aagcctttca tcgaataaca
    75481 atcgggcatt tctcaaaaga aacaataagc caaagaatat caaaaataaa acaagctctc
    75541 gcgtagctac cagatttctt ttgatgtctt tgaaatccag ttcgtaacgc ggataatgac
    75601 taaccgtatg gtaggccatg acgtaaaaaa gtgtataaat agtaaagctt gctgcaatct
    75661 gagtcggtgc aggcaactca atcgcttgat acaggagaaa tagccagata gctgcaaata
    75721 agtacttttt cccatccata ttgataaaac cttgcgtctt ttcgtgaaaa ttgaccgtgg
    75781 tattagcagg tacaagccat gctgcactca gccctagcaa aacacttgaa aaataaaata
    75841 aaggaaaata aaatacgcct aatattccaa gcaatgaacc agcaccacca atcagcagag
    75901 cgatcatcag caacgtgtaa ctatctagac caaatcgtag actgttgact aaaaatagtc
    75961 cagtcatccg aaaggcataa aacagcgtaa atggcaaaat cgtcagccaa ttatgcgagt
    76021 ctgttagtcc caaaaataaa agataaggaa taaaaataac cgtgttactt aagataaagg
    76081 gcgttgtttg tgtaaaatta cgcccaaatc gtttgagttt ttcgaacatg acaatggctc
    76141 cttaaacagt cttataacta atctgctctt cgattttagc attaaagggg aaataaacca
    76201 attttacaga atatattctt gtaaaaaaaa atacagaaag aagcataagc ttctttctgc
    76261 atttttacgg tacttgatta ctcaagcaga caagcccttc tccacaaaag tcttgcccga
    76321 cacccctgca tccggcaggt gataaagata gaaatcaatt atcctgtgcc tccaaaatca
    76381 attaggagct ctgtaaacca gagtccttct cgacccgtcg cataaattta ctttaacaca
    76441 gttcctatgt ttatgcgaga gcttttttca ttttataatc gaccatttga aatccttgac
    76501 cgatatctga aataagttct tctgtaatct caaatccttc atgcttataa acctcgattg
    76561 cttgatgatt cccctgattc actcgcaaaa agatctctct ttcctttgtt tgcgcagcaa
    76621 gttcttcata ccagcgaaaa actgaagaag tcaatccttg accacgtact ttatttagca
    76681 aatataattt acttaaataa atttttccat ttatctcttc ataagcagtg taaccgacag
    76741 tcttttcttc gctttttaat aaaaagtagg acacaccttc tgtcagttct ttttgaatct
    76801 gtttttttga ctgataagtt ttcagcatgt aatccacttg tttttgacca atgatcggca
    76861 caaaaacttc cggccatact tcttgtatga ctgccatcag ctcatccagt tcattttctg
    76921 ttacttttat tttttccata cacccacgtc cttttttaca ttataaatga ccttgcgctt
    76981 gatggaataa aaagagcatg atcatgctat acacacccac cgaccacggt ttcaagaaaa
    77041 tcacaatctg cataaattcc ctaaaagata atttcgtcaa accagctact agacagatca
    77101 agtcgtctgg cgcaaaggga agcagcaagg taacaactag tactttttta atatctttcg
    77161 tttttcttgt cagcaattca tcaaatttct gatacttttt tggcgataaa ataagctgga
    77221 caaaggctac gccatagtaa cggacaagca aaaatccgat tgcttcccca atcactaggc
    77281 caatacaact atataaaagc ccaaatccat tgccaaacat cagcatgccc gctactgagg
    77341 agatccctcc cggcaaaata ggaatgatca cttggatcac ttgcaacaga acaaatatca
    77401 tcactgcata cttgccaaat tgctggataa acgcttgcaa agacgtgctc gatcgaaaaa
    77461 tcccatgatg ataagcataa agaatcaaca caaagaaaaa aaacagacca ataatgggca
    77521 tgatctgaag acatttttgt aaaactgatc cttttttaga catctatatt ccccacatct
    77581 tttttcattt attaaaactg cctcctt
//
LOCUS       DAHUVV010000004        70952 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.004, whole genome shotgun sequence.
ACCESSION   DAHUVV010000004 DAHUVV010000000
VERSION     DAHUVV010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 70952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.004"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(96..1097)
                     /gene="gap"
                     /locus_tag="NK767_000255"
     CDS             complement(96..1097)
                     /gene="gap"
                     /locus_tag="NK767_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCH6693278.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(1138..2229)
                     /locus_tag="NK767_000256"
     CDS             complement(1138..2229)
                     /locus_tag="NK767_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HCH6693279.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(2378..3712)
                     /gene="rpoN"
                     /locus_tag="NK767_000257"
     CDS             complement(2378..3712)
                     /gene="rpoN"
                     /locus_tag="NK767_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCH6693280.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            3772..3843
                     /locus_tag="NK767_000258"
     tRNA            3772..3843
                     /locus_tag="NK767_000258"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3805..3807,aa:Arg,seq:ccg)
     gene            complement(4035..4919)
                     /locus_tag="NK767_000259"
     CDS             complement(4035..4919)
                     /locus_tag="NK767_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCH6693281.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(5006..5905)
                     /locus_tag="NK767_000260"
     CDS             complement(5006..5905)
                     /locus_tag="NK767_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HCH6693282.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(5922..6428)
                     /locus_tag="NK767_000261"
     CDS             complement(5922..6428)
                     /locus_tag="NK767_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCH6693283.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(6617..7237)
                     /locus_tag="NK767_000262"
     CDS             complement(6617..7237)
                     /locus_tag="NK767_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCH6693284.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(7313..7750)
                     /locus_tag="NK767_000263"
     CDS             complement(7313..7750)
                     /locus_tag="NK767_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCH6693285.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            7913..8743
                     /locus_tag="NK767_000264"
     CDS             7913..8743
                     /locus_tag="NK767_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCH6693286.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            8727..10094
                     /locus_tag="NK767_000265"
     CDS             8727..10094
                     /locus_tag="NK767_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCH6693287.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            10113..10922
                     /gene="yidA"
                     /locus_tag="NK767_000266"
     CDS             10113..10922
                     /gene="yidA"
                     /locus_tag="NK767_000266"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCH6693288.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(10965..11408)
                     /locus_tag="NK767_000267"
     CDS             complement(10965..11408)
                     /locus_tag="NK767_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCH6693289.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            11542..11913
                     /locus_tag="NK767_000268"
     CDS             11542..11913
                     /locus_tag="NK767_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCH6693290.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(12082..12864)
                     /locus_tag="NK767_000269"
     CDS             complement(12082..12864)
                     /locus_tag="NK767_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HCH6693291.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(12861..13832)
                     /locus_tag="NK767_000270"
     CDS             complement(12861..13832)
                     /locus_tag="NK767_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCH6693292.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            13924..15318
                     /locus_tag="NK767_000271"
     CDS             13924..15318
                     /locus_tag="NK767_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCH6693293.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            complement(15510..16865)
                     /locus_tag="NK767_000272"
     CDS             complement(15510..16865)
                     /locus_tag="NK767_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HCH6693294.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(16895..17530)
                     /locus_tag="NK767_000273"
     CDS             complement(16895..17530)
                     /locus_tag="NK767_000273"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HCH6693295.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            complement(17537..18913)
                     /locus_tag="NK767_000274"
     CDS             complement(17537..18913)
                     /locus_tag="NK767_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HCH6693296.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            complement(18906..20687)
                     /locus_tag="NK767_000275"
     CDS             complement(18906..20687)
                     /locus_tag="NK767_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HCH6693297.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            complement(20709..21020)
                     /locus_tag="NK767_000276"
     CDS             complement(20709..21020)
                     /locus_tag="NK767_000276"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HCH6693298.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            complement(21010..21996)
                     /locus_tag="NK767_000277"
     CDS             complement(21010..21996)
                     /locus_tag="NK767_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HCH6693299.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            complement(22011..22595)
                     /locus_tag="NK767_000278"
     CDS             complement(22011..22595)
                     /locus_tag="NK767_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HCH6693300.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            complement(22616..23086)
                     /locus_tag="NK767_000279"
     CDS             complement(22616..23086)
                     /locus_tag="NK767_000279"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HCH6693301.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            complement(23105..25108)
                     /locus_tag="NK767_000280"
     CDS             complement(23105..25108)
                     /locus_tag="NK767_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HCH6693302.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            complement(25095..25427)
                     /locus_tag="NK767_000281"
     CDS             complement(25095..25427)
                     /locus_tag="NK767_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693303.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25764..26483
                     /locus_tag="NK767_000282"
     CDS             25764..26483
                     /locus_tag="NK767_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HCH6693304.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            complement(26813..28711)
                     /locus_tag="NK767_000283"
     CDS             complement(26813..28711)
                     /locus_tag="NK767_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HCH6693305.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(28872..30134)
                     /locus_tag="NK767_000284"
     CDS             complement(28872..30134)
                     /locus_tag="NK767_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6693306.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(30127..31026)
                     /locus_tag="NK767_000285"
     CDS             complement(30127..31026)
                     /locus_tag="NK767_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6693307.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(31171..32949)
                     /gene="uvrC"
                     /locus_tag="NK767_000286"
     CDS             complement(31171..32949)
                     /gene="uvrC"
                     /locus_tag="NK767_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCH6693308.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(33131..33445)
                     /gene="trxA"
                     /locus_tag="NK767_000287"
     CDS             complement(33131..33445)
                     /gene="trxA"
                     /locus_tag="NK767_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCH6693309.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(33532..35892)
                     /locus_tag="NK767_000288"
     CDS             complement(33532..35892)
                     /locus_tag="NK767_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCH6693310.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(36007..36555)
                     /locus_tag="NK767_000289"
     CDS             complement(36007..36555)
                     /locus_tag="NK767_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCH6693311.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(36613..37050)
                     /gene="zapA"
                     /locus_tag="NK767_000290"
     CDS             complement(36613..37050)
                     /gene="zapA"
                     /locus_tag="NK767_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCH6693312.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            37252..38169
                     /locus_tag="NK767_000291"
     CDS             37252..38169
                     /locus_tag="NK767_000291"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCH6693313.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(38547..39458)
                     /locus_tag="NK767_000292"
     CDS             complement(38547..39458)
                     /locus_tag="NK767_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCH6693314.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(39859..40437)
                     /locus_tag="NK767_000293"
     CDS             complement(39859..40437)
                     /locus_tag="NK767_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCH6693315.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(40457..41125)
                     /locus_tag="NK767_000294"
     CDS             complement(40457..41125)
                     /locus_tag="NK767_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6693316.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(41140..42210)
                     /locus_tag="NK767_000295"
     CDS             complement(41140..42210)
                     /locus_tag="NK767_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6693317.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(42381..43745)
                     /locus_tag="NK767_000296"
     CDS             complement(42381..43745)
                     /locus_tag="NK767_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCH6693318.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(43742..45301)
                     /locus_tag="NK767_000297"
     CDS             complement(43742..45301)
                     /locus_tag="NK767_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6693319.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(45357..47837)
                     /locus_tag="NK767_000298"
     CDS             complement(45357..47837)
                     /locus_tag="NK767_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693320.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(47821..48156)
                     /locus_tag="NK767_000299"
     CDS             complement(47821..48156)
                     /locus_tag="NK767_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693321.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(48170..49204)
                     /locus_tag="NK767_000300"
     CDS             complement(48170..49204)
                     /locus_tag="NK767_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCH6693322.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(49294..49974)
                     /locus_tag="NK767_000301"
     CDS             complement(49294..49974)
                     /locus_tag="NK767_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCH6693323.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(50144..51103)
                     /locus_tag="NK767_000302"
     CDS             complement(50144..51103)
                     /locus_tag="NK767_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCH6693324.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            51347..52798
                     /locus_tag="NK767_000303"
     CDS             51347..52798
                     /locus_tag="NK767_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCH6693325.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            53147..53779
                     /locus_tag="NK767_000304"
     CDS             53147..53779
                     /locus_tag="NK767_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCH6693326.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            54367..55806
                     /locus_tag="NK767_000305"
     CDS             54367..55806
                     /locus_tag="NK767_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6693327.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            56101..56319
                     /locus_tag="NK767_000306"
                     /pseudo
     CDS             56101..56319
                     /locus_tag="NK767_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            56568..56783
                     /locus_tag="NK767_000307"
     CDS             56568..56783
                     /locus_tag="NK767_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693328.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(57000..57256)
                     /locus_tag="NK767_000308"
                     /pseudo
     CDS             complement(57000..57256)
                     /locus_tag="NK767_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(57443..58354)
                     /locus_tag="NK767_000309"
     CDS             complement(57443..58354)
                     /locus_tag="NK767_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCH6693329.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(58373..59377)
                     /locus_tag="NK767_000310"
     CDS             complement(58373..59377)
                     /locus_tag="NK767_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCH6693330.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(59374..61497)
                     /locus_tag="NK767_000311"
     CDS             complement(59374..61497)
                     /locus_tag="NK767_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCH6693331.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(61502..63949)
                     /locus_tag="NK767_000312"
     CDS             complement(61502..63949)
                     /locus_tag="NK767_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCH6693332.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(63936..64325)
                     /locus_tag="NK767_000313"
     CDS             complement(63936..64325)
                     /locus_tag="NK767_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCH6693333.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(64385..64888)
                     /locus_tag="NK767_000314"
     CDS             complement(64385..64888)
                     /locus_tag="NK767_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCH6693334.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(64901..65125)
                     /locus_tag="NK767_000315"
     CDS             complement(64901..65125)
                     /locus_tag="NK767_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693335.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(65229..67139)
                     /gene="ltrA"
                     /locus_tag="NK767_000316"
     CDS             complement(65229..67139)
                     /gene="ltrA"
                     /locus_tag="NK767_000316"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCH6693336.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(67885..68022)
                     /locus_tag="NK767_000317"
     CDS             complement(67885..68022)
                     /locus_tag="NK767_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCH6693337.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(68019..69203)
                     /locus_tag="NK767_000318"
     CDS             complement(68019..69203)
                     /locus_tag="NK767_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCH6693338.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(69462..69752)
                     /locus_tag="NK767_000319"
     CDS             complement(69462..69752)
                     /locus_tag="NK767_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693339.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(69742..70884)
                     /gene="dcm"
                     /locus_tag="NK767_000320"
                     /pseudo
     CDS             complement(69742..70884)
                     /gene="dcm"
                     /locus_tag="NK767_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
BASE COUNT        20403 a        14825 c        12378 g        23346 t
ORIGIN      
        1 aataaactta ttatcaaaaa aagcggaaga agcgcgattg cttccccgct tttaaatgta
       61 catgtctata atttacactt gtacgtttgt ctatcttata agttagcgaa gtattctaaa
      121 gtacgtacta attgtgcagt gtatgacatt tcgttgtcgt accaagcaac agttttaact
      181 aattgtttgt cgccaactgt cattacttta gtttgtgtag catcaaataa tgaaccgtaa
      241 gtcataccaa cgatatctga agaaacgatt tcgtctgtgt tgtatccgta agattcgttt
      301 gaagcttctt tcattgctgc attgatttcg tcaacagtta cttctttttc aagaactgtt
      361 actaattctg ttaatgaacc tgttggtaca ggaacacgtt gagcagctcc gtctaattta
      421 ccgttcaatt caggaattac taaaccgata gctttagcag cacctgttga gttaggaacg
      481 atgtttgcag cagcagcgcg tgcacggcgg aagtcacctt taggatgagg tccgtctaga
      541 gtcatttggt cacctgtgta agcgtggata gttgtcatta atccttcaac aacaccaaat
      601 ttgtcgttca atgttttagc cataggagct aaacagttag tagtacatga agcacctgag
      661 ataactgttt ctttaccagt taatgtttcg tggtttgtgt tataaacgat tgttggaaca
      721 tcgtttccgc caggagcaga gataacaacg cgtttagcac cagctgttaa atgtttttca
      781 gctgctgatt tagaagtgaa gaaaccagta cattctagaa cgatatctac gcctagttcg
      841 ccccatggta attcttctgg gttgcggtta gctaatactt taacttcttt tccgtttacg
      901 ttgaatgaac cttcatgaac ttcaactgtt ccgttgaaac gtccttgagt tgtgtcgtat
      961 tttaacaagt gtgccaacat tttagcatct gtcaagtcgt tgattgctac tacttcgatt
     1021 ccatctacat cttggatacg acggaatgct agacgtccaa tacgtccaaa accattaata
     1081 cctactttaa ctgtcattta agatttcctc cttatgaaaa tcaaaaaaat atttatttta
     1141 aagggttacc ccttttaaaa tctcatttgc agcagcttca tcagtgatga gccacgtttg
     1201 ttttggtgca tttttcatat aagctcgaat tgctttggct tttgattttc cgcccgcgat
     1261 tgccatgatg atcgggactt tttccaaatc ttgcaattct atgccgattc tagggacctt
     1321 ataaacgact tcaccttttt cattgaaaaa gtagccaaaa gattccgcaa cagcatttgc
     1381 ttgtttcagc atgactaatt ctttttcagt catcttacga cgtgctgcca tgtgtaatgc
     1441 acgcccaata ctatgaataa cacaattcgc ttcactaatc aagtttaaga cttcttggat
     1501 agatggctca ttgagtaaag aattgtaagt tgccagactt aattgttccg gaacataaag
     1561 tgcacggtgg ttaccgtttg ctttagcagc cattcttgca ctgacagaat ttgcctgtac
     1621 agttatcgct tcaccaatcc cgcctcttgc agggacaaac aggttatgcc gtttcttatt
     1681 ttccagatta gacaattgtt cggctaccac agccatagta gttcctccca tcacagcgat
     1741 gatattctcg ccttctggaa gtagtcggtc tagggcttcg ctcagcgctt cgccgaatgc
     1801 atcagccact ttggtctgct cttcgctgtt tcctgaaaca acgatacagc gttggttacc
     1861 aaaatattct gccaactgtt gttcggtttg atgcgtacct aaaagctgat ccatgaagtt
     1921 ttcaaggctt tgataaactt cttcgccctt agttgtcagc gtcataccgc tttttgaggt
     1981 gtcgatcaaa ttcagatttt tcaaaagatc tgtttctgtt cgcaatactc gttctgtgat
     2041 ccccatactt tccgataaac ttcgacgtcc aatcggctgc atccaataga tatttcgtaa
     2101 aattttatag cgttcttgca acacttcgat aatatctggt gcaacagatt caatcatttt
     2161 tatttcatcc agcatagcga tctccttagt tggacacttt acgtccaatg tagacttaaa
     2221 acgacccata tacgataaaa aaagaaatta actattcgaa tttcacatca ataaagggaa
     2281 atctcaacct cacgactgta agtctaacag ttttgctctc gtttttcaac taaaaagact
     2341 agaaaaagtg aaatactttt tctagtcttt tgactgatta gttatcataa cgttttcttt
     2401 ttgatgaact cgggattcct aataaatcac gatattttgc tactgttcgt cttgagatcg
     2461 ctatctcatc ttttttcagc aattcaacca atttctggtc agacaacggc ttgtttttgt
     2521 cttcttgatc gactaattcc tgcagctttt tcttcgctgt atctgctgaa aggtcttctg
     2581 tctgttctgt actattcgta ggaattcgtg tagtgaaaaa tttttttaat tcaaatacac
     2641 cgaagtctgt ttccaaatat ttcccgttga ctgcacgact gaccgtggat tcatgcacat
     2701 ctatctcagt tgcaacttcc tttaacgtaa gaggcttgat tggacgttcc ttgtcgataa
     2761 agaaagcttt ttgccgggaa acaatgactt gagcaacccg caaaattgta tccccacgtt
     2821 gtaggatcgt tttcttcaac cattcgaatt cctgtttccg ttcttttaaa taattttcag
     2881 tttctttatc tgcttgttgt gacatttgtt tgaagtaact ctcttgaaag cgtatatttg
     2941 gctgattttt tcggtttgag ataacttgta catttttatc gtcgtccact aatacccgga
     3001 catcaggaat aatgtacaat tcgctgctac ggtcgaaaat ccggccaggg cttggactca
     3061 acgtttgtaa atagtcgaat atctgctgaa cagcatgtag atcaacagaa aaacgttgtg
     3121 cgatttcttt ccattttcgc tccaccagtt cctcaaaaca ttcttccaac acgatataag
     3181 ctaattccgg cgcataatca tctcgttctg tttgaagcat gagacattct tgcaaactac
     3241 gtgcacctac tccagctggt tctaattgct gaatcagagt caacgcatcg agcatttgga
     3301 taggcgtagc tccaatctct tttccggctt cttctaaact aatcgtcaaa aatccgttca
     3361 aatcgatgta ttctacaaga taaagaacga ttttcctcaa aaaagtatct cggtaattca
     3421 aatggacttg ttcgatcaat gaatcaaata aagacccttt tgtatcagga atctgattta
     3481 ggtaatttgt ctcttctcct tcataagaag atgaagaagc ttttatgtaa tctggctgcc
     3541 attccggctc aacaacttcc accaatggat tttccattgc ttgattctca acgaatgcca
     3601 acagctcctc actgttatat tgtaaaactt ggatggactg ttgcaactgt tgcgtcatgg
     3661 ctagtttctg tgtctgctgc tgtttttgtg aaaaattttg ctcaaatttc atttttttgt
     3721 ccaatcccct tgtaattttt tatctgttta gttacaatta tcccagtgct tgcgtcctta
     3781 gtgtagtgga tatcacgtaa gattccggtt cttgagacgg gggttcgatt ccctcaggac
     3841 gcgttctact gctattttag cataatttac gaatagttgc ttctttattt ttaagtgaaa
     3901 actgtccgcc aaagaaaatt ggtggacagt tttttgttgt acctgtatag atattgttaa
     3961 gcctttgcaa gtaaggtagt tatgtgttca actacttatt aaaatgtgtt atttattaaa
     4021 gatacttgtc tcacttactg aactggccga tgatcgtttc tgccataata gttctcgaca
     4081 gccgttggat gccctaataa atccccaact gtcgtgatgc gatacccttg ctctttcaga
     4141 taatcgatca cttgaggaac ggcacgaatc gtttcaggat agatatcatg gaataaaata
     4201 attgccccat cgtatactgt tgcttggatc ttctgcaaaa tcaaatcagg atttcttgtc
     4261 ttccaatctt ctgaatcaac agaccattcg atgatcggtc gctggatgat cgttgctcct
     4321 acattcgtga cagaaccata gggcggacga acaaaagtgg gggttttgcc agtgactttt
     4381 ttgatctcag tgtctgcttt cgtcacttcc tccgtcatcg actgggcaga aagagtggcc
     4441 agattctgat gatcgtatgt gtgattgcca atctcgtgcc ctgcttcagc tgtctgcttc
     4501 acgatttcag gatgctgcgc cgtattttct cctaagacga aaaaagtggc atgcacgttt
     4561 ttttctttta agattcgcag aagctgcggt gttgtggtcg ctcctggtcc atcatcaaag
     4621 ctgattgcga cgcttttgac agccggatct cggctcgttt ttgtactgtc ttgtgttgat
     4681 tgtgatgact gactttgtac cttttccgct gattcttgaa tggcttcttt gacgttagct
     4741 gtttcagttg attggctttt aatggttgat tgttctgatt gtttgaaaga ttgtgacgaa
     4801 tgctccgaag agtgtgtttg tccttttact tcggtgatca aggaggccac cgaagtaatt
     4861 tcgaaaaaca aaagcacgga taaagcaata atactgataa ctaaataatt tcttttcact
     4921 atatctcttc tttctctttt tctcttctta ttatttactt gtacttactt gaaaattgaa
     4981 actgctagtc gcaactactt ctcgtctaat gatccaaata atcttccgat gccaaaatcg
     5041 tttttgtcac ggacttagga atgacaaatt ctggtgtacc catataacca ggagcaattt
     5101 cgtattgaga aaaagtcaaa actaattgat gctctttgtt caaataaaaa cttcgtgtag
     5161 gcgtaacaag tgttggtttt tctatcgtcc cttcttgaat atcttgctca gtccaatagt
     5221 acttagatgg atcttccttg acttgttcgg ccatctgccg tttgacttct tttgtcaaaa
     5281 cagatagata ggtgtcatct ttgaataaaa gtggaagtga gagctggaca ctgtttttct
     5341 tgtcgattgt gtcgtatctt ttttcaacat ggctatctgc tgctgtttgg gtgatctgcc
     5401 tttcaatcac gagaaaccgg ttatcgtcta cgattttctg ataatctccc aaaacttggt
     5461 aatgatccgt ttcaaaattt ccatattctt tttcagcttt ttcaaaatct gcttgtcctt
     5521 cgcgaaagta tttttgattt aatccatgaa ttgttttatt ttctttctca ttttgtgtat
     5581 tgatcttcgg tacctgaata tcgatttttt cagatcgatc agtaaagcgt tcgcctaaga
     5641 tcaattcaat gactgatcct aagatgggta aatcttctgc aaagcttcga acttgactat
     5701 taaaaagaag gctacttgtt gtaatcatta cgatagccaa agatactccg aacccttgga
     5761 gacgtctctt tctccgcttt gcttttataa atgattgctc ttcgataata aaacgctggc
     5821 ggatctcctc tttaagccct ttgggaattg cttgttcttt ataagttttc tttagttttt
     5881 ttaaattttc tttttgggaa tacatggcac tcttctctcc ttcattctag gatttgacgt
     5941 aaagcattca atgagcggta cattttcgtt ttaactgtgt tttcattcat atccaagata
     6001 gtcgcaattt ctttcaaacg aaatccttca aaaaagtaga ggatcagaat ctctttttcc
     6061 ggactgtcta ttttatctaa aatcatatgg agctctgcaa cggaagtttc taaaggtaac
     6121 tgttggggga tatcttgaat ttcaaataag tccacaggcg tccttttttg tctgcgccaa
     6181 ctatctaatg ccgtgcggat caatatccga taaaaccatg cactaaaatg ctcgatctct
     6241 ttttcttttt caaaatagtt cagggcttta tgaaaactat cttgcatcac atctaaggcc
     6301 tcttctttat catgtacgta attcatcgtg atacggaaca ttttttgata atcagtttcg
     6361 atgagtccat tcagcaggtg acggtatttt tcgatctgcc gttttttctt atatccagta
     6421 aaaatcattc tccactcctt tctgcctcac ttaatatgac gtacaaggtg cgctaaaagt
     6481 ttcaattttt ttatttttta tgtatttact tgggattttt atatacaaaa aagaaggtgt
     6541 gacaaaaatc gtgtcacacc tccttattct gataggctga tcgtctctgt cacaacctct
     6601 cgtaagaaaa taatgtttat tctttttcag agtcagaaaa ttcttcctct tcataatctt
     6661 catcatcatc gtcttcgtct atgatactta aatcttcttc gatatttcct ggcaagtctt
     6721 cttcatcatc tgtcgggtca tctgctccga tttcttgcaa gtcagaatta taagcagcga
     6781 tttcttcatc ttcatcatca tcgtcatcaa acagatcatc gtcatcttct tctgtcagat
     6841 ctgtatcttc tgggtcatcg tcattgtaat cgatcgcgtc ttcatcatca ttgataaagg
     6901 cgttgacttt cttgcgttta cggcgacgtg gagtatcttc atcctcttcg tccattccat
     6961 ggtttacttc ttcatcgatc gaatcgatag cataccatga acgcaagccc caacgattat
     7021 cccctaatga gataaagctg ccgtcaatat tcaagtcagt ataaaattgt gcgagtgaat
     7081 cacgaatttc actgtctgat ttcccaagat agttttggat ctggttagct aaatcagaaa
     7141 aatccatgac atcgccacgt tgttctaaga tcgcgtgtgc tacttcgatc attgataatt
     7201 cgcttttgtt tgcaccttca aatacactaa tttccaatca ggacacgtcc tttcacagtc
     7261 tactccttat catacaaaat cagacacaaa aaatcaaact tttcttttat atttacgctg
     7321 taaaatccaa ggtcaaataa tattcgccga ttttttccgg tcccatgaac agatcataat
     7381 cgattcgaac cttccctgct gtgggacggt ctttcaaact aacatgcagt tctttcgtaa
     7441 aagtagcaat ctggatcata ccgtatggtg ttcgatagga tgtttctaac cgttctttat
     7501 agccaaattt caaacgcatc cgcatttctc ctgcacggat cagctgtaca tgaccatctg
     7561 gaactaattt gatcgttaca ggaatctcat cgcccgtctc ctcctgaatc tctttataac
     7621 gtatgtacag cgtatcgcca attgtcacca tttgcccttt caaatcaaaa taaaaatctt
     7681 gcacttcccc ttgctgtttt acttttgtac gcaatttgat tgaaatcggt gttccttgtg
     7741 ataattccat tattcattct cctttacttc tcatagctac attgttccta gtttcttttg
     7801 ttttgtcaac caacttgtcc atttttacag aaaacctttt ttccaaaaca actctttgat
     7861 tttagagtat aatattgtag aaaatatgaa tttatacaaa ggagccggtt gaatgaaccc
     7921 tgcgattcga ctagatcagc acatttatca aaaaaacgac ttttcccctt ttgcattaac
     7981 agatatatta acggaatatt ctaaagaaca ccagcaaatc tttttacatt tctggcaata
     8041 tgactgcaca gtcatcttag gtatgaagga tgttcgaact ccttttctaa gtgacgggat
     8101 ccaaacactg gaagatgcag gttacgctcc agtgatcaga aattcaggtg gattagggat
     8161 cgtatcagat gaaggaattt tgaatatttc tttgattttc ccccaaagtg acacaaaaaa
     8221 aataaccata gatgacgctt acgagcaaat gctcctattg actaaatacg cttttccaaa
     8281 ggcaccgata caagcattcg aaatcaccca ttcttattgt cctggaactt atgatttaag
     8341 catccatggc aaaaaatttg ccggtatcgc tcagcgtcgg atcaaaaatg ggatttctgt
     8401 tatgatgtac ttaagtgtca acggtaatca ggaaaaacgt gggcaagtca tgaagcgatt
     8461 ttatcaatct gctttaaaag aagactttgg tacaaatggc tatcctgcag tggatcctgc
     8521 tacaatggca acacttgaag aactgcttga ccaacccctt tttgtaaaag aagtcaaaga
     8581 agcatttgca aaagcttttg aaaagctgta ttctgcaagt gatttacagg attctaccga
     8641 ctggctaaaa aaagaaatcc agctggagca atggaacaac caaatccaga gaatggaaca
     8701 acgtaataac ataaaggagc tacattatga caacccctta taaatatcaa atgctgccta
     8761 tggaaaaagt ttttcgtgat ccagtccata actatatcca tgtccagcat caagtaatct
     8821 tagatttgat caattcgaaa gaagtccaac gtctacgacg cattaaacaa ttaggcactt
     8881 cttctttcac ttttcacgga gcagaacata gccgttttac tcattcatta ggcgtttatg
     8941 agatttcaag acgaatctgc gatatctttt ctcgaaattt ttcaaaagaa aaaataggca
     9001 acggaggctg ggatgatcag gaacggctgg tgactttatg tgcggcactg ttgcatgatg
     9061 tcggtcatgg tccttactct catacatttg aacatatctt ccatacgaat catgaagcca
     9121 tcactgtaga gatcatcact tctccagaaa cagaagtgta tcagattctt aaccgagtag
     9181 aagaaggttt tcctgaaaag gtagctagtg tgatccaaaa aacttacccc aacccacaag
     9241 ttgtccaaat gatttctagt cagatcgatg cagatcgaat ggactatttg cttcgggatg
     9301 cttatttcac tggaacagaa tatggaacat ttgatcttac ccgaatatta cgtgtgattc
     9361 gtccttatga aggcgggatc gctttttcca tgagtggcat gcatgcagtg gaagattata
     9421 tcgttagccg atatcaaatg tatgtgcaag tgtacttcca tcctgtttcc cgtggtatgg
     9481 aagtaatttt ggagcatctg cttcatcgcg cacacgagtt gtatcaagaa gatgccagca
     9541 gttttgaact ccattctcaa ttacttctgc catttcttga cggaagcttt actttagaag
     9601 aatacctcaa acttgatgat ggggtcttgg gcacttactt tactcaatgg tctgaatctg
     9661 ctgatcctat tttaaatgat ttagctaaac gttttttgaa tcgtaaacca ctgaaatctg
     9721 ctactttttc tggcaaccgg gattcaaaat tagtccaaga actcactttg ttagtagaaa
     9781 aggtcggata taatcctgtt tattacactg ctgtgaattc tagctacgat ctaccttatg
     9841 atttttatcg cccagaacaa ggcagacatc gcacacaaat cgagattttg cgaaatgacg
     9901 gaactttgat agaattatca caggttagtc agcttgttgc tgcattagcg ggacaagaac
     9961 aaggagatga gcgatttttc tttccaaaag aaatggttga tccgtccttg cgtgatcatt
    10021 atgatttatt tgacgaaact tatcaagaat ttgccagcca tatccgtaat ggcgcattga
    10081 ttgaaataaa ttaaaatata ttggaggaaa ttatgtctat caaactagtc gctatcgaca
    10141 tcgatggtac tttattaaat gagaaaagag aagtaacaaa agaagtaaaa gaagcgattg
    10201 ccgctgctgt tgcaaaaggt gtttcgatcg ttttatgcac tggccgtccc ttaccaggtg
    10261 tccaggaaca gttaaacgag ttgaaccttt ttcaagataa tgattacgtt attacttata
    10321 acggggcact cgtacaacaa acgaaatcag gtaaaatcat cgcccgtcac ggactgacac
    10381 atgaagattt cctagagatc gaagtaatgg cacgccgtgt cggcagtcat ctccattcga
    10441 tcgatgacaa agcgatctac acgcctaatc gagatatcag cgcttatacg atccacgaag
    10501 caagtttagt caagatgccg ctaaaatatc gaacacctga agaaatgaca ccagacatga
    10561 acatcgtcaa aatgatgatg atcgacgaac cagaaatttt agatgcagca attgcgcgtt
    10621 taccgcaaac tttccgtgat aaatacacaa cggtcaaaag tgcgccctat tacttcgaag
    10681 tactaaataa agaagcaagt aaaggtgcgg cagttgcgaa tctcgcacag caccttggta
    10741 ttgatcaaga tgaaatcatg gctatcggag ataacgagaa cgacttatcc atgattgaat
    10801 atgctggtct aggagtagca atgggaaatg ccgtttctct tgtcaaagaa gcagcaaatg
    10861 tcatcacttc atcaaacgac gagcacggtg tagctgaagc aatcaaaaaa tatgtactgt
    10921 aataaataaa aaaactgaga gaaagctgat ctactcgctt tctttcagtt tttatttttt
    10981 tcgatccatc tctcgactgc ccatcggtgg ctaccacgtt tcaatttcac aagcgcttct
    11041 tgagggctca cccaccaaat tttattggtc ttttcagttg gttctcctat acgcttccaa
    11101 gaatcagcaa caaagaaata tcctggatta taataataag ttgcacgata attcgagtaa
    11161 aaatactcat cagcttctcc cagataagaa gaaatttctg ccacaattcc catttcttct
    11221 atcagctcac ggacaatcgc ttcttctttc gtctcttctc cttcaatttc cccgccagga
    11281 agaaaaaaag caccatttgg agcctcgatt actgcaatct gctgatctct ttctatgaca
    11341 atatatgctg cataacgggt ttgataatca gcaaattgct ttttttcacc aaaaattggt
    11401 ttatccatga atagtttcca ccttaacagt tttattttct atccatttta catgagaaat
    11461 ttctcatgaa caagcacaaa tcaactattt ccttacttct ttttgttatg attgagtaaa
    11521 aggaaaagga gagatgaatc tatgaaaatg gctcacacat gtgttcgagt gaaagatctt
    11581 gaagcatcaa tcgaatttta tcaaaaggca tttggttttg aagaaagccg ccgccgtgat
    11641 tttccagaaa acaaatttac acttgtttat ttgacacttc ctggggatga ctatgaatta
    11701 gagttgactt acaactatga tcatggtgcg tatgatctag gagacggcta tggtcatatc
    11761 gctatagcag ctgatgatct agaaaaatta cacgagcaac ataaagctgc tggtcttgag
    11821 attactgact taaaaggatt acctggcact gcgccttctt actactttgt cattgaccct
    11881 gatggttata aaatcgaagt catccgagga tagttagtat ggggcatttt actcttgatt
    11941 gaataaaaaa caaacaccaa taattaaaat gagacgcaaa cttttcatta aattgaaaaa
    12001 gtttgcgtct ttttttaaca accattgtag ctataaggtt gccactctcc accaatttat
    12061 ttttaattca tttgaaagaa atcaaataaa cgaggtacct ttagaaaaca aaactgcttt
    12121 tcctcctatt atcacacgat cttcttttaa ttcacattct agatccccgc cccttgatga
    12181 catttgataa gctgacaatt ttgttttatt cattctttct gcccaataag gaataagatt
    12241 agaatgtgct gatccacata ctggatcttc attgattgtt aattttggaa agaaggttct
    12301 agaaacaaag tctttttgca cactttctgc tgtcacgatg actcctattc ctaattcaaa
    12361 atttttgata gctgtaaaat ctggctgcaa tttagccacc gttttttcat cttctaatac
    12421 gaaaaataaa tcacgtgcta agtaggcttc tttaatctta gccccaatcg cttcttcata
    12481 ttcagacaat atcggcactt ttttcggcat aatactagga aaatccatgt aataataatc
    12541 tttaattctt gtcacaaata gattaccgct ttgagtagaa aatttaatcg tttcatcagg
    12601 tattttgtaa tagttaaata aaacaaatgc tgttgctaat gtcgcgtgac cacaaagatc
    12661 gatttctcga tctggcgtaa accatctaag ctcaaattct tggtttttct tcactgtaaa
    12721 tgctgtttca gataaattgt tttcaatggc aattttttgc atcgtacctt ctggcagcca
    12781 ttcttctagc acataaaccg ctgctggatt tcctttgaac acctcatctg caaatgcatc
    12841 aactacgtaa taatctaagt tcattttaaa gcccctttct ctaatttgtt ataaatatat
    12901 tctgcacaac aaagatcttc aatagctaga ccaactgcgt cgaaaatcgt cggcctcgat
    12961 tctcctttat aaaatgtttt tttctcagaa actaattcgc ctaatgtacc aacaatacaa
    13021 gattcctcta aatctccttc tgccatggct ataagcaggt ccccgctttc tgttaaggca
    13081 gctgagtaat catctacata gatttgagaa gtttccatca acgatgtact tatctctctt
    13141 gtatcaggag taaatgtgcc cacagcattg atatgtgcgt cttcccttac ccactctttt
    13201 tgaagaaatg cctctttact agaggtcaac gtacaaatga tttccgctgg acgtactgca
    13261 tcttctactg atattgcatt tataaacgtt attttaggat actgtttttt agctttagcg
    13321 ataaaagtat ttcgtctttc ttctaaaata tcgtaaacaa acactgtagt gatgggacga
    13381 atggtagaaa tcgcagtcaa atgcgacaat gcttgttgac cagctccaat taaagcaagt
    13441 gtttctgcat gcggatttgc taagtaatct gttgccagcg cagatacagc agctgttcga
    13501 atccaggtga ctgcatttgc gtttatcaat gctttgggta gtccgttttg agcatcaaac
    13561 aagaaaatct ctcctaaatg agaaggtaaa tgatactgat gattatccgg aaaagcggta
    13621 atgacttttg tgccaaaata tctgttctcc cccaaataag caggcatcat cgcaaacata
    13681 tttttgcctt ttccgtcagg taaaatttcc accaatcgag gattttggct gattttacca
    13741 ctttcataat caaaaaaaca ttttttcatt acttgaatcg cttcttcaaa actaaaagaa
    13801 gctactactt cctcaaaatt aataattttc atagacaatt ccctcctcac aaaagattaa
    13861 cttctaaaat caatacaatc aatggtaaaa ttgtatggta tacaatttta aggagtggag
    13921 aaaatgccta ttaattctta tgacaattac ccgttaactt ggcgaccaga caaacaaaaa
    13981 ttgaagcgtc cttattatcg atcgttgatt caacaattag aacaagatat tttaaagaat
    14041 aaattacaga aaaacactca gttaccctcc caaagagagc tagctgatta tttagattta
    14101 aattttacta ccgttggcaa ggcttataaa tatggaattg agaaaggttt gctgtatacg
    14161 aatattggaa gtggcacatt tatttctccc aatgtctttg agtccatcac catctctacc
    14221 aatgaagtaa ccgaacatat cattgatttt ggtctggtta gttcttttga agaatgtaat
    14281 aaattcgttg tcccgtttat tcaatcaact agtaataacc caaatcttgt tggtttgctt
    14341 aattaccaag atcccaaagg cacaaatttt caattgacta ctgcagcaaa ttggttaaaa
    14401 actcaaggag tcattccgtc cttagaatct ctatctattg tttccggtgt tcaaaatgga
    14461 ttggctatca cactttctgc acttttctct ccaggtgata gaatagcaac agaccgatat
    14521 acatacgcta atttcattga attggctcag ttatatcatt tagagatcgt tccaattgat
    14581 tttgacgagc aaggaatgcg acctgatata ttgattcagg aatgtagaaa aaagaagatt
    14641 catggtgtct ttcttatgcc ttcttgtaat aatcctatag ggtttcaaat ctctcacaaa
    14701 agacgggagc aattagccga agtgattgaa aaagaaggtc tatgggttat tgaagatgat
    14761 atccattcat ttttaacaac ttactatcaa catacgatca ttcctccttt ccaaaattta
    14821 ttacctaacc aaacaatcta cttagctgga atgacaaaat ttatttgttc tggattgcga
    14881 gttgcttatc ttgtagttcc agtacatgtc caaagcaaga tagaacaagc aatttttaat
    14941 ataaatgtga aaacttctgg attagatgtg gaagtcatca ctcaaatcct ccattcaaat
    15001 gaagcaaata ggatcttaga aactaaatta agtcttacaa aaagagccaa tcagcttttt
    15061 gattctcttt tcaaagtaga aaaaccaacg aatcgtgtcc cttactatcg aattcttcca
    15121 gttgatcctg agattgaccc taagataatt gagaatgact ttcttgctaa cggtatccga
    15181 ctttatcatt ccagacgctt tactacacgt gcgcaaccag atgcattcgt tcgaatttcc
    15241 ctatcctcta ataaccttga aattttagct tctggactaa gtatcgtaaa agaaaaaata
    15301 aaaaaatatg aattataacc tattgataaa taaaggaatc aaatcttatt gattcctttc
    15361 aataaaaaag aggcactacc cgaggttcaa caacgggtag tgcctcaaaa aataaacgaa
    15421 cattttgcac ttaaagtatc caagaagcaa gaaaaattat tttattttct tattttacta
    15481 aatcactagg tttctgtcag aagatgaaat tatccaatca tgacactttc ttctggatat
    15541 ttgtattttg attgtgttct ttgtgctttt gtcatcagag agaagaccac agtcaataca
    15601 ccaactcgtc caatgtacat aagcgctata atgatcaatt tccctattgc agtcaagtct
    15661 ggcgtcaatc ccatcgtaag acctactgta ccaaacgcag agaagacctc aaaagcaata
    15721 tattcgatgc cagaagtttt aggtatcgtt tcagtcaccg ataaaatcat gatggcgact
    15781 acacacaacg tcaacgtaac aaaaaataaa gtaagtgccc gcaacactgc gccttgtcgg
    15841 atggtacggc caaatgcttc cgctctcgta cgtcctttca acatcgaacg catctggatc
    15901 agcaacacac ctaacgtagt tgttttcaat ccgccggccg tcgacccaga tgttccgccg
    15961 atgtacatca aaaacatcgt taaaatcaga cctgcatggc tcatatggag gtaatcaact
    16021 gaataatatc cagccgttct aggtgtcaca ctcataaaaa aggtgtttgc caatcgttga
    16081 aacatattcc cttccactag cgttgcccca ttgcgctctg tgaaaaagaa cacaacgaat
    16141 ccgcctaata aaagggtacc tgtaacggtt aatgcgattt ttgaatgaat cgtcatcttc
    16201 tttttctgat gatattccag taaatcacgc catacgatga agcctaaccc accagaaatg
    16261 atcaaagcag ataccaccat gatcaaataa acattttgct ggtgattggc taaactgtct
    16321 cctaacaaat caaaccctgc attacaaaaa ctagagattg catggaacaa actgtaccag
    16381 atacctttca accagccaaa ttcaggcaca aatacaaaag ataaagcaac cgtaccaatc
    16441 aattggatca caaaggccaa ttttaggatg taaagcatga gcttcatcac acctgatact
    16501 tcttctagat tcagcgcttc ttttaatata atacgtgtgc tgagactgac tttcttcttt
    16561 atcaatgcaa agcatagaat cggaaccatc ataaaaccaa gaccgccgat ctcaatcaaa
    16621 agcatgatca aaaattgacc agcactgttc caatgctctg ctgtattcaa tgttgtcaat
    16681 cccgttacac agacagcaga agttgcagtg aaaagcgcat cgataaaatt tgtcccatgc
    16741 ccactattac taaaaaatgg tagggtaagt aggccgccgc caactaaaat caggagaaaa
    16801 aatcctaatg cgagaagctg aacagccggc aaacgtttct ttatttgttt gctttgttta
    16861 atcatcgttt tcctctttta aaaaaagata ccacttagtc agtggtaccc atatttttta
    16921 ctttgatcaa ccttgttact tctgcgcgct cgttttcttc cagcttcata cgaatataat
    16981 agatcgtttc ttctaattga gggatcgtca tatattctaa tgcattgact cgccgacgtg
    17041 tcttttcgat ttcactcgcc atcagttgac aagttttttc gatttctgtc agttccaaaa
    17101 gttttggaag cagggtagta aaccgatcga ttgaccgatc cagttcggca ctggaattca
    17161 aatacccgta ttccaaaggt gtgtctgtca gctgttcatc atattcaaaa ttcatcacag
    17221 gtacttttac actcatgata tttttgtcta cgatagacaa atcaacgttt tccgccggca
    17281 aaacaaataa ttcttcgatg aaaggttccg tgatcgaagc atttgccaat acaaaatcag
    17341 acattgcttc tgtcatagac tcttccattt cttgacgcag ctggttgttc tttcgaatca
    17401 acaggatgaa ttgacgcatc agttcatctt gcttatcttt taataattta tgccctcttg
    17461 tggcagttgt caactgtttt ttcaatcgag tcaattccat gcgggtcgga ttcacattca
    17521 atcgtgccat cagaactcac ttcccttctg gcagatattt gtccagcatg tcgtctttga
    17581 ttcgttttaa ttcggttctt ggcaacatcg atagcagttc ccaccctaga tcaagtgttt
    17641 cagtaatcga acggttcgtg tagaatcctt gattgacata ttctttttca aaccgctcag
    17701 caaacttcgc atagatcttg tctacatcag ataaagcaga ttcacctaaa actacggcta
    17761 attcttttgc ctgttttcct tgtgcatagg ctgcaaaaag ctgattcatc gtagctgcat
    17821 ggtcttctct tgtctttccg ggtccagttc ccttatcttt taaccgcgaa agagacggga
    17881 gtacatcaat tggtggctga atcccactct tgtaaagctc tctagtcaag atgatctgtc
    17941 cttctgtaat ataaccagtc aagtcaggga ttggatgagt tttatcgtct tctggcattg
    18001 ttagaatcgg aatttgagta accgatcctt ttagtccgcg gattcttcct gctcgttcga
    18061 ataaagtagc taagttcgtg taaagatacc caggatatcc tcttcttcct ggaacttctc
    18121 gccgtgctgc agaaatctct cgcaatgcct ctgcatagtt ggtcatgtct gtcatgataa
    18181 ccaaaacatg cattcccttt tcataagcaa gatattctgc agcagtcaaa gccatacgag
    18241 gagtagcgat acgttcgatt gcagggtcgt ttgctaaatt cataaacatg acagaacggt
    18301 cgattgcgcc cgtttgacga aaatcttcca tgaaaaattc agcttcttca aaagtgattc
    18361 caatagcagc aaagactacg gcgaaatcat ctgatgaatc gagtaccgtt gcttgtcgcg
    18421 cgatctgagc agctaattct ttatgaggaa gaccagagcc agaaaatact ggtaattttt
    18481 gcccacgaac taatgtattc aaatgatcga ttgcagagat acctgtttgg ataaactcat
    18541 ctggataatc ccgagctact ggattgatta cttctccgtt aatatccaaa tgcttttcag
    18601 gcaggatctc tggtccgtta tctttcggtc gtcccaaacc atcgaatact cgaccgatca
    18661 tgtcttccga tacacctagt tctaaaggat ggcctaaaaa tcgtacagaa gagtcacgta
    18721 aattgatacc acttgttcct tcgaatattt ggaccattgc tttatcttct tggacttcca
    18781 atacttgtcc acgacgaatc tcgccatttt gcatacggac ttcgattaat tcttcatatt
    18841 tgacaccagc aactttttca accgccatca atggaccgac gacttcaccg atcgtacgat
    18901 attctttaat cattgctcat gcctccttct gaaacgatcg tttgcatcgt ttgcttgatt
    18961 tcaccaataa tttgatctag tttttctaat tctgtttccg ggatatattt gcttcgtccg
    19021 atcctttcac gaatcgatac ggtaccgtcc atgatctcac ttaaataagc acctaacgac
    19081 aacgcacggc gtccttcttc accaaaggtc aagatcatct tcaacatttt gaattgtttc
    19141 tctcttgaag tgaatgtatc gacatcatca aaagcatttt gctgcaaata atcttcacga
    19201 atggattttg ccacttctaa tgtcagacgg tcattatctg aaagggaatc aatccctacc
    19261 aaacgcacga tttcattcag ctgctgttct tcctgcaaaa tacgcatgcc ctcggtgacc
    19321 atggatgccc agtcagcttg aaggatatga tccatatact tccctacttc tgttgaataa
    19381 agagaatagc tttgtatcca atcgatcgaa ggaaaatgtc ttttttgggc taaactggaa
    19441 tcaagacccc agaatacttt tacgacccgc aatgtatttt gtgtgactgg ttccgaaata
    19501 tcccctccag acggggatac agcactgatt gcggttatac ttccttcgcg accttctgat
    19561 cctaatgcaa tgactcttcc agatcgttca tagtattccg caagccgtga accaagataa
    19621 gctggataac cttcatcacc aggcatttct tccaaacgtc cgctcatctc acgaagtgct
    19681 tctgcccatc gtgaagtgga gtctgccatg atagcaacgt cataacccat gtcacggaaa
    19741 tattccgcaa ttgtgattcc cgtatagata gaagcttctc gagctgctac tggcatgttt
    19801 gatgtattag caatcaatac cgtacgttcc atcaacgatt ctcctgtatt agggtcaatc
    19861 aattcaggga attcatttaa aacatccgtc atctcatttc cacgctcgcc acatcccaca
    19921 tagacaacca tgtccacatc ggcccatttt gcaatttgat gctgaacgac tgttttccct
    19981 gcaccaaaag gaccaggtac agctgctgct cctccttttg tcactgggaa gaatgtatca
    20041 atcactcgct gtccagtgat catgggtgca tccgggttta atttttcttt gattggccga
    20101 ctacgtctta ctggccattt ttgaagcatc gtcaaatttt tgtcaccatt ttcggtttca
    20161 atgacacata ctgtttcatc aatagtgaat gaaccttttt cgattgtttt gatcgttcct
    20221 tttatcccat ttggcaccat gattttatgt tttataactt ttgtttcttc tacgatcccg
    20281 ataacatctc cagcactcac cgtttctccg gcttctactg ttggttcgaa ccaccatttt
    20341 ttctcatggt ccaatgcggg caattgaact ccgcgcccta agaaattact ttgtgtgatt
    20401 tccataaagg tatctaatgg ccgttggatt ccgtcaaaca tttgtgaaat gatccctggt
    20461 cctaattcaa cagataaagc ttcaccagta gagcgaaccg gttcgcctgg accaattcca
    20521 gaagtctctt catatacttg aatagacgcc acatcgccac gcatctcgat aatttctccg
    20581 ataactccta gatctcccac tagacacatg tcttggatac tggcatcaga catgttctca
    20641 gccattacaa gaggtccgga gacttttatg atttttccaa tttgcaaaat ttctatctcc
    20701 ttttcatact ataagatatt ttgacctact gctttttcta cgtttttttg aatttcttcc
    20761 agccctatcc ctaatgatcc ttgatgattg ggaatcaata cgatagcagg tatcatttgt
    20821 tccttgtaac gttcgatcgt ttctgggctc aatgctgcac attgctctgt gacataaatc
    20881 acgccatact cattctttgc catttcttca attgcctgac gaacggtttg cttggttgta
    20941 ccataacgaa catcaaagcc aaacaatttg aatggcaaaa cagaatcttt gtcgccaact
    21001 acaccgatct tatgcgtcat agacttgtct cacccgcttt ctgattgttt ctgattcgaa
    21061 atggcttctt ttgccgataa ccagtaaacg aaggtttttg ctttctacct cttttgcgtt
    21121 caaaaaggct agtagtggca gtggtccgaa agcctgggtt tgtgcttctt gatacaatga
    21181 acttagataa tcatctttca actgttcaag ctttacgaga tcaatatttc cctcacggat
    21241 gacagggcgc agaaaatcag cataatctgt ctctaataga aaattcgtgt aatcttccat
    21301 atctttatct acaaagttca aaaatacttc tttttcgata cttcccgagc tggaaagtac
    21361 cgtcgtcatg aacccatatg ttcttttctg caatattcct cgagccatcg ttgtgatatt
    21421 ggtcaagtca ataaaagcaa tgacttctgt cagcaattct tgatatccta gcttatcggc
    21481 aagccgtcgc tgctctgtca aaaactgtcg atcataaatc acgtcaattc cctgaagaat
    21541 ggtagactct tcaaaataat cacgtacttc tcgtatactg tccataatgc tttcaggaag
    21601 ttcatttgaa acttgcgtat gaatggcatc cttcaaggtt tctaatgaat aaaatccatc
    21661 gtcgatatac aaatggtcca gattttgatt tgtcatctca gcttttgtca aaaccttcag
    21721 attatggaaa gtataacgca tcgtatagat ccaaacgatt tcaggctccg gtgctgattc
    21781 ctttaaccag cgaaatagac ggctctgctc ctttaaaaga ttctgttcga agtcatattc
    21841 atatccgtca taaatatatg gactgtaaat cgtggatttc aatatttcac caagtgcttc
    21901 gacagaattt gtttgaatca tccgttcaaa tgtctcattg gacaaaagtt caagctctct
    21961 tccgcgaatc aagggattta gttcgtggta ttccatacac tgatccctcc ttattcgaat
    22021 aattgattgg caatctcaaa actcatcgtt ccttggatat cttgtacaag gctgctgaaa
    22081 agaaaattat actgaactcc ttgatcgttt atcagaaaac ccgcttgatc agcaacagtt
    22141 tcatcactta aaaccatcat aaagggaagt tcattgttcc attcatccaa caacgtctga
    22201 gaaagatagg ctgctgattt ttctccagca atgaatgcta cttttcctgt caaaggtaat
    22261 gaatacaatg catttttgat aaactggatt tgttcagatt catcccaatt ttccatttca
    22321 gttaccgcat ctgcaaacaa tcgtcttaaa aattcctgct tcgcattcaa attttcttgt
    22381 cgtacttcta cttgctggcg attccttagc tgacggtatt ttttttcgag taattcgatt
    22441 tgtttttctt tttgtttttg atgttctgtt tcaacttgcg tacgtttttg ttcaaagttt
    22501 tgatcgatct tcattcgctc ttcttgttct aacgccgctc tttcctgctc tgctgcttcg
    22561 ttcatctgag agatgatttt ttcaatggcg ttcatctgct tttcctcctt tatgcttagg
    22621 cattcaacac taataggaag gaaataacga aacctaaaat cgcgtatgtt tcaaccatcg
    22681 cagcaaaaat gattcctttt gtagcatgtt caggtttttt cgctaaaatt tgaatacctg
    22741 ccgctgcgac ttttccttgg gcaatacctg aaaacaagcc tgtaaaagca atcggcagag
    22801 aagcacctag aaagttcagt ccttgtacaa ctgacatgtc atttcctaag ttgataaaaa
    22861 tcaaaaaggc aataacgaat ccatacaaac cttgcgtgcc tggaagcaat tgcagaatca
    22921 gagcctgccc gaatttttcg ggttgacttg ttgtcaatgc tgctgctgcc tcacctgtca
    22981 ttccaacacc tttagctgaa ccgatccctg aaaaaatcgt cgctgttgcc atcgctaata
    23041 ccgcaaatac cattccacca ttttgactaa ttaagtaatc catcattata taaaaatcct
    23101 ccaattattt attgtttttt ttcttgtgat tgatgttgac gtatttttcc gccgttttca
    23161 gcggatcaaa ggcacgtccg cctcctgtat aaaacttccc aaaaaattcg acatactgca
    23221 atcgtgcgcc atggacatag gcacttagca gtgtcaaaaa catattcagt gcgtgtaaag
    23281 cgatgatcaa aaggatccct acactgaatc gagcagctgg cggcatgaaa gcaactagca
    23341 tattgaatgc cgctccaata cttccgcctg aaatacccaa tgccatcaaa cgtgtataac
    23401 tgactaaatc accgatatag ccggtcagtc cgtaaagatt atacgcacct ttggcagcac
    23461 ctttgacttt agacggcgtt tggataatcg gaacgatcaa gatacataag gcagcaatga
    23521 tcgatagccc agcgccaagg taaacaaatg ctgtattttt tatcaacatg gcaccaacca
    23581 acgcaataac gatcccaatt aggatccctt gccaggcaaa tccatcactg acagcactca
    23641 agtaatcttt tctcctgata ttttcctttg ctgccacaaa taatccaacc aaaatctgta
    23701 tcagaccgaa aatcacggat aaaatcaaaa tcgtattgac gtcatccgtc gtagatagga
    23761 ttgggaaagg caagcggatg ccaaacagtt cttttggtaa tgactgtccg aaaaatgaac
    23821 tgtagatcaa tccccagatg atcgatggta tcgagagaat ctcaaagaat tttacgaaac
    23881 gtttcatccc gcgtggtaga acaagtagtt tttgagctag tattgtccca atgagcatca
    23941 gcaaaccata accaacatct gcgaccatca tcccgaagaa gacaagataa aatggtgtca
    24001 tccaaggtgt tggatctact tcttcatatt ttggcagact gtacatttca gtcagcaatt
    24061 caaaaggttc gacaagcgga tggtttttca atttagtagg gacttcctgc tgaatctctt
    24121 ctgagtttgg tgtttcgaaa ctgacgtata tatcttcttc tgatacggtt tcgtttaaca
    24181 catgcagcaa attctgtttt tcttctgcat ctacccaacc ttgcaaaacg atcagataag
    24241 tcgcatggat aaaccgttct tttatttttt ctcgttctgc aacggcaagt gtgacttctt
    24301 ctacccattc aaattcttgg atatagccac tgcactgacc taacgctgtc gttatcttct
    24361 tttgctgctc gtgaacaatg gataattgct gcttggtctc ttgcagtttt tctttaggat
    24421 tttgttcata aggatacgtt tctttattga acccgtattt cccagcaatt tcttctataa
    24481 cagcaactga tttttttaaa gcaacatacg aaagataaac ttgttttggc gtataatcta
    24541 tttcttccat ataaacgtca tcaagcttac tcagttcttc cttgaaactt tcaaaatttg
    24601 ctgcactcac agtccccatc tcaaaaatgg tcgtcttact tgcatacata cttggagcaa
    24661 tatccagctg ttgccaatga agaagccatt cttcctcttc ttctaaacga tcacgttctt
    24721 cagttagttg ttcccacttt tcttttaaat caaggacttc tttcaattta tttatgaaat
    24781 tttcttcgga ataattggct tccaaatctt gtaaggaaag ttcttgacgt ttcaaatgga
    24841 gtttcttttg acggctatcg ccgtggtgtt gtatgaattg gatagcttct tggatgtctt
    24901 tcaaccgatt acctaaagcg cttaattttg cttctttatc aataatcgtg gtatctggaa
    24961 aatacttctc cacccactgg ttgtttgttc tattttgata cagatcccgg atttcaacgt
    25021 tttggattcc ttgtactgct tgtaaaatcc tctcctgatt tcttttatct gaaatgaggg
    25081 tcactttttc catcttagtg acggccataa gtttctttca ccctttcaat gatcgcaccg
    25141 atcgcttgac tctgatgctt ttgatattga tctttcaaag ttgcttgcac cttgtctgct
    25201 tctgctttca atttcatttt ttctttttct aattcttgta tttcaaattg ttctttttct
    25261 tccaccaact gttttagttg ttgtcgatag tttgtttgga tcgtttccaa ttcctgctct
    25321 ttttctgttg catagatctt cagctgctgc cgcagtgttt gagcacgctt ttcattttct
    25381 tcttctgctt ttcggatttg ttccaaagct tctaacactt gcttcacttc ctttcgattt
    25441 aaaaaagagg tacatgcttt cttttttcat tgaaagtatg tacctctaaa gataacttag
    25501 tataggcagg ccccttcaat aaaatgtcct tctcacccct tgaagaacta gatccttgaa
    25561 gcttacgccc aaccttttta tttctgctta ctttttatta taacaaatta agcaatattt
    25621 tcaactatcg tcatgaattt cacacgaaat ttctctttac agatagtaac acagcaagaa
    25681 cagataagta taggggcaag tcttacatag tttaattttt tttaaatcct tgttacaata
    25741 agtctacttt agggggaata caaatgaaac caatcatcgg cattgccgga aatcaactga
    25801 ttcgagcaac agatactttc caaggaaatc aagtgagcta tacaccgcaa ggattcgtgg
    25861 atgcagtttt agatgctcaa ggattgcctc ttattctgcc agtcatgtct ccagattcag
    25921 ctcctcaact gattggacaa atcgacaaac ttattcttgc aggtggtcaa gatgtcagtc
    25981 cacaacttta catggaagat cctcatccaa agctaacgga aacaaatatt caacgagacc
    26041 aatttgaaca ggcactgatc ttggaagcat tgaaacaaag aaaaccaatt tttgcagtct
    26101 gccggggact ccaactattg aatgtcgttt tggaaggtac actttatcaa gatctttcat
    26161 tatatccaaa gtggtcagta aaacatgaac aacagcccac tgccccgcaa tttacgactc
    26221 acgaagtcaa gattgtttca gacagtttgt taagcaacct tctccccgat ccatattttg
    26281 tgaattctta ccatcatcag gcagtcaaag atctttcacc tttattaaaa gccatcgctt
    26341 tttctaacga tggactcgtt gaagcggttc aaagcaaaga tgatatgcat aaaatcctcg
    26401 gtgttcaatg gcatccggaa cttacccacc gtgtacatcc atctgaacag cgcttattcg
    26461 atttctttgt gaatgaattt taatagactt tctgaggggg ttgtgaaaaa gctgtttagt
    26521 ttcgagtatt aagaaaaatt ttgtgaaaat ggcttttcac atttttgcat aaatttggct
    26581 taataccgag aaactggctt ttgaacaccg tttataccaa gaggtcgtga gcctgccgtt
    26641 caactctgag tcacaaggag caatttcaaa aaacagcttt tcatgtttgt tgaatagtgt
    26701 gacttgtgac cgaggagttg gcagaagaac accgtttatc cggaaaaaga gactgcgaca
    26761 cgacttttgt ctcagtctcc agtctatgga acaatgttca ctggaattct tctcaatcgt
    26821 cctttcgata gtccaacagc cttttgagct gggtaatctg ctgctgcagt tccatacctt
    26881 ttgttgtatc acgattgagt ttgcgtggta gttgctgttc gattactttt ttcacatttg
    26941 tttggtcgga tttttttgca aattgttgtt caataggcaa aaacggataa tgtgtcacga
    27001 tttggaaacc ataagaattg cataatagtg aataaccggc aattcctgtc gttttttgat
    27061 aagctttgga taaacctccg tcaatgacaa atacttttcc atttgctttg atcggtgact
    27121 cccctttttt tacttttact ggcgtgtgcc cattaatgat tgctgaccct tcttcttttg
    27181 tgtcgaattc tgctaaaatt ttttcactga accactcttc atctcgcaag tgataataag
    27241 gattgctttt ctcgacgtgc gtatgaggct ctgtgagaaa ataacgttca aaagtcgcca
    27301 ttttactctt gccgaataaa ggcgaaaatg gcccactcca cgcataccaa atcaaatcgg
    27361 ttgctaaatc ttcttttgca gtcagatctt ttgcgctttc agttatctgt ttttcaaaaa
    27421 agcccagcag ttcttttccg cgataagatt ggtctaattc aaatgctaag agctgtccct
    27481 tctcgtctac aggtatacag ccatgaaaca gcacatgctg attgttcgtg agatacattc
    27541 cccctttttc gatcaaaaac gtcatatgtt tttgcattct caaagagtgc tggaatgaat
    27601 acatcaaact atcaatcact ttttgttcct cttccaagaa tttttttgga tcatctggct
    27661 ggatcgtttg aaaacaagtt cctttcaact catagagctg accttctaaa tcaatcgttg
    27721 atgcttcata gtcgattttt tccagcaatt tacgatcgtc cattttaaat tcaggtcgac
    27781 gatccaataa ctgatgttct gatttgaatt gaaggataga aagtgcctga tggatttttt
    27841 ctaattcttc ttgctctctt tcggatagat ttttactgtt tttaggcgca aatactggat
    27901 tcttctgata agtttgatga gcaaaagaaa acaatggacg taaattcaac ccatattctt
    27961 tctctaattc aaataaataa ttatagcgtg ctgcaatccg taaaagtgtc agtaaacatg
    28021 cctcagagcc gcaatacgca ccaatccaca aaatatcatg atttccccat tggatatcta
    28081 cagaagaaaa atccatgatt cgatccatca ctttggctgc ttgagttcct cggtcaaaaa
    28141 tatctccaac gatatgcaaa tgatcgatga cagatttttg gatcgtctgt gcaagtttcg
    28201 ttaaaaattt ttcttcttcc ttcatcaaaa caagttgttc aatgattttt tttacatagt
    28261 ctttttttcc attgatacgg tcatcgatat acaagagttc ctcaatgata tagctatatt
    28321 ctttcggcaa tccttttcga acttttgacc gggtatattt gactgtcgta aaccgtaaaa
    28381 gatcgatcaa atgatggatc agctgcgatt tatcttgttc tttgtaccaa tcagtcgtta
    28441 atgcatattc tggataagcc gtaaaaatag tgaattggct gatatccgtt tccgtcattt
    28501 gttcagaaaa aaggtctttg atcttttctt taagatttcc tgaaccgata cgcagaatat
    28561 gatcaaaagc aggaaattcg ccatgtatat cactcaaaaa taactctgtt ccttttggaa
    28621 gctgcaaaat tgcttcgaga ttgatgattt ccgtgatgac atcctccgtt gaggggaatt
    28681 tttctgaaag taaatctaaa taacggtcca ttaaaacgcc tccaaaaatt ggtatactca
    28741 atattataaa gttgttatac actattttca atgaatatat atccaatata gtaaaataaa
    28801 taaaataggt ctatacaaaa agaagctggg aacaatagat gtccagcttc cttctgtatt
    28861 ttaggattgt tttatgcttt ttttcgttca ttcctcataa ttgaaatcga ttgtttcaat
    28921 gaactccata atccgccttc gctataaacg agtacattcg atttgtacat gtttgcagac
    28981 aagatcatca ataagaatgt tacggctaca agtaccaaca aggaaatcaa tgctccaccc
    29041 ataccgactg tctcatttgc taagcgaatc ggcataatat atgaagataa gaatggaata
    29101 tacgaagtga tccgaataac gatattgttt ggatctgaag ctcctagtat caatccaagc
    29161 atgtatccgc ctagagatag ataagtcact ggcaaaattg cttttgctgt atcttctgct
    29221 ttgttgacca aagagccaca taaagctgct aaaacagaaa aaatcaaaat gccaaagatc
    29281 atgaatagaa gagaatacca caagaatggt ccaatcaagt tatccaatga gataccgtct
    29341 aatatatttt tgaccatttc catatctttg aattgactgt atcctatacc aaaaataata
    29401 ccgtataatg ccatttgcgt taatgcaaca agtaaaacac ctgttaattt tccgtaaaaa
    29461 tgcttttgtg cagtcgtact ggacaggatc acttccatga tccgcgtgcc tttttcagaa
    29521 gcgatctctt gtgcaatgat ctgcgcatac gtcaaaataa taatgaacaa gacaatcgtt
    29581 gctacataac ttacgacgta ttggactgtc gagttatctt ctccgactgt catttttcct
    29641 tttgaatcaa aattgacttt ttgtcgagtc aaacttgctt gttggctcaa gctagctact
    29701 tcttctggtg aaatgcctaa ttgactcgct cgaagcatcg attgcaagcc agtcagttgt
    29761 tgttgtatca gtagttgtgt actttgtcct aatgaatttt cgctataaag catcccattt
    29821 atcttatcat tctcagttga gactactaaa tacgcatcga ttttttcttc cgaaagctct
    29881 ttttgtgctt tttcttcaga atcaactgct ttgaactcaa aatcatctgt tttgacttgg
    29941 ctcatttgct gactgatttg ctcattatca gccaatacgc cgattttatt gacatcacta
    30001 ttttgttgag caaagtttcc tgctacataa atgataccca gcatgataaa agggactaat
    30061 atcatgatca agaaagaaat cgacttaaca ttttttaaat agacatcttt ggtaataatc
    30121 caaaacttac gcatcgattt cacctgcttt caatttgaaa atctcttcca aagttggcgg
    30181 ttgctggtta aacataggaa tgtagccgaa ctgagtcgca cgagtaaaga tttctttccc
    30241 agcttcagga tcttctaagg taatctctaa cgtattgtct tcatgaagga tcactttttt
    30301 cacgccttcg atttcttcta cttcctgttg gcttaaacca gattccaaga ataacttcgt
    30361 acggccaaat gattcacgaa tctcatgtac ttttccattc aaaaccattt ttccgtttcg
    30421 caacatgatc aaatgatcgc agattttttc tacattatcc atattgtggc tagaaaaaat
    30481 gacacatgaa ccatgttttt tcaattccaa tattccatct tttagcaatt cagcatttac
    30541 cggatctaac ccgctgaatg gctcatccaa aatgatcaat ttaggttcat ggattaacgt
    30601 ggcgatcagc tggacttttt gctgatttcc tttagacaaa gattttactt tatccgtctt
    30661 tttccctttt acctgaaatt tttccatcca aaagtcgatt tttggttcaa tctcttttct
    30721 tgatttccct ctaagttctg caaaataaag cagttggtct tgaatcgtta ctttcggata
    30781 aagtccccgt tcctccggaa gataaccgat gacatcataa tctttctccc caagatgatg
    30841 gccattccat aacacttctc cttggtcttg tgtcaaaaaa tccaagatca aacgaaaagt
    30901 cgttgtcttc ccagccccgt tttggccaat tagccccatg atttttccat cggggatttg
    30961 gaaagagaca tgatcgacag ctgtgtagtt cccaaaagtt ttaacaagat ccttgacttc
    31021 taacatttct tcactccttc ttcaagaaat cacatatatc cttagacacc agacgtaagc
    31081 gatttattat ttaccaacaa aacgtaaacg ctctttttgt tgtacataaa atgataggac
    31141 actttacttt ctttgtaaag tgccctttcc tcattcctga tgtaatttat caaatacgtt
    31201 ttgtgcgaca tttttcggca gtccgatttc ttgtaattct tctgtcgtgg ctgctgtgat
    31261 atttttcaac gatttgaact ctttaagcag catttttttt cgttttggtc ctaacccttc
    31321 aatttcatct aatcgggaag caaaactgtt tttactgcgt agctgccgat ggaaagtgat
    31381 tgcgaatcga tggacttcat cttggattcg ctggagtaag aaaaactctg gtgaatttcg
    31441 ctcaagcggt actacagcca aatctgtacc gaatagcaac tcatttgttt tatgtttatc
    31501 gttttttacc atgccagcaa taggaatatc gattcctaat tgattttcta agacatcttt
    31561 tgccacatct acttgtcctt ttcctccatc gatcaagatc aaatcaggta aaggcaaccc
    31621 ttcgcgaatc actcgcgaat atctgcgata gatgacttct ctcattgatg cataatcatc
    31681 cggtccgttg actgttttta ttttatattt ccgatattct tttcttgccg gttttccatc
    31741 aatgaatacg accatcgcag ccacaggatt cgtccccatg atattagagt tatcaaaaga
    31801 ctcgatacgt attggggtcg ggatattcat cgcgtttcct aaacgttcaa ctgctccgat
    31861 cgtccgttcc tgttttctgg caatcagatc aaaccgttca ttcaaagcaa ctgaggcatt
    31921 cttactcgcc aattcaacta attttttctt ttctcctcgt tttggttgca gcactttcgt
    31981 agctaacaaa gcttctacac tgggcttgtc gatcgtatca ggaatcaaga cttctttagg
    32041 aatgaaatgc tcattttcct gataaaactg accgaggtaa gtcaagaaat cttcttctgc
    32101 ttcgttatag aaaggaaaca ttgaaacatc tcgttcaatt agctttcctt gtcttacgaa
    32161 aaacacttgg acacacatcc atcctttatc aatcgcataa ccaaatacat ctcgatccag
    32221 taaatcagta tttgtcattt tttggcgagt catgattgtc tcaatggcac ggatctggtc
    32281 tcggtattct gcagcttttt caaactccat gttttcagca gctttttcca ttttctgatg
    32341 gatctctgac tcgatttttt cgtgtcctcc gtttaaaaag tttttcactt catcaacgat
    32401 tttcgtatag actttcggat ctacgtcgaa ataataagga cacaggcatt gtcccaaatg
    32461 atagtaaaga caaggctcct tcgtctgact aggtttgcat tttctcagag gaaataatcg
    32521 atcgaggatc ttctttgtct cattcgccgc accgacatcg ggatatggtc caaaatacag
    32581 tgctttgtct tttagtacct tacgagtaat caccaaacgg ggatattttt cattcgttat
    32641 cttaaggaaa ggataggttt tatcatcctt taacataatg ttgtatttag gatcattttt
    32701 ctttatcaga ttgatctcta agagcaatgc ttcgatattc gattctgtaa cgatgtattc
    32761 aaagtcttcg atttcactaa caagccgttc cgttttcgta ttatggctac cagtaaaata
    32821 tgagcggaca cgatttttta atattttggc tttgccgaca taaatgatcg ttccattttt
    32881 atctttcatc aagtaacagc cgggctgatc aggaagtaaa gctaatttgt ttttgattcg
    32941 ttcgttcatc tggtactccc cttcatattc aattaaagtt attatagcat gtctgtcaaa
    33001 aaaacaatcc agcctgacaa cgtaattata ctcaaaaaat attaatttga ataaccttaa
    33061 ctcaaccgca aaaaaaccga ggacggaact ggattcagtc ccaccctcag cttatttttg
    33121 ctgtaagttt ttataaatat tgatcgatca tttgacgtag ttgttctttt gaatgaacac
    33181 caacagcttt ttctactact tgaccatctt ttttcaacat taatgttgga atgctcatga
    33241 taccgaattg ttgaggagtt tctggatttt catccacatc cattttagca attctgaatt
    33301 cttcttcatc atattcttgt tccaactgat ccaaaatcgg tgcttgcata cgacatggac
    33361 cacaccaagt tgcccaaaaa tcaatcaata caagtccttt atctgtttct tcattaaatg
    33421 tcttatctgt aattacttgt gacatatcaa agacctccat ttttaaaatc tatgactagt
    33481 atagcatttc tatacttgga tagcgaacca tctgcttacc tttagtaagc tttacttaaa
    33541 tttaacaatt gtcgcaccat ttccgccttg gtttgccggt gcaaactcaa agcttttgac
    33601 gctccgatgg tttttaagat aatcagtaat tcccttgcga agtgcgcctg ttccttttcc
    33661 gtggacaata gtcacttgag gataaccagc tagaatggca gaatccaaat attgatctac
    33721 ctcacttagc gcttcttcgt atcgttttcc tcgaaggtcc aattgattcg gcacgtgact
    33781 actagattca gatcgtacag catggataac tcgttgtgtc ggctcttttt gaggagcaac
    33841 aggcgtcagc tcgtcttcgg acacattcat ttttaatata ccgatttcta cttgccactg
    33901 attcccgttt ttacgtaaaa gcgtccctct ttggccgtaa gtcgtaacca acacttcgtc
    33961 tcccggtttc aacgtttttt gttctttcgc cttcttcaat actttatttt tcttcaaagt
    34021 ttcttcttga tgtagatttg ctaattgggt ctttgcatcg atcaactgat gctctttcac
    34081 atttccttgc ccaatctgct gttgcatttt acgaatatca gcaatgattt tttctgcttt
    34141 ttcttccgcc tctgacacga cttcatttgc ttttttcttt gctttttcca tttctttttc
    34201 acgttcttca aagaaatagc tgtaagcttc tttcaaatca tcgtgaagtt cttgtgcctc
    34261 agaaacaaaa tgacgcattt ccagatattc ggtctctgcc attttccggc gattctctaa
    34321 atctgtgatc atttcgttta aatcttggct ctcatcattc atcaactgct tcgcttcatc
    34381 aataacttct gtatcaagac ctaatcggct agagatttca aacgcattgc ttcgtccagg
    34441 aacgccaatc agcaaacgat acgttggact taatgtatcc acatcaaact ccatgctggc
    34501 attaatcgta tttgcccgat tatatccata aactttcaat tcaggataat gcgttgttgc
    34561 catcacataa gcagactttt taccaagatc atctaagatt gcgattgcta gagcagcacc
    34621 ttcttgcgga tctgttcctg caccgagctc gtcaaataat accagacttt tttcattgac
    34681 ttttgataaa atatctacag tgttcgtcat atgagaagaa aaggtcgata gactttgctc
    34741 aatcgactgt tcgtctccta tatccgcaaa tacttcctca aaaataccga tttgactttc
    34801 ttcacctgca ggaatcggta atcctgcttg ccccatcagt tgtaacagac ctaaagtctt
    34861 caacgtgatc gttttcccac ctgtgttagg tcctgtaatg acgatggctt gatagtcttt
    34921 tccgattgtg atgtcattag aaactacttt ttcctgatcg atcagtggat gtcttgcttg
    34981 tttcaagacg acatgattgt cttcactgat tcccggcacg atggctttta cttctttacc
    35041 aaatcgtgct tttgcattca tcagatccat tttaccaatc acataagtat tgtggatgat
    35101 ttcttgacga tggggaacaa gttcagcaga aagttcactt aaaatacgtt gaatctcatt
    35161 tcgttcagcg atttggtatt gacgcaaacg attattcaaa tccaccactt gttttggttc
    35221 aataaataaa gtctgacctg aagcactttg atcgtggact acaccgccaa atacaccgcg
    35281 gtattcttgt ttgactggga taacgtagcg gtcatttcgc atggtgatga tcgcatcact
    35341 taaatacttt gcatttttcc cacggacgat cccatctaac tgttcacgaa cagcttgttc
    35401 acttcgccga atgttctgtc ttatgctctt taactcagga gaagcgtcat cagtcacgcg
    35461 tccgtcttca tcgattgctt cctttaaacg gcggctcaaa acagggatcg tgactaactg
    35521 atctgcccac atatacaaac gtgcaaattc gatttcactg tcagaaagat catcaatgaa
    35581 acgattcaat tcgcttgtcg tagacaatac tctggaaact tgtgcaagct ccactccatt
    35641 caagtctgct ccgatctcaa tccgtttcat atggggacgg atattttcaa ttttaggtat
    35701 agggatcccg cctcgcagac gttgcacttt caatccatcc tcagtttctt gaagccagtt
    35761 tgtgatcgtt tgtttctcac tcactggtac caattctgcc agttcttctt ttccttgtgc
    35821 agtcactaca aattgtctga ccatctgttt gactttttca aattccaatg tttcaaggat
    35881 tcggttattc attttatcac cttatctttc actatgaaaa aagagctgtg ccggacatac
    35941 gctacgcagc cttcttaaat aaactactct gtacaaaaaa cgaaatattt tccagcacta
    36001 ttttccttaa ttcaaaatat tcgttaccca aagctcatac atcttgttgg taagaaatgg
    36061 cgttctctca acgataaaac gagcgaaact atgctcgccg aattgatttt ggaccacatc
    36121 taatggaaca aaggcaagta actgcaaaat caaaaatata gtaacgtaag ccatgattag
    36181 tgacaggatg cctcctgcaa tccagtctac ctgtctcaag atcggcacat acgtcaatcc
    36241 atggacaaaa acccccacaa atcgggtaag cagccagcca attagcaaaa taatcagaaa
    36301 agcagttcct gcataaaaag cttcatctag gtgaaaggca aatacctgat caaaaaatgc
    36361 taactttgat gttggggtga cagctgggta tggaatgtat aattccaaat gacttgctaa
    36421 tggtttgtaa aaatgctggg cagcaataaa tgacaataca tatccaatcg cgaatatgat
    36481 ttgcaaagca aacccgcgac gtgctccaga aaaaaacgca attagcaata aaaataaaat
    36541 cagcaaactc agcatgctac gttcctttct cttctaactt ttttaaattt ttcttatttt
    36601 gataaaagga tcttaattag aagatttttg tttaatttgt ccttttcgtt tctcgtttaa
    36661 aatctgttgt gcttccacat gatttttaat ggtatgatct gcttgaccat tttctttcaa
    36721 tacttcccga gcttcgttct cgattgcttc aatgcgtttc acacgatttt caagctctgt
    36781 aaatttgatc atttttttct ttaattctgc tgtttcttct tccagctcca atattctttc
    36841 ttgtttcttc aattgatccg aaagcgcatt gacagccatc aagattgctg cttgttcatt
    36901 atccatttgt ggtgataatt gtttcaattc tgctaactgt tcattgatca gcttcgttac
    36961 gatatccata tgatgcttgg tttcccgtcc gataatagta tatgtttggt tggcaatcac
    37021 cgctttgtag cgtgtttttt cgtgcgccat acgaatcatt tcctcctatt tgatgaaagt
    37081 tttatttagt gtaccatgaa gttggttctt tcaccgttct ccacaaatat ttttcacata
    37141 cttaactatt atatgataaa ctttaaaaaa atgctatgtc tcgcaatctc ttagaaaacg
    37201 aaaagtcgtg agttgcggta tagttttatg tataaaaacg aggagaattt tatgagcaac
    37261 gtcattatca aagcaacaaa cgaccaactt agtcaaatga aagcatacta tcgctcttat
    37321 ttgttaaata aacagattcc ttatactagc tttgctgcaa aaaaagacgg gacgaccatt
    37381 accgcttata catccggaaa agtgatgttt caaggtacga atgcagcaat agaagctgat
    37441 aaatgggggg aggcacagga taaacaagcc aagccagcaa aaacagcctc ttctttgcca
    37501 aaaaatatca gtcaattatc cgttttaggt agtgatgagg tcggaaatgg cagctatttc
    37561 ggtccgctaa cagtatgtgc tgcttatgtg aaacgtgatc agcttgcaga attacgtaaa
    37621 cttggtgtta gagattcaaa agagttaaaa gatagccaaa tcatccagct tgctgacatc
    37681 ttaaaaaaaa ccatccccta taaactgctt gtcttatctc cagaaaaata caatgagatc
    37741 caaccaaaat acaatgccgt tcggatgaaa gtcgctttgc acaatcaagc gatccatttg
    37801 ctgctaaaaa aaatcgagcc agaaaaacca gaagcgattt tgatcgatca gtttacgccg
    37861 gaagcaaatt ttaaaaaata tgcgcgttta gaaaaaaatc aattacaaga aaaactgtac
    37921 ttcgttacta aaggagaaca atatcattta gctgttgctg ctgcttctat catcagtcgt
    37981 gccagctttt tggaagagtt agacaaagca tcagctgaag ctggaatcac ccttccgtca
    38041 ggagctggaa cgaaatctga ccaagtagcg gcaaaactat tggagaaagg cggcatgcct
    38101 atgcttgaaa aatatgccaa acttcatttt gccaatacag aaaaagcttt gaagctacta
    38161 aaaaaataat ggaactgtga caaaagtcat gtcacagtcc cttgttccga ataaacggtg
    38221 ttcaatcatc tgatcctcgg tattagctca acaaacggca aaatatgagg aactattttt
    38281 ccgtttgttt ctctaatact cagaacaaaa cgttgatttc acaacctttt tcttaaacgg
    38341 tgttctcatg ccaactcctc ggtcacaagt cacaatagtc atcaaacatg agaagctgtt
    38401 ttttgaaatt gctccttgtg actcagagtt gaacggcagg ctcacgacct cttccttaaa
    38461 cggtgtttaa aagcttgcat ctgcgataat aagcccggcc aaacgaaaaa tatgaggagc
    38521 tatttttgtt tggctgggct tatttattac tgaaaataag tggtcaatgc ttgtgtcaaa
    38581 cttgcagcaa tctgctgctg ataactgtct gttactaatt cctttaaatc actatcatta
    38641 ttcatataac ccatctcgat taaaatagaa ggttgaagat tttctcgaag aacttgatag
    38701 ttcccagtgg agattcctct tgaatccaga ggcagatcag tgaaggagtc agcaattgtc
    38761 tgtgctaatg tttcctcttg tccatagtaa taatatgtcg tgattccagt tgcttcattc
    38821 gcatattccg tagagtctgc gtgtaaactg ataaagacat ctgccttttt ttcttcactg
    38881 tagtcgcata tatctccaag ctgtactaat tcatctgtag tatgggtcaa ataaaccgtt
    38941 gctccagcat cctctagcgc cttttttact tttatggctg tacttaatgt tatatctttt
    39001 tcttctgttt gttcatcatt gcttaatgcc ccagtatcat ttccgccatg cccaggatct
    39061 aatacaatca ctgcatcaga tagctcttcc ggttttgttt gtatattctt tgtgactttg
    39121 gttccatctt cgagggctaa atatccagta tagccatcat ttgtcttgat ctgataagca
    39181 ttttttgttt cagaaagaaa ctccgccagc tctccgccgt ttatagttgc aagtggggta
    39241 gttagcgagt catctgtata tagtgtacgg tcattttttc cgattttgat taatgtctct
    39301 tgttctgtat catcaaatga ttcctttgtc tttgtaaccg tactgttcgt actgctgacc
    39361 gtttgtccaa ctatcttttt ttgaccaaaa acatctattt gaaaacagat aataatgata
    39421 atgattgcaa atacaaaagc tccaattatt tttttcattc ttgactctca cctcgtagat
    39481 ccagactaag ggccaaaact taaaatcagc ttaagtaagc aaaaaagggg ctgtaaaaga
    39541 tataaccgta gaaaataaca ttactgctaa aatgattttt ttcatttttg gcatatcctg
    39601 aaatctcctt ttgactattc gtggtgattc atctacccaa taaatagttt ccttcattct
    39661 acaaacaatt aatttcttcg ttattaaatt aaaacacttc ctggaacacc gacataataa
    39721 ataaaaatat accctgataa tcatgagaga ataacaaatt tgtatttatg aaaaactacc
    39781 tatataaaga agcaagatat aacgatcttc gtcatatctt gcttcttcac ctaactattt
    39841 tctccctata taacagcttt acgcttctcc taatacacca ttcaaatata aatcgatcaa
    39901 ttgcgccaca tttagcggtt cttccacagg tcttttcgac cgagtgatgt aaggtgctaa
    39961 cactaagaaa aaatgccttg ctgcgatttc ttttgaaatc ccatttctta gcacagattg
    40021 gtttgctgaa aagatttctt caaaaggttc acgataattt ttcttccata gaaagaaaag
    40081 atgatctctt gtttcctttg aaagattgct ttcaagatcg tgaagcatca gaaacaaatc
    40141 gaccagatgt gtctcaatca aaaactgcgc catttttgtc aaggttactt gaaatcccgt
    40201 ttcgcttgtt tgaacgattt ctctcaaagc ttttccaaca tcttttaagt ggttttctaa
    40261 aactgccgta tatagttttt ctttatcacc gaaataatga tagagattgg gctgggtgat
    40321 cccaactttt tgtgcgattt ctcttgttga agtgccgaga tatcccttgg acataaacaa
    40381 gtcagtcgct tctttcaaaa ttttttgctg tgtatctttg ttgtatcgat ggaccatttt
    40441 tccacctgct ttttggttat tctttttctt tcaatacacc atcttccatc ataaatattc
    40501 tatcgcattt gtcaatcagc cgtgtatcgt gagtaaccat gatcgttgct ttatttttgt
    40561 cttttgattc ttttgccaag atatcgacta cttctcgtgc cttttctgaa tccaaactag
    40621 cagttggttc atctgctaga ataatggacg gatcattgta caatgcacga gcgattgcga
    40681 ccctttgccg ttctccacca gatagttctt ctggatactt gttgatcaac ttatccacgc
    40741 caagctgctc aaataattcg tttgcttctt tttgacgatg ttcatgttta atcttatcta
    40801 ctaataataa ctgctttttt actgtcagaa aaggaaccag attggaagct tgcaaaataa
    40861 agccaatttc tttaaaacgt agtttaacgc gttcgctttc tttttgatta ctgaattctt
    40921 gatcattgat aaatacagag ccactgctag gcgtctgcaa tccgcctgcc aaagtcaaaa
    40981 aagtactttt acctgaacca gaaggaccga tgattgcaac aaattcccct ttatcgatac
    41041 tcatggttgt ttctttcaat gctttaacta cttggtcgcc atcttggaaa cttttttcta
    41101 cttctttaaa ttctattgct ttcatgttct tcctccttct tatccaattg ccttcaacgg
    41161 atcgattttt acgattgtcc gaacagaaaa tagtgcgcct aacacagcta ctactaacat
    41221 caacaagcta atgacgccaa aaaacagcca attgctttgg aatggaaccg catcaggtag
    41281 aacaagggat gttcccactg ttcctaatag accaagaaga ataccaacaa atgataaaat
    41341 gaatgtttgt gccactacag aacgcgcaat aaatcctcct gtgatccctt gtgctttcat
    41401 tacaccaaag atattaatct tttgcatcgt cagtacataa atgaaaatcc cgatcacaat
    41461 agccgcaata acgatcaaaa atccgatcat aaatccaaaa gtcaatactt gcgcattata
    41521 tccaggtaat tcattgatga atttagaaat tttgatctgt tctaaatctt caggtaaatt
    41581 actgatttta cctctgacaa ctattgcgtt aatccgtgca ttttcacttg tatcttcttt
    41641 ttcgaagcgg atttcttggt aagcattgat ggttgtatag agaactggtg agacattgaa
    41701 tttggcatga tccgtaaagc cagttagttt aaacgtcttg tcactaccag ataacttaac
    41761 tgtatctcct atggctagac catattcttc tttcaaacta atatcgccta ctgcctcatc
    41821 atcgttatca aaagcttttc cttcaacaag attgggcatg atgaattgat ttttatcgat
    41881 accgaagaag ctaacattga tttttccttc ttctgtactg tctttactag tcacgactcc
    41941 aggtgtttgg gcaagatacg ccactttccc accttcgact tcttctgcta tcttcttagt
    42001 aatcatggac atccctagat ttgaatttgc atctttcgaa agcacaatcg aatctgcctc
    42061 ccacttgtcg actgctgttc gattatcttg agctaagcca taagctaacc cagtcaaaaa
    42121 aaataccagg taggcgatca aaaacatcac ccctgcaact aacgcataac gtaactttga
    42181 atgcataatt tcatttaatg ctaaaaacat ttaactcctc ctcctgaaat aatcatttta
    42241 tacaacaatt ttatcacttg ataaattttt ggcgaatatt atgctcaaaa aattcttccg
    42301 ctatttcatt atatgaattt tttattctcg attgattaat atttattata tccccacaca
    42361 ataaaagacc gtgatatttc tcacagtctt ttatctactc tattgctatt tgctctctta
    42421 gctagtactt taaaagctaa ctcattgagg tagtgaaaac gagaggtgta ttcagacaga
    42481 aatccaaaat cctcaactgc tagctgtgga atcgtagttt tgtgtttcac gatatccgca
    42541 agcaggttca aatctgataa taagaattct tggcttatca gctgtccgcc aattatttct
    42601 aatgtatccg gatggtagat cagctttgca tttactgttt tgttttcttc gtcgtattgt
    42661 ggaaatagat caaattctcc tgaacacgaa ccggtatttt gttccaagaa tggcgcttca
    42721 tcttctgtca gccctacagt tcctaaaaag tagtctataa tgactgctgc tgttatcttt
    42781 tgtctgaggt cgtaagcggt tggctgacca ctcaaagtta gcgctactgc tcttgccata
    42841 tgtacagctc gagtaactaa aggtaaaaag agatgccgtt tatttttgtc aaaactgact
    42901 gggacgatat ctccaatcgc atagatgtcc ggatcacttg tctgcagata ttcattgact
    42961 atcacagtct catcctcata caaagccact ttttctttta acaatgtcgt attaggacga
    43021 gtttgggcag aaaaaatgac catatctgcc tttattgttt gagacagaag ttgtacggat
    43081 actactttct cttctgattt tttccaatcg atcaagaatt catttggata aaaatttatt
    43141 tgcttagttt gcagctgatt ttgaaaactg ttgatcatat ccttatcgaa atatcttgat
    43201 aaaacattcg gcatataatc aactagatga ataatgtatc ctttatttct caaagcatct
    43261 gctaattcca atccaatgta accagcaccg aagatcacta cttcttttgc ttcttttaaa
    43321 gcggcaagta cttgcttaga ttgcactaga tttttatagg tgtagacatt ttcagcttca
    43381 atttgtttat acaaggtgac aggagaggaa cctgtcgcta cgattaattt atcataggtt
    43441 atggtctcgt tttttccttc tttggatgcg aggatgagct gatgtttgtc tggatcaagt
    43501 tcagttactc tccattcggt gatcagtttt gccccacttt cttgaagagt ccgcatgtta
    43561 tgggcaactt cagctaaact acgtatcttt ccttttaaat aaaggttgat tccatttgaa
    43621 acatagccta gtccatcttt atgatcttca tcgatcagta aaacttctgc tttcggttga
    43681 agttttctta gatttagggc agctgtgatg ccttcatggg aagcaccaat gatcacgatt
    43741 ttcatgtttt attcatcctt aaattcaatt tttctaaata acattctttt aaaaattggt
    43801 tcaaataggg gaaatcataa ttatattcgt tgctagtgaa tacaaaaact ttagcggact
    43861 tctgtgacgg gtactctttc aattggctag tcaaaatgat atcatactgc ttttctttct
    43921 cgtattcgac taaatcatat ttgatcgtat tcagcaattg ttcctggatg atttctttca
    43981 cgatatccgc cattagcctt tcataaggaa gatcataagc aatttgcaga cgtaattcta
    44041 actgcttgat cagcgtccgt aaaatagatg catagcgagc aaataattca aagcgggcat
    44101 tttcctcgtc tacttcagta aaatcaacaa tagcaaatgt ttgtctgacc aatctttcac
    44161 ataaaaccag gaattgttta tcttctactt cagttaaccg ctgttttaat ccttcttggc
    44221 caaaaatatt catccatcct ctaaaatagg caaaatgagc gtgattttgc atcactgtca
    44281 acgcaatctt ttgcgaaatc tcttctggaa aaacataaaa agagaaatat tcttctatcg
    44341 attgtttaaa cgttcgattc aattgagcta ctacgtcgtc tttgtatttt tcgcttatca
    44401 aaaactcctg gacatgcgag ttatgtgtat ctaatgtaaa gttactgatc atgaaaagat
    44461 aaaaaaccaa cgtttcattt tgattccaag gaaaggcata ttgaaagaag taacgggtca
    44521 aaaatgtatg gaggtccaat aaaaattgtg tcgtttgaaa caatccaaca ggaagattgc
    44581 tataatctgg tttggatttt ttcgtacagc gcattcgctt cttcataatg gaaagccata
    44641 tttcaaattt caatcgtcct tcttctgtaa tagcaaaact aaactctttt tgcagcagat
    44701 ctaaaaaagt gtttcctgat aaatcactgt attctttttt gttttcaaga tagggacgtc
    44761 caaaccaaaa taattggaaa taaaaataac gaatttgact ttctggaccg gtcagccgcc
    44821 cgctttttat tttcagatga aactcattta aagaatcatt taattccttc actctgcgag
    44881 acaaagtagc cgaactgata ttaagttcca tgacaagttt ctgccgatca aatgagccat
    44941 tttccaacag ataaagaagt atctgatatt ttgtagaata gcgcaagtaa caatgataat
    45001 aaacatcgac tgctgctata tttccatgtc tgattaataa aatattccct tcttggataa
    45061 caatttccaa ttgtttgata ttttccaagt tagcttgtaa ataaaacaga tcttctttca
    45121 aggtagcccg atcaactgat aagcgggtaa gtacgttatt aacggaaacc tctccttttt
    45181 cctctaataa aagttggaaa agtttgattt gccgttgttc atttttttca agcaaactga
    45241 aaagttccaa gtccactcct tcaagcattt ctgattttaa cagcagaagt ttccttctca
    45301 tccttgaaaa agaggatctg tgacagttat cacagatcca tacaaattct aatcacttag
    45361 ctggttggcc cagctgtcag ttcccatgtg accatgcctt gatacgttgc gccaggttga
    45421 gcattcgtac tgctatttac ctctaagagt aaaccatcgg tatcgggaaa gtcaacgaga
    45481 acctctcctt tagctgttcc atcactctca taaatcaacg tgttttgacc ttgctgaatc
    45541 aattgctttg atccagaatg attgaagtat aattttgttc cgcttaattc ttgaccttga
    45601 tcattcttaa ataaagaagt caatgtacct gtcagttgcc aaggagacgt gcttggaagc
    45661 cgagtatccc gcacatcaaa agaaaaatcg ccttttgatt cataataact tgtcttcaac
    45721 ggtttgggga gatcgttacc aaatgtaaaa tcatctggta cattaactaa tgttaattct
    45781 tgtttgggga caagaattgt tttagcagat gtttggacaa ctgggttagg aagcttagca
    45841 ccatctgcta atgtaatatt gctggaaagt acagctgcct ttgtcagtat gctgacggat
    45901 tgtaaagaag tatctacagt atttaattga tagttgtaag taagatgaat ggtatctcct
    45961 attttgctca atagatctgc tgggacagaa actccttgtt gaatgccggt atctgcgata
    46021 gttgtaccat ttaattttaa ggaaccattg atataagtag tattttcagg aagtgagatc
    46081 gtgatttttt tgtcggttat cgcatcccca gattttgtta acgtgaatgt cacattttct
    46141 tgaatcgtat ctcctgatag agcagacagg ggatctcctg tctcaggttg tctgctaagg
    46201 ttttttagct gactatctgc actcacagat ggaaaattag ccactgttat ttgaggaaga
    46261 gtggtgattc cagcttgttc agactgccat ttgtacgcaa aagacggata catgttagta
    46321 agtacatcgt ccaccaagcg ataatcaaaa taatattgcc ctgaagccaa tgcaggtagt
    46381 gtagaagtag ctgtatttac acccccgtta tttgattgga tcgtttggaa aagttgatct
    46441 gtccatcctg tcgctaattt ttgggttcct ttcgaataaa gcctgtaatg gatcgaaccc
    46501 ttttttagtt cagttgctga ccaattggcc gtgacttggt acgatgcaga agaaaggagc
    46561 tttccccact gactatcggc tgttaagttt gttggtaaaa ccgttccgtc tagcttgtac
    46621 tgctccaata cctttaaagc aacaggagaa agcaatccgc cttgcgcatc tttcccactg
    46681 tacacgatat tggctgtctg attcaatcca cttgtgcttg ctggagtatg agtcagtatt
    46741 gctccttgtg taacgttttt tcctgtaaaa tagttgacta cgcgatcaaa gcgactaaat
    46801 ggcaactgat caaaacgtcc ttcagtcagc cccatcgtca atgtccgact attggtagag
    46861 cctggaaggt tctggttatt tccatccacg tattctgtgg tatcagcaaa tgtattatat
    46921 tttcctgttg aatcacttgg tgagatgtaa ctattccacg tatttatctc tgcacttgca
    46981 gaaaatgcat atgctcctgg attcaagttt ccgccagtta atttaggata aaatctacct
    47041 gcagctggag ctaaaatcga attgaacata cgatcttgga taatattatt cgaatcataa
    47101 aaatcaacag aaaaaacggc ctttttagac atagaaggtt gtaatttacc cttggtcaaa
    47161 attttgtttg gactcgtatc atatttatct ccgttttctg tcatcttcgc gacattagct
    47221 gtaacatcaa attgaagcgg gatatcgtcc acgttgaatt cgcctcgcgt aataatagca
    47281 ataatatctg tcaatagagt atttgtatcc cacgacccca tcccaaaacg caaggtatgg
    47341 cttcctgctg ataataaact tccgctagta accttgatgg gggcaccggc aatatacaag
    47401 taggcgttgt tccagtcaat tgcttttaat acatcactgc ttgatacatg ttccggcaaa
    47461 ttgatgatcg ccacaaatgc aaatccttgt gcagtgatat tcaccaaatc aagtacattt
    47521 gttagtttca catggctttc ccaatgaaaa cggaccgtgc cacctttcca aggatcattt
    47581 gaaccatcgt tcaaataagt agcagtcaat ttaggcaatt ggttagcccc agttagttgg
    47641 acaagcttcc cgccattgac tgcactacgt ccaccatttg gaggtacgat attttcatac
    47701 gtgtatggat cataagaaat ggcaggaggc gttgctccaa cagcttctgc cgaaggtgaa
    47761 aagataaata tacttaaaac aaaaagcgaa gtaaaaaaga aggagcgaaa aaatgaaaat
    47821 ttattttgta gcaacatgtt catcctcctt ttcctttctt ctccataaca acaaccacgc
    47881 aagaagccat aattctatac caatgatcaa ataaagaaaa cggatctttt gttcattcgt
    47941 ctccgggaat acgccatgtt gattagattc tttataaatc ggtagctgtg tctgctgttt
    48001 tgatgcgcca ttgactgaac cacttggatg cggaattgga cttagatcat aaaatgtaat
    48061 cgtacctgaa cttttggtat ctgcttggac tggctcttgc cagctgaaga acagcaaact
    48121 tgcaataatc cctcccaata agatcgatgt tttcaacgaa atcacctcat catgttgttt
    48181 ctcctttttt ctttttcttg tggacccaca aaaacagcaa aatcaaccat aacactagaa
    48241 ctgcagcaac caatcctgca taaagccatg ggaaagattg ttcttgtact gcaaaaacgg
    48301 aattttcatt tactttttgt gctttttctt ttttgatcgt aaagttttgt gtaaatcgcc
    48361 attcattact gtcgcctgat tttgctatga tttctgcgcg gtaatcgccg gcttttgctt
    48421 tatcaccagc taaatcaaga ttcaagtgaa aattcgaatt tggtgcaact aaaaatgcat
    48481 tagacgtatt ttcaatataa agctgatttt cataaaagat cttgctcgta aatatcaaat
    48541 tgctgataat cctaggctga ggattttgga aacgaagctg aacagttggc tttgcgttga
    48601 cctcgtccaa atctcctgat aaaagtttca gatccggttc tatcgtctct tgactttctt
    48661 gtaaaacgac tgcgagatta taagcaatac gatttttgat ttgtgctcct tctgtagcat
    48721 tttctgtttc agcttcttct ttaggtgaaa catgaatacc cgctaataca cgtcccttaa
    48781 agctttttgc tgggattttc acttctatag gtacagttgt ttctgcatgt gctgatacat
    48841 tgatttcctt tttcgcaatg agcaaaacgt cacggacatc aaacggcaat gtctcatcta
    48901 acgttgggtt tttttgacta taatcgatcg ttccaccatc actcgttacc gccggattga
    48961 cagagacctc aaatgttcgt tcttcatctg aagaattgct gataattact tctagtgtct
    49021 gattttgttc cggtgccact aataaatcaa aataaccgat tgctttatct atttggttct
    49081 ctggaaatac cggtttgatt ccaaagtctc ctgctgaatc ctttgcttgg ctgattgacg
    49141 gaaaaatgaa aaatagactt aaaatagaaa aaaacgaaac aaacagtcgt ttactttgat
    49201 tcattgtcca ccacatcctt tcaacaacaa ctgcggtaaa aaatccttca cctatagtat
    49261 tttcaccgca gtttgttgaa tttatttcat actctatgct actgggccag ccacgaggtt
    49321 ccaagtcaaa tcagccgtga atgttccagc tgctgctttc ggcactttca gtgtgatatt
    49381 tgctggatca taaacagaaa gccatgttcc cagaccttga tcttttgctg ctgtaaatat
    49441 tggtgtcgct gcacttgctt tactcaaccc agttacagtt tgactagttg gtccagtgga
    49501 ttcgttatct gacgtacttt taacagtgga tgttccaaaa tctaattcag cgccttgcaa
    49561 aacttgtgaa ccgtttacaa aatctgaaac cgaaactgtc acgttccacc cttgtgcttg
    49621 gccatctgca cctacacctc gacgatctga tacttggaga taaggtgtat cgttttttga
    49681 agcgtctact tgatacgttt ttgttccgct ttcaatctcg tgtgtaccaa atggcaattc
    49741 gggaactaca tccagtgtta aaggacctgt ctggccagtt gctggatttt ccggatctgc
    49801 tggctctact ggttttgaag gatcagtagg gttaagaggc tccacggtat ttgttggttc
    49861 agtaaaggta atatctcctt ttgaagtcgc ttgaccctca gcaaaagctg gtgttgccaa
    49921 gccgcctaga agaagtgttg aaagtaataa tgtcccaatc atgttctttt tcataataaa
    49981 tcactccttt ttcttagtga cctaagtata gtagagacag tatgcaaaaa atcctcatga
    50041 attcatccgc ttacttgaaa ttatgaccct ttttgtataa aagagaaaaa aacaataaaa
    50101 tcgtttacat gaatattctt ccttttttat taagtttcgt ttgtcaaatt aagttagaca
    50161 aatcatcttt tgtgacgtaa ctcaaaaata cttccattgg tgtccgatag tgtaatgatt
    50221 ttctaggaat atgatttcgt ttcgatgcca ccgattggat aaaggtttca gatacaccat
    50281 taaaatccat agctttatac aaaccatctt tacgtaataa gccatttgaa tgttcattca
    50341 atcctcgttg agaaggtgtt cctggatccg caaaataaat tgaaatatcg ttggtattac
    50401 taatcttttt ccaatttgag aattcttttc cacaatcaaa tgtgatggat ttaaataagt
    50461 tttttgggac acaacgaaac cattgattca gcctattttc aatgtcttta gcctgtctgc
    50521 cttcgggttt caaggtaatg atgactttag atagccgttc aactaatgtg atgaccgcgc
    50581 ttttatgttt tccacccacg atagtgtcac cttccaggtg accaaattca tgatcaaact
    50641 gggtatagtc tgtctttctg tctcggattg accggcgaaa cccttgtttt cctctttttt
    50701 ctttatgacc gtttggtttt ctttttccct tcattggtaa atcgtgagtg tcaaaggttt
    50761 tatctttaaa cattcgatac agtgtgcgta ccgaacaagc aataggaaac tctgctcgac
    50821 caataaggac atcaggagcc cacccttgaa caaccttttt ttgaatgtat tcctgttgct
    50881 catcaggtaa aacaatcgga tgtcttccgc agttacgttt gtttttctta tattgtttat
    50941 agtagctcat tgcgcttcca ccagatttaa gaaaacgata gaccttataa atagtttgtc
    51001 gagaacgttg taacagattg gctacttttg aaacaggttg tgattgattg aaataagctt
    51061 ctatcattac gagttcagtt ggtgtaagat ggttataggt catttgtact cactccttat
    51121 aattctttcg tcggaactat tttgagtgta gcacaaatga ctttttttag ttgtctagct
    51181 taattttaca atcggcgttt taaaggcagt aacaggatta aacattgaaa cataatcgtt
    51241 actaaatgaa atcaagaaat atactgtatt actcaattca ttgaaaatat ttatcataat
    51301 ttttaataga aaacactatt aaagagaaaa aagagaggta aatcaaatga aaaaatcttt
    51361 aaaacttgca tcagcagtta tattaatgtc ccaactcctt atagcaacac ctttccatgt
    51421 gttggcaagc gaaaattcaa catacaaaat tgcaaaagaa agaaccgaga cgactgaaat
    51481 gagtaattcc gatgtaacag cagagagtag tgaacttttg acaacttcat caagtgaaag
    51541 tagtacgact tcaaatgccg agtcttcaac aacttcatca agtgaaagta gtacgacttc
    51601 aaatgccgag tcttcaacaa cttcatcaag tgaaagtagt acgacttcaa atgccgagtc
    51661 ttcaacaact tcgtcaagtg aaagtagtac gatttcaagt accgaatctt caacaacttc
    51721 aagtagtaca agtgaaataa cttcttcgaa agtgacaaaa ccaaaccata tagaatcttc
    51781 aaaaaaggaa gcgccaaaaa cggaaggaca aacaaaaact ttttcgacac cacagcaaat
    51841 agaatcaaca gaatctttcc aatcacaacc aatactatct ggtaatgaag aaattcattt
    51901 tgacaaaaat gaatctgtag aaaattttat tatgaaaatt ggtgagtctg cgagaaaaat
    51961 tggtcagaaa tatgatttat atgcttctgt tatgattgca caagcgatct tagaatctgc
    52021 cagtggtcaa agccagttag ctcaggcacc gaattacaat ttatttggga taaaaggaac
    52081 ttataatgga aactttgtca ttatggtaac aaacgaagat ttgggtaatg gaacgcttta
    52141 tacgacccaa tcaaaattta gagtgtatga aaactatgag gaaagctttg aagattatgc
    52201 caaattatta actaagggaa ttagtggaaa taaagatttt tatgcaggag cattaaaagc
    52261 taatagtaaa acatatcgag aagcaactaa attccttaca ggacggtatg caacagatac
    52321 acaatactat ttgaagctaa atgaattaat taaaacctac gatttaacca actatgataa
    52381 agaagttaaa ggtccacaaa tgagtaagac tggataccaa gtgcctattc ataattattc
    52441 aataaccagt ttgtttggaa atcgtggaaa tgaatttcac cgaggacttg atatggcagc
    52501 tacacagggt gagccgattc atgcagttaa agctggtaag gttgttaaag cagagtttca
    52561 ctactcttgg ggaaattacg tggtgataca acacgaagat ggatcaaccg cgttatatgc
    52621 tcaccagcaa caatacctag taaaaactgg agataatgtt ttgcaaggac aaataattgg
    52681 ctatgtagga agtactggga atagtactgg cccacacctt catttggaaa tttgcaaaga
    52741 tagtagtctt tctcagagca tgttagttga tccgaaaacc ttattattcg gagactaaaa
    52801 aaggtaatca atctgacaaa gtaacaagaa aatggttgga ttcttagacc tttgatcagg
    52861 atattgagac tggaattgat atctcgatta cgatgggttt gacaaataaa acaagtccat
    52921 tatcgaatat cgagtgtatt attgccgtct ttatgctcat attttgagca ggtttcacta
    52981 gatgaaaaca atttatctat cttgaggatt tcgtgtacgt atcgttcagt cttgtattgt
    53041 aaattagtca taaaatcaga ctaagaaaaa ttggaaatat ttttttctga tttattaaaa
    53101 gttaattgaa attatatggt acttctttct tgggagggta ggtgcagtga aggaaagata
    53161 tggtcacctt actgttgttg aggagattac tcataatgga tataaaaaat atcgttgcct
    53221 atgtgactgt gggaatgaaa cgattgtata tcgatcaaac ttactaacag gtagaaccaa
    53281 aagttgtgga tgtggaaatt ataaaaatag gcataaatat aaaaatttga caaataaaac
    53341 ttttggtaag ttagttgcaa tcaagccaac taaactgcga agtgaaggaa ctgttgtttg
    53401 ggaatgtcaa tgtctgtgtg ggagaaaaaa gttagtatcg gcaaagcgac ttctaaaagg
    53461 tgatgtaaaa agctgtggat gtttgaaatc aaaaaagcat cacattgaaa ataaaaaatt
    53521 cggattactt actgcaaagt atcctttgga caataactgt tcatggaaaa caaaatgggt
    53581 ttgtcattgt gagtgtggca gtatgtgtga gataagttat agtaacttat tttttggaca
    53641 tactcaaagt tgtggatgtt taaaaaggca agaaacaagg acatgtgttg aagggacagt
    53701 aatagagtgt ctaacgtcaa aattaccgaa aaataataca agtggtgtta agggagttta
    53761 tcgttctaaa gagaaataga ttgcgtatat aactttaaaa aaaaagaggt actatttagg
    53821 atcgtatgat tctattaaga gtgcagctca agctagaaaa gatgctgaaa ataaattgtt
    53881 tcaacctttg attgacaaat atcgaggtaa tcattaatta ttgtggataa taattaacag
    53941 tgatatataa acatctataa tatattattc atactattga tatttagtat aatcctatgg
    54001 aattgaatat catagcaatg tatttgaaaa gcaaattgga aaacaaatag gtgcaacacc
    54061 ataaatatga aaaaattggg aaaaagtaga atggaaattt gcttacgtct cacgagatag
    54121 ctcacactat taatctgaag aacaatgaag tcgactttta gaaattgcta gaagaaagta
    54181 aagcgaatta tcactagtta ctatagagta agttcaaata aagcatctcc aacttacatg
    54241 gttacatgag gatatcaaat cgaaattact caagcttcct atggctagag tcgcgagaag
    54301 ttgttgggca gtagacatag gttatattgt tcttagctac acgatcatta gaaaggaaat
    54361 cctattatgt atagttatca agaagatgca aaagtccaaa aaaatcgctg gtggattttg
    54421 attgctatat ctatgtttac cttcatgtcc acattggatg gcagtattgt caatattgca
    54481 ctacctacca tctcagatga tatgggcata ccaatgaatc aagcggaatg ggtagtgtcc
    54541 gtttatctga tgattgtctg ttcttgtttg ttgctgtttg gtaaaattgg cgatagccgt
    54601 gggaaaatca aagtatttcg gattggtacg ctgatctttg tcttgggatc gctgttgtgt
    54661 gggttcaatc attcgctagg tattttattg ttcgcacggg tggtacaagc cattggtgct
    54721 agtatgacta tggctacaaa tgccggaatc atcacggaag tctttcccat acatgaacgt
    54781 ggtcgggcat tgggagcaat aggtgcgttt gtttcattag gctcgattgc tggaccaggt
    54841 cttggcggct tgattttgtc ccaattttct tgggcgtaca ttttttggat caatgtaccg
    54901 gtgggaatcg taacgatgtt gattgctgaa aaattcttac caaaagatat caccatgagc
    54961 ggccatacaa tcgatggagg gggctttgct gccattgcgg ctacgattat cagctttttt
    55021 ggagcaatct ttttggggca agaaagtggc tttttccatc cagtccccct tgtactgttc
    55081 gtaatttcag tgattgcctt tgtcagcttt ttaaaagttg aaaagaaagc agcctctcca
    55141 ttgatttctt tcggtatttt taagaacaag atttttacca tgagcttgat tacagccttg
    55201 ttgatttttt cttccaactt ctttgtcaat gtagtgattc ctttttatct gcaaaatgct
    55261 cgcgggttga gtcctagcta tgctgggctg ttgatgatgg tctttccttt gatgatggtc
    55321 gtcggggcgc ctttaagcgg ctacctgacc gaccgggttg gcccggagat tttggtatta
    55381 gtaggcttgg gcttattgtc ggtaacccag gtcatgtata tgtttttgtc agtggactct
    55441 cctttgtggc tatacattgt cgctactgcc atcatgggtg ccggcaatgc cttgttccag
    55501 tcccccaata atacgatggt tatgagctcg gtcactatcg aaaacttggg actggcaggc
    55561 tcgatgaatt cctttgcccg aaatttgggg atggtcagcg ggatttcgct ttctacgacg
    55621 gtcttatatc aagcgatgtc tgctaagatg ggccagcaag taactaacta tatcgcaaat
    55681 cggccggatg tctttttata tggaatgaag attacctttt taggcagctt tgttttgtgt
    55741 tttgttgcct ttttcctgac tatctggcgt atcaaaaaac aaggaggtat tcagaaaaat
    55801 gtctgaaaaa accactgttg aagaattttt ggaggcctgc gtatgggtga gtgggatttc
    55861 catggatgat ggaagatgaa gtgaacttct aaaagccgaa aaactcaagt attatcttca
    55921 aaatactagt gctcaatgag atgcttgatt tgatcacaag aagactgcga aagccgctgg
    55981 caaccgcaag ctaggtatcc caccgatgga ctacgtttta gaggctactg gttatgaata
    56041 cggagccaac gctctcatct gcataagaat acatcaccca gattatgtaa agattttcga
    56101 tacttctgtc aaaaaatcat taagatagat aaatgatttc cgtctagtca aattggttca
    56161 gaatgtagac acacagatgg taagaagtca ctcacaattc gagaatggac ttgtcctatt
    56221 tgccgtatac atcataatcg aaacgtcaac gatagtatca atattttgac caagggctat
    56281 gaatacaagc attgccttag aaaacatcaa gaaccttagg aacaacgggg atagcttggt
    56341 taataagaga taccgctgtt taggcttgca agtatactct tttatcaaga attccgtggc
    56401 tttagtcatg agaggcttaa tactatgaaa attttcttta gcatttttga tttaggttcc
    56461 ttttctaatt ataataagat gaccactaca gtaacctact gaaatatata aacagccact
    56521 gtagtaccat tttcaaagta gtctatgaga ggagaatctt cattcttatg aacgctggaa
    56581 ttgcggatat gaatttaata aaaaaaaccc ttaatgattt tacttccaat tcaatatcaa
    56641 aaggtactgg tatcaattta agcacaataa aaaagctgaa aagtggtgaa agatcagtgg
    56701 aaaagctgaa tcttttagat gctataaaaa taacggaatt tgctatgaaa aatggaaaag
    56761 ctgagattga aatatggaga taattatcgt ctagtgaatt acttgccact tagctgaatt
    56821 taacgatcct tggtgcttga agggggagat tcttgggaat agagcatact tgcaagccta
    56881 tgtctttact aacaacgata tctcttatta accaagctat ccccgtagtt ctaaggcttt
    56941 tacttttatc taagcaataa tcgtattcaa aggaaatttt tgacccttag aaagtctcat
    57001 tttatcagac gtgagcttag gcattgctga attttgttaa ttcggtttta ggttgtgctt
    57061 tgagcataat atgaaagtat tctttatcac gatttcattc atctaaggta atgtgatgtg
    57121 attctgaaat tctttcaaag gcagtttttg cataagctga cattacatca tcaatgacat
    57181 gtttacaatg tttcactact aaaataaggg ggtaaccaag aagaaaaact gaatgattat
    57241 ttgtatctaa ttccatggta tatcaaccat ttcctatata gactgattat gacatggatt
    57301 aaaataaaaa ggcactatgt cttaattgtc ggttttcgag ttaccgaatg gcattcatca
    57361 cccacctata gaggatgggc gacttctgcc tgaaataagt tacactactt ataacttcta
    57421 ataaaattag attgagtcat tattatttaa catcaacgat tttccagttt ccattttttt
    57481 gtaatactaa tttgtactga aagtagctag tggtttttga tacattatca aggtatttta
    57541 tctttgtact cactagaaaa ttttttccgt tttgtagata aactggattc tctaactccg
    57601 aaaactttaa gtcaatattg attggtcgca tcgcaccatt ttcaacataa tactccaatt
    57661 ctttttcaga agctgatgga taaagtttaa aaaatgtttc taaaaaatcg gtgatttctt
    57721 ccgttgtttt ggcatcaatt tctgaatcgt tttctacttg ttttgcttca tattttgcct
    57781 tgtctggctt tccaatcatt gttggcaaac tagttactaa atgattcctg ttttcatctt
    57841 caaagattgt cactcgatat gcagaagaaa ttgattgcgt cttttttccc tctgtaatat
    57901 gttgctccac taaaaatgaa acgacaaatt cttccgagtg tttttcaaca tctagaattt
    57961 ttacagattg aacttcggaa ctcgtgggaa tatctgctcg aaccatatct tggattaaag
    58021 cgagaccgtt ttctgtaagg tactgttcga gattactcat tctcttttca attgcttctt
    58081 tgttattttt ccatgaaaaa tattctttcg caaaatcctc tgtaaaactt tcaacgccag
    58141 aagtattcac taccttctct tttataacca ctttttcatg agttgtatgt tgatcaatgg
    58201 cggtgaaatt tttataaacg ccaaaagcaa gactacaaga caataaaagc caaagggcaa
    58261 gcaccatttt tcgatgctta cccacggata caactcgttc ttttttcttt tttgggctct
    58321 tttccttttg attccgttca atttttatct tcattctttt tcctcttttc tcttatttgt
    58381 atcggccagc tccgataaga tgctgccgcc aatagttttc cgttagattc gtccacccca
    58441 ctggatctcc tgcatgaaac atgcgattct cccctgcgta aatgcccaca tgggtcacat
    58501 aagtaccggc atcataggtt gaatgaaaga aaactaaatc acctggtttg gcatctttta
    58561 gatcaatatg agtcatggca tcatattgat cctgagccac ccgaggaagt ttcaggtcaa
    58621 ctttggcata acaccattgg gtcaagccac tacaatcaaa ccccgtttct ggacttgagc
    58681 caccaaacac ataggccgtt ccttcatact taaatgcctc atcaaaaatt tcttggacta
    58741 ttttgtcatc aaactgagtt gtagttaggt attgcttcac taataaacag taaaacatat
    58801 tgccataatt ataccgccac ccaccatttt ccttaatcgc gattgggttg gggtaatcga
    58861 ctttttgacc acctgatttt tcctttgaaa aagattcagc taaagcaaaa ctatgcttct
    58921 tcccgttttt cccgacatga tctagatacc cgccaccata gttataggcc tgaataatac
    58981 tatcttgatc acaccctagt tctttggaac gcttaaccaa agacgcaaaa tagagacagc
    59041 cttgtttgat ggatgcctcg gtacttaatg aattaggtgg taatccggct gactcagaac
    59101 tttgcataac gtcttccatt gtgccaccag attccacttg aataatcgcc aaaagaattg
    59161 gaatatactc tgttatatcc gattctttgg catacttttc caccattgca cggtgtttca
    59221 atacttcttc tgagacacta gttccgccag gagaagtgcc agaactacca tttccctcac
    59281 tgtcttcacc aaacaacaaa cccacacaca tcaaaatacc cataaaagag atggtcagaa
    59341 aagttaatga aataacaccc actttcttca agttcatttt ttctcacctg accttgattt
    59401 tggacgatgg ttcattcttt tttgtacttt ttgattggtc tgctttggca gagtggacct
    59461 ctgtttcact tgttggaaag aatttttctt acttgctgat tggcttttcc cttttgaaat
    59521 gatttcttga ctagttagtt ttcgtttgat ttcgggattc agatttgctc tcacaatccg
    59581 ctttggtgct tcttttttca gcatcatatc tttctgaggt tgagctgctt tatctattgg
    59641 aatattcgcc cttctttttc cctgacgatt tgttgcttca ctcagtatct tgcgtttttc
    59701 gtccattttc gcatttcttc gttgtcgttg tttttctcgc aaatccactc gatccgcttt
    59761 ttcttggaac agtccgcgct tgaattcttc tggtgctttt ttcgactttt cgattccttt
    59821 atggagattg tatttcaaat tcgttggcgt attccttacc tgatccttta cttgtttagt
    59881 cttatctttc accaaagctt gggtatccaa aactttgccg atttttctcc cagccaaatt
    59941 cattcgggat tgtttcttat tatttgaaac tggattctcc ttagttaagt cagatagtat
    60001 ttcgtgatcg ttcggtaatc gtgaattctt tcgtagagaa gaaccggatg gcttaaattt
    60061 ggattttgat tgttcctttg ccaacttatt tccgactgct gcaccaccac caaaagccaa
    60121 agctcgtccc acatttcgat tgagttgccg caattttcga ttcattaaca tttgcggttt
    60181 tcgcatgacg cgtcttccca ttgactggct atcattactc tggaggttga acatactcat
    60241 aatgtcgccc aatttgaaat agataccggc aaaggtcaca atttgcagaa aagcaaccat
    60301 gaaaaatgga taggttgccg aaatattgaa aaacatcgtg gagatgctaa atgcagtcgt
    60361 aatcaccaaa gtaacacccg ctcgcatcat aatcgtattg aataaacgaa taatcgcctt
    60421 ctttcctaaa ctttcataag tcggcaacat ggacaataag aaactaattg gcagaaacat
    60481 cgcaaagata atgaagagaa tttgcgagaa caacataatc cctgttagaa gaaaaacaaa
    60541 gaaggaaatg ccaatattga acaataccaa aaagaccacg gtccctaaac ggttcatggt
    60601 cttggtaata ctgagattcg cattgtcatt gtcttcaatc tctgccttga ccgcttcttc
    60661 ccgatctttg cctttgtttt catctggact cacagataga atcttgttga cccggtcttc
    60721 accaatttct tctatgttgg aatcatcaaa ttgcagtagt aaccaaggtt gttggacttg
    60781 aatcgaaaac aaactatccc gaatcaaatc cacactatct tttccttggc tttctgaatt
    60841 gggtacaacg atctcggttc caagggtaag ggccgcttca ctaacatctg agctaaagtc
    60901 attgattttt gtgatatagg taggcgcata agcaatgaac gaacccgata gtagaaaaat
    60961 catcacaaaa ttaatcacgg ctcgaactgc tttcgtggtt tctcgtttta ataatccggt
    61021 atacgccaca tacaccccca gagctaaaat catcaataat agaaacccaa aataaaatcc
    61081 ggaggtctgg agtccatttt cagtaatacc agccaatgtt tggatattct ttccaatact
    61141 ttcagccgta tccgaaatga aatctaattt gtaagcctct tgaaccacat aacccgtggc
    61201 gttcgacaaa tacaaactca ccgtccaaat gaaattagta atggcataaa gaccatacat
    61261 cacgcttttc cctaagccat caccccaatt ccaaggcaac caatcccaag agctatccac
    61321 aaagaaatct agttgatagt tgttcagtga atatttggaa tatagatttc ctgcttcaac
    61381 tgtatcatca actagccctg tcgcctcggc taccgtacca actaaactca atagaactat
    61441 cagagtgagt atgctaataa aaacgattcc cacaatccga aaaagtttct ttttcatccc
    61501 ctcactccat tctctcttga acaggtggac gggtatcaaa ggcatggaag agttcttcaa
    61561 agactggatg tacctgaacc acgcctaccc gaccatacaa atcctgcatc aagcattggc
    61621 cattttccaa atctcgtagg cgtttttgat tgctttcatc ttctcgatcc aagccaaaga
    61681 attccaaggt tttcttgatt tcatgaatat cggtactgcg aaacgcaaac ttcaacccga
    61741 tattattctt caagcgttca tccagtaaat catcactatt ttgcgtgaca aagtaaaccc
    61801 cggcattcat agcccgccca gcacgaacga gcttattcga taatgttttc ccttgcgcta
    61861 cttgaagaaa actccacgcc tcatctaagt ctacaatttt gaagatttct cgattactat
    61921 ggataaagtc caaggcaaaa gtggaaatga cgattagcat ggaaacacta agcaattcca
    61981 tggtggtata ttcttcaaaa gtggtttctg catctggtaa aaccaaatcc gctacttgaa
    62041 tgatatttaa ctgcctatct aaactaatcg actgggtaat cgaaccatct gagaaaagca
    62101 aatgggcaaa atcataatcg gtaaaggact cgatgtgatc cgcgatattt tccgcaactg
    62161 gtgagccttc ttttcgaagc tcattaatta cttgtaataa tccccgttga ttgctttggg
    62221 taaccgtccg aatggcctta cgcaacactg gaaacttctc cccatctcga ctagaaattc
    62281 ctgttaaaaa ggtcagaata tcaattgcta gactttcaga atccttcttt ttctccataa
    62341 tcacataagg atcaagtaat ccttgatttt ttggcttact agttaagttg agaatattga
    62401 tttcatccgc aatttctggc aaggtttctt tccagccacc ccgttcccct tttggatcga
    62461 caatcacagc ttgtccacca aaaaggacgg cgtaataaac taagagattg ttactaaagg
    62521 actttccgcc accgagtgaa ccgaggaatg ccgctgctag cgcattggta actgaatcct
    62581 tgaccccttg tgaggccaat gccggtttca ggtaaacatt gcgccccgta tctacgttat
    62641 aaccaaagta aatgccttcc aactccccta acatctgagt ggcgccaaaa ccaagtccgg
    62701 cgagaaaatc agacgtaaca tactggatat aatcattcat gtatcgctta ctagaaggta
    62761 agaattcttc atgtagcccc atcatatcgc caaaaggccg gactagtttt acattggtat
    62821 catcataaaa atctagcact tcatcacacc ggcgctttag ttcatctact gattcggcac
    62881 ttactcgaac cacatagctc agtttgtaca tggattcttt ggactgatcc aaggtagttt
    62941 ccaattcatc tacggaatcc aatgcatcta agacattgga atttgtttca ttgtcagatt
    63001 gataggcatg gttgtccaag tcttttaatt cctttttctt attgcgaacg gtagcaagtg
    63061 cctttttgtt cgtcacaatc tccacattca tactagtgtc aattgggaat gtaaattgtt
    63121 gttgctggta atagaaaagt tctgaggagg gaaactccat ttcccccaca attgtattaa
    63181 ttgttaagta cgctacatat gattcatgat tctcatgttc catgcataag taccggggct
    63241 tttcttcaat caaacaacga ctgggacgta ataagtcgta acgtttcacc aaagtttccg
    63301 acttcagctt tttctttggt agctgaaatt catattcttc aaatggtgta cccttttcgc
    63361 catagatatg ttcaatcaaa tacatcagat cactcggtgt gactcgtctg actttaaatc
    63421 ttcgagccaa tttactttcc attagttttt caagcttggt atatcggcga atttcttcat
    63481 tggataagga aacaaaatcg cccatcaaat gatgattcac tccatagaca aattcttgaa
    63541 agccggagaa gaaagatttc ttgagattct ttagattgac ctcttcatct gtcgcaatca
    63601 gtttgaagcc aataaaaaag cgataatcaa tctgactatc gccaatcatg gatactaatg
    63661 cctctgtctg taaatcaatt cgttgtttcg ctacttctgt taatcgcccg gtaatttcct
    63721 tttttgatcg ttcttgcgtg gcacgaatac tactttctgt ggcaatttgt aaggcatgaa
    63781 tcttgccttc acgattttgc gcaatcaatt gacgaaagtt atcgtggact tgatatttct
    63841 gttcaggtga caaaaaggag taattgtaag gtattaactc atagtaggca aaacattctc
    63901 cctcttgatt aaagacaaga ttattttcaa tatacttaat cgggtacata gttcacactc
    63961 cttaccagag taatgggttc gtcccatcgt ttcttctgtt ctttgacttt ctttcctgcg
    64021 aaagtgacct taaatcgtaa gtgatagcta ataaaagatc gtaaaaatcc caatggtttc
    64081 ttaccgtcaa acgttttttg cgacatgaac caagtaactg ccaccggtat acctaagtat
    64141 tttaaaaagg ccccctctat catagatagt ggaggcacat gggcaaatac gatgacaaac
    64201 agcaaggata aaacaaacca agccatctgg ttaaaagtca caggaaaagg cagttgaaag
    64261 tcattaattg cataaatgac cttttcaact gcccaaatac tggtgtagct ttttattttc
    64321 ttcatcaaat cattccttta aaaagaagga tagtttagaa atcaactacc cttcaataat
    64381 tttgttaata tggatactca aaaacacctc gactcgttac caaataattg cctccaatct
    64441 ccaagtctct accacacgca tcaaaatcaa tatacgtctt caagtgttct ggtacttctc
    64501 caaaatcacc agcttctaca atcaagaaat atgctacatc actcatatca tcgcaatctg
    64561 gataatagat aatatcatcc acatgctcta ccatctcctc aaaagaattg aagaaagcat
    64621 gttgaatctc agaagcaact tcaccaactg gcgttccttc aagttcctcc gctaagccac
    64681 acaaccgatt aatttcctca attggcgtat actcatcaat ctcaaaggga agttcataat
    64741 catgaattgc atattcctcg tattcgtcat ttaacccaat tcgttctttc acttcatcaa
    64801 aatcgacagg tggggtaaac cacgccccca ccaattcacc ttcgttatat ttcccgaggt
    64861 tcgcaatata aacacgcatc tgttccatcg tgcttccccc ttacgcaccg acaattcggt
    64921 taaacaaact gagtaagaca tccttcaccc cagaagcatt aaagaccaat ccgaccgcaa
    64981 tcaaagcaat caccaaaaag ccaatcagtt tggaaaactc ccgcttaaat cctaaaaaca
    65041 aaccaatgac cacaattgcc agtaagacta aggattgggc attggttaaa aaccattgat
    65101 atatggatag gtaagttcct gatattataa atatctcaaa cttttccccc cattcacacg
    65161 gagcgtgcga ctttcaccgc actccgcgtt ccatcgtgtt tatattgaaa agtatttatg
    65221 tagtagacct atatcaagtt taattcttga ttgccagcta atactctata tttatttagt
    65281 cttatgagct ggactttatt tagcttcaat agagctatat atttttgtat tgtttcttct
    65341 gttttagcgt gtattagacg gtgtaccgcc tcttttacca aaataagatt tttataatcg
    65401 tcatttccac cagctccagt tggtacaata tgatgacaat gtatttcatt agcttgttga
    65461 aattcttcca gagttatagc acattttcca tgttgagcag aaaatagcga aattcgattg
    65521 tctgcgtatt ctattgtgcg attttccaaa gataatttca ttaactggta catcagtaat
    65581 ttatcaacac ttagattggt gtggataaat tttcttccct ctagagtgta gcaattcgct
    65641 aaatacacct ttgccattgg ttttctgtgt tggatatatc caacagggaa aataggttcg
    65701 ccctcgccag caacataccg aagcatttta gatttaccat atcttgttgc ctcctttgtc
    65761 gtaagttgtc tgcctgtttt tctcaatctg ccccttttgt ttgtacctaa tctgttagtg
    65821 aagatagcca ttacagctct attcagtttc atacaatcca gatttacatt cgttgcaatc
    65881 tgataataat tttgcatacc taataccatt gaattataaa gtcttgtttc ttctaattcc
    65941 gttttccctt ttgagggaga agcaattttc tttgcctgtg ataccagctt gtctttttgg
    66001 tttttgaatt gtttatcact tatatgtgat ttaacaacta acttctttct tctcggtatc
    66061 attttaatct tgaaaccaag aaagtcggag tatctttttc taacatttac aattcttgtt
    66121 ttttcttgtg atacttccaa ttttaatcgt tgttcaatcc attgagtgat tgctattttg
    66181 gctttttcag cactttcctt gtagcggcag aaaactctaa aatcatctgc ataacgaacc
    66241 atatacattt cctttaactt tgttttcttc atttctttgt aagcgttgct tttacatggg
    66301 ctaccgcttt tattgatatg aaccacatag ttttttgtaa caggattttc ctgccattgg
    66361 ctttctaccc aatggtctaa ctcattaaga acaatgtttg ctagtagtgg ggatattatt
    66421 cccccttgcg gagttccttt ctctggatat gttattgtac cgttagtcat tttgattgga
    66481 gcttttaata tcctttttaa aatgaatatc aagtgcttgt cccgtattcc catagcccat
    66541 atctgtttta tcagcttgga atggttcacg ttgtcaaaga aacccttaat atcaaattca
    66601 atcacataat gtagctggct gatttgtagg cggttatagg ttgcttttat tgcattttca
    66661 actgacctat taggtctaaa accatagctg ttttcactaa atttagcttc gcagattggt
    66721 tctaatactt gttttataca ttgttgtata agcctgtccc acatacaagg aatacctaga
    66781 ggtctggttt taccatttgg ttttggtatt tcttttcgcc tcactggctt aggtcgatac
    66841 ccatgtggac tgcctgcaac aatgtatctt acttttttga caactgtttc tgctggcaat
    66901 ttgccaatat ctttaattat tgtaccgtct gttcctgctg ttttactgcc tgcatttgct
    66961 ttgatatttc gatatgccag taatatattt tctctcgata ggataatatc cataagatga
    67021 gtgaaaatat ctccattctt acttttggca tataaatcat caaaggtgtt ttgcatttcg
    67081 taatattcag cgtgtcttaa atcttctaca cacaatgttt catttttatt tttaggcata
    67141 aggcttcact cctttacttg aaattgagtg agctttttta tcttactcgt aatccttatt
    67201 gaaacaacat aatacttttc taatatatac acgacttgag ggcattcctc cattctcatt
    67261 acaagaattt cttaggttcg ccctctgctt ttcagcatta gttcaactgc ttattgttct
    67321 tcgtcagttt attctcttga tgagaaccca atacctttcc tcgttccgat aatcttatct
    67381 atacatatat atccttaggt cactagctat aagcctgctg gcttgatacc gcctgtaacg
    67441 ttatatggca tttcataacc tatattctta ctttgccaca ccagctacga tttctcgtac
    67501 ctttgcattt ctacaaagtt caaatttaga cccgtacatt cgctgtttcg tcagttcctt
    67561 atagaacatt ctaaccatag atattttata gacccccgac ctgttggata cgctacccac
    67621 ctctggatag ttttcgatag aagatatatg ttcatcaact attacggtat ctttcagccg
    67681 tctttaccga gcttcaaaca ttctgagttt cctcatacaa tgcttgtcgg aggctcaggg
    67741 caggcgtttc agagcgttac ttcatcattc cacctgtcag attatcagtt ctcctaataa
    67801 ccaaactttt tcgttctttt attagtgcct tagcttttca ctaaggaacg agtcgcacat
    67861 aaattttgtc cgaaattcat cttgtcactt tcctttcttt tccattgctc gatccgctgc
    67921 tgcacttgca ttcatcaagc acatcacagt aacaccaagc atactgccac caaataccaa
    67981 aaaaatatct tttacaattc ccatgacgat tcctcttttc atataatcaa attttcaatt
    68041 gccatacttt gttgctccaa aatcttttta tgtcggtctg ataagcgtgc attctgtacc
    68101 atatcctgga tataggttgt ccgattcaat cgatccaact ccttagccat tttccacgta
    68161 ggggcgactt gtcggcctag ccaattcaag gttcgttcaa tggtatacgg ttttggctcg
    68221 gaagttaatt gaatctcttt ccgattccgt ccaataaaac gcccccattg ttcattcaag
    68281 ggccagttgg ttcgcctttt accttctact ttatccgcaa atcgcaagta acgattgata
    68341 atggaaaacg tggtgctctc aatgctacga ccttttaaca aatcttgaat cgcatgaaaa
    68401 gcacggtcat ttttcagtcg aatctcaaaa cgatttttcg tctcagtttg gtctaatgga
    68461 atccctaact tgatatattg ttcatagtct ttttcataca aacagaaata aacctcactc
    68521 ttgagactgc cgatatataa ggtattgccc atgccgtcct tttcatcagc ctttaaaagt
    68581 tccccggaac gataactttt aaaggtacga aacaaggaga tacattcttc cttctgacat
    68641 tttttcgtta gctcaggtat atccaatagc cccactcgat cattgattgc caagtccaag
    68701 cgtttcatca cgccaccggc ctttagacaa tcctcgaaaa agtcgtacca gctacgcttt
    68761 tgagcgagta aaaaagtttc aaattgtcgg caacctcgcc cctttaattc taaaagcaca
    68821 cctttatctt cttcatgaga caacatcacc acaatatcac ccatgacata ctgttcttga
    68881 taggagtaaa aggcataatc ttcatggagc atatactcaa atttcaaatg gagaatttct
    68941 tgaataattt ttcgtgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
    69001 gtcaaaggca aatcgggatt aaagctttcc agttggtcaa aaattttcct catggttttt
    69061 atagatgcgg ttacgccacc attttctatc tgatttaagt acggccgact aagtccacaa
    69121 gccgtagcca atttattttg tgaaacgccg tattcgttgc gtcggtcttt taatagccga
    69181 taatctaaat tccgttgtgc caagttctca cctccaaatt caaggtgtta actttttcaa
    69241 atgagttaac acctatacaa attacttact atctttgata caacaagcat tcttaatggt
    69301 tgcttactta ttttttgtgt ttttgtaccc ccctgttaga tacgggggtg agacatcatg
    69361 gtcggctaac gccgtccatg aacaggcaca gcaccacgga ggatttctgc ttcgcgaatc
    69421 ctccgcggtc agcgcctaac agtcagttgt gttggtttag gttactcatc tgtaatacca
    69481 tttacttcca actctgcttt catttctaac aagtattgac gaatatctgg ttcttttgct
    69541 tggtccgcaa tccgctcaaa ttctgcaatt acttgattta tttcaattcg agcatccgtc
    69601 tcctcattca ttaaatatgg atcgccagcg actttgacac cattcttgcg aatatcaaat
    69661 tttaaccgtc gcaattgttc ttctaattct gggtctgact gaatctttcc aatcacccaa
    69721 taaatttctc caatcaactc atcataaacc atgtacatcc aactctttta atcttctggc
    69781 aatatcaaaa atgaccggaa ctgttacaga gtttccggct tgcttataca actgactatc
    69841 tgaattgact tgagaatctc gatcaaaagc ccaatcggga aagccctgaa gtcgccaaca
    69901 ctctctaggt gttagcttcc gaatttgata ctgatccgtg acgatcgctt gttggtctcc
    69961 tgttagtaag gtatgaacac tctgttttcc cagacgacct cggcgacttg tggaattagg
    70021 aaaagccaga ttcacactgt cttctggctt cacctcttga tattctgttt cattttttcg
    70081 aatcatgatt tttgtaccac cttgtttcgt tgacaaggtg ggggcggttc cagtaacttc
    70141 ataaactcga ttaccttgtt caaactttcc cggtaatagt cctgctacag agattccatc
    70201 ccttgctggc agtgcgattt tgattccttc atcacctgtt aaggttggcg ctacgccatc
    70261 tgaaccatag acttgtccac ccattcctcg attggaagga ttgatattta ccaaatttct
    70321 gatgttttga tcagatgttg aagacggtcc aatgatagga aatacttgtt cggtacatgc
    70381 gtctcgagaa tgtccgataa ggaatatccg ctcccggctt tggggaatgt aggcctttga
    70441 gttaagcacc taccactcca cgtcataccc caatccatcc agcgttcgga ggatcgtctc
    70501 gaacgtagcc cctccttcgt gattgagcaa tcctcggacg ttctcaagga ataaaagctt
    70561 aggtcggaga agagtggtga accgagcgat ttcaaagaat aaagtacctc gagtatcgga
    70621 aaatctttgc cgctttcccg caattgaaaa agcttggcac ggaaatccgc cgcaaaggat
    70681 gtccactggt ccgagggatt gaataaactc atctgataca cttgtgatgt catgcatttc
    70741 cacctcctta cttgtatcat gaattgcttt ataactacgt ctagcaaatt tatcaatttc
    70801 acaaaaaccg atacaatgat ggctcgcctg ttccatacct aaacgaaagc caccaatacc
    70861 ggcgaataaa tctaaaaatc tcatacatta cctcattcat tgcaaagaaa aaagccaaga
    70921 acattttgct cttggctcat acattattaa tt
//
LOCUS       DAHUVV010000005        54706 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.005, whole genome shotgun sequence.
ACCESSION   DAHUVV010000005 DAHUVV010000000
VERSION     DAHUVV010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.005"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="NK767_000321"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="NK767_000321"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCH6693340.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="NK767_000322"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="NK767_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCH6693341.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="NK767_000323"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="NK767_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCH6693342.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="NK767_000324"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="NK767_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCH6693343.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="NK767_000325"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="NK767_000325"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCH6693344.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="NK767_000326"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="NK767_000326"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCH6693345.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="NK767_000327"
     CDS             complement(8053..8523)
                     /locus_tag="NK767_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCH6693346.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="NK767_000328"
     CDS             complement(8569..8994)
                     /locus_tag="NK767_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCH6693347.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="NK767_000329"
     CDS             complement(9061..9330)
                     /locus_tag="NK767_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCH6693348.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="NK767_000330"
     CDS             complement(9341..10936)
                     /locus_tag="NK767_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCH6693349.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="NK767_000331"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="NK767_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCH6693350.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="NK767_000332"
     CDS             complement(14489..15049)
                     /locus_tag="NK767_000332"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCH6693351.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="NK767_000333"
     CDS             15402..16376
                     /locus_tag="NK767_000333"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCH6693352.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="NK767_000334"
     CDS             16546..17946
                     /locus_tag="NK767_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HCH6693353.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="NK767_000335"
     CDS             complement(18024..18746)
                     /locus_tag="NK767_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6693354.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="NK767_000336"
     CDS             complement(18825..19331)
                     /locus_tag="NK767_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCH6693355.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="NK767_000337"
     CDS             19454..21253
                     /locus_tag="NK767_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HCH6693356.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="NK767_000338"
     CDS             complement(21321..22895)
                     /locus_tag="NK767_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCH6693357.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="NK767_000339"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="NK767_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCH6693358.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="NK767_000340"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="NK767_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCH6693359.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="NK767_000341"
     CDS             complement(24672..25310)
                     /locus_tag="NK767_000341"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCH6693360.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="NK767_000342"
     CDS             complement(25311..25961)
                     /locus_tag="NK767_000342"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCH6693361.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="NK767_000343"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="NK767_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCH6693362.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="NK767_000344"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="NK767_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HCH6693363.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="NK767_000345"
     CDS             complement(29104..29844)
                     /locus_tag="NK767_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HCH6693364.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="NK767_000346"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="NK767_000346"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCH6693365.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="NK767_000347"
     CDS             complement(31215..32153)
                     /locus_tag="NK767_000347"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCH6693366.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="NK767_000348"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="NK767_000348"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCH6693367.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="NK767_000349"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="NK767_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCH6693368.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="NK767_000350"
     CDS             complement(35313..35618)
                     /locus_tag="NK767_000350"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCH6693369.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="NK767_000351"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="NK767_000351"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCH6693370.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="NK767_000352"
     CDS             complement(36724..37008)
                     /locus_tag="NK767_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693371.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="NK767_000353"
     CDS             complement(37026..37916)
                     /locus_tag="NK767_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCH6693372.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="NK767_000354"
     CDS             complement(37936..38154)
                     /locus_tag="NK767_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HCH6693373.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="NK767_000355"
     CDS             complement(38124..39824)
                     /locus_tag="NK767_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCH6693374.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="NK767_000356"
     CDS             40091..40864
                     /locus_tag="NK767_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693375.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="NK767_000357"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="NK767_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HCH6693376.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="NK767_000358"
     CDS             complement(41688..42659)
                     /locus_tag="NK767_000358"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCH6693377.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="NK767_000359"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="NK767_000359"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCH6693378.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="NK767_000360"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="NK767_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCH6693379.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="NK767_000361"
     CDS             complement(45614..46438)
                     /locus_tag="NK767_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCH6693380.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="NK767_000362"
     CDS             complement(46411..47091)
                     /locus_tag="NK767_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCH6693381.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="NK767_000363"
     CDS             complement(47114..48052)
                     /locus_tag="NK767_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCH6693382.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49160)
                     /locus_tag="NK767_000364"
     CDS             complement(48297..49160)
                     /locus_tag="NK767_000364"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCH6693383.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="NK767_000365"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="NK767_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCH6693384.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="NK767_000366"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="NK767_000366"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCH6693385.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="NK767_000367"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="NK767_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCH6693386.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT        15117 a        11891 c         9818 g        17880 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctggcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tggaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgaga
//
LOCUS       DAHUVV010000006        54454 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.006, whole genome shotgun sequence.
ACCESSION   DAHUVV010000006 DAHUVV010000000
VERSION     DAHUVV010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.006"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..548)
                     /locus_tag="NK767_000368"
     CDS             complement(<1..548)
                     /locus_tag="NK767_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCH6693387.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKI"
     gene            complement(625..1155)
                     /locus_tag="NK767_000369"
     CDS             complement(625..1155)
                     /locus_tag="NK767_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCH6693388.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1159..2019)
                     /gene="rlmB"
                     /locus_tag="NK767_000370"
     CDS             complement(1159..2019)
                     /gene="rlmB"
                     /locus_tag="NK767_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCH6693389.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2012..2407)
                     /locus_tag="NK767_000371"
     CDS             complement(2012..2407)
                     /locus_tag="NK767_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCH6693390.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2404..3816)
                     /locus_tag="NK767_000372"
     CDS             complement(2404..3816)
                     /locus_tag="NK767_000372"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCH6693391.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3813..4349)
                     /gene="cysE"
                     /locus_tag="NK767_000373"
     CDS             complement(3813..4349)
                     /gene="cysE"
                     /locus_tag="NK767_000373"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCH6693392.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4435..4674)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4671..6131)
                     /locus_tag="NK767_000374"
     CDS             complement(4671..6131)
                     /locus_tag="NK767_000374"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCH6693393.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPKMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6301..7458)
                     /locus_tag="NK767_000375"
     CDS             complement(6301..7458)
                     /locus_tag="NK767_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCH6693394.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7492..8862)
                     /gene="radA"
                     /locus_tag="NK767_000376"
     CDS             complement(7492..8862)
                     /gene="radA"
                     /locus_tag="NK767_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCH6693395.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8925..9452)
                     /locus_tag="NK767_000377"
     CDS             complement(8925..9452)
                     /locus_tag="NK767_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCH6693396.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9682..10488
                     /gene="proB"
                     /locus_tag="NK767_000378"
     CDS             9682..10488
                     /gene="proB"
                     /locus_tag="NK767_000378"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCH6693397.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10485..11732
                     /locus_tag="NK767_000379"
     CDS             10485..11732
                     /locus_tag="NK767_000379"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCH6693398.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11808..12152
                     /locus_tag="NK767_000380"
     CDS             11808..12152
                     /locus_tag="NK767_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693399.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12269..12595)
                     /locus_tag="NK767_000381"
     CDS             complement(12269..12595)
                     /locus_tag="NK767_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693400.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13210..14412)
                     /locus_tag="NK767_000382"
     CDS             complement(13210..14412)
                     /locus_tag="NK767_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6693401.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14529..15044
                     /locus_tag="NK767_000383"
     CDS             14529..15044
                     /locus_tag="NK767_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCH6693402.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15243..15689)
                     /locus_tag="NK767_000384"
     CDS             complement(15243..15689)
                     /locus_tag="NK767_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCH6693403.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15736..17214)
                     /gene="msr(C)"
                     /locus_tag="NK767_000385"
     CDS             complement(15736..17214)
                     /gene="msr(C)"
                     /locus_tag="NK767_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCH6693404.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17568..18197)
                     /locus_tag="NK767_000386"
     CDS             complement(17568..18197)
                     /locus_tag="NK767_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCH6693405.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18181..18645)
                     /locus_tag="NK767_000387"
     CDS             complement(18181..18645)
                     /locus_tag="NK767_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCH6693406.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19132..20400)
                     /locus_tag="NK767_000388"
     CDS             complement(19132..20400)
                     /locus_tag="NK767_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCH6693407.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20631..21368)
                     /locus_tag="NK767_000389"
     CDS             complement(20631..21368)
                     /locus_tag="NK767_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCH6693408.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21435..22003)
                     /locus_tag="NK767_000390"
                     /pseudo
     CDS             complement(21435..22003)
                     /locus_tag="NK767_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            22131..23003
                     /locus_tag="NK767_000391"
     CDS             22131..23003
                     /locus_tag="NK767_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCH6693409.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23345..24217
                     /locus_tag="NK767_000392"
     CDS             23345..24217
                     /locus_tag="NK767_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCH6693410.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24214..26172
                     /locus_tag="NK767_000393"
     CDS             24214..26172
                     /locus_tag="NK767_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCH6693411.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26504..27355)
                     /locus_tag="NK767_000394"
     CDS             complement(26504..27355)
                     /locus_tag="NK767_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCH6693412.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27478..27945
                     /locus_tag="NK767_000395"
     CDS             27478..27945
                     /locus_tag="NK767_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCH6693413.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28208..28690)
                     /locus_tag="NK767_000396"
     CDS             complement(28208..28690)
                     /locus_tag="NK767_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCH6693414.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28790..29653)
                     /locus_tag="NK767_000397"
     CDS             complement(28790..29653)
                     /locus_tag="NK767_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCH6693415.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            30251..31126
                     /locus_tag="NK767_000398"
     CDS             30251..31126
                     /locus_tag="NK767_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693416.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(31271..31423)
                     /locus_tag="NK767_000399"
                     /pseudo
     CDS             complement(31271..31423)
                     /locus_tag="NK767_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(31691..33022)
                     /locus_tag="NK767_000400"
     CDS             complement(31691..33022)
                     /locus_tag="NK767_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6693417.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(33171..33884)
                     /locus_tag="NK767_000401"
     CDS             complement(33171..33884)
                     /locus_tag="NK767_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCH6693418.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            34059..35483
                     /locus_tag="NK767_000402"
     CDS             34059..35483
                     /locus_tag="NK767_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCH6693419.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            35782..36489
                     /locus_tag="NK767_000403"
     CDS             35782..36489
                     /locus_tag="NK767_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCH6693420.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            36476..36799
                     /locus_tag="NK767_000404"
     CDS             36476..36799
                     /locus_tag="NK767_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCH6693421.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(36888..37925)
                     /locus_tag="NK767_000405"
     CDS             complement(36888..37925)
                     /locus_tag="NK767_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCH6693422.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(38246..39229)
                     /locus_tag="NK767_000406"
     CDS             complement(38246..39229)
                     /locus_tag="NK767_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCH6693423.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(39644..40414)
                     /locus_tag="NK767_000407"
     CDS             complement(39644..40414)
                     /locus_tag="NK767_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6693424.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(40407..41342)
                     /locus_tag="NK767_000408"
     CDS             complement(40407..41342)
                     /locus_tag="NK767_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6693425.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(41658..43307)
                     /locus_tag="NK767_000409"
     CDS             complement(41658..43307)
                     /locus_tag="NK767_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCH6693426.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(43424..46027)
                     /locus_tag="NK767_000410"
     CDS             complement(43424..46027)
                     /locus_tag="NK767_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCH6693427.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(46039..47088)
                     /locus_tag="NK767_000411"
     CDS             complement(46039..47088)
                     /locus_tag="NK767_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCH6693428.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(47114..47428)
                     /locus_tag="NK767_000412"
     CDS             complement(47114..47428)
                     /locus_tag="NK767_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCH6693429.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(47444..47890)
                     /locus_tag="NK767_000413"
     CDS             complement(47444..47890)
                     /locus_tag="NK767_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCH6693430.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(47891..49813)
                     /locus_tag="NK767_000414"
     CDS             complement(47891..49813)
                     /locus_tag="NK767_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCH6693431.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(50025..50921)
                     /locus_tag="NK767_000415"
     CDS             complement(50025..50921)
                     /locus_tag="NK767_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCH6693432.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(50914..51594)
                     /locus_tag="NK767_000416"
     CDS             complement(50914..51594)
                     /locus_tag="NK767_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6693433.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(51595..52377)
                     /locus_tag="NK767_000417"
     CDS             complement(51595..52377)
                     /locus_tag="NK767_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6693434.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(52370..53287)
                     /locus_tag="NK767_000418"
     CDS             complement(52370..53287)
                     /locus_tag="NK767_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6693435.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(53451..53774)
                     /locus_tag="NK767_000419"
     CDS             complement(53451..53774)
                     /locus_tag="NK767_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693436.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT        15980 a        11300 c         9457 g        17717 t
ORIGIN      
        1 tttattttga gttttactct cccgtaagcg ctcattactt gcttgtgatc cattgctaaa
       61 cattcagcca tgtctacaaa atcaacgcct tgcatataat aatgaaaaat tttagcttct
      121 atttttgaaa actggatttc gcaattttcg attgcttctt ttaataaaag atgctgaatt
      181 gctaaaggtt cttgcaatgt gatctctgct aaaaagtctg gtccttctgt ctctattttt
      241 tggtcgattg agacttcatc tttttgatca cgacgcttca gcgcgttctg cttcctcaac
      301 agagaataga tttgattgct tataacgctt ttataataac ttccaaagct aacacctttg
      361 cttttttcgt aagttctcaa tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc
      421 tcagaaacat aatttcttaa ataataggtg tttttcaaac gtagtaaaag tggaaaatat
      481 ttttgataca gggcagcaaa tgcttcttcg tcaccattac gtgcagcgcg caataactcc
      541 tcagacataa aataattcct ccataaattc gtttatggag gaatcttatc atcaatgatt
      601 catcgaactg attagaacga tatgttattt attttttctc attaaatcgt ccaatttttg
      661 ggacaatttt tttaactgct cttcattcca tggagaatta cgtcggaaat caataaaatg
      721 catatcttta ttttcttggg ctattgtttt ttccgttttt tttatcgtct tatataattc
      781 ataagcagaa gttcgtaatg ctccttggga aaagatcatc cactgttctg ctaaatcgct
      841 tgtcgccact gtcacttgtg tcaaacggtc attcagctga ccagcgattc tctcgatata
      901 actgtcagcc gtttcgtctt ctttcgtaaa aacaacatcc aactggtatt tcttataatt
      961 ttgttggatg cccggaacta gttgggcatc gaagacaacg atgacttcca ttccttcata
     1021 tttcgcataa tttgataaac gatgtaaaag agcttctctt gcatccgcca ttttgtcctg
     1081 ctttttaagc tggacaagtt ccggccacga accaatcatg ttgtagccgt ctacaatcaa
     1141 cagctgtttt ttcatggtct actccccctg acgttgggaa aaagcctgat acataagcaa
     1201 accagcagca acactggcat ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa
     1261 ttcatccatt tcttttttca atcctggact cattcctttg ccttcattac caatgactaa
     1321 agcaattgat cctttggcat tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa
     1381 tacccaaaat cctgtttctt tgagctgttg cacagcttgt accaaattcg tcacgcgtgc
     1441 aacagggaca tgttcgactg ccccagtgga agtctttgtt acgataggtg tgattccaac
     1501 tgctcggtgt tttggtatga tcacaccatc gacgccactt gcgtctgctg tccgtaaaat
     1561 cgacccaaaa ttgtgtggat cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt
     1621 tttctctttt gttcgttcaa tcagttggtc aagggataaa tattcataag gtgtaatcgc
     1681 aagaacgact ccttgatgca cgccgtgatc gctcaaagtt tctaattttt gtttaggaac
     1741 ccatttgact ggcacagctt gttcacgtgc gatttctttc agttcttcta ctttttctcc
     1801 tcgtgaatct tcttgaagaa agagtttatt ccctcgccct tgttgtagcg cttctactgc
     1861 ggcatggtgc ccaaagacga aattatcttc taatgcttct tcattttgat cagaaactga
     1921 ctgatttcgt ccagtttgct ttttatttga atatctattt tgttttttgt cttcaaatcc
     1981 acgtttcgat tgatgtttgt ttgttctttc tttacccatt tttttcacca actttctgaa
     2041 tacaccagcg gatcaattct tccatccgct cggtttgttt tgtcaaatgc agatagccca
     2101 tgagcgcttc gaaccctgtt gcgacccgat acgtcgtaac atcagcattt tttgcgctcg
     2161 tatggctttt agcatttctt cctctgcgat aataaagttc ttcttcttct gtcaaaagat
     2221 gttcttccag catttgctga atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg
     2281 ccgcacgatg cagcttattg ggttttgttt ctccttgatc aatcaggtgg tcacgaacat
     2341 agatttcgta aatcgcatct ccaacataag ccaaggctaa accgttcaat tgtgtgtaat
     2401 ctctcatgct tctcttctcc atcgggttcc ttgcggtgta tcttctaata agatcccttt
     2461 ttctttcaac aaatcacgaa tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg
     2521 atttcgttct tcgatcaatt gttcaatctc ttcatctaat aactcttctg ctgtaaataa
     2581 aataccgaag atcgaaagcc attgcgagaa taaagcttct ccttgtgcca agacagcggc
     2641 agaaactttc ggctggtcta gataagtgtt cagccactta gcaagctcat agaccactgt
     2701 gatcccattt gctgcattaa aatcatcgtc catttcttcg atgaatcgct gttctaaatt
     2761 ttctaacaaa gcgattttat ccgcatcatc tgccaaattc tctgaggcag tttcttggcg
     2821 gaatttcaaa ttgtcaaacg tattcttcag tcgttgtaaa ttggtggctg cttctttcat
     2881 cgtcgtttca ctgtagcgga tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa
     2941 gacttgtgga tcaacgtttt tgatcatttc atgtacagtg atgaaattcc ctagagattt
     3001 actcattttt tcatcgtctt cgccaatcgt tacatagcca ttgtgcatcc agtaattagc
     3061 gaattttttc cctgttttcg cttcactttg tgcgatttca ttttcatgat gaggaaattc
     3121 taaatcttgc ccgccaccat ggatatcgat cgtttctcct aaatgttttg ttgccataac
     3181 ggaacattca atatgccaac cgggacgtcc tttgccccat ggtgaatccc aagaaatctc
     3241 gccaggtttt gcttgtttcc ataaagcgaa atctaatggg tcttcttttt gctgctgttc
     3301 ttcccctgta cgctgacttg ctccgacttc cagttcatcg atggattggt cacttaattt
     3361 cccataattt tcaaatttac gggtacggta atacacatca cctgaagatt cataagcata
     3421 gcctttttca accaacactt gaataaatgc taaaatatct ggcatatgat ccatgactct
     3481 tggatgcagt gtagcaggtt cgacattcaa cgctttcgta tcttcttcaa atgctcggat
     3541 gaaacgatca gcaacttctg gtgctgtaat acctaattcg tttgctgtac ggatgatttt
     3601 atcgtctaca tccgtgaaat tcgatacgaa atcgacttta tagcctcgat attcaaaata
     3661 gcgtcgaatt gtatcgaaag caatggtgct acgagcattt ccgatatgga tataattata
     3721 cacagtcggt ccgcagacat acataccgac atgtcccttt tgtataggga tgaattcttc
     3781 tttttttctg gtcaatgtat tgtagatttt tatcattctg atctcctcac tttttctcct
     3841 ttgattcgaa cgacttttgc tggtactcct accgctgtag cgtcgtctgg aacatctttc
     3901 aacacaacgg cacctgcacc gattttggcg ttttttccga tcgttaccgg tcctaatatc
     3961 tgcgcattcg cagagagcat ggctccttgt ttaacagttg ggtgtctttt acccgtttct
     4021 tttcctgtgc cgcctaatgt tacgccatgg aataaaatca catcgtcctc gatttctgcg
     4081 gtttcgccaa tgacgattcc cattccatgg tcgatgaaca cgccagttcc aatcgttgct
     4141 cctggatgga tctcaacacc agttaggaaa cgccagaatt gagcattgat cttcgccaat
     4201 aaaaagagcc ggtgctgata taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa
     4261 ccagggtatg tcaaaacgat ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct
     4321 gcatcaatcg ctcgggtaaa ccaactcatg actttccact cctttcgtaa gaattttatg
     4381 taggaaatca tcttcttata aagaaaagaa gcgcctttta aataagtttc cttatcaaaa
     4441 gacgctttcc gcgtggttcc acttttcttc ataatcaatg attatctctg acagctaacg
     4501 cctgttatac gttcttggct actttgtttc ctataaaaca gtttttcctg ttttctgtgt
     4561 ttcgccaaga ccactcccag aggcatttcg gcaaccattc attgatacct tgcaccaccc
     4621 ggtatctctc ttgtaatgat cagctgctta cttttttctg ttcatcgtgt ttagttcagt
     4681 acttgattta aatgtgctaa tgatttttca cgaccaagca attcgatcgt ttcaggcaat
     4741 tctggaccat gcatttgtcc tgatacagct acacggattg gcatgaaaag attttttcct
     4801 tttacgccag tttctttttg aacagctttg atggcagctt tgacagttgg agcatctact
     4861 acttccattt gttctagctg tgctttgaat gcgtggagaa cagtaggaac tgtttcacca
     4921 gcaagtactt cttttgctgc ttcattcaat acaggatgtt cattgaaaaa tagcgtagac
     4981 agatcaacga tttctgcagc atagctcatt ttaggttgat acaggctaac gatcttcttc
     5041 acccattcga ttgtcttttc atctgggttt tctgctacac gtccatcagc gattagataa
     5101 ggcagacaca tgtcagtaaa tgcatctaga tccattgctt tgatgtaatg gttgtttacc
     5161 cattgcagtt ttttcgcatc gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac
     5221 attttgatca attcttggtg ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat
     5281 aatgcaataa agttgaacat tgcatctggt agatagccta attcgcgata ttgttcgata
     5341 aattgcaaga ttgattcgtc acgtttgctt agttttttac ctgtttcact gttgatgatc
     5401 aatgtcatat gtccgaaggt tggtggtgtc cagccaaatg cttcatagat catcagttgt
     5461 tttggtgtat tggcaatatg gtcatctcca cgtaaaacgt gagtgatttt catcaaatga
     5521 tcatcgaccg caacagcaaa attataagtt ggcataccat cgcgtttttg gatgacaaag
     5581 tcgccaccga tattatcgga ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg
     5641 tattctgtat tgcgaggcac acggaaacgt acaacagatt ctagaccttg tgcttctttt
     5701 tctgcttgtt cttcaggtgt caagttcgca catgttcctg cgtaatgagg catttctcct
     5761 cttgcgcgtt gtgcttcccg ttctgcttct aattcttctt ctgtgcaata gcatttgtat
     5821 gctcgattgc tagcaagcag ctgatcaatc agcggttgat agatttcttt acgttcagat
     5881 tgacggtaag ggccgtattc tccaggattt tcaggagatt catcccaatt cattcccaac
     5941 caagcaaggt tttccaactg gcttttttcg ccatcttcga tattacgttt ttggtcagta
     6001 tcctcaatac gaataataaa gtctccatca ttgtggcggg caaacaaata attgaataat
     6061 gcagttcttg catttccaat atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt
     6121 actttcgtca ttttcttcct cttctttcaa actagacttt gactaacagc aaaaattccg
     6181 tcagttacgg cttctcaaat atcatttatt taaagccact cagcacaaat tttacctttt
     6241 ctaaggtaaa tagtaaagcc aactggttga atttccttac cagttggctg aatttctttt
     6301 ttattttgat tgtttagtct ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc
     6361 atgtgcaggt ttagcaaaga tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac
     6421 gacttcgata cgttcattca tgtaatgctg tccatcttct acgacaacca tcgtgccgtc
     6481 atcaaggtag gctactcctt gttggcgttc tgtcccggct tttactacta gaacattcat
     6541 cgtttcacca gggatgacga ctggttttac ggcatttgcc aacgcattga tattaagaac
     6601 aggtacattt tggaattcag agactttatt caagttatag tcattggtta caatgacacc
     6661 atctaaaagc ttagccaatt tgatcaattt gctgtctact tcactgatat cttcaaagtc
     6721 tccatcgtac atttcgacag atataccctc ttctttttgc aaggcattta ggatatctaa
     6781 tccgcgacga ccacgcacac gtttcaaact atcccctgag tccgcgatgt attgtagttc
     6841 gtaaagaaca aaattaggga tcatcaaggt tccttctaag aatccagtct ttgtaatatc
     6901 ataaatccga ccgtcaatga tcacgcttgt atccaagatt ttatatttat ggaaattttc
     6961 tcctgatttt cgttctaaca gttgactctc aacttgacta gctgtttctt gctcgatttt
     7021 tttgcttcgt gaaccaaaaa ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa
     7081 tcccagatag cctaagatga tcatcagtaa aatcggcaat acactattga cgaatggaat
     7141 ttccatattg taaagagggg tcgagatcac aacacctaaa gtcaatccga tgatcgcacc
     7201 gatcgcaccg aagagaagat aagtcaagct gacctcactt agtttttgtt ctacttgttt
     7261 gataatagct gagatatgtt tggccaacag caaagataat aaaaagaaaa taagtgcgcc
     7321 taacaggctg ttggtaaatg tattattcag ccatccgttt gactgttgac cgattgcctg
     7381 ccaagctgag ggtaaaaatg aaatacccaa gcttgcgccg acaatgatca tcagcaaagt
     7441 aatgacacgt ttttgcatcg tttttcctcc gtttcgacaa tcatttatta gttagcggaa
     7501 cacacgcttc aacgtttcag ccaaagttgc tactccaact atctcgattc cttttggcgc
     7561 ttcccagtta cctaagttgt ttttaggtag gtatactttt gtgaagccga gtttttggac
     7621 ttctttgact cgttgttcaa tatgactgac acgtctgatt tctccagtca gtccaatttc
     7681 accgatgaaa cattccgttg gctgtgttcc tttttcttta tagcttgaag caatactgac
     7741 agcgatagct aaatcaatcg ctggttcatt taattttacc ccacctgcag cttttaagta
     7801 agcatcttga ttctgaagaa gcaacccagc tcttttttcc agcactgcca tgatcagaga
     7861 tacccgattg aaatcaagac ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt
     7921 gactaatgct tggatctcta ccaaaattgg acgtgtccct tccattgcca caacgatagc
     7981 agagccagtt gcaccgtcta ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc
     8041 ttccaatcca tgctcatgca tttcaaaaat cccaatctca ttcgttgagc caaatcggtt
     8101 ttttactgct cgcaaaatcc gaaaactatg atgttgttca ccttcaaaat ataacaccgt
     8161 atccaccata tgttctaaca ttcttgggcc ggcgatcgag ccttctttgg taacatgacc
     8221 gacgataaaa atggctatcc catttgtttt agcggttttg agtagttctg cagtggtttc
     8281 tcttacttgg ctgacacttc ctgccacact tgtgatgtct ggctgcgtca tcgtttggat
     8341 ggaatcaatg ataacataat caggggagat atgttcgatg gcacgactga tttcgttcat
     8401 atctgtttca gcgtatagat aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa
     8461 ttttatctgc tctgcgcttt cttcgccgga tacatataag acttttccgc ctatggctgc
     8521 taattgctga gaaacttgta acagcaaagt ggatttcccg atacctggat cgccaccaat
     8581 caagaccata gaccctggca ccacacctcc gcctaacaca cggttcagtt cttctaattt
     8641 tgttttgatc cgaggttctt ttttagggac gacgtctgcg atttttgttg gttttgcttt
     8701 ttctcctgtc aaacttgtcc gagttctgcg gtccgttgta tcttgttcga tttcttcgac
     8761 catcgtattc cattttccgc aatttgggca gcgtcccaag aacttaggcg acacataccc
     8821 gcaattttgg caaacaaatt gtacttttga tttttttgcc attgtaccgc ctccttttat
     8881 cctaatcatg agacattaaa tcaagcttaa gtatctttaa cgttttattt actggaagaa
     8941 ccaaatccgc cttgacgttg agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa
     9001 aagatccctt ggccgatacg ctcgcctttt ttgatcacta catccttgaa accaaagttg
     9061 gtgaattgga acatgatatg gccttcattt gcctcattat tgtaataatc actgtcaatc
     9121 acacctactc cgtttgtcaa cactaaaaac cgcttcaacg gattgcttga acggttagca
     9181 agctgcaaaa attcatcttc tcccatgtaa gctttgattc cagtaggcac gagggttggt
     9241 ttcaattgtt tttcattttc tgatacatct tttttcaatc catctgcgac tgtttccaac
     9301 ccctttttcc agcctgcttt ccagatactt ggaatcacga cctcttctgc tgcttcaaaa
     9361 tcatagccag cagcatgatg tgtagcgcgc tgcggtagat tgatattttg atctttatat
     9421 ttagaaataa cttcaaatcc tctttttctc accggttttc ctccctaaat aaactactcc
     9481 tattctaaca tatccgaggc tgtaaaagac tttctttaca aattcttcaa gaaattctgc
     9541 tatcttaaag taaaccatta attcgttctt tttccaaacg actgcaagtt gtacaaaaaa
     9601 actatttttt acatgacttt actcgtcaaa acaagtagaa tagaacgaaa ttaatgagtg
     9661 gatatttgat tgaggtgtca catgcgtaaa gaaattaaaa atgcaaaacg aattattgtc
     9721 aaagtaggaa caagtacatt gatttatccc aatggaaata tcaatcttgc ggcaatcgat
     9781 cagttagctt ttgtcctttc tgatcttaga aatcggggaa aggaagtgat cttagtctca
     9841 tcaggagctg taggcgttgg tatgcataaa ttacatttgt cagaacgtcc gactttgatt
     9901 cctgaacagc aagctgcggc ggctgttggt caagccgaac tgatgaatat ctataatcaa
     9961 agattcagtg tatataatca acagacggca cagatcctcg tgacaagaga cgtggtagat
    10021 tatcctgaaa gtcgtaaaaa tgtgaggaat acattggaac agcttttgat tatggacacg
    10081 atccctatcg tgaacgaaaa cgatactgta gcagtggatg aattagatca tctgacaaaa
    10141 tttggcgata atgatcagct gtcagccatc atggcaaaac ttgtcgcggc agaccttttg
    10201 atcgtccttt ctgatatcga cgggttttat tcagataatc cgactacaaa tcccgaggct
    10261 cagatgtatc gctatatcga taaaatcgat gaaaagattc tggcacaagc aggtggtgct
    10321 ggtagcacat ttggaacagg cggtatgcag agtaaattaa aagccgcttc taggatattt
    10381 gaagcaaaca gcgcaatgat tttagcaaat ggaaaaaatc cggcaattat ttttgatatt
    10441 cttgcaggaa aagaagtagg tactttattt aaggaggact tgaaatgact gacttgatgc
    10501 aattaggaca acaggcaaaa gatagcgcgc aacaactagc acttatgcct acaaaccgca
    10561 aaaatgagct acttgttcaa atggcacaaa ccatcaaaaa gaatcaacaa gcgatcattg
    10621 aagcaaatca taaagactta gaaaaagcag tcgaaaacaa tatcagtgaa acgatgcaag
    10681 accgcttgca gctgacacct gagcgaatca atgccatggc tacagagatc gaaaaaattg
    10741 cttcgttaga cgatcctatt ggaaaagtag atgagatgtg gacgaacacc gacggattga
    10801 gaatcggcaa aaagcgcgtg ccactaggcg tgatcggcat catttacgaa tctcgtccaa
    10861 atgtcacgac agatgcagct agtctagcat tcaaatcagg gaatgccgtt atccttcgcg
    10921 gtggcaaaga agcattcttc tcgaatcaat tattagtcca attattgcag caagtactgg
    10981 tcagtgctgg cgaatctcct tatgccatcc agtttgtcga tgatacttct catgaaactg
    11041 caggacagtt gatgcgattg acagaatact tagatgtgct gattcctcga ggcggagcaa
    11101 aattaatcca acgagtcaaa gaacaggcaa cggtacctat cattgaaaca ggtacaggaa
    11161 ataatcacgt atatatcgat aaagaagcac agttatctat ggcagtgaac atcatcgtca
    11221 atgcaaaagc ttcccgtcca tctgtctgta acgcggcaga aacattgctg atccattctg
    11281 agatcgcacc ttttttcttg ccagctattg aaaaagaact ggtagaacac ggggtctctt
    11341 tacgtgcgga tgctcgtgcg ttagaatatc tagagacggc tgctttagca gaagacacag
    11401 attgggatac tgaatatcta gattatatcc ttgctgtcaa agtggtcgat tctttggatg
    11461 aagcaattgc acatatcaat cggcataata ctaaacattc agaaacgatc gtgacagata
    11521 gttatgcggc tagtcaacgt ttcttaaatg aagtggacgc ggcggctgtc tatgtcaatg
    11581 cctccactcg ttttacagac ggatcggtat ttggttttgg tgcagagatt ggtatctcta
    11641 ctcaaaagtt gcatgcccgt ggtccaatgg ggttaaatga attaacttcc acaaaatata
    11701 tcatttatgg cgatggacaa atccgcgaat aatgtttcgt gtgaaacatt attcccgcct
    11761 tttgcctttt ttctgtaaag gttctatcca actagaaagg tgtcaatatg tttgaaattt
    11821 taatgatttt ttttatttac cttatttctc tgaatatcgc tgcttttctg ggagtcggta
    11881 ttttatccct tttctttcaa ttcaaaaaac gaagcatagg gagtcaacgg gaaaaatggt
    11941 cgcagtattt tgacaaaatc gggcaaaaag ggctggttac acgcttgcac attagttaca
    12001 tggttgcatt atgcttatta gcgacaatca attactattc ttttttcgat cattcaatcg
    12061 cttatacgat tacgctactg attgctggga tttttcactt atcttataaa tatcagctga
    12121 ataaaaacca cttgaatcgg actttccgct aaaaaatatc cgtacgataa gtagagtgcc
    12181 ttgaagatcg gcaaaaaact gccggacttt ttagaggctg tgacaaagat tttgtcacag
    12241 cctctttttc catataaacg gcgttcgcct accagctgcc tggtaaatct gtttcctctt
    12301 cgttaagaag cgaaaatccg tcaaatgttt cgccatcata ataacgaagc agctcatcca
    12361 tggacaagtt catcgttttt tctttttctt cttccgctga ttcagccagt agctgttccc
    12421 gatacacctg atacatacca gctatttttt gctgcatcgc ttttctagtt tcaccttccg
    12481 cattgattat tggcaattct ttgatatagc cacgataaat caaccgatca tctgaaaatt
    12541 caatgggtaa ttcaacgagt gtcaacccga cttcctgaaa atattcttcc agcatctatt
    12601 atccccccat tctttttctt aaatgaattt tagcttacct actgaaaatt ttccagtttc
    12661 attattttga gaaaaaaagg gaatgtgaca aaaatcgtgt cacatcccct ttttccgaat
    12721 aaatggtgtt cttctgccaa ctcctcaatc ataagtcaca atattcaaca aacatgagaa
    12781 actgtttttt gaaattgttc cttgtgattc agagttaaac gacagtctca caacctcttg
    12841 gtaaacggcg ttcaatcagc tgaccctcgg tattagctca aaaaacggta aaatatgaga
    12901 agctattttt ccgttttgtt tctctaatac ttaggataaa acgctgattt tacaacctct
    12961 gttaaacggt gttcaatcag ctgtccttcg gtattagctc aaaaaacggc aaaatatgag
    13021 gaactatttt tccgtttgtt tctctaatac tcaggacaaa acgctgattt tacaacctcc
    13081 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
    13141 gagaagctat ttttgtttgg ctgttcttat gacttgatgc aggacagctt tttcacaacc
    13201 tctgatttct tattcttctt catttgtagc aaaataatta cggtgataaa tgatacaaca
    13261 tacactgata agaacaatgg cacctgcaaa ataaaacgca tagctttcac caatatgttc
    13321 accaatcaca cctgcaataa atggtgctaa agctccaccg aaaaagcgga caaaactata
    13381 gctggacgaa gcaacgtttc gttgcagacc tggtatttcc atagcaatcg tcgtcaacag
    13441 tgtattgata attccttgga aaaatcctgc taacacaatg ctgatcggta caaggactgg
    13501 catagaagtt ccagctccta ataacagcaa acaaataatg aataagacaa atgctgctaa
    13561 aattgtccga taagtcgtaa acttccgctc aagagccggt gcaacgaaaa tcgaagcaat
    13621 agccagaagc acaccccagc caaaaaatac aaaaccgact tctaattcag aatagccaat
    13681 caataaaaat ggcgcatacg caagtaaagt aaagaatccg aaattatata acaatgcgat
    13741 tagtccaact gaacgaagtt tatgattgct taacgctttg acaccttccc aaaggcctac
    13801 ttttctcttt ggtttctcag tttcaggtaa aagtaccgct agggctataa aaccgcaaaa
    13861 catcaaaaat gctaccccaa aaaatggata acgccatgaa ccactgccca agaccccacc
    13921 gactagaggg ccaacagcca ttccgcagcc aagtgctgct tcatacatca tgattgcttt
    13981 ttctgtgtta ccaactaaaa tactgacgat cgttgctaaa gcagtagaga caaataaagc
    14041 attcccgaca ccccagccgg cacgtaaccc aatcagcatt ccgatattcc cagataatcc
    14101 gccaagacca gcgaatacaa cgatgataaa caatcctgtc atcaatgttt ttttcaaacc
    14161 gatccgacta gataagaaac ctgtaaatag catcaccaca ccagtgacta acatgtaact
    14221 agtaaataac agggtcgttt cagctggcgt agcgtttaac tgtgcagcaa tcgtttttaa
    14281 gattgggtct actagaccca ctcccataaa agcgatcata caggtagctg ctacaactag
    14341 cgctgaactt ttcccctttg tttcttcatt tgtttgttct tgtatatcta tttgattctc
    14401 caatatttcc atccctttct ctctattagc atcatacatg atttatttta catgtcaata
    14461 tgcacatgta aaacgtggaa actttactgt gatataataa agaaaataag acgagaggta
    14521 ggatttttat gaatacttta gcctatatat tattatgcgt gcttttatcc aaaccacgtt
    14581 ctgggtatga attgaagcag ctgatcacaa ttttttggga agcccatcac agtcagatct
    14641 atacaacgct tgccaagtta gaccagcagg gttatgtgac cgttttggac aaagacgagc
    14701 actctcaaaa aaagatctac cacttgactg atgaaggacg tgctcttgta gaggaatgga
    14761 tcaaagagga aactcccgct cctacgcaaa aagatgaatt tctagcaaaa atctatgctt
    14821 tttcgacgct cgataaaaca acagctactg gactgctatt cactagagaa caacagctga
    14881 acaaaatcat gcaaaaaaat caaaaaaaat tgaatgacct ctcgctagac aagaaacaag
    14941 aatttggacg ttatgttgtg atccagcgca gaattatgct ttgtcagcag gaactccaat
    15001 ggtgtcagca agtaagagaa caaatgcagc agttttttga gtaagggata aacagaacct
    15061 ttctagaaat atctattgaa gagcaaatta tcttcaataa aagagcaata cagttggaaa
    15121 agaaaaagcg atccgaacga caggccaaaa cctatcgttc ggatcactat ttttctagaa
    15181 ctgcattcaa gagactatga caaaaagcat gccaaggcct tttttcaaat aacagtgttc
    15241 catcattcat tactttgaat tgcttttagc acacttcttg aaagcgggcc aacgatgatc
    15301 aactgcaacg gtaaagcggc aatcacattc attttccaag cattcatata agcagagaat
    15361 aattgatcag gtattccttg ttccatgatc acaccgaata aagacataca tgtcaccatt
    15421 cctaaaacca tcaagcatga aatagttaag atcatttgga ccttgctttc tggatttact
    15481 ggtagtttaa aggcaatttt tttcgccagc acaccgatga tgaagacatc caaaatgaaa
    15541 gccaccacga atccaggcac tagaccaatc aacaaacttg ataaatgaag atcttgatgc
    15601 agccataaat tatacgcgct catccccaag accatcaaaa aacacatcat tgttgtaaaa
    15661 attattcctt cttttttatt tgtaggcaat ctgtaccact cctttaaatt tgtcacgaaa
    15721 atcatcgtag cacatttaaa aatttctcgt aagtactttt tttattttct gcccttttat
    15781 ttcataaact tcatcagccg tacgctcgac aaagtaagaa tcatgagaag taaacaaaat
    15841 cgttcccgga tatatttgca ttagcttctc taaagcttcg atcgttgcca tatcaataaa
    15901 attagtcggt tcatctaaca gcaacacatt acttggctta gtaaaaagca aagcaatcgt
    15961 cagacgggtc gcttctcccc cacttaatgt acataacggt ttcgtcaaag tttcctgagc
    16021 aaaccctaag tgattcagca aactacggac aagcgattct gaaaactctg tttgtctcat
    16081 gacaaaacga atgatggatt cttcagatgt catttgataa cccttttgct gatatacttg
    16141 aaagcttact ttcggagaga gaaggattcc ttctccttgt ttgcggatat gttctaataa
    16201 ggttgtctta cctgaaccat tttcaccgac aatcgcgatt tttttaccta aagaaaactg
    16261 aaaatcacca tttaccagta atgttctccc acttctttcc aattggacat tttgtcccat
    16321 gatgggatag cggctgtgga gttcaagaga ttttggtact ggaaaacgga tttgtttcat
    16381 ctgctgcggc ttggttgttt ctcctatttt ttggagtctt ctctctaatg ttttcgcatt
    16441 cttttggatg gctttttgta cactgtcttt ttgtttagag gaagacaacc gatccagtct
    16501 gatttgttgc tttttctttt tactggacac tttttccaac ttttccgctt gttttttctt
    16561 ttcttggatc gatttttcca atcgtttttt ctctttcatg atcgactctg cttctcgtag
    16621 ctgagtcttt ttctccaatt ccttttgagt gagataggca ctataattcc ccgaaaactc
    16681 tcggatttct ccgtctttta cttcccagat tttcgatgct agctcatcta gaaaatagcg
    16741 atcatgacta acaaaaacca gcgtaccata ataataacga agctcttcaa tcagtcgatc
    16801 aatacttttt ctatctagat gggttgttgg ctcgtctaga agcatccctt gttcataaga
    16861 agaaagaaga cgtatcaact cgatctttcg ttcttcccct ccgctccatt tccgctctga
    16921 agtttcttcc agtcttaact ggaaatgact ggctaattcc aaatttaggt cctttgcttc
    16981 agcgagatag gttaattgag gcaggtagtt gaaggtgatc tctgtttgga ttttcccatg
    17041 atcgggaaaa agttcaccgg ctattagttt gagcaaagtc gatttaccga caccattttc
    17101 tcctatgatc cctatacgat cttgctgata gacacgaaga gaatcaatag atagttctag
    17161 ctggttgcca aagctgactt gtagatttgt tatatttact gctaaatttt ccatatgttt
    17221 tacactccta tttcatgtaa aacaacaatt atcgatacta aaaaagagca gaaaaatctg
    17281 tttgcaaaaa aactgcatca catcttttcc tactctgaac gaaataacta tgactaaatc
    17341 accaaatctg tcatagcttt cacctattct actgtcgttc gaaaaaatgg acatactcca
    17401 tccatgaaat tgttgtttat acatgtcttt tgaatttatt tagttgttat tcaaaagttc
    17461 gaaacgtaat ttcatcgatg cagtcatttt tccgacctcc ttttttatta ggttaccaca
    17521 cacgattcaa aaaaagcaat agctactgaa tagtaactga aaacctttta ttcgaacata
    17581 gtgatctttt ctggtgctaa acgaacagac tcgtaacctt cttctaccgc ttttcctatt
    17641 gataaaataa taactggaac ataacggtct ttatccaaac caaatgcctc tgccaactga
    17701 tctgcttcga atccaccaat tggatttgtt tcatagccat gatcacgcgc aactaacatg
    17761 aattgcatag ccgctaagct agaatcgact ttgactacat cgttcatttc ttctcttgaa
    17821 aaactcttgt aataaggaat gatcgcagct aattgttgat ctctgacttc ttttggcatc
    17881 ttccctgatt cgtaagcttg gttgtagatt tcttcgccat attcgtaaca ttgcatatcg
    17941 ccaaaaatca tgaccattgc agaagaagta tcattttgtc tcgtattgaa acggatcaaa
    18001 ggacgcaaga tttcttttgc ttcctcgctt tcagcgacca caaagcgcca tggtttcata
    18061 ttgacagaag aaggtgcagt cgtcgcttct ttgatcattt ccagcatttc ttcgtgtggg
    18121 attttgtatg tttcatcata tacgcgaacg gatcttctac cagtcataat tgttgaaaaa
    18181 tcattttttt gttccattat tattaacctc tttcgtttct gttgttatat tgcaaagttg
    18241 ggtattcaat ttatctaaaa gtgttagaaa ttgtttcatt tctacatcgc tgaaagccgg
    18301 ggatagcacg gaacctgtat ctttatggtc catttcacaa tgcttgattt tcttctgggc
    18361 tttttctgtt agcgctacat acacttcccg gttgttctga ggatttctct gacgcgaaac
    18421 atagccattt gcttccaata ccttcagatg acgagtaact gctccttgat caatctgcaa
    18481 cttttcttgc aaactgcttt gtagcaatgg attttcttct ttcagtctca tcatgatctc
    18541 gtatctagta agacttatac cgatattttc ttcaaagatc tggacgattt tttgatcggc
    18601 taatttgatt tgatataaaa ggtgactaat ctcttctaaa gacataattt cctcctttct
    18661 aataattgac tcgtcaacga ttgacttgtc aataatagaa taaaaaaaga aaaaagtcaa
    18721 agaatcagct gtgtaataaa aaaaacgctc tcaatagaaa agtatagaga aaagaaattt
    18781 tttgtagaat gaacggaaaa aatgataaag aaagcacaac gctaaaacca agattattga
    18841 ttcttatcaa actatagaag atagctcttc tgcagtagat aattttacac cgccaaaaat
    18901 ttccgaaatc gccttttgca agaatcccgt cgaatattag aaattggatc agtatgctta
    18961 aacagtaata aaaaagttat gacaagatgt tgtcacaacc ttttttgagt aaacggtgtt
    19021 caaaaacttg catctgcggt aataagttca accaaacaaa aaacatgaaa atctgttttt
    19081 gtttggctgt tcttactact tgatgcaggg cagctttttc actacctctt tttaataacc
    19141 ataaaatcct attccttcat atctccagcc catcggtgtt agccaatctc tttccttagc
    19201 actcaaggta tagtgatggg taccagtttt ttcatttgga ttaaatagtc gataaattgg
    19261 cttggaacca ccagaataaa aacttagacc ttcataacgc caaccgattt ttgttaaaga
    19321 gttgatctca gattgtgcca ttgtgtaatg atggtctcca ctgtaaggat tgtataaacg
    19381 gtagacaggt tgtccgctgt ttggtgcttg ccaagcaatc ccttcatatt cccagcctac
    19441 agttggcaga taattttttt ctccggcatt caatgtatat aaatgttctc ctgtatgacg
    19501 gttatacaga cgatacatcg gagaagttga agttggtgct gtatcatact gtgtcagatt
    19561 ataggtttcg attacattgt tcaatgtaga accataatta ggcgcagtgg cataacgacc
    19621 tgttagccaa gccgtagcat ctttgtaaga gttcgtattg cttttccatg caccggaata
    19681 gtagtaaacg cctggctgaa aagaagtcgt cttcaatacc gttacgttat cttgcaatga
    19741 ttctttgtac gaaggatatt ttctaaaagc ggcattcacc gtaatccatt gcccattcac
    19801 atattccgaa gtaggcatcg taacagattg gccattatag cttcctttaa tcccaaatag
    19861 attgtaatta ggggcagaag ccaaagtact gttcccccag ccactttcaa cgatggcttg
    19921 agcaatcatg actgatgcat ataagtcatt ggcacttgcc aaactttgtg cagttgaggc
    19981 aatactatca atgaacgctt gttgagaatt agcagatgag cgagcataaa aagcatagcc
    20041 agtatagttt tcgaattcag acaaatctgc ttgatgggta tgtgtcaatg tttgcccatc
    20101 tgtcaattgg ttcgtgattt cttctgcatt ctctggattg ttctgagctt ctgctgatgt
    20161 actgtcttcg gtttgtgttt gttctgttgt ttcagttgtt ggttcttcgg ttgaaattgt
    20221 attttctgag acagtagctg actgcaaagt actttctgta tcgttttcaa ttcctgtttc
    20281 tgaagaactc gatgtctgag ttgcgacagt ttctgtcgat tcttcagcta gcactgttgt
    20341 gttggcaact gctagatttc ctaaaagaat aacgctaaat aataaattct ttgctttcat
    20401 cttttttctc ctttgaatta tgaaactttt cctaaataac agtgtaacta aaaaagtaaa
    20461 agcaagtcaa tgtccatatt taaataacat ttgtttcttt ttaataaatt tgctttttct
    20521 gcataaaaag acgtaaatga agaatattct tctccattca cgccttaaat tgatggtcgt
    20581 ttgaataact atttaacaag taattctctc tggatattat ccgttatgga ttatacttcc
    20641 tgtgtcgtcg gacggataat catgttgctg acagacatcc ggtctggcgt ttctatcgca
    20701 aatgctacag cagaagctat atcttcagat gttaatcccc attctttttg cgcttcatga
    20761 agttcaagag cagccttttt gtcgctgatg gtttgatata attcagtttg aacagctcct
    20821 ggagagatga tcgtagattt gatgttattt tgccgttgct cttgacgtaa tccttccatg
    20881 attgcacgga cagcaaattt tgttccgcaa taaacggcgg aatcaggata aacaacatgt
    20941 cccgccacag aatcagttga aatgatatgc ccagattttt gtttttccat gattggaagt
    21001 acggcggaaa ttccatttaa cacacccatg atgttgatat caagcatatt ttgccattca
    21061 tcacggtgac cttcaactaa tggagcggtt ggcatgattc ctgcattatt gtacaataca
    21121 tcgattcgac cgaacttttc catcgctaaa tcgataactg cttggacttg tgcaaaatct
    21181 cttacatctg ctgtttgaat atatagctca gcttctggta aagattcttt tattgctttt
    21241 aggcgatctt ctcggcgtgc agctataact agtttagccc ctttttctgc taatagcttc
    21301 gttgttgctt caccgattcc gcttgaagcg cccatgatga caatcacttt ttctgataaa
    21361 tcattcattc tattctcctc ctgttttgaa aaatctcttg cttatactca ctatctctta
    21421 tttcatctta ctgttcaaat aatccgttcg acaggtttga cgattttggc agggattcct
    21481 gcaacgatgg tattgtctgg gacatctttc gtaacagtgc attcgctgca acaatggcat
    21541 tctcgccaat cgttacgcct ggcagaatag ttgctcctgc tccgatccaa gcatttttct
    21601 tgatgtggat tggctttact gacaagcctc ttcgttcttt tggatcggtt agatggttca
    21661 ccgtgatgag gttgacacga ggaccaatca atactgaatc ttctaaacag atgccaccta
    21721 agtcaacgaa catcacttga cgattgataa agacattttt ccctatacta atatgtttgc
    21781 cgaaatctgt ataaaaaggc agcagaacag tcaccgtatc atctactttg tcactaatga
    21841 tattttctag ccgtttgcgg atatctgctg gttcatggta cccagtattc agcgctgcta
    21901 aaggctcttc gttttccgct tggatctgat ggatcacttc aaagagcgga tccgttactc
    21961 gaatctcttt attgacgatt tgtttttcga tgaggcttgt cattttgctc cctcctttta
    22021 tccttacttt ataacggacg gcttatgatg tctaatactt ataaaacata cttaactatt
    22081 ccttttaact atacttagtg gtatgctttt ttaaaaacag gagggaaaaa atggatattc
    22141 gtatactgac gtatttttta gcagtagcta gagaaaaaaa tatcagccga gcagcaaaat
    22201 ctctgcatat cacgcaacct actctctcaa aacaactgaa agatttagaa gaagaacttg
    22261 gtgtgacact tttcactcga ggctcccgag agatacagtt gacggattct ggtcgatatc
    22321 tttattcaaa aggaaaagaa atcttgtcgt tggtagaaaa aacggcgaac aatttaacaa
    22381 atgacgcaat catctctgga gaaatctaca ttggcggagg cgaaactaaa gcaatctctt
    22441 tcatttctga tagccttcat tcgttgatca aggcgcatcc tgatatccag tttcatttat
    22501 atagcgggaa tgcagatgat atcatagaca aactggatag cggattactt gattttggac
    22561 tcgtaatcga accgacaaac aaacaaaaat accagtcatt tcgactgcca gcccaagatc
    22621 gttggggact tttgatacat cggaatcatc ctttagcaaa gcaaacagtc atccatccgc
    22681 aggaactgaa aactgttcct ttgatcattt cccggcaaac gtcagtggac agtcaactag
    22741 ctgaatggct aggtttcagc atagatcagt tatcgatcgt cggaacatat aacttattgt
    22801 acaatgcttc tttgatggtc gataacggga cttttggtgc attagctcta gatgggatca
    22861 tcaatactta tgggacggaa aaagtattca tcccactcga tccgccatta caagcttcac
    22921 tgaatctcat ttggaaaaaa gaccagcctc tttctcctgc tgctcgtaaa tttttagatg
    22981 tcctaacgga gaaatacagt taagtagagt ttttttcctt cgctttcagc tttttaacaa
    23041 cgaataagtt aagcaaaaga tccaatgatt aaataacata agtttcttaa tttgttcctt
    23101 ccttttattt ctgataattc cagtaaatta ctataattat aaaaaaagaa ttgacaaaac
    23161 ataactgtct atataaaatt gattacgtta acaataattc gtttcatgtg actagatcat
    23221 actaggcatg aaaaatctct gaggaacatg gtttccctag gaggtttttc gtgtctattt
    23281 ttgtttggcc gcaatcaact ggtctagcaa acgtgaaaac caaatcttca aaaagggtgc
    23341 gcgcatgaaa atcaaaaaga tctttaatca aaatgcagta ttggtagatg acggtggaca
    23401 ggaaaaagta gctattggaa aagggattgg atttgacaga aaaagaaatg atttgatttt
    23461 tgatcgtgat gtcgaacgga tatttgtcat ggaaccagaa ggtcaaatca agttgcaaaa
    23521 tttacttaat cagattgatg aacgcttttt atttgcagca gaaacgatca ttgatcatgc
    23581 ggaaactgtt ttgatggaaa agttgaatga acatgtattg atcgctttga ccgaccacat
    23641 tgctttttct gcggagaaca ttaataacgg tatcgtcatc cgcaacaaac ttttaagaga
    23701 aatcgaagtg ctttacagtg atgaattcag tattgctcaa tgggcagtag agtatctgac
    23761 aaaagagctt gggattccat atacttatga cgaagcagga tatatcgcaa tccatattca
    23821 cagcggacga agcggtcgga acaataacca tcgcagtatc cgtgaagtga cgattgtatc
    23881 tgacatcatc catttagtgg aatcagaatt ggaaacagat atccattcag aaccattttc
    23941 actgaattat tcaagactgg tgaatcattt acgactgctt ctacaacgaa cccatgcgca
    24001 acaatatgct gttttagaca cggaaatcgt agaaatggtc aaacgaaaat acccagaaag
    24061 ctataagatt tctaaaaaaa tccgtgtgtt gctgaccaaa gaataccagc tcgcgatcac
    24121 gaaagaagaa ctgggttact tggccatcca tatcgagcgt ttaagaagcg caatcgtaca
    24181 aacaaatgtt aacaatcatg aggaggaaaa aaattgaaag catatctaca acgaatggga
    24241 cgttcccttc aacttccagt tgcggtctta cctgctgctg ctttactagt agggatcgga
    24301 aactggtggg catcttatag taacgacatt gtcgctcatt ttttacaagc tggcggtaac
    24361 gcagttttag gtcaattacc tttgctgttt gccgttgggt tagcactagg gatgtcagtg
    24421 gacaaatcag gtgctgctgc cttggcaggt ttggtcgcat ttgaagttcc agtaaatgtt
    24481 ttaaaaactg attcagtagc tactttattg aatatcaaac cagaatcagt tgatccatct
    24541 tttgctcaga tcagtaacgt atttattggt attattgccg gattgattgc ggcagctctt
    24601 tataatcgtt tccacgaagc aaaattaccg atggctttat ctttctttag cggcaagcgt
    24661 ctagtgccaa tcttggctgc ctttgtcatg ttgctgatca gtgcagtgct actgctcgta
    24721 tggcctccgg tttactctgc tttagtttct tttgctaaat tcattttgag tttaggctgg
    24781 ggtggtgcag gtctttatgg cttcttcaac cgtttactga tccctacagg attgcatcaa
    24841 gcaatgaact ccgtgttctg gtttgacgta gcaggaatca atgatattgg taaatttttg
    24901 gcttctgaag gaacaaaagg cgtaacagga atgtatcaag ctggattttt cccaatcatg
    24961 atgttcgggc ttccagcagg agcatatgct atttacagaa atgcacgtcc agaaaacaaa
    25021 gcaaaaacag cttccttgat gttagcagca gcttttgctt cattctttac tggtgtaaca
    25081 gaaccacttg aattctcatt tatgttcgtt gcttggccat tatatgtctt gcatgcaatt
    25141 ttcactggga tttctttagc agtgagtgca ttcttccatt ggacagcagg atttgccttt
    25201 agtgccggat ttgtcgatta cttcctatct ttgaaaaatc cgattgcgaa tcagccattg
    25261 atgttgatcg tccaaggatt agtaacagca gtgatttact actttggctt taactttgct
    25321 atcaaaaaat tccatctgat gactcctggc cgcgaggaag cagatctttc agatgacgat
    25381 actgcaacga ctaattcaga caataaatat gcggcacaag caagcaaaat ctacgctgca
    25441 ttaggcggag ccgacaatgt aacttcgatc gataactgta ctacacgcct tcgtttgcaa
    25501 gtcaaagata caggcctgat cgatcaaaat aaaatcaaag ccactggcgt acctggtatg
    25561 aagatcatcg acggaaaaaa tgcgcaagtc atcgtaggta cagaagtaca attcgttgca
    25621 gatgagatgg caaaactaca tggtggtgct gcaacacgac ctgcacaaac aaacaccgtg
    25681 gttaaaacag aaacatttgc cggtggaacg atcgaacaag atatttacgc tatcgcaaat
    25741 gggaaactga ttccaatcac tgaagtttca gatgatgtat tcgcggaaaa aatgatgggt
    25801 gacggctatg ctgtattacc agaaaatgga gaaatcttct ctccaatcgc tggtacgatc
    25861 acaaacattt tcccaacgaa acatgctgtc ggaatccaaa cagatgctgg tatcgaagtc
    25921 ttgcttcata tgggcatcaa cactgttgac ttaaaaggcg aaccattcac tttatatgta
    25981 aaagaaggtc aaaaagtcgc aagaggtcaa ttgattgctt tagttgatct tgccgctatt
    26041 caatctgcag gcaaaaacac ggatatggtc gttgtattca caaatggcga caaagtccaa
    26101 agcttagaaa tcgaacccgc acgtgatgta aaagcaaatg ataaaatcgg ctctgtttcc
    26161 aacaaagcct gagccatcgt cttccccaga cttttttatc gataaacaaa caggagctga
    26221 gacaaaaatc ttgtctcagc tcctttttcc gaataaaaac ggcgttctcc tgccaactcc
    26281 gcggtaataa gtccagccaa acgtaacata tgaagagcta ttttttattt gactcttctt
    26341 attacttgat gcagaacagc ttttttacaa cctctgataa acggtgttca aaaaccagtt
    26401 tatcggtatg aagtcaaatt tatgcaaaaa tgtggaaagc cattttcaca aaatttttct
    26461 tcatactcaa aactaatcgg ctttttcaca acctctttat acattattcc tgatccaata
    26521 tttcttttaa tgctgcttca agagaagtta atggttttcc caatactttt tctaaatcat
    26581 cagaggaaat agctaactga tcctttttaa tatcatgttg gatcgctaaa aacatgtcag
    26641 ctgcttcttg agggaatccg gcttctttta gattttcgac aaacgctgca tcacttgctt
    26701 caataatttc taacttttta cctgtcgcac gctctaatgc cttggctaat tcttcatagt
    26761 cagttagatt gccggataat tctaagattt ctggagagtc actttctact agtacttttg
    26821 ctgcagcttc tgcatattca cgtttcaatg cccaacctgt ttttccattt cctgcagcat
    26881 aaacgaattg accagttttt aaagcctgcc caatgatagg taattcattc tctacatacc
    26941 aattattccg taaaaatgta tgggaaatac ctgaatcctt aatcattttt tcagtgaatt
    27001 ggtgatctgg cgctaataca ctgatcgagt gatcggcatc tgcaaaactt gtataagcga
    27061 tataagatac cccagctgct tgtgctgcct tcactacatt cttatgttcg gcttgacgat
    27121 ttcctggaac gccagagaca aaaagcaatc gatctattcc tttcaacgct tctttcattg
    27181 agtctaaatc atcataatca ccaatacgta tattaaatcc agcttctttc agtgcttctc
    27241 ctttttcttc actacgtact agtacataga tttcattttg tggaaccatc ttttttaaga
    27301 attccagcgc ataaccacca aaacctccag ttgctcctgt tactaaataa tgcatatttg
    27361 ccactcccta ttctttttat ttgttaagat ttacagctgt acatttataa attacacctt
    27421 tatcataaag agtgtggtaa gataagtcaa gcaaaaaaaa tgaaaggatg acaactgatg
    27481 aagtattctc tgcaatttag tgatgcaatc catgtacttg cctatattga aatgtttcaa
    27541 gatacccgat cactttctag tgagatgatt gccagtagta tcgaaacaaa tgcagcaaat
    27601 gtacgtaaaa tcatgagtca gctcaaaaaa tccggtttga tctttacaaa aaccggaaaa
    27661 ccgcagcctt cccttgccaa aagtccagat cagatttctt tactagatgt ctatcgcagc
    27721 atcgaaggta atacacagct tatccaagtc gatccaaaaa caaatccaaa ttgcgtcgtt
    27781 ggtgcgaata tccaagaagc tttagaagaa agctacgctc ggctgcagaa aaaggcagaa
    27841 gaagaaatgg ctaagatcac acttgatagt ctgatacata aaatagccgt tttggaaaaa
    27901 gaaaaaagac cagaaaacac aggcctcgta gagagatttt tataattctc tctactgtgc
    27961 ctgcattttt ctgatcggaa tcgtctacat aaattctttt attctccggg ggacaaaagc
    28021 cttgtcatgc ccctttttcc tctctaatgc tcaggtcaaa gcgctgaagt catgacgtct
    28081 tgataaacgg tgttcaagta gctgaccctc ggcattagtt ctgaaaaatg caaaatatgg
    28141 aaagctattt cccatttttc ctctctaatg ctcaggtcaa agcgctactt tcacgacctc
    28201 tatattatca tggtttattg actaccggta tccagatttc tcccatatat ttcccgttgg
    28261 taactcccat ttcgactaca gcatttggac ccccaacata ggcgtaatct tctacttctc
    28321 ttaaaacatg cccaaaggcc gtattcgtca aatcatttct taattcttca ggcgattctg
    28381 ctaaagcgat tacaacaaga tacgttcctt ttgggaatgg gatgattcgg gtagtgtttg
    28441 gaacatcctg actcgtttga acaccgacat agtacatcat tttcccgtta tagggttcgt
    28501 tgactgtaaa gaaataatga ttttcagcag cttgtttcaa tgcatctatt gttccattac
    28561 cgataacttt ttgctcaaat gcttgtttct ctgcagccaa accgacaaaa tccgtattgc
    28621 tactcttcaa ttccacacca atacccgtca ccgtaaaatc tggtttttct tcgatcctat
    28681 actttgccat ttctcccacc ctttctatta tattaagttc atcttacata aaattaaagg
    28741 gaaatgacac gattacgctc ttttttctag ggatattttt attttttaac tatttttttg
    28801 ctttcactgc aatccaaatc tcactgtaaa cattactgaa atcctctccc cattttgtaa
    28861 aagagatctc gggagcttcg actaactcat aatctgaaga aggaagccat tctgcataga
    28921 ttttccccca agtatcttgt aaagtttggg gaaatggccc tttattagga aaaatggccc
    28981 aagtgtgttc ttctatagca agcatttcta aatcagcgta cggattttct tttgtcgtag
    29041 caaagccaat catgtgcatc aaactgccct tttcttccaa gcgggctcca tcaaatccat
    29101 aagaaacgtt gattacttga tttggatata aatctcctaa cttatgcatc tcttcccttt
    29161 gagtctccgt gatcgtctgc gctaactttt gtatttcttg attgactcct tcaaatttga
    29221 caggaacttt tttcgccaca cctgccacat aaaactgttc tttcttttcg atacgaaatt
    29281 ccatcgattt tcctcctctg acatcaataa aaaatgtcaa gcgtggaaaa gatttttgta
    29341 tgccgtatct gctgatttct gaaggcagat atcctgacca atcacgaaaa gctcttgaaa
    29401 aaccttcgac tgtttcatat ccatatctaa acgccgtttc tgtcactctt gatccatcta
    29461 ggagatcgcg attagccaaa gccaatctac ggtttttgat atagtccatt agcgacatcc
    29521 ctgcaataaa cgtaaagatg cgttttaatt gatattcaga gattcctgta ttttcggcta
    29581 ttattttata ggaaagtcct tctgtcaaat gttcttcgat gtaatcgatc aattggttca
    29641 gatctttaag catatttatc ttcctccttg cttgcctttt tatcatatta tcaaagaaag
    29701 tcaattactt gatgaatcag gaaccaattt agtcagatgt agaaaatgtt gtatggatct
    29761 aaagaaaatt ctcaagcagc gtttcgtaac ggataattgg tattatctgc ctatctatgt
    29821 tttaccttta gaaaaataaa ttttggggaa gacacattct aagtaaacaa agcggtaaaa
    29881 atatctttta ttcccttagt taatccgctt actacctact gtacaaagca aattgttgtt
    29941 caaactgctg atcagaacgc tgcttggatt tttctttgcg atacatcata cctcttttat
    30001 caattggaac aattatttat ttctaacaaa atcacttcat ttttgtcaga taaataaaaa
    30061 acaaaaaaag ttccatcttt aaaacacgct attcttttac tttttctcac attctctaag
    30121 aaaagaaaag gagaatgaat ttttgacaat tcgagcaaaa gtgattatca aatatgtaaa
    30181 atcagctact tttttcttaa acgtttcttt atactaaata taaataagtg tttcatgaaa
    30241 ggagaactag atgaataatt ggcttgagta ttttcctgaa aacgttttgg aacgcggtta
    30301 cagctatcat ctgcacggtt ttgtccgtca tttgaactat acaagcaagt acttgtccgc
    30361 aacagtttct ggcacggaag attataaggt agtgatcact tgggatgaaa agaccaacat
    30421 gacttgtgat tgtctatatg ccatagaagg aaaaaaatgc aaacacatgg ctgctgtctt
    30481 attcgcttac gaagaacgtc caataaaaaa atccaactac tcactttccg aactctcgtc
    30541 ccttgtttcc tccgctagtt cctctcttgt acgggagctt ttaacagaga ttcttataga
    30601 acatccgcaa tttatcgaac gcttcaaagt taagatgcca tttcatgcaa taaattattc
    30661 agataaatta acaactatca tccataaata cgatcatatt atcaagaaaa acaaaaacag
    30721 gaaaacagcg aagttcatca tggagatgcg aaagttcatc caagaagcag ttgaatcgct
    30781 cattcagcaa aatgcctatc ttcccgcatt cgaattgatc aatgaagtga ttgctactct
    30841 cgaaacattc tattgggagc ctgaggatga acgaaccctt cttctgatag aggactgtta
    30901 ttatttatgg aaagaattat tagcagaagc acctcatgca gaaaaaaggc aaatgttctc
    30961 ttggttcgtc tgccaagtcg atcatactga cgcctcttat tcaaaacgct actccataaa
    31021 aatcctgaaa gaagactttc gggaaaaaga attttctaac caaaagaaaa aaatagataa
    31081 aaaaactaaa gaaacggtaa agaaagacga tttcaatgaa aaatagcaag taaacgataa
    31141 caaccatcct aaatccacaa gaaatacagc agaccttgct ttaaaaacac gaatcatgca
    31201 aaggatgaag taaagaagac ttaacgcgta cccaatatta gtcatttgac tattaggtac
    31261 gcgttttgtt ttataagtca tgacaaattt ccttcgttgc tgggtagatt ttccggcatt
    31321 gttgatagat tttatgatac ttttctacat tttgtaagag aggttccact gcatctgtat
    31381 attggataaa attctgacaa caactttcag gtgtgtcgaa ccactctaat ctttaaagat
    31441 aaatattaaa ataacgatta ataatattaa cagtctttat ttttcaccac caaaaaccta
    31501 tctttgcata tactaaatag acgtatatac acagataggt ttgtcttgtg aaaaataatg
    31561 attatcctta ccaaaaaatg tttgtttttc tacatgttcg tacttatcaa aaaatatgat
    31621 ccttattgaa attactggct atttatattc caacgacttc ttgtgttaat ttttgttatc
    31681 tcatagtaaa ttatgtttca ttttcactta atttagttgt cacgtgttcg tcaattcgta
    31741 aaaacggtaa atatataaac acacttgcgg ctataattaa tatttgtaaa acagctgctc
    31801 gccagtctcc ggctgttgct aaaaatcccg atataactgg tggcgtagtc caaggaattt
    31861 gcactactac atgattaatt aatcccacag cagttgcaaa atatgctact gtcaaagaaa
    31921 aaatcggtgc aagcacaaag ggaataatca gactgatatt aaatacaata ggcaatccaa
    31981 aaataatagg ttcgcttata ttgaatatag cgggagttac cgataattta gcaaagtcct
    32041 tataatcttt tctacgactg aagataaata ttgcaatcaa taaagcaatc gtatttcccg
    32101 atccacccat taccgcaaag gtatctttaa aggccatatt gataatatgt ggaatttgct
    32161 ctttgtttgc ataagctgcc atattttctt gcatattctg aatcaaaaac ggatctaaca
    32221 atgaacctga tattactgct tgatggatac caaatccaaa aaatagatta gcaatagatg
    32281 taattaacaa aaatcctggt aaaccagttg taattttact taatggctct gtaactatcg
    32341 tggtaattag cttatttata tccatattaa acaattgaat gattataaat gacaataaag
    32401 caaacgaggt aatcgtaata atgattggga ttaatacatt aaaagacttt aaaacagcgg
    32461 gaggaatatt tccggatagc tgaatctgta gtttcttatt attagaaagt ttaataaata
    32521 attctgtagc cgctaatcca acaaatatac ctacaaacat tcctgaagct cctgtatagg
    32581 atataggtat cacacccgtc acttcaacag ctttatccgc attttcagga gtaaacatca
    32641 tctgcatagg tgttaataca acaaatgtcg aaatagcaac taataccgct gcaatatgat
    32701 ttgtataatt tctgcttgca cacatatgat aagatactgc tgctacaact aataatgtag
    32761 ttatgcttaa tgtaccattg gcaattgaag taccaatttg ttgtaacgtg gtcaatgtac
    32821 tagtatcgac aatttccccc atgaatcctg ttggttctaa gatcactgaa ttaataagtg
    32881 tcacaaatcc tgctaacatc ataaatggca taaatgtagc aaacgcatct cgcaaagttc
    32941 ttaaatggat ctgattcccc aacttagtcg caactatacc aaatttttca aatacaatgt
    33001 ccttcctgct acttttgccc atattttttc tcctttatgc ttattgtaat cgattacttt
    33061 ttccacccaa ataatacatc acttatcaaa aaaagtaaat acttttaatt aaatatatat
    33121 atctttttta gtataaaaaa ataagctctg aaaaaataat tttcccgagc ttatttttca
    33181 acaaaaacag cttcaaaaac aaagtcttga tataaatgtc tcgataccga atactcgaaa
    33241 ggtttaccag acgatagagc aacaactttg tctatctcaa tcataaattc acctggatct
    33301 atttttaata tttttgcgtc aaattcattc gcagtatctc ctttgatacg tatgtgcgaa
    33361 gtctttattt ccagacccag ttcatttgtg atataagcat atacagattt ctttaaatgt
    33421 tttggttcca agcctggtat gactgctaat ggcatgaatg tttgctcaat cgagtatggt
    33481 tgacaatcaa taaaacggct tcgtacaata ttatatacat agtcttttac actgatactt
    33541 aatttatcag ctactttttt cgatggaagt tcaattgaga aagtatggat ttctgaatct
    33601 acgtgttctt caccaaccac gctaaacaaa ccttcgtttg gaccaagaac tcgtgagtta
    33661 tcaattggtg ttctgacgac ttcagtccca tacccacttc tacttcgcaa aactccttcg
    33721 gaaacaagta agtctacgcc tttctttact gttaacttgc ttgtatgata aataactgcc
    33781 aattcatctc ctgtaggaag tttctcacca attttatact ctccatttac aatttttttc
    33841 tttatatcta agtaaatttc catatacttc gcaatttttt tcaatggtga ccctccaatc
    33901 aaaaacatat cttcatctat actttaacat aaataaaccg ataaaatcca catcaaaatt
    33961 atacacacaa taaaaaagat atatatattt aaacaaaact atttacttta agttgtaagc
    34021 ggtgttatca ttaagtggaa acaggaggta aaaaaattat gacaactatg aaaaagctaa
    34081 cttttccaga tgatttttgg tggggttccg cttggtcagc tgagcaagca gaaggaaaag
    34141 gagaaacagg aaaagcagag actatctggg atcgctggtt taaagaacaa ccttaccggt
    34201 tttatcaacg aatcgattcc tctattacaa ccgatcacat ccatcaatat aaagaagata
    34261 tagaactaat gaaaaaaact gggcataact cctttcgcgt ttctatttct tgggcacgaa
    34321 tgtttccaaa tggcggtacc ggaaaagtaa atcccaaagc catttctttc tatcgaaaat
    34381 tattttccga aatgaataat aacggaatca aagtatttgc caacttatat cattttgaca
    34441 tgcctgctac tcttcaagat aaaggaggat gggaatcacg tgaagtagtt gatgcttatg
    34501 ttcattacgc tgaaacttgt tttaaagaat ttggtgactt agtttgtcat tggtttacct
    34561 tcaatgaacc acttgggcct attttaggta cttatcttga agattttcat tatccaaacc
    34621 tcattgactt taaacgcgga gcgcaagcag cattcttcac tatacttgca cacgcaaaag
    34681 caatacaagt atttaaagaa ctagctcttc ctagtaaaat aggcgttatt ctaaatttga
    34741 gtcctacttt tcctcgtagt caaaatcctg ctgatgtgaa agcagcagaa atcgctgatt
    34801 tattttatac gcggagcttt ttagatccta tggttaaagg gaatttttct aaaaagctta
    34861 tcctgttatt acaagaatat aatcagcttc ctgaagatta tacgaaagaa gatcttgtat
    34921 gtatctacga aaataccgct caaattcttg gactaaacta ttacgagcca cgtcgcgtca
    34981 aatctcgttc ttctatggtg gatacatcta gtccattttt accagagtgg tttttcgaac
    35041 cacatatcat gcctggacga cgaatgaacg aataccgggg atgggaaatt tacgaaaaag
    35101 gaatttatga tttgtgcatg gatatcaaga accattatgg aaacatcgaa gcatttatct
    35161 cggagaacgg aatgggcgtt gcggatgaag aacgtttcat ggatgataca gggcaaataa
    35221 ttgatgatta tcggattgag tatatcaaag atcatcttgc ttacttatgg aaggcaataa
    35281 atgaaggatg taacattaag gggtatcatc tttggacatt tattgattgc tggtcatgga
    35341 tcaacgccta taaaaaccga tatgggctgg tttcactcga ccttactact caaaaaagaa
    35401 caattaaaaa aagtggcgaa ttttacaagc aattaagtga tacaaatgga tttagatacg
    35461 atgtagactt gttagttgaa taatattcta aaattccact tgaagtgaac acattagaaa
    35521 acaagaataa tgagaaatac caataacaaa tactttttct tcatacttca tttatacttg
    35581 tcagtataag agactgtgac aaatgtagtg atcctcagaa gttaggtctg gaaatctaac
    35641 ttgagaagat ttttttaaac tacgtccagt cttttttatt tgtttaaaat tgaagttgac
    35701 aagtcttcat taaaaattta gaatattcac aagtttgacg acaattatga aaatttatgt
    35761 cgtgctaaag gagttttgtt attggatgaa aagttagata taaaaactgc gatcaaagac
    35821 acgttaccta ccgtttttgg ttatatcggt attggacttg catttggtat cgttgggaaa
    35881 gctgccgggt ttcatccgct ggtcgtcacg ttgatgtccc tgctgatcta tgctggttct
    35941 gcccaattta tcacggtcag catgcttgct agccatagtc cactgctctc catcgttctc
    36001 tcgacctttc tagtcaattc ccgaatgatt ctgatgagta tgacgattgc tccttatttc
    36061 aagaaaaatc ggctgcttca aaatctgctg attggcacat tgctgacaga tgaaagtttc
    36121 gcactgggaa tgaataaatt gaactatact ggacaaaagt taaactttcg ttggatgaat
    36181 acagctaatt ggatttctta tttgacgtgg gtgggttctt ctcttgttgg cgcactttta
    36241 gggaatttta ttactgatcc gaaaaagttt ggtttggatt tcgccatcgt cgctatgttt
    36301 atcgggttac tttatcttca agtcatttct gatagaaaca cctctaaacg attacagttg
    36361 atcctgattg gtctcacttt gattctggta tatgtcggac tgatttttat cccaagtaac
    36421 ttagtgattt tagttgtaac actgattggg tgcggattgg gggtgtggat caaacatgcc
    36481 ttcttttgag tttacattgc tgactatttt aggttgtact ttagcaactt ggctttctag
    36541 gatcctacca tttgtcttac tgaaaaaatt cgatttgccg caaccgttgc ttgagtattt
    36601 aagttttgtc ccgatcgtca ttatgtctgc tttatggttc agcagtctat ttacccaaaa
    36661 tatcggacat ctgccgcaga tcaatctcga aaatgctttg gcttcagttc ctacactttt
    36721 agctgctatt ctttcaaaaa gcttattggt gatcgtcttg gtaggaattc tttccctttc
    36781 gttgattcgc ttattataaa taaaaaaaca agagcttatg gcaaattact gtcatgcgct
    36841 cttgttttta tcaaagtcaa ataagaaaaa aatcggcgta ttcttgctta tccgttgaac
    36901 ggaccactaa caacgacttt tcctaatata tgtcctgttt ctacgtcttt ggttgcttgt
    36961 ttcaagttct tcgcattcat cccatctgaa tagaccttgc tcaatgtact gcggataatc
    37021 ccttcatttg ctaaagcagc aatgtgcgct aaagcttctc cttgcgtttc gatttcatag
    37081 ttatagtctg ttttagcgaa catatactcc cagtcgaagc tgatagattg gtttttccaa
    37141 acacctatat ctaacggctc tttgatccct acgatcgttc ccacatgtcc aaatggtcgg
    37201 acaaggtgtt tgatttgatc aaagtagcgg gtaatatcga ataatacagc gacatactct
    37261 acttgatcga ttcctaattc acgaaaactt ttacttaagt cgtgatgata atcgattgga
    37321 tagtctacac ctgttttttt cagccattca aaattcttcg gacttgcagt cgcgtagact
    37381 tctaggccag cccaatgcgc tagttgattc aaaatcgaac cgacgccccc agctccgttg
    37441 atcactaaga tttttttccc gcggttggca ttttcttctg gagtcagacc aaatttttca
    37501 aataacaatt cataagcggt caatgaggtc aacggcaagg ctgcggcttc ttcatctgaa
    37561 agattttttg gtgcaagagc gacaatacgt tcgtcaacca gttggtattc ttgattgctt
    37621 cctgctcttg tcgttgtacc tgcgtaaaaa acgcgatcgc cgacagaaaa tttttgcact
    37681 tcatcaccta cagcaacaac tttcccgact ccatcaaacc ctaagatacg taaagcattt
    37741 cttatcccgt tatcttggcg aagtttcgta tcgacaggat taacagatac tgcgttgact
    37801 tttaccagaa gatcccgtga actgggtaca ggtatctgtt ctttttcatc aataaagctt
    37861 tttggatcat cgatcgacaa cccttcataa aaaccgacta ccttcattaa ttccttttct
    37921 gacatacgaa ttcctcctta gcttcatcaa gttggagcat ccatacagtt ttcatttcaa
    37981 tatatatagt atgctatttg ttccactttt attcatcata attattctct tacttaaagt
    38041 aaactatttg cacttttttg gttaaaaaag aggttgcgac aagacttttg tcacaacctc
    38101 ttttaaacgg tgttcaatca actgaccctc ggtattcgct caaaaaacga caaaatatga
    38161 ggagctattt ttgtttggct gttcttatta cttgatgcag gacagctttt tcacgacctc
    38221 taattcacgg tgttcaagca agaaatcatt gttcactgaa aagcgttgga tgttcttttt
    38281 caaaggtgtc taagatctct cgtacttcat cagtagattt cgtattcatc aactgaactc
    38341 gcaagtcatt tgctcctgga aatcctttga tatagatttt aaagaaacga tgtagtcctg
    38401 tgatggaacg cgggatgatt tctgaataat gatcttgcag atctagttgc agacgaagta
    38461 gtccgatcag ttctttttct gtatgttccc tcggttcttt ttcaaatgca taaggattct
    38521 tgaaaatccc ccggccaatc atgatgccat ctacgccata tttttcggcc aattcttctc
    38581 ctgtctggcg atctgggata tctccgttga tcgtgatcag tgtttgcgga gcgatttggt
    38641 cacgtaaagc aatgatttta gggatgactt cccaatgtgc atctacttta ctcatctctt
    38701 tacgcgtacg caaatggaca gaaagattgg ctatatcttg atgaagtagg tgactgatcc
    38761 attcttccat ctcagacatt tccgtatagc cgatacgcgt cttgacgctg acaggcaaac
    38821 cgccagcttt agccgcttca atcaattgtg ccgctacttc tgggcgaagg atcaagccac
    38881 tgccttttcc acgttctgct acattaggga ctgggcagcc catgttgata tcgattcctt
    38941 ggaagcccat ttctgccata gcaatgctca tttctctaaa aaattctggc ttgtctcccc
    39001 aaatatgggc aaccatcggc tgttcatctt ctgtaaaggt caatcttcct cgtacactgt
    39061 cttttccatc tggatgacag aaactgtctg agtttgtaaa ctcagtaaag aagacatccg
    39121 gtgctgctgc atgctttacg acatggcgaa atacaacatc tgttacatct tccattggtg
    39181 ctaaaacaaa aaaaggcttt ggcagctctt tccaaaaatt agtagtcatt tatttgttac
    39241 tctccattct atcaagcagt gaagtcgcaa tctttctcaa aaacattgtc attatactag
    39301 gaaacaaaaa aagaagcaaa gtgaacagct taaaagatgt ttctgattga gcaaaaaaag
    39361 actgtgacaa aaatcttatc acaattcctt ttccggaata aatggtgttc aaaagcttgc
    39421 atctgcggta ataagtccaa ccaaacgaaa aataggagga gctatttttg tttggctgtt
    39481 cttattactt gatgcagaac agctttttca caacctctcc actaaacggt gttcaatcag
    39541 ctgaccctcg gtattagctc aacaaacgac aaaatatgag gaactatttt cccgtttgtt
    39601 tctctaatac tcaggtcaaa acgctgattt cacaacctct tttctaactt ctcaatcctc
    39661 ttcctttatt gatcaaatac caagtaacgg cgtacaaaac aacgatgaat agcacaagta
    39721 cgatcatcga tgttgctact ggcacatcga ttgttccaag gaatccgtaa cggaaaccga
    39781 agatcatata aacgatcgga ttgactttcg aaacagcttg ccaaaatggc ggcaacatag
    39841 agatggcata gaaaacgcca cctagataag ttaacggctg caagacaaat gtaggcacga
    39901 tcgatacatc gtcataagac tgagcaaaaa tcccgttgag cagtcctgct aacgaaaaca
    39961 agattgccgt catcaataat gtaatgatga ccatgaacca tgaatacaca tgaagtggta
    40021 caaagaacaa ggaaatgatc gttacgagag agccgacgag aatactgcga cccactccgc
    40081 caatcacaaa gccccaaatg atcacatgcg taggaacagg cgctaccaag atttcttcga
    40141 tgtttttctg gaatttttga gagaagaagg acgaagaaac gttggcatac gaactagtga
    40201 tagcagacat cataatcaaa ccaggcacga tgaattccat atatgaaaaa ccacccatgt
    40261 caccgatccg accaccgatc atttttccga aaataacaaa gtacaaggat gtcgtgatca
    40321 caggcggaac cagtgtttgg acccagatac gcaaatagcg gttcgtttcc ttgatagcta
    40381 aactttttaa tgcagtaaaa taaagattaa acatttttct cctccgtatg attattttct
    40441 tctgtgattt tcaaaaacag ttcttctaaa cgattcgact tattcctcat agaaagaact
    40501 ttgattcctt gtcttgtcag ctgatcgaag attccgttca ccccttgatt tcgttcaact
    40561 tcgacagcga ttgtctgttc atcttcaaat gtacaagcgt atccttcaat cacaggtttg
    40621 gtctcatatg gcgctaaatc aaagataaat gtttcaaatt gcagtttgga tagaagctct
    40681 ttcatactag tattctcgat cagttcacct gattggatga tcccgatgtt gcgacaaagc
    40741 atttcagctt cttctaaata atgcgtcgtc aaaatgatcg ttgtaccatt ttcgttcaat
    40801 tctcggagaa actcccacat ctcgcgtctc aattcaatat cgactcctgc tgttggttca
    40861 tccaagatca gcaaacgtgg ttcatgcatc aatgccctag caatcatcag tcttcgcttc
    40921 attcccccgg aaagcatacg tgcacggaca tttctctttt cccaaagatt ggactgcttc
    40981 aaatattttt cgctgcgttt catcgcttct ttgcgagaga caccgtagta gcctgcttga
    41041 ttcacgacga tttgctggac cgtttcaaag ggattgaaat tgaactcttg cgggactaat
    41101 ccaatctgtt gctttgctcg gactagatcc gtatccagat cataaccgaa gacattcact
    41161 tttccagatg ttttattgac taaagaagta atgattccga tcgttgtaga cttcccagct
    41221 ccatttggtc ctaatagcgc atagaaatct ccttctttca cggaaagatc gattcctcgc
    41281 aatgcttcga ccccacttcc ataaaccttt ttcaaattat ttatttcaag cgcatttgcc
    41341 atttatttcc tatctcctta tagacatgat tctttattgt atcatggaat acagcagatg
    41401 aaaaaacaac ttggttccag cgaaaaatca ggttgctggg ctcaggaaaa tctcctaatg
    41461 aaagacatca atcggacgaa gtcagacaac cttttcagaa aaaacaaatc gaagataaag
    41521 gattactatt catatgtgca acaaaaggag cgtgagacaa aagtaaagaa cacttttgtc
    41581 tcacgctcta aacacgtata aacggcggaa gcaaaagcca agatttttga tccaataaat
    41641 actttcgatc acttcgccta tcccaataca tgccactgcc gcttttcatc aaataacgcg
    41701 gcttccgttt ttgcggtttc tactaaagtc tctgggtaat ccaaaacttt cagcaaggca
    41761 atcgcatttc gggtccttga aggtccttct tttaatttga aatcaaaact gattcctttt
    41821 ccttctgtta cttgttcttc aaagtggaca ttctcacaat aattctttaa aatctctgtc
    41881 aattcgatat catgtgtggc gacaaatgct aaagatggat actccgcaag ccaccgaacg
    41941 actgaagcgg aagaagcaat ccgttcgatc gtatttgtcc cttttaaaat ctcatctacg
    42001 aagcagtagc agcgttcttt ggaagccact tggtccaaca aacgtttgat cgatttgatt
    42061 tctgaaacaa aataactgtc gccttcaaat aaatcatctt cgattgccat ggaagtcaac
    42121 acatgtccag gttgcagggt aaactgttct gcaatcgctg tttggatcgt ctgtgctaga
    42181 atacagctga tggctatact tttgacatac gtagattttc ctgatgcatt ggaacccgtg
    42241 actaatgtat tcttctgcca gttgaccgga ttcactaccg cttcttttaa caatggatga
    42301 tagatatctt tcgctgtcac accgccctca gtaaactctg gtcggcaagt gatgggcata
    42361 taagtacggt aattcaatat tgcagcggct acttctaatt ctcccagcaa ttcccacagc
    42421 ttgatggctt cagttgaatg cttttctaat ttagtgagaa cgaaatgata ggagataaat
    42481 ggcagcatga acatgatgtt gacatattca aaaatcaact ctgcttcact cccactcttc
    42541 atacgaaatg aaaaacctaa tttagggatg tctcttaatg gtttagtttg ctgtttcagc
    42601 tcttcttgaa gtggtgtcgg cagttttgct agctgattgg cggctgaaat cgttgaaacc
    42661 aaataactca tgctattcag ctctgtttct agtgtttgtt tcttcactag ataatagacc
    42721 gtattgaata ctaacccacc gattgccaaa tagattccta gtacttgtcc taaaagaagc
    42781 aaacccaaac ccaccagcgg aagcaagcca agaaaaataa ataaacctac gcgtccaatc
    42841 tttttacttt ttccatccgc tagatattgt tttgcaaaat tatgatcttt ttttcctaaa
    42901 cgagcgaatt gatattggat tttttctctc agttgaggat tttcctgata aaaagcaatc
    42961 agtttttcta actgctgatc ttcaccaaaa tcaaagctgc gtaaacgttg atacaacgct
    43021 tctgaaccta cactagaata ggtactgttg attccttcga atacttcgaa catatccagg
    43081 tcataccaag taagatcatc gatttcgcta tcccatgaac ggaacttctt ctccgtgagc
    43141 catgcttcct tcaagctttc ttctttatca aatctcggtt gataaggaat tttttcccat
    43201 ttgctacgaa ctgtttcctt taatttcaaa cgtgaaaaaa tgtctaaagc aactaataaa
    43261 ataacagctg caataataat taaaacgata aattctcttt gacccatatc cctctccgtt
    43321 cctttatttg ttagctttag tatagcactg ttgcgctatt tgaaaagagg gtaaaaaaac
    43381 acctttcaaa ataaaaggtg tttttttctt gtatattaca tatttatctc tcatttatca
    43441 aaattgacaa ataatcatag ggaggaattg ttgttaattc ttgtttattc tccagtgcat
    43501 tgacaactgt cccaagggaa aggatagcct caggtactat tttttcttgt tctgttggat
    43561 tagctaagcg aagaacaaat ccttgttcat gaaaataaga aggataaatc gatgatacaa
    43621 ttaagtcttc aggtaattca atcaatgaaa atttacgagg aacatcaagt tcattttgta
    43681 atggccaaat cttgttatct aacctgtgaa taaatacatt cagtttctgc ttttgataag
    43741 aaatagattg ttctaaccgt ttttctgcct gttttgccac tttgaactca ttaaatcttc
    43801 cttcttctac attaaatcca aaagtgactt tatactcacc tatctcttga gctagcggtg
    43861 tttccatcat gacatgccct tcatttgtcg tatctcctga agctcgccca ggtcgccaag
    43921 caagattagg ttttccaagt tcccctgttg ttgaaaataa ggttatgatt aattgatttt
    43981 ctatacgttc atactctttt aaaccatctg cataaaacgt aaaataatga tcattttctg
    44041 ccacagagac actcttatcg aatatttcta aattaacagg tttctctacc atagtttctg
    44101 accaattcgg aggaatgaca tcaggggtat tttcaataaa tccgttttga atttgtgcaa
    44161 ttgatttttc agatttatct aaaacattaa ttgctaagcg catacgatgc gagtaaacag
    44221 tattatgaat tttgagtgtg ccttcaacga tttgactttc tcccagactg aacgatattt
    44281 cataagatat ttcttccatt tcgggttttt cagataaacg atcttttaaa tctttaggaa
    44341 gttgagcttt tccgtaaaca actaatgttt cttgtaaaga atctttatat actttagctg
    44401 tttctaattt tagagatagt tctgtatctc cttctaatgg tgaataatca tacgtatcgc
    44461 catcatttgc tgaatcgatc aaatgaacga aatcaagata ttgtctatct ccaactatca
    44521 aattcaattg cccatcttta aaagaaagtt tatatttatt gttacttatc tcagttttgt
    44581 ctatctcgat tgctgtttct aatttttcat tatttttctc aaattcaaca acagtgtatc
    44641 ctagtgctgg cacatttaca tttattttta tgtcaagttc ataataagct ggttcatcaa
    44701 aatattcgaa acctgtagca atcatccgat aagcattttt ccgttctgga aaataaactt
    44761 ctttttcaat gatattatag ggactacctt tgaagtgtat tttctttgaa tcagctacta
    44821 catgcacgat tttcgatcca ctataattct cagtcgttgt gttgaaaaca aggacttcat
    44881 tcgattttag ctttaatgca tcagccatcc gttttacaat aaggttttcg attccgtcag
    44941 ctatctcatt agcttctttg attcgatgga aaatatcttc cgcaacatta tctgaaacac
    45001 agccaccaat actatcatga gcctggtttt gcaaaatctt tttccataac cgacgaagta
    45061 ggcctttact gatatggata ccatttcttt ctgcaatcag catgagcggc tcaactcgtt
    45121 ttagaatttt ttgttccaat ttaaaatttt ctattttcat ttttgttctt actgagccaa
    45181 tagaacgatg aacacgagag taaaccggct gacgaagttc acctttatat tcaggtaatt
    45241 cttttttgtt tctgataact tctacataag aaggataatc tgaaataata ttgtggaatt
    45301 tactgtattt attcaacttc tctacgactt gatcaaaatt taaaatcatt gacttttgat
    45361 ctacacccga aggtactaaa atatctttgt tttttccttg catatgaagc atttcaactg
    45421 cttcatccaa cttttctttt gcaaattcag gaatatcttt aattgcgtct aaggtcatca
    45481 ttccagtcat ataaccaaat ggaaaattca tggcataaac aaatttatct cccaatcctt
    45541 tccagataaa ataaggagat ggaaccatcg tttcaaagtt taatcctcgc caacacatga
    45601 aattatcgat ttctacttga tgcaaaagtg taggcaaatt cgcattgaac ccaaacgtgt
    45661 caggcaaata ccctaccatc attggatctc catatttaag gatcgtatca ctgattccaa
    45721 tcattaagtt tcttaaaatt gattcagcat ccactaataa ggcatctgtt tgtgcatacc
    45781 acggtcccac gaatagttgt cctgttttca tcagtcgttg gataacctta agcttttgcg
    45841 gattgatctc caaatactca tcgacgattg aagtttgtcc atctaaacaa aagtttactt
    45901 tagggttcct ttccaattca tctagaactt cagtaaagac ctgatcagac aatacgatgg
    45961 catcttcatt tgtaaaatac cattctctgt cccagtgcgt gtgttgaatt atatgtgcat
    46021 ttaccatatc agattctcct actttccttc ttctactgaa tttacttgtt gttctttttt
    46081 atacttgatg gattttagtg cattgaccat taacgctgta accactcccc caataacaat
    46141 tgatactaaa tataaaggta tattcgtaac aaaaggaatt gccataaagg ttgaaagcat
    46201 tgtttcttga gtaacaccaa aaaatccatt tagaccacat gctactgcag tgcccaccac
    46261 gatgcttggt attactcgta aaggatcttt cacggcaaac gggatcgtcc cttcagtaat
    46321 ccccacaagg cccataatca tagctgtctt tgcattatct cgttccgatt catcataaag
    46381 ttttttgttt aaaattgttg ctaaaaacat tccaaatggc ggaatagcaa ttcctaataa
    46441 catggctgta ctaggtccaa agcttccgct tgaaaaagaa gcaaccccaa aagcatatgc
    46501 gattttattg ataggtcctc ccatatcaaa cgccatcatt cctcctaaaa tagccgacat
    46561 caaagcagca tttgctcctg acatgccttc tagccaatta gtcagtcctg ttgtcattgc
    46621 ttgaataggc gtagctagta cataccataa tatgattcca acaactaatg tagtaacaaa
    46681 aggaatcagc ataattgatt ttagactaat aacttgcatc ggtactttta cttttttcaa
    46741 tgcattagct aaatagccac aagctaatcc agcaaccatt cctccaataa atcccatacc
    46801 aagattttga cacatcatcc cagtgataaa agctggtgct agaccgggac ggtcagcaat
    46861 tgaatatgcg atataggctg aaatcatcgg tacgataaac tgtaatcccg tctgaccaat
    46921 cattcgcagg actccccaga atgtatccgt ctggtcccaa acacctgtgc caccaaaaat
    46981 gactgcaata gaagttagta accctgctgc tacgacaagc gggatcatat aggaaacccc
    47041 actttggaaa tgatttagaa tttctcttcc ttgtttcttg aattccatta gtaactcccc
    47101 cacgatatat tttttatttg ttaattaatg caattgcttc atcaatcgtc tcttcaccat
    47161 cttttactac ctctgaggtc cctactgtta aaatcggttt accgtcaaag cgacctcgtt
    47221 ctgaaacatt tgtatctact gcaaatatga caacatctgc ctcggaaaca tccgaaattg
    47281 aaagtttgtt ttctatcccc atagccccct gtgtttctac tttaatatct aaccctttct
    47341 tcttcgcaaa cttttctaaa ttagcttttg ccatataagt atgggcaatc cctgcgatac
    47401 atgctgttac tgctactatt tttgtcatat gaaattcctc cttttaaaat gatagagctt
    47461 ttaccaattc ttttttattt ctcgaatctt ttattctttt aacaaactcg tcatccatca
    47521 atcttccaga taattctgct aaaatacgta aatgattagt acctttatca gtgtctttta
    47581 ttgctaacaa aaagacgaga tcaactggtt gatcatctaa tgcgccccat tcaacagacc
    47641 gctttaactt ggctacagcc actgttgatt caattactgc taggcttttg ccatgaggaa
    47701 tggcgatatt tttcccaatt cctgtggtca tatggttttc tctgtcaaat acgtcttttt
    47761 gaaattgttt gtggtcagac acaaacccat tagtctctaa caatttcgat aatttttcga
    47821 ctacactttt tttatcagat gcatttaaat ctatcactat attatttata ttgatttttt
    47881 cactgatcat ttataagcct tctttcaata tataaactaa tttttcaaac gacgaactcg
    47941 tttttaaatt tgctaataac tcatgattcg aagcgatttc attgaatgtt tcataaattt
    48001 tattcaattc aggttttact ttttcattaa gtgcaaaaaa gaaaacagaa tgaacaagtg
    48061 tcccatccca atcaattcct tctggattaa cgtagacaca gattactggc ttttttataa
    48121 aatctacttc gatatgcggc atagcaaaat cattcatcgc tgttgaagat agtttttctc
    48181 ttttgattgc tccctctagt atcccttctt tcgcgtaatt ttcttttacc aattttttgg
    48241 taattatatt taaaacgttc acttgattat cctttatttg gtgggtaaaa aagcaaaatc
    48301 gtttatctaa taaatttaga aaagcatctt ctgtttgttc atttaactgt agatactgtt
    48361 ttacttttct gacatctgcc cctaacatca aaggactaac aacaataaca ggaaaatttg
    48421 tattttcaat cggaatcgtg cttataacaa gttcatcagt gacttttcta tcttgtagtt
    48481 cattgatgct tagtacatct tctatctcaa ccatatctgc aaaaccgtta tttattcttt
    48541 gctctaataa ttttgatgtt ccatatccgc tactacaaac taatatcact ttcgttttgt
    48601 caggtttgta acgatccaaa agtgattgaa tatggagtgt tacgtaagcg atttcatctt
    48661 cattaaaatg tatacagaat ctctcttcga tttcctctaa taaatctaca cttatcataa
    48721 atgacctttt aaaagaatat ttgattttgt ccgtataagg gttatagata tttactccca
    48781 atttgagtct tttaactgct gcattgagat gtaatactag attattgatc aattcttcat
    48841 caggataaaa ttcatttaat ttaattctta ctaactcttg tagctcaaca cccatatcta
    48901 aataggaaag cttcgtttga tctaagtcgt gattcatagc atttttttca atcgaagtaa
    48961 tgtgaatgtc aacataagct tgttcatcta ttggcacaat aattgaaaat gctttttcaa
    49021 tcattttgat taaaataagt gtattctttg agatttcttc ttcagaatta ttactacgtg
    49081 ataagaaatt gcttgtttca atcctttcta tcgcaatagc aaggtggata gctaaagttt
    49141 gaaattcata atctgtaaat ttcaattgtg tcttattcat gtatcgatta agcaattcgg
    49201 ctactttttt caatatgtcc ttatttaaga aattattcgc ttcaccaatg aaattgatat
    49261 tcaagtctaa gagatctgtt ttactgttag atgctgtaaa gccaccccaa taatagttca
    49321 caacttctga catcaacctt cttttacttt tttcatcagc tttgatgcgc acgccatttc
    49381 gatctgaaac taaggacatc ccatgcttgt ttagtctttt tctaacatct ttcattgtgc
    49441 tttctaccgt cgacctgctc acgtataatt cgtcagcaag attttgaatc tttagagaat
    49501 cattagtatt caataattta gcaaacacat aagttagacg gtcctccgga gagtctgcga
    49561 tattgatttt atgactggat aataaatttc gaagtgtttc tttttttcca ataagcgaaa
    49621 cgcctttttt aggttgaacc aacatttgta catctggcat tttttggatt atttctttca
    49681 aaattaaaat atctttgcta attgttttcg tagatacata taattgttct gataattctt
    49741 tgtaagttgt gtgctcattt tcaatcaaaa agtatagtat tttcatttgc cgttcgctaa
    49801 attcgatgcc cattatcttc acctcctgaa tacagcttaa gtgtacaatt attatctcta
    49861 ttttcataca gaagaaagtt cttgattaat gtagaagttt ctaatattct tgattatttc
    49921 aaatcaataa aaaagcctgc aatatttata tcacaggctt ttttctctat taaatatata
    49981 taagataata catttttcta ctagttctca ctaatcttgt ataattaaac cctccgcaac
    50041 ttgacggaga tggagaagat gcccccgtct aatctagcgg tcatctctcc tcccattcct
    50101 tccaccaatt tttttgacag gtaaagcccg attccgctag attccgtatt tgtgcggctg
    50161 gtattttcgg tataaaaacg ctggaagact ttttcaacag aggtttgcgt tcctggtttg
    50221 ataggattgg ccactgttaa tcgaatgaat ggatcctctt ttgaaaatgt cacctttgct
    50281 tgtccatctg cataacgcaa taaattctgt atgatatttt gcagcactcg cagcaacatg
    50341 agcggatctg ctaatacagt tgtatttatc gagtgttcag cgaacgctac ctcgatattt
    50401 ttctcagaaa attgttcata aaaagagaaa aatacttctt ccacctcttt agaaaaagaa
    50461 agcgtatgga gatccatttc tttttgcttc gtctctattc gagcaagatc ataaaactga
    50521 tccgtcatac tgagtaatct tgcaacagat tgtttgatga tcgttaaata cttctcccgt
    50581 ttctccgtat cctctacctg ttccagtaat tgtacataac cgttaatagc agtcaaagga
    50641 gttctgagat catgagaaag gccgacgatc gatagtctga tgttttcttc catttcttga
    50701 aatgcttgtc ctttttgttc attttcatcc aaccatgtat tcagtgcatc tactaggtcc
    50761 attagattct tttctcgaaa ttcgactgaa agacgaccac catattcttg tcgttcagaa
    50821 agagtggcga ttttcttagt taatctcttg atttcccttt gcaggaagaa aataaaactt
    50881 cccataaaga taacaacgag aagcaacagc catttaatca aatctaaacc ccactcccca
    50941 caccgtatga atatacacag catgtgcctt tgctagcttc cctcgaagat tggaaatatg
    51001 catattcact gttttctcac tatcaaaata tggttcttgc caaactgttt cataaagatt
    51061 tgctcgcgaa aatacttttt tcggatgttc taaaaaaagc ttcagcagat cgaattctct
    51121 tcctgtcaaa tggacagcca caccattcac atttacatcc ctcgactctt tgtccagtaa
    51181 gatttccttg taatagatgc gttgcgacag atcttttact tggacagaag tctgatgacg
    51241 aagttggatg ccgattcgta acaaaagttc ttcgatatca aatggtttcg tcacatagtc
    51301 atccgctcct gctgccaaca aggaaacttt atctctttga tcgctttttg ctgaaagaac
    51361 gatgatcgga acattcgatg tctgtcggat ttctgctaac aatgcttcac ctgaaagccc
    51421 tggaagcatg agatctagaa ggatcaaatc gacaggatat gtgcttaaca ataattttcc
    51481 ttccgtacct gaataagcat caagaagttt gtatctctgt tcaagaacct cctccagtaa
    51541 ggaatgaaca gcttcatcgt cttcgataat caaaatagtt tccagcagga ccacctactt
    51601 aatgtcagat ttttggaaaa taaacattcc tagagctgat gataccacga ttgttactag
    51661 actgaccaga atcattttcg gtgcttgcga ataagttgct tgttgcgcaa agtccgtata
    51721 catgagccac gtttccgtaa accactccgt tagaaattcc aagcctttga tttttgcgaa
    51781 aacaccggga atgaaggccg ttcccatgat ataaagaaaa gaaagaagca aactgacact
    51841 ggtacttcta gtgatgaatg tcaaagccac tggaacagat aatgtcccta atgtcagcag
    51901 aagcaataca ggaaatactt ttagaacata atttccatag tttttgattc cctcaacttc
    51961 accaaatccg aaagcaagtg tgtaagcaag catggctagt cccgtgatca aaaccacgcc
    52021 aagtgttgtg atcaaaagcg taacgagaag tttcgacaca taataagtca ttcgattccg
    52081 tcctaaagat aacgtattgc gaatcgtacc gttttgaaat tcttccccaa tataaaagca
    52141 cgcaaatgct gcgaaagcca gaagaatcca tgcagccatt ttttctacca ttaaaatccc
    52201 tgttgcaccg ttgatgactg tatccgttgc ttggtttaat tcattgccgt attttgtatg
    52261 catataccat tcgttccaag aataaaaaag cggaagtacg accatcagtc ctagtgagac
    52321 ccacatttta cggctcttcc atacttttat tttttcagca gttaatagat taaacatgtg
    52381 gattgcctcc agttaattgt aagaaataat cttccagttt ttgcccaact aggtgaatac
    52441 catccacagg aacttttgct tctaccaatg cttgattgat ggctgctact tggtcaattc
    52501 cttcaaaaag ctggataaca cggctagatt gtacaaaata atccctataa ccaagagagt
    52561 cgagaaccgt tactgcttgt tccgtcgccg aagtgtcaat ttttataaat tgacgagatt
    52621 cttgtgccaa ttcttctttc gataattctt tgatcaagtg tccttcatgc aaaataccat
    52681 agtgagtggc gatctgtgac aattcatcta gcaagtggct agaaagcaag acggtgatgc
    52741 cccgttcttt taccaaacgt tggatgattt ctcgcatttc tgccatccct gctggatcca
    52801 tcccattcgt cggttcatcc aaaatcaaaa attctggatt cgtgatcagt gcatttgcaa
    52861 tagctaaacg ctggcgcata cctaaagaga aattctttgc tttctttttc cctgtatttt
    52921 ccaatcgcat cattttcagc aggtcttcga tttctctatc cgacacaccg ccgttcgctg
    52981 cttggatacg taggttatct cttgcagtta attctggata aagagcaggg gtttcaatcg
    53041 attgacccat acgtcgtctt gctgcctgca aatccgtcgt tccaaataat tcgatcgaac
    53101 cttcatcaat cgagatcaaa cccataatag ttctcataaa ggtagactta cccgctccat
    53161 tttccccaat caatccatag atcattccac gtttgatttc gattgatact ttatctaatg
    53221 ctttggcagc accgtatttt ttggttatac cagttgcttt caagaccgtt tcatttccaa
    53281 aatccatact tatccttctt tcttattgat gagtgaagta tagttgacgg gtataaagaa
    53341 agcagccaga aatgtataaa gaaagtgtaa attttccaat tgaatgtcaa aaaaaacaat
    53401 cggacaatac gtgtctttcc tattatcagg cagtttgtta tacgcttgta tcagtttcct
    53461 gattctttct tttcgaccaa agtcgtttct ctgtattcct cttcactgaa aatttttgct
    53521 tctttcagat caagcttgag taattttacc agaccggtta taaggataac tatagcaaga
    53581 ctccctccca gaaaaattag actgtctcgc agacgtggtt gcgttgtctt ccaatcaaag
    53641 agcgcaccaa acagtgctaa tccaagtgga ctggccacca tggtaatcgt tgttatcgtt
    53701 gacataagtc gtccttgtat atgcgttgga acttctcgct gaagccagac catataaaaa
    53761 gcaaaagaaa acattaatcc ttaaaaacgg acttatgaaa aataatacat gccgtttggg
    53821 atttacaatt agctaccaaa taagaggtta tgacaaaaga cttttcacaa tcccttcttc
    53881 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt tatcaaacat
    53941 gagaagcaat ttttgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
    54001 tcagaagcgg ataaataata ttgtttgatg atactttatc tttttcccgt ttgtttatag
    54061 atcgaatgat attatagacc aagaatccta cctattaaca tagctaaaaa cacaaaaaag
    54121 gcactttcca aattgtggaa agtgccagaa aagttaggat taaggtaact gcgctcagca
    54181 ttgcttcgcc cagtacgatt ctcgtaactc gtgaactcga acgataatcg caattaacgt
    54241 tttgagaact gtgaagcttt acgggctttc ttaagacctg gttttttctt cgtatcattg
    54301 aataagtttg aaaaaccttg atttataaag gttttcccgt aaatatattt tattatattt
    54361 gattaaattt gaaatgtttg tgttctaaaa tgtgttctaa tttaaaggca gtatatttcg
    54421 tattttagat taaattttta ggctcattca ttct
//
LOCUS       DAHUVV010000007        53328 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.007, whole genome shotgun sequence.
ACCESSION   DAHUVV010000007 DAHUVV010000000
VERSION     DAHUVV010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.007"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(244..2625)
                     /locus_tag="NK767_000420"
     CDS             complement(244..2625)
                     /locus_tag="NK767_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCH6693437.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2615..3262)
                     /gene="recU"
                     /locus_tag="NK767_000421"
     CDS             complement(2615..3262)
                     /gene="recU"
                     /locus_tag="NK767_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCH6693438.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3326..3865
                     /locus_tag="NK767_000422"
     CDS             3326..3865
                     /locus_tag="NK767_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCH6693439.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3955..4374
                     /gene="gpsB"
                     /locus_tag="NK767_000423"
     CDS             3955..4374
                     /gene="gpsB"
                     /locus_tag="NK767_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCH6693440.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4396..4763
                     /gene="rnpB"
                     /locus_tag="NK767_000424"
     ncRNA           4396..4763
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="NK767_000424"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4972..6138
                     /locus_tag="NK767_000425"
     CDS             4972..6138
                     /locus_tag="NK767_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCH6693441.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6184..7680
                     /locus_tag="NK767_000426"
     CDS             6184..7680
                     /locus_tag="NK767_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HCH6693442.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7761..8444
                     /locus_tag="NK767_000427"
     CDS             7761..8444
                     /locus_tag="NK767_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCH6693443.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9089..10279
                     /locus_tag="NK767_000428"
     CDS             9089..10279
                     /locus_tag="NK767_000428"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCH6693444.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10381..11334
                     /locus_tag="NK767_000429"
     CDS             10381..11334
                     /locus_tag="NK767_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCH6693445.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSGNVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11403..12752
                     /locus_tag="NK767_000430"
     CDS             11403..12752
                     /locus_tag="NK767_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6693446.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12935..13258)
                     /locus_tag="NK767_000431"
     CDS             complement(12935..13258)
                     /locus_tag="NK767_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693447.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13387..14550
                     /locus_tag="NK767_000432"
     CDS             13387..14550
                     /locus_tag="NK767_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCH6693448.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14620..15969
                     /locus_tag="NK767_000433"
     CDS             14620..15969
                     /locus_tag="NK767_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCH6693449.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16014..16445)
                     /locus_tag="NK767_000434"
     CDS             complement(16014..16445)
                     /locus_tag="NK767_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6693450.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16833..19475
                     /gene="alaS"
                     /locus_tag="NK767_000435"
     CDS             16833..19475
                     /gene="alaS"
                     /locus_tag="NK767_000435"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCH6693451.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKEHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19675..20271
                     /locus_tag="NK767_000436"
     CDS             19675..20271
                     /locus_tag="NK767_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCH6693452.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20393..21541
                     /locus_tag="NK767_000437"
     CDS             20393..21541
                     /locus_tag="NK767_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCH6693453.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21579..21992)
                     /locus_tag="NK767_000438"
     CDS             complement(21579..21992)
                     /locus_tag="NK767_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCH6693454.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22071..22502
                     /locus_tag="NK767_000439"
     CDS             22071..22502
                     /locus_tag="NK767_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HCH6693455.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22601..22834)
                     /locus_tag="NK767_000440"
     CDS             complement(22601..22834)
                     /locus_tag="NK767_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HCH6693456.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23017..24687
                     /locus_tag="NK767_000441"
     CDS             23017..24687
                     /locus_tag="NK767_000441"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCH6693457.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24834..25220
                     /locus_tag="NK767_000442"
     CDS             24834..25220
                     /locus_tag="NK767_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693458.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25207..25905
                     /locus_tag="NK767_000443"
     CDS             25207..25905
                     /locus_tag="NK767_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCH6693459.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25910..26374
                     /locus_tag="NK767_000444"
     CDS             25910..26374
                     /locus_tag="NK767_000444"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCH6693460.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26371..27282
                     /locus_tag="NK767_000445"
     CDS             26371..27282
                     /locus_tag="NK767_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCH6693461.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27517..28056
                     /gene="pyrR"
                     /locus_tag="NK767_000446"
     CDS             27517..28056
                     /gene="pyrR"
                     /locus_tag="NK767_000446"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCH6693462.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28070..29353
                     /locus_tag="NK767_000447"
     CDS             28070..29353
                     /locus_tag="NK767_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCH6693463.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29366..30292
                     /locus_tag="NK767_000448"
     CDS             29366..30292
                     /locus_tag="NK767_000448"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCH6693464.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30311..31591
                     /locus_tag="NK767_000449"
     CDS             30311..31591
                     /locus_tag="NK767_000449"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCH6693465.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31596..32672
                     /gene="carA"
                     /locus_tag="NK767_000450"
     CDS             31596..32672
                     /gene="carA"
                     /locus_tag="NK767_000450"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCH6693466.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32677..35862
                     /gene="carB"
                     /locus_tag="NK767_000451"
     CDS             32677..35862
                     /gene="carB"
                     /locus_tag="NK767_000451"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCH6693467.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35916..36692
                     /locus_tag="NK767_000452"
     CDS             35916..36692
                     /locus_tag="NK767_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HCH6693468.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36689..37621
                     /locus_tag="NK767_000453"
     CDS             36689..37621
                     /locus_tag="NK767_000453"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCH6693469.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37618..38334
                     /gene="pyrF"
                     /locus_tag="NK767_000454"
     CDS             37618..38334
                     /gene="pyrF"
                     /locus_tag="NK767_000454"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCH6693470.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38341..38970
                     /locus_tag="NK767_000455"
     CDS             38341..38970
                     /locus_tag="NK767_000455"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCH6693471.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            38958..39272
                     /locus_tag="NK767_000456"
     CDS             38958..39272
                     /locus_tag="NK767_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693472.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39310..39951
                     /locus_tag="NK767_000457"
     CDS             39310..39951
                     /locus_tag="NK767_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCH6693473.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39994..40686
                     /locus_tag="NK767_000458"
     CDS             39994..40686
                     /locus_tag="NK767_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HCH6693474.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40683..41585
                     /locus_tag="NK767_000459"
     CDS             40683..41585
                     /locus_tag="NK767_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCH6693475.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41743..43449)
                     /gene="efbA"
                     /locus_tag="NK767_000460"
     CDS             complement(41743..43449)
                     /gene="efbA"
                     /locus_tag="NK767_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HCH6693476.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            43639..44262
                     /locus_tag="NK767_000461"
     CDS             43639..44262
                     /locus_tag="NK767_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693477.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            44712..45761
                     /locus_tag="NK767_000462"
     CDS             44712..45761
                     /locus_tag="NK767_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCH6693478.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            45758..46639
                     /locus_tag="NK767_000463"
     CDS             45758..46639
                     /locus_tag="NK767_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6693479.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            46642..47412
                     /locus_tag="NK767_000464"
     CDS             46642..47412
                     /locus_tag="NK767_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6693480.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            47691..48545
                     /gene="aroE"
                     /locus_tag="NK767_000465"
     CDS             47691..48545
                     /gene="aroE"
                     /locus_tag="NK767_000465"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCH6693481.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            48723..49886
                     /locus_tag="NK767_000466"
     CDS             48723..49886
                     /locus_tag="NK767_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCH6693482.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            50003..50413
                     /locus_tag="NK767_000467"
     CDS             50003..50413
                     /locus_tag="NK767_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCH6693483.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            50587..52815
                     /gene="topB"
                     /locus_tag="NK767_000468"
     CDS             50587..52815
                     /gene="topB"
                     /locus_tag="NK767_000468"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCH6693484.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            52986..53270
                     /locus_tag="NK767_000469"
     CDS             52986..53270
                     /locus_tag="NK767_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693485.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT        17341 a         9335 c        11565 g        15087 t
ORIGIN      
        1 aataaaagac tagtgtgtat ttagccgatt taattataaa caagtaaatg gataaagtat
       61 atgtaatgat aaaagaaaaa tggattttct aatttttaag ctaaatgtta gatcacacat
      121 gtatatgctg gatagtctta taaaaaatca tgtaatacag cttggtacat gatttttatt
      181 tgtagctgtt ttttacatag acacaaaaaa gaaaccacgc ttgtagacgt ggtttctttt
      241 ttactatctg tttccagaag ctgaggtgct gctggatggt gtatggtcat ttgcagcatc
      301 cgctccgcta gttgctggct cactggaaga agcaggagga gcagatgaag acggtgtcga
      361 gctttcttcg gatgaagaag aaggaaccgt tgttgaagac gctggctcgc ttgtttctgc
      421 tgtcgtgctt tcttttgagg actcagcagt tgattcgctt tgactagtag agctagagct
      481 ttgagtcgtt gtctctgttg ttgagcttcc aggagtctgg acatagctgg aagatggaat
      541 agttgttgat ggtgtaattg cattcgatct agctgtatat tgatctttgt agtacagttc
      601 accgcccaca cgaattaatc cgcttggcat ctcccagtct gtatttgtca cgttagctga
      661 aacatactgc ataagctcgc gataaacatc agaggctacg tgtgacgatt ctgaagtgac
      721 aggtgtcatt tttttattgt aacctgtcca cacggaaatc gaataatttg gcgtatagcc
      781 cgcaaatagg atatctggat acaccccaga agaaatacca agttttgcat actcgtcatc
      841 tgtgtaattg gatgttccgg tcttaccggc ttgatataac ccagcgattt gcgcattcgt
      901 accagttcct tcagtgatcg tatcttttaa aatgtctgtg atcatataag ctgtttctgg
      961 agacatggct gtttttccat ctggctcata agtttcttct gttccgtctt ggaagacgat
     1021 tttattaaca tattgaggtt tgtaataagt ccctccattg gcgaaagccg cataggctgc
     1081 tgccattttt aaggaagaag ctccgtattt tgtcccatct tgttcttccg tattactaga
     1141 gatcgcattc gattgatgga tcgtactgta ttcgatccca agatttttta agaaagaagc
     1201 cactttatct gaaccgactt cattgaataa tttgactgct ggaacattac gggataagta
     1261 aagtgcttgg cgtaaagtaa tggtgcccat atattgatta tcccagtttc ctacaggagt
     1321 cgaagtccct tcatagttat acggtgcgtc agtaatagtt tttccagtag aatatttcaa
     1381 atattcaaaa gctggtccgt aatctgtgac aggtttcatc gtagaaccaa aatcacggga
     1441 agtattcact gctaagttgt tgcctaaagt tacgtcttct gcgatgtgac gtccgccaat
     1501 ctgggctttt actttcccgg tattggtgtc gataagggtg gaggcaactt gcatctcgtc
     1561 gtctgggtaa gagacatact gatccgtgtt gacgatatca tatagtttct tttgggcgtc
     1621 cagatccaaa ttcgtataaa tctctaaacc gtctgtgtag acattcttat ctgttttttc
     1681 ttgtacttca ttgatgactt ctttgacata attatcgacg atcttagtat tgtcatcgga
     1741 ttgagttagt tcttgcagac catccgttac cggtacatta acagcttgat catattctgt
     1801 ttgagagatt ttttcgtttt gaagcatcgt atacaaaaca gtatctcgtc ttttcttcgc
     1861 ctgatctgga taaacataag gatcataggc ggaaggggct tgaggcatcc cagcaagcaa
     1921 tgctgtttgc ggtaaagaaa gttctgataa tttcttacca aagtacatct cagaagctgt
     1981 ctccatcccg tacaagccgt ttgacatata cactttattt acgtagtagg tcaagatctc
     2041 ttgtttcgat tttttttgtt ccaatcttac agccatccac gcttcttggg ccttgcgttt
     2101 cagagtctga tcctcggcac tggtggagaa gaaggaaagt ttgatcaatt gctgtgtcaa
     2161 agtacttccg ccttgcagtc cgccagaggt aaagttagag agggcagaac cgatgatccg
     2221 aatagggtca accccgatgt gtttataaaa acggcgatct tctaccgaca cgatcgcatc
     2281 ttctaatgtt ttcggtaatt cattcgcaga aattttttca cgcttttctg cgcctaaatc
     2341 ttcgaatagt tcgccatctt gtgtatatag tttggaagat acggtagcgt ctaatttttt
     2401 atccgtcaat tcgggcgcat cttttgcgta ataccagaaa agtcctacac ctgctaaaaa
     2461 gaggatacag aaaaatgaga gtattcctaa aaggatcttg ataaataatc cttttttatg
     2521 tgttccagag gactttccag aacctttgcc agaattcttc ttcacactct ttttaggagt
     2581 aggctgtttc tgtcgtctag agcttctagt ttgttcattt gccattcttg ctttctcctt
     2641 caatagataa tgttgataaa tagaagtcaa ccgcgtccaa ataaggaatc cgcggagaaa
     2701 taccagtttt gatctctatt cctagttctt cgatcaaaga taaaggtagt gatttgcgtc
     2761 ctttttcctt ttgggcgtcc cagtaagcaa tcaaatgaac actattcaaa taaaaacagc
     2821 gcttcaaaga cgaaaaccat aacaagacaa agcagacccc attttgttcg aggcatgctt
     2881 tcatatgttc gatttgatgg gcatggaagt ttttaaaggg aaaagatgtt ttgttcttgg
     2941 tttcttttgc ttcaaagtcc aagtaaaagc ctcgatagac gccattatag tctgtagttg
     3001 aggcttgggt aaagtaggct tccttgatca cagcagcact tctcttagga tagtctactt
     3061 tcacgatttg gataggggta ggctttttat ggataaccgc caacccgcgg gaaagataat
     3121 atttattact ttcattgatt gcttcttcaa agcgcatccc ccgattgccg aattcagtga
     3181 tctgatttct tttttcttga attttttcga cgggagaggc attcttataa taaggttggc
     3241 catttggata gcgcaatacc atctttaatc acactcctag gccacattat agcaaaaaag
     3301 cggatagaaa gaaaaggaat ctgagatgga taagtcaaaa gtaatctatg tgtctgggta
     3361 tcgaagtttc gaactcggtg tttttaaaga aaatgatcct aaaatccaag ttgttaaaaa
     3421 tgttttaaaa aaagaactcc atcagttagc agaagaaggg ctggaatggg tacttgtcag
     3481 tgggaatttg ggcgtcgaac tatgggctgt gcaaatagta gcggaattga aagaagaata
     3541 tccggaacta tctcttggta ttttgtatcc tttttctgat tttggtaata actggaatga
     3601 agggaaccaa gaaaaaaaac aattagcaga acaattagca gattatgtcg aagctgttag
     3661 ccatcaaccc taccaatcac ctagtcagct aaagaatcac actcgtttta tacttgagca
     3721 tacagtgggc tgtctgctga tctatgatga agaattccct ggaaaaacga agtatttttt
     3781 tgaagatgca cagaattacc aagcagatca tccctacgaa attcggctga tcagtatgga
     3841 cgatcttcaa aattttagtg aataatcgat attgtgtttg atttatcaac ttttttccta
     3901 tacaatgaaa aaggtagaaa tgttattacg taaatgaaaa ctagaggtgt aaatatggcg
     3961 aatttggttt atagtcctaa agacatttta caacaagaat tcaaaacaaa aatgcgcggc
     4021 tatgatcccg ttgaagttga tgaattctta gacaatatca tcaaagatta tgaaacttac
     4081 agcaaagaac tccttgcttt acaagaagaa aacgatcgat tgagtgcaaa agtagctcaa
     4141 ttatctaaaa ctcaaggagc agctcaaaca cgcgtgcaac aaacagaggc accaaagagc
     4201 gcagcagtga ccaattttga tatcttaaaa cgcttgtcaa atcttgaacg tgaagtattt
     4261 ggtaaaaagc ttgatcaaca agcaagtgca gtcaaaccag cacaacctaa tccaaataac
     4321 tacacaaatg cagacacaag cttagatgat aatgaaaaaa cacgtcaatt ttaatttttg
     4381 attaatgaat ttgcggtttt cgggtaatcg cggtctgact tgatcaggct gaggaaagtc
     4441 catgctcgca caggctgtga tgcctgtagt gttcgtgctt agcgaaacca taagctaagg
     4501 tactctttgg agtaacggca ggaaaacaag ctaaggcttc ggctatgctt aagtatcctt
     4561 gaaagtgcca cagtgacgaa gcggctgggg aaacccatcc ggtggaacgc ggtaaacccc
     4621 tcgagcgagc aacccaaaca atggtagggg cgctttttgt ctggaagaag aacaggacaa
     4681 aaaggcagta taactgcaga tagatggtta ccgctttgtt ttttctccct ggaaaagcaa
     4741 agtacagaac atggcttata gaaaaccgca aacatcaggg aattaggtcg tgacaaaagt
     4801 aatgcgttat aagaaacaag aaaaacattt gaaagctatt tttcaatact tcagatgaat
     4861 cgttcttatt acaaaacaaa cttttgtatc accggttatt cggaatttga gtaaaagtgt
     4921 ggggtaggac tgatgtccca cctccttttt ttataaataa ggagattgtg tatggaaaaa
     4981 aacaggaagt tcaacctcgt cgcaactgcg gcaagcgggc tagaagcact ggttggtaaa
     5041 gaattaagag atcttggaat cgaatgccaa gttgagaacg gacgtgcacg ttttgaagga
     5101 acaatggaaa cgatcgctac agctaattta tggttacgta cagcagatcg agtaaaaatc
     5161 gtggttgggg aattcgatgc gtatacgttt gatgaattgt ttgaaaacgt aaaagcattg
     5221 ccttgggaag attttcttcc actagatgca gcttttcctg tagctggaaa atcgatcaag
     5281 tcgaaactat atagcacacc tgattgccaa gcgatcacaa aaaaagcaat cgttgaacgt
     5341 ctccgtacct attatcaccg tccagcttct gtaccactga cggaaactgg cgcacatttt
     5401 cagttggaag tagctttgct gaaagaccat gtgatggtaa ctctggatac gacaggacca
     5461 agtttattca aacgtggtta tcgcttagaa aaaggtggag caccattgaa agaaaacatg
     5521 gcagcagcac ttattatgtt gacaaattgg cgaaaagacc gtccatttta tgatcctgta
     5581 tgtggttcag gtacgctttg tattgaagca gccttaatag gccataatat cgcaccagga
     5641 ttcaatcgtg agtttgcttg cgaatcttgg gattggttca gtcaagaaat catggaaaat
     5701 gttcgatcag cagccgaaga aaaagcagat tatgatattg aattagatat tactggttca
     5761 gatatcaatg gacggatgat tgaaattgcc aaagccaatg cagaagaaat cggattaggc
     5821 tcttcgatta cctttaagca acaagcagtc aaagacttca aaacggataa agaatacggg
     5881 gtcatcgtgg ctaatccgcc ttacggagaa cgtctaggag aagaagaaac tgttcggaaa
     5941 ttatataaag aaatgggcga tgtattccgt ccattaaaaa catggagtaa atatatcttg
     6001 actagcgact tagcgtttga ggaattttac ggacaaaaag caaccaaaaa aagaaaattg
     6061 tacaatggtg cattgcgtac ggatctattc cagtattggg gaactcgtcc gcctagaaaa
     6121 aaagagaaag acgaataatc ttactaattg attcattcaa ttggatgttt ggaggaatag
     6181 ggaatgaaag aaaaagagtt tttacaagaa gtaaaagaaa tcaatcttct acaacaagct
     6241 ttaggaatcc tcgattggga tacacaaaca ggtatgcctg aaaaagctag tgcataccga
     6301 agtgaagtag acagctatct ttacagcatt tatttttcaa aaaaaatcgg tccaaaaatc
     6361 aaagaagcca ttcgctattt ttctgaacat ccggaagaat tgtcggaagt tggaactgcc
     6421 gtttttgcca aagtaaaaga agagtatgat ttggaacatc gcgtaccgga agatcttatg
     6481 agaaaatata cagctgccgt ttcttcagca cacggacaat ggcaaaaagc tagagaaaca
     6541 caacaatttt ctgattttca acacgcattg aatcaaaata ttgaattaac aaaacagttg
     6601 ataccttact ggagaaaaga tgaaaagacg aattatgatg ttttgctgaa tcaatacgaa
     6661 cccgggatga ctgtcgaaat ccttgataag gtatttgatc agcttaaaga aggaattctt
     6721 tcgatccgcc gtacgctcca agaaaaaggg actgaacctg aaacagactt tttaaatcgg
     6781 agaatgacga aaaaacaaca gaagagattt gtagtgaaag taatcgaaca acttggttat
     6841 gattttagtc gtggtcgttt agatgacact gtccacccat ttatgactgg aattaatccg
     6901 aatgatgcac gaatcacgac acgatggaac gaaacagatt tcaaaatggc tgtttttgga
     6961 gtgatccacg aggctggtca tggtatgtat gaacaagaca tcgatgataa atatgcgtat
     7021 acaccggttt atgaaggtac atcaatgggt atccatgaat cacaatcgtt gtttaacgag
     7081 atcattattg gaagtagtcg cagcttctgg aaaaagcaat atcctttctt tcaagaatgt
     7141 gcagaaggaa cattcgatga tattcctttt gacaaatttt atcgttcatt gaaccataca
     7201 aaatcaagtt tggttcgtat cgaggcagac agtttgactt accctctcca tatcatcatt
     7261 cgttatgaaa tcgaaaaaat gattttcaat gaaggggtag atgttgctga actacctgaa
     7321 atatggaatc aaaaatatga agagtatctg ggaatacgac caacaaatga tgtggaaggc
     7381 atcctacaag acgttcattg gtcaggtgca agctttggtt attttccctc ttatgcatta
     7441 gggtacatgt atgcggcaca attacttcat tctttgcagc aagaaataga tgtggaaaat
     7501 gttctagcgt cagacgatta ttctccaatc aaaaactgga tgtcagagca tatccatcaa
     7561 tatggtgcat ctagaaaacc aaatcaatta atcatggatg caacacatga accgcttaat
     7621 ccgcaatacc tgatcgacta tatgaaagac ctgtatttta atgtatatca ggtaaaataa
     7681 aatacaattt acaaagatag ctgaaattca tctaatctat agatgttttc agctatttac
     7741 ttaagaaaga agtggaacga atggataaat tacaagtgat cgctgattat agtatggacc
     7801 aattagcgca tgatcaaaca ggacatggga gcgatcatac caaaagagtt gtcaagttag
     7861 ctgaaaaaat tttggagacg gaaccgcagg cagaccgctt tgtgacgctt gcagcagctt
     7921 acctacatga tacgattgat gataaagtcg ttaaaaatga gaacgaggca aagcaacagc
     7981 ttcgtgtatt ccttcgctct ttgccaatag tagaggaaca gatctcgatg attttttcca
     8041 ttatcgaaaa tatgtccttt tctaaaaatc tctctgaagc ggccgaacta tctctggaag
     8101 gtaagatcgt tcaagacgct gatcgtatcg aagcattagg agcaatcggc attttgcgta
     8161 ctgcttattt cggcggtgga catgggcacc cgatttttga tcctgaactg tatccgcaaa
     8221 cattcaagga caaagaaaat taccgaaaag gcacgacagt gatcaatcat ttttatgaaa
     8281 aactgttctt gctgtctgat aaaatgaata ctgattacgg atatgaagaa gccaaacgaa
     8341 gagaaacctt catgcgtgaa ttcttagaag aattttttct ggaatggtct attgacgatg
     8401 aaaattttac tccagacaat tggatcaaaa ataacaaaaa atgatggagg ttgtgacaga
     8461 agtggtcagc tccaagatat ttcggatcat tttccgtaaa agttacttct ggaacactgt
     8521 ttaccaagag gtcgtgagcc tgccgttcag ctccgagtca caaggaacaa tttcaaaaaa
     8581 tagcttctcg tgtttgatga atattgtaac ttgtgattcg gaattggcag gagaacaccg
     8641 tttattcgga agaataggac tgtgacaaga cttttgtcac agtccttttt tcgaattcaa
     8701 caagtaaaac ttttaagaaa tattcagaga gtaaaattaa aaattgctga taaacgaaga
     8761 tggaaatttt atgtattttt attggtctat gcaataaata aattaaatat tcagaataca
     8821 attgatttta acgaaagggc ttctatcaat ggttgtgttg tatttatctg cccaggtaga
     8881 gtaaaataga tcattatcta aaaggtaatt gttcatacta tataagtcat ttgaaagtaa
     8941 aaagatactg taaataaaaa atgtggttaa ataattctca taatcagtgt aagcgttatc
     9001 aaaaatatag catacttgct ttgtagatga atgacacgat atatgtttgg tatgttctgc
     9061 aagacagatg aatatggagg tggagacaat gtcttccaaa gaactaacaa tgtatttaga
     9121 gaaagaattg gatcaactga aagaaaaggg gttatacaat acgatcgaca tactagaaag
     9181 tgaaaatggt gcttgtatca tcgtagacgg aaaaaagatg atcaatctag catccaacaa
     9241 ttatcttgga tttgccaatc gcgaagaatt gaaaaaagca tgtattgaag caaccgaaac
     9301 ttatggtgta ggagcaggag cagtccgaac gatcaatggt tcgctgaaaa tccatcagca
     9361 attagaagaa aaaatcgcag aattcaaagg gacagaagca gcaatcgcct ttcaatctgg
     9421 attcaactgc aatatgggcg ctatctctgc agtaatgaca aaagaagatg ctatcttatc
     9481 ggatgaatta aaccatgcat ctattattga cggttgccgc ttatccggag caaaaatcat
     9541 ccgaattaaa catcaagata tgaaagacct tgagaaaaaa gcaaaggaag cgatagaaag
     9601 taagaaatat aaaaaaatca tgtatatcac agatggtgtt ttctccatgg atggagacat
     9661 tgcacgattg ccggaaatta ttccgattgt tgaaaaatat ggactgatca cctacgtcga
     9721 tgatgcgcac ggctcaggcg taactggaaa aggtgcagga accgtcaagc attttggatt
     9781 aagcgacaaa atcgatatgc aaatgggcac gttatcgaaa gctataggtg ttgttggggg
     9841 ttatgtagct ggatcgaaaa cactaatcga ctggttgaaa gctagaagtc ggccattcct
     9901 attttctact tcgctaactc caggtgcagc tgcagctgca ttggcttcga ttactttgat
     9961 gcaagaacat ccggaacttg tcgaaaaagt ttgggaaaat gctaattatt ttaaagaaga
    10021 attgaaaaaa gtaggctata acatcggtat gtctgaaaca cctattactc cagtcatttt
    10081 gggagacgaa aaagtgactc agactttctc taagaagctg atcgaacatg ggatttatgc
    10141 taaaccaatc gtttatccaa ctgtgcctct tgggacaggc aggatcagaa atatgccgac
    10201 tgcggaacat acgagggaaa tgcttgatga agcagtagca gtttatcaaa aaataggaaa
    10261 agaaatggaa attatttaac taaatttagt tttttcgtct atttaaaatt agagaacatg
    10321 ttcacgcata tattgttaat catctgaaga tagtagtaaa aaagatcagg gaggatcatc
    10381 atgaaaaaag tattggtgac aggatgttta ggacaaattg gttcagagtt gatcacacgg
    10441 ctacgtcaag aactaggcag tggaaatgtc attgcgacag atatacgcat gcctgaagtc
    10501 attgagaaaa acgatatctt tgagatccta gatgtaatgg attacgaaaa aatgcgcagc
    10561 atagcagaac aatatcaagt ggacacattg attcatttgg ctgctctttt gtcagctgtt
    10621 gcagaagcaa aaccacaatt agcctggaat ttgaacgtga ctgggctggt caatgcattg
    10681 gaagtcgcta gagaattcca gctgaaattt tttacgccaa gttcaatcgg cgcgtttggt
    10741 ccaaatactc caaaagatca taccccacaa gatactcttc agcgtcctac aaccatgtat
    10801 ggggtaacga aggtagctgg agaactacta tgtgattatt attatgtgaa atatggcgta
    10861 gatactcgtg gtgtccgttt ccctggattg atttcttatg caaccttgcc aggaggcggt
    10921 acgacagact acgcagtcga tatttattat tcagcaatca aagaaggaaa gtacactagt
    10981 ttcattgatc aaggtacatc aatggatatg atgtatatgc cagatgcgat cgatgccatt
    11041 ataaaactca tgaatgcaga tcctgatcga ttggttcatc gaaatgcctt caatatatcg
    11101 gcgatgtctt ttgacccgga agagatcaaa gcatctattc aaaaaatcat accagatttt
    11161 gaaatggatt atgatgtgga tcctgtacgt cagtcaatag cagaatcttg gccaaattca
    11221 ctagatgttt cttgtgcgca agaggaatgg ggcttttcgc cgaaatacga cttagatgtg
    11281 atgacaaagg atatgttgga aaaactgaaa gaaaaattgg tagagacggt ataaaacaag
    11341 aaccacatcc aaaaagtttt ttatactttt gggattggct atggtatgaa aggggacaag
    11401 ggatggaaga atatcaagcc acgccagtaa aagaagtaaa aatagaaaat gaattaaaac
    11461 gtacgatggg attttttact gctttatcga ctgttatggg cacagtgatc ggtgcagggg
    11521 tattttttaa agccgcaagc gtagcagaag ttacaggatc cgccagtttg catatgtttt
    11581 cttggtttct cggtggtatg atttctgtct gtgcaggact aacaggagct gaactggcag
    11641 ctgctattcc agaaacagga gggatgatta aatatatcga acggatttac ggaaatacag
    11701 cagctttttt attgggctgg gcgcaagtcg tgatttattt tccagcaaat gtagcagcct
    11761 tgtcgatcat tttcggtaca cagttcgtca atctatttgg tctttcccag tcaatgattg
    11821 ttcccgtagc tgttacagct gctgtatcga tcctgttgat caatttttta ggttctaaag
    11881 ctggaggggc ttttcagtcg attacgcttg tctgcaagct tattcctttg tttgtgatcg
    11941 tcattttcgg tttgttccga caagaaggag tagacttcca actgtttccg atccaagcag
    12001 gagaaaatct ttcatttttc tcagccttgg gagcgggctt gctagcaacg atgttcgcat
    12061 acgacggttg gatacacgta ggaaatattt cgggagaatt gaaaaagccg gcgaaagatt
    12121 tgccaaaagc aatttcttta ggaatcatcg gaatcatgat cgtttattta ttagtaaatg
    12181 cggtgttctt aagaaccgca tctatcgacg gagtcgcagg aaacagcaat gcggcaagtg
    12241 acgttgcaaa aatgattttt ggcggcttcg gtggaagact ggtcacagtt ggtatcttga
    12301 tttctgttta tggaaccatc aatggctata cccttactgg aatgcgtctc ccttatgtta
    12361 tggcaaaaga aaacaacctg ccttttagca agctctttgc taaacttcat gataaaacta
    12421 aagttcctgt agccgcaggt atcttagagc tagtcatcgc tattggcatg atgatgatcg
    12481 gtggttttga tacactaacg gatatgctga tctttgtcat ttggattttt tatacgatgg
    12541 tctttgttgg tgtcattctt cttcgtaaaa aagaacctga tttgtttcga ccatacaaag
    12601 ttccaatgta tccgtttatt ccactagtag caatcattgg agggactttt atcgtatcaa
    12661 gcacgcttat cactcagacc tttttagctt cgatggggat agtattgaca cttgctggta
    12721 tcccgattta tctctattta aaacgtagat agctaaaaca ttcgaatttt attagaactg
    12781 cacttctctg ccaaacaaaa tattttattt aaatgaggag tacgtggaag aaatgtcaaa
    12841 agtactcgtt aattttgatc acgtaaatgt cgttttgtct acgatgaaat agcaaagtaa
    12901 aagacagaaa tcgggctcaa ccgatttctg tcttttactt tgatttgcgt aactgaacta
    12961 ttcggcgtac gccgcgataa atatttaata aaaccaagca accagaaatg aaaatgggaa
    13021 gccaaatcag atagacaggg tagccaagca ctttagacaa agcatgtgga gtcattttat
    13081 ccatttcaag aagtgcttgc tgccaagtct gaagattgaa gactagcatg aaacccgaaa
    13141 taatcaggag aataaatcct aaataatgaa ttgtagttct ttttcttaca taagcgatcg
    13201 tagcataaag gaacagtaaa aaaataggta cgccaaaaac aaagtacata ttcagcatct
    13261 cgacaccccc tttgtgttta tcttacttta ctttgaaaga aatgagaaat aatagcctcg
    13321 ttacttgctt gaaacagcaa aggactgcta agataagaga gatacaaaga atcaaggagg
    13381 agtcatatgg caagttatat cgaaccgatt cgttttgcgc tgattgtttt tccattttta
    13441 gccttagcga tctcatctgt tttttttatt tatgaatatc gaaagtatgg aacatttcta
    13501 gtaacaaggg cagttatttt gtattctttc gttttttatt tattgtgtgc gtattttttg
    13561 gtgatcttac cacttccggc aaaggagacg gttgcccaaa tgacagggcc aaagatggag
    13621 ctgcatttat ttgcttcatg gcaacatttc agagacaaca cggtattggt cttgtctgaa
    13681 cctagtacct acctgccggc aatgaagcag agtgtatttt tggaaccagc ttttaatgtt
    13741 ttattactca taccatttgg catttattta agatattatt tccggctttc ttttatgaaa
    13801 acgatgattg ctagtttctg cctttctcta ttttttgaat tgacgcagct aagtggactt
    13861 tattttatct acccaagacc ataccgctta tttgatgtga atgatttagt gacaaataca
    13921 ttgggtggaa tcattggttt tgtcgttacg ccgttattta catttatgct tcctacaaga
    13981 agacgaatgg atgaactttc ttatgaaaaa ggaaagactg tctcattgac tcgacgcttg
    14041 gtcgcttatt tgatcgactg gacattttta tctcttatta atttggtagc ggcgattttc
    14101 tttaaaattg cgttcgattc aggggcagtt acttctcata tctggtggct tttattagaa
    14161 gtaggggtat actttatgat tgtaccgttt ataaccaatg gatggacact tggcaaaaaa
    14221 attgtgcgta tccgtttggt ggaagaagac cgcgaacgaa tttctttaaa agcattgatg
    14281 atccgctacg ggtatctgta ctttcttttt tatggtttaa gctggttggg gcagttgctt
    14341 cagcaaatca tgaattcgac aacgaatacg gtgttgcaga tgagtgcagt attcatttct
    14401 ctcggtgtct tgttactaca aggtattttt attttaaata tcttgctatc atttttgaga
    14461 aaaaaacgga ttctgtttta tgaaaaagct agtcacacat atactgttag cacgattggc
    14521 caaacaaagg atgatgattc tcgaaattag ctgagcaaaa acgctttgtc tgtgttataa
    14581 tgccaaagtc gtgataattt aaagagaaga ggtgcacgaa tgaataattt tgatatattc
    14641 cgcttcaaac cgtttatcaa tgaagcgtta accgcaaagg gcttcaaaca gcccacagag
    14701 gtccaggaac gtttgattcc tttaattgca aaaggaaaaa atatcattgg gcaatctcaa
    14761 actggtacag gaaaaacaca tacgtttttg ttgcctttgt ttgaaaaaat caatgcagaa
    14821 aaagaggaag tacaagttgt tattacagct ccaagtaggg aactagctgc acagatctat
    14881 gaagcagcta cacaaatcgc taaattttct acaccggaga tccgtgtctc gaattttgtc
    14941 ggcggtacag acaaacagcg tcaattaaat cgtctgcatc accaacagcc acaggttgta
    15001 gtaggcacgc ctggtagaat attagacatg atgaatgaac aagcgctgaa gattcacacc
    15061 gcatttgcat ttgttgtcga tgaagcggat atgacattgg atatgggttt tttaaatgaa
    15121 gtagaccaaa ttgcaagtcg attaccagaa aagctgcagt ttttagtttt ttctgccact
    15181 attccagaaa aacttcgtcc attcttacga aaatacatgg aaaatccaat cattgaagaa
    15241 atcaaaccaa aagcagttat ttctgaagac attgaaaatt ggctgatttc aacaaaaggt
    15301 aaagatgaga atcaaatgat ttatcaactg ctgacaattg gtcatcctta tttagcaatc
    15361 gtatttgcca atacaaaaca gcatgtggat gaaattgccg attacttgaa aaaccaaggc
    15421 ttgaaagtag caaagattca tggagacatc accccaagag agagaaaacg agtgatgcga
    15481 caagtacaga atctggagta tcaatatgtt gtagcgactg atttagctgc tagagggatc
    15541 gatatcgaag gtgtctctca tgttattaat gcagaaattc cagaagatct agacttcttt
    15601 atccatcgtg tcggacgtac tggacgtaat ggcttaaaag gaacggccat tacgttatat
    15661 tcgccaaatg atgagcaagc cattgatgcg atagaaaaat tgggcgtctc atttgaacca
    15721 aaagaaatca aaaacgggga aatcgtcaaa acatacgatc gtaatagacg tgccaaacga
    15781 gaaaaatcaa aagatgtatt agatcccacg ctgattggtc ttgtgaagaa gaaaaagaaa
    15841 aagatcaagc ctggttataa gaaaaaaatc gattgggcaa ttgcagaaaa aaataaaaaa
    15901 gaacgcaaaa tcgaacgcag acagcaaagc cgtactgctc gtaaaaataa gaaaaattca
    15961 cataaataaa aaacaagagg ttgtgacaaa aggcgtgtca caatctcttg tttttaagct
    16021 agactttttt tcattccgat acaaagtact tcatcttcaa taaaaggttc tgtgcaagta
    16081 tgataccctt gtttttcata aaaatctaca gccgtcattt cagctactac ataactagtc
    16141 tgacaacctt ctgaaaccgc ccgtttttca gcagcttcaa gcaactgacc gcctaaccct
    16201 tttcctcggt attcttttgc tacgcaaaat cgttccggct tgaagcaagt aggtgttttc
    16261 ttttcataac gcaaagtagc tactggtctt ttttgatcaa gaaatagata ataggtttgt
    16321 tgcgggatat cttttccgtc gaattctagt tctgcaggaa tcccttgttc taatacaaaa
    16381 acttgatatc tcaaaaagca gctagctgcg cgtatccatt catccgctcc aactattttt
    16441 ttcatcatat tcctccttgt catgaatagc agagatgtat tgattccttt attcttatcg
    16501 taccaaaaat cgtggagtat gaccaaaagg aaatgaaaat aaaaattgag atattgacag
    16561 tgatcaatag aatgcgtata ctaaataagt acgaaaagga tatgatttgt tttttaagac
    16621 tcacagaaaa ttctttcttg gctgaaaaaa gaatagtcac taatcaaatt gctccctttt
    16681 tctattttgg tagaaatacc gacgcagctc agcgttatcg agtttaagag gtaatgatta
    16741 cacatcattg caatcaaggt ggtaccgcga gttttcgtcc ttggctgtga tggtgttttt
    16801 ttgtatttaa tttatagaaa gcaggtaatg acatgaaaca attaagtagt gcacaagttc
    16861 gacaaatgtt tcttgatttc ttccaatcta aagaacatac gattgaacct agtgcgtctt
    16921 tgatccccgt gaacgatcca accttattgt ggatcaactc tggtgttgca acattgaaaa
    16981 aatattttga tggttcagtt gtgccagaaa atccaagaat cacaaatgcc caaaaatcta
    17041 ttcgaacaaa tgatatcgaa aatgtcggta aaacagcacg gcaccatacg atgtttgaga
    17101 tgctgggtaa tttttcgatt ggtgactatt tcaaaaatga agccatccac tgggcatggg
    17161 aatttttgac tgatccaaaa tggatggcat tcgatccaga aaaactttat gtaacagttt
    17221 atccaaaaga tacagaagca aaacgcatct ggcatgaaga agttggcctg ccactagatc
    17281 acattattga tgtagaagac aacttctggg atatcggtgc aggcccatgt ggtccggata
    17341 cggaaatttt ctatgaccgt ggagaagctt ataacgattt agcagaagac gatcctgaaa
    17401 actatccagg aggagaaaat gaacgttatt tagaaatctg gaacttagta ttttcagaat
    17461 tcaaccataa gcctgataac acatttgaac cacttcctca taaaaacatt gatacgggga
    17521 tgggattgga acgcatggtc tcaatcgttc aagatgcgtc aacgaacttt gagacagact
    17581 tgtttatgcc tattatccac gccgtagaaa agctaagcaa acaagtaaaa tatggtgaaa
    17641 acccagtaac agatatttct tacaaagtaa tcgcagatca tattcgcgcc ctttcatttg
    17701 ctattggcga cggtgcattg ccatcgaatg aagggagagg gtatgtctta cgccgtttgc
    17761 ttcgtcgtgc tgtcatgcat gggaaaaaat taggtattga acaagcattc ttatatcgtt
    17821 tagtccctgt tgttggggaa atcatggtaa gttattatcc tgaagtactt caacaaaaag
    17881 aatttattga aaaagttatc cgtacagaag aagaacgttt caatgaaaca atcaatgaag
    17941 gtttggaaat cttaaatcaa gtgatccaag aagtaaaaaa cgaaaacggg acgactctag
    18001 caggaaaaga catcttcaaa ctatacgaca catatggctt cccggtagaa ttaacggaag
    18061 aggtggcaga agatgaagga ctgaaagttg atcatgctgg ttttgaaaaa gaaatggatg
    18121 cacaacgcga acgtgctcgc tctgcccgca gtactgaaaa atcaatgggg gtacaatctg
    18181 ctttattgac agatatcaaa gtagaaagta aattcattgg atacacagaa caagaatcag
    18241 atagtcaatt attggtcatc gttaaaggtg agtctctttt gaatgaagtc gcagaaggtg
    18301 aagctcaaat gatctttgat caaacacctt tctatgcaga aatgggtggg caagtagcgg
    18361 atcaaggatg gatcatgaat gaagtaggcg aaatcgtagc agttgttaca gatgttcaaa
    18421 aagcgcctaa tggtcaattt atgcacaccg tagaagtaaa agcaccaatg agtgaaggac
    18481 aaacctatca tctattgatc gatcaatcga tgcgcaatcg gatcatcaaa aaccatacag
    18541 caacccatct actgcacaag gcattgaaag aagtacttgg cgagcatgca aatcaagctg
    18601 gttctttagt cgctcctgga catttacgat tcgactttac ccactttggt caaatcactt
    18661 cagatgaatt agctgaaatg gaaagaatcg tcaacgaaaa aatctgggaa gctatcccag
    18721 ttgaaacaat tgaaacagat atcgatacag ccaaaaacat gggtgcaatg gctttgtttg
    18781 gtgaaaaata cggacatgac gttcgagtag taaatattgg cggctggtca atcgaactgt
    18841 gcggaggcac acatgtagca aatacagaaa acatcggaat cttcaaaatc gtttctgaat
    18901 caggaattgg tgcaggcgtg cgacgaatcg aggctgtaac aagtaaagaa gcgtacgaac
    18961 tacttgaaga agaagaaaaa caactaaaac aagtagcaac aattgttaaa tcacctcaat
    19021 tgaaagaagt tgtttcaaaa gcggaacaat tgcaacagca agtaagagag ctacaaaagg
    19081 aaaatgaaca attagctagc aagttagcga atcaacaagc tggtgatgta ttcaaagatc
    19141 cagaaacgat caatggaaaa acaatcattg cagcacaagt aaaagtgaaa gatatgaatc
    19201 agctgcgtca attagctgac caatggaaac aaaaaaatat ttcagatatt cttgttttag
    19261 caaacgaaca agaaggaaaa gtcagtctgc tagcggcaat gacgaaagaa tcaaatgaag
    19321 ctggactaaa agctggcgac ttgattaaag caatcgcacc gaaagtaggc ggtggcggtg
    19381 gtggacgtcc agacatggca caagctggcg ggaaaaatcc tgctggtatt ccagatgcac
    19441 ttaaagctgc gaaagaatgg gttgcgcaac aatagggcgt ttacgtttga cagaaaaaca
    19501 aaagttcaaa cattgaacga aacatgatta tgggggcgaa gacaaacaag tcttgccctc
    19561 atactttttt caattggatg gcgcatctaa tcaaatggtc ggattcggat ggtctttagt
    19621 tgacgaaagt gacagaaatt tttacgatta gtataaataa aaaggggtga aaaaatgaat
    19681 agtagaaata agacctttcg actagtctta cgtgcgatct tgttagcaat catcatcgta
    19741 caagcgatgg ttccttggct ggggtttatt ccattaggat tcattagttt aacaattatc
    19801 catatcacag taattattgc tgcagtagta ttaggcccaa aagatggcat ggtgattggc
    19861 ttgttttggg gaatcgctac aattgttcgt gcctatgcca tgccaacaac gccatttgat
    19921 acattagtgt tcacgaatcc gattatctcg gtagttccca gagtacttgt tggcttagta
    19981 gcaggcttgg ttttccactg gatttatcaa aaaaataaat caataacgat cagctcagcc
    20041 tttgcagggg tactgggttc attggtcaat acagtcttag tgttaggatt catgggatta
    20101 ttttatacag gtgcaacggc agatgcctat ggtgtagact ctgctctttt atttaaaacc
    20161 ttagcagggg ttgcagcaat caatgggata ccagaagcaa taggtgcggg aatcataaca
    20221 ccattactag caaaagcgtt atttgcagca acaccgttga aaccagaata atgaccatct
    20281 tttaatagta ataggacgca cgatagatag aaaaaggaag agaggagagg aaaacaatcg
    20341 tcctctccat ctttttacct gagcaacaag caaactagaa ggggtaaaaa acttggctaa
    20401 gaaacaagaa gaaaaaaaga aacaaatacc gcaacagcgg acaaaaaaag gagtactagc
    20461 gggtatctta ggattggtta cgaatctgat cttgtttgtc tctaagctaa tgatcgggct
    20521 tttttcagga agtgtctcca ttatggctga tgcagtgaac agtctatcag atactgcttc
    20581 atccatattg actttgatcg gatttaaaat tgctgcaaag ccagcagatc aggaacatcc
    20641 ttatggacac gaacgcttcg aatatatcag tgggttattt gtttcgatca tcattactta
    20701 tgtaggattt caatttttag agtcttctgc tgaaaaaatc atccatccag aaaatgtctc
    20761 tttaacaccc atcgtctttt ttgtcctaat catttccatc tttttgaaat ttgcacaagg
    20821 caaaatgtat caggttattg cgcaatccat tgattctgaa acattgagag ctacagcaac
    20881 agatagttat aatgatgtct ttactacact ggctgtattg ctttcagcag caatcgaatg
    20941 ggtaactgga tggcgtattg atggatatat aggttttgct ttagcattat atatccttta
    21001 tagtggattg acgatgcttc gaaattttgt atacgaacta ttaggaagtc gcccaacgcc
    21061 agcagagatt gaagaaatgg aagagcattt aaatgcttgc gatgatatac ttggctatca
    21121 tgatttattg gtgcataact atggacccaa taaacgcttt gcatcagtcc acatcgaagt
    21181 agatgaatca atgaatttga atgtggctca taagattatt gatctgatag aaaaagactt
    21241 taaaaagtct ttggatgtgg aacttgtctg tcacttggat ccaatgcctg ttgataacga
    21301 aaaatactat aatatcttgc ggcagttaaa gcaaattctt cttgaaacag gtacagggct
    21361 taatattcat gattttagag taattcatca aggaaaaacg ctgcaatttg atgtagtggt
    21421 ccctgagaaa ttccagtttg ttgatacaga actagaaaga atcttacgta gtcaaataga
    21481 agaaaaaatc ggagattacc aattggaaat tacgtttgat cacaattatc ttttatatta
    21541 aatgagaaac tataaaaaga ctccttaaag tttttaattt aaagagtctt tttttctttt
    21601 aattgtttat ggagagcctg acgtgcaaga ttatctgctc ctttattttt gttttcgggc
    21661 aaccattgca ggatcaataa agaaaattcg gatagaagtt cttggattct ttgaaggtag
    21721 ggaaggaaga gctcgttctt cgttgcgttt ttgtcgaccg tagaaactag tgttttgctg
    21781 tctgaaaaac agaaaatcgt ttcattctgt aagtcgtttt tctttagaaa ttctaaagtt
    21841 tttaaaaaga cttcgaactc tgcttgatgg tttgtcccta tagaaagcgg gatagatagc
    21901 tgttcttgtt taccttgata gacaatcaag attccacctc cactaggacc agggttgcct
    21961 tttgtagagg catcaatgta tacttttaac ataaaaagag attctccttt ttgttcaaac
    22021 taatgttatg gtaagattat atcattactg aatcggtgag aggttagaga atgaaaaaaa
    22081 atattcgatg gcagcctgaa ctggcacata cgatcatcta ttggtcattg accttgatca
    22141 ttctgttttt tagtctgatt ctttctttag aaaacacagg tccttattgg aaaagcaatt
    22201 ttgttttagg tatttttctt gtttttctat ttctaggttt gcggcgttct tttattttaa
    22261 aatcaaactc tgtgattata cgttatgctg ctttttggaa agaccgagaa atatctgtcg
    22321 gacagattcg atctttgacg ccaactaata ctggggtgca aatgatgttg caagatgaga
    22381 aagccccatc tgttttcatg atgcgaaaaa aagatcaagt agtccttttt gatcattttg
    22441 aaaaaaacgg acccaaatca gaatcaattc cttatcctta tacgaaaaaa agaaaaagct
    22501 aattttaaaa atcagaacct aaaaaaacga atttttacag gaaaagaaaa gagtgagacg
    22561 tacggttcat cccgtttagc gtctcactct atttgtttat ctattcatgc atactagtat
    22621 cggtagtctg aacgttgcct ttcttgacat gggcagcttg tgttcctcga ttcccttcga
    22681 tgatctcaaa ttcgaccact tgattttctt ccaaagtttt gaatccctct tcttcaatcg
    22741 cagtaaaatg tacaaaaatt tcttctgtgt catcataact gataaagccg tatccttttt
    22801 tgttgtcaaa ccattttaca attcctgtgg tcaaagcact cacgaccttt ctaaagtctg
    22861 atcatagcct tcattataaa gagtatttat aagtacgtca aataaattaa aaacctgtaa
    22921 ttttatgttt tttttctgaa taaaaaaagg tataattggc atatcttaac aaagaagata
    22981 ttttgttgaa taaagttcgt gaaaaggggt aatcaaatga agaatgatct gcaaatttca
    23041 caagaaacag agctagaacc gattgttgaa gtggctagta aattagatct taaggaagac
    23101 gatttggaac tgtatgggaa atataaggcg aaaatcaatt tttctggaat caatcgatca
    23161 aaggataacg ctgaggggca tttgatctta gttacttcca tcaatccaac accagctggc
    23221 gaaggaaagt caacgatcac tgtagggtta ggagacgcgc tcaataaaat caataaaaaa
    23281 tctgttattg cattgcgaga accctcactt ggtcctgtga tgggaatcaa aggtggtgct
    23341 gcaggcggag gatatgcaca ggtattgcct atggaagata tcaacttgca ttttactggt
    23401 gacatgcatg ctatcacaac agctaataat gcattatctg ctcttttaga taatcatatt
    23461 catcaaggaa atgaattgaa catcgatgcc cgccgagtga tttggaaacg ggtagtcgat
    23521 ctcaatgatc gtgaactgcg taaagtaatt gttggcttgg gtgggccaat ccaaggtgtt
    23581 ccaagagaag atgggtttga tatcacagtt gccagcgaaa tcatggctat cttgtgtctt
    23641 gcggcagacc ttgaagattt aaaggctcgt cttgcaagga tcgttgtcgg ctatacattt
    23701 gaccgtcagc ctgtgactgc tggagacttg aaagcagaag gcgctctggc attactgctg
    23761 aaagatgcaa tcaagccgaa tcttgtacaa acaatttacg gaacaccagc ttttgtccac
    23821 gggggaccgt ttgccaatat cgctcatggc tgcaatagta ttttagctac caagacagca
    23881 ttgagattag cagattacgt tgtcacagag gcaggatttg gtgcagatct tggcggagag
    23941 aaatttttag atatcaaagt acctaacttg aaaaaagcgc cggatgctgt ggtgattgtc
    24001 gccactgtcc gtgcattgaa aatgcatggc ggaatgaaaa aagatgaact taaaaatgag
    24061 aatcttgatg ctttgaaagt aggttttgca aatttaaagc gccatatacg taatatggaa
    24121 caatatcagc tacctgtgat cgtcgcaatc aatgaattcg taacagatac agacaatgaa
    24181 ctcacactgt tggagcactt gtgtgaaaat caggggattc ttgccaagag agcgagtgta
    24241 tgggcagatg gagcagaagg cggcgttgat ttagcaaaag ctgtagtacg attgattgat
    24301 agaaaagaag ctaattacaa gccattatac cgcctagaag aaacgataca agaaaagaca
    24361 gaaacaatcg tcaagaaaat ttacggagga aatggtgttg ttttcagcaa aaaagcgcaa
    24421 aaacaaatag aagagtttac gaagaatgga tgggacaaac tgccgatttg tatggcaaaa
    24481 acacagtatt cattttctga tgatccttca ctgctaggtg caccggaaga tttcaccatc
    24541 acgatccgcg aaatcatccc aaaattaggg gcaggatttt tagtagcctt gactggagat
    24601 gttatgacga tgcctggttt gcctaaaaaa cctgctgcat tgaatatgga cgtgacaaat
    24661 gacggagaag tgttgggatt gttttaagct tgattataat ctaccatcga aggagctgta
    24721 ttttgactac agcttctttt tttagacgat agggaattaa ttttgtttac actaggatca
    24781 ttctttttct ttattgtatt ttgagctata ctaaagcata ataggagggt atgatgaaaa
    24841 cgacatttat aagaaaaacc ggtttttacg gtatgggtag ctcgattcag ctaagaatca
    24901 acaatgaaaa acgcagtgtt ctcagtcatg aacaaaagat aacgcttgat ctggaagtgc
    24961 cctttacgat gcaggtcacc ttttattggc taaaaagccc cgtttataca gtgaaagaag
    25021 caaagcctgc ttatgtgatc acaatgaatt acttgattct tcaaatctat ccgtttttat
    25081 ttctagcaac tgggttgggg gccgttttcc tacaaaattt atttttcagt ttttttgcag
    25141 ttattgtgtt gattgcgttc tttttctata taaaaaaccg ggcatatttg atcaaggagg
    25201 aaaattatgg caaactctga tcttttttta atcagtttca atcgaatcga aaaatggatg
    25261 agagacgaga tgggaaatgc acgaaatatg ggatttaccg aacttgtcag acgcctagct
    25321 caaagaaaac aattaatgat cagaaaatac gaagatgatc ttttacagct tgctcagtta
    25381 aggaatgcga tcgtccatga tcggatcgca gtcgattttg tcattgcaga gccaaatgat
    25441 tggacagtca agcgaatcca gctgatcgaa caagaactga ttcagcctga aaccgtgctg
    25501 cctcgatttg caaaacgtgt aacaggtttt gaacaagatc ttccgttact tgaactgtta
    25561 aagatagtag cagaaaaaag gtattcacaa tttccgttat acaataaagg aaaatttgta
    25621 gcattgatta ctttacgtaa tatcgggttt tggctagcaa aagaaagcca aaaaggtccg
    25681 gttgatttaa caaataaacg agcgttggat ttgatcatcc aaaacggaaa gtacacaaat
    25741 tatcattttg ttccagctaa aacacatatt tacgaggttg aagcgatgtt tcgtgagcaa
    25801 gggactttgg aagccgtact tatcactaaa gatgggaatc ccgatggtaa tctgctcggg
    25861 atcgtacgac ctcgtgatat ttataaacaa atagaaaagg attgattttt tgttaattat
    25921 ttattggata ttcagcgctt tgctagtagg agtggatcag ttggtcaaat ggtggatcac
    25981 tgataatcta gcgcttggag agacgaaaaa tcttatacca ggtattttgt cactgaatca
    26041 tattcgaaac actggagccg catggagtat gctagaaggg aagatgtggt tctttactat
    26101 agtgacactc attgcagtcg ttgtgattct cacattgatg attaaaaatc gtgaaaaagg
    26161 taatcgctgg ttcatgattg gattgagttt gattctagct ggtgcgattg gcaattttat
    26221 cgaccgtatt cgtcttggct atgtagtaga tatgttccag acagatttta tgagctttcc
    26281 gatttttaac gtagcagatg tgacacttgt atgtggagtg atttgtatgt taatttatat
    26341 tattttagat gaaaaggatc aaagaaaaaa atgacagaac ttaaaacgac gattcaccat
    26401 gaatctggta ggatcgataa agtattgact aatttattca ccgattacag tcgttcgcaa
    26461 attcagctgt ggctgaaaaa tggagcggtt tctgtcaatg gacagccagt caaagcaaat
    26521 tacaaagtaa aacaaggaga cgagattact gtcttgatcc ccgaaccaga aacattagat
    26581 gtggtcccag aaaatatccc gttgacgatc gtttacgaag atgaagatgt agcggtgata
    26641 aacaaacctc aaggaatggt cgttcatcct tcagcaggtc atacgcatgg cacaatggtc
    26701 aatgcgctga tgtaccatat caaggatctt tctacgatca acggcgttat tcgtccaggt
    26761 attgttcacc gaatcgacaa agatacttcc ggactgctaa tggttgccaa aaatgaccag
    26821 gcacacgaat cattagctca gcaattaaaa gataagacct ctttgcgcaa atatgttgct
    26881 ttagtccatg gagagattcc tcacgaaaaa ggaaccatca atgcgccaat cggccgttcg
    26941 aaggaaaacc ggaaaatgca ggcgataaga gaagatggga agtcggctgt cacacatttt
    27001 actgtattgg aacgtttcga aggatttact ctggttgaat taaaactaga aaccggaaga
    27061 acacaccaga tccgtgtgca tatgaagtat atcggttatc cgttagctgg agatccagta
    27121 tacggaccga agaaaacttt gaaaggcaat ggtcagtttc tccatgcgca aatgttaggt
    27181 tttacgcacc cgactacaaa cgaaaagatg atctttgaag cacctcttcc agaagttttt
    27241 gaaaaaactt tagaaaaatt aagaaaaaac attgcatttt aatttagata acggtatagt
    27301 aattaacaac gaaagcataa aacttaatag aattccttta agtttagtcc cgtgaggcta
    27361 agaagggtgg gagcaaggat acgtatggtg cgccataagt agattgctca ttgacatagt
    27421 atagctatca gtgtatcagt atacgctcag tctaaacatt cctcctgtcc tcttagggca
    27481 ggagtttttt tatgcaaaaa agtaggggga atgaaaatgc aagcaaaaga agtagtagat
    27541 caggtaacca tgaagcgagc attgactcga attacttatg agattatcga gagaaaccat
    27601 agtatccaag atatcgtatt ggtaggtatc aaaacacgag gaatctacat tgcatcaaga
    27661 attgccgaac gcctgaagca gctagaagat atcgatattc ctgtaggaga actggatatc
    27721 actttatacc gagatgacaa aaaagaaaat ccagaagaac cggaacttca ttcttctgac
    27781 atcccagttt ccttagaagg aaaagaagtt attttgattg atgatgtttt gtatacagga
    27841 cgaacgattc gagcagcaat ggacgcagta atggattttg gcagaccaag aaaaatttca
    27901 ttagcagtcc tggtcgatcg tgggcatcga gaattgccaa tccgtgcaga ttatgttggg
    27961 aagaacattc caacatctcg ggcggaagag attttagtag agatgcaaga gcttgatgga
    28021 caagatcgca tcatgatttt gaaagaggaa gattagagga gagagaaaca tgtcggagaa
    28081 aaaaggattt cataatgatg acgtggtgtt agatatccac gatcgcccaa ccccaggaca
    28141 ttgggtagga ttaagtttac aacacttgtt cacgatgttc ggagcaaccg tgttagtccc
    28201 catcttagta ggtatcgatc caggtattgc gctagtcagt tcaggactag gtacattggt
    28261 gtacttattt actactaaag gaaagatacc tgcgtactta ggaagcagct tcgctttcat
    28321 tgcagcgatg cagcttttga tgaaaacaga tggttatcca gcgattgcac aaggagcagt
    28381 cacaactgga ttagtctatt tgattgttgc gctgatcatc aaaaagatcg gttcagcatg
    28441 gctggataaa atcttgcctc caatcgtcgt cggtccagtc atcatggtta tcgggttggg
    28501 acttgcggca aatgcagcaa caaatgctat gtataatcag ggcacgtatg atttcaagta
    28561 tattgctgta gcgctgatca cattaggatt aacaattttc ttcaacatgt ggctaagagg
    28621 ttttctcgga ttgattccga ttttactagg tattattagc ggatacatcg tagccttgct
    28681 gtttggaatc gtggatacta agccaatcgt agacgctgcc tggtttgctg ttcctaactt
    28741 tgagattccc tttgtacaat atgaaccaaa gctatatctt ggagcaatca caacaatggc
    28801 acccatcgct tttgtgacga tgacagagca tatcggccat cttatggtat tgaataaact
    28861 aactaaacgt aatttctttg aagaaccggg tcttcataag acgttgacag gtgatggatt
    28921 agcacaaatc gtggcagcct ttgtcggagg tcctcctgta acaagttacg gtgaaaacat
    28981 tggtgttctg gctatcacac gtgtccatag cgtgtttgtg atcggtggag ctgcagtatt
    29041 tgcagtcatt ttaggattcg taggaaaatt aagcgctttg attttaagta tccctggacc
    29101 agtcatctca ggaatcagtt ttatcttatt tggagtaatt gccgcaagtg gaatgaagat
    29161 tttaatcgag aacaaaatcg attttgataa aaagaaaaat ctgttgattg cttcagttat
    29221 cttagtagta ggaattggcg gactcgtctt tgaagtaggc acatttacat tgtcggcaat
    29281 ggcattagca actgtacttg gcatcatttt aaatcttatc ttaccagaaa cttcacgaag
    29341 cgaagaaaac taaggaggaa acattatgat catcaaatca gaacgtatca gcttaaagca
    29401 tttattgacc gttgaggcac ttactgatca agaagttatg ggcttgatcc gcagagggca
    29461 ggaattcaag caaggtgcta attggactcc gcaaaagcgc cagtactttg ctacaaacct
    29521 ctttttcgaa aatagtactc gcacacataa aagcttcgat gtagcggaga aaaaactagg
    29581 gctagaagtg attgaatttg aagcaagcac aagctctgtc caaaaagggg aaacactata
    29641 cgatacagta ctgacgatgt cagcattagg cgtagacgta gcggttatcc gtcatggtga
    29701 tgagaactat tatgacgaac tgatacaaag taaaacaatc cagtgttcga ttatcaatgg
    29761 aggagacgga agcggacagc atccaaccca atgcttgctt gatttgatga ccatttatga
    29821 agaatttggt cattttgaag gtctgaaagt tgccatcgta ggtgatatca ctcactctcg
    29881 cgtagctaaa tccaatatgc agatgttaaa acgtctaggc gcacaagtat tcttctcagg
    29941 tcctagagaa tggtacgacg atgaattcga agtgtacgga cattatatgc cattggatga
    30001 actactagat caagttgatg tcatgatgat gcttcgggtg caacacgaac gccatgatgg
    30061 aaaagaaagt ttctcaaaag aaggctacca tcaggaacat gggttaacag ttgagcgtgc
    30121 gaagaagttg caaaaacatg caatcattat gcacccagca ccagttaacc gtgatgtaga
    30181 attagctgat tcattagtgg aaggattaca gtcgcggatc gttcagcaaa tgagcaatgg
    30241 agtattcgtc agaatggcaa tcttagaagc agtgctaaat ggaaaagcgt aattatagga
    30301 ggatgaaaaa atgaaaacac tcattaaaaa cggtcagatc aatacacgaa aaaatatgac
    30361 gacaccagca gaaatttgga tagaagatgg gcgaatcaaa gcaatcggta caggattctc
    30421 agaagccgaa tttgatgaag tgtttgatgc aaagggacaa ttgatcacac ctggtttagt
    30481 ggacgtccat gttcatttga gagagccagg atttacatac aaggagacga tcgaagctgg
    30541 gactcgctca gctgctcgtg gcggtttcac tactgtatgc gcgatgccga atttaaatcc
    30601 tgtacctgat acggcagaaa agctgagaca agtatatgac atcattagaa aagatgcagt
    30661 tgtgaaggta ttgcagtatg cgccaatcac agaaaatctg cgaagtgaga aattagttga
    30721 tcaagaagca ctgattgaag aaggtgcttt tgctttcaca aatgacggtg taggtgtcca
    30781 aacagctggg acgatgtatc tggctatgaa agaagctgca aaaaacaata aagccctagt
    30841 agcccataca gaagatgaat ctttattatt tggcggcgtt atgcacgctg gcaagaaggc
    30901 agaagaactt ggtcttcccg ggatcttaag cgtaaccgag tcttctcaaa ttgctagaga
    30961 tctgttgcta gctgaagcaa ctggtgttca ttaccatgtt tgccatgttt ctactaaaga
    31021 aagtgtccgt gtaatcagag acgcaaaaaa agcaggaatc cacgtaacag cagaagtttc
    31081 accccatcat cttattttga tcgacgaaga tattcccgaa gatttcggct tttggaaaat
    31141 gaacccgccg ctaagaggac gggaggacag agaagctttg atcgaaggct tgcttgacgg
    31201 tacgatcgat tgcatcgcta cagaccatgc cccccatgga ttagaagaaa aaagccaaag
    31261 tttcatgaaa tcaccatttg gtatcgtagg aagcgaaaca gcgtttcaac tgatctatac
    31321 tcattttgta gaaactggtc gattcacgtt ggaacaagtg atcaattggt tggcagttaa
    31381 accggctgag atttttggtt tgaatgcagg aacgctaacg gttggtgccc ccgctgatgt
    31441 agcagttttt gatattacac aaacttgcac gatcgataaa gaagactttt tatcaaaagg
    31501 ggagaataca ccttttatcg gctggaaagt aaaaggcgaa acacaaatga cttttgtcaa
    31561 cggaaaactt gtgtggcaga agggagaata aaagtatgga acgtttgctt atattagaag
    31621 atggaacagt tttcgaagga aaggcttttg gtgcagatgc tagtgtcttc ggagaaatcg
    31681 tttttacaac aagtatgact ggttatcaag aaactatcac tgatcaaagt tttaacggtc
    31741 aaatcattac atttacctat cctatggtgg gtaattatgg ggtaaataga gatgattatg
    31801 aatcgatttc tccaacatgc aaaggagtag tagtaaaaga acatgcacgt gtagcttcaa
    31861 actggcgaaa ccaaatgacg ctagattcat ttttgaaaca aaaaggaatt cctggaatat
    31921 caggtatcga tacgcgcgcc cttactcgca aaatccgtga agtcggaacg atgaaagcta
    31981 gtatagttga tgctggagat tccttcgaac atgcatttga tcagctgaaa gcaaccgttc
    32041 tagcaaccaa tcaagtgcag caagtctcaa ctaacaaacc ttatccaagt ccaggaacag
    32101 gtcggaaagt cattgtcatt gacttcggct tgaaacatag tattttacga gaattgagta
    32161 aaagaaactg taacctcaca gtacttccct ataacacaga tgccgagacg attttagcgc
    32221 tatcaccaga cggagtcatg ctgacgaatg gacctggtga tccaaaagat gtgccagaag
    32281 caatcgaaat gatcaaaaaa atccaaggaa aagtaccgat ttttggtatt tgtctcggtc
    32341 accagttatt tgctttagcc aacggtgcag atacctataa aatgaaattc ggacatcgag
    32401 gattaaatca cccggtacga gaagtagcga ccggaagaat tgattttact tctcaaaatc
    32461 atggctatgc agtcgatgaa gccacgattg atccagaaaa actcatcgta actcatgtag
    32521 aagtaaatga cggaacagta gaaggagtca aacacagaga ttatcccgct ttcagtgtgc
    32581 agtttcatcc agatgctgcc cctggtccgc atgatgcact acatttattt gatgaattta
    32641 tggaaatgat ggacgcagga aaggagcagt aaacagatgc cgaaacgtac agatatcaag
    32701 aaaattatgg tgattggatc tggtccgatc atcattggtc aagccgcaga atttgactac
    32761 gcaggaacac aagcctgcct tgccttaaaa gaagaaggat atgaagtggt tttagtaaat
    32821 tctaatcctg cgacgatcat gacggataaa gaagtggcag atcaagtata tatcgaaccg
    32881 atcacacttg aatttgtatc aaggattctt cgaaaagagc gtccagatgc tctccttcca
    32941 actttaggcg gacagacagg cctcaatatg gcaatggagc tggcagcatc aggtatttta
    33001 gaagaattga acatagaact tctaggaaca aagctttctg cgatcgatca agcagaagat
    33061 cgtgatctat tcaaacagtt gatgaaagaa ttgaatcagc caatccccga gtctgaaatc
    33121 gtcaatacag tagaagaagc tgtagatttt gcaaataaaa tcggctatcc tgtcatcgtt
    33181 cgtccagcct ttactttagg cggaactggt ggaggtatgt gtgaaaatga agaagaatta
    33241 cgtatcgtat cagaaaatgg attaagtctt tcccccgcta cccaatgttt gattgaacga
    33301 agtattgcag gttttaaaga aattgaatat gaagtgatga gagattcggc tgataatgca
    33361 atcgttgtct gcaatatgga aaattttgac ccggtgggta tccatacagg tgattctatc
    33421 gtatttgccc ctagtcaaac attgtctgac catgaatatc aaatgcttcg tgatgcttct
    33481 ctttcgatta ttcgagcact taagatcgag ggaggctgta atgtccaact cgctcttgat
    33541 ccacatagtt ttaattatta tgtgattgaa gtcaatccgc gtgtttctcg ttcatctgcg
    33601 ttagcaagta aggcgactgg ctatccgatc gctaagctag ctgctaaaat agcgcttgga
    33661 ttaacattag acgaaatgaa aaatcctgtt acaggaacga cttttgcaga atttgaacct
    33721 gcattagatt atgttgtagc taaaattcct cgctggccgt ttgataagtt tgaaaacgga
    33781 gaacggcttt taggcacgca gatgaaagca actggtgaag tgatggctat cggtcggaat
    33841 atcgaagagt ctttattgaa agctgtgcgt tctttagaaa tcggtacaca ccatatcgaa
    33901 cttcctgagt tggcagacat cacggacgat gacctaatcc aaaaagtggt tcgagcacaa
    33961 gatgatcgcc tgttttatgt agctgaagct attagaagag ggtacaccat agaagaatta
    34021 gcggatttga caaaaatcga tttattcttt ttagataaac tgttgcatat cgtagaaatc
    34081 gaacagcagt tgaaacagca gccgttggat gacaagatat tgaaaacagc gaaacaaaac
    34141 ggttttacag acaaaaaagt ggcggagctg tgggaaataa acgaagaagc cgttcgccag
    34201 atgagaaaag aaaatgattt gattcctgta tataagatgg ttgacacttg tgcagcggaa
    34261 ttcgaatcaa aaacacctta tttttatagt tcctatgaat atgaaaatga aagttcacga
    34321 tcagaaaagc cctcaattct agtactggga tctggtccta tccgtatcgg acaaggggtt
    34381 gaatttgact atgcgactgt gcattctgtc aaagcaatcc agcaggcagg atacgaagca
    34441 atcatcatga acagcaatcc tgaaacagtt tcgactgact tttctatttc ggataaatta
    34501 tattttgagc cattgacctt cgaagatgtg atgaatgtga tcgagctaga acagccgata
    34561 ggagtaatcg ttcaatttgg tggtcagaca gcaatcaact tagccgaacc tttgactgct
    34621 gctggtgtaa aaatcttagg gacaacgatc gaagacttgg atcgtgcgga aaatcgtgat
    34681 ttattcgagc aagcgctgaa agagttggat attcctcaac ctttagggga tactgctaca
    34741 agtaaagaag aagccgtggt tatcgcttcg agaattggtt atccagttct tgttcgtcca
    34801 agctatgtat taggcggacg tgcgatggaa atcgttgaaa accagcgaga tttagaagat
    34861 tacatggaac atgctgtgaa agcatctcca gaacatccag tactagtgga ccgttacttg
    34921 cttggtggtg aatgcgaagt ggatgcgatt tgtgacggtg aaacagtact catcccaggg
    34981 atcatggagc atattgagcg tgctggtgtc cattcagggg attcaatggc tgtgtatcca
    35041 cctcaaacat tatctgaatc gatcaagaaa acgatcgaag attacacgat ccgcttagca
    35101 ttaggtttga attgtgtcgg gatgatgaat atccagtttg tcatccatga aaatgaggtt
    35161 tatgtgatcg aagtcaatcc tcgagcaagt cggactgttc cattcttaag taaggtgaca
    35221 ggaattccaa tggcacaaat tgcgacgaaa gctattttgg gagagaaact aaagacactt
    35281 ggttatgaaa acggacttta tccagaaaca aaagaagttc atgttaaagc acctgtcttt
    35341 tcttttacga aattgcagaa agttgatact tacttagggc ctgaaatgaa atcgactgga
    35401 gaagtaatgg gatctgacag acaattagac aaagctttgt acaaggcatt cgaagcttct
    35461 ggattacact tgcctgaata tggagccgta ttatttacga ttgcggacga aacaaaagaa
    35521 gaagcactag aattagctaa acgttttaca gaaattggct atagtctgat aggaacgaaa
    35581 catacagctg cttatttcga aaaaaatggt ctggtcgttg aaacagtagc gaaaatttct
    35641 gaggaagcag cagaaaaaaa tgtagtcgac ttaataagag atggtaaaac acaagtggtt
    35701 gtcaatacga ttgataaaga tcggggaaat gcttctaaag acggtttcat catccgaaga
    35761 gaagcagttg aacatggtat tccattgttt acttctttag acacagcaga tgcgatcgtt
    35821 cgagtgatgg aatcccgtgc tttttcaact aaccctattt aacggcgagc acatgttaag
    35881 acaaaaagac gaaaaaccgt tgcaggaggg gacagatgaa acaagaaatg atggaaatcg
    35941 ttcagcaaaa acagctggca ccaaagattt ttgaaatgac attgaaaggt gatctagtaa
    36001 aagaaatgca gcaaccagga cagttcttgc atattctcgt gccgcgtgca gacttgctat
    36061 tacgccgacc gattagcttg aatcatatcg accacgaaac aaatacttgc cgtatcattt
    36121 atcgggtgga aggtgagggg actagagtct tctctactct ttctgccgga gaccatttag
    36181 atgtcatggg tcctttaggc aatggttttg atttgtccaa tcttcataca ggagacgaag
    36241 tatttataat cggtggagga atcggtgtcc ctcctctata tgagttatca aaacagttag
    36301 tcgaaaaagg aattaagcca acgcattttt taggttttgc cacacatgaa gtcgtttatt
    36361 atgaaaaaga atttaatcgt ctaggagaaa caagaatcgc tactgacgat ggaacatacg
    36421 gtatgcacgg aaatgtcggt aatttattgt taggaacgaa gacggaacct acagctgttt
    36481 ttgcatgtgg ttcaaacggg ttattgaaga cagtggaaca gctgttttct tcgcatcaaa
    36541 atgtccagtt atcattagaa tcccgcatgg catgtgggat aggggcttgc tatgcttgcg
    36601 tttgtcatac gccggaagat gaaacaggaa caaaaagtgt gaaagtttgt gacgaaggac
    36661 cgatttttaa aataggagag gtaattatat gaatgagaat cctttagcgg tcaaacttcc
    36721 tggcctagaa ttaaaaaatc ctatcatgcc ggcaagcggc tgtttcggtt ttggcaaaga
    36781 atacggaaaa tactatgatt tgaatcagct tggatcgatc atggtcaagg caacgactcc
    36841 aaatgcccgc tttggcaatc ccacgccaag agtggcggaa acaccatctg gcatgttgaa
    36901 tgcaattggt ttgcaaaatc ctggtctaga cgttgttatg cataaacttc ttccagaatt
    36961 agaggagtac aatgagttac ccatcatcgc aaatgttgct ggtgcttgtg aagaagacta
    37021 tgtagaagtt tgtagcaaaa ttggcgaagc accaaatgtg aaagcaatcg agctgaacat
    37081 ttcttgccca aatgtcaaac atggcggaat tgcttttgga acagattctg atgttgcttt
    37141 tcaattaaca caagcagtaa agaaagtttc ttctgttcct gtctatgtca aattgtcccc
    37201 aaatgtaaca gacatcgttc caatcgctca ggcaatcgaa gctggagggg cagatgggtt
    37261 tacgatgatc aatactcttc ttggtatgcg gattgacctt aagacaagaa aaccgatttt
    37321 agccaatcaa acaggtggac tctccggacc agcaatcaag cctgtcgcta ttcgtttgat
    37381 tcaccaagta gcaagtatat ccagccttcc aatcattggg atgggcggcg tgcagacagt
    37441 ggatgatgtt cttgagatgt ttatggctgg agcaagtgct gtagctatag ggaccgcgaa
    37501 tttcaccgat ccgtatatct gtcctaaact aattaaagag ctccctgtgc gaatgagcga
    37561 actaggcatc gaatcgctgg aaagattaat caaagaagtg agagaggaac gtgaacgatg
    37621 aatcaactag accaacgacc aattattgca ttagattttt caactaggca agaggtagaa
    37681 gactttcttc gatttttccc gaaagaagaa aaattgtttg tgaaaatcgg aatggaattg
    37741 ttctaccaag aaggacctga aatcgtacgt tatctaaaag aggctggtca taatgtattt
    37801 ttagatctga aacttcacga tattccgaat acggtcgaaa aagcaatgcg gggattggca
    37861 aagcttggtg ttgatctcac ctgtgttcat gccgctggcg gtattcgtat gatggaggcg
    37921 gcaatgcgag gattagaaga ggggactcca gaaggtggaa aacgtccgct acttctagct
    37981 atcactcagc ttacttctac aagtgaagaa gaaatgcatg ccgatcaact aatcgaagtt
    38041 tctttagaaa aaagtgtgat ccattatgct agctgtgcca aaaaagcagg attagatggt
    38101 gtggtttctt ctgcttggga agtagaagcg atcaaagaaa tggcaggaga cgaatttgtt
    38161 tgcttaactc cgggtataag accagaagga acagtcgcag gagatcagac aagagtagtc
    38221 acaccttctc aggcaaaaaa gatcggctca acttttatcg ttataggtcg accaatcact
    38281 cagtcaacta atccttataa agcatatcaa acgattcaaa cagaatggag ctaacctaaa
    38341 atgaatgttg aacaaagtat cgcaaaagac ttattagaaa ttgaagcagt atttttaaat
    38401 ccaagtaatc cttttacatg ggcaagtgga atcaaaagcc caatttactg tgataaccga
    38461 atcacgatga gctatccaaa agtccgaaaa gagatcgcaa aaggattagc aagcaaaatc
    38521 aaagaagctt accctgaagt acaagtgatt gctggaactg caacagcagg aatccctcat
    38581 gccgcatggg tagcggaaat actggatttg ccaatggtct atattcgaag taaggcgaaa
    38641 gatcatggga aaggtaacca aatcgaagga cgtatatttg aaggtcagaa gatggttgtg
    38701 atcgaggatt tgatttctac aggcggaagt gtcttggaag cagctgaagc agcaaaaaga
    38761 gaaggtgcgg atatccttgg tgttgcggct atctttacgt atgagttgcc aaaaggaaaa
    38821 gcaaatttcg aaaaagccga aatccctctt atgacgttga ccaattattc tgtattgatt
    38881 gaagcagctt tagaagatcg ttatatcgat gagcaagaat taactctgct aaaagaatgg
    38941 aaaaaagatc ctgaaaattg gcagatttaa gcgcgcttga tgaagataga gaaatccgat
    39001 ttgtgtctcc agaatcatca attggaatag caaaaatcca aaaactgatt gcgagaagca
    39061 tcttttcatc aggtatgaaa agaaacttct atcgtgatca gtttttgatt tttttaaaat
    39121 tcatttttct aagactggtt agcagaaaga agagaataat ctcttctctt ttttctatta
    39181 tatatagatt ttctctattt tttggtaagg gagctgtttc aaaaatggta aagagcggaa
    39241 atgttgcgaa tatcagttat ttaatgatat gatattcgga aaaagaaatc tcaaagggag
    39301 gtaactttta tggggttgaa tgaatttatt ttaggattga acaatttaga gacgatcaca
    39361 agggcacctg gattttttaa atatacagag catacagttg cagcacattc gtatcgtgta
    39421 gcatctattg cgcaagttct aggagacatt gaagaagcga atggacaacc gatcgattgg
    39481 aaactgctct atgaaaaatc attgaatcat gactacacag agcgttttat tggtgatatc
    39541 aaaacgccgg tgaaatacgc aagcaaagag ttaagaggga tgctgcaaac agttgaagaa
    39601 aagatgacgg acgaattcat caaccaagag attccagctg agttccaaga agtttatcgc
    39661 cgtcggttgt tcgaaggaaa agatgaaacc gtagaaggag aactattagc catttcagat
    39721 aaggttgacc ttctttatga atcttttgat gaaatcatca aaaataaccc ggaaaaagtt
    39781 tataaggaga tgttcattga ggcagtgact acgattaaaa gctttgaaca tcggccttct
    39841 gttcagtatt tctttaaaga gatatttccg gaactattga accaggaatt ttatggaaaa
    39901 gaggcgtttt tagaaaccgt ctcaacgatt gtaaagaaag aagaaaaata aaaatgatat
    39961 gagatgcagg gggaagtata gttgaaaaag aatatggatg tcgaatgggg ctattctgga
    40021 gacaaaggtc cggaaaattg ggcgagttta tgtgattggt ttgcgagagg tgcggaattt
    40081 ccattgcagt cgcctattca tttgaccaag ggggaagaaa ccaaaataga aggagacacg
    40141 ctttcctttc attatcaaaa gcaacggttt actgacaagg aattcaaaaa cacgattcat
    40201 ttagtacctt ttgaccaact cagttatgta gagttcaaga aagaacgcta ttatttgaca
    40261 gatattcatt tccacttgcc ttctgaacat atcatcaacg gaaaacagga agatattgaa
    40321 tttcattttg ttcatatgaa cagcaaaaaa gagaatctag ttgtgggtgt catgtaccag
    40381 ctaatggtac aggaaggttg gctgtataat caagaaaatg gggaaagttg gaacttagaa
    40441 aaacatgagc attgggtaaa tccggatgtg ttctttccgg agcaaaaaag ccatttccat
    40501 tatattggct cattgacaac tccgcctacc agcgggccaa tccaatggtt tgtaatggat
    40561 catgtaggaa aactgaacca agagtttctt ctccccttcg atggtcagta tgctcgaccg
    40621 aacaaccgac caatccagcc cttgaatgga agagaaattt attatttcga ggagtttgag
    40681 caatgaatat tcagcagatg aaatacgtgg tcgctgtagc aaacaatggt agtttcagag
    40741 aagcggcaaa gaaaatgttt gtctcccagc caagtctttc agctggtatc aaagaattag
    40801 aaaccgagtt agggatcacg ctgtttactc gaacgaatcg aggggcatat ttaacagaag
    40861 aagggcaaga cttcttaaat cgtgccgaga aaatattggt ccagcttgat acattagaaa
    40921 ctcactatct aacgagtgaa aagagagaca atttttcgat cgctgcccag cattatgatt
    40981 tcttaggtcc tttgaccgca cagctactcg aggaattcaa agacgagatc aaacaatttc
    41041 ggatcatgga gacgacaaca gcgagagtga tcgaagaagt caaagagcag cacagtgaga
    41101 tcggcattat ttatatgaat gagcataatc aagctggtat cagaagatat ttacaacaag
    41161 gagagctaag tggccgcagc attggaaaat tcaaaacgca tattttttta aggcaaggac
    41221 atccattagc tgaacacgca gagatcacca aagaagaatt gttatcttat cctcaagttc
    41281 gttttacaca ggatggaaat aatttcccgt atttttatga agaccttatc gagatccctg
    41341 atcaagaaac agtgatttat acgagtgatc gaggtacttt gatgaatatc gttcttgaaa
    41401 ctgatgctta cgcttcagga tctggtattg tgattggagg gattcgcgag cacttgcggt
    41461 taatcccttt ggcagaaggc gaactaaatg aattttatat catttattcg gcgaaacgtc
    41521 cgctcagtga aatcgctcaa agatttatca aagagcttac tcggttactg gaccagcaga
    41581 gctaagaaaa gcagatgaga acgctgttta ttgaacaccg ttgatcaaaa aagaggggtt
    41641 gtgacagaat tttgtcacaa ctcctctttt tgtagcaaac aatttataac taattgctgt
    41701 tgtgggaaag tacgcaaata tgattctaga aacactcatt gattacctgt tttgtttcat
    41761 agctgtaatt ttttcttctt ctggtgtcac gataatcgtc ttttggtttt cataaataac
    41821 ataaccaggc ttggcgccat ttggttttcg gatatgcttt acctgtacaa gatcaaccgg
    41881 cacttgggca gagtatcggt attttgaaaa ataggcagct aactcggcag cttctgtaat
    41941 tgtttcatct gagggcttgt cacttttaat gatgacgtgc gaaccgggta tattttttgc
    42001 atgaagccaa tagtcggttt ttttagctgt cttcattgtc agctggtcat tttgaagatt
    42061 gtttttacca acaagaatga gcgttccgtc agaagaaaag tattgatcag gctgagatgg
    42121 tttcttacgt ttttgttttt ttaagttctt ctttttcagg tatccttctg aagtcaattc
    42181 ttctttaatt gcttcgatat ccattgggcc ggcaatttcc agttgagata agacagattc
    42241 aagatattgg atttcatctt tggcttcttg gatttgttcg ccaatcagct taacagcgtt
    42301 cttcagcttt tggtatcgat ggaaatattt ctgtgcattt tgatttggtg tcaatgccgg
    42361 atctagtgct attttgattg gccgatcttc ttcataataa ttaggtaaaa caacttcttt
    42421 tgcgccgcgt ggtacttgtg tcagaaaagt agtaagtagt tcgccatttc tacggtaatt
    42481 ctcagcgttt tcggattctt tgagtgtttg ttcacgtttt ttcaatttat ttttatttcg
    42541 ttttagttca ttttcgattt ttcgaatcag ttctccgcct tgttgttttg cccgatcttt
    42601 ctctgctttt tcgtgataat agctatctaa taattgactg agtgttggat aagctgttac
    42661 agcagatgcc tgttcggaca atacctgata agcaatcggt gtgaaaaaat ctttattttc
    42721 cgtttcgtaa aaagtaggaa taggctgatg gctgatggct gagaaaaatt cgtcccatac
    42781 aaccatcttt tcattaggac gttccagtaa tcttttggtc agctcttgag cgatatcgaa
    42841 accgattccc tgaaactgac gttggattgc ttttggatct agttcagttt gggaaagccg
    42901 ctgaaagatt ttttctttct cggagctaaa tgggttcaag acctcttgtt ttggcggagc
    42961 aacatattct acaccaggaa gcaaagaacg ataagtgttt tgagagcttc caatgtgttt
    43021 gatcgcatcc agtatttttc cagtttctcg attaactaag acgatcgtac tatgacgtcc
    43081 cattaattca acgatcaata tgatattttg tagatcaccc aactcgtttc gtttagcaaa
    43141 gtggaaatgg atcactcgat cattttcgat ttgttcgatg tcttctaaaa tagcgccatc
    43201 caaatatttt cgaagcatca tcacgaagtt aggcggatta tcaggattct gataatcgat
    43261 ttgggtaatc tgtacacgag cataacttgg gtgcgcagat aataataatc gatggttttt
    43321 gccgcgtgaa cgaatcacca gcacgatttc attttcataa ggctgatgga ttttcgagat
    43381 ccggccagat agtaacgttt cacgcaattc gttgaccatc gcgtgggtaa atacaccatc
    43441 aaaggacatg gttcattcct catttctttt cataagtata ttcattataa cgaaaaaaag
    43501 aagaaaaacc aatttggccg aatagtaaag caaaagtcta gaaaaatctt ggactaatta
    43561 cataaaaaac agtgtttcta tcgttgaaat ccaaattttt aggtataata aaaaatagga
    43621 gaaaaagtag aaaagaaggt gtttgttatt tgggaagtag ttaaatcgag agtacaagaa
    43681 atgaatgaac atttctcagt gtcattcgca gaaaatccaa gtaagtattt tcgacgtctt
    43741 gtttttctgc tcatcttatc attgctgtta acagttgcaa tgtttttatt gccggaaaaa
    43801 atccgagaag agcaagtaaa tagtctcgtt tattcgtcca taccaaccag caaattgaaa
    43861 ccttttgatt acagcaaagc tgatcagaaa atcaaacaaa gccgggcgat atcggtgatg
    43921 ttcagcaaac cgaacgggaa aacctatcag gacatgcttg atgtttttaa tgatcctgag
    43981 cttatggaag aattgaatcg accgatcttt tattatccga tcgtttatga tgtacatgaa
    44041 ctggaacaaa aatataacat acgaacggat gaagtgacat ttattttctt tgatggagga
    44101 aaagaagcaa atcggataac tgcgggtaag ggaggaataa ctgattttga taaggagttg
    44161 atccctgaac tgaatcgact tccgttagcc aatatcaaac aattagaaga agaattggat
    44221 agaacgggag taacttccca aaattccgag attgctaatt gagacattgg cgcgatgaaa
    44281 cggtgggtct atatagcgct atttttaatt atcagaacaa gtgatgtttg atcttcaaaa
    44341 taacaaataa aaaattaaaa aatatagttg acgaaattca aaaattcgat tagacttaaa
    44401 tcaattctta aggaaagggt gacctgtaat gaacttatca aaacgtattt cagcagaaaa
    44461 caaaaaaaat tggcgcagct ggcgtagata agttgtatta atggtgagac tttcccatag
    44521 atacaacttt ttagagtgca tttctaaagc ttgtatctat gctagacatg aacattgact
    44581 gccgcataga ttagaatcta tgcggttttt tgtgtaaaga caatgaccgc tttggccatt
    44641 gtcttttttt gtagactgga tacttgcctg attataataa ataaaaagat tgactttgca
    44701 ggaggaaaaa aatgaaaaac aaacgattaa taacagttgt agtaattatt ttagtttatt
    44761 tagcaggtgc atttatttat ggaaaagcct caggcgaaaa agaaagtact tcgacgaacc
    44821 agacaaaaaa aacaacagtt gttggggtat tgcaatacgt gagccatccg gcattagatg
    44881 agatttatcg aggaatcaaa gatggtctga aagaagaagg attaaaagaa ggagaaaatc
    44941 ttgagatcaa atttcaaaat ggacaagccg atcagagcaa gctagccaca atgagtcaac
    45001 agctagtaca atctgatcca gatgccttga ttggaatcgc aacacctgcc gctcaatcat
    45061 tagcaaacac gacttcatcg atcccagttg tgttaggtgc tgtaacggat cctgtggggg
    45121 ctggattagt cgcttcttta gagaaacctg gtggtaatgt aacaggtgtt tctgatcagc
    45181 cgcctgttac ttcacaaatc aagcttgctt ctgatttgtt gccaaatgca aaaaaagtcg
    45241 gaatcttgta tgcttcttca gaggataatt caaaatacca agtagcacaa gcagaagaag
    45301 ctgcaaaaaa agaagggtta gaatcagtaa aatatgcggt gccttccact aatgagattg
    45361 cacaaacggt tcaagtgatg agccgacaag tcgattttat ctatgttcca ttagataata
    45421 ccattgccaa tgcgatgcaa acagtggtaa aagaagcaaa caaagcaaat atccccgtca
    45481 ttccatctgt ggatacaatg gtggaacaag gtggactagc aacaatcggg atcaatcaat
    45541 atcaattagg gcttcaaagc ggtaagatgg cggcagaact tgcgagtgga aaagaaaaac
    45601 cggaaacgac gcctgtttat acatttgatc aaggggatac agtgatcaat caatctcaag
    45661 ctgatcgctt aggaatcatt gttccccaat cgatgaagga aaaagccaaa atcattacag
    45721 atgactcgca gcaagaaaca tctaaggagg atgacaaatg atcgtatcag ctatttcaca
    45781 aggcatgctt tgggcaatat taggattagg gattttcatg acttatcgga tcttagactt
    45841 tcctgatatg acgacagaag ggtccttccc attaggtgct gctgtctgtg tgacagctat
    45901 tactcatggg gtagcgccga ttgtagctac attattaggc gtaggtgctg gcatgatagc
    45961 aggactcgtt acggggttac tttatacaaa aggaaagata ccggttattt tagcagggat
    46021 tttagtgatg tcaggattga actcagttat tttgtttgtg atgcgaacac caaatctttc
    46081 tttattgaat catccgattc tacaagacag cttccatcgc tttgggttac ctgattactt
    46141 tgacacagtc cttcttggct tcatctcttt gggcatcttg attgctgtac tgctattctt
    46201 cttcaataca gatttgggtc aaggctatat tgcgacagga gacaacgaaa cgatggctcg
    46261 gtcgttaggg ataaaaaccg atcgtatgaa aattttaggt ttaactttgt cgaacggtgt
    46321 cattgcatta tccggtgcgc tgattgccca gaacgacgga tatgcagatg taaataaagg
    46381 gattggcgta atcgtcatcg gtcttgcttc catcatcatc ggtgaagtga tcttccaaga
    46441 attgacgcta gcagaaagat tgattgctat tgttgtcggc agtatcattt atcagctatt
    46501 gattttagta gtaatcaaac tagggtttga cacgacttat ctgaagttgt tctccgctat
    46561 catcttagct atttgtctga tgatcccgca attgaagaaa gcactgaact taaaaacggg
    46621 ttttgagaag gaggcatagt aatgactgat atattgacaa tcaaaaatgg cagaaaagtc
    46681 gtaaacaatg gaatgaatga agagaaagta ctattggatg aaattaacct tacaatcaga
    46741 gaaggggact tcatcactgt ccttggcggg aatggtgctg gaaaaagcac actgttcaat
    46801 acgatcgctg gtacgcttca actcagtcaa ggatcgatct cttttaaaga tcagctgatc
    46861 acaaaagatt cagaagaaaa aagagcacga ttcctttctc gagttttcca agaccccaag
    46921 atgggaacgg ctcctcggat gacagtagca gaaaatttgc tacttgcaca aaaaagaggt
    46981 caaaaacgga aattccgttt acgtaacttg aaaaatcaaa aagaagattt tttcagacta
    47041 tgtcaagaag tcggtaatgg cctagagcaa cacttggata ctccagcggg tgaattatct
    47101 ggtggtcaac gccaagcatt aagcttgctg atggcgacga tcacagctcc tgagttacta
    47161 ttattagatg aacatactgc agctttagat cctaaaacag ctaaagcatt aatgcgatta
    47221 acaaatcagc ggatcaatga acaaaaattg acttgtctca tgatcacgca tcgaatggaa
    47281 gacgccttga aatacggaaa tcgtctgatc gtactccaaa aagggaaaat cgttcaagat
    47341 atttctgaaa aagaaaagca acatttgaca atgggcgacc ttttgcagtt ctttgaagaa
    47401 gtagtggatt gatttcgaca gaaaaattcc tttcagtaga aggacttgaa aactcattga
    47461 catcttcctt tgattcatcc tatacttaaa acaataaaca attaaacata tttgggtgtt
    47521 ttgtaggatc acgtagtcaa tgattttaac aattaaatag aatattcttt gctctgacaa
    47581 gtaaacgatg agaaaagaat ttgctacttt gccacctaga cgtttcgtac tttttcgtat
    47641 caaacgctaa gtggcttttt ttattgaagt aagaacaaag gaggaataag atgatttctg
    47701 gacaaaccaa attagctggg ctctttgctg atccggcttc tcacagttta tccccattga
    47761 tgcacaatac tgcttttgaa gcaaacaaca tcgatgcggt gtatttagct ttccaagtaa
    47821 atccaatgaa tttgaagcaa gcagtcgaaa gtattcgaac ttttgatatg ttaggtgtaa
    47881 atctttcgat gccgaataaa acagcggtta ttccttatct ggatgaggta agccaggaag
    47941 cgcaattgat cggagcagtg aatacgatcg tgcacgaaaa gggtcgtttg attggctata
    48001 atactgatgg tgcgggcttc atgcaatccg tgcaagaagc tgggatttcg atcaaaaaga
    48061 aaaaaatgac tctactggga gctggaggtg cagcaaaggc agtcactgtt caagcagcgt
    48121 tagacggcgc tgaggaaatc gtagtttata aacgaaacaa ccaaacattc gctcaagtaa
    48181 aagaaacatt cgaacaactc agctatcgaa cttcttgtaa gataaaagtt cgtgactttg
    48241 ccgatacgca tcaattaaga aaagacttga aagaaagctg tcttctagtc aatgcaacag
    48301 atatcgggat gggaaagaaa atcaatcagt caccgctctt agatataagc atgatgcatg
    48361 agaatctagc ggtttttgat ttgatttatg cacctagaga aacacgttta ttaaaagaag
    48421 caaaaaaagt aggagcaaaa ggatacaatg gacttggcat gttgatctac caaggagcaa
    48481 tcgcgtttga attatggaca aaggaaaaga tgccgatcca tgtgattgaa aagctgtttg
    48541 attaaaaaag gataatagat aaagatcttt tattctcgca taaacggtct caagaaaagt
    48601 aatcctttat gtatcgaagc atttatttcg ttatttttta ttaaattatc aaaaaatgat
    48661 aggcttttgt cgattggtta tcgtatcctt tatttatcag taagaaacga ggaggaacca
    48721 caatgagtcg gatggatcgc tataaggaca tccataagaa ggcgaaacct ctgaagcggg
    48781 atacggggag tatttttgct agaagagaac gaaaacgaac gcgtatggaa aatgaggaag
    48841 atgctacccg tatttatgaa agagagcagc attcatttga acatttgaac caatacgagc
    48901 aacagatata cagagatgca ccaagagcag aagaacaaca aaaaaataaa aaatttttca
    48961 atcggcaaaa gaagaacaaa ccgttaaaac ctcgaagaaa gcgatcgtgg aaaaaaatca
    49021 ttctatcgat tttgcttgtt ttgatccttt attcaggtat ctcatttttt attggtaaat
    49081 atgtagctga acatgacaca agcttagagg cagcgacttc agaaaccttt catgggcaaa
    49141 aagcgacgaa tggtgcgcat aatattttga tccttggaag tgatacacgc ggagaagatg
    49201 ccgggcgagc cgatacgatc atggtcttac agttggatgg tccttcccat aagccgaaac
    49261 tgatttcttt tatgcgtgac agcttcgtta cgatcccagg ggtaggacaa aataagatca
    49321 attcagctta tgcatacgga ggagccgatc tagtgcgtca aactttagta gaaaattttg
    49381 gaatcgattg tcaatactat gcgaaagtcg atttcaaatc gtttgaaaaa gtgatcgatg
    49441 ccttattcat gaacggagtg aagatcgatg cagaaaaaga tctgaatctg gatggtgtag
    49501 atattaaaaa agggggccag aagatggatg gacatgttct tttgcagtac gcacgtttcc
    49561 gaatggatga acaaggagac tttggccgtg tcagacgcca acagcaagta atgaacgcta
    49621 ttttcagcca attgaagaat ccattgaatc tgatccgtgc accttatgca gctggtaaag
    49681 ctttaggata tacatccaca gatgtcactg cttggttctt gacgaaaaac ctcttttcga
    49741 ttgcacgagg agctggtggc attgatcgaa taagtgtgcc agtagacggt tcgtggaatt
    49801 atgggcaaag tgattatgct ggcagtattc ttgaaattga taatcaaatg aatcaatctg
    49861 ctattacaga ttttttgaat aaatgatcat aaaaaagatc aagaagaagc tgcgcaaggc
    49921 ttttgtcatg aatcaatcaa acgattcact cgtaaaaaac gctttcttat ggtagattta
    49981 accaagagag gagctgttta cattggatat ctatctgaac ttcaaaaatg agtcgcgtga
    50041 agccatcgct ttttatgaaa aagtttttca gactgaagca tcaggtatca ccacatacgg
    50101 tgaaatgcct ggaatagaac aaatgaactt gccagaagaa gcaaaaaatc taatcacgaa
    50161 tgcacggctg aacatttatg atacattagt tatgtttgca gatacgccag aaggaattgg
    50221 tcctgaatta gttgtgggaa ataatgtttc tttagtcatc cagacaagaa atgaagaaga
    50281 aacgaagcgc ctatttgaaa gcttagctga gggtggtaag attgagatgc cactggatgc
    50341 tgttccgtgg gctcagttgt atgggatggt aactgatcga tttggcatca actggcagat
    50401 taaccaagag tgacagctaa agatgaatat tttctggaaa catcaagcag caaacggatc
    50461 atcgtttgtt tgcttgatgt ttttgtgtct atttcgacta tacggtatct tttttttatt
    50521 gccagatgcg aaaatatgtt ccataataga gaagtccgca aaaaattaag taacggaggt
    50581 cagaagatga agacggtgat actagctgaa aaaccttcac aagcaaaagc ctatgccgaa
    50641 accttccaac agcaaaagcg gcatgaaggg tattatgaaa tcagcgatcc attgtttgct
    50701 ggtgaagtaa ccatcactta tggttttgga catttggtag atatggttgc tccaggagct
    50761 tatgaagagc gttgggcaaa atggtcttta gaaaatttgc ctattttccc agaaaccttc
    50821 cgttacgaag ttccaatgga caaacgtgca caattttcga tcgtaaaacg agaattgcag
    50881 tcagccgata ccatcattat tgcaacagat ggtgatcgag aaggtgaagc catcgcgtgg
    50941 tcgattattc gacaagcaaa cgcattttct aaggaaaaga actatttacg tttatggatc
    51001 aattctcttg aaaaagaagc aatctatgaa ggttttaaga atctccaacc tggaaagaac
    51061 tattttccaa aatataaaga agcgcaagca aggcagaacg ctgattggct gattggaatg
    51121 aatggaagtc cattatatag cttgctactt caacgaaaag ggattgatgg cagtttttct
    51181 cttggtcgtg tacaaacacc gacactctat atgatttatc agctgcaaga agaaatcaaa
    51241 cacttcaaaa aagaaacgta ctttgaagga gaaggtttaa ttacgacaaa caaagggcag
    51301 ttttctggaa aaatcgtacc aaaaaaagca tttaaaaccc aagaagagtt aacgaaggaa
    51361 atagaaaaac ttggagcgca tttaggtaag caaaacggac ggattcttga agttacgaaa
    51421 aaggaaaaga ggttgcatag tccacgttta ttttcgctat ctagtttgca gacaaagatg
    51481 aatcagctaa tgaaagcaag tgccaaagct acacttgatg ctgctcaagg attgtacgaa
    51541 agcaagtttc taagctatcc aagaacggat tccttttaca ttacagaaaa tgagcatcag
    51601 tatctggtta aacatctgca aaattataaa aaatttttag gaatagaaga agtacagaca
    51661 actgagatca aagcaaagaa acgatatgtc gatgcgaaaa aagttcaaga acatcatgca
    51721 attattccga caaagacgat tccgactcct gcacgctttg cgaagctttc caaattacag
    51781 caagcaatct atttgcaagt tttgcgcact actgtggcta tgtttgctgc agattatatc
    51841 tacgaagaga cgaccgtaat gacgggcgta cagcaattaa agctaaaaag catcggaaag
    51901 atcccattac agcaaggttg gcaggaaatc ttacgcatca agtcaaaaaa gaaagaagct
    51961 cagactcttc ctcctatcgt agaaaatgaa gaagcagaag tagaactgaa aagtatggag
    52021 aaagaaacac aacctccaaa accctatacc gaaggaacgt tgatcacagc aatgaaaact
    52081 gcgggaaaaa cgctggataa tgatgaagcg caagaaattc tcaaagaagt agaaggaata
    52141 gggacagaag ctacacgagc caatatcatt gaaaatctta agcagaagca ttacatcgaa
    52201 gtcaataaaa acgaaataac tgttacgcca aaaggaatca ctctttgtaa ggcagtagcc
    52261 aatgaaccat tactgacgag cgcagaaatg actgcgcgct gggaagggta cttgagaaaa
    52321 attggtaaac aagaggggac accatctgtt tttttagaga atatcaaaaa attcatccta
    52381 catttattgg cagaagttcc taatcaaatc gacaaggtag acttttcaga tgagattagc
    52441 gggacacgtc agataaaagc gatggagaag aaaaacgatc aattaggaag atgtccaaaa
    52501 tgtaaaaagg gaatagtcat gctttatcct aaaatcgcta catgcttgaa tccggaatgc
    52561 gacttcaaat tgtggccaac agtagctaaa aagaagctga ctaaaacgaa tctcagagat
    52621 ttgctttcaa aaggaaagac aagtaaagtt gtcaaaggtt ttactggtaa aaaagggaaa
    52681 ttcgatgcag ttttagtatt aaaagaagat atgacaatcg gtttttcttt tccatggatc
    52741 gaaaacagtg gaacaaaagc agttgatgga aacaaagaaa catcaagaaa aaataatgaa
    52801 aacgaagtag agtaaatgaa aacaatgaaa tagcggaata tttattccga aaagtgagaa
    52861 tataaatacc aataaaacta aaaaatatca gtgaatcaag caaaaacgtt ggggtaggct
    52921 gattttatat agatagcaag gagttttttt atttgaaaaa agtgcattca tttttaaatt
    52981 atcttatggg agcaatcacg attgtggctg tgtaccaagc atttctaaag tatcctgatt
    53041 acgggacgaa gttgttgtca ttgacgcaca atcaaacagc atattcacca acgttgattt
    53101 tatcgacttt gactgcagga gctaaaagcg ctacttattt ttttcataaa acgacaagca
    53161 tgctaaagcg aaagaaacag cgaaaacaat tgttggtggt ggtagcggct atgatcatta
    53221 tttattttct accagttatt acacaagtat ttcatcaata ttttggttaa gaatacgaga
    53281 gtttgtgaca aaagtcatgt cacaaacttt tttctttgtc attttttc
//
LOCUS       DAHUVV010000008        52625 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.008, whole genome shotgun sequence.
ACCESSION   DAHUVV010000008 DAHUVV010000000
VERSION     DAHUVV010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.008"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            343..3150
                     /locus_tag="NK767_000470"
     CDS             343..3150
                     /locus_tag="NK767_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCH6693486.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            3342..3833
                     /locus_tag="NK767_000471"
     CDS             3342..3833
                     /locus_tag="NK767_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6693487.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3936..4925
                     /gene="manX"
                     /locus_tag="NK767_000472"
     CDS             3936..4925
                     /gene="manX"
                     /locus_tag="NK767_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCH6693488.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4958..5761
                     /locus_tag="NK767_000473"
     CDS             4958..5761
                     /locus_tag="NK767_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCH6693489.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5782..6696
                     /locus_tag="NK767_000474"
     CDS             5782..6696
                     /locus_tag="NK767_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCH6693490.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6779..7153
                     /locus_tag="NK767_000475"
     CDS             6779..7153
                     /locus_tag="NK767_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HCH6693491.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7292..7537
                     /locus_tag="NK767_000476"
     CDS             7292..7537
                     /locus_tag="NK767_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCH6693492.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7709..8773
                     /locus_tag="NK767_000477"
     CDS             7709..8773
                     /locus_tag="NK767_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCH6693493.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9019..9444
                     /locus_tag="NK767_000478"
     CDS             9019..9444
                     /locus_tag="NK767_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCH6693494.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9437..9886
                     /gene="nusB"
                     /locus_tag="NK767_000479"
     CDS             9437..9886
                     /gene="nusB"
                     /locus_tag="NK767_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCH6693495.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            9983..10837
                     /locus_tag="NK767_000480"
     CDS             9983..10837
                     /locus_tag="NK767_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HCH6693496.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10838..12175
                     /locus_tag="NK767_000481"
     CDS             10838..12175
                     /locus_tag="NK767_000481"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCH6693497.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12180..12404
                     /locus_tag="NK767_000482"
     CDS             12180..12404
                     /locus_tag="NK767_000482"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCH6693498.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12406..13296
                     /locus_tag="NK767_000483"
     CDS             12406..13296
                     /locus_tag="NK767_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCH6693499.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13305..14123
                     /locus_tag="NK767_000484"
     CDS             13305..14123
                     /locus_tag="NK767_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HCH6693500.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14230..14679
                     /locus_tag="NK767_000485"
     CDS             14230..14679
                     /locus_tag="NK767_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCH6693501.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14697..16379
                     /gene="recN"
                     /locus_tag="NK767_000486"
     CDS             14697..16379
                     /gene="recN"
                     /locus_tag="NK767_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCH6693502.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16434..16550)
                     /locus_tag="NK767_000487"
     CDS             complement(16434..16550)
                     /locus_tag="NK767_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HCH6693503.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16623..17579)
                     /locus_tag="NK767_000488"
     CDS             complement(16623..17579)
                     /locus_tag="NK767_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCH6693504.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17750..18118
                     /locus_tag="NK767_000489"
     CDS             17750..18118
                     /locus_tag="NK767_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HCH6693505.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18288..18719
                     /gene="mraZ"
                     /locus_tag="NK767_000490"
     CDS             18288..18719
                     /gene="mraZ"
                     /locus_tag="NK767_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCH6693506.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18740..19699
                     /gene="rsmH"
                     /locus_tag="NK767_000491"
     CDS             18740..19699
                     /gene="rsmH"
                     /locus_tag="NK767_000491"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCH6693507.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19704..20105
                     /gene="ftsL"
                     /locus_tag="NK767_000492"
     CDS             19704..20105
                     /gene="ftsL"
                     /locus_tag="NK767_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCH6693508.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20106..22292
                     /locus_tag="NK767_000493"
     CDS             20106..22292
                     /locus_tag="NK767_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCH6693509.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPSED
                     PDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYRNL
                     EADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVTGW
                     SKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22318..23280
                     /locus_tag="NK767_000494"
     CDS             22318..23280
                     /locus_tag="NK767_000494"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCH6693510.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23294..24655
                     /locus_tag="NK767_000495"
     CDS             23294..24655
                     /locus_tag="NK767_000495"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCH6693511.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVSECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24681..25769
                     /gene="murG"
                     /locus_tag="NK767_000496"
     CDS             24681..25769
                     /gene="murG"
                     /locus_tag="NK767_000496"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCH6693512.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25784..27004
                     /locus_tag="NK767_000497"
     CDS             25784..27004
                     /locus_tag="NK767_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCH6693513.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27160..28485
                     /gene="ftsA"
                     /locus_tag="NK767_000498"
     CDS             27160..28485
                     /gene="ftsA"
                     /locus_tag="NK767_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCH6693514.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28508..29749
                     /gene="ftsZ"
                     /locus_tag="NK767_000499"
     CDS             28508..29749
                     /gene="ftsZ"
                     /locus_tag="NK767_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCH6693515.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29766..30443
                     /locus_tag="NK767_000500"
     CDS             29766..30443
                     /locus_tag="NK767_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCH6693516.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30457..31056
                     /locus_tag="NK767_000501"
     CDS             30457..31056
                     /locus_tag="NK767_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCH6693517.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31071..31358
                     /locus_tag="NK767_000502"
     CDS             31071..31358
                     /locus_tag="NK767_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCH6693518.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31407..32189
                     /locus_tag="NK767_000503"
     CDS             31407..32189
                     /locus_tag="NK767_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCH6693519.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32210..32917
                     /locus_tag="NK767_000504"
     CDS             32210..32917
                     /locus_tag="NK767_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCH6693520.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33179..35977
                     /gene="ileS"
                     /locus_tag="NK767_000505"
     CDS             33179..35977
                     /gene="ileS"
                     /locus_tag="NK767_000505"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCH6693521.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36026..37552)
                     /locus_tag="NK767_000506"
     CDS             complement(36026..37552)
                     /locus_tag="NK767_000506"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCH6693522.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37567..38214)
                     /locus_tag="NK767_000507"
     CDS             complement(37567..38214)
                     /locus_tag="NK767_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCH6693523.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38398..38727)
                     /locus_tag="NK767_000508"
     CDS             complement(38398..38727)
                     /locus_tag="NK767_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HCH6693524.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38904..39632
                     /gene="sfsA"
                     /locus_tag="NK767_000509"
     CDS             38904..39632
                     /gene="sfsA"
                     /locus_tag="NK767_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCH6693525.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39648..40661
                     /locus_tag="NK767_000510"
     CDS             39648..40661
                     /locus_tag="NK767_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCH6693526.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40661..41938
                     /locus_tag="NK767_000511"
     CDS             40661..41938
                     /locus_tag="NK767_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCH6693527.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42001..44703
                     /locus_tag="NK767_000512"
     CDS             42001..44703
                     /locus_tag="NK767_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCH6693528.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44855..45172
                     /locus_tag="NK767_000513"
     CDS             44855..45172
                     /locus_tag="NK767_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6693529.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45202..45522
                     /locus_tag="NK767_000514"
     CDS             45202..45522
                     /locus_tag="NK767_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCH6693530.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45630..47090
                     /locus_tag="NK767_000515"
     CDS             45630..47090
                     /locus_tag="NK767_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6693531.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47158..47379
                     /locus_tag="NK767_000516"
     CDS             47158..47379
                     /locus_tag="NK767_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693532.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47410..47592
                     /locus_tag="NK767_000517"
     CDS             47410..47592
                     /locus_tag="NK767_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HCH6693533.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47592..48005
                     /locus_tag="NK767_000518"
     CDS             47592..48005
                     /locus_tag="NK767_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HCH6693534.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48128..49309
                     /locus_tag="NK767_000519"
     CDS             48128..49309
                     /locus_tag="NK767_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6693535.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(49380..49544)
                     /locus_tag="NK767_000520"
     CDS             complement(49380..49544)
                     /locus_tag="NK767_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693536.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            49698..49771
                     /locus_tag="NK767_000521"
     tRNA            49698..49771
                     /locus_tag="NK767_000521"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49732..49734,aa:Arg,seq:cct)
     gene            complement(49840..50979)
                     /locus_tag="NK767_000522"
     CDS             complement(49840..50979)
                     /locus_tag="NK767_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6693537.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(51093..51677)
                     /locus_tag="NK767_000523"
     CDS             complement(51093..51677)
                     /locus_tag="NK767_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCH6693538.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(51804..52235)
                     /locus_tag="NK767_000524"
     CDS             complement(51804..52235)
                     /locus_tag="NK767_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCH6693539.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(52253..52573)
                     /locus_tag="NK767_000525"
     CDS             complement(52253..52573)
                     /locus_tag="NK767_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6693540.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT        17385 a         8926 c        11166 g        15148 t
ORIGIN      
        1 ttactttgtc tttttacact taatattttt ttgccaattt ggaatcagga tgttttgata
       61 actatcgttt tcaattgatg gaagctatat tgtttgctat cagtgaaata attatatggt
      121 aaacagtgtt ctttttcttg tttccattat aaaaatcgta tcgggatttt tcgttctttt
      181 aaattgaaca tttctatcta tccgcgtgtt atcaacaaaa caaaatcagg taaatagttg
      241 acgaaacaga aataaataca tagtattatg ttttgaagcc taagtacaat agtctataga
      301 aaaggagcga taccattggc aaaacgcatt gaccgaattt atatgtatgt caaagaaaaa
      361 accgctcatt taacaaaggc agaatatgat caaggagtaa caacacagga aattgctgaa
      421 gtgttaggga ttcagcgaac aaatagcagt aaagatctga atcagctagt gcgcgaagga
      481 aaactgataa agacagacgg acgaccagta cgttacatat atcaaacact cgttacgcac
      541 gtaaagccag ttacaaaaca tgtagtcagt tacaaagaaa cgccggtctc tgaagaaaaa
      601 aatacactcc aaatagatac taaggatatc tttgcaaaaa tcattggagc taacggcagc
      661 atgaaaaatt cagtggaaca ggcaaaagct gcgattttgt atccgccaag aggattaaat
      721 tgtttgatta caggacctac cggatcagga aagacttatt ttgcacacgc gatgtatcat
      781 tttgcaaaag cgcatcatgt aatagatgag gaaacggaac tgattgtttt caattgtgct
      841 gattatgcca ataaccctga gcttttgatg agtcatttat ttgggtacgc aaaaggtgct
      901 tttacaggcg cagatgaaga aaaaatgggg attatcgatc aagcggatgg cggtatgctg
      961 tttttggatg agattcaccg gttgccgcct gaaggtcaag aaatgatctt ttactttatg
     1021 gatcatggaa cttacagtcg tcttggggaa acaacgaaat cccatgaagc aaatgtacgg
     1081 atcgtaggtg cgaccacaga agacccaggt tcttctctat tagaaacttt tgttcggcgt
     1141 atcccaatca atatcaaact tccggctttt gaaaaacgtc ctgcaaatga gaaagtcgat
     1201 ttagtaaaaa tcatgattgc ccacgaagca aaccgcacgc agcgaaagat ttctttgaca
     1261 gaagatgtag caaaagcatt gatcggcagt gtgacctatg gaaatatagg acaattgaag
     1321 tcaaatatcc agttagtatg tgcacgagga tttctaaatc atatgcaatc acccgaaatc
     1381 tcgatcacaa tcgatgattt gacagaaggg attcgtagcg gattgattca attagcaagc
     1441 aaccgcagtg caatggcaga gctttctaaa atattggagc caaagataac tgtagcacca
     1501 aatgatacga tcatgaaaat ccaatcggac tcctatgagt taccttataa tctatatgat
     1561 atcatcggag ataaagcagc tttactgaag tcggatggtt tggatcaaga agcaatcaat
     1621 catttcattt ctacagatat caacatccat ctgaagtctt tctataaaga ccatggtttt
     1681 tcttttaatg cagacagcaa attagcagaa tttgtagatc cgaaaatcat agaagtgacc
     1741 aatcagattt atgtaatggt caaaagatct ttaccatatg agtttcagca aaactttatt
     1801 tatgctatga gcttgcatat cagttctttt ttgaaaaaaa tcaacttagg agaagaacgg
     1861 catacgaatg ataacatccg tgagatggct tttgattttc cagatgaata tgctgttgcg
     1921 aaagaagtgc gtagttatat cgaaacgtat tttcaagtgg agattccaga aagtgaagac
     1981 tattacttga ccgttctact cgtctcgtta cgggcaaatc aagcaagcgg cagaatcggt
     2041 gtagtcgttg cagctcacgg aaacagcaca gcaagcagta tggtccaagt tgtccaacag
     2101 ttgttagatg cagagaacgt acgagcagta gacatgcctt tggatatgga tccgaaaaca
     2161 gcattggacc gaattgaacg caatgtacaa gaagtagatg aaggaagcgg ggtcatactc
     2221 ttagtagata tggggtccct tgcgtcgttt aattcgcaaa ttcaaagaga cactggaatt
     2281 ccagtacgta ctgttgatat ggtaacaact tctctagttt tggaaactgt acgaaaggtc
     2341 tcagtattag ggacagatct agatatgttg catgaatcat tgaaaaattt tcgaggttac
     2401 gcagaaatat cttctgtaga atctgttcag gaagcaaaac ctattcataa gaaaaaagct
     2461 gttttggcta tctgcgcatc tggcgaaggt acagcacaaa gaatcaaaga attgatcgaa
     2521 cgagcggtaa acaagcgtca agaaacagaa ttagaagttc tagctttgtc gattgtggaa
     2581 gtgaaagaag aactgccagt tatccaaaaa gaatatcaga tcatagctac tactgggatt
     2641 actgatttga atatccaagc cccatttatt cctttagaac gttttattga tcaaaatatc
     2701 gaagtcattt tggatcagtt gctgatggaa tcagaattgg aggaaactga attcatctcc
     2761 ctagatgaag agtcagctaa aaatacgtgt gttgagttta tctctgataa cttcatcttt
     2821 attaatggta gtaagttaat cgatccaatg tggcaatttt ctacacaaat ctctcagacg
     2881 acgggaatcg gcgatgaaga atatggattc aaaatcaacc tagtgatgca cacagcagga
     2941 atgatcgaac gtattattcg gaatgaacca ttgactgtgg aagaaaacga attgacaaat
     3001 acaacgaatg atcctcttta tagccaattg gcagcctcag ttgttctgtt ggaagatcag
     3061 ataaaagtaa aagtgccgat agaagaaatg tattatctgc tacgtctagt ccataatcag
     3121 ttagataaaa aagaatacac tgtgccatga atacacaaaa acgatacact tttatagtgt
     3181 atcgtttttg tgtattggtt aagaatcctt taaattcaat tagtgtatcg taaaaaaagt
     3241 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
     3301 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
     3361 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
     3421 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
     3481 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
     3541 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
     3601 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
     3661 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
     3721 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
     3781 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
     3841 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
     3901 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
     3961 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
     4021 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
     4081 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
     4141 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
     4201 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
     4261 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
     4321 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
     4381 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
     4441 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
     4501 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
     4561 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
     4621 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
     4681 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
     4741 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
     4801 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
     4861 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
     4921 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
     4981 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
     5041 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
     5101 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
     5161 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
     5221 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
     5281 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
     5341 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
     5401 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
     5461 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
     5521 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
     5581 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
     5641 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
     5701 aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga atgactatta
     5761 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
     5821 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
     5881 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
     5941 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
     6001 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
     6061 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
     6121 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
     6181 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
     6241 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
     6301 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
     6361 ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt atcaactgta
     6421 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
     6481 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
     6541 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
     6601 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
     6661 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
     6721 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
     6781 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
     6841 gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga
     6901 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
     6961 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
     7021 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
     7081 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
     7141 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
     7201 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
     7261 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
     7321 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
     7381 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
     7441 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
     7501 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
     7561 aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag
     7621 ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa
     7681 agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa
     7741 aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt
     7801 aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag cattttttat
     7861 tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa
     7921 aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc
     7981 attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat
     8041 cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga
     8101 agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat
     8161 cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta
     8221 tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg
     8281 ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac
     8341 attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat
     8401 tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc
     8461 agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga
     8521 ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg
     8581 tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg
     8641 aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga
     8701 ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt
     8761 gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt
     8821 tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag
     8881 ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt
     8941 tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc
     9001 aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat
     9061 ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct
     9121 aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta
     9181 ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta
     9241 gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag
     9301 gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc
     9361 catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa
     9421 gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt
     9481 gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa
     9541 cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt
     9601 ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat
     9661 tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat
     9721 cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt
     9781 aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa
     9841 cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt
     9901 ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca
     9961 aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa
    10021 aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg
    10081 attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga
    10141 acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc
    10201 tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat
    10261 attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg
    10321 ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa
    10381 accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat
    10441 cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat
    10501 ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa
    10561 tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt
    10621 tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga
    10681 tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg
    10741 gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac
    10801 cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt
    10861 cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt
    10921 atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag
    10981 cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt
    11041 acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga
    11101 agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt
    11161 atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc
    11221 taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc
    11281 ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt
    11341 gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag
    11401 agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga
    11461 ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc
    11521 ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt
    11581 tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt
    11641 gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg
    11701 caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt
    11761 gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca
    11821 gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt
    11881 ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt
    11941 agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca
    12001 atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt
    12061 aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca
    12121 ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata
    12181 tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag
    12241 aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa
    12301 gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag
    12361 aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac
    12421 ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat
    12481 acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga
    12541 atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc
    12601 gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat
    12661 ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc
    12721 ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc
    12781 agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt
    12841 gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag
    12901 ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat
    12961 gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa
    13021 cgattagccg aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt
    13081 ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact
    13141 tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc
    13201 gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg
    13261 cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg
    13321 tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag
    13381 tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa
    13441 ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg
    13501 gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt
    13561 tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa
    13621 tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta
    13681 gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag
    13741 gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc
    13801 cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg
    13861 aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa
    13921 aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa
    13981 gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag
    14041 tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac
    14101 acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat
    14161 tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc
    14221 gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa
    14281 gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact
    14341 caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat
    14401 ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa
    14461 aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa
    14521 acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa
    14581 ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag
    14641 gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc
    14701 tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg
    14761 aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt
    14821 taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat
    14881 gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg
    14941 aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg
    15001 gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg
    15061 gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat
    15121 cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt
    15181 atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa
    15241 atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg
    15301 ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca
    15361 attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac
    15421 aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag
    15481 atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg
    15541 caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag
    15601 aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg
    15661 agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag
    15721 aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac
    15781 aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc
    15841 tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag
    15901 aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca
    15961 atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga
    16021 tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg
    16081 atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc
    16141 aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg
    16201 ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt
    16261 cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga
    16321 ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag
    16381 ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg
    16441 acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg
    16501 acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac
    16561 ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt
    16621 ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt
    16681 aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt
    16741 ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt
    16801 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
    16861 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
    16921 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
    16981 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
    17041 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
    17101 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
    17161 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
    17221 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
    17281 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
    17341 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
    17401 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
    17461 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
    17521 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
    17581 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
    17641 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
    17701 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
    17761 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
    17821 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
    17881 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
    17941 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
    18001 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
    18061 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
    18121 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
    18181 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
    18241 gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg
    18301 aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag
    18361 aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga
    18421 tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg
    18481 cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag
    18541 gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag
    18601 tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg
    18661 cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag
    18721 aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga
    18781 aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg
    18841 tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt
    18901 tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca
    18961 aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca
    19021 tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga
    19081 agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc
    19141 agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat
    19201 tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt
    19261 acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat
    19321 cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg
    19381 gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt
    19441 attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt
    19501 caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat
    19561 acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga
    19621 ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat
    19681 aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg
    19741 atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag
    19801 aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa
    19861 ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc
    19921 gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata
    19981 aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga
    20041 ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga
    20101 agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga
    20161 tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt
    20221 tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag
    20281 ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga
    20341 tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga
    20401 tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg
    20461 ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata
    20521 ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa
    20581 atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg
    20641 gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt
    20701 ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg
    20761 agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata
    20821 attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag
    20881 atatctacac gactttagac agcagactac aaagttattt agaaacactg atggatcaag
    20941 taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag
    21001 aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg
    21061 gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca
    21121 tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct
    21181 tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa
    21241 aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac
    21301 tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa
    21361 gtactcattc cggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg
    21421 accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag
    21481 ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg
    21541 tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt
    21601 caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact
    21661 acggtagtgc ttatggtgtt gtacctggat acaatgtatc ggcaaaaacc ggaacagccc
    21721 aaatcgcttc tgatacaggg ggatatcaaa caggagatac agcctacctg tattcgattg
    21781 tggagatggt tccttctgaa gatccagact atgtcttgta tctaacaatg aaacacccta
    21841 agacatatga tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg
    21901 attttaaaga aactgaagaa gattcagata cagaaacaaa aacagaaaaa gtatccgtag
    21961 ccgattatcg taatcttgaa gcggacgttg cagcagctga tgctcaaaaa agtggactgc
    22021 aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc
    22081 agttgatctc tggagaaaaa ctgatcctat atactggtgg tgataagctg atgcctgacg
    22141 taacaggatg gtcaaaagca gatatcatga agctggggaa aatcctcggt attgaagtta
    22201 gttttgatgg agatggttat tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg
    22261 aagacaaact tagttttact ttagaagaat aagaagagaa ataggagaga aaagagcatg
    22321 gaatggacac aagcattgat tcccattgtc agcagttgtg caatgacaat tgccgcaatg
    22381 ccgctattta ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga
    22441 ccaaagtggc acaatagcaa agccggaact cctacaatgg gcggcttagt gtttctgatc
    22501 ggctctattt tgacaggaat ttgggttgga gcttggcaaa aacagctaac tcctactctt
    22561 ttcatcttgc tttttgtatt agcgttatac ggagtaatcg gctttcttga tgattttata
    22621 aaaatattca agaaaagaaa catgggactg aactcgaagc agaaattgat tggacagatt
    22681 ctagggggaa tcatctttta tcttgtttac cgttctgaag gttatccagg tgtattgaat
    22741 ttcttcggac tagatctacc tttaggaatc atttatggta ttttcgctct cttctggctt
    22801 gttggatttt caaatgcagt aaatctgaca gatggcatcg atggattagt tgctggctta
    22861 ggaagtatct cttttgcagc ttacggtcta attgcttggc atcagcatca gtatgatgta
    22921 ttaatcattt gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta caacagaaaa
    22981 cctgccaaga tttttatggg agatgtcggt tcattagcgt tgggcggact tttagcggca
    23041 atttctataa tgctaaacca agaatggaca ttgcttttga ttggtttgat ctatgttatg
    23101 gaaacagcaa gtgtgatgct tcaagttact tcctttaagc ttacaggaaa acgaattttt
    23161 aaaatgtctc caattcatca tcactttgaa atgtgcggat ggtcggagtg gaagattgat
    23221 acagtttttt ggttgatcag cattgtgact tcgttgatca cattatggat cgtttggtag
    23281 gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg
    23341 actcgcaaaa agcggagtaa gtgcggccaa attattacat gaattgggcg cacttgtcac
    23401 cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca caagagctgc tttctttagg
    23461 gataaaagta ataacaggta gtcatccaat cgaattgttg gatgaagaat tttcattgat
    23521 ggtcaaaaat ccagggattc cctacagtca tcctttagta gcaaaagccc aagaaatggg
    23581 tattccggta attacggaag ttgagctagc ttacgaagta tcggaatgcc caattattgg
    23641 gattacagga acgaacggaa aaacaacgac aacaacgatg acaggactac tactgaatgc
    23701 aggagcagat caaggaattg cacgtttagc gggtaatatt gggtatccag caagtggtgt
    23761 agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa ttgtctagct tccaactcat
    23821 gggaatcaca gatttccgcc ctcatatagc ggtgattacc aatatctatg aagcacatat
    23881 tgattatcat ggtacaagaa aagaatatgt caaagctaaa tggaaccttc aaaaaaacat
    23941 gacagaaaaa gattatctga tccttaactg gaatcagtca gaactacaag aattagcgca
    24001 aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag acggtgtgta
    24061 tgctgacgat tactcaatat actataaaaa agaaaaaata atggaaatat ctgaacttgg
    24121 cgtaccaggt aagcataatg tagaaaatgc actagctgct atttctgtag caaaattgta
    24181 tggtatatca aatgaagcta tacgagaaac gttgcatttt ttccatggcg taccacatcg
    24241 tacgcaatat gtaggagaaa tccaaggcag aaaattttat aatgattcaa aagcaaccaa
    24301 tattttagcg actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg tgttattggc
    24361 tggcggatta gacagaggaa atacatttga tgaattaatt ccttcattaa aaggaatcaa
    24421 agcaatggtc gtatttggcc aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg
    24481 catcgagaca atagtaacag ccgattctgt agaacaagca gttcctttag cattagaaaa
    24541 cagtacagat ggtgatgtag ttttattatc accagcgaat gccagctggg atcaataccc
    24601 aaattttgaa actagaggaa acagattcat ggaagctgtg aaccgattaa aatagaaaaa
    24661 gtagaagcga gcgataaaaa atgaaaattt tggttacagg cggaggaaca gggggacata
    24721 tttatccagc tcttgccttt gtcaattatg taaagactca agaaccgaat gctgaattca
    24781 tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt
    24841 tccatacctt ggagattcaa ggatttaaac gaaaaatttc catgcataac atcaagacgg
    24901 tccgattgtt tcttaagagt atccgtcaag caaaaaaaat cctcagtgat tttcaaccag
    24961 atatcgttat cggtactgga ggatatgttt ccggagcggt agtgtacgct gcagccaaaa
    25021 tgggtattcc aacagttatc catgaacaaa acagtgtacc aggtgttacc aataaatttt
    25081 taagccgata tgtggatcgg atcgctcttt catttaaaga tgcggcttcc ttttttcctg
    25141 aaaacaaagc agttcttgta ggtaatcctc gagcacaaga agtagcagat actaaaaaat
    25201 cagatatttt gaaaacattt gatttggatc cagaaaagaa aacagtcttg atttttggcg
    25261 gtagtcaagg agcacttaaa atcaatcaag cagtatcagt ttttttaagt gaatatattc
    25321 cttcttctta ccaagttttg tatgcttcag gagatagata ttatgaagag atcctaaata
    25381 aaataaatcc tgaaatacaa aatattagtg tccagcctta tatcaaaaat atggcagagg
    25441 tcatggcttc cagtgatctg ctggtggggc gtgctggtgc aacttcgatc gcagaattca
    25501 cagctctggg gttaccggca gtgttggttc ccagcccata tgttacgaat gatcatcaga
    25561 ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa aatgattgca gataatgaac
    25621 taacaggtga atcactaagt cagacagtca acgaaatcat gggtgatgaa gaattgcaaa
    25681 agcaaatgtg ccgagcttct aaagaacaag gaatccctga tgcttcaaaa cgtctttatg
    25741 acttagtaaa acaaataatc aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga
    25801 accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc
    25861 taaccgaaaa tatctggcag aacaagagat gaaaaaccaa gagccaaaaa aagaagacat
    25921 aacattagat acaccccttt cagattctga agaagaaaaa acggttcaag aggaacagca
    25981 agaagaaagc cagtccgaga aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa
    26041 cggtggacca tataacggtt cttttttgaa ccggctgccc aatttaaaaa gtcaacggca
    26101 caaagtacta tttcgtcggt tagctttgat catcactgta ttgggtatac cgctgatttt
    26161 tttgatctat tatgtttctc cttatagcaa attacaagca gttgcagtat caggcaataa
    26221 gacagtcaac agccaagaaa tcatctctga tacaaaattg tcattaggag aaaatgtttg
    26281 gggacagtat tttcatcgat ctacttatat cgatcgcctt aaaaaagcac agccaagaat
    26341 cgaaacagca aatatacatt tcaaagggat gaatgaattc gatttggatg taacagaata
    26401 taaagagatt gctttgatcg caaaaaacaa tcaatactac ccagtgattg aaaatggaac
    26461 agtattagat gaaaaagtag caaatcctac aaaaaatctc cctatcttgg aagagttcaa
    26521 agactctgca aaaatcaaag aattaacaaa gcaatacaat cagttatcat cagaattaca
    26581 aaaagcaatt tctgaaatca agtacacgcc aagagcaagc aataaaaatc tgatccaatt
    26641 gaatatgaat gacggcaatc aagttattgt aaatatcaat aatcttgcga accaaatgaa
    26701 atattattcg caagtagcca aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg
    26761 tatcttctcg catccttacg gtagcactca agaaacggaa gaatcgggcc aatcggaaga
    26821 aacaactgaa agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag aggcagaaac
    26881 gcaaaaagca caaacacctg gatcgtctag cacaaacgga tcatctgatt caaatgattc
    26941 aagtgaaaca agtcaaatag aagataatcc aagtacaact ccgtcaagtt taaatgaaaa
    27001 ttgataaaac aactttaaaa agtaagaatg tactttctaa aaaccagtaa tttatggtag
    27061 aattggaagc agtgaatata tctattaata agtatatttt aaaacggacg ctagttgttc
    27121 gcttgtatat ggaatagtaa gtaggaggga atcccattca tggcaaaaac aggaatgtat
    27181 gtaggccttg atattggaac gacatctgtt aaagttgtcg tggctgaata tatcgacagc
    27241 caaatgaata ttattggagt aggaaacgca aaatcagaag ggattaaccg aggcattatc
    27301 gttgatatcg ataaaacggt ccaagcgata caacgagcag tacgacaagc agaagaaaaa
    27361 gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa
    27421 aattgccagg gaatgattgc agttaacgga gattcaaagg aaatcaccga tgaagatgtc
    27481 agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca
    27541 atattgccac aagattttac tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg
    27601 atcggcgtaa gattagaaat gtacggtctg ttgtttacag gacctaaaac gatcatccat
    27661 aacatacgta aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt gattactcct
    27721 ttagcgctga ctgaatcaat tctttctgac ggagaaaaag attttggtac gatcgtcatt
    27781 gatatgggtg gcggccaaac aacaacggct gttatgcatg ataagcaatt gaagtttacc
    27841 aatcttgacc aagaaggtgg cgaatttgtc actaaagata tctctattgt attaaatacg
    27901 tcatttaaca atgcagaagc gttgaaaata aactacgggg atgcataccc agaacggaca
    27961 tcaccagatg aagaatttcc agtagatgtg attggtcagt cagaaccagt gaaagtcgat
    28021 gaacgctact tgtcagaaat tatttcagct cgaatggaac agattttcaa caaagcaaaa
    28081 gatgcattgg accaaatcga agcattagag ttgccaggtg gcgtcgtatt gacaggcgga
    28141 gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa
    28201 ttgtatgttc caaatcacat ggggctgaga aacccggtat ttacaaatgt aataagtatc
    28261 gtggattatt cagcaaactt gagtgaagtg tatcaattgg caaaaattgc tgttacgggt
    28321 gaaacctcag cagctcgtca aatggtagtt gaacaagaaa caacaaacac atatgagagc
    28381 tatgaagcac ctgaagaaac aatctacgat gagccggaac caaaagagtc cggagaaaat
    28441 gtaaaaaata aaatcaaggg tttctttaca aatatttttg attaattcat cctgggagga
    28501 ataagatatg gaattctcaa ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat
    28561 cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg
    28621 tgttgaattc attacagcca acacagacgt gcaagcatta aaaaattcaa aagctgaaac
    28681 agttatccaa ttaggcccca aatatactcg tggtttaggt gctggttctc agccagaagt
    28741 tggacaaaaa gcggcagaag aaagtgaaca atccttacgc gaagctttag acggtgcaga
    28801 tatgattttc atcactgcag gaatgggtgg aggaaccggt acaggtgctg caccaatcgt
    28861 tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac gtccattcac
    28921 ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa
    28981 tgtggataca ttattaatta tctcaaataa ccgtttatta gaagttgtag ataaaaagac
    29041 accgatgctt gaagctttcc gtgaagcaga taatgtttta cgacaaggtg tacaaggtat
    29101 ctctgatttg atcacagcgc caggttacgt taaccttgac tttgctgacg tgaaaacagt
    29161 gatggaaaac caaggtacag cattgatggg gatcggtgtg gctagtggag aagatcgcgt
    29221 agtagaagca acgaaaaaag caatctcctc tccattgctt gaaacatcta ttgatggagc
    29281 tgaacaagta cttcttaaca tcacaggcgg tttggatatg acattgttcg aagcacaaga
    29341 tgcttcagat atcgtagcaa atgcagcaac tggcgatgta aatatcattc taggtacatc
    29401 tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag gtatcgatga
    29461 atcaaaaaag gaacgcaagt ctagccgacc agctagacaa gcacaaatgc aatcacctgc
    29521 tcaaaagact gtattagata tggatcaagc taaaccaact tcttcagaag aagaaaacag
    29581 ttttggcgat tgggatatca gacgtgaaca aaatgttcgt cctagagttg atgattccaa
    29641 ttttgacaat atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa caaaatctca
    29701 tggcgatgat gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag
    29761 agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc
    29821 ttgttcatcg tgatccgaaa gaagttacat tagtagctgt aacaaaatct gttgaatccg
    29881 ctcaagctga agaattggtt agagctggcg ttatgaattg tgcagaaaac cgagtagata
    29941 aattattaga aaagaaacaa gatttatttg attttcctga aataaagtgg catttaattg
    30001 gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga aattgattac tttcatgcgt
    30061 tggacagttt aagattagca gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt
    30121 ttattgaagt aaatgtttct ggtgaggaat caaaacatgg aatttcacca gatgagcttt
    30181 taccgtttgt ggaatcttta gctgcctgca caaaaatcaa ggttgttggg ttgatgacta
    30241 tggcgcctaa agaagcttct gatgagcaaa tccataaagt gtttggtacg ctgaaagaat
    30301 tgcgtgatct tgttcaagca aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg
    30361 ggatgagtca agactttcaa attgcgattg aagaaggtgc aacatttatc cgtgtaggaa
    30421 cagcgctatt caaagacgaa taaaggagga acaaaaatgc cgcttttaaa cagaggagca
    30481 attgctaatt ttttcggatt agctgatgag gaagagtacg aataccaaga ttatcctgaa
    30541 acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa cacagaatca atcaactcat
    30601 tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg
    30661 catgaaagta ttcagccggc tgaaaagaaa aaagtaattg aactacatca atcacctaca
    30721 aaaactgtag cgaaagaaag aacagcttca agaacctcta cacaacctaa agcaaatctt
    30781 gctggaaaaa tcactgtaat ggaaccaaga gcttattctg aagctatggc gattgctaaa
    30841 cgaatcattg ctggagaagc aaccttagtg aattttcatt tagtagaaga aaaacaagcg
    30901 agaagaatcg ttgattttct aacaggaaca gtttatgcac tagatggaga tatcaaacgt
    30961 gttggagatg agatttttct ttgtacaccg actgggatgg agattgacag ttcaaccgct
    31021 caatcttttg cagatgcgaa tttgtttgat ctgtaagagg aggaactacg atttatacta
    31081 ttgcactatt acttcattta cttcaacggg cagcctattt ctatagcatt atcttgatgg
    31141 tatatgcatt gctctcttgg tttccaggcg ggtatcaatc agggctagga cgattattag
    31201 ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg attggaccct
    31261 tggatttcac aattttaggg gcaatcctga tcttgaactt agccgtacaa ggtttagcac
    31321 agatcgttat atggataact gtccatttag tttattgatt gaagcaacaa aatctttatt
    31381 ctcagaatag ataggagggg aaaattttgg atgcaaatgt ataccagcac tttcgggcag
    31441 atgaacgtac atttatcgat tcggtaggcg attggattga acaggttcaa ggacaatatg
    31501 caccctatct gaccgaattt cttgacccaa gacaatccta tattttagaa acactgattc
    31561 gacaaagcag tgatttgcgt ttcatgtttt atggcggata tgaacaggca gaaagaaaaa
    31621 gatgtttgat tttccccgat tattacgaac ctgtagagga agattttgaa acagcattgt
    31681 atgaaatcca ttatcctagc aaatttgcca gcctaagtca tgggaaaatc cttggaactt
    31741 tagtgggaac ggggatcaaa cgctcttact ttggcgatat catctcggac ggtgatcgct
    31801 ggcaagtatt tattgcaaat gaggtggatc attttgttgc tgcacaagta acaaagattg
    31861 gaaaggttgc tgtacgcttg gaaccaatca aatacacaga aatgattatg ccgaaagata
    31921 gctggacaca agaaagagga acagttagtt ccatgcgact agacagtgtg atctctgaga
    31981 tttataatat ttccagacag cggtccaagc agctgatcga agctggtaaa gtaaaagtga
    32041 actggacaga aaatacaaga cctgattttg tacttgaact attagatatt atttcagtca
    32101 gaggttatgg cagagtccag atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca
    32161 gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt ggtttactca tggctttaac
    32221 tccattagat atccaaaata agacattccc gacaaaaatg cggggatata atcaagacga
    32281 agtagatgat ttcttagatt tagttgttcg tgactatgag gaattgactc aaagaaatcg
    32341 agaattagaa aaagcagtca aacattctga agaaaaactt gagtacttca atgaattaaa
    32401 agatgctttg aaccaatcaa tcatcgtagc tcaagatacg gctgataaag taaaaacaag
    32461 tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa aacaaagcag atgagttagt
    32521 tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat gcagaagaaa aagcaagaaa
    32581 aatcttgaca gatgcaacag aaaaagcaag acagcttgcg acagaaactg aagatcttaa
    32641 gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg cttgagtctc aattagaaca
    32701 agtgaaaagt ccggaatggg atgaaatttt acaaccattc tccagctacg tgactgacag
    32761 ccatgaagtg atcaaagaag ttttagcaaa acagcttgac aatgaaaatg atactgatgt
    32821 aaactcagac acaggagtca tagaagcacc tatgacggca tttgatacac aggctattga
    32881 tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac agaaaagcac tttgttattc
    32941 ttaaagcgag ccgatgagag tggaagttcg gtaagacact gcatggtgaa agatcctcta
    33001 cttgagacct gcttttccga aattagagta agaagcagcg gctgacctcg ttattggtca
    33061 cagagagttt gcactagata gatgcaacta aatttgggtg gtaccacggc atgttcgtcc
    33121 cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat
    33181 gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc
    33241 aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca
    33301 agagttgaac gaaggaaaac ctagctttgt tcttcatgat ggacctccat atgccaatgg
    33361 taatatccat ttaggacatt cattgaataa aatcagtaaa gacattatca ttcgttcaaa
    33421 atcaatgtct ggctatcgtg caccatatgt tccaggatgg gatacacatg gattgccgat
    33481 tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt
    33541 ggaaaaatgt cgtgaatatg cactttccca agtagataag caacgagcag atttcaaacg
    33601 cctaggagtt gcaggagatt gggaacatcc ttatgttaca ttagatcctt cttatgaagc
    33661 tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaagc tatatttaca aaggattgaa
    33721 accaatttac tggtctccgt caagtgagtc ttctttagcg gaagctgaaa ttgaatataa
    33781 agatgtaaaa tctccatcta tttttgtagc tttcaatgtc aaagacggca aagatattct
    33841 tgatgaagat acagcttttg tcatttggac gacaacacca tggacattgc cagcaaacca
    33901 aggtattgct gtaaatccaa aatttacgta tgtattggtg gaagccgatg gccgtaagtt
    33961 tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa atcggttggg aaaatgtaac
    34021 agttctaaaa gaatttgctg gacaagaaat ggaatatatg actgcaagtc atccattcta
    34081 tgaacgtgaa tcacttgtga ttttaggcga tcatgttact ttagacgctg gtactggatt
    34141 agtccatact gctccaggac atggggaaga tgactatatc gctggtagca aatataaact
    34201 atctgtagtg tcacctgtag actcaaaagg attgtttaca gaagaagcac ctggatttga
    34261 aggtatcttt tatgacaaag caaatcctat gatcactgat ctattaaaag aaaaaggagc
    34321 tttgctgaag ctagatttct ttactcatag ttatcctcac gattggcgga cgaaaaagcc
    34381 tgtgatctat cgggcaactc cacaatggtt tgcatctatc gataaattcc gtcagaatat
    34441 tttggatgaa gtagaaaaag tagagtgggt tatcccatgg ggtaaaacac gtttgtataa
    34501 tatgatccgt gaccgcggcg attgggtaat ttcccgtcag cgtgcatggg gagtaccact
    34561 tccgattttc tatgctgaaa atggagaagc aatcatcaca ccagaaacaa tcgaacatgt
    34621 ggcaaaatta tttgctgaac atggatcaat tatctggttt gaaaaagaag cgaaagaact
    34681 tcttcctgaa ggatttaccc acccaggttc tcctaatggc gaatttacaa aagaaaaaga
    34741 tatcatggat gtctggtttg attctggttc ttctcatgaa gctgttttac gtcagcgacc
    34801 agaattatca ttcccagcag atatgtattt agaaggatca gaccaatatc gtggctggtt
    34861 caactcaagt atcacgacta gtgtggctat caatggtgta gcaccataca aatcagttat
    34921 ttctcaagga tttgctttag atggtgaagg ccgtaagatg agtaaatctt taggaaacat
    34981 cattgcacca gaaaaagtga tcaaacaatt tggtgcagat atccttcgct tatgggtatc
    35041 tagtgtagat tatgaagcag atgttcgtgt ttctatggat atcttaagtc aagtggcaga
    35101 agtatatcgt aaaatcagaa atacgatgcg tttcttatta gcgaatacag aagatttcga
    35161 tcctaaaaaa gatactgttt cgtacaatga tttacgttct gtagacaaat atatgaccgt
    35221 tcgattgaac caagtaatca aagaaatccg agaagaaggg tacgacaaat ataacttcat
    35281 gcatatttac cgtacagtta tgaacttctt gacggttgat ctatcgtcat tctacctgga
    35341 ttttgctaaa gatgtagttt atattgaagc agaagatgat tatcaacgtc gttgcatgca
    35401 aactgttttc tatcaaacag ctgtagcttt gacaaaatta ttgactccta tcattccgca
    35461 tacagcagaa gaaatctggt cattcctgaa agaagaagag gagtacgttc agttaagcga
    35521 gtttcctggt tatgaagagt ttgcaaacca agaagaattg atggatacct ggactgcttt
    35581 catggacttc cgtgataatg tcttaaaagc attggaagaa gcaagaaaca gcaaattgat
    35641 tggtaaatct ttggaagcaa aactaactgt ttatccaaaa caacaagttc gtgaaatgtt
    35701 aaaagctttg gatgcagata ttgcacaatt attgatcgtt tcaccagact actttgaagt
    35761 tatggcagca gatgaacaag tgccagataa cgctatggta tttgatgatg ttgctattac
    35821 agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc caagttcgta aagatgtagg
    35881 agtagatgaa aaacttcctc atctatgcgg tcgttgtgca aaaatcgttg aagacttcta
    35941 tcctgaagct gtagctgaag gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg
    36001 ttctttgtca cacccccttt tgttatcaat cgtttaattt tccacgttca cgataccagt
    36061 tatctggttg ccagatccat tcgaatccgt ctttttcaag tagttcgaaa gctgctttag
    36121 gtcccattga tccagctttg tagttgggaa aatccggttg tgtttggtcc caagcttttc
    36181 gaatgacatc cacgatatgc caagattgag cgacttcttc ccaatgagag aagttcgttc
    36241 catcaccatt caacgcatct aatagtaatt tttcgtaagc ttctgggcta ttttcaacca
    36301 tttctgcact atttctaaat tccaatttga ttggttccgt ttcaaatcct tgccctactt
    36361 ctttcccatt caaggataaa gaaaatcctt cagttggctg aatatagatc gttagaacat
    36421 taggtggaag tgtcgtatcg tcacatggtt catcgacaga tgttttgaat acattaacag
    36481 gaacttgttt gaatacgatg ttgatccgag tccctttttc agtcaaacgt ttgcctgtac
    36541 gtacatagaa aggaacacct gaccaacgga agttatcgat agtgaatttg cctgcgacga
    36601 atgtttcagt ctctgattga gcatccactt tgtcttcttc gcgatagccc ttgaattgta
    36661 caccatccaa ttcaccttga gcatattgcc cgcgaacgaa gttttctaac acttcttctt
    36721 cgttataaat gcggatagct ttcaatgctt tgatcttttc tgtacggatt tctttttcgg
    36781 aaaatgctac tggcggttcc attgcaagta atgctacaac ctgtaaaata tgattttgca
    36841 ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc gcgatcttct acaccgaggt
    36901 tttcgccaaa agtaatttgg atattgtcga tatagcgatt gttccattga gcctcaaaaa
    36961 tattattggc aaaacggata gctgagatgt tttggatcat ttcttttcct aaataatgat
    37021 caatcctgaa aatatcttgt tctgggaata cttctctgat ttgctggttc aattcaaaag
    37081 cagactggta atcagaaccg aacggttttt ctatgatcaa acgattgaag ccattttttg
    37141 tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag
    37201 ccaaataata gacacggtta ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt
    37261 cttttaatgt ctgataatgc gttgaatcat ttacatcatg ggattgatag taaaaatggc
    37321 ttgcaaaggt agttgcttct tcatctgaag gattcaatgc acggattgtt tctttgacaa
    37381 cttcacggta gtgctcatcg ctccagggac ggcgagcggt tccgattaca gcaaaatgct
    37441 cgcctaaatt tccttttctg taaaggcgga acaaagacgg gtacaattta cgctgggcta
    37501 ggtcacctgt accgccgaat atcgtaaaca atacattttt ttcattagac atgtggatac
    37561 actcctttat attttatcga caaaaatcgt gctggccgcc ttaaagctga cggggatttt
    37621 ctttctatca ttttggatag tgatcggtcc ttcataagct gcgatgtcaa cgatctcata
    37681 ttcttcgttg atattcaggt caatagtgac taagtaatcc aacaattctt tttcatcaag
    37741 cactcgtgcg atacgcactt ttgtaccgac aggataggac tccaatgttt gtcggcgttc
    37801 ttcatggaca acttcctctt ttttaggaat cgatcctcca tgaggacaat gttcaggatg
    37861 attcaaatag ttttctaaat gatctgcaag atgttccgaa gtcacatgtt ccaatacttc
    37921 agcatcatca tggacttcat tccagctata gtctaaatgt tcaactaaaa aaacttccca
    37981 taagcgatgt ttgcgaatca gactgctagc tttttgcagt ccgcttgcag tcaattgtac
    38041 accttgataa ggggaatgtt ctacaagctc ttctttcaca agtttagaaa tcatttcact
    38101 gacagatgca gctgagacat ctagactaga tacgatttgt ttattgttga ctttcgttgt
    38161 atctccacct agttctaaaa tgattttcaa ataatcttct cgatttggtg tcatattcga
    38221 tcatcctttc atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac gttaattgac
    38281 tatttttcta aataaaaaac aaaggatttt tagacgtttt tacaacaaaa aacgaaagat
    38341 gatagagaag actcattctt tcgtttataa aggatatata tatacattgg gagggatcta
    38401 tttttcagat aacaacttat atgaagtgtc tgcagattcg tcataaagag tttgttcggg
    38461 ataagaaaag ataagataaa aaccatactg atcacgtacg actaaacgat attcatctat
    38521 ttctaccaca gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat cagatgtaat
    38581 ctgtaattgt tgtttttgct ggctatcact aaagaaccat gtaccttgat actttgagag
    38641 tccttctgac ttttccgctt gttttttctt tttcaagtaa ataaaacttg agagaacggt
    38701 aatgagtgaa ccaagaaaat agatcatttt ttttatcacc aaatcactcc tttatgagaa
    38761 aataatcgca tagacatatt ctttcttctt tatttcattg taccgaataa aagctaaaat
    38821 ttctctaaga ctttcgactg gtttttttac tgtttttcta caaattatga taggatacat
    38881 agtaggaaga aaggtgacat aatttgaagt atcagcaagt ggaaatagct ttttttattg
    38941 accgcccgaa tcggtttata gcacattgta tgaaaaaaga cggagaaata attattgcgc
    39001 atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa gtggctattg
    39061 tatttgctcc tggacctaaa cggaaaaccg cctatgatct gattgctgta aaaaagaagg
    39121 gtgactggtt taatatcgat agtcaattgc ctaatcggct ggcaattgat ggtattttag
    39181 acggcacgat ccttttacca ggattaactg gtccatttac ggtatacaag cgtgaagtaa
    39241 cttatcttca ctccaaattt gatatttatt tagagacggt ggtcggacaa aaagcatttg
    39301 ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg tgcttttcct gatgcgccca
    39361 cactcagagg attgaaacat ataggagaat taacgtatgc cgcacaagat ggttatgccg
    39421 cttatgtctt gtttattgct cagttcgaac atcttcatct ggcaacgatc catgaagaaa
    39481 tgcaaccagc actagctgat atggttcgac atgcgcagca aagcggagtt caaatcctcg
    39541 cctataattg tcaagtatcg gcagatgagg taactgtcaa gcaggccatt ccatttgatg
    39601 tagatatccc ttttgaagat cctatacaat agaaagaagg taaaataatg atcaacttag
    39661 gtattatcgg aacaaattgg attactcatc aatttgtcca agcagcttta agcacaaaaa
    39721 aatatgaatt gacagcggta tattctagac atttggaaaa agcacaggaa tttggttctc
    39781 attatgaagg ggatatcgaa ttcgcaacag atttgaagac ttttttcaat attactcata
    39841 tggacaccat ttatattgct tctccgaata gtcttcactt tagtcaagct aagcaagcaa
    39901 ttttggcagg aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca aaagaaatgg
    39961 atgaaatcat ccatttggca aatgaacaca atgtgtattt ttttgaagca gcaagaaata
    40021 tccatgaatc aggttttaaa aagattgccg attacttacc attaaaagac gagataatcg
    40081 gtgcaaattt cagctatatg aaatattctt cgcgctatga ccaagtatta gatggagaag
    40141 aaccgaatat cttctcgact catttctcag gtggtgcatt gatggattta ggtgtttacc
    40201 ttgtatatgc agctgttgct tggtttggaa tgccaaaaga agtccattat ttcccaagaa
    40261 agatttctac tggtgtagat ggtcttggat gggggatctt acgttatgag aattttgatg
    40321 tatcgatcca gccaggtaaa atcggtgatt cttatttacc gtcagagatt tattttgact
    40381 caggaacgct agtgatgaat ggagtcaatg caattgaaca agcacaattt catgatcgcg
    40441 cgcataaaga aatcattgat ttggcggtaa cagctgcaga aaatccaatg gcagatgaag
    40501 cagaggattt tgccaatgtc atggctcatc cgaaagatcc agcttgggga ttgttgtatg
    40561 aagagtggat cgagctagct agaaatgtga accaagtgat ttatgattta agaaaaaatg
    40621 gcggcatcaa attcgatgca gataatgagg aagattttta atgaacgaat ttgtacaagt
    40681 atttcccgaa aattggcaga aaaaatatga ggataaaggg tttatctcac cttctcttat
    40741 tcaacgagca gtttttcagc cactaagtaa taaacaaagt atagtagcgg tttctcctac
    40801 aggttcaggt aaaacactgg cttatctttg gcctctacta ctaaacgtgg aaccaggtga
    40861 agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta gctattcaag tagcagatgt
    40921 tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa tctttggtgg gcggagccaa
    40981 cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa atcttgattg gtacaccagg
    41041 aagaatattg gaattgatga aagcaaaaaa aataaaagca catcaagtga aaacaatggt
    41101 atttgatgaa gcagaccagc tatttgacgc tgggaacagc caaatcattg atcaaattct
    41161 tcatcaagct ccaacagagt atcagctagc ttttttttca gcgacagcag atcgttccct
    41221 tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca attgacgtga cagcagagga
    41281 cgattctcga aaaggactga gacattactt tttacgtgta ccgatacgaa agaaagatga
    41341 ctacttgaga cgtctcacgc atatagaaga ttttcatggt ctgattttct ttaatcaact
    41401 gactgaatta ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg taggaagtct
    41461 ggcctctgat cagaacaagc tactccgaaa agcagcgcta gaacaattca aaaagaaaat
    41521 ggtcagagca cttttgacca cagatgtggc ggctagaggt ttagatatta caggtcttcc
    41581 ttatgtagtg aatgcagaag ttccgttaag tgaagaagct tatttgcatc gttcgggaag
    41641 aactggtcga atgggtaatg aaggaatagt aattacactc gtacaagata ataatctaag
    41701 agatttaaaa cggcttttac gtccatcaaa tataacactt gaagagattt ttttatacgg
    41761 aggtaagctg caaactgagc agccaaccaa agaagatgca gctcctgcag taaataaaaa
    41821 gtatgcgaaa actttttctg gaaaggagca gaacccagaa tataacaaag aaaaaataag
    41881 aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc
    41941 gcctttttat gctatgatta tgaaaacggg aacaaaattg gttgccagag gaggggttat
    42001 gtgaaagaac gtcaagaaga gatcttgcag ctattgacag aaaaccaaca aggattgaca
    42061 gcaagtgatg tagctgagcg cttaactatc gatcgaagca atgcaagccg atatttaagt
    42121 gaactataca aggcccatca tattgtaaaa accgctggcc gaccagtcgt ttactctttg
    42181 ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact cttctacgca agtgactttt
    42241 gaaacactcg ttggagaaaa tgattcttta aaagtcagca tacagcaggc aaaagctgcg
    42301 attctttatc caccgcgggg gttgcatacg attatttttg gagaaactgg tacagggaag
    42361 tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt cagagatgct ttctgcagat
    42421 gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa atccgcaact gctctttgga
    42481 cacatctttg ggatcaaaaa aggggcttac acaggagcag ctcaagatag tcccggtcta
    42541 atagccaaag cagacggcgg tatattgttt ttagatgaga tccaccgttt accgccggaa
    42601 gggcaggaaa tgctgtttac tttcattgat aaaggtattt accgtccgct tggagaaagt
    42661 gcacaagtac atgaagcatc tgttcagatc attggggcga cgactgaatc ttcagaaagt
    42721 ttcttatcta cgttcaatcg acggattcct atggcaatca ccttgccgaa tcttgcttca
    42781 cgttcactgg atgagcgtta tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt
    42841 ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg
    42901 gaaggaaata tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa atctttttta
    42961 cattaccgta ctcatcatga gaaaaagcta atcattcgaa aagaagaact ttcccttcaa
    43021 gtacaaaaag gattactgaa aatcaaggaa ataccggaac gtctcgaccg attcatggac
    43081 agcaaaagtc agtatttgac atttgaacca ggatttgctg atgttgtctg gtcacaagat
    43141 ccagaacgaa acatgcaggt ctataacgat attgaggaaa aagtcacttc tctttccgaa
    43201 accggtgtgg agaacattga tctagagacc ctcatttcaa aagatgtgga tgcttatttc
    43261 cagacatatg tcaaagaatt gactaaaagt tcgattccca aagatctttt gccagatgaa
    43321 atctggcggt taaccaatca attgtatgat gtggctgaaa aacggttaga ccgtatttat
    43381 aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa gtacattgga tcgtgtgaga
    43441 gaaggtcata tgatcgtcca cccagatttg aataacgtac ggaaaaattt taagagggaa
    43501 ttccaggtag caatcgatct ttcaacgatc attgaagaag aagaacaagt tgagattcct
    43561 tttgatgaaa tcggctttat tagtatgttt ctttctatca atctaggaga aagtgagcct
    43621 gttaaagaaa acaaagtaga tgttctcata ttgatgcacg gcagacagac tgcttcaagc
    43681 atgttggaga tggcacaaga actattagga acaaaagtag ggaaagcttt taatatgcca
    43741 ctggttatgg aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa
    43801 gagcatttga cgaatggcct actcttgtta acagatatgg gatcattaaa caccttcagt
    43861 gatttgattt atgaagagac tggcattcgg acgaaagcta tcacgatgac aagtaccatg
    43921 attgtgatag aagcgatacg gatggcaagt gttggaagaa gcttagaaga tatttatcaa
    43981 aatatccagc tttcctttga atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa
    44041 actttacagc gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc
    44101 aaattatatc agcgtattat gccagtgatt aatcaaaata aggtagaact gatccagatg
    44161 cagtttttag aacgtgaatc attcaaaaaa cacatcgatg ccttattgga agaatatgag
    44221 atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa atatcccatt tttttcagcc
    44281 tatgacgttt ttgatgatga aaaactaaat attttaaagc gtatagctga tgatgagatc
    44341 ccgacagcta aaattgtcga ttcacttgct ggatcattaa gcgatgtctt ttctattgat
    44401 gaactgatcc gttcattgca aaagacagtt cagcagatcc agaccgattt gtcgattatc
    44461 gtggaacctg gagtagatac tggaatcgtt atccatttag ccttcatgtt agacggattg
    44521 atcaaacaag aaagccatcg gcaatttgag aaactgtcta ttttctcaaa aatttatcgt
    44581 ttggaaatgg atatcgttcg ggcaaacttg atggcactag aacgaaaata tcaggtaaaa
    44641 attccagaag atgatatcgc atttcttaca caaatgtttc ttgaaaataa aataaaaaaa
    44701 tgaacacact aatcatactg tttacacact aaacatctgt gtgcgcacta taaaaaagag
    44761 ctaaaaagca tgaaaacgct atttcagctc ttttttttat tggcatgctt cttgcgttat
    44821 ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt
    44881 ttgttcagca gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga
    44941 aaaagggatg gaagccgata tttttgcggt ctctgcctca gatgcagata ataatttaga
    45001 atctaagaat gtcgatgtat tattattagg tcctcaagta cgtttcatga aagctcaatt
    45061 tgaacaaaaa cttgcgccta aaggtattcc attagatgtc atcaatatgc aagactatgg
    45121 catgatgaac ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta
    45181 tgcagttggg ggtataacaa cgtggaagat caacaaaatc tagaagccat catggggtta
    45241 atcatgtttg gcggtaatgc aaagagcgat gcaatggaag cgattgcggc tgctaaaatc
    45301 ggtgattttg agttagcgga taggaaaatc gctgatgcag aagaatcact tatacaagcg
    45361 catcatgcac aaacagacat gttgacacaa gaagcacaag gaaatcatat gcaagtgaca
    45421 ttattgactg ttcacagtca agatcactta atgacatcta ttgcgtttac tgatttagca
    45481 aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat aaatacaact tcttttttga
    45541 acggaagaag aaaaattttt gaacggattt tttattttgt tataaggaga actctcttta
    45601 tataaaaaac ttataggagg aagtaaaaaa tgaacggact atcaagttgg atggagaaat
    45661 acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca tttagttgct ttgcgtgatg
    45721 cttttatcgg cacactgccc gcaacaatgg cgggatcagt cgctgtaatg atcaatgcaa
    45781 ttattcgtga tttaccgcaa cagtttaaca gtacctatgc tgacagttta gcagcagaca
    45841 aaggaatctt tgccgtattg aatgtcatca tcggaatcaa tggttttgtc tggaacggta
    45901 cactagcaat cgccggattg atctttgcct tttcttgggg gtataatctt gcccgagctt
    45961 atggagtaaa tgatctagct ggggggatcg ttggtttagc taccttgatt caaggtattt
    46021 cctttggtcc agcattatca gcagcattag atgtgaatgt tccttcaaat gtcgcagatg
    46081 caatcaatag ttctgagatt ggtgcaacga ttgcagatgg cacactgtca gttggattat
    46141 ggggttggct aaaactagat catttgaatg gaaacgctta ttttacagtt atgatcatgg
    46201 gagctatttc agtcattatt ttttgtaaat tgatgcttgc caacattacg atcaaaatgc
    46261 ctgattcagt accaccagca gtatctaaag catttgcagc cattttacca gcgacgatcg
    46321 ctttatacgt cattgctatt atcaattata tcgtaggtcg tgtagctgat gggcaactga
    46381 tcattgattt ggtgcaaaaa tatattgcag aaccattcct aggattatct caagggttgg
    46441 gagcagtatt gatcgtcaca gtatttgttc aagtattctg gttctttggt attcatggac
    46501 caaatgtatt ggcaccagtg ctagagggaa tctggggaca agcacaacta atcaatattg
    46561 atattttcca aaaaggttat aacggaaaaa ctggtacacc agccgtgtta gatgcaatcg
    46621 aagatggaaa agcttatatg tgggtccgcg gatcgttcga tgcttttgca tggtttggcg
    46681 gatcaggcgg aacgatcgtc ttactgatag ctatcttgat tttctctaaa cgtgcagatt
    46741 atctaacagt agctaaatta tccattgggc ctggtatctt caatatcaac gaaccagtga
    46801 tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat cccattcttg attgctccag
    46861 tagtagcaac agcaattgga tggacagcta cttatcttgg cctagtcgct ccggtatcgc
    46921 agcaagtcac atgggtagta ccgccgttct tattgtcatt cttagcaaca ggagcggatt
    46981 ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt tgggcaccat
    47041 ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga acaacaataa gatgaacgtg
    47101 aggagcggga cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg aactgaaatg
    47161 attacaattg ccagtgcaat gcaacaagat gaaatcaaag aattgttaga aggatacaat
    47221 gaagaaggaa tgacttttac atttaaagaa aaacaaggaa tcaagctgtt ctttgaaaca
    47281 agtgcagaag atccggaagt tgcggctaaa aaagcaaagg aactaatcaa agcacagcct
    47341 tggggaacag tcctttattt tcaatcaaca gctgtttaga aaatggttta aaaagatagg
    47401 atggttgaga tgataaaaaa cggtttattt ttatgtagta ttggttcttt gatcgtgttg
    47461 tatagtttga atccacattc gcaaaggtat gatttgtata gtatggtcac aggtcttttt
    47521 ctaacaggat taggaatcta tttcttcatg aaggggaaaa aaagagaaga agcaaaggag
    47581 aaaaataact aatggaaatc acgaaaaaat taaatattcc agcatcgttt ttttacgata
    47641 aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac cggcaaatca gtaactagaa
    47701 aacagctgaa aaatttggag tacgtcaaac aattctcgca aaataaccgg gctcgcataa
    47761 aaatcgaaga agtcattgaa aaccagtctt ataagtttcg tacatcgaca acgaagaatg
    47821 aattcgtcgt tcattatcaa atcaaaccat tggacgattc gacctgtgag gttcattaca
    47881 cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa tgatatgctt ttaggaacaa
    47941 tcttgatgta ttttaaacgg agacgtttca aaaaaatgct agatatgatc gaagcaacgt
    48001 attaaatcaa tgaaacatgg tactatttag ttattgaaaa atgaagtaag ctagaaggaa
    48061 tttttcatag gaagatagat ctttcctgcg aatcctcact agcttttttg taggaagggg
    48121 cagcaaaatg acaaacaaag taagagagct aggtgtatct tcgattgagg aaatgtttga
    48181 tttagccact tatgcattta atggggcaga tacacctgaa aggcgagaac gttttcgtac
    48241 acttgcggag aattcttgga attacggatt ttttgatgag gaggatcggc taacaagtca
    48301 agtcatggcg accccttttc cagtgaattt ttacggacaa gagtacctaa tggccggcat
    48361 tggctatgtt gcttcttatc cagaagcaag gggacaaggc gggatcaatc agatcatgga
    48421 aaagatcctt gaagattgcc gtgatcgtaa agtaagctta tcttatctgg cgccattctc
    48481 ctatcccttc tatcggcgct atggctatga acaatcattt gataaaatca gttatcaact
    48541 aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga aaaataaagc gtatggattt
    48601 tgaagatgca aaagctgcaa tcagaagaat ttatgaacag atgccggaaa accaacgagc
    48661 tggtctgaag cgcgaagact ggtggtatac gtataaattc aagcaaaaag gcaattacca
    48721 gtttgctttg cattttgatg ataatgagga agcaacggga tatatcgttt atcagcttgc
    48781 aacgtcttcc tttattattg cggagtgggg atttttggat catgatgctt tttgcagcct
    48841 tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca tttgaatatt catgcggcta
    48901 cggcggtaat gatcaaaatt atctattaga aaatccattt gcttctgtaa tgatcacacc
    48961 ttatatgatg gcgaggatcg tagatatttc tttgtttttg gaaaattatc catttaaaaa
    49021 gagacaagct gcatttgccc tacaaataga agaagatcat tatgcgaaat ggaatgaagg
    49081 aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc tcaaaagtag aacaaacaaa
    49141 actcccaatc gttcgaggaa gtatccaaca tatcacgcag ctattattag ggtatcgtaa
    49201 agtggaagag ttggtattcc aagaaagaat acaggtgaca aaagaatatg aagaaacgtt
    49261 aagctcaatc ttgccacagc aacagccaat tcttaatgac tatttttgat aaaaaacggg
    49321 tgatgacaat atttttgtca ccaccctttt gtttttcatt tggcatttga aggaaaatgt
    49381 taaagtaaat cctctttttt cgtaaatttg aattttttgt tttcttttgt tttagggttt
    49441 tcttttttgt ggtcttggat tgcttctttc gtaatctttt cccgattttt tttagatgaa
    49501 tctaagcgtt ctgcgtatac tttttctttt ggcttatcat tcattggaaa ggacctccct
    49561 acattatttc attctacctc tagtttatca tctgttaagg actagtggaa acgttttgcc
    49621 taacttataa gctgattttt ttagcagata agcttgcaag taaaaaacaa acaagctaca
    49681 attgatttat tgaaattgtt cctgtagctc agtaggatag agcaatcgcc tcctaagcga
    49741 taggtcgctg gttcaattcc agtcaggaac gtacaaataa cccctgtatc ctttgtggta
    49801 caggggtttg ttttgtattt gtgccaaaaa tgtgccaaac tagttatcat ttttatataa
    49861 actatcgaac acagtattga ttttttcttt atcctgatct tcaagctctc gaacaatatg
    49921 aagataagta gacattgtag tttctaaact gctatgtcct aaacgtttcg atacgctaag
    49981 tatatttact ccttgataaa gtaaaataga tgcatgtgta tgccttagtc catgcaaagt
    50041 aaattgcttt tcaatcccta attcttttaa ttttttgcgc aaatatttgc tgacggcgtt
    50101 tgatgaaact aatccatttt ttaagttgaa aaaaacaaaa ttatgagggt ttttaacttc
    50161 aaaattctcg aataattctt tttgattgat tttaaacttt tttaagagat caatcgtatg
    50221 tttatcaatg gaaattttcc tatttgaagt ttcgtttttt gtttttcccc attcatttaa
    50281 tttataattc catgttttat taatagtgat gatttgatct tcgaaatcga tatcttccca
    50341 tgttaatcct agaagttcag caaatcgcat tccagtagca ccagcaacta acacgagcat
    50401 aggagaagag tactgcgcag aaaggttttc ttctacgagt ttcattaaag atttaaattg
    50461 atcataatct aaatatttat cttcttttgg cttcaatgaa tcttttcctt tgattactgc
    50521 ttttctagtt ggatcaaacg gaattaatcc ttcttctacg gcatctttca acgatgctct
    50581 aatatggtta ttaaacttaa taacggtgga ttttacatgg tctttcgcat acttatttaa
    50641 aaagcgttga tatccaactc tatctaaatc agaaatcaat accgctggca tatatttctt
    50701 tatgttcatt aacgtatctt cgtattttct ataagtgata ggagatactg ttccttcttt
    50761 ataaagttgc atccaatcct caaagtaatc tgataataat agatttttgc gatccccttt
    50821 aaggcctttg ttcaagttat attctaattc gttggcggca tcttttgctt cgccttttgt
    50881 cttaaatcct gattttctta tcttcgtgta ttttccgtca tcttttttgt atgaaatttc
    50941 gtattgccaa ctatttccac gtttgactaa tcttgccata attgaatcta ctctctttct
    51001 ctgatacaat aggcactata aagaagccta ctgtataggt ttgtttttct tagaacacgc
    51061 ttgctttgga cggtgggcgt gttttttatt tattatacag ttgctttaaa aatagctgct
    51121 ttttcaaatg atactaaagg agagaagtgg atttctattt ctcccttttg gtttaacgtg
    51181 aaatgagcag ttacatccat ttgttttcct ggagcaacag atcccattgt attttcgttg
    51241 gcatatgtct ctgatttttt gtcatctggt ccatatactt ccacatctgt acctacaggg
    51301 atatctgaat cgccatcatt ttttacggta taagtaattt ttactacttg tgcaggttgg
    51361 ttttcttcaa attgatttct ttcatcagtt agttctacac tatttagtgt atattcagca
    51421 tcaccaactt ttacagtgtc accaatctta tagaaggtat cgcttttttc ttctttagaa
    51481 gatgaagcag aggttgaaga tttagttact ttttcgccac cattatcact tgctttatta
    51541 ctatctgatc caccatttaa agcagaacca ataattataa ttaaaattac tgctaatacc
    51601 caaaaccata cttttttgta aaaaggttta ctaactttat acatttttcc gtcttgaccc
    51661 ataacttttt ttgccattta aatattcctc acttcttgtt ataatatatt tgcgatctca
    51721 gaaatgaggt atgagtccgt gtgccagcac gggctttttt aatgttttgc tgaaatcggt
    51781 ttttttaata attcttgatg tattcataca tattgccttg cgtaagaata ttttttctta
    51841 aaatagcatt ggcagacagc ataacaagtg tatctgagct tattctgaat agaataatat
    51901 tccataaatt tttcgaggtt aaactgagat tcatcagtca gttcgttctc aatatagata
    51961 tttaataaaa ttagaatagc tattttatct gcttctgttt caaattttga gtgaaaagtt
    52021 gtagaagtat cgtacaaaac tgaaaattca aaatgagagg cgctgaaatg tgcaagttca
    52081 tgagataaat gaaaggcttc tgcagtttcg ccatatagat tttcattcaa aaaaatgatt
    52141 ctaggcttcg gataatagaa gccaggctct ttcatttcca tgtaaactac tttcaaattg
    52201 tattcgctca acatttcttt caatttcaaa tacatacaac ccatcactcc aactattcat
    52261 tttcctctaa agctttagca attgcaatcg ctttacgcat tgtctcctta gatatttctt
    52321 ttccatcaaa agaaaaaaca gtatcgtttt ctgataaatc cacatgttta ggggtttctt
    52381 tttcttcgcg ccccagaaga tagtctacag agacatcgaa atagtcagca atttcttgca
    52441 aacgttctgt agaggggttt gagttcttaa gtctatataa gacgttttta ccatagccta
    52501 ggtcttcttc aactttattt aatgattttc cctgtttttt cgctaactct tttattctct
    52561 caaacgttat catatcaata ttcctttcgt tcaagaaaaa atatttaact ttatttgtta
    52621 aaatt
//
LOCUS       DAHUVV010000009        51424 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.009, whole genome shotgun sequence.
ACCESSION   DAHUVV010000009 DAHUVV010000000
VERSION     DAHUVV010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.009"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            609..767
                     /locus_tag="NK767_000526"
     CDS             609..767
                     /locus_tag="NK767_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6693541.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            839..2065
                     /locus_tag="NK767_000527"
     CDS             839..2065
                     /locus_tag="NK767_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6693542.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(2153..2545)
                     /gene="rpsI"
                     /locus_tag="NK767_000528"
     CDS             complement(2153..2545)
                     /gene="rpsI"
                     /locus_tag="NK767_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCH6693543.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2561..3004)
                     /gene="rplM"
                     /locus_tag="NK767_000529"
     CDS             complement(2561..3004)
                     /gene="rplM"
                     /locus_tag="NK767_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCH6693544.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3523..3990)
                     /locus_tag="NK767_000530"
     CDS             complement(3523..3990)
                     /locus_tag="NK767_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCH6693545.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4110..5648)
                     /locus_tag="NK767_000531"
     CDS             complement(4110..5648)
                     /locus_tag="NK767_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCH6693546.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5728..6414
                     /locus_tag="NK767_000532"
     CDS             5728..6414
                     /locus_tag="NK767_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCH6693547.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(6615..7142)
                     /gene="msrA"
                     /locus_tag="NK767_000533"
     CDS             complement(6615..7142)
                     /gene="msrA"
                     /locus_tag="NK767_000533"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCH6693548.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(7226..7903)
                     /gene="pgmB"
                     /locus_tag="NK767_000534"
     CDS             complement(7226..7903)
                     /gene="pgmB"
                     /locus_tag="NK767_000534"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCH6693549.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7900..9030)
                     /locus_tag="NK767_000535"
     CDS             complement(7900..9030)
                     /locus_tag="NK767_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCH6693550.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(9030..11213)
                     /locus_tag="NK767_000536"
     CDS             complement(9030..11213)
                     /locus_tag="NK767_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCH6693551.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11210..12235)
                     /locus_tag="NK767_000537"
     CDS             complement(11210..12235)
                     /locus_tag="NK767_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCH6693552.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(12280..13107)
                     /locus_tag="NK767_000538"
     CDS             complement(12280..13107)
                     /locus_tag="NK767_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCH6693553.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13104..14171)
                     /locus_tag="NK767_000539"
     CDS             complement(13104..14171)
                     /locus_tag="NK767_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCH6693554.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14197..15033)
                     /locus_tag="NK767_000540"
     CDS             complement(14197..15033)
                     /locus_tag="NK767_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6693555.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(15030..15899)
                     /locus_tag="NK767_000541"
     CDS             complement(15030..15899)
                     /locus_tag="NK767_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6693556.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15969..17252)
                     /locus_tag="NK767_000542"
     CDS             complement(15969..17252)
                     /locus_tag="NK767_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCH6693557.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(17266..18951)
                     /locus_tag="NK767_000543"
     CDS             complement(17266..18951)
                     /locus_tag="NK767_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCH6693558.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            19226..20251
                     /locus_tag="NK767_000544"
     CDS             19226..20251
                     /locus_tag="NK767_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6693559.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20846..24520)
                     /gene="rpoC"
                     /locus_tag="NK767_000545"
     CDS             complement(20846..24520)
                     /gene="rpoC"
                     /locus_tag="NK767_000545"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCH6693560.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(24578..28195)
                     /gene="rpoB"
                     /locus_tag="NK767_000546"
     CDS             complement(24578..28195)
                     /gene="rpoB"
                     /locus_tag="NK767_000546"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCH6693561.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28923..29891
                     /locus_tag="NK767_000547"
     CDS             28923..29891
                     /locus_tag="NK767_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCH6693562.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29909..30814
                     /locus_tag="NK767_000548"
     CDS             29909..30814
                     /locus_tag="NK767_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6693563.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30811..31617
                     /locus_tag="NK767_000549"
     CDS             30811..31617
                     /locus_tag="NK767_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6693564.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31753..32742
                     /locus_tag="NK767_000550"
     CDS             31753..32742
                     /locus_tag="NK767_000550"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCH6693565.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33059..33583)
                     /locus_tag="NK767_000551"
     CDS             complement(33059..33583)
                     /locus_tag="NK767_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HCH6693566.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33870..35681
                     /gene="scm"
                     /locus_tag="NK767_000552"
     CDS             33870..35681
                     /gene="scm"
                     /locus_tag="NK767_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCH6693567.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(35836..36822)
                     /locus_tag="NK767_000553"
     CDS             complement(35836..36822)
                     /locus_tag="NK767_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCH6693568.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(36845..37411)
                     /locus_tag="NK767_000554"
     CDS             complement(36845..37411)
                     /locus_tag="NK767_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HCH6693569.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(37425..37841)
                     /locus_tag="NK767_000555"
     CDS             complement(37425..37841)
                     /locus_tag="NK767_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCH6693570.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(38298..40232)
                     /locus_tag="NK767_000556"
     CDS             complement(38298..40232)
                     /locus_tag="NK767_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCH6693571.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            40513..41469
                     /locus_tag="NK767_000557"
     CDS             40513..41469
                     /locus_tag="NK767_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HCH6693572.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            41559..42662
                     /locus_tag="NK767_000558"
     CDS             41559..42662
                     /locus_tag="NK767_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCH6693573.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            42675..43628
                     /gene="menA"
                     /locus_tag="NK767_000559"
     CDS             42675..43628
                     /gene="menA"
                     /locus_tag="NK767_000559"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCH6693574.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(44442..45788)
                     /gene="gor"
                     /locus_tag="NK767_000560"
     CDS             complement(44442..45788)
                     /gene="gor"
                     /locus_tag="NK767_000560"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCH6693575.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(45922..47175)
                     /locus_tag="NK767_000561"
     CDS             complement(45922..47175)
                     /locus_tag="NK767_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCH6693576.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(47181..48104)
                     /locus_tag="NK767_000562"
     CDS             complement(47181..48104)
                     /locus_tag="NK767_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCH6693577.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(48325..50817)
                     /locus_tag="NK767_000563"
     CDS             complement(48325..50817)
                     /locus_tag="NK767_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCH6693578.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(50824..51291)
                     /locus_tag="NK767_000564"
     CDS             complement(50824..51291)
                     /locus_tag="NK767_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCH6693579.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT        14884 a        10841 c         9460 g        16239 t
ORIGIN      
        1 tttagatttt tttctgttgc tgaagattag aatgacaaaa ctatttgaaa ctacgcgtat
       61 agtaagtagc tttcaaaata tataaaaaag gagcgttctc aaatgagaac atgctcctaa
      121 aactacttta ttggcgtatg gaaaaattat acttgaagtg gatagcgggt tgaataagtt
      181 gataagcatc aaaacattta taaaaattat tcaagactat gtctgttcca tatacaaata
      241 tgaaaatcag taaactccat ttaacttttt tcgtagtgtg tctttttata catgtttatc
      301 gttattaata gtgaatagga aaaaaactat ttttgatcat tgacaattaa atacaatttt
      361 tgaataacga tccctgctat tcgagctaga aactcaagta cgccatgacc tgattagcga
      421 caaatactta gagatttctt tttgttctgg gaaaacaaaa tgccatgaca atagcagcaa
      481 ataatgatac ttccgtaagg ctcgccgtaa actggggaga ggtgaaattc ctatgtgctt
      541 acccaaagca attcaaaaat ttcataacta ctattttagg agatgatttt atagaatcca
      601 gagtctacct gaataaaaaa caaacctgtg catttctagg aatcgataat aatactctag
      661 ataagtggat tcgtatatat aatttcccag taataagagt tggtggtgta gttcgctttg
      721 acgtagaaga tattaaagac tttatgaata catttaaaat aacttgaaat tttcttctcg
      781 ccgcatatac taaaattagt gaaatatact gccttacttt ttttaaggag tgaggatttt
      841 gcctacaaag ctttcaaatg ggaaatataa aactaatctg agatatccaa aaaggtttaa
      901 agaaataaca ggaattgcat cagaaaagta tcaaaaaaca tttccaaata ggcaattggc
      961 gattaaagcc gaaaatgata tgaagaagaa aatagaaaaa gttttgagag aggagaatgc
     1021 aaactcatta gaattaaaag gaaaaataac gtttaaaaaa ttttacgaat ctaaatggct
     1081 tcctagatat gaattaggtc aaacaattag atcaaacagg cctcctagtg atataaccat
     1141 tagtaataca aaagatatat ttcggttgca tattttacct atgtttggtg aatacgcaat
     1201 gaattatcta aatatcaata ctgagataat atcagatgaa ttaaccaaaa aatcgaaaga
     1261 atatgcaaat ataaaaatca taaaaggata tgttcgttcc atgttcgata ttgcagaaat
     1321 actaaattat atcgaattta atcgaactac taaaattatt caaagtatca ctgcacctaa
     1381 aaaaaatgct ttagaagaaa aaagaataca agaaggtaag caagcactat catcaaaaga
     1441 acttactaat tggatagaag cagtaaatga tgattttaat aatcatttac ttacttttca
     1501 tgattacaca ctttttatga ttaccctgta tcttggtgat agaaaaagtg agacttacgc
     1561 tttgcaatgg aaatatattg attttgaaaa acaaacggtc cgtcttaaac atactttaga
     1621 taaatatcaa agaaaaaagt ttacaaaagg tcgaaaagat accgtcattc aagtaccaga
     1681 agttgtaatg acattgctaa gtgaatggaa atcggtacaa gctgatcaat tgttgaaatt
     1741 aaaaattaag caaacgttag atcagtatct ctttacttat actaaaccat ctggagaagt
     1801 aaattgtcct gttcatgccg attacctaaa ctatcgaatc aattcaatta aaagaaggca
     1861 tcctgactta gttcatttat ctccacacaa actccgtcat acttatgcaa ctatagctag
     1921 acaaggtgga gcagatatga atcaaatttc aaatgcttta acacactcag atatttctac
     1981 aacaaagata tatgttaaca caccagatat agttgataag gctgtttttg aagcttttca
     2041 gagaggattg aataagtgtg attgatatgt gttcttaaat gtgttcttaa aaacaaaaga
     2101 agcactttcc aaataattga aaagtgccag aaacgttgat atataaagga tattaacgtt
     2161 ttgagaattg tgaagcttta cgtgctttct taagacctgg ttttttacgt tcgaccatac
     2221 gtgcgtcacg agtaagtaag ccagcacgtt taagagctaa acggaagtca ggatcaactt
     2281 ctagcaatgc acgagcgata ccatgacgga tagctcctga ttgtccagcg tatcctccgc
     2341 cgtttacgtt aacaaatacg tcatatgcgc cttttgtttc agtaacagcg aaaggttgat
     2401 tgattacttc acgcaagtca gcatgtggaa tgtattcttc aacatctttt ttatttacag
     2461 taattttgcc agttcctggt actagacgta cgcgagcaac tgcgttttta cgacggcctg
     2521 tgccgatata ttgaacttgt gccaatgaaa ttccctccta ttagattaag tttgtgatgt
     2581 ctaaaacttc tggttgttgt gctgcatgtg gatgttctgc tccaccgtat acgttcaatt
     2641 ttgtaaattg tttgcggcct aaagtgtttt taggaagcat accttttaca gaagtttcga
     2701 tcaaacgacg agaattttta gcacgcaatt cgccagcagt gattgatttc aatcctcctg
     2761 ggtagttgct gtgacggtaa taaattttgt cagtcgcttt tttacctgtt aatttcactt
     2821 tgtctgcatt gatgacgatg acgaaatcgc cagtatccaa atgaggtgtg aaagttggtt
     2881 tatttttacc acgtagtaca gatgcaacaa ctgttgaaag acgtcccaat ggaacatcag
     2941 ttgcgtctac tacataccat ttacgttcta cttcgcctgg tttggccata tatgttgtac
     3001 gcacgttatt ttcctccaat ttaagttctg atgtttgaca tacacttcga gtttccgggg
     3061 ctctttgtgg ggcaaacaat accatttaac atattacccg ctaatctagt caatgtcaac
     3121 gtttttctgc ttgattgaaa aatttatatg aaactttact tcttttcttt cttttatata
     3181 aaagggtcgt ttcctgtcaa aatgaaaaac aaaaagaggc tgtgacaaaa gtcttgtcac
     3241 aaccccttat tttgaataaa cggtgttctc ctgccaactc ctcggtcaca agtcacaata
     3301 tccataaaac ataagaagct gttttttaaa attgtttctt gtgactcgga gctgaacggc
     3361 aggcttacaa cctcttggta aacggtgttc aatcagctga ccctcggtat tagctcaaaa
     3421 aacggcaaaa tatgaggaac tattttgacg tttgtttctc taatactcag gtcaaaacgc
     3481 tgatttcaca acctcttttt cggtcaagtt caacaacagt ttttacgcgt cgatctctgg
     3541 cgctttgccc aaatgagctt ggatcatcca gatacgtttt tctgtggctg ttttgaattc
     3601 gataaagata tcttgtgttg gatcgtcgcc ttcttctcca gaaacttcga ttcctttttg
     3661 gtaaagatct gctagataac gatagcctgc aacaagtgtt tccaaacgtt ctggaatagt
     3721 acgatcccag cggccaattt catcagggat ttttgtgtta tctgcaaatt cacgcaatgt
     3781 agaataaggt gaaccgtcta atgtgatcaa gcgttcggat acttcatcta actgagcgtt
     3841 caaatcatcc atgaactcat ccatcattgg gtgcaatgta aggaaacctg gtcctctcat
     3901 gtaccaatgt gtttggtgaa caacaacaga catttggctt agatctgcaa ctaattgatt
     3961 taatacttct ttggtttgtt ggaatttcat ctttcttgcc tcctaacatt ttcttacact
     4021 taaaataata gtagactttc aatgaaaagt acaacaaaat catttacctg agaacgattt
     4081 tcagttcgct tttttatccg taaagccaat catttttcag aatcagctaa ttcttctcgc
     4141 agttgagcaa attgctgata agtctgtgct aaatgctttt gtgtcgaaac aaataatgga
     4201 tagattttct gatatttttt atgatcagtt tcttgtactc caacttgctc tttcaattct
     4261 atcaattcct gtgcttcact gatactttgg atctttccga tgcttctcca gcctaaaatc
     4321 acagctccta atgcggatgc ttctgtgaca gtagggaaaa caagcgggta acccagtaca
     4381 tcagccatca tctgacgaaa aatcggagat tgagcaaacc cgcctgtagc acggatctct
     4441 gtgagctgat tttcctggca aactcctttt aaaatccgat aaaggttgag acagatccct
     4501 tctagaatcg ccttcatcat ttctttttct gtatgttgcc gctgtaaacc gataaaactg
     4561 ccaaacgcat ctgcgtccca aagcggtgct cgctcaccaa gtaaataggg atgaaaaatc
     4621 agaccattgg cacccgctgg cacaaaagcg atagccgaaa gcagtgcatc atatggattt
     4681 tctccttttt ctttagcaga ttgaacgact tcctgtaaaa atgtatgccc tgcccaatca
     4741 aagatgccag caccattgct agtcgctcct cccactaccc aatgtttctg atccagaaca
     4801 taacaaaacg tctccatttc tggatggaac tgaggctgag tagtaataaa acgaagtgct
     4861 ccgctcgttc ctacggtcaa agcggcgatc gtatcaccaa aagcacctaa ccctagatta
     4921 gaaagcggcc cgtctgcccc tcccaaaaca aatgatatat catttggcaa acctagttct
     4981 gctgctgttt ttgatggtag acctgttact tgataggtgg aaggaaagac ttgcggcaat
     5041 tgctctattc gaatgttcaa gtagtcaagc acttcttctg cccattggaa ttcatggata
     5101 tcaaaatatc cagttcctga agctgttccg tagtcgctga tcagttcgcc aaacaatcta
     5161 taaaagatat agtctttgat accgacaaaa taagcagttt ttttgaataa ttcaggttgg
     5221 ctgttttgca gccacttgat tttgttcaat ggtgtcattg gatgaatcgg cgtcccagtc
     5281 tgccgataaa attctttgcc aagcacggta tatttcagtt tttctgcttc gtccgctgct
     5341 cggttatctg cccaagtgat cattcgagat aacggtgttt tctcttcatc caacaatatc
     5401 aggccatgca tcgcagagga gaaggagaca aattgaatga ttgtgtctga aggcaactgt
     5461 ttaattactt cttttataac ggtgcagaca ccttgaaaga tcaattcctg atcttgttct
     5521 gccatccctt gtgtttcttg gatcaaaggg tagcgaaaat aagcggatgc tgcttcttgg
     5581 gcttcatctg tgaaagcaac tgctttggtt tgtgtggttc cgatatctaa tccgattgaa
     5641 acgttcattt tttcacctca aattttatgt atgtacaaaa atttagttta agcttgcgta
     5701 tcttggtttt gaaaaggggg agatggcgtg ttactttatt ttatcattgg ttgctgtatc
     5761 ggatcatttt tatgtttaac cgcccagcgg attccgttag gtcactctat tatttatcct
     5821 agatctcatt gtgtcaaatg ctgtcgttcg ctttcttggt atgaattgat tccgattctc
     5881 tcgatcatcg tccaacgatt tcgctgccgc tattgcaggt gtcggttgcc cttctactat
     5941 ttgttggcag aagccttatg tggtggtctt tttgcctggt tctttacttt ttcttccgca
     6001 agaaattttt cgacgttcat atggatgctt tcggctttgc tattttcgtt gatggattta
     6061 ttttacttca tggtccattc tcacacgcta ttctgttctt gggcaatttt atggattttt
     6121 tggttgcagg ccggcgtctt ccaatggcaa agtgtgctgt taacgtttat cgtaggcggt
     6181 gtgtgccttc gctatggtca atcctatctc ggagctggcg atactctgtt gatgctttct
     6241 tggagtggtg gcttgtctct agaagagttg cttcagatct tgtttcttgc tagtctgttt
     6301 ggaatatccg ttttttctgt ttactttatc tgtcataaaa aaaagttgga aaagctgcct
     6361 ttcattcctt ttttgagcag tgcgttgctg attgtccttc atcttaggta ttgaaaaaag
     6421 gttgtgacaa acgacttgtc acaacctctg atatatagtg ttctcttacc aaatccttgt
     6481 gacttggagc taggcgacag gttcacaacc tctccactaa atggtgttca atcagctgac
     6541 cctcagtatt agctcaaaat gctggtttta caacctctga tatacggtgt tccataagta
     6601 gctttttcgg cttcttattt cttgacgccc caatgtcgac gaaggaaacc tgcacgtcca
     6661 gaaccttctc gataaagatt gtaatggagc ttgtttttct tgtaataatc ctgatggtaa
     6721 tcttctgcag gataaaattc gcttcttggt tcgatttttg tcacgattgg cttatcgaat
     6781 cgcccgctgt cttgcaatgc ttgttttgaa cgctctgcaa tatttcgttc ttcttcatca
     6841 ttatagtaga tcactggacg ataagaatca ccgcgatcag cgaactgtcc taatgcgtca
     6901 gtcggatcgg tttgctgcca atagatttcc accaactgat cataagaaat aacttctgga
     6961 tcatattcga tctctactgc ttctgtatgc cctgttgtgc ctgttgttac ttgttcataa
     7021 gttggatttg gtacatgccc gccagtataa ccggatgtca cagaaatgat ccctggctgc
     7081 tcttcaaatg gtttgaccat acaccaaaag catccgcctg cgaaaactgc tttttttgtc
     7141 atataaaatc cccctttttt ctcatcatac cttgatttcc aaaaggagga taacgttttg
     7201 cttgtgacag aaaaaacgga aactattata tacgtgatac ggcttctgat aaatgccaaa
     7261 acgaacgtac ttgatcaaaa acggttaaat caggaatcac atattcagca gaaggaagat
     7321 ttatgttttc acctattccc aatacatgca tgttaccagc caaagctgct tgacaaccag
     7381 cttctgaatc ttcaatgacc aagcatgcat ttggcggtac attcaattgc tgtgctcctt
     7441 tgagaaaaac ttctggatca ggttttgctt ttgatacttg cgtgccgtca ataaggacat
     7501 caaaataact tgtcaaattc gttttttcta aaatgaggcg tgcatttttg cttgctgatc
     7561 ccaatccgat cttgatgttt gtttttttca agtactcaag aacagctatt gcaccaggca
     7621 aaacggatgt ttcatccatt ttttgaatcg cttgaagata tagattgttt ttttgctcag
     7681 caaaggctga tttctctttt tctgagaatt tctgttccat ttttccaaat gacagcaatc
     7741 gctctagtga ctcggttctg gatattcctt tcagttgctc gttttctttc tcagtgaatg
     7801 gaatggacag ctggttagct aaaaccagcc acgcttcgta atgataacga gctgtgtcta
     7861 caagaacgcc atctaaatcg aatagtgctg ctttgatttt catcgattac ctccttgaaa
     7921 atgaacgact tgaagtaaag atgcgatgtt tttagctgtg cgatgaagat tatcagtggc
     7981 ttgctgtaaa gcttcttgta aaggcacagc tgcaggaagg atcggaaaaa cggccgaaat
     8041 gccaacatct gctgcttttt cgcttccttt accaacactt ccgcaaatag ctatcacagg
     8101 aacgccctct ggtactcttc tagcaatccc aactggcgct tttcctttta gactttgtcc
     8161 atctagacgt ccttcgccta cgattacgag gtctgcttgc tgtacccgtt tatcgaactc
     8221 cagtaaatcc aatacgtagt cgatgccaga gactattttc cctgaagcaa atgctaacag
     8281 tcctgcaccc attccaccac cagcactact gcccgatgcc tcgattactt gaggggcaaa
     8341 tctcatataa aaatcatgca tggcttggtc agccactgct aattcatttt ctttcaagcc
     8401 tttttgtggt ccgaaaacaa aagaagcgcc ttcttttccg cataaaagat tagtaacatc
     8461 agtaatgaca gtgattttgg ctttaatcag ctcaataggc acgttcgttt tatcaactgt
     8521 agcaatttta cctagatttt ctcccagtgc ttctactttt tgattatctt gatcgtaaaa
     8581 agcataacct agaccttctg ccatgccgat gccgccatcg ttggtactac ttccgccaac
     8641 gccgatgatg atgtgtcgaa caccttgttg caacaattgt cgtatcagtt ctcctactcc
     8701 tcgcgtggat aacctaagtg gctgttgttg gtttagtggg acatctttca atccgacgat
     8761 ttctgccatt tcaaaaatcg cagtttgacc gtctgaagcg tactggattt ctttgttttt
     8821 gccaaatgca ttagtaacga ctgctgtttg ccgcttcaac cttaaactgt ctgctaatga
     8881 tgaaaccgtt ccttctcctc catcgcctat aggtagcaga tcatattcag cctgaacaaa
     8941 aacttcttga aaacctgatt gaatggcttg tgctgcctct aaggctgtca gactttcttt
     9001 gaaagaatct ggcgcaatca gaatacgcat tattttcccc tccgttccaa gatcagttct
     9061 ttttccagct ggtattggtc tccattgaca aaaataggta atgtttcttt cgtctctttt
     9121 tttattacta tttgtttttt tgtcaaaaca aatagcaacc tgtgattttt ccaaataaaa
     9181 ggaaaagaaa ttttttccca tgctgaagga agtcttggag aaatagaaag agttccattc
     9241 gttactgtaa tacctgaaaa accaaatata actgctagcc atagtgctcc taatgaagca
     9301 gcatgaattc cgtcatctga tgaatgcgga tctggtccta aatcaatcag gcacgcttct
     9361 tggaataaat gatacgccca ttccatttct tggatacggc aagcctctat ggcgtgaatc
     9421 gctttactca acgatgagtc gtggattgtt ttgttttcat agtaatgcaa attcttagaa
     9481 acgattggtt gagaaaaata ctcaggtaat aagtataaca gcatgacaag atctgcttgc
     9541 ttgctgactt ggagttcatt tacttcgcta cgggcataat caagtaaaat agactggctg
     9601 ccaggacgtt ctttgtaagt ggtcaaatca ataactggct tactgagata cgtgtcatct
     9661 tgcggaatca gctgttcttt gttaggcact ggcaaatata acttatctaa aaaattttga
     9721 cattctttca aaaaaagcgg atcgccagct tgatatttaa cagctaaggc cacattataa
     9781 tgcgccagat agttcgtata tgcattattg tcgatatatt ctgtgtattc atctggacca
     9841 atgactctgt gtatctctaa atgatcatcg acttttacag ctctgctcag ccaaaaagct
     9901 gctgtttcat gaatgagcgt ttttcccttt gtttctaaaa acgattgatc ctctgttgct
     9961 tggacatact cttgaacggc ataagcaagg tctgcactga tatgatgttc tgcggcagct
    10021 gaagcaactt cttgtcttgt tccggttttg atattcaatg cagcaaattt gggtgtctct
    10081 tcctgaccag tgtgtgcgct ttcccatggg aaaagtgcac cttgatagtg ataatctctt
    10141 gctttttgcc gtgcgccttc taatcgatca taacggtaca gcagcaaatt ctttgctact
    10201 tcaggaaact gatataaaaa gaaaggcagg ataaaaattt ccgtatccca gaaaacatgc
    10261 cctttataac cttcgccagt tagtcccttc gcccctacac taacacttgg atcattgcgg
    10321 ggagtcataa tctgtaaatg gtaagcagcg aagtccatcg ctaactgatc gaaagaagaa
    10381 tcgccttgga tcgttatccg atgctgatgc caaaaatctg tccaataaga catcgactgc
    10441 tctaaaattt ctgtataact aacgcttttc tgggcttggg caatactttt cttaaacggg
    10501 tctgtttgct gaagagaggt agagagcacg atgatcttct ccagcgtaaa ggacgtattt
    10561 tttctgatct tttctgaaaa ggaagcggtg atccggcgat tttttgcttg gaatacacct
    10621 gctttattga atttagctgc aatcccaata tcaattttac tttctgttgt ctggtaggat
    10681 gcactgatca aatcgtcacc aaatactcgc agttcttttt caaccaactg ctgtgtacca
    10741 aaattggttt gctgtccatc gatacccgtt ttcacatcta cttggcagtc ttcggtcaac
    10801 ggcttgatga ccactttcag ggctgcctga tggagattat tttgatcagc aacacgacaa
    10861 gtaatgactt ccactttttt tccttgagca gtttcaatga gcgcatagcg tttaagctct
    10921 cccatttcta aatctagtgc ccgctcgtat gcaagaactt tcatcgtctc aagagaaaaa
    10981 tgttcaccgt cgatttttat ttcccatctg atgacatctg ggagattcac taactcggct
    11041 gattctgctc ctaaaggtcg gttatataca ccagctgcaa acattcccct tacttgattc
    11101 cgatagtctt cctcctggct tcctctgata ccaaaaaatc catttccaat ggtcatctga
    11161 ctcgcatact tattgatata ctctaaatca aactgttttt cttctacttt cataaggtaa
    11221 taacttctcc tgtttctgcc gaacgatata aattttccac cagattctgg atgacatatc
    11281 cttgccagcc atctgcaatc atctgagtct ggtcaccgag cgcccgattc acaaaatgag
    11341 ccatacttag ttcatgtggg tcattaactg gcgtggtctt ttgtaaggaa accagctttc
    11401 catctacatc ttcgaatagt tcgcctggat acagattagc acctgcctga tcgccacaaa
    11461 aagccacatt cagtttgtct tctctgatat tcaaagcaaa cgaagtattc agttggatga
    11521 tcttgccgtt ttctaattcc agtgtgccaa aaattgcatc ttctactgtg aatttgcttg
    11581 gatcccattt cccgaatttc cctgaatttt ttttcgtacc aattttttgg aagctgtacg
    11641 cccgaacgct ttttacttta ggaaatccag taatatacat cgctgtatca agcatgtgaa
    11701 tgccataatc gatcaaaggc ccgcctcctt gaagttcttt gtcgataaac gttccccagc
    11761 ctggaacacc agaccgacga agcgcattcg cttctacgta ataaatatcc ccgagaacat
    11821 caagatgatt cttgatcatt gttgcttcgt cagaaaaacg atgctggaat ccataagcca
    11881 aaatctgccc gtttttatcg gctgtctctg ccatctttct tgcttgatca gctgtcatag
    11941 ctggtggttt ctcgcacatc actgaagcat tagcttcgag ggcagccaat acatgttcga
    12001 agtgaaagcg attgggtgta cagacactca cgatatcggg agaaatctca ttcagcattt
    12061 gtgtaactga tgtatacgca tgagaaaatc ctgtacgtcg gcaaaagtct tctgctcgct
    12121 ggctgactgg atcaaccaca gcggccaatt ctaagacatc tgatcttttt tgataataag
    12181 cagcatgtac tttttctgct acttgtccag cgcctatgat tgctactttc ttcatttatt
    12241 tctccctcct ttgcttattt acttattgtt attctttctc tattctttct ctaaatcatt
    12301 taaatgacgc gctgtttcca gacagttttt caagtattgc aatgattcct gataaatttt
    12361 cacttcattt tctcctcgca aacgacattc atataccata taaccttcat actcattttg
    12421 aagtagcgtt tgaaaatggc gagtaaaatc aattgcgcca gatcctggtt ggtagcgatg
    12481 attatctgct aaatggatat gacccacata ctctttttgc gtagtcaatg cttttgaaat
    12541 atcatcttct tcaatattca tatgataaaa atcagcgatg atctgcacat gtttcaattg
    12601 attagagtca atgtaatgac gcgcatccgc taacaaattg atcatatggt cttggtatcg
    12661 gttcaaaggc tccaaataaa taaccgtacc tgtttcttcg gctactttat ctaaataacg
    12721 aagtgattcg ctgactgctt tatagtctcc ttctttgctt cggggtgaaa ccatcggcgg
    12781 taatcgataa gtgaacattc cccaagctgc tgggataacg attcctttgc ctccaacctc
    12841 gcttagtgca cgcaaaatct cagcaatttg acgtacacca ttaagacgcc gttcttcgat
    12901 aaagtcaccg atccacccat cgtagccgtt acaagctgtc actactggga tccctgtttc
    12961 tttgattgcc tgtttgactg tgtcgatatt ttctattaac aacttaccgt cgatttcgta
    13021 ttcttcaaat ccacagtctt tgataaatct aaatttttct aaaatgtctc ttgggaaaaa
    13081 tgcttgatct tgcgtaccga atttcattgt tctcctcctc ggacttgaac gcccattttg
    13141 atactttctt ctgggtgctg gtctacataa cgcatgtaag cttctgcgct ttctgcaaat
    13201 ggcacaactg gatcaatgat ttcttcacaa tccaagtaac cgttcatcaa taattcccaa
    13261 catgtgtcct cgattcgttt acggttccat cttgggtaat ctgggtttgg ttcactagat
    13321 gctcgagcaa agacgatttt tgcattattg aaatgtgctt cgcgaccaag attaaaccca
    13381 gctggaaaag gtttagcaaa ggcaacataa gaaatgatcc cgccatagcc gatccctctt
    13441 aaagaagctt gtaatgcggc agcactgcca ctcgtttcaa taactgaatc tactccacgt
    13501 ttatctgtca attcctttag acgaaaaccg acatctgctt tcataggatc tagcgtgtaa
    13561 tcggcaccgt atttttctgc taattctcta cgctgcgcta tgggatcgat cccaacgatg
    13621 gtagtagcac ctgctttttt agctaattga atcgcaattt gcccgatagc gcctaaaccg
    13681 aagattgcta cagcatcacc tgctcgaaca tgtgcttccc ggataccacc taaagcgaat
    13741 tgagcaggat cgtaacatac tgcatttttc cattgatcca accgatcaag tttacgtagt
    13801 ttataattat caacaccgtt tgtaatcagc gtttcacgaa tgggtccata agtgcagact
    13861 aactctcctt tttgatactc tgtcaccaaa ctgcccgttt cttcgatcgt accgacaatc
    13921 atatttccta attgaaattc gccaaatacc acaccgcgtt ggcttccttc ttctcttggc
    13981 aagaataggc gccattcttc tgaaaattct tcatcgataa atggacttaa tcctctaaag
    14041 tcgacaattt ctgttcctgg ttttggagta gcgaaaatta ctcggatacg tacttcgttc
    14101 ttttcgatct cacggtcttc gtaatctttg aatccggcta ttcttggttc taatgcaact
    14161 aattttttca tcttttcttt cctctttctt ttctttttat ccttttacgc cgccttcgac
    14221 taaatcacct ttaataaatc gttcagataa cgcatacata atgatgactg gtaaagcggt
    14281 caccaatgaa gcggccatca ttcgtcccca aatgtagtct ggtgtactga ataatgtgtt
    14341 caaaccgatt ggtaaagtaa atttttcgga ggtcgacagg aaaatcgaag caaataaata
    14401 atcattccac gaaaccataa agcaataaac gaagacagaa atgattccag aaatagccaa
    14461 tggtataatg atccgaataa tgatctggat ccggctaaga ccatcgatca ttgctgcttc
    14521 ttcaatatca gtcggaatcg tgtcaaaata actttttagc atgaatatcg cagtaggaag
    14581 tgtttgaacg accatcgtga taatcagtgc tgtattggta tcatacaaac ctagaccgga
    14641 aatgatttta aataatggaa caacgatcaa aataccggaa aacatataaa cggtgtaaaa
    14701 gctagcattg attttcgagc gagctttgaa gcgtagtttt gacaaagcgt atgccccgct
    14761 gatcccaatc actactgcag ttacggaggc tatcaaggag atcactaaac tatttttgaa
    14821 atacatgatg aatggaaaaa tctcagcatt gaaaatatcc tgataatgtt caaaagtcca
    14881 ttctttcgga aaaatagttt gtgtcgttgc aattgcttct tgtgaatcct tgaatgatgt
    14941 catcagcatg atcaaaaatg gaaacaaatt gacagctaat agcaacaaaa tcgtcgcgta
    15001 aaataagatt ttcctgctct ttttattttt catttcaagt ccaccttctt tcgtgagagc
    15061 aagacgaccg ctgcaataat gatgaacaat agaatcgata tagctgctgc ttttccaaga
    15121 tcattgaacg caaaggctgt ctgatacaaa taaacgccta agatattgac tttattcgtc
    15181 agtagataga catctgtaaa catatagaac atccagatag atcgtaaaac aatcacagtt
    15241 gctagtactg gtttgattgc cggcagtgtc acgatcttga acttctgcca agcatttgca
    15301 ccgtccattt cagctgcttc ataaagcgtt ttgtcgactg tttgcaaaat agccaaaaat
    15361 gaaataaacg catacggata gtatctccag atagcaaaca aaacgaccat aaagaagcta
    15421 gcaactggct ggtcgaacca cagcggtgct tcgttgaaaa gctgcaacct atctactaac
    15481 agatcattga cgattccata actattattg aacatatatt tccaagcaaa aatcaaagaa
    15541 atagatggcg taacataact taataaaatc agtgaacgca ccgttttcct aaatctgaac
    15601 ttgcggttaa gcaaaatagc tacggctaat cctaaagctg tactgcctgc tacaactaag
    15661 aatgtgtaag caactgttaa acctaaagaa cgataaaatt cactatcact taaaatttca
    15721 agataattgc ttaatccgat aaaaacggcg tctgagttag gattcagcgg catatcgaaa
    15781 aaactgatct gaatattgga aagcattgga atcagcacta aaacagctaa taaaagcatg
    15841 cttggtgcta aaagtcccat ggctaatttt aagtcagaat gtttccttgg acgattcatt
    15901 gaaacctccc cctgataatc tgccaatagc tatctacttt tttcgaacac cattggcaga
    15961 acttcttgtt atttcgtatt tttttgtgcg tcagctaaag cttgatcgac atcttcgttt
    16021 ccgacagtta cttggttcac tgcttttccg atcacacctg agcttgtgat atctcccatt
    16081 ttcgtgaaat tcttttcccc gactagtcca aagacttgta cttcatcaaa agcactagca
    16141 atctcagatg gcagctcgcc aaaagacttg atgacttcat tttctttgta accatccagt
    16201 tcgactactt gtttattcac tggttgagca ccaccaggag acatcaagac ccacttttcc
    16261 atgtttttcg gttgagataa gaaagctgtg aatgcttcag cagcctcttt ttgtttctcg
    16321 tctagaccag aagaaatcgt caatccagat actgtgccat agacagcttg atctttttgt
    16381 gttgggatag caaaaccgat attttctgag ttccccgctt cataaacaga aggcaaaatg
    16441 taagttgaat agattgccat tggtgctgtt ccattcatga aagcatcttt gatttctgtc
    16501 acatcattag agcctggcat cgtgtattgg gataagtttt gataaaacga caatgcttct
    16561 ctcatctttt ccgtatcgat cgttacctcg ccttcatcat ccaaaacatt tgctccattt
    16621 gacaaagcaa attgagagaa cgcttgctca ctcattgttc cttcagcagt aggcatagca
    16681 attccatatt gtttattctc tttatctgtg aaatgttgag caatttttaa aatatcatcc
    16741 caattttctg gttctgaaaa accagcttct gataattttt ctttgtcata ccaaatacct
    16801 tgaacccagc cgctaattgg tgcagcaata tagctttttc catcttctga acgaacaagg
    16861 tttttggctc cgtcataata attatcttct ccagcttcct cgataacgtt ttggactgcc
    16921 gtctgatcaa tcagttcatc tttatccatc actttagcaa aatcctgact gacttccatt
    16981 acttctggta atttaccgga acgagctaac gtaacgattt ttgtattgaa cgctgattct
    17041 tctaccggca cttgcttgat tttgatgtct ggattttctt tttcaaaatc agcaaccagt
    17101 tcattgatca cacttaatcg ttcctgttcg acagaagagt gcatgaattg aatcgttgtc
    17161 tgttgtgatt cgttagagct ttgactttca ccacaagcag taagaagaaa tccagtagct
    17221 agcagacagg tagtaacaat aatttttttc atttttttcc acctcttatt ttattatttt
    17281 cagccacata tattcatacg gctggaatat caactttcgt tcttgactgc aatggataat
    17341 ttgtccagaa aacaggtcta ggtaatctcc ttttggtaaa agataacctg ctgattgatt
    17401 ggaaaaatta tggataacta agactttctc ttccttcgct tcgcgaataa aactgataaa
    17461 agaatctgct gagtccagta agtaaatcaa actattcgga tggaaggctt tttctttttt
    17521 tcgaatagat agcatctggg tcaattcacc atatactgct gttcttaatg gatctgtctg
    17581 caactctttc tcaatatctg ctaacaaaaa tttttgccga ttgatttttc gattctctcc
    17641 tgttttttct acgcctttgt aatcatttcg acttcccagc acactttgaa tataaattgc
    17701 cggcacgcct ggtaacgtta ccaaaaatga atgagcagct aaaaagcgtt tgcttctcca
    17761 ttccgtcgta tctgttggag aacttaacgc atctagatac gtagcgttta tctcatatgg
    17821 actggtcgtt ccatctgggt ttttcttgta gtttactcgg gtaccctctt tcttcagctg
    17881 ttccactaat tcaaggatcg ttttttccgg cagaatacct cgtaccggat tcaatccgat
    17941 accgtcatgg gaggctaaaa aattaaaaaa agttgttttt ttcgatgcga aatcgatggt
    18001 ttttaaccat tctttcaact tacttgtatt acccgaacga atcgcttgaa gggtcaaagg
    18061 tggtagcgaa aactgataga ccatctgtgc ttcattctcg ccattcccaa aataactgat
    18121 attatctttg tgtggcacat ttgtctcagt gatcaatacc gttcctggag ctgcttcatc
    18181 agcgattgca cggaataatt ggatgatttt atgcgtttct ggcaggtgta tacaagaagt
    18241 tcctagttct ttccataaaa atcccacagc gtccaagcga ataaatgaag cgcctttttc
    18301 taagtaaaac aaaagaatat cgatcatttt caataaaact tctggatttt taaaattcaa
    18361 gtctacttga tcttcgctaa aagtcgtcca tacgtgtttt tctttcccgt ttttgtcaat
    18421 gaacgttgta agtaacgggc tggtccttgg tctagtcacg ctgcttgtat ctgtatccag
    18481 tggcaaatca ataaaaaaat ctttgtacgt atcattcaag tttaaatatt cctgaaacca
    18541 agtactttcg gcagacatat gattgcagac aaagtcgaac attaaccgtg tttcgcttct
    18601 catttcttct atatcttccc atgtgcccaa acggtcgttg attgcctgat aatttacaac
    18661 ggagaaacca tcatcagatg tatatgggaa aaatggcaag aaatgtacaa ctctaaatgt
    18721 atctgccaaa tacttttgat aaaatttctt aaatgtacgc aaagttggtt gctgctcttc
    18781 aaaaaattga tccccataag taatcaagaa aatatcttct tcgtcccaat gcagtttgcc
    18841 tgcctggcct ttcttctgct gataaacact gactttttgt tcaatcatag aggcggtttt
    18901 ctcagacaaa tctgagtcgc gataaatcgc ttgtaaaagt tttgtataca tcggattccc
    18961 ctttcttttt tgggagcgtt cccaacgatt tgatcatatt atggaaaacg cttactgtca
    19021 atagcatttt tttaaataat tttgatatac acaaaaatac aaataagaaa gcttatgtat
    19081 caataattaa tagcttctta aaaaaatatg gtctctcatg atcatgcaag ctattcctaa
    19141 attttcaaaa tcatagacaa aaaagaatgt ctattgtact atggaattat tctaaaacgc
    19201 tcccaaaaag gaggtgcata taaaattgaa acctactatt tatgacattg cgaaaaaagc
    19261 taatgtctca aaatcaactg tctccagagt attaaataac cagccaaata tctctgataa
    19321 agcaaaagca agtgtacttc aagcaatcaa agaattgaat tatgaaccga gcaaaattgc
    19381 aagaggactg tcaggaaatg gttttgacgc gattctagca ttatctcatc gtacttctca
    19441 tacgacctat ggcaatcctt ttttttcaga tgttattcaa tccatttctg ccgtttctga
    19501 acaaaataac ttcgacttga ttctacaaac ggcaaaaaca ccagaagaag agctagaaaa
    19561 atgcgcgaaa aagatccaag aaaaaatgat ccgaggaatt attattttaa gctctacgat
    19621 ggatgaaaca cttctttttg aattggatca atacaatatt cctattgtgg taatcggaaa
    19681 agtcggaaac gactacacga acatctactc agtggacacg gataatttcc aggatagtta
    19741 cgatttggtg gaaacactga tcaagaatgg acaccgtcat atcggctgtc tccatgctcc
    19801 gacagaagtt tatgtatcca atgatcgtct caatggttac cgtcaatgct tatttgatca
    19861 ccacctagat atccgtaatg aatggattat agacggcggc tttcgcttag aagatagcat
    19921 ggcagcaatg aatgatttaa tgaagctacg agaaagacca actgcgattt tttctaccga
    19981 tgcattaaaa tcattgagta tttacaaagt ggcagaacaa tctggaaagc agatacctga
    20041 tgatctttcg atcgcaggat tcagtgatcc cttattgtcc tccctatttt cgccacagtt
    20101 gactcggata gacattccga caaatcagtt aggaatcaaa gccacagaac ttttattcca
    20161 actaatccac caccaatacc ctgaagaaaa aagaatcatt ctttcaaccg agattactct
    20221 cacagattcg atccaacaac aagaaatgta gaacagaatt ataaaagaac agaaacactg
    20281 aggataagtc agttactctg ttcttttttc aatcactcat ccctcgttac cccctcccgg
    20341 tattttagcg aatttttctt attttccatc tgttttagat agctgtggat aactataatg
    20401 tttgtgttaa actatttttt ccacagggaa gaaaaaacta tttttaacca cgttgtgtat
    20461 aagtttttat agttcgtttt tttaaacaca tttaccctta tttcaatcca tgttatttgg
    20521 ggatacttat ctctaaaaca taaatagaat aacagacatg tctactggtt ttgttttatt
    20581 cgaggaatta ttcaataata gctagcaaaa tattaaaaat aaccgctcta tcttcccatc
    20641 aatttatttt gcattcaaaa aatgatctat ctttactttt gttgattgac ttctcttgga
    20701 agatgatcgt tccgatacct taattggata cgaaccgatg ttgttaagat cctttcccac
    20761 ttttcttcta gttagacaaa ttattttaga caaaccaaaa aagaggttgt gacaataatt
    20821 tgtcacaacc tcttttttaa aatatctatt ttgtttgatt gttcattgcg tcttctgcag
    20881 ccatttgcgc ttcaatatct gaaatactgt agacattttc gcttgctaca ccgacttctt
    20941 ttggttccat gtcacgataa cgttgcatac cagtaccagc tgggatgatc ttacctataa
    21001 taacattttc tttcaaacca agaagaggat ctttcttacc gcggatcgca gcatccgtca
    21061 agacacgagt tgtttcttgg aatgatgcag cagataagaa actatttgtt tctaatgatg
    21121 cttttgtgat acctagtaag acaggtcgag aagttgcagg aacgcctcct gagactagcg
    21181 tattgtagtt ttgatcttta aagtctgcga tgtctaataa tgtacctggt aagatatctg
    21241 tatcacctgg atccattaca cgaactttac gcagcatttg acgaaccatt acttcgatat
    21301 gcttgtcgcc gatttctacc ccttgcatac ggtaaacacg ttgtacttca cgaagcaagt
    21361 agttttcaac agataataca tcgcgaactt gcaacaattg ttttggatcg attgatcctt
    21421 ctgtcaatgg tgcaccacgg tggatcatat cgccttctgc tactttcata cgtgctgtat
    21481 aaggaacagt atacgtacgt gtatctgttt ttcctttgat tgttacttct ttggtacgtg
    21541 ttgcaggatc ttctgagata tcgatgacat cccctgttac ttctgtgatc actgcttgcc
    21601 ctttcggatt acgtgcttca aagatttctt ggacacgagg aagaccttga gtgatatcgt
    21661 ctccggcaac cccacctgta tggaatgtac gcatcgtcaa ctgagtacca ggttcaccga
    21721 ttgattgagc ggcgatcgta ccaactgctt ctccgacttc aacgtcagag ccagttgcaa
    21781 ggttgcgtcc gtaacaatgt ttgcagacac catgttttgt attacatgtg aagacagaac
    21841 ggattgtcac tgtttcgaca cctgcatcaa cgatttgttt tgcgatgtct tcggaaatca
    21901 attggtttcc tgcaatgatc aattcatttg tttcaggatg acgtacttct ttacgggtgt
    21961 aacgtcccac taaacgatct tctaatgatt cgatgatttc atttccctcg cggatggctt
    22021 ggatttcaag acctcggtca gtaccgcaat cttcttcacg gatgattaca tcttgggcca
    22081 cgtcaaccag acgacgagtc aagtaacctg aatcggcagt cttcaaggct gtatcggtca
    22141 tccctttacg agccccgtga gttgagataa acatttccaa gacggatagt ccttcacgga
    22201 agttcgaaat gattggcaat tccatgattc gaccattagg agcggccatc aaaccacgca
    22261 ttccggcaag ctgtgtaaag ttggaaatgt taccacgagc tccagaatca gacatcatga
    22321 agattggatt tttcgcttcc aaaccttcca tcagtttttg ttggatgctg tcttttgcag
    22381 cattccaaac agcaatgaca cgttcataac gttcgtcatc tgtgatcaga ccacgacgga
    22441 attgttttgt aatagtttca acttgtttat gggcattttc aatgatttct tgtttttcat
    22501 tcagtacact gatgtcagcg atccctactg tgataccggc atgtgtcgaa tgtttgtacc
    22561 ctaagtcttt catacggtca agcatcttag aagtttctgt tactttgaat cgtttgaaga
    22621 cttctgcgat gatgtttcct aagtttttct tcttgaacgg taatactaat tcttgctctt
    22681 tgatatgtgc aggaatatca gtaccagctt caacaaagta tttgtccggt gtttggacgg
    22741 tcaagttgaa gtctgttggt tcattcagat aagggaattc aggaggcatg atctcgttga
    22801 agatgatctt accaactgtt gtgatcatcg tacgttcttt ttgccattca gtaaatggtt
    22861 tttcacctaa tgagttaggg ttcacaccga tacgtgaatg taaatgaaca tatccgttgc
    22921 gccatgcgat aacggcttcg ttttgatctc tgaagaccat tccttcgcct tcgcgtcctt
    22981 cttcttccat tgtcaagtag tagttaccta gaaccatatc ttgggaagga gtaacaactg
    23041 gtttaccatc tttagggttc aagatgtttt gagcagctaa catcaacata cgtgcttctg
    23101 cttgtgcttc ttcattcaat ggaacgtgga cagccatttg gtctccatcg aaatcggcat
    23161 tataagcttc acatacaagt ggatgcaaac ggattgcgcg tccttgtact aaaactggtt
    23221 cgaatgcttg gataccaagt cggtgaagcg taggtgcccg gttcagcaat actgggtgtt
    23281 ccttgattac gtcttctaaa acatcccata cttcgtcttc ttgacgttcg attttacgtt
    23341 tcgcattttt gatgtttgaa gctagttcgc gttgaacaag ctcacgcatc acgaatggtt
    23401 tgaataattc aatggccatt tcttttggaa gtccacattg gtacatcttc aagaaaggtc
    23461 caacaacgat aacggaacga cctgagtagt cgacacgttt acctagcaag ttttgacgga
    23521 aacgaccttg tttccctttc aacatatgag aaagagattt caatgggcgg ttacctggtc
    23581 cagtaactgg acgtccgcga cgaccgttat cgatcaatgc atctactgct tcttgcagca
    23641 tacgtttttc attttgaacg atgattccag gtgcattcaa gtctaacaaa cgtttcaaac
    23701 ggttattacg gttgattaca cgacggtaaa gatcattcaa gtcacttgtt gcaaaacgtc
    23761 caccttctaa ttgaaccatt gggcgtaaat ctggtggaat aacagggata acgtccataa
    23821 ccatccagct tggttgattt cctgaagcgc ggaatgcttc taaaatatcc aaacggcgga
    23881 ttgcacgtgt acgtttttgt ccttgtgcag atttcaattc ttctttcaat tcggctgctt
    23941 ctgcatctag atcaacatta tcaagtaatt gtttaatcgc ttctgcaccc atagcagctt
    24001 ggaattcttg tccatattgt tcacgttttt cgcgatattc gcgttctgtc aacaattgtt
    24061 ttttctctaa attcgtattt cctggttcga tgactacata tgaagcaaaa taaatgattt
    24121 cttccaaagc gcgtgggctc atgtctaata caagacccat acgtgaagga atccctttaa
    24181 agtaccagat atgtgaaaca ggagctgcta attcaatatg tcccatgcgt tcacggcgaa
    24241 ctttcgaacg agtaacttcc actccgcatc gatcacaaac gattccttta taacggatac
    24301 gtttgtattt tccgcaggca cattcccagt cttttgtagg accaaaaatc cgctcacaga
    24361 acaacccttc gcgttcaggt tttaaggtac gatagttaat cgtctcaggt ttttttactt
    24421 caccgtaaga ccagcttctg attttttcgg gagaagccaa acctatttgc atactttcga
    24481 atttatttac atcgatcaaa aggggttccc tccatttcat ttaatggcaa accgcaagac
    24541 ggctgatctt cgaccgcctt gcagctcata gaccggatta gtctaaatta tcttctgcgg
    24601 tttcaaagtt ttggatgaca tcgtcttttt catcttccac ttgttcagca gctttctttt
    24661 ctaattcttt ggcagtttgc tgttcggcaa attttgtcaa agcatcaaca gtgatcaaat
    24721 cgtcgtcttc atcgtccatg tcgcgaagtt cgatctctgt ttcttcgata tccaagacac
    24781 gcatgtccag acctagtgat tgcaattctt tcaccaatac gcggaatgat tccggtacac
    24841 ctggttttgg aattggttca cccttgacga ttgcttcgta tgttttcaca cgtccgacta
    24901 cgtcatctga tttgtatgtc aagatttctt gtaaagtata agccgcaccg taagcttcca
    24961 gtgcccatac ttccatttca ccaaaacgtt gtccaccaaa ttgagcttta cctcccaatg
    25021 gttgttgtgt aaccaatgag taaggtccga ttgaacgtgc atgcaatttg tcatcgacca
    25081 tgtgagcaag tttgatcatg tacatcacac cgacagacac acgtccatca aatggttcac
    25141 ctgtacgtcc gtcgtacaga acagttttcg catcgctggc cataccggct tcacgaactg
    25201 tttcccatac gtcttcatca cttgcaccat caaatactgg cgttgctacg tggataccta
    25261 actgacgtgc agccataccc aagtgtaatt ccaatacttg cccgatgttc atacgtgaag
    25321 gcacccctaa aggattcagc atgatatcga ttggtgttcc atcaggtagg aatggcatat
    25381 cttcttccgg cataatacgg gatacaaccc ctttatttcc gtgacgaccg gccattttat
    25441 ccccttcatg gattttacgt ttttgaacga tataaacacg aactaacatg tttacgcctg
    25501 gtgacaattc atctccagct tcacgagtaa agattttcac atcgtgaacg ataccgccgc
    25561 cgccatgtgg tacacgtaat gatgtatcgc ggacttcacg agctttttcg ccaaagatcg
    25621 catgcaatag acgttcttct gcagataatt ctgttacccc ttttggcgtg actttcccta
    25681 caagcaaatc gccatcttgg acttctgcac caatacggat gatacccatt tcgtctaaat
    25741 cttttaatgc gtcttccccg acattaggga tttcgcgtgt gatttcttca ggtcctaatt
    25801 ttgtatcgcg ggcttctgat tcatattctt caatatggat agaagtgtat acgtcatctt
    25861 ttaccaaacg acggctcatg atgatcgcat cttcatagtt gtacccttcc caagtcatga
    25921 aaccaactaa aacgttttgg cctagagcca tttcgccttg ttccatagat ggtccgtctg
    25981 ccagagtatc ccctttttcg actttctcac ccaaatgaac gattggacgt tggttgtagc
    26041 ttgttccgga gtttgaacga cggaattttg ttacatcgta tttatctaat gcaccgttgt
    26101 cgcggcgtac gcggatttca gaagcatcaa cgtattcaac gacaccgtca tgtttacata
    26161 acaaagcagc tcctgagtca tgcgctgatt tgtattccat accagtacca acccatggtg
    26221 cttgaggatt gatcaacggc actgcttgac gctgcatgtt ggcacccatc aaggcacggt
    26281 tggagtcatc gttttccaag aaaggaatac atgctgtcgc aacggcaact acttgtttag
    26341 gtgatacgtc catgtagtct actttatcaa tagatacttc taagttttca ctttgagcac
    26401 gagccataac aacgtcttga gcaaatgttc catcttcatt caatgggctg tttgcttggg
    26461 ctacgatgta atggtcttcg atatcagctg tcaagtagtc gatttggtca gtaacacgac
    26521 ctgtttcacg gtctacacga cgataaggtg tttcgataaa tccgaattta tttactttcg
    26581 cataactgga caaactattg atcaacccga tgtttgggcc ttcaggagtt tcgatcggac
    26641 acatacggcc atagtgggag tagtgaacgt cacgaacttc ataaccggcg cggtcacgag
    26701 tcaatccgcc aggtcctaag gctgaaagac gacgtttgtg tgtcaattcg ccaagagggt
    26761 ttgtttgatc catgaactgt gacaactgag aagaaccaaa gaattctttg atacttgcca
    26821 caactggacg gatattgatc aattgttgag gtgtcaatgt ttcagtatct tggatcgaca
    26881 ttctttcacg aaccacacgt tccatacgtg ctagcccgat acggaattgg ttttgtaaaa
    26941 gttcaccgac tgaacggata cgacgatttc ctaagtggtc gatatcatcg acattgccga
    27001 taccttccat caagttcaag aaatagctca ttgaagcaat gatgtcggct ggacgaaccg
    27061 tttttacagc tgcttctgga tagccgtttc cgatcacgtt tacttcacgt tcagggtctc
    27121 ttggtgaaaa tactttgatg acttggacag tcattggatc tgtgaccaca ccgtcttctg
    27181 atggatagta agtcactgaa tttaggccat tatcaatgaa aggagctagt gtttccatga
    27241 cttggtgagt cagaactgtt cctttttcaa cgatgatctc gcctgtttct ggatcaacta
    27301 atgtttcagc taatgtcaga ttcaatagac gtgttttcaa atctaatttt ttgtttactt
    27361 tgtaacgacc aacgtttgct aagtcgtaac gttttggatc gaagaaacga gcattcagca
    27421 agttacgtga gctgtctgct gtttttggtt cgcctggacg caaacgttcg taaacgtctt
    27481 tcaaaccttc ttctgttctt gagtcgcttg cgtttttatg caagtctttt tcgactgtat
    27541 tgcgtaatgt ttcgctgtcg ccgaagattt caaagatcgt atcatcggaa ccaaatccta
    27601 acgcacgaac taatacggtt aaaggaatct tacgtgtacg gtcgatacgt acataagaga
    27661 tgtcttttgc atcagtttcc atttctagcc aagctccacg gttagggata acagtagaac
    27721 cgaatccttc ttttccgttt ttgtcgactt ttccatggaa atagacacct ggtgaacgga
    27781 ctaactgaga aacgattact cgctctgccc cgttgatgat gaatgttcct tgttctgtca
    27841 ttaatgggaa atcaccgaag aatacttctt gtgctttgat ttcgccagtc tcgcggtttg
    27901 tcaaacgcaa tgttacatgt aatggtgcag agtagtttgc atcatgcgca cgagcttctg
    27961 ctactgtata ctttggttct ttcaattcat aatctacaaa ttccaaagat agattgccat
    28021 tgaagtcatc gattggcaaa atgtcttcaa acatttctct aagtccctca tctaagaacc
    28081 attggtaaga gtctgtttga atttcgatca aatttggtaa ttccaacact tcactgattc
    28141 ttgcgaaact tctacgttcg cgatgttttc cgtattttac tacgtgtcca gccaagctct
    28201 tcacccctct aaaatgtcat tcaaaaagac tacccgatca attgttacaa taatatttca
    28261 aatattattt ttaggtaaca acagacattt tttgcggctt ttaggcaaaa aaaaaccaaa
    28321 aatagaacat tctcttgaaa ccttacttcg tgagcttcta cttttgtttc cttatattaa
    28381 agccgaaacg gacaatattt cgtttctgcc ttttcttcat cagatagttt ttgcaacttc
    28441 atatactaca cttattttct ataaaagtca acacttcctg gcgtttcttc tgctttttcg
    28501 caacattttc ttaatgtttt caattttatg ttttcaatta tctgacaatt cttaattgac
    28561 agaccttttt atctcggtta tactttagaa aattcatttt gtggaggaac ttctatgcga
    28621 accaacatca accaaatcgc accaacacaa ctgaataact tttactttag ctactttaga
    28681 taggtttgta ttcatcatga tggatacaaa cgttaaacga cagtttgtat ctatgatggc
    28741 taaagaactt tgtattgcag caaaaagcca cgtagacaaa atgctaagtg gctgagcagt
    28801 gaaaatacaa agtaaaagta tcaagcattc tactttttca atttattttc cgacttttca
    28861 atccatttgg acattttgtc tgaatggatt ttttgtttcc caaaattaag gaggaaaaag
    28921 aaatgaaaaa aaatataatc atcggattag gcgctgccct gctactagct ttaggaggtt
    28981 gcgcacaaaa agacaattcc gcagaggcat cagatggaca aaaaacaatt ggcgtattgc
    29041 aacttgtaca acataactcg ttagatgctg cctataaagg attcaaagaa ggattagcag
    29101 aagaagggta taaagaaggc gataatctaa caatcgatta tcaaaatgcc caaaacaatc
    29161 aagacaacct gaaaagtatg agtgagaaac tcgtcaaaga agatcctgat ttgctacttg
    29221 gaatcgccac tccagcagca caatctttat taaatgaaac aaccactatc ccgatgactg
    29281 tcacagcggt cacagacttg gaacaagccc atttagtcac atccaatgaa aaacctggcg
    29341 gcaatgtgac tggcacgaca gatatggtgc caatcgaaaa acaaatcggc ttactgttaa
    29401 atatcgtgcc agacgccaaa acaatcggca tcatgtataa caacggtgaa gcaaactcta
    29461 agatccaagc tgatttggca gaaaaagaat tgaaaaaagc gggcgtcaaa gtcaaagtac
    29521 tgacagcaaa ttccacaaat gatgtccaac aagtgacgaa aagtctagct aaagatgtag
    29581 atggaatcta tattcctact gataacacgt tcgcaagtgc ggcaagtgtt gtcggtgaag
    29641 tcgcaaaaga aacaaaaaca cctgtcgttg ctggcgcagt tgatcaagtc aaagacggcg
    29701 gtctagcaac agtaggaatc gactacgaaa gcttgggtaa acaaacaggg atcatggcag
    29761 caaaaatctt agacggcaag aatccaagtg aacttcctgt cgaaacagcg aatgatttgg
    29821 aactgtatgt caataaagac atggccaaag cattaggcat caatccagac agcatcaaag
    29881 aagctgaata aaacacagga ggattctcat gacaaatttg ctctctcttt tcttatcatc
    29941 tgcatcgcaa ggggtcttat gggccctact tgcgattggt gtatacgtca ctttccgtat
    30001 cttggacatt gccgacttga ctgctgaagg aagctttcct ttaggtgcag ggatcgcggc
    30061 agtaagtatt acaaatggct atcatccatt gacagcttgt cttctaggct ttttaggggg
    30121 tgcggcagca gggcttgttt ccggactgct tcacacaaaa ttgaaaattc ccgcccttct
    30181 tgcagggatc atcaccatga ctggtttata ttctgtcaca tcccgtgtga tgggtgcgcc
    30241 gaacatctct ttattgggtc agccaacgat ctttcattgg gtcgagtcat tcggcataac
    30301 aaaagacaac gctgtactga tcgtcggtct gctttttgct ttgatcgttg tatgtttact
    30361 tgttttgttc ttacgtacag aaacaggact tgctattcgt gcaactggcg ataatctggc
    30421 aatgagcgaa gcaaacggga tcaacacaga taatatgaag atcatcggtt atatgatttc
    30481 taatggatgc attgctcttt ctggctcctt gcttgcgcaa aacaacggct ttgctgattt
    30541 aaattctggt attggaacga tcgtaattgg acttgcttct attatcatcg cagaagtttt
    30601 gttacgtaac cagccactat ttctccgtct cttgactgtc gttttaggtg cggtcattta
    30661 tcgttttatt ttagcgttag tcttcgaact gaatgttgaa ccaagtgatt cgaaacttgc
    30721 ttctgctttc gtgctagtga tctgtctttc tttcccgcag atccagcaaa aattccattt
    30781 acctcaaaaa aagaaaggcg gtagtgtcaa atgaatgacg tcttaacatt ggaaaatctc
    30841 catcatacct ttgaaaaagg aacgatcaac gaaaatcatg tcctgaaagg cattaatctt
    30901 tccttagatc ccggcgactt cgtgacgatc atcggtggaa atggggctgg taaatctact
    30961 ttgctgaaca gcatcgccgg tacattttct gtcgatcagg gtcaaatttt attgaatgga
    31021 aaaaatatca cgaaaaaatc tgttgtcgaa cgttcgaaat ccatcagtcg cgtattccaa
    31081 gatcctaaac tagggacagc cgttcgttta acggtagaag aaaatcttgc tcttgctatg
    31141 aaacgaggaa aaaaacgcgg cttcttccgt ggtgtcaaac cacaagaccg aacatttttc
    31201 aaagaccagc tggttcggct aaatttgggg ttggaaaatc gtttatcctc agaaatcggt
    31261 ttgctgtctg gaggacaacg tcaagctatt acgttattga tggccacatt acagcgtcca
    31321 gaactgatct tattagatga acacactgca gcgcttgacc ctgctacttc caaaacggtg
    31381 atggaattga cagaaaagct gatcactgag cagaatctca ctgcttttat ggtcactcat
    31441 gacatggaag acgcgatccg ctatgggagt cgactgatca tgctgcatca aggaaggatc
    31501 gtcgtagata tccagcaaga ggaaaaacag cagctcacag tgccccaatt attagcgttg
    31561 ttcaaacaaa acagcggcac ggcattaaaa gacgatcaag tacttttatc ttcataatca
    31621 gaaagaggat gggacaaaaa atgaacgccc accctcttct tttttctctt tccttctttc
    31681 gtttttgctt tatgtatcaa ccgttgtttc ttcgaaaact attttgttct ctccttcttc
    31741 ctgatagaat ggatgggaaa ggagcttgat gccatgacaa cgaaagacaa ggtacttcaa
    31801 ttattgaaaa agacaccaga ttttctttcc ggagaaaagt tggcacagca actggaagtt
    31861 tcccggacaa gtatttggaa agccatcaaa gaattggaaa aggcaggcta tcgttttgag
    31921 cacgggccta cagggtatcg ttacctgccc tctgacgtac tagatgcaaa agaaatccag
    31981 caagagctac ggtcgtttat tcctgatttg aaagtacaaa cgatgttgac ttctgaatct
    32041 acgatgaaag atgccaaact ggcagcagct caaggagatt ctgtttcgaa gttatttgtt
    32101 gctgatacac aagaacaagc tcgtggacgt tttggacggc cttttttcgc tgaacctgga
    32161 cgaggaatct acatgagtct cttgctccat ccagacaaac ggtttgatga attgcctcaa
    32221 tatacgatca tcgcagccag tgcctgtgtg aaagccattg aatctgtaac cggtcgttct
    32281 gcttcgatca aatgggtcaa tgatatctac atggatggaa aaaaagtctg tgggatttta
    32341 tctgaagcag tcagtgatat ggaaagcggc aggatcagtc atatcatcat tgggatcggg
    32401 cttaattttt ctgttcctca aaatcatttt ccagctgatc tccaagaaaa agccatctct
    32461 gtctttcccg acggacaagc tagtgtcaca aggaatcagc taatcagcaa aatctggcaa
    32521 gaattgttca cttatctttc tcacccagaa aaagcgttgg acttctataa agaaaaatca
    32581 ttcgtattgg gaaaaaccgt gtcgttcaat caaaaaggaa cgacttatgt cggtcttgca
    32641 aaagaaatcg gtgatcatgg cgaactgatc gtccaaacaa atgaacagaa gatgagtttg
    32701 tcttctggcg aaatcagtct cacatctatc cgtacgcaat aattatcaga tttcacaaaa
    32761 ggacacctga tctcatacag tgtccttttt tctgaattct cttcaaaaga ctctccttat
    32821 tctatcagct gacagtttct ctatcctcta gaaaaaggta ttctaaaaat atctataaag
    32881 catggtacac tttctttaaa tgaatcaatg caaaaaacaa gagagtcatc agtttacacg
    32941 atcaaaagag atagcaggcc gcttcctttt tcaatcttat agatagacca tgggtaacga
    33001 taaaaacaaa aaagaacttg cgacttattt tgccgtaagt tcttttttag ctagtgcctt
    33061 aatctcttaa tcccaacaaa cgtctcgcat agatcacctg accagagtgc atagcagcat
    33121 catcaataat agaaacgaga cgcattcctc gtgtgactgg tggtgtccag ttctcgtcaa
    33181 ttacttcatc tagctcggaa ggttccagcc ttcttaagta agaaatagca aaatcagcag
    33241 ctcctcgcaa gtatcctagc agatcttcta tttgattagc ttggatctgc tttgcttgtt
    33301 ccagactatg cttccaatct tgttcgttat ctgctatcgt catcggaaac cggctttccc
    33361 agtcttgact atgccaaatt ggagaagttc ctgctaaatc agcaatctgg aaatctaatt
    33421 ctcgagcggt atgccaagcc agccaagtca ttgatttgat ctgcggggct ctatcttcca
    33481 ctggaaaacg attggcctcc tcaatagaga cgccttcaaa caaacgttca aaccgatcgt
    33541 atgcccgatc cacactctcg catagcatat ctaaataatc catttatttt cctccttttt
    33601 atttatccta cttacttttt cctaattcta ggatacctac ttatatgtta ctacacaccc
    33661 ttatttttta cactttaaca gtcaccatca tcaatagttt ggagattgaa agaaaaagat
    33721 agtgtcgaac tatcaattga acaagaagca acaatatgaa cgtttcatta atattcttta
    33781 aataaacaat gatttttcaa acgaacagtt taagactagg agaaaaagag tataatagga
    33841 aaatgtttga aaggaaagga aggggaaaaa tgaaaaaaag ggcaagttta ctagtcctag
    33901 ttgctttgtt gttcaatatc ttttcgtatg gtgtggccgt ttcagcggaa acatacattc
    33961 ctaaagaccc tgggaatatt tataatgatc taactggtaa ctatggcttg ttaggaattg
    34021 ctagtcaatt ccacgtattt gctaaaaata agacaactgt gaatgcacat acagacggaa
    34081 atatagcaac acatgaactt gatgcaaaaa ataattttgg tacagagctt catgaaggtt
    34141 tattgaacca agaaataaat tatatacaaa aaactgattc tttcgctaat tcttcaggca
    34201 tacctacagg tagcacaaga atgaataaat ttgtcgttgg cagtaacgtg gatgtagcac
    34261 ttcaagatgg tcatcctaca gtcaatggat ccaggcttga tcacttaact ccagatgatt
    34321 tttataaaga tcgtgctggg catgaatata ttgatttcga gcaggaattt gctaaattga
    34381 atgtagcagc aaatgatctt gcaacgatca ctccagctaa aacatatact gctgcagatt
    34441 ttccagacat gaacaatcgg acaatcgatt taactgattt atcagatacc gggtttttat
    34501 tggtaaatat cgatgcagaa gtgttaacga tgaatacgcc acttcaaatc attaatccaa
    34561 atgatcaagt agttgtattt aatgtcatca acagttcttc tgcattgaac gtacagtcac
    34621 caatcaaata caatgatcgt tcaaatcatg aaacagaaga tttttcggat gcgaatatct
    34681 cttggaattt tggaaatgag atgactgatt tgacaatcag tgctcctttt caaggaacaa
    34741 tccttgcgcc taatgctact atccgcatta ctcaaaacca agacggaacg attataggaa
    34801 caaatgtgat tttagatgca gctaccaatc gttgggatcc taacgaaata tttatcagcg
    34861 acaatggtac agatacgaca gacacaagcg acagtacaga tacatctacc acaagtgata
    34921 gctcagattc atcaacttca agcgacagta cagatactac ctctaccaca agtgatagca
    34981 cggatacatc agcttcaagc gacagtacag atactacctc taccacaagt gatagcacgg
    35041 acacatcagc ttcaagtgac agtacagata ctacctctac cacaagtgac agcacggaca
    35101 cgtcagcttc aagcgacagt acagatacta cctctaccac aagtgatagc acggacacat
    35161 cagcttcaag tgacagtaca gatactacct ctaccacaag tgatagcacg gacacgtcag
    35221 cttcaagcga cagtacggat acatctacca caacaagtga tagcacggac acgtcagctt
    35281 caagtgacag tacagatact acctctacca caagtgatag ctcagattca tctaccacta
    35341 caagtgatag cacggacacg tcagcttcaa gtgacagtac agatacatcc tctaccacaa
    35401 gtgatagctc agattcatca acttcaagcg atagtacaga cacgtcgtct accacaagcg
    35461 atacacgtca tgcaagtgtc tccttctcaa aaacaagttc ggataacagg acgaacttta
    35521 acttaactaa taaaacaagc aatgacaatg gaaacaaatc aaaaagagca cttcctaaaa
    35581 ctggaagtca atcgaataat tggataacac tcgcaggcgt tatattactt gtaatcggtt
    35641 taagaatcac ttttatgagt tataagaaat cgaaaaggta aaaaaatatt caagataaaa
    35701 aagagcaacg tcgctaacgg acgttgctct ttatttttgc tttttatcat aaatacaaga
    35761 actacaattc aatttgtcaa aaaacacgta gatttccttc tgagaaattt atttacgtat
    35821 cgaacctttc cctattcagt ctttgcgatc caaaatcagt tgggtaaggg aaaagagatt
    35881 ctcttttgtt cgaagagagg tggcaggtaa tttctttatt tccttcaaag ccttttctgt
    35941 gtaagaggcc gctaacttcc tagcttcttc cacgcctcct gtatgttcaa cgattttgcg
    36001 tacggcgtcg acatcttctt ctgtcattgc cgctttcttc tctaaataag gtagtagctt
    36061 tggtttgttt ttttgcaaag agtagatcaa cggtaaagaa tagacccctt gacgcatgtc
    36121 ttctagtaca ggtttgccga tttcctggct ctcttttgta taatctaaaa tatcatcgat
    36181 aatctgaaag gccatcccga tagaaagtcc gatatctcct gctttttttg cgaagcgctc
    36241 gcttgttcca ctttcaaaag cgccaacaga acaacttaat tggaacagtt cagctgtctt
    36301 acctgaaata ttttccaaat attcttttac agaaagttca taattatagc gatcatccat
    36361 ctgccctaat tcgccattca atactttttc catactgcga gaattcaatt ggagactttt
    36421 caaggaagtg gagtaatcag acaatagttt aaaacagcag acaaataaat agtctcctgc
    36481 atagacagaa acgacattac caaactgact acggatatta ggctgaccac gtcgcagatc
    36541 cgcttcatca acgatatcat catggatcag tgtggccgta tggagcattt cgatagaagc
    36601 tgccaaagcg atggctttgt ctcgatcttg ctctggtcca aattgtgaga acaatagctg
    36661 ataggcagga cgcaacagct ttccgccggc atggatcgtt tctttgattg cttgttcgac
    36721 aggtttgttt ttcaggcgga cagtttcttc catccgtttt agtgtttctg tcaattcttt
    36781 acttaaagca ggatagtttt cccacattgg atgtagcttc ataaaaaaac tccttgtgtg
    36841 acgattattt tttggtaaac attgggctcc atttttgttt ggttccctgc tcataatctt
    36901 tttggaatcg ttgaagtgtc tgcacatgtc gtaaaagata attcgctgcg atcccgattg
    36961 caatccctga taaaatccct aaaactgaaa ggactggcaa gtacaacatg actgtccatg
    37021 attgtgcaat gaaagaagcc atcaaaagct gtccgacatt atgcatgaaa ccgccggccg
    37081 cactaattcc gatgatactg acacgttttg gtcctaattg cttgaccaat aacattccaa
    37141 aataactaag caaagcacca ctcatactat aaaggaatgt ggaaatcgtt ccgcctagca
    37201 aagtagtcag gaatagtcgt agccaaagta gtaagaagct gtctcgtttt ttcatggtaa
    37261 aaatggcaac gatcgtgatt agattggcta agcctagttt tgcaccaggc gcaaaagaaa
    37321 aaggataagg gatcatattt tccaataaac caatgatcac tccttgtgca acaagtagcg
    37381 aaatataaat cattttttgt aatttactca tggtgtgtga aggtctaata aatcagactg
    37441 tcttcgtcac ctccatctgt tgaacggact tcgatcacta atttatgtgg caaacagacg
    37501 atggtttcgc catttttcgt tgcccagcct cgacgtacgc agatctggtc atcgcaatct
    37561 gcttctttta tccgtactcg atcgccatct atctcgatca agttataatc tccgtgttca
    37621 tctttgtaag tatatgtgtt gctctcttct ccagccatca aagggaatgt tttgatttct
    37681 gtgccatcca cacgcagtac cgcttctttt tctgctgttg cggtgttttg ctgaagacca
    37741 aaaatcatga ctggtaaaaa agaaagaaca gtcaaaagca aaataataaa aacatcccaa
    37801 ggtttgagca gacttttttc agcaaattct ttaaggctca tttttgttct cctttttcca
    37861 gattaactca cccaatttta gcataaaaac aagaggttgt gacacctgtg tcttgctttg
    37921 agtaataaga acgaagaata gaaaacagct ttccatgttt tctaattctt tgggcttatt
    37981 accgagaagc agcaggtgga acaccgttta taacaagagg ttgtgacaga agtgggcaac
    38041 ttcaagaaat aagaaggaat ttacgaaaat tacttttcaa gtttttgtga aatttcggct
    38101 tatttttcga agaagctact tctggaacac cgtttataac aagaggttgt aacagaggtt
    38161 gtgacacctg tgtcttgctc atggaggctt atctcgggca tcttattcct ttaattttac
    38221 ataaaaagga ggctgtgacg gtttgtctca gcccctttct attgaaatct tgcattcaaa
    38281 taacgttctt tatctattta tttcttgccg tactgggaac ttggtctacc gtaccaccaa
    38341 ttacctagtg tacgttgtag ccatggaatt acccagcgat ccaacccaat cgcacgtccg
    38401 gaaccgttca tcaatgcgaa agcaacaaaa atgaaccaaa tgtttaccca gtaaaacatg
    38461 cctgataagc agaacgtgat caccaataca atcgttgtcg cactgcatag ccatgtgaat
    38521 aatccagcaa tcaatgctaa tgccaaggca atttcaaaga ataccatgaa tttttggaag
    38581 aacattgcta catcattgtt tggcatgatc acttgcatga tctttgtgaa ccagtctggt
    38641 gacttctcaa tcacagccat tggttcttca ccgtatgcat aactcaaacc aaaatgtgca
    38701 gcttgagtcg ctgcttctcc ggcacttgcc cctgctccgc tggcagctgt ggttgcatca
    38761 ccagcagcag aagcaccact ggcaacatct gttacattgt attcccatgt ccaagggaac
    38821 acactgtttc cagtgaacca tgatccttca ccaaaccaag tacttggttt gaacacttca
    38881 ccattcccaa tgattttttt ggaagcttca acgaaccaaa ctaaaccata gaaaatacga
    38941 agtggcacac tccataagac attgccgtaa cgagaagaat gtcctcgagt cacgttgcga
    39001 tcatctttga tatggaaaaa ctcatgcatt aaatattgga acatataata accagaacga
    39061 atatcaaaga agtatttcaa attcacgata tgtttcatta cgatagctaa aaatccgctt
    39121 aaatggatct tatcaaatag gttagctacc ccccatttgg caccaataga aaccataaat
    39181 ccttgatagt ttcctttaaa tttatgcttt tctccgcctt tgatcgaagc cacgatattc
    39241 gctgcggctg tgtgtcctgt ttgttcagca gcttgaacga tctgcggtgt cggtgttcct
    39301 gggaattctt cataaaagac taaatcacca atgatataga tgtttttatc ttcgtagcct
    39361 tttgcctgca tatattcatt ggcgatcaac cgctgtccgc gtgctgattc taatccaaag
    39421 tcagcagcgt cagaagttcc tttgacacca gctgtccaaa tcagtgtgtg agtcggaatg
    39481 ttgcgtccgt ctttcaattt gatatgatca gcagccactt ctacgattgg tgcattcaat
    39541 aataattcca cgttgtgctt acgcaaatag cgttcagctt ttgcagcatc atttcgatcc
    39601 aacatattca agatcgtcgg cattgcctct acgaccatca gtgtgatttc gtttggatct
    39661 aatttataat ctttggctag gcgatttttc cagtcgatca gttccccaat catttcaata
    39721 ccggtaaatc cagaaccaca gacaacgaaa gtcagcatgg ctttacgcag ttcatcatct
    39781 ggttccaatg cagcttttgc tactgtcttc tcaatatgtt cccgaatcct tacagaatct
    39841 tcaaatgacc aaagggtgaa tccgtgtgct ttgacaccag gtgtaccaaa gtcattcggc
    39901 tctccgccca ttccaaggat cagctgatca aaaggatagg aacctaattt ggtcttgacg
    39961 atttttcgtt ctttatcgat accgattaca gtgtctgtga caagttgtac attcttttga
    40021 cgagcaaaca aatgctgcag atcgtattgg attgcttctg gttctacacg tccgccagct
    40081 acttcatgca actcggtcat catagtatgg tacgaatgac gatcgatcag agtaattgct
    40141 acatcttctt ttttcagttt ctttgccaag tattttgtag ctgaaacacc ggcatatcct
    40201 gctcctacaa cgacaatatt ttgctttgcc attgatcatc cgctccttat agactatcta
    40261 tataatgaat atatgataat tcttttagtt tgatacttat tacacaatct ccatatatta
    40321 tatagatgtt ctgttctttt gtctaactcc ttttggattt ttttcaaatt ttaaaataaa
    40381 gcgttttttc acgaaatttg ctgaaagaaa acgtttgaaa tattacatgg gttatttatc
    40441 aaaaatagtt gacttatagg ctaaaaagat tagaatgtat aatgaatcac aaagaaaaat
    40501 tgtggactag aaatggaatg gtggggtatt aaaatgaaga aaaaacgttt tgtaactgga
    40561 tttgccgtat tagcattttc agctctagtg ttaggcgctt gcggcgcaga tgacaacgga
    40621 agctcaaact cttcttctga atcatctaca gcacaatctt cgactgcaaa aagcacaaca
    40681 gaatcatctg caaaagttgt agctggcgga gatttgcaag acggcacata taaattagaa
    40741 gaaaaaaatt attcaaatgg ttaccgtgca gtctttgaaa tggttgtaaa agacggcaaa
    40801 atcactgaat caaaatatga taacgtcaat gaaaacggcg aatcaaaaac aaaagatgca
    40861 gaatacaaca aaaacatgga agcaaaatct ggcacaaacc cagaaaaata cattcctgag
    40921 ttgaatgacc aattcttaaa agcacaaagc gcaagcggtg tggaagtcgt aactggagct
    40981 actcactcat ctgaaagctt ccaaaactac gcacaacaat tgatccaagc tgctcaagct
    41041 ggtaacacag atacaatcga aatcgacaat ggtgctgatt tgaaagacgg cacttacaag
    41101 cttgaagaaa agaactactc taacggctac cgtgtgcaat tcgaaatgac agttgctggc
    41161 ggcaaagtaa ctgaatcaaa ctttgattat atcgacaaag acggtaaatc aaaacaagat
    41221 gatacagaat ataatgaaaa tatgaaagca aaaagcggta cagaaccaaa aacttacatc
    41281 cctactctga acgacgaatt cgtcaaagca atgggcgaag aagatggctc acctgctaat
    41341 gtagaagttg ttacaggtgc tacacacagc tcacattcat tcatcatgta cgcacaacaa
    41401 ttagtgaatg cagctgaaaa aggcgacact caaacaatcg aagtagataa tatcgtaaca
    41461 gaaaaataat cacataatca tactaataaa agttggaggg tgggacgata gccagattag
    41521 gcagtcgtct cacctttcaa aatgaagaga ggaaaaagat gcggaaaaaa ctactatttt
    41581 ccttaatcac actgggcatc tcttttatgg tgctaactgg ctgcagcaac tcagcaaata
    41641 aaacaaccac tgaaagttca gcagctacca agatattgaa agatccatat tctgatgaac
    41701 agttcttgtt agggacttac gttcgtatcc gtgtgtatga tgaaggcaaa gaatcagcat
    41761 tgaaacctgc cttcgatcga gtaaaagaac ttggtgataa aatcacgatc aatcaaaaag
    41821 gttctgagat cgatgaagtg aatgagcaag ctgggatcaa accagtaaaa gtttctgatg
    41881 acgtctacac gttagtcaag cgggcctacg aatatagcca agattctcaa ggcggattcg
    41941 atatggcgat tggtgcaatc acacaattat ggcgaatcgg ttttgatgac gccagaaaac
    42001 cttcccaaga agaaatcgat caggcattga aattagtgga ttatcataag atcgaactaa
    42061 atgacaaaga aaaaactgtt tatctaaaag aaaaaggaat gatcatcgat ttaggtgcaa
    42121 tcgccaaagg ttacattacg gacgaagtag tgaaagtctt gaaaaaacaa ggagtgacca
    42181 ctgccatcgt tgacttgggc ggtaatgtct atgtcttagg ccacagccca agaggcgaaa
    42241 accaagactg gacagtcggc atccaagatc cgaacatggc tcgcggatct gttttaggtt
    42301 ctattaaaga acggaacaaa acattagtta cctctggaat ttacgagcgt tatttagaag
    42361 tagatggcaa aaaataccat catttgtttg atccaaaaac gggttatccg ttcgacaatg
    42421 atatcgcaag tgtcacgatc ataacagata aatcaattga tggtgacggg ttgtctacag
    42481 cagtcttttc aatgggtgtg aaaaaaggac tggaatacgt cgaaagcgaa ttgaataacg
    42541 ggacggaagc gatcttcgtg acaaaagacg ataaagtata tgtgaccgat ccaatcaaag
    42601 acaccttcaa attatcggag gactcccatt acacgatggg tgaccgttct gaactaaaat
    42661 aaggagtacg aaccatgaca ctaaaaatct ttttagaagt agttgaaata cgaacaaagg
    42721 tggctagtgt atttcctttt gcggtagggg tattattctc tatcgctttt tttcatgaga
    42781 tacattggct aaacactttt ttatttttta ttggaatgct agtttttgat ttagcgacta
    42841 ccgctatcaa taattacatg gattatgaaa aggctcattc tgaagtatat aagtatgaag
    42901 aaaatgtcat tggccgcaga aatctatctc ctgtattgat tcgaaacatg attttagcta
    42961 tgattgcatt tgtttttgtt atcggcgtta ttctaacgat caatacgggt tggcttttac
    43021 ttttgatggg atttatctgt tgttttattg gagtattcta tacattcgga ccgattcctt
    43081 tatctcgaat gcctttaggg gaaattttca gtggggtgac tatgggcctt ggaatttttg
    43141 ccatggtcgt ttatgtcaat acattcggtt cagatgtcta tgatctgaca gtcaactttt
    43201 catctggaca atttgctttg gtaggaaatc tttgggctat agcagcaatc gtattagctt
    43261 ccttaccctt ggtgtttacg attgccaata tcatgttggc taataatttg cgggatcttg
    43321 accgtgatat tgaaaatcat cgctatacac tagtttttta tatcggacgt cctgcgggaa
    43381 cagtcttatt ccagctattg atgtatgctt gctatttcgt gattttaatg gggttgttga
    43441 gcggtatcta tgaatggccg atcttggtca ccttcttgac cttgccaatt gtgaagaagc
    43501 atttggatca attcaagcaa acgttacccc agcctatcag tttcagctat tccatcaaaa
    43561 atatggtctt attcaatgcc agttatgtat tggggctatt actaacaatc attatgcaag
    43621 ccatttaaca gcatgaagag gttgtgaaaa aactgccctg catcaagtaa taagaacagc
    43681 caaataaaaa tagctcctta tatttttcgt ttggttgggc ttattaccgc agatgcaagc
    43741 ttttgaacac cgtttatcag gaggttgtga aaaagtcgct tagtttcgag tattaagaaa
    43801 aattttgtga aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg
    43861 gcttttgaac accgtttatc agaggttgta aaatcagtgt tttgtcctga gtattagaga
    43921 aacaaatgga aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggacag
    43981 ctgattgaac actatttgtt cggaaaaagt gactaactat gacacaattt ttttcacagt
    44041 cccttttttc acttgatttg attcgtacca gcgaacttga cgagaacttt cttggtttgg
    44101 taactatatg gtattctttt aacatcctcg tttgcttctt ctaatttatt tatacaaagt
    44161 cacacttttt taaacatctg ttcttcgata catttctatt tcttttaaac atcgatacca
    44221 atattttgaa cttaagagtg cataccaaat ggattcattt actcttcgtc agttacgaag
    44281 ataatcaaaa tctccaactt ccctacttat ttcgttttga gaattatcga agcaatctta
    44341 cataaataac taaaaaacaa aaaaccagaa aatcagtata ctgattttct ggttttacat
    44401 ggtttgttca ataaaaagct gttggaactg ttaatattaa attatcgcat cgttacgaat
    44461 tcttccgctc ctgttggatg gatggctact gtattgtcaa agtcttcttt agttgcaccc
    44521 atcttgatag ccacggcaaa accttgcagc atctcatcga caccaacacc aatagcatgt
    44581 aagccgacga ttctttcttc ttttccgaca cagatcaatt tcatttcgca tttttggcga
    44641 taatcattta atgcaaagta cattggggta aagcgtgaac gataaacttt caattcattt
    44701 tcgccatatt tttccaatgc ttcttcttct gtcaaaccaa ttgttgcaat tggtggatgc
    44761 gtaaagacga ctgtcggaac aagtttatag tcaagataag aatctttctt tccattaaat
    44821 aaacgttctg ataaacgtct accagctgcg attgcaacag gagtcagatc taatttaccg
    44881 atcacatcac caacagcaaa aatatgttca tccgttgtat tttggtattt atctactttg
    44941 ataaaccctt ttttgtccaa ttccacattt gtattttcca gacctaattt ttcggtattc
    45001 ggtacacgtc caccggcaaa aagtacacaa tcaccagtta aggtttcacc attttcaaat
    45061 agcaccgtat attcgtcatt gtttttagta atttctttag caatatgatg tgcgtaagtt
    45121 tttattccag cttcttggta catttggatc aaattgtccg aaagcatttt atcaaacgtt
    45181 ctaagtggtc gttcttttcg atatgcccac atgacttcac tacctaaacc acaaaataca
    45241 cctgaaagtt cagccgcaat gtatccagca cctaaaacga ttgctctctt aggtaactct
    45301 gttaattcaa aaaatccatt tgaatcaatt gcgtattctc caccaggaat ggtcatttta
    45361 cttgcacggc ctccagtagc gatcaagatt ttaggagcac gataggtttc accgtttact
    45421 tcgatcgttt gactatcgat aaaagtagcg taacctttta tagcctcgac atgattgcta
    45481 tctaatcctc tttgataagc accgtgtaga aaatcaatat atttttctcg tctttctact
    45541 agaccggcga aatcaacatt ttttaaatct gcttggatac cgtaacttga ggcatctcgt
    45601 tcaattgttt ccaaaatcgt acttgcttgc cacataactt ttttaggtac acaaccgacg
    45661 ttgacacatg ttccgcccaa ttcattcgct tcaatcagca aaacttttgc gccatgcatc
    45721 cctgctctgt ttgctgaagc aataccgcca gagccgccac caattacgat gtaatcatat
    45781 atttgcattt tgttcctcct tattgaagag atcaatcaaa acctttattt aacgatcctt
    45841 tttctacaga aaacaagtgt aacacttttc ttgaattttg tatgcgaaga tgcttaaaac
    45901 agggatatat cagctttttc cttatttacg ttttcggatc atatctccag gtttcaccgg
    45961 ctgtgcgact ggcatagtta aaatcatcat tggatttggt gctcggtcaa tggattcgcc
    46021 ttcttcatta tgcataactt caacggtttg actgaaatga ctgaatcctg gaccataaaa
    46081 ttcgatttca tctcctacag aaaacaagtt tctctgacgt atcgtagcaa tctgtgttgc
    46141 ctcatcataa gataatactt caccgacaaa tttatatact gggattttac gacgttcacc
    46201 gaataattgt tcgttttcag ttggtgtatt gtagtaaaaa cctgtagcta attctcgttg
    46261 tgctactttc cataactcgt cgatccattc ttgcttgcag acatagtttt caggatcttc
    46321 catataactg tctacagccg ctttatagac atttgacacc gtagatacat aatggatcga
    46381 tttcattctt ccttcgatct tgaagctgtc tacgccgttt tcaatcaatt ctggaatatg
    46441 ttcgatcatt gacatatcca ctgcactcat cgagaattct tcttccactt cgcctgtatc
    46501 agttaaactg tttcgttctt gaccaaaagg catatcgaac aatccatact tccagcgaca
    46561 agattgagaa catccgccac ggtttgcatc ccgcatcgac atatggttgg acaatgtgca
    46621 tcgaccagaa taagagatac acatggcacc atggataaat gcttcgattt cggtatccgt
    46681 atttttacga atctctgcca cttcctccat tgaaacttca cgagctaaaa cgacacgttc
    46741 caacccttct tctttccaga attccaatgt ctcaaaatta gttgctgaag cttgagtgga
    46801 taaatggatc ggcagacctg gggcttctgt cgcacaaatt tcgatcaaag ctggatcaga
    46861 aacaatcact gcagaaatac cgacatcacg caaactgcgg aaaaattccc ctgctccatc
    46921 ttcatttcct tcatgggtga ccatattagc agcaacgtat accttcgcat catgttcttt
    46981 tgcaaaagca actccttcag ccatttcttc ataagtaaaa ttacctgcac ggcttcttaa
    47041 accatatgca ttcccgccaa tatatactgc atctgctcca taatggatcg cggtttttaa
    47101 cttttccagt gttcctgctg gcgctaggat ttctggtcgt ttcaattttc tttttactgt
    47161 catttctttc atcatactcc ttatttgata gcagaaggat caatataaaa gaatcctgta
    47221 tctaattcac ggttttcagg atgcaattta ctgatttcgc tagtcatttt ttccgctttt
    47281 tcttgatccc agctaccttc taaaatttgt ttttttgctt cggcgaatag tcgagtgatg
    47341 tttacgaatt tttcgcctgg agtaaataat ccgtccaatt tccatgtaca gtaatgatgc
    47401 tgataaagtt cattcaattc attcgagaga ttcagatcat tgctggcaaa aatatgtgtg
    47461 ccatgactat cttcataaat agaataatgt gtctcttctt ttttaggttc cgagataaaa
    47521 agacctcttt cacgatcttt ttgttcgtct tgtttcgtat agttatagta attctgtagc
    47581 agtggacgtt tggattgatg gatgcatgta gcaccataca caagggtttc tacaggaata
    47641 tcgagttttt cttccatcgc cttcatctct tcaaaaggaa cttcacgagc aagtactgct
    47701 ccaactgcgc cttttttcgc ccagaaattg atttgacgtg cgctcgttac taatgtttca
    47761 gcatcataaa tatacggtat acgcaattca ggattttttt tcatcgtata aataatacct
    47821 gtatcaccaa ctgtgatcac atctacgttt atttctttta aaaaagccaa atattcaggt
    47881 acatttttca ttttttcagg atgaatgatc ccatttacag caatattcgc ttttttacca
    47941 tacttgtgtg ctaattctac caattgtcgc tgttcttctc gtgtaaatga agcaggcaga
    48001 cgcaatccaa attgctcttc accgaaatat agtgtatctg cacctgcagc aagcaactgt
    48061 tctgcttgtt caatagtttc tactgtggtt atgagttcga tcataatttt ctcctcctac
    48121 aacttcataa tagtagcata gctctaaaaa ctttccaata cttcttctat ttaaagtcgg
    48181 atttttgaaa attatttcac aaaaaatcaa aataaagttc gatatattct gtccaaatat
    48241 aaaaagagat tgtgacaaaa atcttgtcac aacctcttaa tcaagtctta ctattataaa
    48301 aattcagcta cacaggccaa gtaactatag ggaacctact gtttcctttt taggtccgcg
    48361 aacattcaat gtgatcttac cttttgaagc acctactgtt acgtggtcac ctaaacgaat
    48421 ttctccgcca agcaaagcct cgctcaatcg atcttcaatt tctttttgaa gtgcgcgacg
    48481 gattggacgt gcaccatatt caggatcaaa tccagctttc ccgatcacat cgattgcaga
    48541 tggtgtgatt ttcacttgaa tatcttgttc tttcaatcgt ttgataatcg ctttgctcat
    48601 gatttggacg attgtatgga tttcatcttt actcaatgaa tggaagacga cagtctcatc
    48661 gattcgattc aagaattcag gtcggaaagc tttctttaac tcttctaaga tacgtttttg
    48721 catggctttg tgatctttag taacatcttg gacattgaat ccgacatttt tttcttctcg
    48781 aatttgtgtc gctccgatat ttgaagtcat aatcatgatt gtatttctaa agtccacttt
    48841 tctaccttta gaatctgtta aatgaccatc atccaatact tgcagaagta aattgaaaac
    48901 gtctggatgt gctttctcca cttcatctaa gagaatcact gaatatggtt tttgacgaat
    48961 tttttctgtc aactgtccac cttcatcata ccctacgtat cctggaggag acccgattaa
    49021 tcggcttgta ctgtattttt ccataaattc tgacatatca acacggatca gggcatcttc
    49081 acttccaaac atgacttctg aaagtgcttt ggccaattct gtttttccga caccagttgg
    49141 tccaaggaac atgaatgaac caattggacg atcaggatct ttcaatccgc ttcgtgcacg
    49201 gcggatcgca cgtgaaactg ctttgacagc ctcatcttga ccaacgacac gttcatgcag
    49261 aagtccttcc agttctaaca agcgttcgct ttcttttttc tccaactgct gtagagggac
    49321 accagtccac tccgatacaa ctgtagcaac atcttcttct gttacacgat cagaataacc
    49381 ttgctcctgt tttgcttcaa ttgcagaaac tttttgcagc tttttacgta gtgctttttc
    49441 ttgtctgcgt aattgagcag cattttcaaa gtcttgtttt tggatcgctg cttccttttc
    49501 ttgtactagt tcatcgattt ctagtttgat cacagttgat tttgttaagt gatcggtttg
    49561 atccaaacgg actttcgctg ctgattcatc gatcaaatca attgctttat cgggtagttg
    49621 acgagaagtg atataacgaa cagaaagatt gactgctgct cgtacagctt catctgtgat
    49681 ctccactcca tggtgctctt catatcttgt acgtaatcct tgaagaatca cttctgcttc
    49741 ttcaggagtt ggctcatcta cttgaatgcg agcaaaacgt cgctctaatg cagaatcttt
    49801 ttcgatatat ttttgatatt catctagggt cgttgctccg atcgtttgta attctcctcg
    49861 agctaacgcc ggtttcaaga tattcgaagc atcaattgct ccttctgctc cacctgcacc
    49921 aattaaggta tgcaattcat caatgaataa gattacttga ccatcattat agatttcgtc
    49981 gatgaccttt ttcattcgat cttcaaattc accgcgatat ttcgtaccag caaccaaaga
    50041 tcccatatca agcatcatca ggcgcttttc ttgcatgtct tctggcactt cacctaaaat
    50101 gattttttga gctaaccctt cagcaatggc tgttttgcct acaccaggtt cacccaccag
    50161 tacagggtta ttctttgtcc gtcggctaag aatctggata agccgtttca cttctctcga
    50221 acgaccaaca actgggtcca gcttgttttc ttttgctaat ttcgtcaaat cacgtgctaa
    50281 tgaatctagc gtaggtgttc cttgcggtgt ttgccgttta tcttgtacat ttacatttct
    50341 acgacgattc attccttttc cagcagaatc accagctagc cccatttttt gtttcagaac
    50401 ttgacgggct tttgaaaggc tgatacctaa gttgatcatg attcgagtag ccatgatact
    50461 ttcatctttt aataagctca tcaaaatatg ttctgttcca actttttgag aaccagtttt
    50521 cttcacttcg ctgctagcta aagcaaaaac ttgtttcatt cttggtgaat atggaagaaa
    50581 gactccttta gggtaagaac ggataccacc ataaccaatc agatgttcaa tttcttcata
    50641 aatttccgtt tcattcaaat ccatttcacg taatactttt ccagcaattc cattttgctc
    50701 gaccactaag gctagtaaaa catgttctga actaactgat tgatgtttca accgttttgc
    50761 ttcttcttgt gcaatttcta atactgcttg ggctctttca gtaaaaagtt cttccatcta
    50821 atctcacttc ccatcttcat aacttaaacg ttccagaaat gctcggagaa tccgagcacg
    50881 tgtatgatct tcatattcat tgaaattcaa tgtgtttttt gcaattgcac tcaacatcaa
    50941 gtttccttct ttttttgtaa ttatttggtc ttcataaagt ttttgaataa tagcaaaagc
    51001 atttttctca ctgattgtat catcaaataa ctgatttatc tgctcaagta attgtttttt
    51061 atctgaaatc cttacttttg caattcgtat ataaccacca ccaccacgtt tgctttctac
    51121 tagataacca cgttggatgg taaaacgggt attaatcaca tagttgattt gtgaaggaac
    51181 acagttaaag aggtctgcca tttcaatcct tcgaatctcg atcatttcac tattttccag
    51241 aattttcttt atatatgatt caattaaatc cgaggtattc tgatgtgcca tcttattcat
    51301 tccctttgac taatattgac cttaattata acgaatttga taatagaaag aaagaagaac
    51361 gctttttgtt aaataaatcc tttagaatca acaaaaaaaa atacggaata tactattccg
    51421 tatc
//
LOCUS       DAHUVV010000010        49902 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.010, whole genome shotgun sequence.
ACCESSION   DAHUVV010000010 DAHUVV010000000
VERSION     DAHUVV010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.010"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..97)
                     /locus_tag="NK767_000565"
     CDS             complement(<1..97)
                     /locus_tag="NK767_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCH6693580.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQ"
     gene            complement(99..296)
                     /locus_tag="NK767_000566"
     CDS             complement(99..296)
                     /locus_tag="NK767_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HCH6693581.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(690..2417)
                     /locus_tag="NK767_000567"
     CDS             complement(690..2417)
                     /locus_tag="NK767_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HCH6693582.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2410..3603)
                     /locus_tag="NK767_000568"
     CDS             complement(2410..3603)
                     /locus_tag="NK767_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HCH6693583.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3790..4434)
                     /locus_tag="NK767_000569"
     CDS             complement(3790..4434)
                     /locus_tag="NK767_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCH6693584.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4528..4662)
                     /locus_tag="NK767_000570"
     CDS             complement(4528..4662)
                     /locus_tag="NK767_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCH6693585.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4664..6796)
                     /gene="feoB"
                     /locus_tag="NK767_000571"
     CDS             complement(4664..6796)
                     /gene="feoB"
                     /locus_tag="NK767_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCH6693586.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6793..7266)
                     /locus_tag="NK767_000572"
     CDS             complement(6793..7266)
                     /locus_tag="NK767_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCH6693587.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7667..7891
                     /gene="nrdH"
                     /locus_tag="NK767_000573"
     CDS             7667..7891
                     /gene="nrdH"
                     /locus_tag="NK767_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCH6693588.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8050..10209
                     /gene="nrdE"
                     /locus_tag="NK767_000574"
     CDS             8050..10209
                     /gene="nrdE"
                     /locus_tag="NK767_000574"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCH6693589.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10259..11224
                     /gene="nrdF"
                     /locus_tag="NK767_000575"
     CDS             10259..11224
                     /gene="nrdF"
                     /locus_tag="NK767_000575"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCH6693590.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11313..12452)
                     /locus_tag="NK767_000576"
     CDS             complement(11313..12452)
                     /locus_tag="NK767_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCH6693591.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12761..13474)
                     /locus_tag="NK767_000577"
     CDS             complement(12761..13474)
                     /locus_tag="NK767_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCH6693592.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13728..14429)
                     /gene="nagB"
                     /locus_tag="NK767_000578"
     CDS             complement(13728..14429)
                     /gene="nagB"
                     /locus_tag="NK767_000578"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCH6693593.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14663..16195
                     /locus_tag="NK767_000579"
     CDS             14663..16195
                     /locus_tag="NK767_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCH6693594.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16386..17588)
                     /locus_tag="NK767_000580"
     CDS             complement(16386..17588)
                     /locus_tag="NK767_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6693595.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17670..18359)
                     /locus_tag="NK767_000581"
     CDS             complement(17670..18359)
                     /locus_tag="NK767_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCH6693596.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18465..18833)
                     /gene="rplL"
                     /locus_tag="NK767_000582"
     CDS             complement(18465..18833)
                     /gene="rplL"
                     /locus_tag="NK767_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCH6693597.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18886..19389)
                     /locus_tag="NK767_000583"
     CDS             complement(18886..19389)
                     /locus_tag="NK767_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCH6693598.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19593..19720)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19799..20488)
                     /gene="rplA"
                     /locus_tag="NK767_000584"
     CDS             complement(19799..20488)
                     /gene="rplA"
                     /locus_tag="NK767_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCH6693599.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20592..21014)
                     /gene="rplK"
                     /locus_tag="NK767_000585"
     CDS             complement(20592..21014)
                     /gene="rplK"
                     /locus_tag="NK767_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCH6693600.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21357..22025
                     /gene="sdaAB"
                     /locus_tag="NK767_000586"
     CDS             21357..22025
                     /gene="sdaAB"
                     /locus_tag="NK767_000586"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCH6693601.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22038..22910
                     /gene="sdaAA"
                     /locus_tag="NK767_000587"
     CDS             22038..22910
                     /gene="sdaAA"
                     /locus_tag="NK767_000587"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCH6693602.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23001..24020)
                     /locus_tag="NK767_000588"
     CDS             complement(23001..24020)
                     /locus_tag="NK767_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCH6693603.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(24193..25098)
                     /locus_tag="NK767_000589"
     CDS             complement(24193..25098)
                     /locus_tag="NK767_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCH6693604.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(25157..25702)
                     /gene="nusG"
                     /locus_tag="NK767_000590"
     CDS             complement(25157..25702)
                     /gene="nusG"
                     /locus_tag="NK767_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCH6693605.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25803..25973)
                     /gene="secE"
                     /locus_tag="NK767_000591"
     CDS             complement(25803..25973)
                     /gene="secE"
                     /locus_tag="NK767_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCH6693606.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25997..26149)
                     /gene="rpmG"
                     /locus_tag="NK767_000592"
     CDS             complement(25997..26149)
                     /gene="rpmG"
                     /locus_tag="NK767_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCH6693607.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26311..26955
                     /locus_tag="NK767_000593"
     CDS             26311..26955
                     /locus_tag="NK767_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCH6693608.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27011..27514)
                     /locus_tag="NK767_000594"
     CDS             complement(27011..27514)
                     /locus_tag="NK767_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCH6693609.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27527..27573)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28111..29076
                     /locus_tag="NK767_000595"
     CDS             28111..29076
                     /locus_tag="NK767_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCH6693610.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29175..30347)
                     /locus_tag="NK767_000596"
     CDS             complement(29175..30347)
                     /locus_tag="NK767_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6693611.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30334..31479)
                     /locus_tag="NK767_000597"
     CDS             complement(30334..31479)
                     /locus_tag="NK767_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6693612.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31493..32417)
                     /locus_tag="NK767_000598"
                     /pseudo
     CDS             complement(31493..32417)
                     /locus_tag="NK767_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32534..33613
                     /locus_tag="NK767_000599"
     CDS             32534..33613
                     /locus_tag="NK767_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCH6693613.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33645..34238
                     /locus_tag="NK767_000600"
     CDS             33645..34238
                     /locus_tag="NK767_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6693614.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(34348..34575)
                     /locus_tag="NK767_000601"
     CDS             complement(34348..34575)
                     /locus_tag="NK767_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCH6693615.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34678..37266)
                     /locus_tag="NK767_000602"
     CDS             complement(34678..37266)
                     /locus_tag="NK767_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCH6693616.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37286..37945)
                     /locus_tag="NK767_000603"
     CDS             complement(37286..37945)
                     /locus_tag="NK767_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCH6693617.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(37994..38497)
                     /gene="trmL"
                     /locus_tag="NK767_000604"
     CDS             complement(37994..38497)
                     /gene="trmL"
                     /locus_tag="NK767_000604"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCH6693618.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38718..39584)
                     /locus_tag="NK767_000605"
     CDS             complement(38718..39584)
                     /locus_tag="NK767_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCH6693619.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39693..40334
                     /locus_tag="NK767_000606"
     CDS             39693..40334
                     /locus_tag="NK767_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCH6693620.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40396..41757)
                     /gene="mgtE"
                     /locus_tag="NK767_000607"
     CDS             complement(40396..41757)
                     /gene="mgtE"
                     /locus_tag="NK767_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCH6693621.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41775..42674)
                     /locus_tag="NK767_000608"
     CDS             complement(41775..42674)
                     /locus_tag="NK767_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCH6693622.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42676..43473)
                     /locus_tag="NK767_000609"
     CDS             complement(42676..43473)
                     /locus_tag="NK767_000609"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCH6693623.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43451..44155)
                     /locus_tag="NK767_000610"
     CDS             complement(43451..44155)
                     /locus_tag="NK767_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCH6693624.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44286..44861
                     /locus_tag="NK767_000611"
     CDS             44286..44861
                     /locus_tag="NK767_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCH6693625.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            44979..45656
                     /locus_tag="NK767_000612"
     CDS             44979..45656
                     /locus_tag="NK767_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCH6693626.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45735..47543)
                     /gene="pepF"
                     /locus_tag="NK767_000613"
     CDS             complement(45735..47543)
                     /gene="pepF"
                     /locus_tag="NK767_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCH6693627.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47719..48717)
                     /locus_tag="NK767_000614"
     CDS             complement(47719..48717)
                     /locus_tag="NK767_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCH6693628.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(48943..49596)
                     /locus_tag="NK767_000615"
     CDS             complement(48943..49596)
                     /locus_tag="NK767_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCH6693629.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14936 a        10474 c         8765 g        15727 t
ORIGIN      
        1 tttgtaaaac ctcaagaata tcagaatatt taggatcttt acttatagat atatcattat
       61 ataattcatg aacaatttgc ttagcatttt ttttcatttt agcacttccc tggaattgct
      121 ccacctaaaa aaccacctat agacattcct gcaatacaag tagttgcctt ggcccaatcg
      181 accgtacatt tatttttagt gcaatacact ccatttccat aatattttcc actatgagtg
      241 gtacccccat agataagttg tgtctcttta atagacaaaa tttttaaatg tttcataatc
      301 taatcacccc taaattaatg tttttagact attattctaa cattcattaa atttcaaaaa
      361 acaattcctt cttgaaacgt agcaaaataa ttcctgaata agaatttaat cacttttaag
      421 tatataaact atgtatattc tttaatattt tttgagaatg cacttcgtac ataataaact
      481 ttaatcacat tagtctttca tacctacgat cgatctcttt gactcgcacc cataaattca
      541 taatagtttg tcggcgtctt tggacgtttc gaagatgagc tacgcattct ctttttttat
      601 agacaaaaaa gatagagacg aacatggagc ttccaaattt ggatatttga tccaattttg
      661 gaagctccat ttatttcgtg tctgtctttt tattttttcc agctttcgac taattcttgg
      721 ttctctttga tccagtcttt tgctgcttgc tgtggatctt ttccattgtt gatttccaac
      781 ataacttttt ccatatcttt ttctgaccaa tggaaattgt ctaatacttt gtaagcttct
      841 ggctggtctt ctttcaatcc ttttcgaacc atcgtatgga tctgctcttc gcttcccatt
      901 ccgttttcag gatcttctaa gtatttcaga tcatatttgg caaacatcca atgaggtgtc
      961 catcctgtaa ctacaattgg ttcgtgtttt ttgatcgctt gacctagtgc aacggtcatt
     1021 gctcctgaag aagatgtctc tactttccag tctttcaagt tgtcatattt ttggatcgtg
     1081 ttttcagcag ccgtaactac accagctcct ggctcgattc ctatgatttt cttacctgcc
     1141 tgatcggtta actcatcgat cgaatttaca tccatgtagg ctggcactgc taaaccaact
     1201 tttgctcctg tcaaattggc acctaggtct tcgacttgat ctttatattg tgcatattgt
     1261 gaaccatgag ttttaggtag ccaagctgaa accatggcat ctgactcgcc atttgaaaca
     1321 gatttccaca tgattgaatt atctaatggc gtcatcgtta catcatagcc catttgtttc
     1381 aatacttcac cgactacatt tgttgaagca acttctgtat cccattcaac ataagataag
     1441 ttgatacgtt ttgtttcttt ggcagatgaa aaactcccta taccaatcgc acctaagatc
     1501 actactacta cagcaccaat gatcagtcct tgacgttttt tacttttctg tgttgttgct
     1561 cctgcagctt ttttattgtt tggctggttc aaatgctgtg taaaacggtc aatgatgatt
     1621 gctaagataa ctaatgcaac cccgttgaca aatccattcc cgacttgtgc tctttgcaat
     1681 gctgataata cgccgcgtcc taagccgggt gcaccgatca tagaggcaat aacgaccatt
     1741 gaaagtgaca gcattgttgt ttgattgatc cctgccatga tcgtactttt agctaaaggc
     1801 aattctagtt tgaatagttt ttgacgactt gtgctcccga atgaatcaga agcttctact
     1861 aattctttag gaacttgtcg aatccctaaa ttcgtaaaac gaacggttgg tggcagagca
     1921 aagatgactg aggcaaatac ccctggaacc atcccgatac cgaaaaatgc aactgctgga
     1981 atcaaataaa cgaatcctgg cattgtctgc ataaagtcaa ggatcggttt gataatactt
     2041 tgggctttac tgctttttgc catcaagatc cctaatggta ccccaatgat gatcgagatc
     2101 acactagata aaagaacaag tgtgacagta ttcatcaaat cattccatag accttggtta
     2161 taaataaacc atagaccgat taatgtgaac aacgtaagtc ctggacgttt cttagaaata
     2221 aagaaagctg ctaccgtcaa taacaagata aacaaaggag ctggaatcac taacagtaaa
     2281 tttgtaatcc ccgacatcac tgcctgtcca atcgtttgta aaaatgaaaa taggccagaa
     2341 aaagttgtcg taaaccattc tgtgatattt tctacccagt ttgccactgg taattgatag
     2401 ttcaatagat tattcatgtt cactcacctc gcttgtttct gctaatgctt caatcacact
     2461 acctcggatg acgacacctt ttaattttcc atcctctaca actgcaagtg gagtcggtga
     2521 gtcataaata atattgaaaa tatcacttac caccatgtct ttcccaatca ccgtcacatt
     2581 ttcatctagg aagtcgatca atggacgttt ttgcttccta gcttctaacg cttgatctgc
     2641 agtgatgatc cctttcaggt gtctctttct gtcaacagcc atcagcatgc tgacttcttc
     2701 attacgcatc cttgtcaatg caacgtttgg tccatcagat tcgatattcg tcgtcaatgc
     2761 aggtaccatg atgttttgag ccgtaagcac tttggaacga tcgacttctt ccacaaattc
     2821 acgtacataa tcatttgctg gattggtcaa tatttcttca cctgttccga tctgcatgat
     2881 ttcgccgtct ttcatcaagg cgattctgtc cccaatccgt aaagcttcat tcaaatcatg
     2941 ggtaatgaaa ataatcgttt tttgaacatt ggcttgcagg tccagtaatt cgtcttgcat
     3001 ttctcgccgg atcagcggat caagagccga gaacgcttcg tccatcagca agatctcagg
     3061 atcatttgct aaagctctgg ctaggccaac ccgttgttgc atcccaccag ataactgact
     3121 aggatattgg tctttaaatg aaagcaaact agaattttct aatgcttttt ctgctttttc
     3181 ttgtcgttct tctttaggta caccacggac ttctaacccg tattctgtat tttccaagat
     3241 cgttcgatgt ggaaacagac cgaagttttg gaaaaccata ttgattttat gacgacggac
     3301 ttctcgcaat tcttctttgc ttaacttcga tacatcttct ccatcaatat agatactgcc
     3361 cgaagttggc tcaatcagac gattcaatag acggatcaat gtggatttcc cactcccgga
     3421 aagccccatg atcacgaaga tttctccttc ttttacgtca aaattcacgt catacacacc
     3481 aaccgttgca ccggttttct ttaaaatctc cgttttcggc tggtgatcat tcatcatatc
     3541 taaagcggct tgtgtctttt taccaaatat ctttgttaaa tgttttactt ctacttttac
     3601 caaacaaatt ctcctttttt atgtaaattc atttttcttt ctgaaaaaag tgaccacact
     3661 atgttaacat catgtagtca ctttgtcaaa aatgaaagaa atttgcttgt ccaaactgaa
     3721 aaaaacttgg ctaaatcttt gtaaataaaa ggtttagcca agttaatatt tttttaatat
     3781 ttcacacaat cattctgcaa gctgatgctc cgggtaaaaa aagtttgtca tacgctgtag
     3841 tgtatattca tttccaataa aatgaacgat gtcccctgcc gtaaatttag cataggggcc
     3901 aggtgaaaca agcagttctt cattatgaga aattgcgata actgtggcgc cagtcgcctg
     3961 ccagatattc aactcgctaa ttgaacggtc cagatgcatt gctttttctg ataaagataa
     4021 ttcaaacgga agtaaaggat tcatcttatg tattcgcttc gtttgattga ccagctgatc
     4081 caataattga gaaaaatgat tcagctcatc ttgttgcttc tccacacttg ccatgatttc
     4141 tgcttttgta tcttggatgg attgtacatc ttgatattgc aataggaaat cttttgcttt
     4201 ttccttcgaa gcaacaaatg ccccacttcc atgccgtact tccatgattc caagatctac
     4261 cagcacattg atagccttgc gggtagtctc aggagaagca ttgtacgtac tggcaagtgt
     4321 tgagcgggca tggatcttct cacccacttt atatcggttt tcaacgatac gttcggctaa
     4381 atcaacagca atctgctggt agcgcgggcg gacgattttt ttctttccag acattttctt
     4441 tcctctttct ttttttactt tccctcatct tactatgctc tagcaaaata agcaaaaaag
     4501 gggagaatag cctcttgcaa cgactgatca tcgcttgatc tgttcctttt ttactgggca
     4561 agctgtttga cagtcatcac aattcttacc ggattttaaa cgagtataga caattgcgcc
     4621 tgcacttcca aagattaaca cacttaataa gaatgtcgcc atcctattcc tctccttcta
     4681 atgacaatat agtataaaac tggttcttgg attttggctg acggaacaaa acataaacga
     4741 gtcctatcat cagtaaaata gcaagatacg tccctaacgc tacctgtcca ccctccaata
     4801 agtgtcccaa ctgataaatc acaaaactaa ctaaataagc taaaccgcat tgatagccta
     4861 tggcgatcca agtccatttc ttggcattca tttcccgatg gatagcccca attgccgcaa
     4921 aacaaggcgc acaaagtaaa ttgaaggcta acaatgaata agctgctgcg ggagtatatg
     4981 tgtgttgtag cacttgccag atttctctgc cattttcgga tacttccgca tgaccaaaca
     5041 aaataccaaa tgtgccaata atattttctt tagcgaccaa tcctgtaatc gctgcgactg
     5101 ttccttgcca gtttccccaa ccaagcggct gaaaaacaac tgccaggatt cgcccgaaaa
     5161 aggctaagat actttggtct tcagaaacag gttgagcata aaaattataa gaagagctga
     5221 accagatcac gatactagaa acaaagataa tcgttcctgc ttttttaaca aaagacttgc
     5281 tgcggtcata ggtttgtctt aggaccgctg accattgagg aaaatgatat gcgggcaatt
     5341 ccatgataaa aggagctgta tctccagcaa acaaacgtgt ttttttcaag ctgattccag
     5401 agaaaataat tgcagtcata ccaagaaaat aggcagaagg agctacccaa gattgatgag
     5461 gaaaaaaggc ccctgctact aaggcaataa tcggcaactt tgcagaacaa ggcatgaagg
     5521 ttgtgaccat gatcgtcaaa cgacgatctt gctcgttttc aatcgtgcga ctggccatga
     5581 cgcctggcac tccacatcct gtagcaatca gcataggaat gaaggatttt cctgaaagac
     5641 cgaatttcct gaaaagtcga tccatgacaa aagcgatcct cgccatatat ccgcagtctt
     5701 ccagaaagct caagcataaa aacaatataa tcaattgcgg tagaaatcct aacactgctc
     5761 ccacgcctgc gatgatgccg ttcaagatca gctcctgcat ccatcccgct acttgccatt
     5821 cagctaacaa tctgccaaca tgttctggta accatgtacc aaacaatgtg tcattgatcc
     5881 aatctgtccc cattgttcct actgtttgaa tagccagata atagatcagc cacatcacaa
     5941 atgcgaagat cggcaaggct aaccaacgat tcgttgcaat ctgatcgatt ttgtcactca
     6001 tcgataattg gaaagaatca tttgacacaa cacagagtgt acatagttgg gtaataaatt
     6061 cataacgttc attgaccaga atcgtctcac tatcgtcgtc caatagtttc tctgttaact
     6121 ggatcagttg ctcgatctct ttttgctgag aaactgctat ctcgtaatcc tctcttacct
     6181 tctgatctcg ttcaaaatat ttcattgcat accagcgttt cttgccgctt tgttctccta
     6241 gtacatcttc aatttcatgt aaagctgctt ctaaacgtgg atcatatgta cagggaacag
     6301 gaatagaagg ccgctttttt gcagactcct gaatcttttc gattagtttt tcgattcctt
     6361 gctttttcaa tacgctgatt ggtaaaactg gtacaccaag accataggaa agcttttcta
     6421 gattgatttt ttttccttct tttttgatga catccatcat attcagacca atgatcatag
     6481 gaatgtctaa atccaataac tgcgtagtta aataaagatt tcgttctaaa ttggtagcat
     6541 caataatgtt gatgatcagt tcaggctgtt cctgaaccaa ataatctctt gcaatcactt
     6601 cttctggtgt ataaggagac aaagaataga tgccgggtag gtcctgaata tgaatgttcg
     6661 gatttttttt caacgatcct tctttttttt ctactgttac gcccggccaa ttccctacat
     6721 attgattcga accagtcaag agattgaaca agctagtttt cccgctgttt ggatttccga
     6781 ctaacgcgat attcattctt tttcctccct ttgtatcaag attagttcgg cctctgactt
     6841 tcgtaacgtc agttcatacc ctcgcactgt cagttcgatt ggatctccca gaggggctag
     6901 ttttcggata actattttta catttcgcgt caatcccata tccattaacc gtcgcttcac
     6961 tgcgccttgt ccgtggattg ctacgacgat cccagtctct cctgctttta attgatcaag
     7021 agaagtccag ctttcagagg aagcagtctt tttattgaca aagatttgac ctagtatttc
     7081 ccgacttact gccactcgat gactttttac cagtaaaatg gcattgttgc ctgataaatt
     7141 gacgacgacg attttacttc cggcaacgat cccgatattt tgcaaatggc gttttgtttt
     7201 ttcttctgtt tcgattcgtt cgacttgata aacgaaattc tgctttgcct gcgctaaggt
     7261 ctgcatattg attcctctct tgtgaaataa atcatgtatt aaaatcattc attatatgtt
     7321 ctaattttct tacgtgttac gcaaataaat gagaatcatt ctcacataga atactctcgg
     7381 taattgagaa tgtcaattat acttgtgata ttatgagcga atctttagta ttgatagata
     7441 aaaatgagaa aaaaatattt cttcatagtt ttccacaatt ttttctctat taaatttgct
     7501 attttcccac acgatacttg ttttcagctc ttttttcaaa aacacaaaac ttagtattcc
     7561 acagtttcca caacacaata tgtagtgttt gttgtttgac ttttttacta agagttttat
     7621 actatctatt atagataata agaatgtgtt agaggaggca ctcaccatga acgtaaaaat
     7681 cttttctaaa aataattgta tgcaatgtaa gatggtcaaa cggtttttaa cagaaaatca
     7741 aattgctttt gaagaagtca atatcgatga acagcctgaa gcagtagact ggttgaaagc
     7801 acaaggattc caaagtgttc ctgtcattac ttctgatgca gaaacagtta tcggattccg
     7861 tcctgatcag ttgcgtaaac ttgccagcta acatgactaa aagaatcaaa acgtgataca
     7921 tggatttgaa gggcttgaga cagcaccagt ttttgtcgca aacttccaaa tcctttttta
     7981 aagcttaaaa acaatgcaat gatttgcaat caagcatcat caaatacgaa atgaaggttg
     8041 ggcgtacgta tgagtcttaa aaatttacaa gatgtcagtt atttcaagct gaataatgaa
     8101 atcaatcggc cagtcaatgg tcaaatccct ttgaataagg ataaagaagc tttagttgcc
     8161 ttttttgagg aaaatgtcaa accaaatacg atgacttttc tatctattat ggaaaaaatc
     8221 aattacttga tcgatcaaga atatatagaa gcagaattca ttcaattgta ttcaccatca
     8281 tttattgaaa agctatatca atttttggac gagcaacatt tttctttcaa atcttttatg
     8341 gctgcttaca aattttattc tcaatatgcg ttgaaaacca acgacggttc cgcttatctg
     8401 gaaacttatg aagatcgagt agccttcaat gcgctttact ttgcgaacgg ggatgaagaa
     8461 ttagcacttg ctcttgcaga cgaaatgatc catcaacgat atcagccagc tacaccttct
     8521 ttcttgaatg ctggacgtaa acgtcggggc gaattggtgt cttgtttctt gactcaagtc
     8581 actgacgata tgaactctat cgggcgctca atcaactctg ctttgcaatt gtcccgtatt
     8641 ggtggcggcg tagggattac tttatcaaat cttcgtgaag ccggcgctcc tatcaaagga
     8701 tatgagggtg ctgcaagtgg tgtcgttcca gtcatgaaat tattcgaaga tagtttcagc
     8761 tattctaatc aattaggtca gcgtcaagga gctggcgtgg tttatttgaa cgttttccat
     8821 ccagacatta ttgctttcct ttcagcaaaa aaagaaaatg cagatgaaaa gattcgcgta
     8881 aaaactcttt ctcttggcgt gatcgtacct gataaatttt atgaattgac acgtaaaaac
     8941 gaagatatgt atttatttag tccatatagt gtcgaaaaag aatatggtgt cccattctct
     9001 tatgtaaata ttacagctga gtatgatcat ctagttgcaa atccaaacat tcgcaaacaa
     9061 aaaatcaaag cgcgtgatct tgaaaatgaa atctcaaaat tacaacaaga atctggctat
     9121 ccttacgtaa tcaatattga tacagcgaac cgcgagaatc caatcgatgg caagatcatc
     9181 atgagtaatt tatgttcaga gattttacaa gttcaaacgc cttctgtgat taacggcaaa
     9241 caggaatatg aagtattagg aacggatatc tcatgtaact taggctcgac aaatatcgtg
     9301 aatttgatgc aaagccctaa ctttggtcaa tctgtgcgta cgatgactcg tgcattgaca
     9361 tttattacag atgcatctga tatcgatgtc gtaccaccga tccaaaacgg aaataagctg
     9421 aatcatacga ttggattagg tgccatgggc ttacacactt atcttgcaaa agaacaaatg
     9481 gaatatggct cagaggattc attagacttt acaaatattt acttcatgct gttgaattat
     9541 tggacacttg ttgaaagcaa caatatcgct cgtgaaagaa aacaagtttt ccataatttt
     9601 gaaaaatcag cttatgctga cggcacttat ttcgaaaaat acgtaactgg agaattccag
     9661 ccaaaatcag aaaaagtcaa agcattgttt gaaggcattt ttatcccaac tgctgaagat
     9721 tggaatgctt tgaaacaagc agtaatgaaa gatggactgt atcatcaaaa tcgcttggca
     9781 gtagcaccaa atggctcaat ttcatatatc aacgatacga gtgcaagtat tcatcctatc
     9841 actcgcatga tcgaagaacg ccaagagaag aaaatcggta aaatttatta cccagcgcct
     9901 tatctatcca atgagacgat tccgtattac acttctgctt atgatatgga catgcgtaaa
     9961 gtaatcgatg tttatgcaat ggcacaacaa cacgtcgatc aaggcatgag tttgacttta
    10021 ttcatgcgtt ctgaaatccc agaaggcatt tatgaatgga agacaacaac gaagcaaacg
    10081 acacgtgatt tgaatatttt aagacattac gctttccaca aaggaatcaa atccatttat
    10141 tacgtacgta cctttactga tgatgcagaa gaaatcggaa gcaatcaatg tgaaagctgc
    10201 gtgatctaat atcatagata gattcgctta ttcatatttt aattaaagga gcattctcat
    10261 gtcagaaaca tattatgaag caataaactg gaatgaaatt gaagatatca ttgataaatc
    10321 cacttgggag aagctgacag agcaattttg gctagataca cgtatcccac tttcaaatga
    10381 cctagatgat tggcgtacat tgtctcaatt ggaaaaagat acagtaggac atgtatttgg
    10441 cggtttgaca ctgctagata ctgtccaatc tgaaagtggc atggatcagt tgcgtaaaga
    10501 tgtccgcact ccccatgaag aagccgtttt gaacaatatt caattcatgg aatccgttca
    10561 tgccaaaagt tattcttcta tttttagtac actgaatacg aaaaaagaaa tcgaagaaat
    10621 ttttgactgg acaaatacga atccttatct gcaaaaaaaa gctgagcgta tcaatgaaat
    10681 ctacaaacac ggtacaccgc tagaaaagaa aattgccagc gtctttttag aaacattctt
    10741 gttctattca ggtttttata ctcctcttta ttatttagga aacaacaaat tggctaacgt
    10801 agcagagatc atcaaactaa tcatccgaga tgaatctgta catggaacct acatcggtta
    10861 caaattccaa ttaggattca acgagttacc tgaagcagaa caagaaacgt taaaagactg
    10921 gatgtacaat cttctttatg agctttacga aaacgaagaa cgttatacag aagaattata
    10981 cgatccaatc ggctggacag aagaagtcaa gacattccta cgctataatg ccaacaaagc
    11041 attgatgaac ctagggatgg atccactctt tccagatact gccaatgatg tcaacccaat
    11101 cgtaatgaac gggatttcaa caggcacaag caaccatgac ttcttctcac aagtcggaaa
    11161 cggctatttg ttaggtcatg tagaagcgat gaaagacgac gattatttga ttggattgga
    11221 ataaggattt attttaattc aaaaattttt cgaaaaataa aaagagttta gcgtacagat
    11281 attttaaatg tacactgaac tcttttttat gcttattcac ttgtttcttc cagcactttt
    11341 ggactgtcta acttttccga aacatgttcc attcttctca ataaatagat tttccggata
    11401 tttaatatta tcgtcttaat cactgcatag acaggtatgc aaatcagcat gcctaatatt
    11461 cctgcgacac ttcctgctcc aatcaatacc aagatgatcg tcaaaggatg gatattcatt
    11521 gatttcccaa ataacaacgg cttgatcaaa tttccatcta attgttggat cactaaaatc
    11581 gacaacgcca tatacaaagc ttgaaatgga gagataaaca aaccgacaat gattgctggt
    11641 accgctccaa taaaagggcc gacatacggg ataatattcg tgattccgca aatgaccgca
    11701 agcaacaagc tataaggctg atcgaatatt ctcattgcca aaaagctcat tacaccaata
    11761 atcaaagcat ctaagatcgt actgctgata taggcagata atgtttcgtt taattctttt
    11821 accgtttggc gtaactcttt acgaatcgct ttcggaaaga agtgagacaa cgcatcaata
    11881 aatttgtgcc catccttgaa cataaatagc aaaataaatg gaacggtaaa taacaaaata
    11941 aaaaattgca tcataaatga aatgattttt gagacacttg cagataaact gactaatatg
    12001 atattgaaca aattactaag agataaattt gctgatgcta attgttcttc caaattgaaa
    12061 tttttaaact cttcccgctg cgccaattca tttaaccatt tgcttgtttc atctgcatac
    12121 attggcaagc tctgggtcaa ttgaattgtc tgttccatta actgaggcac tacattcatg
    12181 acagttaaaa caactaaaat gattacgacc aaaaacgtca ataaaaaacc gaacactctc
    12241 gggatccgat gcttttcgag aaaaacaacg attggatcga acatataata gataaaacca
    12301 gcaacaagca atggtaaaaa aactgccgaa agcattttca atattggact aaaaatattc
    12361 ggcatttgta aaataaagta cacgccgatg atcgtgagta ctagctccac tgtccagaaa
    12421 aataattttg attgtttaaa acgtgattgc atacatctcc tccaaggtcc tttgatactt
    12481 ctattctatc atttaacaaa gagttacgcg ctgagatttt catttgctta aaaaaaacaa
    12541 aaaaaataaa catattttaa tgagtagacg agtacttaaa caaaaacacg gtttggagta
    12601 gtgacaaaaa ttatactgca gtcccttttt ccaaataaac ggtgttcaaa agcttgcatc
    12661 ttcgtaataa gcccagccaa acgaaaaata taaggaacta tttttatttg gctgggctta
    12721 ttacttgatg taggacatct attttacaac ctcagatcat ttattgcgca atactgacta
    12781 aatgcaactt tgtgatattt ttgtgtacgg acaactcctc gatcacatcg gcataagtga
    12841 gcccttttgg aagatcaatc gtatagatat ttttgtacac tcggtagtca tcaacgaatt
    12901 ttacgtcaaa attcacatct tcgattacga tatttttctc ttcaaaataa tcggtaagaa
    12961 aatctttggt ttcttttcta tggataaatt ggatttctaa ctttttagtc gtaggtacat
    13021 ggatcacgcg tttcactact gtgagtgcaa tagcaatccc gataaaactg atgatcgcaa
    13081 tggaatagta acccatgccg atggcgatcc ctaatccagc aattgcccat aaagaagcgg
    13141 cagtcgtaag tcccgtcacc gattgtttcg tgacgatgat cgttcccgcg cctaagaaac
    13201 cgacaccact caccacttga gcaatcagcc ttgcttcatc tgaacgaatc gttccagcaa
    13261 gctgtggata ctctaaagca tctcttaatg caccactagc gatctcaact tggatcaatg
    13321 caatgataca tgctcccata caaactaaaa tatgcgtacg catacctgct ggacgatttt
    13381 tatattgtcg ttcaaaaccg attgctccgc caaatagcat agcgatccct aaacgtaaaa
    13441 taatctctgg aattgtcaat accatatcat tcatctaaac accctccagt ccaatatatc
    13501 atgtctgaaa ggagcagaca aaaaaagtgc aatttttacc tcttacgaaa aaaggactgt
    13561 gacaaaattt ttgtcatagt cctttttatg aataaacggt gttcaatcag ctgaccctcg
    13621 gtattactca acaaacggca aaatatgggg agctattttt cctttgtctt ctctaatact
    13681 caggtcaaaa cgctgatttc acaacctctt tagcctacta ttgaaaatta caacttactt
    13741 gctgcttctt catcgatgat cacaacaaca ttgtcgtgat tttgcaatac acttgctggc
    13801 aaatgattcg ttacaggtcc ttcaatcatc cctttgattg catcagcttt gctttcgcca
    13861 aatgccatca agacgatttc tttacttttc ataattgaac cgattcccat tgaaacagct
    13921 tttgttggta catcttctgc tttatcgaag aatcgtttgt ttgcattgat tgttgattct
    13981 gttaagtcaa ctactgaagt cgttccgtca aatgaagcac caggttcatt gaaaccgata
    14041 tgtccatttt gcccgattcc taagatttgg atatcgattg gatgtgcatc gatgatttga
    14101 tcatactttt tgcatgctgc ttccagatct tctgcttttc catctggtac aaacgtttct
    14161 ttgaatggtt ttttatcaaa taattggaca tccatgaaat gacggtagct ttgttcatct
    14221 gaaccgccta gtccaacgta ctcatctaag tttacagaag tcatttcact gaaatccaaa
    14281 tcgcttgaag tcatttcttt atataaagta actggtgtgc ttcctgttgc caatcctaat
    14341 acttttgcac ctttgttcat accttctttg atgatttcaa atgctttttt tccgccttct
    14401 tcagcgtttt ttacacgaat gatttccata gtgattgctc ctttatattt tggtatagcc
    14461 caattatagc attctttttt tctttttaca ataattagta tagaccaaat tgcatttttt
    14521 tcattttctt gacaataaat aggaatccgt gttaggtttc ataagatggt tcaatcaaac
    14581 aatccatgtt gcatgaaaac caagcaagtg attcactgtg gacgctttat gtctgcagtt
    14641 tttttatgaa aaaggagtta ttatgagtat actgacatta aacaacatta cccagcaatt
    14701 tggcgacaaa atcctctatg aaggtgtcga tctgcaatta aatgctggcg aacatcttgg
    14761 gctgattgga caaaacggag ccggtaaaag tacattgatt aaaatcatca ctggtgagat
    14821 cttaccagat gacggacaga tccattggca aaaaaatatt cacaaaggtt acctcgatca
    14881 atatgtggaa gtcgatgaaa cattaacaat cgaagaattt ctaaaaactg cgtacgcaaa
    14941 agaatacgaa aaggaagaaa aaattgcttt tctttaccaa acatatagcg agaccttcga
    15001 tgattccttg ctggaaaaag ctgggaaact ccaaacggaa ttagaccaag gagtgtttta
    15061 tcaaatcgac accttagttg ctgagatgag cagcgggttg gggatcgatg tcttaggttt
    15121 agattcactg ttgaaaaatc taagtggtgg acaacgttca aaggtgattt tagcaaaact
    15181 acttttagaa aacccagatg ttctgatctt agatgaacca accaatcact tagatgatca
    15241 gcatatccaa tggctgacgc agtttcttca ggattttgac ggtgcgtata tcgtgatttc
    15301 ccatgaccaa gaatttcttg atcagatcac cacccatatc gcagacatcg agtttggaaa
    15361 gattactaag tatactggcc atctaaaaca agcattgaaa caaaaggaac aaaatcgcga
    15421 aagctatttg cgccaatatc atgctcaaca aaaacaaatc gaaaagaccg aagcttatat
    15481 tcgaaaatac aaagccggtt cccgatcaac gatggcaaaa agccgacaaa aacaattaga
    15541 taaaatcgaa cggctgacgc ctccagcttc agcaagtaaa gctgtctttg attttccatt
    15601 ctcgcctatt gtcacgacat tagcagtgga aacaactgat ttagtcatcg gatatgatcg
    15661 accgctactt gcacccatca atttgaccat ccgctttggt gaaaatattg ccattcgagg
    15721 cttcaacggg attgggaaat ctactttatt aaaaacactg atcggtgaga ttgaaaaaat
    15781 aagtggcgat ttccacttcc cagataacac aaaaatcaat tacttttcac aagatctgta
    15841 ctgggaaaat ccattagaaa caccactaca atatttaagc agtcaatttc caaaagcgac
    15901 tatcaaagaa ttacgtcgcc aactagctaa agcagggtta gttaatcaat tagcaagtga
    15961 acctctcact acattaagtg gtggggaaca aactaaggtg aaacttgcac agttgacaat
    16021 gaaccaaggt aatttactga ttctcgatga accgacgaac catatcgatc aagaaacgaa
    16081 agaaagctta caagacggga tacagaaatt tccaggaaca gtaatcatcg tctctcatga
    16141 acaagaattt tatcaagatc ttgtgcaacg agtcattgag attgaaccaa aataaacgtt
    16201 tgattgaata gttgaaaaaa aggagctgtg acaaaagttt tgtcacaact ccttcttctg
    16261 aataaatggt ggccaattag ctgaccttcg gtattagctc aaaaaacggc aaaatatgag
    16321 gagctatttt tatttggcta ttcttattac ttgatgcaga acagcttttc acaacctctt
    16381 ttcgtctatt ttttattttc gagatttctc tttcctttaa aaacaactgt ttgaaagaag
    16441 aacgcacatc cttgaaaaac aagcccgcta accgttacac ctaaccagcc atactgctgc
    16501 cacattgtcg tccctagcaa tgaaccagcc gcacctccaa taaagtacat aaacataaat
    16561 acagtattat ttcggttgct tgctgcttca cctagccctt gtactcgtgt ttgattggcg
    16621 acttgaccaa attgattccc gacgtctaat aaaataatag ctaaaagcaa aatggccaaa
    16681 tggcttccgc caaccaataa agcaataaag cttattgttt gcataacaag tcccatatac
    16741 acgatttttc tttcagaata agaatctgcc agtcgtccca cgataggtgc agcaactgct
    16801 ccagacaaac caaaaatcgc taatatccct gcttcgaatg ttccccaatt gtaggcggga
    16861 ctgctgatat aaaaaatcaa cgtagaccaa aagatcgaaa atgttccaaa catgaaaaat
    16921 ccactgactg ccgcttctct taatagacgt tgagaagcaa ctaaatgcgg caaactttta
    16981 agtgaatcaa ggtatgaaac agctgtacta ctttgctgga cagaacgcgg cattttccag
    17041 taaagcagaa gtgtaagaat tccgactgca cataaagcaa ataaatagat catccgccaa
    17101 gaaaaaagac tggcaacaat accagatacc gtccgagaaa gcaaaatccc cacgagtaaa
    17161 ccgctaagca atgtacccat gaccttccct cttgcttttg gtcccgctag taccgcaccg
    17221 taaggaatga tgatttgcgg cacgatcgaa agtaatccta ttaaaaaaga agcgcatgca
    17281 aataacgtaa acgaaggcgc aaaaaaagcg gccaacagag agacggcaga taacgctgcc
    17341 attcgaatga tcaattttgg acgatccacc acgtccccta aaggaactaa aaacagtaac
    17401 cctaacgcat agcctaattg ggtcaacatc gtcaatatcc cgactgcact tgtgctaaca
    17461 gataaactag ctgctacttt tgttccaatc ggctggatgt aatacatatt ggcaacaaca
    17521 acgccacaag tgattgataa tagtaaaata agcgaagttg ttaaaggttt tttttcatta
    17581 ctcttcattg atttccccca tttcctatct tagtttacaa cttatggaaa agattggaaa
    17641 tagaaaaata caactattcg ccacacttat tatttgtgct tttgttttcc ttgttttttc
    17701 ttgcgatctt tataaatgaa gacaactaac cctactagca cagtaacact tgcaaccaac
    17761 gtgactgcag ttttgttata tccgtgaaac agagcttctc caccacacgc atacaacaca
    17821 gacgaaggga tgactccaat cacgatcgac aatatgattt gctgtagagg caatttcatc
    17881 gcatctgcag caaaattgac catagaagat ggaataaccg gtatcatata gcctaacatt
    17941 acacctattt ttggatgttt catttgtcgg attgcttgca cccaatgatt cgtttcggtt
    18001 ttcttatcta aaaattttaa gcgatgcata aggaaaattg cagtgatgtt accaagagca
    18061 ttacccaaaa tgttgatgat cgcacctgtc agtggtccaa agctcaaacc agcaatgact
    18121 ccaactactg aagtaggaat acctggaaca gcacacatca ccgctgtcaa tacgatcaag
    18181 agtatcgatg caaaagtccc atgcgaacga atatgtgcga gtagctctgc cttactcgct
    18241 ctcggattta aaaataattg gatgtctgtc tgatattcta aatacaaccg atagcccact
    18301 accagtatca gaatgattcc caacgagatc aatatcaatc ggattatttt ctttttcata
    18361 tttcctcctg aaaaaaatct atattcatta aataatagtt taacataaaa acaaaaaagc
    18421 accgaaatcc aacataggat ctcagtgctt ttaaatagta aaaattattt aacagttaca
    18481 gatgcgccaa cttcttcaag tttagctttc aattcttcag cttcttcttt agatacgcct
    18541 tctttaactg gtgcaggagc gccatcaact acagctttag cttctttcaa gcctaaacca
    18601 gttgcttcac gaactgcttt gataacttta actttttggt ctccagctgc agttaattct
    18661 acagtgaatt ctgtttgttc ttcagcagca gcagcgccac cagcagcagc tacagctaca
    18721 ggagcagcag cagatacgcc aaattcttct tcaatagctt taactaggtc gttaagttct
    18781 aggatagttg cttcttttaa ctcagcaacg atgttttcaa tgttcaatgc cattataatt
    18841 tcctccgttt aattgttctt ttttatttaa tgctttatgc ggcaattaag ctgcatctcc
    18901 atcttctttg tctgccactg ctttgacagc gtaagccacg ttgcggactg gcgcttgtag
    18961 tacagatagc aacatagata gaagtccttc gcgatttggc aattttgcca atgctgtgat
    19021 ttcttccaat gaagagactt taccttcgat gataccgcct ttgatttcta atgcttttgc
    19081 ttctttagca aattcgtcca tgattttcgc tggtgcgact acatcttcgt tactgaatgc
    19141 aacagctgta ggtccagtga atacttcgtc catgccttca aggccagctt ttttagctgc
    19201 acgtgaaaga atagaatttt tgataacttt catttctacg ttagcatcac gaagttgttt
    19261 acgtaaacgt gttacttcat caactgttaa tccgcggtag tcaacgacta ctactgatgc
    19321 agctgctgta aatttttcgc ttacttcatc aacgattgct gctttttttg cgataattgc
    19381 ttcactcatt tttgagtttc acctcctgaa ttttgatgga agagtttttt gcactgaaaa
    19441 ggcaagtcac ttttatagtt actttgctca accagtatta ttcatcacct attcacttcg
    19501 ttcatagtga ttctactcgc tttgctcgaa aggaaactcc tttcgctttg ctcaaccagt
    19561 attgttcatc atctcttcac ttcgttcata gtgattcaca ataaaaaact ctatgccacc
    19621 gcagacatag agggactatt gaatatattc aataaacgtc ctcggtagga aattaaggct
    19681 tgatgccacc tactgtcttc ggtacaagtt aattattaac ctcaactagc ttagcatgct
    19741 aaactagttg agtcaagaat tatttttcag ttggatcaat caacattcta atatttaatt
    19801 agaaagaagc ttggtcaaca tggataccag gtccaaatgt tgttgtgatc gttacatttt
    19861 tcatgtattg acctttagct gctgatggtt tagcttttag caatacatca tggattgctg
    19921 caaagttttc aactaatttt tcatcgtcaa atgaaacttt tccgattggt acatggatat
    19981 ttccagcttt gtccacacgg taagttactt taccagcttt cacttcgttt actgcttttg
    20041 ttacgtccat tgtaactgta ccagttttag ggtttggcat taagcctttt ggtcctaata
    20101 ctcgtcctag acgacctaca gtagccatca tatcaggtgt tgcaacaact acatcaaagt
    20161 caaaccatcc gttttggatt ttttgaacca tgtcgtcgtc acctacgaaa tctgcaccag
    20221 ctgcttcagc ttcttttgct ttgtctcctt tagcaaaaac taaaacagtt tgtgttttac
    20281 cagttccgtt tggtaataca acagcaccac ggatttgttg gtctgctttt ttagggtcta
    20341 cgttcaaacg gtatgcaact tcaactgttg catcaaattt tgcaatgttt gtttcttttg
    20401 ctaaagctac tgcttcggca acaggataaa ctttacttga atcaactttt tttaatgcat
    20461 cttgcatttt tttgctcttt ttagccattt tggttcctcc ttgattgtgg ttataacggt
    20521 tgtacctccc acgtgttcta tatctttcaa tatagagcca actgagaagc tgctgtctta
    20581 gggtgtatag attattctac agtgatcccc atgcttcgtg cagttccttc aaccatgcgc
    20641 attgctgctt caacgttagc tgcgtttagg tcttccattt ttaattcagc gatttctctt
    20701 acttgatcgc tagaaacttt cgcaacttta tttttgtttg gttcgcctga acctttttcg
    20761 attttagctg cttttttcaa tagaacagca gctggcggtg tttttgtaac gaatgtgaat
    20821 gaacggtctt cgtatacaga aataacaacc ggaatgatca aacctgcttg atcagctgta
    20881 cgagcgttga attcttttgt gaatcccatg atattgatac ccgcttgacc tagtgcagga
    20941 cctactggtg gagctggagt tgctttacct gcaggaattt gcaatttaac gattttttct
    21001 actttctttg ccacgagaca tacctcctta agtccgtgat gtggttaatt ggagatgcat
    21061 atttctcctc ccacgtttta tctgatctga aaaaatccag acaaataatg tgcaaaacaa
    21121 tgcacactgg tacagtatag caaatttatt tgttttttca agattttttt ctgctatttt
    21181 tacattttgc ttgaataaac gctaaattta ttctaaaaaa acaacaattt ttcgttctca
    21241 tttcttcttt tatgttacac ttgcgctagc atggttcaac agatgaaagc aaacaataac
    21301 acggtttttt agacccttgt acaaagattc ctacataaca aaatgaggtg gagaaaatga
    21361 aagatttaag gtatagaagt gtctttgata ttattggacc tgtgatgatt ggtccaagta
    21421 gctctcacac tgccggagct gctagaatcg ggaaaatcat acgtagtata tttggcgaac
    21481 agcctgattc tgtagatatt tatctatatg aatcatttgc aaaaacctat cgggggcacg
    21541 gaacagatat tgcattagtt ggcggcctgt tagggatgga acccgatgat gaacgtttag
    21601 ctgattcttt gaaattggca tatgaagccg gtatggaagt cttgtttgtt cctaagagtg
    21661 aaaaagccga acatccaaac tctgtaaaga tgcttctaaa aaaaggaaca cgaaagcttt
    21721 cagtcactgg catttcgatc ggcggtggca atatccagat ttctgaattg aatggattca
    21781 aaatttcttt aagcatgggt actcctacct tgatcgttgt ccaccaagat gttccaggta
    21841 tgattgctaa agtgactagt atactttctg agaccaatac gaatatcggc actatgacag
    21901 tgacacggga atcaaaaggt gaaaatgcca ttatgataat tgaagtagat gatcctcaag
    21961 tagaagaaac ggtaaaaaga ctcaaacagc tgccaaatat cgacagcgta aattatttcg
    22021 attaaggagc taaaaaaatg tttttatcga ttgaagaatt agtagatcaa gcaaaagctt
    22081 atccaagtgt agcagaattg atgattgcag ttgaaatgga aatgagtggc cgttcccgta
    22141 gtcacatcat cgagatcatg gaaaaaaacc tctcagtaat ggaacaatca atcaacgagg
    22201 gaaaagaagg tgttacctcc gtcacaggaa ttaccggtgg tgatgcaaaa aaattagatg
    22261 attatttaaa tgaggcagac tttttaagtg gagaaacaat cttaaaagct gtacgcaatg
    22321 ctgtagctgt taatgaagtc aatgcaaaaa tggggttgat ttgtgctaca ccgactgccg
    22381 gaagcgcggg tgttgttcct ggtgtgctga tggctgtccg agatcggctt gatttgacac
    22441 atgaacagca attggaattc ttatttaccg ctggcgcttt cgggttagtc atcgcaaaca
    22501 acgcttctat cagtggtgca gaagggggat gtcaagcaga agttggttct gcaagtgcaa
    22561 tggcagcagc agctttggtt tgtgcaaaaa atgggactcc tcatcaagca gcacaagcag
    22621 tagccattac attgaaaaat atgatgggac tgatttgcga tcccgttgca ggcttagttg
    22681 aagtcccgtg tgtcaaacgt aatgctttag gctcttctca agcttttatc tctgctgata
    22741 tggcattagc gggtatcgaa agtgtcattc cgccggatga agtcgttgct gcaatgtacc
    22801 aagtcggacg tcaaatgccg tcgattttca aagaaacagc agaaggtggc ttagctgtta
    22861 cgccaactgc taaacgactt gcggaagaga ttttaaataa gagggcgtga taaagctcct
    22921 taatttcgag tattaagaga ttagttatct tcataacaaa aaacgaagag aggactaatt
    22981 gcccttctct tcgtttttta ttaatccatc aatttcgtat tttttacttc atcgatcgtt
    23041 tttgttcctg caagttgcat cgtgatcgac aattctttgt tcaggtgttc aaaaacagat
    23101 gttactcctt ctgccccacc tagattcaac ccataaatca ctggtcggcc aatcgcaact
    23161 aagtcagcac cacttgccag agctttgaag acatgctctc ctcgacggac accagagtcg
    23221 aaaatcactg gtacacgttt gttgactact tcggcaattt ttggcaatac ttcaaaggac
    23281 gcaggacctc catctagttg acgtccacca tgattggata cccagatacc atctgcccct
    23341 gcagagatag cgattactgc atcttctggc gattgtattc cttttacgat aactggaaga
    23401 tgggtgattt ctttgattgt tttgatgtca tctggtgtca agccttgttt tgcagctgca
    23461 taaatttctg cgattccttt tccttcgcca ttaccacttg cactttgttc gctatacgca
    23521 gctaagttag gcattggtaa tggaaattgg aattgattga tgacgtcttc ttcacggtaa
    23581 ccaccgagcg ttgaatctgc cgttaaaatg atagctttag cccctgcttc tactgctttg
    23641 ttcaaaatga actcattgaa tctgtcgtct ttactcatat ataattggaa aaactgcggt
    23701 gcgcctggcg ctgcatcggc tgcgtctttg attgtcgtat ttgcataagt gctgatggaa
    23761 aagattgatc cagcagcagc tactccttta gctgtatcag cttccccttt ttcatgtgcc
    23821 aatccttggg ccgctgatgg cgcttggata atcggtgtat ctaattcaat tccgaaaaca
    23881 gatgtatgca aatcggctga gtcgattccg cgtaatatcc gtggcatgat tttctttgta
    23941 ttaaaagaag tcgtattttc tttcatcgtc cactcatctt cagaaccacc gcgaatatag
    24001 ccaaaagctc ctgcttccat gcgctctttt acacgacctt ctagttaggc aatgtttaca
    24061 atatcgattg gatgttcttc attacttgta tgataagtca tagtagatcc ttctttcatc
    24121 ttcttgatta catataaatc atactaaact tacaaaaagt aagcaaacat ttactctcag
    24181 atacttttat cgctattctt tccaaataaa tcgacttaca agctgatcta cttcttggtt
    24241 ttcatgaagc atactatgag aggcattttc acctgtcact actttttcct ctatttcatt
    24301 tccgcgttgc ttcaacaacg catagaccgc taatgcactg tttaacggta ctgttccatc
    24361 actcaagtct gtttcatcta gttgaccagc aatcaagaaa aatcgagtgg ttataggaat
    24421 attaccgatc atttgctggt aatcttgata gcggttagaa acgacagcag gcccctttgc
    24481 taactcatct gtcagagact gcgcactatc atcgacaaaa tcattaaaag gcgcgccaat
    24541 tgctgttaat gtctgaactt gtggcaatga cgtatcctgt ccatacgttc ctaaatagcg
    24601 tagtgcacta actcctccca tggaatgccc cactatgttg actttatcta tgccgtagtt
    24661 ttctttcaga tacaaaagac aagttttgat ccattctgct tgattccact cgttattttt
    24721 attatcttca aacaatactt ggaccatcgg attgtctgct tttccagaca aatctcctgt
    24781 tgcttgtatt tgtccatctg ttcctactgt taaaaccaat tcttttactg cttgctgatt
    24841 tttttccaaa cgccgcaaca tccctttaaa ggaattttct gtcccgctgt agccgtggaa
    24901 aaaaagtgtt ggaatagtgg ttttttctat cactttactg gattctgccg ttttttcttt
    24961 atcagaaact tcagtagtat ttatcttcgt cgtctggcaa ccacttaacg caaacaaact
    25021 gataaggact aaagctgctg cagttgcacg tttgcataaa tttttgattg gttctgtcat
    25081 acacctcttc ctttccatat agaaaaaaga aagacttgaa aggccaatca tatgatcgac
    25141 atttcaagtc tctttattaa tcgattttat ctacttgatc gaaatcaagt tctgtactgg
    25201 tttcacgtcc aaacatatcg atgttgactt tcaatttttc tttttctccg tcaacttctg
    25261 ttacttgtcc ttctaatcca gcaaatgcgc cttcgatgat tttcacgact tcgccaactt
    25321 ctacttctag atcattttgt cgcaggctca taccgataga acgcaagata tgattgactt
    25381 cttctggaag taatggtgct ggtttgcttc cggctccgtg tgaaccaacg aatcctgtca
    25441 caccaggtgt gttgcggaca acataccatg aagcgtctgt catgatcatt tcaacaagta
    25501 cgtatcctgg gaatgtttta tgaacgattt ctttttcttt cccatttttg acttcagttt
    25561 ctttttcttc aggaacaacg acacggaaaa taaagtcggc cattttcata ctttgcgcac
    25621 gagattcgat atttgctttt actttgtttt catatcctga atatgtatgc aagacatacc
    25681 agtttttttc aaaagattcc attgaaggct cctcatttca ttgtaaaata aaaaaacctc
    25741 agtacacccg aagtttttct ttcgcttcat tatagcacta aaagtacgtt gtggctagcg
    25801 agttatttta aaatccagcc aaataagctt tggatggctg tatccattac gtaaaacatc
    25861 aatccgaaaa taatagaagt ttcgatgact actaacgtat ctttacggag ttgttttctg
    25921 cttggccaag ttacttgttt gatttcatct tttacgcttt ttagaaattt catgagacaa
    25981 cctcccttgc tactgtttat ttcgtttctt tatgtaatgt atattgatta caatatttac
    26041 aaaacttatt gatttccaaa cgttcgccgc tttttccttc actgactgat tttgtgtagt
    26101 tgcgagaccc acaaacggaa caagcgagtg ctgctttttt ctttgccatt tacttctgcc
    26161 cccttgctga gaatcagact tatacttcat aacagtatca cgtaactttt tacttgtcaa
    26221 tttgtgaaat aaactatcta ttgttttgga taaaattatg atatgattaa tttgaaatta
    26281 agctaaaatg agacaattgg aggtatagcg atgctattaa aaacacttgt atataagaaa
    26341 aaagatctga caaccgttac agagactagt acattagaag aagcattaaa aattttagaa
    26401 acttccggat accgttgtgt gccgatctta gattcttcag gtaatatttt ccgtggaaac
    26461 atttacaaaa tgcatatcta tcgtcataaa gcaaatggcg gtgatatgac gcttcctgtc
    26521 actcatcttt tgaaaaacgc tacaaagttc atttacctgg atacttcttt cttcaaagta
    26581 ttctttacga tcaaagagct tccttatatt gctgtccttg acagtgaaaa tcatttttat
    26641 ggtattttga cacatagtac gttgcttaat atgctctctc aatcttggag cgtagaacaa
    26701 ggaagctatg tactgacaat tgcttccaca ggaaagcaag gagatttggc tgctattgca
    26761 aaaatcattt caaaacacag cagcattgcc agctgtatta ctcttgatat cgaaaaagac
    26821 gaatacattc gacgtacatt gatcacgcta ccggctaaca ctgaagcgaa agtatgtgaa
    26881 acgatcgtca accacttaga gaaaaagaat tataaagtgg tagaaattga aaatttgcaa
    26941 aaccaagaag cataatcaaa aagcgaaaaa agaaaacagc taatctgctt tcttttttcg
    27001 ctttttataa ttagacacgc ttttcaaaat caagcgcgcg atctaaataa tacatcaatg
    27061 gtgctgaaac agccatgacg ataaactcac ttaatgcaag cgtgccatag gttgtccaaa
    27121 atgcttggcc acctgttgga actaacatat aagcaatcag tcccatgctg atggtaaaga
    27181 agatcgtgtt caatactaaa cgcatcttta catttttcac tttgttttgc aataaagctg
    27241 tcaggcctaa agaaagcaat gtttggccgc caccaaaaac cacatccatg ataccaaaac
    27301 caaagaaggc attatagacg atgaccccgc ctaaaactcc ccaaagcaat ttacggttaa
    27361 agacaactaa atgattcaaa ctttctgaaa tacgaaactg gatcggccct gaggatacag
    27421 gcgcaactaa aatagttaac gctagataaa gagccatgat gatgccattt acaacgatta
    27481 cggataaccg ttgtctgctt ttcgtttttt gcattttctc tcctcttttc tcctagtttt
    27541 tttaacgcgg gatggttgat gaaccgcggt tatgtgaaac aaacaataat tataacagac
    27601 ttttcattta tttcaatccg tcttatctgc ttttaaaaaa gcaaaagctt aaacaatgta
    27661 aacgtttgct aaaaatcgtg ggatatggta taaagtaagt gtaaggggtc ataagaattc
    27721 ccagtaaagc agacgaagta ggatctgcaa gaacagaatt gttgtgaaac ttaaataaat
    27781 aaaaaaggat gtgggaattt gagcaacctc ttactagaga acggtctagc aagcagctga
    27841 agatctaagt ggacatgccg aaccttggaa cttctcctta cgtaagtaat ggatcatgcg
    27901 cttgctaggc ttttttgtgt ttttcggtct tcggaccttt ttcattttcc tagacttctg
    27961 ctataataac caagaattaa ttttgtaaca gatttttttg aaaaaacaag tttctttcat
    28021 tcaaaaaata gaaaaagctt gctaattatt ggttaaccag caaacaggag tattaaaata
    28081 tcttgtttta ttgaatagca ggaggaaatc atgaaattcc attatcatat attgatcaat
    28141 ccatcggcag gcagcggaaa tggctataaa gtagcagaaa gaattttacc ggtattaaaa
    28201 aataagcata ttgactatac acttcattat agtgaataca aaggacacga tgctgagatt
    28261 gctgaaactt tggcaaaaga aacattgatt ccttggatag aagaagaaca gaaaacaatc
    28321 gatactttcc cccttttgat catcgtagga ggtgacggca ccttacatca ggtacttgat
    28381 actttctatc agatggaagt agaattccca gttgcttata ttccagcagg ttctggagat
    28441 gattttgctc gaggggcagg ccttccaaaa aatcccaaaa aaggacttca gctgatttta
    28501 gcagctcaat caccagaaaa agtccacata atggcttatg aagaaaaaat aagtgaaaaa
    28561 aaaggaatag ctgttaataa tttcggtata ggattagatg cagctatcgt ccatgcgaca
    28621 aatcattctt ccacaaaaaa gcgattgaac aaatacaact taggatcatt ttcgtattta
    28681 ttctctattc tccgtgcttt attcactcaa aaaggatttc caattctggt agatgcagga
    28741 ggaaaacaat taagtttttc aaacgcattt ttatgtacag caacgaagca tccttttttt
    28801 ggcggaggaa tcgcgattgt tccaactgct gatgcgtcta aaccagtcat tgattttgtc
    28861 gtggtcgaac gaatcaattt ctttaaaatc ttgtggctca ttcttttact tatacagaaa
    28921 aaacaattga aatcgaaata tttccatcac tacacaacaa acagattacg tatcgtttca
    28981 acaacaccgc aacatggtca agaagatgga gaagaaatgg aaaaacgacc ttttgatatc
    29041 acaataacca caactaaaca gttgttttgg tgttaataaa agaggttttg tcagaagcta
    29101 catctggaac accgtttatt cggaaaaaag gactgtgaca cgacttttgt cacagtcctt
    29161 tttttactat gcagttaatt ctttgcccaa ttgggttgcc atcgatagtt tctttcttta
    29221 cccaaaataa attggatcat tgaaaacacc gcagcaaaag caactaagac acaaccattg
    29281 aaccaaaaag attctgcaaa tgtttgattg taactgagtg tttcactgat caagttatta
    29341 ttttgaacaa accaatatgt tggtgtgaat gccgctattt tgctaaccat ttctggcaag
    29401 tattctgctg gtacaaacac cccaccgata aagcaactgc ctgtgataaa aatatctttg
    29461 atcccaccgc tggtttctga gttttttacc aatgaagtga tacaagccga aaagctaatg
    29521 atcggcaata aaaacaagaa agtatttaaa atatgatacc ataccgttat atcccacgag
    29581 gtgtggatca ctaacagaac cattagaagt aagaagccac tgaacaacag cagcgagtaa
    29641 ctaatcaaac taaaggttac tttgcgattc aagtttcgtt tggtaatggg cgcactctgg
    29701 tttcgtttac tgatctcttt gcgattaaat gctagattga tgacgctgaa accgctaaaa
    29761 atggatagaa acaacccata agacaagaaa ttaaaggttg tgcccgtcaa cagctttttc
    29821 atccgctgac tatatcccgc acttagctca acttctccag taaccgagag tgccatttgt
    29881 gtctgttcca tagctgcatc gattgtttta tcttcttctt gaaacagaat cagtgtttgc
    29941 aaaaaggtgt tcacttgctg tgtaacaact gttttagaaa aggcatctgg gcgactctgt
    30001 atcttgatcg tcggcatctt tcctacttcg acttgttcgg taaagtctgc aggcagatgt
    30061 aagataatgt ttacacgatt aaaataaaga gcatcatcga tttctttttg tgaggttcca
    30121 tccaatgtaa cgactgtttc cttgtcattc aaatatttac gaaggccatc gactacaggc
    30181 gactcttctt cactaatcaa ggcgattttc gcatcttgca gagaggtatt ttcaacatca
    30241 atattttgcg caaaaaagaa tgtggcaaca acagtgataa ttacacctat cagtaaattc
    30301 gatttatttg ctttaagcaa tgtccaaatc gttttataaa gcgtcataac gcacccctct
    30361 ttccatccaa gcatttccta acgcaaaaag gaaaataaat ccgatcaacc atagaagatt
    30421 ggtataaaaa ctagccaaat cagtgtagaa ataaagttta tacaaacttt cgctgatcaa
    30481 gttgacgaga tttaattgac caacaatcgg aatatgaaga tcaatccaat acttcacttg
    30541 ttccgacccc atcataccag caaaaaagct catcgcaatc gtcactgaaa tcaagatgct
    30601 atccttttgc accagattca tttttggtat caaattacca accaatgtac ctaacaaaat
    30661 cgcacacaac gaccccagag cggtaacaat catcaaccat tgccaacgtt caccaaagtc
    30721 tacttgatag ataaaacgaa agacagctaa cataatataa atctctgtca aaacaatcgt
    30781 aaagcttgct gccaaattag cgattgaaac taacgattgc tttctaggaa tcaaggacaa
    30841 gcgaatacca tttgacgatt gatttgcttg ctgatcctgt acattgcgga ccccccacat
    30901 cgtcccgtag agaatcgcca ttccaatcag agtgaagaaa taaaaacttt tgaaactcat
    30961 attacgacct gcatgaactt cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg
    31021 cgcgagatca acgttctcca cttctgccac tttactcgaa attataccaa tacgctgtaa
    31081 aaattgatcc atgacgtttt tcatcaacgt ctgactcatt cctgcttgac ccacatggag
    31141 cgaaacagat gaaccatcaa tgagataaaa accagccaaa tcatctgcat ttagcttttc
    31201 attcgcttct acttccgata gttcctgtac ttcaaataat ggtttatcat tgacttcgac
    31261 ttgttctaag actgtggtaa aattttccga aagcgctgtt tgatccgctt tgattacaat
    31321 tccagtatta atcgtttcga gattgccacc ttgatcaatc tcgccaaagg ccgccataaa
    31381 catcaatcct aaaagaattg gaaaggccaa tgtccaaaac aacatggatt tatttcgcag
    31441 cagtattttt gtgcgatatt tataaaaatg ccaaaacatt atttcccctc ctctagtctc
    31501 tcaaatcctt gccagtaatc tctaaaaaga catcattcaa ggacgcttct ttggttgcta
    31561 tttttccgta actgatcttt tcttcttgta agattgtaag aatcggcaac aatactgtat
    31621 ttagttcagt agttgttact tggagctgac cttttttata agcgattcca tcagaagcta
    31681 ttgttcgctg acaacacgtt ttctgggagt aatgggatgt cgatcgtcaa acgatccgcc
    31741 gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg tccttgatcc
    31801 atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt tgtatagaca
    31861 atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt ccgactttgg
    31921 gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg ggcaatccca
    31981 catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg aaacgaaaca
    32041 tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct tttctctttt
    32101 tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag ttcgtcaaag
    32161 accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt ttcaggagtc
    32221 attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga aagcatacag
    32281 ttgatggcag tcgatttccc actgccgttt ggtcctaaca gacctaagac ttctccttct
    32341 tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt gactaattta
    32401 tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc tgactataat
    32461 aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat ttgtcacaat
    32521 gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc tactggttat
    32581 ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct atttattgat
    32641 cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt ggattttcca
    32701 agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc tgccagcgac
    32761 gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt taggtttttt
    32821 gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg taattttgct
    32881 gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg agaaaaagta
    32941 cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac aaaataaagc
    33001 attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac ggaaccgaat
    33061 tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt tacaaattgg
    33121 tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa atttaagtgc
    33181 caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc atgagacatc
    33241 gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt ctgttacgat
    33301 tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct taatggtcat
    33361 caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg ttttacattt
    33421 tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca gcccacaaaa
    33481 aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag aacaaaaggg
    33541 acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt taccaaggga
    33601 gggacaccaa tgataacagt agtcattatt gagatgatcc atttgtgacg aaatcgttac
    33661 aaaccatttt agaaagcacg aaagagatcc gtgtgttagg cattggtcat tgtgccaagg
    33721 atgcgctagt cttatacgaa acccatcgtc ccgatgtttt gctgacggac atccgcatgc
    33781 ctaaacaaac gggcatcgat gctgccaaag aaattttggc taaatttccc aaagccatcc
    33841 ttttgctttt gacgacattc aaagatgaag aatacatccg cgaagcattt tccatcggtg
    33901 tcaaagggta tttaatcaaa cagaacttgc aagcgattat tccttctgtc aaatccgctt
    33961 ataatggaca ggccgtattt ggtaatgaaa tcatcgagac tttcactcaa atgatgaaga
    34021 ataaaccgac gattgatagt catgcttatg cttcttttag tgaacgtgag cttgcaatca
    34081 tcaaagaagt agctgccggg aaaaacaata aagaaattgc agatgccttg tacttgagtg
    34141 acggcactgt tcgcaattac atcagccaat tactagaaaa gctagattta cgggatcgca
    34201 cgcaattggc tatctatttt tatcagcatt tgcaataagt cttatctaac acaaaaaaac
    34261 ctgaatgatg ctccttcgca tcattcaggt ttttttcaca tcttctattc aattgtcccg
    34321 caaaccattt cctcacgatt gaaagaatca aggattcgca gggctgccag gattcgttgg
    34381 attgttcggc gtgttaggcg gaaaaatagg cgccttgtct aacgtttttt ctagttttga
    34441 ttcttcacga tcgtctcgtt ctacatcagg actttcattc aatttgaatt ctttttccat
    34501 ctcatctaac tttttaaagt cattgccaga ttcttttgct aatttcgcta atttatcttc
    34561 ggtttcttta gccataaagt aacgcccccc ttttgatttc agcttagcat atcacactga
    34621 aaaaacaaaa gaaaacgcta accaacgatc atttttagct atttctcatc tttaaaatca
    34681 agcatttttt gccggtataa aatcgtaggg agaactgttt tccataccaa tcatcggatc
    34741 tactaactgg ttatcgatca cttctgctgt aagaacgaaa tctttagaaa cagcaggttc
    34801 tacctcttct ttcttttctt ctccaacaaa tgaagaaaac agatctggtt ccactgtttc
    34861 cattacttgg tttttcgttt cattcgtccc actgtcatct tctacaggta gcttttcttt
    34921 tacaacccgt ggctcttttg tatagttccc atcttcaaat ggatcatttc cgaccaaaga
    34981 atcttcataa gctgctagtt tggtccggag agtgatattc attttatcat tatccatgat
    35041 ccttgatttc aacgtagtca tccggcttgc tgattcattc gctacacaag tgacataggc
    35101 ttgctcttcc cctaacaaaa tcaacagttc cttactgcga gtcactgccg tgtagagcaa
    35161 attacgctga agcatccgct gatattgatg gaccatgggc aaaataacca tcttgaattc
    35221 actgccttgt gatttatgga ttgagcaaca ataagaaaga gtgattttat tccattcgtt
    35281 tcttttataa gtgacctcat ttgcatcaaa ctggataact aattcatcca ctttatcttc
    35341 agaatctttt gccaagatga tacccgtgat ttctcccata tctccattga atacattcaa
    35401 ttcgggagta ttgactaatt ggaggacttt gtctcctatc cgataaacgg tgtcgttcca
    35461 atgtacttcc ttttttccag gtttccctgg attgaatatc tcctgcatca ttttatttaa
    35521 cgcatcgatc cctgcaggtc cccgatacat tggtgctaat acttggatat cttgtgcacg
    35581 gaatccctta tttttcgctt tttccacaat tttagagatc aagggttcaa tttgataagc
    35641 atcacttcgg aaataggaac gatctttttg attctttgtg aaatcttttg gcagacgtcc
    35701 ttctttgatc tcatgggcaa gaggaataat actagaacca tcaccttgcc gataaatctg
    35761 attgagttcc attttgggaa tttgttcgat ttccaaaaga tcatggagta cttgaccagg
    35821 accgacagag ggaagctgat ctttgtctcc tactaagata acttgcatat tcgtcggaat
    35881 cgctttgaat aatgtgttag ccagccatgt atcgaccata gacatctcat caacaatcaa
    35941 aagtccgcct tctaattctt tcgtactgac tgaaggtgtt ttttcccgtc cattcagccc
    36001 cagaagacga tggatcgtac tggcaggcag acctgtcgtt tcgttcattc gtttagctgc
    36061 tcggccagtt ggtgctgcaa gtagaatagg aaaaatttct tgggtatatt ttgaaggatc
    36121 caattccagt ccgttcaact ctgcaaaaag attgacgatt ccgttgatca cagtagtttt
    36181 ccccgtacct ggtcctcctg ttaaaataaa tagaggggaa cggattgctt cagcgatagc
    36241 ttgttcttgc gaaggtccat actgtatgcc gaatattttt tcgatacgcc gaatattctt
    36301 ctgcacttct ttttcaggat aatggatttc ttttttacga ctgagaaggc gctggatcga
    36361 attaccgatc ccccattcag aaaagtacaa actgttttcg taaagattag tctcttcttg
    36421 ttgtatcttt ccttcttcaa caagttggat aattacagta gcaacctgat caggctggat
    36481 ttcaattggg cgacttgttt ctaaaatacg gatcgtttgc tgaagcaatg tctccgccgg
    36541 tacgtatgta tctcctgttt gaactgcatg ctgaaaaatc tgatgcaaga tagctgctcg
    36601 aattcttctc ggtgaatctg ctgcaatacc gatttgttcg gctagattat ctgcacgttt
    36661 gaacccgatt ccttctatgt cttcgactag ctggtacgga ttttcttcga tgatatccaa
    36721 agactcgttc ttataggcct ggtagatact gaaagctaac tgactaccga acccgtaacg
    36781 attcagtccc atgatcactt gatccatccc gtggtttagc cgaatcgtct cagcaagcat
    36841 ttctcttttt ggcttcgtca aaccggagat ttgttttaat accgaaggat cagcaatgat
    36901 tttttcgata gcatcttctc ccagcaattc tacaattttt tctgctgttt tcttcccgat
    36961 tcctgggaat ttttcgctgg aaagatacgc tacgatccca tttgcagttg ttggcttttc
    37021 ttgctcatat ctttcgagtt taagctgcct tccgtattta ggatgatcca ctaattctcc
    37081 ataaaaacga taagtctcat tttcttgaat atcgccgaaa cttccggtga ctacaatctc
    37141 tttttcacgg taatcagctg tggtttcact gatgtgtacc aaaagcactt tgtaaaaatt
    37201 actaggattt tgaaaaaaaa tcgcggcaac tgtaccaacg aaataaggca tttcttctgg
    37261 ctgcatcgga agccgcctcc ttcttctata gtaaaggttc tggtgctgtt tcagttccta
    37321 aaaaatctgt ttggttccat actttcagtt catatggcaa taatctgcaa ggctcttcag
    37381 tctctaaaat cgtcaaacta ctattgaaca atccacccat ttcacgcaac tcacttagct
    37441 cttttcctgt catccattga atggctgcag ttaaagaagc tccatgtcct acgaataaaa
    37501 gtgggccatc tttatgttgg gcaaccgcat ctgctacaac agtttcgata cgtgtgattg
    37561 ccgattcaat cgtttctccg tgaaaaggag aaggatcata cagatctaaa tgattacgca
    37621 aattcggcag attatccgga tatttctggt acatttcatc gatcgactga ccttctaaag
    37681 tacccagccc tagttctctt agcttatctg tataaaaaat ctctaccgga cgagcaagct
    37741 ctttgttgat accttctgct gtatcacgtg ctcgctttgc tgtactggaa taaattgctt
    37801 taaaaggaac atattttacc tttttcccaa atgctttgat ttcttcgtaa ctttgtggca
    37861 aaagcggaga atcgccattg ctcccttgaa aacgtctttc ctgattccat tgtgtcttcc
    37921 catgtcgggt aaaatatagt tccatacgca gcaaactcct gtctttcttt tattttactc
    37981 tataataaac tgtctaatcc agcttgtcat tttcatagtg atgcgtcaat tctaaattag
    38041 ggaatccttg ctggcgaagt gcttcataca ctatcattgc agctgtattc gataaattca
    38101 atgagcggac atgctcatca ttcataggga tgcgtaaaca cttttcttcg ttttcacgca
    38161 taaatgtttc tggcaaaccg gttgtttctt ttccaaataa aaagaagtgc tcttctccat
    38221 cagcaaaatt ttcgtcacta tatactttat gggcaaattt tgagatcaaa tgtagtttac
    38281 gattacctaa aaatgttaaa aaagcttcta aattactgtg gtaagtaata tttacatcat
    38341 tccaataatc caaccctgcc cgttttagat gtttgtcatc agttgaaaaa cctagtggct
    38401 cgattaaatg aagcggagta ttcgtggctg cacacgtacg ggcaatattt ccagtgtttg
    38461 cagggattaa tggttcaaat aaaacgatat gattcatcag gatcattcct ctcatattta
    38521 tgttcgtttc tattttctga caatttgatt tttgcataaa aaaaaacgta tcaactcggt
    38581 gtcagtcact gcgagtcgtt acgttagtga agctcttctc actaactttt atcagaacag
    38641 gaacctgata aaaaccaatg aaaaaacaac ttgttgctaa tataacaccc tcaacttttt
    38701 tttgcaacct ctactcatta aaaactgcta gaaaaacgtt ttctcttagt tccttttaat
    38761 aattctatgt caattgtaat cttcttcaaa ggattcttat ataagtctgc tttgattcgc
    38821 tcttctacct gccattctaa aggtgacatg gccaaaagat ctttttgatt ttcctctggt
    38881 atctcaaatt cataggttac acgctgacgt tcagttaatt tcattgcttc agcaaacttg
    38941 gctactacac gttcattgga ataactctgt ttttcaggtt gattcggata aaaagcctga
    39001 cgtagttcct ttaaataatt ttcttgtggc accaccttca acacttctcc atcttctgag
    39061 agtacacgtt ggaattcttg gtaatgagaa ggcgaaaaga tatttaaaac cgcgttgaat
    39121 gtttgatcag caaacggcaa attggtcaaa tcagctacac accaaaatgc ttcggcagat
    39181 tgttctgtag ctgcgtagac tccttctttt gaaatatcaa atccaacagc attttgaatc
    39241 tttttcaatt ttgaaagttc attcaagaag ctaccttctc cgcagccaac atctaatagc
    39301 gagacttctt caggtaaatg ctgcgcaata gtctcaataa cgggctgata catgccagat
    39361 tggatcattc tacgccttgg aacgaacatt ccttgatcgt attctgtttt aaccgcatgg
    39421 gataaaaaat aaagtgttcc ttttttggaa aggtcaaaac ggtgattatt tttgcatatc
    39481 aagccattat cagacttttt tacttcttct tggcaaaccg gacagcggaa cagctgatga
    39541 ttttctgcca aaaactggcg tcctcggtca atttttttta acatagttcc ccctcatttc
    39601 tgttttatct taccgttaga tgctcacttt ggcaactgct aaaaatatga tacaatgcct
    39661 agaagaaagt gaaagataaa ggagaagatt agatgatcaa agaattttgc gctgaaaact
    39721 acacatctat tcctctagca attgctagag gagccgatcg gatcgaattg tgtgacaact
    39781 tagcggtcgg tggcacaacc ccgagtactg gtgtgatcga agaaacgtta agttatgctg
    39841 gggaaaaaga tatccccgta atgacgatta ttcgtcctag agggggcgac ttcgtctata
    39901 acgatatcga attgaaaatc atgcatactg atttgattga agcaaagaaa ttagggacgg
    39961 atggcgtagt atttggttgt cttactcctt caggctggct agacgaagag gccttagaac
    40021 tattgatcga aacagcagaa gggttgcaga tcaccttcca tatggctttt gacagtattc
    40081 ctgaagatcg gcaattcgaa gcaatcgatt ggctggtaga acatggtgtt gaccgtatct
    40141 tgactcatgg cggtcctgct gacaaaacta ttgtagaaaa tttcccacgg cttaaagaat
    40201 tgattgccta tacagatggc cgcttaatca ttttgcctgg tggtggtgtc actgcagaga
    40261 atgcagcaca tgtagctaaa gaattgaacg taagcgaagt acacggaacc aaaatcgtgg
    40321 atcttcagtt ataatcttat aaacaaaaaa acatttgcaa caggcataat gcttattgca
    40381 aatgtttttg tttttttaca taaaatagcc cataaataaa gaagcaatat tgaaataaat
    40441 cagtacactc gtcaaatcgc tcaaagtcgt gataaaggga ccgcttgcta ctgctggatc
    40501 aaatcctaga cgatccatta gcatcgggat taaactacct gcaagattcg ccactgtgat
    40561 tgcaaatagc atagccatcc ctatgacaaa ccccaagata aaattatgtt tccaaaaacc
    40621 tacgattaga aaaatcgtga tccccgtaac agctcctgtg acaacgccgg tcaagacttc
    40681 agccaaaaca agtcgaacaa atccatgatc tttctcatca gaaatcgcaa gtcgacgaac
    40741 agccacagct agcgactggg ttccagcatt accggcagtt cctgtaatca gcgaaatgaa
    40801 gactgctaaa atactcgctt cacttaccaa gtcttcatag tgactgatta aagaagcggt
    40861 cgacatacct aaaaacaaca gagtgatcag ccaaggcaaa cgtcgagacg ctgcctttac
    40921 tggattttca ttaatttctt ccacattgac cccggcaagt cctgaataat cgctagcagc
    40981 ttcatcatca ataacgtcga tgatatcatc gactgtgacg attcctaaca aatgatcatc
    41041 ataatcggta acaggaagag ctaagaagtc ataatctcta aatgtttgtg ccacatcttc
    41101 ttggtcgtcg cctacatgaa ctgaaagcac tcgttcgctc atcagatcag aaatcattgc
    41161 gtcgtcatca ttcacgatta ggtcgcgcag agaaataacc cctactagct gaccttcttg
    41221 attaacgaca tagatgtaat agattgtttc agctacatct gcttcgttct tcagcacgta
    41281 catcgcagag cgaacagttt gattggctac gatcgaaaca aattcagttg tcatgatcgc
    41341 gccagccgtt tcatcctcgt aatggagaag ttccttgatt tcactggcat cgtctgccga
    41401 catcaaactt aaatatttag caatttgctt tttatccagc gtattaagca agtcaacagc
    41461 attatctgta tacatctcag aaagcatatc agctgcatag ctaggacgca tttcatctag
    41521 gtattctttc atatgttcgt tgtcttcttc aatcacatcg aacatatccg ctaattcttt
    41581 aggcgacaga taggaataga tcagctgcct gttctcttct gtaagagatt ggtagaattg
    41641 cccctgctca tagatatgca tctctaaaaa actgtttctg aatgcctgaa tattattttc
    41701 ccttaaatct tgagtcaact gggcaaatct ttcctctaat tcttgatttt catccaaacc
    41761 aatctcacct cgccttattc ttcaaaccgt ctcacaactg cctccatgtc tgcggctaaa
    41821 ggctgcttga actccagcaa ctcttctgta aatggatggt aaaaacgcag atcataacaa
    41881 tgaagggctt gtctttttat tcccagatcc attcgcccgc catacatatc atcaccgagc
    41941 agggcacagc caattgcgga aaaatgaact cttatttgat gtgtccttcc tgtatgcagc
    42001 tggatatcaa ctaacgcgat ttctgttagt cgcttctcta tccagtactc agtctgtgcc
    42061 gaacgtcctt catcagagac cattcgcttc aatagcgaag aataatctcg ggcgatcggc
    42121 aaatcgatat gtccatgtgc cgataaatct gaaagccttc cactgactaa ggcctgatat
    42181 tttttcacaa acttcttttc acgaagttgc atatctaatt ttgcgtgcgc aaatccatgc
    42241 tttgcaaaaa gcatcaaacc agatgtatcc cgatctagtc gcgtcaccac atggattact
    42301 tggttttcgt aaccttgttt tttataataa gcctttacac gattagccat tgtcccattt
    42361 ggatggtatt gggcaggaat agacgaaacg cctgctggtt tatttactac cagaacgtgt
    42421 tcatcttcat acacgatatc gatcggtgtt tcatccaaaa gtacggtttc atgttctcct
    42481 tcagcaggaa tcacaatggt taccttatca tccttagcta gagaaaacaa gacattttct
    42541 acttgatcat taactttgat ttgaccgcct tgaaatttta ttttcgctaa cagacccttt
    42601 gagatccctt tttcttttaa aaaatatttt acttgctgag aatgatcttt ctcataatcc
    42661 catgtaaact gcatatcaat cctcactaat aaaggcatct ttcacacgat gccaaaaatg
    42721 catatgtcga taagaagcaa aatggatccg ttcttctgct attcgataat aaattgagcg
    42781 gatatttgct tttgcgacat tcaattgatc aaccgttaca agataatctt ctgtattttc
    42841 caattttatt tctagccatt catcatgggc aatcacgatc ggagaaccca aagtacgaaa
    42901 cactcgatta ttcagtgaag caatctctgc caactgaaag gcattgatac taggatggat
    42961 cacagcaccg ccgacacttt tattatatgc tgtcgaacca gtaggcgtag aaatcgtcaa
    43021 tccatctcct ctaaaacttt caaacagctc gtttttgatg tatacatcgg ctaccatcgt
    43081 acgattggcc cgtttaatcg ttgattcatt cagtgctaaa aaatgctggt ccggtgtttc
    43141 atccaaataa ctgatccgaa catccagcaa gggataacta acactttgtt cccggttcgt
    43201 tcgtaagctg tctactagtt cttccagctc gtaatcccgc caatccgtgt aaaaccctaa
    43261 atgacctgta tgcacgccca agaaacgcac atcattcaga caatgactgt agtgatggaa
    43321 cgccgacaat aatgtgccgt ctccgccgac tgagatgacc aattctggct catgatcatc
    43381 tcgttgaatc cctgcttgtt ctaaaagtgc gttcaaacgt tctgtcactt ctatagtctt
    43441 tgcttcttta ttatgaacga tcgctacttt catcccctgc ctcctctctt tcgctttctt
    43501 tcttcaaaag cgttcgatag tagacatcat ctgattttcc tcttccatgg gagaataacc
    43561 gctgtgcttc ttggatttct tcacggatac tggacatctc ttcatccaac tgataggcag
    43621 cttctgccgc acgctgaagt cgttcactca tttcttctgg aaaaacgcct tgatatttat
    43681 aattcagcga atgctcaatc gttgcccaga aattcatcgc aagagtccga atctggattt
    43741 ccgccaaaat tttcttctca ccgctgatca gctgtacagg atactcgatc accagatgat
    43801 aagaacggta gccacttgct tttttatttg tgatgtaatc tcgttcttgg agaactttca
    43861 tgtcttttct tttacggatg atctcaacaa cttgatggat atcttctaca aactgacaca
    43921 tgatccttaa cccggcaata tcagacattt cttcttctaa acgtcccatt ggaatatgcc
    43981 gtaattgtga ttttgttaca atactatcga ctggcttgac tctcccagtg acaaattcaa
    44041 tcgggacatg tcgattttgt tcacggaact gtttgcggat cccccgcagt ttcactttca
    44101 attctgatac agtttgttca tatggtgcta aaaatgcttc ccaatctcgt tccatagctg
    44161 atacctctac ttcattaaca ttcattgttt attctaccat atatgcaaag ttttttcgta
    44221 ctcttgattt atgcattgac caaaaaaaga aaattaggca aaatagacaa aaaggagtcg
    44281 atcaaatgac acaaatgctt gaaatcgaat ataaatctat gctgacaaag caagagtacg
    44341 agatattcat cgctcactat caattaacag ataaagattt ccgtgtacag actaatattt
    44401 atttcgatac cgttgatgaa cagctgaaga aacaaaattg tggcctacgg atccgtttcg
    44461 ttggtcatca agctgaatat acattaaaaa cacctgctga aaaagggcgt ctcgaaacaa
    44521 cggatacctt tataacagaa gaagcagaag catttatctt cgaaaaacgt ctgcctcgtt
    44581 caggcgcagt ttttcaaaaa ctttctgatt tgaatatcga tcctgcttca ttaatccaaa
    44641 ccggaaagct tacaacaaaa cgggctgaat tccctattga agaaggactt ttagctattg
    44701 atgaaagctg gggagacaat ctacacgatt ttgaactgga actagaggta ggggatgcat
    44761 ctgtgggaaa aattgctttt ataaattttt taaaacgttt ttctttacct tatcgtcctg
    44821 ctaagaacaa gatccaacgt atgcttgaag ctaaaaatta gaaacctttc attttcacaa
    44881 ggcacttgag ttcatttttt tacttttttc tgttaagtta taggatgtga aagaatctag
    44941 tcactagttg tatagaatct attttggggg agattcatat gatcgaaatc tatttatttg
    45001 ttaatccttt aggggaatac tgtttcgaaa tggaacaaca gttattacaa tttatcgaag
    45061 aagagtacgg gacaccatca aaagaaaaga tgcaatttcg ctttcttcct ttggtcaatt
    45121 tgcagacaat tggggatgtc atgcaacaaa aaggaatttc tcagaacaat cttgaggaac
    45181 gtaatcactt attctcaatg acttattctg cagcattgga ctgcaaagcg gcgcagtttc
    45241 aaggaaagaa aaaaggccgc cgttttttga tcaaattgca agaagcagtc ggctgtaatg
    45301 acataccata ttctcaagaa cttgctgaat ccatatttga agaaatcggc ggcgatttga
    45361 atatgtttaa agaagatcgt cagtctgact tcgttaaaga agtattcttt tctgatcaaa
    45421 aaattgctcg ggaaatgggc atcacaaaac acccttctgc agtcgtttac aattatgcct
    45481 gcgaccgtga ttttggtgtt ttagtagaag taaaaggtac aagtagcctt tggagaatca
    45541 cagagctgtg taaaacaaca gataactcat atcaagtttt taatggtgaa catttacata
    45601 caaatgattc tcagttccat tcaagaacta gccatttaca cttgctttct aattaaaaaa
    45661 atacgtcgac tcccttacaa gagagccgac gtattttttt cgtttctatt cgagaaaatc
    45721 aaaaatagaa agccttataa acgatcgatc aattcttcta attcatttag acgttgttca
    45781 aacatcgaca ttgcatcttc aatatatgca gcctgtgtca tatcaactcc tgcttttttc
    45841 attacttcca caggataatc gctgttacct gcttttaggt aagccaagta gttctctagt
    45901 gcctctggtt cctggtttag tattttcttc gccagtgctg aagcagcaga aaagccagtc
    45961 gagtattgga aaacatagta attataataa aaatgcggta tccgtgacca ttcgaattta
    46021 atttctgggt cttcttcgac tgctggacca taatattttg cattcaattt accataacta
    46081 tcacttaggt attcactagt caatggcaca cctttttcat cttccgtatg catgaaatgc
    46141 tcgaattcag cgaattgtgt ctggcggaaa actgttcctt taaacccatc caagtaatgg
    46201 ttgagtacat aagcccgtac acgagggtct ttttctgttt ccaataaata ctccgttagg
    46261 atattttcat tcgttgtcga agcaatttct gctaaaaaga tggagtagtc gccgtacaca
    46321 taaggctggt tcgaacgagt gaaataacta tgaacactat gtcccatttc gtggacaagc
    46381 gtaaatagct gatccagcgt atcatgccaa ttcaataaaa tatatggatt tgtgtcatag
    46441 cttcccgaag aataagcatc gcttcgtttc cctttatttt cgacaacatc gatccaacgt
    46501 tcagagaatg ctttttctac gatggccatg tattcttcac ccattggttt cagtgcttct
    46561 aaagcttttt cttttgcttc ttcgtacgta aaggtaattg gagcttcccc taagaccggt
    46621 gtataaagat catacatgtg cagtttttcc acttctaata aacgtttccg taattccatg
    46681 tatcgatgta acaaaggcaa atgcttgttt accacgtcta ccaaagtatc gtatacactt
    46741 tcaggaatat gattattgct caaagacgct tctctggcag agctgtaatt acggactttc
    46801 gctttaaaat tatgtccttt tatatgtgtg cctaaagtag aagcaaatgt atttctaaat
    46861 tgttcgtaaa cactgtacaa tcccttaaat gctgcttcgc gcacccttcg atccgtgctt
    46921 tctagcaact gaccatacac gccatgagat aattggacta tttcaccatt ttctccttca
    46981 atcgttggaa aaactagatc tgcattattc aaaacagcaa atgtatcact tgaagcatca
    47041 aagatttcac ctgctccagc aagaagagat tcctgctcag cagataagac atgggctcga
    47101 ttatctacta tttgctggat ataatggcga tagacttcca attttggttc ttctttgaaa
    47161 tattgccaaa tctggtcatc tgacaattgc aatatttctg gttcaaacca ggaaacggct
    47221 tcactaactt tcgaaaacag actgcttgct cttgcataaa gcgcttggta atctgtattt
    47281 ccagtgtctt gatcgttttt aaggtgcgca tatacataaa tgacttcagt ttggcgataa
    47341 accctcaata cgaattcaat cgcatttaaa aattgagaag cgccttgatc aagtgttcct
    47401 ttgtgttttt cagattgttt taactcttct gataattcca aatatttctc atcaaactct
    47461 tggtcgcttg aaaagatctt ggtcaagtcc caagttaaat tttcaggcaa ttcttctcgt
    47521 tttggcaact gctttacttc catgaaatcc actccttttc ttcccttagc tctatctatc
    47581 aaaatagatc atagtctaac atccacatgt cacagcatct aacttttcta ctcattcatg
    47641 tgtctgcttc catcaatttt agcataacta aatcttgttt tacatagaaa accgattcct
    47701 agcctatttt tatacttact attttttcaa agaagacaag tgtatgcatt cgttaaaaaa
    47761 ctgctgatag accatctctt catcaatcat aggaaaaagc cattcccttt tatgttcttt
    47821 tatttggcaa agccatgagt tgaaatcagg aactttctgg ttttccaagg cttcttcgta
    47881 caacattcga taaagaaaaa cctgttcttg taaataaaag aaaaaccgac ttttttgata
    47941 gatccacgat gatagatgca aaagatgttt atgacgcaag tagcattgtt cttgtatgga
    48001 tactgtattc ttttgcttgt gaataagttg gcgtgtcagc catttcttcc catcttcact
    48061 cactttttct atttttttca tttggttcat tttaatttcg ttactagtga atatttcttt
    48121 aaggtcccta ctagaaaaag tccagcagct actgcaaaag gaaatatgcc ctgatacagt
    48181 ctctgtaata tgatgataca aataaagctt ttgtttcttg agatcagctt tccagcaatg
    48241 cactcctctt tttctattat agtagcagca acttttttct atcaatgaga attgcgattg
    48301 tccaagaaaa gaattaccaa gtatccacca tacttggtaa ctgttattca aataattttg
    48361 ggttctttca ataaatctct ggtgagacag tctagaacat tggatttcta ttgctagatt
    48421 cgccaacaaa atatccggcc tttgccgcaa cctcggcaaa taggcttcca aaaatactgg
    48481 ttctactgac tgtcccagcc aatccatgaa gcattccttt aactgtaagt gctcttttga
    48541 ttctctttca ctaaagaccg aacagttatc tgatttttga tgggcaaaat gagcacattt
    48601 tttctttccc tttttcaaaa tcactctttt tcgacagcca cgacaataat atttctttcc
    48661 tttttctgca tgtttagcaa aacagcactt attatattca ttcattgcaa gcagcatttt
    48721 tctccctctg aacttattta cgtctaagaa tttatttaca cgaaaaaaga gggcgtgaaa
    48781 aagctattct gcatcaagta ataagaacag ctaaacaaaa atagttccca atatttttcg
    48841 tttggttggg cttgttaccg cagatgcaag cttttgaaca catttatacg aaaaaaagac
    48901 tgcgacacaa tttttgtcac agtctcttac tataatgctg atttatacaa aataaaattt
    48961 cgttcgctcg atagcattac gcgccatcag acattgtcca tattcattca atgtttctgg
    49021 tgtacgatcg ctcttttcag caaactccaa aattcgagca atctgattct ctacatccgc
    49081 ttggttgaga ttttccatcc agaagtgaac gactaggtaa tagcgctctt cgaataaata
    49141 caaatcagtc caagcagact ctagttcgac atctgttgca agttggatca cttgttcaaa
    49201 atcttcaaaa gcaaaagttg cttgtctttc ctgttcttct agggcgcttc cagataattc
    49261 ttcctccgga tcgctgccaa taatctgctg acgaagaaag tcactcacat cttctgaatc
    49321 aacagtagaa tcttctgatt gagaaatttc ttcatccgga agatttttac tgataaacag
    49381 ttctaatccg tctccttttg gcaaaacttg gaaagtgaca gcttcgcttc ctcggaactc
    49441 ctcttcgata tcaacttctt ccaaaatact ataaaagaaa ttttcgattt ctttgtgatt
    49501 gccaagtaaa tccaaaaatg aaacgccccg ttcctctaaa tcttcatgac caattagtac
    49561 acggatcgta ttttcattaa tatgttccat ttccattttg ctacacctca ctttgccaca
    49621 atgtttaata tagattcatt gtagcgaaat cagcttaaat gtaaagaaaa aaaatctcgc
    49681 tgttaagcga atttaataaa accagcggat ttttcaacct ttcaagctat cttatttaac
    49741 tataacattt ttggcagacg aatactatat agccgacttt tcagctaatt ttccctctct
    49801 atttttcgtg caatcatcat cttctactac ctttttatac atctaatcaa gaatactttt
    49861 tgtaaaaaca aaaaaacttg gaaaaactat ctttgtaata gt
//
LOCUS       DAHUVV010000011        49682 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.011, whole genome shotgun sequence.
ACCESSION   DAHUVV010000011 DAHUVV010000000
VERSION     DAHUVV010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.011"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            257..532
                     /locus_tag="NK767_000616"
     CDS             257..532
                     /locus_tag="NK767_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693630.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2623
                     /locus_tag="NK767_000617"
     CDS             545..2623
                     /locus_tag="NK767_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCH6693631.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2783..4669
                     /locus_tag="NK767_000618"
     CDS             2783..4669
                     /locus_tag="NK767_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCH6693632.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4681..5628
                     /locus_tag="NK767_000619"
     CDS             4681..5628
                     /locus_tag="NK767_000619"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCH6693633.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5648..6175
                     /locus_tag="NK767_000620"
     CDS             5648..6175
                     /locus_tag="NK767_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCH6693634.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6238..6891)
                     /locus_tag="NK767_000621"
     CDS             complement(6238..6891)
                     /locus_tag="NK767_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCH6693635.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7023..7865
                     /locus_tag="NK767_000622"
     CDS             7023..7865
                     /locus_tag="NK767_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCH6693636.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8023..8919
                     /locus_tag="NK767_000623"
     CDS             8023..8919
                     /locus_tag="NK767_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCH6693637.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8922..9635
                     /locus_tag="NK767_000624"
     CDS             8922..9635
                     /locus_tag="NK767_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HCH6693638.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9655..10173
                     /gene="msrA"
                     /locus_tag="NK767_000625"
     CDS             9655..10173
                     /gene="msrA"
                     /locus_tag="NK767_000625"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCH6693639.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQDYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10170..10397
                     /locus_tag="NK767_000626"
     CDS             10170..10397
                     /locus_tag="NK767_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCH6693640.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10549..11100
                     /gene="lepB"
                     /locus_tag="NK767_000627"
     CDS             10549..11100
                     /gene="lepB"
                     /locus_tag="NK767_000627"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCH6693641.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11183..11683)
                     /locus_tag="NK767_000628"
     CDS             complement(11183..11683)
                     /locus_tag="NK767_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCH6693642.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11962..12360)
                     /locus_tag="NK767_000629"
     CDS             complement(11962..12360)
                     /locus_tag="NK767_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCH6693643.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12620..13480
                     /gene="ylqF"
                     /locus_tag="NK767_000630"
     CDS             12620..13480
                     /gene="ylqF"
                     /locus_tag="NK767_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCH6693644.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13473..14240
                     /locus_tag="NK767_000631"
     CDS             13473..14240
                     /locus_tag="NK767_000631"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCH6693645.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14296..15153
                     /gene="dprA"
                     /locus_tag="NK767_000632"
     CDS             14296..15153
                     /gene="dprA"
                     /locus_tag="NK767_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCH6693646.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15275..17353
                     /gene="topA"
                     /locus_tag="NK767_000633"
     CDS             15275..17353
                     /gene="topA"
                     /locus_tag="NK767_000633"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCH6693647.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17322..18698
                     /gene="trmFO"
                     /locus_tag="NK767_000634"
     CDS             17322..18698
                     /gene="trmFO"
                     /locus_tag="NK767_000634"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HCH6693648.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18896..19801
                     /gene="xerC"
                     /locus_tag="NK767_000635"
     CDS             18896..19801
                     /gene="xerC"
                     /locus_tag="NK767_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCH6693649.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19838..20386
                     /gene="hslV"
                     /locus_tag="NK767_000636"
     CDS             19838..20386
                     /gene="hslV"
                     /locus_tag="NK767_000636"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HCH6693650.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20400..21800
                     /gene="hslU"
                     /locus_tag="NK767_000637"
     CDS             20400..21800
                     /gene="hslU"
                     /locus_tag="NK767_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCH6693651.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21818..22609
                     /gene="codY"
                     /locus_tag="NK767_000638"
     CDS             21818..22609
                     /gene="codY"
                     /locus_tag="NK767_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCH6693652.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22691..23566
                     /locus_tag="NK767_000639"
     CDS             22691..23566
                     /locus_tag="NK767_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCH6693653.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(23698..24336)
                     /gene="plsY"
                     /locus_tag="NK767_000640"
     CDS             complement(23698..24336)
                     /gene="plsY"
                     /locus_tag="NK767_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCH6693654.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            24463..24897
                     /locus_tag="NK767_000641"
     CDS             24463..24897
                     /locus_tag="NK767_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCH6693655.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25063..27108
                     /gene="parE"
                     /locus_tag="NK767_000642"
     CDS             25063..27108
                     /gene="parE"
                     /locus_tag="NK767_000642"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCH6693656.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            27120..29570
                     /gene="parC"
                     /locus_tag="NK767_000643"
     CDS             27120..29570
                     /gene="parC"
                     /locus_tag="NK767_000643"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCH6693657.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            29847..32078
                     /gene="pflB"
                     /locus_tag="NK767_000644"
     CDS             29847..32078
                     /gene="pflB"
                     /locus_tag="NK767_000644"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCH6693658.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            32322..33083
                     /gene="pflA"
                     /locus_tag="NK767_000645"
     CDS             32322..33083
                     /gene="pflA"
                     /locus_tag="NK767_000645"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCH6693659.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            33168..34094
                     /locus_tag="NK767_000646"
     CDS             33168..34094
                     /locus_tag="NK767_000646"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCH6693660.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            34276..35169
                     /locus_tag="NK767_000647"
     CDS             34276..35169
                     /locus_tag="NK767_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HCH6693661.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            35303..35428
                     /locus_tag="NK767_000648"
     CDS             35303..35428
                     /locus_tag="NK767_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6693662.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(35562..36194)
                     /locus_tag="NK767_000649"
     CDS             complement(35562..36194)
                     /locus_tag="NK767_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693663.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(36342..36884)
                     /locus_tag="NK767_000650"
     CDS             complement(36342..36884)
                     /locus_tag="NK767_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCH6693664.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            37019..37408
                     /locus_tag="NK767_000651"
     CDS             37019..37408
                     /locus_tag="NK767_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693665.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            37412..38176
                     /locus_tag="NK767_000652"
     CDS             37412..38176
                     /locus_tag="NK767_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCH6693666.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            38305..39252
                     /locus_tag="NK767_000653"
     CDS             38305..39252
                     /locus_tag="NK767_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6693667.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            39382..40122
                     /locus_tag="NK767_000654"
     CDS             39382..40122
                     /locus_tag="NK767_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693668.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(40413..41081)
                     /locus_tag="NK767_000655"
     CDS             complement(40413..41081)
                     /locus_tag="NK767_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCH6693669.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            41215..41502
                     /locus_tag="NK767_000656"
     CDS             41215..41502
                     /locus_tag="NK767_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693670.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            41918..43456
                     /locus_tag="NK767_000657"
     CDS             41918..43456
                     /locus_tag="NK767_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCH6693671.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            43453..44418
                     /locus_tag="NK767_000658"
     CDS             43453..44418
                     /locus_tag="NK767_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6693672.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            44405..45727
                     /locus_tag="NK767_000659"
     CDS             44405..45727
                     /locus_tag="NK767_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCH6693673.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      45790..45894
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            45968..46711
                     /locus_tag="NK767_000660"
     CDS             45968..46711
                     /locus_tag="NK767_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6693674.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            46726..47718
                     /locus_tag="NK767_000661"
     CDS             46726..47718
                     /locus_tag="NK767_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCH6693675.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            47730..48467
                     /locus_tag="NK767_000662"
     CDS             47730..48467
                     /locus_tag="NK767_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6693676.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            48457..49179
                     /locus_tag="NK767_000663"
     CDS             48457..49179
                     /locus_tag="NK767_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCH6693677.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(49263..>49682)
                     /locus_tag="NK767_000664"
     CDS             complement(49263..>49682)
                     /locus_tag="NK767_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCH6693678.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        17145 a         8507 c        10410 g        13620 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaaacgag tccaataaaa taaaaaggta aaaattttca
     2641 agttttataa aaaacaaaac atatcagaac aggatcgaat cggtcctgtt ctttttttga
     2701 gaacacaaaa agaacaaata ccaaaaaaat tttcctgaag ttcactattt ttatgataca
     2761 atatccaaga taaggaggga acatgttgaa agaattaaaa gtaactgacc tcaaaaaaac
     2821 ttatggtgaa aaagatttgt ttgaccagat ttcttttttg gtccacgaaa aagaccgtat
     2881 cgggttgatt gggacgaacg ggacgggtaa aacaagtctc ttaaatatta ttgccggtat
     2941 cgatagcagc gacggtgatc ggcaaacagt gttttatcca actgattacc gtattggtta
     3001 tctttctcaa gcagctgaat tttccgatga attaactgtt ctccaagcag tctttcaagg
     3061 aaacagcccc ttgatccaaa ctgttcgagc atatgaggaa gcattgatcg aactagggga
     3121 aaacggagaa gaccctgatg tgcaaaaacg ttatgctaaa gcagaagaac aaatgaataa
     3181 agaagatgct tggactacag ataccaacgc aaagattatt ttgcaaaaat taggaatcag
     3241 tgaattagat aaaaaaatca gtactttatc aggtggtcag aaaaaacgag ttagtctggc
     3301 gcaagtgttg attgatgaac ccgatttact attactagac gaaccaacaa accatttaga
     3361 ttatgaagcg atcgagtggt tggaaaacta tttgaaacaa taccgtggcg cactattgat
     3421 ggttacccac gatcgttatt ttctagatag agtagcaaac cgaatcttcg aactttcatt
     3481 tggaaaatta tacgagtata aaggaaatta tgaagcttac gtcttagaaa aagcagaacg
     3541 tgatcgtgta gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac aggaattagc
     3601 ttggatgagg gctggcgtac aggcacgtgg caccaagcag caagcacgga tcaaccgttt
     3661 cgaagatcta aaggagaact tgtaccaagt aaaccaagaa gatgatttag agctgaacct
     3721 agcgacacaa cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt accgtataaa
     3781 tgaccaaaca ctgctagaac atttagattt attgattcaa tctcgggaaa gactaggtat
     3841 tactggtaaa aatggtgcgg gaaaatcgac attgcttaat attctagctg gcagaattcc
     3901 cctggatagc ggtacaatgt ctatcggtga aacggttcat cttgcgtatt atacacaaga
     3961 aaatgaagaa atggcaccag ataaacggat gattgcttac ttacaagaag ctgctgaaga
     4021 agcaaaaaca gcagatggca gtcaaatcgg agtagcagaa ttacttgaac gatttttatt
     4081 tccaagattt atgcatggga cactgatagg caaactttca ggcggagaaa aacgccggtt
     4141 atttttgttg aaactgctga ttcagcaacc taatgtttta ttattggatg aaccgaccaa
     4201 tgatttggac atagcaacat tgacgatttt ggaagattat tttagaagtt ttccaggtgc
     4261 tgtgatcact gtttcccatg accgttattt cttggacaag gtagcagata agttattagt
     4321 attccaagga aacggaaaac aagaacttta ttacggaaac atgtccagtt atttactgaa
     4381 gcaaaaagag acccagcagc ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc
     4441 tgctgaaaag aagaaattat cttacatgga acaaaaagaa tgggaaacga ttgaagatga
     4501 gattgcagaa cttgaagaaa aaatctcatt gctccaagaa gagatgaatc atcaaggaga
     4561 taatttcact cgattacaag aattgcaaaa cgacgtatct gaaacagaag cacaacttga
     4621 agaaaaaatg gcacggtggg aatacttgag tgaatgggta gaagattagg aggaaaaagg
     4681 atggaacaag cgtatcttga cttaggaaga aaattgttgg aagaagggca tcgtaaagaa
     4741 gacagaacag gcacaggcac attaagctta ttcggatacc aaatgcggtt tgatctgcaa
     4801 aaagggtttc ctttgttaac aacgaaacga gtaccttttg gattgatcaa aagcgaattg
     4861 ctttggtttt taaaaggaga tacgaatatc cgctatctat tacaaaataa aaaccatatt
     4921 tgggatgaat gggcatttga acgctacatc aaaagtccag actataatgg accagatatg
     4981 actgattttg gccatagatg tttgacagat cctgaattca atgaagaata taaaaagcag
     5041 agcaagcttt tttgcgagaa gatcctaacc gatgattcct ttgctgaaac atacggcgat
     5101 cttggacaca tctacggcta tcaatggcgt cactgggaaa caaaagacgg tggctttatc
     5161 gatcaaatca aacaagtgat cgaagcaatc aaaaagactc ctgattctag aagattgatc
     5221 gtttctgcat ggaatccgga agatgttcct agcatggctt tgcctccttg tcatacgatg
     5281 tttcaatttt atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat
     5341 gtgtttctag gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca
     5401 catgaaacag gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat
     5461 atcaatcata tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact
     5521 ttagttttga atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta
     5581 gaaggatatg acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga
     5641 tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga
     5701 ccgtcttcct tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa
     5761 tacactagtg atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg
     5821 tcagacgatt gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca
     5881 tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg
     5941 agggtcagct gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat
     6001 ccatcatgta ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact
     6061 gatcaatact agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac
     6121 gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag
     6181 ctatggatag actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta
     6241 agtaaagaat aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca
     6301 aacaacatgc atatagcgga tagaattcaa gctataaaag aatgcgccta tcgtgtaaga
     6361 aagcccgcct gctaccatca aaataacacc agtcattcca agagagtgat aaagaggaac
     6421 agcagccacg acacagagcc accccataaa aatatagatg atcgtagata cttttgatac
     6481 tctctcccgt ttatgcaacg tcaacgcttt atatataatg ccaagaatcg caaacaacca
     6541 gattaataca aataaagtcc aacctagcca tcctcggata ctcagcaaac aaaaaggagt
     6601 ataacttcct gcaatcaaaa gaaaaatcga actatgatcg aatacttgga acaccttttt
     6661 tgcttttgta aaaatcaatg aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat
     6721 cgatccatag atagcataag agacaatatg gagggaggaa tgcaagtggg caccttttac
     6781 taaaagaacc actagtcccg cgatactcaa tccagtccca attccatggg tgactgcatt
     6841 aaacacttca tttaatattt catacgttcg attaaatttc gttttttcca tgaaaacggc
     6901 tcctttacta ggtgtaatta atgaaaaatc attatttgtc tgttatacta aaaatagaaa
     6961 ttgtcttaca tgtagtttaa catgaataag gcatcttgtt aaagaaagag agagagaaaa
     7021 taatgacaaa tgtaaaaatc gtaacagatt cttcatgtac gatggaaaaa aatgtacgcg
     7081 atgctttagg cattcgtgtc atgccgctat ccatcatgat cgacggaaca gtatatcccg
     7141 atgatgacca actagacggc gaaagattta tggaaatgat ggcgaaagca aaaaatctgc
     7201 ccaaaacaag ccaaccacca attggagaat tcatctcgat gtatgatgag ctaggagaag
     7261 acggcagtga agtgatttcg atccatatga caaaaggctt gagtggaaca gtagaagcag
     7321 caagacaagc cagtaatcta acaaaaacga aagtaacggt tatcgacagt gataccactg
     7381 accaaggatt atcttttcag gtaattcgag cagctgaatt agcaaaagaa ggtaaaacag
     7441 tagagaaaat ccttgaagaa gtcaaaaaag tacaagacaa tacaaaatta tacatcggaa
     7501 tatccacact agataacttg gtaaaaggtg gaagaatcag ccgaacaact ggtttgctgt
     7561 ctagtgtttt gaacatacgc gtggtgatga atttcgccca ttctgaatta atccctgtaa
     7621 ctaaggggcg aggaaagaaa acgttcgata aatggtttaa taatttgaaa aatgaactga
     7681 aagaattgcc aaatgtgcga caaatcggta tttcacatgc ggatgcaaaa gaattggcgc
     7741 aagaattcaa agagggtttg caagaattat tcccggacat gcatatccct gtactgcata
     7801 cgaatccggt tatcgctacg catactggta aagacgcgtt tgcaatcatg tattatgtag
     7861 attagtcgaa aagaggataa gccctcagcc tgaaaggctt gcagcttatt ctctttttct
     7921 ttataaaatt aggaaaataa ttgttttctt ttttcataaa gactaccttt ttgataaaat
     7981 ttttgataac attagaacca atcgaataca agaggtgaaa aaatgaaatt aacgaaacga
     8041 catttgatcc tcgttttgtt cccattatta attgcgatca tcatctatgc aggtttagct
     8101 gcttctatac cgaaagctgg cccactaatg aaaccagtaa ctactgtgag agaagatcca
     8161 aataaaaaag aaaaacttca ctacatagct atcggagatt cgctgactga aggagtagga
     8221 gatcaaacca agcaaggagg attcgttccc cttgtcgcaa atgatctaaa agaccgttat
     8281 aatttgaccg cggttgaaac agaaaactat ggtgtaaatg gtgaacgaag tgaccagatc
     8341 ttaaagcgag taaaaacaaa agaagaaatt cgaaacaata tcaaaacagc tgattttatc
     8401 acactgactg ttggcggtaa tgatttgatg aaagttatcc aaaataattt attcgggtta
     8461 acggtcaatt cctttaaaaa gccactgaaa aactatcagg aaaatgtcac agaactattg
     8521 gaagaatttc gatccctcaa taaaaccgca cctatttaca ttttaggcat ctacaatcca
     8581 ttttatttga actttcctga gatcactgac atgcagacaa ttgttaacaa ttggaacaaa
     8641 ggaacagaaa cggtgacaaa aaaaatgccg aactgctact ttatcccaat caatgatttg
     8701 ctttatcaag gattgaatca ggaagtaggt gtcacagaag aaggaacaga tcaaacaact
     8761 agcacgacag aaaatctaaa tggcatcaaa aataatgtgc tctatgaaga agatcatttc
     8821 catccaaata atttaggtta tcaattgatg gcaaatgcag tgagagatca gctgatcgag
     8881 acacaaaacc aatggttaga gaaggaggat gatcaataaa catgaaaaca agatctgacg
     8941 tatcttcaaa caaaacaaag aaaacaaatt actggaaatt cgccttcctt atccttcttg
     9001 gggtagtact tggttcgacc gtatttttag gcagccgtat ttttgctaat cgggaaccag
     9061 aactcccaga aataccagct cttacggaaa gacaaggaga tcctgtctta acgatcaatt
     9121 caaataaaga aaaagtcaat caaatcatca gctttttcct tagtgagtat caaaaagata
     9181 gtgatattga atataaattt tatttggaaa atgaagcact actcaatggt actttcgaag
     9241 tactaggttt tcctatcgat ttctatttat attttgatcc gtatgtgatg gaaaacggaa
     9301 atgtccaact aaaagctaga agtctttcta tcggaacatt gagtttgcca atcaaagatg
     9361 tgatgaatat gatcaagaga aactataaat taccagattg gatcgaagtc aatacagatg
     9421 atctgactat catgcttagg ttggatcaat tccgaatgca aaacgggatg tatattaaag
     9481 cagataaaat cgacttagta aatgatgaca ttcggtttag tctatatttg ccaaaagata
     9541 cctctgatgc ttcaaacaac tcaaatacca acaaaactag caccagcagt actgaaagca
     9601 agacaaaaga aaataaaaaa tcatcggatg agtagtatta gagggggaat cataatggaa
     9661 cgagcaatat ttgcaggagg ctgcttctgg tgtatgatcc agcctttcga cacgcttcct
     9721 ggtatccata cagtgatgtc aggatatact ggagggcatg taccagatcc gacttatgaa
     9781 caagtaaaaa gccaaacgac tggacataca gaagcagtgg agattatcta tgatccaaca
     9841 ttgatttcat atgaacaatt agtagaactt tactggcagc aaactgatcc aactgacgct
     9901 ttcggacaat tcgaggatcg cggtgacaat tatcgcccgg tgatctttta tttgacagaa
     9961 gaacagcgga tcattgctga aaaaagtaag aaacagttag ctgaaagtgg acgtttcgaa
    10021 cagccaatcg ttacaaaaat cgaaccagcc caaccatttt atctagcaga agaagaccat
    10081 caagactatt ataaaaagca tcctgaaaat tttaaaagaa accatgcacg ccgggctgcc
    10141 tttatcgaag aaaattggag gaacgaaggg tgaatcgaag cttttttcat tatttaatga
    10201 cactgcgagg cggtggcaca catgatgctt tatctgtttt cgctacagaa gccggtaaag
    10261 acatccaatt tcctaagcac agctctagct acgaagaaat ttctaattat ctggaactga
    10321 atgtcgacta tttgccgtcc atggatatct ttgacgaagc atgggaaaaa tatttagaaa
    10381 ataatcatga catataagag atagttttgt actttatgat caaaaaagtt ttttgtccaa
    10441 tagcctaatt gaaagaatta tttttatttc agctatctag tcgcattttt ttgatatttt
    10501 tcttataata aagaaagaat gaaaaaaaga gggatggatt gatagagatt gacaaaaaaa
    10561 caacgctata ttaatcgctt ctggcttgta ttcaaatact tacttgtttc tgtattcctt
    10621 gcgttcatgt tgcggggatt tttattcatc ccggtaccag tggaaggcaa ttcaatggaa
    10681 aacgtgttga agcaaggtga tatggttgtg atggaaaaat tttcggagat cagacgattt
    10741 gatattgttg tttttcaatt agcagatggc acgatttata tcaaacgagt aatcggtctt
    10801 ccaggagaaa atgtcagcta ccaaaatgat caattgaaga tcaatgggaa agtagttaag
    10861 gaaccttatc taactaaaaa cctgaaatca gatcatgcaa atgcatccta tacgacggac
    10921 tttacactac aagagctaac gggacaatcc aagcttccgg aagataatta ttttgtactt
    10981 ggagataatc gaagagtttc aaaagatagt cgttcgtttg gcacgatcaa taaaactgat
    11041 attctaggta aagcaagatt tgtctattat ccacttgatg aaataaaatg gatccagtga
    11101 gaaacaataa agcgtgcagt ctgttgagac tgcacgcttt attgtttaat ccaaaatcag
    11161 tttactacct tagtacgtaa aatcaaacag ctcgtttaaa cacttttcta gcctctaaag
    11221 ttttgataaa tgaataaaca accattattt gaatagggat ttggatcata ttcctgatga
    11281 atctacctag aaaaattact ttgaaatctg caccaaacaa aacagacatc agccaagaat
    11341 tgataaaaag gctgactggg atcataatca ataattgggc agcgatgacg gaaggcaggt
    11401 ttatcttttt atgatataaa aataagccat taataaatcc accaaggaaa gccgtgataa
    11461 gaaaagcagc ttgaaacgtt cctgaaccaa atatcgtgaa atttaaagtg ttcgacaaca
    11521 tttcaagcaa accagcccag atcggtccat atagataacc cataagcgcc atacttaaaa
    11581 atccgaatgt gatttgaaaa taagcaggac caatcgctat cttgtataag atgacatcaa
    11641 gggctaataa aagagcagag gtcgacaaca atcttgcatt catttcgggc aatctaaaaa
    11701 cgtcttttgc aatcaaagtg ttcaaaatat tctctccttt gtcagttaat aataaaagga
    11761 taccataaaa tcaccaatgt gtcatgacac attggtgatt tacatacact ggcatctaac
    11821 aattaaaaaa agggtgagaa gactgctttt ataaactgta aaaaagagat tgtacgaata
    11881 aaaaaaatag aggcggagaa tacttatcgt atcatccgcc tttgtttttt tgttataaaa
    11941 aacgtcagat tagttatctg cttacatctt catccatacg ggttctaatg tatttccgtc
    12001 tagatcttgg acttctaatg aaaacatttc ttcttcaggc attcccatat ctacttcgtg
    12061 gaaagtacca ccattttctt ttgcaatttg agcaaatttt tttacttcat ccgcattttc
    12121 aacactaaac gaaatcaaag caccacttgt ttttttatta tcagctattt ctttatcttt
    12181 tataaatttt ttataaaaat catgagagag aagcattatc caaaaagtgt catcccatac
    12241 cattgaacca gcttcttcag tagaaaattc ttcatttttt ttaaaaccca gcttttcata
    12301 aaaagcaacg gaacgattga catcagcaac aggaaaatta acaaaaacca tactattcat
    12361 ttgtttcgtc tcctttctgt tttgttcgtc tatcatctct tatttaggaa acgctgtcaa
    12421 aaatttagat tcatatatag aaaccaaaat aaaaatttgc atcatcaaaa aagtgaagat
    12481 gtgatagaat gaggactggt tcactttcac ttaactaata gatggaattt ctttataata
    12541 aaaaagtgaa tttatattga aaaagaaaaa aacaagtaaa gccggggaat attccggtat
    12601 gaaaggaatg gagagaacaa tgaccatcca atggtttccg ggacatatgg caaaagcccg
    12661 acgagaagtt tctgaaaaaa tgaaatatgt ggacatcgta tttgaattgg tagacgcacg
    12721 attaccttta tcttctcgta atccaatgct agatcagatc attcagcaaa aacctcgcct
    12781 tgttttattg aataaagcgg atctggcaga tccacaacag acaaagctgt ggcaagaata
    12841 ttttcgtaaa caaggccatc ttgcattagc tatcacggca caagaaagta aaggaattaa
    12901 aggtctagta cctgcggcta aagaagcatt gaaagaaaaa cgggcaagag atgctgctcg
    12961 ggggatcaag cctcgcgcta ttcgggcaat ggtcttaggt attccaaatg ttgggaaatc
    13021 tacgttgatg aatcgactag taggcaagaa aattgcacag acaggaaata agcctggcgt
    13081 aacaaaaggt cagcagtggc ttcgtttagg taatgagttg gaattgttag acacaccagg
    13141 gattttatgg cccaaatttg aagatgaaga aatcggcaaa aaactagcgc tgacaggcgc
    13201 gatcaaagac cagcttgtcc atttagatga tttagcgatt tacggattag acttttttgc
    13261 ccgatattat tcaggaagaa tcagcgatcg ttatggttta tcaatcgagc aagaagaaaa
    13321 attggcaccg gaattactga tggatattac agcaaaacgt gggtataaag aagactatga
    13381 tcgtggcagc gaattagtcg tgcatgatat tcgtcaagga aaacttggac gatacacact
    13441 ggatcgttgc gaagaaatgg agcaaaaaag tgatgactga atccatccaa agtatcaaag
    13501 aacgattgaa aagcatacag agaatagatg atcctacact agaatcatta agaaatgatc
    13561 cacgcaaagg cgtgcagcaa gcactggctt cttttgaacg acgattgaaa aaggaagcat
    13621 taattaagga aaaatatgaa gaaatgtttt gctttgaaaa gcaagggtac gcacaaggtc
    13681 accaatggat tgccggcatt gatgaagtag gaagaggacc attagctgga cctgtcgtgg
    13741 cagctgcagt tatcttgccg aaaaatgaga tcatattagg gctgaatgac tccaaacaat
    13801 tatcagagaa aaagcgagaa cttctttatc aacaagttca agaaaaagcc ctggcaatcg
    13861 ggatcgggat cgttgatcag cagacaatcg atgatgtgaa tatttatgaa gcaagtaaat
    13921 tagcgatgat ccaagcagta gaaaaattgt ccattgtacc aagctatttg ttgatcgatg
    13981 cgatgacctt ggatttgcct attcagcagg aaaaaataat caaaggggat gcacgatctg
    14041 tgtcgattgc tgctgcaagt atcattgcta aagtatatcg agaccaattg atgaaagaat
    14101 atgacgtgct ttatcctgga tatggtttct cgcataatgc aggttatgga acaaaggaac
    14161 acttagaagg acttgaacga caaggaatta cgccaatcca tcgtaaaagt tttgcacctg
    14221 taaaaaaata tttacaataa aaaacagccg cttttacgta tcttattaga aagagagacg
    14281 aaaggcggtt ttatgatgta tcaaatagaa gaaaatttat tgaaacttgc ttatacaaaa
    14341 ggaatcagta gtcaaggaaa atgggcactt gctaactgga tgatgcagta tccttataaa
    14401 gaaatcggat ttgaagacat cattaagata ggtaaaataa ccaaacaccg ggagctgttt
    14461 cgcaaaagtt ggcaggaaat tcacgctaac tggggcaaaa tccaatcgaa acagtcgttt
    14521 atcacttgct ttgatcccaa atatccccct cagctgcttc acttgaccta tccgccaatt
    14581 gtactgtttt ataatggtga tctatcttta ctttcttgta acatgttaag cgttgttggt
    14641 ggaagacaca cttctgcttt agccaaaaaa acagtttatt atttactaaa accagtgata
    14701 gaagctggat atgtgatcgt cagtggctgc gcgaaaggga tagatacata cggtcaccaa
    14761 gcagcaatct ctcatggtgg acggacaatc gcagtcattg gaacagggat agagcaagtc
    14821 tatcctgctg aaaatcgtga cttgcaagag gtgattgcaa atgatcatct tcttttgagt
    14881 gaatatcctc ctcaatccgg cccgaaaaag cattgttttc caatgagaaa tcggattatc
    14941 gcagctttgt ctaaaggaac atgtgtcttt gaagccaaga aaaaaagcgg ctctctgata
    15001 acagcacagc aagcattgga tcttggaaga acagtttttt ctgttccagg aaatatttta
    15061 acagaacatt ctaccggatg tcatcatttg atccaagacg gtgcagtttg cacggtttcg
    15121 ggtgaagata tcttggcaga atttaaagaa taacaccata cttttcattt gcttttcttg
    15181 acaaacgttt tctgaatgga tatgattagc tacgatttgt atgaatcctc ttttaatagt
    15241 aggaacacga caatggaaag gagtccattc agtaatggcg tataaatatt tagtgattgt
    15301 agaatcacct gcaaaagcca agacaattga gaaatatctt ggacgaaact ataaagtaat
    15361 ggccagtgtt ggccatataa gagatttacc gaaaagtaaa atgggtatcg attttgaaaa
    15421 caattatgaa ccccattata tttctatacg cggaaaaggc gatgtcatca aaagcctgaa
    15481 agccgcagca aaaaaagctc aaaaagttta cttggcaagt gacccggata gagaaggaga
    15541 agcgattgct tggcatttag cgtacctact tgggttggat ctgaaagaaa aaaatcgggt
    15601 ggtcttcaat gaaatcacaa aagacgcagt caaagcagct tttaaggaac caagaacgat
    15661 cgatgtagat ttagtagatg cacagcaagc tcgtcgtacc ttagacagaa tcgttggtta
    15721 ttcgatcagt cctattctct ggcgtaaggt caagaaaggg ttaagtgcag gacgtgtcca
    15781 atctgtcgct ttaaaaatta ttattgaccg tgaaaaagag atccgagaat ttgttccaga
    15841 agaatattgg agcatcgacg gtaattttaa aaaagctcgc aagaaattca aagcaaattt
    15901 ctggggaatc gacggtaaga aaaagaaatt accagatgca caaagtgtaa aagaagtcac
    15961 tgctagaatt gatggaacag acttcgaagt taaaaaagta gaaaagaaag agcgcaaaag
    16021 aaaccctgcg aatcctttca ctacaagtag cctgcaacaa gaagctgccc gtaagttaaa
    16081 ctttcgaaca agaaaaacca tgatggtagc acagcaatta tatgaaggaa tcactttggg
    16141 taaacaagga acagtcggct tgattacata catgcgtact gattctaccc ggattgccga
    16201 tacagcaaaa gcagaggccg cagcatttat tgaagatacg tatggaaaag aattttctag
    16261 acatgatcac cgaaagatca aaaatgctca aggcgcacaa gatgcccatg aagctgtgcg
    16321 tccaacgagt gtgcttcgta caccagatga actcaaacaa tatttagata aagatcaatt
    16381 gaaactttat acattgatct ggtcacgttt tgtggctagt cagatgacac cagctatttt
    16441 agatacaatg aaagtgacat tagaacaaaa tggtgtcatc ttcattgcga acggttcaaa
    16501 aatcaaattc aaaggattca tgcaagtgta tgtggaaggt agagatgacg gcaaagaaga
    16561 gaaagaaaat attcttccag aacttgaaga aggcgatgta gtccaatctg ttgatattga
    16621 accaaaacaa cattttactc aaccaccagc acgttttagt gaggctacgt tgattcgtgc
    16681 actagaagaa aacggggtag ggcgaccttc cacttatgca ccaacacttg atacgatcca
    16741 aagacgctac tatgtaaaat tgacacaaaa aagatttgag ccgactgaac taggagaaat
    16801 tgtcaattca ttaatctgtg aattcttccc tcaaatcgtc gatatccatt tcacagcaga
    16861 aatggaagga gatttggata aaattgaaga aggcacggaa gcgtgggtca aagtggttga
    16921 tcgtttctat aaaccgtttg aaaaagaact gacgaatgcg gaagaaaaaa tcgaaaaaat
    16981 tcaaatcaaa gatgaaccag caggattcga ttgtgatgtc tgtggacatc cgatggtgat
    17041 aaaattaggg aaatatggga agttttatgc ttgcagtaat tttcctgatt gtcggaatac
    17101 gaaaccaatc gtaaaagaaa tcggtgtgac ttgcccagta tgtcatgaag gacaagtcat
    17161 tgaacgaaaa tctaagaaaa acagactatt ctatggttgt tcccgtttcc cagaatgtga
    17221 attcacttct tgggataaac cagtgggcag aaattgtcca aaatgtgacg gatatctggt
    17281 cgagaagaaa gtcaaaggcg gtaaacaagt agtctgtgta aatggagatt acgaagaaga
    17341 catccaaaaa taatagaaaa gaggttcttt cattgaaaat ggtaaatgtt gttggagcag
    17401 ggcttgccgg aagtgaagct gcttggcaga tcgcacaagc aggagtccct gtccgtttat
    17461 atgaaatgcg tccagtcaaa tcgactgagg cgcatcacac aagtaatttt gctgaactag
    17521 tatgttccaa ttcattaaga ggaaacagtt tggctaatgc agtagggtta ttgaaagaag
    17581 aaatgcgccg attgaattcg gtagtaatca cttcggcaga tgaaacagca gtgccagctg
    17641 gtggtgcctt agctgttgac cgcgattctt tttctgaaac aattacccga aaagtcaaag
    17701 agcatccatt agtaacagtg ataaacgaag aaattactga aataccggaa ggaatcaccg
    17761 tagtagcaac aggacctttg acttctcatc cattagcaga aagcatcaaa gcattcaatg
    17821 gttcagatgg tttttatttc tatgatgcag ctgcaccaat cgtagacaaa gctacgatcg
    17881 atatggataa agtatatttg aagtcccgct atgacaaagg agaagcagct tacttgaact
    17941 gtccgatgac agaagaggaa ttcaaagctt tccatgaggc actaatcaat gccgaagtgg
    18001 cagaacttaa atcttttgag aaagaaaaat attttgaagg atgtatgcca attgaagtaa
    18061 tggcacagcg aggattcaaa acgatgcttt tcggacctat gaaaccagtt ggattggaag
    18121 atcctaaaac tggcaaacgt ccttatgctg ttatccagct gcgtcaagac aatgctgcag
    18181 cttctttata caacatcgtg ggctttcaga ctcacttgaa atggggcgag caaaaacgtg
    18241 ttttccggat gattcctggg ttagaaaatg ctgaattcgt tcgctatggt gtgatgcacc
    18301 gaaacagctt tatgaattcg ccagaattgc tgaaaccgac ttaccaatca acgaaacgcg
    18361 aagatctgtt ctttgcaggt cagatgactg gtgtagaagg ctatgttgaa agtgcagcga
    18421 gcggactact tgctggaatc aacgccgcac gcttagcaaa agaaatggaa ccagtcgagt
    18481 ttccacaaga aacagctatt ggaagtatgg cttactacat tactcatgcg gaaggaaaac
    18541 atttccaacc gatgaatgcc aactatggtc tgtttcctga actgccagag cgcattcgag
    18601 acaaaaaagc aagatatgaa gcaattgcac aacgagcttt aaaagctaat gaggcaataa
    18661 aaacagaatt agaaaaaact gcaatcccaa atgagtaagg aaactccatg aagagggtga
    18721 gacggatagt ctcgctcttt ttcttgcgtt tttcttaaga atttctcaaa caaaatgtga
    18781 ggaattgcgc caattgtgaa caaacttaga aatattggtt aaattctgac aatttatttg
    18841 tctttttata aacgctgtgc taaattattc tctgggacaa aaggaaggtg agaaaatgac
    18901 agaagccacg tgggaacaaa aatttttgcg ttatttgatt gtcgaaagag gatattcaga
    18961 aaaaacgaaa gaagcatatg aagaagacat tctccatttc aaacattttt tggaggaatc
    19021 tggagacgct gatctgctga aggcggaata ttttacggtt cgtgtctatt tgagtgcatt
    19081 agctgatcat cgtcctagct atagcagaaa ttctatcagt cgcaagatag ccagcctacg
    19141 ttcgttttat cagtatcttc tcaaagaaga agtgataaaa gaaaatccat tttcttacgt
    19201 tcatctcaaa aagaaaaatt tgagactgcc acgttttttt tatgaaaatg aaatgcaagc
    19261 actttttgac agcgtagcag gagatagtcc tttggatttg aggaaccgcg ctttactgga
    19321 agtcctttac gggtcaggca tccgtttaag tgagtgtagc agtttgtcgg tatcagacat
    19381 tgattttgat tcagaggtca tgttgatcca tggtaaaggg aacaaagaga gatatgctcc
    19441 tctaggttct tttgctcaag atgctctgca agaatatttc agggaaggcc gccaggtatt
    19501 gatgacaaaa tatcacgaag agcatgatat cgtttttgtg aatcatcatg gaaaaccaat
    19561 cacgcctact ggaattgaat atgtcttgaa tcaaatgatc aaaaaaagca gtttggatag
    19621 tgatatccat cctcacatgc tgcgccatac atttgctact catttattaa ataatggggc
    19681 agatttgcgg acagtccaag aattgctagg acatgccaat ttatcgacta cccagatcta
    19741 tgcccatgta acaaaagaaa gtttacaaaa aaactatcgt tctttccatc caagagcgta
    19801 aaaataagga aaagaacaaa tttaggagga aacaaacatg gtcgaatcac catttcattc
    19861 aaccactatc tgtgccgtcg aaaaagacgg gaaatttgct atggctggag atggccaagt
    19921 aacaatgggt gaatctgttg tcatgaaagg cactgcaaag aaagtacgac gtatctacaa
    19981 tggagaagtt gttgtaggtt tcgccggaag tgttgcagat gcgtttactt tagaagaaaa
    20041 atttgaaggc aaactaaacg aatataacgg gaatctacaa cgcgcagctg tcgaattagc
    20101 tcaagaatgg cggacacagc aatctatgca aaaacttgaa gcgatgctga tcgtaatgaa
    20161 tgataaagaa atgcttcttg tttcaggtac aggagaagtt atcacaccag atgatggtat
    20221 cttagctatc ggatcaggag ggaattttgc gttggctgca gcccgagcaa tgaaaaacta
    20281 tgctcaaaac gacatgagtg cgaaagaaat tgcagaaaat gcgttaaata ttgccgcaga
    20341 tatctgtatc tttactaatc ataatattat cgtagaagaa ctgtaagagg tgagtagata
    20401 tgtatgaatt aaaacaaaca ccaagacaaa tcgtaagtga attagatgaa tatatcgtag
    20461 gacagcacgc agcaaaaaaa tctgttgccg ttgctttgcg taaccgctat cgccgtttgc
    20521 agttagatga aaaaatgcaa caagatatta caccaaaaaa tatgctgatg atcggaccta
    20581 caggggtcgg taaaacagaa atcgcacgac gtttggcaaa aatcgtcaat gcaccatttg
    20641 tcaaagtcga agcaacaaaa tttactgaag tcggttatgt cggaagagat gtagaatcaa
    20701 tggttcggga tttagtagaa aatgcgatcc aaatcgtaga aaaagagcag tacagccgtg
    20761 tatacacaag tgctaaaaaa aatgcagaaa aacgtttggt aaaaatcttg gtacctggta
    20821 tcaaaaaaga acagaaacaa tccactaatc aatttgaaca aatgatgaat ctttttaatg
    20881 ctagtcagca aaataccgct gaaccgcaag aagaagtgac agaagatatt cgaatcaatc
    20941 gccgtacagt cttggaacaa ctacaaaagg gtttactgga tagtcgtgaa gtaaccattg
    21001 aagttgaaga ccaaaagaaa cctgcaccaa tgatgaacaa tggattggag caaatgggga
    21061 tcgacctaaa tgaaactcta ggtgctttga caccgaagaa aaaagtggaa cggacggtaa
    21121 cagtcaaaga agctttagag ttattgatca aggaagaatc agcaaaatta gtcaaggatg
    21181 ctgatatcca tagtgaggca atcaaattag cggaagaaag tggaattatt tttatcgatg
    21241 aaatcgataa gatcacttct aaatcacagc aatctggaga agtatccaga gaaggcgtcc
    21301 aaagagacat attgccaatt gtcgaaggtt cacaagtaaa cacaaaatac gggatcatcc
    21361 aaacagatca cgtcctattc attgcttcag gagctttcca tttgtctaaa ccaagtgatc
    21421 taattccaga acttcaagga cgcttcccga tacgggtaga attagatgat ttgacagctg
    21481 aagattttgt gaagattctg accgaaccaa ataatgcttt agtaaaacaa tacgtcgcgt
    21541 tgatcggtac agaaaatgtg tcagttacgt ttacaaaaga agcaattgaa cggatcgctc
    21601 aaatcgcttt cgatgtcaac cgtgacacag ataatatcgg cgcacgcaga ctgcatacca
    21661 ttttggaaaa actactagaa gatctattat ttgaagcacc agatatgcaa atgggagaaa
    21721 taacgatcac agaagcatat gtggatgaca aacttggctc aattgcggca aatgaagatt
    21781 taagtcgcta tattttataa aaaatcggag gacgacaatg acagacttgc taacgaaaac
    21841 ccgcaagata aacacacttt tgcagcaaaa gaatactttt gacatgcaag cagatttgcc
    21901 ttacgataaa atggctgtca cactaggtgg tattcttgac agcaacacct atatcatagg
    21961 aagtgacgga gtattactag gttataatga acgtcacgat gtgaacaatg aacgaatcaa
    22021 aacaatgttt gttgagaaac aatttcctga atcttatact gatacagtgg cacagttgat
    22081 gaaaactgaa gctaatatcc caattactag tgatattact gcatttccgg tagagtcaag
    22141 agagaaatat ccatttggtc tgacgactgt cgttccgatc tttggagctg gcgaacgttt
    22201 gggtacgatt atcttatctc gtatggaaca atcgtttgca gatgacgatc tagtcttagc
    22261 agaatatgga gctacggttg tcggtatgca gatcctttat cagaagtctc gaagcatcga
    22321 agcagatgta cggagtgcaa cggctgtcca aatggcaatc aacacattat cttatagcga
    22381 attgaaggct gtacaagcta tctttgaagc attggatgga gatgaaggtc gtctgactgc
    22441 ttctaatatt gccgatagta tcggcatcac tcgttctgtg atcgtcaatg ctttacgaaa
    22501 attggaatca gctggaatca ttgaatctcg ttcgttaggc atgaagggaa cttatttaaa
    22561 agtattgaat tcccgtttta aagacgagtt agcaaaacac agttattaga agtaagggga
    22621 ctggggcaga tagaaatgtt tgttccagcc ttcccatttt aaaaagtaag tcgaagttgg
    22681 aggaatggca atgactgtta ttattcaaaa tgaccagctg atcgccgaaa tcgctgaaca
    22741 cggtgcggaa ttgatcagtc tgaaatcaaa agaacatggt ttagagtata tctggcaagg
    22801 tgatccagcg tattggggca gacatgcacc ggttttattc ccttttgttg gacgtttaaa
    22861 agatgatcag tatgtttatc aagggaaaac atatcaaatg gggcaacacg gatttgctcg
    22921 ggatatggat tttgaagtca ttgaacatgg acaagaaatg gctagtttca tcttaaggag
    22981 tacgccggag acattagaaa agtatccatt tgattttgaa ttagtgatca gctatgaatt
    23041 aggaggagac ggcatcacgg ttcattatca agtggcaaat accggtgaaa aagaaatgtt
    23101 cttttctatc ggtggacatc ctgctttcaa tgtacctctt gaagaaggat tgtcatttga
    23161 agatttctat ctggcatttt ctccattgaa atcaagatta cgcctaccat tgaaaggccc
    23221 attcatcgat atggatcaaa agacgttagg tcagacaaat acgaatctgg ctttgaatcg
    23281 tgaacttttt gagcaagatg ccttggtttt tgaaacaagg ggtctaaatg cgtacgccat
    23341 tcgaagtgaa aaatcttccc atagcgtaac tttaagctat aaagatatgc cttatgtagg
    23401 gatctggtct ccttatccaa aagaagcgcc tttcgtttgt atcgaaccgt ggtgcggaat
    23461 cgctgacacg gtgaacagta gtggaaactt gatggaaaaa gtagggatca atcaactagg
    23521 tgcccatgaa atcttcaaga caaaatattc catcacagtg aagtaagagg ttgtgaccga
    23581 gaagttagca gggaacaccg tttaccaagg tgtgtaaaaa agctatcctg taagtaataa
    23641 gggagtgtga catcgatttg tcacactccc gttttatctc tttggaatga aaaagtatca
    23701 ggattttttc ttgctgctat tcaaaccaaa cgggattcga ctttcagtat ggtttctgat
    23761 acgcttgata ttgtctttat gccgataaaa gataaaggaa gaaactaaaa aggcaatgat
    23821 cgtcagtaac caattgggct tagccaaaat cacaggagct acataaggaa ggacgatcgt
    23881 agataaagtg atcaatactg cactgatcat actgctcaaa ctcaccatac ttgtacaaaa
    23941 taaactaacg ataaagatga tacaagaata aacaaaaaat acaggattaa atcctaaaag
    24001 cattcctgca cttgtcgcaa cagcttttcc tcctttgaac ccagcaaaaa tcggaaatgt
    24061 atggccaaga atggcgcata ccccaaacca aagagggtta atcccagaaa ggtggaagat
    24121 catcggcaaa caagtagcca atgtaccttt taaaatatcc ataagcagga caacaatccc
    24181 ggctgttttg cctaatacac gaaatgtatt ggtcgttcca gtattaccgc tcccgtgttg
    24241 tcggatatct tttttgaaaa acaatttgcc aatccagaca ccggaaggaa tagaacctaa
    24301 aagatatgct aaaataagta ataagatcaa tttcattttt taccctcttt caagttgaaa
    24361 actatccttt attttagcat gaataggtaa caggaacaat tgaaaaaatg aaagatacaa
    24421 gtataataga tggaataaaa agaaaagcaa ggggtggcag aaatgtctgt aaaaaatcca
    24481 acacaagaaa cgattttcaa ctatttggaa gaagcaaaaa atattgccgt cgtaggcttg
    24541 agcaataaac cagaacgaac aagttacatg gttgcacagg tattgcaaga caatggatat
    24601 aaaatcatac cagtaaatcc attattggct ggtgaagaaa ttctaggcga gaaagtctat
    24661 gcgaatttga cagaaatcga tgatccaatc gatatcgtag atgtatttag acggagtgaa
    24721 tttttacctg aaatagcagg tgattttttg aaaactgatg ctaaagtgtt ctgggcacaa
    24781 ttagggattg aaaacaaaga agcagctgag atgctaagaa aagcaggacg tacagatatc
    24841 gtaatggatc gttgtattaa gattgaactt ggtaagttga aagaaaacca aaaataaaaa
    24901 tgaccacaaa gaagagatga tcgatagagg ggaatgtcga aaaagcggtt aggagataat
    24961 taaaattgat ttctcgtcta ccctcttttt tttcacgacc ttttctagta tgataatcgt
    25021 ggtgcggttt tgccgcaaag tattattgaa ggagcgtttg ccttggcaaa aaaagtaaac
    25081 aacgaataca atgactcgtc catccaagta ttggaaggat tagaggcagt tagaaaaaga
    25141 ccagggatgt acattggatc cactgacagc cgaggcttgc atcatttagt ctatgaaatc
    25201 gtcgataatg cagttgacga agcattatca ggttatggca gtgagattga tgtgaccatc
    25261 catgaagaca atagtataac tgttgcagat agcggacgtg ggatgcctgt aggaatgcat
    25321 gcttccggaa taccaacagt tgaagtgatt tttactgtgc tgcatgctgg cggaaagttt
    25381 ggtcaaggag ggtacaaaac ttctggtgga ctgcacggtg taggagccag tgtggtaaat
    25441 gccttatcga aatggctaat tgtaacgatc gttagagatg gcgtagagta tcagcagaaa
    25501 tttaaaaacg gcggtaaacc tgacggtact ttgaaaaaga taggaaaaac aaaaaaagca
    25561 aacgggacga ctgtccattt tttaccagat gatacaatat tcagtacaac gaaattttca
    25621 tatgaaattt tagccgaacg gttaagagaa tctgcctttc ttctaaaagg cgttaaaatc
    25681 tctctttctg atcttcgtgg agaagaacca gtcaaggaaa tttttcatta cgaagaagga
    25741 atcaaagaat ttgttgatta tctgaatgaa gaaaaagata cattgacacc agttgtttat
    25801 ttttcaggag aaaaagaagg tatcgaagta gaggtcgctt atcaatacaa tgacggttat
    25861 tcagaaaatg ttttatcttt tgtcaataac gttcggacaa aagatggggg aacccatgaa
    25921 gctggtatga aagcggcgat gacgaaatct tataatgaat atgcgagaaa agttggtttg
    25981 ctgaaagaac gggacaagaa tctggaaggt agtgatttca gagaagggtt agctgctgta
    26041 ctttctatcc gtgtacctga aaatcttctg caatttgaag ggcaaacaaa agaaaaactt
    26101 ggtacgccag ttgctcgtac cgtagtagat aacgtcatca gtgaacaaat gggcttttat
    26161 ttgcaggaaa atagcgaaat gagccagatg ttggtccgta aagcaatcaa agccagagaa
    26221 gcgagagaag cagcacgtaa agccagagaa gaaagccgaa atgggaagaa gcgtaaaaaa
    26281 ggcgaatctc ttctttcagg aaaattgaca cccgcacaat cccgaaaccc taagaaaaat
    26341 gaattgtatt tagtcgaagg ggattctgct ggcggatctg ctaaacaagg aagagacaga
    26401 aagttccaag caattctacc tttacgagga aaagtcatca ataccgaaaa agctaaaatg
    26461 caagatatct tgaaaaatga agaaatcaat acgatgatct atacgattgg tgcaggagtc
    26521 ggtcctgagt tttctatcga agactgtaat tacgacaaag taatcatcat gaccgatgcg
    26581 gatacagacg gcgcccatat tcaagtatta ctgcttactt tcttttatcg ttatatgaaa
    26641 ccgctgattg aagctgggaa agtatatatc gcgttaccgc ctttatataa agtatccaaa
    26701 ggacaaggaa agaagcaagt cattgaatat gcgtggacag atgacgaatt ggcagcaatg
    26761 attaaaaaag tcggcaaagg atatatgctg cagcgatata aaggacttgg agagatgaat
    26821 gcggaacaat tgtgggaaac aacgatggat ccgacttccc gtacgttgat ccgcgtccgt
    26881 atcgatgatg ctgcgcaagc tgaacgtcga gtaaccacac tgatgggtga caaagtagaa
    26941 cctcgaagaa aatggatcga aaatcacgtg caatttacgc tcgaagaaga tggcagtatc
    27001 ttagataaaa aagaggatac tgaaatctct ccttcagttt ctaatgattt attagatgaa
    27061 gaacgagcag ataaaaatga aaacaatcag ttatttgaag tagaatagga ggtcacctga
    27121 tggaaaatcg acaagaagta caagaattaa ctttagaaga agtgatgggt gaccgcttcg
    27181 gacgttattc caaatatatt atccaagaac gtgcactgcc tgatatccgt gatggattga
    27241 agccagtgca acgccggata ttatttgcca tgaacaaaga tggcaatacg tatgacaaag
    27301 gattccgtaa atcagcaaaa tctgtcggaa atatcatggg gaattatcac cctcatggtg
    27361 accgcagtat ttatgaagcg atggtccgca tgagccaaga ctggaagttg cgggaagtac
    27421 tgatcgaaat gcacgggaat aacgggagta tggacggtga tccacctgct gcgatgcgtt
    27481 atactgaagc cagattatcc aagttaagtg gagagatgct agcagatatc gagaaagaaa
    27541 cagtcgatct cgtgtggaat tttgatgata cagaaaaaga accaacagtc cttccagctc
    27601 gatacccaaa ccttctcgtc aatggatcga caggtatatc tgctggatat gcaacagaaa
    27661 tcccgactca taacttagct gaagtgatcg atggcacaat ctatatgatc gatcatccac
    27721 aagcgagcct tgaaaaattg atggaatata ttcctggacc tgactttcca acaggaggga
    27781 tcttgcaagg aaaagatgaa atcaaaaaag cttatgaaac aggacgagga aaagttattc
    27841 ttcgttcgaa aacaaagata gaggcaatga agggtaataa acagcaaatc gtgatcacag
    27901 aaatcccata tgaagtaaat aaagcgacct tggtgaaaaa aatggatgag atccgtttga
    27961 ataagaaaat cgatggaata gctgaagtac gagatgaaag tgatagaaca ggtctgcaaa
    28021 ttgtcgtcga attgaaaaaa gacgccaatg ctcaaggaat tttgaattat ttgttcaaaa
    28081 acactgagct acagatcaat tacaatttca atatggtcgc tatcgaccat atgactccgc
    28141 atcaagttgg actgaaagac attttaagaa gttatatcga gcatcgaaag caagtcataa
    28201 caaaacgtag tcaattcgat ttagccaaag ctcaaaaaag acagcatatc gtagaaggat
    28261 tgatgaaagc tttatcgatt ctggatgaag taatcgcaac gattcgtgaa agtaaagaca
    28321 aaaaagacgc caaaaagaat ttagtcgacg tctttcaatt tacagaagaa caagcagaag
    28381 caattgtcac attgcaatta taccgtttga cgaacacgga tatcacagag cttcaaaagg
    28441 aatccgaaag cttgattgcg caaatcacag agttgaataa aatcctttca aatgacaaag
    28501 agcttttctc tgttatgaaa aaagaattgc gagaagtgaa aaaaaattat tcttccgctc
    28561 gtctaacgac aatcgaagac gaaatcgaag aaatcaaaat tgatacgcaa gtcttagtgg
    28621 cacaagaaga cgtaattgtc agtgtgacgc gagaaggcta tgtgaagcga acaagccttc
    28681 gttcttatag tgcttccaaa ccagaagaaa tcgggatgcg agaaggcgac tacttgctat
    28741 actcaggtga attgagtaca ttggaccatg ttcttctgat cacaaataaa gcaaatgtga
    28801 tctaccgccc tgtccatgaa ctacccgatt tgaaatggaa agatgctggt gagcacattt
    28861 ctcaaacaat ctccaatctg tctgtagacg aatcgatttt agctgttttc ccatatcaaa
    28921 aaattcaagc agagaaaaca tttgtattca tcagcaagaa tggattgatc aagcaaacac
    28981 gaatgacaga gttcgaacca tggcgcacct ataagagccg tccgctcagc tgtatgaagc
    29041 taaaggcgga agaagatgaa ctgattgctg tgtatctgaa agaagatcag ccaggcttag
    29101 atgttttctt agtaacgcat caaggaatgg gtttgcgcta tcctttaaca gatgttcccg
    29161 ttgttggagc aaaggcagct ggagtgaagt ctatgaattt gaaagatgga gataggatta
    29221 tcaacgggct gcttgtgtta gcagaaggtg atacgccgat cgtaatcgtt actcagcgag
    29281 gagctgttaa acgcatgctt gctcaagaaa tcagccaaac ttcacgtgcg aagcggggag
    29341 tgacggtcat gcgtgagctg aaaaaaaatc cgcaccgtat tatctatatg tcggaaggaa
    29401 gatcaaaaga aataaccttg atgaatcaaa aagggcagca gttaacgatt gacccaactg
    29461 atttccctat aggtgatcga acatcgaatg gatcatttgc gatggatgag aaaaaaggtg
    29521 gagaagtcat cgaagttatc gatgctcctg agattttcat cgagtcgtaa ctgttgtagt
    29581 attttaatga agagacgcca tctgtattaa tacaggtggc gtctcttttt cattcatttt
    29641 taataaatcc ttatacgttt gcaaaaaaag atgaaagtgt tcacaatgct taaatgttgg
    29701 tatgtcaacg ttgatttaaa tatatgtaaa taaaacataa aaattgttaa ataaaaaaca
    29761 agaaaaccct tgcaaagata gggtgctgtg ttatactaat cacgtaaaaa ctgttacata
    29821 aaacagaaat ttaggaggtc catacaatgg agcaatggaa tggatttaaa ggtaccaaat
    29881 ggaaagaagg cgtagacact agagacttta ttcaaaataa ctacactgaa tatcgcggtg
    29941 atgacagctt tttggaaggc ccaacagaag caacagaaaa attatgggat gaattgcaaa
    30001 agttaaatga tatccaattc gagcgtaacg gtgtctatga tatggacaca gaaatcgtat
    30061 caactattac ttcacacgat gcaggctatt tggataaaga tttggaaaaa atcgtcggtc
    30121 tacagacaga caaacctttg aaacaagcat tcatgccgtt cggcggaatc aatatggcta
    30181 acaatgcgct aaaaagcaat ggctatacac caaacgatga attgacacat attttcactg
    30241 actggcgcaa aacacataat caaggtgtat ttgacgctta tactgctgaa atgcgtgctg
    30301 ctcgtaaaaa caaaatcatt acagggcttc cagacgctta cggtcgcgga cgtatcatcg
    30361 gtgactatcg tcgtgtggca ttgtatggaa tcgactattt gatggaacaa aagaaaaaag
    30421 atcatgatat gactggttat catacaatga gtgaagaagt gattcgttta cgtgaagaaa
    30481 tcactgaaca atatcgtgca ttagcgcaaa tgaaagaaat ggctgcgaaa tatggctatg
    30541 atatttctaa accagctgca aatgcaaaag aagctgtcca atggctatac ttcggatatt
    30601 tagcagcaat caaatcacaa aatggtgctg cgatgtcaat cggacgtatc tcagcattcc
    30661 ttgatatcta tatccaacgt gatttggata atggagtaat cacagaaaaa gaagcacaag
    30721 aattgatcga ccacttagta atgaaattac gtatggtcaa attcgcgcgt acacctgaat
    30781 ataaccaatt attctcagga ttccctatct gggctacttt atccattgca ggtatgggat
    30841 tagatggtcg ttcattagtt acaaagaatg atttccgtct gcttcataca ttaacaaata
    30901 tgggaccttc accagaacca aacttgactg ttttgtattc tgaacacctt ccagaaggat
    30961 tcagaacata tgctagcaga atcgctatcg aaagttcttc gattcaattc gaaaatgata
    31021 atcagcttcg tgaacaatgg ggttctgatg attgtgcgat tgcttgctgt gtatcaggaa
    31081 cagttgtcgg aaaagacatg caattctttg gcgctcgtgc taacttggct aaaacactat
    31141 tatatgccat caatggcgga gtcgatgaag tgactggcgc ccaagttgga ccagaatatc
    31201 gtccaatcac atcagaaata ctagactttg aagaagtgaa acataacttc atgaatatga
    31261 tggattggtt agcagaacta tatgtcaata cgttgaacat cattcattat atgcatgata
    31321 aatattctta tgaagcagct cagctggctt tgatggattc tgacttgcaa cgcacatttg
    31381 ctacaggtat tgctgggatc tctcatgcgg cagacagctt gtcagcgatc aaatatgcaa
    31441 aagtaaaagc aatccgtaat gaaaaaggaa tagcagttga ttttgaagtg gatggcgact
    31501 tccctaaata cggaaatgat gatgaccgtg cagatgaatt agtttcatgg atcttggaat
    31561 acttcatggg acaaatcaaa cgtcataaaa cttaccgcaa ctcaaaacca acaacttcat
    31621 tattgacgat tacttcaaat gttgtttacg gtaaaaatac aggaaatact cctgatggcc
    31681 gtgaagctgg aaaaccactt gcgccaggag caaatccaag ttacaatgcg gaacaaagtg
    31741 ggttattagc tagcctgaac tcaacagcta aaatgaatta cggctgtgca ttggatggaa
    31801 tttcaaatac ccaaacaatc aatccttcag gtcttggaaa agaacaagat acaagaatcg
    31861 aaaacttgcg taatgtctta gatggatact tctcaaaagg agcttatcat ttgaatgtca
    31921 atgtattcag taatgattta ttgcgtgatg caatggaaaa tccaatgaaa tatccgaact
    31981 tgacgatccg ggtatctggt tatgcagtta aattccgtga cttgacaaga gaacaacaat
    32041 tagatgtctt gatgagaact tcacacgaca gaatgtaaaa agttcgtgaa aaaagttttt
    32101 cgacttagcg tcgaaaggac gttgaatagc catcgcatca aaatcgaata atcagtatgc
    32161 ttatttgaaa gaaacgattt acaggggcag gacttgtatg ttccgtccct gtaaatttta
    32221 cacataacga agcggcaatt gcgaaaaatt ttgttcagaa agcttcgctt cgacagctaa
    32281 cttgtactta aaatgaaatc aaagaaaaga tgtgaactta aatggaagaa aaaacaatcg
    32341 gctatgttca ttctatcgaa actttcggtt cagtagacgg acctggactg cgctttgtcg
    32401 tgtttatgca aggatgccgc atgcgttgcc aattttgtca caatccagat acttggaata
    32461 ttggcggagg gaaagaatac acagcagatg aacttttaga taaagcagaa cgcttcagac
    32521 cttactgggg agataaaggt ggtattactg ttagtggtgg agaacctcta ttgcagatcg
    32581 attttttgat cgaactattt aaaaaagcaa aagagcggaa gatgcatact acattggata
    32641 catgcggaaa accttttacg tatgaagaac ctttcttttc acgttttcaa gaattgatga
    32701 agtatacaga tttactgtta tttgacatca aacacatcga taatgaagag cataaaaaac
    32761 taacgcattg ggacaatgaa aatattttag aaatggctca atatttgtca aaaatcaaca
    32821 aaccggtttg gattcgccat gtactagttc ctgagcgaag tgattatgat gaatatttga
    32881 tccgactaga caactttatt caaaccttgt caaatgtaga cagagtagag attcttcctt
    32941 atcatacgat gggaaaatac aagtgggaaa cacttggttt gaaatatcca ttagaaggga
    33001 tcgaaccacc tacaaaagaa cgtgtcgaaa atgctaaacg attgcttcat acagaagagt
    33061 atacaaaata tttaacgcga taggtcgcat cggaaagaag ataagtattg cttctttccg
    33121 tttttttgct attattaaat aggagaaatc acaaaggagc gaatgacatg tcaaaaattt
    33181 tagtttttgg tcaccaaaat ccagatacag atgcaatcgg ggcagccatt gcgttttcac
    33241 acttgcaaaa tgaattaggt aaagaaacag aagcagttgc tctaggtaca cctaatgaag
    33301 aaacacaata cgcattagac cactttcagc tatctgcacc acgagtagta gagtcagcta
    33361 aagcagaaaa tgcagatgtc atgcttgtgg atcataatga attccaacaa agtatttcag
    33421 atatcagcga agtaaatatt ttagctgtag tagaccacca tcgtattgca aactttgaaa
    33481 cagctaatcc attgtactat cgtgcagaac cagttggatg tacaagtacg attattttaa
    33541 aactatataa agaaaaccaa gtaacaattc ctaaagaaat cgcaggaatc atgctctcag
    33601 ctatcatttc agatacattg ttgttcaaat caccaacatg tacgcctgaa gatgaagcag
    33661 ctgcaaaaga acttgcagca atcgctgaag tcgatctaaa cacttatgga cttgaaatgt
    33721 tgaaagcagg aacaaatcta agtgataaat cagctgcggt tcttcttgat ttggatgcaa
    33781 aaagtttccc aatgggcagt tccaacgttc gtatcgctca agtcaataca gtggacctta
    33841 atgaagtgat ggatcgacaa gcagagctag aagcagcaat ggaagatgaa atgaaagcaa
    33901 atggctacga tctgttcgta ttagttgtca caaacatctt agacagtgat tcagaattac
    33961 ttgttgctgg taaaggaaaa gaaaaagtag aagaagcttt ccaaacaact ttgaccaaca
    34021 accgtgcttt gcttaaaggt gtcgtttcac gtaaaaaaca agtcgtacct caattgacgg
    34081 ctgtatttag ctaatcaaat ggtagtatga agcattcaaa atgaatggat aagcgattga
    34141 tataaaaaga aacatagaac atactaaagg ccgaagaaat ggaggagaat aacagtcaat
    34201 tatgactctt gttcttcctc ctttttacgt aaaagccttt taagtaaaat aaaacggata
    34261 gaaaagagaa catatatgaa ctattattca aaaaataagg aaaactttta tcaaaaactg
    34321 accgatgatc cgcttttttc ttcattgacc gattatctat atgaacatcg agaacaagaa
    34381 acgattctaa gagaattgaa aaaagaattt tcgcaaaata aattttccca ttttcttgat
    34441 ttattgatcg atgcaggatt aatcaagagg gaagaacgaa gatatcattt gaactttcca
    34501 atatttgatc ctaaagatta cttgcaacaa gccacttcgg ctgctgaaac catagctgaa
    34561 caattgaaga gactctctgt tgatgaacaa aaattagcga tgggagaagt tatttgggcg
    34621 tattgtttcg aagatgaacg gaaagaagcg tatttttatg gagtaagaaa cagccgagag
    34681 acagaattgc tgaggacaac tgcaggaaat gagaagtacc gttttatcac actttcctct
    34741 atagaacact tcccgcttac tttggctaat tacttttttg tacagaagaa tcagcttcct
    34801 gtaacgaagg cattcaaaga attagccgaa cttattggag acgtaaatga atcgtacttt
    34861 tttgatcaaa tagaagttat cgttgacagg atacgtaaga ataaatataa aaatcgccgg
    34921 cctagtattt tccatcagtc tctacttgtg acgaatacta taaaggaaga agaatcattc
    34981 acattagaat tacctattgt cgaaaaaaac aattttgaga ttgaattacc aacattggat
    35041 ccttcattga caatggagga aatggccttt cttaaacgac aaatcttttc tgagctgagc
    35101 aagaaattta taccgcatgc attttcttat atcaaagaat atggaacagt tttagtgagt
    35161 aaaacctaag agagcagtaa gcaaaaatta tactgactct tgcaggagga gcctttctct
    35221 tcctttatgg ttaaaagaat tatttgagta ttttgcctat ctaccaccta ttagattcac
    35281 tacatggtct ggaaacaaag gaatgcaaaa aaatggtgag aaatgtggaa tgacaaagga
    35341 agtagtgata agaaaagtta gatttttaaa taatcaatat tatgattcag tcaagtatgg
    35401 cattttatgg gaagaactag ctgattagat taggaagtcc atttgataat ccagaatcac
    35461 taagaaacga atggcgagtc attaataatt tatccaatct tgggttattc ttttagattg
    35521 aagtgtttaa aactaaaaaa gcatttatga agtcgtaata gtcaggcttc aaaatgcttt
    35581 ttattttgtt tgttttttct ttgccacata aatacaccta gactgacgat gaagagcata
    35641 tccaacagat ttcctgcgac ttggatcgtc gaagggttgg tcagtggcat taaagcaagg
    35701 acaaaaaagg aaaacaagct gtaaataaaa caggtgacag gaactaatcg aaagaagagc
    35761 ggacatttca attggctaaa aatgagtgaa aaaataaaat aagtaaaaat ggataaaatc
    35821 acgactaaac cattcatgat aaagaagaaa agcgtattgt gattaaacga ataaaaaaga
    35881 attgctaaaa aagaaaacgt tttaaatgca atcaatacgc ttgaagcttc gtttaacgta
    35941 ccaggatgtc gatgaaaaaa ataggcaccg aaataaacag aaaacagtaa gccgcaaaga
    36001 gaagacaaaa tcaaaaaaaa cggattttct tcggcttctg tgatatcaga atgatccaaa
    36061 agcaaccagc ctaatgcact agaaggcgca tgtgaggtct taaggtatga ataacataga
    36121 aaaaataaga tcgtcaaagt tgcaaaaaaa gagcctaaat gtaaaaaatt agtggttcta
    36181 cgttgaattt ccataatatc tccaaattaa attgataagt atataaagta taataacaaa
    36241 ttgatgagta tatgtcgatg ttttttaatc aaaataataa ataaaaactg tctaacattt
    36301 aaaatttaat ttagatatta gacagttttt aagtttgtga gttattttcc ttgtcgtttt
    36361 ctcacttttt tagtccagaa accacctgaa atatattttg gctcataaga aatgataaaa
    36421 gcacgctctt cgatttcttt gatcaggcga tacagttcac gttcgctttt tctaggagaa
    36481 agtatttcga gaactagccg ctcaccttcc agccccattg cagaagtctg tgtcacacca
    36541 taaccgtgtt ctctcagtat acccggcaaa cttttatctt ctgcgctatt tgctggcaaa
    36601 atcgttgtga ccatgatgta tcctaatgcc aatttgtctt caatcaaaat tccgacactg
    36661 atacctactg cgtaacctaa cgcatagaca aataaattca gtggattatc taaacgatct
    36721 aaaacaaggg ataagccgac gatataaatc gtgatctcga ccatactgac aagtggagca
    36781 atcagtcgat agcctttcat tgtcaacatg aaacgtaacg tgttcagagt gatataagca
    36841 aaattaatga ggaagatcat ccccaacatt tttggatcaa tcatttgtaa aactcctttc
    36901 atttatataa caacaatatc aaaaaaaatc gtaagtgaag aaaaattgaa actttataca
    36961 tacaaaaaaa tgataaaatg attataatag aattataaag ataggttagg tgattaaaat
    37021 gaaaacggtc aatcgtgtgt tttgtgccag ccacgtgcta agtctccttc tcagtatata
    37081 cttaggtgtc tattcgattc aacagccaat tgaggtagag aacattgttt ttcagatgtc
    37141 gctgacagat ggtttaattt taagcgtttt tttctttttg atactgttta ttggtaatat
    37201 tttcggtagc gttgccagtt ttttgaattt ttctatttat ccactccttt cgctagcatt
    37261 aggagtggtg ggactttgtt ctctgtgctt atttcccgag ccattcagtc ctgcatttat
    37321 attatttggt acactgaacc tgttccagac aactgttgga gcatggttgc tatggcgatc
    37381 aggaaattta atgaaaatag gtgagtaaca attgaatcca attatattga aacgttctgt
    37441 ccatcatatt ccagtgttag aagttgttct gaaagaatta agagaggaac gtattcccgt
    37501 tgtcatctat tatcacggat ggcagacaag taaggagtta gttttgacgc aaggaagaaa
    37561 aattgcgcag cagggcattc gtgtgatcct gccagatgcg atgaatcatg gggagcgcaa
    37621 acagccggtt tcttctattc catcctatac attttgggat agtatctacg gaaatctgtt
    37681 tgaatttgac acacttatca agcatttaga aaaaagaggt cttctacatg atcagcttgc
    37741 agttggcggg gtttcgatgg ggggaatgac aacatgcgcc ttgttggcta aacatcctca
    37801 tatagtagcg ggcgtgtgtt tgatgggttc ccctgcacct ttggaatatg gaaaagaaat
    37861 cgtaaggcgc gcatcggaat ataattatgt cgtaccaaaa gactattttg atttgatttc
    37921 atgggttaag agctatgatt tgtcattgca gccggaaaag ttagctggac gtcctctgtt
    37981 cttttggcat ggacaagctg acgaaaaaat cccttatata caaacggaac gatttgtaga
    38041 aaaaaatcgg cataagtctt acggaaaaga aattgttttt cgttcctcaa aaacacagcg
    38101 acatatggta caaataccac taatggaaga agcttccact tttttagcaa aaaatttgat
    38161 aaaaaagtat aaataattga aacggaaaat cactcatgac gatagaggat tttccgtttt
    38221 ttattttggg ctataataaa ctctgttagg aaaatttggt ataattgata gtaagaatga
    38281 caacatgtta gaaaggaaga gagcatgccc actttagcag atgttgcaaa acgtgccaat
    38341 gtttcaaaaa tgacggtctc tcgagtcatc aatcatccgg aacaggtgac tgacgaattg
    38401 aaagagttag ttttctcagc aatggctgaa ctggattacc gtccaaatat tgctgcaaaa
    38461 gcacttgtca cgaatcggtc gcagatcatc aaattattta tactggaaga aatcgacaca
    38521 actgagccct attacatgaa tttgctgatg ggaatcgcta aacggatcgg taaagcgcat
    38581 tattctctcc aattagttac aaatgatgcc ttcgacgtcg gttcttgtga tggatacatc
    38641 atcacaggtg tcagaaaaaa agacttcgat tggatcgacc gtttggaaaa accagttgtc
    38701 ttgtttgggg aaaattctga aggatatgat tatgtagaca gcgataatga atatggtaca
    38761 gcaaaagcaa cgcaatatgc cttagaaaga gattatcaaa cgattattta tatcggtatc
    38821 gaagtggacg agccttttga aatttcaagg gaaactggtt atttatctgt gatgaaggaa
    38881 gttgatcttc ctagtaaaat cttgcggttt ccaaatcatt cgacagaagt agaaagatat
    38941 atgacagaaa actgggcaga ttttacagat aaaacttgtt ttgtatgtag ttcagatcgg
    39001 ctagcactag gagcagtaag agggattata aatgctggag gacacttgcc cgaagaattt
    39061 ggtgtgattg gatttgatgg ggtattttta gaccaaatca gttcacctaa attgacaacg
    39121 atcaagcaag atattatacg tatgggtgaa gtatgtggag aaatgttgtt aaaaaaaata
    39181 gaagaaaaag gggcgagtca aggttatcgt cgtttttttc ctgaactagt tatcagagag
    39241 acaacgaatt aagtcttatt actatatata tatttatgaa attcatcgct attttcttaa
    39301 aataatcagc gcattgctac tgaaaacaat gaatctgtgc tattattgag aaaagttcca
    39361 tttttgagaa ggatggtttg aatgaaagaa atcagacgaa aacatttccg ttttcctgcg
    39421 tacgatgatg agttaggcgt aaaattaacc aacgacaaga agcgagtatt atttgacgga
    39481 caagaagatc ttttgactga acagaatcag tccccgtttg aacagatgaa agaatttcat
    39541 ttcacgaaca cgaacaccga gaaaatccgt cgcaagcgtc gtgcaacaaa tggattgaag
    39601 tcagctccgc gtaaggaaaa tttggaaaag cataaaggaa aattaccgga ttataccggc
    39661 cattaccaat cagaaatcac atcgacagga cgaaaaaaac tttttggtga ttcctctcgt
    39721 tctgtcagac aaagtgaaac aaggacagag tccaagcata ctacacgaaa cgcatctgtt
    39781 gacacgaagg tgcgggagcg ttcttatttt gttcctaaat atgtacccgc atcgattatc
    39841 ccagacccta gagaaagcaa aatttctgag gatcatttaa tcgaatcgat gaaaaaaacg
    39901 gatgactctt atctgttgtt cgatacagaa ccgacgccgt atcaagaaaa aagaaatggt
    39961 gagccgtctg tacgaacatt caatcgaaca aaaacgttag aggaaacaaa gcagtcagaa
    40021 gaaaaaaaat cgaaaaaaag ccatggtatc ttagaacgct cattacaagg attgatcgat
    40081 gatcaaggaa actctttggg agaaaatagc tattttaaat aagtgatata caaatttata
    40141 ttcggctaca gttggaaagg aaacgctgcg gcgtttcctt attttatact ctatccgaaa
    40201 gtttatatcg tatcctcaaa tttttttgag tttaagcgaa ttttttctcc caataagtaa
    40261 gaaaataatg gcaaaataaa aaaacaaact attttcgcag atcgtttgca aaaatagttt
    40321 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
    40381 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
    40441 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
    40501 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
    40561 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
    40621 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
    40681 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
    40741 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
    40801 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
    40861 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
    40921 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
    40981 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
    41041 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
    41101 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
    41161 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
    41221 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
    41281 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
    41341 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
    41401 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
    41461 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
    41521 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
    41581 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
    41641 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
    41701 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
    41761 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
    41821 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
    41881 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
    41941 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
    42001 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
    42061 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
    42121 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
    42181 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
    42241 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
    42301 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
    42361 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
    42421 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
    42481 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
    42541 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
    42601 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
    42661 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
    42721 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
    42781 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
    42841 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
    42901 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
    42961 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
    43021 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
    43081 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
    43141 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
    43201 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
    43261 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
    43321 taaattaagc ggaaaaataa cagaggatca aatgattgat atgaattacc aagtcaatgt
    43381 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
    43441 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
    43501 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
    43561 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
    43621 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
    43681 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
    43741 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
    43801 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
    43861 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
    43921 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
    43981 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
    44041 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
    44101 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
    44161 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
    44221 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
    44281 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
    44341 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
    44401 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
    44461 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
    44521 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
    44581 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
    44641 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
    44701 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
    44761 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
    44821 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
    44881 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
    44941 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
    45001 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
    45061 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
    45121 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
    45181 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
    45241 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
    45301 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
    45361 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
    45421 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
    45481 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
    45541 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
    45601 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
    45661 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
    45721 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
    45781 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
    45841 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
    45901 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
    45961 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
    46021 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
    46081 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
    46141 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
    46201 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
    46261 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
    46321 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
    46381 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
    46441 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
    46501 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
    46561 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
    46621 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
    46681 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
    46741 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
    46801 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
    46861 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
    46921 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
    46981 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
    47041 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
    47101 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
    47161 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
    47221 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
    47281 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
    47341 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
    47401 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
    47461 aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa
    47521 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
    47581 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
    47641 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
    47701 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
    47761 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
    47821 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
    47881 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
    47941 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
    48001 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
    48061 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
    48121 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
    48181 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
    48241 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
    48301 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
    48361 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
    48421 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
    48481 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
    48541 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
    48601 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
    48661 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
    48721 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
    48781 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
    48841 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
    48901 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
    48961 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
    49021 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
    49081 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
    49141 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
    49201 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa
    49261 tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt
    49321 gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag
    49381 taagaagaat ctaattggta acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg
    49441 cttgattctt tttgtgcgat ccattctttt gcagagcttg tgcttgctgg agtagcagca
    49501 gtttctgtta cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt
    49561 tgaaccgttt cttgtacaac aggttgttca gcttggactg gtgcttcttg aacggctgct
    49621 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    49681 ta
//
LOCUS       DAHUVV010000012        47460 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.012, whole genome shotgun sequence.
ACCESSION   DAHUVV010000012 DAHUVV010000000
VERSION     DAHUVV010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.012"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(45..779)
                     /locus_tag="NK767_000665"
     CDS             complement(45..779)
                     /locus_tag="NK767_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCH6693679.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="NK767_000666"
     CDS             1106..1516
                     /locus_tag="NK767_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCH6693680.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="NK767_000667"
     CDS             1509..2210
                     /locus_tag="NK767_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCH6693681.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3796
                     /locus_tag="NK767_000668"
     CDS             2210..3796
                     /locus_tag="NK767_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6693682.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3815..4036
                     /locus_tag="NK767_000669"
     CDS             3815..4036
                     /locus_tag="NK767_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCH6693683.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4033..4794
                     /locus_tag="NK767_000670"
     CDS             4033..4794
                     /locus_tag="NK767_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HCH6693684.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5174..5683
                     /locus_tag="NK767_000671"
     CDS             5174..5683
                     /locus_tag="NK767_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCH6693685.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5758..6297)
                     /locus_tag="NK767_000672"
     CDS             complement(5758..6297)
                     /locus_tag="NK767_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCH6693686.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="NK767_000673"
     CDS             complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="NK767_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCH6693687.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7310..8161
                     /locus_tag="NK767_000674"
     CDS             7310..8161
                     /locus_tag="NK767_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCH6693688.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8189..8824
                     /locus_tag="NK767_000675"
     CDS             8189..8824
                     /locus_tag="NK767_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCH6693689.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8844..9617
                     /locus_tag="NK767_000676"
     CDS             8844..9617
                     /locus_tag="NK767_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCH6693690.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10025..10822
                     /locus_tag="NK767_000677"
     CDS             10025..10822
                     /locus_tag="NK767_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCH6693691.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10800..11213
                     /locus_tag="NK767_000678"
     CDS             10800..11213
                     /locus_tag="NK767_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCH6693692.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11197..13842
                     /gene="mutS"
                     /locus_tag="NK767_000679"
     CDS             11197..13842
                     /gene="mutS"
                     /locus_tag="NK767_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCH6693693.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13860..15968
                     /gene="mutL"
                     /locus_tag="NK767_000680"
     CDS             13860..15968
                     /gene="mutL"
                     /locus_tag="NK767_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCH6693694.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15990..16550
                     /gene="maf"
                     /locus_tag="NK767_000681"
     CDS             15990..16550
                     /gene="maf"
                     /locus_tag="NK767_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HCH6693695.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16635..18623)
                     /gene="treP"
                     /locus_tag="NK767_000682"
     CDS             complement(16635..18623)
                     /gene="treP"
                     /locus_tag="NK767_000682"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCH6693696.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18846..19559
                     /gene="treR"
                     /locus_tag="NK767_000683"
     CDS             18846..19559
                     /gene="treR"
                     /locus_tag="NK767_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCH6693697.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19636..20082
                     /gene="msrB"
                     /locus_tag="NK767_000684"
     CDS             19636..20082
                     /gene="msrB"
                     /locus_tag="NK767_000684"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCH6693698.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20252..20854
                     /gene="ruvA"
                     /locus_tag="NK767_000685"
     CDS             20252..20854
                     /gene="ruvA"
                     /locus_tag="NK767_000685"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCH6693699.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20867..21868
                     /gene="ruvB"
                     /locus_tag="NK767_000686"
     CDS             20867..21868
                     /gene="ruvB"
                     /locus_tag="NK767_000686"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCH6693700.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21897..22475
                     /locus_tag="NK767_000687"
     CDS             21897..22475
                     /locus_tag="NK767_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCH6693701.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22527..23009
                     /locus_tag="NK767_000688"
     CDS             22527..23009
                     /locus_tag="NK767_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCH6693702.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23126..23500
                     /locus_tag="NK767_000689"
     CDS             23126..23500
                     /locus_tag="NK767_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693703.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24178..25530)
                     /gene="brnQ"
                     /locus_tag="NK767_000690"
     CDS             complement(24178..25530)
                     /gene="brnQ"
                     /locus_tag="NK767_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCH6693704.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25677..26267
                     /locus_tag="NK767_000691"
     CDS             25677..26267
                     /locus_tag="NK767_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCH6693705.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26395..27744)
                     /locus_tag="NK767_000692"
     CDS             complement(26395..27744)
                     /locus_tag="NK767_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6693706.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27912..28652)
                     /locus_tag="NK767_000693"
     CDS             complement(27912..28652)
                     /locus_tag="NK767_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCH6693707.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28665..30257)
                     /locus_tag="NK767_000694"
     CDS             complement(28665..30257)
                     /locus_tag="NK767_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCH6693708.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30825..31121)
                     /locus_tag="NK767_000695"
     CDS             complement(30825..31121)
                     /locus_tag="NK767_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693709.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31297..32022
                     /locus_tag="NK767_000696"
     CDS             31297..32022
                     /locus_tag="NK767_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCH6693710.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32015..32857
                     /locus_tag="NK767_000697"
     CDS             32015..32857
                     /locus_tag="NK767_000697"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCH6693711.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32860..33381
                     /locus_tag="NK767_000698"
     CDS             32860..33381
                     /locus_tag="NK767_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HCH6693712.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33634..34197)
                     /locus_tag="NK767_000699"
     CDS             complement(33634..34197)
                     /locus_tag="NK767_000699"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCH6693713.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34448..34717
                     /gene="rpsO"
                     /locus_tag="NK767_000700"
     CDS             34448..34717
                     /gene="rpsO"
                     /locus_tag="NK767_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCH6693714.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34905..37031
                     /gene="pnp"
                     /locus_tag="NK767_000701"
     CDS             34905..37031
                     /gene="pnp"
                     /locus_tag="NK767_000701"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCH6693715.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37541..37807
                     /locus_tag="NK767_000702"
     CDS             37541..37807
                     /locus_tag="NK767_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCH6693716.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37984..39942
                     /locus_tag="NK767_000703"
     CDS             37984..39942
                     /locus_tag="NK767_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCH6693717.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40296..41597
                     /locus_tag="NK767_000704"
     CDS             40296..41597
                     /locus_tag="NK767_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCH6693718.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41727..43097)
                     /locus_tag="NK767_000705"
     CDS             complement(41727..43097)
                     /locus_tag="NK767_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCH6693719.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVNPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43279..43602)
                     /locus_tag="NK767_000706"
     CDS             complement(43279..43602)
                     /locus_tag="NK767_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCH6693720.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43927..44535)
                     /locus_tag="NK767_000707"
     CDS             complement(43927..44535)
                     /locus_tag="NK767_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCH6693721.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44865..45581
                     /locus_tag="NK767_000708"
     CDS             44865..45581
                     /locus_tag="NK767_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HCH6693722.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45594..46289
                     /locus_tag="NK767_000709"
     CDS             45594..46289
                     /locus_tag="NK767_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCH6693723.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46373..47002
                     /gene="udk"
                     /locus_tag="NK767_000710"
     CDS             46373..47002
                     /gene="udk"
                     /locus_tag="NK767_000710"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCH6693724.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15383 a         8787 c         9878 g        13412 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca aaagcaatcg
     2101 agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac ggaatattga
     2161 ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgactgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaacaaaa
     3781 actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa ttttaccaga
     3841 attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac tttatgttgt
     3901 tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat tgatcaaaac
     3961 aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag cagatgattg
     4021 ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt atttatcaca
     4081 ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca agatgctttt
     4141 gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg atttcctgaa
     4201 acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca agcggtatcg
     4261 aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg gatattggat
     4321 gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta tgagacgaat
     4381 gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg ccaaaaaagc
     4441 ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg tgggatcgct
     4501 tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct ggattacgca
     4561 ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc aatgaatgcg
     4621 gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac accagtcaag
     4681 cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc tcctcaagca
     4741 agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa ataaccttgt
     4801 gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg atgataaaaa
     4861 aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa ttctttttaa
     4921 taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa acgatattca
     4981 aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag ctatttttat
     5041 ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc gacacttcag
     5101 ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt atcttgcaaa
     5161 ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga caacgacaga
     5221 tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg ttttgtctgt
     5281 ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc tgtccttgta
     5341 taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata ttgcttctcc
     5401 tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat tgcttatcaa
     5461 ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga cagcatgcgt
     5521 atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc aactaccttt
     5581 tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg tcggtgggat
     5641 aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg tttaatttaa
     5701 aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct tttttgatta
     5761 atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat aagctgctcc
     5821 agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa atgcagttgt
     5881 aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg cagcaagaga
     5941 gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt cgatcatcca
     6001 accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg gcccaaataa
     6061 aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc caagtaaaag
     6121 ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg ttaatccaat
     6181 agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct gagaaaggac
     6241 agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc ggttcataac
     6301 gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa acaaagtgat
     6361 tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc ggtctttttc
     6421 aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga gcttcgtcga
     6481 tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc aatttttcag
     6541 cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct tttactgtta
     6601 cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg tggtatgaag
     6661 ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat tgacgtgctt
     6721 gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt tgttctagta
     6781 agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt acgaacaagt
     6841 tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt tcagttaatt
     6901 gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct ggtgcgtaag
     6961 ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga cgagaaattt
     7021 tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat aagtttttgg
     7081 agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat cttcaccttt
     7141 gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat gagttattat
     7201 ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca cgaatgtcaa
     7261 gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa tgagagggta
     7321 tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc cggtatcacc
     7381 attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg aaaacagctt
     7441 ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag gaacggctgt
     7501 ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa agattttata
     7561 cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg aattaggaac
     7621 atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt taagtaaggc
     7681 agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat tatgtggatt
     7741 tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta tgttagaatc
     7801 aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga tgtttgcgaa
     7861 tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg tcggaaaagt
     7921 gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac acgtgatagc
     7981 gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg atggaatgac
     8041 agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata ttggtggcgg
     8101 tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct atgtggacta
     8161 aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa aaaatgagga
     8221 aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg gagtacctaa
     8281 gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt tatctatttc
     8341 tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt atcagtatgt
     8401 agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact tagtcgtatt
     8461 gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga gaaaagtata
     8521 cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat tgtttgaaaa
     8581 aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt ataaacagca
     8641 aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa aggaattctt
     8701 tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg ccgattgggt
     8761 aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa gtaagtattt
     8821 ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat attgatggaa
     8881 cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg gaaaaattac
     8941 gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat gcgcaagaga
     9001 tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc ggttttttag
     9061 accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg ttcgttcaag
     9121 aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc aagcgttctt
     9181 cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct gtcttgcctg
     9241 aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca tttttcgatc
     9301 aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc tggcatgaga
     9361 acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg cacttagcag
     9421 aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa aacgaccgtg
     9481 aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca gaagtacaag
     9541 cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag gcattgaaag
     9601 agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt aataagccca
     9661 gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc gcagatgcaa
     9721 gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc aagaaataag
     9781 aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat ttctgaagaa
     9841 gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg tcatagtccc
     9901 atttttttat tttatttttc aaaactaaga aataccagga aagagttcct tttgtttgac
     9961 cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag aagaggaatt
    10021 tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag agatgattac
    10081 tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg taaatggtga
    10141 gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt tgcaagacgg
    10201 cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct ttgagtttgt
    10261 aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc ctggacaagg
    10321 gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc aggctcgttc
    10381 ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag aagaagccag
    10441 aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg aaaaacaagc
    10501 aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata cacacgttca
    10561 gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg ttgggatgac
    10621 aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa agtttctgac
    10681 tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa gcggctgtat
    10741 cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc agatcaacga
    10801 tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag ccggaaattc
    10861 aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc attcgctaca
    10921 aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga gaaaaaatca
    10981 aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca gcagaaaaag
    11041 aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg cttgttgtag
    11101 cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca actatgatac
    11161 aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac taaaaatact
    11221 cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt tttgttctat
    11281 cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc acagcttttg
    11341 gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat gtgcggtgta
    11401 cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta taaagtagcg
    11461 atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg cgaagtgatc
    11521 cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa agacaacaat
    11581 tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc cgatttaagt
    11641 acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa tgaagcctct
    11701 gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct tcgtgaacag
    11761 ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga aaacgcggaa
    11821 tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac cggcaaacta
    11881 ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa agcagtagaa
    11941 taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct agaattaagc
    12001 caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga tgaaacaaaa
    12061 acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat ccaggcaaaa
    12121 caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt tgaacgtatc
    12181 gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg tcgagtagct
    12241 ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga acaagtgcca
    12301 atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct gctttcagaa
    12361 atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga tgatccgccg
    12421 ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct cgatatgtac
    12481 cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa agagcgtcaa
    12541 gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta ttatatagaa
    12601 attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa acaaacttta
    12661 gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca aatcctcgaa
    12721 gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag agaagaagtc
    12781 aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt agatgtgcta
    12841 caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact tgtcagtgac
    12901 aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt attaggacat
    12961 caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt gctgattaca
    13021 ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac tgttctgatg
    13081 gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt tgatcggatt
    13141 tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt tatggtagag
    13201 atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat tttgtttgac
    13261 gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc aatcatcgaa
    13321 tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca tgaactgacg
    13381 gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt tgaaaaagac
    13441 ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag ctacggcata
    13501 catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc agatatcctg
    13561 catacgctag aaaatggcga taacggacac catactgttc cagctgaagt agaagagaaa
    13621 actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa tcaagaccat
    13681 atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga attatccgaa
    13741 aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat gacgccaatg
    13801 gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg cggatatcga
    13861 tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg ggagaagtag
    13921 tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac gccggcagta
    13981 ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg attgataacg
    14041 gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg acaagtaaga
    14101 tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg gaagctttgc
    14161 caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa gaaaaacaag
    14221 gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca ccactgcgtc
    14281 aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc ttgaaatacg
    14341 tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga ctggcattga
    14401 gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg gcaactgctg
    14461 gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc gcaaaaaaaa
    14521 tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt tcattgcctg
    14581 aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt tttatcaaga
    14641 acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg gtcggtcgtt
    14701 tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta aatgttcatc
    14761 cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta attagtaacg
    14821 ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat aatctacggt
    14881 ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat ttgtcctacg
    14941 cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga gagacaggta
    15001 cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat aagtctgtgg
    15061 aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca gaaaaaacag
    15121 cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa acagtggaaa
    15181 aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat gaagaacaag
    15241 cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca ctccatcctg
    15301 aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag aaattagaaa
    15361 cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg cacggaacgt
    15421 atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct gcccaagagc
    15481 gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat ctgcaagaat
    15541 tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta aaagagcaaa
    15601 aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac agctttattg
    15661 tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt cgggagatga
    15721 tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa gcaacagcaa
    15781 tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa gcacaagcac
    15841 gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc catggccgtc
    15901 cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga atccaagacc
    15961 cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc caatcaccaa
    16021 gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct gctgatatcg
    16081 atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg gctgaagaaa
    16141 aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt gccagtgaca
    16201 cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct gaggctgaat
    16261 tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt gttttaaaaa
    16321 caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat cctttgacag
    16381 acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca ggggcatacg
    16441 gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat tacagtatcg
    16501 taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa acaaaaaagc
    16561 ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt gaaaagatta
    16621 cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc cgggcgaaca
    16681 agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa agcaacaata
    16741 atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc aatcagggtc
    16801 tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc ctttagttca
    16861 acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag aataccaatg
    16921 gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata gactttgccg
    16981 tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac tgggtcattt
    17041 acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc ttttgttgca
    17101 actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt tgcgccattt
    17161 gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat cttattcaaa
    17221 attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc aatgatcatt
    17281 gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg caaaccgcct
    17341 actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc taatccagag
    17401 ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa catagcaggt
    17461 tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc ttctttttta
    17521 ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat atttgataaa
    17581 gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat atcgatggca
    17641 ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc gaatacggca
    17701 ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga gatccagctt
    17761 ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt tggtaacaat
    17821 gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg gaagaagatt
    17881 tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc ttgataacca
    17941 atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc ggctgtggaa
    18001 gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa aacgattcct
    18061 aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac tggaagaaaa
    18121 tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca aaacttcgag
    18181 tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac atttctgaat
    18241 ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat ttctgctaaa
    18301 acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt tccttgttct
    18361 ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt agaaacatcg
    18421 tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac gctaggtata
    18481 tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg catacgagtc
    18541 gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa cagcttctcg
    18601 gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg tctatgcaag
    18661 ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt attaaaaagt
    18721 acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa aaataggaac
    18781 attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg agtgagggaa
    18841 aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt ttagcgaaag
    18901 aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt tatggtgttt
    18961 cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat attcaaaaaa
    19021 aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct atttctggat
    19081 tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg atcatccata
    19141 cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg gagatcggtg
    19201 cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt atattagaca
    19261 ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca caaggatcga
    19321 tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa aaggagatca
    19381 cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat gacacttatg
    19441 cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag tattctgagt
    19501 cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga aaagtgtaaa
    19561 tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga taggatcaca
    19621 aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact gaaagaaaag
    19681 ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc atttagcggg
    19741 aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg tgaaccgtta
    19801 ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag caaaccaata
    19861 acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg aatagaagtt
    19921 cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc agtagatcaa
    19981 ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata tgaaaagatg
    20041 gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata cacaaagagc
    20101 ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg attgctattt
    20161 gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca ataaagggaa
    20221 cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag gtgtggttac
    20281 ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc agatcgcact
    20341 aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt atgtccatca
    20401 ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag aaaagcaact
    20461 gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg caatcatggc
    20521 aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca cttatttgac
    20581 gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc taaaaggaaa
    20641 attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa acacaaaaga
    20701 cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca gtgatcgaga
    20761 aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg atgagtatct
    20821 acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga cagatgaata
    20881 cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc ttcgaccaca
    20941 gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga tttacatcga
    21001 agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc ctcctggatt
    21061 aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata ttcgtacgac
    21121 gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg aactggaagc
    21181 aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag aagagatgct
    21241 ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc caaccgcaca
    21301 tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac gggcaggtat
    21361 gctttctgca cctttacgag atcgatttgg catcatttct catatggaat attatgacga
    21421 acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag agatcattga
    21481 agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg ctaatcgctt
    21541 gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg atcgtcctat
    21601 agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt atgttgacca
    21661 gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt taagtacgct
    21721 ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac cgtatctgat
    21781 ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt atgcctatga
    21841 acattttggt tacgactatc atatatgatt tttgctttta gattggagga aaatgaatga
    21901 gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg tgccaaacgc
    21961 aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc aatcgacaaa
    22021 cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat tgtgaggatg
    22081 cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag caagcattga
    22141 aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg ctttctgacc
    22201 cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc aagatttttg
    22261 aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat gctcgttttt
    22321 tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat gaagaaacac
    22381 cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta tattcttatc
    22441 gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg ttggaaaagc
    22501 aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt ttgggaaata
    22561 tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta gaaggaaggg
    22621 aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga aagacaccgc
    22681 accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat atggttgctc
    22741 ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg gatcaagaaa
    22801 atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc catttgtttt
    22861 taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat accaataact
    22921 ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat atttggattg
    22981 atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag gcgatgttag
    23041 agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa gtaacacttt
    23101 cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa tgaagaagct
    23161 tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa atgacactga
    23221 agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc aaaaagctgc
    23281 tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta gcaagtatga
    23341 aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga tgatccgcct
    23401 acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa aaataacaat
    23461 cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag atagaaaagc
    23521 acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc tttaagctga
    23581 tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt gggttgatta
    23641 gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct agccggggag
    23701 ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt tggtatgctt
    23761 atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt tgttaagttt
    23821 ttttgcgaaa actattttta tatcatatat atgtctaaaa tctatattat aaaagaaatg
    23881 ttgtttgatc agcttctctt cggtattagt tcaaaacaaa acggcaaagt atatggagct
    23941 actttgccgt tttgtttctc taatactcag gtcagtttat agacgctcaa taaacggtgt
    24001 tcaaaagcca gtttctcggt attaagtcaa atttatgcaa aaatgtgaga agccattttc
    24061 aaaaaatatt tcttaatact cgaaactgag cggctttttc acgacctctc aataaacagt
    24121 gttcaagaga cagcctatcg gcaataaggc tgaacgtctc tttcacaacc tcttttatca
    24181 atctgctact tcttctttct tagcgaaact ccaaatcagt ccgataacaa aaccgataac
    24241 tgctggtacg atccacccca tccccaattt gaaaaacggc aagtagcttt ccgcaacata
    24301 taaaagattt tgaacaatag gtgtttgttt gatagtttct gggcaagcgt tcaacccatc
    24361 aaaaatcgaa gcaatcaatg taaaataagt agtcattcga taaacagccg tacgctgctt
    24421 aaacaaagga ccaactaaca ctagtaaaat cagtgtcatt gctaatggat agatgaacat
    24481 caaaacaggc aacgaaaatt gaatgatatt cgtcaagcct acgtttgccg ctaaacaagg
    24541 aaggatacta gcaagtgcaa caaagaatgc gtaatttctt tgcggaaaca aatctgtgaa
    24601 tgtttctgca aaagctgtaa tcaaaccaat accggtcttc aagcaagcta atataacgat
    24661 aaatgctaat aagatactgc cataagtacc taaataatga tcagcaatct gagccagcgt
    24721 gatcccgcca ttttcactta gagcaaagct acctaagctc atagcaccta aataagcgag
    24781 caaagtataa atgattccca tcaatataat gctgatcgca ccagatttga tcgtgtcttt
    24841 agcgatttca gcaggttttg ttacacccat gttttggatt gttgtgacaa tgatgatccc
    24901 aaatgctaaa gcagcaagcg catctaatgt gttatatcct tgagtaaacc ctgtgaaaaa
    24961 agcatgctct tgataatttg gctgaatagg cgcttgattt acagaaccta gtggattcaa
    25021 aaaagcaagg agaagcaaag cacctaataa aaccaaaaat gcaggattca ggaactttcc
    25081 aacataatct aatatctttg tcggttttct tgaaaaccac caagccagca caaaaaatac
    25141 aatactgaaa attgctagat atagcgtctg tctgctagga tcgataaatg gtgctaaacc
    25201 aatctcgaaa gatgtggttg caaggcgagg taaagcaaaa aacggtccga taactaagta
    25261 aagcaagatg gtaaaaacca tcgcatatgt ttgatttatc cgttttgcta attcatatac
    25321 acctgagctt ttcgatacac caatggcaat cacccctaag aagggaagac cgataccggt
    25381 aaccaagaaa cctagattcg cccaaaaggt agcagcgcct gcttcttgac ccaaatgaac
    25441 gggaaagata agatttccag caccaaaaaa tagaccgaaa agcatggagc ctataaaaag
    25501 atagtcttta aatgataatt tttttgtcat acataactcc tttacggttt ttttaaaagt
    25561 aacaaaattt cgaaaaatat gcaacaattt tcagaaaatt tcctatataa aacgacggtt
    25621 atttaataac gtaagtgata aaataaaagc gctgaatcat ctgaggagga atcatcgtga
    25681 caaagtcgag attagaagca tttactgatg gagtggtagc cattgttttg agagttcttg
    25741 tattagagat caagatccct aatacatcta gctttcattc tttatgggtc atccgaaata
    25801 cattattagc ttataccatc agctttatat ttgttgcggt gatctgggtg aaccatcatc
    25861 gtatatttca gatggcagaa cgaattaatt accgagtggt ctggtcgaat atctttttgc
    25921 tcttttggct gactttatgt ccttctgtta ctagttgggt cgggcgtaac cctgaagctt
    25981 tttggcctga gatggcttat gtcttagtat acacgatgtg gagtttttct tttggtatct
    26041 tgatgaggca aatcatgaaa gcaaataaac cagattccca tgtcgtgaga gttttgtcaa
    26101 aggaccgtcg aagtcttatc tccatgatga ttaacttatg cttgattgcc ggtgtctttt
    26161 ttgtaccgtt gatcgggtta ttcggacgtt tttttgtatc tggtatttgg attttttcct
    26221 ataaaaaagc agatgcctat tatcagcggt tgtttccggg gaaatagaaa agaggctggg
    26281 acagaagctt ttaactccaa gaaataagcc gaaatttcac aaaaatttaa gaagcaattt
    26341 tcgtaaattc cttcttattt ctcgaagctg accacttctg tcacaactac tttcttactt
    26401 cttacgttca tatttcatat gatagattag taatagaagc aagaaccaca caggtgttag
    26461 taataaagcg attctcgtat cttttcctag gaacaagacg acacacacaa aggccatgta
    26521 aagaaatacc agataattac taaatgggta caacggcatt ttaaatgaat gagctttttt
    26581 gccgaggata cttttacgat atttcatgtg cgtgtagaca agcatactcc agataaacaa
    26641 gaaacaagta gtggcaatac tgcttactag tgtgaatact tcactaggca tcacgtaatt
    26701 caagataaca gaaatgaaaa tgacgatagt agaaaatgtc aaagcattcc ctggcacatg
    26761 atgtgttgtt aactttttga attttttagg ggcactgcct tcttgcgcta aagaacgaag
    26821 catacgtcct gtactatata ttgcactgtt gcaggcagaa gctgctgcac ttaatacgac
    26881 taaattgatc aaggaagcag caatcgtgat cccaatttcc gaaaatactt ctacaaatgg
    26941 actgttatct ggattcaagc tgttccatgg atagattgcc ataatgacaa aaagagatcc
    27001 taagtaaaat aagatgatcc tgataggaat attattgata gcttccggca acactttttc
    27061 aggattttct gtttctccag cgactaatcc aactaattct atcccagcaa aagcaaaggt
    27121 taccatttgg aaagctaaga taaatccttt tgcaccagtt ggaaagaatc cgccatgaga
    27181 ccatagattc gtgatactgg ctgtgccaat atctgtcgta tagtgggtga ggatcatata
    27241 agctcctacg aggataaagg caatgatcgc tacaactttg atcaatgcaa accaaaactc
    27301 taactcacca aacaatgaga cagccaccat attcaagcaa agaagaagac ctaatgcaat
    27361 cagttcagga agccactgag gaacaccagg cgcccagtag cgtacataca ttccgatggc
    27421 tgttaaatcg gccattgcaa tcgataccca gcagaaccaa tacgtccagc cagtgatgaa
    27481 agccgctttt ttgccaagat attctgcaac aaaatccaga aaagaatggt tattggtgtt
    27541 tgataacaga agttcgccta acgcacgcat aatgaaaaaa cagatgcatc cagtaataag
    27601 ataggcgagc aatatcgaag ggccagctag ctggatagac tttccggaac ctaaaaacaa
    27661 ccccgttcca atcgctccgc caatagaaat cagttggaca tgtctatttt tcagtccacg
    27721 ctgcagttct tgctcttcat tcatttttaa atcatcctaa ctaaaaaagt ttaattactt
    27781 ccatattaaa agagttttct gaaaaacaca actaagattc caaaattttc agaaatttac
    27841 cttttatgag taattcataa aaaagaactg cggtaaacac cgcagctctt tgtcccaatc
    27901 agctattgat cctaagcatt taaaatacgt gaaaggaatt cttttgtacg ttcttctttc
    27961 ggatggacaa agatatcttc tggtgtacct tcttctgcaa tcacaccttt atccataaaa
    28021 atgacacggt cagatacatc ttttgcaaat tccatctcat gcgtcacgat aagcattgtc
    28081 aagccagtat gtgctaagtc tttgatcgta ttaagcactt cgccaaccat ttcagggtct
    28141 aacgccgaag taggttcgtc gaatagcaat acatctggat tcattgataa tgcccgagca
    28201 attgcgacac gctgtttctg tccaccagat aattgtgctg gtttggcttc gatataacga
    28261 tccataccaa ctttttccag attttcgatt gctacttttt tggcagtttc tttatctcgt
    28321 ttcaggacag ttgtttgacc agacatacag ttttccaaga cattcatatt gttgaacagg
    28381 ttgaaggatt ggaaaaccat acctagattc gttcgatatt taggtaaatt atatttagga
    28441 gctaatacat tttctccatt ataaatgatc tcgccgcctg ttggtttttc cagtaaattg
    28501 atacagcgca agaaagtaga tttaccagaa ccggaagaac cgatgatcgt cacaacttca
    28561 cctttattga ctgttacgct gatatctttc aatacttcat tttcaccgaa gctttttctt
    28621 agatgtttga tttcaataat tttttcattc atgattcttc ctccttattc ctgcaaattt
    28681 gaattgtcga tttcttcaag cttggtataa gctgaaggac catcgatttt cttttctacc
    28741 gcccgcagga ttctggtgat cgtaaaggtc atgactaagt agatgatccc gacgatcgta
    28801 aatgtttgga agaattggaa attcgcaccg gaagcagagt ttccttggaa gaacaaatca
    28861 gctacgccga ttacacttaa aactgctgta tctttgatat tgataacaaa ctcatttcct
    28921 gttgctggca agatattccg taaaacttgt ggaataacaa cttttctcat cgtttggcca
    28981 tgggtcatac caatggcttg cgcagcttcg aactgccctt catctactgc aaaaatcccc
    29041 ccacgaacga tctctgtcat gtaagctcca gtattgagag agacaataaa caaagcggca
    29101 atcgttcggt tcaatgaaat gccaaaagcg agtgccagac cgtagaagat gaccattgct
    29161 tgtaccatca tcggtgttcc gcggaatact tcgacataga tagaaagaag tgcatttccg
    29221 attttttgga agaagcgagt cagtgcattt cctgattcag gtaccgaacg gaagacacca
    29281 atcagtaatc caagtgtagt accgacaact gtcccgatca atgcgatgaa caaggtaagt
    29341 ccagcacctc gtaagaacat attgccgtat tgtttccaaa tcgtagtgaa atcagaaatg
    29401 atgccaggtt tttcttcact atcagttgca gcaggctgat ctttgactgc ttggtccata
    29461 attttggttc gttcatcgtt agagatgcca gaaagaatct ggtttacttt ttggatatcc
    29521 gggtcgcctt tacgcatacc aacagaaatc tgaacatctt ccgggtttgt ctggaagcca
    29581 tctgcaccgc tgaattcaag catttccaag tctttgttca cactagttgc cgttacacct
    29641 tccggacgtt cgctgacata accatcgatc attccagaag ctaaagctgt tctcattgct
    29701 gagaaattat ccattgcttg ttgtttctgc acatctggta tctgatcgat cacagtatag
    29761 tggaatgtat ttaactgagc agtaatcttt gctccagaaa gatctgctaa actttttgct
    29821 tttgcatatt tgctatcttt ttgaactact actactaatt gtgattcgta ataaggatcg
    29881 gtaaaatcaa tttcttttcg tcgttcagca gtagggctca ttccggcgat gatcgcgtcg
    29941 atcttgcctg attgaagggc aggagccaga ccatcccatt tcgtttggac gatgactaat
    30001 tttttcccta aaccatctgc tactttttta gcgatttgaa catcataacc gccggcgtaa
    30061 ctattttggc cttgaattgg caaggcatta ttctcatctg tttgttgaga ccagttgaag
    30121 ggtgcatacc cagcctccat acctacgcga aactcatcgc tcggtgtttt ttcatctgca
    30181 tttgccagag atcccacact gaatagtgag aagacaaaag caaatagtac ggcaactgaa
    30241 accatatttt ttttcatttt tttctccctt tctgattata tgatacacga gcggtctaga
    30301 tgaaatgcgg taaagcttta tctgaaaaaa taaaaagcga ccgatttata gatataaaaa
    30361 ggccgctaaa attccataag gtgataaacc tctcacaaaa catagcgcaa cttagcatca
    30421 ctgctaagac agtccgcaag ctcttaactt gcgtcccaac atacactcca agcaaaaaag
    30481 tgcacatttc ggcgatcctc cctattcctg cttctccatg tttgcttcac tccacagggt
    30541 taatgattct cgcgacctct acctcattga ttgaggcatc tcgtattcag ttatttatac
    30601 aaaaaaagtt tacgatactc gagaggcttt gtcaatctat aactgctgtt ttttgaaaaa
    30661 atactgataa atatagaaat ctttactttt accgaaaaaa caatcaaaat gtgaatcatc
    30721 tatccattgc aaacgatagt ataattatac acaaaaatgt atgtaataca ataaaaaaag
    30781 aactagaaca gaaaatttcc tgtgtccagt tccatctcga tcatttaagc tgccgctttc
    30841 tttggctcac gtcctaggat cgcttgcaaa agaacaacga cgccataaat ggctaatatt
    30901 agataaatca aacttgagta ttgggtgatt ccccagcccc atgttcgata tacggctaat
    30961 gtaatggcta aaacaattgc ggcaatcacg ttgaataaag caaaagccca aagatttcca
    31021 ttttctttgc gtacaagata aaaaacagag aaataaacgc ttaacgctaa ccaggcgata
    31081 caagccagaa taacgcccca ataaataaag aatggtaaca tatcgattca cctcactttt
    31141 atacagcagt attgtaacac gttttcattc ttttgtcagt gaacattgtg ataaattgtt
    31201 caaatgccca taacactttt ttattcccat ttttctgact tttcaacaat aaatttgttc
    31261 gagtatgggc tataatggaa agtgaggtga caaaacatga aacaattaac agtagacgaa
    31321 gcagcaaaag agatattagc ttatcagcaa attacatttt tgaccggtgc aggggtatcc
    31381 acaccttctg gagtgccaga ctatcgatct ttgtcaggaa tctaccacgg gatggagcag
    31441 ccggaatact tgctgagtca tcaagcaatg gtgaatgaac cggataaatt ctatcaattt
    31501 gtcaaaaaga tttatcatcc tgaagcaaaa cccaatatca ttcatttgga aatggcacat
    31561 ctagcgaaag aaaaaaatgt ttgggttgtt tcccaaaaca tcgacggttt gcacgcaaag
    31621 gctaaaagcc cgcaactcgt caattttcat ggaagtttgt atcaatgtca ttgtcgaaaa
    31681 tgtcaacaaa cggtagactg gaaagaatat cttcacagcg acaaacatca agcatgcgga
    31741 ggacaaatac gcccagacat cgtgttatat ggtgaagggt ttcaagatgg agtcatggag
    31801 caggcggctt atgccgtcag ccaagcagag ctgatcgtca ttgttggaac gagttttcaa
    31861 gttcatccgt tttgtgatct ggtacagttt cgccagccgt cagccaaaat cttagtaatc
    31921 aatcaaacgc ctgtttattt agcggagcct tatacgttta ttcaagaaga cggagcggtt
    31981 gttttcaaaa aaatacaaga attaggagca gattatgact gaattcaaaa ccgaaaaaga
    32041 aaaaatgatt gcaggcgagc tttattttgc caatgatcct gagctggttg ccgaccgtat
    32101 gtttgcaaga agtcaaagcc aaatcatcaa ccaagcagaa tcagcggaac tgcgatccca
    32161 attgttgaaa gagacttttg gccgtacagg gaaaaaaatc tatatggagc cagttatcaa
    32221 ttttgattac ggatataata tatttgttgg ggagaatttt tatgcaaatt tcaattgtac
    32281 atttttagat gtcagcacga ttgaaatcgg tgataactgc atgttcgctc cgaatgtcca
    32341 gctttacacg gcgacgcatc cactacatcc agtgaaacgt aacagcggtc tggagtacgc
    32401 aaaacccatt aaaatcggta ataatgtttg gttaggcgga ggagttatcg tcacgccggg
    32461 tgtcactcta ggggacaacg tagtagtcgg cgcaggttcg gttgtgacca aatcttttcc
    32521 cgacaatgta gtgattgcag gaaatcctgc tcgtatcatc aagacagtag aggaagaact
    32581 aacagaagag tctcttgaaa cattacgtca taagatcgat atgatcgatc gagagttgat
    32641 tgctttatta gaaaaaagaa tggatacggt aactaaaata ggacaagtga aaaaagaaga
    32701 aaaccaagca gtttatgatg caaaaagaga gcagcaagta ttggataaag ttgttagtct
    32761 gctgaaaaat aagggatata aggaaacaat aacagatacg tatgttgatt tgatgaaaca
    32821 ctcgcgcgaa taccaaaaca aaatgaagga ggaataagtg tgatcgaagg agagacacgg
    32881 cgaaaacaaa tcatgcaaac cttggcaaat acagaaaaac cagtgagtgc tagcaagttt
    32941 gcgaaaactt ttggagtcag ccgtcagatc attgtaggcg atattgcttt actacgagct
    33001 gcaggtaaaa gtatcgtggc aacagcaaga ggttatatcc tcgaaacaga agaaaccaat
    33061 actgggcaca tcagtaaaat cgctgtccag catggaaaag atcaaacaga agaagaactg
    33121 agactaatcg ttgaaaatgg tggtgaaatc attgatgtaa ttgtggaaca tcccctttac
    33181 ggtgagttag caggaacttt gcacatcaaa acgccacaag atatcgattc ctttatgaga
    33241 cgttatcaaa aaagtaatgc tactttgtta tccgaattaa caggcggcat ccatctgcat
    33301 acgatccgct acccagaaaa gcatatgctg aaaaacatca aacaaaaact agctgaagca
    33361 ggaatcctct acgaaggata acatcgtagc tgctactttt tatcctagaa actattctat
    33421 catctctgag ataaacaccc atatggaaga aataagaaaa aataagaaaa gatgcgtatc
    33481 caatacacag aattttttgg aattgcgatt tctagatgtc tatctaaatt tcgattagtg
    33541 gagaaacata aaaaagggaa gtgcagcttt tgagcactcc ccttttttat aaagctttct
    33601 tgtagcatag tatcgaagga acctgccaat tctctactcg atcaccaata cgccttcttt
    33661 tagtgcaaat ggattttgat cgttgatgtg gtcatagaac atcacaccgt tgatatggtc
    33721 gatttcatgt tgaacgacga tcgattcata attcttcaaa cgaatcttgt gtttttcacc
    33781 attcatatca taataagaca cagtgatttt ggcatggcga acaacatatc ctggtacttc
    33841 tcgatcaaca gataaacagc cttcaccttc ccctaaacaa gcatcttgaa cagaatggct
    33901 caaaatttta ggattataca taacagtact tagagaaggt ccatctgcct ctgggtcagg
    33961 acttggtacg tgaacagcaa tgattctttt agaaatatct aattgaggag cagctaagcc
    34021 aacaccgcct cgtaagtgta gttcttcagc tttgattgga tcttggctgt tttttaagaa
    34081 ctcaagcatc tcttttccta aagagatatc ttcttcgctt aatggaagag aaacttcttt
    34141 tgcaacttcg cgtaaggtcg gatttccttc acgaatgata tcatccattg taatcatgga
    34201 atatgtaccc ctttcttaaa agactgtgtt tcaagtctat tttcgcatta tagtttacca
    34261 caaaaatgga aaagtagtag taagattgtt tgaaaactac cttttcttaa tgattaacta
    34321 cttgattttc aacccggttt caggtaaagt atagctgtgt gctctaggca catgacctgc
    34381 cacttggtta agcgaatcac caacgtttta ctcagtcgca ggggaataat cgaaagaggt
    34441 gaaagaaatg gcaatttcaa aagaacgcaa gaatgaaatc atcaacgaat acgctcgcca
    34501 tgaaggggat actggttctc cagaagtaca aatcgctgta ttaactgaag aaatcaacca
    34561 cttgaatgaa catgcacgcg tgcacaaaaa agaccatcat tcataccgcg gcctaatgaa
    34621 aaaagttggt caccgccgta acttgttagc ttacttacgt aaaactgacg ttcaacgtta
    34681 ccgcgaattg atcaaacgtt taggcttgcg tcgttaattt tagtcaaaaa gagtgggatt
    34741 cacatgaatc tcactttttt tactcgtata tcaattaagt aaaattccgt gatcaagcta
    34801 gatggactcc tactaaccaa atgaaagctg ctcaaaagac ggctttcggc agttttcatt
    34861 tgtttagtag cgtcttgctt tcacgagaag gaaaaggaga agttatgtca gaaaaacaag
    34921 tattcaaaac gacatggggc ggccgtccct tacaaatcga agtcggtcag ctagcaaaac
    34981 aagcaaacgg agccgtttta gtccgttatg gagatacagt agtcttaagt gcagctgtgg
    35041 cctctaaaga agcaaaagat acagactttt tcccattaac gatcaattat gaagaaaaaa
    35101 tgtatgcagc agggaaaatc cctggaggtt tcatcaaacg agaaggccgt ccaagtacgg
    35161 aagcaacatt gactgcccgt ttgatcgacc gtccaattcg cccgatgttt gcagaaggct
    35221 tccgtaatga agtccaagta acgaacatcg tgatgagtgt agaaacagac tgctcgccag
    35281 ctatggcagc aatgctaggt tcttctttag cactatcaat ctcagatatc ccatttgacg
    35341 gcccaatcgc aggcgtagaa gttggtcgtg taaatggcga gtacgtcttg aatccaacag
    35401 tagaacaagc agaacaaaca gatatcgaat tgactgttgc cggaacgaaa caagccatca
    35461 atatggttga aagcggcgcg aaagaagttt cagaagaaga catgcttggt gcattgctgt
    35521 ttggttttga cgctatcaaa gagttagttg ctttccaaga agaaatcgtt caagcagtag
    35581 ggaaagaaaa aatggaagtg actttactgc aagttgatga agtattgaaa aaagaaatct
    35641 ttgatgcttc ttacgctaca atgaaagcag cagtaatgac tgaagaaaaa cttgctcgcg
    35701 aagataatat cgagcaggtg aagatcgata tccgcgaagc ttatgctgag aaatttgcag
    35761 gtcatgaaga tgaagatcac ttgttgaaag aagttaaaca aatcacagaa gatcttgaaa
    35821 aagatgtcgt acgtgaattg atcaccatcg ataagatccg tcctgacggg cgtaaattgg
    35881 atgaaatccg tccattgtca tcagaggtca gcttattgcc acgtgtacat ggatcaggtt
    35941 tgttcacacg aggacaaaca caagctttat cagcttgtac actcgcacct ctaggagagc
    36001 accaaatcat tgatggttta ggggtggaag tcagcaaacg attcatccac cactacaatt
    36061 tccctcaatt ttctgttggt tctacaggac gtgcaggctc tccagggcgt cgggaaatcg
    36121 gacatggcgc attaggtgaa cgtgcgttag cacaagtaat cccttcagaa gaagaatttc
    36181 cttatacgat ccgtttagta gcagaagtat tggaatcaaa tggttcttct tcccaagcaa
    36241 gtatctgtgc tggcacatta gcattgatgg atgcaggtgt accaatcaaa gcgccagtcg
    36301 ccggtattgc gatgggactt gtttctgatg gtgaaaacta tacgatccta actgatatcc
    36361 aagggctgga agatcatctt ggcgatatgg actttaaagt tgccggtaca aaagatggta
    36421 tcactgcttt acagatggat atcaagattc aaggaatcac agaacaaatc ctaactgaag
    36481 cattaactca agcaaaacaa gcacgtatgg aaatcttaga agaattgact tcaacgattg
    36541 ctgctccacg tgaagaatta agccaatatg caccaaaaat cgaaatgatc cagatcgaac
    36601 ctgctaaaat caaagatgtt atcggtaaag gcggcgatac gatcaacgga atcatcgatg
    36661 aaactggtgt gaaaatcgat atcgatcaag atggaaaagt aagtattgct tcagctgatg
    36721 cagaaatgat caaaaaagct atcaagatca tcgaagattt gaccaaagaa gtcaaagttg
    36781 gtgaagttta tctagggaaa gttgtccgta tcgaaaaatt cggtgcattc gtcaacttga
    36841 tcaaaggaaa agatggactt gtccatattt cccaattagc aaacgatcgt gtcaataaag
    36901 tcgaagatgt tgttaaacta ggtgatgaga tccttgtgaa agtaactgaa atcgataaac
    36961 aaggacgcgt aaatctgtca agaaaagcaa tgttgaacga agatggttca acgaaaaatg
    37021 aagaaaaata atttaattca taggaataat gtacttgtga tcgtgaaggt gacaggggga
    37081 caataattat tcgaaaaaag gggactgtga caaaagccgt gtcacagtcc ttttttttcg
    37141 aataaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt taaaaacatt
    37201 aggagctgtt tttatttggc tgttcttatt acttgatgca gaacagcttt tttacaacct
    37261 ctccaataaa cgatgttcaa tcagctgtcc ctcgatatta gctcaaaaaa cggcaaaata
    37321 tgaggaacta tttttccgtt tgtttctcta atactcaggt caaaccgctg atttcacaac
    37381 ctctgattca cggtgttcca gaagcagctt ctacggaaat aagctgaaca cttttgttac
    37441 aacttctttt tttctggagt ttcttggcgc atatttttgg agtccttctg aataaagcgt
    37501 aaaataaaag tgagaaatat ggataaagga gggaaaggag atgctgaatt ttttatggtc
    37561 gttatttatc ggcggtatat taggtttgat agctggcgca attttaggaa aggaagtccc
    37621 gggaggcgta tctggaaata tcattatcgg ttttttaggt agttggctcg gcgaattttt
    37681 actaggacca ctgggaccgg aactcggcgg tttttatttg ataccagcat tattgggtgc
    37741 aattttttgc cttgctcttt attcatttgt ggcaagacgt atcagagaac gtccaaagaa
    37801 agaataattt tattcaaata agttgacact tcagtgtgtt acttggataa ttagaagcaa
    37861 tttaggaaag cgggtgtcat ggataagaat atcatctatc attcaagctt acctaaagga
    37921 tatccatata acaagaaatg agggaaaaac gtggcaacaa cgcacaatac caaaaagata
    37981 tcaatgactg gcctgcttgt agcgatggga gtcgtctacg gagacatcgg gacaagtccg
    38041 ctttacgtta tgaaatcgat tgttgaagat aatggcggat tgtcaggaat caatcctgat
    38101 tttgttatcg gatcagtctc attgattttt tggacattga ctctactgac aacaatcaaa
    38161 tatgtcgtga ttgcattgaa tgcagataac catggtgaag gtggaatttt ctcactctac
    38221 acacttgtcc gaaaaggtgg gaaatatttg atcattcctg caatgatcgg aggagctgcc
    38281 ttactagcag atggggtatt gacccctgct gtgacggtaa cgactgcgat tgaaggcttg
    38341 cgaggaattc cgcagttttt tgaccggttc ggtaataacc agaacgtgat cgtcattatc
    38401 actttagtta ttatttttgt tttattcatg gtccaacgct ttggtacaga tattgtcgga
    38461 aaagcattcg gaccaatcat gttcgcttgg tttacttttt taggcgtaat gggattaatg
    38521 aacttcagtc aagactggac agttatccgt gcactgaatc cttattatgc tatccagctg
    38581 ctttttagtg cagataacaa attaggcttg ttcattcttg gaaatatttt cctggctacg
    38641 acaggtgcag aggcgcttta ttccgatctt ggacatgtcg gcaaacgaaa tattcgtctg
    38701 agctggcctt acgtcaaaat ttgtttggtt atcaattatt ttgggcaggc tgcgtggttg
    38761 ttgaatgttt atcaaaaccc tgaagcacag caaatcaaga acctgaatcc attcttccaa
    38821 atgatgcctc aaagttggat cgtgatcggc gttgcttttg caacagtcgc tgcagtcatt
    38881 gcttcgcaag cattgattac aggttcgttt acgctagttt cagaagcaat caagttgaaa
    38941 ctgctgcctc ggttaaagat catgtatcct gggaacagta tcgggcaaat gtatatccca
    39001 gcagtcaatc tgattttatg gatcgcctgt tctctagtgg ttgtcacatt ccgaacgtct
    39061 catcatatgg aagctgctta cggtctatcg atcactgtga cgatgttgat gacaacagca
    39121 ctgctttact tttatcttat ccaaaatggg cattctcgtt ggcttgcata tttagttacc
    39181 tttttcttcg gtgcgataga agtcgttttc tttatctcaa gtgttgtcaa attcttccga
    39241 ggcggttttg tagccgttct gattggtttg atcatcttag tcgtgatgtt tatttgggaa
    39301 caagggaaca ttattcgcga atcgtcaacc gaggatgtgt ctttgagaga ttatatcccg
    39361 cagcttagag aattacgtga tgatacttct ttaccaatct atcagacaaa tgtggtcttc
    39421 ttggttcctg atatggctga agataaagtt ggccgacagt tcatctattc gattttagat
    39481 aaacggccta aaagagcacg agtctattgg tttgttcatg tggaagtgac ggatgagcca
    39541 tatacgaaag aatatcaagt agatatgatg gacactgatt ttattgtcca agtgaatctg
    39601 ttcttaggtt tcagggtcca acaggaaatc aacgtatatg ttcgccaagt catccatgat
    39661 ttgatgaaac aaggccgttt gccaaaacag ccacaaagat actctctgac accagggcga
    39721 gaagttggcg atttccaatt tgtcctgatt gatgaagtag tttccaatgt gacaactcta
    39781 ggaaaatggc aacgtcaaat catgcaggca aaactggcaa tcaaaaaaat cgctacctcg
    39841 ccagaaacat ggtttgggct agagtacagc gaagtaaaac atgaaactgt tccgcttatc
    39901 attggaaata ctcggaaaac atggttgaaa gagcggttgt gaaagtagcg ctttgacctg
    39961 aactaatgtc gaatgtcagc tacttgaaca ccgtttatca gaggtcatga aaaagctgta
    40021 ctgcaacaag cagtaagaac gagcagataa aaacagcttc tcatgttttt catctgtttg
    40081 ggcttaatgc cgtagatgca agcttttgaa taccgttttt atcagaggtc atgaaaaagc
    40141 tgtttagcaa caagtaacgc aatgattttg aagtgataag gagatgtggc tgaaaaatgc
    40201 cgcatctcct tttttattcc taaatatgaa tacgcattat tttcaatctt tttgtagaaa
    40261 aagggtacaa tagaaaaaga gaaaggcggg agaatatggt tatcaatttc attattttat
    40321 ttttgatgat tgcaattact tcattttttg tagcaagtga atttgcttta gtgaaaatca
    40381 gacgatcaag attggaacaa ctagaaaagg aaaatgtaaa gaacgccaag ctagctcttc
    40441 atgttacaca ccatttagat aactatttat cagctagtca attaggaatt acgctaaccg
    40501 gtttgatcat cgggtgggtc ggtgaaggat ctgtcgcggc tttactagaa ccagtgatcg
    40561 gtaaactacc ttttagttcg gctattagca gtacaatctc tgtggcgctg ggatttattt
    40621 tagttacgta tatcgatgtg gttattggcg aactgcttcc taagagctat agtatcgtca
    40681 atacggagaa ggtcgtcttg ttcgtagtta aaccacttca ttatttttat aaagtaatgt
    40741 ttccttttat ttgggtattg aatcattcag ctgcagggct agggaagcta ttaggtgtgc
    40801 gtctcgtatc tgaaggagaa gagacattgt ctcaagaaga gctgacatta gtcgcattga
    40861 attcttatga aaaaggagag ttgaccaaag aagaatatta ttatttggaa aacgtgtttg
    40921 aatttgatga taccttggca aaagatatac aagtagaccg tacgtccatg caagtattcg
    40981 aagcttcaga aaccgtcaaa gaagcgataa aagcttcgat tgaacaagga catactcgct
    41041 atccggttat cttagaatca aaagacaatg ttttaggtta tgtcaccttg ccagatttga
    41101 tcaagcaatc ttataaagat gaccagctga cagtagaaca gttgattgaa gagccgatcg
    41161 taacaactga aacgatccct ataaaaaaat tattgacgat catgcgaaaa aagggtaagc
    41221 atattgctat cttaaaagat gaatacggag ggacaagcgg cttagtgacg attgaagata
    41281 ttttagaaga aatcgtcggg gaaatccgag atgagacaga tttggacgaa gcattgatag
    41341 cggaacagtc agatggctct tatatcatct ccggcaaact aacattagat gatttccagc
    41401 gttattttca tgtagagatt ccagagtttg aagaaacgaa ttttactaca cttgcgggct
    41461 ttgcttctag ccgatataaa gaaataaaag caggaacaat cattgagatt gcatcattcc
    41521 gtttcacggt attagaatac cagcatgcac atatcgatta tttcaaagta gagtccacgg
    41581 aaagaaaaac agaataaaag aaaaaaggct gtgacatttt ttgtcacagc ctttttgtta
    41641 tctaaaaaac ctgttttcgg taaacgaaga agatattttc aataaaagtt ttcattttta
    41701 gccgcaacga tttcttttaa tcaaggctat cttgcttctg catgttctaa tgtgaaatgt
    41761 tcctcttcac ccatgtgttt caattgtttc atcgaacgca tataagagta aagcaacacg
    41821 gcaacagaac cgatccaagc taaaggagaa gctgctgctg ctcctgggta tccccacatt
    41881 cctgtaagaa cgatagctgc aaaagaacgc ataagcaatt ccatcatacc ggcgatcgta
    41941 gggatcttgc tttgtccaag cccttgaagt gtgtagcgta aaatgaacag gactgctaag
    42001 atccagtaaa gagacccatt gatattgaag taggtttgtg ctaattcaaa tacacgtgtt
    42061 tctgatgggt taacaaacaa ttgaacaaag aaatgaccga agaaaatgac gatagcacct
    42121 gctaagagac taaatccgac actcatcagt aaagtttgac gtaccccttg caagattcgg
    42181 ccatattctt tcgcaccata gttttgtgca gtgaaagttg ccattgcaat accaaatgac
    42241 atcatcggct gatttgcaaa ttggtcgata cgaccagcag cggcttgagc agctacgaca
    42301 tctgtcccta gactatttag tgctgcttgt aacacgatag caccaacagc gatgatcgaa
    42361 gattgaaacg ccattggcaa agcagcattt aggtgtacag caaactcttc tttttcaaat
    42421 ttgaaatctt ttttgcgtaa ctgtaacaaa ggcatttttt tcttgatata cactagacac
    42481 agtacgctgg aagaaacttg tgcgattact gtagcaatag ctgcaccttc cacacccatg
    42541 ccaaaataga taataaagat caaatcaagt acgacgttga tgatgaccgc aatgatcaga
    42601 aagaatagtg gtgtgcggct gtcaccaagt gcgcgaatga cattagaaag caaattgaaa
    42661 ctgactgatg caaagatccc taataaaata attgaaataa atgtttgtgc ttgatcaagt
    42721 atttcaggag gagtttgcat caattgaagc atcggacgaa taaacaggag acttaggaca
    42781 gttaaaatca cagtgacgac aagactaatg acaacactag cagcaaaact tttcttcaga
    42841 ccccgataat cttttgcacc gtaacgttgt gcggtaataa tggctaaacc agctgttaaa
    42901 ccttgggcaa agccaatgat caaaaatgtc aggcttccag ttgcaccaac cgctgccagc
    42961 gcatttttac ctagcgtctg acccacgatg atcatatcga tcatattgta gaactgctga
    43021 aaaacatttc cgatcagtaa aggaacagtg aacatcaaaa tcagtttagc ggggcttcct
    43081 tttgttaaat cgcgcatctt ttaaactcct tatatcttta aaatttagtt agtagtatat
    43141 agcgtttatc ctgagaatac caattagaaa tcaatgataa tttatgaaac tccataaaaa
    43201 gaagccaaga attgagtcaa agaatcaaca acttggcctg gtctgtaaaa taagggacat
    43261 ccacgtttat ctatcacctt aatattgtct aatctcactt ggtaaaagga tcgtttctct
    43321 cgtaccgtca tcataggcgt atacttgagc aggaaattgg ttcgaatagg caaacggcaa
    43381 atcaatatcc atttccacat tgcttcctgc ttgagaaaaa tgcatcgtca cttttccacg
    43441 attttcgatc aaatcaaagg ttaatatatt ttctaatgga atgactccct taagatctag
    43501 gtcaataata aaccatatac tgtcaatcaa ttcatctggc aaacttgata cgacaccaac
    43561 tgaagcgtaa cggttgcggc tgctatcaaa actttcaaac atctgtttaa cctccttcga
    43621 atcaacgaat gttttatttt tataattaat cctaggataa actagaaata cttacttcaa
    43681 cgagggttca ttctaataaa gaatcaggaa aaatgcaaca ataattgaat actttttttc
    43741 aaagtataaa tttttataaa ggtacgataa aagtatgcga ggaaaagctt ggtaaattag
    43801 gatttttcgt ttgtaaagcc atttacaaaa gtggaagccc catatgaaaa taaacgatta
    43861 gaaataaaaa aggcgtgtta aaagctgctg acccgccgct tctaacacca cctgattgtt
    43921 tcagtattat tcttctgttg aaccgatcac ttctggttcg ccaccttcac ctgtgctttc
    43981 ttcttcttca ctttgagcga ctacagcagc gatttgctct tcactgtcgg tcacgaattt
    44041 gtaatcgtcg ttttttggaa gatcggctac tgtaatagaa tcacctaatt ccaagtttgt
    44101 cacgtcaact tcaattcgtt caggcaattt gtctggtgta gcagatacga ctactgtata
    44161 caagttttga gctaacactc caccagattt agcacccact gattcgccaa caagaacgac
    44221 ttctgtttcg acttctgttt cttccgacat gtttacagat agaaattctg catgttcgaa
    44281 atgattggta aaagtgttcg tttggatctt gtgaaccaat gtattgactt tttgtccatc
    44341 gatgtccatc gtgatgactg tgttcgctcc atgttcacgc aacaatttgc ttagtgtcaa
    44401 accatcgaat gagatagggg tactttctac tttataccca taaacaacag ctggaacttt
    44461 cccttcgtga cgaagtttgt ttcttaatga acggggacgt acttctcttt tacttacttc
    44521 taatgatact gacataacca aaattcctcc ttaaaatgtg actgtctgct ttggtaacgg
    44581 acagatttta tttaagtgaa ttctaaaggc taatctcgtg cacatcatcg aaggcagggc
    44641 gcttaaaaaa gcgcaaaagg agagatgcgc gcatctcccc ttagaaagtc aggattcaac
    44701 ttttctccac caggtcatta cgctgtgtat gagaaatcca attcacttta ctatttaacc
    44761 gtaacgtgaa ctgttaatta agtcaaggaa taactatgat gatgtatttg caagcaagaa
    44821 tattcaaggt ataattaatt agtagtaata agaaaggaaa cagcatgcgt ttagataaat
    44881 taatagaaga aaaattagaa acgacacgca aggaaatgaa gcggctgttt gctttgaagc
    44941 aagtgatgat agatggcaca gtcgaatttc gacaaaatcg aaatgtagat agtttactgc
    45001 atcaaatcga agtagcaggt gaacacttag aaacaaatga gtcctattat cttctcaata
    45061 aacctgaaaa agctgttaca gctgtatcag atccagaaaa gacgacagta atcgatctga
    45121 ttgcaagtgc cgatcgtttc caaccattat atccagtggg aagactagat agagatacaa
    45181 caggactatt gctgatcact tcgaatggac aattgggata tgaaatgcta cttccagaaa
    45241 aaaaggtttc aaagacttat caagtagttg tgaacgaaag ggtgacaccg gcagacgtaa
    45301 cagcatttga aaatggaatc gtattcgctg atgggtatca atgtaagccg gctgatttaa
    45361 aaatactcaa agcagacaag caccaaagta cggttttatt gacgatccaa gaaggaaaat
    45421 tccatcaagt caaaaagatg ttcttagccc aaggaaagaa agtgaccgca ttgaaacggc
    45481 taagcatggg accattggaa ttagatgaac agctaggagt aggcgagtat cgatcgttga
    45541 cattagcaga gatgaaaaag ttaagtattt attttaaata ggaggtcaaa aagatgaaat
    45601 ataaaacttt attgtttgat gtagatgata cattgttaga ttttcaatta actgaagcca
    45661 atgctttaag tgctttgttt gcagaacaag gaatcacatt gacaccagaa atcgaaacca
    45721 gctataaggc tttgaatcat catttatggc aggagtttga aaaaggcaga ctatcacgag
    45781 aagaaataat aggcagtcgt tttgggttac ttttccagca atttgggaaa acagtagata
    45841 gtgatcaaat ggaaaaacgc taccgccact acttgaacca aggtcatgat cttgttccag
    45901 gaagtctaga aatcctgaaa aaggtccatc cgcatgctga tctttatatt gtgacgaacg
    45961 gtgtgtcgca cacgcaacat cggcgattaa cagatgcagg aatgcttgat tacttcaagg
    46021 atatctttgt ctcagaagct gtcggagcgc aaaagccgat gaaagaattt tttgatcatg
    46081 tcttttccca cattcctaat tttgataaaa agcaaactgt gatcattggt gattctttga
    46141 cttctgatat caaaggagga aatatggcag ggatcgatac gatttggttc aatagaaata
    46201 aattaccaga aatcgaagaa atccagccta cttatcgaat cgattcatta gaggaacttt
    46261 attcattatt ggaaattgaa aaggattaat tttcagaaaa atatagcatt ttcgtgtttt
    46321 ttcgctataa tagattagaa tgaaaaaacg gatacgaaag aggttttagt caatgacaaa
    46381 aagcaagcca attatcattg gtgtgacggg tggatccgga agcggaaaga caagtgtcag
    46441 ccgtgcgatt ttcaacaact tcccggacca ttccatcatg atgcttgaac aagattctta
    46501 ttacaaggat cagagccatt tgagttttga agaacgattg aacacaaatt acgatcaccc
    46561 atttgccttt gataatgatc tgctgattca gcatgtaggc gacttactga actataaagc
    46621 cattgaaaaa cccgtgtatg actatgtagc gcatacaaga agtcaggcaa cgatcattca
    46681 ggaaccaaaa gaagtgatca ttctagaagg aatcctgatt ttagaagacg aacgtctgcg
    46741 tgacttgatg gatatcaaag tatacgtaga tacagatgat gacattcgta tcattcgtcg
    46801 tatcaaacgg gatatggaag aacggggacg cacattggac tccgtgatcg aacaatattt
    46861 gacagttgtt aaaccgatgt atcatcaatt tatcgaacca acgaagcgct atgcggatat
    46921 cattgttcca gaaggcggag aaaaccacgt tgctattgat ttgattacaa caaaagtagc
    46981 tagtttctta aaccataaat agtgcgcagc gaaacagttg aacggactgc gaccgtttga
    47041 aaagcaagga ttctgctttt tgaacagtca ccagccaact gtttttttat ttataacaag
    47101 aggttgtgaa aacagcatct tgacctaagt attaaaaaga agaaacggaa aatagctgtt
    47161 tatattttac cgttttttga gctaataccg aaggtcagct gattgagcac cgtgaagtag
    47221 aggttgtaaa aaagctgtct ttgcatcaag cgataagagc atccaaataa aacagctcct
    47281 cacgtttttc gttgggttgg gcttattatc gtagatgcaa gcttttgaac accgtttaac
    47341 gagaggttgt aacagaagtg gtcagatgca ataaataaga agaaacttac gaaaattatt
    47401 tctcgaattt gtgtgagatt tcgacttatt tttttgaaga agctcttttg tcgcaccccc
//
LOCUS       DAHUVV010000013        46205 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.013, whole genome shotgun sequence.
ACCESSION   DAHUVV010000013 DAHUVV010000000
VERSION     DAHUVV010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.013"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..132)
                     /locus_tag="NK767_000711"
     CDS             complement(<1..132)
                     /locus_tag="NK767_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCH6693725.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..781
                     /locus_tag="NK767_000712"
     CDS             542..781
                     /locus_tag="NK767_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693726.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            848..1057
                     /locus_tag="NK767_000713"
     CDS             848..1057
                     /locus_tag="NK767_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693727.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            1117..1788
                     /locus_tag="NK767_000714"
     CDS             1117..1788
                     /locus_tag="NK767_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCH6693728.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1841..3817
                     /locus_tag="NK767_000715"
     CDS             1841..3817
                     /locus_tag="NK767_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCH6693729.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3830..4585
                     /locus_tag="NK767_000716"
     CDS             3830..4585
                     /locus_tag="NK767_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCH6693730.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4582..5406
                     /locus_tag="NK767_000717"
     CDS             4582..5406
                     /locus_tag="NK767_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693731.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5707..7806
                     /locus_tag="NK767_000718"
     CDS             5707..7806
                     /locus_tag="NK767_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCH6693732.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7844..9901)
                     /locus_tag="NK767_000719"
     CDS             complement(7844..9901)
                     /locus_tag="NK767_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HCH6693733.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(10015..10227)
                     /locus_tag="NK767_000720"
     CDS             complement(10015..10227)
                     /locus_tag="NK767_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693734.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            10414..10881
                     /locus_tag="NK767_000721"
     CDS             10414..10881
                     /locus_tag="NK767_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693735.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10878..11120
                     /locus_tag="NK767_000722"
     CDS             10878..11120
                     /locus_tag="NK767_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HCH6693736.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            11138..11440
                     /locus_tag="NK767_000723"
     CDS             11138..11440
                     /locus_tag="NK767_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693737.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            11512..13566
                     /locus_tag="NK767_000724"
     CDS             11512..13566
                     /locus_tag="NK767_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693738.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELGQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            13566..16178
                     /locus_tag="NK767_000725"
     CDS             13566..16178
                     /locus_tag="NK767_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCH6693739.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            16175..18706
                     /locus_tag="NK767_000726"
     CDS             16175..18706
                     /locus_tag="NK767_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693740.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18758..19090
                     /locus_tag="NK767_000727"
     CDS             18758..19090
                     /locus_tag="NK767_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693741.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            19091..19684
                     /locus_tag="NK767_000728"
     CDS             19091..19684
                     /locus_tag="NK767_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693742.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19847..21541
                     /locus_tag="NK767_000729"
     CDS             19847..21541
                     /locus_tag="NK767_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCH6693743.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            22229..24106
                     /gene="ltrA"
                     /locus_tag="NK767_000730"
     CDS             22229..24106
                     /gene="ltrA"
                     /locus_tag="NK767_000730"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCH6693744.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            24237..24614
                     /locus_tag="NK767_000731"
                     /pseudo
     CDS             24237..24614
                     /locus_tag="NK767_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24614..25819
                     /locus_tag="NK767_000732"
     CDS             24614..25819
                     /locus_tag="NK767_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCH6693745.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25835..26479
                     /locus_tag="NK767_000733"
     CDS             25835..26479
                     /locus_tag="NK767_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693746.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            26490..27296
                     /locus_tag="NK767_000734"
     CDS             26490..27296
                     /locus_tag="NK767_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693747.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            27318..27548
                     /locus_tag="NK767_000735"
     CDS             27318..27548
                     /locus_tag="NK767_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693748.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            28094..28369
                     /locus_tag="NK767_000736"
     CDS             28094..28369
                     /locus_tag="NK767_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693749.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            28411..28833
                     /locus_tag="NK767_000737"
     CDS             28411..28833
                     /locus_tag="NK767_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693750.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28852..30444
                     /locus_tag="NK767_000738"
     CDS             28852..30444
                     /locus_tag="NK767_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693751.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            30456..30791
                     /locus_tag="NK767_000739"
     CDS             30456..30791
                     /locus_tag="NK767_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693752.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30938..31132
                     /locus_tag="NK767_000740"
     CDS             30938..31132
                     /locus_tag="NK767_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693753.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            31164..33536
                     /locus_tag="NK767_000741"
     CDS             31164..33536
                     /locus_tag="NK767_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693754.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            33597..35057
                     /locus_tag="NK767_000742"
     CDS             33597..35057
                     /locus_tag="NK767_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCH6693755.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            35068..37272
                     /gene="topB"
                     /locus_tag="NK767_000743"
     CDS             35068..37272
                     /gene="topB"
                     /locus_tag="NK767_000743"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCH6693756.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            37288..37437
                     /locus_tag="NK767_000744"
     CDS             37288..37437
                     /locus_tag="NK767_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693757.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            37511..38104
                     /locus_tag="NK767_000745"
     CDS             37511..38104
                     /locus_tag="NK767_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCH6693758.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            38120..39019
                     /locus_tag="NK767_000746"
     CDS             38120..39019
                     /locus_tag="NK767_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCH6693759.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            39022..39507
                     /gene="ssb"
                     /locus_tag="NK767_000747"
     CDS             39022..39507
                     /gene="ssb"
                     /locus_tag="NK767_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCH6693760.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            39873..40133
                     /locus_tag="NK767_000748"
     CDS             39873..40133
                     /locus_tag="NK767_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693761.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            40574..40780
                     /locus_tag="NK767_000749"
     CDS             40574..40780
                     /locus_tag="NK767_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCH6693762.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            40780..41031
                     /locus_tag="NK767_000750"
     CDS             40780..41031
                     /locus_tag="NK767_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693763.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            41046..41483
                     /locus_tag="NK767_000751"
     CDS             41046..41483
                     /locus_tag="NK767_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693764.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            41476..42183
                     /locus_tag="NK767_000752"
     CDS             41476..42183
                     /locus_tag="NK767_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693765.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            42564..42743
                     /locus_tag="NK767_000753"
     CDS             42564..42743
                     /locus_tag="NK767_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693766.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            43084..43347
                     /locus_tag="NK767_000754"
     CDS             43084..43347
                     /locus_tag="NK767_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HCH6693767.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            43341..43691
                     /locus_tag="NK767_000755"
     CDS             43341..43691
                     /locus_tag="NK767_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCH6693768.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(43715..44329)
                     /locus_tag="NK767_000756"
     CDS             complement(43715..44329)
                     /locus_tag="NK767_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCH6693769.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            44643..45065
                     /locus_tag="NK767_000757"
     CDS             44643..45065
                     /locus_tag="NK767_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693770.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            45075..45278
                     /locus_tag="NK767_000758"
     CDS             45075..45278
                     /locus_tag="NK767_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693771.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            45489..46073
                     /locus_tag="NK767_000759"
     CDS             45489..46073
                     /locus_tag="NK767_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCH6693772.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT        16943 a         7305 c         9364 g        12593 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaatagta
      541 gatggcgcga ccagttttag aggcgatgga tatggatact ttgacaaaag gaaggtatgt
      601 acagctgtta gctcgtggtt attcacctaa agaagtattc aaagccttat ctaaaggaac
      661 agatatggaa aaagaaagat tgcgaaaaga attcgattat tggagaaccc ataatggaat
      721 caaggatttg aaaccagcac ggccagtaat taaacgaaag aaaaagataa cccaagactg
      781 aagtgtagag cgtatgtgat cataggcttt tttatttttg caaaaaacaa ggaaggtagg
      841 aaaactcatg aattatgaag aaatcgaaaa cagaaaaaaa gtgtcgaaag aaatggaaga
      901 aaagttacta aaaacgatga agcaaaaaca ccttaaaaga ttatctgtcg cgcaatatat
      961 caatgatatg cagttaacag gaaaagagaa agcttgtttg ttaggttcaa tgaaaaactt
     1021 tgaacagttg agacgaactt atagaattca tttttaaaac tttttctatg gaaatgagaa
     1081 aaagtttttt tgttttgtct gaaaggaggc agatggatga aaaaatggtt atttggtttt
     1141 ttaggagtca ctttaatcgt cttttgcagt gtgtttggct atgttagcta ccaaaaacat
     1201 gaaggagagg tgtttaagca aaatatagaa aaaaagatgc ccgtagatca aatcaatgca
     1261 catgcaaaga gttataagga ggatgcaacg aacgtcaata atgatatgtc tttggggcaa
     1321 atgttgtcca tacaaaaaga agcaatcgaa atgggcgtca ataaacaagt atttgcacaa
     1381 attcagattc ctgcacttgg tttagcattg ccaattttta aaggggcaaa tcaatacact
     1441 ttgagtcttg gagcggctac ttatttttat gaagatgccg aaatgggcaa aggaaactat
     1501 gtcctagcag gtcataacat ggaaatgcca ggtgttttat tttctgatat tcaaaagttg
     1561 tcattagggg aggtgatgga cttagttagt aacgatggcg tctatcgtta caaggtaacg
     1621 cgtaaattta tcgtacctgg gtactttaaa ttaatcgatg gcgttcctga agagaactct
     1681 ttcttgtctt tgccaaagaa aggagagaaa cccttattaa cgttgtttac ttgtgtttat
     1741 acgtcacaag gaaaagaaag atacgtggta cagggagagc tacaataaca aggttttaat
     1801 caacgcgagt cgttgaaata tgaaatgagg agagtaaaaa atggcaataa atcggtatgt
     1861 aaaaaaagct ggaatgcttg ttggaatgtt aggaatcatt gcaggctgct ttcaggggta
     1921 tcaagtacat gcagaagatg tcacgcaaaa aacaccacca gagaaggtca atatcacggt
     1981 tcataaatta atgtacgatc aaggaacgca attgaatgtt gatattgacg ggatcaaaaa
     2041 cgatggttat acgcacgatc aatatcctac aggtgtaacg aaatacaaca aggcagatta
     2101 tggtgatgtt gagtttacgt taggaaatat tacggatcaa gtattaccga gagaagattc
     2161 tgatctaacc aacgcgaaag tggacgagat tgtacaagat gtagaagaca agggatctaa
     2221 cagtgagtac gtcaagaatg caaccaacaa aattacttca gctgtagatg aaaatggtga
     2281 gatcactttt actgatcaac cagcctatgt caattccaaa gggaatgtat atgtggttta
     2341 tgaaagtaaa tccgctgctg gattagtcac acaaaaagca aaaccaatgg ttgtgattgc
     2401 gccaatgaca gataaaacag gatcaggatt tttaaaaaat attcatttgt atcctaagaa
     2461 cattgtttct aagttgagtt ttgaattaac gaaatttggt gacgatggcg cagaccaaag
     2521 caaacaaacc cttttaaaag gggcaaaatt tgagttatac aagggcgaac ctggaaaagg
     2581 aacaaaattg ggtgatcttg tatcggatga tcaaggaaag ctgactgcga ctgatttgac
     2641 gttaggaaaa tactattttg tggaagttcc ttcggaagtt gtagtgggat cagatcaaga
     2701 accaacagca gatcaatatt tattaggcgc agacgctcgc aacgatgcgc acaataaatt
     2761 aacctttgaa attacgaatg atggcgtgac aagtgactta aaagcttcat acgtaaacta
     2821 taaagcacca gtgatcgata aaacagtaac aaatggaaca ggacaagaac attctttcca
     2881 aattggtgac gcagtaaatt atcaaggaac gattcatatt ccaacagaca ttgcaggtgg
     2941 cgcagacggt atcacagtaa acggtgtgaa aagcgaaact tctccttaca gtgtatttaa
     3001 atggggagat acagcaggta aaggactttc ttatgttgct gaaaaagcag atattaaagt
     3061 aacaaataat gatggttcag tcgtattaaa agaaaatact gactataaaa tcgaaaattc
     3121 tgatagtggt tttgtgatcg attttatcgt aaataatgga caagtaagtg atacagttgc
     3181 gaatttacat ggtcaagatc taaaaatgac ttataacatg tttgtgaatg atagtgctga
     3241 aatcgcaaat ccattaacaa atagcgtgga tttcgtttat aacaataatc catttaatca
     3301 ggaagaacac cacgaaaaaa cgaaagctga cgttgtgact tatggcgcaa aattcttaaa
     3361 agtcgatagc ggcctatttg gaacaggtat caaagcaaca ccgcttgaag gcgcagaatt
     3421 tgcagctaag aatgcagaag gtaagtacta cggtggactt actgatacag ataaagatgg
     3481 tgtcaaagaa gcagtttggg tagacgatgt agcaaatgca gcaatcctaa aatcagataa
     3541 agagggtcat tttgaaatca ctggtttaac tgaaggagat tactcactgg aagaaacaaa
     3601 agctcctgaa aactatcaaa aattaacaaa agagatctct ttcaaagtag acaaagattc
     3661 atacaaagaa gaaaaccgaa tcacgatcaa aaataatcaa aaagcttccg taccaatgac
     3721 tggtagcaac ggtttccaat cttacgtatt gatctcttgc ttgttgctag gagcaggggg
     3781 actttctgca gttgtttact ttaagaagaa agcctagaag gatcagctaa tgattattcg
     3841 taagccccca aaaagaagag tcatgcacaa actgatgata tttttttggt tgctgtcggc
     3901 agtagcagtc tatttaattc cagttgtgga aacaaatgta gcgaaatttc aacaacagca
     3961 aagaatttta gccattgaaa atcttgaaaa aacagattct tctttggatg ttgcgaaaat
     4021 agagttaggc gatcctgttg gtgttcttac aattccaagt atttctttaa aactaccaat
     4081 ttatgacgga acttccgaca aaattttaga gaatggtgta gggattacgg aaggaactgg
     4141 cgatattaca ggaggaaacg gtaaaaatcc tttaatcgcg gggcacagtg gactttataa
     4201 agataatctt ttcgatgatt tgccttcagt aaaaaaaggg caaaaattct ttattaaggt
     4261 aaatgaagaa caacatgcct atcaaattta tcgaattgaa gaagttaaag cagaagaatt
     4321 acaaaaaaat tatttagatt atcttatgcc tgaaaaagac caagatagga tcacgttaat
     4381 gacatgcaca ccaaaaggtg tcaactcaca ccgcttttta gtgtatggtg aacgagtttc
     4441 cttcagtaaa gaagatttaa aactacagaa aaaagcaaaa aataggaaat caatttcatt
     4501 ggattggcga ttagcactgc tattcgcttt tttttgcgtt cctattattt ttatttataa
     4561 aaaaataagg aggagatcgg aatgaataag agaaaatggc tgtttgtttg tgtagcagct
     4621 ctcttttcta ctgtagctta ttcgcaactg tctttcgctg acagcagtga tagtagtacc
     4681 actcaaacaa tagataattc aattactgca ccaagcgaaa cggacagttc ggagaatact
     4741 acagaagaaa cggcagattc aaatataagc tttactgaat caacgaatcc aacgtcagaa
     4801 agttcttcag agacttcaga aaagccgcaa gacgtagaag atacgcaagt gatcaagcaa
     4861 gccattcata ttcaaaaaat tatttcttcg aatcaaatca acaatcaagg cagtcaagta
     4921 gcccagcaag aaggtatcaa tggcgcgaag tttattgtct acgatatcac gaatatctta
     4981 gaagaaatgt cacaagatga taatgagaaa aatcaatctg tagaaataat tgagtcaaaa
     5041 ttgaaagatc gagcaaaaaa actttcatac gaccagttga aaaaagtggc tgaaggagaa
     5101 accaaaacga ttgatgatca ggaaggcgtg attgaatttt ccattgaagt acctgcaaac
     5161 aaaaaacaag catactatat cgttaatgaa tcaagccctg aaaacatttc aaacagtgaa
     5221 gatattgttt tattgacacc tgtttcaaat gaaaaaggtg aatttttgac ggatgtttgg
     5281 ttatacccta aatcagagaa atcagagccg aaagaagaaa ttaaaaaagt tgtgtcaact
     5341 ggtgtaaaga agaacttttt tgaaaattgt tgggaatttt ttgctcaatt attttttgat
     5401 aattaggatg aaatgatgaa aataataaga atattagttt ttaatctatc ctttttagcc
     5461 gcaacaatat tgtgttgcgg tttaattttt tctcaattat caatttatcc aagatatcaa
     5521 cgattgttca ttcattttct aaacattcaa tgtgcttcca tgccattaga ggtgattaaa
     5581 aagctcttga tcaatccaat ttttatggtt agtttggttc ttcttacagg attggtaacg
     5641 tgggtattag gaaaaaaaga atgaaaagct tacgatcgtt taaaaaaata ttagatggag
     5701 ggcattatgg aaaataaaat tcattgcact acgggaagag taattacttt agtgttgttt
     5761 ttgttccttc ttctttttgc gaggggaact gcagtccagg ccttagaaaa agttgatccg
     5821 agtcaaatca aagaaaataa taatctggtg ctttatcatg atagtgattc attgagaaat
     5881 atcgaagtaa ctgggatcaa tggatttaag atactagatg agtattcaac taaaaaacaa
     5941 aaagtacttg cttggccaaa agtcaaagat ttgaaaagta cgtcaaaaat cgttgcccat
     6001 tatcaaaaag taggtactta tctaggaaaa gaaattgatt gtgacgtgac tttttctaat
     6061 tttgtagcgc acgctggggt taaccaagac ggattagcta aaacaattaa tggcaaaaag
     6121 tattataatt atttgctctt tgataaaaat atttatcgag gatttttcat gcagtcaatc
     6181 aatgtcatga atgcaaaact agaatttaag tatgcaggaa caaaaacaaa agtagctttg
     6241 agtgaaccac agccagaaat tcaatcaaaa atgagatcga tgagagcagc tggtattgat
     6301 ttaaacgata catttctatc catttacagt ttaaatggac agtatcagca acgtgtgtct
     6361 tggtataatg acaataatga atctgtcggt gaacgtaata ttgatatgag agaatttgtt
     6421 gggtatgaaa aaatgggtag cttaccttat tttgtcacaa cagatacagc atgtgcagaa
     6481 tacaaagctc ctgcgttatc aacaaacaat ttaacttcaa aagtagtggg aggacgtgca
     6541 gaaaaggctt atagctcgaa tgatcatttc accgatgttg taggagctga tacttatcac
     6601 agaagtggtg taacgtatac gcttcaaggc gcttccccaa cattcatgat tggcgcaaat
     6661 acgaatagta tgatgtttag ctttgatact gcattgctat ggacaccaca accatcgaag
     6721 cctacaaaag aagtgtttaa caaagctaat actgaagagg cagcacacaa tattgacaaa
     6781 aaagtgattc cacaaggatc agatgtttac tatcatattc accaaaagtt tgatgcatta
     6841 acagtcaaca caatgaacaa atacaaatca tttaaaatca ctgatacctt tgacagcaaa
     6901 aattttgata tggtatcgga tgggaaaaac tatgatggcg cattgtggta tgttgaagcg
     6961 aatggtactt ggaaaaaagc tgatacagga ggaaagttaa ctgtatcagg taatacagtc
     7021 acttatactg cttctgacag ctttttagct aacgctgcaa attacggtag ggattatatt
     7081 ttgcagctcc atatgagagc aaaaaatgat gcaaaaggaa ttgcaccaaa tgtggctact
     7141 gtaacgttta acaatgatcc gaacaaagga acacaaaaaa caaatacagt agaaaactat
     7201 ttctctctta aaccaaataa aaaagtggaa caagatggga aagatgttga tggtcgaaat
     7261 gatggtaaaa aaggaacgcc tacagctcct ttaaatgcag gatctgaagt acaatatcta
     7321 gtcacacaaa aatggcatac aaaaggtgta gacattgcct cagatcatta caagcaattt
     7381 agtattcaag atccagtgga agatcgtttg acgtataaag aaggttctgc gcgagtgata
     7441 gatatgagta caggaaaaga tattacttca gaaggtacac taacctatga tagcaattca
     7501 agaacgctga agtgggaagc ttccgctgat ttcttaagta aaaatctttt agatggacga
     7561 gaaattcagc tgatatttac agctaaaact cctttgaatg aagaattgga tatcgacaac
     7621 caagcaaaag tttcagtaga aagtattgag aaagcaacta atgtagtaac gattggggta
     7681 aaacctaact tacctcagat cattgtgcca aaaactggat cgaatcattt agtgctaact
     7741 gttattggaa gtatattatt tcttacttta gcatcattgg gctatgtagt tttaaaactc
     7801 aaataaatat aaaaacaagg ctgagaatca gccttgtttt cttttatttg aagattggat
     7861 tcgcactaat aaatacatta tctacgccaa ttctaccagc agagtcaact acaaaattat
     7921 gacctaatcc gagaatactt tggttattat aaagtaaatt tgcattatgg gcagggctat
     7981 ttttccatcc taccatggca tcgatagcac catcgtctga taaaaggtca tcaatataca
     8041 gcccagtttg acttatgttt tctcccttag caccgtagca gcctaagctt tccaataaat
     8101 cttggaaagt accaccacca gggcgatagt gactgaagct gacttgtaat tctttagctc
     8161 gaatatctgc agcctgttga acaatatcat tgaattgcat tggggcaaca ccattttgtt
     8221 ggcgataatc atttaaccaa gcaagttctt tttgacgcat aaagttacgt aattcgtcta
     8281 atgaaatctt tttatatttg aatgtgattg ttttattttg atcgacgtta ttaatggatc
     8341 gttggccgtt tttatccacg gtaacggtag gatcgtcatt tctgcacaag aaataatttt
     8401 gatcagtcat atttttccaa ggagtagaga agctatcccc ttcgtatact gtttttgatt
     8461 cactagagag aacagcgttg gtatcagcat ttacatgatt aacagtcaca ttaaatttac
     8521 gagtgtagtt gaaaatataa ttttgacttt cagtcacatt gtttaaggtt actgaatttg
     8581 ttggttctac ccgatcgtct aaaacatatc cttgaataga aggagcagtt aaagtgacac
     8641 tttcaccgtg agtgacgttg attatctgtt ctttcaaaat atcaactcgc ccctcatttt
     8701 tgtagttgtt attgcacaac aaattgattt gatgcacttg attataattg aatgtgattg
     8761 ttgtattacc tgtgattgtt ttgctttgag tatcattacc aatcactttg tagctaaaat
     8821 tatctttagc accttcgcta tatttaaatg cttttgcctt agcagtgtaa gtagaaccag
     8881 aagtcacttt tgcttgatct gtagcaatca cttgacctgt ttgattgtca atatggttaa
     8941 tggtaaccat atattctttt ggcgtttcag gtttgtgttg caacccttta aatgcagcat
     9001 caagactatt tttagcattg tttacttgtg tttgagaaac cttagaatcg tctagtactt
     9061 ttttagcatt ggataaagca gtctgaaaat tattccaaga ttcttctgta aaatctcctt
     9121 tagcagttga ttttactcgg ttgtataaag cttgtaattc agctttattc acagtaggaa
     9181 ctggtttttt ctttaatcct ttgtaagcag aatctaattt atttttggca ctatccactt
     9241 gcttttgagt cgcttttgag tcatttaaca ctttcttagc attgtttaaa gcggtttgga
     9301 atgcgttcca ggtattgtcg gtatagtcac cttttttcgt tcccttaacg ctcgtataaa
     9361 gagcttctag agccgattta ttcactttgg gtttagaggt gtcagcgcca taccaaccga
     9421 taccttcgta tttccagcca tagtctttgg ttaaccattg tacttctttc caacttgcag
     9481 tgtagtgatg tgagccagat tttacaccag gaaggaaaag gcggtagatc ggtttattat
     9541 ttttttcggc agaacgccag gcaggccctt cataagtcca gccgtaattt ttagtcaacc
     9601 atttcacttc atcccaacta gctgtgtaat ggtgatcacg aagaccttta ttgtacaaac
     9661 gatagacaag atggccaccg ctagtcggtg cttcccaggc ggggccttca taatttcccc
     9721 attttatctt tactagatgg tcacgttctt ttttgctaga ggtgtaaaaa tgttcattat
     9781 tatttggatt gtaaaggcga tacatgacgt tagttgaagc agcgtcaaca gatccagcaa
     9841 gactaagtga ggcagcaaat gttccaatag ctaaaacagt ttttggatta aataatttca
     9901 ttttttcaac tcctaaattt aattaaaaat gtttacatga ttattatata tcggctaacc
     9961 gatatagtca ataaaaaata agaaaagcct tatataattt tggcttttct tattttattt
    10021 gttagttgaa gtattaatat caacgatttc ttcagccaat ttatttaatt tcatagcatt
    10081 tatgaatctc atgttgtcta tactagagcg tttgttggca aggtcactta ctgtagttaa
    10141 gggtacgcca gattctatgg atatagaacg aactgattta ccactatcaa acagcaacca
    10201 ttcgattttc tttttatcag cgtacatttc aatgctcctt cttgtttttt ctttattata
    10261 gctctttttt gtaatgaatc acaatatcaa gagaaaaatt ttctatagaa aggtggattt
    10321 ttatgagtat tcgttctccg tgtatccaaa tgaggtttgt tgctactttc gttatttgat
    10381 atactaaaaa taaacaacga aagaaggaat aaaatgaaga aaaagcaact atggtttgtc
    10441 tctatgtttg caatattttt tattatttta agtggatgtt caagtaataa gaaatcggaa
    10501 gctaaagaag ataatagttt attgcaacca ggaactgttt ggaaagaaga agtaggtggt
    10561 cttattcata atttaaaaat tatagatgat actacctggg aatattctga aagtgtatgg
    10621 catccagatc ctgtacaaat aacagttaag aggcaaaaag actatgaagg tttagaaaga
    10681 tacaaaatag tagattctgc aggcattagg gaatttataa acaaacctga ttcattattt
    10741 attgttgttc cctatgagaa ggatggcgta aaaaaaataa tttttcttgg atctagcaag
    10801 gatgagaagc gaacgaaaga gaaattaatt catgatggat cagagttaaa taaatacaaa
    10861 ttacaaaaga ctagtgagtg attgctagtc tttttattta tcctgaagga ggaatcacag
    10921 tggcaaaagt taaaacacca ttcgaaaaag ccgcgtctaa aatcttagaa cgccgtggtg
    10981 tttctgaaat acagtggaaa aaagaagttc ttgaaaaagc acaatttgac ctattacgag
    11041 gaaaagataa ggacttagaa gaatatatct atcaacgtga acgtgaaaaa cttgtattcg
    11101 atgaaattat taaacattaa aaggagcgaa taatcttatg tttgaatcaa ttacaatgaa
    11161 ttttttaact caaccgaaag tagtagctgc agttgatatt tttagagcat tacaaactgc
    11221 cgcaactagt ttgaaaaata atctgatgat ttttgctgct gttgcggcag tggcttccat
    11281 tgctcttgga ggtattttct tcttctttgg gcgaagagga gctgaaaacg ggaagacaca
    11341 cgtaggttat gtattgttgg gggtaggagt tattttagct gccgcttcta ttgtagtttc
    11401 aatgttctca atgtttggac aaaccccaca aggactataa aaaaaacaag taggaggagt
    11461 aaaaagtacg tggctacttt ttactccttt ttttatgaaa ggagaatatt catgtttttt
    11521 acgatttcag gaaatgcaaa attggataaa gagttaataa gaaaagcaac gaagaaagtg
    11581 ggaaagcgta cttccttttt accgaaaaca attaaatatt cggtcattga aacggaagaa
    11641 cagctacatg tgttaggaca agtgttagaa caagagaatc aatcttctgt taaattgact
    11701 ctcacgttac atgataaaga aggaaaaata gaaaaggctg gcacagaata cgtttttggc
    11761 ccactggatt atcaaatttc agaaggtttt tatcctacat tgatagattt aatccgcctt
    11821 gatgtgttta aagatacgga tgaaactatg agtgtggaag agtcttatcg aaacgaaaaa
    11881 cttttacaag ttttagaaga agagttagag agcgaccaaa gaaaagtgaa tcaagtgagt
    11941 gaagtcgaag taaatgaaga agtaccatgg gaacgaccaa tatttccaaa gaaggagtca
    12001 aaagaaaaag agacagtcta tgttgacgaa caagctgata ttccgactca gaaatcgata
    12061 tttgaggaag agaacgatgt gatgggtcaa ttggaggtag atccctttga agaaacaatg
    12121 gaggatgaag agacggacga agtagaagtt gatccagttg aagctaatga agagtcaaca
    12181 tggactgaag gggattttca ggaatatatt gaaccactta tcagtgaaga agtagaaaaa
    12241 aacgaaacag agatacaaaa ggcaactgta tttgattata ttttggatca atatccgcca
    12301 gagcatgaat ggatgaaaga aaaaaacaaa ttatttttaa atgagcttta ttcgaaatat
    12361 caattacccc aaacctatca agcattttta gagagtaaag aagaattaat ctcacagggt
    12421 aaaggcacac ttttagaaca gttagagtct gtccaatcaa caaattggca tgagcttgga
    12481 caagaagatt tattagccac ctttgaagaa cgtcaacaag aaacagatca agaaattaat
    12541 gagtatgcga cggaacagtc gtctaattgg gagaaagtaa aagaggaact aaataaagaa
    12601 gaacagcgag tgattgaaga agaagtacaa aaagtcaaag agcggtacga aaagaaacgc
    12661 gacaatgctt attccttgtg tgtagaaaag atcaacaatt ttactaagac gaatcaagaa
    12721 gcgttatcga aagaaaaaga aaacttgtta aaagaacgta ttaaagaacg aaaagaagag
    12781 ttctaccgcg gtttacgtac agataagcta aagattagca aagaattaaa tgaacaactg
    12841 aataagctat atcaaacaac gactgaagtt cttatggaaa aacacgaaca gatccagcaa
    12901 gaattacaag tacaaattcc aaaatggaaa aaagagtatg aaattcaaca acaagaattg
    12961 aaagaacaag aagaaaaaca acggcaaaaa gaaaaagagc gtgaacaatt agaaataaga
    13021 aaacgggaac aggatcttgc ggaacaaaaa atggcgatag aaaaacaacg gttagagcaa
    13081 gaaaaggaaa aagaagcagc tgctcgtaag gaacgtcaag aacaaatgga tacattgcgt
    13141 caagcacagt tgaattatct agcacaacct caaccagtag ctgctgttca aacgccagtg
    13201 aacaaccaag caaatttaac tccttcaccc cagcgttcct ttaacggatg gatggtggga
    13261 tgtattgcaa gtttaagctt gcttctaggt ggaggaacag tatttgcttt taatcatatc
    13321 aatcaatctt cacaggctga ttctgtagcc acaacggcgt caatcaagga agaagcccag
    13381 gaacaagcaa gaaaagagta tgaacaacaa ttagaagaat tattgaatcg taccaatcag
    13441 tcaacaacag agtcaagcat ggaaaatagc aaagagagtg attctagtaa ggctaaagaa
    13501 ggatcaagcg attcaactgt acaatcaaac acttccgaaa gttcagaatc aaatgaggtg
    13561 aaataatgcg tgttagagaa acaacgaagc gaaaagacaa acgattcaat ttagaaaaga
    13621 ataggtatct ttctcatttc tatcgctata ctccctcagg agaagagcta ccagaaaaac
    13681 atgacaaacg catttcgaaa aagatttttt atagtggact tgtatacctc cctttagagt
    13741 atttagtaac aagagtacca gaagttttca accgattgga aattgttaag ggaagcttac
    13801 aaggaataaa tctgtttcaa ttacaggaaa tgccacaggt ctttcatttt gattcagctg
    13861 gtttttggaa tccatttact cctacaacta gtttgggtac acgtttagca atttcgacag
    13921 tttgtgtggt tccagctgta cttatgggat tgaaaatgga catgtggttt cgtcctctag
    13981 cagatggaca aaaaggggat aatcgattaa tgaccttaaa agaaatccaa cagatttatc
    14041 cgcaaatacc tgaaacaaaa actacttttc caggttatgg aggtattcca atcacacatt
    14101 acaaaaaata ttggtacatt caaacagata ctgttaatac gtgtattgtt ggtacctctc
    14161 gatcagggaa aggtcagatt gaagtgttag ccaccattga caatttatct cgtgcagaaa
    14221 aacaaagttc aatggtcgtt aatgatccaa aaacagaatt atatgttggt agtaaagatg
    14281 aactagaagc tagagggtat gatgtatatg cctttaatat caccgatcca ctgcaatcaa
    14341 tgagtgataa tccattggca ctcatcgtga aatactggaa acgaggagac atagatactg
    14401 caacacagtt aaccaatact ttcacaaata cgatttatta tgatgccaac gcttcagata
    14461 acaaatactt taacgaaaac gcgcagaaag ctgtcaatgc gcttattttt gcgttgttag
    14521 aacaagctga tcaaacagga gattactcca aattgacacc gaataatctc gttgagcttc
    14581 tttctgagat tggcggtttt aattattatc cagatccaga caatgaattc aaacagttga
    14641 atgccttaga tgaatttatg aatcagttgc cacctggaaa cattgcaaaa aaacaatatg
    14701 gggcaacgaa aattggatct gataaagcga agggaaatat tttatcaacg gcaatcacgg
    14761 gattgaatcc atttactcta acgaaaattg caaagatgac ttctcaatca acgattgatt
    14821 ataaatcggt tgggtttcca aaatacttag atttgaaatt acatgaaagt cttttaaatc
    14881 aacgagtcgt agtagaattt tatcataagg gaaagaaatt tcatgaagaa cgtgtcaaag
    14941 taggctataa aggatttagt gaaatcaatt ttgattgtca tttagttgca ggtgatcaag
    15001 tttgtttaaa atacgatcat caaggaaaag aatatgtggc ttggtatgaa ttttctcaaa
    15061 gagtattgaa agacgaagag ggtaatatcg tttatcggaa aaaacgagga gaaacccatt
    15121 tgccagaatt agaaaaagaa gccgttctaa cattagatga ggaaaaaagt aacttattag
    15181 tagagtctat tcggatgcac tataccgata aaccaatggc tatttttatg cttacacctg
    15241 attatgatcc atctaaccat atcattgtta gtatttttct tagtcagtta tataaagaat
    15301 tatctacgca atgtgttaag actaaaggag ataaatgtca ccgcaggatt cattttattt
    15361 tagatgaatt tgggaatatg ccaccaatgg ataatatgga aggaatcatg acggtcacag
    15421 ctggccgaaa catgttgtgg gatctgttta ttcaatctta tcagcaactt tatgcgaagt
    15481 atggagaaca aaacgcagcg attatcaaag aaaattgtca gacacatatt tatattatgt
    15541 cgacagatga taataccatc gaagaatttt ctaaaaaagt ggggaataag acggttgaac
    15601 aagagaacag taccatcaat tcattaggta tgaatactca tatcaatcgc aatgttgatt
    15661 cagatcgtat cttaactcct gaaagaattt ctactttttt agaaggagaa acgattgtac
    15721 tcgctccttt aaaacgacaa gatttgaaag gtcataaaat acggccgtat cctattttta
    15781 atcatgggga aacgaatctt ccttatgctt atcaatatat tgaagatttc aatccatcgt
    15841 gggatatcaa tgatatagac atacattgta cacatgcgga cttggatctt aaggctttac
    15901 aaattgatta tgattatttc ctaccagatg aatatgctag aaatctctat catggactgc
    15961 atgatcaatt agaagaaaaa aacgaagaaa atatgtttta taaaatcgtt caatctcttg
    16021 agaatgagac ggctaaaaaa ttgttaattc aattttacga agaacaagca gcagatcgac
    16081 ttcaagaata tgttgaaaag ttgatcaaaa ataaagaaat ttctgaaatg attgggaaac
    16141 ggttgttatt ggaatctgaa aaggtggtga aagaatgatc attggaggaa ttttttctgg
    16201 catagccgac tggtggtcag gcttattcgg agaaggggat gcaaacccca aaaccgtaac
    16261 gaaagtctta caagattatg ggaattatct gcagtatcaa gatgtattta gttggatttg
    16321 gaacactttt ctgtggggat tgattaaagg attatataat ctcaatactt tactagaaaa
    16381 aaccatttat caaagctttg atttaaaaga cttattgaac gccgctgggg taaatgcact
    16441 atttcaaagt ttgattagta aggtattggc tcttttttgt gtcgtcacat taatttattt
    16501 aggcttgaaa ttttttatga gtaaacaccc accaaaagtc aaaaatatct tggttaattt
    16561 atttatggcc atgatcttga tttttggtgg tagttcttta atcgatgaag ggttaaatat
    16621 cagtaaaagc ttttacggag acgtcactgc agcaaataaa gcagataaaa cgggatcacc
    16681 tgcttttcaa attattcaaa ataatgtgat cgatgtcagt acgttactag caactgaccc
    16741 gaataaagta gaatccctac caacagataa aagaaatgct ttaacagcga aaaattttaa
    16801 aacagcgaat atcaatggga ttattactcc tgaacaagct aaaggtatgg caggtaatga
    16861 taaaagtggg ttatcagata tacaaaagga tcggttaaag gcattacaat accgattgga
    16921 actagatgat aatgggaaag aaattcctgt tcaaattagt gacgaaggct tagcaaaata
    16981 tgtctatact tcagggtatc gtcgatacag tagtaaacca ggtattattt tagctggtga
    17041 atcttctttg gcagtagcct atttatttat cctatttacg atcgttactt gcattattga
    17101 gctggtattt aaaaaattct atttagtgat tgccgcgtca acggactttg agacaggtca
    17161 acggatgaaa agtgcgatag aagatatttc tcaatccttc ttgttattgg cgtttacgat
    17221 tttagaatta cgaatctata ccttaatgct ttcaggtatc ggtgatttgc atgcggcagg
    17281 aaagatcaat ggattcttgt atgtcgttgc tttgattgta ctaaccattg cgctattcaa
    17341 aggctctcaa tcagttacga aaattttcgg cgttgataca agcctgaaaa acggcagtaa
    17401 ttctcttctt agcatgtttg ccttaggaaa tattgcgaaa aatgtgggta gcggagctaa
    17461 aaaccttgca agtgctggta agagtggttt aagccattta aatgatattc gtaaaaatgg
    17521 atttggccat cgaaaagatg ttacccctga taacggggaa gaacaatcag acgataaagc
    17581 tacaactgta cctaatagta agaaaacagt ttccgaaatg ttcgggaaag cgaaaaatgg
    17641 tgtttctaaa gccgcagaag gtcttggtta cgtcaatgaa cgtgggttaa aaggaacagc
    17701 tgaagatctt acagaaaaag ccaaagacac cttagaagat aaattagacg gcacgagagc
    17761 taaagaattg gctgaagctg tgaagaatcc atccgaagca ctagaaaaac aaaaagaaaa
    17821 agcttcagaa gtcaaagaag gactaaaaga tgcgtatggt gaaggacaag tcaatgcagc
    17881 agtgaaagct aatacaacaa ccgaaaaaga aaatcaagaa ggtatagtga ttccttcagt
    17941 caaagatcac caagaagcgt tgcactctaa tgaaaattcg attgaagaga aaaattccat
    18001 agaagaaaga aattcagaag aaggaaaaaa tggacagtta ccaaaaactg atccagaaca
    18061 atatcgctat aaacctaaca gtgaccttga aaatgagaag cgaatttcag atttatcaaa
    18121 agtaaatcaa gaagacaaag cagaaaatga gggacagtta ccaaaaactg atccagaaca
    18181 atatcggtat agatctgtta actccgaaaa tgaaaaacgc atttcagatt taccaaaacg
    18241 aaatcaagaa gtacaaacgg atgaggattc catcgttctg ccaaatgata atggaatcga
    18301 agttggaact gaaaaatcaa cgaaaattgg tttacctaag gcacaaaatt taccaatgaa
    18361 agatagtcgt ttgaatcaat tcaaggataa tgatcaaagt tttgttcatc aatcacctgc
    18421 aggtactgtt caaatgaaac caagtcgttt gcctgaaaat ggaaatctga caactgtttc
    18481 ttacaattta tctgaaagtg gtgagacagg gaatgcgtcc gttcaaaatg ttgtgacaag
    18541 agaagctgca ccaaaaccta ttcaaaacgt tcatacaggt tacaagccag caaatgtggc
    18601 atttactgca ccaacaaaaa taaatgtgac aagagaaaca aaaaacagga acaatgcatt
    18661 gaaagaacaa ctattgaggg atatcaaaca ggatatcaaa ccataaaagg cctacaagtt
    18721 aggccttttt tctatgtatg aaaaggagaa atcatttatg gataaatata gtaacatgaa
    18781 aaaccctcca agatttagtg gagaatggaa gtttttcgct tttttctttt tagatctatt
    18841 aacgcttggt ggctgctttt tttgggcgaa aatggtcaat aatcttgtcc acttaccact
    18901 cttttttgca ctcttgaatt atggcttatg gattgcgata gcgttagtga ttgtttggcg
    18961 accctataat aacccaggtc aacgacaaat ttttgcgatt atcgagtcta ttttttttgc
    19021 ggacaagaac cattatcatg cgttagatcc taaccgaaat tatcatggta cgtataagga
    19081 ggatgaataa atgcgagtca aaggacaaaa ttcagttgaa aggaaatcca ttttgcagtt
    19141 tggaaaacca gaagagcgct ctatcttgga cttatttcca tataaatcct gtacagaaga
    19201 gggagtctta attactagag aagatcattt tcaacgttat tatcgtgtag tttctacaga
    19261 cgtggaagga ttaaatgaac aagagaaact agaacgaatg aatcagttga ccaccgtcat
    19321 gcgtacgtat gtgccaacaa ttaagttagt tagtttgacc acagagacgg atctaacgga
    19381 acaaatcgtg caaaagcggc aattattaga taaaaatcgt atggaacagt ccttaggaag
    19441 aaatctacaa cgattaaaaa aatatgaaaa gaaattagtc gaagaaatcc aggaattgaa
    19501 acgagcagaa caagaacgtc ctgacttaag cttctttttc gtcattgaag gaaagacaat
    19561 caaggattta caaaccagaa accgtcagtt aatgcgatct agtggcattt tagggttaaa
    19621 accactctac aaaaaagaat tgatcaagat tctgtatcga atgaacaata tgaatgatga
    19681 gtaggaatgg agaagagaga atgaaatatc cgttgatatg gttagaaaaa gatgttccga
    19741 aggaagagac cactagaaaa tttcctcaca ctttaagcct gggtaagttg ggaaaagggc
    19801 aatcttttaa ccaagtaaag aatcaaacaa gaagaggtga ctgtacatgt tagaagcaat
    19861 taaaacagct ttttcaccac aaaatgatca aaaagtactt gatccattaa agcaactagg
    19921 aaatcgggaa atagaaattg taaaatccaa agggtatgat ttagattttt taaaacaagt
    19981 gatgccacaa ggtgggttaa attttgatga agaatatgtg acgtatggta atggttattc
    20041 acgatgtgtg acagtttatc actacacaaa aaatcctacg cttttttggt tggccaatct
    20101 catgaacaat cctcatacag tggccacact cgatgttagc acagatgaaa agcaaaaagt
    20161 gattgatcaa atcaatcgtg ccttagatga attaaatgat cgatcagata atgaacgaaa
    20221 agcgactgac cgaaataaaa gcgcggaaga atacctggaa ctagcggaat atgctagaaa
    20281 attgacttct aatggggaaa tttctaagca agtcaaagtg agagtacatg tttacggaaa
    20341 cacgattcaa gaagtagatg aacgagcgca ggaactgatc gaaaatctca aagggatgga
    20401 ttatcgtgcg gtgacttatc tgcataaaac caaacgagat tggcaatcga tgtttaccat
    20461 gttcgatgaa caagctgaat ggttcggaaa tatcaaaggg caatcactac catcgttaaa
    20521 cattggcgga ggtttcccgt tccaccacca aacgctaaaa gatccaggag ggacacaata
    20581 tggggaaacc atgacaggcg gcccatttgt ctggaatgcg acgtataaaa atagtgtacg
    20641 gttgtctact aataccttgc ttctaggaat gatgggagct ggtaaatcga ccgttcttaa
    20701 aatgatcgcg gaagcccatt tagctgcagg agactttatt tggggctttg aaaagggaaa
    20761 agactttatt cctttcttaa aggaatataa cggtattatg gtgcgtttag acggatctga
    20821 cggaatgatc aatcctttag agatatttgc cacacggacg tatgatgaag catccagttt
    20881 atatgacgat ggatcagtca aagatttgaa gatcaatgaa gcagcgtcct atcaaacaca
    20941 cttagacaaa gtggtctatc aggtacagtt agtgagtcct cagctaaaag gaacgatgaa
    21001 agcagaattt aagacctact taaatcgttt ttatgaagag tatggaacag taccacgcgg
    21061 ctttacttcc tcaaattcgc gttcaaatac tgatacgcaa gtcacaggga aagatccaga
    21121 agcttatccg acgttcaaag aatttttaga ctttttagga caactggaat taccaggtgc
    21181 ttcccaagag aaaaagaatc gaaaagaaga aatggaatcc atcgtagaat ccttatgtga
    21241 gacgtatggc atgatttttg atggtcatag tacgattcga caccttgacc aacaacagtt
    21301 ggtctttttt gacattgact ctattacagg cttagatcga ggggtacaac aatgtcaaat
    21361 gtacatggct atgacgttga tttggaatca ggcattgatt caaggaagaa gacaacgacg
    21421 tttaattaaa gaaaaacaaa tggaagaaca agacttcaaa cgcttcttgg cgatgattga
    21481 tgagtgccat aatggtgcga cctgttctgc agtgaaaagc tgtgggttgc aagtagtcta
    21541 aaaactattg taataactct tataacgaga agtcaaatga gtgggtaacg ccacaaaggg
    21601 cttccactga tactccgaca tgcaacaagt agtttatgca gaccaacgtt gtgtgaagct
    21661 cggtgaagtc ggctgaagga taccgtaagg taaaagaaat agtgaatacc acgaaagttc
    21721 tctagaggta aagggtgtat ccgataagtc gggtgtccaa aaaaacttaa tatggtgagt
    21781 atgtacaaac gtactgacga actagcgaat gtactgtccg aaaggcatag ctcctagaaa
    21841 tgggagagac agtgagcaaa aaatcactca ttgtgctaac ttgtggttga gtaaaagtcg
    21901 ttgttatgaa agaccatgct gcgttacagg cgtaggagaa gttagggggc aaaagctagc
    21961 atctaagtta aggcatgtgg atagatataa gggaacaggg gaagccatca acatggagag
    22021 tgcatactcg atgaagtggt agtaagaaat atgtgaatga caaataactt actttagtga
    22081 tggtgaaagt agagtcacga ctgatgaagg ttctgtaatg gaactggagg aacaggctcg
    22141 attcgtctgt aaaagtcatg aaacccgttc aaccatggga ttctcgtatg actaaaaagg
    22201 tcacgagcca attggagcgt gataaaccat gacaaaagaa acaagtccaa aagataggaa
    22261 gttgcggcat gcagaatatt atggcatgac ggaaatcttc gataagatat actccaaaag
    22321 tttgaaggga gataatttca agaatctaat gcagattatc atttctgata acaacattgt
    22381 cctagcatat cgtaatatca agagaaatca cggtagtgtg acaagaggag aagatggagt
    22441 aacgattaaa gatattgaaa atctcaaccg taatgaattc gtagaaattg ttaggaagcg
    22501 attcgcacat tataaccctc ggaaagtaag gcgtgtagaa attcctaaat ataatggtaa
    22561 attaagaccg ttgggtattc catcaatatg ggatagaatc gcgcagcaat gcatcttaca
    22621 agtattagaa ccaatatgtg aagcaaaatt caataatcac agctatggtt tccgcccaaa
    22681 tagatcagca gaacacgcaa tagcagattg tatggcccga atgaatcaaa gtcacatgga
    22741 atatgtggta gatgtagata tcaaaggttt cttcgacgaa gtcaatcata ctaaactcat
    22801 gagacaaatc tggtctatgg gtatacgaga caaacagctt ctcttaatca ttaggaaaat
    22861 gttaagagca cccgtagtac ttccaaatgg gaagttgata tacccaacaa aaggaacacc
    22921 acaaggcgga atacttagcc cattacttgc gaacatcaat ctcaacgaat ttgactggtg
    22981 gatagcaaat cagtgggagg aaaaagatgc caaagagcta aaacatcatt ttaggaaaaa
    23041 tggagcttgg gataaaggta atatctatca caagctcaga aagagtacaa ctctaaaaga
    23101 aatataccta gtgagatacg cagatgattt taaaattttc tgtagaaatt ataatgaagc
    23161 gaaaaaagtt ttctatgcaa ctaaaatgtg gttacaggag cgtctaaagt taccaatatc
    23221 tgaagaaaag tcaaaaatca caaatctttc aaaagaagaa agtgaatttt taggctttac
    23281 gatgaaatta gtgaccaaag gtaacaaaaa ggttgttaat acgcatatag gtacaaaagc
    23341 acaaagcatg attaaagtga aactaaaaaa acaaatcaaa cgaattcaaa aaagtcctaa
    23401 tagcaaagat acctttatct atattggagt ctataacagt atggttattg ggattcataa
    23461 ttacttcaaa attgcgactc attgtagtgt agcaatgcga aaaattgcaa gagaactcag
    23521 accaagtttt gaaatacgac tacgagaaca aggaatctca aaagaaggac aatacggaaa
    23581 gtacgaagga tacaagcctt acctacggtc aggacaaata cgatatataa gagggtttcc
    23641 aatattacca gtagggtata ttcaacacag acatccgatg tgtaaaaaaa gaaatattaa
    23701 taaatacacc gaagaaggta gggaagccat tcataagaag caacaaaccg tagaagaatg
    23761 gaagatatcg tggttgtgta gcaatccagt gcttagtaag agagcaacga ttgaatttgc
    23821 ggacaatcga atttcaaaat tcatcgctca aaagggaaga tgtgcagtaa caggtgagga
    23881 gcttatctta tctgaaatgc attgtcatca tattatccct tatcatgaaa gcaaaagtga
    23941 tagctatgaa aacctagtga ttgttactga ggaggttcat cgggtaatac acgctacaca
    24001 aagtgaaacg attgaggaac ttctaaaata tctaaaatta aatccaaaac aaaaagaaaa
    24061 actcaatgag ttgagattaa aagttggtaa cgaagaaata tcttaataaa tatttgtagg
    24121 acgggaaaac agaatgtctt ttatagacga gggaacgccg tatgcggtga aagtcgcacg
    24181 tacggtgtgg actgggggaa aagacggaga taatatcaaa ctcttaccta tcagtatcga
    24241 ttaatccaga ttttattgaa acggtaaatt atgtcacaaa ctttcaacga gaaatgcgta
    24301 aagtagaagc aatgactgtt ttagctacac aatccccaca agaaatggtg ccagaaaata
    24361 tgtcttctga taacttctcg aaactgaaga aagtctttga attttcttct acgaagatct
    24421 ttatgcgtat ggatgaatct attttacgtc atatagaagg attagtgggt aaatcaatga
    24481 cgaactcaga attagaatcc attcctcaac aaaatcaagg agacgctgtg atcaattttg
    24541 gctcaaaaga aaccattcgt gttcatttta gtccgaacaa acgtcaatta aaattgtttg
    24601 atggaggaaa ataatggaaa accaaaatga aagtttaatc aagcagtatg ttaagcgtcg
    24661 tgcaaagcgg cgcttatttt tgtggttgtt tggtacttct gcaggtttga ttactatttt
    24721 gatcactgtg ttcgtgaccc tatttctaat acttgcggcg ggctcgattg ataattcgga
    24781 ttcagattca agcagtggtg gcgaagcttt tacaggagaa tattcggaag gcttaccgat
    24841 ttataaagaa atcaaaggaa gaggcccttt ttctgatgag attgcgcaat atgccgtagg
    24901 tgcagcagtg aaatataaat tattaccaag tgtgatcctt tcgcaatacg gttacgaatc
    24961 agcgtttggt acatcggcgt ccgcaagaaa tgatctcaac tattttggga ttacttggtt
    25021 tgatggttgt ctgtttccta aaggaactgc acgaggaatt ggtggcattg aaggtggctg
    25081 gtacatgaag tttcccaact caaaagctgc attttcctat tacggcttta tggtagccac
    25141 acaatccaat tttaatgctt gtgtaggaaa taaaagccct ggcgcaagct tattgatctt
    25201 aggacgaggc ggttatgccg ctgcaggaat tacggaagat agtccgtatt atacaggttg
    25261 tatgtcaatt atcacttcta ataaattaac cgaatatgat gaattcgcca tcaaacattg
    25321 gggtgaaggt ggcaataaca acggcacgat tactggtgaa tggacgaatc cattccctgg
    25381 cagcagttta gataaaagtt ccttcagcgg tggtcaatta tttggaacga atccaggcgg
    25441 tgaattccgt ccgaacggct tccatgatgg attagatttt ggttcagttg atcatccagg
    25501 aagtgagatt catgcggttc atggcggaaa agtcgtgtac gttggcaatc ctggtatttc
    25561 aggtttgggc gcgtgtgtga tcgtgattaa ttacgatggc ttgaatatgg tgtatcaaga
    25621 atttgcaaat agtacaggaa attctcgagt gaaagtaggc gatcaagtga aagttggtca
    25681 agtaattggg atacgagata cagcacactt gcatttagga tttactcgaa tggattggcg
    25741 tcaagcacaa ggacatgcat tcattgatga tggtacatgg atcgatccgt taccattttt
    25801 gaatagcagt aaaaaataga tcaggagaaa aaatatggaa aataaaacgt taaaaatcgt
    25861 cttaacagct gcaattagcg ttagtttaat cggcaatatt gcgttgtttt ggcagctgaa
    25921 agaggaacaa gcagcaaata cctataaaat tgaagaagtg gctaaaaacg cgtctgagaa
    25981 gctcaaaaaa aattcctctg aggcacaggc gaagatcgac aagttaaatc aaaccatcaa
    26041 tgagaaaaac caagaaatcg atcagctgaa gcaacaagat ggcacaaaag caaaagagga
    26101 agtaactgaa gcacaaagaa agacggcaga agagttcggt caattggcca ttgataaatc
    26161 attaagcaga aatgaattaa cggaaaaact aaaagatgtc gcaacccaag aagtgatcga
    26221 caaattaagt cctacagacg atgatcacca acatgatgat tacggaagtt actcctttac
    26281 attaggtgac gtgcaaactt acgctcaaac aagcagtgta aaagaggaac aaaattttgt
    26341 cgtatttgtt gattatacaa tcggcaatcc gcaattcaaa gatgtcaaac cacaaaaaat
    26401 aaaaggtgga ttaactgtca ctgaaaaaca agtagatggt caatggaaag tcatagactt
    26461 tagttatttc actcgttagg aggaagaaaa tgagtaagaa aaaaggagaa agacaacgtt
    26521 tcaaaaaacg aaaaagttat cttagatggc tgggaatctt tatgattctc ggctttttga
    26581 ttcttgctag tccgatttat acgaatcaga aggtagatat accagatgag gtaagcacga
    26641 acgccttttt aaacacgaaa gagctgacaa tggtttcaaa agaaaaagga gcgaaccagc
    26701 aattagtttt tgaatttgcg atcgatgatg aagatcaaag cttgttaaaa gaactggcta
    26761 atcttaatta tcgcgtagaa gtaaagacag ctaaagggga ttatcaagca atacgagcca
    26821 aagtgattaa ggtgtctgat gattattttg cagtgaagct aacgcatgta ccagaagaat
    26881 atatcgccat gcgtttaacc attattcctg aaaaaattga tccaaaagtg gatatgcaag
    26941 aaccacaaga tctgatttat tacgtgcacg aagataaggt taaagatcaa gtgaaagatg
    27001 aagattatga acaacacgcg atcaattaca aagtgaaagg atacaagaaa gaacaaaaag
    27061 cagctcaaaa acaaatggaa tctttccaag caaccattga tttgaatgaa gaattagtga
    27121 agaagctaaa agatcaattg ccttatcaag tcgcagatga tcaggaaaat actgaaaaca
    27181 agattaatag ctaccagcaa gaaattgaaa catcgaaaac gcaactgaaa gatcaagaag
    27241 agatcattca aaaacttcaa gaaaaaattg atcgtatgac aaaagaaaag atttagaagg
    27301 acaggtggag tgaaaaaatg aagcgaacaa acttggctga caaagatact ttcgctgagc
    27361 agaaagtatt gattaaagcc tatctcaaag ttgtaaaagc taaagaaata tgtcgagaag
    27421 tgaatttatc taacacaaca atatctgagt tgaagaatgg tcgaaagaat ttagatactt
    27481 taaaattcag atcatttgaa gctctatatg tagctgctaa aaaatatttt acagctgata
    27541 acaaataaaa aacaggagta ttttatgtga aaaaagtata taaaaaatgc ttatgaatca
    27601 gctatatcac cttaaccatt tgttaatgag atgggagaaa acattaacca cgattctaac
    27661 gcaaacatca agcacataaa acatttaaca ctcctccttt ctggctgaag atttcaacca
    27721 aacaggaaga cagcgtatca attatagcat acactttttc ttaaattcat ttttcatatg
    27781 acaaaatatt tccttctaat cgacaaagtt agaaggattt tttattgtct aaatacacaa
    27841 ataaccttaa tttttaaaga gcataaggaa acgtgatatc actttctcac gaaaggtgtt
    27901 aggtaggctg tgcctaccac gtttctaaga aagataaatg atatcaaaat tttggatttg
    27961 tgtgtagatt tgtgggtagg atgaaagcga atgtgggcag taaaataagc gtttgtgtgt
    28021 agcaaaatca aaaaatgtgt agggatatgt gtagctacac atatccctac acataataaa
    28081 tttggaggaa aatatggact tattaattag aaatattgaa gaaaagtata ttaataaaat
    28141 tgacaaacgt tgtgaagaac ttacggtcaa gactggcaaa aaatggagtc gaaatcaata
    28201 cctaaaagtt ttgatcgaaa atgattttga tcacgctctt ctgaattata aaaaagacca
    28261 atttgatcgg ttgttagaaa aatttgtgga tattcaaact tataatacga aaactttaga
    28321 agaatatatt gcaacaaata atcaattaat tggtttgtta attgaataat aaaaatagta
    28381 ttgtgaattt ataaaaggag gaaatcaatt ttggcaaaag caacaagaaa acatgtatat
    28441 ctttacgata tgcatatcga agtacttgat gaaataattt ctactcatga agatatatct
    28501 aattatagtg aagcagtcag acatttgtgt atgatgtatg atccaaaaat cgaagaaaaa
    28561 ataaatccaa ttcaaattaa attgaatgca atgggaaaag aaatttctat tttaaccgaa
    28621 atggtttcag agtttgcgga tcttcatttg aaagatacag gaatactaat tggtacaaat
    28681 tcaactgttt atcaagaagc agaagaacgt gtggaaggaa aaattaaatc caatcaaacc
    28741 aaaaaatatt cttctaagtt aaagaaacca gtgaatgaat ctgttcatac cagagtagat
    28801 ccatttgatc caacgaagat tctaaaaaga taaaagtaga aggagaacgt gatgaataaa
    28861 tcgcctgcag ttattcttac ttgtcaattt accttgccca ataaaaaatc tttttcaaca
    28921 tacattgatt atatgacaag agaaaaagca ctggaagaaa tggaacgacg tacgcccgaa
    28981 gaagaacagg aactgcaacg aatcaagaat gctttagagg atttttatat tcctgaagga
    29041 gaaacttttt cggaaagtaa gacggataaa gaattcagtg aaacgtcgaa tgaagctcaa
    29101 tcgttgatgg aacatggaat caattttgac aagttagagg aacaagattt taccaaatat
    29161 ctttcttata tgacccgtca ctatgcgtta gaacaaaaga aagaactgac gcaaagggaa
    29221 caaaaagaat accaacggtt gaaccaagca atgaaaaaat atcaaacaga gccacataaa
    29281 aaagcttcag gaaatcttcc tggagttttt tctatggctt cagacgaagt gaaaggaagt
    29341 gatctgaagg agatcaaaca aatcttccaa agaggacaac aaaaaggctc aatcatttat
    29401 caagatgtta tctctcacga caattcgtat cttgaaaaaa tgggcttgta cgatcccaaa
    29461 acagatacct tagatgaaga aggtttgaaa gcagctggaa gaaaaatgat ggagacatta
    29521 ttcgagaaag aacaattgaa tgaaacaggt tactggatgg caacgattca ccgtaacact
    29581 aagcatatcc atattcattt tgcagttgtt gaaaaagaaa atacgcgtaa accgtatact
    29641 gagattgaaa atggtgtgag ttatatcgta ccgaagggga agagaagaca agcaacatta
    29701 gacgcaatga aaaccaatta tgtacatgag ttagaacgtt tctcttatga aaaggatcga
    29761 atgatcggta tggagaaacg aaatttattg acacgaaaaa gtgatttgcg taacacctta
    29821 acaaaagtag taaaagaacc agcacgttac gatcgacacg ccatgaactt attaaatgaa
    29881 gtatataaaa ccctacctga aaaacgttcg gaatggaatt atggagatga aaatcgaacc
    29941 aagctttcac catttacaag gggaaaactt gatgagttaa ccaaatatgt tttaacaaaa
    30001 gaacctggat atcaagaata tatacagatt tcaaaagcat tgaaagaaga agccaaaagc
    30061 ctttatgggg aaagcaaaag agaaacaaag aatgcagaaa aaaacgccct ctttgattta
    30121 aaaaagcgca caggaaatgc gattttaaca gaactgaaga gacaggatag tcaaataaaa
    30181 ccattaatag aaagtgtcaa tcagatgcaa tctatttaca atgaacgatc ctttcgaaca
    30241 ctcaaacagt tgccaacaaa tgaatacaag tatagttatc aagaattcgt aaagcgtagg
    30301 aaacaattcc aacaaagagt tgtcacgcag cgaagcttgc gtcaattaaa tcgaacaatt
    30361 aatgcacatg aagaaaaata ccacgcaagt aaagcatatg aagagcaaca gcgaagaatc
    30421 caaatggaac aagaaagaca ataggaggaa gagaaatgaa acaatattta gatcaatgga
    30481 aagtcataga aggatcacta aaagaagaac gaatcgaaca acttcctgat tgtttagaaa
    30541 aagagcattt gtttcagata agcgaaatgt tgagaaacga acagtttgat ccaaatcaat
    30601 ttttagttgt tgagtaccca gctactggtg tgtattgttg taatcatgtg aagggtgaaa
    30661 aatattttat tattcaagaa tacgaaggta agttagcgcc ttattatact acttgggaaa
    30721 taaatgaaga gggaatcaat aatttcccat gtgaaagcat tgaagagtct atttctctca
    30781 cggaatgtta aataaataga gacgtaaaag agtcctgaac gtgattgtcc aggactcttt
    30841 ttcaatgaat tgttttccgc caattcactg agtggtgcga ttcaatttta ttatatcaaa
    30901 agaaagtgag attccaaagt gaagggagga agaagaaatg actcagacaa cgattggtgt
    30961 tgtggttgaa aaaagcaggg ataatctcgt ttttgtgaca gaaattcaaa caggtagagc
    31021 ttttgtagtc acagacaaag ctgcaaaagc gtatcaaaat ggcaatattt taacgttaaa
    31081 tctgactacg aagatgttcg ttgatgcagt agaaaattat ccatttgttt agacaaacga
    31141 tattttacaa ggaggaaacg gacatggcag aagaaaaaga accactagat aatagtcggt
    31201 tgggaaagtc caaaagaaaa ttagtacggt tgcaaaacga attaaataat caatttcaaa
    31261 agctatttga tcaccaaaaa ttgacaaatg ggcaaccaat gaatgacaaa cgaaatggtc
    31321 gttcatggtt taaccagcaa gaaaaaatag aaaataaaat acaaagtttg agagaagaga
    31381 tcaagcaaca agaaaaacag gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg
    31441 gctataacaa gtacggcgga ctggatatga cgatcgataa tattccaaga atcaaagaag
    31501 aaatcgaacg ctttgaaaaa ggtgaatcga ctttttcagc cgccacgatc cgaaaatatc
    31561 aacggaagtt ggaaacatta gaacagctga aagaaaaatc agaaaaagga aaagaaaata
    31621 tcctccctga agtacaagcg atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc
    31681 caaccattta ttttttgaaa ggctatcgaa aagttgcttt agaactgaat gaaaacgggg
    31741 gatttgagga atcaaaaaaa taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg
    31801 aattacttga agaggctgca ccaactgatt ctctgaagga gaaagaaaaa gatcatttct
    31861 atcgagtaga attcaacgaa aatgatccag aaaaaggatt gaaaagttat ggtggggaga
    31921 tcgtaacatt agaattgatc gatcaactta aaacgttgga taaccaatac gctgcagaag
    31981 caggttatta taaattctat tttgaagaag tgaagaacgg agaagtagta gatcagatcc
    32041 gaatggatct aggggatggc gtcaacagta atcaccccat ctatttagag ttagcaaaat
    32101 tgtgcgaaca agtacctgag aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga
    32161 aagaacaaat gcagtcgaaa gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg
    32221 cagaagagat caaacaagaa gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa
    32281 agcataccca ttcttctgaa gacgtcaaag aacttttgaa ctttatgtct cgttttccag
    32341 aacgatcctt tagaaatcaa ttattgattg aggaacaatt tccaggtgca acggcttgtt
    32401 taggtcgtgc cgcgttgaaa aaagaaggaa tctatatcaa aaaaggggaa caagggaaca
    32461 agatctttgt cagaaaaacg gtcaaaggat tttatgagaa aggacgtggc tttgttcgag
    32521 aaacagaagc tacaccagaa gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca
    32581 aaaaacctta ctacacgatt gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg
    32641 aagactatcc gaaaatcttt ccgaaccgtg tctttgattt tcaattggat aaaaaaggac
    32701 agtcagaact tcaagctggg attcaagcgg tagccaacaa tattggaata caaattcgag
    32761 atatgacaga aagtgaagtc tatcaaagag aattaggaca agcacgtggg gcgtatgttc
    32821 gtaacgaatt tactggcgaa gaagaaatcg tcatgaatac tcgaaatact cctacgcagc
    32881 atttagctac aagtattcat gaattagccc atgcaagaat gcacaaattt tcagaattag
    32941 ataccgcaac taaagaattg caggcagaaa tgacttctta tattgtgtgc aaacattttg
    33001 gaatggacac gtcggagaaa gctattcctt atattgcagc atggacgaag aatggtcaga
    33061 cgttggatga taaggaagcg gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg
    33121 caaatgaatt cattcaaacc atttctaatg aaatcaatca atacagagaa agagaaccag
    33181 agatccaacc agcagaagtg aagcaggagc ataaaaatac tgtagaaacg gcagagaata
    33241 agaatattgt gcccgttggt tcactttgga tcgacaagaa agacgggaaa gtaatggaag
    33301 aagatacggg tcgaacgtta catttgaagg atgcggcgtt tattgaagat ccgcagcata
    33361 atcaagtgat tttaaaagga aaaagttatg gaaaagagat tcaaacggcg ttaccttcag
    33421 aagaatttgc gctaggaaat ccattccgat tttcacttga aacagtgtca gaagtggcca
    33481 aacgaaaaaa tgaaatctat tatgaacaac aattgtcaca aaaagaacgc gtataaaaca
    33541 agtaaaaaag actatctgaa aaacggatag tcttttttct tttgggaggt ttgaaaatga
    33601 ccgaaaaaaa aatgagtctt attgatcgat gtaaacagat cgatatagtg gattttgctc
    33661 gaaacaatgg aatggctgta gtcaataaag gacgagacta tcgcttagaa gaccatgatt
    33721 catttgtttt tgatcgaaga aaacaacgtt tttattggaa cagtcaaaat attagtggcg
    33781 acattattga attagctaaa ctttttttca tagacaaaga gatccaagac tctaagcaac
    33841 aatttaaagc agccactgat tttattctaa agaatgaaga caaaacagaa cgagtggaaa
    33901 atcttcattt tgaaacagaa aagtacaaag atcaccccgt tgattatcaa ccactcacag
    33961 aaaaaggaag gaattatctg aaggaagaac gaaaactacc agagtggctg attgattatg
    34021 cagaaaaaga agggttgatt gccgaactaa agccaaaata tgaacggcaa aatttcttag
    34081 ttcgagacga tcgtttagat catgcggtcg cctttttatg gaaagatcca cagacaaaag
    34141 agacagtagg ggcgagttat caaggaacgt tcattgatta tgaacgtttt ggtgagagag
    34201 gcacatataa gcatatagat aaaaattcga cagcgaatca tggctttaac ctaaagatcg
    34261 gtgaccctaa gcagttaaaa ttttttgaga gtagtattga tttacttagc tatgctgcac
    34321 tgaatcgtga tcagttaaac gatacgtggt tagtctctat ggaagggtta aaacaccatg
    34381 tgatcagtca ttattttggt gaggcagtat ctgaattgag aaagaaacaa gcctttcctc
    34441 aatccatcga gatttgtgtg gataatgacc gtgcaggaca tattttctac gaaaaagaac
    34501 aattaatggg cgcagtcgat ccatttacga atcagaaagt acgttgtgaa cgaggaatcg
    34561 ctaatgattg gcaagtgcca aaagaataca aaatcattta tgaagaagtc gcaaaagaag
    34621 aaaaggtcac accagaagct attatggcca tacacaagac agaaaacaat ctgcaattaa
    34681 cgaaccaact ggtttccgct cacaaagtaa atgcgttttt tggccagcag ctttctgtta
    34741 acgattcaat agaagccatt aatttgaaag atatttgtag agaagtcgca aaagaattaa
    34801 aagtttgtga acgcgtggat ggtacgtacg attttgatcg tttttatcaa gaaaaagggg
    34861 atattaatgc gcagattctt ttctcttata aagcagaaca atactacaaa ggctataaaa
    34921 atcatgaaca tgaatttata cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg
    34981 agatccagca acaagaaatc agaaagcaaa aacgtgccat gttgtttcaa caaggcagac
    35041 aacaagaaag ggagtgaaat cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca
    35101 caagcgaaag cttatgccga tagcttctct aaagccactc gaaaagacgg gtattttgaa
    35161 atccaagatc gattgtttcc tggtgaaact gtgatcacgt atggttttgg tcatttagtc
    35221 gaattagatt cgcccgatat gtacgatgaa aattggaaac aatggtcatt agaacatttg
    35281 cctattttcc ctacacatta tcactatcat gttcctaaag ataagaaaaa gcaattcaag
    35341 gtggtgaaac agcaacttca atcggccgac accattatta ttgcgacaga tagcgatcgt
    35401 gaaggggaac tgatcgcttg gacgatcatt caacaagtag gcgccgatca aggaaaaacc
    35461 tttaaaagac tttggatcaa ctccttagaa aaagaagcca tttatcaggg attccagcag
    35521 ttacgagacg caaaagagac ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc
    35581 gattggttaa ttgggatgaa cggtagccca ctgtatagtt tactcttgca gcaaaaaggc
    35641 ataccaggaa gcttttcctt aggcagagtc caaaccccaa cgctttacat gatttatcaa
    35701 ttgcaggaaa agattagaaa tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta
    35761 atcgctcaaa atggcgcatt tgatgcgaag cttgatccga atgaaaccca agcgacgcaa
    35821 gaagcatttg aagattattt aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg
    35881 attcgccaag ttgaaaccga gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc
    35941 agccttcagt ctaaaatgaa tcagctcatg aaagctagtg ccaaagacac tttggaagcc
    36001 atgcaaggct tgtatgaagg gaaatatcta agctatccac gaacagacac cccttatatt
    36061 acggaagggg agtatgcgta tttgttggat catttagatg aatacaagca ttttttaaag
    36121 gccgaatcaa tcccaacccc tattcatacg ccgaatagtc ggtatgtgaa taacaagaaa
    36181 gtccaggaac attacgcgat cattcctaca aaaacggtta tgacagcagc ggcttttgaa
    36241 caactaagtc cactgcagca ggccatttat gaacaagtgt taaaaacaac ggtggccatg
    36301 tttgcggaaa aatataccta tgaagaaaca acgatcttga cgcaagtcca gcagcttcag
    36361 ttaaaggcaa tcggaaaagt accgatagat ctaggttgga agaagttatt tggcaaagag
    36421 aacgacggaa aagagaagga agaagagccg ctacttccta aagtgacaaa aggagaaatg
    36481 gtgacggtag atctacaagt ccttgaaaaa gaaaccaagc caccacagcc ttataccgaa
    36541 ggtacgttga tcacggcgat gaaaacagct gggaaaaccg ttgatagtga agaagcgcaa
    36601 tccatattga aagaagtgga aggaatcggt acagaagcga cacgggcgaa tattattgaa
    36661 accttgaaac aaaaggaata tatcaaggta gagaaaaata agctcgtggt gacgaataaa
    36721 gggattttgc tttgccaagc cgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc
    36781 gccaaatggg aaagctactt gttaaagatc ggtgaacgaa aaggaacaca aaccactttt
    36841 ctagccaata tccaaaaatt tgtctctcac ttattggaag tcgtaccagg gcaaatccaa
    36901 tccactgatt ttggatcgac gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc
    36961 gcgcgtcatt taggaatttg tccaaaatgc caggaacaag aagtcttact ctatcaaaag
    37021 gtggcagcat gtacgtctga agcctgtgat ttcaaactat ggacaacgat tgccaaaaag
    37081 aaacttacag cgacacagct gaaagagatc attcaaaata gccgaacaag ccaaccagtg
    37141 aaagggttaa aaggtcagaa gggttcgttt gaagcaacga ttgttttaaa agaagacttc
    37201 actacaggct ttgaattttc tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc
    37261 actacaaaat aatggagggt taatcttatg cgtaatacaa cacacaaaca acaagaaatc
    37321 gaaaatcaaa aagccaaaat tcgttcagaa caggaaaaaa tgcgtgccag caaacgaaga
    37381 ctgcagcaat tgaagaacca gcagcggatt caaaaagaaa tcgaattaga tcgataaatt
    37441 cctttcttgt taatataaaa ctaacatgtt atagttgtgt tagttttata ttaactttag
    37501 gggaagagta atgaaagaca aaatccaatg tatttttgat aaacataatg gcaacctatc
    37561 cttgaaggat gctagaagtg aaggattatc gcctatgacg ttagatcgat tagccgaaaa
    37621 ggataaaata gaaaaggtag caccaggttt ctatacctta aaggatgcgt tcgtggatga
    37681 attgtatctc gctcagagta tctattccag agggattttt tctcatgaga ctgcgctcga
    37741 tctttttcaa gtaggaacat atatccctaa aaagatcaat atgatgtttc ctaaaggata
    37801 caatgtatca aaggagaaat tagatcagta tgccattcaa ccgcattaca ctaataaaga
    37861 aaattttgac ctgggagtca cagaaacaga aagcttttac ggcaataaaa ttttagtcta
    37921 tgatttagag cgaacgttat gtgacatgtg gaatccaaga tacaaagcaa gcgtagaggt
    37981 aaaacaagaa gcattaaaag aatatatgac aagtaccagt agaaatacaa ataagctaag
    38041 aagatacatg gatattttaa aatcaccaaa agaaatgact ttgtatatgt tgccacttta
    38101 ttgagaggag taaagatgaa tgacaccgac acaatttaaa gagaaaacaa gaaatttcgc
    38161 agcagagaaa catttaaacc cacaaattgt ccaaagatat tatatgatgg aaaaattatt
    38221 agaatgtatt gttgaatcgc cttatcatga tgattttgta ttaaaagggg gatttctgat
    38281 tggatcaaaa tacgggttag aaaaccgttc aaccgttgat attgatacga cttatcgaaa
    38341 ttcgaaacta acaaaagaaa agttagaaac tgttttttct gaattaacag ctaatcctac
    38401 aaaagatgga attcgttttg aattaaaggg attaacagag acgagggaag ccgattatta
    38461 tccaggtttt caagcaaagt ttattgctta tcttgaaaat gcacgtgtac cttttaaatt
    38521 agatattact tcaggtgatt ctattattcc agcagcattg atgtatgaac acaaattaat
    38581 gtttgaagat aaaacgattc atattccagc ttatcctacg gaacaaattc tagctgaaaa
    38641 attacatgcc acgtttagtt ttggaaaaga taattcgcgc atgaaagatt tttatgacct
    38701 gtatatgatt ccgaaattag aacgtatcga tgagaaaaat ttgtataaga gtgttcaagg
    38761 aacattcaag gaaagaaata atcaatcggt gtttaagcat ttgtatgatc aggaaatgcc
    38821 tcaattggtg aaagatgagg gcttgagaga aaagtggaaa agataccaac aagaaaacta
    38881 ttttgccaaa gacatttctt tcgaatctac actaacttcc gttgattccc tgatgcaatc
    38941 aattgtagag caagagaagg cagaacgaaa acaaaatttt aagagatcac aacaaatgaa
    39001 tcaagaaatg gagagataaa gatgatcaat aatgtaactt tagtagggcg attaactaaa
    39061 gatccagatt tacgttatac cgcaagtggc acagcagtgg cgactttttc cctagccgtt
    39121 aatcgtaatt tcacgaatca aaacggcaat cgagaagctg attttatcaa ttgcgtgatt
    39181 tggcgcaaac cagcagaaac gatggcgaca ttagctaaaa aaggcagctt gatcggcgtt
    39241 gtcggacgaa ttcaaacacg gacttatgac aaccagcaag gacaacgtgt gtatgtgaca
    39301 gaagttgtgg ccgataactt ccaactatta gaaagtaaag cagcgacaga aagccgtatg
    39361 cacgctgatc aatcgtccag cagtccaagc cctaccacct ttgaacaacg ggatacagcg
    39421 acgcctaaca acaatggatt gaacgcttca cagaatccat ttggtggaca atcaatcgat
    39481 attagcgatg atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt
    39541 ttatgttata atttagacaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg
    39601 gcgggctagt ggctttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc
    39661 tttcttggca aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat
    39721 gattgcaaaa acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat
    39781 taggccgggg tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg
    39841 atttttgcta taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata
    39901 aaatccgagt aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg
    39961 aatcgattaa ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg
    40021 atggaaaata caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg
    40081 ctagcatgta tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaataaaag
    40141 aagagttgta gtcctaaatg aggattgcag ctctttttgt tttacataga aacaggaaaa
    40201 gaccttttta ggaagacaca agtgccttct caattgaaaa acctttctct cactccaacc
    40261 gccaccgccg ttcgagaaga aaggaagtga acgctgcacg ttcactaaga tatggtgtct
    40321 tgcaaagcaa gacaaaatag acaaaacttc ctctcaccaa aaggttcgtt cctgttcgtt
    40381 atctctttat tgatttgcca gaccggcaac aagttgcctt ttatcaccaa aaagcgacaa
    40441 ggacaagggt aaatggcacg ctgcgcgccc ttgtccttgt cgcttttcgt tgatttattc
    40501 tggccaacaa atcaataaag aaagaactaa cgaaaggaag taaaaccttt cctaagagga
    40561 ggcagaatca aacatgcgaa agaaatttga ttattggggt gttcccttca gcgaattatt
    40621 cccaaactat catgcaccac atactgttga atgcgattgt ggagaaagag cgaaatgtat
    40681 caaaagctat cgtttgtatc aatgtccaac atgcgggaaa aaatatacct tgtcttatgg
    40741 cgattacatt ttgattgatg agaaaggaaa gaaacgctga tgaaaacaca acacaaatta
    40801 ttgactgtaa aagaagtcgt tcaaattttg gtggacaaag aagtcatttc tgaaaaagta
    40861 gcagaagctt tacttattcc gcggtactgg acgatagaaa cccctttagt agaggatgca
    40921 ccgactatgc tatttgaaga cctagtgaag attgcaggat atgaactgca ttatcaagaa
    40981 aacatggaaa cttatgaaat cgaatattgg ttaaccaaat tagagaacta aaaaggagaa
    41041 aaaatatggc tcaaaattac tatgacgaat ttgtcaaatt acctttggat aaaatggcac
    41101 aaaaaatgga ggatatgacg ttcctatacc atgaaacacg tgtacccaaa aaacattaca
    41161 aggaaaaatt gtcagtcgca gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca
    41221 ttgcgacgta ctatcgaaca ctggaagaac tgaaaaaaca aaatggaaaa tggtttttcc
    41281 aagccttact atgcttggaa gtaggtgtca aaccaagcac tatcaaacct tccgaatatc
    41341 aagccttaga actgacttat gcaaaattcg cacagattaa gaaagctaaa gcaatggcta
    41401 gcgactggct agatactttt gaaagtatcg aaaaaaatgg tgcattttat acgattgaat
    41461 cggaggagaa agataatgaa taaggtggtt tatcagctaa caacagaaga tgtttcgcaa
    41521 tttttaacaa gcaaagaaca cttgaatgaa gtaatcagac ggattcaaaa agactttagt
    41581 tgtatggaag aaatgaagta ctttattcaa agagtggaag aacagttgta tatggaaagc
    41641 gaaacaagtc aaacgaaggt cacagtcagc cataatcatt tagacgtgta taaacgtcgt
    41701 cctgaaacgg taaaaaaagg aacagagctt ctacgactag aaacagaaaa atacatcatg
    41761 aatgtgtacc tagaaaggag tgagctggtc gaaaactttg tcagctacac gatttgtagg
    41821 aaacactatt actcagaatc agaatattat tcaattttcg agagcatgga attaatggaa
    41881 gaagaaatgg aagagctaaa cagcaaaaat gcgacggtag acgattatta ccttgtattt
    41941 actaggtttt tgacgtgtgg gacggaatct atcgtgttaa tgggaatgga ttatgaactc
    42001 gctgtacccg aagcattttt gagggaatat gtagaagaag tagataaccc aggcgacgac
    42061 tggtacacga ctttaactga ttttttagac acctacactt acgacgatac agaagaaatc
    42121 caaaatgaac ttgaccgtaa acgaatgcca tatcaacgat ttgaaaggaa aaaaggtaaa
    42181 taaccattta taccgaaaag tgaggatgtg tgaaataaga acaaaaaacc ttgaaattaa
    42241 gggaatggtt cttatttcgc accaatcgct ccaaccgccc ccgccgctta ggtggtgcga
    42301 aataagcggg caaagcccga taacctaaga gaaagaagga gatccatatg aataaaatca
    42361 ttcctctatt gttcgtactg tttgttggcg agatacttat gcgtcatgct gcagctgtac
    42421 atttaaactt cgtagtattc ttaggaatac tggtattttg tttgatcttt tggcgatttg
    42481 tattagccct tgtgagtcta ctattcaaga tgattgcagc catatttttc atagggttat
    42541 ttcgatgaaa ggagagagta ctaatgccaa ttgcaatagg aaataaacgg ttacccgtga
    42601 cattagatga aaagagacaa aaagaattgc agcaactaaa gcagaagtac ggaaaaagtg
    42661 aatctaagat tatgtgtatt gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg
    42721 atataccagc actaaaaaag tgacgtcact tttcatttga agaaacaaaa gtgatagcac
    42781 ttaattgttc ctactattta gaatattttc tatgtccttt ttattccatt ttgtatcaat
    42841 aactatgaaa tctttaaagt atccagaaga gattattttt agatcaattt ttttcttacc
    42901 tgattctaaa gattttgttt tcttaaacat attatgattt cctttcgact attttcatat
    42961 aaatgttaac atagtttatg gattcatgga atgttaatct aatttaatag aaaaggataa
    43021 ttaagtatat acgttttttc ttatgtatgt tatactatgt atatacagaa agaggtgtct
    43081 actatggaaa tgttacctat aaaaaaatgg ggaaatagta atggccttag attgccaaag
    43141 tatattatgg aatatcttgg aattcataca gaagacaaag tgaagataat acaagaagaa
    43201 gtaaacggtc agaagcggct aattatcgaa gcggctaata tagaaaatga attaacaatt
    43261 gaacagttat ttgaaaatta cactgatgaa agaaatcatg tgacgattca aaatttaggt
    43321 gaagcagtag gtaatgaaaa atggtgagaa aacctagaca aggcgatatt ctattattga
    43381 atacagcacc tcgatctgga cacgaacaga caggtaaaag accttatatt gttttaagtc
    43441 atgatattat tgcggattat agtaacgttg ttacagttgc tccaatttct agtacaacta
    43501 gaaactatcc gctatatgta aatataaact cagaatatga aatgaagaca acaggaaaag
    43561 tattattaga tcaactaacg acaattgatt atgaagccag agagtgtatt tttttggaaa
    43621 aagcgcatga tagtttagtt gaggagttat tgatgaaagt tagaacagtt tttcaaaaag
    43681 taagcaaata aaatagaaac agcagcatga tcaactattc gtgattgacc atgctgtctt
    43741 tttttattct atatacggtt tgtctcgtaa cattgtagtc ttttgcaatc ttagcgatcg
    43801 caacaccttt ttttagatcc tcaacgatgt ttttataaac aagacgacgt tggggatctt
    43861 ttgcattggc actgtatagc ttaggacgtc ctttataaac accgttcgct tttgcaatct
    43921 taatgccttg tgcctgacgt cgtttagatt cggtacgttc ttgttcagcg atcatggcca
    43981 aaatttgaat gatcaaatct ttaatgaatt tatccaggag aggattgcca attgcttcgg
    44041 ccatgatcgg tagacttgtg atgatcagct gcacattttt cttcttcaag taattgactg
    44101 aagcgatgat ctcgtcgtaa tttcttccaa gtcgatcaat agcttccaca ataaaaatat
    44161 cttgatctcg gacgaaatcc agtgcttctt gaaaaacaag tcgatgagta atcgttgcgc
    44221 cagattgttt ctcaatgaaa attctctctg caccgaattt tttcatttct tcaatctgcc
    44281 gttgttcatt ttgatcgatg gatgagacac gtatgtatgc aactctcaaa atttttacct
    44341 actttctgca actatttaaa aaacacccta atcatacacg agaaaaccag atttttcaat
    44401 gtcgcggtcg tgtctagtta gggtatacct tatatagaca aaaactaatg atggaggaaa
    44461 tagtatgtgt cacttttctt tttaaatggc aatatttttt gtaaataaat gtcaatcaag
    44521 aaaatttaaa cgattatgac attaaaaata tattttatga attttctagt atataacttg
    44581 atttatctgt taagatttat taagaaaacg ttttcttatt tttgatttcg aggtgataga
    44641 caatgaaaaa taagtttatt ttatctttag gtattttaac tattggaatt agtgtaactg
    44701 gaactacaat tcctacattt gctgatagta caaatgaacc aactactgta gtagataata
    44761 accaagaatt aactccttta gataggaacg gaatagccat tggtaaccca actaacgact
    44821 taactgttac tgaaaattat ttaatagttt ctccattact tgctcgagca gcttctaaaa
    44881 cgatacgagt gacaggaacg atgatttttg ataaagttgg ttggccttct agacctcctc
    44941 aatcacggaa ttggaaagaa aaaagaaatg gttattggta ctatggaaaa ttgtatttac
    45001 aaagttataa atcagctgga aacaattgga ttgcgactta ttcaggaacc ttaactagaa
    45061 agtagggatt ttttatgcca gaatttgtga acacaccaaa tttttatcaa gatagcgata
    45121 ttctaagtaa aattaattta ccgaaagaag aatcagcttt acaagttcag cgaactttac
    45181 aggagacaca aaaaattcaa aaatcaattt ctaatctaga agattctgaa gaaaaaagaa
    45241 aaaaaagaga ccaaaaaaca tatcaaaaag aaaaataatg attttaaaaa aagagtgtct
    45301 agtcgacatt ctttttttct aattgaaagt gttcataaaa tcacccattt ctggacactt
    45361 tctttttttg ctttattttt caaaaaaaca attcatttca tgttcataaa cccataacaa
    45421 tatctatgtt cagttaaact atatggataa agttatgtta caataaaatt aatgaaggag
    45481 tgattttttt gaaaatagga tacgcccggg tatctactgg tttacaaaat ttggatttac
    45541 aagaagacag cctaaataaa tttggttgtg aaaaaatttt tactgatcat atgagtgggg
    45601 caaaaagtaa tcgaccagga ttagaaatgg ccattgattt tgttcgctct ggcgatacac
    45661 ttgtcgtttg gcgcttagat cgtttaggta gaaatatgga agatttgatt tcaatcgtca
    45721 atcgactgaa tgagcgtggt gtgagttttc atagtattca agaaaatatt acgatggaca
    45781 aatcaagttc tacaggacag ctcatgtttc atttgtttgc ggcatttgca gaatttgagc
    45841 gtaatttgat cctggaacga tccgcagcag gacgagcagc tgctcgtgca agaggacggt
    45901 ttggtggtcg tcctgaaaaa ttgtctgaac aagatttgga attactgaag actttagtgg
    45961 atagcggtac acctattaag actattgcgg aacggtggaa cgtctcaaga accaccattt
    46021 atcgttattt ggcaaaaatg gaggagaaaa atggaaagac aaattctcat tagtttttgt
    46081 catttaatgc tgaaaatttt tgccatttat ctagaaaagt gacaatagta tgagaatttt
    46141 ttgtaagtgt ctactgataa cttattctca aatgttgtaa ggattttatt catttgttaa
    46201 tatta
//
LOCUS       DAHUVV010000014        40584 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.014, whole genome shotgun sequence.
ACCESSION   DAHUVV010000014 DAHUVV010000000
VERSION     DAHUVV010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.014"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            666..2276
                     /locus_tag="NK767_000760"
     CDS             666..2276
                     /locus_tag="NK767_000760"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCH6693773.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2568..3437
                     /locus_tag="NK767_000761"
     CDS             2568..3437
                     /locus_tag="NK767_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCH6693774.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3602..4873
                     /locus_tag="NK767_000762"
     CDS             3602..4873
                     /locus_tag="NK767_000762"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCH6693775.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            4870..6165
                     /gene="rho"
                     /locus_tag="NK767_000763"
     CDS             4870..6165
                     /gene="rho"
                     /locus_tag="NK767_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCH6693776.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            6264..6527
                     /locus_tag="NK767_000764"
     CDS             6264..6527
                     /locus_tag="NK767_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCH6693777.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            6972..7421
                     /locus_tag="NK767_000765"
     CDS             6972..7421
                     /locus_tag="NK767_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCH6693778.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7478..8896
                     /locus_tag="NK767_000766"
     CDS             7478..8896
                     /locus_tag="NK767_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCH6693779.1"
                     /translation="MKIVGVTACPTGIAHTYMSVEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            8896..9756
                     /locus_tag="NK767_000767"
     CDS             8896..9756
                     /locus_tag="NK767_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCH6693780.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            9930..10703
                     /locus_tag="NK767_000768"
     CDS             9930..10703
                     /locus_tag="NK767_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCH6693781.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            10850..11215
                     /locus_tag="NK767_000769"
     CDS             10850..11215
                     /locus_tag="NK767_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HCH6693782.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(11363..12571)
                     /locus_tag="NK767_000770"
     CDS             complement(11363..12571)
                     /locus_tag="NK767_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6693783.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(12590..12868)
                     /locus_tag="NK767_000771"
     CDS             complement(12590..12868)
                     /locus_tag="NK767_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693784.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            13022..13717
                     /locus_tag="NK767_000772"
     CDS             13022..13717
                     /locus_tag="NK767_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCH6693785.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(14159..14956)
                     /locus_tag="NK767_000773"
     CDS             complement(14159..14956)
                     /locus_tag="NK767_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCH6693786.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(14953..16137)
                     /locus_tag="NK767_000774"
     CDS             complement(14953..16137)
                     /locus_tag="NK767_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCH6693787.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(16162..17409)
                     /locus_tag="NK767_000775"
     CDS             complement(16162..17409)
                     /locus_tag="NK767_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCH6693788.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            17624..19369
                     /gene="dnaX"
                     /locus_tag="NK767_000776"
     CDS             17624..19369
                     /gene="dnaX"
                     /locus_tag="NK767_000776"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCH6693789.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            19389..19703
                     /locus_tag="NK767_000777"
     CDS             19389..19703
                     /locus_tag="NK767_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCH6693790.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            19728..20324
                     /gene="recR"
                     /locus_tag="NK767_000778"
     CDS             19728..20324
                     /gene="recR"
                     /locus_tag="NK767_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCH6693791.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            20388..21032
                     /locus_tag="NK767_000779"
     CDS             20388..21032
                     /locus_tag="NK767_000779"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCH6693792.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            21047..21376
                     /locus_tag="NK767_000780"
     CDS             21047..21376
                     /locus_tag="NK767_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCH6693793.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            21390..22328
                     /gene="holB"
                     /locus_tag="NK767_000781"
     CDS             21390..22328
                     /gene="holB"
                     /locus_tag="NK767_000781"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCH6693794.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            22364..23188
                     /locus_tag="NK767_000782"
     CDS             22364..23188
                     /locus_tag="NK767_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCH6693795.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            23181..23528
                     /locus_tag="NK767_000783"
     CDS             23181..23528
                     /locus_tag="NK767_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCH6693796.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            23597..24469
                     /gene="rsmI"
                     /locus_tag="NK767_000784"
     CDS             23597..24469
                     /gene="rsmI"
                     /locus_tag="NK767_000784"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCH6693797.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24578..25699
                     /gene="dinB"
                     /locus_tag="NK767_000785"
     CDS             24578..25699
                     /gene="dinB"
                     /locus_tag="NK767_000785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCH6693798.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(25753..26355)
                     /gene="nrdG"
                     /locus_tag="NK767_000786"
     CDS             complement(25753..26355)
                     /gene="nrdG"
                     /locus_tag="NK767_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCH6693799.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(26670..28859)
                     /gene="nrdD"
                     /locus_tag="NK767_000787"
     CDS             complement(26670..28859)
                     /gene="nrdD"
                     /locus_tag="NK767_000787"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCH6693800.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            29158..29952
                     /locus_tag="NK767_000788"
     CDS             29158..29952
                     /locus_tag="NK767_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6693801.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            29942..31984
                     /locus_tag="NK767_000789"
     CDS             29942..31984
                     /locus_tag="NK767_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6693802.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            32265..32414
                     /locus_tag="NK767_000790"
     CDS             32265..32414
                     /locus_tag="NK767_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCH6693803.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            32425..33939
                     /gene="dltA"
                     /locus_tag="NK767_000791"
     CDS             32425..33939
                     /gene="dltA"
                     /locus_tag="NK767_000791"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCH6693804.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            33936..35141
                     /gene="dltB"
                     /locus_tag="NK767_000792"
     CDS             33936..35141
                     /gene="dltB"
                     /locus_tag="NK767_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HCH6693805.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            35186..35419
                     /gene="dltC"
                     /locus_tag="NK767_000793"
     CDS             35186..35419
                     /gene="dltC"
                     /locus_tag="NK767_000793"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HCH6693806.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            35422..36690
                     /gene="dltD"
                     /locus_tag="NK767_000794"
     CDS             35422..36690
                     /gene="dltD"
                     /locus_tag="NK767_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCH6693807.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            36831..37913
                     /locus_tag="NK767_000795"
     CDS             36831..37913
                     /locus_tag="NK767_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCH6693808.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            37913..38716
                     /locus_tag="NK767_000796"
     CDS             37913..38716
                     /locus_tag="NK767_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCH6693809.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            38786..39526
                     /locus_tag="NK767_000797"
     CDS             38786..39526
                     /locus_tag="NK767_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCH6693810.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            39537..40541
                     /locus_tag="NK767_000798"
     CDS             39537..40541
                     /locus_tag="NK767_000798"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCH6693811.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT        13042 a         7107 c         8458 g        11977 t
ORIGIN      
        1 ttttgtcgca tctccttatt ttttacttat gtgattcgtt accttcaatg aatccctaca
       61 aggcactttt gttgaaaaaa aggtcatctt tgaaaaaatt agtggaattg ttttgttttt
      121 gatattattt aatagaaaag atatgccaaa aatccgagaa ctgtggacta cgttttttac
      181 gtaaaggagg taaataaggg tgaaagaaaa agattgtttc tacgataagc tatataaact
      241 gttgctgcaa gtaggaaaaa gtagttcttt gttaggtggg atattatcta tcataatgtt
      301 tgttttattt ttttatctat tatttcactt aaaatttttc agtaacgatt ttctgatttg
      361 cttaggttta acgttagcta tgtttatatc aagcttatta tgtgggttaa attttttttg
      421 cactcataaa ggacttaaga aataatttct caaaggatat cgaaaaagaa aaaattgtaa
      481 gcggatagta ttcatttgag tacttgactt tcccttagtc agcttgtatt atacaccttg
      541 ggcacccttt tagagggtca gaagtttaca gaatttcggc tccctattct tattgaagaa
      601 tagggagctt tgtttattgt ctgagacttt tcctgcacat aaattaaaaa ggagtttttg
      661 aacttatgac aaaatatatt tttgtgaccg gcggtgtcgt ttcatcaatc ggaaaaggga
      721 ttgttgcagc ttctttagga cgcttattga aaaaccgcgg cttaaaagta actatccaaa
      781 aatttgatcc atacatcaac gtggatccgg ggacgatgag cccatatcag cacggagaag
      841 tattcgtaac agatgatggt gctgaaacag atttggatct gggccactac gaacgtttta
      901 ttgacatcaa tttgaacaaa tactccaacg tgacgacagg aaaaatctat tctgaagttt
      961 tgcgtaaaga acgtaagggc gaatacttgg gagcaactgt tcaagttatt cctcatatta
     1021 caaatgagat caaagaaaaa atcatgcgag cagctaaaat gactgattca gatgtgatca
     1081 ttacagaagt agggggaaca gtaggggata tagaatcttt gccattcctt gaagcacttc
     1141 gtcaaatgaa agcagatgtc ggtacagata atgttatgta tatccataca acattgattc
     1201 cttacttgaa agctgctgga gaaatgaaaa ctaaaccaac gcagcacagt gtaaaagagc
     1261 tacgtggttt aggtatccaa ccgaatattt tggtggtacg tacagagcaa cctgtttctc
     1321 aaagcgtgaa aaacaaattg gcacaatttt gtgatgtcga accagaagca gttatcgagt
     1381 cgccagatgt agatacactt tactccatcc cattagtttt acaagcacaa ggaatggatc
     1441 aaattgtttg cgaccattta aaactagata caccagaagc agacatgaca gaatggaaag
     1501 aattagaaga acgtgtcttg aacttgaaga aaaaagtacg gattgcgctt gtcggtaaat
     1561 atgtcgagtt accagatgca tatatctcag tagttgaagc attgaaacat tctggtttcg
     1621 cctataattc agacatcgag atcgattgga tcaaagcaca agaattaacg agagaaaatg
     1681 tagaagaacg tttgaaagac gcagatggaa tcttggttcc tggtggattt ggtgatcgcg
     1741 gagtagaagg gaaaatcgaa gcgattcgtt atgctcgcga aaatgacgtt ccgtttttag
     1801 gtatttgtct tggtatgcag atggcttgtg tcgaatttgc acgtaacgtc gttggattag
     1861 aagatgcgtc atcagctgaa acgaatccag atacagcaaa caatattatt gatttaatgg
     1921 ctgatcagga aaacatcgag aatttgggtg gaactttacg ccttggatta tacccatgta
     1981 aaatcaaaaa aggaacgaag acggctgaag catatgatgg agccgatgtt gtgcaagaac
     2041 gtcaccgtca ccgctatgag ttcaataaca aatatcgtca attgtttgaa gaacaagggc
     2101 tagtcttttc aggtgtctca ccagataacc gtttagtaga aatcgtagag ctgccagaga
     2161 aaaaattctt tgtgggatgc caattccacc cagaattgat ttctcgtcca aatcgcccgc
     2221 aaaaattaat caaagcattt gtcggagcat cattagcaga tcacgaaaca aaataatttt
     2281 tgaacttgat agaaagagaa aagtagcaag agaaggggat aagcaatttt cttgtcactt
     2341 ttctcttttt ctgatttgtt attatttgta ggaaaaaaga tagaaagcaa aagacttttt
     2401 tcgattaaag cgctattgga tattttattt tttgcaaaaa acttcttgaa aattttcatt
     2461 ttcattgatt ttagtagaaa actaccgaaa tgtttggtaa aatagggtcg ttggttaaga
     2521 attgtcttaa caaatttagt tttacaaaac ttaggaggaa ttttattatg ccagtagtat
     2581 caggagctga atttttaaaa gctgctcgta aaggcggtta cgctgtcggc ggattcaaca
     2641 caaacaactt ggaatggact caagcaatcc ttgaagcggc tgaagcaaaa caagcaccag
     2701 tacttatcca aacatcaatg ggtgcagcta aatacatggg tggttacaaa atctgtaaag
     2761 atttaatcac tgatttagtt gaagcaatga acatcactgt tccagttgct atccacttag
     2821 accatggtga ttacgaagct gctttggaat gtatcgaatt aggctatact tcagtaatgt
     2881 tcgatggttc acatctacca tttgaagaaa acttgaaatt agctaaagac gttgttgaaa
     2941 aagcacatgc aaaaggtatc tcagttgaat gtgaagttgg ttcaatcggc ggagaagaag
     3001 acggaatcat cggtaacggc gaattagctg atattgaaga atgcaaacaa atggttgcta
     3061 caggtatcga ctacttagct tgcggtatcg gtaacatcca tggtcaatat ccagaaaact
     3121 ggaaaggatt agcttttgac cacttacaag caattgctga tgctgtaggt ccagacatgc
     3181 ctttagtatt acatggcgga tcaggtattc ctcaagatca aatcgaaaaa gctatctcaa
     3241 tgggtatctc aaaagtaaat gttaacactg aattccaatt atcatttgct gcagctactc
     3301 gtaaatatat cgaagctggt aaagataaag aaggtaaagg attcgaccct cgtaaattat
     3361 tagctccagg taaagcagca atcgttgaag atgctaaatc tcatatcgac tggttcggtt
     3421 cagctaacaa agcgtaagca tccacataac agatacgata tataggaatg gagaacgact
     3481 tttgaagtcg ctctccattc ttttttactt aaaacggcag accatgctcg tttctttttt
     3541 ttatctatga taaaatacaa gcatatccat gaaaaaagat taataaacaa ggtggaaaca
     3601 gatgaaaaaa atcgtaatca aaggaaaccg acctttagct ggtgaagtaa cgatcagcgg
     3661 tgcaaaaaat agtgcagtcg cattgattcc agctgcaatc ttagctgatt ccccagtgat
     3721 cttggaaggt gtgccggaca tacaagatgt tcattcatta attgaaattt tagagatcat
     3781 gggcgcaact gtccattttt ccaacaacat actagaaatc gatcctcgag aagtcgtatc
     3841 gattccgatg ccgcacggaa aaatcaatag tttgcgtgct tcttattatt ttatgggaac
     3901 actgcttggt aaatttggcg aagcggtagt aggcctgcca ggtggctgtt atttaggccc
     3961 tagaccgatt gacctacacg tcaaaggatt tgaagctcta ggtgctgaag tgacaaatga
     4021 acatggtgcc atgtatttac ggacagaaaa taaaggattg cgtggcaatc ggattttcat
     4081 ggacgtggtt tcagtcggag caacgatcaa tgtgatgtta gctgctgtaa aagcaaaagg
     4141 acagacagta attgaaaacg ctgcacgaga accggaaatc atagatgtgg caactttact
     4201 gaataatatg ggggcaaaag ttcgaggagc gggtacagat gtgatccgta tcgaaggagt
     4261 cgaaacatta catggctgtc ggcatttcat gattcctgac cgcatcgaag caggaaccta
     4321 tctagcatta gcagctgcag taggaaatgg tatcaaagtg aaaaatgtga tctttgaaca
     4381 tttggaaagc tttatagcaa aattgcagga aattggtgtc catatgaaaa tcagcgaaga
     4441 tgaaatcgaa gtttacccct cgaaagaatt aaaacccgca aacatcatga cctatcctta
     4501 tccaggattt gccacagatc tgcagcagcc acttactgcg ttactattga tgactacagg
     4561 cacttcagaa atcatcgata cgatctatgc gaaacgtgtg aaccatgtgc cggaactagc
     4621 acgaatgggc gctgatatta ccgtggaagg aaatatgatc atcgtgaatg gtccaaataa
     4681 acttcacggc acagaagtcg tggcttctga tcttcgggca ggtgcttgtt tagtgattgc
     4741 tggactattg gcagaaggga caacgacgat ttacaatgta gattatatct tacgcggtta
     4801 tgatcatatt atcgagaaac taacagcctt aggggcatca attgaaatga tagaggaagt
     4861 gggagctgaa tgagcgatta tttgacaatg gcagaactag agaacaaaac gttaaaagaa
     4921 gtttatagtt atgccaaaga atttaaaatt ccttattaca gtaagatgaa taaaaaggaa
     4981 ttatctctag ctgtgattcg tgcacaagca gagaaacaag gattttatta tatggaagga
     5041 atattggata tcgttggtca agatggttac ggttttttac ggccaatcaa ttatggacca
     5101 agtgcagaag atatctatat ttctgcctca caaatcagac gattcagttt gagaaatggg
     5161 gacaaagtgg caggaaaagc ccgtccacct aaagaatcgg aacgttatta tggtttgatg
     5221 cacgtggaaa gcgttaatgg aaaagatccg gaagaagcaa aagaacgtcc gcattttcca
     5281 gccttaacac ctctttatcc gcaaagacag cttcaactgg aaacaacacc gcaaagacta
     5341 tctacccgaa tgatcgatat cttttctcca atcggttttg gtcagcgtgg attgatcgtc
     5401 gcaccgccaa aagcagggaa aacttctgta ttaaaagaaa tcgcaaatgg tatcaccgaa
     5461 aactatccgg aagtagaatt aatcgttcta ctgattgatg aacgaccgga agaagtaacg
     5521 gatctagaac gaagcgtgaa aggcgacgtg gtatcttcta catttgatct gcagccgcaa
     5581 aaccatactc gagtttctga attagtattg gaacgcgcaa tgcgtttagt cgaagataaa
     5641 cgagatgtcg ttatcttgat ggatagtatt actagacttg ctcgggccta taacttagtc
     5701 gttccaccaa gcggacgtac attgagtggc gggattgacc cagccgcatt gtataaacct
     5761 aagaaatttt tcggggcagc aagaaacatc gaagaaggtg gcagtttgac gattttagcg
     5821 accgctttag tcgatacagg aagccgtatg gatgacgtga tctatgaaga attcaaagga
     5881 actggaaatc tagaattaca actttcacgt gaattatctg aacgtcggat cttcccagcc
     5941 atcgatatca aaaaatcagg tacccgaaaa gaagaattat tgatgccaag tgaagaatta
     6001 gaagaaattt ggaaacttcg aaaaaatatg actggagatt cattagaata tacggaacaa
     6061 ctgatccgct ttttgcgtaa aacaaaaaac aatcagcaat ttttcaaaga atttcaagat
     6121 gtctcctttg gtaaaaaaaa tccgaccaga aatctgaaaa gataattgca accttgactg
     6181 aaacatgtta agatgttaat gttgtaaatc gacaaatcaa ctctggttca gcaaatggag
     6241 cagggcaaag gagcgaaaaa atcatgaaag aaaacatcca tccagattac cacccagtag
     6301 tattcatgga ttcaacaact ggtttcaaat tcttgtcagg ttcaacaaaa acttcacaag
     6361 aaacagttga atgggaagac ggtaatacat acccagtcat ccgtgtcgaa gtaacttctg
     6421 actcacatcc attctacact ggacgtcaaa aattcacaca agcagacgga cgtgtggacc
     6481 gtttcaacaa aaaatacggt ctcaaagacg caaacgctgc ggaataataa aaacgctgat
     6541 aactcaacgt ttagcagact atctgatagg gatagtctgt ttttttatgt tttttgccaa
     6601 tttaaaactt cgtttgatgc tgaaaaagtc gtaggatcat aatgcacaaa acaatgaaaa
     6661 actagtatat agttttaatt atccacttga taaaaacacc ttatattaac gaaatgaaga
     6721 ggggttattt cgaagtaaaa aaccatttac ctaaaattag aagctaagct ttcctttcaa
     6781 ggaaggcttt ttgttttgtt atagacttgc gattgtttgt gagctagatc gttcatttta
     6841 atgacttata cacgcggatg atgggtcttt ttcctctgtt cattcataat attgaacggt
     6901 acagctagcg atgctatact gacctcagtt ccaagaaaac gttaacattt tttgtggggg
     6961 agaggttttt tatgaggttt gatcaattga tcgcagaaga actgattgtt ttcgatccat
     7021 cgatagattc aaaaggatca ttatttgagc gaatagggga tgtgttgaaa aaaacaaata
     7081 aggtaagaaa tgttcaaaaa gtcgttaagg cactattcaa acgagaggag gagatctcca
     7141 caggaataga agatggattt gggattcccc atgcaaaaag caaatatgtg acaaaaccag
     7201 cactgtgttt ttttcataca gccaaattaa cggactatag cgggatagat gaaatgccaa
     7261 ttgaatatat ttttgccatc gtagttcctg aaaaatcagc agatatgcat ctagagattc
     7321 tcagcacact ctccagacga ttgatgaatg cagattttag aaagggaata aaagaagcca
     7381 ctacgccagc agatatcatc agacagatta gtcaagaatg aaaatagtag agtctttgtc
     7441 cgagtatcga tagtgaagaa aaaggaggaa aataatcatg aaaatcgttg gtgtaactgc
     7501 ttgcccgact ggaattgctc atacttatat gtcggtagaa aaattagaag taacagcaag
     7561 aaatctaaac catcaagcaa aattcgaaac acaaggtgta aaaacagaaa atcagttatc
     7621 tgaacaagaa atcaaagagg cagatgcaat cattcttgca gtagataaag aaatcgaatt
     7681 ggaccgattt gccgggaaaa aagtaaagcg tgtctctaca agccgagcaa tcaaagaacc
     7741 gcaagtagta atagacgaag cattgagaga tattggcgta ttcgttgtgt ctaatgaaaa
     7801 agaaccagct gcaaatgaga aaaagccgac aatctataat catttcatga atggtgtgaa
     7861 ttacatgctt ccatttgtga tcgcaggcgg aatcatcatc gcactgagtt ttgcctttgg
     7921 gattacagcg gctgatccca actcagcaga ctataatgtg ttagcagctg cattttcaag
     7981 gatcggagga gatacagcgt ttgccatgat ggttcctgcg ttaggcgcgg ggattgctac
     8041 atcgattgct ggaaaagcag gttttgcccc aggaattgtt gccggattac tggcctctac
     8101 aggtggttca ggttttcttg gcggaatgat tggtggcgtg ctagcagggt atatctgtga
     8161 ttttctggca aataaaatta gcgtaaaaaa agaattttct gctatttatc agctgattgt
     8221 cgtccccttt ttatcgatct taattattgg gcttttgatg gtatttgtga ttgaacaacc
     8281 gatcacatgg gtgttggatg ctttgacaaa ttggctgaat agcttgggaa atacatccgg
     8341 gctattgttc ggattactta tcgggatcat gatggcagct gatatgggtg ggccgatcaa
     8401 taaatcgact tcgactttct cgatcggttt gatgtcagct ggagtaaatg caccgattgc
     8461 tgcttgtatg gctgcaggaa tgacacctcc cctaggtatt gctttagcca ctttattatt
     8521 caaacataaa tttaccaaag aagaacggac ttcaggtcaa tcatgctgga ttttaggcgc
     8581 ttcgtatatt acagaaggtg ctataccgtt tgcggtatct gacccgatcc gcgtgatacc
     8641 tagtttgatg acaggttcag cagtcgctgc agggatttcc atggcagtag gagttacttc
     8701 aatggcacct catggaggga tttgggtgat gctaattcca aatgttatga acaatttacc
     8761 tatgtatgtc ttggcaattg catcaggtac agtcactaca gcctttatgc ttggactatt
     8821 gaaaaaaaca gtgaaagaga aaaaacaaga caagtggacg aaaaatgaaa atctaacaaa
     8881 tgaggtggaa gcgtaatgtt atatacaatg aaagagttat tgaaagtggc taaagagaat
     8941 caatttgcag taccagcctt caatatttgc agctatgata tgatgaaagc aatcatcgaa
     9001 gaagcagaga gattagaagc accagtaatc ttagaaattc atcctgatga aatcgcctat
     9061 ttaagtgatg agtttgttgc ggcagtaaga gcctatgctt atacaagtaa actgccgatc
     9121 gttatccact tggatcatgg cggtacgata gaagatgtct tacgggcgat tcgaaacggc
     9181 tattcttccg tgatgatcga tgcgtctttg aaatcattcg acgacaatgt acagattacg
     9241 aaaaaagtgg ttgcgttagc tcatgcggta aatgtctcag tggaggctga gcttggaacg
     9301 attgggaata atggttctgc tgaaggaggt gctgaaacaa tcatttatac agatcctgat
     9361 caagcgaaga tttttgtgaa agaaacagga atcgatacac ttgctgtagc aattggaaca
     9421 gcgcatggct tgtatccgaa aaacagaact ccaaaattaa atatcgactt actgaaagaa
     9481 ttgaaccaac aaatcgatct tccatttgtt ttacatggcg gttcaggtaa tccggacgca
     9541 gaagtcagcg aagcagtaaa atacggagtt gccaaagtga atttgagctc agatcttaaa
     9601 agcgtctttt tcaatgcttt gagagaaata ttgagtgaaa atccatcgat gtacgagccg
     9661 aatgcaattt atccagcagc taataaaaag gtacaagaag tggtcaaaca taaaatggaa
     9721 attttgaaca cagtaggcaa ggctagttta tactaaaaat aaaaatgtgt ggtcaagagg
     9781 gtatgacaat agtcttgtca taccctctta accacacacg atgttcagaa gtagtttttt
     9841 cgaaaaattt cttcttattt ctcgaagcca atcacttctg tcacaacctc ttttttcctg
     9901 ttactatata aagtaaatgg ggtgatttta tgttgaatga agtggagaat agacagcagg
     9961 agattgtgaa ttatctaaaa aacaaccaat ttgcaagagt caccgatttg atcgagttag
    10021 taaattatag cgaagccacg attaaacgtg accttgtttt tctggaaaaa agtggattga
    10081 tccgtagagt tcgcggtggc gccatgctta ttgacaatcg aaaaattgac gtgccttacc
    10141 tgatgaagat caccaaatta gaagaagaaa cagaaaaacg taagattgca gatacagctt
    10201 cttctttgat tcgagatgat atggtcttat tcttggattc cagtacaacc tctttacact
    10261 tagtcagaaa cctagcaaga tttgatggtt tgcagatcat tacgaatggc atcgtcacag
    10321 ctgctatgtt gagtgaatat acaacggcaa aagtatctgt attgggaggg gcaattgttc
    10381 cgaaacgagc aacaatcaac ggttcaaaag cctataacga tgcattgtca tattatgcgg
    10441 atttagcgat aatgagttgt cgcgggctag atttcagcca aggagtaaca gaaactcatg
    10501 aaggagaggc aatggtcaaa aaagctttta gaaaacaatc agaccggctt gccgttttag
    10561 ttacacagca aaaaattgca cataaattca tgcatcaagc cattgctttt catgaaatcg
    10621 attatctggt gacaagtaaa gaactgacaa aagaacagta tgcgaaagta aaggaacacc
    10681 atatcgaatg tctgtacgga taaattatta cgtgaatgtt ctaagtggct tcctcatttt
    10741 tagataggac cttatctttg ctcgcttctc tcagccgatg gtataataat gaaaaggtta
    10801 tcttgttaga taatcttact tttttatcca ggagaaagga gtgcagaaaa tgaaacatgt
    10861 gaaggcgttg gtagtgaagg caatcatgat ctgggcgatt ctgtgggtcg tgctgacagg
    10921 tttatacggt gttagcttta tggattcaac gattgttggg gtaattattg tagtcatgat
    10981 ctatgtgcta ggggatctgt tgatcttacg taaagtaggg aacatcgcag cgacgatcgc
    11041 agatgctggt tcagctgcag ttatcttgtg gctctatcta tattttatga atgatacagt
    11101 cgatatttgg atgaaagtat tgattcctgc tctattgatc ggtgtggcag aatggttctt
    11161 ccacaagtgg ttgctaagtc aaggcatcgt accagatgaa cggaaaatgg aataaatcaa
    11221 cagttaaact attcttatcg ataaatgaaa gctttaaaaa taaatagatg tttaattaag
    11281 aataatcata aactattttt actcaaaaaa ccgctcagat tattttcaga tctgaacggt
    11341 tttttttatc tattcatcaa gatcaaattt catttttcaa tgattcaatc aattcgccct
    11401 ctgcttcatg actatgtttt cggccattaa caagatagag tgctgtacat atcaatggaa
    11461 tgactccggc aatcacatac aatcctctaa atgaagtaag agattttagc atccccatca
    11521 aataaggacc aacgcctaag cctaggtcta aaccaataaa gtaggtagat aaagcaatac
    11581 tgattcgatg ttcgcggacg atttttaaac aaacagcctg tccatttgac ataaacgtac
    11641 cataacctag gccgactaat gctccagaga taagcaatgt cacactattc gtcgttacac
    11701 tcagtaaaaa taatcctgct gttaagaata aatagctagg atacatcacg tattgttctc
    11761 ctttgacatc gaaaatcctt ccagtcaatg gacgtgtaaa tgtgatgacc aaagcatata
    11821 cgacgaagaa aaatgaccca gcagcaacta agtggattac ttttgtataa gaagataaaa
    11881 atgataaaac acttgaataa gaaagaccca tcaaaaaggc aattcctgta atgaaaagtg
    11941 ctttcttttc aataaaacta ttgatattcc atgagcgaag agatattttt tgttcttcag
    12001 ataatggaat attttttaca ggaaagacaa ggcaggcaat cgtaacaaag aagaccagta
    12061 caattgaaaa ccagataatg aaacgaaaat cagtcatatt cagcaaaatc attcctaaaa
    12121 atggcccaac agcggcagct aaactagtac ttaatccata ataattgatg ccttcccctt
    12181 ttttagatgc agggatatat gcagtaacga tagcatttgt agctgtagaa actgtaccgt
    12241 agccgaatcc attcaagaat cgaactaaat acaatgcccc gatagttggc gtataaagat
    12301 atgctaatgt tgaaaggaga tagaagatcg cgccgcctct taaggtaaac ttccgaccaa
    12361 atagttctaa ctgtttcccc atgaataagc gtgccagcaa tgttccgatg atatagatcc
    12421 cagaagcaaa acctgcttgg ctcaagctgg cgtgtaaggt gtcttgcgca atgactgcca
    12481 tgatgaccat caacagataa taaatcaggt agacaataaa attgatcaat gtgattccaa
    12541 taaaatattt attaaataat ttcttttcca tgtatgttat acattcccct taagctgaat
    12601 atgcttttaa tttttgtatt tgaacttcta catcgtgata attggatgga tagcgatcaa
    12661 caagttggcg aataaataaa tctcttgtag acataaacag atttaaaaat tcttcgcatt
    12721 cgtttgttgg attctcatcc actgctttga tcaattcgat cgagttttga tacgcatcat
    12781 ttaaaaagac taaagaagct ttcatatcaa atcttgtttg atatgctgtt tctaatcgat
    12841 taatgattgt gttcatccag taattcatgt tttttccctc tattcttaaa aaattttact
    12901 tctgcagtat aacgaataaa aaaaaggaaa aaaatcgcat aagtgatgga aaaaggtact
    12961 taacaaatgt taatatcatt agtaaaatac ctttggactt taaaaagttt taaagtactg
    13021 tatgatcttt ttagatgaaa gtttggatgg gggagcatca ttggatagta gagtcttaaa
    13081 ggaatatttg gataaaaatg attttccgat tatacgcaaa ggatatcata aatatttgac
    13141 attcgaaggg atggaagatt cccatacgta tatcttgaaa gagggaacgg taaaagcaag
    13201 tgttatttca aatgatggga gagaatttaa ccttcgctat atcaaagatc ttgaaatcgt
    13261 ctctctttta aaagacgaat attcacaatt catcgattcg ccgtataata ttcgagttga
    13321 atcagaatat gcagagtttt atcgaatcaa tcgtgtgcgc ttttggcaaa atatcaacca
    13381 agacccaaag ctacaacact atgtaaaaga gtattaccgc tatcgtctgc tttattcgat
    13441 gaaaaaaatg cagcaaatgc tattaaacgg aaagctggga gctgtttgta cgcaacttta
    13501 cgaattatat gatttattcg gtgtgcggac agaagaaggg atgctgatcg atttttcgat
    13561 cacgaatgaa gaaatcgcca aattttgtgg catctcaact gctagcagtg taaacagaat
    13621 tttaaaacaa ctgaaaaaag atggtattat caccgttaaa gaccatcata tcgttatttt
    13681 gaatatggac ttgttagaag acaatattat cttttgatga acaaagaggt tgtgaaatca
    13741 gcgttttgac ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    13801 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt taccaagagg ttgtgagcct
    13861 gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa
    13921 tattgtgact tgtgaccgag gagttggtag gagaacaccg tttaccaaga ggttgtgaaa
    13981 aagctgttct gcatcaagta ataagaagag ccaagtaaaa acagtctctt atgtttttaa
    14041 cttggctgga cttatcaccg tagatgtaag cttttgaacg ccgtttattt gcaacaaaag
    14101 gggctgtgac acgatttttg tcacagccct ttttttgcga acattcattt agcaattttt
    14161 agaaacttct gccgccaccg ccaaaagtcc cgccaccgct agaatgggta gtacttccgc
    14221 caccgccgct tccaggaggt gggcttttgg ggattcttct tgtcgtgaca aaagaatttg
    14281 tcagacgatc ggttttttca gttaatttga tactggattt ttctcggaat ggatatttgt
    14341 agatgcctga tttcaattga taacgcgaaa cagtcacaaa ataaaagaca agactaagga
    14401 taagagccaa aacaacagca atgatgattt ctactgtcgt caaaacctta tagcgtgtaa
    14461 ttttgccagt ttcttcgtct acacgataat gtcctcctgg aactccgtct tccacatatg
    14521 ttgctgattt agacaggtaa gcttttgcag cattgtaata ggcagcattt gtcatctgat
    14581 cataaacatc atctaagatc gaatcgattc tgctatcagt caaataatcg atcatattac
    14641 cagaagtcga aatgtagatt tcacgctgtc ccatatctaa tagcaatact gagccatttt
    14701 gatcatttcc gactgcgtct cttaagtaat tatcagcaaa gtctcttggt tcttctgaat
    14761 tagatgtagt cgttacgatc aagactcgtc ctttgatttt ttcattcagc ttttctgcct
    14821 gttgttccaa agattggatt tgatctgttg tgaataaccc agcctcatca tttaccgtag
    14881 ctgtatctgc atgagtagcc accggccagc caacacttac tagcaaaaga aagaataaaa
    14941 aaaatttttt catcatatca agtaacctcc gatcagaaag aggatcgcta gcccagcgaa
    15001 aatcccaaga gcggtcagtc ccaatttttt ctggctgatc ggcaggacac cgctgacctt
    15061 tcctgtttgt ccattcattg cgtagtagta gactttttta cttgagtcat tgcttcgata
    15121 agtaacaagc cataccggca gtaatacgta attatttact tcgtttgtga ttgaagcaga
    15181 agttcgcaca ccggtcaatg ttgtatatcc atttgcggta tcttttaaca gcttctctga
    15241 atagtctttt aattcagatt gaatctgtga tttgattgcc tcatattcga tatctctttt
    15301 ttctgcttgg aacccggcta aatactggct tttaaaatca attgctttat ctaacgggaa
    15361 aggttgcacg ccttcgacca tcttttgttg cgtatttttt gataaggcat tttttactag
    15421 ttctttaaaa gaaagcttgc ctgctcttga aacagcaaat tgcttcgttt ctgtgtattc
    15481 gatgtcacct atccgccata tgcggataac tgtaccattc gcctgcatgg caccatctac
    15541 ttcagcatct gtagcccaat aagggaaata cacacctgtc aacttatcga tttgatcctt
    15601 attgaaaaaa gctttgggaa caaaccattt tttcttggtc caagcgagaa atctgttgat
    15661 tgcttcttct ttctcgacag caaaaggaag tactttattt ggcaagaatt ctccgcttaa
    15721 acgtcccgat agtacgacgg gattatggca gtagtaacaa taagtcgcag cagttgtcgc
    15781 atcagtcacg atctctgcac cacagttggg acaaagaaaa agatctatcg ctgcggtatt
    15841 atctgcagat tcgtccgctg tatctgaaaa accgcctgcc tcgtcaaccg ctttgcgctc
    15901 ggcttcattc atatcggcaa gctgttcttg ggctgtaaaa gtaaaatctt cggtaggtga
    15961 aggttcttct ttgctttctt cgccagccat acgcgcttct ttttgctttt gctcatattg
    16021 gctgacttct tcttctgtat agatattcag gcagtattcg caatgaaatt tttggtcttt
    16081 gggatcaaaa gtcaacggac cgccgcaatt aggacattta tgtgtaagaa catccatcag
    16141 ctgcacacct ttctaaataa attaattaac ggggattcct ttaaacggtt taccacagtt
    16201 tggacagaat ttagggatgc cgttggaaag atccacgatc tcattgcatt cactgcatcg
    16261 catttgtagc ttaggctgaa cgttttctac aggtttggca gctccgcagt tggaacagaa
    16321 ttttccggaa ttttctgtcc cgcaaactgg acatgtccaa gtatccgcat ttccttgtgc
    16381 agtttgttga ttttgttttt ctgcttgttg tttgatttgt tcttggttat tttgagctgc
    16441 ttgagaaaga taagaaccat ttgcattcat tcccatacct acacccataa agccggtcat
    16501 tgctcctgct tcattttttc cggctgcttc cataccacgt gcaatggaac cttgcacgta
    16561 tccttcacgt acgctaggat cacctagcat cgccccttcg ttacgcatat tgatcagttt
    16621 cacagaatca tcggtgtaag aaatgctggc tacagccaca gaaacaatct ccatcccacg
    16681 taattcacgc caagaatcat ccaatgcagt atccatgtat ttgcttaatt ccaaactctt
    16741 agatggtaca taagaaatcc gttgaccatc tgcagacatc tgattgattg ctgattgtag
    16801 cgctgttagg aattcagcta aatactgctc attgatatca ttgatttcca cttgtgtttt
    16861 gtttttaggg attgcgtttg tataaaataa aatcgggtca acgacatgaa tagaataagt
    16921 gccatgtgcc cgtaagaata attctgcgtt atagaaattg tcaaagtagt tcaacggcgc
    16981 gcttgtaccg aatttgattc cctttatttc ttggagattg atataaaaaa cttgttgttt
    17041 ctgcggtgtg acgccgccaa atttgaatcg gtcaaaagtt tcagcaattg cttctttcag
    17101 tgttccattg aacatggaag gagctgcgtc atttttaatg gtgtaataac cttcctcagc
    17161 ggtataatcg atgatttttc caccatcgac caaaagcatc atcatgtttg gatagacatg
    17221 gacgactgtt ccgtctgtta aaacatcttc tgttccttta cgattggagc cacgtttgtc
    17281 atctttgcgg actttgacac cttttgtcat gaccgttgta tcactcatat tgtctggttc
    17341 gatgacttct agccattgat ctgccagacc gccgccaacc atacttgttg ctgctttgat
    17401 aagacccata aaaaattcct cctctgattc agctagtgcc aaacaattat tgtttattta
    17461 ttataccata ggaagatgtg aaacttctcg gactagagac ggaattgaaa tggaaaattg
    17521 cgtattgttt aaatacctga aaaagccgag aaggatagta gtttttttac ctaatatcac
    17581 gtataataag agatagtgaa ttttttaaaa aagggggatc cttatggcat atcaagcact
    17641 ttatcgagtc tggcgttcgc agcgtttcga agacatcgtt ggacaaaaag ctgtgacaca
    17701 gacactaaaa aatgctattg tttcccacaa aacctcacat gcctacttgt ttacaggacc
    17761 cagaggaacc ggaaagacga gtgcagcaaa gattttcgca aaagcaatca actgtccgaa
    17821 cagtaaagac ggtgagccat gcaacgaatg cgaaatgtgt cgatcaatta ctgctggtac
    17881 gcaagaagat gtgatcgaaa tcgatgctgc cagcaataac ggagtcgaag aaatccgatt
    17941 tatccgtgat cgggcaaatt atgcgcctac aaaggcagcg tacaaagtgt acattattga
    18001 tgaggtacat atgctttcaa caggagcttt caatgccttg ttgaaaacat tggaagagcc
    18061 aaaagaaagt gtcatattca tcttagcaac gaccgaacct cacaaaatac cagctacgat
    18121 catttcacgg actcagcgat ttgattttaa acggatcaat acggcagata ttgtcgaaca
    18181 tcttgaattt atcttgaaag aatcgactat cagctatgaa ccagctgcat tgaatgtgat
    18241 tgcccgttct gcagaaggcg ggatgcggga tgcattgagt attcttgatc aggcaatctc
    18301 gtttagtgaa ggaacaatta ctttagatga tgccatgcaa gtgacaggta gcttaactga
    18361 cgaaatgatg gacagttact tgagtgcttg cgtgaaaaaa gaagtaccag aagcattgga
    18421 aacattgaac caaattctcg cagccggcaa agaatcacgt cgctttctag aggatatgtt
    18481 acaatactgc cgtgacttgc tgatgtatca gcaagcacct aacctagttg aggaacaatc
    18541 gagtacgatc agaagtacat ttacagaatt ggcacaaact gtttcagcgg atcagctgtt
    18601 ttcaatgata aagattttga gcgatactca aaatgaaatt cgttttacca atagtgccgc
    18661 tatttatttg gaagtcgcta cagtcaaact tgcacagcta ccgtctgctt ctgttccttc
    18721 agcagatgta gcatttgcag atgatcagat agtaaaacag ctacaacaag agattaagga
    18781 actaagaaac gaaatcaagg aactacgcag taatacagga attgagaata caaaagtaca
    18841 gccggtacag caaacaaatc ataaaaaaca gacaagtacc tatcgtgtac cgacggaacg
    18901 agtatataaa gttctgcggg aagcgacaag acagcatctg aaccaagtaa aagaagtatg
    18961 ggaagacttg ctgatgagtc tatctgtcac tcagcgagca atgcttaaag caagcgaacc
    19021 agttgcagct ggacctacag gactagtgat tgcgtttgat tatgagattg tttgtcaaag
    19081 agcagcaaat gacgaagaat tgcagctttc ggtacacaac aacttaagcc gaatgatcaa
    19141 agattatgcg cctgatcctg tttatattac aagagaaagc tggccagaac ttcgaagaaa
    19201 ttatttgacg caggcacaag aagagggaac agaaaactct tttgcttctg aagactcaga
    19261 aggagaaatc gagttgcttc ctcaagaaca agaagtacca gaagaaacac ttgtagtaaa
    19321 agcagaggaa ctgtttggct ccgaagctgt tacagtggta gatgattaat aaaaaggagt
    19381 agataattat gatgcgtgga atgggcaata tgcaaggcat gatgaaacaa gtacaaaaaa
    19441 tgcaaaaaga aatggcagaa gcacaagagc tgctaaataa aacagagttt acaggtaaag
    19501 ccaccaatga attggtcaaa gcggttttta caggcgatcg tcgcatgaaa gatatccaaa
    19561 tcaatgaaga agtcgttgat cctgatgata tcgagatgct tcaggatctt gtcgtaatgg
    19621 cagtcaatga tgcgatcgca caaattgatg tggaaactga gaaaaaatta gggaaatacg
    19681 caaaaggttt gccaggattt tagttttaaa ctgaaggagt aatgaatatg caatatccag
    19741 aaccaattgc gaaactgatc gacagctaca tgaaacttcc aggaatcggt cagaaaacag
    19801 cgacaagact cgccttttat acgatcgata tgaaagatga agtagtaaat gaatttgcta
    19861 aatctttgtt aagtgtaaaa cgcgatttgc atttttgtag tatctgcgga aacatcacag
    19921 aagaagatcc ctgcgaaata tgtagaaatc cttcacgtga tcgaagcgtt atcctagtgg
    19981 tcgaagagcc gaaagatgta atggcaatgg aaaaaatgcg agaatatcac ggactttatc
    20041 atgttctcca tggtgtgtta tcaccgatgg aagggacagg tccagaagac atcaatattt
    20101 cttctctgtt gcagcggctg catgatgcag atgtaaaaga agtgattatc gcaacaaatg
    20161 caacaacaga gggagaagca acagctatgt atctctcacg tctgatcaaa cccgcaggta
    20221 tcaaagtgac tcgtctagca catggtctgt ctgtaggctc tgatatcgag tatgcagatg
    20281 aagttacttt attgaaagct gtcgaaggaa gacgtgagtt ataaatttct aattaagtgt
    20341 tttttcagat acaatataaa agcagcacaa gagatggggg caacaatgtg aacggactat
    20401 ttattacaat cgaggggcct gatggtgcag gaaaaaccag catcttgaat gaactttacc
    20461 ctcttttgaa agaagtagca aaaacagaaa tcattcaaac aagagaacca ggaggcattc
    20521 cgattgctga agagatccga gcagtgatcc ttgatccaaa aaatgatcgg atggatgaac
    20581 gtacggaagc actgctttat gcagcagcaa gaagacaaca tctagtcgaa aaggttctgc
    20641 ctgctctggc aaaagggaaa atcgttttat gcgatagatt tgtcgacagt tctcttgctt
    20701 atcaaggtgc aggccgacgc atcggcgtta ccgagattgc tcggttgaat gagtttgcta
    20761 cagaaggtac tacgccggat tttacccttt atcttgacgt agattcagac accggattaa
    20821 gaagaatcaa gaaaaacaga caaaatcaaa tcgaccgttt agattcggaa ggtttggaat
    20881 tccaccagcg tgtacgtcat gcttacttaa agctggcaga agagaatccg gaaagaatcc
    20941 acaaagttga tgcaagaaaa agctttgaag aagtgctgca aacaagttat catacaatta
    21001 ttgaacagta tccgcaattt tttgaaaact aggaaggtga acgtttatga aaattatttt
    21061 agcgattatc caagacaaag acagcaaccg tttatcaaat gaattgattg atgcaaatat
    21121 ccgtgccaca aaactatcat cgacaggcgg cttccttaag gctggaaaca gtacgtttat
    21181 cgtgggtatt gaagatgacc gtgtagaaga agcattggaa atcatcaaaa aaacgtgtga
    21241 atcaagaaag caatttgtat ctacacctgt tacactggat atttcgatgg atgggggcgt
    21301 cccttatcca gtagaagtag aagtaggcgg tgccacagtc ttcgtcttac ctgttgaagg
    21361 attccatcaa ttttaggagg agctgagcta tgaatcaaga aataaacttg gctcaaatgc
    21421 aaccgatcgt gtatcagcag cttcaacgga gttttgagca tgggcgtatc gctcatgctt
    21481 atctttttga aggggagaaa ggaacaggaa aacatgaagt aggtatatgg cttgcacagc
    21541 acctgttctg tacccaaatg aaagacaatc tgccttgtgg aaaatgcaac aattgtcagc
    21601 ggatcgaaaa gaaagaacat ccagatgttt tggttattga accggaagga caaaccatca
    21661 aagtcgatca gatcagacgt ttgcagacgg aattcagtcg cagcggttac gaatcaagaa
    21721 aaaaagtttt tttgatcaaa gaagctgaaa aaatgaatag cagtgcggca aacagtttgt
    21781 tgaaattctt ggaagagccc cctggagatt ttttagcggt gttagaaaca gatgcagtag
    21841 gaagaattct ccctactatt caatctcgtt gtcaagttct gcattttcaa gaactttcga
    21901 aagaagcact gatccatcga ttgcaagaag agcagattcc attggaaaaa gcaaaacttt
    21961 tggctttctt aacaaacagt ttaggaaaag cagttgaaat ctctcaagat gaatggttta
    22021 atgatgctaa ggacttgatt tatcaatggt tcgtttactt gcaaaagcaa gatactcaag
    22081 cctttatcta tgtccaaaaa aaattggtaa aatcatttaa agaaaaaaca caacagttta
    22141 caggattatc tgtcttgatg ttttattatc aggaagcaca gcgtcaagca gtagcaaacg
    22201 gcgaatatgg caaagtgaaa cagataaacc agataattga acggatattg atcgcagagc
    22261 agaaactacg cagcaatgtc agttttcagg ctgttgcgga acagttcgtt ttgcatacga
    22321 ttttttagct tgccgcaaag gatgatgatc aaggaggatg gaaatggtag aagtagtagg
    22381 tgtccgatat agagatgcag gtcatatcta ttattttgca ccaggagact ctgaatatgt
    22441 ttatagtgac aaggtattag tcgaatctca gcaatcctta catattgcta ctgttgtcct
    22501 tcctaaaaaa gagattgatc ccaatgacct tcctgaagct atccgtccga tcttgcataa
    22561 agcgtcacaa gctgaattag aaaaagacaa aaaaaacaaa aaagatgctt atgatgcatt
    22621 gaaaatcgca aaagaaaaag tgcagttgcg tgagttggaa atgaaaatgg tcaaagccga
    22681 atacacattt gatcgaaata aattgatttt ttctttcaca gcggacgggc gaattgactt
    22741 tagagaattg gtaaaagatc tggccgctgt atttcgtact cgtattgagc ttcgacaaat
    22801 tggtgtccgc gatgaagcaa agataatagg cggaattggt ccatgtgggc gtccattatg
    22861 ctgttcttct tttttaggcg atttcgtgcc tgtatcaatc aaaatggcaa aagatcaaag
    22921 tttatctttg aatccaacaa aaatttccgg tttatgcgga cgcttgatgt gctgtctgca
    22981 atatgagagc gaagaatacg agacagcgaa aaaagaactc cctgactttg gaaaagtaat
    23041 tgctacacct gatggtaaag ggaaagtcgt agggttgaat ctgcttagcc gtatcgttag
    23101 tattcggctg actggacggg aaaatcctgt cgaatatgaa tgggatgaat tgaaagaatt
    23161 tatccaagta ggtgagatga atggataaga gatcattata cgacggattg aatcaattag
    23221 aatcagagtt gagaaataca ttaacagaac ttgttgaaat gaagcatgct ttgcatgaga
    23281 tcgtagaaaa aaatacaact ttggagatgg aaaatcaacg tctgcgggaa cacctgcggg
    23341 aattgaacca agttgcacaa gctccagctg aaaatgtgaa gcaggaactt tctaagtctc
    23401 gtatgaatct tgaaaaaatt tatgaagaag gattccatgt ctgctacgat ttatatggat
    23461 caagaaggga caatgacgag ccttgtgcgt tttgtctaga agtgatctat cgcgaaagcg
    23521 gtcgatgacc aataacaaga ggagactgga gcggagaaat actgctccag tctttttatt
    23581 aaggagattg tgacagatgt ataatcaaaa aagtttcaag ggaaatccgg tatatgggga
    23641 tttatatctt gtaccgacgc caattggaaa tttagaagat atgagctaca gaagtgttcg
    23701 tatgctgcaa gaatccgatc ttatcgcaag tgaggatacg cgtaatacac aaaagttatt
    23761 gaatcatttt gaaatccata caccccaaaa aagtttacat gaacataatt ataaagaacg
    23821 tgtccctcaa ttgattgctt tgttaaaaga aggaaaaacg attgctcaag tcagtgacgc
    23881 aggaatgcct tccatcagcg atcctggaca tgagctagtt ttagcctgca taaaagaagg
    23941 gatttctgtg atctctatcc caggacctac agctgggatg acggctttga ttgcttcagg
    24001 gctagtgcct caaccatttt tattttatgg cttccttcca cgtaagaaaa aagaacagaa
    24061 agaaacattg aaaaagctaa aagaacagac agttacgctg attttttatg agtcacctta
    24121 tcggatcact gctacattga cgaatttctt agaaattttt ggaaatcgcc aagtcgtttt
    24181 gtgtcgggaa ttgacaaagg tccatgaaga ataccttaga ggaagtacgg aagagctact
    24241 cgcttatatc gaagaacatc cagtaaaggg tgaatgttgt ctgcttgtag aagggaattt
    24301 gttcgaaatg ccagtgacgg aagttgatga agagttggga acgttaaagg aacaagtaga
    24361 agcaaagatc gcttcaggcg aaaaaccaaa tgctgctatc aaagcagtag cgctgaaaaa
    24421 tgggctgaaa aaacaagaag tctaccgtca gtaccatgaa ttagactgag atagtataag
    24481 ttgtctataa tgaaagacta atgaggaaat cagctactaa cttttcaaat ctcttgaacg
    24541 cgaacgaatg ttcttgtata atgaataaga ggtgaggatg atggagctgc aatttccttt
    24601 acataatgat acatcaagaa aaatcattca tattgatatg gatgcttttt tcgcttcggt
    24661 tgaagaacga gatcatcctg agtttcgcgg acatccatta gtaattgcca gacaccccag
    24721 tgacactggt gggcgtggag tagtgactac agcaaattat gaagcaagaa aattcggaat
    24781 tcattcagca atgagtgcac aaaaagcgta tgagctttgt ccgcaagcca tctttaagcc
    24841 tggcgaccat cataaataca gcgatatttc caaacaagta cgggaagtat tctatcgtta
    24901 tacagatatt gtggaaccag ttagtatcga tgaagcgtat cttgatgtga ccgaaaataa
    24961 aatcaattgc aagtcagcca tcaaaatcgc ccgaatgatc caagcagata tttgggagag
    25021 tgtccagcta acatgttctg cgggagtaag ttacaataag tttttggcca aattagcatc
    25081 agactatcaa aaacctagag gaatcacggt agttgcccct caagacgctg tgccattttt
    25141 aaaagctttg ccaattgaaa aatttcatgg ggtagggaag aaaactgtac ctagaatgca
    25201 tgagctaggg atttatacag gaaaagacct atatgaatgc acagaaatga tgttgattcg
    25261 aaattttgga aaaatgggct attctcttta tcgtaaagtc agaggaatcc atgattctcc
    25321 agtaaacgtt acaagagagc gaaaatctgt gggaaaagag catacttatg ggcagccgtt
    25381 gcaaacggaa gaagcagtgc tgactcagtt gcgccaacta gctgaaaaag tagaagaagc
    25441 actgagaaga gtccaaaaac atgggaaaac agtagttttg aaggtcaggt acacggacta
    25501 ttctactgtg accaaaagag tgacattgcc agaatatatc tataaaaaag aagcactctt
    25561 ttatcaagca agccttatat gggaggaaat cttgggagta gaaaaaggaa tccgattgtt
    25621 agggattact ttgacgaatc tggacccgat gacttatgaa aacatcgtcc ttccattatg
    25681 ggaaaatcaa gaaatataaa aacagctttg agagttgaaa ccggaaatcc gttttctctc
    25741 ccaaagctgt ttttatatca agtcttgctt tttgatttgt tcataggttt ctgttgcatc
    25801 cgtacatttt tcccaaatca caacttgatt ggattctaaa gatttttgga cgtcaatgat
    25861 ccgctggttg ctacttccgc gaaattgtag ttttaggtca cgtttgctta attcaaagcg
    25921 tccgtcaaca aggatatcga tttgcgaaag gagttctaat ttatcttctg aatcttgtag
    25981 caattcatca aaggtatagc ctgtccatga ccagatatct tttgtcgtac cgaactcttt
    26041 tcggatacgt cgtacgactt gcagacaaac atctgtattc aaaaaaggtt cgccaccaag
    26101 taaagttaat ccttgcacgt aatcgttgcg caagtcagcg atgatttgtt cttctaattc
    26161 aggtgtgaat ggtttgccat atcggaaatt ttgtgcagct ttgttgaagc agccttcgca
    26221 ggcaaataag cagccactaa cgtaaagact gttgcggaca ccttcaccat cgacaaaatt
    26281 aaacggtttg tagtcagcaa tatagttttg gctgtagtct tttgctaacc attcttttgg
    26341 ttttggattt ctcataaaaa aacttctttc atttaaggtt caaagatata gctattgaag
    26401 ataaaatcta ctcgaccaca taaataagaa caaactgacc taaatccatt gttcaagaaa
    26461 gcttgttctt attttgatag agggcgttca aagatacaaa gccatcgaaa gagggtggtg
    26521 aaaaaagact tgtcatagcc tcttattctg aataaacggt gttcaaaagc ttgcatctgc
    26581 agtaataagc ccaactaaac gaaaaatatg aggaactatt tttgtttagc tattcttatt
    26641 acttgatgca ggacagcttt ttcacaacct catttcatgt gtttcacacg tgaggaaatt
    26701 tctttatggc ggccatgtac catcggacga gcttgcggat ttcctaaata gccacaagta
    26761 cgtttgacta catcacatgt ttttggatca tgatttccac attctggaca ttcgaaccct
    26821 cgttctgtcg gtttgaaatc accttcgaaa ccacattgat agcaatgatc gattggcgta
    26881 ttcgtaccaa gatacccgat tttgtcatat gcaaaatccc atacagcttc taaagctttc
    26941 ggattttgtt ggagcatagg gtattcacag tagtggatga atccacctga acaatatttt
    27001 ggataatcct tctcaaattc caatttttca aatggcgttg ggtttttacg cacatcatag
    27061 tggaaactgt ttgtataata ttctttatct gtgatatttt cgatgatgcc gaatttttct
    27121 gtatccaaac ggcagaaacg gtcagttaga ctttcacttg gagttgaata gacactgaaa
    27181 tgataaccat attcatcccc ccatgcatct gcattagctt ttaattcttt gacgatatca
    27241 agtgtgaaag cttttgcttc tgggtttgtt tcccattcgc caccgaagaa agctgaagca
    27301 acttcataca atccaatgta gcctaacgat acagtagctc gacggttttt gaataattca
    27361 ttgacagaat cggtacgtga aagacgtttc ccgaaagcac catacatata taaaattggc
    27421 gcatttgctg gaatggcttc tttacaacgt tctacacgat agagaagcgc atctttcatg
    27481 atttctaagc ggtcagacag taactgccag aatttattct gatctccttt tgattcgatc
    27541 gcgattcttg gcaaattcaa tgtaacaaca cctaaattca ttcgtccgac atttacttct
    27601 tgaccatttt cgtctttcca cccttgcaag aatgaacgac agcccatagg tactttgaaa
    27661 cttccagtta attcaacgat tttatcgtaa ttcagaatat caggatacat tcttttcgtt
    27721 gcacattcca atgctagttg tttgatgtcg taattaggat cttcagggtt taagttcaca
    27781 ccttttttca aagtgaagat cagtttaggg aagatagctg tccgcttttc actccctaat
    27841 ccattgattc gaatctgtaa aattgctttt tgaatttctc tttcaaacca gttttctcct
    27901 agtccaaatc ctaatgaagt gaaaggagtt tgtccatttg aagtaaataa ggtattgatt
    27961 tcatattcca aactttgcat agcatcgaag atatcttttt tggtttttgc ttttgcatat
    28021 tctttttggc gttctggcga atcgatccat tcttccgcgt cttttagatg ttttttatag
    28081 ttcaattctg caaaaggagc aagcaattca tctgttcggt cggcagaaca gccaccatat
    28141 tgacttgacg ctacattggc aataatctgt gagatttgtg ctgtggcggt ctggatagat
    28201 tttggcgatt cgacttcggc attgccgatt ttgaacccat tgttcagcat gcctttgaaa
    28261 tcaatcaagc agcaattcgt catcggtgta taaggatgat aatccaaatc atggtaatgg
    28321 atatcgcctt tttgatgcgc atttgctata tgtggtggca acattttcag tccgattgat
    28381 tttccaacga tcccggctgt cagatctcgc tgtgtattga aaacattact gtctttattt
    28441 gcgttttcgt tcacgacagt ttggtccttg ttgattagct tttcaatcga aaagttgata
    28501 tctgttgctt tactgcgagc aaaatctcgt ttggtacgat aattgatgta ctcttctgct
    28561 aattcatatt cgtttttttc tagtaatata tgttccacga tgttttgaat ctcatagatt
    28621 ttgacatttt cagcaaaccg gcttttgatt tcttggacaa ccaagtctgc aatcatttga
    28681 attttttcat aagtaagtgg tgataatgag ccgtgaattt tcttttctgc tttgatcagt
    28741 gcttcattga tcttttgttc atcgaaatca acatggcggc catcgcgttt gatgactttc
    28801 atctgggatg tctctggctg ctgagtaacg acagtttttt cttcataatt tactttcatt
    28861 atcttcaact cctgctaaat tgttcttgtc tatcataaaa caaaatcggg gaaggagcaa
    28921 ctaaatatcg gctatatata gtattcaatg tgctgtataa ttggatttct atactatatg
    28981 tagtgtatat tttattgtga aaaaaggtga aaacttgtag ctggtggatt tgttttttgc
    29041 atatttaatt ttgctgaata aaataattga ataaaatttt ttattcagtc caagtaaaag
    29101 tgacaaaaac ataagagcaa attattcgta gatatgctac tatgcaaata aatagaaatg
    29161 aaaaatgaaa gaggaggaga agaattggaa aagatgatcg aagtgaaaca tctaagtaaa
    29221 agctacggca gcaaactttc tcccgtcaaa gccttagata atatctcatt cacagtagaa
    29281 gaaggcgagt ttgttgggat tatgggccca agtggtgcag gaaagacaac gctgatgaat
    29341 atcttgtcca cgatcaattt gcctacgatg ggcagtgtct cgatccaagg aaaagaaatc
    29401 acaaaaatga aaaacagtga gctcagtgac tttcgtcgaa aaaaactagg attcattttt
    29461 caagagttca atttgatcga taccctaaat gcaaaagaca atatcctatt gcccctagca
    29521 gttgaacgga tgactaaaga ggaaatggaa aagagagtaa gacatgtagc tcaattgttg
    29581 aatatagaag aactgctcaa acggtatccc gatgaacttt ctgtcggaca gagacaacga
    29641 attgcagctg ctagagcact tgttgttcag ccacaagtga tttttgcaga tgaaccaact
    29701 ggctctttgg actcgaagtc ggctacagaa ttgttgaact acttgaagac catgaatcaa
    29761 aaagaaaaag caacgatact gttagtcacc catgatccat atacagcaag ctactgcgat
    29821 agaattttat ttattaaaga tggtgtgatt ttttctgaag ttgtcagaag agggacaaga
    29881 agagagttct ttgagaaagt aatagacatg caggcgacga ttggcggagg aggaaaaatg
    29941 aatgctgtat aaactagccg ttaaaagttt ttttcggcag tcacgaggct atcttgttta
    30001 ttttttcagt ttgactttat ccgtaatgat ctattattcc ttcagcgcaa tgacctacga
    30061 tcaatcttta gtcagaagag caagtcaaga tacttcgata gatggtggat tgagttttgg
    30121 tggatttatc attactgttg ttttgctttt tttcgtattc agtgccaacc gatttttctt
    30181 gaatagaagg caaaaggaaa taggcatcta tcagcttttt ggtatgaata aactgcagat
    30241 atcaggtata tatgtgttag aaatcatgat aattggattg ttcgcctgta tttctgggat
    30301 attactcggt attatttttt ccaaactctt ttcaatgatt ttggttcgaa tgatggatat
    30361 ggatcttact agtccatttt ttatctccat tccatcagta gtagatactt tatttgcttt
    30421 tttcatcatc cttttagcag tatctgtata ttcaatatgg aaaatatggc gctatccaat
    30481 gattcgaagt tttggagaaa aagaacaagc agatagttca acaatgcgct tcaggacaag
    30541 gcaccgatta ctcgcagtca tcgggttact acttttaacg agcggatatt ttggagctag
    30601 tcattttagg gaaatcactt catggatgat cagttatcat tatcttgata cattagtttt
    30661 cttttttcct tttttaattt ttttcgtttg tgtggtcggt acttatttgt ttttctgtta
    30721 tacatttcgg ttatttttga tcatattgag taaatggaag atttattact atggtatcaa
    30781 ttttttgata atgggtaaca cgcaagttca tttgctcaaa agttggcgta caaattctct
    30841 gattacagtc attctcggaa tttctttaat gacaattggc ggcacggcta gtatctctac
    30901 tattttatcc taccaggaga aagtttcctc cccgatggac tatcagtttg atgttgagac
    30961 agagaaaaaa atcaagccga ttctagcaga ggaagggcag aagataacta aagaaatcac
    31021 cttgcattat aaagtcattg ggagtatcta tagtcaaaat ctcgaagaac ctatgtttca
    31081 aactgctaat ctgatcacgg aaacacaata tcaagccttc agaaaagtta atccagattt
    31141 gccgaacatt tctttgaaat cacctggtca tactgttttg cttgatcaaa tgcaaactct
    31201 ttttcggaat atttcattat atggatctag tattggactg ccagataatc aagaattgaa
    31261 gattcaacaa atgcttccaa gtgtattagg tgacgaaaca atggtatatc taagtcctgt
    31321 actgattgta gatgataccg tttttgaaaa agcaaaaggt gtggattatc aagtagtcaa
    31381 tgtagatgta tcaggtggaa ataatgaaaa aatagatgaa cgactgagca atgacctgtc
    31441 gataaagtgg ctcaatgctg tttattattc ttatgatatc gtaaatggaa gtctacaagg
    31501 agaaattagc acaaagaaac ttccggatga ccaaatgaag gaagcacaat ttagccaaga
    31561 aagctctcgt ttgaatttct cttccagcta ttcgaaattg cgtgcagtta atcggcgaat
    31621 cggtatcaat atttttgtca cactatttgt cgggatgatc gtgattattg ctactggtag
    31681 tattttgaca gtccggcaat tagcagaagc agaggaagaa aaagaaaatt atcacctatt
    31741 gactaagctg gggattccac aaagaaggat cagacggatg atcttcgagc aaaacgcact
    31801 taccttcttt ccacccatga tattagggat cactcacgca gtatttgcaa tcaatgtctt
    31861 cactcagtat ataaaaactg ccgattactg gttggcttat cttgtgagtg gcttattgat
    31921 ccttatctat cttttgcttt atgtcattac gagtcagtta tattgtcgga ttatcgagga
    31981 atgatatacg gaataagaca agataataaa tagaactaaa ttttgaatac catttatttg
    32041 gaataagagg ttgtgacacg atttttgtca caacctcttt attttttata ttttttaaaa
    32101 gtacgagaaa ttataaagaa aaaagcagtg atttgatgtg ctatttttat aaaagctatg
    32161 tgacaaaact gttagtaaaa aaacggtttc tgtaagctag acacatgttt ctttttgtca
    32221 aaattagtta cactttgtgt gaggttatat gaggagggaa tccaatgaac aaatggcgtt
    32281 ctaatccaaa attgaattat tggggaaaat tcatcattcg aacaattttt tatagtgcag
    32341 tactgctgtt cctgatttat ctgtatcact ataaaaatat tcaaggagga acttttatct
    32401 ataacgaatt ttaggaggaa gagtatggaa atcaaaacga ttattgaagc aatcgatgat
    32461 tggggaataa atgaacctga tcgagttgct tatcaggcgg atgagacgca tacttttggt
    32521 gaattgaaac aagcatcaga cgcacttgct tattatttag aaaaaaaggt agaaggcgat
    32581 ggaccaattg tcgtttttgg caatttggag tttgaaatga ttgtctcatt tctaggggtt
    32641 gtcaaatctg gccatgccta tttgccgatt gaagagcata cacccaagga gcgtatccta
    32701 tcgatctttc gggtggctaa accatctatg gtcatcagta tcggggactg gatagaagat
    32761 attccttcgg ttcctgtcat aacaaaaaaa gaatttcaag aaatcaagaa gataccagta
    32821 ggattttatc ccagaaatag cgtaaaaggt acagaaaatt tttatattat ttttacttct
    32881 ggtactactg gagaaccaaa aggcgtacaa atcagtcatg ataatctagt cagctttgtt
    32941 caatggacga tggaagactt tgggatcaag cctggtatgc atttcttagc ccaagcgcca
    33001 ttttcttttg atttatcggt attttctatt tatcctgcat tagtttctgg cggaatgctg
    33061 aagccgttaa ataaagcagt tgttcaagac tttcgtcaat tattcgcgac attgccagcc
    33121 ttgaagttga atgtgtgggt ttctactcca tcatttatgg atatttgctt gatggaacct
    33181 acattcaatg gagaaaatgt gccggaattg gacatgtttt tattttgcgg agaagaattg
    33241 acgaaaaaaa ctgcagaaag tctacttgat cgattcccaa atgcacggat atataatact
    33301 tacggtccaa cagaagcaac tgtagctatt tcaagtattc agattgacca gcatgtacta
    33361 gatagttatg accgactacc gattggctac gtaaaaaaag acacgaaagt ttctattgtc
    33421 caagaaggta aacctgtttc aaaaggagaa acgggtgaaa tcattattgc aggaccaagc
    33481 gtatcaaaag gttacctgaa caatcccgaa aagacagctg ctgcgttctt ttgtcaaaat
    33541 gacttagcaa gttaccatac aggggatgca ggtagactag atgaatatgg gcttttattt
    33601 tatgaaggac gtatggatca gcaggtcaaa ttacatggat accggattga actcggggat
    33661 atcgaacatt atctgctcca agacaaccga atcaaacaag ctgtcgtcgt accaaaatac
    33721 caaggaacaa aagtacagca gctggttgct tttgtcgtat tagaaaacaa aacgaaacag
    33781 ccggattttc agttgactaa atctatcaaa gaacaattac tagaagttgt aatggattat
    33841 atgattcctc agaagttcca ttatgtcgat ctgctgcctc agacagttaa tggaaaaatc
    33901 gatcgaaaaa aattaacagc tgaggtgaat cctgaatgat gttacctcat atgatccctt
    33961 atgcagagcc gttttatttt tttcttttag ccattgcgct tgtaccgatc gttctatctt
    34021 tattgatatg ggaaaaaaga ttaccagttt atcaatcagc agtgacactt ttctttttat
    34081 ttgtcagctt tggaggtaat tattggcaac agggagcggc gctgattggg tatgttttgt
    34141 ggcagacgat acttgtatgg ggctattttg tttatcggca aaggaaaaat cagacaggat
    34201 ggttttttgc cgccgttatt ttagcgataa ttccactgtt tttaacgaaa gtgactcctt
    34261 tttttgaaca tggaaaagca tcgttacttg gttttttagg aatctcttat ttaaccttta
    34321 aatctgtaca aatgatcatg gaaataagag atggcatgat caaaacatat aagccatttc
    34381 gttatatcca gtttctgctg ttctttccaa caatctcttc tggaccaatc gatcgctata
    34441 ggagatttga aaaagatcag ctccatccgc ctgttcgtga taaatatgtt gatcttttgg
    34501 gaaaaggcgt ccaaaatatt tttgtcggtt ttttatataa attcattatc ggttattatt
    34561 ttggtcaagt actgcttcct gtagtgggac gcattgcctt acagcatggt ggtatttcat
    34621 gggcattagt aggttatatg tacgtgtaca gtatgtattt gttttttgat tttgctggct
    34681 atagcttatt tgcagtcggt acaagctaca tgatgggata tcagacacca atcaatttca
    34741 ataaaccttt tttatcatgg aatatcaaag aattctggaa tcgttggcat atgacgctgt
    34801 ccttttggtt ccgtgattat gtatatatgc gattgatgtt cttcatgatg aagaaaaaat
    34861 tttttaaaag caagatcgta atgtcaaata tcggttattt tgctttgttt cttctaatgg
    34921 gtatatggca tggattgacg tggtactaca tcgtatatgg actgtatcat gcttgtctga
    34981 tctgtctgac agatgcatgg atccgattta agaagaagca taaagagcag ttgccttcta
    35041 acaaatttac gcatggctta gccgtatttt tgacatttca agcagtatgt tttagcttcc
    35101 tgattttttc aggattttta gataaacttt tttttagcta acgaatagtg acatattaat
    35161 ttatataaca aaggagaaaa ggaaaatgga agaacaaatt ttatcaatct tagaagaaat
    35221 aacaggaaca gatgaagtaa gaaaaaatcg tgatattgat ctatttgatg aagggttgct
    35281 tgattcttta gcatctgttc aattattagt cgagctagac ggagagctgg atatccaagt
    35341 ccctgtatct gaattcgagc gagaggattg gagcacaccg aataaaatca ttcaacaagc
    35401 aactgcattg aagggttgag tatgaaaaag aaactttttg ctattttcgg cccggttgtt
    35461 ttagctgttg gcttactgtt tgtgtttttt acctcctcct atcaagtaga tatctctaat
    35521 ccagaaacga ttagacgtgc ctcttcttcc atgtcagtga acgtgttgaa aggagatgcg
    35581 ataaaaaatt atgcattatc tgaaaaacaa tatattcctt tttttggctc aagtgaatta
    35641 agcagaatca gtccatttca tccgtctgtt ctggcagaaa aataccagcg aaactatcgt
    35701 ccatttttgc tgggagcacc cgggacacaa tcgttaagcc aatatatgat gatgcgttct
    35761 gcaggagatg ctatgaaaaa caagaaagtc gtctttatta tttctcctca gtggttcgtt
    35821 aaaaatggtg taaaaactga ttactttaat acgtattatt ctgaacttca aacatacgac
    35881 tggttgtttt caatgaagaa agtaacgcct gctgatcgct atttagctag aagattatta
    35941 actttctcaa aagtaaaaga gaatgatacg ttgacggcga ttctgcaaac gatcaaaaaa
    36001 gggaagctac ctttacctga atctttgaat cagctgcgca gccaatggaa catgctgaag
    36061 agagaggatg aagtattcag caatattggg ttgaaagatc gacagcaaaa aatcgatcat
    36121 gagtcaaaac gattaccaaa acaataccaa gaaacagaat tatccatttt agcaaatcaa
    36181 attggagaac gagaaacgac gaataatcca ttcgggttga aaaacgattt ttatacgcat
    36241 cgtatccgag cccacgagcc agagctcaaa cagtcacaaa aaaattggga ttatcgtttt
    36301 tcgccagaat tttctgattt tcagctggtc ttggatcaat tagcaaaaaa ccataacgaa
    36361 gtattgttca tcatcccacc ggttaacgag aaatggagcg attataccgg actatctcaa
    36421 gaaatgttac aaggatttgc caaaaagatc aaatttcaat taaatagcca aggattcaat
    36481 cgaatagctg attttgtcaa tcaagcgggt accaactatt ttatggaaga cacgatccat
    36541 ctaggctgga aaggctggtt ggctgcagat caacagatca gaccgttttt agaagaaaat
    36601 catattactg ctagtaagta tcatcttgac gatgcttttt ttagcaaatc ttggcaacat
    36661 caaataccag ataaactaca attaaaataa agaaaacagg cgagtgttgc tcgcttgttt
    36721 tctttatttt tgaatattct gaataaaaga taaaaatacc aagtttgcct gaacaaaaat
    36781 cataagcaat ggtataatca caatatgaat atagaaatgg gggaaaacat ttggatacaa
    36841 aagaagaaca gctacttatt gatttaactt ctgcaaaagg agtacctgga aatgaagaag
    36901 aggtaagaga agtattcaga gaatatgcca aaccattcgc tgatgatatt ttttatgacg
    36961 gtttaggcag tgtgattgcc aaacatggtg ctggcggagg ccccaaggtc tttatttctg
    37021 gacatatgga cgaagttggt tttatggtaa caaaaataac cgaaaaagga ttcttggaat
    37081 tccaaactct tggaggttgg tgggggcaag tcatgcttgc tcagcaagtt gaaatcaaaa
    37141 cacgtgaagg gaaaatcgtc cacggtgtga ttggttccaa accaccacat gttttaacac
    37201 ctcaagtaag aaataaacct tatgaaataa aggatatgtt cattgatatt ggcgcatcaa
    37261 gccaagaaga agccaaagag tggggcatac gtcctggaga tatggtcact ccatatattg
    37321 aatacaaacg aatgaatggc agtaaatatt tactggcaaa agcatgggat aatcgtatcg
    37381 ggacagctgt ttctctgcga gtattagaga atctatctaa agaagcacac ccaaatgttt
    37441 tattcgcagg aagcgatgtg caagaagaag taggcttgcg aggtgctcga acaagtacac
    37501 accttgtaaa tcccgatatt gcttttgctt tagatactgg tacagcagga gacactccag
    37561 gaatgacacc aaaagaagca gattcgattt taggaaaagg cccacagatt ttgatttttg
    37621 atgcatctat gatcccgcat aaaaaattat tgaattttgt tattgatgta gcagaggaaa
    37681 tggatattcc attccaatat acagtgattt ctggcggagg aactgatgca ggacaaatgc
    37741 atttgacaag agatggtgtt ccgtctgtag ccattaccgt tcctgttcgt tatctgcatt
    37801 cccatacatc agtgattcat gaagatgatt atttgaatac agtgaaacta gtcacagaat
    37861 tagtgaaacg gcttgatagt gaaacagttg aaaaaattcg gagttatgaa taatgaataa
    37921 tagaatacct atttctgaat atcggaaaaa agcacgagtt tctttggaaa atcaatgggg
    37981 aatcaatact tggcttgttt tcttatctat aatatttacg agcgtcatcc agaacttttt
    38041 tgacggtttc agtccttttc cggaagaaag catgcagcag cacttattga gttttctagt
    38101 cgataattta cttttattcg cattcaacta tgcattgttt tacattgcgc tagtagttgt
    38161 acggggtggc agaccaaaaa gtaacctttt attttcagtt ttccaaagaa agtattttgg
    38221 ttcgatactt ggcatcaatt tgatcaattc agtcatcaac tggctactca acagtttaat
    38281 ctttctgccc atgtttttaa tcagcggagt gagtacttat atgcagctgc ttttcagttc
    38341 aaatgcggta cctacgggtg tcatgccaga agattttctt gatatcagct ttgttttatc
    38401 gatgataggg atgatttttg tttcagcact catcatgagt attgttggcg gtttattcca
    38461 atttgctgca tggacaaagt tcgactatcc tgatttgtcg gttattcaat gtttgaaata
    38521 tggctggttt ttattgaaag atagaattgg tacgtatatt ttgttgcagc tgtcttttat
    38581 cggctggtat ttgctaggag caatcgcttt gttcatcggt cttttgtggg tcattgttta
    38641 tgtgaatgtg tcagttgcag aattttatga acaagcacgg attgaaaaag gaggtccaga
    38701 cgcctatttt acttagcatg taagcagttt attcatcaaa aaaataactt ttgtggtagg
    38761 ataaatcttt gagggggaat gtacgatgaa attaactgtt ttaggatgct taggagcgta
    38821 tccttataaa aatcaaggga caacaggtta tttgcttcaa tctgacgggt tcaatttgtt
    38881 gatcgatgca ggtagtgcta cgctgatcaa attacaagaa caccttgatc cacttgatct
    38941 agatgctgtg atccttagcc attatcatca tgatcatatt gctgaccttg gtgtattaca
    39001 gtattactgg caacttcatc ctaagagaaa tccggagaca ttgctttcaa tttatggaca
    39061 cacaaaagat tccattcact ttgaagaatt gacgttggaa aatgtctcaa aaggacatcc
    39121 ttattttgaa gctgaagaat taaaaattgg tccttttctg attactttta tggaaacaat
    39181 ccatccagta gtatgttacg ccatgcgtat cgtagaagaa acaacgggga aagtattcgt
    39241 atttacaggt gattcaggtt atttggaaag tttcgctgat tttgcgaaag atgctgattt
    39301 gtttctagca gatacctatc tttttgaagg aaatgaacat catcatgccc atttcacttc
    39361 aaaagaagct ggagaacttg cgaaatcagc taacgtaaag aaacttgtac ttacacacct
    39421 acctcagttt ggtgatttag aacaattgaa aaatgaagcg caaaaggcag caggcaaagg
    39481 gattgatata gaacttgctg cagttgacaa agtgtttaac atttaggagg aagaacatga
    39541 tttttgtacc aaatgaaaac cacgacccaa gaatcaattt agcaattgaa caatatttgc
    39601 tgcaagaaat gcctttagat gaacctattt tgctctttta tatcaatgaa ccttcaatca
    39661 tcatcggcag aaaccaaaat acgcttgaag aaatcaatcg agattatgtg gaagaaaatg
    39721 gcattcatgt tgtccgtcgc ttaagtggag gcggagctgt ctaccatgat tttggaaacc
    39781 tgaactttag ttttatcatg cctgatgatg gggattcatt ccgtgacttt gcgaaagtaa
    39841 cacagccaat catccaagca cttcatgaat tgggtgttgc tggggcggaa ttgaaaggcc
    39901 gaaatgatct agtcattgat ggcatgaaat tttctggcaa cgcgatgtat gctaccaacg
    39961 gtcggatgtt tgctcatgga acaattatgt tcgactctga tatcaatgaa gtagtcaatg
    40021 cattaaaagt aaaaaaagac aaaatcgaat caaaaggaat caaatcgatc cgttctcgtg
    40081 taacgaatat caaaccattt cttccagaag aaaagcaaaa aatgacaaca gaagaatttc
    40141 gtcaagaaat cttgttgaaa attttcggcg tagagtcagt agaagaagta aaaacgtacg
    40201 aattgactga tgaagattgg aaaaaaatca atcgaatctc tgaggaatat tatcgaaatt
    40261 gggattggaa ttacgggaag tctccagatt tcaatttctc tcgccaaaaa cgtttttcta
    40321 tcggttcgat tgaagtccat ttgaatgtct cagaaggaat gatcaaagaa gcaaaaatct
    40381 ttggcgattt cttcggacta ggagatatct ctgacgtgga aaaaaaattg gttggacaga
    40441 aatatgataa agcatctcta tcaaaaatag tcagccaaat tgatgtgaag aaatattttg
    40501 gtgcgataga accagaagaa ttgcttacct tactttattg aaaataaaaa atagcggaat
    40561 ccacatatat ggattccgct attt
//
LOCUS       DAHUVV010000015        40446 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.015, whole genome shotgun sequence.
ACCESSION   DAHUVV010000015 DAHUVV010000000
VERSION     DAHUVV010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.015"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            165..1181
                     /locus_tag="NK767_000799"
     CDS             165..1181
                     /locus_tag="NK767_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCH6693812.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            1322..2623
                     /locus_tag="NK767_000800"
     CDS             1322..2623
                     /locus_tag="NK767_000800"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCH6693813.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            2669..3331
                     /gene="deoC"
                     /locus_tag="NK767_000801"
     CDS             2669..3331
                     /gene="deoC"
                     /locus_tag="NK767_000801"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCH6693814.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            3352..3750
                     /locus_tag="NK767_000802"
     CDS             3352..3750
                     /locus_tag="NK767_000802"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCH6693815.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            3816..4898
                     /locus_tag="NK767_000803"
     CDS             3816..4898
                     /locus_tag="NK767_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCH6693816.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            5110..6672
                     /locus_tag="NK767_000804"
     CDS             5110..6672
                     /locus_tag="NK767_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6693817.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            6665..7798
                     /locus_tag="NK767_000805"
     CDS             6665..7798
                     /locus_tag="NK767_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6693818.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            7798..8754
                     /locus_tag="NK767_000806"
     CDS             7798..8754
                     /locus_tag="NK767_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6693819.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            8853..10019
                     /gene="deoB"
                     /locus_tag="NK767_000807"
     CDS             8853..10019
                     /gene="deoB"
                     /locus_tag="NK767_000807"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCH6693820.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            10043..10861
                     /locus_tag="NK767_000808"
     CDS             10043..10861
                     /locus_tag="NK767_000808"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCH6693821.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            10878..11585
                     /gene="deoD"
                     /locus_tag="NK767_000809"
     CDS             10878..11585
                     /gene="deoD"
                     /locus_tag="NK767_000809"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCH6693822.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(11883..12569)
                     /gene="gpmA"
                     /locus_tag="NK767_000810"
     CDS             complement(11883..12569)
                     /gene="gpmA"
                     /locus_tag="NK767_000810"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCH6693823.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(12741..13322)
                     /locus_tag="NK767_000811"
     CDS             complement(12741..13322)
                     /locus_tag="NK767_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCH6693824.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(13455..14138)
                     /gene="rpiA"
                     /locus_tag="NK767_000812"
     CDS             complement(13455..14138)
                     /gene="rpiA"
                     /locus_tag="NK767_000812"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCH6693825.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            14457..14870
                     /gene="rpsL"
                     /locus_tag="NK767_000813"
     CDS             14457..14870
                     /gene="rpsL"
                     /locus_tag="NK767_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCH6693826.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            14954..15424
                     /gene="rpsG"
                     /locus_tag="NK767_000814"
     CDS             14954..15424
                     /gene="rpsG"
                     /locus_tag="NK767_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCH6693827.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            15518..17605
                     /gene="fusA"
                     /locus_tag="NK767_000815"
     CDS             15518..17605
                     /gene="fusA"
                     /locus_tag="NK767_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCH6693828.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            17777..18964
                     /gene="tuf"
                     /locus_tag="NK767_000816"
     CDS             17777..18964
                     /gene="tuf"
                     /locus_tag="NK767_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCH6693829.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            19371..19679
                     /gene="rpsJ"
                     /locus_tag="NK767_000817"
     CDS             19371..19679
                     /gene="rpsJ"
                     /locus_tag="NK767_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCH6693830.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            19710..20339
                     /gene="rplC"
                     /locus_tag="NK767_000818"
     CDS             19710..20339
                     /gene="rplC"
                     /locus_tag="NK767_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCH6693831.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            20367..20990
                     /gene="rplD"
                     /locus_tag="NK767_000819"
     CDS             20367..20990
                     /gene="rplD"
                     /locus_tag="NK767_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCH6693832.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            20990..21280
                     /gene="rplW"
                     /locus_tag="NK767_000820"
     CDS             20990..21280
                     /gene="rplW"
                     /locus_tag="NK767_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCH6693833.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            21319..22152
                     /gene="rplB"
                     /locus_tag="NK767_000821"
     CDS             21319..22152
                     /gene="rplB"
                     /locus_tag="NK767_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCH6693834.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNRAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            22195..22473
                     /gene="rpsS"
                     /locus_tag="NK767_000822"
     CDS             22195..22473
                     /gene="rpsS"
                     /locus_tag="NK767_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCH6693835.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            22496..22843
                     /gene="rplV"
                     /locus_tag="NK767_000823"
     CDS             22496..22843
                     /gene="rplV"
                     /locus_tag="NK767_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCH6693836.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            22857..23513
                     /gene="rpsC"
                     /locus_tag="NK767_000824"
     CDS             22857..23513
                     /gene="rpsC"
                     /locus_tag="NK767_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCH6693837.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            23516..23950
                     /gene="rplP"
                     /locus_tag="NK767_000825"
     CDS             23516..23950
                     /gene="rplP"
                     /locus_tag="NK767_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCH6693838.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            23940..24128
                     /gene="rpmC"
                     /locus_tag="NK767_000826"
     CDS             23940..24128
                     /gene="rpmC"
                     /locus_tag="NK767_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCH6693839.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            24153..24419
                     /gene="rpsQ"
                     /locus_tag="NK767_000827"
     CDS             24153..24419
                     /gene="rpsQ"
                     /locus_tag="NK767_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCH6693840.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            24477..24845
                     /gene="rplN"
                     /locus_tag="NK767_000828"
     CDS             24477..24845
                     /gene="rplN"
                     /locus_tag="NK767_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCH6693841.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            24882..25190
                     /gene="rplX"
                     /locus_tag="NK767_000829"
     CDS             24882..25190
                     /gene="rplX"
                     /locus_tag="NK767_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCH6693842.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            25218..25757
                     /gene="rplE"
                     /locus_tag="NK767_000830"
     CDS             25218..25757
                     /gene="rplE"
                     /locus_tag="NK767_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCH6693843.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            25776..25961
                     /locus_tag="NK767_000831"
     CDS             25776..25961
                     /locus_tag="NK767_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCH6693844.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            25997..26395
                     /gene="rpsH"
                     /locus_tag="NK767_000832"
     CDS             25997..26395
                     /gene="rpsH"
                     /locus_tag="NK767_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCH6693845.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            26427..26963
                     /gene="rplF"
                     /locus_tag="NK767_000833"
     CDS             26427..26963
                     /gene="rplF"
                     /locus_tag="NK767_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCH6693846.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            27124..27480
                     /gene="rplR"
                     /locus_tag="NK767_000834"
     CDS             27124..27480
                     /gene="rplR"
                     /locus_tag="NK767_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCH6693847.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            27501..28001
                     /gene="rpsE"
                     /locus_tag="NK767_000835"
     CDS             27501..28001
                     /gene="rpsE"
                     /locus_tag="NK767_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCH6693848.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            28016..28195
                     /gene="rpmD"
                     /locus_tag="NK767_000836"
     CDS             28016..28195
                     /gene="rpmD"
                     /locus_tag="NK767_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCH6693849.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            28239..28679
                     /gene="rplO"
                     /locus_tag="NK767_000837"
     CDS             28239..28679
                     /gene="rplO"
                     /locus_tag="NK767_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCH6693850.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            28679..29974
                     /gene="secY"
                     /locus_tag="NK767_000838"
     CDS             28679..29974
                     /gene="secY"
                     /locus_tag="NK767_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCH6693851.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            30036..30683
                     /locus_tag="NK767_000839"
     CDS             30036..30683
                     /locus_tag="NK767_000839"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCH6693852.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            30874..31092
                     /gene="infA"
                     /locus_tag="NK767_000840"
     CDS             30874..31092
                     /gene="infA"
                     /locus_tag="NK767_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCH6693853.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            31125..31241
                     /gene="rpmJ"
                     /locus_tag="NK767_000841"
     CDS             31125..31241
                     /gene="rpmJ"
                     /locus_tag="NK767_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCH6693854.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            31259..31624
                     /gene="rpsM"
                     /locus_tag="NK767_000842"
     CDS             31259..31624
                     /gene="rpsM"
                     /locus_tag="NK767_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCH6693855.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            31652..32041
                     /gene="rpsK"
                     /locus_tag="NK767_000843"
     CDS             31652..32041
                     /gene="rpsK"
                     /locus_tag="NK767_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCH6693856.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            32121..33059
                     /locus_tag="NK767_000844"
     CDS             32121..33059
                     /locus_tag="NK767_000844"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCH6693857.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            33087..33467
                     /gene="rplQ"
                     /locus_tag="NK767_000845"
     CDS             33087..33467
                     /gene="rplQ"
                     /locus_tag="NK767_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCH6693858.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            33739..34578
                     /locus_tag="NK767_000846"
     CDS             33739..34578
                     /locus_tag="NK767_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCH6693859.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            34554..35423
                     /locus_tag="NK767_000847"
     CDS             34554..35423
                     /locus_tag="NK767_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCH6693860.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            35413..36210
                     /locus_tag="NK767_000848"
     CDS             35413..36210
                     /locus_tag="NK767_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCH6693861.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            36413..37159
                     /gene="truA"
                     /locus_tag="NK767_000849"
     CDS             36413..37159
                     /gene="truA"
                     /locus_tag="NK767_000849"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCH6693862.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(37614..40283)
                     /locus_tag="NK767_000850"
     CDS             complement(37614..40283)
                     /locus_tag="NK767_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HCH6693863.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        13035 a         7426 c         8832 g        11153 t
ORIGIN      
        1 ttttttacga taaaataaat aagatacaga acttgtatag aaggctgcca agtcggtaga
       61 taaaggaggt ttttatcaaa aaacatactt tccttttttc tctattcttt tcttgggggt
      121 tttggtacac tgatagggaa tttagataga agggagccaa taggatgaag ctgaccatca
      181 gagatatcgc agaaatggca ggcgtatcag taacaactgt ctcacagatt ttgaataaca
      241 aaggaagcag atttagtgat aagacaagaa aaaaagtgtt gtcgatcgtg aatgaacatc
      301 attataagcc cgattatttc gcttcaaatg tgatcaatcg acattcaaag accatcggaa
      361 tgatcgtccc tgatgtcact gatttcttct tctccaaact gatcgaaggt gtagagagct
      421 atttgaatcc tttgggctat gtgatcatgt tatgcaattc tcgtcatagc caagaaaatg
      481 agatcaagta tttacaagag ctttcccacc gctcagtgga cggaatcttg ctggccacac
      541 caaatatttt acctgagaaa tatgcattag acagcggttt ttatcaaaaa atgccaatca
      601 ttttaatcga tcgcggattg aatcgtcggg ataacggacg gcttatcgta aaggaatatg
      661 agggcgccta tcaagctgtt tcgctgttta ttgaaaatgg ccatacgaag atcggcatgc
      721 tgaaggaaac cactggatac tatcaattag aagaacgttt caacggatat cgtcatgcgc
      781 tgaaagatca tggcctgcct tttaatggga aatttgttga acatggagag ttgactgtcc
      841 aaggtggata tactgcatca aaaaaattac tcaaacataa agatatcacg gctatttttt
      901 gcgggaatga tgccatggcg atcggatgct atcaggcgat cgatgaattg ggcaaaaaaa
      961 tcccagaaga tatctcagtc ataggattcg atggattaaa gctgtcagaa tacatgattc
     1021 cgcaattgac gacagtgcag cagccaagtt ttgatattgg attttatgct gcacgtttcc
     1081 tgatcgatac gatcgaattt cctcaaagaa aagttccgaa caaagtgttc gaaacaaaat
     1141 taattattcg tgagagcgta aaagtattga caaagggcta actcggcggt atattgctta
     1201 tgtaagcatt tacaaaggct agttagtgct ttttttattt ttagtaaacc agtttactaa
     1261 agtggtttac ctttcctaaa aataatgtat aataccgaat gaagacaagg aggatcaggc
     1321 aatgcgaatg gtggatctga ttgaaaaaaa gagggatgga aacgagctgt cgaaagagga
     1381 aatcgagtac atcgtcacga actatacgaa tggaaagatt ccagattatc aagtcagtgc
     1441 attattgatg gcaatttttt atcaagacat gacaaatgaa gaaatcacga acttaacatt
     1501 ggcgattgct aattctggtg atgtcatcga tttgagttct cttgaaggaa taaaggtaga
     1561 taagcattcg acaggtggcg ttggtgacac gaccacattg attttagccc ccctagtagc
     1621 tagtgtcgga gtgacagtgg ctaaaatgtc tggacgcggt ctaggttaca caggtggtac
     1681 actagacaag ctagaagcaa ttcccggatt tcagatcgag ctttcggatg aagaatttgt
     1741 acgcattgtt aatgaaagta aagtagcggt aattggtcaa tcaggaaact tggcaccggc
     1801 ggacaaaaaa ttgtatgcct tgcgtgacgt cacagcgacc gttgattctc ttccgctgat
     1861 tgccagttcg attatgagta aaaaaatcgc ggcaggagca gatgcaatcg ttttggatgt
     1921 gaccactgga gacggtgctt tcatgaagaa catcgaagat gcaagacgtc tggcaaagac
     1981 catgacgagc attgggaaac tggctaaccg agaaacagtg gcggttatct cagatatgtc
     2041 agaacctctt ggcgaagcta tcggaaatag tctagaagtt gtcgaggcaa tcgaaacatt
     2101 acaaggaaat ggtccagaag atctagtaga aatgtgctac gccttaggaa gccaaatggt
     2161 tgttcttgct ggcaaagcta agacaaccga tgaagcaaga acgttgcttc aagaagctct
     2221 ggaatcaggt aaagcgttag caaaattcaa agaaatgatc caaaatcaag ggggagaccc
     2281 agcgatcgtg gaacatccag aacgaatttt gactgcacgc tacacaatgg agcttcctgc
     2341 taaacaaagc ggagttgtgt caaaaatcgt ggcaaatgaa ttaggaattg ctgcaatgat
     2401 gctaggtgct gggcgtaaaa ccaaagaaga agatattgat cacgctgttg gattgaaact
     2461 gcataaaaaa attggtgaca ctgtgacaaa aggtgagtca ctattgacga tctatagtaa
     2521 cgacgaagaa atcacttctg tcattgaatt attatataaa aatattgaaa ttggtgaatc
     2581 ggcaatgaag ccaacgttga ttcatgacat catcacagaa taaaggaaaa aagcggactt
     2641 taccgtttta aagaaaaggg gaaaagaaat ggaactaaac cgaatgatcg accatactat
     2701 tttgaaagca gacgcatcta aagaagctgt gatgcaaatt atcgaagaag caaaaaaata
     2761 ccatttttat tctgtctgca tcaatccagc atgggttgcg ctagcgaaag aacaattaca
     2821 aggtgagcca gtagccgtat gtacagtaat cgggtttcct ctaggagcaa acacacccga
     2881 gacaaaagca tttgaaacaa cagatgccat caataacggt gcagatgaag tcgatatggt
     2941 catcaatatc ggtgctttga aatcaaagga tgatcaacaa gtccaaaaag atatcgaagc
     3001 agttgttgaa gcagcaaaag atcgtgcgct tgtcaaagtt attattgaaa catcgttatt
     3061 agatagagaa gaaatcatcc gagcttgcga aatagcaaaa gctgcaggcg ctgattttgt
     3121 caaaacatca actggtttct caacaggcgg agcaaaggta gaggatgtcc gtttgatgcg
     3181 tgaaactgta ggaccagaaa tgggtgtcaa agcttccggc ggtatccata acgaagaaga
     3241 agcaatggca atgatcgaag ctggtgctac aagaatcgga acaagcgcag gagtagcaat
     3301 cgtatctggt tcaactggtg aaggctatta attttttcaa aggagaagct catggcagaa
     3361 ctcaaacaag aatggatcaa cacagctatt gaagcgttag ataaagccta tgttccttat
     3421 tcccattttc cggtaggcgc ttgtctcgta acggaatcag gcaagattta tcaaggcatc
     3481 aacattgaga atgcatcatt tggattaacg aattgtgccg agcgcacagc ttttttcaaa
     3541 gcagtatctg aaggagaaaa aacatttacg catctagtcg tagcggggca cacccccgat
     3601 cctatctctc cttgtggtgc atgtcgacaa gtaatggcag aattctgttc ccctgatatg
     3661 ccggttacgc tagtaggaga caatggtgtg acgaaggcaa cgaccgttcg tgcactgctg
     3721 ccttatgcat ttactgaaaa agatctataa ctcatgttca ttcagtcagc tgactgttga
     3781 aaaataaaac aattatttta ggaggcttta cagaaatgaa aaaagcaaaa ttatttggtt
     3841 taggtgctgt cgcactagca gcagggttat ttttaggtgc atgcggcaac aacggctcta
     3901 ctgattcatc cggtggcaaa agctcatcag acacaacaac tgcagcattg atcacagaca
     3961 caggtggtgt agacgaccgt tcattcaacc agtcagcttg ggaaggacta gaaaaatggg
     4021 ggaaagatca aggactttca cgaggaaatg atggcttcca atatttccaa tcttcaaatg
     4081 aatcagatta catcccaaat attgaccaag cattgaatgc tggattcaaa acgatttttg
     4141 gtattggtta taaactaaaa ccagcgatcc aagaacaagc aacaaacaat acaggtacga
     4201 actttgttat tatcgatgat gtcatcgatg gattagataa cgttgtatct gcaactttca
     4261 aagacaacga agcttcttat cttgcaggtg ttgcagcagc ttatacgact gaaacaaacg
     4321 tagtcggctt tatcggcggt gtaaaaggtg aagtaatcga tcgttttgat gctggattca
     4381 aagccggtgt tgatgcaggt gcgaaagaat taggaaaaga aatcaaagta ttgaatcaat
     4441 atgctggtga cttctctgct ccagataaag gacgttcaat tgctcaaggt atgtatgcac
     4501 aaaacgcaga tatcatcttc catgcttcag gcggtacagg taacggtgta ttccaagaag
     4561 caaaatcatt gaacgaatca ggcgacaaaa aagtttgggt tatcggtgtt gaccgcgacc
     4621 aatcagacga aggcgaatac acattaaacg gcgaaaagaa aaacttcaca ttgacatcta
     4681 cactgaaagc agttggaaca gtagtagaag atttggcaca aaaatcagcg gacggtaaat
     4741 tccctggcgg cgaacacact gtttatggat tgaaagaaga cggtgttggt ctaacagaag
     4801 gacaactttc tgacgaagcg aaaaaagcag ttgatgaagc aaaagaaaaa atcatttcag
     4861 gtgacgtaaa agttccagaa actcctgaag aaaactaaaa ccatttctat agccttgtta
     4921 gacacatgtt tgacaaggct atggtttgat tagaacaaga attgttcatt tatccaagct
     4981 gccttgtgac agtttggata agggaacaac tccctgaagt aatgtttgtt tcaaaagaag
     5041 aacgctcact aagcgtgaat cttagtcaat cgttgcccta gatagaaatc gttaggatgt
     5101 gaaaaaaagg tggttcaaga aaatattgtc atagaaatgc agaatattac caaagaattt
     5161 ggaacattca aggcaaatga caacatcaat ttacaagtaa aggcaggaga aattcatgcg
     5221 ttgcttggag aaaacggtgc tggcaaatct acattgatga acgtgctttc cggattatta
     5281 gagccgacat cagggaaaat tttgatgcgt gggaaagaag taaagatcac aagtccgacc
     5341 aaagccaacc aattagggat tgggatggtc catcagcact ttatgcttgt tgatgccttt
     5401 actgtaacag aaaacatcgt gttgggaagc gagcctagtc gcgcagggat gcttgaccat
     5461 aaaaaagcac gaaaagagat ccaaaaagtt tctgaacagt atggattgtc agtcaaccca
     5521 gatgcttacg ttcgtgatat ttcagttgga atggaacaac gggtagaaat tttaaaaaca
     5581 ctttaccgag gagcagatgt actgattttt gatgagccga cagctgtatt gacccctcaa
     5641 gaaattgatg aattaatcgt gatcatgaag gaattagtca aagaaggcaa gtcgatcatt
     5701 ttgattacgc ataagttaga tgaaatcaaa gcagtagctg accgctgtac agttatccgc
     5761 cgtggaaaag gaatcggtac agtcaacgtt aaagacgtaa cctcacaaca attagccgat
     5821 atgatggttg gaagaacggt ttcattcaaa acgatgaaaa aagaagcgaa gcctcaagaa
     5881 gtcgttttgt ctattgaaaa tctagtggtg aaagaaaatc gtggattaga agccgtgaaa
     5941 aacttgaact tagaggttcg tgctggcgaa gtacttggta tcgctggaat cgatggaaac
     6001 gggcagtcgg agttgatcca agctttgact ggtttgcgaa aggcagaaag cggacatatc
     6061 aagctaaaag gggaagacat cactaataaa aaaccccgaa aaatcactga acatggtgta
     6121 ggacacgtgc cagaagaccg tcataaatac gggttagtcc tagatatgac attatctgaa
     6181 aatatcgcac tacaaaccta tcatcaaaaa ccttacagta aaaacggcat gctgaattat
     6241 tcagtaatga atgaacatgc cagagaattg atcgaagaat atgatgttcg aacaacgaat
     6301 gaacttgttc ccgcaaaagc tttatcaggc ggaaatcagc aaaaagcaat tatcgctcgt
     6361 gaagtcgacc gagatcctga tctgttgatt gttgcaaatc caactcgtgg actggatgta
     6421 ggtgcgatcg aatttattca taaacgtctg atcgaacaaa gggacaaata caaagcagtg
     6481 ttactgatca gtttcgaatt agaagaaatt ttaaatgttt cggatcgtat tgctgttatc
     6541 catgaaggag aaattgtcgg tatcgttgat ccgaaagaaa catctgaaaa tgaattaggt
     6601 ctattgatgg cgggctatac cttagaagaa gcacgaaaag agttagaaaa gggggctggc
     6661 gcacatgaat gatcgcagcg aaaaattccg taatttatta gtgcctgtac tttcagttgt
     6721 tttaggattg atcttaggcg cgatattgat ggctgctttt ggttttcagc ccatccaagg
     6781 ttattcttct atgctagatg cagcccttgg ttcgcaaaga gggataggtg aaacgttaag
     6841 gcaagcaacg ccgttgattt ttacagcatt aggtttttct gtagctaact cagctgggtt
     6901 tttcaatatt gggctttctg gacaagcact ttgcgggtgg atcgtcagtg tctgggtggc
     6961 acttgcgttt ccagatctgc caaaagttgt tttgcttccg ttatgcgtga tcttaggtgc
     7021 cttagctggt gcgatggcag cggctgttcc aggtttactt cgagcatttt ttggaacaag
     7081 tgaagtgatc gtaacaatca tgatgaacta tatcttatta tacgtaagca catttgcttt
     7141 acatgaagtg atgcctgaaa gctatcgctc aagtttggac tcttccaata tgatcagtca
     7201 aaatgcctca ctgcggttac catggttaac acaagctttt ggcggctctc gcgtaaactc
     7261 aggattattt ttagcaatca tcgcgcttat tttcgtttgg ctgctgatga agaaaacgac
     7321 tttaggattt gaaatccgtt ctgttggatt gaatcctttt gcttctgagt atgcaggaat
     7381 gagtagtaaa cgtacgatcg ttttgtcgat ggtcatttct ggcgctttgg cagggcttgg
     7441 cggtgtggtt gaaggattag gaacgtatca aaacttcttt gttcaaacga cttcgctatc
     7501 tattggattt gacggaatgg ctgtttcctt gcttggttct ggatcagcaa tcggtattct
     7561 tttatcagcg atcttgttca gtattttgaa gatcggtgga cttggcatgc agacaggagc
     7621 aggtgttccg tttgagatcg tcaatgtatc gattgctttg attatcttct ttgtcgggat
     7681 caacttcctg attcgtttcg tcatggctaa agtcttccaa gggaaaaaac aagaagagat
     7741 cgttgcgaca atagaatcga aaccaacgaa tcaaaaccat gaagggggcg aactataatg
     7801 tcgactatcg aattaattgc cttgatcctt acttctacac tggtttattc aacaccgcta
     7861 attctaacgt cactaggcgg aactttttct gaacgaagcg ggatcgtaaa cgtagggcta
     7921 gagggaatca tggtcatggg tgcttttagt tccatcgttt tcaacttgac gatgagtcca
     7981 tcacttggtt ctatgacacc atggatcgct attttagtgg gtggcgtcgt aggtatccta
     8041 ttttctctga ttcacgcagt agcaacagtc agtttgcgtg ctgaccatat tatcagcggg
     8101 acagtaatta atctgatggc acctgcttta ggtgttttct tgatcaaagc atggtatggt
     8161 aaaggtcaaa ctgataacat cacgcagaat ctggggtatt tttctttccc aggattagca
     8221 gatattccgg tcattggacc aattttcttt aaaaatacat tcttacctgc ttattgtgct
     8281 attttgattg ctgtcctagc ttgggtgatc atcttcaaga cgaaattcgg cttgcgcctc
     8341 cgttcagttg gtgaaaatcc tcaagctgcc gacacattag gtatcaatgt atatgctatg
     8401 cgttattcag gcgtactgat ttctggcttg ttaggcggta tgggcggtgc tgtttttgct
     8461 cagtcgattt ccggtaactt ttcagctggt acgatcgttg gacaagggtt catctccatg
     8521 gctgctatga tctttggtaa gtggaatcct ttaggggcaa tgggtgcctc attgttcttt
     8581 ggttttgctc aaagcttgag tatcattggc gcacaattac cagttatttc ttcgattccg
     8641 ccagtattgc tgcaaattgc tccatatttg ctgacaatca ttgtgctggt tgttttctta
     8701 gggaaagctt ctggtccaaa agcaaacggg aagaactata tcaaatcaaa ataatctttt
     8761 agcgaataaa aagggtgggt tacgggaagt gtttttcgtc tcgccctttt tgttttaaag
     8821 gcaataaaaa taaaaagaaa gaaggaataa tcatgtttaa acgcgtacat ctgatcgtca
     8881 tggattcagt aggaattgga gaagcaccag atgccgataa gttcggtgat gttggttctg
     8941 atacattagg acacattgcg aaagaagcag gattgaacgt tccacattta gagcagcttg
     9001 gtttaggaac gatccgtcct cttgaaggtg tggaatcagt gaaagatcat caaggctatg
     9061 cgacgaaatt agaagaagtt tctgtaggga aagatacgat gacaggccac tgggaaatca
     9121 tgggattgaa tatcaaaact ccatttcgtg tgtttcctaa tggattccct gatgatctga
     9181 tcaaacaaat cgaagaattt tctggccgta aagtcgtatg taacaaacct tatagcggaa
     9241 cagctgttat tgacgattac ggtgaacacc aaatgaaaac aggcgattta attgtatata
     9301 cttctgccga tcctgtcctt cagatcgcgg cacatgaaga aatcattcct ttagaagaat
     9361 tatatcggat ttgccaattc gttcgtgata taacaaaaga tgatccgtat atgattggcc
     9421 gaatcattgc acgtccgtac gtgggtgaac caggagactt cacacgaaca agcaatcgcc
     9481 atgattatgc gttgaatcca ttcggacaca cagtccttga ttctttgaaa gaagcaggaa
     9541 aggacgtgat tgctgttgga aagatcaacg atattttcaa cggacaagga atcactgaat
     9601 ctgtccgcac gaaaagcaat atggatggcg ttgaccagct gctgaaagtt atgaaaaacg
     9661 atttttcagg aatcagcttt accaatttag tagattttga tgcattatac ggtcatcgtc
     9721 gggatgtagt cggctatgcg catgcgattg aagaatttga cttacgaatt ccagaattgc
     9781 ttgaagcgat ggaagaagac gacttgctaa tgattacagc cgatcacgga aacgatccga
     9841 cgtttactgg taccgatcat acgcgagaat atgtaccgtt attggtttac agtaaaaaaa
     9901 tgaaagaaca aggggcattg cctcaaggat attattcaga tatttcagca acgattgctg
     9961 agaactttag tgtttcttcg actgaaaatg gcgaaagctt cttgaaatta ttaaaataat
    10021 ctttttaaat aggagaaagc aaatgaccaa gttaagcgag atgttacaag aaacgaccca
    10081 gtttatcctt gataaaggag taaaagaaat tgattttggt ttgattctag gttcaggatt
    10141 aggtgaatta gcagatgaaa tcgaagatgc tgtaatgatt tcttatgatg agatcccgtt
    10201 ctttcctgtt tcgacagtag ttgggcatgc aggaaagcta gtttatgggt ctctggcagg
    10261 caagaaagtg ttagccatgc aagggagatt ccactactat gaaggtcatt ccatgcaaac
    10321 agtgacctat ccagtacgtg tgatggccgg actaggggcc cattcagtca ttgtgactaa
    10381 tgcatgtggt ggtgtcaacg aatcatttgt accaggagat ttgatgctga ttaccgatca
    10441 tatcaatttt actggtgaaa atcctttgat cggtccgaat gaagatgcaa tgggccctag
    10501 atttccagat atgagtcaag cttatacgcc tgactatcaa gcaacagcaa aagcagcagc
    10561 agaaaaacta ggtttggatc ttaaagaagg cgtatatatg ggctattctg gaccaactta
    10621 tgaaacgcct gcggagatcc gaatgtctcg gacaatagga gccgatgcag tagggatgtc
    10681 gactgttcca gaagtcatcg tagcagctca cagcggatta aaagtgttag gaatctcttg
    10741 tattaccaac ttagcagcag gtatgcaagc gaatctgagc catgaagaag ttgtagaaac
    10801 aactcagaga gtcaaacaat cttttaaagc attggttaaa gaaacattga cattattgta
    10861 ataggaggaa caataaaatg agtgtgcata ttgaagcgaa agaaggcgag atcgccgata
    10921 agattttact tccgggagat cctttgcgag ctaaatatat tgcggagact tttttggaag
    10981 atccagtttg ttacaatcag gtacgcggta tgttaggctt taccggcaaa tataaaggag
    11041 aacgtatttc tgttcaagga acaggaatgg gaatgccatc agccaccatc tatgcccatg
    11101 aattgatcca gtcgtatggt gtaaagaaat tgattcgagt aggtacatgc ggtgcattgt
    11161 caaaagacgt ccatgttcgt gatttagtac tagcacaagg tgcagcgacc agttcttcga
    11221 tgatcgaaaa aaatttccaa gcctttcatt ttcctccaat cagtgatttc aatctattac
    11281 tgaaagcata tgagatagct aaagaaaagg gctatacggt tcatgtagga aatgttcttt
    11341 cggaagattc tttctacaaa gatgatttga cagagacgtt ccaattagct gaacttggcg
    11401 ttttaggagt ggaaatggaa gcagcggctc tttattactt aggcgcgaaa taccatgtgc
    11461 agacattaag tttgatgacc gttagcgacc atttgatcac cggtgaagaa acaacagctg
    11521 cagaacgtca atctacgttc aatgaaatga tcgaagtagg attggaaaca gcgatagcag
    11581 aataagaagg aaatgaaaaa gctgccctgc attaagtaat aagaacatcc aagcaaaaat
    11641 agctcctcat gtttttgact tggctggact tattattgca aatgtaagct tttgaacacc
    11701 gtttaccaag aggttgtgaa tctgccgttt aactctgagt cacaaggaac aatttcaaaa
    11761 aacagctttt catgtttgat gaatattgtg acttgtgacc gagaagttgg caggagaaaa
    11821 ccgtttatac gaaaaaaggg actgtgacaa atcgtgtcac agtccctttt ttatagtgtt
    11881 ttttataggt agtatttttt agcaactgtt aagtcatcat ttaattcata aacaagtggt
    11941 tgacctgttg gaatttcaag atccatgatg tcttcgtcag agataccttc gatatgttta
    12001 gctagggcgc gtaatgagtt accatgtgca gctactaaaa cagttttgtt gtctagtaat
    12061 gctggagcaa tttcatcttg ccagaatggc aaagcgcgtt ctaatgtcac tttcaagttt
    12121 tctccaccag gaatatcgcg tttgtctaac attgcgtaac gacggtcatt tgctgcagaa
    12181 ccttcgtcag tttcttccat taatggagga agagtatcgt aagaacgacg ccagatgtgc
    12241 acttgttcgt cgccgtattt ttcagctgtt tcttttttgt tcaaaccttg taatttacca
    12301 tagtgacgtt cgtttaagcg ccatgatttg atttgtggta cccaaagttg atctgaatat
    12361 tctaagatca agttacatgt tttgattgca cgagttaaaa cagaagtgta agcaacatca
    12421 aattcgattc cagcttcttt gattttgcgt ccgccttctt ttgcttcttc gattccttct
    12481 ggtgctagat ttacatcagc ccaaccagta aattggttca atgcattcca ttcactaaga
    12541 ccgtgacgag aaaaaactaa ttttggcatg tttagctctc cttttaagat aggtttatat
    12601 ttctgaaatt ttcagttctg ttttattgta caattttttg gaagagattt ccatcgaaaa
    12661 attagataag attctatgag aaaacaagta gagaatataa aaaaacgaac agtgcccacg
    12721 caactgttcg tttttaggta ttagatgcat aattgctcga tttctttttc aggtaaattg
    12781 atatatttat aacatgttcc aactgaaaca ccgcattttg aagaaataaa ttggatcgtt
    12841 tctttttttt cataatagag acgtcggatt tttttgatcg tttttgcatc gatttttggt
    12901 cttccgccga tttttccttt tttacgagct tcatctaatc caactaatgt acgttctttg
    12961 atcaatgcgc actccatgtt tgctaggttt tccattaatt ggaaatagat ttttcccatt
    13021 tcgcgcgtat cgattccttc atcaagacta atcaagtgca gcccgcgatc tttgaataat
    13081 tgcgtgagtt ctgttaattg ccgcgtagat ttgcctaatc gatgaagttg gcaaatgact
    13141 agtgcatcgc cttgttgcat gtcagcgatc actgactcca aatgaacagc agtagaatca
    13201 ttttgttcca cttcatatgt ctcatggaaa atttcttgac aaccaaagtc agataattga
    13261 tcaacctgag ttgttaaatc aacgtcattt atggttgtgc gtgcataacc aataattttc
    13321 atggtgattc ctttctagca gtgattttct aactggatat aacagtaaaa atgtagcata
    13381 aagtacgtca aaaagatagt tttttcgcaa attttccgct gtcttttttt attggatgat
    13441 caagcagttt ttctttattt acgacggatt gcgtcaagaa tctcaacgcc tttttctgag
    13501 ccgacaacta ctttagaaac catgtttagg aaaagcccat gttctactac cccaacaagt
    13561 tcatctaaat aggtagctaa gctttctggc tgttcgatga cttctagatg cagatcaatg
    13621 atgtaatgac caccatctgt ggttaagact tctccgtttt cgtctgtacg caagacgggt
    13681 tggaatcttt tttcttcaaa aatatgaagt agctgttggg agccataagg gatcacttca
    13741 actgggagtg ggaacttccc aagtttatcg actaatttag aagaatcaac gatccaaatc
    13801 gtttcttttg agtacgtagc gactattttc tcgaaaagca aagcagcgcc gccgcctttg
    13861 atcccttgaa aatcagcact gatttcatca gcaccgtcta ttgtcaaatc gactacgggg
    13921 acttcatcaa tactctttaa cggtattcct aatttttccg cttgttcttt tgtcgcattt
    13981 gaagtagtaa ctcctgtgat acgtaaacct tcttcacgca tacggcgacc aatctcttct
    14041 accatataat aggcggttga accggtcccg agacctacga tcatcccgtc ctttacgaat
    14101 ttcgcggctt caatgccagc taattgtttt aaattcatct ttgcgtccac ctttttttag
    14161 tttgagcatc gtgctttcct cactatttta gcgtgattta cggtaaaaag ataccattct
    14221 tttttattcc aataaagctt tttcaaaaaa aattgcatct cttgttgaca tgttttttca
    14281 ttcgtgatat tctaacaaag gtgctttatc tacaggtttc tgtgaagaaa cgtgctgact
    14341 gtttatcaaa gcatatttga taaggatgca gacattgcat ttatttttta tcacaaaaaa
    14401 agaaccacct ggatgtgtgg aactagacgc aaatcgtggg aaggaggaaa caaggaatgc
    14461 ctacaattaa ccaattagta cgtaaacctc gtaaatcaaa ggtggaaaaa tctaattcac
    14521 ctgctttgaa caaaggatat aacagcttta agaaaactca aactaacgta aactcacctc
    14581 aaaaacgtgg ggtagcaact cgtgtgggta caatgacacc taaaaaaccg aactcagctt
    14641 tacgtaaata tgcacgtgtt cgtttgtcaa acttaattga agttactgct tatatcccag
    14701 ggattggtca taacttacaa gaacacagtg ttgtattatt acgcggtggt cgtgtaaaag
    14761 acttaccagg ggtacgttac catatcgttc gcggtgcttt agatactgct ggtgttaacg
    14821 accgtaaaca aagccgttct aaatacggaa ctaaaaaacc taaagcttaa ttttaattat
    14881 taaacaaatg aaacttgctt ctactatata gaagtaatta ctatgaacaa tatttcggaa
    14941 ggaggagtta cggatgccac gtaaaggtcc tgttgcaaaa cgtgatgttt taccagatcc
    15001 tatttataac tcaaaattag taactcgctt gatcaaccgt gtaatggttg acggaaaacg
    15061 cgggattgca gctaacatta tctataatgc ctttgacttg atcaaagaat ctacaggtaa
    15121 cgatccatta gaagtgtttg aacaagcaat gaaaaacgtt caacctgttc ttgaagttaa
    15181 agcacgtcgt gttgggggtt caaactacca agtaccagtt gaagttcgcc ctgaacgccg
    15241 cgttactcta ggtcttcgtt gggtagtaaa ctacgctcgc ttgcgcggtg aacacacaat
    15301 ggaagaacgc ttagcaaaag aaatcatgga tgctgcaaac aacacaggtg cttctgttaa
    15361 aaaacgtgaa gacactcata aaatggctga tgccaaccgt gcgtttgctc actatcgctg
    15421 gtaagattct ctctgtttgg tcggctttta taagtcgacc tcagatagtg tccttatttt
    15481 tagcaactag atagtaaaga gaggagaaac aaggagaatg accagagaat tttctttaca
    15541 aaacactcgt aatatcggta tcatggccca catcgatgct ggtaaaacga ctgctactga
    15601 acgtatcttg tactatactg gtaaaatcca taaaattggt gaaactcatg aaggagcttc
    15661 acaaatggac tggatggaac aagaacaaga acgtggtatt acgatcactt ctgctgctac
    15721 tacagcacaa tggaagggtc accgaatcaa catcatcgac acaccaggac acgtagactt
    15781 cacagttgaa gttgaacgtt cattgcgtgt acttgatggt gctgtaactg ttcttgatgc
    15841 acaatcaggt gttgaacctc aaactgaaac agtttggcgt caagcaacaa cttatggtgt
    15901 acctcgtatc gtatttgcaa acaaaatgga caagatcggt gctgacttct tatattcagt
    15961 atcaacttta catgaccgtt tgcaagcaaa cgctcaccca atccaattac caattggtgc
    16021 ggaagacgac ttcacaggaa ttatcgacct tgtaacaatg aaggctgaaa tgtatacaaa
    16081 cgatcttgga acagagatcg aagaaacaga aattcctgaa gaataccgcg aattagctga
    16141 agaatggcgc gaaaaattgg ttgaagctgt agctgaaact gatgaagaac ttactttgaa
    16201 atacttagaa ggtgaagaaa tcactgaagc tgagttaaaa gaaggtatcc gccgcgcaac
    16261 agttaacgta gaattctacc cagtactatg tggttcagca tttaagaaca aaggggttca
    16321 attactattg gacgctgttc ttgattactt gccatcaccg ttagatatcc cagctatcaa
    16381 agggattgac cctaaaactg atgaagaagt agaacgtcct gcagacgaca gcgctccttt
    16441 ctcagcttta gcatttaaag ttatgactga cccattcgta ggtcgtttaa ctttcttccg
    16501 tgtgtactct ggtgttttac aatcaggttc atacgtacaa aacgcaacaa aaggcaaacg
    16561 tgaacgtgtt ggtcgtatcc tacagatgca tgccaactct cgttcagaaa tctctgaagt
    16621 ttacgctgga gatatcgctg ctgctgttgg tttgaaagac acaactactg gggacacact
    16681 atgtgatgaa aagagtctag ttattcttga atccatggaa ttcccagaac cagttatcca
    16741 agtcgctatt gaacctaaat caaaagctga ccaagataaa atgggtgtgg ctttacaaaa
    16801 actatctgaa gaagatccta ctttccgtgc tgaaacaaac gtggaaacag gcgaaacaat
    16861 catcgctggt atgggtgaac ttcacttaga tatcatcgtt gaccgtatgc gtcgtgaatt
    16921 caaagttgaa gctaacgtag gtgcgcctca agtatcttac cgtgaaactt tccgtgctgg
    16981 cactcaagcc gaaggtaagt ttgtacgtca gtctggtggt aaaggtcaat acggtcacgt
    17041 atggattgaa tttacaccaa atgaagaagg tgctggtttc gaatttgaaa atgcgatcgt
    17101 cggtggggtt gttcctcgtg aatacattcc agctgttgaa actggtttga aagatgctat
    17161 ggaaaatggt gtcttagctg gctatccatt agtagatatc aaagctaaac tttacgatgg
    17221 ttcataccat gatgtcgatt caaatgaaac tgccttccgt gttgctgctt ctatggcatt
    17281 acgtgcagca gctaagaaag ctaatccagt aatccttgaa ccaatcatgg ctgtagaagt
    17341 tgttatccct gaagattatt taggtgatgt tatgggtcac gtaactgctc gtcgtggacg
    17401 tgttgaaggt atggaagctc gcgctggcgg ccaacaagtt gttcgtgcga tggttccact
    17461 tgctgaaatg tttggttatg ctacaactct tcgttctgct actcaaggtc gtggtacatt
    17521 tacaatgaca tttgaccact acgaagatgt accaaaatca gtacaagaag aaatcattaa
    17581 gaaaaacggc ggaaaagctg aataatttct tgaaaccgga tagtcctgca aagagattat
    17641 ccaaagagac tatattttta atatagtttc gtgtaaaata attatcgatg atatgggcat
    17701 gtggtgtacc ttgtgtacat catataccta tcagacaaat aaaaaatata ttgtattttt
    17761 aggaggacat tttaaaatgg caaaagaaaa atttgaccgt tctaaaccac acgttaacat
    17821 tggtactatc ggacacgttg accatggtaa aactacatta acagctgcaa tcacaactgt
    17881 actatctaag aaaaacggcg gccaagctat ggcttacgat caaatcgatg gtgctccaga
    17941 agaacgcgaa cgcggaatca caatctcaac agctcacgtt gagtatgaaa cagatacacg
    18001 ccactacgct cacgttgact gcccaggaca cgcggattac gttaaaaaca tgatcactgg
    18061 tgctgctcaa atggacggag ctatcttggt agtttctgct gctgacggcc caatgcctca
    18121 aactcgtgaa cacatcctat tgtctcgtca agttggtgtt ccttacatcg ttgtattctt
    18181 gaacaaagta gacatggttg atgacgaaga attactagaa ttagttgaaa tggaagttcg
    18241 tgacctatta acagaatacg aattccctgg tgacgatgtt cctgtagttg ctggatcagc
    18301 tttgaaagct ctagaaggcg acgcttcata cgaagaaaaa attcttgaat taatggctgc
    18361 agttgacgaa tacatcccaa ctccagaacg tgacaacgac aaaccattca tgatgccagt
    18421 tgaagacgtg ttctcaatta ctggacgtgg tactgttgct acaggtcgtg ttgaacgtgg
    18481 acaagttcgc gttggtgacg aagttgaagt tgttggtatt gctgaagaaa cttcaaaaac
    18541 aacagttact ggtgttgaaa tgttccgtaa attgttagac tacgctgaag ctggagacaa
    18601 cattggtgct ttactacgtg gtgttgcacg tgaagacatc caacgtggac aagttttagc
    18661 taaaccaggt acaatcacac ctcatacaaa attctctgca gaagtatacg tgttgacaaa
    18721 agaagaaggt ggacgtcata ctccattctt cactaactac cgtccacaat tctacttccg
    18781 tacaactgac gtaacaggtg ttgttgaatt accagaagga actgaaatgg ttatgcctgg
    18841 cgacaacgta acaatggaag ttgaattaat ccacccaatc gctatcgaaa acggtactaa
    18901 attctcaatc cgtgaaggcg gacgtacagt tggtgccggt gtcgttacag aaatcaaagc
    18961 ttaattcact taagttttaa agtaaagcaa catttattcg gacgtaatcg ctcggaacga
    19021 aggtcttagg atctttgttc cgagcttttt tatttattgc aggatcagga aaaaagacag
    19081 gagtattata aatagaagaa acacgaaata aaagagtcag tattttctaa atgaaacttt
    19141 atgaaaacaa aatgtttttt acaagaaaaa gccttgcttt gtgagtttat atatattata
    19201 atgacaaagg tgcgtttact ttagtgaatt gcacagacgt aatcagaatg atttcgtcgg
    19261 ctaagaaacg agaggttgcg acacgcccgg acgctttgcc acgaacgagc gtgacggaaa
    19321 ttttcgtgga gctatgtcta cttttcaaaa taggcgaagg agggaacaag atggcaaaac
    19381 aaaaaattcg tatccgttta aaagcgtatg aacaccgtat tttagatcaa tcagcggata
    19441 aaatcgtgga aactgcaaaa agaactggag ctagcgtgtc tggtccaatt ccattaccaa
    19501 ctgaacgcag tctttacaca atcatccgtg cgactcataa atacaaagat tcacgcgaac
    19561 aattcgaaat gcgtacacac aaacgtctta tcgacattgt gaacccaaca ccaaaaactg
    19621 ttgatgcttt gatgaagcta gacttaccat ctggtgtaaa catcgaaatc aaattataaa
    19681 actaaaacaa aaataatgga ggtgtactca tgaccaaagg aatcttaggg aaaaaagtgg
    19741 gaatgacaca aatcttcact gaatctggtg aattaattcc agtaactgtt gtagaagcta
    19801 ctccaaacgt agtactacaa gtgaaaacaa tggaaacgga tggctacgaa gctattcaag
    19861 ttggttacca agatatgcgt gaagttttat caaacaaacc tgcgaaaggt catgttgcaa
    19921 aagcaaacac ggctcctaag cgcttcattc gtgaattcaa aaatgttgag ctaggagaat
    19981 atgaagtagg aaaagaaatt acagtagatg ttttccaagc aggagacatt attgatgtaa
    20041 caggtactac gaaaggtaaa ggattccaag gggttatcaa acgccacgga caaagccgcg
    20101 gaccaatggc tcacggttct cgttaccatc gtcgtcctgg gtcaatgggt ccagttgcac
    20161 ctaaccgtgt atttaaaaac aaacgtcttg ctggccgtat gggtggcaac cgcgtaacta
    20221 tccaaaactt ggaagttgta cgtgtcgatg cagaaagaaa tgttatcttg atcaaaggta
    20281 atgttcctgg agctaaaaaa tctttaatta ctatcaaatc agctgtgaaa gctaaataat
    20341 tgtaggaaga aaggaggaac taaggaatgc cgaatgtagc attattcaaa caagatggaa
    20401 gccaaaacgg tgaaatcaca cttaacgaag aaatcttcgg aattgaacct aatgaaagtg
    20461 ttgtttacga tgcaatcgta atgcaacgtg cgtcattaag acaaggaaca cacgcggtta
    20521 aaaaccgtag cgctgttcgc ggcggcggac gtaaaccatg gcgtcaaaaa ggaacaggtc
    20581 gtgcacgtca aggatctatc cgctcaccac aatggcgtgg aggtggcgta gtctttggac
    20641 caacaccacg ttcttacagc tacaaacttc ctaaaaaagt tcgtcgttta gcaatgaaat
    20701 ctgtattgtc agaaaaagtt gctgaaaaca acttggtagc aatcgaaggc ttgaacttcg
    20761 acgcaccaaa aacaaaagaa ttcaaacaag ttcttgctaa cttatctatc gactctaaag
    20821 tattggttgt tctagaaaaa ggtaacgact ttgctgcttt atcagcacgt aacttaccaa
    20881 acgtttctgt agtaacttct gataatgtga gtgtgctaga cgttgtatca aatacaaaag
    20941 tgttagcaac acaaactgct cttactcaaa ttgaggaggt gcttgcataa tgaacttact
    21001 agacgtaatc aaacgcccag tgatcactga aaaatcaatg cttgccatgg atgacaagaa
    21061 atacactttt gaagtggaca ctcgcgcaaa caaaacttta gtaaaacaag cggtagaagc
    21121 tgcttttgac gttaaagtca aaaacgtgaa catcgtgaac gtacgtgcga aattcaaacg
    21181 tatgggcaaa tatgcaggat atacaaaaaa acgtcgcaaa gcgatcgtta cattgactga
    21241 agattcaaaa gaaatccaat tattcgaagc tgctgaataa gcactaatag ataactcatt
    21301 aaattaggag ggaaaaacgt ggcgattaaa aagtacaaac ctacctcaaa tggtcgtcgt
    21361 aacatgacag gttctgattt tgctgaaatc acaacatcaa cacctgaaaa atcattgtta
    21421 caaccattga aaaaccgtgc tggacgtaac aacaacggcc gcatcactgt acgtcatcaa
    21481 ggtggcggac acaaacgcca atatcgcgtg atcgacttca aacgtaacaa agataacgtt
    21541 gttgcaactg ttcaaacgat cgaatatgat ccaaaccgtt cagctaacat cgcgttagta
    21601 cactatgaag atggagtgaa agcttacatc ttagcaccta aaggactaga agtgggtatg
    21661 aaacttgttt caggtccaga agcagatatc aaagtcggaa atgctcttcc attagaaaac
    21721 attccagttg gtactgttat ccacaacatt gaaatgaaac ctggtaaagg tggacaattg
    21781 atccgttctg ctggtacaag tgctcaagta cttggtaaag aaggcaaata cgtattgatc
    21841 cgcttgaact ctggtgaagt tcgtatgatc ttagctgcat gccgtgctac aatcggttct
    21901 gtaggtaacg aacaacacga attaatcaac atcggtaaag ctggacgctc tcgttggatg
    21961 cgtaaacgcc caactgtacg tggtagcgta atgaacccta atgatcaccc acacggtggt
    22021 ggtgaaggta aagctcctat cggacgtaaa gcaccagttt ctccttgggg tcaaccagct
    22081 cttggttaca aaactcgtaa caaaaaagct aaatcagaca aacttattgt tcgtcgtcgc
    22141 aaaaataaat aattactttg accatgtgtc gcacattttg agaggaggtt caccatgggt
    22201 cgtagtttaa agaaaggacc ttttgtcgat gagcatttaa tgaaaaaagt tgaagctcaa
    22261 gcaggggttg aaaagaaaaa agttatcaaa acttggtcac gccgttcaac agttttccca
    22321 agttttgttg gatataccat cgcagtttat gatggacgta aacatgtacc agtttacatc
    22381 caagaagaca tggtaggaca caaattaggt gaatttgcac ctacaagaac ttatcgtgga
    22441 catgctgctg acgataagaa aacgaaacgc taatttgagg ggaggataaa tccaaatggc
    22501 agaacaaatc acatcagcta aagcaactgc aaaaacagtt cgcacttcac ctcgaaaagc
    22561 ccgtttagta attgacctta tcagaggtaa aagtgtcgca gatgcaattt caatcttgaa
    22621 attcacacca aacaaatctg ctggaatcat tgaaaaagta ttgatgtcag cagttgcgaa
    22681 tgcagaaaat aactttgact tagatgttga aaacttggta gtaagcgaag cttttgttaa
    22741 cgaaggacct acaatgaaac gtttccgtcc tcgtgcaaaa ggatcagctt ctccaatcaa
    22801 caaacgtaca agtcatatca cggtagtcgt atcagaaaaa taaggaggga taatcagtgg
    22861 gtcaaaaagt acatccaatt ggaatgcgtg taggcatcat ccgcgactgg gatgctaaat
    22921 ggtatgctga aaaagagtat gctgagttct tacacgaaga tttaagaatc cgtaaattta
    22981 tttcaactaa acttgctgat gctgctgtat caacagttga aatcgaacgc gcaacaggtc
    23041 gcgtaaacat ttcaattcac acagctaaac caggtatggt tattggtaaa ggcggttctg
    23101 aagtcgaaag cctaagaaaa gaattaaaca aattaactgg taaaagaatc catatcaata
    23161 tcgtggaaat caaaaaacca gatctagatg caaaattagt aggtgaagga atcgcacgtc
    23221 aattagaaaa ccgtgttgct ttccgtcgcg ctcaaaaaca agccatccaa cgctcaatgc
    23281 gtgctggcgc tcaagggatc aaaactcaag tatctggtcg tctaaacgga gcagatatcg
    23341 ctcgttcaga aggatactca gaaggaactg ttcctcttca tactttacgt gcggacattg
    23401 attacgcatg ggaagaagca gatacaacat acggaaaact aggagttaaa gtgtggatct
    23461 atcgtggaga aattcttcca actaaaaaaa acactgagaa aggagggaaa taatcatgtt
    23521 agtacctaaa cgtgtaaaac accgtcgtga attccgcgga aaaatgcgcg gggaagctaa
    23581 aggcggaaaa gaagtagcat tcggtgaata cggtttgcaa gctgttgatt cacattggat
    23641 cacaaaccgc caaatcgaag ctgctcgtat cgcaatgact cgttacatga aacgtggtgg
    23701 gaaagtatgg attaaaattt tccctcacaa atcttatact gccaaagcaa ttggggtacg
    23761 tatgggttct ggtaaagggg cacctgaagg atgggttgca ccagtaaaac gtggtaaaat
    23821 catgtttgaa atcgcaggcg ttcctgaaga agtagctcgt gaagcgttac gtctagcttc
    23881 tcacaaatta ccaatgaaaa ctaagatcgt aaaacgtgag gaaatgggtg gtgaatcgaa
    23941 tgaaggttaa agaaatcaga gaattaacca ctgccgaaat gcttgatcaa gaaaaacaat
    24001 taaaagaaga attgtttaat cttagattcc aattagcaac aggtcaatta gaaaacactg
    24061 cacgtattaa agaagtacgt aaatcgattg cacgcatcaa aactgtgttg cgtgaacaag
    24121 ctaagtaatt atggaaggag gccattaagc gtatgactga aggaagaaat caacgcaaag
    24181 tttatcaagg tcgcgtggtt tcagacaaaa tggataaaac aattaccgtt gctgtcgaaa
    24241 caaagaaaaa ccaccctatc tatggcaaac gcatgaacta ttctaagaaa tacaaagtgc
    24301 atgatgaaaa caacacagca aaagttggcg acatcgtgag aattatggaa actcgtccat
    24361 tatcagctac aaaacgtttc cgtttgttgg aagtagtcga agaagcagtt attatctaat
    24421 aacacgagat tttcctatat agattgaatg caaaatctga aaggaggata cacaacgtga
    24481 tccaagcaga aagtcgttta aaagtcgctg ataactcagg cgcacgtgaa attttgacga
    24541 tcaaagtcct tggtggatct ggtcgtaaaa ctgctaacat cggtgatgtg atcgttgcta
    24601 cggttaaaca agcaacgcca ggtggggttg tcaaaaaagg tgacgtcgtt aaagccgtta
    24661 tcgttcgaac aaaatcagga gctcgtcgta cagacggttc atatattaaa tttgatgaaa
    24721 atgctgcagt gattattcgt gatgataaaa gccctcgtgg aacacgtatc tttgggccag
    24781 ttgcacgtga actacgtgaa aacaacttca tgaagatcgt ttcactagca ccagaagttt
    24841 tataatcttg agacacttat caaaggaggt gcgaaacagt tatgtttgtt aaaaaaggcg
    24901 ataaagttaa agttatcact ggaaaagaca aaaacaaaga aggcgttgta ctagcagcgt
    24961 tcccgaaaaa agataaagtt attgtcgaag gtgtaaatgt cgttaaaaaa caccaaaaac
    25021 cttctcaagc tgctcctcaa ggtggaattg ttgagatgga agcgccaatt catgtttcta
    25081 acgtgatggt ggttgactct actggtgtag ctggccgagt tggctacaaa gaagtagacg
    25141 gcaaaaaagt ccgtgtttct aaaaaaaccg gtgaagtttt agataaataa aagtttaagg
    25201 aaggagggtc ttactgaatg aaccgcctaa aagaaaaata tcttaaagaa gtaactccat
    25261 cattgatgga aaaatttaac tatagctctg ttatgcaaac acctaaagtt gaaaaaatcg
    25321 tcatcaacat gggtgttggt gatgcggtat caaacgctaa aaacttagat aaagcagttg
    25381 aagaattaac tttgatcact ggtcaaaaac caatgattac aaaagctaaa aaatcaatcg
    25441 ctggattccg tttacgtgaa ggaatgccaa ttggtgcaaa agttacattg cgcggagaaa
    25501 gaatgtacga attcttagac aaattggttt ctgtttcact accacgtgta cgtgacttcc
    25561 atggtgtaag taaaaaagcc tttgacggtc gtggaaacta tactttaggt attaaagaac
    25621 aattgatctt cccagaagta gattatgatt tagtagataa agtacgtggt atggacatcg
    25681 ttattgtaac aacagcaaac tctgatgaag aatctcgtga gttgttgaca caattaggca
    25741 tgccattcca aaaataatta aaggaggcga actacgtggc taaaaaatca atgattgcta
    25801 aaaacaaacg tcctgcaaaa ttttcaacac aagcttatac tcgttgcgaa cgttgcggac
    25861 gtccacattc agtttatcgt aagttccatc tttgccgtat ttgcttccgc gaacttgcct
    25921 ataaaggtca aattcccggc gtgaagaaag ctagctggta aaaagtaaat tcgcataaga
    25981 aggaggtaaa agatcaatgg tcatgacaga tccaattgca gattttctaa ctcgtatccg
    26041 taacgcaaac atggttaaac atgagtcttt agaagtacct gcatcaaaaa ttaaacgtga
    26101 catcgctgaa atcttgaaac gtgaaggttt cgttcgtgat gttgaataca tcgaagatga
    26161 caaacaaggc gtgatccgtg ttttcctaaa atttggtaaa aacggcgaac gtgttatcac
    26221 aaacttaaaa cgtatctcta aaccaggttt gcgtgcttat gtcaaagctg acgaagtacc
    26281 aaaagtattg aacggtttag gtatcgcaat catttcgact tctgaaggtg ttgtcactga
    26341 taaagaagct cgtgaaaaaa atatcggcgg cgaagttatc gcttacgtat ggtaatcaag
    26401 tagaatctac aaggaggtgt ctttaagtga gccgtatcgg aaataaagtc gttgttatcc
    26461 ctgaaggagt aacagttact caagacggaa acaacgttac agttaaagga ccaaaaggtg
    26521 aattgactcg tactttttca gcagatatca aaatgaatat cgaaggaaac gaagtaactt
    26581 tcactcgtcc aaacgacagt aaagaaatga aaacaattca tggaacaact cgtgcgaact
    26641 tcaacaacat ggttgtaggt gtcagcgaag gtttccaaaa agcgttagaa ctaatcgggg
    26701 ttgggtaccg tgcacaatta caaggatcaa aacttgtatt gaacgttggt tactcacatc
    26761 cagtagaaat cacaccacca gctggagtaa ctgttgaagt accagctaac acacaagtaa
    26821 ttgttaaagg tgcgaacaaa gaagaagttg gcgaattagc tgcaaacatt cgtggaactc
    26881 gtcctccaga accttataaa ggtaaaggta ttcgttatgt tggcgaatat gtacgccgta
    26941 aagaaggtaa aactggtaaa taatcgttta gaaaaattgc atagaagcca gcagcaggaa
    27001 aaaggcattt ggctgaagtt taccttcaaa aaaatacatt tttacttgtg ccgcagcttc
    27061 tatttttcga aacgtattta ctgcttgaaa tagtagtaca ataaaaataa agaggtgaca
    27121 attgtgatta caaaaccaga taagaacaaa acacgtcaaa agagacatcg tcgtgtacgt
    27181 aacaatatct ctggtactgc tgagcgccca cgcttgaacg tttttcgttc taacaaaaac
    27241 atctacgctc aaattattga tgacgtagcg ggtgtaacgc tagcaagtgc ctctaccttg
    27301 gataaagaaa tttcaggtgg aacaaaaaca gaagcagcac aagctgtcgg taaactagtt
    27361 gctgaacgtg cagctgaaaa aggcattaaa gtagtagttt ttgaccgtgg tggatacctt
    27421 taccatggcc gtgtgcaagc tttagctgaa gctgctcgcg aaaatggact agaattttag
    27481 aagaaggagg aataccattc atggtttata tcgatccaaa acatttggaa ttagaagacc
    27541 gcgttgttgc aattaaccgt gtaacaaaag ttgttaaagg tggacgtcgt ctacgttttg
    27601 ctgctttagt tgttgtcggt gacaaaaacg gacacgtagg atttggtaca ggtaaagctc
    27661 aagaagttcc tgaagcaatc cgtaaagcaa ttgaagatgc gaagaaaaac ttagttgaag
    27721 tacctatggt tggttcaaca atcccacacg aagtaatcgg tgcttttagt ggtggtagaa
    27781 tcctaatgaa acctgctgta gaaggttctg gggttgctgc tggtggacca gttcgtgccg
    27841 tattagaatt agctggggta gcagatatca cttctaaatc tttaggctca aatacaccaa
    27901 tcaacgttgt tcgcgcaaca gttgaaggtt tgaaacaatt gaaacgtgca gaagaagttg
    27961 cagaacttcg cggcaaatca gttgaagaat taatcggata aggaggacaa caataatggc
    28021 tgaattaaaa gttactttaa aacgcagtat tatcggacgt cctcaaaacc aacgcgatac
    28081 agttaaagcg ttaggtctta caaagattaa cagttcagtg gttaagcctt ctaatgaagc
    28141 aattaaaggc atgatcaata ctgtttctca cctagtggac gttgaagaaa tttaatttag
    28201 aaaaatcata actagaacat taaggaggtg ccaaacctat gaaacttcat gaattaaaac
    28261 ctgctgaagg ttcacgccaa gtacgtaacc gtgttggtcg tggtacttca tctggtaacg
    28321 gaaaaactgc cggacgcgga caaaaaggac aaaaagctcg ttcaggcggc ggtgtgcgtt
    28381 taggatttga agggggacaa acaccattgt tccgtcgttt accaaaacga ggattcacaa
    28441 acgtgaaccg taaagactat gcagtcatta atcttgatgt attgaaccgc tttgaagacg
    28501 gagctgaagt aactcctgtt gctttagttg aagctggaat cgtgaaaaac gaaaaagctg
    28561 gaatcaaagt attagctaat ggggaactaa acaagaaatt aactgtgaaa gcagcaaaat
    28621 tctctaaagc agctcaagaa gcaattgaag cggctggtgg atcaatcgag gtgatctaat
    28681 gtttagacta ttaaaagatg cttttaaagt aaaagacatt agatcgaaaa tcctatttac
    28741 tgtatttgtg ttgtttgtat ttcgcctagg tgcacatatt actgtaccag gcgtaaatgc
    28801 tgcttcattg caaaaccttt cttctttgcc atttttcaac atgttgaact tggtaagtgg
    28861 tagtgcgatg caaaactttt ccattttctc aatgggggtt tcaccataca ttactgcttc
    28921 tatcgtcatt cagcttcttc aaatggacat cgtgcctcga tttgttgaat ggtcgaaaca
    28981 aggggaagtt ggtcgtaaaa aactgaatca agcgactcga tatctgacat tagtattggc
    29041 atttatccaa tctgtcggta tcactgctgg attcaatcaa tacgctcaat taggtttcgt
    29101 gaataatcct aacgtagcaa cttacgtaac gatcggtttg attctaacag ccggtacgat
    29161 gtttgttact tggctaggag aacagatcac agaaaaaggt atcggaaatg gcgtatcaat
    29221 gatcattttt gcaggtatca tttctagatt gcctgaaagt atcagagaaa tttatgaaga
    29281 ttactttata aatattgatc cttcagacat atggaaatct gctatttttg tggccatttt
    29341 actagtagcg gtcttgttga tcgtgacatt cgtaacattt ttccaacaag ctgaacgcaa
    29401 gatcccgatc caatatacga aacgcgtagc tggcgcgcct acaagcagct atctgccgtt
    29461 gaaagtaaat gcagctggag ttattccagt tatctttgct agttcgttta ttgctacgcc
    29521 aaatgctgtt ttacaagcat tgagcggtag attcggttct gaaaactggt acaaagtgat
    29581 gatggagatc tttaactata atacagcacc tggtgctacg atttatacga ttctgatcgt
    29641 tgccttcact ttcttctacg cctttgttca agtaaaccct gagaaattag cggaaaactt
    29701 gcaaaagcaa ggaagttata taccaagcgt acgccctggt aaggggactg aagagtatgt
    29761 ctcaagatta ctgatgcgtt taagtacagt tggtgcagtc ttccttggtt tagtggcatt
    29821 gcttccaatc attgctcaaa tggtttggga tcttccacaa tcaatcggat taggcggtac
    29881 aagcttactg atcgttatcg gtgtagcttt agaaactgcg aaacagttag aaggcttaat
    29941 gctgaaacgt aaatatgttg gattcattga gtaagaaagc gtgttgaggt ttcccctcaa
    30001 cacacgttct cacacattta ttaggaggga aaacaatgaa cctcatttta atggggctgc
    30061 caggcgcagg taaaggaaca caagctgaaa aaatcattga tgcttatggt attccgcaca
    30121 tctcaacagg agatatgttc cgcgcagcga tgcaaaacga aacagctctt ggcctggaag
    30181 caaagtctta tatggataaa ggtgcattag ttcctgatga agtaacaaat ggaatcgtga
    30241 aagagcgact agctgaacca gatacagaga aaggcttctt attagatggc tttccacgta
    30301 cgctagatca agcaaaagct ctggacgcaa tgctaaaaga tttgaataaa aaaattgatg
    30361 ctgtcatcga tatccatgtt ggtgaagaaa tcttggtcga acgtctagcc ggccgattta
    30421 tctgcagcaa ttgtggagca acttaccata aaattttcaa ccctacaaaa gttgaagaca
    30481 catgtgatcg ttgcggtggg catgaattct atcaaagaga agatgataaa cctgagacgg
    30541 ttaaaaatcg tctggctatc aacatcaaaa atagtgaacc aatcttggct tattataaag
    30601 aacaaggttt gctaaacact attgatggtg ctcgtgaaat cgatgctgta tttgcagatg
    30661 tacaaaaaat cattgaaaaa tagcacgatt gcccgtagcc gacttgcatt atgcagttac
    30721 tcttgtcaag ttcaataaat tatgatagaa tatgtcagtc cgacttctag agaatcctct
    30781 agatgatcca ttttgagaat tttgaggtgg gaactgcagt ttccgccatt ttatcgtgtg
    30841 aaaatgcgaa acaagtacaa ggaggtactg tgcgtggcaa aagaagatat gattgaagtc
    30901 gaaggtacag tcgtcgaaac tttgccgaat gcaatgttta aagttgaact agaaaacgga
    30961 caccaagttc ttgctactgt ttcaggtaaa attcgtatgc attacatccg tatcttacct
    31021 ggtgacaaag taacggttga attgtctcca tacgatctta accgtggccg tattacttat
    31081 cgctttaaat aattgtactc cgtaaaacta caggaggtat tatcatgaaa gtaagaccat
    31141 cagtaaaacc aatgtgcgaa cattgtaaag taattcgtcg taaaggacgc gttatggtga
    31201 tttgcccagc aaatccaaaa cataaacaac gtcaaggata attggaggtg ctataaatat
    31261 ggctcgtatt gcaggagtag atattcctcg tgataaacgt gtagtagttt cacttactta
    31321 tatctatggt attggtaaca ctactgctaa aaagatctta gctgatgctg gtgtatctga
    31381 agatgttcgc gttcgtgaat taacgaatga acaaacagat gctatccgtg cagaagtaga
    31441 taaattaaaa gttgaagggg atcttcgtcg tgaagtgaac ttgaacatca aacgtttgat
    31501 ggaaatcggt tcttaccgtg gaatccgtca tcgccgtgga ttgcctgttc gtggacaaaa
    31561 cacgaaaaac aatgcacgta ctcgtaaagg cccagctcgc gctatcgctg gcaagaaaaa
    31621 ataattctta agtgaaggag gttaaaactt catggcagca aaaaaagtga atcgtaaacg
    31681 ccgtgtgaag aagaatatag aagcaggtat tgcacatatc cattctacat tcaataacac
    31741 tatcatcatg atcactgata ctcacggaaa tgcattagcg tggtcatcag ctggttcatt
    31801 aggtttcaaa ggaagcaaaa aatcaactcc ttttgccgct caaatggcag cagaaactgc
    31861 tacaaaagca gcaatggaac acggattaaa gactgttgaa gtaacagtta aaggacctgg
    31921 ttctggacgt gaagcagcaa ttcgttcatt acaagctaca ggtttagaag tgactgcaat
    31981 tcgtgacgtg actccagttc ctcataatgg tgcccgccct ccaaaacgcc gtcgtgttta
    32041 atgagtgccg ctcattttga gttttatcag taacgtcatt gaacaagaca cttttcgttt
    32101 tgaaaggggt aaagataaga atgattgagt tcgaaaaacc aagaatcgca aaaattgatg
    32161 aagaaaaaga ttatggcaag ttcatcgttg aacctcttga aagagggtat gggactactt
    32221 tagggaattc cctacgtcgt attttattat cttctttgcc aggtgctgcc atcactaata
    32281 ttcaaatcga tggtgtactg catgaattct ctaccatcaa aggtgttaga gaagacgtca
    32341 ctcaaatcat cttgaacatt aaaggcttag cgctgaaaat gtatggccaa gaagaaaaaa
    32401 cccttgaaat cgatattacc ggacctgcta cagtaactgc tggcgatatt atcgttgata
    32461 gtgatgtaga aattcttaac aaagatatgt acatctgtag tgtctctgaa ggagctacgt
    32521 tccatgctcg cttaacagtg aaacctggac gtggttatgt tcaagcagat gaaaataaaa
    32581 aagaagatat gccaattggc gttcttccag tcgattcaat ctacacacct gttcgtcgtg
    32641 tgaactatca agttgagaac acacgtgttg gacaccgtga agattttgat aaattgacga
    32701 tggagatttg gacagatggt tcaattgaac cgttggaagc aatgagtttg tctgcgaaaa
    32761 tcatgactga acatttggat atttttgtga acttaactga tgaagcaaaa aatgctgaaa
    32821 tcatggtcga aaaagaagaa acacaaaaag aaaaaatgtt ggaaatgacg atcgaagagt
    32881 tagacttgtc tgttcgctct tacaattgtt tgaaacgtgc aggaatcaat actgtacaag
    32941 aacttacaaa taaatctgag cctgaaatga tcaaagtccg taacttagga cgtaaatcat
    33001 tagaagaagt taaagcaaaa ttgcacgact taggtttagg tttacgtaaa gaagattaaa
    33061 accttttacg aaggagggaa accaacgtga gttatcgtaa actaggacgc acatctagcc
    33121 aacgtaaagc gatgttgcgt gatttaacaa ctgatttatt aatcaacgaa cgtattgtga
    33181 ctactgaagc tcgtgcgaaa gaagttcgct caactgcaga aaaaatgatc actttaggta
    33241 aacgcggaga tttgcatgca cgtcgtcaag ctgcggcttt cgtacgtaat gaagttgcca
    33301 gcgtgcgtga agaaaatgag gacatcgtca ttgaatcagc tttacaaaaa ctgttcaacg
    33361 atattgcacc tcgttacgca gaacgtcaag gcggatacac acgcattttg aagacagaac
    33421 caagacgcgg tgatggtgca ccaatggttg tcttagaact tgtctaaaat cacactaata
    33481 acatcacttt atagatgatt cttttagagt gttatgatgt tggaagatat ttttccgagt
    33541 ctagctcagc gctgctcctc aagcgaatat cgcttgggga gtaagcactc atcataaagt
    33601 gtttttttat accttcaatt acctgtcatg acagatatgt ttgaaggcat aaaaaaacat
    33661 tttaagcgag aaataattga aaattttgtc tttttttgat agtgttagta ggaactttag
    33721 gatgaagaag gagtaatcat ggagccaatt attgaattag aaaagataaa ttataaatac
    33781 cagccagatg atcttcgccc cgcattgaaa gatgtctcct ttacaattga taaaggtgag
    33841 tggatcgcta ttattggaca caatggttca ggaaaatcga cacttgccaa aacgatcaat
    33901 ggattgcttc tgccagaatc agggatcgtg aaagtgggaa atcaaatatt ggatgaagaa
    33961 aatatttgga cgatccgaca gatggttgga atggtttttc aaaatccaga caatcagttt
    34021 gttggttcga cagtagaaga tgacgttgct tttggtcttg aaaatcaagg gattcccaga
    34081 gaagaaatgc tagtgcgtgt gaaagatgca cttgagaaag ttagaatggc tgagtttgct
    34141 tcacgcgagc ctgctcgttt atcaggaggg caaaaacaac gtgttgccat tgccggcgtg
    34201 gttgccttac gacctgatat cattatttta gatgaagcaa ccagtatgct agatccagaa
    34261 ggtcgtgaag aagtgatctc tacgatcaag aagatcaaag aagaaagtca attgaccgtt
    34321 atatctatca ctcatgatat tgatgaagca gctaatgcca atcggatact agtgatgaga
    34381 caaggcgaac ttgtccgtga aggtacacca aaagaaattt tttctgctgg tcctgaactg
    34441 atcgacttag gtctagatct accatttcct gaaaaactga agagcgcact gaaagaacgt
    34501 ggggttgatg tgcccagtga atatatgaca gaagaaagga tggtggattg gttatggaca
    34561 tccgttttga acaagtagac ttcacttatc agccgaatac accatttgaa caaagagcct
    34621 tatttgacat caatatgacg atcaaagaaa acagttatac tgcattagta gggcataccg
    34681 gaagcgggaa atcaacctta cttcagcatt tgaatgcgct tgttaagcca acaagcggta
    34741 cagtccatat tggagaacga gatattcagc cggatacgga taataagaat ttaaagccca
    34801 tccgaaaaaa agttggcatc gtctttcagt ttccagaagc acagctattc gaagaaacgg
    34861 tagcaaaaga cattgctttc ggtcctaaaa actttggtgt cagcgaagaa gaagcactag
    34921 tcctagcaaa agaaacatta gaacaagttg ggctggatga aagctatttg gaacgttcgc
    34981 catttgaact ttcaggaggg caaatgcgta gagtagcaat cgctggtgtg cttgccatga
    35041 ggccggaagt acttgtattg gatgaaccaa cggcaggact tgatccccag ggcagaaaag
    35101 aaatgatgga gatgttttgg cgtcttcata aggaacagca gatcactatt gtattagtta
    35161 cgcatttaat ggatgatgta gccaattttg cagattatgt ctatgtgttg gaaaagggac
    35221 gaatcgttaa tagcggggag ccgcaagagg tctttcaaaa cattgaatgg ctaaaagaaa
    35281 aacaactagg cgtacctact gccaccgagt ttgcagaaga attaatggct aaagggatga
    35341 actttgctac cttgcctttg acagctgaag aattagcaga cgctatcgtc cattatgctg
    35401 gaggaaatgc ccatgatgaa taaattgata tttggtcgtt atatgcctgg agattccttg
    35461 atccatcgat tggatccccg agctaaatta ttagccagtt tttattttat cggaatcatt
    35521 tttttagcaa ataattggga aacgtacctt ttgttggcat ttttcacatt actttctgtc
    35581 ttcttgtcaa aagtagattt gaaatttttt atccgaggca ttcgcccatt gatctggcta
    35641 atcctcttca ctgtagcttt gcaggtcttg tttacaagtg gcggagaaac ttattggagt
    35701 tggggtgttt tccatatcac tgaatttggt gtgatgaacg gcctctttat tttctgtcga
    35761 ttcgtgttga tcatttttat gtcgactcta ctcacgctta ctacaccgcc tttggaatta
    35821 tcggatgcga ttgagtactt gcttcgtcca ttgaaagccg tacggttccc agtccacgaa
    35881 atttcattga tgctgtcgat tgctcttcgt tttgtgccta cattgatgga cgaaacagaa
    35941 aagatcatga acgctcagcg cgctcgcggt gttgattttg gtgaaggaaa cctagttcag
    36001 aaaatgaaag cagtcattcc tttgctgatt cctttatttg tcagcagctt caaccgtgcg
    36061 gaagatcttg cgactgctat ggaagcaaga ggctacaaag gtggggaagg aagaacgaaa
    36121 tatcgtgtat tgcattggca taatagagac acgatggtta tcattgcttt tgtattatta
    36181 actgtgctac tgatcttttt aagaggataa acgttcagtg ggccgctatc tattcttttc
    36241 ttgctgatct gtttgcaaga aaggatgcaa gcagtgtatg acaagaaata ttagggtctg
    36301 tggcagacta aggtcacagg cctctttctt ttatctgaaa agaaagaacg aaaaaatttt
    36361 atagaaaaat cattattcca gataaccttt taggaacaaa ggagagaaaa acatggtacg
    36421 ttacaaagca atcattgcgt acgacggaac aaattttaat ggattccaaa aacagccaaa
    36481 tgggcgtact gttcaagaag aagtagaaaa aacattacaa aaaatggcaa atggaaaaga
    36541 aatcacggtc tttggttcgg gaaggacaga tgccggagta catgcgatcg ggcaagtaat
    36601 ccattttgat tatccagagg agcgtccgtt agaacgaatg cgtttcgcgc tagataccca
    36661 gtcaccagag gatattgctg taagacaagt agaaattgtt tcagaagact tccacgcaag
    36721 atatttggtc aaagaaaaga cgtatcaatt tcgagtggat atagggaaac cacgcagtcc
    36781 ttttcgacgc cattatgcaa gttattttcc ttacccactt gatttggaaa aaattcagcg
    36841 ggcgcttcct gatttattag ggactcacga tttcacctct ttttgtgcat caggaagctc
    36901 gatagaggat aaaatacgta cgatctacga ggctaagatg gaggtaaatg aaacaaaaga
    36961 cgaattgttg tttacattta ggggaaatgg cttcttgtat aagatgatac ggatcttagt
    37021 aggaacgctt ttgaaaattg gcaatggccg attgcctgaa gatagtatcc cagatattat
    37081 tgcgaaaaaa gatcgtaatg cagcaggacc tacagcacac cctgaaggac tttatttgta
    37141 tgaagtgaaa tatgaataag aggttatgat agaagtggtc agcttcaaga aataagaagg
    37201 tatttacgaa aattacttct caaaattttg caaaatttcg gcttattttc gaagaagcta
    37261 cttctggaac accgtttaag gaagaggtcg tgaaatcagc gttttgacct gagtattaga
    37321 gaaacaaacg ggaaaatagc tcctcatatt ttgccgtttg ttgagctaat accgaaggtc
    37381 agctgattga acaccgttta agaaagaggt cgtgagcctg tcatttaact ctgagtcaca
    37441 aagagcaatt tcaaaaaaca gcttctcatc tttgatgaat atcgtgactt gtggacgagg
    37501 cgttgataag tgaatcatct ttttgttacc tattttttct gtaaccaaaa aacgatggaa
    37561 cgttgttttg attgcccttg gtgcggacag tctgaacagg ttccatcgtt tctttatttt
    37621 ctacgtcttc ttctgtgtgt gtttcgtggt tgttcttcta ttgctggatc aatcgtgcca
    37681 taaaaggggc gattgcggtc aaggtaccag acataaagaa taaacaatgc aatacatagg
    37741 acaaaaagga ttgcacctat cgtaaatcct tcaggcaagt aactgaaagt gacagtatgt
    37801 ttacctgctg gaacttttat catcaagaat gcgtctttga aattgctgac cttgactggt
    37861 ttcccatcaa ttttagcttt ccatcctttg tcataaggaa tagttgtcac caacatggaa
    37921 tcttcagcgg tatttacagt cccagaagct tttctgccag tggcttccat ttcgacacct
    37981 ttttctttga tctgattcat tgcggcttga taggctacaa catctagacc aacgacttta
    38041 ggctccatga aacttatttc tttcgtccca taaaaacttg cagtaaatgt cactgttgtc
    38101 tcttgaggat aatagccaag atcatagtat tgaccagaaa tgttgatttg cgtttttcgc
    38161 gatgtaccat tgacagtgat cgtcgcacta gaactttcta attgcgcaaa attggttggg
    38221 aacaaactta agtaagcttg tgtatttgca ggcacatgga cttcccaagt gagctctttt
    38281 ggctggttat ctgctgtttc tgagtaagtg acgccagtcg ttgtctttgt gatcgtcgta
    38341 ttcaactgat tcttcaaagt aggtgtatag aatgtaaaat actgttcatt tagaccagac
    38401 aatgtattga tcaagtttgt ctgactggtc aaattatctg tcactggctg ctcaacatca
    38461 ttaacggaca acggtgcagt aaatgctagt ggcaatgcat tgacattctg atacagtgta
    38521 tagtcgccgc tttgaccagc ttggcggaag ccatatttac tgaacgtgct ttcgttttta
    38581 gaaatgttat atttgattcc tgtaaaccca tccatcagca aagtattatt gttataacgg
    38641 atattcaaat ttgtccctct tgaacggaaa cctaatttat ccaggtaaga ggaagaatta
    38701 cgatttctta tagaagaaaa caaactgata ccactgtaac catagttgat cgaatcgttt
    38761 gcagatactg gatccagatt ttccaagcgg taaaacgagt cggaatcttt ttttgtttca
    38821 tttacaagag ctttcaagtc aggataagga tcagtatata aactgcggga agcataattc
    38881 caatcatcaa gaatgccatg gatcatactg ttcgtattga ccagtgcttc agcagacatg
    38941 atcaataata acaatatact cagatgattg aatgaaagtt ttttatgctg ataaaatccc
    39001 acagcaaaag cataaatccc taagaaaacc actgtcaata caaaggaagt catcgtcaca
    39061 taggtgtagc ttccttcagg tttgatgccc catgccaccg caaagagagc agctacgacc
    39121 aaaatgattc ctaacaacag cccacgatcg tcttgttcta tcttttccca tccttttcct
    39181 gcaagcagga gcactaagaa agagaacaga taggcataac ggaataaaaa catattcggt
    39241 gcatgcatac catgccagaa caaattcaat ggaacaagat aaaaacttgc gataagtatg
    39301 gcgaaaagac tgccgtacaa aaatttattt ttcaacggta ctttccgact ggcaaaataa
    39361 aacagacaga ggactaatgg tagcagacct gcgtaaacga acggaatcga tccgtatttt
    39421 gtcgtgtcat aaacaccaat catatttttc atgattagat ccagataaga agtcgcttct
    39481 gttttcaacg tagtgatctg actcagtgcc tcgccatttg accgaagatc cagcactgct
    39541 ggcaatacaa tgatcattga agcgccgcct gccagtaaag aagtgatacc ataaggaaca
    39601 atcgtttttt tatattctga ccagtttgtc aatagacggg cgataaaata taagaaagaa
    39661 aatacgccaa tcataaaacc catataaaaa ctggataaaa acaagagtaa ataactgata
    39721 aataaaagtt tcggccgttt aagatccata acacgatgaa tccccaaaat cactaatgga
    39781 agataaaaaa atgcatccag ccacatgatg atctccgaat gtgcagtgat aaatgagatc
    39841 aatgcgtaac tgacgctaag tgcaacatgc ccccagcgag gaatcttgaa cgtacgtgca
    39901 agaaaccaaa aagccaatcc ggcacagccg accttaagca aagtcagaac atacagtgca
    39961 tctggcatca tctggtttgg aaaaaagaac accagtggcg tgaagatccc gcctagatag
    40021 taagaaatta aagataaata attcaatcct agagaagcat tccatgtata aaaaatactt
    40081 tgctttccaa gaagaacatt tcggaagctg gcatgaaaat tagaaaactg tgaaaaggca
    40141 tcacttgcca aaatacttct gctgcttcca ggatagattc cgatacttag ataaacaagt
    40201 gctaaaatca aaaacggaat caggaaacta gctgctgcgt agcgtccgtt ttctctaaag
    40261 aatgacttta aatttttttt catatttatt ttccaccaaa tttctatgaa gttgtattca
    40321 gtttatcata tccttgtttt tctctgctta aaaagagtct agttttcaca aaaaaataaa
    40381 atttagaata aataacttca taatttgttc gtataacaaa aataaacgca aaaaaagggt
    40441 aggaat
//
LOCUS       DAHUVV010000016        39543 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.016, whole genome shotgun sequence.
ACCESSION   DAHUVV010000016 DAHUVV010000000
VERSION     DAHUVV010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.016"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..520)
                     /locus_tag="NK767_000851"
     CDS             complement(<1..520)
                     /locus_tag="NK767_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6693864.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVA"
     gene            731..1177
                     /gene="nrdI"
                     /locus_tag="NK767_000852"
     CDS             731..1177
                     /gene="nrdI"
                     /locus_tag="NK767_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCH6693865.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(1247..3229)
                     /locus_tag="NK767_000853"
     CDS             complement(1247..3229)
                     /locus_tag="NK767_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCH6693866.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(3243..4139)
                     /gene="murQ"
                     /locus_tag="NK767_000854"
     CDS             complement(3243..4139)
                     /gene="murQ"
                     /locus_tag="NK767_000854"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCH6693867.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(4142..5203)
                     /locus_tag="NK767_000855"
     CDS             complement(4142..5203)
                     /locus_tag="NK767_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCH6693868.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(5415..5981)
                     /locus_tag="NK767_000856"
     CDS             complement(5415..5981)
                     /locus_tag="NK767_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCH6693869.1"
                     /translation="MVKENFVNEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQALAEKVVDLPVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELISYE"
     gene            complement(6009..7511)
                     /gene="eat(A)"
                     /locus_tag="NK767_000857"
     CDS             complement(6009..7511)
                     /gene="eat(A)"
                     /locus_tag="NK767_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCH6693870.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(7769..8890)
                     /locus_tag="NK767_000858"
     CDS             complement(7769..8890)
                     /locus_tag="NK767_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCH6693871.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            9016..9957
                     /locus_tag="NK767_000859"
     CDS             9016..9957
                     /locus_tag="NK767_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCH6693872.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(10100..10996)
                     /locus_tag="NK767_000860"
     CDS             complement(10100..10996)
                     /locus_tag="NK767_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCH6693873.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            11278..12618
                     /locus_tag="NK767_000861"
     CDS             11278..12618
                     /locus_tag="NK767_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6693874.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            12813..13256
                     /locus_tag="NK767_000862"
     CDS             12813..13256
                     /locus_tag="NK767_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCH6693875.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(13518..14936)
                     /gene="nhaC"
                     /locus_tag="NK767_000863"
     CDS             complement(13518..14936)
                     /gene="nhaC"
                     /locus_tag="NK767_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCH6693876.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(15340..16563)
                     /locus_tag="NK767_000864"
     CDS             complement(15340..16563)
                     /locus_tag="NK767_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCH6693877.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(16595..17434)
                     /locus_tag="NK767_000865"
     CDS             complement(16595..17434)
                     /locus_tag="NK767_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCH6693878.1"
                     /translation="MKKNIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(17451..18131)
                     /locus_tag="NK767_000866"
     CDS             complement(17451..18131)
                     /locus_tag="NK767_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6693879.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(18133..19206)
                     /locus_tag="NK767_000867"
     CDS             complement(18133..19206)
                     /locus_tag="NK767_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6693880.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     misc_binding    complement(19265..19503)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(19546..19779)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(19856..20527)
                     /locus_tag="NK767_000868"
     CDS             complement(19856..20527)
                     /locus_tag="NK767_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCH6693881.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(20615..23293)
                     /gene="mgtA"
                     /locus_tag="NK767_000869"
     CDS             complement(20615..23293)
                     /gene="mgtA"
                     /locus_tag="NK767_000869"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCH6693882.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWLFITVVAYLALVTAVKKIYIRRFQELL"
     gene            23953..24390
                     /gene="ndk"
                     /locus_tag="NK767_000870"
     CDS             23953..24390
                     /gene="ndk"
                     /locus_tag="NK767_000870"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCH6693883.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(24412..25278)
                     /locus_tag="NK767_000871"
     CDS             complement(24412..25278)
                     /locus_tag="NK767_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6693884.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(25512..27590)
                     /locus_tag="NK767_000872"
     CDS             complement(25512..27590)
                     /locus_tag="NK767_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCH6693885.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            28083..29300
                     /locus_tag="NK767_000873"
     CDS             28083..29300
                     /locus_tag="NK767_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCH6693886.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(29759..30382)
                     /locus_tag="NK767_000874"
     CDS             complement(29759..30382)
                     /locus_tag="NK767_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693887.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(30538..31935)
                     /locus_tag="NK767_000875"
     CDS             complement(30538..31935)
                     /locus_tag="NK767_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCH6693888.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(31957..32928)
                     /locus_tag="NK767_000876"
     CDS             complement(31957..32928)
                     /locus_tag="NK767_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6693889.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(32935..35235)
                     /locus_tag="NK767_000877"
     CDS             complement(32935..35235)
                     /locus_tag="NK767_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HCH6693890.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(35237..36562)
                     /locus_tag="NK767_000878"
     CDS             complement(35237..36562)
                     /locus_tag="NK767_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6693891.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            36793..37218
                     /locus_tag="NK767_000879"
     CDS             36793..37218
                     /locus_tag="NK767_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693892.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(37716..38417)
                     /locus_tag="NK767_000880"
     CDS             complement(37716..38417)
                     /locus_tag="NK767_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCH6693893.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(38433..38873)
                     /locus_tag="NK767_000881"
     CDS             complement(38433..38873)
                     /locus_tag="NK767_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HCH6693894.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(38873..39448)
                     /locus_tag="NK767_000882"
     CDS             complement(38873..39448)
                     /locus_tag="NK767_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCH6693895.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT        11389 a         8425 c         6856 g        12873 t
ORIGIN      
        1 tagctactac aataaataaa aataatggca ctaatttaca aaatgtgacg atagcattga
       61 caaagctagc attttcaact ccacgattca ctagccatgc atatccccat aagaagatcg
      121 tactcatcac gatcgatgcc acattttgac caccttcgaa tacggggaag aaataaccta
      181 atgcactcat caacaatgtt gcgaacgcca cgtttcctaa ccagcatgtg agccagtaac
      241 cccaaccgct gataaaacca gcgaaacggc caaaaccttc ttctgcgtaa ctaaagatcc
      301 cgccttttaa atctggacgt tttttattga tattattgat cgataaggag agcatcaaca
      361 ttgctgttcc tacgagtacc cagctgaaga tagctgggcc accagccgat ccggagatgt
      421 ccgtcataat accaaatacg ccacctccaa tcgctgaacc aatcactaag gcagcaagtg
      481 gaatcaaacc aattcctttt ttctgttgct ctgtatccat gtaagcatcc tccaaagtaa
      541 aaccgctttt atcaaaaagt ataacaaacc ttttttattt ttacttaatt aataaaaact
      601 ttaatagaaa tataattcta ccgacttttt tctcattata caagcaaagc gcctagtccc
      661 tttccaaatt acaccaccaa aaaaacttaa aaagatgcta tactaaaggg aaagaattgg
      721 gggatgatcc atgaacattt tatatatatc gatttcagga aatacgcgtg cttttgccaa
      781 acatctagca gaatatgctg aaaaaatgca tgcagaagat cctgtcaatc ctgaagttac
      841 tttaaaagaa atccatgaaa atagtgattt tacgaaagaa acagaaccat tttttacttt
      901 cgttcctact tatttagacg gcgggaatgg gttagacaac ggtgacacag aaatcttgac
      961 cgaaacgatg cgcgaatatc tggaatacga agacaatcat aaactttgcc ttggtgtagt
     1021 cggtagcggt aataaaaact tcaacaatca atattgtctg acagccaaac aatacgcaca
     1081 aagattcggc tttcctttct tggcagatta tgaattacgg ggaacaccat ctgatgtgac
     1141 acgcgtttat caaattttgg cagaaaaaaa taaataacac aaaagaagaa gctgaaaaat
     1201 aaatttcggc ttcttctttt tatgttctct attgtttcta ttcttattaa gctttgacat
     1261 ccatcacagg tgcttttgct ttcgctttac ctgttttttc caagacaaag ttttttacac
     1321 gatccatatt ggttaccaag acgatcatgg aggtttcttt gtctgctgct ttgattgctt
     1381 ctaagtcagc ttctgctaca agcgtatcag gtgtcacttg ctgtccttct gttactttca
     1441 catcgaatgg cgtaccgcct aagtcaactg tgttgatgcc catgtgaagc aagatttcta
     1501 agccatttgg tgtcgtaatg ccgatagcat gttttgttgg gaaaacagaa cttattactc
     1561 cagtaaccgg ggaatagata tgtccgtttt ctggttctac aaagtaacct tcacccatca
     1621 ttttttgtgc aaaaactggg tcacttgctt tatctaaaga aacaactgtt ccgtcagcaa
     1681 ctgatgataa gatagtcaca ctttcagctg aagcttgagg tactgctttt tcaggtactt
     1741 catttactgt ggctgtttcg gtcgctagtt cttctttttc taaacggtct ttaggaatac
     1801 cgaagaagaa tgtagcaacg aagccaccag cgtaagcagc caataaacca aggatatagc
     1861 ctaaccattt accatcagag atcaatggaa tcaaagcggc tcctgaaggt ccaatagcga
     1921 ttgctccgac atttccgatc attccgatca ctgccccacc gatacctcca ccgatacaag
     1981 cagtaataaa tggacgtcct agaggtaatg ttactccgta aatcaatggt tcgccgattc
     2041 ctaagattcc gactggtaaa gcccctttga tcatttctgt taattctttg tcttttttgc
     2101 agcggatcca taatgccaat gctgcaccca cttgtcctgc tccggccatc gcaaggattg
     2161 gaagcaataa ggtacgacca gtttcatcaa tcatttgtac atggattggc gtcaagatct
     2221 gatgtagtcc gaacatgacc attggcaaga ataatgtacc tagaacgaag ccggcaaatg
     2281 ctcctccaac accaagtacc cagttgatcc caccgaccat gccgtcagaa atgaaccctg
     2341 ctaaaggcat gatgaagaaa atttcgatca gaccgatggc aatcagggaa agagttggcg
     2401 taacgatgat atctaccgca tcaggtacta ctttatgcag ttttttctca acgattgaca
     2461 ataaccaaac tgcgaagata acacctaaga tcccgccttg tccagttgca agagcttcac
     2521 cggtaaacaa gtttttcagt ggtgcttctg gattcatccc tgtcagcatc acaacagccc
     2581 cgataactcc accaagcgtt ggcgttgcac caaaaacttg tgcagcattg atacctgtat
     2641 agataactag atagctaaac atcccgtttt tcaagatgtt catcacatcg atatattgtt
     2701 gccatgttcc tgcgtctagt gttccagctg tgactaagtt tgctaaaact gcggcaatcc
     2761 ctgccacgat cccagtgccg acaaatgcag ggatcattgg tacaaagata ttagagatgt
     2821 cttttaatac cgatttgatt ttagagggtt tttgtttcga tttttgtgcc gctttcattt
     2881 cttgtgcccg ttcgtttaca cggtcttttc cagatgcaga agaacttgct gtagttgcac
     2941 tagcagaagg aagtttttct cctaatttca cgcctgctaa gtcaaccatt tcctgcgcta
     3001 ctttgttcac tttcccaggt ccgatgatga cttggagctg ttcatcctct actacgccaa
     3061 gtactcctgg aatcgctttt aattccgaga catttacaag atcatcattg atgaccttca
     3121 tacgtacacg agtcatgcag tggacaatgc tttcaacatt gcccataccg cctgctcctt
     3181 gataaatttg ttttgccagt tcttccaaag tcaattcgtt ttttgccatg gtcttttccc
     3241 cctcataagg ttccttttac aaaaccgttt gcttcagcta attttgcttg agctgcctct
     3301 ttatcactat ttgtcaaaat catgacgata gccagtttga cattttgatc tgcttcctca
     3361 aagtactttt cagctgtttc gtagtcagct tctgtcgctt gcatgatgat tcgttttgag
     3421 cgttcaatta gtttttcatt tgttggtttg acgtcgacca tcaagttatt gaatacttta
     3481 ccgataccga tcatactgat agtagaaatc atattcaaaa tcagtttttg tgctgttcct
     3541 gacttcaatc gagtagagcc tgtcaaaaat tctggtcctg catcgacttc gattgggaaa
     3601 tccgcatgct cgctgatttc tgcttgttta ttgcaagaaa tcgttcctgt aacagcacca
     3661 atcgattttg cgtaatctaa tccaccaatc acatatggtg tacgaccgct agctgcgata
     3721 ccaataacta tatcattttt tgttaaattc agcttgatta gatcttcttg ccctaattca
     3781 taagaatctt cggctccttc gacggctaca gtcattgctt cttctccacc agcaattagg
     3841 cctatgacca tttccggttc tacaccgaat gtcggtacac attcagcagc atctaataca
     3901 ccaagtctac cactcgttcc agctcccata taaatcaagc gaccaccttt tttaaaggat
     3961 tcaatcgtgt tggcgatcac tggctcgata cttggcagtt ctttttcaac agcagcggcc
     4021 actttctgat cttctttgtt catgagttgg actgcttgag aaatactcat ttcatctaat
     4081 ccaaaagttt cttggtttct gcgttccgtt gttaggttct ctaaattgat tttgctcatt
     4141 tttatcttcc ttcttttctt ataaattgat agttcatacc agctttgatt tggttgatca
     4201 gcggtagttc ttctttgatg actttggcta ctacattcac tttttcatcc gctggcaaat
     4261 catatttagt taattggatc tcacccatat agcgcaaata cttttcgtta tctaaagtaa
     4321 tagatccttt catacgctta gctgtatttc ttactgggat tggcggaatc tctcgaaaac
     4381 gcgcatctgc gcttcgtatg acatctcttg catcatcttg ccgattcgta tgttttccta
     4441 atatatattc gtaaaaaact tcatctacag gttccgtatg gagcaagact gtctgttctt
     4501 tttggaaaga agcaaattgc tcctgaacgt gttctgacag acctgcatct ccgatataaa
     4561 caatatccgt gttacttgct aatagatcaa gagcacctgc cagaggatgg atccctcgat
     4621 gttcttccaa agttggtaat ccttgataca gtggacctct caactctgta tctcctggta
     4681 caaagccttg tacaaggaat ccttgagttt tgagccattg atttttttcc tgataccatt
     4741 ctttgtccaa ccctgtttca agacgtggat aataattatg ccaagcttca atctgtgaga
     4801 aatcagccgc tgcttctttc aactcatcga tatcattagg cgtgatcgtg ctggcattca
     4861 aactgatttt caattgatgt gaccagtcgg cgatctgctg attagaaata tgataatcca
     4921 ttcttaaccc tgttacacca attgctttca agggtgttag atcttctaat gaaaagccag
     4981 ctcgtttcaa tgcctctcct gaaatatcga ccatcaactc tagtttattc tttttcgctt
     5041 cgcttcctag ttcgatcaat cgattttgat aagccgaaac atcatcttcc ggtatatgca
     5101 tggaagtgaa aataccgaca aagccattgg atgccatttg gcggatatac tgtttcttct
     5161 cttctgacaa agaatcattc ataaatattg aaaagccaaa catattatcc ctctttttct
     5221 gtatccgtct tcatttctat gactagagta tatatctaaa gaaacaaaat ttcaatactt
     5281 ttgttaaaat aattaaattt tatttctaaa aattggattt ataatgccaa aagaattgac
     5341 acagccacaa aaaaggcact gctaagcaaa agcagtgcct tgaaacattt tatttaaata
     5401 acacaaaagt ctaattactc ataagaaatt aattctgtat tgtctaccat gaatgtaaag
     5461 aacccttcat taggcattgg atgaaaataa gcattgatca ctcttatcaa ggcattatga
     5521 cagactaaca agtagacatt ttcttcgtct tccatgcatt ctttcaataa aggaacgatc
     5581 cgtgcgtaca catctaagac cgactctcca tttgggaaac gtacagcaaa cgctaaacgc
     5641 gctttttgaa agttttcatc tttgcttggc agtccgtcat aatccccaaa atccatttct
     5701 accaatcgct catcagcggt catcggcaaa ttgagacgat caactactgc ttgtgctgta
     5761 tctcttgctc gttgaagagg ggaatggatg attttcgtga ttggaacagg caaatctact
     5821 accttttctg ctagtgcctg tgcctgcatc tgtccttttt cagtcagcgg cacatctgct
     5881 cgcccgcaaa ctaatccttg tgcgttccaa gttgtttctc cgtgtctcgt tacataaagc
     5941 atgtgattct ctccttcgtt gacaaagttc tccttaacca taccttgttg tctttgagat
     6001 tacaaggttt agattttctc aagagaaata atctcagtag atactttatc tagaaaggtt
     6061 tgatcatgtt ccactagtaa catagcaggt ttcacgttca aaatcagttg ttctaattgt
     6121 tcttgattga agacatccaa ataattcaat ggttcatccc atatatagag ctcagctggt
     6181 tgggataaag atttagccaa ttccactttt ttccgttgtc ccatactcat ctgttcgatt
     6241 ttattatgaa aaacatctct ttccatccca agctttcgga gattattcaa aaatgcttga
     6301 tagtctactc ggtttttctt cgcgaattcc gccaatgtcc ctcgattgtc ttcataattt
     6361 tggcgtacgt aactaatggt caaatgtttg ggctgggatt tttctcctac aaccttgccg
     6421 ttgaatgtcc ccagaagata atggataatg gatgactttc ccgcgccgtt aggacctgaa
     6481 attgccatcc gctgatgagg ttcgatcgta aaatgaactg gctcgaataa cagattttca
     6541 taccctaatt gaagattttc tacgcttaac agccgtttat gatgagacac ttggctattc
     6601 atcgtcaaag catcgatata ctcgatatct tttaataatt tttctttctc actgatctgg
     6661 gtttccatcc gattcacgat tgctttagat cgtttcatcg tccgtgcagc atcagcgcct
     6721 actgctcctt tattcactcg cctagattcc gtatcgatga atccgacttt tttctttgtt
     6781 ttgtcccctt ctcgggaacg agaccattct gctttttcag ctgctgtttt ctttagcctg
     6841 ctgacttctt ttttcaattt ttcattttta gctatttcaa attcatcacg aagttttttc
     6901 tgttcttcat agatagagaa attcccttga taaagttcca gttggctctt ttcgattgct
     6961 aaaacatggt ccactacttc atcgataaat ccccgatcat gactcaccac gatgaatcct
     7021 tgttttttct ttttcaagta agccgctact tgttttctgc cagagatatc caaatgattc
     7081 gttggttcat cgattaacgg gaaatgagtg tcatccacaa ataagagtgc cagtaggact
     7141 tttgtcttct ctcctcctga tagtgtgctg aattctctcc ataagatttc aggatctgtt
     7201 tgcatcagtt ggagctctct ttcaatttcc catatctcaa aatccataat ctcatttaac
     7261 acataatagg tcaaacgctc tttatctttt gtttgctgcg ggaaatagac aaacgcttgc
     7321 tgatggatca cttgcccttg ataagggagt ttgttttgca ggatattcag taaagttgtc
     7381 tttcctcgac cgtttcgtcc gataagtcct agtttccatt gtgtgtcaaa gtttagattg
     7441 gcttgttcaa ataataatgt gccttggctg tcatagccga atgtcagatt ttttatttcg
     7501 attttagaca ttattccatc acctcgtaaa aaaagaccag tcctattttc agaactggct
     7561 aaagaggatg caagggttgg ggcacacaaa aagacccata catcgaaaga accaagccaa
     7621 tcccgaaaat ctgcacactg ctcccttgtc tccacaaggt acagtctgaa tgcagctttc
     7681 atcaatcgac ttaatttttc aatgcacgga tccctcactt tgtcctatat ttgaaattac
     7741 gatagcataa agaaattgtt tttgtcaact acttcgggag aatgttgaaa aatctgctga
     7801 gtgtctgtcc taagatttct gttttttcta atgtttgcct gtgttccatt gcttcctcta
     7861 aatcgattgg tcctaattga atgcttaaga caatggggag aaaatcaaag agtccttcca
     7921 gctctcggtc tctacttccg caaatagcga ttattggttt tcgatatttt tttgcaagca
     7981 ctgctacgcc atatggaact tttccgtgga ttgtttgctg atcaatccgc ccttctcctg
     8041 taatgatgac atccgcttgt tcgatctgtt tttctaatcc agtgatttgg ctgaccaact
     8101 caaaaccaga gaccatcttc gctcctaaca aaaaaagggc tccgccaata ccgcctgctg
     8161 ctccgcttcc tgggatgtca tctaaatcca cctcccaaac atcttttatc tgctgagcaa
     8221 tcttttcagc ttgtttatcc aagaaagcta acgtcgtatc cgttcctcct ttttgtcttc
     8281 caaattgcca agcagctcct tgttgacctg tatatgggtt ggtcacgtct gcagcgatga
     8341 tcacttttac tttagaaaca agaacgttcc ttcgatccaa atgaagtttt ctagtagata
     8401 acaaaggatt tccttctgtg tacccttcta aagaagcacc caaagattgg agtaatccta
     8461 gaccaccatc gctacatccg cttcctccta aagtgacaat gatttgttcc acttcttgaa
     8521 gcagtgtatc tttgataagc tctcccaagg cataactaga agcaagtcgg atggtttgat
     8581 ccgttggttt atcaagtaaa tctaatccta atacttgtgc agattcaatg actgcggtat
     8641 tttttccttg ccagttcgtc aataaataac tgatttttcg ctgacgaaat aagagatcca
     8701 tcccatcaat cgtacgccag gttcccccga gcgcgtaata gattgcttct gcagtatttt
     8761 ctccgccatc tgcaatcggc acattgattt ttcttccttt acggtcttcc caaccattta
     8821 gaactgcctg atttaattca aatgaagaag cacttccttt aaatgaatcg attgccgcca
     8881 aaatatccat ctatatccct cactttcttc ttaagtaaga cacttttttt cacttttttc
     8941 aaacaaaaca atgctatttt cttctgtttt tctatatact aaaagggaac tcattgaata
     9001 aaggagagaa aaaacatgaa aatcgcaata gctggagccg gagcaatggg cagccgaatc
     9061 ggattaatgt tgcatcaaag cggaaatgag gtcgtcttga tcgatcgctg gcctgctcac
     9121 attgaagcca ttcgtacaaa tggattgatt gctgacttta atggcaaaga agtagttgca
     9181 aaactgccaa tctattcacc agaagaaatc atcgaaagca acaaacacgt ggatttgatc
     9241 gtagccttga caaaagcgaa tcaattagat gatatgtttc gttcgatcca atccattatt
     9301 actgagaata cttatgtttt gtgtttactg aatggattag gtcatgaaga tgtactggaa
     9361 aaatatgtac caaagaaaaa tatcttattt gggatcacga tgtggacagc tggtctagct
     9421 ggtcccggaa aagtcacatt attaggtgat ggagaaatcg aactagagaa cttggaacca
     9481 gaaggcgaag catttacaaa aaaagttgta gaagtctttc aagaagcaaa attgaatcca
     9541 atctatagtc ataatgttcg ttattccatt tggcgaaaag cttgtgtaaa cggaacatta
     9601 aatggattat gcacgatatt ggattgtaat attgctgaat taggtgcgca aaaagcggca
     9661 gagggcatgg ttcgaacgat tgtatctgag tttgcttcta tcgcagcaaa agaaggaatc
     9721 acattagatc aagaagaagt gtaccaacat attgcttcta cttatgatcc tgacaacatc
     9781 gggttgcatt acccatctat gtaccaagac ctgatcaaaa accatcgttt gactgaaatc
     9841 gactacatca atggtgccgt ttggcgaaaa ggacaaaaat atgatattgc tacaccatat
     9901 tgtgcctttt tgacccaatt agtccatgca aaagaaggaa tccttggcgc agaataaaag
     9961 gctctctgat gagaatcagc tggatcagca gctggttctc atttttttac ccccatgaaa
    10021 gttcaaaaag aaaaacacaa ttctcatgtt gccataagaa ttgcgcttgt ttattttcat
    10081 ctatgattgg ttggcacttt tatttatttt taacagagga aagatcgtat tcatattgtt
    10141 cataaatagc ggagtcagta gtgaaaaaac gttttgccca attttctgca tcaaaaaatt
    10201 ccaacagttc tgatgggatt ggttgaaatg gcgtatgcaa aaattccggt aattcatcta
    10261 aatcacgttc ttgaagcaaa aaaatatttt cagaatgata aaactgataa tgagctatgg
    10321 cttgattatc tgtcactagt ttcttttgaa aatacataga ttcaaacgtt cgcaaagtca
    10381 ctccttgctg cccttcccgc aacagttcta gaatccccct cgattccccg gtttttttca
    10441 gatattcttc ataagtaagg tatccaggtt gttcatttcc tcgaagcgga agaataaagt
    10501 aattggtcgt aacacctctt tttgtaaatt cctgcttata tttttctgcc cgttcttttc
    10561 tgccgttatt tgttgcacca aaaaatagat ccgtctttgg ttcgctagaa gaaaaatcca
    10621 tatgtttcga ataaaaggaa ctattatgtt ttagtccaag ttctttcgct tctaaaaaat
    10681 caaaatgata aatctcatcg attgccggtt catcttgtgc ctgcttcagc atcgcctcgt
    10741 gttctgcttt aaagtggttc caaaaaaata acacgatttt tgcattggta aatggacgca
    10801 cgcgtcgagc catctccata tcaaatccat aatctaaaaa aacgatcagc tgataatttg
    10861 ccaactgttg tcgagctgat ttaatgaatt ccgactgtag taaatctttt ccggaccaaa
    10921 catctaaaga gcggatggaa ggaaaaaaat aaagtgattt atttttcgca attagcaata
    10981 cgtcgttttt gttcatctgt tccgcctcac tttctctgtc actttttcct atgatagcct
    11041 gtttaaatag taaaaagcca gtaaaacgct tcctattatt tttataaaaa tttaacattt
    11101 ctatgaaaat acgatgaaat aaatcattct tccattaaaa tatgcccaaa agttttctga
    11161 atagttgtaa ttatctgaaa atttgttatc attagtcatt attattatta ctatttattc
    11221 ttactattta ttcttactat gcattttgat tgtgaattta aaagaaagag gttatttatg
    11281 gaagataaaa aattgaaaaa acaacttagc tcccgacata tcaccatgct agctttaggt
    11341 ggagcgattg gtgcaggtct atttaaagga agtggcgaag cgattggaat cgccggccct
    11401 tctgtattga tcgctttttt gattggcggc ataattttat atatcgtgat gaaaggtctg
    11461 ggaaaaattg ttttaagtgg tggtgatacg caccacggat tatctggatt gattcgtcca
    11521 tatctaggtg ctcatgctgc cgattttacc gattgggtct actggtccat gtggattatc
    11581 aatatcattg ctgaagctgt tgcagctgct tcctttttac agttgtggtt cccccacatc
    11641 ccagcttggg tatttgtttt tatgttggct gttttaacta ctatcatcaa tctttattcg
    11701 gtacgattat tcgcagagac agaatattgg ctggcctttg ctaaaatcag tgtcattatc
    11761 ctattgatca tttttgctgc ttatcttgtc ggtcagcaaa tgttgggtac tggtgttttc
    11821 cctacccttc aacaattaac tgatcatggt ggttttactc cccacggtat gaagggaatc
    11881 atcagttctt tgcttgtagt tatctattct tacggcggtt ctgagttgat tgctatcaca
    11941 gtaagcgaag cagatgatcc taaacgtgcg attcctaaag caatcaaagg cgtcatggga
    12001 agaatcattt ccttttacat tattccttta tttttgcttt tgattatttt tccttggaat
    12061 actttagcag gcacgaacgt tagtcctttt gtgatggtat ttgaaaaaat gaatattcca
    12121 tttgcggcag atatcgtcaa tttcgtgatt gttcttgctc tgttctcgtc tatcaattca
    12181 ggtgtatatg cttcttcgag aattttgttc ttccgcttga aagatcggaa aggttcctct
    12241 agaaaactag ctgttttaaa caagcatcaa gtaccgcaac gtgctgtgct gttttgtacc
    12301 agtgttcttt atctaggtgt ggctctttct tattttgtcg gtgacaaatt atttggctat
    12361 ctggcaggtt ctctttccta tacagtatta ttgatctgga tcattatcag tgctgcgtcc
    12421 tttgtactga cactaaggaa aggaacgtta tgggacaaag gaatcagctt ttttgcatta
    12481 gcagtcttat ggttgatttt ccttggtatc ctattcacga actccattgg ggtaactgta
    12541 ttgaccggtt tgctttacct gttcatttat ttcagttatc aaaaaaagaa tgatgctttc
    12601 gtcttgacga atgaatgaaa ccaatagaga attttattct atttatattg aatatctgga
    12661 gctattgaaa aaaaggagct gcgacaaaaa ttgttgcagt tcttttttcc gaataaacgg
    12721 tgttcaaaaa ccatctgtgc agattaattg attgttctcc acgctcatga catgatagaa
    12781 gaaaggaatg aagaaaaagg agtttgtttt aaatgaaaaa gtattggaag tttttgttga
    12841 tgattggtgt ttcgacggta gtcatgtttg gtatgatgta tttgaatgtt tatgcgcttg
    12901 atcatatctt cttcagtcgc acacgtgtat tcatggcgtt gatgatgggt gcagtcatgg
    12961 cagtcatcat gttgttgttc atgtggaaaa tgtatgaaaa caaaaagata aatgctatga
    13021 ttttagccgc tagtcttctt gtttttgccg gttccttatt tatggtacga agccaaacaa
    13081 ctgtcaacga tacggcgtgg atgaaggcga tgatccctca tcactctatt gctatcttga
    13141 caagtaaacg agctgattta aaagatcctg aagtaaaaaa attagcggaa gaaatcattc
    13201 aggcacaaga agaagaaatc gacaaaatga aaaaaatgat caaagaaatg gaatgaccat
    13261 tatccatcca aataaaaaaa gaggatgttc cagaagtgat caacttcaag aaataagaag
    13321 aagctacttc tggaacaccg tgaatcagag gttgtgaaaa agctgccctg catcaagtaa
    13381 taagaacagc caaacaaaaa tagcttctca tatttttcgt ttggttaggc ttattaccgc
    13441 aaatgcaagc ttttgaacac cgtttattcg taaaagggag tgtgacaaaa gtcttgtcac
    13501 actccctttt tacgttttta ttgttctaat tgcgctgtct tgcttactgc ttcttgcggt
    13561 ttcacaaatt tctttgagat cccccaacct gtaaaggcac tgaccatcga aaacacaggt
    13621 gaaagcaaac tgaaaaacac gaatggcaag taagtgagtg taggaatgcc aaatgtactt
    13681 gctacgaaac ttccggcgat tccccaaggc actaagtagt tgattactgt tccaccatct
    13741 tctagtgttc tccctaatac agttggatca aatcctgctt tttcgtaagt ttctttaaat
    13801 gcattaccag gcaggataac agatagaaat tgttctccaa taaagatatt tactccgatg
    13861 cttgtcatca atgttgtaaa aatcaatttt ggggtgttcg tcatctttct tgatactcgt
    13921 tccataacgg ttgagatcaa cccaaattcg atcaacaaac cacctaatga caatgttaga
    13981 ataatcaatg acacagttgg catcatgctt tcgattcctc cacggctaag tagtgaatcg
    14041 atttgttggt ttccagtatt tgaaacaaat cccgattcga tgacccctgc caacttcgtt
    14101 aaggaagttt gtgggttttg aataaagatc attcctactg ctacagcaat attcaacaaa
    14161 atcgtcaaaa ttgccggtac ctttttccat gcacacaaga gcattaaaaa tagaggaatc
    14221 agtgaccaga tcgaaatagt aaagtttgct tctaaaatat cagtcacttc acgaatagat
    14281 gaaagacttg cggctttact tgtgtgtcct aaaatcatga ataaaaagaa agaaacaaca
    14341 aatgccggaa cagttgacca catcatatgc ttgatgtgtt tgaacaaatc ggcatcaaca
    14401 atagctgctg ctaaatttgt tgattcggat aatggagaca tcttgtctcc aaaaacggca
    14461 cctgaaacaa tggctccaac aacaagggct ggatttagtc ctagtgttgt cccaataccg
    14521 aaaaatgcaa tcccaatcgt tgacataaca gtaaaggcac tccctacagc gctacctacg
    14581 attgcacaaa caacaaatac agaagggacg aaccactgga cactaatcaa tttaaatcca
    14641 atgaccatga gggttggaat aattcctgct tgtatccata cagcaatcaa tgaaccgact
    14701 aaaataaaaa taaagattgg aatgatcccc ggtttgatgc cattgatgat cccttcgtgc
    14761 agccaatctg tcgggtattt ttttatcact gcgtacaaca tgaccattcc gatagctgcc
    14821 aaaatcgtga tattaggtga aacctttaat ccgacaacgc caatcgcaat gcctaaaaca
    14881 atgaccatca atacaagtgt tgcttctaaa aaacttggtg ttcctttttt ttccatgata
    14941 atctctcctt ttttagttgt tcttatgttc taaaaaagtt aagatatcgg attactccgc
    15001 cacaattcat ccgcgattcc tcctttataa aaataaaaag acctgctttc aactaaggac
    15061 gaatgactcc gcggtaccac cttaattaaa aacagatctg tttttcactt tcaaacgatt
    15121 attcaaatgt atggactttc catgtaattc gtcaattagg cttgatcggt tcacagcgac
    15181 caccgacttt ctgaacaaaa cacctaacca ctactgcgtg aaaagttgac ttgtatgaag
    15241 tttaccttcc ataagatcat ttgtcaatga aaaagaaaat attttttcat ttttctgaaa
    15301 attattttta taactttcgc gcacaacttt atctttttac tagaaagtag cgccctcttg
    15361 tttttttctt gctactattt cttgatgttc ttgtttgatt ttttctaata gcttgggttc
    15421 tctcaataaa tctagagctg tattggcaag taattccgct gcaatcgcaa tagaagcaag
    15481 tcctttttca cttttagcag cagctttgaa ttcttctgta tgtccggcaa tatatgtctc
    15541 agagattgct acagtaggct gtatcgttgg aataacttgg ctaacatttc ctacatcaga
    15601 agaacctaga cttgttcttt gttccgtttc tatttcttca tcaggaaccc ctgcttcttt
    15661 tgcatgttgg aagaataagt catcgaaaga aggcgttacg atgacatcat cgactgcatt
    15721 ttggaacaat ccaaactcaa aagtcgtacc cgtgcttaat gcagcacctc gtacgatatt
    15781 ttccactttt tcatatactt tgtctaatgt ttgtcgattt cctgcacgta aatagaaacg
    15841 tccagcagcg tagtctggta tgacattggc cgccacgcct ccatctgtaa tgattccatg
    15901 gatattgaca tcttttggca attgcaagcg tagagcgttg attccgttaa acacttggat
    15961 caaggcttcc aaagcattta ctcctttttc aggtgctgct gctgcatgag aagcgacacc
    16021 gtaaaatttg atatcgactg gatcatttgc taaagattct gtcgttttcc cataacgaaa
    16081 agctggatgg acacataatg ctgcatcgac atcctcaaaa aaaccttccc gaacgaagct
    16141 tcccttagct gatccatttt cgcctccttc ttcccccgga gtaccataca cacgaatttc
    16201 tccgccaatc tcatcaatga attgtttcac tatgctggct gctaatacgg agtaagtacc
    16261 aaataaatta tgtccgcatg catgaccaat acctggtaaa gcatcaaatt ctgccaaaaa
    16321 ggcaattgtc ggacctggtt tattggaaat ataacgtgca tcaaagcctg ttcggtgccc
    16381 agctacttct ttttctaccc agaacccttc tttttccaat tgtttgatta aagctgctga
    16441 ggaataaaat tcataattac ttacttcagg atggtcgtgg atagctagtg caagtgcttg
    16501 atattctggt aaacggtatt gaatcagatc cttcaattga tttgttgaga gtgtggttgt
    16561 cataaaaatt tcctcctatg ctagtcgttt tttcttattt tctcccgaat gtttcccacg
    16621 caggtacgct tgaacctttt gatgtttttt cgattacttt ggctgtttct tctgtttgat
    16681 aggcatctac tattttttga tacgttttat tgtcttcatc ttcttttctt gcaacgatga
    16741 tattgacata aggacgtgca tgctcatcga ctggttctaa gaagattgca tcagaagcgg
    16801 gattaaatcc agcatctaca gctacaccgc tattgatgac tgaaatatct acatctgaca
    16861 aagcacgtgc tgtttgggaa gcatctaact cagaaatgtc tagatttttt ttattctcgg
    16921 taatatcaga aacggtaggc gcttggcctt ttgccttgtc cactttgatc aatccagcag
    16981 tttgcaataa cagaagagct ctgccgccat tcgtgacatc attagggatt gcaacttttg
    17041 ctccatcttt gatatcagcc acatctttta acgtattgga ataaattccc aacggtgcat
    17101 taaccgtatt cccaatcgat actaaatcag ttccgtgttc ttcattgtaa ttgtctaaaa
    17161 agaattgatg ttggaaggaa ttcaaatcga tcgatccatc tgctaaagcc gcatttggct
    17221 gattgtaatc tgtaaatttc acatattcca aatggatgtt ttcctttttc aaacggtctt
    17281 tcacatcgtc ccatacatct gtatcttccc caatgatccc tagttttacg gtcgtttctt
    17341 ttttttcagt tgtagctgct tttccttggc tgcatcctgc caaccaaata attgctacaa
    17401 atgtaatgac tgcaaaacga atattttttt tcattttttc tcctccattt tcaatgtgtg
    17461 acttttttaa tcagttgatt actgataaat tgactcaaaa aaactaagac cagaatcaat
    17521 aatgtagcga caatcgttac atctgtttgg aatcgattat atcctctcga tatagcaaga
    17581 ttccctaatc ctccggcacc tactgctcca gccatggtag tcagtccgat cacattgatg
    17641 atcgtcaacg ctgagacccg aatgatacca ggtaaccctt ctaacaaata aacacgtaaa
    17701 atgatgccga ttgggctcgt gcccatcgct tctgctgctt cgatcacccc aggatcaact
    17761 tctagcagtg cattctcgat ttgacgggca aaaaacggga caacgccaaa aatcaaaggg
    17821 actaaagctg ctttggcacc aatcgtcgtt ccaactaaga aacgagtagt caaagctaaa
    17881 agagaaagca agataatgaa agggatggaa cgaatgatat tgatcaactt atcaagtatt
    17941 tcgaacagca gcctgttagc tagtaatcca tttggacgtg tcactactaa cgtcacacct
    18001 aagatgattc cgatgatccc accaatgaat gccgtccaaa aagacatata tagtgtttgc
    18061 tttgtcgctt cgatgaattc atccggaatc aaatagacat tcggcaaata tgttttaaaa
    18121 aattcagtca ttttagtttc ctccttcgat gactttgata cggttatcta attcattttt
    18181 ttgtaaaata gttactttta caccagattt aatcaaataa tccaatgctt tttgccgctg
    18241 tttggtttct ccagctaaag taacgatcaa acttcctaag ggtgtttctt gtaatagctc
    18301 tacattaccg tataagatat tcgctgttac ttggtacttg ctgtaaagat gagcgattag
    18361 aggttcgttc gtctggttgc cgatataact taattccacc aaccatttat ttgcaatctg
    18421 atcggcaaaa gcagcatggg cactgattgt ttccaatgct tggtcaaggt gtgtcgctgt
    18481 ccgaataaaa tctttagtca attcttcttc aggatgactg aaaatctgga tgctgctacc
    18541 tttttcaatg attttccccg cttccataac cgctaccttg ttgcagattt ctttgacaac
    18601 ttgcatctca tgagtgatca ggacgattgt tagacctagc tgacgattta gtttttttaa
    18661 cagtgcaagg atctgaaggg tcgttttcgg atcaagtgca ctagttgctt catcgcataa
    18721 caacacttta ggttcatttg ccagagctcg tgcgattgcc actcgttgtt tttgcccccc
    18781 cgacagttgt ttaggatacg cagatgcttt ttcttccaga ccgacaagtt ccagtagttc
    18841 attcactttt tgacgacgtt cctgttttga ttttccagaa tactttagag aaaaatctac
    18901 gttgtcaaaa atcgttcgac tttccattag attgaaatgc tgaaagatca taccgatggc
    18961 ctttctttct tttcgcaatt cttttggaga gagctggctt aaattcgtct gattgacgat
    19021 gacttctcct gctgttggtt tctgcaacaa attcatcacg cggactaacg tactctttcc
    19081 tgcacctgag taaccgacga tcccataaac atcccctttt tcaatcgtta aatctacaga
    19141 atctactgcc tgtatctctt tttctttttg tttgaaacat acagaaatat ctcgcaattc
    19201 aatcattaac attcctcctt gtctataaaa aaagcatcct caagaaaatg aaaatagtca
    19261 ttttcttgag gacgcctttt tgcgtgttac cacctcatgt tcaagctgtg ttcacacaca
    19321 actcctcttc aagtaccatc atactctagc aagatatcgg tcgcagccgt caccgcctta
    19381 catctatgct cgggatgaat tgctcgatgg ccattttcca atagtcgttc cttgcttctt
    19441 ctcagctacc gaagctctct gtcaaaggtt cttctatcgt acttaccatt tcaatgcaaa
    19501 taatattcaa aaaaaaaatc cttttactaa cataaactat gtcagtaaaa ggacgacgta
    19561 tcgtggtacc accttttttc ggaagtttcc ttccctcaag cccaacatct caaaaagaga
    19621 gattggcgtt cgttttaacg gtcgaacatt tccgaatttg cctacttatc gacaaattat
    19681 tctccaaggc cattttcagc ttttcttact tgactcttca caccagccag agcctctctg
    19741 caaagtttcc aagcttactt tccttctcaa cgaaatcaat attttgttgt tgggaataat
    19801 ctacaggagg aaaactgaaa agtcaagcat gaaatccaaa ttattttttc tatatttatt
    19861 tttctgtttt tttatctaac atttcaataa cagttcgaag atcttttcct tccgcaagaa
    19921 ggttcaattt ttccttctct tcgacgctat aatttcgaac aaaactggaa tcgggaatca
    19981 aaacaacgcc tagtcctgca cgtgaagcgg ctgtcgctcc tactaaagaa tcttccactg
    20041 caaaagctga aagagcttct aatcctgttc gttccaaagc tgtcagataa gggactggtg
    20101 aaggcttgtt ctccgatact tcatctccga acgtaaacgt atcaaagtaa tcggctaatt
    20161 gaaagtgatc caagatatct gttgctcttt tcgtaacagt tgtcgtagca atccctagtt
    20221 tcatatgacg ttcttttaag tctgtcaata tttcttttgc atagggtttc aattggatag
    20281 ctccacttgt cagctgttga taaaaatatt cttcacgctt tgctcggact tcctgcactt
    20341 tttcaggact gcctgctgcc tgcactaaaa tatcaaaact ttgtttccag ttttttccgc
    20401 cccaagcgtc aacgatttct ttcgggatat gaatatcaaa ttgctggaat ccccaaagcc
    20461 aaccatcccg atacgttctt tccgtatcca ctaacaaacc atctaaatca aatattgcac
    20521 ctttcatcta tacattaatc ctttccaaaa caatacacct agtttagcat gactgatcca
    20581 aaatgaacag aagcatagag cccagccgca tcacttataa gagttcttgg aatcgacgga
    20641 tatagatttt cttcacagct gtcacaagcg ccagataagc gacgactgtt ataaacaacc
    20701 ataaccagta attgccaggg agcggcatca agcctaactc agcaccaaat cctgtgaatg
    20761 gaaggactga accgacaatg attcctaatg tagtgataac cagcatggca aatgttgcat
    20821 tgctttggat aaatggtatt ttcggtgtac gcaatgcatg gagtactaat gtttgtgtcc
    20881 acagggattc gacaaaccat cctgcatgga ataatgcgat aaaaatcact tgttgtgagc
    20941 tatccaatgt atggaaactt ccaccaacta cagcaggaca aataacaaaa tacatcaaca
    21001 agtaagtagt gatatcaaaa atcgaacttg ttggaccaaa ataaaccatg aattttccaa
    21061 tactagatgc attccatttc tttggctcat ccaaatattc tttatccatc cgatcccatg
    21121 gtaaggaaat gcaagaaacg tcatagatca gattcaaaaa tagaagttgc aaaggcaaca
    21181 ttggtaaaaa tggtaagaac gtactggcaa tcagtacgga aaacatattg ccaaaattag
    21241 aacttgctgt tgccttgata tatttcatga tgtttccaaa agtttctctt ccagaaagaa
    21301 ggcctctttc caaaatcgtc aaatcttttt ctaaaaggat cacgtctgct gattcttttg
    21361 cgatatccac agctgtatcc acggaaatac cgatatcagc cactttcatt gctggagcat
    21421 cattgatacc atctcctaaa aatccgacag tatgcccatt tttacgtaaa gctgtcgtca
    21481 atcgtgcttt ttgctgagga ttcaatttta cgaatacttc gtgttcttca gctacttgcg
    21541 ctaattcatg atcattcatt tccgagattt tctcaccagt aatcaattca ttgatttcta
    21601 ggcctacttg acggcacaca gaccgtgtga ctaattcatt gtccccagtc agaactttta
    21661 cagctacacc atgttctttt aaagctttca acgcttgttt tgttgtttct tttggtggat
    21721 caaggaaagc aagataacca atcagaacca tgtctgactc atctttgact gaaaattctc
    21781 caacaacgct tggattggtc ttttgtgcga caccaatcac tcgtaagccg tcttcattaa
    21841 gtgattgtac ttttgcaagc accgcttctt ttcgttcttt tgttaatggt gcgacagtac
    21901 caccaagatc aataaattta gaaatgttca acatttcttc aatcgcgcct tttgtgatca
    21961 tctgtgtttt gccagccgga tcagcgacta caacactcat acggcgacgt tcaaaatcaa
    22021 aaggaatttc atctactttt tgataattga ttgtatctgt atctaaagtt tctgctgctt
    22081 catcaatgat agccacatct aacaaatttt tcagccctgt ttgatagtag ctgtttagat
    22141 aagcatggcg caaaacacga tcatcttcat ttccttcaca atccatatga tattcaagaa
    22201 tgattttgtc ttgtgtcaat gttcccgttt tatccgtaca taacacatcg atcgcaccaa
    22261 aattctggat agcatttaaa tttttgatga ctgttccttt tttggccatc acacttgctc
    22321 ctttgaccaa attcgtcgta acgatcattg gaagcatttc aggcgttaaa ccaactgcca
    22381 cagataatcc aaaaagaaaa gcttctaacc aatctccttt tgtaagccca ttgataacga
    22441 taactgctgg agccatcaat gacataaatt tgatcagcag catggatgtt ttgtggatgc
    22501 ctaattcaaa acttgattgg atgggttttt cagatagcgt ttctgctaca tgcccaaata
    22561 gtgtgtcatt tccgacagaa acaatgattc cttccgcgct tccactgatg acattgcttc
    22621 ccataaagac aagattttca taacttgttt cactttgctg atcgtcttca catactttcg
    22681 catgcttttc tactggatag ctttcccctg tcaatgctgc ttgagaaatg aatagatcct
    22741 ttgcggacaa taaacgcata tccgctggta tcatatctcc tgctgaaagc cgaacaagat
    22801 ctccacagac gatttcttcc ataggaatct ctgtatactc gccatttcgt ttgacagctg
    22861 ccgttacttt cacaagtgat ttcagttttt cagctgcttg attagaacgc acagactgga
    22921 tcaacgtcat cgtcccactg acgataacca tagtgaagat aatggctaca ccaaagagat
    22981 ctttatctcc tggagcggct aatacgtatt ccgtaatgaa tgaaataatt cctaatgcaa
    23041 tcaacactaa tgtaaagggt gtgatgtatg ctttcaatat ttctataatg aatggtgttt
    23101 ttttgccata tgaaatcgca ttttcaccat aagtttctct tgaacgctcg atttgttctt
    23161 ctgacaatcc atttggtgat gcatggaaca attcaaataa ttcttgcttc gtttttccag
    23221 caaaagctgc atagattttt tgtgtatctt tctttttttc catctcgatc tctcctttgt
    23281 gtccaaaaga cagctgttct cgctgttttt ggtcttttgc tattcatcgg atcgatagag
    23341 aaaatgctga aaggggctga gaacagatag ataatagccc tggcctttcc tctaaatcct
    23401 tgtttttcga atgactgtca tccatttgtt ctcatctcct ttactaagta attttaattc
    23461 atgacttcca atcatttttc gaatagttat tttcccaact cgctcacctc cctttttgtt
    23521 ttttcaccta tgggatttgc ggcaacaaaa aaacctctcg ccaaatactc agcgaaaggt
    23581 ttgaaaaagc aaatcaaaaa aagcttctca gatctcatcg ttgagttttg acactgtaca
    23641 acgtaaaaag cagatatctg cttttagcta ccttatgaaa agccttatct cgacaattcc
    23701 tgttctaccc attggcgtct ctcgacgttt ttgggcagta gcctatgttt gtataggagc
    23761 ctcacctaac aaatcctatt cggttttccc tcgttaatat aacaactaaa aaagaggatg
    23821 tcaaatcaat tttccatgtt tttcatactt ttttaaaata cacgcgttat atttcctgtc
    23881 tcacgcagtt ttatttgtct cattttccac ctcccgctat aatagaatta tagtaggaaa
    23941 cgaggtgctg ttatggaacg aacacttgtt attatcaagc cagacggcgt aagacgccat
    24001 ttggtcggaa gcatcatcca acgttttgaa gcaaagggac tggccattgc tgaaatgaaa
    24061 tttgcaacga tgacacctga actagcaaaa gaacattatc agcacttgac tgaacgtagt
    24121 ttttttgatg agcttatcga ttacatgact tctggtccgg tcgtttatct tgtgctggtc
    24181 ggcgaggaag tgatcgatat cgtccgtaag atggtcggag caacaaaagg ggcggatgcc
    24241 gttccaggta cgatccgagg agattatgca ctgccgggga ctgaaaacat catccatgct
    24301 tctgattccc gtgatgctgc aacaaaagag atcgcccgct ttttcccgcc attcgataca
    24361 aacaaaccgt ctgaaaaagc ggcgaagtga tacaaatcct aaactgttgg tctaactgct
    24421 gggtttagca atggccagca gttttttctc taaagaattc ctttgattct tccctaatgt
    24481 atattcgatc actgcgaaaa actcttggtg attttccgat ttttcttccc ctaaagcagc
    24541 agaaatcagt cgttggtaat aatccgccat caatgtttca taaatcactg tacttctttc
    24601 tttatgcgct aaactccgtt tcatatcttc attcacgcta cgtgcctttt ttaagcgacc
    24661 attttctata taaaacaaag tcaagttcgt caaaaaagta aagagatact gctcgtgatt
    24721 attcagttgc gcgatccgtc gcattctttt tttgattttc aaataattat tctcaataaa
    24781 acgatcactg aatatccaag gaaaatctgc tagaatagat acttcaaatt gcccatactc
    24841 tggtacttgc atcaaatatt cttcaatgat acgacattca taacgcaaaa attcaaattc
    24901 cgtctcagat acttctgttt ttttggacat gtgcagattc aaaaccaaat agtacagatc
    24961 ttctgttttc ttatagtgcc tgcggttatt agcaatttct ttgtaaggat caaagctgtc
    25021 ttcttgaatc cgatctagca ttcgtctttt tttctttaat ttttttggct catcaagctg
    25081 atttcgtatc cattcatatt cggcaagtgt catgttcatt cgctctaaaa atgatttcaa
    25141 tgttccaaac gatacttttt tcactgaatt tgtctcgatc ccgactagcg aagttttaga
    25201 acagattcct tccgctaatt gggtctgtgt cagctttttg ttgactcgaa gcttcttaat
    25261 caattctcca tcatacataa tagttctcct tttttaattg aaattaaccc ttggcttttc
    25321 cagaatgctt gccaataaga ggaatgatta tttcaattta aattttccgc attcaataaa
    25381 tagatacgtt aaaaataaaa aatacacgaa aaaaataaaa aaatgttttt ctcataaaaa
    25441 ataccaacct tcaagcatga gactgctgtc taatccctga aggttggttt gattgtttat
    25501 tgatcatttt tttactgctc cgcattggtt cctgttactg gatataggat agtcgttgtt
    25561 ttattgccgt tttttgaaaa gacactggtc gaatgttcac ctttttgctt ttcgattgct
    25621 tcattacgtt ccgcttcgtt tttatagtca tattttttcg gatcaatcgt ctcaaatccg
    25681 tttggcacat aaaaccgcaa gagattttcc tgattcagct tatctgaaag acttaacgca
    25741 gtcttgactt tttcctgatc tttggagatt tcctcgtcga ttcctgctgc tttcgtatcg
    25801 atgatttctc ctgtctgatt attgtaagct tttcctccta ggacggtgta tttgggcgtc
    25861 acgaagttcc catttcgaaa agcaactact tgatcgtgct gagaagataa taagtctgta
    25921 ccaaaatgaa gataatcttt atcatcgatt cccaatagat gtagtaatgt cggcaaaaca
    25981 tcgatttctc caccatattc atgattgact gtccctttag tgtaaccagg catatggatc
    26041 atcaatggca ctcgttgcat ctgtgcgttg tcaaattcat cccatgtatc tggatcttta
    26101 ccaagagcac ttgctaaatc tttattatct gtattcgaga tcccaaaatg atccccgtaa
    26161 atcacaaaga ttgaattttg gtaaatacca gattttttca aatacgtaaa aaattgttcc
    26221 aatgattgat ccagataatg ggccgttcga acataattat ctactgtgct attgccagta
    26281 ttcaaagatg gaaagtcgaa attcttgtca tccgtataat aaggcgtgtg gttggttacg
    26341 gaaagaaatt tcgtgtaaaa cggctgttgt agatgttcaa gatattgggc agattctctg
    26401 aataaatctt tatctaatat cccataacct aacgtttcat cggattcatc aaaataagat
    26461 cgatcaaaga aattgtcata ccctaaattt ttatatacat gatctctatt ccagaaacta
    26521 gctacattcc catggaaaac agcacttgta tagccttctg tttgaccaag ggtcgctggt
    26581 gctgcttgaa agacattatc tgacccaagt tctgtgaata atgagccttg aggtaaacca
    26641 aatgtgccag tttccaacat gttttcagca tcacttgtct ttccctgtcc tacttcatga
    26701 aagaaattgt caaaactgat cgttgcttga ttctggaaaa tactatttaa aaacggcgtg
    26761 acttcttgtc cgtctacctt catattgatc aaaaactgtt gaaaactttc taaatgaagg
    26821 acaatcacat tttttccttt agcaatccca aatgtttcag gatttggctc tgcataatgt
    26881 ttctttgtat agtctaaaac ttcatcaatt ccattactgc ttgcatgcgc gcgaacactg
    26941 ctagtcattc ctgttttgat tccgtcataa atagtgtacg catcgattcc tagatatttg
    27001 acaatatagg aacggtcaaa tgtccgctgt agcaattggg gacggttggc ttcgctcaac
    27061 gctaaatttc ctgaaaaaat caatcctgaa agacaggtaa cagcgatagc catcggtttg
    27121 ctgacagggt tgcttttgat aggtattttc ttgaccttca accaaaccag tagcccaatg
    27181 aacacagcga tatccaacca atagatgaca tcatggggtt tcatcaaaga aaaggagctt
    27241 cctgacaacc cttgtgacac tttcgaaaag cctaatacag atttgactgt aatgaaatct
    27301 gtgaattccc gataaaaaat aatattcaag tacaacaaaa gcgtatttaa aatatcaatg
    27361 atcaaaagaa tggctaacgc tggctttggc tttttaatat acaaagaaag gccaaaaaac
    27421 aataaggtag tcgcaatcgg gttgatccac aagataaagt actggatcgt accttccaca
    27481 cctaatgaaa aatctgcata gtaggcaatg atcgtcttca gccagaaaaa gaaaacagct
    27541 gtcaaaacga ttgctaaacg attttggcac aattttttaa gttgtgtcac gtatatcact
    27601 tctctctatt cacatttttt cttattttgc tcctgaaaaa tgaaaatttc ctaaaaaagt
    27661 tgttatctta agattttttt attctttcta aagattattc cattcatttt tcacagcata
    27721 aaatcgtttc ggtgtaagcg gttaaaatag taaggagata aaaatcttgt ggtgaccgat
    27781 ttactctata gtattcaaaa gtagtttctc ggtattcgta caaaaaaaac atactaagat
    27841 tagaggggaa catcattgac gaatgatgtt ctcctctatt ttctattttt agattatagt
    27901 gaagagtgaa agaaagttcg aacaaagcct tgaggactac gagtccctat caaaaaccga
    27961 acttctttac tgtcatttta gaatcaggtt tacgtatgtt ggggccatga gcccgtgtat
    28021 aggaaattgg aatcgaaatg aagcagtaaa gttctttcaa cacttaaaaa ggaggaaaac
    28081 aaatgcctta cattcttgca ttagatgttt caatgggaca cagctacagt gtcctataca
    28141 aagacgacac ctgcttattt gaagacgaga tcgaacacac tcaaagagga tttcatgcat
    28201 tactcgaaga gatccagcac ttgcctgaac gtccattgat tgtttttgaa gcaacgggta
    28261 tttattccag accagtcgaa aagttctttc aagatcatca cttttcttac tgtttgttaa
    28321 acccgttaga agcgaaaaaa cagatggaag aaaacacctt acgcagttgg aaaacagata
    28381 aatcggatgc ccatcggctc gcacagactc atcttaaaaa tcaacgtcaa ccaaaagaag
    28441 tacaaagcaa tttttactta gagatgcgcg atttagcccg tttttatcaa gaaattgaga
    28501 agaaaatcac tcgactgcgc atggatttac acaactgcct tcagttgact tttccagagc
    28561 ttgaacagtt cttttcaaac aggctgaccc cttacgcatt gacattgatc cgtctttttc
    28621 cccatccaga ttttgtattg gcatctactc ggacaaaaat aaaaaataaa ctgatcaacg
    28681 aaacacggga aaaaatctcc gcgaatcgtg ccgagcaaaa ggcagatcaa atcatccact
    28741 atgcacagtg tgcctatcca gctgttgaaa aagacagtat tcactgtcaa aaaacgatct
    28801 attacgccga acttctccaa gatttactcg aacaaaaaaa agcactggcg acacaaatga
    28861 tcaaaaaagc cgaagcctct ccttgctttt tgttgtacca aacgttccct gggattggcg
    28921 cactcacagc cgctttattg ctcggtgaat taggagatat cacacgattt aaaacccata
    28981 aacaattgaa tgcctttgtc ggaatcgaca tccgacggta tcattcaggt aagtatactg
    29041 gccaagaccg catcaataag cgtggtaatc ccaaagcacg gaaaattatt ttcttcacca
    29101 ttcgaaatat gattcgccag caacgcgcag ctcctaatca tattgtcgat tattactaca
    29161 aattaaaaaa gcaacctatc cctaagaaag ataaggttgc cacagttgct tgtatgaaca
    29221 agcttctgaa atgtatgcac gctatggtta gggcacatac agagtacgac tatgcgtacg
    29281 cggtctcagt ggaccattaa ctaaaccact tacagtatac cacttctttg cccagatcga
    29341 aaatttttta cgaacggggc tttatttagt gtgccttgat tttgtcgctt tcgtttacga
    29401 gctatttttc ttgcaccttt tacttgacta atcgtaggaa agaggctgtg acaaaattct
    29461 tgtcgcagct ccctatttct tgatgcagga cagctttttc acaacctctc cactaaacgg
    29521 tgttcaatca gctgaccttc ggtattagct caaaaaacgg caaaatatga ggagctattt
    29581 tcccgttttt ttctctaata ctcaggtcaa accgctgatt tcacaacctc tgattcacgg
    29641 tgttccagaa gtagcttctt cggaaataag ccgaaatttt acaaaaactt gagaagcact
    29701 tttcgtaaat ttcttcttat ttcttgaagc tgaccacttc tgtcacaact tctgtttttc
    29761 attgtccata ggtttgatat tgtccttctg atgactcatt cgtaatgatt gaggcttgat
    29821 tttcttcatt tcccgttatc gtttgtccac tgccagaatc tgaagggctt agttctgtcg
    29881 actcgttttt ttgagaagaa gcagttgttt tcttgacttg ttcgttggtc tttttccatt
    29941 ccgctagtcg atcggccacc acttttttgt ccactttttc agaataagct gtaaccactt
    30001 ttccattttt caaacgtgct accttgtcgc catcatgagt caaaagcaaa acgaccaaat
    30061 gtttgtcttt gaattgatat tgtctgagag aaatcgcact aagtacgtca tcgatattga
    30121 taaattgatt ttcttctttt ccttttggca aatagatgtc caattcacct ttttcgtaat
    30181 tggcagttaa tttatattcc tcccaccaat aagttgagaa ttcttttttg aaattatcta
    30241 ctgattcttt gcttgctacg attttttctg tttctttctg agcaagatct gctgtcattt
    30301 ttcctgaaaa tgctgcggat aaaataagca gtaagaaaaa accgaataac caaaagagaa
    30361 gaaacggtct ttttttcctc atgccttcct ccactcctat ttaattttga ctattctatt
    30421 atatacaaaa aatgaaatag aaagacaaaa aagttgaaaa agatcgtaaa taattgaact
    30481 tctttttcct gaagcggtaa gaacgggaat tttcatacat tctctcttgg ctattcttta
    30541 agcttcaaat ccatttctgt ctgatacatc tttgaaccaa tgacccgatt tcttgatcgt
    30601 tctttttcca tcttgtgcta aatctactga aataaaaccg taacgattct tataggcatt
    30661 ggtccacgac cagttatcca tgcatgtcca catatgataa cctaaacaat tggcaccttc
    30721 ttgaatggct tgatggacat atttcagatg agattgaaca aattcgatcc gataatcatc
    30781 gtggatcata ccatcttcct tgataaatcg tgcttcattt tctacaccca tgccgttttc
    30841 agaaataaaa cagtcgatat tcccataatg atctcgtaca ttgatcaaaa tatcatagat
    30901 tcctttttca tagatctccc atccgcgata aggattcatt ttacgaccag gcatttcata
    30961 attgtcgaag taatcctcag gcataggacc gtttttagta tcgattggtg tttcttttgc
    31021 ttttattcgg cgtggttggt aataattaat tcccaaaaga tctactgtgt tttctttaat
    31081 gatcgtcaaa tcatctggat gaatctccgg cattagatcg agttctttta ttctctcaat
    31141 caattcactg ggaaactcgc cttttacagc tggatcaagg aatgaacgat tgaagaacgc
    31201 atctgctatt cgtgctgctt taacatcttc gggatttgat tcgtctcttg gataactcgg
    31261 tgtcaaattt aagatgattc cgatcacacc gctttgatcc atttcatggt aagctttgat
    31321 ggctaatgct gaagccaacg cttcatgata accaacttgg attgcctttt tcatatctga
    31381 ttcttccgga taatgccatc gatacaaata gcctgcctcg actggaacga ttggttcatt
    31441 atgggtaaac cattttttta ctttcgagcc aaacaattga aaacaacttt ttgcgtattt
    31501 ttcataggct tcaaccgttt ctcggttcaa ccatccgcct ttttcttgca gatacatcgg
    31561 catatcgaaa tgatacagat taacaaaggg ttcgataccg ttttcaatca actcatcaaa
    31621 atagtgatga tagaaacgaa cggcttcttc gttgattgtt ccagattcat caggtatcaa
    31681 acggctccat tggatagagg ttcgaaatga attatgccca gtctgtttca tcaactgtac
    31741 atcctcttga tacttcgtat aaacttgcga ggttttttca ggtcctactt gatcaaagaa
    31801 cttttgcggt tcgtttttgt accaataatc aaaaatattc tctccttttc catcgcctga
    31861 aattcttcct tctgtttgtg gaccacttgc tgcagagccc caccaaaaat tttttggaaa
    31921 ttgatatctt ttcatcaaag cacctctttt tcttttttag ttgattccat gtatgatatt
    31981 ttccatcaca atcacttttt cattttcttc ctcttctttt ttgattcgtt tcaacagctg
    32041 ttctgctgct cgttccccga ttttatctgt tggtggcgtg atcgtgattt ttggaaggat
    32101 actcagatcg tttttatcta aataatcata gtgactgaac gcaatatcct tcccgattct
    32161 gacaccttct tgattgaaac attcgatggc accaatcgac atgttgtaat tgctgacaaa
    32221 aatagcagtt gccccttttt ctaataatgc ttttgccccg taatagccgc tttttctaga
    32281 atagtctcct gtaaagatct ctaaatggat agacggatcc ttttgtaccg cttctttggc
    32341 tccagctaaa cgttctcttg ctacgtaatc tgtttgagcg gcagcaataa atcctattgt
    32401 tttatgtcct tgtccgccat gatgagcaat cacttttgct acactttctc gatcattcac
    32461 tactattgta tcaatacctg ctggcccacc cggacaattc aatgcgacaa ctgggatact
    32521 catttttgtc agcatttcaa atccagacca ctcttcttct gataagaaga cgatcaatcc
    32581 atcgatcgca tgcttcatca ggtattcgat tttttgttcg attttttctg gctgatcggc
    32641 aaacccgctt aataataagc tatacccatt ttcttcagct actctttcga tccctgaaac
    32701 aacctccgaa ccaaatcggt tagatatccc gtttatcaac agtccaatag agaaacttcg
    32761 attattcttc aatcctttag caatgatgtt ttctttatag tccaacgctg aaatcgcttc
    32821 tgatattttt tccatattct tttccttcag ctgctgaccg tttaaatagc gggaaactgt
    32881 tcccactgaa acaccagcca tcttagccac gtcacggatc gatgccatac attttcactc
    32941 ctgccagtta catttgattt ctttttcttg ttgttcgacc atgatgaccg ctttctttgc
    33001 ataatgccgc ttgtcatcaa tatccatata actaaagtct tcagaagtga gttcaaaaac
    33061 aagcagtctt tcctcttgag accgcaattc atctttacga aaagctttca ataatttcgt
    33121 tctttggatg acaacgccgt caattaatcg gacatagaca agagtacttg ttttacctga
    33181 aacacttcct gtatttttaa ctttaattga tattattact ttgtcaatat ccgttttgac
    33241 ttccatctga ctgtattcaa atgttgtata gcttagacca tgtccaaaag gataaaatgg
    33301 acttccgctt aaatcataat aattctcttg tttagaagca gcacgctgat agtaataaac
    33361 aggtagttgc cgactatttc taggataaga caaactcaat cgtccgcttg ggttatttct
    33421 tccagaaatc atctgcgcaa tggcttttcc accctcctgt ccaggaaacc atgctgttgc
    33481 aatggcattc gtaaatgact cgattttcat caaatcgtag ggtcttcctt gcacaagtat
    33541 cgtgatgatt ggcttgccta gttgatacaa ttgttcaatc agctgttctt ggtatccacc
    33601 taaagaaaga gaagcaacat ctacattttc tccagaatcc atgttgatcc ctttcgatga
    33661 aactgccccg ttacgaagaa attccatatc aaagttccgc gcacttgatc cacctagaac
    33721 tgcaatgatc acatctgagt tttgagcaag ggtcaccgct tctaataatt tttcttcctg
    33781 atcctcctga tttcttactt cacatccttg tgcataagaa acctttgatt ggggaaactc
    33841 tgtttttatc tcttgtacta tctttttttt caaacggtct tctgtttgtg gtgcagagta
    33901 gtctcccaac aagtggtaaa catcgtgagc atttggacct ataacagcaa tgttttttac
    33961 ttgttgatcc aacggcaata taccgtcatt ttttagtaaa gtgatacttt cttttgctgt
    34021 ctctaaattt aagtgctctg attcctccat gagatgatcc caatactcct ttggatcgtt
    34081 tgcaaatggc tgttcaaata aacctaataa aaatttgaca gataaaactc ggcgaactgc
    34141 ttgatctagt agcttttcat ctacgacttg attatcaatg gcttctgcaa tcttggtata
    34201 ggtctcatcc cacaaactga gatcgattcc tgccgctaat gcatatgctg cagcagtctt
    34261 tttatctgta aaaacatcgg ataaacgatc caaagccaca ccgtctgcca taacgattcc
    34321 ttgataaccg atctcttttc ttaatgtttg ctcaaaaagc gctcgattga tatgacaagg
    34381 tatcccgtcg atgtcattat aagcagccat gacacctact gcatttcttg tactcttcaa
    34441 taatggtccg taaatatcgg agaactctct gcgtccaatc gttactgtgc ctgaattatg
    34501 accgcctaaa gcttctcctt gtgcgataca gtgctttaac acaacaccga tctgatcctt
    34561 cttcttattg atttcctcta ctgtctgatc aagaaaagaa actttttctg aaataatctc
    34621 tccttggaac cctcgaacga ccgcttcaga aaaacgagct gagagaatcg gatcttcccc
    34681 aaagcactct tctgtccttc cccaacgagg atcttttgcc agatccaaag tcgacaccag
    34741 cgctaaatga acacctttag cagccagttc ttttgccatc aatcgactcg attgttctaa
    34801 taatgctaca ttgaaagtat ttccacggcc gatattcgtt ggataagaaa tcccatccaa
    34861 gccttgatga ccatgtggac attcttctac aagcaaaact ggaattttcc atcgcgaatg
    34921 ctgtagtaca tattcttgga ctaggttagc caccttccag ctttcttttg caggaatccc
    34981 attcttataa tctgttttag accacggatc agagcgaaac agaccgtata aggcacccag
    35041 accgcctccc catttaacat gttcttttaa cttttctgtt aattcgatct tgtctccgtt
    35101 tttttcataa cattcccaac catacaaatg ctggtttacc tgtcctactt tttcagcaat
    35161 attcatcctc gaaagtaaat cttctactct ttggcggatg gttaactttg ggttttgata
    35221 cgggtaatcg tacaccttaa tatcctcctt ctgccaattt tgccttatta gcggctaata
    35281 caaatggcag gaataaaaat acatctagaa tgataagtat tactgttaag actgctgctc
    35341 gtatatcgcc tcctgtagca ataaaagatt gtataaccgg tggagtgatc caaggtgcag
    35401 cagcaactgt tttactgatg attccaagac ttgtggctgc gtaggcaata accaagttaa
    35461 tagatggtat aatagcgcat ggaatcatat atattggatt catgactact ggtaatccaa
    35521 acataattgg ttccgtaata ttaaataaac aagtagtcag accaaattta gcgattgatc
    35581 gctgttcttg acgtttagaa gcccacaaga tagcaataac taacggtaaa atacacccgc
    35641 cacctccgat caatccaaat gcattggtaa agggctccgt aataatatta ggaatttctt
    35701 ttcctactga aaaagcatcc atgttttcct gaatagactg taacaaaggt gcttgccgta
    35761 taggagccaa aatactcgaa ccatgcatac caaacaccca taagaaatca gagaaaaact
    35821 gtaaaaataa gatgcctggg acggataaaa caaaagcttt caatggtgtc tgaatcgcta
    35881 attcaattaa ttgcggaaca ttcaaaccag tcgtatggtt taaagtaaaa acgacaactg
    35941 aaaaaaataa aatgacaact atttctggta ctaaagaatt aaatgacgca gctactgccg
    36001 gtggaactcc ttctggcatc ctgattttaa aactcttcat tttcgctaat ttgataaata
    36061 attctgtact gacaagggca gcaatcatcg ccgttgctaa gccagacgaa gaagtcagac
    36121 tttgtaagaa aacccctgta gcttctgctg tatttccgtc catcaaagtg actgtacttg
    36181 ataaagacat caaaataaac attacggctg tacttagaat tcctgcatga acaggactaa
    36241 atgatggatc atcaatcacg cctgtttgga tatagtgggt tgccaaattc cagccgatat
    36301 tgtaacacac gataatagct aaaatactca tcgtaccatt attcacgatt gaagcaagtt
    36361 cagccaatgg acttagatca agtattcttt ggactgttgc atttgaaaaa atcagagcat
    36421 tcagcaaaac catgacagaa gcagccatta ctaacggcat agtaacgata aaactgtctc
    36481 gaatggccag caagtgtcgc tggctactca tcttagatac gattggtagc attttttcag
    36541 atagtctgtc tacgactttc attatttcat ctcctagatt ctaaaattga accggttcat
    36601 ttttaatata gcactttttt taaagattgc aacacatttt tttaaaacgc tttctaaagg
    36661 tggtggattt tttatgactt ttgaaaaatt attctattta atggtatttt gtgaattttt
    36721 tctcttccat ccgtttgaag ttttactttc caatggtata atttctttat cctatcaaat
    36781 gggggaagta ttttgaagaa attaatcgta ctatttattt tgtctgcggg acttttgaca
    36841 ttgactggct gtgatttgtt tggcggctct gaccaatcct cggacgaaac aaccgattct
    36901 tctgtcttaa aagcggaaaa agcagatatc accaacagtg tatatacgct aaaagtacgt
    36961 aaagatatgg atttatccgc gggacagttg acatttacag ataaaaaaat ggaatggaca
    37021 cgcacgtatc agacacaagg agctgattct tctgaatcat ctgaagaaaa aacagtgctg
    37081 acagatatca agattactac caaagatgac gaatatatta tttcaggaaa agaaaataaa
    37141 aaagaagcaa cagtcacatt taccaaaatc ggtaattacc gtttgaagga taaggatggc
    37201 aacatctaca gtctatagtg ccatctgttg gatcttcttt tgtttctccc cttcttttct
    37261 gctatagtaa agaagcaaga tgactgtaaa ggagacgaaa tggatgatcg atagtcctga
    37321 ttattgtaca tgtaataaca acaaaaatat ccatacagaa tttaatgatc aaggaagttt
    37381 gagtatatgc gacaattgcc acaaactagt caaagattct tttaaatcgt ttgaatctta
    37441 tcctgtcaaa aattatgtaa aaagcaaaag aagataagca aaatcaagca aaactcacta
    37501 tggctgattt ttatgaactt caaactcagc ttcttccttt gatcaagctt caaaaactgg
    37561 aatcagtaaa acaaacagtg gatcatttca gttcacaaat caaagagtaa cgttttattt
    37621 atttcacatc tttgaatata aaataaccaa acgatagaaa gacttattct acatcacgta
    37681 gtaatttttc tttcccgtcg tttggttttt tacttttagc ctaattcttg acctttcgca
    37741 tgctgattat ctgtaaaata ttttccagca ggctttcctt ccaattcagc taatttttct
    37801 tggatctgac gaatcacctt ttttgcatct tctggatttt ctacaaaatc gaatgtatcc
    37861 ccatccactt gtattttagg acatacatca aaatcctcat accattgatt atagcgggtg
    37921 ttcagtgttt cgtaataggt gtataacgtt tcatcgcttt ctagctgttc atagtcacgc
    37981 ccgcgttttt tgatacgttc tagcatcgtg tccaaagaaa ctttgatatg gaccatcaaa
    38041 tcaggacgtt tttttggagc aacatcatct aactcgttta gcatcgtatc taataagcta
    38101 tcgtactgct gtacttcgat atccgttacc cgacctaaat cagcatttag atggaacaac
    38161 agactatctt cgtagatgga gcggtctagc acattgtcat cgttcactaa agcatttttc
    38221 attgctaaaa atcgcttatt caaaaagaag atttgcaata aaaatgcgta ctgctcagga
    38281 tttgaataaa aaagcggcaa tacttcgtta tcttcgacat tttcataaaa tggtttagaa
    38341 tcaatctctt ttgcaagcat atccgtcaaa ctgctcttac ctgcaccaat cgttcctgaa
    38401 atcaaaatca ctgacataca acccttcctc ttctataagt actcgcctac gtaagtttca
    38461 acaggcaatt tttcaaaatc atattcacgt acagcttggt ctgattttaa atacgtgtga
    38521 agactttgtg taatcatcaa gttcgtaggt accgtctctt cttgtacgat gatcatcggt
    38581 tttttcaaca tagttgccac acctaattcg taagccgttc ctggatcgat tgtttgatgc
    38641 tcaaagtcaa ggatagccaa aacaaattct gcatttgtca actcttccat atctttttcg
    38701 tatacttcct gcgcccatcc agcgctgaat agttcataat tgctttcttg gtgatgacgt
    38761 ggagagtaga aacttgtgac tgttttgttt tcctccaacg ctttttcgat acgacttact
    38821 cgatcgattt gctcctctga aaaaaatggt cctgctaaat atatttgact cattattttg
    38881 cctccttcaa taataactga tttttaatag atactgctcg taattcattg accagttcca
    38941 atggattgtt ggaaaagatc acttgatcca accatttttt atccataaaa tcctcttttg
    39001 caaaagtttc taataattgt tgcaagccgc tgtaataacc gccaatattc aatattccga
    39061 ttggcttatc aggtgtcagg ccgatcgcta tccaagaaag aagttgcgaa aattcctcca
    39121 atgtacctag acctccaggg aaaacgagga cagcatctcc tttttcaaat aataatacct
    39181 ttctttcatc aattgtctct gtaggaataa atgtagtaac atctttccca ggcagctcag
    39241 tttcaaaaag gccatttgga tagatacctg tgacttttcc gttattttcc agtactgttt
    39301 tactaatcac tcccataatc ccggagtatg aaccaccgta aaccagttcg atttcttctt
    39361 gtgccatata ttttcctaac gtttgcgcaa taaatttgta actttcttta ttgccaaaac
    39421 gggaaccgca aaaaacagta agtttcataa aaaaacactc cctaactcaa aagtctatct
    39481 tcattctaca cgtgtttttt taaaataaaa gactagattt tgtagttttt tagtggtata
    39541 tag
//
LOCUS       DAHUVV010000017        38338 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.017, whole genome shotgun sequence.
ACCESSION   DAHUVV010000017 DAHUVV010000000
VERSION     DAHUVV010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.017"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            161..391
                     /locus_tag="NK767_000883"
     CDS             161..391
                     /locus_tag="NK767_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCH6693896.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="NK767_000884"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="NK767_000884"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCH6693897.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="NK767_000885"
     CDS             1802..1978
                     /locus_tag="NK767_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCH6693898.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="NK767_000886"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="NK767_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCH6693899.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="NK767_000887"
     CDS             2593..3060
                     /locus_tag="NK767_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6693900.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="NK767_000888"
     CDS             3145..4029
                     /locus_tag="NK767_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCH6693901.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4143..4379
                     /locus_tag="NK767_000889"
     CDS             4143..4379
                     /locus_tag="NK767_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HCH6693902.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="NK767_000890"
     tRNA            4467..4547
                     /locus_tag="NK767_000890"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="NK767_000891"
     tRNA            4554..4625
                     /locus_tag="NK767_000891"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            4750..4920
                     /locus_tag="NK767_000892"
     CDS             4750..4920
                     /locus_tag="NK767_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693903.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5140..5847
                     /locus_tag="NK767_000893"
     CDS             5140..5847
                     /locus_tag="NK767_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCH6693904.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="NK767_000894"
     CDS             complement(5907..6551)
                     /locus_tag="NK767_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCH6693905.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6678..6965
                     /locus_tag="NK767_000895"
     CDS             6678..6965
                     /locus_tag="NK767_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693906.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7073..7393
                     /locus_tag="NK767_000896"
     CDS             7073..7393
                     /locus_tag="NK767_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6693907.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7474..8787
                     /locus_tag="NK767_000897"
     CDS             7474..8787
                     /locus_tag="NK767_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCH6693908.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATIQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8784..9503
                     /locus_tag="NK767_000898"
     CDS             8784..9503
                     /locus_tag="NK767_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCH6693909.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9631..10182
                     /gene="raiA"
                     /locus_tag="NK767_000899"
     CDS             9631..10182
                     /gene="raiA"
                     /locus_tag="NK767_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCH6693910.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10404..12938
                     /gene="secA"
                     /locus_tag="NK767_000900"
     CDS             10404..12938
                     /gene="secA"
                     /locus_tag="NK767_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCH6693911.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13064..14165
                     /gene="prfB"
                     /locus_tag="NK767_000901"
     CDS             join(13064..13135,13137..14165)
                     /gene="prfB"
                     /locus_tag="NK767_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCH6693912.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14270..14956
                     /gene="ftsE"
                     /locus_tag="NK767_000902"
     CDS             14270..14956
                     /gene="ftsE"
                     /locus_tag="NK767_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCH6693913.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14940..15833
                     /locus_tag="NK767_000903"
     CDS             14940..15833
                     /locus_tag="NK767_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6693914.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15985..17433
                     /gene="cls"
                     /locus_tag="NK767_000904"
     CDS             15985..17433
                     /gene="cls"
                     /locus_tag="NK767_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCH6693915.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17770..18627
                     /locus_tag="NK767_000905"
     CDS             17770..18627
                     /locus_tag="NK767_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCH6693916.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18742..19662
                     /gene="pstC"
                     /locus_tag="NK767_000906"
     CDS             18742..19662
                     /gene="pstC"
                     /locus_tag="NK767_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCH6693917.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19662..20546
                     /gene="pstA"
                     /locus_tag="NK767_000907"
     CDS             19662..20546
                     /gene="pstA"
                     /locus_tag="NK767_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCH6693918.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20561..21367
                     /gene="pstB"
                     /locus_tag="NK767_000908"
     CDS             20561..21367
                     /gene="pstB"
                     /locus_tag="NK767_000908"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCH6693919.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21383..22141
                     /locus_tag="NK767_000909"
     CDS             21383..22141
                     /locus_tag="NK767_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCH6693920.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22154..22831
                     /gene="phoU"
                     /locus_tag="NK767_000910"
     CDS             22154..22831
                     /gene="phoU"
                     /locus_tag="NK767_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCH6693921.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23069..24100
                     /locus_tag="NK767_000911"
     CDS             23069..24100
                     /locus_tag="NK767_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCH6693922.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24097..24864
                     /locus_tag="NK767_000912"
     CDS             24097..24864
                     /locus_tag="NK767_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCH6693923.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24964..26532
                     /gene="liaX"
                     /locus_tag="NK767_000913"
     CDS             24964..26532
                     /gene="liaX"
                     /locus_tag="NK767_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HCH6693924.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26829..27149
                     /locus_tag="NK767_000914"
     CDS             26829..27149
                     /locus_tag="NK767_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HCH6693925.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27149..27499
                     /locus_tag="NK767_000915"
     CDS             27149..27499
                     /locus_tag="NK767_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCH6693926.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27666..28604
                     /locus_tag="NK767_000916"
     CDS             27666..28604
                     /locus_tag="NK767_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCH6693927.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28619..29449
                     /locus_tag="NK767_000917"
     CDS             28619..29449
                     /locus_tag="NK767_000917"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCH6693928.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29493..30518
                     /locus_tag="NK767_000918"
     CDS             29493..30518
                     /locus_tag="NK767_000918"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCH6693929.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30533..31471
                     /gene="galU"
                     /locus_tag="NK767_000919"
     CDS             30533..31471
                     /gene="galU"
                     /locus_tag="NK767_000919"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCH6693930.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            31596..32036
                     /locus_tag="NK767_000920"
     CDS             31596..32036
                     /locus_tag="NK767_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCH6693931.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32039..32641
                     /locus_tag="NK767_000921"
     CDS             32039..32641
                     /locus_tag="NK767_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCH6693932.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(32704..33807)
                     /locus_tag="NK767_000922"
     CDS             complement(32704..33807)
                     /locus_tag="NK767_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCH6693933.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            34017..35018
                     /gene="ccpA"
                     /locus_tag="NK767_000923"
     CDS             34017..35018
                     /gene="ccpA"
                     /locus_tag="NK767_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCH6693934.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            35373..35768
                     /locus_tag="NK767_000924"
     CDS             35373..35768
                     /locus_tag="NK767_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693935.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            35768..36019
                     /locus_tag="NK767_000925"
     CDS             35768..36019
                     /locus_tag="NK767_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693936.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            36062..36457
                     /locus_tag="NK767_000926"
     CDS             36062..36457
                     /locus_tag="NK767_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693937.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            36454..37710
                     /locus_tag="NK767_000927"
     CDS             36454..37710
                     /locus_tag="NK767_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HCH6693938.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            37722..38045
                     /locus_tag="NK767_000928"
     CDS             37722..38045
                     /locus_tag="NK767_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693939.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            38038..38304
                     /locus_tag="NK767_000929"
     CDS             38038..38304
                     /locus_tag="NK767_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693940.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        12860 a         6514 c         8261 g        10703 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtc
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6601 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6661 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6721 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6781 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6841 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6901 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6961 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     7021 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     7081 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7141 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7201 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7261 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7321 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7381 cataggtgaa taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct
     7441 tgttttttcg tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt
     7501 ctgacgagag atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag
     7561 atcgcagcag tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg
     7621 tccgtcattg atggacagct tgcagattac ggttattttt gtacacattg tctatattta
     7681 gggcgatgcg acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga
     7741 gaagtcgtct ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga
     7801 atactttgcc attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa
     7861 acagagatgc tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt
     7921 tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca
     7981 gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt
     8041 gttatctgta cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc
     8101 gatgagatcg atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca
     8161 gcgattaaac cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta
     8221 aaggaaatca agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta
     8281 cttcctgaac ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag
     8341 aagataaaat ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta
     8401 ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca caattcaaga ggctcttccg
     8461 agatatgaga tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa
     8521 atgcgagaag caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca
     8581 tttgaaagaa tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct
     8641 ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac
     8701 tttttctatg aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg
     8761 aatgaagaag ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa
     8821 atttaacatt cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag
     8881 tttgtcgcgc acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa
     8941 tccatttcaa tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg
     9001 actgtcaaga atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg
     9061 tgtacgatga aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac
     9121 tagcaggcac ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct
     9181 attgtccaat acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg
     9241 agatgttgaa agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc
     9301 tggctcaagc aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg
     9361 cagtcacctt ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa
     9421 caggacagac tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc
     9481 gaaccatttc tttagcaaga tgaattagat gtggaaattt gatagcgatt taatttgcaa
     9541 ataacctaga gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct
     9601 tgattgtggt agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat
     9661 atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc
     9721 tatttcagtg acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa
     9781 acagcaaaag tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact
     9841 tctccagatt tatacgcaag tatcgatttg gttgttgaca aattggaacg ccaaatccgc
     9901 aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct
     9961 actgctttat tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt
    10021 acgaaacgcc tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg
    10081 ctgggacata acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac
    10141 cgccgtaaag atggaaaata cggactgatt gaaacagact gatcagcctt acttaaataa
    10201 caacgaaaac ttgcagcagc tactgacagc tgccgcaagt ttttttgagt ttcctcctct
    10261 tttagatgaa atatgaataa attatgaagt tcttacttta atacgcttaa gatatttcaa
    10321 ttatctataa ctaatgataa aatgaaaagt aaagtctaca tagtctggat aatacacaga
    10381 aggaaaagga gattacacaa cgcatggcca atttgattcg ttcacttatc gaaaacgata
    10441 agaaagaact aaaacgttta gatagcattg caaaaaaagt tgaagcatac gctgaccaaa
    10501 tggcagcatt aactgatgaa caattacagg caaaaactgc tgaattcaag ggcagatacc
    10561 aaaaaggaga aacattagat caattacttc cggaagcttt tgcagtcgtc cgagaagcag
    10621 caaaacgtgt attggggtta tatccacacc acgtacaatt gatgggtgga atcgtgcttc
    10681 acgatggaaa tatccctgaa atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc
    10741 cagtatatct aaatgctctg actggcgaag gggtacacgt agttaccgtc aatgaatatt
    10801 tagcaacccg tgactcgacg gaaatgggcg aactttataa tttcttagga ttaacagtgg
    10861 ggttgaatat caactcaaaa tcttcggatg aaaaacgcga ggcttataat tgtgatatca
    10921 cttatagtac gaataatgaa ttaggatttg actacttgag agacaatatg gtcgtttacc
    10981 gccatcaaat ggtacaacgt ccgctgaatt atgccattgt cgatgaagtt gactcaatct
    11041 tgattgatga agcgagaacg ccgctgatca tttcagggca agcagaaaaa tccacagctt
    11101 tatatacaag agcagacaac ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg
    11161 atgtacaatc aaaaacaatc agtttaaccg aagcagggat cgaaaaagca gaagaaacat
    11221 tcggtttgga taacttatac gacattgaaa atactgcatt gacccatcac ttggatcaag
    11281 cattgagagc caactatatc atgcttttag atatcgatta tgttgtccaa gacggaaaag
    11341 tactgattgt tgaccagttc actggtcgga tcatggatgg acgccgttat tcagatggac
    11401 ttcaccaagc aatcgaagct aaagaaggcg tagagatcga agacgaaacg aaaaccatgg
    11461 cgacgatcac tttccagaac tatttccgta tgtacaagaa acttgcggga atgactggta
    11521 cagctaaaac agaagaagaa gaattcagag aaatttataa catccaagta taccagattc
    11581 cgacaaatcg tccggttatc cgtgatgatc gtccagattt gctttaccca acattacaaa
    11641 gcaagttcca cgctgtcgtg caagatatca aagatcgcta ccgcaaaggt caacctgtgt
    11701 tagtcggaac tgttgcggtt gaaacatctg agttattatc agatatgctg aatcaagaac
    11761 gtattcctca tgaagtgctg aatgcgaaaa atcactttaa agaagcagag atcatcatga
    11821 atgctggtca aaaaggtgct gtgacgattg ctaccaacat ggctggtcgg ggaacagata
    11881 tcaagttagg acttggggta agagaacttg gtggtctagc agttatcggg acagaacgac
    11941 atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg
    12001 gtatgtcaca gttctacttg tcattggaag atgatttgat gaaacgattc ggttcagaac
    12061 gaatcaaatc tttcttagat cggatgaaga tcggtgagga agatgctgtt atccaaagca
    12121 agatgctgac gaaacaagta gaatctgcac aaaaacgtgt agaaggaaat aactatgaca
    12181 cacgtaaaaa cgtcttacaa tacgatgatg tgatgcgtga acaacgtgaa gtaatctata
    12241 aacagcgaca agaagtaatc atggaagaaa aaagcttatc caagccacta atgaacatgg
    12301 tcaaacggac aatcagtcgt gtagtagatg cgcatacaca attagagaaa gaaaattgga
    12361 atttggaaag tatcatcgat tttgccggca gtactcttgt ccatgaagat agtattactc
    12421 ttgcagatat cgaaggaaaa acagctcagg agatcaaaga gtatctaaac gaacgagcac
    12481 aagaagtatt taataccaaa gcggctcaac taaatggtcc agagcaactg ttggaatttg
    12541 aaaaagtcgt gatcttgcgt gttgtggact ctaaatggac agaccatatt gatgcaatgg
    12601 atcaattacg tcagtcaatc ggcttacgtg cttatggaca gaacaatcca ttagtcgaat
    12661 atcaaacaga aggctataag atgtttgaag atatggtagg cgccatcgag tatgaagtca
    12721 ctcgaatctt tatgaaagct gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag
    12781 gaacagcttc tcactcagaa gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa
    12841 aacagccaat ccatgtgaac aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga
    12901 aaaaatataa gaactgtcat ggaaaaaatg caaactaatt tctgacagca ggattggtga
    12961 gacaaattct ttttttaaga attcgtctca ccttctttct gttatttgct gattctgtta
    13021 gactagatat aatgaaaaaa gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat
    13081 tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc ttcaggaggt ctctttgact
    13141 tagaccagct ggaagaagac attgcggaag cagaagcaaa aatggctgaa cctggttttt
    13201 gggatgatag cgaagctgct caaaaagtga tcagtgaaaa caatgcaaat aaagaaactt
    13261 atgatcaatt ccattctctt gctgaagaac tggaagaaat cgaattgatg gtggagatgt
    13321 tgcaggaaga acctgatgca gccatgcaag aagaagcaga aacacgtatc aatgcgctag
    13381 atgaaaaaat gaagacatat gaattatcta tgttgttaga tggtccttat gatcgaaaca
    13441 acgccatcgt tgaattgcat ccaggggctg gcggaacgga gtcccaagat tggggcagta
    13501 tgcttttacg tatgtataca cgctgggcag agcagcatgg gtatcaagta gagacactag
    13561 attatcaagc aggtgatgaa gcaggaatca agagtgtgac cctactgatc aaaggacaca
    13621 acgcatttgg ttatctgaaa tcagaaaaag gtgttcatcg ccttgtacgt atctcgcctt
    13681 ttgactcagc gaaacgacgt catacttctt tttgttcggt agatgtgatg ccagagcttg
    13741 atgaaacgat cgatatagaa atcaatccag atgatttgaa aatcgatacg taccgagcaa
    13801 gtggtgcagg tggtcagcac atcaataaaa cagaatctgc tgtacggatc actcacttgc
    13861 ctacaggaac agtagtagct agtcaggcac aacgttcaca attgaaaaac cgtgagcaag
    13921 cgatggggat gttaaaagct aagctttatc aattagaagt ggaaaagaaa gaacaagaag
    13981 cagctgcact tcgaggagaa caaaaagaaa tcggctgggg ttcacagatc cgttcttatg
    14041 ttttccatcc atattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc
    14101 aagcagttat ggacggtgac cttgatttat ttattgacgg ctatttaaaa atgcatctac
    14161 aataagaacc cattgttaaa ttgaaataaa attgtaagat tttgcaagca tgttgaaaca
    14221 ttgccatgct ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat
    14281 gaaggatgta atgaagaagt actcaaatgg tacgaccgct atccgcaatc tctcaattgt
    14341 catcgaacaa ggagagtttg tctatgtggt tggtccgtct ggagccggta aatctacatt
    14401 catcaaattg atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca
    14461 tgatctaata gcaatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt
    14521 gttccaagac tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgctat
    14581 gcaagttatc ggcagaaaac ctcgggatat caaaaaacga gtaatggaag tgctggacct
    14641 tgttggtttg aaacacaaag ttcgagtctt tcctagcgaa ttatccggtg gggaacagca
    14701 acgtgtctct attgctcggg caatagtgaa tacgcctaaa gtacttatcg cggatgaacc
    14761 gacaggaaat ctggatccag aaaactcatg ggaaatcatg aaattattag atcggatcaa
    14821 tgcccaagga actactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg
    14881 gcaccgagta attgctatcg aaaacggccg cattattcga gatcaagcgg aaggggaata
    14941 tggatacgat gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac
    15001 gtaacggctg gatgacagtt gcttcagtca gtgcggtaac gatcacattg actttagtcg
    15061 gtgtattcat ggcagttatc atgaacgcaa ctaaattagc tcaagatata gaaggaaacg
    15121 ttgatgtctc tgtattcgta gatatcggta caaaagacga agagatgaaa aagctagaaa
    15181 aagaattgaa tgacttagat catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat
    15241 tgaaaaaaat ccaaaacgaa atgggtgatt cctggaaatt gtttgaaggt gacaataacc
    15301 cgttatatga tgtgtatgta gtaagtgcta ctgatccttc ttatacaaaa caagtagcag
    15361 aagaagcagc agatttgaag aatgtttctc gttctgatta cggtggagct tcctcagatc
    15421 ggattttcca aatcgctggt gcagttcgga catggggatt agttgcagcg gcattgctgc
    15481 tgttcgtagc gatgtttctc atttcgaaca cgattcggat cacgattttg tctagacagc
    15541 gtgaaattca aatcatgcgt ttagtcggtg caaaaaatgg atatattcgc tggccgtttt
    15601 tccttgaagg tggctggatc ggtctgatcg gtgcgattct tccgattttg atcatcacat
    15661 ttggttatgc atggtttttc caattgatca atccatcatt attacgttcc cattattcat
    15721 tgattcatcc acaaaatatc gtttggaaaa tcaatctttt aatggtcggc attggtgtga
    15781 tcattggttc acttggctcg atcatttcta tgagacgttt cttgaaattc taataccaca
    15841 atataaataa aatgagaaag ttggacagtt tcgttccagc tttctttttt tattggctag
    15901 gagaatagaa ggaagcggct tttttccctt tactttaaaa aacaacttgg gtattcttaa
    15961 gttggtagaa gaagaggtga agccgtgaaa attatcgttg acaatttttt tacgatcctt
    16021 ttgattatga atattttgct ttcctttatc atcgtttttc gagaaagaaa ggaaactgct
    16081 caaacatggg catgggtgct cgttttgatg ttcattccag ttgtaggatt tattttatat
    16141 atttttttag gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa
    16201 atcggtaaga acctggaaat tgaagaagac aaacaggcat tgatgcgagg gctttatcct
    16261 catccgccta ctggacaagt agatgtgaaa caattgatct atatgctcac ggtatttgaa
    16321 agtacgctct atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc
    16381 gatgcgctga ttgaagatat caagcaggca gaagaccata tccacttcca gtattatatt
    16441 tatcgcagtg atgcattagg tgaagaagtt cgagatgcat tgacagaggc ggcaagacga
    16501 ggggtaaaag tcagagtcct tcttgatgca tggggttcca cacaagtttc ttccagcttt
    16561 tttcagaatt tgaaaaaagc aggcggagaa gtcgcatttt tctttcctct atttgttccg
    16621 tatattaatc ctcggatcaa ttatcgcaat catcgtaaaa tcgtagttat tgatgggaaa
    16681 atcggatata caggtgggtt caatgttgga aatgaatatc taggattggt aaagaaattt
    16741 ggttattggc gagacaatca tttgcgtatt tacggagaag cggtgtatac cttgcaaaac
    16801 cgttttttga tggactggaa ttctcagcat acaaaagaag taggctattc tcccaaattt
    16861 tttccgtcca ttcactctac tggtgatata gctgttcaaa tcgtgacaag cggaccagat
    16921 accgaacatg aacaaatcaa aatgacttat ttgaaaatga tttcgttagc caaacgggag
    16981 atattgatcc aaacgcctta ttacataccg gatggatcga ttcatgaagc actgaagttg
    17041 gctcttcttt caggcgtaaa agttcatatc caaataccta acaaaccaga tcatcttctc
    17101 gtttactggg caacgtattc tttcgctgca gagctaatcg agtatggagc aaggatcgaa
    17161 acatatgaga atggatttat tcatgcaaag acgatgatta tcgatggtgg cattgtttca
    17221 gttggctcag caaatatcga tgttcgttca tttcgcttgg actttgaggt caatacactg
    17281 gtttatgatg aacgaatggc ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt
    17341 tctacgcatc tcacaaaaga aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa
    17401 ggattagcaa gattgatttc gccgctcctt taaaacataa aaaggacatg agaaccatca
    17461 acagatggag atctcgtgtc cttttctaac ggggccattt ctgagagtac gtcagaaatg
    17521 gagaaagcgt ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag
    17581 caagaaataa gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag
    17641 aaacttttaa tgagatagtt ttttttacaa ttatgagaat ttattttgtt atttaatgat
    17701 tttgattcgt tttcatcaag taatgaaaat gataaaataa acatacttta ggattaaagg
    17761 agatttctaa tgaaaaaact taaattattc ttaccaataa taggattagc tattctttta
    17821 tcaggctgtt caaagtggat tgatcgagga gaatccatta cggctgttgg ctcttctgct
    17881 ttacagcctt tagtagaaac agttgctgaa acttaccaga cagaaaatcc tggaaaattc
    17941 atcaatgtac aagggggcgg aagcggaact ggtttgtctc aagtacagtc aggagcagtc
    18001 gacatcggaa actctgattt gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta
    18061 gtcgatcatc gagtagcagt tgttgggatc actcctattg tgaacaagga tattggcgta
    18121 aaagacatca gtatggaaaa cctgaaaaaa ctatttacag gcgagatcaa aaactggagt
    18181 gaagtcggtg gaaaagatca gccagtagtt atattgaacc gcgcttcagg aagcggaacc
    18241 cgtagcacgt ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa
    18301 gattcaagcg gaatggtgcg ccagatcgtt gccgacacac ccggtgcaat cagctatgtt
    18361 gctttttctt atgtttccga tgatgtagat acgttgaatg ttaacggtgt agaaccgaca
    18421 gatgaaaatg tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga
    18481 gatcatccaa aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa
    18541 gataacatcg ttggagaact aggttatatc cctgtttctc atatgaaagt cgaacgtgat
    18601 tggcaaggaa atatcgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact
    18661 tttacatgtt attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac
    18721 ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa
    18781 cgttcaaaaa tggaacagcg tggaaaattc atcagttttt tatgtatcgc gctgatcgtg
    18841 ttggttgtat tatctatttt ttattttgta gctagtaaag gattggccac cttttttact
    18901 gataagatca atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatcgga
    18961 gcaaatggga aaccaatggt aggggcattg cctatgatca caggttcgtt tattgtgacc
    19021 tttttatctg ctatcgttgc cacgccattt gccattggtg cagcagtatt catgacggag
    19081 atctctccta aaaacggaac gaagatctta cagccggtca tcgagctgct tgtaggtatt
    19141 ccttctgtcg tttatggttt tatcggttta tcagttatcg tgccttttgt cagaagtatt
    19201 tttggcggga ctgggtttgg tatcttagct ggtacatttg tgttgttcgt tatgatcttg
    19261 ccgactgtca caacgatgac tgttgatgca ttaaaggcag tgccacggca ctatcgggaa
    19321 gcttcattag cgcttggtgc cacaagatgg caaacaatct acaaagtcgt gctacgagca
    19381 gccgtgcccg ggattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc
    19441 ttagctatcc aaatggtcat cggtaatgca gcattgatgc caacaggtct tactactcct
    19501 gcttctacat tgacgtccat cttaacaatg ggaatcggaa atacaatcat gggaacagtt
    19561 gaaaataatg tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat
    19621 atcgtgatcc gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag
    19681 ataaaattgc tacaggtgtt ttatatatag tttcggggat catcgtattg attttagcag
    19741 ctttactact ttatatctta gttcggggaa taccgcacat ttcatggtcc ttcttgaccg
    19801 aaccttccaa agcctaccaa gaaggcggtg gaatcgggat ccagctattt aactcgattt
    19861 atttgctttt gatcacgatg atcatcagtt ttcctatttc tttaggtgcg ggtatctatc
    19921 tttcagaata tgctggaaaa aactggctaa cagatgtgat tcgcacgtcg attgaaatct
    19981 tgagctcgtt gccttctgtc gttgtcggtt tattcggttt cttggttttc gttattcaat
    20041 tccagtatgg tttctcgatt atttctggtg cattggcttt gactgtattc aacttgcctc
    20101 ttttgactag aacagttgag gagtctttac aagcggtcca ttacacacaa cgagaagcag
    20161 gattggcctt aggtttgtca cgctgggaaa cagtgataaa agtggtggtt ccagaagcga
    20221 caccagggat tttgactggt gtgatcctaa gttctggaag aatttttggt gaagcagctg
    20281 cattgatcta tacggctgga caaagtgcac ctgcgttaga ttttacgaac tggaatccat
    20341 taagtgtttc aagcccgatc agcattttcc gtcaagctga aacattagct gtccatattt
    20401 ggaagatcaa tacagaaggc acgatgcctg atggtgtttc tgtttcggct ggcgcttctg
    20461 ccgtgttgat tattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat
    20521 tataccgaaa aatgacttca gcctaaggag aagaagcaaa atgaaaaaat ataactggaa
    20581 tgaaacaaat gtcatcacca tgccaaaaga gaaagaagta gcactatata cggatgatct
    20641 acatgtctgg tatggcgaca atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa
    20701 taagatcact gcactgattg gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt
    20761 gaatcgaatg aatgatggga tcgctaatac gaaaatcact gggaaaatca tgtatcaagg
    20821 cgtagatatt aatgcgcctc aagtggatgt ttatgagatg cgcaaacgga ttggcatggt
    20881 ttttcaacgt cctaatccgt tcagcaagtc gatttatgac aatattacat ttgctttgaa
    20941 acagcacggc gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca
    21001 agctgccttg tgggaccaag taaaagacag cttggacaaa agtgccttgg ctttatctgg
    21061 cgggcaacag caacgtttat gtattgctcg tgcgatcgcg atgaaacctg atatcttgct
    21121 gctagacgag ccggcaagtg ctttggaccc tatttcgaca ggaacggttg aagaaacact
    21181 tgtaaacttg aaagaagact atacgatcat catcgtgacg cataacatgc aacaagcagc
    21241 tcgtatcagc gactatactg cctttttcta tttaggaaaa gtaatggaat atgacaaaac
    21301 taacaagata tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt
    21361 tggttaagac ggaggcctca acatgacaaa agagattatt tcatctaaag atcttcatct
    21421 gtattatgga aagaaagaag cactaaaagg gatcgatcta agcttcaatc aaggggaaat
    21481 cactgcgatg atcggtcctt ctggttgcgg aaaatccact tatctgcgtt ctttgaaccg
    21541 gatgaatgac ctgattccga atgtaacgat caccggaagt gttttatata aagacaaaga
    21601 catctatggt ccaaaaacag atgttgttga actaagaaaa gaaatcggca tggtattcca
    21661 gcagccaaac ccatttcctt tttctatcta tgaaaatgtc atctatggat tgaaattaaa
    21721 aggggaaaaa gataaaaaag tccttgatca agtagttgaa gaaagcctga aggcagcgtc
    21781 tgtttgggat gatgtgaaag acaaattgca taaaagtgct ttatctttat ccggtgggca
    21841 gcagcagcgt gtatgtatcg cacgtgtttt ggcagttaat ccggaaatca tcttattgga
    21901 tgaaccaaca agtgcgcttg atcctgtttc tactgggaaa atcgaaagta tgctgttaga
    21961 attaagagag cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat
    22021 ttcagataag acggcattct ttttagatgg tcacttgatc gaatacaatg atacgaagaa
    22081 gatattcatg aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata
    22141 aggaggaaac ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca
    22201 gttctacgaa atggggatga tggtgagcag cgccgtgcac aagtcagttc gtgcatacat
    22261 caaacacgat aaaatcttag cccaagaagt tatcgaaaac gatataaata tcaataacat
    22321 ggaaacacgc ttggagaaga aaagttttga aatgatcgca ttgcaacagc cagttacaac
    22381 tgatttgcgt atgatcatta cggtcatgaa agctagctcg gatttagaac gtatggccga
    22441 tcatgcggtg tctgttgcta aatccacgat tcgcgtaaaa gggcagacga gaataccgga
    22501 aatcgaaaaa gagatctccg atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct
    22561 agttgcttat gtcaaaacag acgaaaaaga cgcacgtacg atcgccaata tggatcaaag
    22621 agtcaatgac tatttcaatc gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc
    22681 tgagacagtc atcagtggaa cagactactt gaatgttgcc agttatctag aacggatcgg
    22741 agactatgtg acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga
    22801 gttaaatacg aaccacaatg agattttcta aactgaagct gtgattatgc cgtttgttta
    22861 ggaaaagagg ttgtgaaatc agcggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    22921 cagtcaaaca aaaaatagct cctcatattt ttcgtttggc tggacttatt accgcagatg
    22981 caagcttttg aacaccgttt attcgggaaa gggtttgtga caaaacatgt cacaaaccct
    23041 tttcatcatt tcatagaaag gataaagaat gcaaaaaaaa attagcatca tcactatatt
    23101 ttttatttgt gtgcttactt catgggttat tcagcatcaa gccgctttat atgaagtacc
    23161 tgtcatcgag atagaaaaag tacgaataaa agaggatcat caattgatta caggaaaact
    23221 gatgaacacg gatcaaaaag gagaatcgat ttatttatcg gttccttatc aaaaaaatca
    23281 aatagaaaca gcaaatataa aagcgagaca aatgtatcta gtccaaatgc atggagatga
    23341 atggcagcta gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa gtatcttcat
    23401 tggcgccatg ttgcttattg gtggaaaaca gggtctattt gctttaggaa gtctcatcat
    23461 caatatcgtt ttacttttac ttttgttgct catctttact aaaacagaag ggatttctct
    23521 attaaatata tctatcctgt tcgtcgttat agggatgatt attagcatat tggctttaga
    23581 tggatggaat agaagcagta taatcaaaat ttcagctgca gttacgagtg tttttttagc
    23641 attttttatt tgtcttttga caatgaatca ttggaaagac caaggactgc gctatgagga
    23701 actgaattat ctgacacgac cgtatcgttc tgtttttctt tctagcgtac tgattggaac
    23761 agctggagga acactcgata ccgttatcac agtcattgcg acattagaag aattgactaa
    23821 gcgacaacca gaaataacag cgaaacagtt atggctttca ggaaggcaag tgggtcgaga
    23881 tgtgattggc tcgatgacca atatcctttt gttcgtctat atcagtgggt cagttccatc
    23941 gcttctttta tatttaggaa atcaatggtc gttcaaagaa acgatagaac agcatttatc
    24001 attggaattt ttacgcgtca ttgctggcag cttaggaata gtattatcga tccctatctc
    24061 ggtcctcttt tttctgactg taaggaggct gaaacgatga atgtacttat tatacttgtt
    24121 gggattttag caatcagcgt gctacttatt ggcggtacgc aaggtatgta tattctttta
    24181 ggcttattca tcaatttagg gatctttttc ctgttgttat tcggttatca tcagaagtgg
    24241 ccgatactgg cattgtcggt cataggattt ttattgattg ctgtggttat cttatttttt
    24301 atcaacggtt ataatctaaa aatgcgtgca gcttttgtga gtatactgat ctttttattt
    24361 tgtttcctac tattgcttcc tatcactgat tttttagcaa tccaaggttt tacatcggta
    24421 gaactagaag aattatctgg cttagataag actttagcga ttgattttag attacttacg
    24481 agatcgttgc ttctgattag tcttagtggt gctgttttag atgcgagtgt agcaatttct
    24541 agtgggacat tcgaagtata tcaggcaaat ccgcaattat ctttttacca attgcgccat
    24601 tctagtttcg ctattgctaa aaaaatattg gcgtcaacag taatgacact tctttttgca
    24661 ttcatgggaa gttctttagc gttgatcctc tggtttattg atttagacat tccttttcag
    24721 caagtgatca atgaaaaatc atttgttcta gaatatacga tggctattct taccacattg
    24781 tccgcactac ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag
    24841 aaagaacaac tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa
    24901 tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt
    24961 tccatgaaag aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa
    25021 gaaggcttag atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa
    25081 gaagcagaca aggtaactgc tagtcataaa gaaaaagatc aggcaagtca attgatcgat
    25141 aaacttgaaa acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga
    25201 caagatcaag aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact
    25261 tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag
    25321 aaacaggaag cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa
    25381 gaattagcac agcgtcagac gttagaagca gaaatcaaag aattagaagc tagtggcgat
    25441 acactgttag aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa
    25501 tggtctgaga aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat
    25561 caagcaacag atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta
    25621 ggcaaattct tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa
    25681 tggaaagatg tcagcttacg tgtccctggg attgcgacaa cgaaatttga gcatgaattt
    25741 tactatgaag ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg
    25801 ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa
    25861 atgggtgctg aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat
    25921 catatctcct tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct
    25981 aaacgagtat acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca
    26041 ttggaagcta aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta
    26101 actgcaacga tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac
    26161 atcttagatt ctattatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa
    26221 aacctttctg tgtctttggt aaatggtgat gtacgtctta cgattaaaga agataatttg
    26281 aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc
    26341 ggtttggaag gacatgctaa aacaagtctt ggaagtatca acagccgtct gtccaattac
    26401 gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca
    26461 gatggtgaaa tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat
    26521 acagataaat aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt
    26581 gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt gccgatagtc tatctcttga
    26641 acaccgttta ccaagagatt atgaacctgc caattatcgc cgagtcacaa ggaataattt
    26701 cagaaaaagc tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat tttgcagaaa
    26761 gacaccgatt aagaagaagg ttgtcacaac gattttcttc atttaaaata gtagaacaga
    26821 ggtgagacat gatgaaaaaa aagttgacaa aatctaataa aaatgttgtt ttgacaggga
    26881 ctttagcagg aataggagaa tacatcggga ttgatccgac tgttatccgt gtgctttatg
    26941 tattcctgag cttagttgct tttggaagtc caattatttt atacatttta ttagctttga
    27001 ttatcccatc tgcgcgttct gcacgaacaa actatggaca taacaatgca tattatgaaa
    27061 aaaacaatta tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa
    27121 tcgatgatga cgaatggagt gacttttaat gtcgtacttt caacgtattt tagttaatac
    27181 gttaacattc atatcattgg ctgttctttt tcctgcgatg gtccatgtga gcagtatttg
    27241 gacggccgtt cttgccagct tcgtattgtc tgtgttgaat atgtttgtca aaccgatttt
    27301 gacgatactg tccctgccct ttacattagt cacattggga ttgtttacgt ttgttatcaa
    27361 tgcagcgatt cttaaaatga cctccttttt tgtgggagca gaacatttta gtttttcttc
    27421 attcggtgca gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa ttgtcagcga
    27481 acataacagt agaaaataaa agaaagctgt gccgaactat cggcgcagtt tttttattta
    27541 gaatcaggaa tgcgttttct attacattgg taattaaata aaaacgtttt ttttaattgg
    27601 tagtactttt ggctagacaa tgataaaatg atagagaaca ctctaataga aatgaggtag
    27661 catgaatgtc agaagttgta aaagttagcc aattagtgaa aaaattatct ttggaaatcg
    27721 tgacaggcga cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc tcacgcccag
    27781 gactagagtt gactggttat tttaattatt attcccataa tcgtttacag ctatttggca
    27841 gtaaagaaat cagttttgcg gaacggatga tcccagaaga gcgtttgatc atcatgagaa
    27901 gattatgtag tgaagatatg cccgcattta ttatttcaag aggattacca gctccagaag
    27961 aaatgatcca ggcagcaaat gaaaatggga tagctgtact gcgttctccc atctcaactt
    28021 ctcgtttatt aggagaatta tccagttatt tagatggtcg ccttgcccct cgaacaagtg
    28081 tccacggtgt tttagtggat gtttacggat taggtgtact gattcaaggt gacagtggga
    28141 taggaaaaag tgaaacagcg ttggagttga tcaaacgcgg ccatcgattg atcgcagacg
    28201 atcgagtaga tgtttatcag caagatgaat taacggtgat cggagaaccg cctaaaatcc
    28261 tggaacatct gattgaaatc cgaggaatcg ggatcattga tgtaatgaat ctttttggtg
    28321 cgagtgcggt acgtggctct atgcaagtcc agttggctgt ttatctggaa gcatgggcaa
    28381 aagataagaa atacgaccgt ctgggctctg aagacacgac cgttgaaatc gcggaagtgg
    28441 gcattccaca aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc atcattgaag
    28501 tagccgcgat gaatttccgt gcacgtacaa tgggctttga cgcaacgaaa acatttgaag
    28561 aaagattaag tcgcttgatt gaagaaaact caggcaacga ctagcaagga gtgacaatat
    28621 gttagcacaa atcaatcgag ttgcttttga gcttgtaggg attcctatct actggtatgc
    28681 attgatcatc gtatcaggta tcattatcgc tatgtggctt agcagcagag aggctgttcg
    28741 ggtaggatta aaagcagatg atgtgacaga ttttatgttg tggggattgc ctatctccat
    28801 tattggcgct cgcttatatt atgttttatt tgatcttccc cagtatatag ctgatccaat
    28861 ccaaatattc aatatccgtt caggaggctt agctatttat ggcggactga ttgcaggcgc
    28921 gatcacgctg tattttttta ctaaatatca ttttatttct ttgtggactt ttttagatat
    28981 cgctgcacca agtgtattat tagcacaggc aatcggtcgt tggggaaatt tcatgaacca
    29041 cgaagcatat ggaccagaca cgacgcgaag cttcttagaa agtctgcatt taccgcaatt
    29101 catcattgat aatatgtata tcgaagggat ctatcgtcaa ccaacatttc tttatgaatc
    29161 actttggagt ttgattggat ttattttgct tttgttttta cgaaagaaac cacacctttt
    29221 gaaaaaaggc gaagtccttt tatgttatct aatgtggtat agtttcgggc gtttctttat
    29281 tgaaggatta cgtacagaca gtctttactt tttaggtgtg atccgtattt ctcaattatt
    29341 atctgctgtg ttatttgtcg gtacattgat tctatttatt tggcgcagaa aaaaaggaaa
    29401 tttaccggat tatgaccgct cattaggaaa aaatcaacaa attatctgat ttttagagcg
    29461 ttaaaaatga aaaaaatttc aggagtggta aaatgaatta tagagtagcg gttttaggac
    29521 caggctcttg gggtactgct ttggcacaag tcctcgccga aaacggacat gacgttcgga
    29581 tttggggaca tcatgcttca caagtagaag aaatcaacac ccaacacacg aatcaacgtt
    29641 atttaccaga tcattatcta cccgattcca tccaagcatt caccaatatg gaagatgcag
    29701 taaaagacgc agatgcggtg ctattcgtca ttccaacgaa agcaatacgt tctgttgcaa
    29761 aagaactagt gcctaaattg aatacaaagc cagtgatcat ccatgcaagt aaaggattag
    29821 aacagggaac acataaaagg atctctgaca tcttgttaga ggaaatccca gcagaaaaaa
    29881 gaaaagctgt tgttgttctt tctggaccaa gccatgcaga agaagtagca aaacatgaca
    29941 tcactacgat cacagctgca agtacagatg caaaagcagc aaaatatgta caagagctgt
    30001 tcatgaataa ttattttcgg atctatacca atcctgatgt gatcggtgta gagatggggg
    30061 cagctttgaa aaatattatt gctattggtg ctggcgctat tcacggctta gggtttggcg
    30121 ataatgcaaa ggcagcaatc atgacgcgcg gattagctga gatcagccgt ttaggtgtag
    30181 caatgggtgc caacccgctg acctttatcg gattaagcgg aataggcgat ctagtcgtta
    30241 cttgcacaag tgttcattct aggaactggc gcgcaggcaa cttgttagga caagggcagc
    30301 ccctagaaga ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat actacccaag
    30361 cagcgatgga actagcccaa atgatggaaa tcgaaatgcc catcactcaa acaatctatg
    30421 aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg ttacgtgatg
    30481 gaaaaaaaga aaatgaattc tcattaacaa ttttttgaag gagcgaatgt atatgaaagt
    30541 taaaaaagca gttattccag cagcaggatt aggaacaaga tttttaccag caacaaaagc
    30601 tatggcaaag gaaatgttgc caattgttga taaaccaaca atccagttca tcgttgaaga
    30661 agcgttgaaa tcaggtatcg aagacatctt gatcgttaca ggaaaagcta aacgaccaat
    30721 cgaagatcac tttgattcaa atattgaatt agaaacaaac ctaagtgaaa aaggaaagac
    30781 cgaattattg aaattagtag aagaaacgac agatgtcaac ttgcatttca tccgtcaatc
    30841 tcatccaaag ggtttaggac atgccgttct tcaagcaaga gcctttattg gaaatgaacc
    30901 atttgtcgtt atgctaggcg atgatataat ggaagacgaa gtaccattga cacagcaatt
    30961 gatcgatgac tatgagcgta cacacgcttc tacgattgcc gttatgcaag ttcctcacga
    31021 tgaaacttca aaatacggaa tcatcaaccc aggggaagta ctggaagatg ggttatacaa
    31081 tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt tagctattat
    31141 tgggcgttac ttattgactc cagaaatctt tgatgtatta tcagaacaac aaccaggtgc
    31201 tggaaatgag atccaactaa ctgacgcaat cgatacgttg aataaaacac aacgcgtatt
    31261 tgcccgtaaa ttcacaggta aacgttatga cgttggtgac aaatttggtt ttatgaaaac
    31321 aagtattgaa tatggtctga cacatccaga agtaggggca cctttgcgtg aatacatcat
    31381 gtctttaggt aaaacattag aagcagaagg aagcaagatc aatcataatc caaaaggaat
    31441 aacagaaatc aaagaagaaa agaaaaaata atcttttttt ctaacgagct tcagctgcta
    31501 attgaataaa aagtagttga agctcgtttt tatttgttta aaaaacacga aaatgctatc
    31561 cttatagaaa agagagacaa gaagggagtt tgatcatgtc aggaggagaa attgcaggat
    31621 tgatcgcagc aatcgccttt gcgatacttg tcgttttcat cgttcgcgta ttgttgcaag
    31681 tttccaaaac tttagaaaaa gtagaccaga ctgtcgcaga agcaaatacg acaattgaga
    31741 tcgtcacaca agacgtagat cttttatcta gacaagtaga aggcttgcta gtcaaaagta
    31801 atgagctgct cttggatatc aatggaaaag tcgcaacgat cgatccattg ttcacagcag
    31861 ttgctgattt aagtgaaagt gtatcggaac ttaatcattc tagtaaaaac atcgtgacga
    31921 aagtcggcgg aatcggaaaa acgacagcta aagcgactgt tgccggaaaa gtcggtggaa
    31981 cagcgatgaa attttttaaa aacaaaaaac atactgaaac gacaggagga aaataagaat
    32041 ggctaaaaaa ggtggatttt tattaggtgc agtagttgga ggtacagccg cagcaatcgc
    32101 cgcattattg ttagcaccca aatctggtaa agaacttcgg gatgaattgg ctgctcaagc
    32161 agatgatttg atggacatgg cttcagatta cacagatatg gcaaaagaaa aatcatctga
    32221 gttaacaggt attgcaaaag aaaaagcgca aggtctttct caacaagcag gggatctagc
    32281 aggcaatgtt aagcagaagg taagtgaatc agcagaggaa gcaggaagtg aatcagatga
    32341 tatcttgtta aacctcaaag aacaatcagc tgatttatct aatcgtttca aaaaaacagc
    32401 agaagatctg aaaaagccag cagacgaatt aggtagtatt gctaaagata cttcagaaga
    32461 tatcatgatc gatgtaaaag atgctgcaga taaagcagga gagacggtca atgaaggaat
    32521 gtcagaagca aaaccagttg cagatgacac aaaagaaatg ttgaaaatgg caaaagaaga
    32581 tgcaaaagaa gctaaaaaag aaatgaaaga tacaatgaca gattcgacta ccaatcacta
    32641 agattactca tttctagaat aaaaaaggtg agagcggacc agctctcacc ttttttattt
    32701 tcattactct aaaacaagta attcttttgg tgtagtcgtg aatggcacac aaccatcact
    32761 tgtcacatac acacaatctt cgatacgtac ccctactttt tcaggaatat agattcctgg
    32821 ttcaagagag aagcacatcc cttcttcaat cactaagtcg tttccttcta caagagaagg
    32881 gaattcgtgg acggttgttc cgattccgtg acctaaacgg tgattgaaat attcgccgta
    32941 gccatagctg ttgatcacgt ttcgtgcgat ttgatcaagt tcacctgctg tcacacctgg
    33001 gcgaactgcc tcttgtgcgg caagctgtgc ttcaagagtg atgttataga ttttttcttg
    33061 gaactcagtt ggtttttgat aagcaactgt acgagtagca tcactgcaat agccattcca
    33121 aataacgcca agatcgaata acactagttc atttggtgaa accttcgtgc ttcctggtgt
    33181 accatgaggg cttgcagcat ttgctcctgc aaggactaat gtgtcgaaag acatttgtga
    33241 aacaccttgt ttttttaatt ggtattcgat ttctgcgata attgcttgtt cagttacgcc
    33301 ttcttttact gttttgaagc caatttcaaa ggcaacatcg gcccatttac cagcttcgat
    33361 cagtttgtct ttttcttcta ccgttttgat cagttgcaga cgctggataa tagaagttag
    33421 atcggtgtaa gaatctgtac ctgtcaagat atcagagata gcatcgaagc gatcgacagt
    33481 cagagcattc ttttcaatac ctacattacc aggttgccca tagcgtgcgc gaagctcttt
    33541 tccgatgatc tcccaaggat tttcgctgtc taagtagcca cgtacatcgt acgcccagct
    33601 gctgttttga gcatcttcaa cttccagagc aggagtgaaa aggaaaggat cttgttccag
    33661 agggataaaa agagctagta cacgctcgtg aggatcactt tcatatccgg aaaagtaagc
    33721 gatgtggcca gggtcgctga tataagccaa ttctacttgg tgttgtgcca tccattgacg
    33781 tagttcattg atttttacgt tattcatttg gcaacctact ttctaaatta attttgtatt
    33841 caataattgt accactgatt gattaaaaga ccaacgttct taaaactgaa aaaagtaaaa
    33901 attttcatga aaacagagat aaaacggttg caattgttga gattatttga tatcctattt
    33961 tagaaatgta agacgtattt tcagaaaaca tataaaatat gaataggaga acacaaatgg
    34021 aaaagcaaac aatcactatc tatgatgtag cgagagaggc aaatgtttcg atggctactg
    34081 tttcacgtgt cgtaaatggc aatccaaacg tcaaaccagc aacaagaaaa aaagtcttgg
    34141 aagttatcga tcgcttggat tatcgtccaa atgcagtcgc acgtggcttg gcaagtaaga
    34201 aaacaacaac agttggggta atcattcctg atgtcagcaa tatgttcttt gcctctttag
    34261 cacgtggtat cgacgatgtg gcaacaatgt acaaatacaa tatcatctta gcaaactcag
    34321 atggcaacga tcaaaaagaa gtcaatgtat tgaataattt attggctaag caagtggacg
    34381 gcgtgatctt tatgggtcac catatcacag acgaaatccg tggagaattt tcacgttcaa
    34441 aaacgcctgt tgtactagct ggttccattg atccagatga acaagtaggt agcgtaaaca
    34501 ttgattatgt gtcagcaaca aaagatgcag taaataaatt agcaaaaagc ggcaataaaa
    34561 aaatcgcttt tgtcagcggt gcgctgattg atcctatcaa tggtcaaaac cgtctaaaag
    34621 gctataaaga agcattaagc gaaaacaatc taccatatag cgaaggattg atttttgagg
    34681 cacaatacaa ctttaaagat ggtctgtctc tagttgaccg gatccataat agcggtgcaa
    34741 cagctgtcta tgtttcagac gatgaattag ccattggtat cttggatggt ttattagacc
    34801 gcggcgtaaa agtaccagaa gaattcgaaa tcatcacaag caacaattct ttactgacag
    34861 aagtagctcg tcctcgtttg acaagtatca cacaaccatt atacgattta ggagcggtat
    34921 ccatgcgcct attgacgaaa atgatgaata aagaagaagt cgaagaaaaa acgattatct
    34981 tgccttatgg aatcgaagaa aaaggatcaa ctaaataata aaagatcaat catttaaagg
    35041 ttgttccaaa agtggtcaac ttctggaaca acgtttatac gagaaagggg gttatgacaa
    35101 aattttgtca taaccccttt ttttttgtga ctttaaaaaa agagaaaatg gcggaaacaa
    35161 accttacgtc catcgtatac gacacaatct gtgcgtagat gctacatttt gttcactact
    35221 atcaaaatta ctttaaaatg aaaacgtgat aaaaaagaaa gtagaaaagt agataagtaa
    35281 ctctaaaaaa tccaaaaaaa tccaggctcg attgcgtact tatcttaaag gagaaacaat
    35341 ccatcatata taacccgaaa ggagacttct atttgcctaa gaaaagacga aagaccatag
    35401 tcatcatttt gaccattctt actataggaa tcggagggaa attttacatg gacaaaagag
    35461 aaatgcaaag acagaaagat ttactcgcag tagaaaaaca gtccgtcaaa gttctgaaaa
    35521 atacctttgc ggatatcaag gaagtgaaga ttgaaaaatc tgctcgaaat gaaatgacgg
    35581 gttcataccg gatagttatt ctaatgacta ataaacaaga tcaatcgatt tatttttctt
    35641 atagtttttg gaaagaaaga aatgagatag gaagttacgg aatagttgat gaaaaaaagc
    35701 aaaaagaagg aaatacgctc aataaagtta aagtaactta ctctaatgga aatgaggaaa
    35761 gtatttaatg attactgaca aacaatatag tgaattaagt gatgctgttt actggctaga
    35821 tcctaaacat agagactatg tacctgaaat gcaagaaaat ttaagtttta aaattaacgg
    35881 cactatgtat aaaatcctaa aaatcaaaaa ctccttcgac ggcatgcagg cgatggctgt
    35941 agcgccaatc gtccatagca aattggagaa aaatttcaaa aataaaaaaa ttcctgcaaa
    36001 ctttcgcgta cttaaataat gaaggtgtca atacttacat aaaaagaaag gaggcacgtt
    36061 cttgtcaagg aaaataagaa aactcagctt agccatttta ttaatcgtca tattagggat
    36121 cggagggaaa gtctatatgg acaaaagaga agcacaaagg cagaaagatt tactcgcagt
    36181 agaaaaacag tcagtcaaag ttctgaaaaa tacctttgcg gatatcaggg aagtgaaggt
    36241 agaagaattt aaaaaaaacc ctgttaccgg aagttatggt gcacttgtga caatgacaaa
    36301 taatgaaggg aaatctgttt attttgatta tagtttttgg aaggagagaa atgaacttgg
    36361 tggttttggc attgagaaca gagcggtaca aaagacagga gtcactataa aaagagtaaa
    36421 agtaattttt tctaacggac aggaggagtt agtatgacaa taaacgataa gcattacaat
    36481 gatattagtg agaaggttta ttggctagat cccaaatatc cccgctacaa tgagggatac
    36541 aaaaagaact ctgttaaaga atttgctggt atggaatttc aaatcctaca aatcaaagac
    36601 tcattggacg gcatgcaggc gatgtctgta gcgccaatca agaacggtaa agtggatctt
    36661 tctcaagtag tgattgcttt tgcaggaacg aacttaagtg attggaaaga tttggaaacg
    36721 gatgcaagaa gtatcatgaa tctctacggg aatagaccag gagcaagttt ttctgaaaaa
    36781 gcaacaacaa acaagatttt atccgttagt ggattgaaaa cagcggggca aatcaattca
    36841 gcgattgcat ttgctaatga agtaaaaaag gaatatggag aagctgctat ttctactacc
    36901 ggccattcat taggtggatt cttagcatta tatgtagcag cagagaaaca atggaaaaat
    36961 gtcggcttca acgggccgga cccgtatgat ctcttatcac cagaagcaaa aaaatgggtg
    37021 gaagaaaatc ccggtatgct caccaattac cgaaatcacg ccgattttat cggtaatttt
    37081 ggtggtaatg gcacaggtgc tgaagttcgg atcagtatgg aaacagcatt cttgaatctg
    37141 cttgcttccc acagtttaac ctcttggaag tttgatgaaa atggaaagct gattattcct
    37201 gaaaatgaaa acaatgttaa agctcgaaaa caacagaaag aaaaccaagc gacccagttg
    37261 ttttcctatc agttgcaaga actgaacaac atacgtaaaa aactacgatt aagtggcggc
    37321 cagctgacag ttaatgaaca aatttactta gatgacacac aagctaggtt agtagtggaa
    37381 aagttatcgg caaaaatgaa aagcgctatg gaagaagtta tccgcatcaa tcaaagagga
    37441 atttttgagc tagagaaaaa atggcaagaa ggcttgcggc aagcacgtgt tgtagcccct
    37501 tcgttgactg aacaggaaat catagaagca ttagctagtg tgggcgccac aaaagaaaac
    37561 ttagtcgatt ctgcgaaaga agtctatctg ctacgaatca ataaggcaag acgtatgggg
    37621 gaggcctttg atacattggc gaaggagatc caagacaaaa tcaatgaaat cgtggcacga
    37681 gatcgagaat tagcacagca attcaattaa aggagaagta gatgaaacta gaagaattgg
    37741 aaagagatta tcgaagtgaa cgaagacaat tagaagctca agaagatgaa ttgatccact
    37801 ttaagaatca aggattacga ttgatggagc aagaggagca gcgactgcat catgtattaa
    37861 agaacttagc tttagataat gagcctctgt ttcaagcaag acgaagttat tcgaattatc
    37921 aggagcatta tcaacaagcc ctacaagtag aacgacgaaa actctatcag cagcaagatg
    37981 aactagaacg ttcctatcgt caaaaaatca gacaaataac tatggagaaa tcatcttatg
    38041 aatgaatttt taatgcaatt actaaaagaa ttagagaaaa atctacatcg atcaaatgta
    38101 cgttcagttg gtatggggat atctcagcaa atttgggcag aaaaacgatt gacagatcaa
    38161 aactaccaaa tgactgcaga agaagctgct caagaatttt ctaaccgaca tgtacacttg
    38221 aaaggatcag taaaagggca atttgcagaa aaactaactg aattattcca aagacaagaa
    38281 aagaaattaa aacagtttcg ttagtaaagt tatatagaaa aattttgtga aaatggtt
//
LOCUS       DAHUVV010000018        36665 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.018, whole genome shotgun sequence.
ACCESSION   DAHUVV010000018 DAHUVV010000000
VERSION     DAHUVV010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.018"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            205..1053
                     /locus_tag="NK767_000930"
     CDS             205..1053
                     /locus_tag="NK767_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCH6693941.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPRPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            1198..2139
                     /locus_tag="NK767_000931"
     CDS             1198..2139
                     /locus_tag="NK767_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HCH6693942.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLKKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFTHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            2129..4300
                     /locus_tag="NK767_000932"
                     /pseudo
     CDS             2129..4300
                     /locus_tag="NK767_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(4412..6583)
                     /gene="copB"
                     /locus_tag="NK767_000933"
     CDS             complement(4412..6583)
                     /gene="copB"
                     /locus_tag="NK767_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HCH6693943.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(6603..8789)
                     /locus_tag="NK767_000934"
     CDS             complement(6603..8789)
                     /locus_tag="NK767_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCH6693944.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(8789..8998)
                     /gene="copZ"
                     /locus_tag="NK767_000935"
     CDS             complement(8789..8998)
                     /gene="copZ"
                     /locus_tag="NK767_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCH6693945.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(9011..9451)
                     /locus_tag="NK767_000936"
     CDS             complement(9011..9451)
                     /locus_tag="NK767_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HCH6693946.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(9525..10064)
                     /locus_tag="NK767_000937"
     CDS             complement(9525..10064)
                     /locus_tag="NK767_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCH6693947.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(10679..12145)
                     /locus_tag="NK767_000938"
     CDS             complement(10679..12145)
                     /locus_tag="NK767_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6693948.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(12456..14537)
                     /gene="cadA"
                     /locus_tag="NK767_000939"
     CDS             complement(12456..14537)
                     /gene="cadA"
                     /locus_tag="NK767_000939"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCH6693949.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(15062..15421)
                     /locus_tag="NK767_000940"
     CDS             complement(15062..15421)
                     /locus_tag="NK767_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCH6693950.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(15451..15792)
                     /locus_tag="NK767_000941"
     CDS             complement(15451..15792)
                     /locus_tag="NK767_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693951.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(15789..16463)
                     /locus_tag="NK767_000942"
     CDS             complement(15789..16463)
                     /locus_tag="NK767_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCH6693952.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKSLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(17515..18813)
                     /gene="asnS"
                     /locus_tag="NK767_000943"
     CDS             complement(17515..18813)
                     /gene="asnS"
                     /locus_tag="NK767_000943"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCH6693953.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(18853..20043)
                     /locus_tag="NK767_000944"
     CDS             complement(18853..20043)
                     /locus_tag="NK767_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCH6693954.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(20064..20591)
                     /locus_tag="NK767_000945"
     CDS             complement(20064..20591)
                     /locus_tag="NK767_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HCH6693955.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGYESGDEVKTIKGI"
     gene            complement(20638..23427)
                     /locus_tag="NK767_000946"
     CDS             complement(20638..23427)
                     /locus_tag="NK767_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCH6693956.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKATLKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(23574..23774)
                     /locus_tag="NK767_000947"
     CDS             complement(23574..23774)
                     /locus_tag="NK767_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCH6693957.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(24226..24546)
                     /locus_tag="NK767_000948"
     CDS             complement(24226..24546)
                     /locus_tag="NK767_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693958.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHILHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(25049..26167)
                     /locus_tag="NK767_000949"
     CDS             complement(25049..26167)
                     /locus_tag="NK767_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693959.1"
                     /translation="MAKKDKELLSQLISEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FSKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            26640..27947
                     /locus_tag="NK767_000950"
     CDS             26640..27947
                     /locus_tag="NK767_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCH6693960.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKVSGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            28065..29264
                     /locus_tag="NK767_000951"
     CDS             28065..29264
                     /locus_tag="NK767_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCH6693961.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRVIFSSNNYHIF
                     RAGLYACQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(29291..30559)
                     /locus_tag="NK767_000952"
     CDS             complement(29291..30559)
                     /locus_tag="NK767_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HCH6693962.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DTRLSHPWLLYLLPVSGALFAYLYVYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(30722..31369)
                     /locus_tag="NK767_000953"
     CDS             complement(30722..31369)
                     /locus_tag="NK767_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HCH6693963.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELLEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(31777..32280)
                     /locus_tag="NK767_000954"
     CDS             complement(31777..32280)
                     /locus_tag="NK767_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCH6693964.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(32403..33167)
                     /locus_tag="NK767_000955"
     CDS             complement(32403..33167)
                     /locus_tag="NK767_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCH6693965.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            33274..33549
                     /locus_tag="NK767_000956"
     CDS             33274..33549
                     /locus_tag="NK767_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCH6693966.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            34145..35095
                     /gene="yidC"
                     /locus_tag="NK767_000957"
     CDS             34145..35095
                     /gene="yidC"
                     /locus_tag="NK767_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCH6693967.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(35274..36248)
                     /gene="lacD"
                     /locus_tag="NK767_000958"
     CDS             complement(35274..36248)
                     /gene="lacD"
                     /locus_tag="NK767_000958"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCH6693968.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDTTIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGILCGR
                     ATWAKGVAPFVTEGEKKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(36245..36418)
                     /locus_tag="NK767_000959"
                     /pseudo
     CDS             complement(36245..36418)
                     /locus_tag="NK767_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
BASE COUNT        10524 a         7735 c         6505 g        11901 t
ORIGIN      
        1 aaaacgaatg tactttaaaa aaacataata agatcacttt gcttgtagga tgtattccct
       61 tccattacaa tgaaataaat agtgatttta tgagaaggtg atgtgaaaat ggaagaaaaa
      121 atagaagaat ccaaacaaaa aatcgatcag ttgatataag aattagaaaa agcggtcatc
      181 ggcaaacgtt caactatcca attgatgatc gtggccctat tagcaggtgg tcatgtctta
      241 ttcgaagata ttcccggtgt aggtaaaacg ttactggtca aagcactagc aaaagctgta
      301 cagggaacct tcagcagagt ccaatgtact cctgatctgc tgcctagtga tattcttggt
      361 ttttccgtat acaattcaca atcaaaagag tttgaatttc gtccgagacc gatctttacc
      421 acgatcttgt tggcagatga gatcaatcgt acgactcctc gtactcagtc agcactttta
      481 gaagcaatgg ccgagaatca tgcaacgatc gacaacaata cataccctct agacaatcat
      541 ttttttgtct tagctacgca aaatccaatc gagtacgaag gaacatatcc tttgccggaa
      601 gcacagctcg atcgtttttt attccgacta caaatcggtt atccttcttt tgatgacgaa
      661 cttttgctac ttttggataa attcgaaaaa gtgttggata acttaaatga agtattgaac
      721 agttatgaaa tagaagaaat aaaaacaaat gtggcgaatg tctttgtcag tcccgaagtg
      781 gcatcttatg ccttgcagtt agtgtcagca acaagaagtc atgctgccat ccagcttggc
      841 atcagtccac gaggaagcct ttcctttatt caggcagcaa aagcttatgc tttaatccat
      901 gggagaaatt acgtcacgcc caaagatttg caggatttag ttccttacgt gttttcccat
      961 cgcctgattt tttacgatcg ttcattgaag gaagacgaaa agaaacaaat cattcatact
     1021 cttgttgcac aaattcctat tcctgtgagg tagattgagg tagacaatgg aaaaaatcaa
     1081 agattatttc attaattttg tgttatttct ggtatatttc atcattgtgc tttttgctct
     1141 tgtattcaac aattctttag gctggttctt gttggccttt tttactcttt atgtatcatg
     1201 gagcttgatt tcattaagca gctttctaaa gaaactttcg gttacttgta cattgcagcc
     1261 actcgtttat cgcaaaaaaa gtatgcaggt tattttttcc atagcaaaaa agagaacttt
     1321 ttctttactt ctccctcgtt tacaaatcgg cttcccagca atctttgcag aaaaaaacaa
     1381 gagattttga ttcttacaaa taaaccgcaa gaagtccaat tgctttggca gccaaaagaa
     1441 agaggatgcc atgaaacatt aactgtcatt tttacagcct atgatagtct aggacttttc
     1501 agaaaaagtg caaaaagaga gatctctttt cctgtgactg ttctgcctgc tttttgtaag
     1561 attcaagcag aaaaattaca gagagttctg gaaaaaaaac agcagctgaa cgcgtaccaa
     1621 agaggtcttg attttcggga acatcgcgct tatcgtccag gtgattcttt caaccgaatc
     1681 gattggaaat tgtctgctca tactcaagag tggttatacc gtgaatacga ttatcaagaa
     1741 gaagaatgtt ctcctctttt ttgcttttgg ggttcgccca gtccaaaatt tgaacctctg
     1801 ctagacttgt atttttcttt atggcatttg aaaaaagaaa agcagccgga acttctgata
     1861 ttaggcaacc gcgcctttca tggcaaagac cccggtcata cctattttgc ttctttggaa
     1921 gctggcgatg agaaagcttt tttcacacac ttgaaaaaat atgcaaaaaa acaaatcgtc
     1981 ctctttgttc ctgagaattc tcctgaagtc tcagaagcta ttgaaacatt gtcaaaagat
     2041 cgaacggttt atcttgttta tttcgtagaa aaacaactca tggtccaaga aaaagacaag
     2101 atatgttcat tgccaggagg tgaatggatt gacgattaaa cgggaaaaat ggctgcgtgg
     2161 cgtttactcc tttgtgatac taagtgtagg tgctccgata tttgtagtag ctaatcaatt
     2221 gcaaaacagc taccttctta tcctaactat tggtgtccta tgccttattg tctgctgtat
     2281 tcctaaaaaa tgggtacgtt tgctgctttc tttgccagta atgataggtt gtttgttttt
     2341 atattttcct tcgaagacat tttccatatt atggatctta cagttttttt cgtcagttac
     2401 agaggaattc ttccatctat cccaaggtaa actttattat ttgccagaaa agacagcttt
     2461 ttttctcata atgttgctga tctatttatt tattacattg accgtggatt acgactattg
     2521 gtacgcaccc tttctttctt tgatggctta cttcatgatg cttaccgttt ttcggaaaaa
     2581 agatttactg tacgaaatga ttgcggtagt aactgttttg ttcgtgtatc tggcagcaag
     2641 gcaatttttc tccatccggt tgttgtcggg aaacactcga ttccagtcat tgactacagt
     2701 tctgcttatt tgtttcttaa cttttagcgc tatcttgcct ttatatcttc cgaaagtaca
     2761 ccaatctctc gaggaaacga gtgaaccgat ccgagaatat cttaatgatg aagggcttta
     2821 tgacacactc catgagtatc aattaaccgg aagtgcaaga acaggcttca gtgagaatga
     2881 tgaacagctt ggtggacctt tatatgataa tggcgaagtt gtttttacag cgaaccaaga
     2941 aggaaatggc tattggaaga tcgaaaataa aaatcattac accggttctg gatggataga
     3001 agatcaggac gcacccatcc aaccaatcca agaattccct gttgacatca cgagctacca
     3061 aaccgttata gaagacaaag aaaacattac attgacccta aaaaaacaag ttttcttacc
     3121 ttatccttat ggaaatacta ccttgactga tttttcatta gctagtggac aaacgcttta
     3181 ctatttgcct gaaagccagc gattcattcc tgtcccgaat gaatcgacac gttctgcaac
     3241 tttacaaaca gaaaggttag tttttacgag agaagaatta aaaatcagtc agttaaaagc
     3301 tgatgattta gaaaatcagc agacaaatct tcaattgcca aaaagtttgc ctaaacgtat
     3361 tgaggacttg gcggtcgaat tgaccaaaaa tgaagcaact ttaattcgta aagtcgaagc
     3421 tgtctaacag tatttaaaaa caagcggaaa tttccgctat tccaaaacgg ataccgggca
     3481 tactccaaaa gaacacgatt atgtggatta tttcctcttt gattcaaaag tcggctattg
     3541 tgacaacttt tcaacttcta tggtcgtcct tcttcgaaca cttggtatcc cagctcgctg
     3601 gacaaaaggt tttacaaatg ggctcagaac aggcacagat aatcaaggga atgaggaata
     3661 cagcattctc aatagtcatg ctcattcttg gccagaggta tactttcctg gatttggctg
     3721 gcttccattt gaaccaaccc cttcatttgc caatccaatg ggttcacaac cagttgctga
     3781 aacaaccgaa accgtccagc ctgctatcga acaaccaaat gaaagcgaaa atatggcaga
     3841 aactatttcc agtgagagat ctgaggtcat tccatcagaa gaaacaacac tcacaccagc
     3901 ttataaaatc gtcttattca gccttggagt gtgtttatcc gtcttgttgt tctttctatt
     3961 gttattcaga aaatctctgc gctggacaag ttatcttatc tatcttcatt tttttgcttc
     4021 tactaatacg attgcatatc aaaaacttct taaccctttg gaaacattgg taccgcgcaa
     4081 acccgcagaa tctttgcagg aatatagcct gcgaatagaa caagaattca agctagatct
     4141 gcgtttcatt cgattaacta ctcaatatga agcagaaata tatggacgac aagcagatcc
     4201 ggtaaaattc gataaaaaaa cctatttaga aataacgcat ataataagaa aaatggtata
     4261 caaaaaaatt ctgacaaaag ggagccgctt caaaaaataa gataaaattt ctggagaatc
     4321 tacttctgga atgccgagaa ccagctgatt gagtatcgat tactcgaaaa aaaggttgtg
     4381 acacgttttt tgtcacaacc ttttttcatt attatctgat tttcaatgtc aaagcattta
     4441 gagctacaac taccgtactt aacgacatca agacagcacc aactgctgga cttaatatga
     4501 taccaattgg cgcaagaatt ccggctgcta gcggaattgc aagaatattg taacctgccc
     4561 cccaccagag gttctggatc attttctttc gcgtttgctt ggctagatca agaaaatgca
     4621 aaatatcttt tggatcgctg tttgtcaata cgacatccgc agaatcgatt gcgatatctg
     4681 tccctgcacc gattgccatc ccgattgaag cacgggctaa acttggcgca tcattgatcc
     4741 catcacccac catgattact tttttccctt ggtcaaggta tttctggaca acagcttctt
     4801 tatcatctgg taataaaccg ccataatatt cttcgatccc taaataatct gctactgctt
     4861 gggcagcgtt tttattgtca ccagtaagca tgacaggagt gatttttctt tctttcaatg
     4921 ttcggataaa ttcttttgct tctgttttta ctacgtcacc taaagcaacg attgcagcta
     4981 gatgattttc taaaatcagg aagctgatgg tatttccttg ttcttggtaa ggtttcaata
     5041 atgcttgttc tacgtttaat ccgagacgct caacttcttt ttcattaact attttcactt
     5101 gttgtttttt gactgttgcc tctagcccga ctcctgaaag attttttagg ttttcagctt
     5161 gataaggctt gatttcatgt tctttcaaat aattcataat gccaacagcc aatggatgat
     5221 tggcattcgc ttccaaggct cctagatatt gaaggatttc atttttattg aacgcatcat
     5281 caagtacttc gacacccgtt acagtaaatt ttccttctgt tagtgttcct gttttgtcca
     5341 gcatgatcac atctagatca ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc
     5401 cgtttttagc tgctattgat gtggaacgag caacaactaa tgggatagct aaacctagag
     5461 catgaggaca ggcgatgata aagactgtta ccatacgttc taatgcatta ggaagatctg
     5521 tcaagaacaa ccaggcgatg aatgccagga tacccacaat taatgcgaca taaaacagcc
     5581 attttgccac tttgtcagat aatgactcta gcttggattt ttctccttgt gcttttcgca
     5641 ccatttccat aaccttcgct agataaccat tttcaccagt tccagttact tcgatttcga
     5701 tcgttccatc cccattgata gaaccgccaa tcactttgtc attggcttgt tttttgattc
     5761 ctcttgattc gccagtcaca gctgattcgt caacaattgt ttcacctttc aagattacac
     5821 catctgttgg cattttatca ccagaccgga cgatcaaacg atcaccttcg tttacttctt
     5881 tcaatgaaac tgtctcttct tttccatctt tcgtcaagcg tttcactgat tctggcagta
     5941 attctgctag tttttgtaaa gcattgctgg cattcgacac agcattcatt tcaatccagt
     6001 gacctagtag cataataacg atcaaagtag ccaattccca gaagaaatcc atgacgtgtg
     6061 tatgtggatt gagcagattt gcgataaacg aatagacact ataaatatat gcaactgtaa
     6121 tccccatcgc gatcagtgtc atcatagcag gacttttttg tttcaattcc atcttagctc
     6181 cacttaaaaa tggctgaccg ccatagataa atagtatagt tgctaagatc aatacgagcc
     6241 aatcagaacc aggaaacgtt acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa
     6301 gaatgattgg gatagctagg ataagcgata gccaaaattt ttgtttgaaa ttccccatgt
     6361 gcatggaatg atccatgccg ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt
     6421 tgctgtttgc tatatctcca tggttatgtc ccatgtgcat ggaatgatcc atctgactat
     6481 ggtcatgttt catctccatc tgtttttcat gagattctgt actttctttg tttactactt
     6541 cttttttatg ctcatgtttt ttctccatat cgttcccatt catttttact ccatcccttc
     6601 tcttactttt tcatgtgtcg atttaagctc agggagttca acaatacact gacggaacta
     6661 aacgccatcg ctcctcctgc aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata
     6721 ccaatcgtat tatagacaaa agcccagaaa agattttgtt tgatttttct caatgtcaac
     6781 ctagacaact cgattgtttg tgcaatggat gccaaactgc tattcatcag cgtcacatcg
     6841 gctgtttcca tcgcaatgtc cgttccactt cccattgcca taccgacatc agctaatgct
     6901 aaggccggtg catcattgat tccatcgcct gccataccga cttttttgcc atctttctga
     6961 agtttttcta cgtaggctgc tttatcctct ggaagtactt cagcaaagat atgctttgga
     7021 tcaataccaa cttgtttacc aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata
     7081 aaaacatcta atcctttatt ctggagctgc ttgattgctt gtttgacttc tagtttgatt
     7141 tgatcagcca cggcaatcaa cccgatcact tgcttttcat cagctaaaaa catgactgtt
     7201 tttccctgct gttctaattc taacgcgtgt tcttggtatt catcaaatga taggttcaat
     7261 tcaattaatc gtttacgtgt acccgcaaaa tagcgtactc cgttaatcgt accgctgatt
     7321 ccagcacctg ggtgggctgt aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt
     7381 ccgtattcca cgattgcttt acctagtgga tgttcagaag cttgttctaa agtataaaaa
     7441 atagatagga cttctttaga accgactaga tctgtaactt tgggctttcc ttctgtaatc
     7501 gttccagttt tatccaaaac aatgctgtct aagtgagcag ctgcttctaa tgcttctccg
     7561 cctttaatta aaattccatt tcgtgcacca acacccgttc ctaccataat agctgttgga
     7621 gtcgctaaac ctaatgcaca tggacacgca atgactaata cagagacact atggatcaat
     7681 gcgagttgcc aatcaccagt gatcaaacct gtagctatga gcgtgataaa cgcgatgact
     7741 aatacgatcg gaacaaaaat ccctgagatc cgatctgcga ttttttggat tggggctttg
     7801 ctcccttgag catcttccac catttgaatg atttgcgcta atactgtctg cttgccgact
     7861 tgtgtaactt ccatgtgaag tagaccattc gtattgattg tcccgccaaa gagcgtgtca
     7921 tccggattct tttctacagg aagattttca ccagtaagca tactttcatc tatcgcactg
     7981 cttcctgaaa taattcgacc atctgctgga atctgctcac caggacggat gactagttga
     8041 tcaccaacga ctacttcttc aatcgctaac gtttcttctt tcccgtttct tatcacttgg
     8101 gcggtttttg tttgaagaga catcaattgt ttgattgcat tacctgtttt cgtttttgct
     8161 gtatgttcca aatatttgcc gagcaaaatc agcgtgatga tcatactgct gctttcaaaa
     8221 taaagatctt gggggtgtcc acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc
     8281 gatgttccaa ttgctaccaa tacatccata tttggtgctt tcgtcttgat tgcatggtac
     8341 gcacctttat aaaaacgagc accgacataa aattggacag gagcagacag cactaactgg
     8401 acgattggaa aatgaaaaaa gtgaacaatc gccgcatggc ttcctaacat catagcaatc
     8461 attgacaaca tcaacggcaa agtcaaaacc gtactcaata taagatcgaa tagcattttt
     8521 ttcaaatacg cttgcttttc ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt
     8581 gcaccatagc cgatgttttc tacgctttgg attaattttt cagtggtcgt cccttcatat
     8641 ttgacacttg ctttttcagt ggccaagttg actgtggcat tgaccacacc aggttgtccg
     8701 ttcagctctt tttcgatcct agcagaacaa tttgcacaag tcatacctgt aatgacgaac
     8761 gtatccattt tggtttctgc tgttgccatc aaatcacctc tgctttataa cccaatccat
     8821 tgattgcttg acaaatcttt tcggattgaa cttttgtttc atcaaacttg accgtaccgt
     8881 ttgctttttt caaatttact ttcacttttt gcacgccgtc aagtgcgctt actgtttctt
     8941 ctactcttgc tgcacaatga ttgcagctca taccttcgat tgaaaattgt tgtttcattt
     9001 tttcttcctc ctattgatgt tgtttacatt cacattgacc tgggatacag ttacattcga
     9061 tcgtatctac tggttgcttt tgttccagtt gtttcaaaat ctcattgata tcttcttgag
     9121 tcaaagtggc ttcttctact agatccgcaa tcgtctctcc aatccttttt gcacagatat
     9181 gggaaaaaag attttccgtc gcactgcgaa ctgtttccat ctccgagaca gctggatgat
     9241 aaatgaattt ttttccttct tgttctgtcc agatcacttc tttttttacc aaacgaccca
     9301 gcaaagtttt gatcgttgct ggtttccaat ccatcgaatc agctaagatc tgcgtgattt
     9361 cttgggctgt cgtctgtcca agtgtccaaa taacacgcat gatctcccac tctgaatcac
     9421 tgatctttac tggcgttttg actttttcca tttatttcac acctttcgtt tacaaatgta
     9481 atcattatct tatgaataca gattacactt gtaaacaaaa actgtcaatt atttttgctc
     9541 tctattttca atttagttaa aaattcttgt ttctctcgtc ttgttttaag gatgatcagt
     9601 ttatttcttg cattagctac ttggatcagc tgctgtattt tcggttcatt gttttcttca
     9661 aaatgccaga taaaagataa aaattctaag tattccctgt ttagtttctc cgaaaatgat
     9721 tcaggcatat ctggccgaga ttttttgttt atctttcttt ttagagaacg ccaaactact
     9781 cggtagatgg cgagataacg cggcacttta agccaaatga tcttatctgc ttctttaatc
     9841 ctttcctcta atgtagaagt ataatttcct tcaacgatcc atgacggatt agataccata
     9901 aacaattgct gttcttgcaa aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc
     9961 cataaaaggt ctagcggcag tagcggaaag tcggtgattt ctttgatctt ccttgcgagg
    10021 gtcgattttc ccgacccact ttgtccaatc acgatgatct tcattcttct ccctcacttc
    10081 tctttggaat ttggttcgat cataccactt ataacgccgc aaaaaaacaa gggactgtga
    10141 caaaagtcgt gtcaaagtcc cttgtttcga ataaatggtg ttcaatcagc tgaccttcag
    10201 cattagctca aaaagcggca aaatatgagg agctattttt ccgtttgttt ctctaatact
    10261 caggtcaaat cgctgatttt acaacctctt ccttaaacgg tgttctcctg ccaacacctc
    10321 ggtcacaagt cacaatagtc aacaaacatg agaagttgtt ttttgaaatt gctccttgtg
    10381 actcggagtt aaacggcagg ctcactacct ctttcttaaa cggtgttcca aagcttgcat
    10441 ctgcggtaat aagcccagcc aaacgaaaaa tatgaggggc tatttttgtt tggctgttct
    10501 tattacttga tgcaggacag ctttttcaca acctctgatt cacggtgttc cagaagtagc
    10561 ttcttcgaaa aacaagccga aacttcacaa aaatttgaga agcaattttc gtaaattcct
    10621 tcttatttct tgaagctgac cacttctgtc acaacctctt gtaacaagta tttctatttt
    10681 aaggctgaac cactgtttct ggttcagtga tttctttgtt tttgtctcga cgagcaattg
    10741 ctggcaagat catacctaat aagatcaaga cgattggtgt gatgatattc gtaaataatt
    10801 ggaaccacca tgctttagga tcagcagtat aatctacttt tggcaccatt cctaggatac
    10861 atgcaaacgc agtgaaggca aagcaccaag caccaaagat aaaaccgatt tttggatttt
    10921 taacaaattt atattcagaa ttgaatttct tataagcttt attcaacatc atatatgcca
    10981 agaacaccca taggtaacgc attggcatga caactgagtt caagttcgtc aaccatttta
    11041 ctaattcatt catatctttg ataccaaaaa tcggcaacaa aataatgatt cctactagta
    11101 tacctgtcag aagataacca ttcaccaatg tacctttacg attacgttta cgtagccagt
    11161 ttggtacaaa ttctggatcc gcatctgcta acaagatttt tagtggtgca tcaattgaaa
    11221 aggctaatgc ggagatttgt ccaatcatgt ttgtcaatgc gtaaatgatc atcaatgagt
    11281 taccgatacc atagtaatta cctaaaattt gaaaagcact gtatgcccca tttgccatca
    11341 agtctttagg aatattatca ctagcaaaca gcatacccat tgcgactgag cctaatacag
    11401 cacaaatccc taccattgct gctaagaaga tcatcccttt aggaaattcc tttgcaggat
    11461 ttcttgtaga gttgacataa ggggatattt tttccgcccc tccaactgca aatactagca
    11521 tcgaaacggt tgtgaaataa ctgaaatcaa atgttggaat atatgttttc acttgattca
    11581 tatgtggtgt ggcaaattcc atcgttggat tgatgaacgg tgccgttaca gctaaaagaa
    11641 taaataagat cgacatgacg aacatcgctg ttccggccag tccgccaatc actttcaaag
    11701 tagaaagtcc ttttgttgat aaatataaaa aggcaagaaa tataataagt gaaataactg
    11761 ctaccatttg tactgacatc gtatttacga tatttccatt tccttggact gcccaaccac
    11821 cagcaatcag aattgcttga ggcttttgtg ccaaataagg aatatggacg acccagtaag
    11881 tccaagcggc atagtatgcc agtcgtttat tagatgtcgt attttcgacc catgaactga
    11941 cgcctccttt actatccttg aaggctgatc ctaattgtcc aacgatcaat gcgtatggaa
    12001 taaaataaat ggccaaaatc aatagccaag aggtgacaac tgatactcct tgctgagcat
    12061 agttattaac tacattcccc atgccccaaa ccatgttaaa tgcaattaac gcaacagtaa
    12121 accagcgtaa ttgtttgttg ttcattcgtt ctctccttta tgataaaaat ttgttatcca
    12181 gatgtaatca tattcaattt ttaacaaact tacaagacag tgttatttta tgtgtgtatt
    12241 cttttattta atacacatcg ttttttcata ttttttccat caaaaaaata agttattttc
    12301 ttttatttca ttgaccttat tgaatttcaa gctattttga attttctgca aaattaaaaa
    12361 cattagttat tttataatcc tatctatttt ctttacattt ttcattaaga aaaggatata
    12421 aaaaaaagag gacgttcatt cgtcctcttc attgatcaac gtttattttt tacatgcaag
    12481 cttcgcatgg cattcaaaac agctaatact gtcaccccaa catctgcaaa tactgcatct
    12541 cccattgatg caaaaccaaa agcacctaag gcaagtacca aaatttttat cccaatagca
    12601 aagacaatgt tttgtttgac gattttcaat gttttctttg ctaaatggat tgcttctgca
    12661 atcttcgatg gttgatcatt catgatgact acatctgctg cttcaattgc agcatcgctt
    12721 cccaatccac ccattgcgat tcctacatct gctcttgcta gcaccggtgc atcgttgatt
    12781 ccatctccga caaatgcggt ctttttattg tttctttgta agatttcctc taaccgctgc
    12841 actttatctt caggaagcaa ttcgctatat atcttgtcca cgcctacttg cttcccgata
    12901 tgatcagcaa tttttttcga gtcgcccgtc aacatgactg tgttcttcac accagcctgt
    12961 tttaattgaa ccaatgcgtc tacggcatct tctttcaagg tatctgcaat aacaagataa
    13021 ccactgtaac tttgatcgat cgctagataa agcaatgtac cgatttcttg acttaaagga
    13081 aaagagattt caaatctttc catcaatttg tgattacctg ctaatacttg tttgccatca
    13141 acttcagcta agatcccttc acctgcaaat tcctggatat tagttgttgg taaaagcgga
    13201 cctgcgtagc cttctaaaac agattgagca atcggatgtg tagaaaactg ttctgcactc
    13261 gcaatatatt gcatgaattt ctttggatcg atttttgtat cgatcgtctg gatcgaaaag
    13321 tcacctttcg ttaatgtgcc ggttttatcg aataccactg tctcggtttg cgcaagcaac
    13381 tccaagtaat ttgaaccttt tactaataca ccgattttac ttgcgcctcc gataccgcca
    13441 aagaaactaa gtggcacgga aatcactaat gcacatggac atgagatcac taaaaatgtc
    13501 aatgctcgat agatccattc agaaaacgcc gctccagaaa caacaagtgg aggtaaaaca
    13561 gctaataaaa cggccaatcc tacaacgact ggtgtgtaat agcgcgcgaa tttagtaatg
    13621 aaattttcag ctggtgcttt tttactgctg gcattttcta ctaattctaa taatttgctg
    13681 atagtagaat tttcataaga tttttctact tgaactaaaa atggtttatt tttattgata
    13741 aatccgctta atactgtttc accaacatat acagatttag ggactgattc tcctgtaagt
    13801 gctgacgtat cgactaatga ttctccttcg atgatcgttc catctaacgg tacacgttca
    13861 ccaggcttca caagtacaaa ctgaccgatc tgcacgtctt cagggttcac ggtcatcaat
    13921 ccttccgtcg tctgcacgtg agctgtttct ggacggatcg ccatcaattc gcgaatcgat
    13981 tttcttgaat gactgactgc caatccttgg aaatactcgc cgatttgata aaatagcatc
    14041 actgctacgg cttctggata ttcaccgatc aacatagcgc caatcgtagc aacagacata
    14101 agaaaattct catcaaagat ttttcctttg acgatattca tcactgcctt cttcactaca
    14161 tcatatccta tcagcagata aatcaataaa taccctatca agcgaattgg ttcattaaaa
    14221 tcaaataata gcaataaagc gatacctgca agggaaaata aaatccggaa cagcgtattt
    14281 ctctcttttt catttccatg ttcagatact ttttccttat ctatttctgt tactttgaca
    14341 tctggttcta attccttcac tacttttttt gtttcttgtt caatcgtagc caaatgatct
    14401 tcctctgcgt caatcgttag ttttccagaa gtgaagttta ccgttgcttc cttgactcca
    14461 ttgatcttct gaactccttt ttcgattttc attgcacagt tcgcacaatc taaaccttct
    14521 aatctgtaac ttttcatttt aatgacctcc tcgcctcaac atatgaatat ctcttcataa
    14581 ctaattataa atgaatggtt attcatatgt caaggtatcc gaaagaaaaa atgaaaaaca
    14641 tctgcatcgg aaaatttctt ccatacagat gttttggctt attctttctt tttctcttcg
    14701 atcattgccc ctgctcttat ccaacccgca cttgccttct tgatcttgac aggatagcaa
    14761 ctaagtttga agcctgttgc tggcaatttg tctaaattca ttaatttttc gataggacaa
    14821 tatctttttt caatccctgc aaaatgtcct tcccagatat tcgaagctcc actacgcttt
    14881 caaaaatata gaaagagttc tttaatgaaa attatttcaa ctttccataa acagtaatcc
    14941 catataaata aaagacgtaa aagtcacatg ggagaactta tacaggaaaa cacatagagt
    15001 caggtgaaat acatgtatcc cacctgactc tatgcgtttt ctaaccttta ttattctctt
    15061 tttatacaat ttctgcaccg aatggcatca acgccaatcg agcaagtttg aaagattgct
    15121 tggcaaaagg tatccctatg atcgtgatac atagaaggat gccgctgacg acatgtgcga
    15181 gagcaattgg taggcctgaa acgatcagcc agatgatatt aacgatcaat gacatgccag
    15241 actggtgata aattacttct ttgccaaaag gcgcaaaaga aagggaggcg atcttaaaac
    15301 attgcatacc gattgggata cctatgatcg tgatacacca aagtatcccc gctgctaacc
    15361 agctaagacc accgattagt ccgccgaata taaaccagat gacatttcct aaacaactca
    15421 ttgtatttct ccttctatta ctgtttttga ttaaaactgg gcaaaaattg attttattct
    15481 tcttttttta gcttccagca tcaagtattc tgcaaatttt ttttggattt cttccgtatt
    15541 ttcttttgtt actaatgtgt gttggattag ttttcctgat ggtcgctgat ccacccgttc
    15601 ttctgactgt tgcacgtccg cttgcgcaaa tccgataatc cgtttttctt ttgttacagc
    15661 aacgagcaat agtttattct tatcatttag gtcagctgcc gtttttgctt ttacgaatga
    15721 ttctggatga tcagtaatca gctgattgat ttgaggtaaa tcttctttta ctgcccaccg
    15781 tatttccatc atttctcact tttctttcgc tcatttttat ctccactaaa aaaatcgact
    15841 gcggcaattg catctatcca gctttgtgca atgatctcat gtccgccaat cgttgggtgg
    15901 accccatctt ctcttgtata tttcgaaaaa ccataagcaa tccctaaagc attgaatatg
    15961 ccatctaaag gaaccagcaa tgcgccatat tccttagcaa gtttacgaac aatttggatt
    16021 ttagggtcta agtctttacg ccagccatga cggtcgtcgg ttataggaag tacaaacggc
    16081 tccatcaaga tgatgttgca gatttccgct gtttgaagtt catttagtga ttttcgataa
    16141 acggattcaa attgctgcgc agatttctca gttccgaata tagcctgatc gctagtatta
    16201 tgccaggtat cattgattcc gatcaaaatc gatacatagt caggttgtaa agctaaacaa
    16261 tcttcttgcc agcgtacttg cagatctgca attttgtctc ctcctatccc acggttcatg
    16321 cattcaaatt ccatcgttgg atattgggag attagttttc cggcaatcaa aagtggataa
    16381 cctttaccta gatcagttgc ttctgctcgg tctcttcctg cgtctgtaat gctgtcaccg
    16441 ataaataata tacgtttttt catttgtcaa aaactccttt ttgaataaaa cgatgatttt
    16501 cttccttata ttatcccata agccctatca agacgaaaga gaaaatatat gtataaaaaa
    16561 tgacaccagc ctagtccctt caatcaattg aatgaacgct catcaagtgt ctagtttttt
    16621 atactttttt tcttaacgtg ccggattccc cttgattcct tcgttgtttg gtccagagaa
    16681 atcgatcaca cctaagcttc cgtcaatcag accactgatg acgccatcag aacatatatt
    16741 tataggctca tctaagactt ccagtaaatg ttcttttact aatatctctt ctagattgct
    16801 tgggatagat tggtcataac tttatctggt ccgatgattt caaggatgaa ttaaacttac
    16861 atatggatca ttgcatgctg aaaatacgga cctactccat cttcgaatgt tactattagt
    16921 ttgttttcag gtactagatg ctgctttatt tcaacagcag aagcatcatc aaatcagatt
    16981 tccatttgaa taaccttctt tgtatatgtt tgattgattg cgatttcgtt atagaacctt
    17041 gtgaatcaaa tagcaatttc ttttgctttc ttctcttgtc tgctggaaac ttcctttatc
    17101 ccgtatttta aaaaaagaac tgcgagagtt cgcagttctt ttttttaaat aaacgttgct
    17161 ctccggccaa ctcctcggtc acaagtcaca atagtcatca aacatgaaaa actgtttttt
    17221 gaaattactc cttgtgactc ggagctgaac ggcaggcgca caacctcttg atataaacgg
    17281 tgttcaatca gctgaccctc gatattagct aaaaaaacgg taaaatatga gaagctattt
    17341 ttccgttttg tttctctaat actcaggtca aggcactgat ttcacaacct cttccttaaa
    17401 cggtgttcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat gtggaaagcc
    17461 atttccacaa aatttttctt aatactcgaa actaaacagc tttttcatca cctcttatgg
    17521 ataaattcgg ttcaacaaac gtgggaatgg aatagcttca cgtacatggt cgataccagc
    17581 aatccaagtg actgttcgtt caaggcctag accaaaacca gagtgcggaa cagaaccgta
    17641 tttacgtaga tcaagatacc atgaatattc tttttcatcc aaaccgtgat tacggatttg
    17701 ttctagcaag aaatcatagt ctgttgcccg ttcgcttccg ccaataattt caccgtaacc
    17761 ttctggtgcg atcatatctg cacaaattac cacgtcttct cttgttggat gtggtttcat
    17821 gtagaatggc ttgatggctt ttggatagtt taggataaag acaggctgtt cgaatgaatt
    17881 cgcaatgaac gtttcatgag gtgaaccaaa gtcttcgccc cattcgatgt catcaaatcc
    17941 atttttcttc agtaattcga ttgcatcatc gtaagtaata cgagggaatg gcaattttgt
    18001 ataagttttt aagacttctt tgtctcgtcc tagtacatct aatgcatatt cgcaatgatc
    18061 taaaacattt tgtactaaga atgctacata ttgttcctgt acttctaaac tttcttcttg
    18121 atgagtgaaa gccatttctg gttcgatcat ccagaattcg atcaaatgac gtcgtgtttt
    18181 tgatttttca gcacggaaag tcggaccgaa tgaaaagact tttccgaaag ccatagcagc
    18241 agcttccatg tataattggc cactttgaga taaataagct ttttgatcaa agtaatttgt
    18301 ttcgaataga tccgtagtgc cttctggtgc agaaccagtc aagatcggcg gatcgatttt
    18361 gacaaaatga tgttgattaa agaattcata cgttgcacga atgatttcat ttctgatctg
    18421 cataatcgca tgttgtcttg aagaacgcag ccataaatga cgatgatcca tcaagaaatc
    18481 tgttccgtgt tcttttggtg tgatcggata atcgtggctc tcgcttacca cttccacatt
    18541 tgtaattccg atttcataac caaattttga acgactgtct tcacggattt ctccagttac
    18601 taacacggat gtttcttggt gtagattttt agctgtttgg aaaacttctt ccgacacttc
    18661 gctttttaca actacacctt ggaaaaaggc tgttccgtct cttaattgta aaaatgcgat
    18721 ttttccgctt gatcgcttat ttgcgaccca agcgccgatt gttactgttt ctcccacatg
    18781 gtttttcgaa tcgatgattt gaatttgttc cacgtatgac tctcctttta tattccttga
    18841 atttcttcag gattaatttt gacttttact ctcaataaat tgcttgattc tgcgtaccgc
    18901 ttcttctaac gtagcgcgat cagtggcata actcatgcgg acattttctg gtgccccaaa
    18961 gccttcgcca gtcacaagcg ctacccctgt ttcttctaaa agatcttcta cccattgtgt
    19021 gacattttca tacttacaca tgcgtagcgt ttcttttaca ttaggaaaaa gatagaaagc
    19081 tccttgcggc ttttcaagag aaacacccgg cagttctgcc acaagcggat ataacgtatt
    19141 caggcgttct tcaaaggcgt tccgcatttc ttctactgta tcttgctcac ctttcaatgc
    19201 ttcaacagct gcatattgac tgactgctgt cggattgcta gttgattggg aagcaacagc
    19261 gatcattcca ttgatgatca caggatttcc caccgcataa ccaatgcgcc agcctgtcat
    19321 cgcatatgtt ttagacacgc cgttgatgat gatggtctga tttttgattg cttctgaaat
    19381 cgttgcaatt ggagtaaact cattgccatt ataaactaaa cggccataaa tatcatcagc
    19441 aactatcaaa atatcatttt ttactgccca ttcaccgatt gcctgcagtt cattcttgct
    19501 ataaatcatt ccagtcggat tcgatggaga attgatgatt acggcttttg ttttttctga
    19561 acgtgcttgt tctagctgtt caacagtaac tttccagcta ttggattctt cccctttcac
    19621 aaagacagga agtccttctg ccagttttac ttgctcaccg taactgaccc agtaaggcac
    19681 aggaatgatg acttcatctt gcgggtcaag gatcgtttgg aaaagggtat acaaagcaaa
    19741 tttcgctccg tctgttacga ctgtttgtga aggttcatag acaagtccat agtttttctt
    19801 cagataatca acgattgcct gtcgaagctc tgggattcct gctgttggtg tataatagct
    19861 agctttccca ttgcggattg cttcgattgc tgcttcttgg atgttttctg gtgtcgcaaa
    19921 atccggttct cccacagtca aactcagcac atctctgcct tttgctttta atgccttggc
    19981 ctttgccgca gctgccaaag tgacagacgg ttctaatcgt tttgttcttt ttgataactc
    20041 catcccattc attcctttac cttttaaatt cccttgattg tcttgacttc atctccagat
    20101 tcatagccaa tcaggtaata attcagtttc ccatttgttt cttttcctac tacttcccac
    20161 acaggctgat catcatacat ccctaaagca gctttttcga tttggatttc aggatgggcc
    20221 gcagcaattg tctgttttgc ttcattttca gtcagtccct ttgtttgatc caatactttt
    20281 actttttcac cagatttagg aatgataacg gcgatttctt ttcctttatc atttgtacct
    20341 gtcagtgaaa agtatgtctt ctttcgagta aaccaataaa aatgatcggc ttcctcaaga
    20401 tttgcatatt tctttgcgat ttctactgct tctttcttcg cctgcaccgc cggacgactg
    20461 gaacgcaagt aaaaaatcga gctgatcacg atgaccgcta ataaaaatac gatcaagcca
    20521 agtaacacgc gattaatcgt tttttccttt gtctgttttt tcttcaaggg tcgtcgctcc
    20581 tttatcttca ttccttttca ttatagcaga ttttaattat gaaaaaagac agctcctcta
    20641 acttccttct tcatttttct ttaagaattc atggatatcc tgatagattt cctccatagc
    20701 tagctctttg atcggtactt ttttcggtaa tgcatggatg atccgtgcac catattttgc
    20761 agtaactagt cttcgatcca aaataagcaa tacgccacga tctgtctccg tccttatcaa
    20821 gcgtcctaat gcttggcgta acttcaaagt tgctttaggt acggattcct ggtaaaaagg
    20881 attgatccct tctgattcta aataagcgtt cctagctttg accatcgggc gaagcggatt
    20941 atcgaagggt aaacgtgtga ctacaacgat ctgcaaagca tctccaggca aatctacacc
    21001 ttcccaaaaa ctgtcagcgc caaacaatac gcttttttct gacaaaatga agcgtttcaa
    21061 aagcttctcc cggcttccgc caaacccttg agctagtatt tctcgtcctt tttctagcat
    21121 agacagatgc attttctgat agaccttctg caaaacatca tgagaagtga ataacacaag
    21181 tattggccga tcttcttgtt cgatcagacc tgtaagtgct gtctctaaat aatcgatata
    21241 tgcttgattg ctgacttgat ggacggcagg tgcctctttt ggcacataga tccttgcctg
    21301 tttctcataa tcataaggag aggcaatcac tttaagaggt gctgcttcaa tgccaagcct
    21361 cttagagaga tagtgtcgat tagggccgat cttcagcgtt cctccaatat aaagaatacg
    21421 atcatatcga ggataccaaa cagtcgacgg caacatagcc gcttgaaaat catggagatg
    21481 aaaaatcatc tgctgcgctg tgttcccagg aacgaaccaa tggatcaaat gatctcgcca
    21541 gtcttccagc cattgatcca tcagcaagta ctgcttgtct aattgatcaa ataggctata
    21601 taaacttgct aaatcgatcc gcatctggtt tgtccagctt tcttggataa tctcaaaagt
    21661 cagacgcatt tgtgactgta gctgctggat ctcctgataa aaaagctgca ggtgatcaat
    21721 ggctgtccca gattccagtg gcagctgatc caacatctct tttgtcatca tgacttcttc
    21781 tggatactgt ccgttgggtt gcttgccgct cactaaactc attaatcctt ctgccataca
    21841 aatctgtgct tcgcatagcg cagaaagttc ttctcgataa atcgataacc agcgtaaggc
    21901 ttcatgttct ttcggtaaat agctttgtat tcggtcaaac agctgatttg gttcttctaa
    21961 ttgctggatt tgtttatgga aatagttaat acttaatcgt ttgtcattta ctttttcagc
    22021 gatttccggc aaatgatgta cttcatcgat caacaaaaat ggactagctg gaagcaatgg
    22081 ttcttttcgc tgtgtttctt gtactaaaaa cgcatgattg acaatcaaaa aattactttg
    22141 cctgattttc ttttgcaaga aacgaacaaa gtcttgttcg taaaatgact gatttcttgc
    22201 taaaaatcca atacctctgt gcgcgatatc tgcaaagaat gaatggttca aatttgttaa
    22261 atgtaattca tccaaatcac cggtcttcgt ttgcgtcagc caaactaaga ccgccatttg
    22321 atacaaagca tattgttttt gttctggcgg ttccacaagt gttcctttaa atcgttgcag
    22381 gtcgatatag tggcgatagc ttttgataat tacaggttgt atgggctgat ctagcaattg
    22441 attcagcaat ggaatgtctt tttctatgat ctgctgctgc aaaactagcg aaacggtact
    22501 gatgacagcg ggtttttcag gtgtagccaa ataactcaat ggtagcagat aaccgattgt
    22561 tttacccatt cctgtagctg cttcgatcat caaatctttt gattcatctt ttgtgaaatg
    22621 gtcgtagacc gcattcatca gtctgttctg ttcttttcga tacatcagtg ttttaccaaa
    22681 cattttttct tttgcttgtt tttttcttgg atatgtccgc tcgccataat ggacggatgt
    22741 gaatagttcc acttcttttt tctgtaaagc tagcccatcg acgatttcta acgagggatc
    22801 aagtggctct ggtttttctt tcatttcttc aatcacatga taaatgaaac gactcgtatc
    22861 catacctgtg tgtctactta atcgcgcaat ctgctctaac gtgatgattg gcaattttct
    22921 catccgttct tcgatcagca aaagaagttg acctgtcact tcggcatcac tatctgcctg
    22981 atgaggattc tcatgaataa atccaaggct ttctgacaaa tcagctaagc gaaagcttgg
    23041 ttccgtcggc aagaaaattt gtgcaagttc tactgtatcg atgcctggta tcttcaaact
    23101 aggaagtccg catcgcatga gttcatggtt caaaaaatta taatcaaaat aaatattatg
    23161 cgcgacaaat atggtgtctg ccaaaagatt gtaaatcgtt tgggcgacat cttcgaaata
    23221 aggcgctttt tggacgcgtt gattcgtgat ccctgttaaa tgctggattt gttttgaaat
    23281 cttccgccct ggatgaatat cgatcgaaaa acgtgaagtg atccgacctg attcaatcat
    23341 gacgcagcca aattggatga tccgatcttc ttttggattc gtcccagtcg tttcgatatc
    23401 aacgactgca taagttcttg gtttcaaaat atcttcctct tctcctgcct ctaatgttct
    23461 tttgaaatta tactacaaag tgcagaaaaa tactctcatc gctttttaaa gaaaatagac
    23521 aaaaacgccc agacacgcaa atgtctgggc gtttattttt tagtataggt taattaagct
    23581 ttagtaacgt ttgttgcttg aggtccgcgt tggccttctt caacatcgaa agttactgct
    23641 tggccttctt ctaaagtttt gaatccgtcg ccttggatag ctgagaaatg aacgaataca
    23701 tcgtttccgt tttctgcagt gatgaatcca aatccttttt ctgagttaaa ccattttact
    23761 gtacctgttt ccataataaa aatcctcctt gtgtatcaac acatattttg caattacttg
    23821 aatggtgtat atctggaaga tgtttatttg aaacaagcta ccacagatta ccgaacaaaa
    23881 actactcctc tagtatatca taccgtttac aaaaaagcta gagtttttcg acttttttta
    23941 ttaaaaaaag ttttctcagc gattacataa agcgaaaaac cgaacttttt ttaaaataaa
    24001 cgacatattg ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt
    24061 attctttctg actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag
    24121 taggtctgat gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc
    24181 aaaccaatac gatcaacgag attagttttt cttttttgct catatctata cctcataaat
    24241 aacaggatga tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga
    24301 atgtaagata tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa
    24361 tcttggctgc aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctgttgtctg
    24421 tacataaaca tttttgtaca gacgtgtgcc tgtaatagga gaccgctgtg tttcttcagc
    24481 aaaaactggc tgacttgtta aacttaggga tagaaacagc aaacaccata acagtctttt
    24541 tttcataaag acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc
    24601 ccgttattca cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt
    24661 cgtgaccacg tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata
    24721 agccgaaatt tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa
    24781 gctgaccact tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt
    24841 aataagccca ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg
    24901 atgcagaaca gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta
    24961 ttagctcaaa aaacggaaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca
    25021 ggtcaaaaca ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt
    25081 catctggcgc atctttttga tccaaaataa acactttccc gccattaatg atcacatcat
    25141 ctgctaatgc attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac
    25201 catcagctaa gtttgacgta gcgataaata aatgtgacac ttttccttct tttgcagctg
    25261 gaacgatatc aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg
    25321 attcttgagc tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg
    25381 atagctgcgc aggagaagca gaaatcgcaa cgtctgcaca atagtaagga tttttggcga
    25441 tttttttgaa catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg
    25501 gattttcgaa agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga
    25561 tctctacttc ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc
    25621 ctcctccatt agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa
    25681 cgacatcaac cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt
    25741 catacaattt catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc
    25801 catttttgat aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg
    25861 ggatactcaa acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag
    25921 ttgcactacg ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg
    25981 accagtcatg ttcggaatat tttttctcaa aacgtttctt tgcttctttc gaaaaatttt
    26041 tgaatttgat ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg
    26101 tgacgaatgg tcctttagcg ccttctgaaa tcaattgtga caataattct ttatcctttt
    26161 ttgccataaa cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca
    26221 caacactatt tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt
    26281 taaatttagc tattttttta agttatcttc ataatttcct caattattta gccccagtct
    26341 tcaactattc ttcatgaaat gctgataaac taagaaaaag aataaaagag gtgtatctta
    26401 tgcatttgat tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt
    26461 cccgcttatt agaggcggaa acacagcctg ttcctataaa agtacgaaaa aatgaccgct
    26521 aaccttttca taacgcgcgt tcttatccta aagttttttt cagaaacatt gaaactctct
    26581 tccttctctg ataaaatttt aatttatagc gaaacaaatt aaatacaagg agatcggata
    26641 tgtccataaa aaaaacacgt ctagtcctat tattggctag tctcattctt tgccttgctt
    26701 cttttctacc agctgttgct gtgcaagctg aagatacgtt caaagtaaat gcaaaagcag
    26761 cttttgctgt ggatgcgcaa agcggtaaga ttctttatga tcaagatgga gaaaaaacca
    26821 tggggatcgc ttcaatcaca aaaatcatcg gtctttacat cgtccttgat caagtaaaag
    26881 aaggaaaact atcatgggat gataaagttt cgatttcaga ttatgcagaa aatttgagta
    26941 tcaccccaga tttatcgaat gttcctttgc ataaagaaaa cacctacact gtcaaagaat
    27001 tattcgactc tgcaatcatc cagtcagcca atgcttcaat ggtggccttg gcagaaaaag
    27061 tctctggcag cgaagctaaa ttcactgaac gaatgaaaga acaattgaaa gattggggaa
    27121 tcaaagatgc aacgatcgtt aatgcatcag gattgaacaa ctcttatctt ggcgaaaacc
    27181 gaccagaagg cactggagaa aacgacgaaa atcaaatgtc tgctcaagat gtggccatcg
    27241 ttgcccgcca tttgatcttg gatttcccag aaatactgga tgtatcaagt actacgaccc
    27301 aaatgtttgg cgaaaatacg caatcaccag tggaaatggt caactggaac tggatgctcc
    27361 caggattcgt taattacaag gaaggcgtgg acggattaaa aactggtacg actgagcttg
    27421 caggtgcttg tttcgttggt acgatcacta aaaacgatca acggatcatc actgtcgtat
    27481 tgaatgcgga cggccatgcg gaaaacccga gtgcccgctt caatgaaaca gaccgtttga
    27541 tggactactg ctatgacaat tggtcagaaa aagagctcgg aaaagctaac gcaagtattc
    27601 ctagcctaaa aaccattgat gtaaaagacg gaaaagaatc aacagttcct gttgtgctta
    27661 aaagccctgt aaaagtatgg gtacgaaacg atatggatac tggcaaatta acgatcacgc
    27721 caacacttga caagaagcaa gtcactgaca acgagatcca agcacctatg gataaaggta
    27781 caaaaatcgg taccgctacg atcaccttgg cagacgataa attaggttat ttagaagata
    27841 acaatgcgcc atcgacagag atcattacgg ataaaacagt ggaaaaagcg aatatgttcg
    27901 tgattggctg gcgacgcgtc gcagaatttt tcggcaactt gttttaattg aacaataaga
    27961 aaagcatctt tccatcggaa agatgttttt ttgctttttc tagtttaata acagcttttt
    28021 tcatctattt tcgttatact gtataagaaa aaggagttga atttatgaat ctaaccagag
    28081 atctgatctt tttagttctc tatcttattg gggtattctc tatcgtttat tggcgaaaac
    28141 agaagaagga tccttccttg ttcttttacg gcagtacgtg gacatttacg gtaatcttaa
    28201 cttattttgt cgtgttggaa attttttatt cgacctttta ttttttgatt ccattgttgt
    28261 tctttagtat ctttgctttt tcttatttca cagaaaaacg aaggctgctg aatggtcttt
    28321 tattcaatgt tttcctcatc agtttcggca tctacttgtt tgtcttgctg tatgaaacgc
    28381 aaaatatttt ccttggcgga ctgatcgcct tgatcacgat tccattgcta cttgtccttt
    28441 tatttggtat ttatggattg attgtttttc ttttttggaa cggggtcaca gttttacgta
    28501 gagaatcgca ttcattagct aatcttttaa cgttgatact agccattttt cttacactgt
    28561 ttcttgtttt tgactttttc ttgttaaaat atttgccgca atggataaat gctttgtttt
    28621 tctgcgttcc cctgatcttg atctatctat ttatcgtatt ctacaacttt ttgactgtct
    28681 cattcttgta tcagttcaac cggcctcgat acaatcaaga cttcatcgtt gtgttaggcg
    28741 ctgggttgat caatggagaa acagtttctc ctcttctagc caagcgaatc aataaggcga
    28801 ttgcttttta tcgggctcaa agccgtgcta cattaaatcc gcccatctta ttgatgtctg
    28861 gcgggcaagg agcagacgaa aaagtaccag aggccattgc gatgaaacaa tatgccatgg
    28921 aacaaggaat ccctgaaaga gacattcttg tcgaaaccaa ttcgacaacc actttggaga
    28981 acatgctcta ttcaaaggaa atcatggatc agcaaatgaa agggccatac cgcgtgatct
    29041 ttagttccaa caattaccac atttttagag ccggtcttta tgcgtgtcaa gccaaattga
    29101 aagcaaatgg tattggtgca aaaacagctt tctactactt gcctaatgcg tttttaagag
    29161 aatacatcgc catcttgatg atgaacaaaa aaagacatat gatcatttgc ggattgcttt
    29221 tcgcaggctc cgcattctta tcgctgatta ctttactttt ctaaaaaaac aaggtgtgac
    29281 aaaacatatg tcacaccttg tttttttctt tcattctctt aactattggc aaaaagagta
    29341 tccctttttt gaccgctact tgctgagcag aataaatacc gctgttgcct gaacacataa
    29401 agctgatcaa gcagatcata aagaagtata ctgcagcttg tgaaccgaac aattcgatcc
    29461 ccatcacaaa gcaggcaatc ggtgtatttg tcgcaccaga aaatactccg ataaagccca
    29521 atcctgcaag aaaaggaaca gaaacatgga gcaaaggcgc tagtgctgca ccaagcgttg
    29581 caccaatttc aaacaatggt gtcacttctc ccccttgata tcccgcgcct aaagacaaaa
    29641 ctgtgaagaa taacttccca ataaaatcat agacatgtgc ctgtccatta aatgattctt
    29701 gaagtaacgg aagactcaat ccgagataac gttgtgtttg caaaatcaat gctgctgcca
    29761 caactacagc tccacctatc gtatttcgcc aaacaggatg aggaatccat tgtgcatatc
    29821 gagcttttaa aaagacgatc gaacggctga atacccagcc aatcaaacca aaagcaatcc
    29881 cagcaacaaa aactttgaag aacagcaaca tactccattc aggaatcaca cccatttggt
    29941 aatgtagatg agaaacacca tagctttctg tcatgaaatt tgcaaacagt gcagcaaaga
    30001 agctagggaa gatcgcttct gttcggactt ttccgattgc caacacttct aatccaaata
    30061 atgttccagc taaaggtgtg ccaaatacag aactaaaacc cgcactgatt ccactgataa
    30121 tcaagatttc ccgttctgat ttatccaatc ggaacaaacg tgctgtatta tctgctaacg
    30181 ctccccccat ttggactgct gttccttcac ggccaacaga tcccccaaat aaatgggtag
    30241 ttatggttcc aaacagtgta agtggaataa gtcgcaacgg gattttttct tctccaccat
    30301 ttccttgatc gatcactaag ttgtttccac gactagagag accgccgtga tacacataga
    30361 gataagcaaa aagcgcacca ctaactggta gtagataaag gagccaagga tgtgatagtc
    30421 gggtatctgt cacaactgtc aagctattta agaaaaaagc agataagctc cccatgaata
    30481 taccgagaat cgctgtgatc accagccact tcagcatata cactaaaatg cgcattggac
    30541 tcggtaattg tttttccata aaaaattcct cctcaataaa aaaacgccta ctaaagaaag
    30601 gggctgtccc cagactttag taggcgtcat tagctatctt tcgttagcat ttaaaggcga
    30661 acaccatcac ctattcactt ccattaaaag atagcatatt tttctgtaaa aaacaataaa
    30721 attatcgttc tttctttaca cgttcagcaa attcatagaa aggagccaat ttcgttaact
    30781 gttgattaca ttggtcacta tcatgacaaa tataattccc ctttttcgta tacgtgccat
    30841 cagaacctgc tttcgttgta gaaagaaaaa gcgaaacatt cgatgttttc tgacagattg
    30901 cacagattcc cttatttatc gttggtgata aagtaccact aatccctttt aatttttcat
    30961 cttcgtaata gaccatgaat tttttctgcg tacctggatc attccagccg atgtacgtat
    31021 gttcttgcaa gtcgatttca ttccaagctg gttgtttgag ttttttcact tttcggaata
    31081 atttctcgat ttgtggtttg ctaggctgca taaacggctg aacgatttgc ttcagctcct
    31141 ccaatgctct ttctgcttgc gcttttgtca gttttacgtc taaaactgtc gttaaaaatg
    31201 tatcgatttc tagcagttcg tgatctaata tgtctcgaat cttttcagct gctagtgcct
    31261 gaactgtttg taccgttttt ggatcattga cagatgagta cgcatttaat aactgttcta
    31321 cttctctttt tataaaaaaa tattggtatg gttttattgt ttgttccatg attttaaagt
    31381 cctttcttct aaccaaagtg catactttgt agaaaaaagt gatcttctgc aaagtatgtg
    31441 ttacctgaag atcccggact cttttaaata agatacgttt tgcatcttat catcaccccc
    31501 tttaatgctt attcttctat gtacatgatt cattcgataa ataaaagtaa accgtatctt
    31561 gaacagatag tcaactgcaa acagaaaaaa ggactgtgac aaaagtcgtg tcaaagtccc
    31621 ttgtttcgaa tgaatggtgt tcaaaagctt gcatctgcgg taataagccg aaacttcaca
    31681 aaaatttgag aagtaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt
    31741 cacaacctca atatggtcta ttattggttt tacctgttaa atcgaggatt ctttcccaaa
    31801 gtaacgttgc catctcttac gcatacttgc agacatgggc tgagccgctt ctttgactgc
    31861 gcagtatttg tccacgaatg tcacgatata tccttctttg tacttaggcg gtgcgatcgt
    31921 tgcaccccac atgtgcttca aaatgatatc tcgctcaaga tccgacaaat cggtgatttt
    31981 ttctgcattc tttacagcaa ttcttggatg gatatacgca tgtgttccct cgtcaaattt
    32041 cgtcgtacgc caatcatagt agaacagatc atgcaaaagt ccagcacgcg ctgttgcttt
    32101 agcattgcca ccccattttt ttgcgagcag ataactatag taagaaacac tgatggaatg
    32161 ctccaaacga gtggaatgaa catgctgtgt gtaattcgct aattttttca ctgcttctgt
    32221 ttctaaaaga tcttgtacat aagacatata ttcttcgtct tcacgccatt tttctgtcat
    32281 aattatcgat ctgccacctt tttgaatttc attttcttct gaaattgatc taagtatacc
    32341 accagtgttc ggattattct agtcgctcta gataaaatag ctaaagctta atagttcctt
    32401 aatcataaag aaaaatgata aagaacgatc cctgctgcaa ctccgacatt caatgattcc
    32461 gcatttcctt tcattgggat atatacgttc tgatcagtca aagacaagat ttcctgactt
    32521 actccctgac cttcgtttcc catgattata gcgaaatcct ctgttttttc tactttgtct
    32581 aatgcatgtg cttcttcatt taattctgta ccgtacaccg taaacccttt gcctttcatt
    32641 acagggatta catctgccaa atctttttgt aacacaggca aatgataatt gcttccctgc
    32701 atgcttcgca gcacctttgt actataaata tctgccgttc ctttacctaa taaaacacca
    32761 gccataccaa acgcatctgc cgtacggatc atcgttccca catttccagg atcctgtaca
    32821 ttatctagta aaagccagcc tcctgaaaaa tctggcgtat cttcagatgt taacgtcatc
    32881 actgctaaga ttccttgcgg tgtaggtaga tctgacatag cggtcatgac ctcatctgaa
    32941 actagaaaag tagaaatctc tttgtttaca gccagccagt ctccccattc tttcatacct
    33001 ctctgtgtca caaaaagttc ttttatttct gcatcagacg acgcagcttc ttcgatcaga
    33061 tggaaccctt ccaaaagata ttctgccttt tcttctctgt atttcttttt atgtagtttt
    33121 tttaattctt tgattcttgg attttttgct gattggatct ctttcacatt cctcacctca
    33181 actgattata tcatggaata ggtgcttgag cttgtttcat cctaaaaaaa tcggtatcat
    33241 tgatttataa aacaggaaag aaaggtgacg aacatgcgca aagttaaaat gaatgtccaa
    33301 ggacgtgtcc agggcgtggg atttcgctat atgaccaaga tggttgctga tcagttaggc
    33361 gtgaccggaa cggtaaaaaa cgaagacgac ggctcagtca caatcgaagc aatcggaaat
    33421 gacgacatca tccaaaaatt tatcgaagaa gtcaagaagt ctccgagccc aagtggacga
    33481 gtgcagtatg tggatgtcca agaagatccg atgattgagg aacgaaagaa atttgatgtg
    33541 gtgggataag gttgtttttg aagcgctttg cctcgagtat taaggagaaa cattttcaaa
    33601 atagcttctc atattttgaa atgtaatgac ttaataccga gaggcagctg ataaaacacc
    33661 gtcaataagg ttgcgaaaaa gctgatctgc atcaagcatt aaggactggt tgcgaaaaag
    33721 tcgcttagtt ttgagtatta aggaaaattt tgtgaaaatg gctttccaca tttttgcata
    33781 aatttgtctt aataccaaga aactgacttt tgaacaccgt cgataaggtt gtttttgagg
    33841 cgctttgcct caagtattaa agacaggtag taaaaaagct gatctatcac ttgcagtgag
    33901 ttcatttgac tcctctattt cgggcagaat aaaaaagttt ttagtttttt aaataagaaa
    33961 gaaacacttg gaatcactct tttctttcca agtatttctt tcttgatttt ttgtcttttt
    34021 tcaaaatttt tcgctacgaa aaagaaagaa tatttatatg aaatttctat gaagttgatt
    34081 gaattccatg cgctttttta gtatagttaa gtttgtgtaa aaattataga agaggaagta
    34141 ttctatgaat aaatggaaaa actggttatt gggatccgga cttgtcagta tcctgctctt
    34201 tttatctggc tgtgtgcgga tggattcaaa cggaaaccct gacacttcag gaatcgttta
    34261 tcgagtactc gtccatccta tgggccaagc gattacatat ctagttgaga atttcaattg
    34321 gtcttacggc tgggcagtta ttgcaatgac tgtgatcgtt cgtatcatca tcttgccact
    34381 aggtatcagt caatcgaaaa aaacaatgat ccaatctgaa aaaatgcaag cgttaaaacc
    34441 acaagttgaa gcagcacaac aaaagctaaa agctgcaaca acacgtgaag aacaaatggc
    34501 agcacaagca gaaatgcaac aagtatatcg cgaaaacgga ttgagtatga ccggcggaat
    34561 cggctgtctg cctctattga tccaaatgcc gatcttctct gccttgtatt ttacggctcg
    34621 ctacacagaa ggtatccgtg aatcatcttt ttacggcatc gatctaggct caccaagtat
    34681 ggtgcttgtt gccattgcag gggtcgctta tctgcttcaa ggatatattt caacaatcgg
    34741 tattcctgaa gaacagaaaa agacgatgcg tacgatgctg atcgtctctc ctgctatgat
    34801 cgtcttcatg tcgatcagtg cgccagccgg tgttacactt tactgggtag tcggtggtgt
    34861 gttcagctgt ctgcaaacat tcatcacaaa cgttatgatg aaaccacgtt tgaaagcaca
    34921 agtggcagaa gagttgaaac aaaacccgcc aaaacaagtc gtcactccaa gaaaagacgt
    34981 gacagaatca gcaaagccag ctgacaacaa acaagcaaca aaacaaatca aacaaccaaa
    35041 aaacagtaca aacaacaaag gccgcaacgc aggaaagcag cagaagcgga agtagatagt
    35101 aaataagagt gtaataaaaa ttcattaaca gttttagcaa ttaaaaatat cgaccaagat
    35161 ttttgaaaaa gaaatctggt cgatattttt atttaagtga atgtttaatt tgaatcatcg
    35221 attgatcgct atctaattgc agcagcatct ttttatgaag aaacaacttt ttcttatcta
    35281 tcaacgtaga caggtgttgc tgtttctttt aataatgtac ttaattcctt aatattttgt
    35341 tttccttctt ctaacatcca actactcgct tttttttcgc cttcggtaac aaaaggtgcc
    35401 actcccttcg cccatgttgc tcgcccacat aagataccat tgaatgtcga acctgattgt
    35461 tttgcaaaac gtaatgtatc ttgaaatagc gtagcattga ctcccgcact taaaaaaata
    35521 aacggttgtt cagtcacttt cgattgttca tagaaatacg cggatgcctc tgcttgacta
    35581 taacaagtct cttgcccata tccttccaca aatctcatat tcactggcac ttctactttc
    35641 aatacatcca cttgataacg ttcttttgaa aattctcgca tggcttcaat aactttatgg
    35701 ggttttactt ttgcataggc aagagaagac ggatcgccaa tagttgtatc ataagaaagg
    35761 atctctaaaa agaaaggtat gccttctgct tcgcattctg aaccaatccg ttctataact
    35821 atttgttttt gcacattaat ctctgctggt tcatccacgt catagtacaa taaaaattta
    35881 caagcatccg caccagcttc ttttaacctt ttcaccgacc aaatattcaa taaatcaggt
    35941 aatcttcccg gcattttcgt atcatagccg gttttttcat aagctagcag caagccacat
    36001 tctttctctc gaagcgctgc tgcaggcagg ccgtattctg gatcaagtaa aatagctgag
    36061 gcatagggag tcaggtattg agaaaccatc tgtttaaatt ggatgatgtc ttctgatgta
    36121 gcttctttat attggttgat cattttcttc aaagcaccac gctggtcgat tgctaacgcg
    36181 ctaataattc cagcttcatc cgtcaatcgc ttcaaacgtt cctgcttttc tatactcatc
    36241 ttcttcatat atcttcacct atttctttag ttaatattgt ggctattttt gtttttgatt
    36301 ttgcttttct caactgttct gtaacctctt ttcgcatcaa tgatcgtgca acgagagaca
    36361 ataatttcaa atgagtcgtt cctgcttctg aatctggaat aaataacgca aaaataaaat
    36421 agtaaatatc tactcgtgaa aacgggtggt ttttggcagc cctagaaggc ctcatcactt
    36481 gcttatcctc ttaagagggg ctttttaagg gaccaccaac ttgtaccgac ccccaaaagt
    36541 tagaccaaaa aatctaactt ttggaggtcg gtatttttta tggctaagta tacttttgaa
    36601 cttaaattaa agatcgttca tgattatttg gatggcaaag gcggaagtga ttatctggca
    36661 aagaa
//
LOCUS       DAHUVV010000019        35804 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.019, whole genome shotgun sequence.
ACCESSION   DAHUVV010000019 DAHUVV010000000
VERSION     DAHUVV010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.019"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            4..816
                     /locus_tag="NK767_000960"
     CDS             4..816
                     /locus_tag="NK767_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCH6693969.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="NK767_000961"
     CDS             813..1613
                     /locus_tag="NK767_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCH6693970.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="NK767_000962"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="NK767_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCH6693971.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="NK767_000963"
     CDS             3110..4819
                     /locus_tag="NK767_000963"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCH6693972.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="NK767_000964"
     CDS             5031..9383
                     /locus_tag="NK767_000964"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCH6693973.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="NK767_000965"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="NK767_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCH6693974.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="NK767_000966"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="NK767_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCH6693975.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="NK767_000967"
     CDS             11221..11514
                     /locus_tag="NK767_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCH6693976.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="NK767_000968"
     CDS             11511..11822
                     /locus_tag="NK767_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HCH6693977.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="NK767_000969"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="NK767_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCH6693978.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="NK767_000970"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="NK767_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCH6693979.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="NK767_000971"
     CDS             complement(14623..14844)
                     /locus_tag="NK767_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6693980.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17655
                     /locus_tag="NK767_000972"
     CDS             15292..17655
                     /locus_tag="NK767_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HCH6693981.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAKKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            18023..18946
                     /gene="truB"
                     /locus_tag="NK767_000973"
     CDS             18023..18946
                     /gene="truB"
                     /locus_tag="NK767_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCH6693982.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18950..19891
                     /gene="ribF"
                     /locus_tag="NK767_000974"
     CDS             18950..19891
                     /gene="ribF"
                     /locus_tag="NK767_000974"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCH6693983.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20243..21028
                     /locus_tag="NK767_000975"
     CDS             20243..21028
                     /locus_tag="NK767_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCH6693984.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21041..21892
                     /locus_tag="NK767_000976"
     CDS             21041..21892
                     /locus_tag="NK767_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCH6693985.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22016..22468)
                     /locus_tag="NK767_000977"
     CDS             complement(22016..22468)
                     /locus_tag="NK767_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6693986.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22933..23301)
                     /locus_tag="NK767_000978"
     CDS             complement(22933..23301)
                     /locus_tag="NK767_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HCH6693987.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23451..23933
                     /locus_tag="NK767_000979"
     CDS             23451..23933
                     /locus_tag="NK767_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HCH6693988.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            23970..24980
                     /locus_tag="NK767_000980"
     CDS             23970..24980
                     /locus_tag="NK767_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HCH6693989.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25095..25808)
                     /locus_tag="NK767_000981"
     CDS             complement(25095..25808)
                     /locus_tag="NK767_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693990.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(25998..26588)
                     /locus_tag="NK767_000982"
     CDS             complement(25998..26588)
                     /locus_tag="NK767_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HCH6693991.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27072..27299
                     /locus_tag="NK767_000983"
     CDS             27072..27299
                     /locus_tag="NK767_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCH6693992.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27315..28481
                     /locus_tag="NK767_000984"
     CDS             27315..28481
                     /locus_tag="NK767_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCH6693993.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28715..29758
                     /gene="hrcA"
                     /locus_tag="NK767_000985"
     CDS             28715..29758
                     /gene="hrcA"
                     /locus_tag="NK767_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HCH6693994.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29852..30415
                     /gene="grpE"
                     /locus_tag="NK767_000986"
     CDS             29852..30415
                     /gene="grpE"
                     /locus_tag="NK767_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCH6693995.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30466..32295
                     /gene="dnaK"
                     /locus_tag="NK767_000987"
     CDS             30466..32295
                     /gene="dnaK"
                     /locus_tag="NK767_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCH6693996.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32446..33612
                     /gene="dnaJ"
                     /locus_tag="NK767_000988"
     CDS             32446..33612
                     /gene="dnaJ"
                     /locus_tag="NK767_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCH6693997.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(33694..34359)
                     /locus_tag="NK767_000989"
     CDS             complement(33694..34359)
                     /locus_tag="NK767_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6693998.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            34335..34508
                     /locus_tag="NK767_000990"
     CDS             34335..34508
                     /locus_tag="NK767_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6693999.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(34736..34942)
                     /locus_tag="NK767_000991"
     CDS             complement(34736..34942)
                     /locus_tag="NK767_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6694000.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(34968..35387)
                     /locus_tag="NK767_000992"
     CDS             complement(34968..35387)
                     /locus_tag="NK767_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCH6694001.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
BASE COUNT        12114 a         6196 c         7633 g         9861 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac taggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccactcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg aaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgacc aataaaatga taaaatcgat cccgcaaaat agaatttttt acattgatta
    17701 agaaactgaa aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg ccagtctttt
    17761 ttttcgtttt tgggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat
    17821 caaatggtgc aaagaaaaaa tcttccattc tattccctcc tttaaaaaag cataacatga
    17881 tttctattta cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt
    17941 cattatgatg tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc
    18001 aaaaatgaaa tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat
    18061 gacaagtcat gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca
    18121 tgggggtacg cttgacccag acgtagacgg tgtgttgccc atttgtattg gaaaagccac
    18181 aaaagttatc gaatatttga cagattctgg gaaaatctat gtaggcgaga taactctagg
    18241 tttcagtaca acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat
    18301 gtctttatct gaaaaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca
    18361 gataccacca atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg
    18421 caataatgaa acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac
    18481 aagtgaattg acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg
    18541 cgggaaagga acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc
    18601 tgcgcacatg tctgatctga cccgaacagc aagtgccggt atgtcccaga aggatgctct
    18661 gacattagaa gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc
    18721 aatagaaaaa ggagtggaac ggttcaaacg agtagatatt actgatgaag tatggcagaa
    18781 agtaaaaaac ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga
    18841 agaaattgct ttgttttata aaggaaaagt tgtcagtatt tatcagccaa acccgaaaga
    18901 aaaaaacaaa ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat
    18961 caagatcaga catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtgct
    19021 gggatttttc gacggcgtgc atatcggaca ccagaaagtc ataaagacag gtatggaaat
    19081 tgcacaaaaa gaaggattga aattagcatt gatgactttc aatcagcatc catctattgt
    19141 ctttaaaaag atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga
    19201 aaaaatggcc gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc
    19261 tcatttagca ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc
    19321 tgtttccggt tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc
    19381 tgaatatgcc aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga
    19441 aaaaatcagc tctacccgta ttcgtggatt actagacagc ggacaagtaa aagaagcagc
    19501 aaaactatta ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg
    19561 cagactacta ggattcccta cagcaaatgt caaagtaaag agcacggttc atttgcctaa
    19621 agaaggtgtt tatgtgtcgg aaataaaaat tggggaaaca tggtatccag ccatgggttc
    19681 aattggacat aatgatacat tcggtgaagg acgggagctg actgtagagt tgtatatttt
    19741 ggactttaat caagatatct atggtgaaca tgtcgcggta cgctggaatc aatttttaag
    19801 agatcaagtg aaattttcta gtgtagagga attgattgaa caactcaagg aggatgaaca
    19861 agcaacagct gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg
    19921 tataactagc aattacaata aaatgggtga taaataagcc caggaaaggg gctgtgacaa
    19981 aagtcttgtc acagcctttt tttccaaata aatggttttt cagaagtagc ttcttcggaa
    20041 ataagccgaa atttcttgaa gctgaccact tctgtcacaa cctctttctt tatagaaata
    20101 aaaggacact agcaattcat gaaaacgatt gctagagcgt atcatttgct ttagactata
    20161 tatcttggtg tacactcact ttaaggcgga aataaaagtt atccgtaata acaggtttag
    20221 gtatgaaaaa ggagtgggta ctatgtacaa ggaattagaa aacaaagtcg cagtagtaac
    20281 tggtggctca aaggggattg gaacagcgat tgctcaacga ttcgggaaag aaaaaatgaa
    20341 tgtagttgtc aactataact cggataaaga aggagcagaa aaagcagtca aagcaatcga
    20401 agaggcaggc ggaaaagcag tctctgtaca agcagatgtc agttctgaag aaggaataca
    20461 gcgtttactt gatacggctt tagaaaattt tgggacactg gacgtttggg ttaataatgc
    20521 tgggatggaa aatcagcatc caacccatga acttagcctt gaagattggg aaagagtgtt
    20581 aaaagtcaat ttgactggcg tttttatcgg aacgaaagcg gctttaaatt actttacaga
    20641 gaataataaa aaaggaaata tcatcaatat gtcttctgtt catgaacaga tcccatggcc
    20701 aacgtttgct cattacgctg cttcaaaagg cggggtaaaa ttatttaccg aaacggtagc
    20761 aatggagtat gcaccgaagg gaatcagaat caatagtata ggccctggtg ctatccgtac
    20821 acctattaat gcggcaaaat ttgatgatcc tgaacaaaaa gaactcttgg aaagtatgat
    20881 cccactagga cgcgtaggtg aaccaaaaga aatagcggcg acagctgcat ggttggcttc
    20941 agacgaatcc agttatgtta caggtattac actatttgca gatggtggga tgactttata
    21001 tccttctttc caaggtggaa aaggataagg gtgaataaaa atgggtttat tgattgcatt
    21061 gatcccggca gttggctggg ggatccagcc actgattttg aaaaagattg gtggcagacc
    21121 aacaaatgag atattaggaa ctggtattgg agcggtgctt gtcggcttac tcgttcaatt
    21181 attcgcttct ccagctggta tttcagtgac tacatttttg attagtttac tttctggtgc
    21241 attttgggtt attggacaaa caggacagta tacatctttc aacttgattg gtgtttctaa
    21301 aacgatgcca atttcgactg cattacagtt agtcggaacc tcgttgattg gtgtcttcgc
    21361 ttttggagaa tggtctggaa gtatggggaa aatcattgga gcagcagcaa tcattcttct
    21421 tgtatttggt tctgctttaa cggcaatcag tgatgatcct ggaaaaaaag gcggtatcac
    21481 aaaaggaatt agtctattag catcaacaag tattggttat tgggtataca gtgcattgcc
    21541 taaactagtt gatgcaagcg gactttccat tttctttccg caaatgatcg gtgtcttttt
    21601 aggggcagtg atttatgtga ttttccgtaa accaaccgca ttttctgaag acaagagttg
    21661 gaaagcaacg atcgttggag ccatctttag tgtgtcagca ttggcttata ttttttcagc
    21721 aaatgaaaat ggggttgcta ctgcatatat cattacacag ctaaacgtag ttatctctac
    21781 attaggcggt atgtacatcc tgcatgaaaa taagacacca aaagaaaaaa aattaacgat
    21841 tatcggatta ttgctgatcg tagtaggaag catggtcacg gtctttattt gaggataaag
    21901 atggtaaaat ttcatttatt aaaaaaatat ttgtttgaac caaacagtaa gtacttataa
    21961 aaaaatagcg ctagaatttc caacatctct ggaatttcta gcgcttttta attgtttaag
    22021 atacaaggga aatatctgtc tggatcaata atttttcatt gatccattga ctaaagttta
    22081 cgacataatt gaagttacca ccgattagta tctccgcatg attgtcttta taaagatcgt
    22141 attgtaaaag acggtctttt ttatttgcgt aaacatcgtt atggtactgg actaattgtt
    22201 ctcggaattg attgattgct gtaaaatcac gatattgaag gttgaaacgc atcgtgattg
    22261 gtgcatttag catgaatcgc tgcatttctt ttgctgcttc ctctgcttga tctccgtata
    22321 ggaactcaaa aagattacgt tcagaaaatt tccattgccc gccaaccttt gttcctttta
    22381 gttttccttc atgaagataa tttctgatag aacgtgtagt aacacctaat tgttcggcta
    22441 attcatttac tgagtaaaaa tctttcaatg ttcaccctcc tatgtgaatg gttctactga
    22501 cttagttgat atttgcttaa tcacgaaatg tagaaagaac gattttaatt aaggtcattg
    22561 taaaaatcaa gtcagaaaat tctaactttt atttctctaa agaaattcgt tctctcctaa
    22621 agtgatatta tatttaaata tacctgaaaa taatgggcag tattatgaaa gatcactgat
    22681 attttctcgt attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt
    22741 ttactaagta gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact
    22801 ccttataaaa atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga
    22861 taggattgaa caaaacctgt ataaggtgat gaatagtttc ctcgccagta aaaggtttat
    22921 tcgtataaag ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga
    22981 aatcaattgt tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt
    23041 atccgtatat acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt
    23101 ttccggtgcg atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg
    23161 gtcagtaaac ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt
    23221 tatcttagca aaaaggtctt tgatcttttg ttgatgttct ttttctttga tttgattgat
    23281 gatcggctga aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc
    23341 agaaaaacaa tagttatgta taaaaaacga acaaaacaca agtaatgttg taaccgctat
    23401 atttatatga taagatgtct caaataataa aatagaaaag agacataaaa atgattgact
    23461 tggcaaagaa attcttacac gaaaatatat attttgagtt gtatcatcat cgagccattt
    23521 atacaaatga agatgctttg gtaataaaga aagaacaagg atttacaggt acagagacca
    23581 aaagcttatt cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga
    23641 aacaaacgga tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat
    23701 cagaacgcat ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg
    23761 aaaaacagat tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg
    23821 caccaggtag accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc
    23881 agctacttga gatgccgata tttttgatgc cggcaaacga aaaaaacaat taaccaagta
    23941 aaatagtgca tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg
    24001 caacgattgg ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg
    24061 aatcagcttt aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt
    24121 ggacgcaaga taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa
    24181 tgatgcaaga tgaaaatatc caaatgatct ttccgacttg gggaggagaa ttattgattg
    24241 agattctcga ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag
    24301 atattagtac gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa
    24361 ctaaccttat tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg
    24421 tactgaaaac aagagaagga gaatcgattc tacagcaatc ttctgaaaag tatcaaaaag
    24481 tgtggcccca tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc
    24541 aatcagtcag cggagatgca gtaaggatga agggacgatt attaggtggt tgtattgata
    24601 cgatcaggca tttaatcggt actccttatg gagatgtcca atcttttaaa agaaagtata
    24661 atgatggaaa gccgatcttg tggtttctag aaaactgtga gctatcgtca gttgatttaa
    24721 gaagatcact tgttcagatg aaactggcag gctggtttga tggcagctcg gggatcatgt
    24781 tcggtagaag tgcggcagag gatttgttag attataaaat gaaggatgta tacgaagatc
    24841 tgtcaaagga attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac
    24901 aactgacatt gattaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg
    24961 ttttgcaaac gtttatttaa aaaatttttt cctgccttgg agtctatcaa agaccaataa
    25021 atctaaatag aaaagaaagt gaaagaagct gacaaaatgc tgtcagcttc tttttgtata
    25081 acatattaga aagtctaatt atctataact gtcacatcga ttaactgttt cacgtcattg
    25141 ggatttcctg taggaatagc tactacagat gcattatcaa tgatttcttg agttgtttct
    25201 ctaatgaacg ctggataaga tgtttgatct gcaggcattt gccaatgtga aggaactgga
    25261 taactagtga ttgtcccgtc atggttactt gaatacacta aaactccctg gtaactgtat
    25321 ttcccagtga gcataaccac atctttcgga tagactgcac tccctgcatc ataaggggcg
    25381 ataggtgttc ctgcaggata atgaataaca tgtaattcga aatcacccga tgtatctcta
    25441 aaagtattac ctctaatagc tagccaaaca cgtgcgtact caatttgtag atcagaatat
    25501 cctgctaacg gatctattga ctgagaagac tgtgaggagg ttgtgtctat tgttgaagaa
    25561 gaaatatttg tctcaactgt actactagat gtagaagaag tattgggctc tgagacttta
    25621 ctgcttgttg aagatgtatt cgtttcagta gaagctgatt tctgagtact tgatttttca
    25681 gctgaactgg tttgtctgat tgaacttttg tctgtggtcg cagatggatc actattttct
    25741 ttactaggag acgtacatcc tacaaggaat aaagagacca acaataaaat aaaacctaac
    25801 tttttcatat aaaatacctc attatttttt taataccagt atagcataat cctgaatttt
    25861 tgaatccgta aaaccttgtc tatgaatata aatagagtgt atcttctttg ataaatgtca
    25921 gaaaaagtgt gacaaataat catcagtaaa attacttgtc acactattga taaggagtat
    25981 ttaattgaag agaatgatta ggagaaagga tctccgtact ttttatagta atcatagaat
    26041 ccaagtttgt atagacgaat cgttttttct cttatttctt ctgtttcaaa atggactgct
    26101 tgaagaatca agtctgtgaa ttctatagga gctgttccat tcgcagtaat caaattacga
    26161 tctttcacag cttgttgttc tataaaatct tctttattct gataattttc cgattctaac
    26221 caaggaaatt gcgcatttcc ggtgtgtttg taaccttgca ataatccgct ctgagctaaa
    26281 taatcaactg ccccacaaat agcagctact ggtacgtctg tttttaacaa tttatcgatt
    26341 atttgatata aatgatgatt ttttatgtcc catgaattgc caccgatcat aataaaaagg
    26401 tcaacttttt caggtatttg ttcgattaag taatcgattt ttgtttttaa tcctccaatg
    26461 gaagtgacct ctgattggat agaagcagtt ttgttttgcc atttctcatt ttggtttagt
    26521 gtgctagtaa tataagcacc ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca
    26581 gttttcattt tcaagctctc ctttactatc tataacaagg acaacagttt taacctacag
    26641 acattcatta aaattgggta gcgacagcga aaacaacaat tcttaagttt tagacagagt
    26701 atacagatag tcaacttaat taaatacgaa ctaaataaaa atgacaggta atcatttttg
    26761 tttaccagca ttataacatt ttgcatacat ggatgacaaa agaaccaatt cccacttgct
    26821 tgagtacaat attaaaagaa actggaaggc aaaaagtaat tatagaaacg aaaaataaat
    26881 catatcgggc actcagacat gaaaacaacc ctagagatat ataactaggt aaccagttta
    26941 atgaaagaaa aaatcatcca agaagtcgat tcttggattt tttagtggat ttgttcgcca
    27001 aatacgttta gtatgcgtat atgagaggtt gtaagcaaag cgttgtggtc tgagcactta
    27061 aggaggaatc tgtgaaaata gcttttcata tttttgcaga ttttgactta atgccgaagg
    27121 tcagctttgc gattaccgtt tatcgagagg ctgtaaaagc accgtgtagc tccgatgaca
    27181 ataagcaagc atttttagag ctaggtttcc ttgattctaa atatgcggct tatttcaatg
    27241 agttggtgct tgaacgcgat tttaagaagg tcgaaaacag gcacgacttt tcgacgtaag
    27301 gagaaagaat ttcaatgata gaaacaataa atcccgcaga aaacaaggtt tctgcgtaca
    27361 ttcatatccc attttgcgag catatctgct attactgtga ttttaataaa gtttttttag
    27421 aagggcagcc agtagatgag tatgtggaaa tgctgctgaa tgaaatgaaa ataatgctag
    27481 aaagtcatcc gacagcgaat ttagaaacac tttacgtagg gggcgggaca ccaacttctt
    27541 tatctgctaa acaacttgat cgactgttat caggtgctag agagatcctg ccttttcaaa
    27601 atggtaatga gtttactgta gaagcgaatc caggtgattt gacccgtgaa aaacttcaag
    27661 taatgaaaaa ctatggcgtc aatcgtttat ccatgggagt gcagacattt gataatcgct
    27721 tattgaaaaa aattggacgt aaacacaccg cagaagatgt gtatcaaact atgcaatttt
    27781 tagaagcaga aaattttacc aatgtaagca tcgatttgat ttatgcatta ccgggacaaa
    27841 cactagaagg atatctggat actttggaaa aagctttggc tttggatctg cctcactatt
    27901 ctttgtactc attgatcctc gaaaacaaaa caatgttcat gaactgggta cgacaaggaa
    27961 gactcgaact gcctgatcag gaaacggaaa ccagaatgtt tgaagagacg attgaagcaa
    28021 tggaaaagca tggacgtcac caatatgaaa taagtaactt tgggctctcc ggacatgaat
    28081 ctcaacataa cctcatgtac tggaacaatg accactattt tgggtttgga gctggtgcta
    28141 gcggttatct tggagatatt cgttaccgca acaaaggacc aatccaacac tatttacgcc
    28201 cattacgagc aaatgaacgc ccggtattgg aagaagaaaa attgacacga aaaaatcaaa
    28261 tagaagaaga aatgttctta ggactaagaa aaaaaacagg gattactaaa aaacatttct
    28321 atgatcgcta tcaaaaaaca attgaatcgg tgtacggtga tgtgctaagg gaattagagc
    28381 aagaagggtt acttgttaat gaagaagacc gtgtttattt gacaccaaga gggacctttt
    28441 tggggaacaa tgtgtttgag cgctttttgt ttgaaaaata gtttaaagaa tatttgtttt
    28501 ttatctgaat ttaaataaaa aaagttcaaa aatacaataa aggactattg aaacgttgat
    28561 ataactggat ttacatcaat tatttagaat tagcactctt gtgtttagag tgctaaaaaa
    28621 tggagtcttt ctcttgacag gagaagggct tcttgctata tttataagtg gattagcact
    28681 tagaatatat gagtgctaat gagaggtgag gaagatgtta acaaagcgac aaaacgacat
    28741 tttacgtctg atcatccaac actatacgag tagtggcgtg cccgttggtt ccaaaacatt
    28801 gatgtctgag ggagtcaagg ccagctcagc aacgattcgc aatgatatga aagcattaga
    28861 agatgaagga ttgctgttga agactcactc ttcttctggg cggattccat ctgctttagg
    28921 ctatcgttat tacgtggatc atttgttaaa acctgctcgc gtagcagaag atgatttgct
    28981 gctgattcgt cagtctctag gcagagaatt tcatgaaatc aatgagatta ttaagcaatc
    29041 tgctgagata ttatctgagc tgacaagcta tacgactttt tcattgggac ctgaaataaa
    29101 agaccgccga ctgactggct ttcgtatcgt acccttgaat aatcgacaga ttattgctat
    29161 cgtcgtaaca gatatgggga atgtagaaag ccaagtgttc actcttcccg aatatatggg
    29221 aagtcaggaa ctagaaaaaa tggtacagat tgtaaacgac cgtctagtcg gagatcctct
    29281 gatgacggtg taccataaac ttcgtacgga gattccgatg attcttcata aatatttcca
    29341 aacgacagaa gggatgtctc agttatttga caccgttctt ggtcatgttt ttgaagataa
    29401 ggtttatgtc agtggacaaa tgaatctctt ggattatgag ccccgtcaag atcttgatca
    29461 gttcaaatcg atgttttctt ttatgaaaga ctcagacgga cttacacaaa tgatcactcc
    29521 gatggatagc aatattcata tcaagattgg tgctgaatta ggcaatgatt tattgcagaa
    29581 tatgagtatg attcaagcaa gttacgagat agatggtcat ggaagaggta caatagcctt
    29641 gttagggcct actagtatgc cttattctcg gatgttcggt ctagtcgatg tttatcgaag
    29701 agaactagca gctaaacttg ctgattacta ccgttcgctt gacttgtcaa actcgtaatg
    29761 tgaataacga agctatttgc agctgatcgt tctctctttc ttagtaagag caatcaaaca
    29821 gctgactgaa taatgaaagg aagactgtat cgtgaaagaa aatcaagaag aactagataa
    29881 agaaatgaca gatgctcagc cagaaccgga aatcgatgtc gaagctgcag aagattcggg
    29941 tatttcagaa gctgaagcgg aagaattcga aacagcgaaa ttaaaagctg agttagaaga
    30001 aatggaagat aaatacttgc gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa
    30061 aaatgagcgt gagcaattac aaaaataccg ttcacaagat ttagccaaaa aattacttcc
    30121 atcaattgac aacctggaac gtgcattagc cacagaagtg tcagatgatc aaggggcagg
    30181 tttgaaaaaa ggcgtggaaa tggtcttgga aagtttgcgt aacgcactgg aagaagaagg
    30241 aattgaaaaa atccctgcta aaggagaagc ctttgatcct aatcttcatc aagcagtaca
    30301 aactgttcca gcgactgaag atacaccagc cgatacgatt gtagaagtac tacaagaagg
    30361 ctacaaatta catgatcgcg tgttaagacc aaccatggtc attgttgcac aataattgtg
    30421 cggatataaa acttacactt tcaattaaag aaggagacgt taattatgag taaaattatc
    30481 ggtattgact taggtacgac taactctgca gtagcagtat tggaaggaaa cgaagcaaaa
    30541 atcatcgcta acccagaagg aaaccgtaca acaccttctg ttgtatcatt caaaaatgga
    30601 gaaatccaag taggggaagt tgcaaaacgt caagctgtga caaacccaaa tacgatttca
    30661 tcaatcaaac gccatatcgg tgaagcaggt tacaaagtag atgtagaagg taaatcatat
    30721 acaccacaag aaatctcagc aatgatcctt caatatatca aaggattcgc agaagactat
    30781 ttaggtgaaa aagtcgaaaa agcagttatc actgtgccag cttactttaa tgatgcacaa
    30841 cgtcaagcaa caaaagatgc tggtaagatc gctggtctag aagtagaacg tatcgtcaat
    30901 gaaccaactg cagctgctct tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc
    30961 ttagtattcg accttggtgg cggtactttt gacgtatcta tccttgaact aggagacggt
    31021 gtctttgatg tattgtcaac agctggcgac aaccatctag gtggggacga ctttgataac
    31081 aaaatcatcg actacatggt agcagaattc aaaaaagaaa acagcattga cttgtctcaa
    31141 gacaaaatgg ctttacaacg cttgaaagac gctgctgaaa aagcgaaaaa agatttatct
    31201 ggcgtatcaa gcacacaaat cagcttgcca tttatcacag caggagaatc tggaccattg
    31261 catttggaaa tgacattgac acgtgcaaaa tttgatgaat taacagctga cttagtagaa
    31321 cgtacaaaag taccagtacg tcaagcgcta aaagatgcag gtttatcaca atcagaaatc
    31381 gacgaagtta ttttagtcgg tggatctact cgtatccctg cagttgtaga agcagtacgt
    31441 aaagaaacag gaaaagaacc aaacaaatca gttaaccctg atgaagtagt tgctatgggt
    31501 gctgctatcc aaggtggcgt gatcactggt gatgtgaaag atgttgtttt acttgacgtt
    31561 acaccattgt cattaggtat cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc
    31621 aatacaacga ttccaacaag taaatcacaa gtattctcaa ctgcggcaga taatcaacca
    31681 gcagtagaca tccatgtatt acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta
    31741 ggaagattcc aattgacaga tattccagca gctcctcgtg gtattccaca aatcgaagta
    31801 acattcgata ttgataaaaa cgggattgta aatgtatctg cgaaagatct aggtacacaa
    31861 aaagaacaaa aaatcacgat caaatcttct tcaggtctaa cagacgaaga aatcgaacgt
    31921 atggtaaaag atgcagaagc aaatgcagaa gcagataaag ctcgtaaaga ggaagttgac
    31981 ctacgtaatg atgtagatgc attgctattc tctgtggaca aaacattgaa agaattagaa
    32041 ggcaaagtgg acgaagaaga agtgaaaaaa gcagaaacag ctcgcgatga attgaaagcc
    32101 gctgttgaag caaacaacat cgaagaaatg aaaactaaac gcgatgcatt gaacgaaatc
    32161 gttcaaaact tgactgttaa actatacgaa caagcggcac aacaacaagc tcaagaaaac
    32221 ccagaagcag ctcaaggcgg agcagatgat gtcgttgatg ctgattttga agaagtcgat
    32281 ggcgacgata aataatcagt tcactgaatt tcgagagaag gccaaagttg ctactttggc
    32341 tttttctttt gaaaaatagt ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga
    32401 taacaagcga cgtgaaaatg atgagaacca ataggaggaa agcctatggc aacaaaacgt
    32461 gattattatg aagtcttagg gctctcaaaa ggagcatcag aagatgaaat aaagaaagct
    32521 tatcgtaagc tttcgaaaaa ataccatcct gacatcaata aagaacctga tgcggaagaa
    32581 aaattcaaag aagtatcaga agcatatgag attttaagtg acccgcaaaa acgcgctgca
    32641 tatgaccaat acgggcatgc aggcacagat ccaaattacg gtgctggtgg agctggtggc
    32701 ggatttggtg gattcggcgg cttctctggt ggcggcttcg gtggttttga agatatcttc
    32761 gactctttct ttggaggagg cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct
    32821 gatttgcaat atacggtcga cttgtctttt gaagaagcga tttttggtgt agaaaaagaa
    32881 atcaagtata atcgggaaga aatctgtcat acttgtggtg ggaacggtgc aaaaccaggc
    32941 acacaaccaa ctacttgtca taaatgtcat ggtgcaggta cgattaatgt agaacgccaa
    33001 acaccacttg gacgagtgat gagccgtcaa acttgtgatg tttgtcatgg gacagggaaa
    33061 gaaatcaaag aaccttgtcc aacctgtcat ggctcaggac atgagaagaa agcacattca
    33121 gttaaagtga atgtaccagc tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa
    33181 ggagaagctg gagaaaacgg cggaccattt ggtgatttgt atgtggtctt ccgtgtagaa
    33241 gaaagtgata tctttgatcg tgaaggttct gaaatttact atgaattgcc tttaagtttt
    33301 gtacaagcag ctttggggga tgaagtccaa gtaccaactg tccatggaga tgtgaaattg
    33361 aagatccctg ctggaacaca gacaggtacg aacttccgtt tacgtggaaa aggcgctccg
    33421 aagttacgtg gtggtgcaac aggcgaccaa catgtcaaag taaaactgat cacaccgaaa
    33481 aatctaaatg aacaacaaag agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa
    33541 gttgaagaac agcaagaaga cggtttcttc gataagttta aagatgcttt tggcgggaaa
    33601 aagcggaagt agtttttcta gtaaaaacgc taagtcatta ataattaaaa accagacgaa
    33661 caatcatatg ttcgcctggt ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag
    33721 ctttagaata tgataatcgt catagtttct aggggtataa ccagtgagag aatggaactt
    33781 gttcaatcga tattgcaagg tgtttttatg aataaataat tcttctgagc aatttttgat
    33841 gcttccgtta tttttactga agcatgccaa agtcttcttg aacaaatgta cttcttcttc
    33901 tgtcaggctt ttcaaaatac gttttacata taatgttcgt tgatctgacg ggattgctgg
    33961 taaaacgatc ccgaggtcta aatcttcaaa aaagcggatc tcgttcacag attgatggaa
    34021 agctatccat tcgcaaactt gtttggcatg aaggaaagat tgcggtgtct cttgctctgt
    34081 ataacaaagc gacccaattc caaagtttag ctgtaactga tacattttat taaactttgt
    34141 ctggatttta tcaagcaatg atagcaatga ggctctggaa atccgattga ctaggataat
    34201 gatttgttgt ctatgtacag tgagcaaagc ctgtccgtaa taagagaaga ccctttcgag
    34261 caactccaat aaaggattga tccgttcttg acacaattgc tgattgtcag aaagttgtcc
    34321 cacaactaca taatatggag aagaaaaatc aagctcaatt aagtgagggt ggtatcgact
    34381 atgtacttga tatcatcaat gaaccgatgg tactttctta tgcgatggaa caggtagagc
    34441 gattgcttgt ttgtgcagga aaacaagcga tgcggctgat acgattaggt gagaaaataa
    34501 gaagataaaa atacacggtc ccttcatggc gaccgtgcat ttttggtttt tattaaattt
    34561 tatatctaat tttcaattat ccgcataaac actatacatt caagcatttt gaagtataga
    34621 aaaacactca tcttaccacg aatttaccac gattgaatga gccttgatat gaccgtaaaa
    34681 ctactaaggg agataacacg aaactgtgta tctcccgatt tttttataca gttgattact
    34741 tcactccgat ttcaaatttc aatattgctt tcatcatgtc tgtatgtatt tctcctttta
    34801 gttcttcatc aacctgcata atgattttac cactttcaga gtggtaaaaa ggacgcaagc
    34861 ataatttaga aatataaccg tcataaaact tcaaaatctc tttaactgcc atttcatctc
    34921 cgcttgacgc tttgcaaatc aattcaaaag agggatagcc ataatcttta cttcctttcg
    34981 ctcaaaagca tttttaactt ccttagtcct ctagttcttc tactgcaaat agcagaacgt
    35041 gatacatggt acagttctga aatttctctg tcgttcattc cttgaaaata acgtaataaa
    35101 atcgcactac gctgtttttc tgatagtcta tgtaaaactg taccaagtgt ttcatcagat
    35161 acataaattg taaagtgcaa gactttaaat gatacacaat cataaaaaca ggtatcaatc
    35221 gttccacgtt caacttgttt cgtttcggct atttcacaaa acaatacttc acgtttagaa
    35281 tgtcttgcaa gctgtctgtt tcgacttttc actttgttct taatgacaat cagcattaaa
    35341 gaattgaact gtaatctgac gatatgctcg aaagaagatg tcctcataat ctcaccccct
    35401 ttctgtctag gaaaggaagt gagccttgct cgtttatctc ctttcactct tagtcccaat
    35461 gtgaaagggg gatttgttgc attactgata aataaacttt gtaaaaaagt tctgaatagc
    35521 caaaaaagca tataaacaga tttatttctc tgtttacatg cttctgttat tctatctata
    35581 tgatttataa aaccacattg gtggacgtac ttatctattg cagatagacg actttttttg
    35641 acaagaaccc aatgtaagga aatttattgt atatgatgta cttcatggcg acgttgacct
    35701 ccaacaaacc gccatttgga agtaatatac aatattttaa cagcgtaaat agcactacca
    35761 tataacggtt ttttttattg gcgtttagta gtgcttttta ttaa
//
LOCUS       DAHUVV010000020        34636 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.020, whole genome shotgun sequence.
ACCESSION   DAHUVV010000020 DAHUVV010000000
VERSION     DAHUVV010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.020"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(240..2132)
                     /locus_tag="NK767_000993"
     CDS             complement(240..2132)
                     /locus_tag="NK767_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6694002.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2129..3856)
                     /locus_tag="NK767_000994"
     CDS             complement(2129..3856)
                     /locus_tag="NK767_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6694003.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3872..4522)
                     /locus_tag="NK767_000995"
     CDS             complement(3872..4522)
                     /locus_tag="NK767_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCH6694004.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4726..5022
                     /locus_tag="NK767_000996"
     CDS             4726..5022
                     /locus_tag="NK767_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCH6694005.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5194..5814
                     /locus_tag="NK767_000997"
     CDS             5194..5814
                     /locus_tag="NK767_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCH6694006.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5906..7072)
                     /locus_tag="NK767_000998"
     CDS             complement(5906..7072)
                     /locus_tag="NK767_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCH6694007.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7084..7437)
                     /locus_tag="NK767_000999"
     CDS             complement(7084..7437)
                     /locus_tag="NK767_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCH6694008.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7565..8587
                     /locus_tag="NK767_001000"
     CDS             7565..8587
                     /locus_tag="NK767_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCH6694009.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8606..8767
                     /locus_tag="NK767_001001"
     CDS             8606..8767
                     /locus_tag="NK767_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694010.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9026..9460)
                     /locus_tag="NK767_001002"
     CDS             complement(9026..9460)
                     /locus_tag="NK767_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694011.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9570..11429)
                     /locus_tag="NK767_001003"
     CDS             complement(9570..11429)
                     /locus_tag="NK767_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCH6694012.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11547..12707)
                     /locus_tag="NK767_001004"
     CDS             complement(11547..12707)
                     /locus_tag="NK767_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HCH6694013.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13014..14438)
                     /gene="araA"
                     /locus_tag="NK767_001005"
     CDS             complement(13014..14438)
                     /gene="araA"
                     /locus_tag="NK767_001005"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCH6694014.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14454..15152)
                     /locus_tag="NK767_001006"
     CDS             complement(14454..15152)
                     /locus_tag="NK767_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCH6694015.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15109..16770)
                     /locus_tag="NK767_001007"
     CDS             complement(15109..16770)
                     /locus_tag="NK767_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HCH6694016.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16988..18388
                     /locus_tag="NK767_001008"
     CDS             16988..18388
                     /locus_tag="NK767_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCH6694017.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18640..19719)
                     /locus_tag="NK767_001009"
     CDS             complement(18640..19719)
                     /locus_tag="NK767_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCH6694018.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19805..20764)
                     /locus_tag="NK767_001010"
     CDS             complement(19805..20764)
                     /locus_tag="NK767_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCH6694019.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20776..22287)
                     /locus_tag="NK767_001011"
     CDS             complement(20776..22287)
                     /locus_tag="NK767_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCH6694020.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22309..22872)
                     /locus_tag="NK767_001012"
     CDS             complement(22309..22872)
                     /locus_tag="NK767_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCH6694021.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22878..23741)
                     /locus_tag="NK767_001013"
     CDS             complement(22878..23741)
                     /locus_tag="NK767_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6694022.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23755..24651)
                     /locus_tag="NK767_001014"
     CDS             complement(23755..24651)
                     /locus_tag="NK767_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6694023.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24826..26115)
                     /locus_tag="NK767_001015"
     CDS             complement(24826..26115)
                     /locus_tag="NK767_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCH6694024.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26087..27604)
                     /locus_tag="NK767_001016"
     CDS             complement(26087..27604)
                     /locus_tag="NK767_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCH6694025.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27802..28692
                     /locus_tag="NK767_001017"
     CDS             27802..28692
                     /locus_tag="NK767_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCH6694026.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28765..29589)
                     /gene="nadE"
                     /locus_tag="NK767_001018"
     CDS             complement(28765..29589)
                     /gene="nadE"
                     /locus_tag="NK767_001018"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCH6694027.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29601..31073)
                     /locus_tag="NK767_001019"
     CDS             complement(29601..31073)
                     /locus_tag="NK767_001019"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCH6694028.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31710..32072)
                     /locus_tag="NK767_001020"
     CDS             complement(31710..32072)
                     /locus_tag="NK767_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCH6694029.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32295..33047
                     /locus_tag="NK767_001021"
     CDS             32295..33047
                     /locus_tag="NK767_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCH6694030.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33187..34110)
                     /locus_tag="NK767_001022"
     CDS             complement(33187..34110)
                     /locus_tag="NK767_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6694031.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34125..34397)
                     /locus_tag="NK767_001023"
     CDS             complement(34125..34397)
                     /locus_tag="NK767_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694032.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10233 a         7205 c         5956 g        11242 t
ORIGIN      
        1 gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttaag gaagaggttg
       61 taaaatcagc ggtttgacct gagtattaga gatacaaaac ggaaaaatag cttctcatat
      121 tttgccgttt tttgagctaa taccgaagga cagctgattg aacaccgttt attcggaaaa
      181 agggactgtg acacgacttt tgtcacagcc cctaaaaaaa ataataaatt tgtactattc
      241 tatgaagctt cttcttcaga aaactgactg ttataaagat ctgcatagaa tccgtctttt
      301 tccatcaatg tatcgtggtt tcctgtttct acgatcgaac catgattcat cacaataatg
      361 ttgtctgcat cgcgtattgt agacaagcgg tgagcaacaa cgaaacttgt ccggttttcc
      421 aataggcggt tcatcgcttt ttgaatcagt atttctgttc gtgtatctac actagatgtt
      481 gcttcatcaa ggatcaggac atctggattt gctaagaatg cccgcgcaat tgtgattagc
      541 tgacgctgcc cttgcgaaat attgcttgca tcctcgttta atacagtgtt atatccttct
      601 ggcaatttac gaacaaaatc gtctacgtga gcagctttag cagcctcaat gatttgttcg
      661 tctgttgcat cgtcgttccc atatttgata ttgtcataga tacttcctgt aaacagccaa
      721 gtatcttgca gtaccattga aaactgtgcg cgcaattcat cacgtgaaag atcacgggta
      781 tctacaccat cgtaacgaat actacctcca gaaacatcgt agaaccgttc aagaaggttg
      841 atcagtgtcg tcttccctgc accagtcgga cctacaatag cgaccatctc accaggtttg
      901 acatccaagt taaaatcagt cattagcaag ttatctggtg tgtagccgaa ttgaacattt
      961 tcaaaacgaa ctttgtatgg tgtatctttt tcttctggaa ggccagattt ttcattggac
     1021 atctcttctt catccaacac ttcaaatacc cgttcggctg aagcaactgt tgattggata
     1081 gtgttcatca aacttgcaag ttgagtgatt ggttgagaga actgtgttgt atattgcaag
     1141 aacgcttgaa catcccctaa tgtcatgttc ccatttgcaa cttttactcc gcctagtaca
     1201 gcgacgaaca catagcctag atttttaacg aaattcatca acggcatgat gattgccgaa
     1261 ataaattgag ctttccaacc agcggcatac agattctgat tttctttttc aaatacttcc
     1321 tggtcttttt cagtatgatt gaatgtttta acaatggtat gaccaccata tgtttcttct
     1381 acttggttat tcaataaacc taaacttttt tgctgatctg cgaaaaacac ttgtgatttc
     1441 ggcgctacga tcatcacgat gatcaagctt aaagggatca tcaataacgc gactaacgtc
     1501 aattgccagc tgattgaaag catcatcacg atgaccccaa tcaatgtgac tgcactagtt
     1561 acaaattgag tcaaattttg ctgcagcgtg cttgcgatgt tatccatatc attgattgct
     1621 cgagacatga tatccccatt tgtatgtgta tcataatagg aaatcggtac acgattcatt
     1681 ttggcttcca aatcatgacg taattcataa actgttcgtt gggaaacacg tgtcataata
     1741 aattgctgta aaaagttgaa tacagcagaa atcaaataca tcacgattac tgtcgcaatg
     1801 atctgcgcga ttttatcaaa gtctatcgga aattttgtta cttgttggcc ttgttgcatc
     1861 tgttgagcgc cagccattac acctttgaat atttctgtgg ttgctttccc caagaccttt
     1921 ggtgtttgga tttggaagat cacggctgct attgctagta caaatacagc gatgatcgca
     1981 accatacgtt tagacatata gccaaacaat cggcgtaccg ttccccagaa atttttaggc
     2041 ttttcacctt ttacaccgag atttctgcca ggtccaccat gtcctggacc gcctgctggt
     2101 ccatgagagg gacgtgtgtt tgttttactc atgctaagtc ctcctctctt agttgtgaat
     2161 gaacgatttc ttgataagtt tcgtttgttg ctaatagttc ttcatgtgtt cctttgccaa
     2221 ccagttttcc ttcatctagc acaaggatca tatctgcatc cattactgta cttactcgtt
     2281 gagcaactaa aacggtgata ctttctttca tattcgtttt caatgcttgg cgaagattgg
     2341 catccgtttt gaaatccaac gctgagaagg aatcatcaaa cacatagata tctgctgttt
     2401 tgactaatgc ccgtgcaatt gccagacgct gtctttgtcc accagagaag tttcctcctc
     2461 cttgttccac atggctatct aagccatctt ccatctctga aacaaaatct ttagcttgtg
     2521 cgatttctaa tgctttccaa atcatttcat ctgttgcatc cggcttacca tactgcatat
     2581 tgtctcggat cgttccagag aacagaactg ctttttgagg aacaaagccc attaattctc
     2641 gcaaattata ttgagtcatg tcactaatgt ccgttccatt tatttggatc gaaccagatt
     2701 cgatgtcata taatcgaggg atcaaattga ctaaggtcgt tttacctgaa ccagtccccc
     2761 ctatgattgc gacgatctcg cccgacttgg cttggaaatc aatatcttcc aatgcaagtt
     2821 tttctgctcc atgatatcga taattgacat gattgaaggc caatgttgcg ttttttccag
     2881 caaaagaaac tgttttagga ttttcagggt catggatacc gattttttca tccaacactt
     2941 cattaatacg gtcagctgaa gcttgtgcac gaggcaccat gataaaaata gcggaaagca
     3001 tcataaagct gatcaagatc tgcatcgcat aagtcataaa tgcaatcaga ttaccgactt
     3061 ccaaagtcat atctgctatg tagtgtccac caaaccaagt gattgctacg tttgtaccgc
     3121 tcatgatcaa tgtcatcatt ggcatcatca atgcgacgat cgtattaact ttgattgccg
     3181 tatttgtata atctttattc gctaaatcaa aacgattttc ttcaaagcgt gtcttatcga
     3241 atgctcgaat cactctgacc cctgtcaatc cttctcgaaa taccaggttt aaacggtcag
     3301 ttttcttttg catacttttg aataatggaa cagcaaagta catgattccg ccgatcaaaa
     3361 taatcattac tgggatgaca actaagaaga tttttgtcaa ctgatgatct ttttgataag
     3421 caagtatgct cgcgccaatc aacgtgattg gtgcattgat catcatacgt aaaaacattt
     3481 gtgtaaccat ttggatttga gtcacatcat tggtcgttct tgtgatcaaa gaggccgttc
     3541 catatttatc aaacgcatct tttgtcagac tctctgattt tttatagaca tccgtacgca
     3601 attgcttacc cagtttctgt gattcacgag tagcaaaaaa tgtatttgcg atagcagcta
     3661 agatactgat caaagaaaag ccgatcatca caaaacctgt atgccaaata taatcgacat
     3721 ctccttgcgc taccccttta tcaataatat tggaggtaag tgtcggcaaa tataaatctg
     3781 ccagtacttg gatcaccatg aaaactgcag ctgcaattgc tgaagtcaag gagatccgtt
     3841 ttacgatttt tagcatctag ttcccttctt tctatttata tactccgttt ttcagccaag
     3901 aaagtttcaa attgaattca ctgattgctt gctctactga gtaatcttca gatgttgaaa
     3961 ataagcgata cgaatgcgca attgttgtaa atacagtatt catcactaat tggatcagta
     4021 attccaattc atgatcattt tttactttca gcttcgtccg atcgatcaat tcaagcaact
     4081 cttttgcatc ttgtttgtgt tctttatgtg gatcgtgagg cgttttttgg cgactcgtat
     4141 gaggagcgac ttttcttgag gcacggtaat ccatgttcat aaacaagtta cgataaaaag
     4201 aagcattctc gccttccaaa atctcataaa tcattttaga aaaataggat tcgaatcctt
     4261 tgaatagatc accatcggat tctttcatta gttgtaacat atctttagag ctgtcacgtc
     4321 tcattgtctc aaagtaataa aaataaagat cctctttatc ttcaaaatac tgatagaaac
     4381 tgcctctagg gatatccgct aatttgacga tattagcaat cgaagcttct tttaagggta
     4441 ctctagaaaa ctcgattctc gcagcctcga ttagtctttt ttgtttttct ttaggcaaat
     4501 ggaaaaatgt ctgtgtcggc atatggatcc tcctttcttt caagatgaca cattgtcata
     4561 tgacgctgtg tcattataac acctcctacc tgttttgcaa gacatttgca tagttttttt
     4621 cattatcatc cgatacatta aaaaagtgat aagaggaatc cttccatttg aattaatgag
     4681 agttgcccat taaaatagcc acatactaaa caaaggatga gataaatggc ccaatcaatt
     4741 agtgtccaag acttcaataa attgcctctc gatgctgaca gcatcgtttt agatacaaga
     4801 aataccttag attacacaaa aaaccactta agccactcgc tttctatccc cgctgctatc
     4861 ttgccaagac aattaaaaaa tctagatcca aagaaacttt attatattct aagctataca
     4921 ggcaggcgat cagaagcatt agccgatcaa cttactgcta atggttttcg tgctgtttat
     4981 atcattggtg ggatggaagc tcttcagtcg agtgttgctt gaatattgct tgttagattc
     5041 aaaagaggct gtgacaaaaa atcgtgtcac agcctctttt ataccctgtc tttttttagt
     5101 tatttattct tcaaccttat caatatgact tgatagcctt ttctttttct catagttata
     5161 acaatcatag caataaaagg agaaggtaag catatggcat ctgttttagt agtcaaaggt
     5221 catcccctaa ctgcagaaga atcgcgcaca gttaaggcat tgacgtcttt tttaacaagt
     5281 tataaagaaa atcatccaga tgatgaagtg actgttttgg aactttatcg cgatgatatt
     5341 ccggaaatcg atgaagaatt attatcaggt tgggaagcat tacgtgctgg tgcagaattc
     5401 acttccttat cagaaagtca acaacaaaaa attgctcgtt tcaatgaatt gactgatcag
     5461 ttccttgctg ctgataaaat cgttatcgca aacgccttat ggaatctaaa tatcccaaca
     5521 aaattaaaag cttggttcga taccgtcaat gtagctggaa aaacgttccg ctatacagaa
     5581 aacgggccag aaggattagt gacaggtaaa aaagcactgc acatccaatc taatggcggt
     5641 gtatacaacg gacaagactt tgcctctcaa tatgtaaaag gtattttaaa ctttgtaggc
     5701 atcgatcaag tagatcagct attcatcgaa ggaatcgatt atgaccctga ccgtgcagat
     5761 gagttgatgc aaaatgcatt agataaagct gctgctttag gtaaatcatt ttaaaaattg
     5821 agataaatgc atacaaaaga gacagaggaa aaaatcttca aagcagcatt tgtaccttgg
     5881 atattttccc atctgtctct ttttgttatt ctttctgctt tttcaacgga ttttcttcat
     5941 aaagagaaga gattgttgtg taccattcaa aaacatgagt aatcactact ttagctgctg
     6001 cataaacagg aatccccaag atcacaccga tgattccgaa caatttccca gaagtcagca
     6061 gaactaataa aatcgttact ggatggattg ctaactgatt ccctagtact aatggagaga
     6121 tcaagcgtcc ttctattgtc tgctctactg caaaaacgat cagtactttg atgatcatga
     6181 atggacctgc tacaatggct aaaaagatag ccggaatcat cgctaaaaat gatcctaagt
     6241 aagggatcag gttcaaaaaa cccgcaataa tccccaacgt gacagcatac tctaaaccaa
     6301 tgaccgcaaa accaatcata aacatgattg ccacagcaaa agccacagtt agttgtcctc
     6361 ttatataaga agaaacttga ctattcattt cactcagcac attcaacgtc ggtttttgca
     6421 tgcgtgtcgg taaaaatttg acaaaataag gtgctaattg tttaccgtct ttcaatagat
     6481 aaaataagat aaacggcatc gtcagcagag ccactaaaat agtcgctacc gcgccgacaa
     6541 aacttccaag actctgaact gtactcttag atatatcttg gatcatatcg cctgccgaat
     6601 tcatgaactt atcacctgct gtttccaact gttcgcgaaa ttggttgaac agcgggtccc
     6661 gcaaaatctc ggttaattta ttatcaatcg tatctacata ttgagggaag ttactgatga
     6721 agctcactgt ttgttcctga atcttaggga tgatcactac gctcccccat gcgattagtg
     6781 caacaaccaa aacaaacagc ccaatgatac tgtaaagcct tggtactttt cgcttttcaa
     6841 agtaatcaac taccggattc atcaagtaat acaagattcc tgctaaaatg ataggcagac
     6901 ctacgattgc taaaaattgc cagattggtg caaacaaata tgaaactttt gtaaataaaa
     6961 agacgatcag caatattaac aatacaacaa gtaaggctgt gactacttga ttatttaaaa
     7021 accagcgcca aaaccacgat accggtctgt tcttcttatt ttttccttcc ataattcctc
     7081 ctgttagtta taaaaaattt ggcttcccaa accaatagca ggcagtctat gcggatgaag
     7141 atagattcca tttgcaattt gtccttcttc tggacaagca tcaaaaatca atgatacgtg
     7201 agctactgtc tctaaattcc cattggccat cggaccgaca taatcgatcg tatatacctg
     7261 atcatcaaaa gcaattgttc cgcctgcttt caaagaaaaa ggttcgtctt ttgatacttc
     7321 ttgaatcacc gaatattttc gtaaggcagc tgttgctgtt ttatcgaaca aaatcagcat
     7381 gggttcttcc ttgtttattg cttctgtccc aatttctgta atctttgcct gaatcattgc
     7441 actctctcct ttaattgctt tcaaaaacag tataacacat tctcattctt tagccgtgtg
     7501 gaataattat gtgatagaat aaaaacgaaa actatttcat tacaattagg aggaatcagc
     7561 tcatatgatc gaaaagataa ttttaggaac ctatacgcgt cgcgaaagta aaggaatcta
     7621 tacgatcgac cttgatacag aaaaagaaga attagccaac ctttcattag ctgttgaaga
     7681 aaatagccct acctatgtag caaaaagtaa agctggcaat ctttacacag ttactagtgt
     7741 ggatggatta ggtggtgccg gtgcgtacga taaggatttt cattttttga atgcggtaac
     7801 agagtctggc gctcctttgt gttatgtagc ggttgatgaa gcacgtcaat tactatacgg
     7861 ggcgaactat cataaaggtg aagtaaatgt ctaccatata ttaggagatg gcgaactgaa
     7921 agcagcagat tcaatattcc atcaagaagc gacaggtcct cataaaaacc aagatcatgc
     7981 gcatgtccac tatacggatc tgactcctga ccaacgatta gtcgtatgtg atcttggtac
     8041 agatcgtgtg tatacttatg atgtgtccga aaacggcaaa ttgaatcttg taactacctt
     8101 cactgcagag ccaggaacag gccctcgcca tcttgtcttt catccaaatg gacaattcgc
     8161 ttatttattt ggtgaattag acagcacagt ccgcgtgctt tcttataata aagaaacagg
     8221 tgcttttgaa gaattacaga aaatatcgac cttgccagaa gaattcgacg gtgaaaatgg
     8281 aggggcagct atccgcattt caaatgatgg caagttcctt tatgcttcta accgaggaca
     8341 taactcgatt gctgtattcg cagtttttga agatggttcc cacattcaaa acaagcaaat
     8401 catctcaaca gaaggggatt ttccccgcga tttcgcacta gatccaagca ataattttgt
     8461 cgtgtgtgcc aatcagaaca ccgataatct gacattatat cgccgtcatc ctgaaacagg
     8521 attactggaa ttactgaaaa aagatgtcta tgtaccagaa tgtgtctgtg tttattttga
     8581 tcgttagtaa gaaaaggaga atgaaatgaa tcagcaagac gtattagaca gactacggga
     8641 agaactaaat attcctttct ttgatgggaa aatcgaagat aaagagtata gcgaagagga
     8701 atatcagaaa ctcaaagccg atttacagaa ctactttgaa caatatgtac agaatgtaga
     8761 aaactaatca gctttccata ctatctgaaa agaacatcag gacaagactt ttccgtctat
     8821 cctgatgttc ttttctacaa ataaaaaacc gccgagatgg ttagactcga cggaaaagga
     8881 gtatttactc gattttgagt aagtacaagg ttgttgttgg ttataggtat gtcattatca
     8941 taaaggattt tgatcactct atcataaagg taatattaca tttatattag ataactctag
     9001 tttttattgt tatcaaagat ttcttttatt ttttcaacaa caaattagtc attttttgga
     9061 tggcagctgt caattggtct gtcgcttggt cttgttttgc ttcagatgta ttgaatagat
     9121 cttcatctac ccctgattca agatcgatac tttggatcat ttcttcacag cctgctacat
     9181 atgaaagata agctttttca aaatttttat gaatgcccat tgcttttgct ggtggacgca
     9241 aagtactgac tttcttaagc attgcccgat atttttttgt tccttcttca aataacagga
     9301 tgatctcttc aagagtcgat ttgtccagtt cacttgtttt tccttcatca attgcgtttc
     9361 tgatcacttc atatttcgga tgcattcctt ctccgattgc ttctgtttcc ttgacgattt
     9421 gatcgatgga ctgcacataa tagccaatat tcggtaacat gttcgttttc ctactttctc
     9481 cattgttgtt attatttatt ctacttgttt ttatcctcaa atgacaagta cttgaaaaaa
     9541 tttaatggtt tctatacagc tcgccattct tattcgtgct caaagaaacc atctgctaaa
     9601 ctatattgtt tccctgcgac aatgtcatat tggatcaatt gatattcgtc gtaaatctct
     9661 tgttgcgagc gattgaattg cgcttcttta taccactgtc cgttttgata gtacagttct
     9721 cgttcaaacg ccggaagttc ctgttctaat gataaaagca gctgataaaa gccatttgtt
     9781 gtctgattcg tctgttccat tagattcgct gcaaaataaa acggactggt gattgcatcg
     9841 tgagtcgttt gcttttctaa ttctccttta ctgtcgaaca taagaaattg tgtttcatgc
     9901 aatgtttgtt tgtcattttt tgcttggatc gtatctgaat agatccctgg aagatgatcc
     9961 ccccaaaaaa caactaaagt ccttctagaa agattcttca attcttccgt gaactgcttc
    10021 agtgccgtgc tgctatagga aatatcttgc agataattct ctaagtcaag tgtcccactt
    10081 ccatctgaag tttttgcagg atagtctaga ttatcatact ttccatcgta gggcatatgt
    10141 gtctgcatcg tgactaaatg aatgaactga ggtgtttttt cttccttgag aagtgtcatt
    10201 acttctttgt aagctgactc atcagaaata tatggattat tctctatcgt atccgtatag
    10261 gtcatcgtac gctcgctgat gaactggtca aatcctaatg tctgataaac atcctctctt
    10321 ttatacatcg aagtattgta tggatggatc gctgtcgtct ggtatgattg tgcgtttaac
    10381 gctgaaacga tagaaggaag ctgatccatt tttggtacaa gcatcgtgta aggggtggtg
    10441 agttgcgcat taaataacgc catagaaaaa ccagtcaatg cttcaaattc gatatttgct
    10501 gttccaccgc catagttttg agaaagcata ttgccactat atgtttgatc agctacttca
    10561 taataatctg ccaatggctc accagatact tctacgccgt ttaatcgact aggatctgag
    10621 aaactttcac tcatcacgaa cacgatgttc ggcgactctt cctctacagt tttatttttt
    10681 tcatcggcta gcttctggta tttttcagtg atttctttga tcttttcttt cgaatatcct
    10741 tcaggttcat ccattggttc taccttcaga ttgtacaaaa acccaccgat aaaaccagtg
    10801 ttatagtagt tcattttctg gctgtaaggt atccatagtg ccgttttatt atatgctttt
    10861 cgcaacaaat tatcgggatt gttgaaatta ctgatatacc ctagcagtcc aattgccaca
    10921 agcagtgtcg ttaatcggat caattggaat cgccttcctt ttttcaggct tcgaaacatg
    10981 taccagcaaa acaatcctat cacggttcct gcggcagcta agatcactgt aaacagcata
    11041 gtgccggtca tatctttcag caatccgatt tcagtgatca tcttcaaatc gtctggataa
    11101 atcggttctt gccgataact cattttcata taatttgcaa aacctaatac accgatggct
    11161 acgacatacg taagggaacc taaagggaca gatcctgcta gtgcaatcag aaaaataaga
    11221 ataatcagca agacgagaca ggctaagaag aatttttccg tatgccaaga aaaagcaaat
    11281 ttcaacgcaa ggtctacgga tagatcattt tgacaccact gcaaaaataa attactaaaa
    11341 acaacaagaa aaaagatact gatgattgct gctgttcctt ttaaaatctt tttctgtttg
    11401 gtcgaaagtc tgcttatata tcttttcaaa tgcttgctcc ttcatttcta tcaataaaag
    11461 aatgttgctt ctcttatttt agttgtacga aattcgaaag taaagaacgg aaagaagaat
    11521 taatgaaatc caaatattct ctttctttat ggcctttctg gtttctttaa taagcggacc
    11581 tttcgtactc cagaaatttt ttctagctgt tgtttcactt gattgagctg ttcaaaatca
    11641 gtactgtcta tgtcaatcaa tatgtagaca tgctcattct tatggttacg cgatatatct
    11701 gcgatcgcta gctcgttctc attcagcaga gaaaatatct ctgctaatat attggttttc
    11761 ttttgataaa atatagtgaa tcgaaacggc gcttggaaaa acattcgtgc ggatggataa
    11821 ttgaccgctt ctctaaccgt accgaacaaa agataatctt ttaaggcttc aactgccaaa
    11881 cgtccgctgt ctgccaaagc ttcctgagtc gatcccccta gatgaggcaa cataagtata
    11941 cggtcatttt ctaagaactc ttcttctgga aaatcagtca agtatttagc cagatgttgt
    12001 ttttccaatg cttgcataac agcatattta tcaacaattt cacttcttcc tacattgata
    12061 agtacagcat cttttttcat tttttctata ttcgcctgat cgatcaatcc ttttgtatct
    12121 tctgtcaacg gcagcatgac tacgatgtaa tccgaggtag aaagaactgt ttctaaatct
    12181 gcttgccgaa cataatccaa ctgggcatcg cggatagaat aacccaagac atccatccct
    12241 aagctatagc aacttagcgc tacttttgtg cctatcgctc caagaccaag aagaccgatg
    12301 gttttgcctt gcaattctcg tcccacataa gcacttcttt tgttttctgc ctgttctaaa
    12361 atattaggac ctgttagtgt ttgtaccata cgacttgctt cgattatcgg acgggaagat
    12421 aagagcaaac agcacaaaac aagctctttt actgcattag catttacacc tggtgtattc
    12481 atgacgatcg tcccgttttc agaagctttt tcaacgttga tcgtgttcac cccaacacca
    12541 gctcttgaaa ttgctagcaa atctggtgta agccattcat ctttcacttt cgtactacga
    12601 ataactattc cttcggctgg caaatcatct attattttga aatattcccc ttgtaacttt
    12661 tctatctctt gttctggaat agcatcaatc aaatgaacgt gatacataaa accccctcct
    12721 tactaacaag tatctcgaaa tttctgcaat tagacaactg tttaccattt gacttcctag
    12781 aagcaaaaag agcagttaga gcagagtagg ctgagtgata aaaataaggc tatgacaagt
    12841 tactgccata agccttattt tcgagagcaa aatattgagg gtgagatatc ttgataaatg
    12901 gtgttccgtt agctgacatt gataataaga ctaaatttca aaaaagttga aaagcaaatt
    12961 tcgtcaatct cttcttattt cttaaaacta agtggctttt tcacaacctc tttttatcta
    13021 ataaccactg tttccaactc aactaatttt gcccacgttt caatttgatc tgttgttaga
    13081 tttagcgaga caacggtatg atgaccgcca cctgcctgaa tccatgaatg gacaccttca
    13141 tggaaattcg gtttagtttt ccataacaca cgtgcaacag gtaaattagg tgcttcagtc
    13201 gttggttcaa aggcttctac ttcattgatc agcagtttat aatgcgtacc gaaatctgcc
    13261 atcgatacga ctaccccttc tcctgctttc ccatcaaata caagacgcgc cggatcttct
    13321 ctatctccca ttgataaagg agaaacaacg attcttggtt tcgtcgcagc aagcgtcgga
    13381 tctacttcca tcatatggga ttccaagatt gcttcttggc cactagctag ctcgtaagta
    13441 taatcttcca tgaatcctgt atctttccct cgagccatga ttttcagcaa ccgatcaatc
    13501 gctgcagttt tccagtcgcc ttccccagca aaaccgtatc cttctgccat caaacgttgt
    13561 acagctagtc caggtagttg tttcattcca tacaggtctt caaaattagt cgtaaaagcg
    13621 ttatagcctc cttcttctaa gaaacggcga ataccgattt cctgctgagc ttgtactttt
    13681 acatgttcct cccatgtctt ttcttcataa tcaccatagt cgaaggtata caattccttg
    13741 tactcttcaa ataattgatc gacttcttcg tctgatacgc gatcgattac ttgtacaaga
    13801 tctccgatac caaaataatc aacagtccaa ccaaattgaa tctgtgcttc cactttgtct
    13861 ccttctgtga cacccacgtt tctcatgttg tcaccaaaac gagccacttt tattccaaaa
    13921 ctttcattat aggctactgc aacatccatc caatcagcaa tctctttttg gatctcaggt
    13981 cgtttccagt agccaacgac gattttattt tgtttattta atcgagcatt gatgaaccca
    14041 tattcgcggt caccatgagc ggattgattt aaattcatga aatccatatc gatcgttttc
    14101 caaggaatgc tttcgttata ttgcgtagct aaatgaagca atggtttttg tagaagcttt
    14161 gtcccgcgga tccacatttt tgctggtgag aaagtatgca tccaagtgat cacacctgct
    14221 acttcttctc gataattgac ctctttcatg atttttgtga tcgtatcagc agtaactgct
    14281 aattcttgaa ggcggattgg ataaggcaat tggccatttt cattcagttc atcgaccatt
    14341 tcttgtgcat gtttctttac ttctcgcagt gcttcttccc catataaatg ctgtgatcct
    14401 acaacaaacc aaaattcttt ttctccaata ttcaacataa ttagccactc cttttatttg
    14461 ttttgtccgt aatacgcatt ttttccgtgt tttcgtaaat aatgtttatc taaaatccgt
    14521 tgaggcagct cttctgaaaa tgaattgagc tgtctggtaa ataaattcat cttgcatact
    14581 tcatctagta cgactgcgtt cattaccgca ctttgcgcat ctttccccca agtaaatgga
    14641 ccgtgtccgt gtaaaagtac ccctggaatt gctaagggat caattttgcg ttctttgaat
    14701 gtttctatga taacttttcc tgtttcatat tcgtacccgc tgtcgatttc ctgttgtgtc
    14761 aaaaatcgag cacaaggtac cgatccataa aaagtatctg catgcgtcgt ccccatcgca
    14821 ggaacatcca atcctgcttg tgcccagatc gtcgcccatg tcgagtgagt atggacgatt
    14881 cctccgattt gaggaaatga acgatataaa acagcatgag taggcgtatc cgaagaggga
    14941 ttgagtttac cttcaatgat atgattgtcc aagtccacaa cgaccatgtc gtctgctgtc
    15001 atctcttcat aactgactcc gctaggtttg ataacgaaat atcgtgtttc aggatcaaaa
    15061 gcgctcacat ttccccatgt atatttgacg agtccgtgtt taggcaagtc aagatttgct
    15121 tggtatactt cttctttcag ttgttccagc attttctatc actccttttt cttgcaattg
    15181 atgaatcgct gttttttcaa tgtcgatccc ctcacggtaa cgtctcataa atatctgata
    15241 gcctgctatt tcttctgctg aaggtgtgat ggtgattcct tggtcttgtc caaacacgtc
    15301 ttctgccaag aagttttcta aagacttccc atgtcgatcc atcatatagg ctgctaataa
    15361 cgcgattccc cacgctccac cttctccggc tgtgtccatc actgtaactg gtgcctccat
    15421 agcagatgct agaatccgct gtccaacttc tggtgtttta aaaatccccc catgccccac
    15481 taaacgatca atttggacat tctcagattt caatatatcc attccgattc tcattgcacc
    15541 aaaagcactg gctaaatgga tccgcatgaa attcgctaaa tcaaaacggc tacctggtgt
    15601 acgtacaaat aatggccttc cttcatttac tcctgtaatg ttttcaccgg aaaaataacc
    15661 ataagaaagt aatcccccgc aatccgtatc gccttctagc gctttgttga ataataccgt
    15721 gaataattta tctctgctta tctccatacc caaagaattg gtaaattctt caaaaatact
    15781 gacccaagca ttgatatccg aagagcagtt attcgtatga accatgccta ctagttctcc
    15841 tgaaggtgta gtgaccatgt cgatttccgg atggacattt tctagttctt tttctaatac
    15901 gatcatagcg aaggcggaag taccagccga aatgttccct gtccgtttgg ctacactgtt
    15961 cgtagcgacc ataccggttc ctgcatcccc ttctggcgga caaaccggga ttcctggtct
    16021 caaatttccc gtagggtcga tcagataagc ccctgattcg cttaacttgc cagcttcttc
    16081 tcctgctact agaactttag gcaaaatgtg atccaagtca atcgctacgt gcgctttttc
    16141 gattaactga ccaaaaatat ttttcatttc ttgatggtag gttttcgttg aaggatcgat
    16201 aggaaacatg cctgatgcat ctcctacgcc tagtactttc tcaccagtaa gctgccaatg
    16261 gacataacct gcaagcgttg tcaaaaaatt aatttctttc acatgttctt ctttattcaa
    16321 aatagattga tacaaatgag cgatactcca tctctgcgga atattgtatt gaaatttttc
    16381 cgttaatatt cgttctgctt cttcagtgat tgtatttcgc caagtacgaa atggtactag
    16441 taactgttcc ttgctatcaa atgctagata accgtgcatc attgcactga acccaatcga
    16501 cccgattgtc gtaagattct cgccatagat ttcgaatact tctgctgcca tttttcggta
    16561 actgacgcgc agtcctttcc aaatatcttc taatgaatag gtccagatac cattttctag
    16621 tttgttttcc cactcataac taccagcggc aataggagta aaagatgaat caatcaatac
    16681 agctttgatc ctagttgatc caaactcaat ccctaaagat gtacgaccat ctatgacatc
    16741 taacatactt tgcgcattta cttccttcat aatgatttaa ctccctcctt ctcgtcaaac
    16801 ctgatcaaac cgtcttttta gtagcgctta caatgattat tgtatacttt tgtacgtaca
    16861 tgtcaagcgg ttatttacaa gaatacaata aaaaacactt gttttgtacg tacataagtt
    16921 ataaaatgaa attgcttaca aaatctgatc aaccgccttc tcgaaattct agaaggagag
    16981 aatacttatg aaaaaagaaa aaaaagtttc ctcatcgttt atttattttt ttggcgcatt
    17041 tggcggcatt ctatttggat atgatattgg tgttatgacc ggcgccttac cttttttaca
    17101 gcatgattgg ggtttggcag gtaaagcttc tttgatagga tggatcacgt cttcggtcat
    17161 gttaggtgcg atccttggcg gctctttatc tggtcttctt tctgataagc ttggacgacg
    17221 aaaaatgatt cttctttctg cacttatttt tatggctggt tctgtccttt ctgcatctgc
    17281 ccctcacaat ggttcttatt ttctgattgc agcaaggatt cttctaggtt tagctgttgg
    17341 cgctgcatct gccctagttc ctgcttatat gtctgaaatg gctcctgcta gactaagggg
    17401 aagattgtca gggatcaatc aagtgatgat tgcttctggc atgctccttt cctatgttgc
    17461 agattatttg ctaaaagtgt tgcctgaaac gatggcttgg cgcgtgatgt tagggctcgc
    17521 tgctgtcccc gcgctgattt tatttttcgg tgttttagct ctaccagagt caccgcgttt
    17581 tcttatgcaa tctggccgct tggaagaggc aaaaagagta ttgaactata ttcggacacc
    17641 aaaagaagct gaacaggaat tcgaacaaat ccagctgaat gtcaaacaag aaaagacaac
    17701 cggaacttct tggcatactt tgtttttaga aaaatatcgt tccttagtca ttgccggtat
    17761 tggtgtagca gtattccagc aatttcaagg agctaatgct attttttact atattccgct
    17821 gatcgtagaa aaagcgactg ggcatgcagc gagcgatgca ttgatgtggc cgatcatcca
    17881 aggaatcatt ttagttgctg gttctttgct ctttttggtt attgcagaca agttcaatcg
    17941 gcgaacttta ttgaaaatag gcggtagcgt gatgggactt tcattcatcc taccagctgt
    18001 tttaggaact gtactggatg ctcatacaaa ttcgttgttg attctcttgt ttttatgtat
    18061 ttatgtcgca ttttattctt gcacatgggc tccactgaca tgggtaatca taggcgaaat
    18121 attcccacta gctgtccgcg ggcgagcatc tggcctagcc tcttctttta attggattgg
    18181 ttctttccta gttggcttgc tttttcctgt gatgactgct tctatgtctc aagaaatcgt
    18241 tttcggcatt tttgggatca tctgcttttt aggggtttta tttattcaag agatcgtacc
    18301 agaaactcgt gggaaatctt tagaggaaat cgaacaaagt gcatctaaaa aaacttatcc
    18361 taaacgtatt tctatccatc attcttaaag aggttgtgac agaagtggtc agcttcaaga
    18421 aataagaagg aaccgcttag tttccagtca caaggaacaa tttcaaaaaa cagcttctca
    18481 tgtttgatga atattgtgac ttgtgaccag ttggtaggag aacaccgttt agtggagagg
    18541 tggtgaaaga aacgttcagc cttgagcgat gacaaaagct ctcacaaaca ttgcgacgag
    18601 taaccgtagg agcaataaga ggcggtaaaa acccgtctct tatttttcgt tttctttcac
    18661 tgtttcccgt tcaactagta caggttcata gagaatattt ggctgtgcct gtcctttttc
    18721 aatcgttgtg acgatccacc gtgcggcatc ttgtcccaac ttttcttttg gatgcgtcaa
    18781 tgttgtcaat tttttattcc ctaaatggct taaagaagaa tcgtctgttc ctacgatcga
    18841 taggtcatct ggaatatgat agccattttc tgtcaactta tctagcatta aactagctat
    18901 ttgatcgtta tagcaaatga tgccatctaa agcttgttct tctttcaacc gttgtactgt
    18961 tttttcagcg attgtttctc gtgtttccgt ggtgaaagta atgatatctt ccgtcaaaaa
    19021 acgcacatta tactgttcac acgcgctgat aaaacctttc atcctgtatt ttccttgaag
    19081 atcgtcgatt tttgtgatga acaacagacg ctgatgtcct ttttttatca aatattctgc
    19141 tgccataaaa ccgactttca catcatctaa acaaatataa gaggcattta actcttcata
    19201 agcagcattg atcatcacca tcggaatgcc ttgttctctc aaagcaacat aaagtgctaa
    19261 gtttgggtta tactgattac ttttcgtagg ctcaacgatc aaaccatcta ccccataagc
    19321 aatcattttc ttcaaacact ctttctccaa ttcgtgatcg ttattcgtac tggctaaaag
    19381 caatgaatac ccagattctc gtaattcatt ttcgattcct cggataatcg acggaaaaat
    19441 ataatcagag agatatgtgg tgatgacacc gatcgttttt gttctgttca atggtgtttt
    19501 ttctttctta tagctgtcat ctacatatgt gcccgaccct ttttctttac gcagatagcc
    19561 ctcatttacc aacacagcga ttgcttgccg aatcgtatgt ctgctgacgt catatatttc
    19621 ttgcagcttg aattctgagg gaatggttgt atttacttcg tattcctcac gaaggatctt
    19681 ttgttttatc tcttctgcaa tatcttgata ttttgccatt tgccttgccc ctttttattt
    19741 gtacgtacat atatcataac gagtctgcaa ggtttattca agcaaaaatc aaggtttctt
    19801 gtgctcataa ggcatacgga acaggttcac caaattctgg aaatccttgc tcattccacg
    19861 taaagcattg cgcgttcgcg tgccgattag gattgtctaa cggatcccct ttttggttct
    19921 tctctggtcg agcatgatag atcaaaatat caaagcaccc gtcttctgaa accgtaaacg
    19981 agttgtgacc aggaccaaat tgcttatttt tttcagaaga aacgaatact ggctccgata
    20041 atttatgcca tgattctttt aataaaagat tcttgtgctc atcagcccac agcaggccca
    20101 tggcatagtt ttcatctgtt gcactaccag aatacgtaat gaatattttc ccattgcgag
    20161 taaggactgc tggaccttca tttactgaaa aaccccgttt ttcccattca tactcaggta
    20221 tagaaaggag gatctgcttc ccctttaacg tccaaggatt atccatttcg gatagataaa
    20281 gattagaatt cccagtaatt tcaggatctt gttgggccca cacataatat agctttcctt
    20341 gagaagagaa agttgttccg tctaaactga aagtttccct tttcgttttt atttgtccct
    20401 tttctatcca atctgtattc attggatcta tatgctgatt ttcaagtacg aacatgcggt
    20461 gatgatgaga ttcatcacgt atagttttat gattgcttgc agcaaagtaa atataccatt
    20521 ttccattgat ataatggact tcaggtgccc agatcaattc actcatcggt ccttcttcat
    20581 gagcctgcca tacaatcagg cttgcagcat gttctaattc attcagtgac tttgcccttc
    20641 ttaattcaat cacttggtag cctggaaccg atcctgtaaa ataataataa ccatctgtat
    20701 gtttatatac ccaaggatct gcccgttgcc gaacgatagg attattataa agtgtcttca
    20761 tcataacaaa tttcctcaca ttttctttat tctaattaca ttccaagagt atttttctat
    20821 ccctacattt aattcgtcat gtttttctat tgaaaaatag tttatttcag gttttatttt
    20881 ttctggttct tcacgtgtat ttttgtccaa cagatcgcta ctggttaatg tgtagtgctc
    20941 tccttgatct agcaaattga aatcatgttc aatcgttatc ttttgttgtt catttgtgcg
    21001 attgatcaag aaaatagtca gctgattgcc gttatctacg atcacactgt ccacataagg
    21061 aacatttttt atcttttcac aatcataaac agggcaaatg atttgttgtt tcaatacttg
    21121 acctttagca tatttcgata agtgttgcat cacataataa gttggattcc tccatgcttg
    21181 tcctttagca tctgtcagta ttaaaggtat tgtgtttact aacaatgatt gacaactaat
    21241 ctttacgcga tctgcgtgtc gtaaaatagc cagccctaat gaaccaaata ataatgtatc
    21301 ttccatagta aagtaacacc aatcgatggg actaccgact tcaaaatccc tatgtctttt
    21361 ttccggttgg gcatgtacat tccattcatc aaacgctaga tacattgttt tctcacttct
    21421 caaatatcct tttacataat cacaagtagc aacgatttct tcaatttgac gatcgaatcg
    21481 ataagctttt gcaaggtatg tcgctgtatc atcacgctca tttttaggag gtttagtcaa
    21541 gtcttcttca tcatacttat caatatagtt atgcaaagct aaataatcta cattctcata
    21601 agcttcttct aatgcgatac gatcccattc agggtacgat ggtaaacgtg gcgtagagct
    21661 tccaactaag attgtttcaa ttgaatcatc cactaacttc atgacttttg aggcttcatt
    21721 tgctaaccta ccatattcat aagcactctt agcccccact tgccatggac cgtctagctc
    21781 attccctaaa caccataatt taacgccaaa aggctttttt tgcccatttt ttattcttaa
    21841 atcactccag aaggttcctt ttggaaaatt acaatattct actaaatttt ttgcttcatc
    21901 aattcctttt gttcctaaat tcaccgtgaa tatcggtttc gcttgtacat gactgatcca
    21961 tttaaaaaat tcgtggagtc caaaagaatt tggttctata cctcgccatg ctaaatcaat
    22021 tttttttggt cgttgttctt tcctacctac agtatccacc caatcataac cagatgtaaa
    22081 gtttcctcca ggatagcgaa tgacacctaa ttctaattcc tttacataag agaggacatc
    22141 ttgtctaaac ccgtcaccat cagctgtttc atggcccggt tcataaatcc ctgtatagac
    22201 gacacttccc atatgctcaa caaatgagcc aaacagccga gaactgatct ctcctaccgt
    22261 aaaatcctct ctgattttta ctttcacttc ttcgtccaac agctttccct acctttcatt
    22321 tcctatagtc aagataatag atacaagttt ccatacgcca ccagcaaaga taaaaataaa
    22381 aggagggaaa agaaatatga gcagtacaaa tcctaataac aatacgcaac aaagaatatt
    22441 tctcaaaaaa tcaaaaaata ctttccatgc actttgttca aaaatttgta aaaatgtgat
    22501 atctttgttt tcagaaatca catatatcca ggataaaaga aacatggaat aaataagtaa
    22561 aactacatat ccactaattg aaagtacgat tgtctgttct tctaataatt gattaacaag
    22621 aaaataaata atgatcgtta acagactaag gaacaagttc ttaccaaaat gttgctttat
    22681 tccttgaaag aaaaaataaa tgatatttcc tgatccctca cctttccatt gcgccattac
    22741 ataaaaaaca gcacatgtag caggcacgat agtaataaca ggcaaacaca cgattaccca
    22801 gagaatacct aataagatgt aatcagaaac cacaatcaac tgtccataaa atttagtctc
    22861 aactattttc atcttcctca ctctttcatt ccagacatag ctgttccttg aacaaaatat
    22921 ttctgtgcaa aaatataaac cactagtaat ggcagcattg aaataaccgt tccagccatt
    22981 acaggtccat aaaaagaaat gttctgccct gtaaataaag ctaaccctgc tggtaaagtt
    23041 ctcatttttg aactacttgt caatatcaat ggataaagca agtcattcca actattcatc
    23101 agcgtgaaaa tccctaatga taaaagagta ggtttgctca atggcaacat gattttcaaa
    23161 aaaatattaa actcacttaa tccatcaatg cgtgctgcct cttccaaaga atctggtaaa
    23221 gtaataaaaa aggaacgcat catgaagata ccaaatgcag ttgtcatacg aggaatgatc
    23281 aaacctgcat aagtatcaag catgccaagc aaattgactt caataaatag aggaatcatg
    23341 aagacttgga atgggatcat cattgtcagc aaaacgaaat agaacaaaac tgattttcct
    23401 ttgaaacgca tacgagcaaa tgcatacccc gcaagagaat caaagaatac agaagtgatc
    23461 actgtccctc cagcaaaaat cactgtattt ttgatgtaat ctaataaagg gatcgttgtc
    23521 caaaccttaa tatagttatc tagggtatag ctatctgcaa atattctagg cggaaatgag
    23581 ataatgtctt tttcgtgttt aaaagaagaa ataatcagcc acaaaaaagg aaacgcaata
    23641 accaacaata taactagcat actaatcgtt cgaatgattt ttttaccact aactgctttt
    23701 cgactattca cagtaactgt aatcgagctt tctggtgcca tacaggtttc cctcctattt
    23761 catttgtttt tctttccgtg acatgtacac attagtggag atagctatag ttgcaacaat
    23821 gataaacaaa accgaagaca gtgctgatgc atagcccaat tcataaggtg ctgtaaatcc
    23881 tcgactgtaa atatattgaa cagctgtttc tgtcttaaat tgtgggccac cagcggttgc
    23941 aacatatact tggtcaaaca cttgcatcga accaatcaga ttcgttaaaa tacaaaaacc
    24001 taaggaggga acgatctgag gtagcgtaat gttaaaaaac tgctgtcgat ttgttgcacc
    24061 atccatttga gcagattcat acaaagaggg agagattccc tgaatagcag tcaagagaat
    24121 cgtcatagta acaccaaaat tcttccaaac agtcatgatg gctaccgtcg gcatagcaag
    24181 ggttggatcg cgaaaaaatt gagggatcgg caagccgact tgtgaaaaca agtaaggaat
    24241 cgcccctaca ttcggatcaa gcaacataga aaaaataatc gcaattgacg tcaatgaaca
    24301 aataactgga atgtaaaatg tcgaacgaca aaaacgattg aatctggtat ttttagataa
    24361 tgctactgct gctaataaac caacgataat ttgtgttggc gtttcgagta cggtgaaata
    24421 taaagaatga atcaacgaat taactgctcg ttcatcacta aatatacgtg tgaaattttc
    24481 aaaacttaca aaatttcttg atgtaaagaa aatattcaaa tctgtaaaac taatagcaaa
    24541 tgttcctaaa agcggtatga cagtaaataa taataaaact aaaaaagatg gagttaaaaa
    24601 caaatacgca gcacgatcag gacgattcca atatttggtg aatccgttca tatagtccac
    24661 ctcatctttc attttgataa aatatacaat cttggtttaa acgaatctga ataagatatc
    24721 cgaacaatcg ctgtgatttt tgcaataaca cctatctgca aatactaggt cgatcattca
    24781 gatatctgta tcttgccaac agtctatcct gtataaagtt ctgtactatt tttgcagtaa
    24841 ttgattgatg gcatcatctg catcgttcat gagtgtctca ggatcttctc ctgctaataa
    24901 tttttcaatc agtggattga tgatatcgtt attgatcgca gagatttggg taaaacctgg
    24961 taaatatggc tctgcatagt ccatttgttt cgataattca gatacaatag gatcattttt
    25021 tacttcttca tcttttgcta ctgaatccaa gtaggcagga aaaccatttt cgatactcca
    25081 ttttttacca atttcagttg tattccaata tttcaaaaac tcatagacag cttctttttt
    25141 attattatct gtacttgccg gaatggcata tcctacgcca tctaaaatag ctgttttttt
    25201 gtctgaatca ctcccttgtg gtaaggtagt cacattataa tcaatctcgt ttgctttcaa
    25261 tccgttattt aaccatggcc cattgatttc aatggctagt tgaccagcat tcattaaatt
    25321 gtccatttcg gcgccagaaa tcgattctgg accagtcttg tctacatgga tcattttttg
    25381 gatcgctttt aacgttttaa ggttctcagg agacgcaaat tcagacttag tataatcatc
    25441 gttcactaat ttaccgccat ttgctaacat ccagttatat aacggtgcag ttccatcctt
    25501 attgaagaca aaacctgata catttttact actatcagtt aattttggtg ccatttctgt
    25561 caattcttcc caagtagtag gtggttgttc agtatctaat ccagcttgtt taaacaaatc
    25621 attattccaa tacatataca tgccttgtac ctgcatgggg acaaaatact gtttaccttc
    25681 tacttcacct aattcaatag ccgtttcttt aaaatcagat ttatctactc catcatattc
    25741 ccagaaatcg tctaatggtt gtaccgctcc gttttctaca tactgtgcaa aatctggata
    25801 atttaatgca ataaagtcag gtgctttttt agaagaaatc gctgtaggaa gtttttcatt
    25861 caaattggcc caagtcatga tatccatttc aattttgatg tttttatcat ttgttttatt
    25921 aaattcatta acgatttctt gcaatatttc accatcagat gcagtaaacc cattccaaaa
    25981 agtcaaagtc acttctttat cagaatcaga ttctgctgaa cttccgccac cgcaagctcc
    26041 taaaatacct gttgcagcta ccaacaaaga gccaattaac cattttttac ctttcatttt
    26101 ttgttcctcc ttcatctgat aaacgacatc tgaccacatt ccaagatagc ggtttcaata
    26161 aacaattgat tttcccatct tcagctttta catttgttga atgacttggt tttacttgtt
    26221 cacttttagg aagattagtt gcttttgtat caaatccact catttcagaa aaatcaatca
    26281 ctccctctaa tgataattca gacaagtgaa cagttagatt catttcttct tctccacggt
    26341 taactgcaaa tatgacaagt tctttgtcat ttattacggc tatactttct aaatacggca
    26401 cttcttcaaa atcttccgtc gcatagtttg ctgaacgaat ttgtgggctt aacacctctc
    26461 cttgtccata cacagcagct tgcataaatg gataatagat cgtctgtctc caagcttctc
    26521 cgtttgtttc tgtcctgatt ggtgcaataa cgtttaccaa ttgagcaaga caagcaatct
    26581 ttactctatc tgcatgtttc aataaagtga ttaatagaca gcctaatagt aatgcatctt
    26641 caaaattata gttatcttct agtaaaggag gagcaatctg ccaaggcgct gcttgctcat
    26701 cttgttccgc tgaatgatac caaatattcc attcatcaaa ggacaggttg atctgcttct
    26761 tactatgttt cttcgccttg actgtatcgc acatcgctgt cacttcagaa atgaaccgat
    26821 ccatgtctaa ggaacgtgca agataatgag aaagatcatt tttttggttg ccataatatt
    26881 gatgcagaga caaataatct atttgatcgt acgcttgatc aagaactgtt aactcccaat
    26941 caccaaatgt aggcatttgc cgattcgaac ttccgcataa aaccacctca attgaatcat
    27001 ctacgagttt catcgcctta gccgtttcct ctgctaaccg tccatattct tccgctgttt
    27061 tatgtccaat ctgccaagga ccgtccattt cattccctaa acaccatgtc ttaattccaa
    27121 aagggctctc ttgaccattc gaacgcctta aatcactcca ataagttcct ttaggaaaat
    27181 tacaatattc gactaaatta cgagctgcat ctactccacg agtacccaga ttgacagcca
    27241 tattgatttc ggcccccaca tgctgacacc agttataaaa ttcatgaatc ccaacttcat
    27301 tcgtttcaat tgtccgccaa gctaaatcca accttctagg tctatttgct atgggcccaa
    27361 tactgtcttc ccaacgaaat cctgacacaa aatttccacc aggatatcta atcatgggaa
    27421 tgttcaattc ttttaccaag tgaatgacgt cttggcgaaa tccactttta ttagctgatg
    27481 gatgatctgg ctgataaatc ccttcatata ctgctcgtcc catatgttca ataaatgaac
    27541 tgtacaaccg cggatcaact ttgcctacac gcttgacact actaatcaac attgtggctt
    27601 ccataactga cctcctttaa tttatacatg aataatacac ctcgttgaaa gcgttgtcaa
    27661 taatataaat gttaaactta cttatttttt atcgatttta attaaccaaa atattaataa
    27721 tgattaaaac aaataattta atataacttt tattaacaaa tatgttaata tataagaaaa
    27781 accttttaag aggtgatata tatgcaacta caaataaacg aagaatcctt accggtatat
    27841 gaagcattag ctagtaaaac aaggatcaag atcatccagc tactttccaa aaaaaagatg
    27901 aatgtcaaag atttggcaaa agaacttgga gtgagcagtg ctatcacgac gatgcacgtg
    27961 aaaaagctag aggaagcaaa catcatcaaa acagaaaaag taggacaaca aaaaatttcg
    28021 agccttcgtg tcgataaaat cgatattagt tttccagaaa aaatattcaa tgcttttgat
    28081 acgaaagaaa catcgatacc tattggtcac tatacaaatt atgcaattga accaacatgc
    28141 ggtttagcta cgatccatga ttttatcggg aaagtggatg aaccacgcta ttttatggat
    28201 cctcgaagga tggatgcccg cattctctgg tttaccagcg gatttgtcga gtatcaggca
    28261 cctaattttt taaatacaga ggatacattg gagatgttgg aagtttctgt ggaaatttct
    28321 tctgaatttc ctttttcaaa tgacaattgg ccttcagata tcactttcag cctaaatggt
    28381 gtggagctag gaacatggac gagccctgga gattttgctg atatccgcgg taaatatacg
    28441 cctgattggt atccagataa tcttaatcaa tacggcttgc tgaagacgat ccgaattacc
    28501 aaacacttga cgaatatgaa tggagaaccc ttgtcaaaca tcacgatcaa cgacatccct
    28561 aaagagcaag acacttggca tttgcgaatc gaagtgaaag acgatgccaa acatgtgggg
    28621 ggctgtactt tatttggtaa aggattcggt aattacgatc aggatatcaa attaaaagtt
    28681 tactacagct gatatttttc ttttctattt tataaaacaa aaacagccaa tccctatgaa
    28741 gggacggctg tttttgcttt attgttattt ccagaaatca tccaaaacgg aaattggcaa
    28801 atgtcgttta tgttgtgtct tgttccacca tgcttcgatt ttttcttgtg cttgagcggg
    28861 aacttctttt ccttctagat aatcatcaat ttcattatat gtgacaccta gtgcgacttc
    28921 atctgcaatc attggtttcc catcttcaag atctgcagtc ggaatttttg tgtataattt
    28981 ttccggtgca ttcaatgctt gaagcaattg tttcccttgt cgtttgtcca aacggaataa
    29041 tggcaaaatg tcggctccac catcaccaaa tttcgtgaag aatccagtaa tgttttctgc
    29101 agcatgatct gtccctaata cggctcctgc tctttctcca gctacggcat attgcgtaat
    29161 catacgttga cgtgctttga tatttccttt gttaaaatcg ctgattccca cacctgcttc
    29221 gcttaatact tgtacttgtg catcaacagc tggtttgata tttactcgca gactaacatc
    29281 gggctggata aagtcaagag cagcttttgc atcttcttca tctgcttgtt caccataagg
    29341 aagacgaaca gcgataaatt gatagttttc atcttttgtt tcttcacgca tttcttccat
    29401 cgttagttgc gccaaacggc ctgctaaagt agaatcttgg ccgccgctga tacctagtac
    29461 aaaagtttta agaaacggat attttttcaa atatgctttc ataaagtcaa tacttttacg
    29521 tatttcttct tttggatcaa ttgttggttt gacacctaat tcattgataa tttctgtctg
    29581 caggctcatt tattctcctc ctatgcttct gtatgaagtt ctgctacgct ttttttcatt
    29641 cgttccatga tagccatttt atggttccag cattcagtag acagatcgac tgggtatttt
    29701 tgcggattca agtctcgttt atactcttcc catagtgaat ctagattttc ttttgtatat
    29761 tctttgattt cgtttagtgc aggtaattcg taaactcttt ttccttcgat aaagatatct
    29821 tgtaaaactg ggcgagctgt aaagtctcga acagttttat tgataaacgt atgcactgga
    29881 tggaacatga agatagcttc ttcttctcga gggtcttcat cccataatgt cacgtagtct
    29941 ccttcagatt tcccatcaaa atttcgagtg atgcgccaca cttgtttttt ccctggagta
    30001 gttacttttt ctgcattgct tgataacttg atcgtatcca tcatttttcc ttcatcatct
    30061 tcgatcgata ctagcttgaa tactgcacca agggctggct gatcataggc tgtgatcaat
    30121 tttgtcccaa caccccatac atcaatttta gctttttgca tctttaaatt caaaatcgtt
    30181 gcttcatcca agtcattcga agcatagatt tttgcttccg taaatccggc tgcatctaat
    30241 tgttcccgaa cacgcttaga tatataggcc atatctccac tgtcgatgcg tacgccttgg
    30301 aagttgattt tgtcacctaa ttctttcgcc acgcgaatcg cacttggcac acctgatttc
    30361 aacgtatcat aagtgtcgac taagaacacg cagtctttat gggttttagc gtaggccata
    30421 aatgcttcat aatcatttcc ataagattgg actaacgaat gagcatgggt cccgctagct
    30481 ggtatcccaa atatcttccc agcacgtaca ttgcttgttg catctgctcc gccgatatat
    30541 gccgctctag ttccccagac tgctgcatcc agttcttgtg cccgtctagt tccaaattcc
    30601 aatagtggat cttctccgat gacagatttg atacgagctg cttttgtcgc gatcaacgtt
    30661 tggaaattga ccatattcaa cagtgctgtt tctactaatt ggcagtgcgc taaaggccct
    30721 tccacttgga tcaatggttc atttgcaaaa actagttctc cttcacgagc agaacgaact
    30781 gttgctttaa attcaaaatt ttgtaaatac tccaaaaaac cttcaggata gacttctaat
    30841 ccacgcaagt aagcaatatc ggaatcattg aacgtcagat tctctaaata attgactaac
    30901 cgttccaatc ctgcaaaaac cgcgtatccg tggttaaaag gcatttctcg aaagtaacat
    30961 tcaaaaacag catgtaaatc agctcgacct aattcccagt atgtctgcat catattgatt
    31021 tgatatagat ccgtatgcag cgtcaaactg tcgtctgggt aagtattatt catcttgtat
    31081 tactcctctt ggcaagtttt catttcctac ccattataac aaaaaaaatt aataggaaaa
    31141 gaaatcttcc attttttctg aaaaagcaca tgttttacgg atatttcttg ctaaaaatga
    31201 actccatctg ctaaaactat cctttcttat tggtatttaa cctaaacgag aaagaatact
    31261 tattttattc aatgtatccg agacacgaag aaattagcgt tttctatttt tcttacttga
    31321 taaaataagt agattttcct actacaatcg tagccgttaa gataccgatg attgaacaaa
    31381 tataaccaaa tgtctctaca acataatcaa tggcaaaagt ttttgaagca ataatacgtc
    31441 tttccttttt tgcttcttat ccccaaagaa acggatataa cttattatgt attgaatttt
    31501 tttggaaagg cgaattatga caagatatac cttgcgttga aaaaactcgc ggaaaaaata
    31561 tcacacgttt ttaaaaagat acaaagtaag aaagcagctg tcaatagaat ggaaacaaaa
    31621 atggcgagac atcaatcagt ctcgccttgt tttttattgt atacattaga tatggagcag
    31681 gttaccataa agatgattat tagtcgttat taaatagaat ccttcgtatt tttttgttct
    31741 atcactacaa tatctttatt tggacgtttg aattgtattc cggcacgttc tagctcctgt
    31801 ttcactccta catggacttt gacatcttct cgtgtcattt ttaaaacgtg atgaatcgca
    31861 tagtcgattt cttgccttgt gatttttttc ttcgtattga tcatcacgac ttgcttttta
    31921 tcatatgtgt ctaccaatag cagttgtgtt cgatctaacg aaaataattt gtaataatta
    31981 atagccgtat tcccaaaaaa gaattccact actttaggat aaaatgtctt aatatttaaa
    32041 ttactattca caggtgtttc gattagcttc atctcattcc tcttttctat atagtcgatt
    32101 tagatccaat cttaagttat gttaccatct ttcctagcat taaggaatcg gttacaattg
    32161 aacctttaaa gacaaaagga tttacctttt atcacttaat agtataacat aatttcatga
    32221 gaaaaaaata caattttttc ttgaccatct cgttacgtaa aggattatgc ttacatttgt
    32281 caggaggaaa aataatggag tacacaataa agaaaatgtc agagatatca ggagtcagtc
    32341 cgagaacctt gcgcttttat gatgagattg gactgctaaa accagctagg atcaattctt
    32401 caggttatcg gatttatggc aaaaaagaag tcgatcgtct gcaacacatc ttattttatc
    32461 gaacaatggc attcaagttg gatgatatcc aagaggtctt agacaatcca tcttttgacc
    32521 accagaaagc tttaatcaag catcagcaaa tgttgctgga aaaacgggca caaatcgata
    32581 ccctcttgac aactgtccaa cagacacttg atatgtacga aggaggaaga aaaatgagtg
    32641 acaaagaaaa atttgaaggg tttaaacaac aaaagttgaa agaaaatgaa gaatcctatg
    32701 gacaagagat tcgacaaaaa tacggaaaaa aaaaggttac agcatccaat gaaaaatgga
    32761 tgaatatgga taaagaaacg tatgaagaaa tgcaaactgt tgaaaagcag atgttatttg
    32821 atctcactct ttacttaaaa gaaccagaag atgaaagctt agcagaccgt atcttccaag
    32881 ctcataagaa gtggttatct tacagctggc ctgaatatca gccaaaagcg cataaaggac
    32941 taggaatgat gtacgaagcc gatgaacggt tcactgcata ttatgacgag cgaagtggtc
    33001 atggtgcagc aaaagcttta aatgaaatta tccaaaaaaa ggcgtaatct tttacatgat
    33061 ccaacaaaaa agtacacgta aaatgaccta ataacaaaac agcagacacc caagttagat
    33121 ttttatgcta acttttggtg tctgctccat tctttcacaa gttttttcat cctactttaa
    33181 atatattcat cgttgaataa acgtataagg tattttcact ttatcctttt tttctgtaag
    33241 aaacaattga aatgccattt ctccgcaagc ttccagatgg tggtcgatgg ttgggaaacc
    33301 aagaagctcg ctgatcagca aattatcgcg tccaatcacc actggtgtct tatcttgcag
    33361 atagctttgc aagatgcctg ctgcaaccat atccgaattt gtaaagacac catcaactcc
    33421 aagctcttta aaaaagaagc ctgcttgttt tcctttctct gcatctatac aatcccaaaa
    33481 aacaagtgat tcatcaaaag atggaaaaag ttgatgacat agctgcaaag tgatttttga
    33541 gttataactt agcctgccac ttcgccccaa cgtgatgccc aattgcttta taccttgctg
    33601 cttcaaataa gtaagacctt ctgtaattga ttgttctcga tcaataaaga cacagcttgc
    33661 ctgttttccc ttgatttctt cacaaaaaac tagaggtcca tatttttgat atgccaataa
    33721 atcatctagt gtattggcac gagacgtaat gatcaatcca tcgaaagctt tagctgcaaa
    33781 ttcttcaaga taccttcgct ctttttcttt ctgatagccc gtcggtaaaa gtgtcacttt
    33841 gtaatcataa tcaaacgctg cttcaatgat cccactgatc aactgatcaa aatacggatg
    33901 attggtataa ggcagaatca ctccaatgct tttcgttttt ccataactta actgtctggc
    33961 atttgcatta gggatataat cgacttcttt catgaccgca agtatttctt tccttttttc
    34021 ctcatctaca tatggatgtc cattgattac acgtgaaata gtagaaactg aataacctgt
    34081 gagctttgcg atatctcgaa tgtttgccac tgctgcttcg ccacctatac aaaaagattt
    34141 tcttgtaaat agtctaccac agctggaccg cattcaaaaa actgatacat ttttccttca
    34201 tgcgaaaaac tgatccgctg cccgccccaa tatatatctt ttgtcaaatt ttcgatttca
    34261 ttcattggta tttcttttac tatattccta ttcaaccctt gaaaactgta caacgttaat
    34321 acggatccct tgaactcgac aaacgcataa aaataattgt tcgttgtgcc atctgttatc
    34381 tctacataag tattcattgc aagccctcct tttgatactt tgagtatagt gtatgaaacg
    34441 cgtctcatag caaggagttt gttttgccaa agaaaagttt tattcctctt tcattattac
    34501 caaagtctct tattttaaat tgaatacatt ataatgatac tgtaaatata tctttattat
    34561 tagtgaatgg atatttactt ttaatagata aaatattata actattaaaa ataactattt
    34621 tcattataat tacaca
//
LOCUS       DAHUVV010000021        32390 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.021, whole genome shotgun sequence.
ACCESSION   DAHUVV010000021 DAHUVV010000000
VERSION     DAHUVV010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.021"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            130..2739
                     /gene="clpB"
                     /locus_tag="NK767_001024"
     CDS             130..2739
                     /gene="clpB"
                     /locus_tag="NK767_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCH6694033.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2890..3576)
                     /locus_tag="NK767_001025"
     CDS             complement(2890..3576)
                     /locus_tag="NK767_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694034.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3554..4048)
                     /locus_tag="NK767_001026"
     CDS             complement(3554..4048)
                     /locus_tag="NK767_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCH6694035.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4068..5288)
                     /locus_tag="NK767_001027"
     CDS             complement(4068..5288)
                     /locus_tag="NK767_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6694036.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5435..7270
                     /gene="lepA"
                     /locus_tag="NK767_001028"
     CDS             5435..7270
                     /gene="lepA"
                     /locus_tag="NK767_001028"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCH6694037.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7466..8377
                     /locus_tag="NK767_001029"
     CDS             7466..8377
                     /locus_tag="NK767_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6694038.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            9373..9624
                     /locus_tag="NK767_001030"
     CDS             9373..9624
                     /locus_tag="NK767_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694039.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            9707..9979
                     /locus_tag="NK767_001031"
     CDS             9707..9979
                     /locus_tag="NK767_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694040.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            10069..10308
                     /locus_tag="NK767_001032"
     CDS             10069..10308
                     /locus_tag="NK767_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694041.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            10455..14738
                     /locus_tag="NK767_001033"
     CDS             10455..14738
                     /locus_tag="NK767_001033"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HCH6694042.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            14837..15202
                     /locus_tag="NK767_001034"
     CDS             14837..15202
                     /locus_tag="NK767_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694043.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            15204..15656
                     /locus_tag="NK767_001035"
     CDS             15204..15656
                     /locus_tag="NK767_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694044.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            16270..17580
                     /locus_tag="NK767_001036"
     CDS             16270..17580
                     /locus_tag="NK767_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCH6694045.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            17603..17914
                     /locus_tag="NK767_001037"
     CDS             17603..17914
                     /locus_tag="NK767_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694046.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            18275..19531
                     /locus_tag="NK767_001038"
     CDS             18275..19531
                     /locus_tag="NK767_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCH6694047.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            19528..20073
                     /locus_tag="NK767_001039"
     CDS             19528..20073
                     /locus_tag="NK767_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694048.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            20074..20574
                     /locus_tag="NK767_001040"
     CDS             20074..20574
                     /locus_tag="NK767_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694049.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            20655..21211
                     /locus_tag="NK767_001041"
                     /pseudo
     CDS             20655..21211
                     /locus_tag="NK767_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21231..21575
                     /locus_tag="NK767_001042"
     CDS             21231..21575
                     /locus_tag="NK767_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694050.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            21594..22505
                     /locus_tag="NK767_001043"
     CDS             21594..22505
                     /locus_tag="NK767_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCH6694051.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            22584..23576
                     /locus_tag="NK767_001044"
     CDS             22584..23576
                     /locus_tag="NK767_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCH6694052.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            23573..23791
                     /locus_tag="NK767_001045"
     CDS             23573..23791
                     /locus_tag="NK767_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694053.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            23804..24214
                     /locus_tag="NK767_001046"
     CDS             23804..24214
                     /locus_tag="NK767_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694054.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            24266..26794
                     /locus_tag="NK767_001047"
     CDS             24266..26794
                     /locus_tag="NK767_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCH6694055.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            26810..29020
                     /locus_tag="NK767_001048"
     CDS             26810..29020
                     /locus_tag="NK767_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694056.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            29028..30119
                     /locus_tag="NK767_001049"
     CDS             29028..30119
                     /locus_tag="NK767_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCH6694057.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            30140..30682
                     /locus_tag="NK767_001050"
     CDS             30140..30682
                     /locus_tag="NK767_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694058.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            30877..31134
                     /locus_tag="NK767_001051"
     CDS             30877..31134
                     /locus_tag="NK767_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694059.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            31148..31480
                     /locus_tag="NK767_001052"
     CDS             31148..31480
                     /locus_tag="NK767_001052"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694060.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            31525..32073
                     /locus_tag="NK767_001053"
     CDS             31525..32073
                     /locus_tag="NK767_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCH6694061.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            32080..>32390
                     /locus_tag="NK767_001054"
     CDS             32080..>32390
                     /locus_tag="NK767_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694062.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     E"
BASE COUNT        11083 a         5009 c         6879 g         9419 t
ORIGIN      
        1 tataagtcga gaaaaaaagt caaaaaacta tgtgtatatt ttgaccaaaa ttgaccaaag
       61 cgatataata gtttttgtaa agatcaaaga aataagtgat cttaccttta attaggaggg
      121 tatacctgta tgaatattga gaaaatgact acgacgcttc aagaagcgat tgctgaagct
      181 caacagattg cggtcactcg aaaacatcaa gatatcgaca ttgcccatgt atggaaaatc
      241 tttttacaat ctaatcattt tggtcgaaat ttctatacgg atgccggatt ggatgtcgaa
      301 tcttttgaac atgaaattga tcgtctactg gatgagtatc cagttgtttc tggtggaaac
      361 gtccaatatg gacaaaacct cagccagaat ttctttcgat tattgaatga agcagaccaa
      421 atacgtgaaa gcttcggcga cgagtttctt tcaacggaag tcgttatttt agcgttgatg
      481 aaattaaaaa attatccgtt aactgtttat ttgaataaaa acggattatc tgaaaaagaa
      541 ttgcgtaaaa acatcgaaga gatgagagga ggagaaagag tgacctcaca aaaccaagaa
      601 gaacaataca aagcattaga aaaatacggc gttgatttag tacagcaagt caaaagcgga
      661 aaaatggatc caatcatcgg tcgtgatgaa gaaatccgtg atgtcatccg tattctttca
      721 cgaaaaacaa aaaataatcc tgttctgata ggagaacccg gtgtagggaa aacggcaatc
      781 gttgaagggc ttgctcaacg tatcgtacgc aaagatgtgc ctgaaaattt aaaagataaa
      841 acgatttttt cattagatat gggtgcattg atcgctggag caaaattccg tggtgaattt
      901 gaagaaagac taaaagcagt cttgaaagaa gtcaaaaaaa gtgatggacg tatcctcttg
      961 ttcatcgatg aaatccacaa tatcgtaggg gcaggaaaaa cagaaggaag tatggatgcc
     1021 ggaaatctgt tgaaaccaat gcttgctcgc ggtgaattac atttgatcgg tgccacaact
     1081 ttagatgagt accgtcaata tatggaaaaa gacaaagcgt tggaacgtcg ctttcaaaag
     1141 gtattagtca aagaaccaac agtggaagat acgatcagta ttctgcgtgg attaaaagaa
     1201 cgttttgaga tccaccatgg tgttaacatc catgacaacg cattagtagc agcagctact
     1261 ttatctgacc gttatattac tgatcggttc ttaccggaca aagcaatcga tttgatcgat
     1321 gaagccagtg caacgattcg ggtagaaatg aactctatgc caacggaatt agatcaagtt
     1381 actagacgtt tgatgcagtt ggaaatcgaa gaagctgcac tgaaaaaaga atcagatgat
     1441 gcaagtaaaa aacgcttgaa aaatttgcaa gaagaattag ctgaacttcg tgaagaagca
     1501 aatgcgatga agatgcagtg ggaaacagaa aaagaagaag tcaattctgt ttcagcaaaa
     1561 cgtgctgaaa tcgataaagc aaaacacgaa ttagaagatg cagaaaataa ctatgattta
     1621 gaacgcgctg ctgtattgcg tcatggtacg atacctcaat tagaaaaaga attgaaggaa
     1681 ttagaagcta aagcaaaaga cagcgagatt aagatggttc aagagtccgt taccgaaaat
     1741 gagattgctc aagtcgttgg cagattaacg ggtatccccg taacgaaact tgtagaaggt
     1801 gaacgagaaa aactaattaa attgaatgaa acacttcata aacgagtgat tggtcaagat
     1861 gaagcagttg atgcggtcag tgacgcggta attcgttcac gggctggatt gcaagaccca
     1921 aatcgtcctc taggttcttt ccttttccta ggaccaactg gtgtcggaaa aaccgaatta
     1981 gcgaaagcgt tagctgagaa cttgtttgac tcagaagatc atatggtacg gatcgatatg
     2041 agtgaatata tggaaaaaca tgctgtgtct cgtctagtcg gagctcctcc aggctatgta
     2101 ggttacgaag aaggtggtca attaacggaa gctgtcagac gaaatcctta tacaatcgtc
     2161 ttgttggatg aaatcgaaaa agcgcatccc gatgtcttca atatcttgtt acaagtccta
     2221 gatgacggac gcttgactga ttcaaaaggg cgagtagtag atttcaaaaa tacggttctg
     2281 atcatgacca gcaatatcgg ttcacagtta ctgctggaag gcgtgactgc tgatggaacg
     2341 attccagaag cagtggctga acaagtcaac acgctattac gtggaaactt caaaccagaa
     2401 ttcttgaacc gaatcgacga cacgatttta tttacaccat tgagcctaga caacgtgaaa
     2461 ggaatcgttg acaaaatggt ggcgcaattg gcacaacgtc tggaacatca agaaatcctg
     2521 ctgacaatca gtgatgaagc caaaacgtgg attgctgaaa atgcttatga accagcatat
     2581 ggtgcaagac cattgaaacg atttatcact aaggaagtag aaacaccact agctaaagaa
     2641 atcgttgcag gacatgttat gcctaaatca aaagtaacga ttactttact tgatggacaa
     2701 cttcatttca aaacagaaga gctagaagaa atcgtctaat cttccattga gactataaaa
     2761 gaaaaaaggg gtctgacaca actagtgtca gacctccttt tttcaaataa acagtgttcc
     2821 agaagtagtt tcttcgaaaa ataagccgaa atttcttgaa gctgcccgct tctgtcacaa
     2881 cctcttttat taggtatttt tcactttttc taatttataa ctttcaaagt aacgctcatc
     2941 ttttttactt tgagaccatg caaacaatcc cattaaggca gcacctatcg tatttgtcag
     3001 caagtcgccc atcgtatcca tcaatgctgc tcgtccgaca aacagtgtgc catcagaagc
     3061 agcaaaacgt tgaagattca tgcctaagaa ctggtcgcat aagaattccc agaactccca
     3121 aaacacgccg caaagtccag caaacgcaaa gccgaataag aggaacaacc aaggactggt
     3181 ttttgatatc tcagcatctt tcatcaagta accaatcaat ccatagccaa gagctgtcag
     3241 gaccattgga ctcaccgcat gtaagatttt gtcccaaaaa gagatgatac tgatcaaatg
     3301 catccctgtg cctaaaaaga cagagatgaa caagaaaaac cagtaaaaca aaacgattgc
     3361 tgctggtatt ttaagtttga aaactttcaa aatgacttgt ggcatgaaaa tcaatgcaat
     3421 ccctaataaa gattccacga taaatgtgat tccttgagaa gtcgaatatt tttgtgtgat
     3481 aaattcataa aaattatagc agacaacgaa tacaccaaaa gcaagtaaag ctatttgcac
     3541 ctttttgatt tctttaatca ctgaaagtcc ctccatctaa aaatcttttt acttctgaaa
     3601 gcaactcaat ttctgaagaa accacttttc cgttcaattt gatcaaacct attgtatata
     3661 agttcaggta gtgaaattga ttttcagctg tttcttgtag tgcagcgatt tttttcgggt
     3721 gggttccgcc ttgctgtcga gtatcagtat ataagccgat cactgggatg cctttcgcat
     3781 aagccacacc gatttccgat gctaccccgg catcgatcgt cagcccatct agtaaagcaa
     3841 tcattaaatc actagccaac actttttctg tgtcagcaag agcaatcatt cgactgtctg
     3901 cataagcagt tttgtcattg attgcttcat tttcttgagg aagatagatc gaaagctcag
     3961 aagagagctc tctgatttct ttaactaact gttggttata taacaaatca cttttagcaa
     4021 acattggtgc ggcaaaataa attttcatct aaacatcatc ctttttatca aaaataatcg
     4081 ctaaagtaag cttccatgtt tggaatgata cgctctagtg acttcagtgt ttcttcatct
     4141 gtttctaacc gttcgattct tgccaaataa gaagcacggc gataattcat catcaagcaa
     4201 gtcaatgttt ggatatccag tcgaacggat tttcctatag gttcatttaa gacacggact
     4261 tgtccctgtt catcccatgt taaagcgaag acaccattat tccaaggagc gacaggatct
     4321 tcaatgatga aatgaaaagc atcagctgtt ccaacaaacg gaaaccgttg aaggaattct
     4381 tttacatcaa cgatccgagc cataaaatat ggttcgatct gctctttgat ctgactatct
     4441 tcaatcaaaa atgctaaagg ctcattttta taaatatctc catgtaccca gtagatcatg
     4501 gaaaaatgtg cggaaataaa gttccataag ccatttcgag cttcttgatc aagatagaac
     4561 atttctttta cgtgaaatat ttcttcagct acccagtaga acagaacacc tcttggttca
     4621 tggttgctat cgtagtagac agcggctgtg cgctcatctt cattttcgaa acgccaatac
     4681 tcttcccaat gaaaatcact gcggaataaa gcaccatggt tttgttgggc aaatttgtca
     4741 taaacggaaa atacgtctgg atgatcaact ggtaatcgct caaccatacc acttactggg
     4801 atttgtttag gtagctgtgt atctctgatt ttaaaggaaa gtttatcaga catgatttcc
     4861 catcctttac gccgatagta ggggatactg taaggataca aataagagat ccattgctta
     4921 ttcttgcgca tattatccaa tgcaacgata attagatctt gcatcaatcc atggttagca
     4981 tattctggat aagttccaac tccagtcaca ccacccattt tataacgtgc accgtgaata
     5041 ttgacttcac aaggatagat agcgatttga gagatcagtt tattttcgtg gaaccatccg
     5101 aacactttcg atacttccaa aatcggtttt ttggaacgga taaaagctcg tttattctca
     5161 aacccgcttt cctcgatgtc tgcttcagtc acttggaaca cataggaaag caattcattg
     5221 aattgatcga tatgttcttg agtgaccggt tttaattcaa gattttctct aaaatctttt
     5281 tgatccattt tttcatctcc atttattagc ttgataacag tatagcaaaa ttctacaaaa
     5341 aaaacttcta tagctgacta attaaaaact ctttaaatta ctgcttttcg ttggtataat
     5401 ggaaagtgct gattatatga aaagaggaga cgctatggat ataaagaaaa tgaaagaacg
     5461 ccaagagcag attcgtaatt tttcgatcat cgctcatatc gaccatggga aatctacatt
     5521 ggccgatcgt attttagaaa tgacgcacac tgtgacgtct cgcgaaatgc aagatcagct
     5581 gttagactcg atggatttgg aacgtgaacg tggaatcacg attaaattaa atgctgtaga
     5641 actcaattac acagcgaaaa atggagaaac atatatcttt cacttgattg acaccccagg
     5701 gcacgtcgac ttcacctatg aagtctctcg aagccttgct gcctgtgagg gtgccgtttt
     5761 agtggtcgat gcagcacaag gaatcgaagc acaaacattg gccaatgttt atttagcgtt
     5821 agacaatgat ttggagatcc tgccagtcat caataaaatc gacctacccg cagcagatcc
     5881 cgaacgtgtc cgtcaagaaa tcgaagacgt aatcggaatc gatgctagtg aagctgtatt
     5941 agccagtgcc aaatcaggga ttgggattga agatattctc gagcagatcg tggaatatgt
     6001 tcctgcacca agtggagact tagatgcacc gctaaaagct ttgatctttg actctattta
     6061 tgatagttac cgcggtgttg ttctaaatgt ccggatcacg gacggtatgg tcaaacccgg
     6121 agacaaaatc aaactgatga gtaatggcaa aacatttgat gttacagagg tcggggtctt
     6181 ctcgccaaaa gcagttgcta gagacttttt gatggtcggt gatgtcggtt atatcacagc
     6241 aagtatcaaa actgtccaag atacacgagt aggggataca gtaacgttag cagacaatcc
     6301 tgccgctgaa gctttaccag gttaccgaaa aatgaatccg atggtttatt gtggtcttta
     6361 tccaattgat acttctcgtt acaacgattt gcgggaagct ttagaaaaac tgcaattgaa
     6421 tgatgctgct ttacaattcg aaccagaaac ctctcaagca ttaggttttg ggttccgctg
     6481 tggattcctc ggacttcttc atatggatgt cgtccaagaa cgcttggaac gggaattcaa
     6541 tctagaattg atcacgactg caccatccgt tatttaccat gttaataaaa cggatggtac
     6601 gactgctaca gtagataacc cggcagattt tcctgaacct gtaacgattc aggatgtaga
     6661 agaaccattc gtcaaagcac agatcatggt cccaaatgat tttgtcggtg cagtaatgga
     6721 actttctcaa cgaaaacggg gagaattcat tacgatggat tatttagatg attaccgagt
     6781 aaatgtcgtc tataatattc cgctttctga aattgtcttt gactttttcg acaaattgaa
     6841 atcaagcacg aaaggctatg cctcattgga ttatgaaatg tctggttatc aaaaaagcaa
     6901 gttagtgaag atggacatct tgctgaatgg agaaaaagtc gatgcattaa gctttatcgt
     6961 tcaccgagac tttgcctatg aacgtggaaa agcaatcgtt gaaaaactga aaaaactaat
     7021 cccacgccaa caatttgagg tgccaatcca agcagctatt ggacaaaaaa tcgttgcccg
     7081 ttcagacatc aaagccttgc gtaaaaacgt cttggctaaa tgttatggtg gagatgtttc
     7141 tcgtaaacgt aaactcttag agaaacaaaa agaagggaag aagcggatga aacaaatcgg
     7201 ctccgtcgaa gttccgcaag aagccttcat ggccgttctg aaaatggatg agcaagatag
     7261 tgtgaaataa cttataatca atcgaaaaac ctattaaata aacctttttg agtagatgca
     7321 acactgagga ttatctgtat aaaagatttt aaactgtcat ttggttatca cggcgatact
     7381 aaaattaaca aaattggaat atttttcttg aagattttta gctgtcagta tatgatgaaa
     7441 gcatctcata ggaggtgctt ttttcatggt tgataattca agtggaaggg ttcttaaatc
     7501 tctaagaaaa gagaaaaaac tatctcaaaa aaagttagct gatttagcag gtatttcaca
     7561 aagtacttta gttaaatatg aaaaagggtc aagaaaaatc cctaaagacg tagataatac
     7621 tctaagcaag attctgaata tagaaactct aataaaagat gaggaagata agattgagat
     7681 tctcatcggt aaactaattg catacagaga tatgaataag ttacttaaca aagagttagc
     7741 tgataatata ggaattagtg aagtattact cagttatgtg ttaaatagaa agagaaatcc
     7801 gtcaaaagaa atgcaaaaaa aaatcgatat ttttctatta agtaatgaaa aagaaatttt
     7861 gaaggagatt aatcgtgata gtgagatatt tagtttatca aaagacgata aaattgttat
     7921 gggaaaaaga atacgagaag tccgaaagaa ccgggaagag acgcttgaaa aatttggtaa
     7981 aaactttacc atctatacag gcaaaaatgt tataagtaga tgggaaaaag gtattaatat
     8041 tccagatatt gaaaaactaa tgaatattgc ctatcttggt aaggtcactg ttccttacct
     8101 tatgtacgga gaagattata agaatatact tccaaaagat gaaagagtta gcgattttaa
     8161 aaagataaat tcttttagta tgggattaag gatgaggaaa attaggaaag attattatct
     8221 agaaagggag gagtttggaa agcttttttc tccttcaatt tcaaaatggt ccatagatag
     8281 gtatgagaat ggtagagata tacccaatac taatcgaatt atccaatatg cttatatagg
     8341 aaatctttct ttagaatttt tgatttacgg gatataaaaa tagtttacgt cgatacttat
     8401 taagttatcg acgtttttta ttatagaaaa aatttataaa aggaggtttc ttgatatgat
     8461 aagaaacaaa aagggtttgt tgaaagttgc tttactggca gcttttttct aagtaacttt
     8521 ttgacaagct caatggcaac acaagcggaa gaagtaatac aagaactaac tgttatgaat
     8581 aacagctaga taaaatgggc tatgtatgtt atccaacaat ttagtaatca ctagaagcct
     8641 agcgaaagtt cgcttgataa gttatagatg agtattgatt tgtagtgttt ccaaatcaaa
     8701 agggtacaga gccacatgaa atatgttgtc ctctgcagcg tcaggatggg aatccatacc
     8761 atggagattc ggagatctgt gagggaatac ttacgtggtt aatatgtcag ttgtgttgga
     8821 cgtagtaatg gtcatagtgg gtagattgac tgaattctta tatgagaaaa tgaaaccaac
     8881 aagaagatag ggttatttat acagaattgg ggatcttttc tggaaaagta atcggataat
     8941 gcgagattcg tacggtcttt tttaaatttc tctaaaaaaa acggcatcga atgggttggg
     9001 tgattgataa gcaatgaatc atagactgat atagaataaa aaatgattct atttatcttt
     9061 ttacatctat cgttttttca agtagccgca aattatatca gcattcaaca atttttgtat
     9121 acaagttcta gtaaaaggat ctgaagaaaa agttggttgt tgtgaagata aaaaaagaat
     9181 caggttttac tgagtttttc ttcaaaattt gtgttattag gttaggacaa agataagaag
     9241 aagtatgaag atttaaaaaa ggaatgagga aaagatagta atttccgatt attggagttt
     9301 ttttatttat aaagaagaag cgaataagtt gtgattccta gatttcgcgc gtgattaatt
     9361 aatgagcaaa aaatgcggaa agttttagtg tttaatacac gaggacaaag tttcttaggt
     9421 attgaaggca cacgtgtaac ggttgatggg tggtgtgatt gtttcgaggt tggaaaacat
     9481 gttgaactta tggcatttac tgagctaaaa gataagaaca acgtggaatt tttcgagagt
     9541 gatattggtt gggacgatca tgaagaagtt tatggaaaat ttattttgcg gaagggaaat
     9601 tcttatacga atggggaagc ataagtagcg atttgttaga gatcattgat aggattgaaa
     9661 ttgtcgggaa tatttatgag aatctagagt tatttgaggt gatagaatga gtgatggttt
     9721 tgaacaagta tcaataatta aaaatcaagt tcgggagatt cttagaaaaa aaggctattt
     9781 ggtagatagt tattttgaag gagattacga aacatgggta ggtgtctatg ctcgtccaga
     9841 gaataaacct acctatttag atcctacgac gtcagaagat gcctatttac aaaataggta
     9901 tcgagtagat ggttttaagc aagactttgc ggagtggttt gaatgggaaa ttgaaaacgg
     9961 agaagtaaaa gaagaataag agtacttgtt gtatcaaaat aagccagtag aacaaccaaa
    10021 ccagtagcta gtaatgacaa taatactgag gaatcggatc attatggtat gattgatatt
    10081 tctcttccag aagatcttaa gccagatcct gtaacaattg gcaatagtgt catgattttt
    10141 gattcattag atgaagctat gtcatatggt tggaaagaaa ttaaggatga aggtagtaat
    10201 tggtatggca tgagatttgg tgatggtgaa gttgccaaaa aagctggtat tccaattggg
    10261 aaatggacag ttgatttcca cgaagcaggt tctttttttg ttctgtaatt atagtgggag
    10321 ggtaaaatca ttgatgaaat gtgttcagat aagagcgcca gataaaaata gaagagacag
    10381 ttcacgtcgt tatcactttt ggtaacggcg ttttgtttta ttatataaaa ttctcaggaa
    10441 aaggaggaat tcttgtgtat gcaaaaacga aaatgaattg gttgaaagca ctcgcattgg
    10501 tggctttttc tcaattctgt ttcttggtgg gaactgctca agcacatgca gaagaaacga
    10561 cggctataca acctacgatg gaagccattg cccaagtcgc ttctgaagtg acagaagtcc
    10621 aaccagcaga agccggggtc ccagctggtg aagccactgt tgaacaggcc cctgtgacag
    10681 aagcgccaac ggtcaattcc aaaacgactc tcgatcagac gattggggaa acacaacacc
    10741 cagggaatag tcctggtact tcacaaccag ttgaagaaac tgcctcaatt acaacccctg
    10801 cttcagaaac gtccactcta gaaggagaaa aagctgccga agcaacgcct tctgtgcagc
    10861 cagagtcttc tgagaaacaa gcggaagaaa agaaacgaat agaagaaaca gtggaacaac
    10921 cagcggctac tgctgacaaa gaaggaaaaa atgaggaaaa cgttgaaaaa acaatctctt
    10981 caaccgcagc tagtcaaaat caaccaacga aaccaaacaa agaatctacg gctagtattg
    11041 aaaatcaaaa gaaaaaagag cagaacattc caaaaacagg tacacctaca ctgttagtaa
    11101 cagtaccaac caatcgttcg gttcgtgttc gccgtgcaac caatgcagct gttttaggtg
    11161 gtcggaaaat tgattggggc gaaaatattg gtgttacttc tagtccgaac aatctaaact
    11221 tcactttaaa ttatatggtt cgttttgaga ttaatggaca agtggcgttt tgtattgagc
    11281 ctggtgtctc agccgcagaa ggtcaagttt atacagctac tgctttgaaa aactatttga
    11341 aaaattcaaa tgtacggaaa aaaacaagct tgatctctta tttaggatat gtcacaaata
    11401 aagacaaatc cgatgaacaa tacattgcgg cacaatttat gatttgggaa gctttgggca
    11461 caaaaatccc aatcaaaaat atccgtgttg attatgatcg tcgaaaagca gaaattcaac
    11521 gtttgattaa taactttggt gaaaaagcaa cttttaataa tcaaacacat acggtaaaaa
    11581 ttggcgaaac gttgaaagta aagaataaaa atgatttttt gaaatatgtg aaagacattc
    11641 aaaaaccaaa aggtgtcaat gtatctattc acggcaatga agtatggatt acagtcacaa
    11701 aagaggcacc ggataaagca aaaattcaat tgaataaaat taaagttcaa ggtgcgccat
    11761 tagcctatcg tcgtccagga gctcaaacaa ttggtgtgtt acatccaaca gaacctaatg
    11821 cgtctgttat taatttacaa attctaaaac aaggaaacat tcgtgtgttt aaagaagatg
    11881 cggaaactgg tgtgaaagca caaggtgcag cttctttaga aaatgcggtc tatggtttgt
    11941 atcaagcaga tgggaaaaag attaaagaaa ttaccttgaa aaaaattaac ggcaaagtac
    12001 aggcagaaat taaagatctt gatcctggca aatatgtttt aaaagaaatc aaagcgccaa
    12061 aaggttatgt gctttctgat gaagcaattg aaattgttgt tgaacctgga aaaactgtca
    12121 atgcgaccgc aaaagatcaa gtgatcaaag gaaccattga tttagtgaaa gttgctaata
    12181 aagatttagt cgattcaacg aatccagata acaaaccaaa attagctggc attgaaatca
    12241 ctttgacgtc aaaaacaact ggcaaagtgg tgaaaaccgt tgtgacagac aatgatggtt
    12301 atgctacttt tggtaaaaat accgttgtct ttgatacgta cattctttct gaaaccaaag
    12361 gaaaagaagg gtataaacta tttgaaccac ttgaagtcac aatttctgaa aatgggcaaa
    12421 ctttccatta tgttttagaa gataaagtaa ttgaacaacg tcttaaagtg gtgaaagtgg
    12481 atcaagaaac aggaaaagtg attccacttg ctaatacaga attcaagatt tttgatactt
    12541 tagccaatcg ttatgtcaca atggagattc caaatgatac agaaacaacg gatatcttta
    12601 aaaccaatga aaaaggcttt ttcctaacta acggtacact tacttatgga aaagatcgct
    12661 atcgtttgga agaaatcaaa gcccccgtaa attacgtttt gaatcaaaac ccattagtgt
    12721 tctcgattac caatgataca gaaccgatca aagtgatcca ttttgcgaac cgtcaagcac
    12781 ggaaaagtgt aaaattattc aagtatgaag aatggaacaa gaagaaaacg ccgctcgcag
    12841 gtgtgaactt taccttgtat aacggtaaaa atcaagcaat tggtgaatat accactgatc
    12901 aaaacgggga aattttagtg aaaggtctag ttgccgatga ctattacttc ttggaaaatg
    12961 ccccattaga aagtttccaa ccaaatacag ataaacaagc attcacggtt gcattcaaac
    13021 aaaatgcgcc agatgatggg gaaacgttag tggtttctgt acgtaattac ttgattccac
    13081 caaaactaga aacacaagcg tcaaataaag ccgatggtga aaaagtggtt gatccattag
    13141 aaacgatcgt cattaaagac agtgtttctt ataccaactt gtttgtcgga aaagaataca
    13201 ccatttcagg agtattgatg aacaaggcaa cgaatgcgcc aattttacaa gatggcaaag
    13261 aaatcacaag taccttggcc tttattgcag acaaagtcaa tggcacaaaa gaccttgaat
    13321 ttgtggttaa tgctagtgtt ctgaaaggtc aaacgatcgt tgtttttgaa aaacttttcc
    13381 gtgatggcat tgaagttgca gcacatgcgg atatcaacga tgaagcccaa agtgtgaaag
    13441 tgaataatcc aaccgttcat accacggctg tctttgaaga cggattgaca gtcgcagacc
    13501 caacaggatt gtttacctta aacgataccg ttgaaattac cgagattctt ccaggaaaag
    13561 catacttggt caaagggatc ttaatggata aagcaaccgg tgaacccttg cttgtgcatg
    13621 gaaatacggt agaaacgagt gttggcttta ttgccaaagc agaaaatgaa acggtgatcg
    13681 taccatttac ctttgatttg acaggtataa atggtcgtga aatcgtcgtg tttgaaagtc
    13741 tttttagaaa tgatgaagaa ctagcaaacc ataaagacat caacgacaaa gggcaaacgg
    13801 tacgagtaac taacccaagt gtacgtacaa cggctacaca taaacccact ggcttgaaag
    13861 aggttaatcc attaacgaaa gtcaccatta cagacgctgt gaaataccac gatttggtcg
    13921 ttggaaaaac atacacagtc aacggtgttc taatggacaa agtaacggaa aaaccagtgc
    13981 tagtagatgg aaaagaagtc acaagtacct taacgtttgt ggcagaaaca acggatggta
    14041 cagtcaatct agactttatt ctagacgcac gtgcgttgcg aggcaaagaa gtcgtggtct
    14101 ttgaagatct gtttagagaa ggcgtattat tggcttctca cgcagatatt accgacaatg
    14161 accaaaccat caaaatcagt aatccaacca ttacgacgaa agcgacgaat aaaaatggcg
    14221 gaaaagagat cttggccttg ccaaatcaaa ctgtgcttga atgggtcaaa gtagacggtc
    14281 ttgcgattgg tcaaatctat cgcttaattg ttcaaggcta cttaacacca gacggcacac
    14341 catttgaagg cactcatgcg gaaaaagtct ttgcggccga taaagaaatg atggaattct
    14401 tatttgaatt cttagtggac ggtcgcaatc ttgcaggaaa aggcttgagt tttgcggaat
    14461 ggctccaagc gaaaacggaa gatgagaaag aaacgtttga agaagtggcg aaacacaatg
    14521 tagatctaac aaacaaagat caaacggttc aaattgtgga agcgccaaaa ccaccgcaac
    14581 cagtgaaacc agaacaacgg gttaaaccag ctactgttgt accaatgttg ccaaaaacag
    14641 gtgaacaaac aagtagtata cttatggttc ttggattagt agtattagtc attgctttag
    14701 gtataacact ttataaagta cgtaaagcca aagattagtc atgaggaaca agcggttagg
    14761 atttttaacc gcttgtttga ttgtaatgta gaaatattta gaatttattt tggtttttaa
    14821 gaggaggaaa aactttatgg cagaaaaagc attgcgtatg aagaaagagt ttttaggtca
    14881 agtattttta ttgggttcac gggaacctaa tccaccaaag gaaattaatg gtgtgtctgg
    14941 gcgatatgtt caattacaag cagagtgttc gcgagaagaa cagcctgctt tttttgttta
    15001 tctcacagct gaagaggata aaagattacg attaccagaa aaagaggtgc gtatttcatt
    15061 tgaagaagct tattttgttt ttcgtaaaga acgagtgaat agtcgtgaaa caaatgatat
    15121 gggacaaaca gagcgtcgaa caactggcta tcaagatgtt ggtgtctttt taactggtgt
    15181 aaaaattggg gaggttttgt aagatgggat taaaatttcc aaataattat gacattacag
    15241 aagcattaga tgaaacttgt ttaggagccg tacgctttgg cgcattagaa gagtctgatc
    15301 gtgtatttga taatgaaaca ggcgattata cgggagaaat tcgatcacag cgagcacaat
    15361 tagtttttgg aaatccagat gaaccaaaga ttgcacgacg agttaaattt ccagcagatg
    15421 tagatttgac tggttttaaa cgacgtgatc ctgtgaaatt aattgatcca gaatatcatg
    15481 tggtatatat tccagcacaa gatggcaatc gtgaatattc agatttagtg atttatgcga
    15541 aaggattaga gaaagtagaa gctccaacaa ataatcctaa tatgaaacaa tcacaagaaa
    15601 aaggtttaga tcaaaaacaa ccaagtgttg attctaagga aaaagagaaa aaataaggtt
    15661 tttttgaaag aataggaagg agtcttccta ttctttttat ctaagataga aggaagggga
    15721 gattttgaat tcagataaaa ccaatcaaat actttgagag attgtgcttg ttatcaaaat
    15781 atccaacaat tgattgagta ttttggagac aatccaacga tttttgtaca aacaaatgca
    15841 tatatttgtg cggttattgg gcagtgtatg tttttacgtg atcttgttcg tttttcatta
    15901 ccgtaccagt ggaaacaaga tcatcaggaa gtgatttgga ttcgtatagt acgaatatgt
    15961 aatatgtgtg cacaccctta taaaaatcga ttaaatcttt ttcgattatg tttttttact
    16021 aaatgtgata ttcctgtatt agttgctttt tgtcaaaagt ttttagcaca ggaaaagtaa
    16081 aaacagaaag gaggaatgtg acaattgaaa gctcattttt tcaagtatcg tggtcggcga
    16141 attcgctatt tcaatcgtta tttatggttt tgctattatg gtttcttttt gtttccattt
    16201 ttgattctgt taggttggtt tagttggaaa gtattttatc cacgtttaag tacgttttcg
    16261 aattggcaaa tgtatcttat tcctggaata agtattgtgt tgtatttgtt gctggttagt
    16321 gggttaattt tagggttgat gcattggtcg cgactaaaag aaggatattt tgcctctgtt
    16381 tattggcgtc agttacttgt tcgaatgtta attgacaatc ggtttgttta tacaaagaaa
    16441 caagttagtg agaaacaaac aagggaaaaa atgagattac caaaagttta tttttatcaa
    16501 aagaaacaag aattagtggt ttcttttcct ttagatggtg ggcgttttca cgaacgattt
    16561 ttaaaaatgg gtcatttgtt atctgaagta tttttgcgtg atttaattcg tgaagaacga
    16621 gaaaaagccc gaatcaatta tgtattcctt tcagattctg gtcggattcc tattgatcaa
    16681 tgtatagcag aaaatggacg tattcattta atgaaaactt tggattgggt atatgatcaa
    16741 tcaccaaata tgttgattgc tggtgcaatc ggtggtggga aaacctattt attgtattct
    16801 ttaattcaag cttgtttaag tgttgggaca gtagatattt gcgatggaaa agcggcagat
    16861 ttagcagcat taggtgacgt cgatgtattc aaaggacatg tgttttacaa aataaatgaa
    16921 gaaatgatta tttgtttacg aaatgcatta aaagaaatga atcaacggta tgcctatatg
    16981 aaaaccatga aagatcctaa atatgaacca gggaaaaatt atgcttatta tgggttacca
    17041 ccgcatttta tcttttttga tgaatgggca gctttttatg gttcgttgga tatggaagcg
    17101 agaaatagag tcgataaatt gattcagcca ttagtattaa agggtcgtca agcagggatg
    17161 tacattattt tagcaatgca acgaccagat gcagaatatt ttccaagtgg tgttcgggat
    17221 aatttaacat tccgtatttc tgttggtcga ttgtctccca ttggttattt aatgactttt
    17281 ggagaagatt ttaaaaataa gccattttac aacaaagatc aacctggtcg tggatatgct
    17341 tttccaggat ttgatgtacc tgtagagttt tttgcgccat ttgtttacaa agattatcac
    17401 tttatcaaag aatttgagaa atttactgaa atgattgtct atgattttac agtaaaagag
    17461 gatagtcaag atcaaacgat gggagatgat aatcagttgt tacaagaaca actagagcgc
    17521 caaatgtatt tagcaaaaga tccaaaagga acaagttttt ctgatgcgca tattttgtaa
    17581 tcaactaagg aggtgaagtt aaatggcaga gagttgttat tatagagtag ctacagcaat
    17641 acgaatgatt aatccttctc tttcttccag aacgttttat gattggttga atcgtattga
    17701 acaagtaact gattaccgct ttttgcgcaa agaacgtgtt tttacaggaa aagttattaa
    17761 tcaagtattg ctaacgaaaa aagatataga acgattgact cgattgtatc attatcgagt
    17821 agatttagaa gaggatttaa ctttgtcaat ttatcgcgtt ttttctcctg aaaaatatag
    17881 tgaaataaca aaattagatc atttaatttt gtagttatta tctatcggga gggctctaga
    17941 cgtagtctgg tagccgcact ctgcctggcg gtgcgcatta aggctgcgaa gcatgggcct
    18001 ataatgcgca ctcgctagca gcgtgtgcag ctcacaaaga ggtcgtgcgc ttgcgcacgg
    18061 cctctttgtg taaagccccc cgatatataa tatggggggc agaaataaca taacacatag
    18121 aaaaaagttt tataaaccct tttgtatcaa gtgattatct aaggtcagca gttgtactac
    18181 ttttcccaaa aaacgagggg gactagttga tatatcggtg tttctaaagg gttaaaaaaa
    18241 atcgattttg ttattttata aaaggagcga gaaaatgaca ccgattgagc taaaaagcta
    18301 cagaaagaag ttaagtgcaa ttcataatat aagtggagat aaaaagccgc tatcacaagc
    18361 aagatttgcg caattgatgg gctgctcaaa agattacatt agaaagatgg aacaagggaa
    18421 aagaaaaatc ccagaggatt ttgaacaacg aatcaaggaa cgctttcgat tgtatatgaa
    18481 aagcggaata gatcttcgag caatgattga ttatttgcgt ctttcctttt ttgggacaga
    18541 ctacaaaaca gtgattcaac gtgtattacg aatggatctg acagaatttg aaacacgtcc
    18601 aacgggcttg tatctttttg actgtctttc tgttcgtggg aatatctggg tgttctggca
    18661 ttcaaaacga actgtaacag aaaatgtact tgtgcaattt tcaggacaag gttgccggga
    18721 atatgaggaa gtcatgcgtg aacaacaaac agattggcga acatggctat attccatttg
    18781 gaaaaatcaa ggaaagttgg aagctggtta tacacgtgtg caatgtagcc gattcgatat
    18841 tgcgttagat gaactttggg gattggaaac ggaagaacat ttcgatctct ttgaattact
    18901 agaaaaacaa aaagcaggtt tattagaaat ggactttaag agattccaga atattggcgg
    18961 ttgtttttta gatgatggct attttcgaag tgaaggatta tctttgtatt ttggatcgcg
    19021 aaagagtcct ctctttttta atttttatga gaaacgtttt gaaattgcga accgagaaaa
    19081 aatttcagaa ttagaagcat taacgaaata tggtatttat aatcgatatg agttacggtt
    19141 agccaatgag aaagcaacac aagcagtaga agcatttatt ttaggtgatt caccgaaacg
    19201 tttaggagag attggcgttg gtttaattaa tgcaaaactt attgtttggg atagactgga
    19261 aagtgatgca cggattgttg ataaacgatg ggcaaaatta tttcgttcga tggaagaatt
    19321 ggattttaca gtaaagccag aaaaattctc attagatcgt tcagaacgct ggtttttgaa
    19381 acaagtagct ccgacattaa agttgttaca agaagcagat cgtattttag gaagagactt
    19441 tgtacaagaa gggattgata atgccgaact aaatgacgag caacaaaact atctagaatg
    19501 gatgggaacg tcaggaggga aaattgcatg aatgcatata cgacaacaga atggctgcgc
    19561 ctgttggatt taaaggctgc aatcgaagca ttaaatgaaa agatggttga tttgtcttat
    19621 tttcgttttc gagttcctta tattgaacaa gcggtaaaag ctggacgtta tcaggaaaaa
    19681 gagaactggc aagaaattgc acgcttatta gaagttcgta agggatacga acaagaactc
    19741 gaagagttgg aatttagccg aaggaaagga aggctagaat tcattcgctt ttatcgattt
    19801 tcattgccaa tacctgcgat tttagcggtt aaaaaaggct gcgataaaat gaagatatat
    19861 gaaaactgtg tggcagcact tagcagcgaa aaacctctta tggaagaaat ttcattggtg
    19921 actgtcactt gggaaatgag tcgccagcca acagaagaac agacatatct ttctttagaa
    19981 gagattgaga tagaattaga ggaaattggt cgctatgcca cttgttctac ctattgtgga
    20041 tcggtcatta gtattgctgg tgtgattgtc taaatgaaaa aaattatgat ttatgttggt
    20101 gcgtattggt cacgtgatcc aacagtatta gaaaatccag aggcggtaca ctattgcttg
    20161 cgccaattat tctatctgta taaagaacga ttagaatcgt tgattcgtca gttaccatat
    20221 actgacagac gtttagatga acttcttcta aggtatccag caatgtataa acgacgaaaa
    20281 aatagattgt taccagaaga gtatcccatt gaaaaacatg aacttgaagg gcgatttgtg
    20341 gcttatttct atgatgatgt tcgtatgcgc ttagtagaac aacgaatgga aatagaaaat
    20401 gatcgctact attttatcaa ttattgtaag aagcgtaagt atcaagtaac tgatgatttt
    20461 tatgattgta ttttacaaga tggggaagtg attctatcca aaattgctcc aagttttcgt
    20521 gaacttgtgc cgattgattt tttgaaaaaa tgtcatttgc ggattcttcc gtaaaaaaga
    20581 gctataaaag gaaagaaaag tagaagccaa atcggtttct gcttttttat tttttttagg
    20641 aaggagaaga tggtatgtca ctggaatttt taggagagcc aattgaaaag gaagagttcg
    20701 tttcgcatta tatggatttg tttattcagg tgataaaaaa ctttgtgcga ttgatgaatt
    20761 ttatccacgc gaacaagact atctagccgc tgaacgaata aaaatcagtt tattatggga
    20821 aaaattcaca gatgaaaata accgaccgcc agattatcgc tatttatcag attgtttaac
    20881 gaataatgtg ctttctaaag aatacctatt tcacaaacgt gagaaaaatc aaatgtcggt
    20941 tgaacatttt tgtgattgtt atattgcatt attgcgtagt caacagttat taacagtaga
    21001 acgatatggt gcagagtttg gttatgtcat gcaagctgag cgtcatagag ctacaagatg
    21061 ctatgaaaaa gaagatcaag tgattgatgg ctatgaagaa ttatgtattc gaaacaatcg
    21121 ctatttgcaa caacgtttat atgaacaatt gatgatggat ttttcaaatc tctatagagg
    21181 gtatgaatgt tcgatgattc aagaaaggta gaagaaaagg gggaattttt atgtggcgat
    21241 tattattttt ggtggtatgt ggtatggcat tgttctatgt gtatcgtgtc tatctctatc
    21301 cacgaatgga acgatggaca agttggtata gagatccttt aaaacaacga aaaggattga
    21361 agcggtatcg ttatgaatta cttgttgaat tttcggatgg gcgacaagca cagtattatt
    21421 tttttgcaaa ctatccaaaa tacaacacca gtgaactatt gattcaatta ttcaaaaata
    21481 atccatatgt tgggttagaa gaaaacggac gggagttctt ttatcgttca gataatatta
    21541 gtgcggcgtc tatgaacaaa acacgaattg cataaaaaat tggaggaata atcatgaaaa
    21601 aaacaacatt agcagtcgta acaacactag gaatttcatt attttcgttt caattaggga
    21661 gttcattggt ttatgcagat gaaactgtag aaacacctaa gtcagaaatt gtagaaacat
    21721 tgccagggga aggattacca gatgttgaga cagagctaaa tggaggaaca acggaaaaac
    21781 cagcagaacc tgaacaattg cagtcagaga aacctgaaaa agagccagtg acaccagaac
    21841 cagagtttcc tgaatcaact ttaccgactg attcgatcga agaagaaggt gtgcatagtt
    21901 caacaacaac ggattcaagt atagtcacaa ctccggaaca atcaggaaca accagttctt
    21961 ctgaaaagaa tactgattcg tcaagtacgg aacagccaac gatgtcgtct accagtgaag
    22021 aacaaaaaga agaaccgaaa caacctgttg aaaaatcaga agcaattcca aaagaaaata
    22081 aacctattgt tccagcaaaa gaagtcacag tatcggtaac cccaagcggt gaaattacga
    22141 cgaatacttc tcaaggaatg agtgtaccga ttgtcactag taatgtagag gaattgaccc
    22201 atattccaac accaaccacc ccattaaaag tagcaggtga tcaaacaatt gttggtgtaa
    22261 aagatggtat tccattaatc caagatagtg aagggaattt ggtaaaggat ttgtcgattc
    22321 ctgtaaagaa acttccaagt ggcaatattg aaatcaaaac agcagatgga aaaacaaaag
    22381 tcttaccaaa gactggtgaa gaaatacatg ttgcactttc tgttctagga ggagtgttga
    22441 caagtattgc tggatttgtt ggatataaaa gaaagagaaa aggaatagaa aatgagggta
    22501 attgaatgga ctatttttca gaaaaacaag cttatcgttg tcaatcgtac gtactagtaa
    22561 aaacatacta gaaggagtag cttatgaaaa aacgacaact acggcggaaa gaaaaagttt
    22621 atacagagaa agtaccgaag caaaagaaaa tccattcaaa aaaagcagtg aaatggattt
    22681 ggtgtttggt tttatttctt tgtttttcag ggggcttggc attttttcaa tccatgaaaa
    22741 cgaaggttca agtgtcgttt ttgaaagatg aaatccaaca attacaaaag aaagaagtac
    22801 aaagtcggtc agaattaacg tacacacctg aaattgaatc ctttatgaat cgttttgttg
    22861 ctttgtatat gaatacattc gataatcatg aacaagaaca acaacgtcaa gaaacattaa
    22921 aaaaagagtt ttatgcacaa aactttattc aagaaccagg aagtgtttca ggtgttcgaa
    22981 aattaactgc tgcttcgttt attggattaa aagaaattga aaccattcca actgctagtt
    23041 ataaagtaac ttatgatatt cgctcgatga atccagagag taagaaagaa acaagtgtga
    23101 caatcacaca agtactcaat cttccctttt ctatttcaaa caagggctgt cgtattattg
    23161 cttatcctta ttttacggca ctaccaaaga atacagaaaa acaacgaatg attacttatg
    23221 atgacacatt atacgaaacg gttgatgcgt cggaagcgaa agatgttgaa ggatttgtgg
    23281 aagatttctt agataagtat gcgaaaaaca atgcaaaaga tatgtcatac atgatgaaag
    23341 aacctgaagg actcaatcaa acagctaaag ttgatcaagt agagatgaaa gtattgcgtt
    23401 ataaagaaac gaaaaaacct tttattgtaa aagcaacggt cgtctttcga gatggtgata
    23461 cgtcactttt acaaaaagaa caaatgacga ttgtcattac gaaaaaaaca aatcagtatt
    23521 atgttgaaaa attaacacat aattggttgg ataggaaagg agaagaacgg aaatgacact
    23581 gaagggaatt gtagatttta tattaacgca aggtggctat tttttaataa tggtggttgt
    23641 ttatctggga ttgcgaaatt ttatgaaatc aaaagtagga aatgcattaa tatctatcgc
    23701 agcaggtgcc gcagtttatt tcttcttaaa tgatccaaca cgtgtatttg atgcgtttgg
    23761 taaaatcatt gaaaaattat ttatggatta ggaggatagt gaagtggatt gggaacctta
    23821 tgattattcg attgtttatg cggaacctta tcgcgtacaa cagattacaa gcaattggcg
    23881 tcttccattt ccagttgcat tagtcaatgc agcaactttt ttgttggtcg taatctttga
    23941 atatttattt ttacgttcat ttattggttg ggtcatttct ttttttccac cagcaggagt
    24001 tgttttttat ataggactcc cctatttatg tgtgttagca ctggcaaaac taaaacctga
    24061 tggaaaaaaa gtgcattttt atttatggga ttggttttgt tatatgattc gtatgatgaa
    24121 tggtgattgt gtgtattata actgggacaa ggtatcaaac aatgcattgg aagaattact
    24181 ttttgtgaat aagtatgttt atttagagga ttagaaaaag ggacaatcaa ctggttgttc
    24241 cttttttatt gggaggtgaa attcattggt gcttgtaaaa gaaccagtaa aagcattcaa
    24301 gaaaaattgt ttgttaaatg gagaggattg tatctgggcc tactatcatg tttttccacg
    24361 aacaatttca aaaaacacac caaaaaagac acaggaaata aaagaaatga tggctcgttt
    24421 actgttgctt ttagcagatt atgaagatgt ggagttggtg atgtatccac gaacaatgaa
    24481 tttacggacg aagtttgaca cattaggaaa gggattttct cccgatagtc aagcaattgg
    24541 tgcgtattat gcaaaagaaa cagtgacatt gttggaacga gaaggtcaag tctatcgtcc
    24601 ttcatttatt ttaggaatca aagtcaaaga aaaagcaacg gtggagagtt ttcatgatgt
    24661 aataaatcta atctggttag atttagcagg aagtttagca cgcttatttg gattagaagt
    24721 agatgatttg gaaactttgt tttctgaaag tgatccttat gtaaaagaag tgacacaatt
    24781 actttatagc ttttctattc gtcccttagt aaaaaaagag ctgttgtatc tcaatcgtct
    24841 gcaatatatt cgaggaatgg ctcacaaagt agaagaacaa aataattggg aagtactgaa
    24901 agacaatcaa ggaacgtatg tacgtatacc gaaggaacat aaaggccttc ttcaattaga
    24961 agatgagtta gggagtagtt atttagcatt gttgcctatt ggggaaacac ctgaaaatat
    25021 gagaaattgt gatctatttc aatttgctca ggatctttct ttcccagcag aattgcgaat
    25081 gaaaattcac tatccacgct tgaaaggaca gggaggtatt ggtttaaaac tgaattggct
    25141 atcggaacgg ttcaaaacag agcataagga attggttcaa aatgatgaag aacctagtga
    25201 acgcttatta actgttcgag gattagtaaa acatttaaag caacgagtag ataatgggca
    25261 acctgtcgtc gattggatcg catgttttat tgtatacggt caaacggttg aagaagttcg
    25321 tcaaaatagt gcaacggtta ttcaacaact tcatacgatt atccatatcc accgtgcaaa
    25381 aaatgatcaa cgagaattgt tctataaatg tttattaggg caaccattgg gacggcagtt
    25441 acattggaag caccgaacaa ctgtagaagc agttggcgaa tggttgtttt cagcagtatc
    25501 agaattaggt atggaatccg gctggtatat tggtcggcaa gatacattag gggatggtgg
    25561 ttctcgagga gaaaaaacac cattagaaga attaatttat gcgtcaaatc gttttgtctt
    25621 tttgaatctt ctagccattg cggaaggaat caaaggtgcg ttgtttgatg cgcctcatgt
    25681 agcagtaact ggaaaaaccg gtaaagggaa gaccttttta gttggcttgc tctttttgta
    25741 tagttcgttt atgaatgtac aaagtttatt tgtggatccc aaaggagaaa agcgcaaatg
    25801 gtttacgaaa ctcattcaag acccgtatta tcagaaacat taccctttgt ttgtagaaca
    25861 tttgcaaaaa atgagttatg tcacgttaga tgctacaaat ccccgtaatt atggtgtatt
    25921 agatccaatt gtttgtttat cccgagtaga tgcgaaacaa gtagcccagg atatgattga
    25981 tgagttaagt ccgttaggta ccaatcacct tttaaaaggg gcggtattaa agggaatcca
    26041 agaagtttta gttcaaaaag aagccggaca acaagttggc ttgatgcatg tggtcgacta
    26101 tttagaacaa atggaacaaa aagaggttcg agaatatggt gagtttttac gcttgacggt
    26161 agaagattcg attttacgtt taggattttc ttatggagaa aacccgggat tagactttaa
    26221 tgccaaaaca acgattttag agatccaaga tttgaaatta ccaaatgaca atgtccgacc
    26281 agaactatat acagatgcgg atcgaaagtc tttgtgtttg atgattagtt taggtcgttt
    26341 ttgtgaaatg tttggaaaac gagatagtag taagaaaaca gcgatttatt ttacagaagc
    26401 atgggtattt aataattcga acgcaggacg tagtattatt gcagcaatgg cacgtgtggg
    26461 gcgttcccag atgaatcaat tagtgttaga tacacaattt attggtgact taggttcaga
    26521 taaagaaaaa gggaattttg gtgtcgtctt tgcttttgat gaagacagtg agcgagaaaa
    26581 gattttgaat catttacgtt tggaagtaaa tgaagcgaac ttagctgaaa tgagccacat
    26641 gattaaagga cagtgttgga tgttagatcc ctatggacgt gtaggaaaac tcaatgtaca
    26701 cagtttgttt gaagaaatga ccgctgcatt acaaacaacg gaaaaaacaa gtcactctca
    26761 agtagaagaa caggatacgt taatcatgca atagaaatga ggtgaaacaa tggcttatat
    26821 aaagcataag gaaaagaaat ggtgcgtttt gtttttttta acgattggat tcggactgct
    26881 ttttgttggt ggattggtgt atgcggcaga aaagcaggtg gatcaatcga tctatgagat
    26941 tgggaaattt catattgata ccttttcttc ttatttatct gatagtgaat tttcattagg
    27001 caaaatgtta tttggggcaa atggcacaga ttttctaaaa atgatgacga atatgttctt
    27061 tagtttaagt aaattgattt ggcaaggcct tgattttgtc attgagaagc tctatgaagg
    27121 agctgcaatg aacgagttaa ttcaccagtt tttcactttt tcggatacta tttattcaaa
    27181 aatatacgat cctattggtg cggggattgt cgtcttgtat gtgttatatc tatttactct
    27241 tcgtatattt agagggaaac attacgcaaa aatggtgttt attcgatttc tcttcgttgt
    27301 cattttttca atggtttggt ttggctatgg tagtaacgta cctagtaaag gagaagcttt
    27361 taccaaaggg ttaaatgatt tgtccgtgga ggtagaaggc tttatttttt ctgctacaaa
    27421 tagtctagat gaattggcga caaaagatgt caatggtgtt ttagtgccaa cgacacaaaa
    27481 ggaagccatt caaaaagtgc gccaacttta ttataaagcg gctgttgttg atccgtatat
    27541 attgttaaat tatgggacaa caaatttaga agaattgtat caagcagaga ttgaaccaca
    27601 agaatttcta gccaaggaag cgtcaagtga agaattatct gatattgaga caaaagtgac
    27661 cactgctgca ggagaagata gagggaaaaa gaaggcttat cgtgtctata ttcaaccaac
    27721 taaagctgtt tataaagcag tcgttggttc tatggtgcca ttgcttaatc taagtattgg
    27781 attaccactt gtagtgattg gcatgattcg ttttatcttt caaatgttgg tgttgtgttt
    27841 attacttgtg attccttttt cattactcgc ttcttttgtt ccagtatttg attatatgct
    27901 catgaatacc ttaaagtcct ttttgggctt gatgtttcaa aaatcttttt acagtgtgat
    27961 tattttggtg gcatttttga tctataacat agtagacgat ttaattccaa tgaatactgt
    28021 ttttggtttt ttaggaaata tgtttgtcaa agcactccta tcaattttac tcattgtaaa
    28081 acggaaagat ttattaaaga aactagggtt aggagctgca gatcaaacta taaacaacac
    28141 gaaaaacata gcaagtcatt ctttgtctca aatgaaacaa ctgcctggtc aactatcaac
    28201 tgctggacaa aatttggcaa tcaaaggtgc aggattagca gggaatgtgt atcgagcaac
    28261 gaatccactc gcttctgcag cgatacatgg atttcaaaag tttcaacaaa aacgtacacc
    28321 acaagaatcg gtaaaaggga ggcaagtggt taatcccttt gaactaggtg ggcgttcaaa
    28381 tcaagccttg ccttatagtg gtcgtcatgt tcgttatcat ggggtagagc ctgaaatgtc
    28441 ttctaccaaa tcgaaaacag agtggaattc tggtctttca aatggtttga ttccaaattc
    28501 gtccatgaat tcagtaagac caaaacaaca ggtacaatca ttggcacaat tgagtaagca
    28561 acaagggaat aatcaaagaa aaccattgtt aaataaaggt atcgcttttg ttggtcaatc
    28621 gattccgttg ccttctggaa aagtacccgt gccacaatta acagctgctg gttctctttc
    28681 acaacagatt cgccaagtga aaaatcaggc gaaaaatggg agttatccag caatgcagca
    28741 gttattgaac caatactctg ttcctaattc gtcggtagcc actctatcag gaaatcgttc
    28801 tgtgcaacaa ccggggcaac gttcgtctga aagtaaaccg tcgatttccg caccaaaatc
    28861 gttacaacct gcattttctt ctttagataa gccaacagag caatcggaaa aaatagaaaa
    28921 ggatcctaaa acactgccga ttttggacgc aaaaccagga actgatttta ctttaccaaa
    28981 aggacgaaca gtccaaaaac cattatcgaa gaaaggatga ggaagaaatg aagaaagggt
    29041 gtctaggttg tcttcttttc tttattcttc ccttttttct attgccattc tttttttcag
    29101 gagaactttc tgagcatgga ggagaagcta caggtctagc tgtcgctttt aatggggaat
    29161 ataccgaagg attaccgatt ttttcagaga ttaaaggacg tggtccaatc acagatgaac
    29221 atgcgcaagc agcagttggt gcagctgtaa agtatcagtt attaccgagt gtgattcttt
    29281 ctcaattagg ctatgaatca gcttatggac agtcacaatc tggaagaaac gatacgaact
    29341 tttttgggat tacctggttt cctggttgtc cctacccaca aggttctgca cgtggaattg
    29401 gtggttctga aggtggtttc tatatgaagt ttccgaactt aaaagcttgt tatgactatt
    29461 atggctttat ggtggcgagc caaagcaact ttaatcaatg tgtgggcaat aaagatcctg
    29521 gaagttgttt attgattcta ggtcgtggag gctatgcggc agcaggtatt accgaaggga
    29581 gtccgtatta tcgaggagca atgagtatca taacgtccta taacttaacg gaatatgata
    29641 cttttgcgat caagcattgg aatagcctac caacaaaaaa aacttcgctt attgcaggaa
    29701 aaagtaaagg caatgcgaag gttttggatc ggatcattgg acgatccgta aataatgggg
    29761 aatgttatgg tggtacggca tggtatgtat ctcaattagg tggcccacag ttaatgggtt
    29821 ctggacacat gtttgcgatg ttaatagggg aagattataa ctggacaacc tatggttggc
    29881 aagtaatcat gaacccaaaa ccaagtgatt tacaagcagg agatgtggtg aattggcaag
    29941 caggtggtgc attgtctcct ggaatctatg gtcacaccgg aattataaca gatgtttcat
    30001 atggcggaca acatttctct agtgtggaac agaatgccga gcaaggacgg attatggctc
    30061 ggtatcaacg cacgtacaat caaacaaaaa ttcgtagtct ggttcgtaag gtgaaataag
    30121 aaaggcagtg actagaaacg tgaaaaaagg gataatttta gtatttagtt ggttatgttt
    30181 gataggtctt actggttgtc attggttttc ttctgaaaaa caaaaaacac aattgagaac
    30241 gacagaagaa acaatcgaag cagtaaaaga aacaagtgga actcttcaaa gtactgaaag
    30301 taacagtcaa attggttcat tagaagacga gacagcgcaa caaatgcaaa tagatgaaat
    30361 gacaaaaaat ttcttaaaag attttgctag aaaatggagt aatttcacag atgtttataa
    30421 acgcaatcaa gctgtgaggg actatatgac agatcgttgt attaaagaca atagtattga
    30481 tgtagacccg catgtacaaa tgaaaacaac tgggaaactt taccaggtga cacaagatat
    30541 agagaatccg gatcaatatt tgttatttgg agaagaaaca atgaataagg ttactcaatt
    30601 tgtattactt caattgaaag tagatcaaga agctcaaaag attgatgcga tcaagattta
    30661 ttatgtacgg agtgcgtatt aaaataaaaa atgaacactc atatttatta tacaccgaag
    30721 aaatagaaaa aaacagactg tttttttaag aaaaactatg tcattcttta cttgaaaaaa
    30781 gttgttatag gacagaaagg ataaaacaaa aacggaaaaa atatgtctta tgaataacta
    30841 taagaactat tctatttttt aggagatgga tacgagatga aaaaactaat tggctatagt
    30901 gtgatgtgta ttggatttgt tggtgtggga ttgtttgtat cgactgaatc tgcttctgcg
    30961 gctgaatggt ataatcctat ctggtataat ccgaacattg aatgtcaaaa aggctatccg
    31021 tatggaccga caaatctgaa aaatggaaaa tgtagagtaa attggaatgg cgttgtagat
    31081 agtgtaatta ctggttcaat ggactctgct ctacatggct ttacgggtca ctagaaggag
    31141 gaagaaaatg tctgaagaga aaaaagaaat cgttgaatca ttagtagctt taaaagattc
    31201 tctttctaag attgaatatg ttgataggca agaaattcaa aaactaattg atgatactat
    31261 tattgaaatc caagatgcgc gttgtgaagg tataaaaatt tctgtagctt tatctaaagt
    31321 aatagaaaaa atgaaccgtt cattagcgtt taatggattg aagctagata gacaaactag
    31381 tctgatatgg gatcatttaa aagacttgta tgacaagtct aaaagaagtg aacggactgc
    31441 tgtatcaatc ttaaaaggcc tttggggaat gaattcataa acaagatatt taaaaagtaa
    31501 cttatttttt atggaggaga aattatgaaa agaataatta aaatatttgg tgttttaggt
    31561 gttttcttat taggtttaag tacttttgtt agtactggaa caagtgtgta tgccacagaa
    31621 aaagtgaata cagttgattt agaaataaag aataaattag tagatgaatt aactttttat
    31681 tttgatacag ttggtttttt agacgaacat ggtgtatacc atataaagga ttttggtgct
    31741 attcgtgaac gaatcagtca agataacgaa caaggaagag cagcaagagt cttttatgaa
    31801 catattattg ttcctagaat ggagaaagtt gattggaaac gatatgctgg atgtgtagcc
    31861 attaattctc ttcctggagg aggagtggct tgggctatag gtacgcaggg agattttatt
    31921 gaagcattaa aagcttttaa ttttgaaaaa gctgcggatt tattattaaa aattgcagaa
    31981 aaacaattag gaaaagctgc agctaaagag ttagctaagg taaatattgt agcaggaatt
    32041 gcagcagctg ctgctagctg tgcattagag taaaagttta tgaaaagttt agaacaagag
    32101 catatggctt ttaaacaagc aatgtttaaa gaaaatatct atttaaacca taactatatt
    32161 cgtgtttcta aggcatgttc accagtattg aatatgcttg gaggtggaaa tggattatat
    32221 catttactgt ttgtcgatgt ttgttggtta atttttttac cagatgaact agttatagtc
    32281 aatgaaaaaa taacaagtaa aaatgaagtg tttaattatt ctttaacaag aattaattac
    32341 aaagagatta ccaagttttc actagaaaaa gttccttttt ggggagaatt
//
LOCUS       DAHUVV010000022        29508 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.022, whole genome shotgun sequence.
ACCESSION   DAHUVV010000022 DAHUVV010000000
VERSION     DAHUVV010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.022"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..839
                     /locus_tag="NK767_001055"
     CDS             <1..839
                     /locus_tag="NK767_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCH6694063.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
     gene            complement(1263..1433)
                     /locus_tag="NK767_001056"
     CDS             complement(1263..1433)
                     /locus_tag="NK767_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6694064.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1627..1806)
                     /locus_tag="NK767_001057"
     CDS             complement(1627..1806)
                     /locus_tag="NK767_001057"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694065.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2193..3305
                     /locus_tag="NK767_001058"
     CDS             2193..3305
                     /locus_tag="NK767_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCH6694066.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3383..3676)
                     /locus_tag="NK767_001059"
     CDS             complement(3383..3676)
                     /locus_tag="NK767_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCH6694067.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3844..4158
                     /locus_tag="NK767_001060"
     CDS             3844..4158
                     /locus_tag="NK767_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HCH6694068.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4179..6158
                     /locus_tag="NK767_001061"
     CDS             4179..6158
                     /locus_tag="NK767_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCH6694069.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6171..6497
                     /locus_tag="NK767_001062"
     CDS             6171..6497
                     /locus_tag="NK767_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HCH6694070.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6513..7031
                     /locus_tag="NK767_001063"
     CDS             6513..7031
                     /locus_tag="NK767_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694071.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7031..8383
                     /gene="celB"
                     /locus_tag="NK767_001064"
     CDS             7031..8383
                     /gene="celB"
                     /locus_tag="NK767_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCH6694072.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8467..9972)
                     /locus_tag="NK767_001065"
     CDS             complement(8467..9972)
                     /locus_tag="NK767_001065"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCH6694073.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10064..10654)
                     /locus_tag="NK767_001066"
     CDS             complement(10064..10654)
                     /locus_tag="NK767_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCH6694074.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10833..12479
                     /locus_tag="NK767_001067"
     CDS             10833..12479
                     /locus_tag="NK767_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCH6694075.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12528..13247
                     /locus_tag="NK767_001068"
     CDS             12528..13247
                     /locus_tag="NK767_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCH6694076.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13726..14673
                     /locus_tag="NK767_001069"
     CDS             13726..14673
                     /locus_tag="NK767_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6694077.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14685..15605
                     /locus_tag="NK767_001070"
     CDS             14685..15605
                     /locus_tag="NK767_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6694078.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15646..17103
                     /locus_tag="NK767_001071"
     CDS             15646..17103
                     /locus_tag="NK767_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCH6694079.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17448..18062
                     /locus_tag="NK767_001072"
     CDS             17448..18062
                     /locus_tag="NK767_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCH6694080.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18055..19779
                     /locus_tag="NK767_001073"
     CDS             18055..19779
                     /locus_tag="NK767_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCH6694081.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19776..21227
                     /locus_tag="NK767_001074"
     CDS             19776..21227
                     /locus_tag="NK767_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6694082.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21700..23853)
                     /locus_tag="NK767_001075"
     CDS             complement(21700..23853)
                     /locus_tag="NK767_001075"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HCH6694083.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23855..24901)
                     /locus_tag="NK767_001076"
     CDS             complement(23855..24901)
                     /locus_tag="NK767_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6694084.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25051..26340
                     /locus_tag="NK767_001077"
     CDS             25051..26340
                     /locus_tag="NK767_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HCH6694085.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26353..29055
                     /locus_tag="NK767_001078"
     CDS             26353..29055
                     /locus_tag="NK767_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCH6694086.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29206..>29508
                     /locus_tag="NK767_001079"
     CDS             29206..>29508
                     /locus_tag="NK767_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCH6694087.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPI"
BASE COUNT         9609 a         5107 c         5976 g         8816 t
ORIGIN      
        1 catatcagct ggtagaaact caagcgccaa cggggtatga attagatgcg acaccgatcg
       61 agtttgagat cgaaagaagt cagacagcgg tggtagaatt gacgaaagca aaccgactta
      121 caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag cggggaagtc ctgcaaggag
      181 ctgtttttga gttacaagat gccaatggaa ccgttctgca gtcaggtcta accacaggag
      241 cagatggaaa actggcgatt gacggcttag caccaggagt gtatcagctg gtagaaaccc
      301 aagcgccaac ggggtatgaa ttggatgcga caccaatcga gtttgagatc gaaagaagtc
      361 agacagcggt ggtagaattg acgaaagaaa atcgacttac accaggcggt gtcgtgttga
      421 caaagatcga tgaccaaagc ggagaagtcc tgcaaggagc tgtttttgag ttacaagatg
      481 ccaatggaac cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag
      541 atggcttagc accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat
      601 tggatgcgac accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa
      661 caaaagagaa tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca
      721 gtggaaataa gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa
      781 tattgggtgt gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag
      841 ctaaaataaa aaattgaata agcatagttt ttaaaatcaa ctagtaccca tcaattgaat
      901 ggaaactacc gattttagaa ggggtataca aacaaatatt agtatcggag tagagaaggt
      961 tgaacccaac cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag tgaaaaataa
     1021 acctttgtag taatactagt taataaaaaa tatacaaaaa cactgcgaaa atcattcttg
     1081 agtttcgcag tgttttttgc ctaatgactc aagtttattg ggtaatactt tgattttctt
     1141 tctacaaata atcagcgatc agttgtaatt ttttctctag ttttaatggc tctttgtcaa
     1201 taaggagaaa aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact cgtgaataaa
     1261 ggttacgcct ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac caatttcgca
     1321 tttggtagaa catgtttggt gagctgaaag taggtggcgt tcatgtctgt aactaagaat
     1381 tctatttctt taggattagt gcagctaagg aaatagtttg ttaatcgagg aattttttgt
     1441 gtaggtaaat tttctcgatt aatttttctg tctcgccatc cgcggaaata aaactcagtt
     1501 tatcctcaac ggaaacgtga gaacgaaatt cacaaccctc aacactcaag acagaattct
     1561 tttggaagac tttggtaaat agcttctcaa ctctttcaat gtgcgaataa cggttgtcaa
     1621 agatacctaa caacttttcg aaataaaaaa taacgacact ttttcagtca gtaaagaagt
     1681 gattttatgt ctgacatgat ttgaaatcga gtgccttgga cggataaaat aactttgagc
     1741 gttccaatgg cttcgacagt ttttacaggt atagcgctgc ttttttaggc gtataaccat
     1801 tggcatatga ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac
     1861 cattaatttc ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc
     1921 agggaagaga actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac
     1981 ctctgtaatc atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat
     2041 aaagcatatc gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat
     2101 atgttttttt ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata
     2161 tgatgttcca tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga
     2221 aaggacgaca acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac
     2281 aaaaaatacg agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt
     2341 tacgatcatt attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat
     2401 ttgaatcgaa gcataaacca tacgctgtgg ttttattctg tatgttgatg agtattcgtt
     2461 ttgttgactt tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct
     2521 cactattagt agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc
     2581 attttatttt actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg
     2641 cttctttgta cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga
     2701 acaagaattt ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag
     2761 ttctccttta cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt
     2821 ttttgaaagg catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta
     2881 gtcttttgat cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg
     2941 cattttcttc tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag
     3001 tagaccgaat cattggctca ttgattggat gtgttttatt tattggtatc agccaattta
     3061 ttccttttaa ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata
     3121 gatataaaac agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg
     3181 ttccaggagc agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca
     3241 tgtatattgg ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc
     3301 attagatgag atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg
     3361 ctatcattcc atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta
     3421 atttatactt tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt
     3481 cgattgcttc ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg
     3541 gaaagtaata gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc
     3601 tttcgaccat ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat
     3661 tcactgtgat actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg
     3721 ccagaaatta ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata
     3781 ttttgaatag aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga
     3841 tttatgaaaa aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca
     3901 aagacaacgg atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct
     3961 acagaaggcg ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa
     4021 acgacgatgt ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt
     4081 agtatccctt tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg
     4141 atcgaagaga acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc
     4201 tgttgagata tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg
     4261 aattgttatt atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag
     4321 aaaaaaacgg aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac
     4381 aaagactgac atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag
     4441 aaccgcaagg attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt
     4501 tggaagatgc cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca
     4561 gtttgacaaa ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg
     4621 tttctaagca tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta
     4681 tcatggatac tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa
     4741 gccgtttttt cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa
     4801 gagaagctaa gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt
     4861 tggggatcaa acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag
     4921 tttctaagcg agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg
     4981 caggtgtatc gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt
     5041 cgaatcacaa agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga
     5101 aaagattgtc tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac
     5161 tactcgatca tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc
     5221 cattgactga ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt
     5281 tcagcaacat gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg
     5341 ctttgcatct catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga
     5401 ttatctgtgc aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat
     5461 tcggtaaaaa tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt
     5521 taaaaggaat cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc
     5581 cagttttaca tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag
     5641 ttggagaatc tataccctat cattcctcag atagaccgat accagcattt tctctacagc
     5701 aaaaaaagca gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg
     5761 aaccgaaaaa ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag
     5821 aaggatatat aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca
     5881 tattcagtga tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg
     5941 tagccgttgc attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg
     6001 tttttttagt ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa
     6061 tcgttaggtt agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg
     6121 agaatttcag cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa
     6181 aaataaacag tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg
     6241 cacggacact gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag
     6301 aagagaagtt gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac
     6361 ttctgaaaga atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac
     6421 aagatcatct gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt
     6481 atcaaaaaaa taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg
     6541 aattagaaaa aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac
     6601 gttacctgac tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag
     6661 ctaaaagttt aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag
     6721 gagaacaagt aaagctttat cgaaaacacc aaacgaacgt tccatggaca aagtggtatt
     6781 ttttgttcca gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta
     6841 cattcttgta tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg
     6901 ttgtaagcgg tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca
     6961 accaagacca acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg
     7021 agaataacta atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc
     7081 gcaacttaaa atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat
     7141 tgtaggctcg atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg
     7201 attttttaat gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac
     7261 gatggggatt ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga
     7321 acgagatgaa caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc
     7381 attctttatg tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc
     7441 tatcaatgag acagaaactg tagtaaatgg tattaggatg ggctcttttg tcgaacgttt
     7501 aggaacttca ggaatcttta cagctattat tatgtcgatt attgcggtcg aattgtattg
     7561 tatgtgtgtc aagagaaatt gggtcataaa aatgccagat gttgttccgc caggagtttc
     7621 tcgttctttc actgctctga tcccaacatt tgtcattgcg tttgtggtga tgatcatcaa
     7681 tggattatta gttgcactag gaacggatat cttcaaaatg attgctatcc catttgggtt
     7741 tgttacgcag ttgacgaata catggcttgg tgtcatggtg atttatttct tgatccatgc
     7801 attgtggatc gtagggatcc atggagcgaa tatcatcact tctttcttga ccccgatcgt
     7861 attagctaat atggcagcta acgcacaagg cgcaaattat ccgttggcag gagaattcaa
     7921 caattcttat gtaaccgttg gtggatcagg tgcgacttta ggattgatca tctttattgc
     7981 attcctcgcg aaatctgatc agctgaaagt gttaggaaaa gcctcacttg taccaggatt
     8041 attcaatatc aatgaaccaa tcattttcgg gatgccaatc gtctacaatc cctatttagc
     8101 ggttccgttt ttcttagctc cgatggcttc tgctacactc gcttattttg ccattcggct
     8161 ggaaatcgtc aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg ggatcggagc
     8221 atttgttggt agtggtggtg attggaaagc tgctgtgtta gcagtcattt gtgcaatcat
     8281 cgcattcatt atctggttcc catttatcaa attctatgat aataaattat taaatgaaga
     8341 aagagaaaag gcagcagaat tagctagtga aaagttaaaa tgatttctaa gaaaaaagga
     8401 actgtgacaa aaaatcacag tccctttttt tcttagaaat gggaaaccac tagtatataa
     8461 ataccattat tcttcaatca accgcttcac gattgcataa tccccttcat aaggttcatc
     8521 tacaccattg attttttgat agggatccac gccttttccc gcaataacga ctgaatcttg
     8581 cggagcgctt tctgctaaag cgcgtcgaat cgcttcagga cgatccattt cataatgaac
     8641 gatcaaatct ggattagtaa ttgcttcatt gatttcttcc gcgatttttt tcggatcttc
     8701 aaatgctggg tcatctgccg tcaaaaaggc gatgtcagct gtttctgata gcacttttcc
     8761 aaaatcatgt cgacgggaga ttgctttgtt tcctgggctg cctaatacaa caattacacg
     8821 gccatctgga tgttcatttt tggcaaattt cagcaatgtt ttcaagctta gataattatg
     8881 tgcatagtcc acatagacat gcgctccatt ggattggatc aattgatcca ttcggcctgg
     8941 tacacgagct tcttttaatc cgtcttgtgc atcttgactg gaggcacctg ttagtgctgc
     9001 ggcaatgatc gcactggctg cattttcttg gttgaatcct ccagccagca aaatatcata
     9061 agcagtatcg cagatagcta agcgatcttc atgagaagat aaagtaaaac cgtgagtacc
     9121 tttatctgaa cggatcactt gataattgct ttcttccgaa cgaccataag tgatcgttgg
     9181 aatctggaat agatcggctg tttccctaag taaatcaaaa tagtcacttt catgatttaa
     9241 aacaactgtt tttgaattca agatcaattg tcgcttgcaa tagaaatagt catcaaatgt
     9301 cggatgttcg atcggactga tatggtcagg cgagatattc aagaagattc cgacatctaa
     9361 agtcaagccg tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca tcactagatg
     9421 agtcatacca ttttcaactg cttctgacat catacgatat aaatctaatg actcaggcgt
     9481 tgtcaaatgg gatttgaaat atgttttgcc atctaacgtt gtattcatag tagaaagcaa
     9541 tgctgttttt ttatttgttg tgtgatccaa gattgctttt gtaaagtacg cagcagttgt
     9601 tttacctttt gttccagtga atccgatcac tttcaattta ttttgcggat agtcgtaaaa
     9661 agccatagca agaagcgcca ttgctttacg gatatccgtt acgatgattc ctactgctga
     9721 attttcgtat ggttgttctg atacataata ttgaataccc tgttcgattg ctgaatccaa
     9781 atattcttct ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat cagcttttct
     9841 tgaatcataa cttaactgaa gaaattcagc tttttctggt acatcaagat gccagcctct
     9901 tgttgaaaca aattctttta atagattttc ttttaatagt aaatgacgga tgtcttctaa
     9961 agagaagctc attgattgat ccctcgcttt attcaatttc ttggtttatt atagcacact
    10021 ctttttaata tatcgtttgt ttccgctatg acaaaatcca gctttattca aattttcttg
    10081 acacagggga caagcggatg agtattttca taaacaatga actgactgta atagatagga
    10141 gaacgaatag tgtctccaac aatccaaaaa cataataatt caaaaagacg ataagactat
    10201 tggtaagtaa aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa ataatcggcg
    10261 gtatcgttat accgccattt gaggccccga ctgtgtacaa tgtgtaaacc ccaagactga
    10321 acaaaagact tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc acttttggaa
    10381 caatctctaa aacaacagga aaaagcagac ttcccgctac agtcttaaaa aatactgatt
    10441 ttcctaatag gacagcggct agaagcagca tcataagatt gatgctccaa acagtaactg
    10501 agcgttggat agaaaaggcc gtttcaacta aatgtccgat ggcgcccact ccggcggaag
    10561 ctatatcgtg agggagcaag aaccaattca atccaactgt gataaagaaa agacctaaaa
    10621 atataacggg tagttttttt atttcttgtg tcattgatat ctcctataaa tgttagccat
    10681 aaaaggctgt atatttcttt actttaccaa aaaaacgaaa ccgaattcaa aaaaacaatt
    10741 gttcttataa aagttatata aaatcgattg ccagtttctg ttttaccaat taaaatgata
    10801 gtaatgtttt gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa ccagcattag
    10861 atcaattaac accgcaagaa aaaatggcac gaggctctgc ttggatgacc gccagtaata
    10921 tcatttcaag attgctaggc gctgtctata tcattccctg gtatgcttgg atgggcgaaa
    10981 atgctaaggc tgccaatgga ttattcaata tgggatacaa tatatatgca ttgtttctac
    11041 tgatctctac agcaggaata cctgctgcga ttgcaaaaca aactgcacgc tataattcat
    11101 tgaatgaata tggcacttca cgacgattat ttatccgtgc attacagatg atgggcgggt
    11161 taggcctgtt gtttgctttg ttcatgtaca tcgcctctcc ttggctagct catgcttctg
    11221 gtggcggaga agaactgatt ccaacgatgc gttctttgag tatcgctgta ttggtttttc
    11281 cttgtatgag tgtgatacga gggtatttgc aaggcaatca agaaatgatg ccttatgcgc
    11341 tgtcgcagat tgtagaacag gtggcacgta ttttttatat gctattggct acattcatca
    11401 tcatgaaagt catggatggt gactatgtga cggcggtcac gcaatcaact ttcgcggcat
    11461 ttatcggtat gctagcaagt attcttgtcc ttttgtattt tttgaagaaa catcaagcgt
    11521 atacatcggc gtttattcac tacagcgaaa acaaaataaa tattgcaacg aaggaattgt
    11581 tgttagatac cgttaaagaa gcgattccgt ttattattgt gggttcaggg gtaacgatct
    11641 tcaaattagt cgatcagttc acttttatga agatcatgtc tgcatccacc gagtattcca
    11701 atgcacagtt actggatctt ttttcgattt ttagtgccaa tccagataag ttgacgatgg
    11761 tagttattgc attggcaaca tcgatcgcgt ctactgggtt accgttgatt acagaagctg
    11821 ttacgttgaa agatcgagta ggtctggcaa aattgacaag cggaaattta cagttgtttt
    11881 cattttttat gtttccggcg acatttggtg ttatgttact tgcctatccg ctaaatacat
    11941 tattttatac accggattct ctaggaagta atgtactgat ccaagcaagt tttgtaggac
    12001 tcttcctagg actatatatg ctagtttcca acatgctgca aggaatgttt gaaaacaagg
    12061 cggcaattaa ttatttggtt attgggtttc tagtgaaact ccttcttcaa tacccagcca
    12121 ttcgtttatt tgaagtatac ggccctttat tggcgacaat gatcggattt gctgtgtctt
    12181 gtaccttgat cttaaagaga atccatgaag tagcccgctt taaaccaagt tttgtctggc
    12241 gcaggacatt gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa
    12301 agatgatttt tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc
    12361 ttgtagtagc aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg
    12421 cagaaagact actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag
    12481 tgaatcagaa gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata
    12541 agtttttagc agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag
    12601 gaacgatcga agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg
    12661 aagatgcggt tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt
    12721 tgcataaacc tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg
    12781 atttattgga agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag
    12841 acacagaggg cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac
    12901 ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag
    12961 atatccgttt atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa
    13021 aactccaaat tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc
    13081 atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat
    13141 atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat
    13201 atcgacccat gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc
    13261 taaaagaata agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt
    13321 tcggatggct tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg
    13381 tttgttcgga ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga
    13441 ctcagagtta gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca
    13501 caacctctga taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
    13561 aatatgagga actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca
    13621 caacctctcc tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg
    13681 aaagcgctat aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag
    13741 gggttcaatc atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta
    13801 ccgggtatgc tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca
    13861 ttcaaaaatt ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg
    13921 gtgggttggg aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa
    13981 aatacaattt tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt
    14041 gcaatcatta tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc
    14101 ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt
    14161 cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg
    14221 tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt
    14281 ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa
    14341 gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa
    14401 cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat
    14461 tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt
    14521 ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct
    14581 gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc
    14641 cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag
    14701 tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga
    14761 ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta
    14821 ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat
    14881 ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca
    14941 ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt
    15001 atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta
    15061 cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt
    15121 taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg
    15181 tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg
    15241 atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta
    15301 ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac
    15361 tatacatcca agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta
    15421 atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct
    15481 cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga
    15541 tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag
    15601 aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag
    15661 aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt
    15721 tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc
    15781 ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa
    15841 gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg
    15901 aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc
    15961 aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa
    16021 gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat
    16081 gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta
    16141 ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc
    16201 ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc
    16261 aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg
    16321 gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta
    16381 cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat
    16441 aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat
    16501 gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt
    16561 actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact
    16621 tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta
    16681 ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat
    16741 gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca
    16801 ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt
    16861 gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac
    16921 agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt agatggattg
    16981 aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag
    17041 gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa
    17101 taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga
    17161 ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga
    17221 aagccatatt cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct
    17281 tggtaaacgg tgttcaatca gctaaccctc ggtattcgct cacaaaacga caaaatatga
    17341 ggaactattt ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt
    17401 tttttgtata atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg
    17461 ttttggaaga gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga
    17521 tatatacatt tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg
    17581 aactatttta tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata
    17641 tatataaaag aaatttttta agaggcaatc tgctatttag tttattttta tctggtactg
    17701 ctttattgag ttacaattta tatttatctg ttcaaatcaa agggctcatt tttttagcta
    17761 ttgattttat tttacttttt gccttgctct gcctatttgc tacttatcaa tacagtatga
    17821 ttttagatag tgaatatacg atctctgttc ctaatctgct aaagctaagt tttatttcgg
    17881 ttttttccag cttttcgtct tttctaaaaa taattattgg tagcggtata attttaggcg
    17941 tgacttggca attcaaagga ttgatcctgt ttggtgtcat tggtcttttg acggtttgga
    18001 atggtacgat gacaacacat tggcgtgaag aattagataa acagttggaa agttatgaat
    18061 aagtttcgga ttctattttc aaaaggaacc ttactaaatc gtctgttgcg gaaatacgcg
    18121 gttatcatga tcggtgtaac gatgactgct accgttattt ttagtatcca tacttgggaa
    18181 caaaatcaaa aacaggcaga aaacatgaca agtgatgccg ttcaaagcac aagcagaatg
    18241 ctgaatgata aaacaacctt gagtaggatc atcaaaaacc agcttgtagg gaattccgaa
    18301 aagatcgaaa atgtgacgac ctacttaaca aaacccatag atcagtatct gatgtatgta
    18361 tacgaacagc aaaacagtac cgatgaatta gtttctttcc caaatcaaat caaagatctc
    18421 tacgcaaatt atgaagaact aagtgcgata tatatcgtgt tgaatcaact accggagtat
    18481 tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa aggggacacc aaaattacta
    18541 gacgcctttt atatcaagtt gcctatcaca caaggaggag ctgagtcagg aagtattttt
    18601 attggttttc aaaagaaaga actagatatg attttagaaa atctaacgtc attcaatgga
    18661 ctctctttat acatgatttc tggaacgaca aatcgactat acacattcca tgataataaa
    18721 atagctaaag aaacgtttga aagacaagaa atgatcatca cagagagtct gaagaaaaat
    18781 tcacaattgc cgattgacga tctggaaaaa aacaattttg ttcaacacca agaactgtct
    18841 gatgattatc gtatattagc tgtactagat aaaaaaagtg taaaaatcga aacgggaaaa
    18901 aatcttgccc cgctgatcat tggtatcatt attttagaca atatactgct tatctttctt
    18961 tataaaacat tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc catggatcaa
    19021 acagcagcag ggaatttaga gacgcggatc gacataacaa acacggaata tgaactgaag
    19081 gaactttcaa tcggtatcaa cgaaatgttg gatagcatca atcagtttgt agaagacatc
    19141 tacaaattag aaatcaaaca gcaagacgca catatgcggg cgcttcaagc acagatcaat
    19201 ccacactttt tatataatac attagaatat atccgaatgt atgcaatcag cgaaggaagt
    19261 gaagaattag cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa tacgaatcaa
    19321 gagaaaacga tcactttaaa agaagaactg gacttttgcg aaaaatatgt ctatttgtat
    19381 caaatgcgtt atcctaatcg agtagcctat cattttatga tcgatcctga tttggaaaag
    19441 atcgaagtac ctaaatttgt gatccagcca ttagttgaaa attattttaa acacggtatt
    19501 gattttactc gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg caaacgggta
    19561 aggatcatca tcaaagataa tggaaaaggg atgactgaaa aacggctaaa acagattgaa
    19621 gaaaaactgt ctcatcctaa agtggaactt cacggttcta tcggtctgca aaacgtgaat
    19681 gaacgtttac gtgccagctt cggcagcagt tattatatgt cgcttgagaa taatgagaca
    19741 ggtggactga ccgtttctat tacattcaag gagggatgag cgtgtatcaa gtgttattgg
    19801 tagatgatga atacatgatt ttgaatgggt taaaaaagat aattgattgg caaagtttag
    19861 gctttcaaat agtagcaaca gcagagaatg ccaaagaagg actagctgta cttgaacagc
    19921 gacagattga tttagtagta acggatgtga caatgccaga aataaatgga ctggaattta
    19981 ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat gattttatca ggataccaag
    20041 aattcgatta tttgaaagga ggtatgcaat taggcgccgt gaattatttg atgaaacccg
    20101 tgaataagtt cgaattggtt gaaagcctca agaaaatcaa aacacggtta gatcagcaaa
    20161 atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt attcagtcaa tggttgaatg
    20221 aagaattgga tgaaataagt gaagaagaaa taataggaca aataagtgag aagcagcata
    20281 ggatagtatt gattcaactg gcaagaactc atgatactct tatcaataga tggttgcaag
    20341 aacggcaaca aatcttttac tatcaacgaa attacggaga ccgtgtattg atcacgttgt
    20401 tgttggaggc agatgaagtt gatggcttct gttgttttgt tcaagaaaac tttcctgatc
    20461 aggaatggct gatcagtatc ggggaagaga cagattcttt ggacaaaatc cctgaaacat
    20521 tccaacaagc gaaagacaat ctacagttgc atcaatttta tggagataaa gacgagcatg
    20581 ttttttacgc agtacaagca gaaacaaaag atcaaaccat cgatttttca agtttcagac
    20641 gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat ttttttgaac
    20701 agtttcagtt agcagccatg atgccggaag atatacgata ttccagtttt ttactgttta
    20761 tggagattca acgggaattg attgatctgg aagatgagga atatttacaa ggaatcgaaa
    20821 aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca ttcatcaaaa
    20881 agcatcagag acagaaaaag tacagtgaca atgtggaaaa agtaatcgta atcctgcacc
    20941 agcattatca agaaccactt acactcaaag aagtgtcaga aaacctgcat ttaaatgtca
    21001 tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct tatttgaacc
    21061 atttacgtat ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac atcaatgaga
    21121 tagcaagtga gattggctac aacaacacca cgtatttctc taaattattc aagaaaatcg
    21181 tcggcagatc ccctaaagag taccgagaaa acagtggaga atcataaaag aaaaagccaa
    21241 tgtaatgaaa taacttataa aaaattgtct agaaatctaa ttttgggctg ttcttattac
    21301 ttgaggcaag gcagcttttt catgacctct cgataaacgg agttcaaaag ccagtttctc
    21361 ggtattaagc caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttaat
    21421 actcgaaact aaacagcttt ttcacgacct ctccactaaa cggtgttcaa tcagctgacc
    21481 ctcggtatta gctcaaaaaa tggcaaaata tgaggaacta ttttgccatt tgtctctcta
    21541 atactcaggt cagaacgctg atttcacaac ctctcaataa acggtgttca aaagcttgca
    21601 tctgcggtaa taagaccaac caaacgaaaa atatgaggat ctatttttgc ttggctgttc
    21661 ttattacttg atgcaggaca gctttttcac aacctcttgt taattatagt ttgataagga
    21721 aaaaggcaaa tcttttgctt gatatttctt agggtgaggg atgatcccta attggtaaga
    21781 gatcgtgccg cctttcagca aatcttgatg ggtaaaatat aatttcgtat attctttgtc
    21841 catccgtttg attttatgga taaattgttg ctgcggccga ttaggcgttg cttcaatcgt
    21901 caattccttt ccatttgaca aaagcaaaac agccttgtca attaatggca taccgataac
    21961 atattcaccg gttccaggcg tgacaggata aaatcccaga ctattgaaga tataccaagc
    22021 ggccatactt ccattgtcct catctccagg gtaacccatt ggagaatcgt cgaaggtctg
    22081 agtcatcaac tgtttaagca gtggctgtgc catttcaggt tgtcctatat aactgaacaa
    22141 gtaaggatag tgaaaacttg gctggtttga aatagctaat tgtccaaatt ctatcgcggc
    22201 catctcactc atttcgtgga tctcaaaacc atatcctcct gggtcaaacg ttgggggctg
    22261 attgcacagc tcaatgagtt tttctttata tctctcactg ccaccatatt ttccgatcag
    22321 tcctgaaaaa tcgtgataaa cagcaaagct gctttgccaa gcacttcctt ctgcataatc
    22381 ttgtccccaa cgattagctg aaaatggttc tctgaacttg ccatctttat cttttgaacg
    22441 cataaatcct gactcatagt caaagatatt ttgatagttc aatgattgct gctgatattt
    22501 cttcgtcatt tcctcttctt ctagtacctt ggcaacttgg ctaatacaaa aatcgctata
    22561 agcatagtct aatgtgtgat tgactgattc gtggtagtga ttaggaacat agccatatgt
    22621 caagtaatct tctgttcctt ggcgtccata atttggatgt ttgctttgaa ccgtagcacc
    22681 tttttccatt gccgttagaa attcaggcat caagtctcgt cggatatctt ttacagcagc
    22741 atctgcaatg accgcatcga tcaatgtccc aggcataagt ccacgttcat caggagacag
    22801 ccattttggc aaatagccac tttctctata gctgttcaaa aaaccttcca gcatttcttc
    22861 gtatttttct acagcaatca aagaataaag aggataaacc gttttatacg tatcccaaaa
    22921 cccattattt gtatagagaa caccaggttt tatagatcga ctagtcgtat catagtggat
    22981 tttttgccca ctaggatcaa tttcataaaa tgtttgtgga aataaaaaca cccgatataa
    23041 attatgataa aacgtccgca attgttgttg atcatggtgt gtgagctgta tacgatcaaa
    23101 ataggactgc cattgttctt gactgtgagc aaggtaagtc tctttgcttt ggttttgttc
    23161 tagttccagg ttataagctg cttgttccga gctgataaaa gatgtcccaa atcggatagt
    23221 ttgttttttt gtctgttcaa aatacaacgg gatcgagtcg ctatttttgt tggaaacagc
    23281 taactcatca attgttaaag gatattcaaa tctcaatata aaataaaaag aaaaatcagg
    23341 atcttctgaa ccagcaaaat gtgtgatttt tccttccact gtgtaaggat ctataacaaa
    23401 gagctgatgg cgtcccggaa aggataaaag taaaccgctg tcatttttgc tgtaatcaat
    23461 agataagatt ccgccataca tgctaggtat caatgtagaa cagatgccat aacgtaattg
    23521 acagatcgac agatgagtcg ggcaaaatat actttcttct gggcggtaag aactttgagc
    23581 atggaaaagg gtgttctcct gtagttttcc attgatcggc gtaaacagca tatgactgaa
    23641 atcacccatc caaggacttg gttgatgggt aagacggtag ccttgaaaaa cgcggtcatc
    23701 aggatgaaac caccagctgc cgttttgatc cgtagtttgc ggtgcaaaat aattcatccc
    23761 aaaaggaacg ccagtataag gctggcagtt cccattagag aaatttggct gattcgctgt
    23821 tgcatgtcta gtatctattt gaccgatatt cattttattt ctcctttgtt gattgtcttt
    23881 tgatgaatga aactggaaat ttactatcgg atggtcgatc ttttgtctct aaccaagtga
    23941 ccaaagcatc tcccgcaagc ttacctaact gttcaaaatc ttgtgcgatg gttgtcagtg
    24001 gcgggtcaac aagagctgct gcttggatat catcaaatcc gataattgaa aaatcttcag
    24061 ggatatgata accatgctgt ctcaagagat tcatggtctg gatagcgaca acatcgtttt
    24121 cacaaacaaa agcagttact tcatacttag tgatataaga aagcaattca tctaatgtcg
    24181 cgcttttgtc atccagtgaa gtgtggaaag tcaaaccagc tttgtccaat gcttcaaggt
    24241 aaccaaggta acgctgtcga acggattgtg gatgagtctg cgtaccaaac aaataagcga
    24301 tttttgaatg tccctgttcg atcaaataag aggcagcaag cgcaccgcct tggaaattat
    24361 cggaaagaac ggtaggaaaa ggtaactcat agattttttt atctaatatg acgacaggga
    24421 attctttcaa tgaaagctca aaaagtaagt caagatgttg ttctgtatca agagcataat
    24481 aaatcaatcc gtcaaattca ttgattagct cttttgctgt tttattttgt agaaagtcaa
    24541 gcgtcgtcat catgacatca atctgtttat cttgcatcac agggttcaat cccttgctaa
    24601 aatcaccaac agacaaatca gcgacatatg gaagaagaaa cagaatccgt cgtgtttttg
    24661 taggagaagc aagaatctgt tccggagaag cggatttaac gaaactgcct ttcccgcgaa
    24721 cgcgataaat aagtcctaat tgctctaatt cagtcaatgc acgtttggaa gtgattctgc
    24781 ttactttata ggtacgtgaa agttcttttt cagttggtag ttggctgtct acaggtaatt
    24841 caccagttaa aatagccgtt tgcaaatcat taaatattgt ctggtacaat ggttgattca
    24901 tataaacacc tcttaatgat atatcatatt caaataatac cataatggat gaaaaagtaa
    24961 ataaaaagat attttttgaa agcgtagaca taattggctg tatgaagtat aatctttaaa
    25021 gatatatcaa atagaaagaa ggttttatta gtggcttata aagatatacc aaaatcggtg
    25081 cagaaattta tggaagatat cacacaaaaa tgtggggata tccatgcgga ctgggcaaag
    25141 aattttacag cagcatttgc caatactctg ttgacaacag tcaaaaagca aaatgacgga
    25201 accacctttt tattgacggg agacattcca gcgatgtggt taagagactc tactgcacaa
    25261 gttcgtcctt atttagtgat tgcaaaagaa gatgaggaat tagctgacat gatcagcggg
    25321 ttagtcaaaa agcaattttt ttatatcaat atcgatccat atgcgaatgc cttcaacgaa
    25381 gaagctaacg gtgctggcca ccaaacagat catacaaaaa tgaacgattg gatctgggaa
    25441 agaaaatatg aaattgattc tttatgctac ccaatacagt tggcctatct tctttacaaa
    25501 aatacaggaa aaactgatca attcaatcaa tcttttattg aaggtatcaa aaaaatcttg
    25561 gacgtgttca aaacagagca agatcatgaa cactctcctt atacatttgt acgagacacg
    25621 acaagaaaag aagatacgct gatcaatgac gggcgaggtg caaaaacagc ttttacagga
    25681 atgacatggt ctggtttccg tcctagcgat gatacttgcc gttatggata tctggtacca
    25741 tccaatatgt ttgcggtggt cgtgttaggt tatgtggaag aaatttttca aacgatactc
    25801 gatgataaag aaattgtaca gcaaacacag aaattgaaga aagagatcca agaggggatt
    25861 cagaatcacg gacgaacggt taatcaaaaa gggcaaacga tttatgcata tgaagtagat
    25921 ggaatgggaa acgccacagt catggatgac agtaatgtgc ccaatctgat cgccgcacct
    25981 tacttgggat attgttctga acaagaggaa gattatctgc aaacaagaag aacattgttg
    26041 agtaaagaaa atccatattt ctatgaagga aagtacgcaa aaggaattgg atcctcccat
    26101 acgccagaaa aatacgtttg gccgattgca ttagctatgg aaggaatgac aacgtcagac
    26161 aaatctgaaa aagagcggat attgaattta cttgtagcaa ctgacgcagg aacacacctt
    26221 atgcacgaag gattcgatgt gaacaatcct gaaaattata cgagagaatg gttttcttgg
    26281 gcaaatatga tgttttgcga attagtcatg gattactttg atattagagt agaaaagtag
    26341 gaggtatcgt aaatgaaaaa gaaagtatat atcatttctc atagccactg ggatcgtgaa
    26401 tggtacatgg cttacgaaca gcatcacatg cgactgattg aattgataga cgatctacta
    26461 gagctatttg aagttgatcc gtccttcaac agttttcatt tggatgggca aacgattatt
    26521 ttagatgact atcttcaagt aaggccagaa aaaagacagg caatccaaca agcaatcaat
    26581 gaaggaaaat tgagaattgg accattttat atcttgcaag atgattttct tatcagctcg
    26641 gaatcgaacg tgagaaatat gctgattggc atggaagaaa gccgtaaatg gggaacccct
    26701 gtcatgttag gctattttcc cgatacattt ggaaatatgg gacagacgcc tcaattaatg
    26761 aaacaagctg gtatttcagc agcagcgttt ggccgtggtg taaaaccaat tggctttgat
    26821 aatcaagttt tggaagcaga aaactattct tcccaatact cagagatgtg gtggaaagga
    26881 cctgatcaaa cagcaatctt tggtctgcta tttgccaatt ggtatagcaa cgggaatgag
    26941 attccagtag aaaaagaagc agctcttgcc ttttggaagc aaaaattagc ggatgcagaa
    27001 caatatgcct ctacgaacca cttattgatg atgaatggtg ttgatcatca accggtacaa
    27061 aaagatatat caaaagcaat ccatctcgca aatgaactgt ttccagatta cgagtttatt
    27121 cattctaatt ttactgacta tctggaagca gttcaaaaag atgtgccaga agacttaggc
    27181 tcagtagaag gagaattgac aagtcaagaa acggatggct ggtatacttt agccaataca
    27241 gcatctgctc gggtttactt aaaacaatgg aataccaagg tccagcgaca gctagaaaat
    27301 attacagaac cacttgcgac gatggcttat gaagttagtg gaaactatcc ccatgatcaa
    27361 ttagattatg cttggaagac attaatgcaa aatcatcctc acgacagtat ttgcggttgt
    27421 tcagtcgatt ctgtccatcg ggaaatgata cctcgttttg aaaaagcaga tgaagttgga
    27481 aagtatcttg cacaagattc cttagaacag ctgaccgcag caatcgatac gaccggattc
    27541 ccaaaagaca gctttccatt tgtcattgta aatactgcag gaatggataa aactggtgaa
    27601 gcagaaataa ccatcgaatt agaaagaaaa agattcgctg aaggaatacc tgaacaacta
    27661 tatcaagagt tagaaaattt gcctaaacgt aagtatcatg tagaaacaaa aagtggggcc
    27721 actataccag ctattttatc tgaagaaact gtccaatttg gttatgattt gccgaaagac
    27781 cgtttccgtg tgccatatat ggcaagaatg atcaaagtga ctttaccatt agaaaacatg
    27841 ccagcatttt cttgggaaac attcgcgctt gtagaagggg aagcgaaagc agaagaaaag
    27901 gaaacaatga ttcatcagag tggacgaatc atcgaaaacg gccctctaca tttgacgatt
    27961 gaaaaaaatg gtaccattac aatggaagat cgcaaaaaca aacgaaagtt gaataatctg
    28021 catatttttg aagatatcgg cgatatagga aatgagtata tctttaaaca acctttctgt
    28081 gatcagccaa tcctatccag taacaaagaa aactctgaag taaaagtatt agtagataca
    28141 ccggaaatag caaaaataag tattctacag gaaatggaaa tccctgtttc tgcagacgag
    28201 cgtttagaaa aagaacagca gatggttgtg gaatttcgtt atcgaaaagc agaacgatcc
    28261 aaagaaaaac gaatccttca aataaaaaca atcatgacaa tcagaaaaga tagtaaaaaa
    28321 atcgactttg aaacgactct cgacaatcag atgaaagatc atcgcttacg tgtcttgttt
    28381 cctacaaagt tacatgtgga acatcacgaa gcagatagta tttttgaagt ggtgaagcgc
    28441 ccaaaccatg tgtctaaaag ttgggaaaac cccacgaatc cgcaacatca gcaagcattt
    28501 gtcaatattc atgatgaaga gtacggtgta acagtcggaa actttggttt gaatgaatat
    28561 gaagtaacag aagacggaca aatcgcagtg acgctacttc gcagtgtggg agaactaggc
    28621 gattggggat attttccaac accagaagca cagtgtctag gtgagcatcg gttcaattac
    28681 agcattgaac tacatggacc agaagaaaaa ttctctactt atttgcatgc atacgcagca
    28741 cagattcctt tttccactca acaaatcaaa catcatgaag gaacattgat ttcaaaacaa
    28801 caatatttaa cgataaaaag tgaaacgttt gcaattacag ctttgaaacg cagcaagttt
    28861 agtgataaag tagtagtaag aggtttcaat atgtctagtc atttagaaaa attggaaata
    28921 actaaagaca atggaaaaac tgtcatattg aatctgcttg aagaaccaac gaagcaagcg
    28981 gttgtcccaa taatccaacc ttatgagatt cggacaatcg gattcgagga ggaaaacgaa
    29041 tgtatggcgg aatagaagca ggcggaacaa aatttgtgtg tgcggtttca gatggtggag
    29101 aaattttaga aacgccgatt gtaaaattaa gctagacaac taaaaaagtc atttgtgcta
    29161 cactcaaaat agttccgacc aaagaattat aaggagtgag tacaaatgac ctatacccat
    29221 cttacaacgg atgaacttgt aataatagag tcttatttca aaatgaatca atctgttgct
    29281 aaaactgccc attgcttgaa tcgttcaaga cagacgatcc ataaagtata tctgttcttc
    29341 aagcaaggaa aatcagcctt agaatattat caacagtata agaaaaacaa atcaaactgt
    29401 ggtagacgtc cgcttgtttt acccgaggaa caatcagaat atattcaaag aaaggttgtt
    29461 caaggatgga cacccgatgt gattgttggt cgtgcagcgt ttcctatt
//
LOCUS       DAHUVV010000023        28116 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.023, whole genome shotgun sequence.
ACCESSION   DAHUVV010000023 DAHUVV010000000
VERSION     DAHUVV010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.023"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            49..291
                     /gene="yaaA"
                     /locus_tag="NK767_001080"
     CDS             49..291
                     /gene="yaaA"
                     /locus_tag="NK767_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCH6694088.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            278..1402
                     /gene="recF"
                     /locus_tag="NK767_001081"
     CDS             278..1402
                     /gene="recF"
                     /locus_tag="NK767_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCH6694089.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1399..3345
                     /gene="gyrB"
                     /locus_tag="NK767_001082"
     CDS             1399..3345
                     /gene="gyrB"
                     /locus_tag="NK767_001082"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCH6694090.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3365..5836
                     /gene="gyrA"
                     /locus_tag="NK767_001083"
     CDS             3365..5836
                     /gene="gyrA"
                     /locus_tag="NK767_001083"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCH6694091.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6072..6371
                     /locus_tag="NK767_001084"
     CDS             6072..6371
                     /locus_tag="NK767_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCH6694092.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6421..6933
                     /gene="ssb"
                     /locus_tag="NK767_001085"
     CDS             6421..6933
                     /gene="ssb"
                     /locus_tag="NK767_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCH6694093.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6959..7195
                     /locus_tag="NK767_001086"
     CDS             6959..7195
                     /locus_tag="NK767_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCH6694094.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7338..9311
                     /locus_tag="NK767_001087"
     CDS             7338..9311
                     /locus_tag="NK767_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCH6694095.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9317..9769
                     /gene="rplI"
                     /locus_tag="NK767_001088"
     CDS             9317..9769
                     /gene="rplI"
                     /locus_tag="NK767_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCH6694096.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10043..11410
                     /gene="dnaB"
                     /locus_tag="NK767_001089"
     CDS             10043..11410
                     /gene="dnaB"
                     /locus_tag="NK767_001089"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCH6694097.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12190..13482
                     /locus_tag="NK767_001090"
     CDS             12190..13482
                     /locus_tag="NK767_001090"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCH6694098.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            14269..15132
                     /locus_tag="NK767_001091"
     CDS             14269..15132
                     /locus_tag="NK767_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCH6694099.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(15261..16883)
                     /locus_tag="NK767_001092"
     CDS             complement(15261..16883)
                     /locus_tag="NK767_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6694100.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            17132..17653
                     /locus_tag="NK767_001093"
     CDS             17132..17653
                     /locus_tag="NK767_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCH6694101.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(17839..19569)
                     /gene="spxB"
                     /locus_tag="NK767_001094"
     CDS             complement(17839..19569)
                     /gene="spxB"
                     /locus_tag="NK767_001094"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCH6694102.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            19734..20552
                     /locus_tag="NK767_001095"
     CDS             19734..20552
                     /locus_tag="NK767_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HCH6694103.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(20655..20852)
                     /locus_tag="NK767_001096"
     CDS             complement(20655..20852)
                     /locus_tag="NK767_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694104.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            21021..21599
                     /locus_tag="NK767_001097"
     CDS             21021..21599
                     /locus_tag="NK767_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCH6694105.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            21694..21831
                     /locus_tag="NK767_001098"
     CDS             21694..21831
                     /locus_tag="NK767_001098"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694106.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(22644..23147)
                     /locus_tag="NK767_001099"
     CDS             complement(22644..23147)
                     /locus_tag="NK767_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCH6694107.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            23506..24531
                     /locus_tag="NK767_001100"
     CDS             23506..24531
                     /locus_tag="NK767_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCH6694108.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNLMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            24854..25732
                     /locus_tag="NK767_001101"
     CDS             24854..25732
                     /locus_tag="NK767_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HCH6694109.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            26208..26324
                     /locus_tag="NK767_001102"
     CDS             26208..26324
                     /locus_tag="NK767_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCH6694110.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            27252..27590
                     /locus_tag="NK767_001103"
     CDS             27252..27590
                     /locus_tag="NK767_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694111.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            27684..27914
                     /locus_tag="NK767_001104"
     CDS             27684..27914
                     /locus_tag="NK767_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694112.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         9134 a         4889 c         5766 g         8327 t
ORIGIN      
        1 aaaggtataa tagagtatac ttttaataat gaaaatgagg gatgggcatt gaaacaacaa
       61 gtcattctaa atacggaatt catgacgttg ggccaaatgt tgaaagaagt cacagttatc
      121 ggtagtggcg gacaagcaaa atggtatttg gcagaaaaca ctgtgttagt cgatggcgaa
      181 ccagaaaatc gtcgtggacg caaactttat cccggcatga tggttgaagt tccagaagta
      241 ggaacttttt ttatggtaaa aaaaggaaac gacgataatg aggctgaata agctgtattt
      301 aaaaaattat cgaaattacg aagagctgaa catcgaattt tccaagaacc tcgtcatctt
      361 tttaggagaa aatgcgcaag gaaaaacgaa tcttttggaa agtatctacg tattagctat
      421 gacgcgaagt catcggacaa gtagtgagca ggaattgatt ggctggactg acgatcaggc
      481 gatgattcaa ggagagatca caaaaggttc atccactctt ccattagaaa tcttattatc
      541 taaaaaaggc cgaaaaacca agatcaacca tatcgaacag aaaaagttaa gtacttacgt
      601 gggacaaatg aatgtcattt tatttgcacc agaagatctt tctttagtaa aaggcagccc
      661 gcagatcagg cggaagtttt tagatatgga attaggccag atcaatccaa tctatcttta
      721 tgatcttgtc caataccaag caattttgaa acaaagaaac cagtacttga agcaattgaa
      781 tgaaaaaaaa caaactgatt tgctttattt ggatattttg tccgaacaac tagcagcgtt
      841 tggcagcaaa gtattgaaag caagagcaca gttcgtcaag cgattggagt tttgggctaa
      901 ttctttgcat cagcaaatca cccatcaaaa agagcagctg gaaatcgagt atctcaccgc
      961 agtcgattct ttagaaacac atacgcaaga gcaaatccaa gaacaatttc tcgcactttt
     1021 gaaccaaaat aaaaagaaag acctttttcg gggaacaacg acagttggtc ctcatcgtga
     1081 cgatctttcc ttttttatta atcagaaaaa tgtgcagact tatggatcgc aaggacagca
     1141 aagaacaact gcactaagtg tcaaattagc agaaatcgat ttgattaaag aagagacagg
     1201 ggaatatcca atccttttgt tagatgatgt gatgagcgag ctggatgata atcgtcaact
     1261 ccatctgcta gaaacaatcg aaggaaaagt tcaaacgttc ttaacaacaa cgacacttga
     1321 ccatgtaaaa aataaaatga ctgttgaacc tgagattttt tatgttcaac aagggaaact
     1381 aaaaggagaa gaaactgaat gacagaagaa agaagtttag tagaacgcgc taaagagtat
     1441 gatgccagtc agattcaagt acttgaagga cttgaagccg ttcgtaaacg acctggcatg
     1501 tatatcggtt cgactagttc tgagggtctg catcaccttg tttgggaaat tgtcgataat
     1561 tcgatcgatg aagtattagc aggttttgca acgaaaatcc atgtgattat tgaaaaagac
     1621 aacagtatca ccgttattga cgacggtcgg gggatacctg tagatatcca ggcaaaaact
     1681 ggacgtcctg cagttgaaac ggtctttact gttttacatg ctggaggaaa attcggcggt
     1741 ggtgggtaca aagtttctgg tggacttcac ggagtaggat cttctgttgt aaacgcgctt
     1801 tctacacagc ttgatgtcaa agtatacaaa gaaggaaatg tgtactatca agaatatcgt
     1861 cgtggagctg tagtggacga cttaaagatt atcgaacaaa cggatcgcca tggaacaacg
     1921 gttcacttta cgccagaccc tgaaattttt acagagacaa ctgagtttga tttttcaaaa
     1981 ttggctaccc ggatccgtga acttgctttc ttgaaccgcg ggatgcgtat atcgattgaa
     2041 gacaaacgag aagaagaacc agtcatcaat gaataccatt atgacggcgg gatcaaaagt
     2101 tatgtcgaat acttgaatgc aaacaaaacc gtgatttttc cagaacctgt atatttggaa
     2161 ggtgaacagc aagatatcgc agttgaagtc tctatgcaat atacagacgg ctaccattcc
     2221 aatatcatga gttttgccaa caatatccat acgtatgaag gtggaacaca tgaatcagga
     2281 ttcaagactg ctttaactcg tgtgataaat gattatgccc gaaaacaaaa gctgatgaaa
     2341 gaaaatgacg acaacttgac cggtgaagac gtgcgagaag gattgacagc tgttatttct
     2401 atcaagcatc ctgaccctca attcgaagga caaacaaaaa caaaattggg caattccgaa
     2461 gtacggacag tcactgatcg gttatttagc gaacatttca tgaaattctt gctggaaaac
     2521 ccaagtgtgg gccggcaaat tgttgaaaaa ggattactag cttccagagc tcgtctagca
     2581 gcgaaacgtg cgcgtgaagt aacgagacgt aaaggtgctt tagagattag taatctgcca
     2641 ggaaaattag cggactgctc tagcaacgat cctgaaaaat gcgaattgtt catcgtcgaa
     2701 ggagattcgg ccggcggatc agctaagcaa ggtcgtaatc gagagtttca agccatcttg
     2761 cctattcgtg ggaaaatctt gaatgttgaa aaagcaagca tggacaagat cttggcaaat
     2821 gaagagatcc ggtctttatt tactgctatg ggtacagggt ttggagaaga ttttgatgta
     2881 tcgaaagctc gctaccacaa attagttatc atgactgatg ccgatgtcga cggcgcgcat
     2941 atccgtacat tgctgctgac tcttttttat cgttacatgc gtcctattgt tgaagctgga
     3001 tacgtctata ttgctcagcc acctttatac ggggtaaaac aagggaagaa tattacgtat
     3061 gtacaaccag gaaaaaatgc agaagaagag ttgaaacaag tcgttgcttc acttcctgcc
     3121 tcaccaaaac caagcgttca gcgttataaa ggacttggag aaatggatga tcatcaacta
     3181 tgggaaacaa ctatggaccc aagcaatcgg atgatggccc gcgtcagtgt ggacgatgct
     3241 attgcagcag atcagatatt tgaaatgttg atgggggatc gagtagaacc ccgtcgtgca
     3301 ttcatcgagg aaaatgcaca ctatgtgaag aacttagata tttaatcatg aaggaggaaa
     3361 aagaatgagt gaagaaatca gagaaaacat ccatgatgtc aatctaacca gcgaaatgaa
     3421 agactccttc atcgattatg cgatgagtgt tattgtcgca cgtgcacttc ctgatgtcag
     3481 agatggtctg aaacccgttc atcgccgtat tttatacggc atgaacgaat taggtgtaac
     3541 accggataaa gcccataaaa aatctgccag aatcgtcgga gatgtcatgg gtaaatacca
     3601 tcctcatgga gacattgcga tctatgagtc aatggtacgg atggcacagc cattcagcta
     3661 ccgttatatg ctagtggatg gtcatggaaa ctttggttct gttgacggtg acggtgccgc
     3721 cgctatgcgt tataccgaag cacggatgag caaaatcgct acagaaatgc tgcgtgatat
     3781 caataagaac actgttgatt tccaaagcaa ctatgatgat acagaaaaag aaccagttgt
     3841 actacctgct cgcttcccga atcttttagt gaacggaaca acggggatcg ccgtaggtat
     3901 ggccacgaat atcccacctc ataacttgtc tgaggtagta gcggctatcg atttgctgat
     3961 ggataatcct gatgtgacga ccaatgaatt gatggaagta ttacctggac cagattttcc
     4021 aacaggtggt ttggttatgg ggaaatcagg aatccgaaga gcgtatgaaa caggtaaggg
     4081 ttctattacc gtacgtgcaa aagttgagat tactgagatg ccaaatggta aagaacggat
     4141 catcgtgaca gaattgcctt atatggtcaa taaagcaaaa ttgatcgaac ggatttctga
     4201 actgcacaga gacaagcgta tcgaagggat cacagatttg cgggacgaat cttcccgtga
     4261 aggtatgcgg atcgtcattg acatccgtcg tgacgccagc gcatccgtta tcttgaataa
     4321 cttatataaa ttgacatcct tgcaaaattc atttggattc aatatgttgg cgatcgaaaa
     4381 aggtgtgccg aaagttttga gcttgaaaca aatccttgaa aactatatcg aacatcaacg
     4441 cgaagtcatc acacgacgga cggtctttga aaaagaaaaa gcagaagcaa gagcacacat
     4501 cttagaaggt ttgcgtattg ctttggatca tatcgatgaa atcattgcga ttatccgcgg
     4561 gtctaaatca gatgacgagg caaaagcaac aatgatcgaa cggttcgatt tatccgatcg
     4621 ccagtctcaa gctattttag atatgcgttt acgccgattg accggtttgg aacgggaaaa
     4681 aatcgaaaat gaataccaag aactcttaaa actgattgaa gatttaactg acattctggc
     4741 aagaccagaa cgtgttacag aaatcatcaa gaatgaatta gcagacttga atcaacgatt
     4801 tggtgataaa cgacgcacag aattactagt cggtgaggtc ttaagtctgg aagatgaaga
     4861 tttaatcgaa gaagaagaaa tcgttattac attgacaaac aatggttaca tcaaacgttt
     4921 ggcaaataat gaattcagag ctcaacgaag gggtggacgt ggtgtccaag gaatgggtat
     4981 ccacaatgac gacttcgtca aaaatctggt ttcttgttct acccatgata cattgttatt
     5041 ctttacgaat aacggaaaag tgtatcgggc aaaaggatac gaaattccag aatacgggcg
     5101 tacagccaaa ggaatcccaa tcatcaattt actaggtatc gattcgagtg aaagcatcca
     5161 ggcaatcatt gcggtgacgg gagaagcaga agaaggccac tatctcttct ttacgacgcg
     5221 tcaaggtaca gtaaaacgta cttctgtcac tgcttttgct aacatccgaa gtaatggtct
     5281 gatcgcaatt ggtttgaaag aagacgatga gctcgtcaat gtgctactga cagatggtca
     5341 atctaatatc attattggaa cacataacgg ctactctgtc actttcaaag aagaagcagt
     5401 ccgcgatatg ggacgtacag cttccggggt cagagggata cgcctgcgtg aggaagatta
     5461 tgtcgttggg gcagccttaa tgaaggaaaa tcaagaagta ttagtgatca cagaaaaagg
     5521 ttacggaaaa cggacaaaag tatcggaata tccagtgaaa ggccgcggag gtaaagggat
     5581 caaaacggcg aacatcactg aaaaaaatgg tccactagct ggattgacca ctgtttctgg
     5641 agatgaagac atcatgctga ttacagataa aggtgtcatt atccgtttca acgtatcgac
     5701 tgtttctcaa actggacgtt caacattagg cgtacgtttg atgaagatgg aagctgatac
     5761 gaaagttgtc acgatggcag cagtcgaacc agaagctgca gaacaaacag ttgaagaaca
     5821 aggatcagaa gaataaccat caagactaag tgtgtaagct agcaagaata accgattaaa
     5881 aaatctggtt attcctgctg gcttttttga ttagttgaat ttctcttaaa atattcgaaa
     5941 ctgttcattc tgtattgctt ttgaccaaaa tagagtgtat aattagagct tgtgagtagt
     6001 ctaattaatt agataactct ccttgctctt aggaacctaa gagccgaaag tccacaggga
     6061 ggtgaatatc aatggaaaac acgaaatatg aaatcatgta cattattcgt ccaaacattg
     6121 atgaagaagc aaaaactgct ttaatcgaac gtttcgatgc aatcttgaaa gataacggag
     6181 cagaagttat cgaatcaaaa gattgggaaa aacgccgttt cgcttatgaa atgaacggtt
     6241 accgtgaagg tatctatcac atcgttaaag tatcttctcc atcatcagca aatgctgtca
     6301 atgaatttga ccgtcttgct aaaatcaacg acgatatcat tcgtcacatg attgttaaag
     6361 aagaagctta attgttgttt catgtgaaac aatagaaaac gatgagagga gatgactatt
     6421 ttgattaata atgttgtatt agttggacgt ctgacgaaag atccagattt gcgttacaca
     6481 gcaagtggta cggcagtcgc aacatttact ttggctgtga atcgtaattt tacaaaccaa
     6541 agcggcaatc gcgaggctga ttttatcaac tgtgtgattt ggcgcaaatc agcagaaact
     6601 ttagcaaact atgctcgtaa aggaacactt ttaggtgtta ctggacgtat ccaaactcgt
     6661 tcttatgata atcagcaagg acaacgtgta tacgtgacag aagtagtagc tgacaacttc
     6721 caattgctgg aaagtcgttc tgcatctgaa aatcgccaac aaagcggcgg attccaaagt
     6781 tctggtcaat cagctggcgg atttggcgga aacaataact ctaatcaaac atctcaatca
     6841 tccaacggta tgcctgattt cgaccgtgat acttctgacc cattcggctc aagttcaacg
     6901 atcgacattt cagacgatga tttaccattc taaacgaatt tgataggagg gaaatagaat
     6961 ggcacaacaa agaagaggcg gacgtaaacg tcgtaaagta gactacattg ctgctaacca
     7021 tattgaatat atcgattata aagatatcga attactaaaa agatttattt cagaacgcgg
     7081 taaaatcttg ccacgtcgtg taactggtac aggcgctaaa aaccaacgta aaattactgt
     7141 tgcaatcaaa cgcgctcgta tcatgggctt gctacctttc gttagcgaag actaataaat
     7201 cctaggaatt gaatgactga tccttttgat cagctattca attccttttt ttctgtaaaa
     7261 ataacctcta ttcgtttagt cgctcgaatg acgaaagtta tggtaaaatc aaattagtca
     7321 tatatgggag gaaaattatg gaaaataaaa acaatcattt atccccgaaa cctctcttcc
     7381 ttatcctgtt tatattaatc gaagtattgg tagtcttaat tttaccattt cgttttgtta
     7441 gcgcttcggt cattctattg atgaatttat ggggtgttcg ctattggttg aaaatgaaac
     7501 gttggcttat tttaaataaa cgtgaaaaaa tccagcaggc ttcgactgta gctgaaacca
     7561 atctagctta tgtctcagaa gtcatgcctg taggagtgat tgcttatcag ccatcaagcc
     7621 aaaaaatcga atggctcaat ccattagcat tgaaaatcaa agatcaaagt ggattaacag
     7681 aggaagaatt gctagatact ttttttactg caaaagaaaa aaagaaaaac caagtctatc
     7741 tagcagataa aacatttcag tttgatatgg atacagtaaa gggtgttgtt tattttaatg
     7801 atattaccca aaaccagttg ttaaaactcc atcagacgaa cagtcagccg gtgattggta
     7861 ttttatctat tgataactat gcagatgcca ttgataaatt agatgacaaa gagatttctt
     7921 atttgaactc tttcgtcact acactagttt ctgactggat gaatgaatac catgtctttt
     7981 tcaaaagaat caacgcagaa cggtttttct tcgtagcaca gatgatcgat ctgaaaaaga
     8041 tgatggataa aaaatttgat ttgctggatc gctttcgagc agaagctgaa aaacagagct
     8101 cgcctatcac ccttagtatg gggattgctt atggctcaag aaccctcgat gaaatcggtg
     8161 gagaagctca gaataattta gatgttgctt tagtccgtgg aggcgaccaa gttgtcgtga
     8221 aagaggtaaa agaaggagca aaacctgttt attatggggg gaaatctgct aagaatgtca
     8281 aacgcacgcg tgttcgttct cgagcgatga gtaccgcttt gcgtcggatt tttgatgaaa
     8341 caggcgatgt gtttatcatg gggcacaagt ttccggatat ggatgcttta ggctctgctt
     8401 ttggtgtcag ttgtctggca cgcttcaacc agaaaaaagc ttacatcgtt atcaatgaga
     8461 acgagatcat tccagatgta gaacggtgtc ttgaagaaat ccataagcat cctgaattag
     8521 aaatgcaact aatctctcca gaacgtgcca tagagctgaa aaaagaagag gacctcctcg
     8581 tgatggtgga ttaccataaa ccttctctat cgatctctca gggcctatat gagcaatttg
     8641 ataagattgt gatcattgat caccatcgtc gtggggaaga gtttccaagt aaacctctgc
     8701 tgacttatat tgaatcttca gcttcttcag cttcagagct agtttctgag ctgatccaat
     8761 accaaagcag caaaacaaat cgcttagatc gttttgaggc cacattattg cttgcaggga
     8821 tggttgtaga tacaaatagt ttcaatgtcc gtacaactgc acgaactttt gacgttgcca
     8881 gctatttaag gtcttgtgga gcagacgcat ccttagtaca atatttattg agctcggatt
     8941 tagcgacata cttggagatc agcaatttga ttgctggaag cgactatgtg acttctgata
     9001 ttgttgtggc ggcagggaca gacgaaaaag agtataatag tgttatagca gcaaaaacag
     9061 cagatacatt gctttcgatg atcaatatca atgcagcttt cgtcattaca aagcggacag
     9121 acggcttgat tgggatcagt gccagaagta ccggcacgat caacgtccaa atcatcatgg
     9181 aatccttagg tggcggagga cacttcacaa acgcagctac tcaactggaa aatacgacag
     9241 ttgaggaaac aaaacagaag ctatttgctg caatcaaaga aaatatgaat caaatatatg
     9301 ataaggagta atccaaatga aagtaatttt tttacaagat gtaaaaggaa aaggtaaaaa
     9361 aggggacatc aaagaagttc cagcaggtta tgcacaaaat tatctaatca aaaataactt
     9421 ggcaaaagaa gcaacgaaaa gcagtatcag tcagttagca ggacaaaaga aagcacaaga
     9481 aaaacacgaa gctgaaatct tggaagaagc taaaaagtta aaagaattct tggaaaaaga
     9541 agatactgta gtcgaattga aggcgaaagc aggggaagac agccgtttgt ttggttcgat
     9601 cccttcaaaa caaattgctg aagggttaaa caaacaatac aaaatcaaat tagataaacg
     9661 aaaaatcgaa ttagaaaatc cgatccgttc actaggctac acaaacgtac ctgttaagct
     9721 tcatcatgag gtgacggcta cgatcaaagt tcatgtcgta caagaataac aaatcttgat
     9781 agcgatgttc agctggtaag tataaccaga aggaaaaaag acaaaccgaa atttatctga
     9841 gaaaacagct gcagcaatga aagcaaaagc tgcaaatcga tttcagattg atttcggttt
     9901 cttttttggg aacagcaaaa tagcataaag aaaacgagta taataaaaga gaactatttt
     9961 tttattacac tttcggtacg aatgatgact tttctctcat tttcttgtaa aatgaagcaa
    10021 ttgaagacaa ggagcgatac gtatgaatga agtgtggcag gatcgaatcc caccgcaaaa
    10081 tattgaagct gaacaagcgg ttttaggctc tgtttttctt gacgcagaaa cgatcattga
    10141 tgccatggag tacatagagc caaaagactt ttatcgtcgt ggccatcaaa ttatttttga
    10201 gacaatgatc gagttaaatg atcgtaatga agcgattgac gtggttactg tcaaagaccg
    10261 gctggaacaa gccaatttgt tagaagatgc cggtggttta agttatctct cagacttggc
    10321 attagcagtt ccgacagcag caaatatcgt ctactatgcg aaaatcgttg aagaaaaatc
    10381 attgctgcgt acattgatcc agacagcaac aggaatcgtt acgaaaggat tcgagcaagg
    10441 agaagaagtt caaacaattt tagatgatgc cgaacgcagt atcttggagg tttcggaaaa
    10501 aagaaatcga agcggctttt tatctattgc tgaagtactg aactccacga ttgaaaatat
    10561 cgatcgacta gctcaaaaca atgaagaaat cacaggtcta ccaactggct atcaaggtct
    10621 ggataaaatg acagcaggtt tccagcccga agaactaatc attcttgctg cacgtccagc
    10681 ggtaggtaaa acagcgtttg cattgaatat tgctcaaaat gtaggaacaa aaacagatcg
    10741 ttctgttgcg atatttagtt tggaaatggg ggcagaatct cttgtaaatc ggatgctttg
    10801 tgcagaaggt tcgattgagg ccagccattt acgtacaggt cagctttcgg aagaagaatg
    10861 gtcgaatctg atcgttgcga tggggagtct ttctcgagca aatattttta tcgatgatac
    10921 accagggatc aaaatctctg aaatccgtgc gaaatgtcgg aaactagcgc aagaaaaagg
    10981 caatctaggc ttgatattga tcgattattt gcagttgatt gaaggtaacg gtaaagagaa
    11041 ccgtcaacaa gaagtatcag atatctctcg ccaactgaaa aaactggcga aagagctgaa
    11101 agtacctgtc attgctcttt cacagttatc tcgtggagta gaacagcgac aagacaaacg
    11161 accagtgcta agtgatattc gtgaatccgg atcaattgaa caagatgcgg acatcgtagc
    11221 cttcttgtat cgagatgatt attatgaaag agaaggcgga gatgacgatg atggaaacga
    11281 gccaaaaaat gacaacgtca tcgaagtcat catcgagaag aaccgaagcg gtgcacgagg
    11341 aacagttgaa ctactgttta tcaaagaata caataagttt tcgtcgcttt cgccaagaga
    11401 agaattttaa agaagaggtt gtgagccttg agcaatcccg agagctctcg taagaagatg
    11461 caaggagtaa ccgcaggaac gactagagta cttaatttaa acagatctcc aaagggattg
    11521 tgacaaaagt cttgtcacag tccctttttc cgaataaacg gtgttcaatc agctgaccct
    11581 tggtattagc tcagcaaacg tcaaaatatg aggaactatt ttgacgtttg tttctctaat
    11641 actcaggtca aaacgctgat cttacgacct cttttcctta aacggtgttc tcctgccaac
    11701 tcctcggtca caagtcacaa tatcatcaaa catgagaagc agttttttga aattgctcct
    11761 tgtgactcag agttaaacgg caggctcaca acctctttct taaacggtgt tcaaaagctt
    11821 gcatctgcgg taatacgccc aatcaaacga aaaatatgag aagctatttt tgtttggctg
    11881 ttcttattac ttgatgcagg acagcttttt cacaacctct gattcacggt gttccaaaag
    11941 tagcttcttc gaaaaataag tcgaaatttc acaaaaattt gagaagcaat tttcgtacat
    12001 tccttcttat ttcttgaagc tgaccacttc tgttacaacc tcttttttaa taaccattct
    12061 aaaaagataa agttcgtttt ttaagcgttt ttataaaata aaaataaaaa tcattcgatt
    12121 ttatttgaaa tatgtatcta ttattgatac aatggttcag gattaaataa tatagaacga
    12181 ggtgttcaca tgtcatctgt agtagtagta ggtacgcaat ggggcgatga agggaaagga
    12241 aaaatcacag actttctcag tgaaaatgct gaagtgattg cacggtacca aggtggcgac
    12301 aatgcagggc acacaattaa gtttaacggt gtgacttata aactgcattt gattccttct
    12361 gggatttttt ataaagacaa gatcagtgtg atcggaaacg gcgttgtagt caatcctaaa
    12421 tcattggtta aagaattagc ttatttacat gaaaaaggtg tagaaacaag taatttgcgt
    12481 atttctgacc gcgcgcacgt aatcttgcct tatcatatca aattggacca attacaagaa
    12541 gatgctaagg gcgaaaataa gattggtaca acgattaaag gaatcggacc tgcctatatg
    12601 gataaagcag ctcgtgtagg tatccgtatt gcagacttat tagataaaga aatttttgaa
    12661 gaacgtcttc gtttgaatct agatgacaaa aaccgtcaat ttgtaaaaat gtttgattca
    12721 gaaccacttg attttgacga tatttttgaa gaatattatg aatacggaca acaaatcaaa
    12781 caatacgtca cagatacttc cgttatcttg aatgatgctt tggatgcagg caaacgcgtg
    12841 ttgtttgaag gcgcacaagg tgtaatgctg gatatcgacc agggaactta tccttttgtt
    12901 acttcttcta acccagtagc cggtggggta acaatcggaa gcggtgtagg gccttcaaaa
    12961 atcaataaag ttgtcggcgt ctgcaaagcc tatacgtcac gtgtcggtga tggaccattt
    13021 ccaactgaat tatttgatga aacaggagaa caaatccgcg aagtaggacg agaatacggt
    13081 acgacaactg gtcgtccgcg tcgtgtaggc tggtttgatt ctgtagttat gcgccattct
    13141 aaacgagttt caggtattac caacctatct ttaaactcga tcgatgtatt aagtggattg
    13201 gaaacagtaa aaatttgtac ggcttatgaa ttagatggcg aactaatcta ccactatcca
    13261 gcaagcttga aagagttgaa ccgctgcaaa ccaatctatg aagaattgcc aggctggtca
    13321 gaagacatta caggatgcaa aacacttgca gaactgccag aaaacgcacg taactatgta
    13381 cgtcgtatct cagagcttgt tggtgtacgt atctcaacat tttctgtcgg acctgataga
    13441 acacaaacaa acatcttaga aagcgtctgg gcgcaaatct aaggataaaa acgacgactg
    13501 aatcaggacg ttcttaccag agcgagtcga agtgtttatc tatacgataa taataacttg
    13561 aagaggtagt gaaagagacg tttagcctta ttgccgatag gctgtctctt gaacactgtt
    13621 tattcagaat aagggactgt gacaaaaacc gtgtcacagt ccctttttcc gaacaaacgg
    13681 tgttcaaaag cttgcatcta cggcactaag cccaatcaga tgaaaaatat ggagtgctat
    13741 ttttatctga ttgttcttac tgcttgatgc agagcagctt tttcacaacc tctccactaa
    13801 acggtgttct cctgccaact cctcggtcac aagtcacaat attcaacaaa catgagaagc
    13861 tgttttttga aattgttcct tgtgactcgg agctaaacgg caggctcaca acctcttggt
    13921 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
    13981 ctattttttc cgtttttttc tctaatactc aggtcaaaac gctgatttca caacctctga
    14041 ttcacggtgt tccagaagta gcttctacgg aaataagccg aaatttcaca aaaatttgag
    14101 aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctct
    14161 tattttgatt agaacttgcg aaaataaacc ggttaaagtg tgtctagcgc aagagatttg
    14221 ctataataga cgataagggt aaaatgtaac gagaaggggt tgaaaaatgt gtatcaaatt
    14281 gtaacagatt cttgttgtga tctgccatac catgtattag caaaagaaaa agttgatttt
    14341 ttatcaatga atctccaatt gaatgatcaa gagttagtag atgacctcgg aaagacattc
    14401 aattatgacc gctacatgaa tcagctgaaa aatggtgcga tgcctacgac atcacaaatc
    14461 aatgttggcc gatatttaga atttttccgt tcttatgcgg aaaaacagat tcctgtttta
    14521 tatgtggctt tttccagtgg attaagcggc tcttacagta gtgcgcttca agcggttgaa
    14581 ttgctgaagg aagaatatga agaccctgag atctatgttt ttgatacaaa agctgcaagt
    14641 attggggaag ggcttttagt attggaagct gtaagattga aaaaagaagg taaaagcatc
    14701 gaagaaaccc tagagtggct ggaaagtaat tatttgaaag tccattcatg ggtaacagta
    14761 gacgatctga aatatctaga acgaggcggt aggatttcca aagcaagtgc tactcttggc
    14821 agtctattga gtgtaaagcc tattattacg gtggataaag aaggaaggct gacgaatgtt
    14881 gacaaagttc gaggaagaaa caagtcgatc cagacaatcg tcaatgagac ggtaaaaaca
    14941 atcgttgcac cacttgatca aatcgtttat gtggcttacg ctggggatct agagagcgcg
    15001 gagaaggcaa aacggctttt ggaagagcag ataaagatga aagatgtgaa gctttatccc
    15061 ttaggaccaa cgatcgccag tcacacaggc tatggctgta tcgcgatttt ttcgatggga
    15121 atcagtcgat aaaagcagta actcattctg taaaaaagcc cagaaattga ttgtaacaaa
    15181 aacagcgact cttaaaagat agatgagcat cctatctctt agaggtcgct gtttttttat
    15241 tttttaagct aaataggcca ttatgcccat aactcgttca attgtttttg aatatctgca
    15301 ttttccaaaa attcatcata tgtcgtatca gctcgatcga tcacaccttt atccgaaacg
    15361 gcaatgatgc ggtttgccaa tgtttggata aattgatggt catgtgacgc aaataagatt
    15421 gagccagtaa atgccatcaa gccgtcattc aatgctgtga ttgattctaa gtctaaatgg
    15481 ttcgttggat catctaatac aagtacgtta gctttcgaaa gcatcatttt tgaaagcatg
    15541 caacggactt tctctccacc agataaaaca ttcactggtt tcaatacttc ttcaccagaa
    15601 aacaacattc ttcctaagaa gcttcttaag aaggtgttat catcttcttc tttacttgca
    15661 tattgacgca accagtctaa aatcgttaga tcagtatcga attctttcgt tgtatccttt
    15721 ggtaaataag aattacttgt tgtcacaccc caacgtactg ttccagtatc tggcgtaatt
    15781 tcacccatga taactttgaa tagcgttgtt gttgttatat cattttctgc gacgaaagcg
    15841 accttgtctt ctttgctcaa attaaaagat atatcatcta aaattttctt tccatcaatc
    15901 gttacactta cattttctac ctgcaataag tcgtttccaa tctcacgttc aggtttgaat
    15961 ccaacgaatg gataacgtct agaagaaggt tggatatcgt ctaaagtaat tttttcaagc
    16021 atttttttac gtgaagttgc ttgttttgat tttgatgcat tcgcactaaa gcgggcaata
    16081 aattcttgga gttctttgat ttgttcttct tttttggcat ttgcgtttgc ttgaagttta
    16141 gccgctagtt gactagattc caaccaaaaa tcataatttc ctacataaag tttgatttta
    16201 ccaaaatcta gatccgccat atgtgtacat actttgttca aaaagtgacg gtcatgggat
    16261 acaacgatca cagtattttc aaaattgatc aaaaattctt ccagccaatt gatcgattgg
    16321 atatccagac cgtttgttgg ttcgtctagc aaaagaacat caggtttacc aaatagtgtt
    16381 tgtgctaata acacttttac cttttgtcca ccagttaatt cactcatttg ctgatggtgc
    16441 aattcttcag ggatgttcag accttgcagt aaaacagctg cttctggttc agcttcccat
    16501 ccattaagct cagcaaattc tccttctaat tcagctgctc gaataccatc ttcgtcagtg
    16561 aaatcctctt tcatgtaaat cgcatctttt tctttcagca cttcatacag acgcttgtgt
    16621 cccatgatga ctgtttcaag tacagtatag tcttcatagt cgtaatggtt ctgtttcaat
    16681 acggccatcc gttcatctgg tcccatagta acggaacctg tggaaggctg cagatcccct
    16741 gataacactt taaggaacgt tgatttacct gcaccatttg caccaatcaa gccatagcag
    16801 tttccggggg taaatttaat attcacatca tcaaatagtt tgcggtctga aaagtgcaaa
    16861 ctcacatcat ttacagtaat caattttttt cctcactttt atcattatta gttttgacaa
    16921 gtcttataga attatatagg ggaaaggtaa aagattcaag gcaatcgaac attctttgtg
    16981 tttcacgtga aacagaatga aaaaaataaa aataaaagcg gagcgaaaat agctactata
    17041 ggatttatta gtttgcaaca gcataatttt tgacgttatt tataagaggg agtactataa
    17101 aactgtaaaa gaaatgaaag aaggattgaa aatggatgaa aaaagaacca tcgactgggg
    17161 ctcattgatt ttgggaattt tatttgtact tgtctcattg ttatcatttc aagaccctgt
    17221 cggaaattta gttgcaattg tagtagtctt tgcgattttt gcctttatta aagggatatt
    17281 tgaactgttt gtacggaaca gattaaaaga gctaacagga tataaaggaa agatgccctt
    17341 aataatcggg atcatcgata ttctagttgg cgtctttttc ttattcaata ttggtgcagg
    17401 agtcgcagca ttgccttttg tttttgcagt atggtttatt gctgattcag ttttagcgtt
    17461 atttactgct gacttggcaa gaggagtaag tgaaggatat tattggttta cgatcatcgt
    17521 caatatttta ggaattttat taggaatttt cttattgttt aatccaattt cttctgcgtt
    17581 gacattaagc tttttagttg gattctattt tatgttattt ggtatcacac atatcgtcta
    17641 tgcatttaga taagtaaaat caaacaaata aaaaagccgg cttccgatta cagttttact
    17701 gttgtaattg gaagccggct tttttatcat ggacaaatat gcacgaggtg atttttaggt
    17761 gtcgagacaa atttatgcaa aaatgtgaga agctatatac acaaatttta tcttaatact
    17821 cgaagcatag cgcagttgtc acaacctctt ttcatataat tcattcaata ctggcaaatc
    17881 atggacttca tatttttctt tgaatgcttg gatttcttct tcagaatatt ttcttggatc
    17941 taaatgcaga tcttctactg gcaatgggcg cttgttattg atcttcacat cgattactac
    18001 aggacgatct gattttgcag ctgcatcaaa ggcaaatcga agttgcgagt attctgtgac
    18061 tgtaaagcta tttgccccga gcgcttctcc aactttaccg aaatctgcat cttctaaaaa
    18121 tacaccaaat ttcttttgtt ccgtatcttc ttgttctgct tcgatgaaac caaaagaatc
    18181 attcgaaaag acaacattaa taattggaag ctgatatttg acttgtgtaa tgatatcttg
    18241 catgaccata gcaaacccgc catcaccact taaggtaaat acttgttttt ctgggtagct
    18301 tagttgtgcg gcaattcctc ctggcacacc attacccata gttgcgaacc atccagatgt
    18361 cgtatgtttt tgtttaccgt tcatattcaa taaacgaata gaatggatag tcgtgttacc
    18421 gacatctgtg acgaaaatcg catcgtcttc agcaatacga ttgatttctt tatagacagc
    18481 ctctggacga atcggttctt cctctcgatc ttcaaaagat ttcaaccaat ttacccattt
    18541 ttctttattt tcttgatttg ctaggtacca gctatcagca gggcgagcat cccctaactc
    18601 gacaagtctt tgcaaagctg tgtttgcatc accgaggatc gacaaagatg tttcatggcg
    18661 tcgtccaaat tttgacgcat cgatatcgat ttggataaat tcagcagttg gattaaaaaa
    18721 tgcacgtcca aatgggaagt cgctaccgac aaataagatc agatcggctt ctgccaatgc
    18781 ttcatttccg ggtttagtag cgacacgtcc agcaaaacca agaaagtttt cataatcatc
    18841 aggaataatt ccttttgcta gaacggaagc gacaacagga actgaaaaat gttctgagaa
    18901 ttctttgatt tgcggaaatc cgtttcgcgt tccttgacca acataaagaa ctggtttttt
    18961 tgctttttca agataaggca aagcagctag caaatctttt tcttctggta atatcacact
    19021 tgttttgtga gtgtgagcgg tggagaaagg ttgttcgtca atttcttcaa aaccaagatc
    19081 aacagggata gtcacgatag ccacgccttt atgctcatag gcggctttaa tggcttcatc
    19141 tactacatgc ggaagacttt gtggtgtcat gactgtacga ttgtatacgc taacatctgc
    19201 gaaaatagga ttttcattta gttcctggaa agagttatag ttcatggatg tagaagcaac
    19261 ttgtcctaag aacgctacta gaggcacatg gtccatctgt gcatcataca aaccattgat
    19321 caaatgtgtc gcaccaggtc ctgcagagcc gaagacagcg ccgactttcc ccgttaattt
    19381 tgcatctgca gcggctgcga gtgcgccgac ttcttcatga cgaacttgaa tgtagttgat
    19441 ttcggttttt tcttgatata gcgcatccat aattgaattg aaagatcctc cgggaatgcc
    19501 gtaaatatgg tcgatacccc agctttcaag aacttttacc attgcgattc ctgcattgat
    19561 ttttgacatg gtgtttcact cctttagcaa tgatgataca taaatttttt gtatctagag
    19621 tatagaactt tttatcgaaa atcccaacta attcgtcttt tcacaaaaat gtagtcatgt
    19681 ttttgggaat atgctagagt agtacataga tagattcaag agaggggaat ggcatggttg
    19741 aatggctatt gagacttgaa ccttggcagc aagcacttgc tgggacagct tttacttatt
    19801 ttatgacagc tcttggtgcc gggttggttt ttttctttaa agaaatcaaa aaagaagtgc
    19861 taaacttgat gttaggattt gcttctggtg tcatgattgc tgcaagtttt tggtctttgt
    19921 tagatccggc tattacaaaa gcagaagaaa atggagatat tgcttggcta gttgtaagta
    19981 ttgggtttgg tttaggcggt gtgtttttat atatggcaga taaaacactg ccccatatgc
    20041 attttggacc acaacatgaa gcagaaggat tgcctaccca tttaaaacgg acgatcttgc
    20101 ttgttttttc tattacattg cataacattc ctgaaggttt agccgtgggg gtagcattcg
    20161 gtgcagcagc aactgcggat aatccgacag ccgctatatt agccgctgtt tcggtggcgc
    20221 taggtattgg tatccaaaac ttcccagaag gagcagctgt atcaatcccg ttgcgacaag
    20281 aaggtctgag tcgtaaaaag gcctttgttt acggacaagc ttctggaatc gttgaaccaa
    20341 ttgctggagt gattggagcg ctactagtaa cgaaagtgga gctgctgctt ccttatgcac
    20401 ttgcttttgc cgcaggagca atgatttatg tcgtggtgga agaattgatc cctgaagcac
    20461 agcagacatt gtccagcaaa cgtcattttg cagtttttgg cgtcatgagt ggatttatca
    20521 ttatgatgat tttggatgtg gcattaggat aaataaaaaa aatatatatg atatatcaaa
    20581 tagggccgcg aacttatatc gcgaccctat ttttttagta agttatatca taatgaagtg
    20641 aatggtatca tacattaata atagtttttt ctatgtgtat ttcctttgat ccagcgaaca
    20701 agccagatga taagtgcgat tgggattaga actttgaccg caaaccataa aagcccgctt
    20761 aggacactga caaaaagact gatgacgaag ctagctgcaa ttagcactaa aagaaccatg
    20821 aagatattta agaacatatt tttttgacgc atacaaaacc ctcctcttgt tcattcttta
    20881 ctatagaaaa agtataacag ggattgttta aaaacacctc caactgaaga ctgattttta
    20941 cttcatctac ttaaatgatc aaacaattgc tttttctctg gtttatcatc taaaatagag
    21001 cggaaaagga ggagaatatc atgcctaaaa atcgtcttga agcatttaca gatgctgtaa
    21061 tcgcgattat catgactatt ttagttttag aacttcatga acctaacgga gacacgtggg
    21121 aggcgctcgc tgcattaggt taccgatttt ttatctatat tgtcagtttt atcattttag
    21181 cgatttattg gaacaatcat catcatttat ttcaagttgt acataagata aatggacggg
    21241 tcttatgggc gaacagtttc tttgtttttg cgctttcgct ttttccgttt gcaacttcat
    21301 ggatcagtga gtacatcaat agcctggctc cagaaatgac ttatggtttt gttaccttga
    21361 ccgctaatat tgcgtattat atcttggcaa aagaacttgt caaagcaaat aaaggccacg
    21421 agaaagcatt gaaggcgatg ttgaataatt acaagaaatc gtatctatcg attggtttga
    21481 atgtgttagg aattgtctta gggtatttag tagcgccagt agccgtgctg atcgcaaaca
    21541 tcgtgattct agctgcttgg atcatcccaa gtcgtaccat cgaatcacaa tatcggtaaa
    21601 atgatccgtg tttaaaattt aagagaaaaa tatagaaaaa ataaataaac gatcaaatat
    21661 ccgagtgata ctagtattca aagagggatg cagatggatc aaaaaagaat tcatgtagga
    21721 ctgattacta gttttgtcac gatgcttatt tatttggatc aaggtcattt gacttggaca
    21781 gtagttggtg tcgtaggatt tttgacatta ttcacagcaa taaaaaaata aacatcttta
    21841 tgtaatctga tgttctatct atttaagaaa agcatgttta cagcagtatg attctgttgt
    21901 aaacgtgttt ttatttgtgg aaaatttaat ttttagaaaa gtgtatcgaa aaatagatag
    21961 ttagtttttc cacagtggat aacttcgtaa gagataaaaa taaaaaatta gtcaaagaag
    22021 ggcgtataga tttcttttga taggagatat acaacagaaa tgaataaata ttaacgtgtt
    22081 ttcaaacagt ataaaacggt gtggtttttg gaataaaaaa ataacatatc tcattttaca
    22141 cttttgttat tttatactta aaaaataacg acacagaaaa taccgtgtct cagatttctc
    22201 tactacttct aaaaactact gtttttttcc tttgaaatgc cttcaaaaag taggatcagg
    22261 cagaatatac tggcagaatc aaggagaagg gagcctaaat aaggagaaaa accatagaaa
    22321 atataaagta attacttata aaacaagctt tttatagaaa aataatatac ctgagtaggg
    22381 tataaattaa gacggaattc ctttctagat tgagatattg actaggaaga atgttaagat
    22441 tttaagcctt taagctaaaa aaaaaccctt tttgttaaat aaggggaata tagcctgtca
    22501 gtttcaaact gcaagtcgtc ctgctgcaat aatatagtag attagagatg aaaatagatt
    22561 tttttaaacc aatgactgaa taaaaagacc gtcatagata cacattggaa tgtgtctatt
    22621 acggtctttt tttacgaacg attttattga agacggacag ttcgggtcaa aaataaaaaa
    22681 gctacgatca gaaataagaa taaaatgtat cccaaaataa tggctagacg gtctaactta
    22741 tttgtcgata gatgtttgtt tagaagttga taggcccata aacctatcag tcctcctgtt
    22801 gtattagtta tcaaatcagt gatgtccgct acgccaatac cgaataggaa ttgaatcaat
    22861 tctgccaaga agcttacaag gaaaataatc cctaattttt tagaaaaaga aagtctcttg
    22921 aaattgacat tcaaaagcac cccaaaagga aaaaagacaa tcaagttata agtggtctca
    22981 cgtaataccg tcgtattttc aaaagaaaaa gtagaaaaag ggacgagatt gacattgctg
    23041 taagaataag tgagaaattg tggaagatta aatgatagct tgaataaaat caaccataat
    23101 agaaagaata aataaatccc taataaaaat tttgatacat accgcataga catctcctcc
    23161 atttaaaaag tataccatcg aaaaccgctg aaaaatcata aaaaagtatg aagtttaagg
    23221 accaacgtag ttatcgtacg aaaattagaa gctttttttt gttgggaatc cggtttcttt
    23281 aacaaagcta agatagcgag ttgcttggaa aagttgctat cttttttctg tcaaaacgac
    23341 tcggaatgtt cgttttttgt tctaatttct ccaagttgtt tactttttga tactaagttt
    23401 atgaaaaatc aagaaatagt tgttatacta ggtaatgctg acaaacggga ttggcatttc
    23461 atcttggttc agcttttttt ccaattacta ggaaggaggg atagattgtt caagacttat
    23521 tttcgtttca ataaaataca agataatcct tttcgtatca tgaatctgat gctacttatc
    23581 tttattgtat tatggttagg ttacataaat catgattttt tgatttcaag tttttcatca
    23641 ttagggatct ttactttttt ctattatcaa aacataccga tgaaatattt gctgaaacgt
    23701 atgtttctga tcggttgcgg actgttggtt gctttcgttt tggggatctt atcgacttat
    23761 gttttatggc ttgaaccatt tgccgtagct ttggttgctt tttccagcag attcgtgttg
    23821 cgattattcc atatttctaa accaggtggc ttgttttttg cgatgctttc tgccatggga
    23881 acgagcatgc agctgccaat cgctcaatta ccgatcgtct cactttattt ttttatgggt
    23941 gtcgtttttg ccctgattgc ggcagtgatt acaaaattac tggattcacg gcctgagcag
    24001 acaattgaaa aagcaacttt aaaagaaaga tttcacgaag agcctttagt gatcattgat
    24061 tctgttttct acagcgctgc cttatttctt tccgtttatg tcagtcacgg cttgaatctg
    24121 cataatcctt attggttgac cttgtcatgt gcatcgattt tgctagcaga aaacttggat
    24181 gcgatgaagc atcgacaagt ccaatatctg attggatcga tgggtgggtt atgtgtgtcg
    24241 gcattcttgt catttgtgcc gtttacgcag ttgcagacca tttttctgat cacttttcta
    24301 tatggaatcg cacaatttct agttgctaga aattatgcag ttgctaatat ttttcttaat
    24361 ctgatggcgt tgatgctatc tacacttatt cgtggagcat acttgatttc tttgatcgaa
    24421 tatcgttttt tagggatcgt cataggaagt ttcatcggtt taggtgttgc gtgggtaatg
    24481 acggttggtt tgcaacatta tttaacggtt gttcgtcaaa aattagaata gaaggacaaa
    24541 tatatgtgcc aaaagaggtt atgacaagat ttttgtcaca acctctaatt caaggtgttc
    24601 aaaagccaga ttctcagcat taagtcaaat tcacacaaaa atgtggaaag gaactttcaa
    24661 aaaaatttta tatttattct atcaaaaaat tttgtttatg atggtaactg aataaacttt
    24721 cctgagatgg tcaatgcttt ccttgaaagt cttacagata ttctttcttc atagaaagtt
    24781 taagttttat agttagtgta caataaaagt atgtttatac catatggtgt ccggataatc
    24841 agtcatttaa acaatgaggt tcaaagatat gaaaagggga aatttgatga agaaaataag
    24901 caatgaaatc caaattctgg cagaaacttt aactcttccc aaagtcgtta tagagcaaat
    24961 gattgtacaa gcaaaaaaca tccttgatga ggtgtttgag caatatagtc atgaactaat
    25021 ggatcctaat aaagcaaagg cgactgcaaa gaaaatagac aaagtctatg aagaggaacc
    25081 ttttgcagca ttagctattt atctatatgc agcttctcaa agttggaaaa aaatttatca
    25141 accattgaag attccaagaa ctatctattt ggcaacaatg aatgctttta ctagatttat
    25201 tcatgaacat ttccaagtaa caggaagcta tcgctttgac cgtggttttt ggatttggca
    25261 ttacactagt ggcttgatct ttcggattgg tgaattggag tacgaaatga catattttcc
    25321 gaaagggtac acggatctaa gattagaagg aaagcagtgc ctttctcttc atattccttc
    25381 cgatgcagat ttatctcaga ataagataag agatagttac gaaagtgcca ttcgtttttt
    25441 tgaactttat tttcctgatt accattacca agtgatgtac acagatactt ggttactatc
    25501 acccaatctt actaaatggt tgaaaaaaga gtcaaaaatc tgtctttttg cagccgatta
    25561 tcgattatta tcagttgatg aacaggacga tagtggagtc ccttggatct ttggacgagt
    25621 agatgcccaa atacatgatt atccagaaag tacgagttta cagcgacaag caaaagagca
    25681 attgcttgca ggcgagcaca ttggctctgg cttaggtatt cttacttcat aagtttagta
    25741 cttcgaaata gtttaaaaga gcgtgagaca aaagtaaaga acacttttgt ctcacgctct
    25801 aaacacgtat aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa
    25861 aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa cgcttctgtc
    25921 ccagcctctt ttttagtgtt ctatcgaaca cacagtacgt tcaataggta aactagctat
    25981 aacaatagat attaaagtta acggacaaga gggccttttt taggtagcta aaacgtaata
    26041 atgaaaattt atttccagag ataggagtca atcagctagt ctgatcgact gttttaacaa
    26101 gttgactttc attttaactt ttgataccat ttaaaacaca aatcgtaatt gtttcgattt
    26161 gtgtttttta gcatgactac aatcaaagaa gaaagtgggt cgtccatatg gaaaaaacag
    26221 atatctcaag tgcataccgc cgcttgaaaa gcccaaatat caaaacacga aaacgtgcgc
    26281 tgaagatcat caaagatgtg aaacggaata gtgggaaaag gtaagaagaa ggaaacttaa
    26341 ttaaaaaaat agccgaaaaa cgtttttcgg ctattttcaa taatcggttt ttgtgtaagg
    26401 tttccgaaag aactgctgtt gattttccat ttttaacata tgataaattg gtagaatctt
    26461 tcttttcaaa ttgataccat tccctaattc cagttatata gttaactaca gaaccacaaa
    26521 gcacgtacgt ttataaaagt attatctgta gttatttttc aattcccttt caacctctta
    26581 ttttgaccag tagtattgat cactttacct tttaacacct ttgttcgttt ttgaaacatt
    26641 tttagtcaag aatggaaacg taactacata atcaaaggtt ttgcgaaata tttcatgaaa
    26701 tatttcatgt agaagcgagc aatacggttg caaataacca ttatcgttta ataagcagtt
    26761 acgaaagctc ccaatatctc taacgttcag ctcactccat ttcttttctt caaaaaaatc
    26821 agtgattcgt atctctttct atttgtcgaa aaagttaact ttaaaaagta acgccgaaaa
    26881 aatatgcaaa aaaataaaaa agcagttact attgctagaa taacatcaag gaataataag
    26941 ttttggtttt cagataatag tcgataagtt gtgtattgga attttagata cagtaataaa
    27001 gtatttttca tgaaattttg tgagaatcac tattacaagt atctttaata aaattaatgg
    27061 ttaaggagga acttaaagta gaatgtaaat gaatagttgt agtcagtcga ttgtgatact
    27121 agactcaaag ttgcaggaat aagtttttca atttgtgatt ggaagataaa aaatgaaaaa
    27181 gaagggcttt tttttatact ttgttgattg tagtgtttat tttccctctg tttagccaag
    27241 taacattttc tatgactcaa ccagtttcaa gtgaggaaca tgataacctt tttcgtgcta
    27301 cagaagagtt taagaaatat caggaaaata ttgttccgga gactccatta gctagtgagc
    27361 caattactga tattgatgga aaccaaatag gatggtatat acagtacgaa gttatggtgg
    27421 taaataatga ctcaatagta aaaaatacag taggcttaga gctaacaccg attgaaaagg
    27481 tgagttcatt gttaacgtat atttatactg atgaaagtga cgagactgag atcattttaa
    27541 cggattactc aaaaattgct actgataaaa agtatatatt gtggatgtag acactagtga
    27601 aatactttat gatatagaaa ttgatgaaaa tttactagat gtagcacaag aaatcgatca
    27661 tcaaaagcag gaagctattc gatatgctgg tagaaaaggg aaaagaaaat atttattacg
    27721 tgaatgtagc aaaagttaga gaagatgaaa atgagtggaa agaattcaag agtcgctact
    27781 caattaactc gactccgaca tttacagttt acagagaagg atcgatagaa aaaactgttt
    27841 tttggactaa agaaagtgga atgtctttag ctgaagtaga agagtttttg gattatgtta
    27901 gtatgcaaca gtaagggagt tcttattatg aaaaattttg acacatatta tgttattgtt
    27961 ttttcgatat ctttgctgtt aatattacta actttaatgc agtataaaga gtatccagag
    28021 tattttattt taacttttgt atatttagta agtagctaca cacttctata taaattcaga
    28081 agttcttcta gagtttctaa agtttgtttc ttaatt
//
LOCUS       DAHUVV010000024        28034 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.024, whole genome shotgun sequence.
ACCESSION   DAHUVV010000024 DAHUVV010000000
VERSION     DAHUVV010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.024"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(573..1517)
                     /locus_tag="NK767_001105"
     CDS             complement(573..1517)
                     /locus_tag="NK767_001105"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCH6694113.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1813..2757)
                     /locus_tag="NK767_001106"
     CDS             complement(1813..2757)
                     /locus_tag="NK767_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6694114.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2978..4192
                     /locus_tag="NK767_001107"
     CDS             2978..4192
                     /locus_tag="NK767_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6694115.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4214..6229
                     /locus_tag="NK767_001108"
     CDS             4214..6229
                     /locus_tag="NK767_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HCH6694116.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6312..7685)
                     /locus_tag="NK767_001109"
     CDS             complement(6312..7685)
                     /locus_tag="NK767_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCH6694117.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7711..7995)
                     /locus_tag="NK767_001110"
     CDS             complement(7711..7995)
                     /locus_tag="NK767_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6694118.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7997..10012)
                     /locus_tag="NK767_001111"
     CDS             complement(7997..10012)
                     /locus_tag="NK767_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCH6694119.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10191..10496)
                     /locus_tag="NK767_001112"
     CDS             complement(10191..10496)
                     /locus_tag="NK767_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HCH6694120.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10585..11616)
                     /locus_tag="NK767_001113"
     CDS             complement(10585..11616)
                     /locus_tag="NK767_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCH6694121.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11823..12698
                     /locus_tag="NK767_001114"
     CDS             11823..12698
                     /locus_tag="NK767_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCH6694122.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12850..13692)
                     /locus_tag="NK767_001115"
     CDS             complement(12850..13692)
                     /locus_tag="NK767_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCH6694123.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13784..15661)
                     /locus_tag="NK767_001116"
     CDS             complement(13784..15661)
                     /locus_tag="NK767_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCH6694124.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15658..17079)
                     /locus_tag="NK767_001117"
     CDS             complement(15658..17079)
                     /locus_tag="NK767_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCH6694125.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(17082..20471)
                     /locus_tag="NK767_001118"
     CDS             complement(17082..20471)
                     /locus_tag="NK767_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCH6694126.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20728..22119
                     /locus_tag="NK767_001119"
     CDS             20728..22119
                     /locus_tag="NK767_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6694127.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22192..22839)
                     /locus_tag="NK767_001120"
     CDS             complement(22192..22839)
                     /locus_tag="NK767_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCH6694128.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22847..23533)
                     /locus_tag="NK767_001121"
     CDS             complement(22847..23533)
                     /locus_tag="NK767_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6694129.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23539..24612)
                     /locus_tag="NK767_001122"
     CDS             complement(23539..24612)
                     /locus_tag="NK767_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6694130.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24834..25508
                     /locus_tag="NK767_001123"
     CDS             24834..25508
                     /locus_tag="NK767_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6694131.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25513..26853
                     /locus_tag="NK767_001124"
     CDS             25513..26853
                     /locus_tag="NK767_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCH6694132.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(27002..27988)
                     /locus_tag="NK767_001125"
     CDS             complement(27002..27988)
                     /locus_tag="NK767_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HCH6694133.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8197 a         5273 c         5078 g         9486 t
ORIGIN      
        1 aaaaatatgg aaaatatgga ttaattttga aataattgat ttaaaaggaa tacaaaagag
       61 tgaatagtag gaaatagtgg tttagaacta gtgggctgat tttttatata ttttagttta
      121 cataatatat attatacaaa gtagatttaa aaaatgataa acctctctcg aacggttttt
      181 tatcaaaaat cgtcattttt ccatcaaaat ctgcttatgc aaaattataa aaaaattttg
      241 cagtttttct tacttttttt gtcgtttaat tgaatgataa aaaccagttt ctttattcag
      301 ttaatcaaat atgaacgaat tagatatgaa tcattgtatt tgccgttgcg gaaggatttt
      361 ttaattaacc acagataaac attgataacg attcgcttat tgtaatcaac agattaaatt
      421 tcatatgttc atatgtacgg atacattttc ggaccgatta attttaacca ttctgtttat
      481 ttgcttacct attagatatc aataaaaaac tgcttaacag ctatttcagc tgttaagcag
      541 ttttaggatt tttagttcat attcattgtt tcttataata cagtgttcaa tacttctctt
      601 aaagcacttg ttgatttgct gaacattgct ttttcttctt cgttgattga taattcaaca
      661 acttcacgaa caccattttc gtcaacgatt gatggcacac ctgtaaagat atctttttcg
      721 tcgtattcac cagttaaata agcagataca ggcaatacag cttgttcgtt attcaagata
      781 gcttttacga tacgtgcagt actcatacca ataccatagt aagttgcttt tttacgatca
      841 atgatctcat aagcagcatt acggacttta tcagcaatca catctaattc atcttgggca
      901 atacgatggt ctttttctac aatttcaaac actggtttac caccgacagt tgtatgtgac
      961 catgcagcaa cttctgaatc accatgttca cctaagatat agccgtggac gctgcgtggg
     1021 tcaactttta atttcaatgc gatttcttta cggaaacgag ttgtatccaa tgttgttcca
     1081 gtaccgataa cacgtgatgt tggcagacca gattcgttcc aagcgatata tgtcaaaata
     1141 tctactgggt ttgaagcaac aacgataata ccgtcgaatc ctgatcccat gatttctttt
     1201 acgatttggc gcatgattga cgcatttgtt ttaactaaat ctaaacgaga ttgaccaggt
     1261 ttttggttga taccagctgt gataacaacg atatcagcat ctttacattc ttcatagcca
     1321 cctgaccata cagccacatt ttcatcgccc caagccatac catctaaaag gtccaatgct
     1381 tccccttctg ctttttcttg attcacatcg atcaatacta attcattaga gattccttgg
     1441 ttgatcattg cataagcgat acttgttcca acaaaacctg taccgacgat tactacttta
     1501 cgacttgttt ttttcatgaa taaacagctc cttttcataa ctatttgtta ctttcatgaa
     1561 aagtatagtc ttgtttattc attttatcaa catgttaatt ataatagttg ttattataat
     1621 ttttgatgga aacgaattcc tgaatacgca taacagataa atgacaaatg tttacaccta
     1681 tatcccctga taatacaacc aaaagtttag aactgaaaat aaatccaaaa aaattcgctg
     1741 caacgatata ataagacagc aatcgcacct aaaacaattt tccttcatat gctttagtga
     1801 aagattttct tcttaatgtt tatttctcca ttcattggga gtacatgttg aatacttctt
     1861 gaataatttg aataaagaag tcgtttgttt atatccgata cgattagcga tttcttccaa
     1921 cgaaaaactc gtttcttgaa tcagttccaa cgcaaagtcg aattttttct ttagaaccag
     1981 ttcttgaaag gaagcatcaa aatacatttt caatttatta ctaagataga ccggattgta
     2041 gccaaaatga gctgctgttt cttttaacgt acaagaagta aaatgcttat cgatataatc
     2101 taatactcta agcatctcat tttctgcgca tccatctgct gatgaaaagc tttgttgttc
     2161 taataaatga gtcaattcga tcagtaatag tgatgtatag agatttaacg aacggatctt
     2221 atgactgtat gtatcaaaat aaatcaacag catattttca atcaaatgcg tcaaacgact
     2281 tgtttttccg ccgcgaaaat gaatgaattg tgtatgttcc cctttttctt ttgatgcatt
     2341 tatcaaaaaa gaagaaagta gattatttgt gcttgccaaa tgattcaata tctctgtaga
     2401 taaggtttct tcttttaata agatgttgat taaaatatcc tcttgccctg tgtattcgat
     2461 tctttgagta gcttctttat caatgatcac cacgtcactt tcttccatct ctatcctttc
     2521 tccattaata atttgtaaac atgtaccgct atacatataa ttgatttcaa caaaatcatg
     2581 tgtatgctcg ggaacttccg catatcgacg gtgtttactg atatgaatat ttccctgttg
     2641 aaatagatcc cctcggtgga tttttggcgg tgtctgaaga tggtaagcta agtttaaatc
     2701 attgatattc attccttgtt cacgctgcag agtttcgatt gattcttcgt tacgcaaaaa
     2761 aaatgtctaa ttcagctagt tccaattgac tccctccttt ttgtaagcgg tttctttcgc
     2821 ttattagttc acgctgtact gctattatat gcaaagctat ttttggtttc ataaagactt
     2881 tctatggttg ggacatgaac ttcctgtgat tccgctattg agagaataac cacttcatac
     2941 tatatttttg ataagcgctt acaaaaatga aaggaacatg cttatgaata aaaagatgat
     3001 gaaattttct ctgctgtcag tctctcttat tttgacttct gctggtgcag tcacgtctgc
     3061 aattcccagt atgtcagcta cttatgccac gcgttcttta tctgctgttg aattattaac
     3121 gactgctccc tcttttatgg tcactatttt tgttttgatt agtagtttta ttgctaggaa
     3181 aatcggacaa aaacaaactg tgatcatcgg attagtgatt gcatcgattt gtggtatcct
     3241 ccccctcttc tcacaaagtt acagtattgt cttagcttca agagctggac taggtattgg
     3301 ctttggattg atcaattctt tggcagtcag catgattgct gccttttatc acggagatga
     3361 acgagcctct atgattggtt ttcaaagtgc atttcaagga tttggtgcag ctttaatgac
     3421 atttgttgca ggacagctag tacagttcgg ttggcaatat acctttttag tctacgccat
     3481 cagcttacct attcttgtct tgtttgtttt gtttgttcct acacctgaaa caactgaact
     3541 tcaatcgcat ggcacagccc caacaagaca agtaatcaaa acgaatccta aaatgattag
     3601 ttatggtatt tttctcttgc tggtcatcgt ggtatataat gctgtatcga ttaaattagc
     3661 cacagtcctg acgacttatt ctctaggaaa cgaaactaat gcagcgaata tcttaacaat
     3721 catgcaattg gcaagtatgg ctgccggagt ttgttttggc tggcttcaga caaaagcaca
     3781 tcgctatatg cttacacttt ctttactact gatgggaatc ggctttgtat tgattgccac
     3841 tgtgataaac ctgtatgtga ttgggtttgg tgcgatccta acaggaattt ctttttcgtt
     3901 attcattcct tacttattca accaagtaag tatccaagcg cccaagtacg cggagagttt
     3961 caatacttca atcttattgg ttggcgctaa tttaggtact ttcattgcac catatggttt
     4021 gctctttctc tcttttgctt ctcttgggag taaaacactt gctgttttcg tcaatggcag
     4081 tatcttgctg ttgattagcg cgcttattag ccttctagta gtacacagga cgaatagaaa
     4141 gacagtgata agagtagatg aagaaataaa agaacaattg acaacaaaat aaatttgatg
     4201 gaggtcgtgt aaaatgacaa atgaaaaaca taaagataga tggctttggt atcctggaga
     4261 ctttgagatc cgccatggac tgctacaaaa tttccaacgg gaagaacgag gttttgactg
     4321 gccagcgtac tggtacatgg atgattgcca tcggaacgtc aagtttaaga gatattactt
     4381 tcttgatcaa ccttccatgt ttaaagtgac gatccaagga gttgggtatg tagaaattaa
     4441 tggacaaaaa catccatgtg gaaaatggct gacttgtcca gctggtaaag caaaaattcg
     4501 tatctttgta ggacatacat cagggctacc agcgatgttt atcgagggag acgaagtaaa
     4561 atcagatatt ggctggacag caagtaattt tattgaagaa tatccagctg gctggagtcc
     4621 gctttatgtc gatatcgcaa aagatccaaa tcaaatctat tatcaaaaag aatgcgtcca
     4681 tccagtcact gaaacggaga ttggcggagg aatcttattc gattttgggc gagcagtgaa
     4741 tggctgtgta gaactaatat ctctggatga ccatatcgaa tctatcactc tatgttatgg
     4801 agagtccgat gtagaagcta tggatatcga ttattgctac tataagcaaa taggaatgac
     4861 aagaaaaatg aaagctcgta aacgcgcttt tcgctatttg tttataccag aggtttctac
     4921 tggaatggtt caacttcaag tttttcatga atttttatct ctttcgaaac aagctgcttt
     4981 ttcaagcgat tcttctttac tgaatcagat ctggtcagta tcagaagaag ccttcaagtt
     5041 atgtagtgat ttgtttttta tcgacggtat caaacgagat cgctggattt ggtctggaga
     5101 tgcgtatcaa tcattcatga tcaatcaata tctttttttt gacgaggaaa tcaataaacg
     5161 aacgatatta gcattaagag gacaaaatga aatcaagcag catatgaata ctatcgttga
     5221 ctattcgatc ttgtggttga ttggtatcga aaaccactat atgatgacga aagactatga
     5281 atttttacgc atcgtctacc ctaagatgca atcattaatg agatacttga tggagcaaac
     5341 gaatgaatta gggtttattt atgggagagc aaaagattgg atatttattg attggtctga
     5401 aatggataaa gaaggaacgc ttgctgcaga acaagtctta ttactgaaag cctatcattc
     5461 catgatcact tgcggagata tcttagctga aacatatgga gagaacgaaa attatcatac
     5521 tcatctctat caagaaaaat atcaactatt gaaacaaaac ctatttgact acttttggga
     5581 tgaagaactt ggcgctttta tcgattgcta tgaatctggg agaagaaatg tgacgcgtca
     5641 tgcgaatatt ttcgctgttt tattcgattt tgctaacaaa gaccaacagc aatcgatctt
     5701 aaagaatgtc ttattgaatg acactatcac acagattact actccatatt tcaaattcta
     5761 tgaacaagat gctttatgca aactcggaca gacgaatatc gtctaccaaa cgattcttga
     5821 ttattgggga gggatgttat ctcatgatgc ggtaactttc tgggaagaat acgaccctac
     5881 tcaaacagga aatgaaaagt acagtatgta tggagatcct tatggaaaaa gcttatgcca
     5941 tgcatgggga gcaagcccaa tctatctttt aggacgctat tttatcggtt tgtatccaac
     6001 aaagccaggt tatgaagaat ttatgataca acctaatcta gattcatttg aaacactgaa
     6061 ttgtacgcta cctatcaaag atggaaagat ttcgcttgaa aaaacaaaag ataagatcgt
     6121 gattacttct gatagaacag gcggaacagt gaaaatgaat ggtaaaagtt atcctcttct
     6181 agcaaaacag ccacttgtct taacaagcta ttcaaaaata aatgcataaa acaaaaaact
     6241 gcttgcgaca tatcagaaga ctttaaatct tcttctgata ttgtccaagc aatttttatt
     6301 ttgaagtttg cttaattctg ttctttagaa acaatgtttg ttctttttcc ggcaaaaaga
     6361 aagctagata atacgacaag taaaaagaaa gcgatgattc caactgtcaa ggctatttga
     6421 ccgtattctc ctagtttccc aagtacaagt cctggtacta gataatcagt atcggcaaat
     6481 gtagaagaag cgattttaag atctcctaat actggaagga taaataatgg cagccaacta
     6541 atcaataagc tattgaaagc agaacctaat accgcccctc ttctaccgcc agtcgagttg
     6601 ccgaagacac cagaagcagc tccgcagaag aaatgtccta ctaccccagg aatgataact
     6661 gtagtttgca agcctatcat agcaaacatg gatagtgttc ctacaaagaa gctaacaaaa
     6721 aagccaatta atacagcatt aggtgcatat ggaaaaacga taggaacatc taatgctggt
     6781 ttagaatcgg gtacgagttt tttagcaatc ccttggaatg ctggaacgat ttctgccaac
     6841 accattcgaa ccccttgaag gacgataaca aacccagctg tgaatgttcc agcttggatc
     6901 agtgcaaaga ttacagcgtt gtctccattg cttagttctc tttcaacaaa tccaggacca
     6961 gctaacaaag caagtgcgac atatacgatc gacatcaaga gcatgatcga tacagtagaa
     7021 tctcgcaaaa agctgaaact tttaggaatc tttatgtcct ctgtagaacg gtctttgttt
     7081 ccaaaaaact ttccgatcca tccactagaa gcataaccga tatttccggt atgccctaac
     7141 gcaacttgat tgtttccagt gatgttcttc ataaacggtt gacaaagagc aggcgtaata
     7201 gttaatagtg ttccttcgaa gaccccgcct aataagatca tccacaaatt tgcagtaaac
     7261 cccgcgctta ctaaaatgac tgctgttaga caagaaacat aaagcattgc ttgacctgta
     7321 agaaaaatga atttatatct tgtgaatcta gcaagtaaaa tattcacgat catccctact
     7381 agcatgatca aagcagtagg tgtaccgtat tgttgcaaag caatcgccac tactgcttca
     7441 ttgctaggaa ctactccttg agtatgcaga gctgtttgga acatcgaagc aaaaggggtt
     7501 agtgaattac tgattaaacc agcaccgccg atcagtacta ggaatccgac aaatgttttg
     7561 atagttcctt ggatcacttc tgaaaatttc ttcttctgaa gaacgagacc aagcagtgca
     7621 atcaaagaaa cgaggatgga gggctgactg acgattgaca caaacaaatt caacaattta
     7681 tccattgata gttcctccct tgaatcattt ttattctttt gacaagatct tcgttacttt
     7741 ttcttttact tcttcgctgt caatgatcga ttgcaaaggt acgagctttt catcaggtat
     7801 agaagttagc gtatctttca aagaatgctc aatgaagaaa taatcagcca tatcgctaga
     7861 tacacttcca acatccgtat gacttacttc aaactcgtcc acatctgctc caagatctgc
     7921 aagtgcttgt ttgatgttca tctctaccat aaaacttgtt cctaatccac tttgacatac
     7981 tgcaaaaatt ttcatatcat tcgtctcctt ttcttatgat cgctaatatt tcttcttttg
     8041 ttgatgcgtg taataaatct tgaagatttt gtttattaga aagtagcttt gttaaatttg
     8101 ccaaagcccg aagatgtgcc tcattatcga tagcagctaa acagatgaac aaactaatgg
     8161 gatgggctgg attatcaagc agatccacag gttgttgaag tttcagcaaa gacatcccta
     8221 cttcgtttac gccatcttct ggacgagcat gaggtaatgc gatgtgatcg ccaatatgga
     8281 taaacgcacc atactgctcc acgcgctcga tcatagcttt gatataactt tcttctattt
     8341 tattttctga taatagcggc tgtgcggcaa atgtgattgc ttcttgccag ttttttggtt
     8401 gatccgataa ttggatcatt tcttctgtta ataattcaga tagcatagga cgttgatcct
     8461 ccttcttttc taaaagtttg ttcattttac gatttaatac ttgatagagc ttttcttcgt
     8521 ctacgccttc ttttaattcg atataaggca gtagcgccga caacaattct tgggcagaag
     8581 gcattgaaaa tcctggtatc agccaatctc tttgtacttg attgatcagc tgattttttt
     8641 ccaagtcagt catgattgga tgcagaacat agagagactt tttcttatca gtaagtgtga
     8701 gaggtacagt agaaaaaata atatcgtaag aagtttcgtc aacctcgtgt aagcggtcag
     8761 ctgaattagt ctctgtaaaa aagatcatcg ggaataattg ctgaagttca ctttttaaga
     8821 tcaatgaaga gctaatgcca cttggacaga cgatcacggc tcttattttt ctatttcttt
     8881 cttctgcgtc cgcttttctg atctctccgc caaatagtat agtgaaaaaa ccaatctctt
     8941 cttcagggat gctcttccct attcgtcttt ctaacggaag taatgctttt cttgtcattt
     9001 cgtagatgga agcatattga tgcttaattt gatcgatcat cacattaggt aaataaaaag
     9061 aataggaaat acgaaaatag gcagggacca agtgataaaa gacattcatc aataattcac
     9121 gaaacgaagc aaattctacg gccatcagga attccatgcg atggatgact tctgaagaac
     9181 attccagcaa aaaatcaagt gcaggctcct ggacatctcc ttcaacgatc gatgctaagc
     9241 aaagagagaa aaatataatc tccgaatttt gatgttcgga atagccaagg ccagtcaata
     9301 cttgtcgact aatgtcagtg actgcaagag aagccaacag ttcagcatct tttaccagtt
     9361 ccatggatgt ttttccaatt cgaatggttg tcaaactcaa ataataagct aattcatcca
     9421 ttcgatttgg tgcgaatcga agatctgata gtgaaatcgt tttatctagc aaatctctaa
     9481 ctttggcgta ataatcaatg gaaagggttg cggttaaata cgtgagccca aatcttccag
     9541 caagtgactg catcaaacga gcagtgcgat tttttgcttc tcttcttaat attgcttctg
     9601 accctagcag cctaaaacca gttttcctag tatacgacag ctgacaatct tccacttcta
     9661 attcatttct tatctttttg atatctgaca ggactgtccc tttagaaacc tgaagaaaat
     9721 cttgaaaatg aaaaacagaa acagacgttg cagtcgaata aaccatcaaa taaagaagat
     9781 ctcttcgctc ttgttcggta tacacagctt caaatcgagt aatatccaaa aatttttctg
     9841 cccagtcgat tgctcggaat ttctttatac ggatacgggt atcatccaca tattcggcaa
     9901 aaggagttaa ttgtttgatc gcctcgcttg tatttacttc gatgatctct gctgcttttt
     9961 ctagactgat ttctcttggt gttttcagga gataactaat tttgtagttc acagcgctgt
    10021 tcacctccta cttatattct agtatggtag cgcttttaaa tcgaggggtg tttggtacaa
    10081 cttacagttg gatttttttt acgactgtac cgaacgattt ctatcgaatg gatacagaaa
    10141 aaaagcaggt gaccaaatgt ctcctgcttt tatgaattga tttcgtccga tcaatgatga
    10201 tcatgtttat tttttgaggt cagttcttca aatttttctt tgacatcttc aaccgctttt
    10261 tcgatatctt tttcaaagtg attgggctca ttaggctttg tttcctcttc tacagaattt
    10321 gaaccagata aagctgtttc tttattttct gtttcaccag agtttgtgcc agctaatgat
    10381 tcttctttgt cttctgtttc accagaatta gttcctgcta aagccacttc ttcttctgtt
    10441 ttaggggcca ttgaacctct taatcgtttg tgtttttcac cgtcataatt tgtcatgttg
    10501 gttcctccta gtagttctat actttttcat cgtacaataa gacagtttat gcgtcaaaaa
    10561 acaaacccga ttcttacaaa tatgtcatat ttttccttct gattcttgaa tgcttttgat
    10621 tccttcttcc aaaattttca tttgtaagat tgtttcttca tctttgatga ctttttctgc
    10681 gccattaact aatgtctcgt aaatcccttc gtagacacgg ctgtaatcac caacctcgga
    10741 aattactttt tcttcatgat aactcccttg ttcatctata taggtcaata caccataatg
    10801 ttcgggcaaa tcgataccaa agtcttcatg atcaggcatg taaaacattt tcagatgttc
    10861 ttcttgtcga tcttttgttt gtttcacgaa catccctttt tttccgtaaa cgacgaaact
    10921 tggacgttct ttgatgcgga aataactgga ttttatcgaa attttcatat ttccatagaa
    10981 aaaatccaga tcgaagtaat cattcattcg tcctttccct aaaagttgac gtacatcata
    11041 gctgatatca tctggttcgc cgaaataaga aagaacttga tctactgtat gacaagcatg
    11101 tccatataaa tagctgttcg cagtagaaaa ttcatgcgta ttttctggta cttccggtct
    11161 gaaataatca taatgcattt ctacttctaa aatatcccct aattttccgc tttcgattac
    11221 tttttggact gtcaagaagt cagaatcgaa ccgtctgttt tggtaacatt ggataaatag
    11281 acctttttct tttgctaatg caaataactc ttctgcttcc tttgacgttt ctgcaaaagg
    11341 tttctctact agtacattct taccatgttc gagtgccatt tttccatatt cataatgaaa
    11401 ttgactaggt gtagtgatga caactaattg gatctcagga tcatcccaaa gatcagacag
    11461 ctgatcagta tattcgatgt cttcgaatgt tttccacact ggttcagatg gggaaaaaat
    11521 ttttttgata ttgattttgt tcgtaagttt tcttgaaaaa ggcaaatgat agcgatttgt
    11581 actctttcca ttgccaagat aagcgattgt aagcatgagt ggtgcctcct tttattgatg
    11641 ttttcagtat atcctagaaa agcgcagaaa gtatttataa tagctattct ggtaaaaaag
    11701 ttcagaaaaa cacttgttat cattcggttt ggtgaaatta atgttcaaag taaggataaa
    11761 aatcaaaaag atagtagcgg aaccaagtgt ttatcagtaa gatagaattt ggaggtcaga
    11821 tcatgcttat tgaagaaaaa ctaacaaaac aagagttgtt taccacgaca gagaaacgga
    11881 ttgtcgatta catccgtagg aatatcgaag ctgcagtcta catgacgatt gaagaattag
    11941 caaaagctac gtatacttcc cattcagcta tcattcgttt atgcaaaaaa atgggattca
    12001 gcggatttaa agaattccgt ttcgagctgg ggcgggaagt tcatcaattg attgctcagt
    12061 tcaatcaaac gattgatcct aattttcctt tttcaagtga agatagcact cagcaaattg
    12121 caaagaaaat ggcggattta tcgatccagt caataaaaaa agcacaattg cagattgaac
    12181 atgaagattt agatcaaatc ggtaaagtat tgaacaaggc acagcgaatc tttctttttg
    12241 caaaaggtga ttctcaaatc acggcaagga aattccagaa taaaatggtc aagttgaata
    12301 agtttttgat catggctgaa gaatacagtg attcttcttg gaatgcagcc aatttgacta
    12361 gcgaggattg cgccattttt attagctaca gcggtcgcat ccatcattat gaacgaataa
    12421 tgacctattt gaagcatgta gaggccccta ccttgctgat taccggaaat cagcattcag
    12481 aaatggccaa acaggctgat atgtgtcttg ttatatcgca ggaggaatat gattttgcta
    12541 aagtagcgac tttctcctct caaattgcct tcgattacgt attaaatacg ttattctcag
    12601 tgatatattc tcaaaatttt gaagagaata tccgcaactt gaaaaacaaa caagaattga
    12661 tgcaaaaagg ccttcttaaa gaatatcgga aaacttaaga aaagatcctc atttagtaat
    12721 aagaaagata gtgtttgata ggctctcttt gatatcatca taaaaacagg ctgaagtttt
    12781 ctttttcaaa cctcagcctg tttgtttacg gaacgattcg aatagatata ttatagaaat
    12841 gaggaatagc taacttctgt ttcttatttt gaacgattga cgaaaaagat atataagaag
    12901 gaggaaaatc agtatacatg cagctaacaa tccatacagg aaaaatcgtt tgttctcgga
    12961 tactttctgt acttcttttt gaacttcttt tgcatgataa ggaacccggt gccctctgac
    13021 aagtaagcga tgactgttga ccatataagg tgtacaagtt aggagtgtca cgaaatcctg
    13081 ccctttttcg attctcagat cttttgtatc agtcggttct accgtcttga tctgatcgac
    13141 ttgataagcg agtgtccgtc cattgatttc gatatagaaa gaatcaccta aagtcagttt
    13201 cggtaagtct gtgaacaact ttgcttgagg cagtcctcga tggctggaaa taacggcatg
    13261 tgtattttct ccccccgtag gataagacgt tccttccaat aatgaggcac ctttttctag
    13321 cagcacagta ttcgtttcat caaaaattgg caaatggaca ttgatctcag ggatcgtcaa
    13381 aataccgatc gtatgtttct caaaataggt tctatccttt ttcgcaggtt cttttttctt
    13441 agtaaaggga tctgcacctg gatttccacc ttcttttgct aattgctggt ttttctttgt
    13501 catgttttct tggattttag ccatgacttc ttcattttct ttgaccgctt gttgctgata
    13561 atgagagatg atctgctgat ccagataatt attcagcgcg tcgctaacaa atggataagc
    13621 tagtatccct attcctgaga gaagcaagaa aagcatcagc agatctagaa gtttcctttt
    13681 ctttggtttc atttgtgttc tccttgtgat gactgtttga taataaaaga aaaaggagca
    13741 tttgcgtaat ccgctgctcc tttttccaat tcttgcaaaa agactaaatc tgactgtgct
    13801 tgcgtctagc aaagtacagt ccagcaatca gtagaaggac tactcctgca ccgatataca
    13861 catagattcc cttaccgcct gttgaaggaa gtgtaccttt gtgtttattt ggtatttttt
    13921 caggagaaat caactgtcct gctgttacat aagattgttc atcgatagta aatgctacac
    13981 ggtttgttaa tggaacatat ttttctggcg ctttcgtttc ttccagatag tacgtgccat
    14041 attttagacc tgtcacatcg attaaaccgt tacttgtggt tgtaaaaacc gttgctgatt
    14101 ctttcgccga tacccagctg acggcttttg tggatgggtc gatcgataaa tatttcgctg
    14161 tgtcactatc ttgatcacga acgacgaatt ctgctccagc aagtgtttgg tctgatgtaa
    14221 cgtcaccatc tactttaaca aatcgtttgc ccccagtaac gacatcgact gacggtggtg
    14281 tttgatcatt tgtatgcccg ttatcgacat ttgcttggtt agaaaatcct ttggttggat
    14341 ctgctttttc gttcaaatgc atatagtaaa cgaatttcaa tgtaccgcca ggagttaatg
    14401 aaggaatata attcggatca actgaaaccg tgaatccgtc tgtttgctca gtgacagaat
    14461 aatttactgg gtcgatttct ttatttccat catataaggc ataagcatac gttcctgaag
    14521 aatcattatc aaatgttaaa gcagcgtcat gagtatcgat aagtttgaat gttgtgtact
    14581 tgttttgcgt tccttcttta tcagcgatac ctaatgggat attgacagaa atctcatatt
    14641 gaattttttc accgattgcg acatcttttc cattcaattg aggtgttgtt ttatcaactt
    14701 tagacgtatc atttttgatg gtcttttcta ctggtgtttg gctatttgcc aggatctcaa
    14761 aaggcgtgat tgtttgcttt tcaatcattt ccgcattatc aggagctttt acttcttcta
    14821 aattatattt tccaacttct aaatgattaa cgatcaactg gcctttttca atagatgcct
    14881 cggcaaagtc attgttgccg atgtcataag aatgaccagt aatgaaatgt ttggctttgg
    14941 tttgatcagt tgtccaagtg tacaatccat ctgtgacact ttggatgtat tttttgacgc
    15001 ttggtgttcc ttcttcttta gaaataataa attctgctcc atttagtgct tcgttttcgg
    15061 cagtaccgat ttttgtaact ttcaacgttc catcattacc gactgtattt ttagggtaga
    15121 gatggatagt atctagttct tctgtcccgt atttataaga gccatctgct tgtttgatca
    15181 tctcatatac agggaaagct aaaaccatgt ttgcggcagc tgacactccg tcttttggtt
    15241 cttctttgat cgtgtagact gcatctttcc cattttgttt tttaggtaaa gataaagtga
    15301 catttccatc agcatctgtc gttcctgaag caactggtgt accaggagtc aaagactgga
    15361 ctgcttgttt tgctgcatcc acggacgctc ctttatctcg ttgcgcataa aattcttgag
    15421 tgacgttata aactgaaaat gaaatatcgg ctaatccttg gtattgatcg aattcgctca
    15481 tttctttccc gctgttttgg attaaaggat cgggtaaatc agtcattttc tttttatgaa
    15541 ggatgacttg agctgctgtc tcctctgcaa acactttttt tgcgccgaag ctgtttgtga
    15601 gtaatggtaa aataaggaaa aggactccta acataacgtt tattttttta tgatttttca
    15661 ttttttcact cctgttcttt ttttgataaa caaccagatt gcaaagcttg cgaataccat
    15721 accaacaacg cctaaaagtg tatttttcgt ttcgtttgtt tttggaagaa atggacggct
    15781 attttctttt ttattgacta ctttcaatat actgtccgcc tctttatcct cagaaagaaa
    15841 agttgaaatg gtaaacggaa tttctgtttc acttaaaata taaccagaag gtgcttcgat
    15901 ctcttttaat cgataacttc ctgttttcaa cccggaaatt gaaaaagcgc catttttatc
    15961 ggaaatcagg tgaagcgcat ctgattcgtt ctttgtccac ttgtatccgt tggctgtttg
    16021 ctggaggtaa tttcctgctt tgtttttaat aaggaatact gcgccagcta atcgtttatc
    16081 tggtttattt gtctcgattt tgacaaattg cttactccct gtatatacag agactttctt
    16141 tgtactgaca ccaaaaccaa aatccagtgt tccgttgttg ttgatttcct tgttcgcctg
    16201 tgctgtactg cttagctgca tctgataaga aatggtcagt ttttttccag catatttttc
    16261 caacgaaggg atcgaaaata aaacatcaaa tccgtgaggt ttcttgtgta tgacaaagcc
    16321 ttctgtcagc ttttctccat tcgatgaaat cgttaaacta ttaggtaaaa acgttaatgc
    16381 ttcatccgca ctatctgaca attcgaactt ttgatagtca aggatattta caggaattgt
    16441 cgtatgcaag gaataagtga ttttttcacc aatcgtaaaa tcatttctag gctcttctaa
    16501 taccttctca aaaggtggat cagggtagtc gttttcttca tttttaggat acaaatggat
    16561 agttgaaagt ttttgattgt tttgtccatg tacaggaaga acaactacca tattctctgc
    16621 cttttctttg acgacttctg gtactttgga ttcaatgaaa agatacgctt tatctctttt
    16681 ttcttgatct ttagcggcca gagaaaaaga agcgatacca tcttcgttgt taagagttgt
    16741 agttgttgct tctgcggtaa acataccgga agacgcaccg ttttttgcga tctctgcttg
    16801 tgcttcttct accgttttgc ccttttcccg tagatggtaa aaagaatctg tgacatcata
    16861 aacttggaat gtgacaccat ttaatcctcg atacgtttgt aataaagctt tttcttgtcc
    16921 gtcatttgga tgatttttcg gcagttgccc gtttgggaaa agcaatttgt gtaaagtaat
    16981 ctgaaccatt tctgtttctg tttctgttgc tacctgagta aaagctggtt taaatagtaa
    17041 caagaagaga cacatactaa gccaaccaag ttttttcata tttagctgcc ccctctttct
    17101 tttttcaaaa aaagatacca aatagaaaac gcacaaccaa tcatccctac tagatagatt
    17161 cctaaacggc caattcctcc cgtttcaggt aatggtactt ttgcctgatt cgtgatgtct
    17221 aaagaaatct ggttgttttt ggctcctggt gacagaacag attcatgatc ttgtccatcc
    17281 acttgaattg acccatcttc gtgtataact atagtaactg gctcttttag accttggtat
    17341 ccttctggtg taaaagtttc ggtcagcgta taagttccag gttttaaatt ttcgaataga
    17401 aaggtatctg tttcttgtcc atcttttggc gattctaagt ccatttctgg tccctgcagt
    17461 ctgaattttg ctccttttaa tgtctgtccg ttgtcttctt ttttgtgtac cgttagatca
    17521 aacgctttta gtcgattttg atgagtgagc gtccatacat tatttgtcat ctcggtattt
    17581 tttcccgtgt aaatgatttt tccatattta tcgatttgga attcataatt tcccggcgaa
    17641 gtatcatatc cggcaggacc agctgtttca acgactcgat atttaccagg tgtgagtgaa
    17701 tcaaaactgg caagaccttt ttcattcgtt gtttggctgt caattggctg ataagtacca
    17761 tttgcttctt ttttctgtag ggaaaatgct gctcctttaa gatttatctc tttgtccgtc
    17821 ccttgcatcg catacttacg tactgctaca ggaacttcaa caaaaggatt ttcaatagtc
    17881 aactggatcg tatcatcgga agtagtgact gtttttccat caatggttac cgtaccatca
    17941 gaagcgatct tgatttccca agtagtcttt ttgcttaatc catgcccttc tggagctttt
    18001 gtttctgtca gcgtataggt catttctttt tgcaatttga cattttctgg aagagaatag
    18061 gttccgtcgc catgatctgt tagtaaagta tcaatagaat ctcctgttaa tttgaaaaca
    18121 gcgccaataa gatccttttc acctgtagag gaatttttcg ttattttcaa gtttaacggt
    18181 gtgaattgtt tagtattttt ccatgtcatt gcatctattt gttgagaatc gtatcctggt
    18241 acaggtaatt ctttgattgt ttggtaactg aatgcttgac cttgattatt gtattgaggt
    18301 agtgataata tctcttgata actttctccg ctatttgcag ataatttgtc aatgtctgca
    18361 cgttcccacg tatttgttgt atctttagct ggtttaatga ttcgaatata tccgtttttc
    18421 caagctgcag catttgtcgt atgttcccgt tgaatctcaa aagtaacttg atctggacga
    18481 tcagctagat tgttgtcaaa ttcttcccat aactttttga tgtgaagact gactccggga
    18541 gctttagcag atggtatacc gaattcagct aattcattgg tatcaatgtt tggctggaaa
    18601 gttgtcctgc cgttcatttg ataccagaaa tttggatgga agtcctcatt ttctgtttgg
    18661 attctcactt ggtaatggat ttggatttct tggtcttttc ctaaatagat ctgattgctc
    18721 ttgatggtat ttccttctat ggaaatagat ggagtgaccg ttgtaggact tgtccccaca
    18781 ctcttgactg aaagtgtacc aggctgataa atgaatggtt ctgcgattgg atcatttatt
    18841 tgtccattgc ttacagttgc tgagatctgt acagcttttt tggctaggta ttcagagata
    18901 tctgttgcat gatcagctga ttcatagtaa agatcgcctt tttcatctga tgaaaccatt
    18961 tgtcgcatac gagactctac ttctgctttt gagagaccag ctgccggatc gctttgaagt
    19021 tggatgccaa gaccatgtat ttcgattcct cgttctttga gtgccatcgc ttctccgatg
    19081 gttgcgataa acgtactgtc gattcttctg gatagattgt tttggtcagg tgcatcatag
    19141 attcttgaga ttagagaagt atttcccggc cgatcttgcg tattagaaaa ctgagttccg
    19201 taataattgc tgcttgattg tgcgtgtacc cgctgtactt tataggaaaa tgttggtaca
    19261 ccatccgtca gcaaaacgat cactttttta tgtccatttg gaacggatag catgcttcct
    19321 gcatctctta gtgctttttg tgtaaaagta ccaccatttg tccgtgaagg tgtaatggat
    19381 tttacttgat ttttcactga atcaaatgaa cccatctgta ctgcaccgtt actgtagcta
    19441 tatccttcgc ttgagtagcc gacatatccc atattgattt tgtctgtgat accgctatct
    19501 gctaaagtat cgacaaaacg atcgacacca atctttactt caccgatccg attattgtcg
    19561 ttcatacttc ctgaccagtc tacgaccaat acgagatcaa gaggcgtgat atctttttgt
    19621 acatttccac gagcattcaa ataaacatca aacaatccgg gtgtacttgt ttcctttgca
    19681 tactttcgta tcgcatagtc tgcttgatta ccggttcctc catattcgat atacgaatgg
    19741 gtccgatcat taggatctcc attccaagaa tttataccat cccattgatt tgttccgttt
    19801 ttattgcctt gatgatcaag aacatttgta tttcctgtag gttgccaact ggctttagga
    19861 taagtgccct gatcatctgt tgtatattct gtaggaatcg tttgattaga tggcacactc
    19921 acagctggca aggataatgt aggcaatgta aatgggctga cagaattttc gttttctttt
    19981 gatgattctt cttctatttc gtctggcttt ggtactgaca catctgtatc tttcttattt
    20041 tctgtctcat caataatgtc atattcttta ttatcgacga tcagcttgtt tgcctggctg
    20101 tggtaaagtg ctggatcgat tggaaaactc agatcgatag tttgtttagt gtttgatgag
    20161 atttccaacg taattttccc gctatttacc tgataagcat agccatctcc ttcttttctt
    20221 tccacatcac ttaagtaatt ttcgactaga ccaatttctt tttctatctt ttcagcactg
    20281 gtgttattcg catttagctt gatgttcagt gctttttttc cagactgatc cgaaatgact
    20341 tcagctgaaa ttgtttctaa aactgttgga tcatctgtag tgtctgctat tgcagataat
    20401 ggcacaaagt ttgataaact caatattatc agcatgaaaa taactttcag tttcttccct
    20461 gtggtcgtca tttttcccct cctttttctt tttttctgtt ttgacgtgaa tagtgaaaat
    20521 tattaaaata aacaggcgtt acataatttt atttatttta atatgataat tattatacga
    20581 agggcgctaa agggaatcac accaattttc ataccaattg gtttcattta tgaagttgat
    20641 tccttcgatt aatgagcgtt tcattgaaag aaaagggttg agggagaaaa acatatgatc
    20701 aggttgttgg aatgaaacta tagaaagatg gaaaaaatca ttcgaattct gtccatttct
    20761 gacaagtgga tgactaaaaa agaacttgcc aaacgaattg gtagttcaga atctacattt
    20821 gtaaggtata tcgaagagat caaaatccgc tggggaacgg atgtaacgat tcaatcttca
    20881 caaaaattgg ggtatcgtct tgaacatttg aatgtaccag cttatttaaa tgttctgatg
    20941 gaaatgtcgc aaagttctac aaacagtcgt ttattaaatg agattattgt caatccgggt
    21001 aaaacgattg agtattattg tgaaaaaatt tccatcagcc ggtcttcttt tacacgtaag
    21061 ttgaaacaat gcaatcaaat tttagaaagc tatttattaa aagtgatcgt tgaccaaggt
    21121 tttcagttaa ctagccttaa aagtgagctc cagttgcgta tctttgttac ctttttcttt
    21181 ttaagttact acggaagacg cacgttgcca tttccaatcg aaaaaaagaa gatcaaagaa
    21241 attatggtca agaataattg tcagatgaat ttgtttcagg aagaaagccc atatgagcaa
    21301 tctttcttta ttatgtattt tgttgtgcat ctcatgagag aatcccaagg aactccattt
    21361 gaatggaaag ctggagactc ttatgtacag ttgataaata tcgataaaaa agacttctta
    21421 atcttgaaaa atctcttagg aaaattgaag tacagcagct atataaaagt agtgaactat
    21481 tttgtcaata cgattttttc agaattttcg gagaaagaaa aaaatgaaat cagtgagcag
    21541 atacgaaaaa atatgtccta ctgttcgttt tcatccgctg attgcaaaga aaaaaacgaa
    21601 gaatatgttg ttcaattatt aacgcaaatt tattttttct cccgtatttt cccatacgat
    21661 acgactcatt tgacgatccg tgcaacttac tttgcacagc agatgcaaca ccgccagata
    21721 cgtctatatg ctggatttcg tgcagaactt caattttatt ccactctact gaatcagaat
    21781 ctgatttatt atttatctag ctatatcttt tggatgctta tcggtaatcc tgaaattcac
    21841 cattatactt ctgataaaaa aatactgatt atcagcgact taagtcttcg acatagtcaa
    21901 tatattgaag aatatatcag tgatattctt gctgtgcata aaattcactc tgagacaact
    21961 gctattactg aagatcagct gtcgaaatac aatcttctcg agtatgatct gatcgttacg
    22021 aatcaaccca ttttgaatgc ccatgttcct catatattga ttgatgatag tgtgtcattc
    22081 gcaaatgaag aagaactcat ccgcttattc gagttatgaa taatcaaaaa acagctatgt
    22141 ataaaccacc ctgataaaaa tttcaggaca aatacacata gctgtttctt tttattgtat
    22201 tccttcagca atccaacgag atttatagcg aaagtctttt aacaactctt cttttgtcca
    22261 attatttgca atgagatccg tcaaagtgtt cattacgagg gtcatggaaa agtataggaa
    22321 actaactgct tcttccgacg attgaatgga gaaatgattg attcttaata attccagcca
    22381 tttttcgttc attggagaat cagccgatag ttctctacgt ttggttgtct gctgatcatt
    22441 tcgctgggtt aacaagtata acctttgcca atagatactt tcgtgatcta agtcgcagca
    22501 ccactctaaa tatttttcga tccccaaaaa gaaatgttgt ttgttttcgc taatgaattt
    22561 taagatatct tgatggacta tgtgagaact gcttttgatc gtatacaaat aagcatcttc
    22621 caaatcttga aaatatttat aaaaagcacc gcgcgacatc cccatcttgt caacgatttc
    22681 tgagactttg acttggctga tgtgctgtga gcaaaatatc tccagtaata ggtcatcgat
    22741 tctctgtttc tttgcgttcg ataaattgaa aaaagtttct tttggcatcc acatctctcc
    22801 tttatctctg attatacgtg gatagccctt cattgtcatg aaggattcat tccttctttt
    22861 tctcttcttc tgtcaagtac ccatcttcca ttcgataaac gcgatcactc cattgaatca
    22921 tacggacatc atgagtaacc ataatagtcg ctttttgttt ctcatgagct tctttaacta
    22981 gtaatttgac gacttcatat gcatgatctg tatccaaact agctgttggt tcgtctgcta
    23041 agatcaagct tggctcatta tataaagcgc gagcaattgc cactctctgt ctttctccac
    23101 cagaaaggtc tttaggataa ctatttttaa gtgactggat atctaaagat tgcagcaagg
    23161 aatcgatttt tgttgaatct tctttttttg aagtaacttg gtcaactagt tcaaattgtt
    23221 cctttaccgt taaaaaagga atcaaattag agctttggag aataaatcct atttctttaa
    23281 agcgtaaatc tgctcgtttt ttttctggaa gctcagttag tgagtgaccg ttgatcacta
    23341 tttctccgga agtcggtgtt tgcaatcctc ctgaaatggt aaggaaagta cttttacctg
    23401 aaccagaagg accaatgatg ctgacgaatt ctccttcctt tacataaaaa tcaattgctt
    23461 tcaaagctga gacctttgta tgaccagaac caaatgtttt ggtaactttt ttcatcgtta
    23521 atattttttt catttttatt aacctcctat tgctgtaatt gggtctactt tggttatcgt
    23581 acgtatcgaa aacaatccgc ctagcattgc gacaatgatc aaaatcccac tatatagcaa
    23641 ccattgtgac caaaaaacag caaaaggcat cgcgcttggt aaaatcagac tagtcaaata
    23701 agcaaacaaa agagcaagca gaacgcctac caccccaaca ataaaggact gtgctactaa
    23761 agatttagcg atgaagaaat ttttgatccc ttgtgctttc attactccaa aaatagcagt
    23821 cttctgaagt gtcatcacgt acatgaaaat accgaccacg gcagcaacca ctaagaacaa
    23881 aaagtatatc atcgcattta aggtcatatt ttgggcagaa tagccaggaa tactttcaat
    23941 gaatgtccca atagttaata actgtagacc cttcgatgct ttttctttcg aaattttagc
    24001 tgaatccttt attacgatcc cattgatcgg ctgttcttct tcagaagcaa acggctgttg
    24061 tccatacttc aaagcagtcc acgtatctag ggacgcatag atgacgggag aaactgtgta
    24121 ataagtttca ggaaagatac cgacaatcgt caaaggatcg tcgtaactgc caattttgat
    24181 cgtatctcca attttatagc ctgaatcagc cagattttga gagatgatga tttcattctt
    24241 tgttttaaat tcatgccctt tcgtgagttt tggtaataaa aatgcttctt tcgttgtgcc
    24301 gaaaactgta acattttctt tgttttttcc tttgattgct ccgctgtaca atccgattgg
    24361 tgctttctct cctccttcaa tgtatttcag atctcctctt gttaattgtg aagctgcaaa
    24421 tacttgattt gcatcctccg acaaaacaat ctcttgtgcg tcccaatcat cgattgcttt
    24481 tttaaactct tctgaaagtc cattcgctag tcctgacaac atgaatacga catacgcaat
    24541 caagaacata ataccgacga tcaagccata tcgtaattta gaatagcgaa tttctttgat
    24601 agctagaaac atttgttttt cctccctatg atagattgat agtgacaaaa tgtcacttgt
    24661 tgaatagtgt aactctaaag tgacaatttg tcacttgtgt tgtttttttc ttatcaataa
    24721 attgtctaag acatctttcg ataaaacctt aacatgagtc ataaaaaatt tatttaagcc
    24781 ttatttatgc tttccatgat aaactaaagt gaccaaatca atgaggtggg aaaatgatta
    24841 aactattagt agtagaagac gataaagtat tatccaataa tataaaagaa atactcgctg
    24901 caatcggcga gataacacaa gtttatgatg gtagtgaagg actgttcgag ttggaaagtg
    24961 gagtttatga tcttgcaatc ttggatttga tgatgccgga aatgaatgga tatgaagtgt
    25021 tagcaaaaat acggaaagaa aaaatacaga ctccagtatt gattttgact gcaaaagata
    25081 gtttggaaga taagattgaa ggatttgaac gaggtgcaga tgattatcta acaaaacctt
    25141 tctatcgaga agaactgatc atgcgcgtga aagcattatt gaaacgctcg ctaggtctct
    25201 ttgatgaaaa tgttctgaag tataaggata tcagttgtga tttaaaaagc aatgaagtct
    25261 tgtataaaga acaagttttg cccatacaag gaaaagagtt tgaattgctc gtttattttt
    25321 tacaaaataa gggaatcatt ttgacaaaag agcagatatt tgaccggatc tggggatttg
    25381 attctgatac gacgattaca gttattgaag tttatatgag caacttacga aaacatctgc
    25441 gaagtactgg attggataaa gaaatcaaaa ccttacgtaa tgtcggttat atactgcaaa
    25501 gggtgtgatg caatgaatgg agatattaca cgaaagcaac gtagtcgctt ttttgcgaca
    25561 aatgtagcag catttgcttt tatctttttt cttttgggat tcattacttt acagatatta
    25621 aaccaatctg catacagaga aacagatctg tcattagaga aaataaattt tgattctcga
    25681 acagtacaat tagagataca aagatatcag caaggcgatc cctttttaaa cccgactcaa
    25741 acggaacaaa acagaacaaa catggaacag ccattaatgg aaccatcaaa tgaccgtttt
    25801 aacactcaag tgatcttgtg gtctaaaaat ggtgagatat tgaataaagc agctattggt
    25861 gggcgtttca accaaatcaa agaattaaaa atggatacga aaaatctgaa taccatccaa
    25921 aatattgaat taaatgaaga cagtggctca tctgccctct ctttccgttc gattacgaaa
    25981 aaagcggaga cgaatcaaga tggcgttgcc tacatccaaa ttttagaaaa tacaaatcaa
    26041 atcaagaatt cattagcgac ttttcgcacg attatgattt tgtgtatgat tgttttttgg
    26101 attctctcta ttggtatcag ttattacttg tctagtctaa gcatgcgccc tattattctt
    26161 tcttggagaa gacaaaaaga atttgttgag aatgcttctc atgaattgcg cacaccgtta
    26221 acgattattc aaaatagttt ggaacatctt tttaccaagc ctaatcatac tattattgaa
    26281 gaatccgaaa gcatcgctca agcattgtca gagacgaggc gcctcactgg attgacttcg
    26341 gatttgctga caatcgctcg gaatgattct gatcagcagc tgctttcaaa gcaaatgatc
    26401 aatacacaag aatatatcga acaacttgtt aaaccatttc aagagatggc catcatagat
    26461 ggaaaagagt ttattctcca taacgatgca acattagaag taccaattga tgaaaaaaaa
    26521 atccatcaag ttttggttat tttgctggat aatgcattaa aatatacgaa tccagaagat
    26581 aagatcattt tggattcatc tgttaaccat aaagaatggc tgattcacgt aaagaatact
    26641 ggcccttcta tcagtgaaga agatcaagca aggatttttg atcgatttta tcgggaagat
    26701 caatctcggg cgaaagagac tggcggatac gggcttggct tggcaattgc aaaacaggta
    26761 atcgaacagc atcatggaca gatttctgta tccaattatc ttccacaagg agtcgatttt
    26821 aaagtccaat tgccgttgaa gcctaaaaaa taatgcttat ccctaaaaaa gggctgtgac
    26881 aaaaatcttg tcacagccct tttttgttgc gaataaacgg tgttccagaa gtagcttctt
    26941 cggaaataag ccgaaatttc tttaagctga ccacttctgt cacaacctct ttagcttacg
    27001 cttacgattt tttattgcgg tcttcaatgt gtacatcacc gcttgtgtca atatccaatt
    27061 cttctcggct aacatcttct gtcacttttt caacatcttc atgttcttct ttatgaatcc
    27121 caacttcttc acgaataact gtatgtttgc ttacatctac ttgctcttct ttgattggga
    27181 tagtaatcgt gtcatcttcc aatttttcgt ctgttccctg tgaagattga tctgttactg
    27241 gtttgcgttc gatgacgatt tcttcacgtt tgaccggtac ttctaccgtt tcagtatcgt
    27301 tgaccacatg tttgtggatg tcgacttctc cagttgtcac atcatgtgta tttacatcta
    27361 atttttcttc ttttagttga attgtatcat catctagatt tggtttgcga tcgatgtctt
    27421 cagtcttgct tgtacccatt gtatcagtct cgccaggtac gtccatcgga tcagttcctg
    27481 gtgtcatatt caatggatcc gttgtatttt gaaagcctgt cgtgccacct acgtccattg
    27541 gatctgttcc agtgccagtt tctaatccag tccccattgt gcttgctggg ctaacagtat
    27601 cttgtgtatc cattgtatct tctgttgttt ctggctgacg atagcctttt actgtgatga
    27661 caaattttcc atcagaaata tctcctctat attgatataa aggatcattt tctggtgtag
    27721 ccgtctcaga atcataggta tcaaaagaaa aagtatcttt tattttttcc cataaagaac
    27781 ggtcttcttc accatccatc cgattttcat ctgtttcaac atctatacca gacaatccgt
    27841 caaatcctag tctagatgct gcttcatcag tagtataaat aacgatatca tctcgttcat
    27901 acccttcgtt acgcaaccgt tcaattactg ataatgcttc ttccctcgta ctatagcttc
    27961 cgatgaccgt taaatttttc tcttccatgt aaattcctcc tagcaaatag atttatgatt
    28021 ggtatattgt tact
//
LOCUS       DAHUVV010000025        27981 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.025, whole genome shotgun sequence.
ACCESSION   DAHUVV010000025 DAHUVV010000000
VERSION     DAHUVV010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.025"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            287..688
                     /locus_tag="NK767_001126"
                     /pseudo
     CDS             287..688
                     /locus_tag="NK767_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            707..1108
                     /locus_tag="NK767_001127"
     CDS             707..1108
                     /locus_tag="NK767_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694134.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1110..1481
                     /locus_tag="NK767_001128"
     CDS             1110..1481
                     /locus_tag="NK767_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694135.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1517..1819)
                     /locus_tag="NK767_001129"
     CDS             complement(1517..1819)
                     /locus_tag="NK767_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694136.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2068..2268)
                     /locus_tag="NK767_001130"
     CDS             complement(2068..2268)
                     /locus_tag="NK767_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCH6694137.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2573..3805)
                     /locus_tag="NK767_001131"
     CDS             complement(2573..3805)
                     /locus_tag="NK767_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCH6694138.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            4059..4628
                     /locus_tag="NK767_001132"
     CDS             4059..4628
                     /locus_tag="NK767_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCH6694139.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4806..5246)
                     /locus_tag="NK767_001133"
     CDS             complement(4806..5246)
                     /locus_tag="NK767_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCH6694140.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5404..6168
                     /gene="map"
                     /locus_tag="NK767_001134"
     CDS             5404..6168
                     /gene="map"
                     /locus_tag="NK767_001134"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCH6694141.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            6200..7123
                     /locus_tag="NK767_001135"
     CDS             6200..7123
                     /locus_tag="NK767_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCH6694142.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            7199..8338
                     /locus_tag="NK767_001136"
     CDS             7199..8338
                     /locus_tag="NK767_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCH6694143.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            8331..9131
                     /locus_tag="NK767_001137"
     CDS             8331..9131
                     /locus_tag="NK767_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCH6694144.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            9131..9958
                     /locus_tag="NK767_001138"
     CDS             9131..9958
                     /locus_tag="NK767_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCH6694145.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9936..10670
                     /locus_tag="NK767_001139"
     CDS             9936..10670
                     /locus_tag="NK767_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCH6694146.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            10770..11636
                     /gene="rfbA"
                     /locus_tag="NK767_001140"
     CDS             10770..11636
                     /gene="rfbA"
                     /locus_tag="NK767_001140"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCH6694147.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11650..12222
                     /gene="rfbC"
                     /locus_tag="NK767_001141"
     CDS             11650..12222
                     /gene="rfbC"
                     /locus_tag="NK767_001141"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCH6694148.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            12244..13272
                     /gene="rfbB"
                     /locus_tag="NK767_001142"
     CDS             12244..13272
                     /gene="rfbB"
                     /locus_tag="NK767_001142"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCH6694149.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13370..14221
                     /gene="rfbD"
                     /locus_tag="NK767_001143"
     CDS             13370..14221
                     /gene="rfbD"
                     /locus_tag="NK767_001143"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCH6694150.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            14256..16289
                     /locus_tag="NK767_001144"
     CDS             14256..16289
                     /locus_tag="NK767_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694151.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            16333..17613
                     /locus_tag="NK767_001145"
     CDS             16333..17613
                     /locus_tag="NK767_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HCH6694152.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCRSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            17823..18629
                     /locus_tag="NK767_001146"
     CDS             17823..18629
                     /locus_tag="NK767_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6694153.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            18641..19861
                     /locus_tag="NK767_001147"
     CDS             18641..19861
                     /locus_tag="NK767_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6694154.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            19851..21437
                     /locus_tag="NK767_001148"
     CDS             19851..21437
                     /locus_tag="NK767_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCH6694155.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            21476..23614
                     /locus_tag="NK767_001149"
     CDS             21476..23614
                     /locus_tag="NK767_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCH6694156.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(23982..24965)
                     /locus_tag="NK767_001150"
     CDS             complement(23982..24965)
                     /locus_tag="NK767_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCH6694157.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            25343..26734
                     /locus_tag="NK767_001151"
     CDS             25343..26734
                     /locus_tag="NK767_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCH6694158.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            26750..27790
                     /locus_tag="NK767_001152"
     CDS             26750..27790
                     /locus_tag="NK767_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCH6694159.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            27809..>27981
                     /locus_tag="NK767_001153"
     CDS             27809..>27981
                     /locus_tag="NK767_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCH6694160.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGR"
BASE COUNT         9004 a         4655 c         5616 g         8706 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaat taaggagacg atacggatgc ttgatatagt
      721 accatcaaat acacatcgga gtggtagctt aataaatttt gctgcgattt ttgcttgcgc
      781 atcggtaatt ctagcatctc cagttgttgt ggatgcaagt tctactccga aaactaaaaa
      841 tgataatcaa cttgtaatta ctacaaaaaa tgagattaac acaatttcaa gtaatgatgg
      901 caaaattttt gatgtgataa atacggtttt aaaaagaaaa gctgaaatca atataaaata
      961 tgatgaagat ttaaacttgt atttctttgt tatcaaaaca acatctgaat tgttttcctc
     1021 agattacgat gttttggata ctctcgataa tgtattatct gactataaat atatggggaa
     1081 atctgtagta gcaactttgg gggaataaaa tgtttgactt taatgaatat ttatctattt
     1141 ccaaacaaat tgaatcagat gataaatata attctaaaga atcttataga agaactgctg
     1201 tttcaagagc atactacagt gcttttaaaa aatctgatga gtatttgaaa gaaaattatg
     1261 atattattta taatggaagt agcggaaaag gaagccacca aaccgtttgg aatttgtttt
     1321 caacagttaa ggaattgaat accttaggaa tacataatag tgggtataga atgttagaga
     1381 aaagaaaatg tgctgactat attcctaatg aaaccattac gaaaacagat atggcgctga
     1441 tgaatcgaga agcagaaaaa ataataaaca aactcaccta atgaatttca agcgagtttg
     1501 tttgttattt ttttatttaa tctttgtatc tttctggatc aacgaaagta tactttatat
     1561 agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc agtcacgata tcaaacaaaa
     1621 agtatacacc cttcttcatt ctagttttcg cagcaggaat tttgaaatag ttcttattag
     1681 aatagtagag attgattaat aagctatctt cgattgctaa aaagaaaact tctgaattcc
     1741 atactttata aaaatctttg ataaatctat tcgaagggtc aaatttaaac cataattgtg
     1801 tttttccttc catcagcata aaagttcacc tcaaaaagag tatacgaacg aatgttcttt
     1861 ttgtgaaggc ggaatttaat atactgaata atagaaaaca agtacttgtg ccaaattatg
     1921 tgccaaaaaa attcgaattc aacaaaatgt aaacaaatag aaaaccttct atattaagat
     1981 ttcctgcaat aacaaacaca acagaagacg tgtaatagtt agtcaggaac gtacaaataa
     2041 cccctgtatc ctttgcggta cagggggtta ttttgtataa gtagacattg tagtttctaa
     2101 actgctatgt cctaaacgtt tcgatacgct aagtatattt actccttgat aaagtaaaat
     2161 agatgcatgc gtatgtctta gtccatcaat aaacagtcga agagaaaata gggaaaaatt
     2221 gattactaaa gaaaaactta ctaggtcgac ggtgtagttg attttcatac ttcaatagat
     2281 tcgaattaaa cgatttcgat tggcttatac cagtcctatc caacgaaaga tatatcgttc
     2341 ttaactctag cggtataact tgaagcttta cgtaaagcca atgggaatga actggagatg
     2401 actttttatt atttttagta atagctgatg ggttaatact tacaactttc cttttcaaag
     2461 taccatataa aaaaacggtg gggttgttta gcgaaataaa ggatctaagc caatagctta
     2521 gatcctttat tctatgcttt atattatttt ttgcatgtgt gcgaaagtat actcaagccc
     2581 aatcgccatt tcggaaaatc ggtactgtag agccatcttc acggattccg tcgatatcca
     2641 tcttgtcaga tcctaccatg aaatctacgt gggtttgact tctattcaat ccagcttctg
     2701 ctaattcttc atcggacatt tctgtaccgc ctttcacact gaaggcataa gcagaaccta
     2761 gtgctaagtg attcgatgcg ttttcatcaa ataatgtatt gaaaaagatg atgccagatt
     2821 gagaaatagg tgaagggtca ggtacaagtg cgacttctcc taaacgacgc gcaccttcat
     2881 ctgtatcaag cagttttgct aagacatctt ctccttgttc ggcagaaaaa tcaacaactt
     2941 ttccatcttt aaaagtaaat ttcatacccg agatgatggt gccagcataa ctcagtggct
     3001 ttgtacttga aatgtaacca tctactcgat gactgtcagg tgctgtgaac acttcttccg
     3061 tcggcatatt tgccatgaat ttttctccac gagcattata gcttccagcg ccttcccaaa
     3121 gatggttttt aggcaatccg ataatgatat ctgtaccggg agcagtatag tgaagggcag
     3181 aaaattgttc tcgattcaat tcttctgctt tttttgctaa tttttcatca tgcttcttcc
     3241 atgctaagac agggtcttct tcatagacac gagtcgtttt gaaaatctga tcccataatg
     3301 cctcaacttg tttttcttct ggcagttctg ggaagacttt tgctgcccat tgtttaccag
     3361 ccgcagccac gacagtccaa ctgactttat ttgcttgagt tgccttgcgt aaattcatta
     3421 gtgctttgcc attcgctgat tgataagacg ctactcgatg gctatctaca ccagcaaatg
     3481 catcgggatc ggcagaaaca acgctgatcc tgcttgctcc tttttcaagc cattcatccg
     3541 cttgatcgat tttagattgt ggtacattct caatacgatc cgttgcagca tgtaaaagaa
     3601 attctctttg gatctggtcg tcagtccatt gaacgataac ctcagcggct cctagtttat
     3661 aagcttcttg tgtgatcaat cgagcaaggg gtgcctgttc cacgctgatc tgtaaaacaa
     3721 ctgtgtgtcc tttttctgtg gctacgccag tttctgcaat taagcgtgca tattttttta
     3781 gaagttcatt aaaatcagat aacataataa aaattcctcc tcaccatttt ttctggtaac
     3841 tattagcatg ataccattta attatgattc aaacaatgag gctttaaata aaaaaaggag
     3901 gttatttttt taaggttaag tttcatgata tatgttacta cttgctattt cagcttgtga
     3961 agaaaatcaa aaactaattt tgcatatcaa aaaaacatgt tacaatgaaa ttaatcaatg
     4021 gtggaagcgt tataaaaaaa ttaaggagat taaaaaatat ggcaagaaaa aagacgatta
     4081 ctagagaaca gattttggca gctgcttatg aagtagctgc caccgatgga tttacacgat
     4141 ttactgcgag aaacatcgcg aacaaaatga attgttccac gcaaccgatt tatcttgagt
     4201 ttaaaaatat ggatgacttg agacaagctc tttttgagca aatctatgat catttacaat
     4261 atgaagtgtt cactgttgag catacaggga atacgattat cgatcttgct tttaattata
     4321 tccattttgc aagaagagag aagaagttgt atcgttcact gtacttagaa gaatatggtg
     4381 gaggaaaaga gatgcagaat ttctcttata cttactttgt gaatacagtg aaacgcgatc
     4441 cagcttatgt gaatctttca gaagagcaga tcgaatcttt gtataatggg acatggatca
     4501 tagctactgg gatcgcagct ttgatgtctt caagcatcat ccatccaact gatgaacaga
     4561 ttgaaaagat gatccaagac agtatagatg ctatcttgaa attcaaagaa ccaatcgaaa
     4621 tcgattgaat atactcaaaa gttccacaaa tcagtaggta acaagagatg tgtcaaaata
     4681 aggaaaagtt cgtttttctt acagcgtatg catttttaac tcccgaagaa ctcttccatg
     4741 aatgcaatga ataaaagagc catacagatt gaataaatct ggatggctct tttattgcaa
     4801 gtatattaaa tttttgcgac tagttcttta gcgaaagctt ctaaattttc gatatcttct
     4861 tcttcagcat tcagatccac tttaacattt tcggctcctt ttgatgcgcc tgtttttgca
     4921 aaagcagcat cgaaatcatc caccgatttg cagaattcat cgtaaaaagt atcaccagat
     4981 ccgcagacac cgtaaatttt tcccaataga tccaattctt gcaagtcttc ataaaagtct
     5041 acgatctcat caggcagatc gccatcatcg tatgtataag tagctactac gcaaatatct
     5101 gcctcctcga attcatctgc atctacttgg gtacattcgt tgatttcgac ttcgatatcc
     5161 agattttcaa atgcttctgc aacaatatcg gcgatttctt cagtgtttcc tgtcatgcta
     5221 gcatatacga tttttgctaa agccatgatg tgcctcctcg tttataatta tttaacaatt
     5281 atgaattata ccaaaacatc attgaaaaga aaataaaacc taaagacacc tggggaatgt
     5341 catttggcta aatgtatgcc gctatgttaa aatgagaggg atttgtaaaa aggagacgaa
     5401 aatatgatta ctttaaaatc agcacgtgaa attgaaatga tggcagaatc tggagcttta
     5461 ttagcagatg tacataaaaa tctgcgtgat tttataaaac caggaatcac aagttgggat
     5521 attgaggtat ttgtcagaaa ctatatcgag agtcatggcg gaattgctgc gcaaattggc
     5581 tttgaagggt atgaatatgc gacttgtatc agtataaatg atgaaatttg tcatggattc
     5641 cctcgtaaaa agcctttgaa aaacggtgat ttggtaaaag tcgatatgtg tatcgatttg
     5701 aaaggcggca tttctgattc ttgttgggcc tatgtagttg gagaatctac acctgagatc
     5761 gaccgattga tggaagtaac gaagaaagct ttgtacatcg gtattgagca agcacaagta
     5821 gggaatcgga ttggtgacat cggtcatgct atccaaactt atgtagaagg cgaaaatcta
     5881 gcgatcgttc gtgattttat tggtcatggc gttggtccta cgattcatga agaaccagca
     5941 gttcctcatt acggagaagc tggaaaaggg ttgcggttga aagaaggcat ggttatcacg
     6001 atcgaaccta tggttaatac cggaacttgg aaaatgaaaa tggatcctaa cggctggact
     6061 gcgtatacaa gagacggcgg actttcttgc caatatgaac atacattggc tattactaaa
     6121 gatggtccaa gaattttgac atcacaagga gaagaaggca cttattgatc tatccagtca
     6181 ataggtacgg gagaggtaca tgaaagtttg gaacaaaata aaacaaaatg aaaatctgat
     6241 gcgctttatt gagacgactc aaaagcgcat cactgattca gaaatgagta cgacctctgt
     6301 cgtcgttgct tattatttac tcttgtcttt atttccattg attattgctt ttggtaatat
     6361 tttaccgttt ctccacatcg atcaagagac tgtactgacc tatttacgag aagtgattcc
     6421 tgagaccgtt taccagttta tcggtcctgc aatcaaagac cttttgacac aaagttcagg
     6481 aggcttgtta tccgtttctg cattagcggc tttatggtct gcaagtcaaa gtatcaatgc
     6541 tttgcaaatt gcgatgaata aagcgtatgg cgtagaaaat agaaaaaatt ttttgatcgt
     6601 ccgtttcttc tcactaattg tcattgtctt gttcatgatt gctattagtg gggtaactat
     6661 cgttcttggg ttgggacagc tgattttgga tgctattcag ccaatcgtac aaatacccgt
     6721 gaactttatc gatcagttcc aaactttgaa gtggccaatc acattggtag ctttatttgt
     6781 catcatgttc ttgatttatt taattgtccc taatgctcag ttgaagttga agacgatcat
     6841 tccgggcacg atcttcgcta ctgtcggctg gatgctgtta tcacaagtgt ttagtatcta
     6901 tgctaggtat tttgcaacga ggatcagcgg ttatcagatc ataggaagtt ttattgtact
     6961 catgctttgg cttaattttg ctgctacgat catcattttg ggcggaatca tcaatgcagt
     7021 tgttcaagaa tacgtgacgg gtcatcaaat caaaaaacgt cgagatccaa gccgaaaatg
     7081 gttcaataaa ttgaaaacta aattttctca taaaaaaaag tagaaacaag agttcttatt
     7141 gtaaatttat atcagtaatt tataataatt atgactctta attaaagtag gtgcccatat
     7201 gcttgtttct tttagtatgg ttataagatt ttttctgaca attttattat cactgatatt
     7261 aacacctgta ttcaaaatca tttcagtaca aacaggtatg gtcgacaaac caaatgaaag
     7321 aagaataaat aaagttccaa tgccttcagc gggcggactg cctatcttta tttcatttgt
     7381 tatatcgact ttattcttat tccgtaaaat tatccctcaa tcgtatattg taccgatctt
     7441 gattgcttct ttagtaatca tagtcacagg agtgctcgat gataaatatg agctgacacc
     7501 taaacaaaaa agtataggga tcttgattgc ttctcttatt atttattttg tagcagatat
     7561 acgtattgat tccttcacgc ttccttttat tggatatatc caactaggct ggcttagttt
     7621 tcccgtaacg attttttgga tcttcggtat cacgaatgcg gtcaatctga ttgacggttt
     7681 ggatgggctg gcggcaggga tttcattgat cggtttaatt acgataggaa tcattggata
     7741 ttttttcctc catgcttcta cagtgtatat tccagttgtt attttctgtc ttgtagcaag
     7801 tattatcggt ttttttcctt acaattttta tccggcaaag atttatttag gcgatacagg
     7861 agctctattc ctagggttta tgatggccgt cctttcacta caagggctga agaacgtgac
     7921 ttttgtttca tcgatttctt tgctgatcgt catgggagtg ccggtaacag acacgttttt
     7981 tgctatcatt aggagaaaag caaaccgtgt ttctttttct acagcagata aaaaacatct
     8041 tcatcaccga ctaatcagtt taggctttac tcataaaggt gctgtcctga cgatttatgc
     8101 gatggctttg atgttttctt tcacagctat ggtcatgaat tatactggaa gaatcggaac
     8161 gatcatattg attattgcca tgttgtttgc tgcaatttta ttatttgaat tgattggtct
     8221 gatcaatgag aaacatcagt ttattttaaa aacattgcga tttttaggga ataaagagta
     8281 ccgtacgcaa gtaaaacaga aatatttgaa gaaatggaac cgtcatatag atgaataaca
     8341 aattaaacca gccgatcctt attagtattg tgacggcaaa cagtgataaa atttttcgga
     8401 cactagataa ttttatttca actgtaaaag atgaaacagc agtaaaaatc cgaatttttg
     8461 acaacaattc acttcccgag tacaaaaaaa gactaacaga gtataggaaa tatccctaca
     8521 tcgatatcta ttttcatgat gaaaattccg gatttggtta tggacataat cacaatctga
     8581 tagctagcga ttctcgctat ggcattattt gtaacccaga tattttagtg gatgaagaga
     8641 cagttacagc attggtcttg ttattgaaag aacatccgga atgtgcgatg ttagcaccta
     8701 agattcttaa tgaagatgga acaactcagc acctcatccg tgagcagctg gctgtttttg
     8761 attacgtttt gcgctttgtg cctttcaaat gggtcaaaaa aaagtttgac aaacgactgg
     8821 ctgtgtttga atgccgagga ttgcctactg atgctaatag ttatgtacgg atgatctcag
     8881 gtagttttat ggtagctgat attgataaat ttaaagagat aaaggggttt gatgagcgtt
     8941 tcttcatgta ctttgaagac aacgacttgt gtttgagttt tgaaaaagca ggttacaagt
     9001 tattgtacac acccttttat gcagttatcc atttttacgg gaagggtgcg catcgaaatg
     9061 caaaactgtt tcgcgtgttt ttgacttcta tgatgaaatt tttcaataag tgggggtggc
     9121 gttttttctg atgaaaccag aaatattagt agtcgttgtg ctttatcagc aactattcag
     9181 tcaatctcca gcttatggac cattaagcaa agcacttagc gaaaagcaaa ttcagcttat
     9241 tatttacgat aatagtcctc aaaggcagca aaatgagctg tttgaaaaag aaaatatcgt
     9301 ttatcatcat gatccgcaaa atcctggttt agcggtggct tataattttg cgttgtctca
     9361 agcagatgaa ggaacacgtt gtctcgtcac tctggatcag gatacgaggc tagtcgacga
     9421 ttattttgaa ctattgcgta aagtaacctt tacagatgaa tgtgttgctg cagttccgat
     9481 gattttttct gggagccgac aagtttctcc cctatattca gatcgttata tcaatcgtac
     9541 agctcaagca gtagaagttg aaggcccaac agctgaacga atcatggcaa tcaattcagg
     9601 tgttgcctgg tctgtcaaat ttctaaaaaa gattggcggg tttaatcctg ctttttcttt
     9661 agattttttg gatcattggc tgttttggaa agtggatcag ttggaaaaaa ctgtggagat
     9721 ccttcctgta cgaatggaac atgatctttc ggttttagac tatgaaaaag tttcaagtaa
     9781 tcgataccgt tctattttac aagcagaaaa tcgattttat catgaatatg atcaaaggca
     9841 tttatccagt caccgacgac aattattgtt aagaacagct aaacagttct tgaccgtcaa
     9901 aaaccggcat atctggcgga tgactttgcg ctcatatgtt aagaattgga aggtgtgaat
     9961 tcgtgcgttc ggtttgtata gctaccttta atggaggcag atatttaaga gagcagttag
    10021 atagtatttt atctcagctt tcagcagaag atgaagtagt catttctgat gatgggtcag
    10081 tagatgatac atgggatata ttaactgaat acgcagaaaa agataagcga atccagcttt
    10141 tttcggggcc tcgtcaagga ttgatcgcta attttggtta tgcgatccag cagacgaaag
    10201 gagatctaat ctttttggca gaccaagatg atgtttggtt ggagacaaaa gtgaaaaaga
    10261 tcactgacta ttttgagcag aatccggatc agctagtagt agtttctgat ttaatcattg
    10321 tggacagcga gctgcaagag atccatgatt cgtatttttc gtttcgaaat tccaaagaag
    10381 gatggcttaa taatgtgata agaagtcact atattggtgc tggtatggct ttccggtctt
    10441 ctttaaaacc cgatattttg ccgatcccgt cagaagtgcc gatgcatgat atgtggattg
    10501 gtttgctggg aggaaagaag actgggttcc taagagaacc gttaactctg taccgaagac
    10561 atgatttaaa cgcgagtgag attgaaacac attctaaatt acggcaaaaa atttcttggc
    10621 gtgttcatct gattacggcg ttgatcaaac gaaaatggtt taaacgttga taaaagatgt
    10681 ttaaatcctt aatatttcca tgacaaatat aacaattgat gagataataa caaagtgaaa
    10741 tttgaaatca aaaaggagag cagatatcca tgaagggaat cattcttgct gggggcagcg
    10801 gaacacgtct gtacccttta acaaaggcaa catcgaaaca attgatgccg atttatgaca
    10861 aaccaatgat ttattatcca atgtctactc tgatgttggc gggaatcaaa gaaattctga
    10921 ttatttcaac gccacaagat acaccacgtt tcaaggaatt atttggaaac ggaaatgatt
    10981 taggactgca gatcgaatat gcagtacaag aaagtccaga tggattggca caagcgttta
    11041 tcattggaga agaatttatt ggagatgata gtgtctgctt ggtattaggg gacaatattt
    11101 actatggtgg cggtctgtct aaaatgttgc aacgagcagc ttcaaaagat acaggcgcga
    11161 cagtatttgg ctatcatgtc aatgatccag aacgctttgg tgtagtagaa tttgatgaga
    11221 atatgcaagc tttgtcgatt gaagaaaagc ctgctcagcc aaaatcaaat tacgctgtaa
    11281 ctggccttta tttttatgac aatgaggtaa tttctatcgc taaaggtatc aaaccttcag
    11341 aacgtggaga actagagatt acggatgtca ataaagcata tttggataaa ggaaaactat
    11401 ccgtagaact tatgggacgc ggatttgctt ggcttgatac aggaacacat gaatcattgc
    11461 ttgaagcatc tacctttatc gaaacaattg aaaaacgtca gaacttaaaa gtggcttgtt
    11521 tggaagaaat tgcttaccgt atgggctaca tcgataagga acaattgctg gcccttgcac
    11581 agccattgaa aaaaaatcaa tacggtcaat atttattgaa tttggccgct gaataaggag
    11641 tccttacaga tgaaagtaac agatacaaaa ttaacagatg tcaaaatcgt agaaatggac
    11701 gtctttggcg atcatcgcgg tttcttcaca gaaagttatt caaaagcaaa atttgcagaa
    11761 catggattga aatatgactt tgttcaagac aaccattcac tatcagctga agctggtgtc
    11821 cttcgggggc ttcatttcca aaaaggagaa gctgcacaga caaaattgat tcgggtagtc
    11881 actggagcag ttttagatgt agtagtggat atccgcaaag gtagcccgac ttatggtcaa
    11941 tgggacggat ttatcttatc tgaacataat catcgacagt tgcttgtacc taaaggatat
    12001 gcccatggat tcgttacttt gacaccaaat gtgaacttcc tttataagtg tgataattac
    12061 tataatgcag aagcagatgg gggcatcgca tttgatgatc cagatttagc gattgattgg
    12121 ccaattgatc catcaagagc gattatgtcg gaaaaagatc accgtcatcc gacattaaaa
    12181 gaatttgaag cagaaaatcc atttgtttac ggagaaatct aagaattaag gagaaatttt
    12241 cttatgaaaa acatcattgt taccggaggt gccggattca tcggctctaa tttcgttcat
    12301 tacgtggtaa acaatcatcc agaagtgcat gtcactgttt tggataaact gacttatgca
    12361 ggaaacaaag agaacttggc cggacttcct agcgatcgcg tagaattggt cgtaggagat
    12421 gttgcagatg cagaattagt ggaccgtcta gtaaaagaag cggacgcagt tgttcattac
    12481 gcggcagaat cccataacga taattcatta aaagatcctt tcccatttgt acaaacgaat
    12541 atcattggta cgtatacact tttagaagct tgccgcaagt atgatgtacg ctatcatcac
    12601 gtatctactg acgaggttta cggcgatctt ccattacgtg aagacctgcc aggacatggg
    12661 gaaggcgaag gagaaaaatt cactgcggaa acaccatata atccttcaag tccatattct
    12721 tctacaaaag ctggttcaga tctgttagta aaagcatggg tgcgttcttt tggcttacgt
    12781 gcaaccattt caaactgttc aaacaactat ggtccttatc agcatattga aaaatttatt
    12841 cctcgccaag taacgagtat cttaagcggt attcgtccaa aattatatgg cgaaggaaag
    12901 aatgttcgtg actggatcca tacgaatgat cattcttcag cagtgtggct gattttgacg
    12961 aaaggcagaa ttggcgaaac ctatctgatc ggtgcagacg gtgaagaaga taataagacc
    13021 gtgatggaat tgatcttaga aatgatgggc caaccaaaag acgcatacga ccatgtaaac
    13081 gaccgcgcag ggcacgattt gcgttatgca atcgattcga caaaattgcg agaagagcta
    13141 ggttggcagc ctgagtttac gaacttccgt gatgggttgg ctgacacgat caaatggtac
    13201 gaagaacatg aagactggtg gaaaaaagaa aaagaggccg ttgaagcagc atatgccaaa
    13261 aatggacaat aatacagagg aaatttcact gtattaatgg aaaaacacat gtgaggcagg
    13321 cgctcgcctg tctctttttt aaaataaaaa tcaaggagcg gataaccata tgtttttatt
    13381 aactggagga aatggacaat taggtacaga attgcgccat ttattagatg aaaaaaatct
    13441 gccatatgtt tcaacagatg caaaagactt ggatatcaca gatgctgaga aaacaatggc
    13501 ttatattaca gaattaaaac ccgatgttat tttccattgc gcagcttaca cagcagtaga
    13561 taaagcagaa gaagaagcaa aagaattaga tgagaaaatc aatgtcgatg gcacaagaaa
    13621 tgtagcactt gcagcaaaag ctgtgaatgc acaattcgtc tatatcagta cagactatgt
    13681 tttcgatgga aaaaacaaaa atgtagaata cgaagtagac gaccaaacga atccactaaa
    13741 tgaatatggt cgtacgaaat tattaggcga aaaagcagta gaagagattt tagaagacta
    13801 ttatatcata cgaacttctt gggttttcgg gatctatgga cacaatttcg tcttcacgat
    13861 gcaacgccta gcggaaaccc gcgacaaact gactgtagta aatgaccagt ttgggcgacc
    13921 aacttggaca cgtactttgg cagaatttat ggtatatgtc atcgaagaaa aagcaccatt
    13981 tggtatttat catttgtcga acgaaaatag ctgttcttgg tatgagttcg caaaagaaat
    14041 attgaaagat acagatgtag aagttgcacc tgttacgtca gaagaatttc cgcaaaaagc
    14101 aacacgtccg cagtattcag tcatgagcct gaaaaagaca gaagcgttag gttttgtcat
    14161 accaacttgg caagaagcac ttgcgcaaat gctggaagct gccaaaaaaa tgaataagta
    14221 atttcctttt tatcaaatag aatttggagc atgatatgga aaaacaaaca agaagtgcac
    14281 gacgaaaaga aaagccaaaa gcgaatcagg cagattcgaa tttggtgctt gacctgtttc
    14341 taaaactccg ctatcttatt ggcgttttag tcatcgtggt catcgttact tttaatttaa
    14401 acggaagttc acttggtgtt tgggataaat atgtgtcgca aagagacgat gggaaaaaaa
    14461 ctgatgtgat ttttggacag aatcgggaag tgcggtcaga cgaatggctg gtgcagacac
    14521 ctttttacct ctcacaagct gaagaggact tttcgttggt gaacgaggat tattcattaa
    14581 acggacaaaa catgatcatt gcgtataatt ctcctgtaaa ggatattacg gttatcggaa
    14641 aaccattcaa ctggggattc tttttcctcg gaagagatcg aggactttcc ttttattggg
    14701 cgatgaaatt agtcgtcatg gtcttgcttg gattcgaagt actgatgata ttaacgaaga
    14761 ggaaaaaagc tctatcgcta atcggtgcat ttgcgctgac attttctcca gcagtccagt
    14821 ggtggttcat gcaacatgtg ggcgatttga ttttctttac tttgggactg atgattgctt
    14881 tttatcatta tttctacaat catgataaaa aatggctacg aacgttgatg atgcttttgg
    14941 tggttatttt cggtattggg tttattttag tgatttaccc agcccatcaa gtgatgctgg
    15001 cgtacttgct gattttttat tttgttggcc tgcttattta ctatagcaag aaagtttcat
    15061 gggatctgtt ggatgtagtg ttgattggag gggcagtttt atttattggc ggagtcatgg
    15121 ttcatttctg gctaacgtca aaagacgcat tgttagcttc tttgaatacc ttatatccag
    15181 gaaaacgggt aagtactgga ggaaagtgga cgattggcga atttttttct ttccttacaa
    15241 attggaaaat tccttttgaa gatatcaact attcgaataa ctctgaagtg gccctgttct
    15301 atcacttttt tccaactgta ttcctagcaa gtccatttgt gttatttggc aaaaaagata
    15361 gcgaacaaaa gttactggga cgtattttga tgattttttg tctgttttct atcttttgga
    15421 ttaccgttgg gttgccaaaa tggctagcag aaatcactct gctgtcttat gttcctccat
    15481 taagagccat ccttactttt agttttgcgg cttgtttatt atcgatctgg tttatcgctt
    15541 atatttggag agaagatgtt attcctattt ggtataaatt cattttgttg gcagtaaatg
    15601 catggtctta tttctatgcg attacgcatt cgaaaatgga taattatttt tcggattttg
    15661 aaaccatcgc agttgctgtg ttgggaacag tgattttagc ttttgcatta tttggctgga
    15721 gacgcttgtt ttacctcgct tttgtcgcct tagttatcgt atctggcttt ttcgtcaatc
    15781 cagtcgtaga aggtacagga gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa
    15841 tcgatcaaca agatcctggt aaagtttggt tgtctgaaga tgaattatat aacttcaccc
    15901 caacattggg agtgaaatcc ttcaatacag tacgctttta tccagatata gctgcatgga
    15961 agaaaatcga tccgaaagaa gaatacgaga agtattataa tcggtatgct catacaagag
    16021 cttttatcgc taaggaagat acaaagttca ttttagatcg tccagatatg ttttcggtca
    16081 cgttgaattt tgaagacgca gaaaaattag ggatcgacta tgtgatcagt aaacgaccat
    16141 taactgatta caaccaatta tatgaagcgc agttcacgca gctatatgga ccggataaag
    16201 acggttatat gatttttaaa gtagaatatc cggattatcc agatgtacca caaacacaaa
    16261 ctcaggaaaa tcagctagtc gtacagtaat aggctagctg agaataatcc tgaaatatta
    16321 gtagaaagga gagtgaagat aattcaagca atcgtacaaa aatttgtcaa caaaatgagt
    16381 gtcggtttct tgcttttgtt gctgacgcta gtcttttttg tttggacagc aggggtaggt
    16441 tttccagcaa ctttgtttat atataacaat agttcattac ttatcgcttt tagtgtggct
    16501 attttgctca ttttaaatgt ttataaagca aaaggcgtcg attttcttta tctaggtgtt
    16561 gcgcttgccc ttttcatttt cttttcttac tttcgggaat taagaaatga gtctttattt
    16621 ggagacttac tgtttccttt gattatttta ttttgtttag taattaaatg gatcgagtgg
    16681 acaaaatggg atcgcggtat ttatctgatt gtctttacgg tcttcctcgg aatcacgtta
    16741 tatcgtgtat ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat
    16801 tcgctttctg atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct
    16861 ttattgatca gtggatttgc cagttctttt gaacgatggt atgttcgaat catcggtggg
    16921 atagcagtcg ttgcagcctt tttaggtatt tgggtctgcc gatctagagg tgcattagtc
    16981 gcgttgattg tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt
    17041 gcgccattta ttgcctacac ggttacgatt cttttagctt tgccgatttc ttatctggcg
    17101 gctgtctctg aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat
    17161 cagacgctag gagaaaaatc tgaacaagtt ttactaggaa tgaaaacatt tatcttccag
    17221 cgaggaaacc aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga
    17281 ttgataggat ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg
    17341 aaagcagatt tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg
    17401 cagtcattta tggaggatac attgacttca tacccatggg ttccgattat ttatatcctg
    17461 ttggctatgg ccgttcatcg atatgatgaa ccagaagtta cggaggaatt ctatgttatg
    17521 gaagaggcat atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc
    17581 gctcattctt ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg
    17641 acaaccgtcg cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa
    17701 acgttagaga aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta
    17761 ccaaaaatat ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac
    17821 aaatgtttaa agatttgtat ttatttatga aggacattta tcagaacaga aaattactgc
    17881 tacagttttc gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat
    17941 gggcgtttat caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac
    18001 tgaaagcaca aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga
    18061 ttccctggtt cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata
    18121 cttatttagt aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat
    18181 ccaatctgta cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg
    18241 gtatatatcc ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt
    18301 tcttgacagg attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc
    18361 aattaattac gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta
    18421 atcagtttga ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc
    18481 aaggttatcg tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt
    18541 tgtatttctg ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat
    18601 tgaaaccaca tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag
    18661 actaaaagat atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga
    18721 agccctcaat ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa
    18781 tgtagaaata aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc
    18841 tacaatgttg aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga
    18901 aggaaaaatc tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta
    18961 tgaaaatatc tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat
    19021 ggtcgatgat atcattgaat ttgctgacat cggcgaccat atttatcaac ctgtaaaaac
    19081 ctattcaagt gggatgttcg ttcgtctagc ttttgctgta gcgatcaatg tgaatcctga
    19141 tattttgatt gtggatgaag cattggcagt aggagatctt gaatttcaac taaaatgtat
    19201 ggaaaaattt acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat
    19261 tagttcgatt cgccgttttt gtgatcgttc ctattggttg aaaaatggaa aagttgtcga
    19321 atatggtgat acgatggaag taacgacgaa ttacgaaaac tttttgaaga aaaaatcagt
    19381 taaaacagta gatcgaaata aaaaccaagt agaacgcttc gcagctccag atatcgtaga
    19441 cgtgacgaaa gccgaattgt tggataaaga taaaaatcca gtggatatgc tggaacaagg
    19501 agaaaaacta tacgttaaag taacctatga agtaaaagac gatacaatca aaaatccagt
    19561 tttaggaatt gctctgcgta cagtcgacaa tttttatgtg tgcggactca atactctact
    19621 agatggtatc aagatccctt ggaaaaaagg tgagaatatc ttttatctgg agtatgaaaa
    19681 gttaggtttg ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac
    19741 ggttcctttg gtatacaaga caaaatacat gaacttgttt gtcagcggtt cttatatcgg
    19801 agaaggcatc gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg
    19861 attttgaaat ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac
    19921 caggaagtaa agttttagag tttggtcctg gaaacggccg attaacgaaa catttgatcg
    19981 gagcaaaaca atgtgaagtg agcattgtcg agctagataa ggaattgttc gattttgtca
    20041 gcgagtttgc ccaagatgga ttttatggag atatcgaaag ttttgaatgg gcaaactatt
    20101 atgctggaca aacatttgat tacgttttgt ttgccgatgt tttagaacat ttggtagatc
    20161 ctggaaaaac gttgaagaaa gttagagaat ttttgaatga agaaggagaa attctaatta
    20221 cattccctaa ccttgcgcat aattctgtca tgattgattt gttcaataat caattgccat
    20281 gggcttctta tggtttgcta gatgaaaccc ataattcttt ttatactcat gatggcttcc
    20341 aaaaagtttt tgaaaaagca ggattataca tcaatattga agattatttg tacttagctg
    20401 taggtgatac agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgttatgatt
    20461 tcaaaatgcg accatttggc gaagtctacc aatacttctt ctcattgatg aaacatccag
    20521 tagcacaaag ttccattgcg cagccgcaaa attctaatta tgtgaaagta ttagaagtaa
    20581 tccagcaaac gaaacaagat gaaataattc aacaaatacc attcaataat ttcacagggg
    20641 aaaatgagac actaagtttc catgtatcag aattagcaga acgaatggtg tttcgcttcg
    20701 cgcagcaacc tagctttatc gaattcagcg cagaagctgc tggagaaaaa ctcacattta
    20761 tcgattcaaa tgctgtagtc aaaacagtaa atgattgtta tttgtttgat ggtaaagatt
    20821 taccagagtt ctttttaacg gatattgcag ggaaagaagt gactatccat tgtcactatc
    20881 gttttatcgg agaattgaca ccaacaatga aagaattgct ggaaacaatc cgtccaatgg
    20941 ctgaagttac aaagcagctt tctgagaaaa atgatgaatt agaaagagaa aatcattatc
    21001 ttattgagaa taatacaaga ttagatcaga cgttgaaaac aaccataaat cgctattgca
    21061 cgttgttgga aagtgatgaa tggacaatca aacatagatt agggcgacgt aagaaagaaa
    21121 catcgaaaaa aatccaagaa aaagagcttt ctctatgtat tgacgaaaaa atctgggatg
    21181 cagaaacaaa gatattgaaa atcatcgggt ggggaatcgc taattcggat cgccaaccat
    21241 tgtcctataa gttatcagcc gaccaatcac cattttttga agcaattcct gttttacgag
    21301 aagaagtcaa tcaagcaaaa cagctagctg aaggagcaaa agcaggattt gaactgcgga
    21361 tcctttgcga gaaggaacgt tcattcttag ttgaagcagt ggctcaaaat ggtcaatcat
    21421 ggatcattga aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca
    21481 aaaaaaagaa attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt
    21541 gcttattgtg ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga
    21601 aaaagaaaat gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata
    21661 taatcttgat gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg
    21721 aaaagcaatc ttagacttcc aaacagcaag acatcaaaat ggcatagctg tgaaattaaa
    21781 cggtaaatat ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact
    21841 gaaaaaaggg ataaattatg caaggactca tggcgtaaaa aaagcaatcc gccgggtcaa
    21901 actggaattg aaaccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc
    21961 tgatctgaaa gagcaaagga aagaagtaca aggatttgcc taccgcccat tgatttccat
    22021 acttatgcct gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact
    22081 tgatcaaacg tatgatcact gggaactatg tatcagtgat gatgcttcta ctgatccagc
    22141 tatccgaaag tgtctagaat cctatcaagc aaaagatgat agaattaaag tcgttttccg
    22201 acaagaaaat ggacacatca gcctggcaac taattctgca ttggaaatgg cagaaggaga
    22261 gtttatagcg ctcttagata atgatgacga attacctcca tttgccttat atgaagtggc
    22321 aaaagtgcta aacgtacatc cagaactgga tttgatctac agtgacgaag ataaaatcga
    22381 tgcagatggg aatcgctttg acccgcactt caaagcagac tggtctcccg atacattgat
    22441 gggaaataat tatatctctc acttaggcgt atatcggaca agcattgtta aggaacttgg
    22501 cggtttccgt aaaggatatg aaggctctca agattatgat ttggttcttc gtgtgacaga
    22561 acaaattcct gcagaacaca tttaccacat tgaccgtgtc ttgtatcatt ggcggacgat
    22621 cccgggatca actgcaagca atggagaagc taaaagctac atctatgatt ctggagtgaa
    22681 agcattgaca gatgctctat ctagaagaaa tatcaaggga agcgtacatc ctggcagaat
    22741 ttctggtttt tatgaaatca cttatgatgt tttgcaagaa gacttggtca gtgtgatcat
    22801 tccaactaag aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac
    22861 gtcttatcca aattatgaaa tcattattgc agataatgga agtacagatc ctaaaatgca
    22921 agaattattt gcagaataca agcatcagct gaaagaccga tttatcgtcg aattaattga
    22981 cattccattc aattattcac gtatcaacaa tctggcagca gaaaaagcaa acggcaaata
    23041 ctttttattc ttgaataacg atacagaagt gattgaacct gactggatga cagcgatggt
    23101 ttcttatgca cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga
    23161 tacaacgcag catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa
    23221 taactatgat cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt
    23281 agcagtcact gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt
    23341 tgatgaaaca ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact
    23401 gggtcgttac aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag
    23461 aggttatgaa gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca
    23521 agataaatgg ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga
    23581 aggaatcggc gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt
    23641 gattcgctcg cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg
    23701 actcaaaaaa ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa
    23761 ttcacgaaaa ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt
    23821 ctgctcccac cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc
    23881 cacgctctta ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga
    23941 accctagctg tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt
    24001 ttaaggtatt cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg
    24061 atggagataa ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt
    24121 gctgaatata tttcgttctc agaatctaat acccaccata aatagccata tctcatattt
    24181 tgtaagtctt ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt
    24241 acgatttgtt ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga
    24301 tcacttgccg ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac
    24361 cagacattgg ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca
    24421 gcataataat tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa
    24481 tcaacagttg ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca
    24541 gtttgatagt tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt
    24601 tttgtccaat cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt
    24661 gctttcattg ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc
    24721 actttatact ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc
    24781 tctaaagcaa tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa
    24841 gtatcgtctt ttttataacc attccattca tctgtataaa cttctacccc atccttatat
    24901 gcgacaattt gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta
    24961 ttcataagat attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa
    25021 ttttttcgat tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga
    25081 tggattctct gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac
    25141 ttcgacttct ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt
    25201 agagtccttg tcttgattag aggtgtataa agctttttat tagacatttt taaattccat
    25261 tacaatttgc ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta
    25321 ttttgtgatt aggagtgtat atatgaataa aaatggagaa tggagtgctg cacgacggat
    25381 attcattata ttaatggatg ttattgtata caatgcagca atatatagta gtttcttact
    25441 gaagtttcgt ggagagatcc cattacgaaa tttagaaact ttccaacact cagcaattat
    25501 tatttcagtg atttttattg cgttgaacat tttgttgggt gcatatgttt tttataatcg
    25561 aatgatcagt gacatcgtgt ttgtcacagt catcggtcag gtattgatga cactagggat
    25621 catggtagtg acttttgcgg gccgctggtt tgcttttcca cgtcttgtta tcttactttc
    25681 atttgttctc ggtacgcttt tcttaagtat ctatcgtatt ctcgtatata aactttattt
    25741 acgcgtaagt cgagataaaa aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc
    25801 aatccaaaat tttggttcaa agaaaaacaa caaacataaa gtcgaagcag tcattcttga
    25861 taactattat gcaaacatca aaaatatgat taaagacatc gatattgtct atcttgcttc
    25921 tcatatcgaa gaaactgaaa aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa
    25981 attgtttttg aatacaacat ttgaaaactt gacattgatc aatccaaata ttatgaattt
    26041 cgaagatgaa agtattattg aagcatctgg atttagaatc caacctgaat atgaactatt
    26101 caaacgtttg tttgatatca tcgtttcact tgttttatta gttattgctt caccatttat
    26161 gttattcaca gcaattttag tcaaagtgac atcaccggga ccaatcatct ataaacaagt
    26221 gcggatcaca aaaaatcaaa aggaattttc aatctataaa ttccgctcga tgaccgccac
    26281 agccgaagca aaatctggac cagttttagc taagagcaat gatgcacgag taacgcctgt
    26341 aggtaaattt attcgagctg tccgctttga tgaattacca caaatcttca atgtgctgaa
    26401 aggcgatatg tcgatcgtag gaccacgtcc tgaacgtcct ttcttcgtcg atcagttcaa
    26461 tgcagaaaac ccttattact acttacggca taatgttcgt gctggtatca ctgggtatgc
    26521 acaagtctat ggaaaatatg tctctgatta caacagtaaa ttacgttttg atttacttta
    26581 tatcaaaaaa tattcattga tgcttgattt aaagatcttg ctgcaaacca tcaagatcct
    26641 atttgataaa atgtcctctc aaggacttga agaagacgaa aatgtgatct cattggcaga
    26701 aattgctttt ccgaatgaaa aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc
    26761 gaaattcagc ataatcgtac cagtatacaa tgcagaagca actattggtc gaacgattga
    26821 tattctaaaa gaattggatt atgaagagta cgaagtcgtt ttagtaaatg atggatcaac
    26881 agatcaaaca caggaaatca ttcaagaact gattgaaaat gatgaacgtt ttgaattgat
    26941 tactacgacc aatcaagggc ccggattagc aagaaacgaa ggaataagac agtcaaaagg
    27001 agaatatctc ttattcttcg atgcagatga cacgccagta aaaacgatct tgtcagatta
    27061 tgatcatttg ctagaaaaga gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg
    27121 tacaatggac gagggaagaa tcgtttccga aaaagaaaac ttagttacag aatgtcacta
    27181 tctaactcac gaagagttta taaaagacat gtatcagtta atgaatgaac agttgatgta
    27241 tgttgtatgg aacaaatgct atcgtagcga tattctgaaa aataaccaaa ttctatttaa
    27301 aacttataat agttgtgagg atcgaatatt taacttacat tattatgaat actgtcatca
    27361 agtgatcatg aacccaaaag tagaatatat ttatgaattt gaaggtggga aggggatcac
    27421 taatcaatac cgacctaaca agttcgaaac atttaaagaa ttctattcat tagcaaatac
    27481 tgtaacagac gatattaata aagacggtat ggcggcgctt cttttaaaag ggacaacttc
    27541 agttattttt tctatttata gtacctctgc aagaacttca aaagagaaga aagacgaagc
    27601 aaagcagata ttgagtgatc cagtgatccg agaagccaag caaattgcta agacggattc
    27661 ttcagcaaaa aaaataacaa agttgcttta caatatgcca gctccactat ttttatctgc
    27721 tgtaaaagtt ggaagcttcg ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg
    27781 agtatattag ttaatggagg aaaatataat gaaaagaatt ttgcattttc agggacgaat
    27841 gggtttaggt ggagcagagt ctttcatgat gaatttatat cgtaaaattg atcgtacaaa
    27901 ctatcaattt gattttttga tttatgaaga ttatgcagat gttcaagact atcattcaga
    27961 gattgaaaga ttaggaggcc g
//
LOCUS       DAHUVV010000026        27953 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.026, whole genome shotgun sequence.
ACCESSION   DAHUVV010000026 DAHUVV010000000
VERSION     DAHUVV010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.026"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            25..696
                     /locus_tag="NK767_001154"
     CDS             25..696
                     /locus_tag="NK767_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCH6694161.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="NK767_001155"
     CDS             755..1195
                     /locus_tag="NK767_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694162.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="NK767_001156"
     CDS             1207..1875
                     /locus_tag="NK767_001156"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCH6694163.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="NK767_001157"
     CDS             1939..2562
                     /locus_tag="NK767_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HCH6694164.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="NK767_001158"
     CDS             2618..3004
                     /locus_tag="NK767_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HCH6694165.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="NK767_001159"
     CDS             3217..4608
                     /locus_tag="NK767_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCH6694166.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5219..5587
                     /locus_tag="NK767_001160"
     CDS             5219..5587
                     /locus_tag="NK767_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCH6694167.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5767..6708
                     /locus_tag="NK767_001161"
     CDS             5767..6708
                     /locus_tag="NK767_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCH6694168.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6777..7508
                     /locus_tag="NK767_001162"
     CDS             6777..7508
                     /locus_tag="NK767_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCH6694169.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7652..9832
                     /locus_tag="NK767_001163"
     CDS             7652..9832
                     /locus_tag="NK767_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCH6694170.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9852..10334
                     /locus_tag="NK767_001164"
     CDS             9852..10334
                     /locus_tag="NK767_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCH6694171.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10350..11111
                     /locus_tag="NK767_001165"
     CDS             10350..11111
                     /locus_tag="NK767_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCH6694172.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11242..12576
                     /locus_tag="NK767_001166"
     CDS             11242..12576
                     /locus_tag="NK767_001166"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCH6694173.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12883..13368
                     /locus_tag="NK767_001167"
     CDS             12883..13368
                     /locus_tag="NK767_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HCH6694174.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13361..14044
                     /locus_tag="NK767_001168"
     CDS             13361..14044
                     /locus_tag="NK767_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCH6694175.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14170..16815
                     /gene="polA"
                     /locus_tag="NK767_001169"
     CDS             14170..16815
                     /gene="polA"
                     /locus_tag="NK767_001169"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCH6694176.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16860..17696
                     /gene="mutM"
                     /locus_tag="NK767_001170"
     CDS             16860..17696
                     /gene="mutM"
                     /locus_tag="NK767_001170"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HCH6694177.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17660..18289
                     /locus_tag="NK767_001171"
     CDS             17660..18289
                     /locus_tag="NK767_001171"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCH6694178.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18341..18673
                     /locus_tag="NK767_001172"
     CDS             18341..18673
                     /locus_tag="NK767_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCH6694179.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18985..19476
                     /gene="nrdR"
                     /locus_tag="NK767_001173"
     CDS             18985..19476
                     /gene="nrdR"
                     /locus_tag="NK767_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCH6694180.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19499..20872
                     /locus_tag="NK767_001174"
     CDS             19499..20872
                     /locus_tag="NK767_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCH6694181.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20872..21798
                     /gene="dnaI"
                     /locus_tag="NK767_001175"
     CDS             20872..21798
                     /gene="dnaI"
                     /locus_tag="NK767_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCH6694182.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21927..22367
                     /locus_tag="NK767_001176"
     CDS             21927..22367
                     /locus_tag="NK767_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCH6694183.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22511..23212
                     /locus_tag="NK767_001177"
     CDS             22511..23212
                     /locus_tag="NK767_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCH6694184.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23209..24330
                     /locus_tag="NK767_001178"
     CDS             23209..24330
                     /locus_tag="NK767_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCH6694185.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24345..25577
                     /gene="pepT"
                     /locus_tag="NK767_001179"
     CDS             24345..25577
                     /gene="pepT"
                     /locus_tag="NK767_001179"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCH6694186.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25709..26617
                     /locus_tag="NK767_001180"
     CDS             25709..26617
                     /locus_tag="NK767_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HCH6694187.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26652..26924
                     /locus_tag="NK767_001181"
     CDS             26652..26924
                     /locus_tag="NK767_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HCH6694188.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26935..>27953
                     /locus_tag="NK767_001182"
     CDS             26935..>27953
                     /locus_tag="NK767_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HCH6694189.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEV"
BASE COUNT         9391 a         4835 c         6097 g         7630 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagga ggaaacagat
     5221 gtttacgaat gaaataaaaa tcatgcttta tgtggacgat gtagaaaaaa atgcaacgtt
     5281 ttggagagca gttggctttg cggaaaaaga tcgtcaagaa atggatggga cgcttataat
     5341 tgaagtagga gttgaggaca gtcaaacctc atttctgtta tatgatcgag catttgttga
     5401 gtcccattca cctggcacag ctactagtgc cccatctttg atgttttaca gtaaagatat
     5461 ctttggacta tataaaaaaa tgcagacgca atcagtcaca ttaggtgata tggtacagct
     5521 tggagaagaa tatgtcttta actttgctga tcccgatggc aactactttg cggttacagg
     5581 taaataatag aaataaagac tttgtttttc tgtgcgaaac aggaaagcga agtcttttta
     5641 attcaaataa taatattatg taaacaaaaa actttccatg cgtaattata cacaagcaaa
     5701 taaatcgtca aatgcgaacg ccttctttat actgtttagg aacgaaaaga aggaaggaag
     5761 aaatacatgg attatcaagt actgctttat tacaattata caaaaatcga agaaccagag
     5821 gaatttgcca aaaatcattt agccttttgc aaatctttgg atcttaaagg tcgtattcta
     5881 gtagctgaag aaggaataaa tggtactgtg tctggaaccg ttgaaaacac gactgcctat
     5941 atgaaagcaa tgaaagctga tcctcgattt gctgatacga tgttcaagat cgatccaagc
     6001 gaaggacatg cctttaagaa gatgtttgta agataccgtc cagagcttgt aagtttaaag
     6061 ctagaagagg acattgatcc aaaccaagtg acaggtgcct atctttcacc gcaagaattt
     6121 aaagaagcta ttttagatga acagacagtg gtgattgatg cacggaatga ttatgaatat
     6181 gacttgggac attttcgagg ggctgtacgc ccagatatac gcagttttcg agagcttcca
     6241 gactggatcc gcgagcacaa agaagaattt atggaaaaac gggtagtgac ctactgtact
     6301 ggtggcattc gttgcgagaa gttttctggt tggcttgtcc gtgaaggatt caaagatgtc
     6361 ggacagctgc atggcggtat tgctacttat ggtaaggatc ctgaagtcca aggagaatta
     6421 tgggacggca agatgtatgt gtttgatgaa cggatcgctg tcgatatcaa ccaagtagat
     6481 aaacaaatcg tcggtcgcga ctggtttgac ggaacaccat gtgaacgata tatcaattgt
     6541 agcaatcctg agtgcaatcg tcagattctt gcttccattg aaaatgaaga aaaacattta
     6601 ggagcatgta gcatagagtg tgcgaaacat ccgcgcaatc gctatgttca agaccatgga
     6661 atctctccag aagaagcaga actgcggttt acagctttaa ttaaatagaa aacaatagtt
     6721 ttgattgtcg accatttcca atatgcgcta tactagcgtt gaggatggtg atccacatga
     6781 aatcattgat ttatgctatt ggggatgtcc atggcaaaca tgagcttttt gaaaaactta
     6841 ttcactatta tgatccaaag atccatcagc ttgttttatt aggggatctc aatgatcgag
     6901 gtcctcgtac aaaagaatgt tttttattag gaaagcaatt agtcgaagaa acaggagcca
     6961 tttacctccg gggaaaccat gaagaatatt ttttgcaatt tctgcaatca ccggaggact
     7021 ggtatgcgcc ttatatgaga aatggtggaa aagaaacgtt tgaaagcctg attcacccag
     7081 gggcatcgga ggaatactcc ccaactgaaa tctcgatgat gatccaatcg agatattcag
     7141 aactgattaa atttttagtc cagcgcccct tgtattatga atgggaaaat tatctttttg
     7201 tccatgccgg tgtggatttg acaaagaaag actggcgtaa gacaagtcta agagacttta
     7261 tttggataag ggaaccattt catgaaggga aaaacaatac aggtaagaca attgtatttg
     7321 gacatacgat tacaccaatg ctccatggag atatgcagac aacagacctc tggattcagg
     7381 atcataaaat cggaatggac ggtggggctg tattcggagg gtcattgcat ggggttgttt
     7441 ttgacaagga aggtattctt caagatattg aatatcagaa caacgaagga ccttggcatg
     7501 gaatctgact aatggaaaga atgaaggagc tgtgactttg ttgcggctct ttttattttt
     7561 atagagaata ttgtttcttt cagtagttta acccaggata aaaagtatga tagaataaag
     7621 aagcaatttt tagcagaaaa gaggcatttt aatgacggaa aatttgaacc agacgatcat
     7681 tcagcttgta caaaaagaac tttcagacta tcgtcctaag cagttgacta ctgtacttaa
     7741 tttgttaaat gaaggaaaca ctgttccttt tatcgcgcgt taccgaaaag aaatgactgg
     7801 aagtcttgat gaagtccaga ttcgtgaaat cgaagagcgg tatgcctatc ttgaaaactt
     7861 agaaaaacgt aaaactgaag tgattcggct aatcgatgaa caagggaaac tgactcccga
     7921 gttagaagca gaaatcaccc aagccgtgaa aatgcaacaa gtggaagatt tgtatcgtcc
     7981 atacaaacaa aaacgacgga caaaagcaac gattgctaaa gaaaaaggac ttgaaccact
     8041 cgcgaagtgg ctgatgcagc ttacagatgg agaggtgcaa tctgaagcag aaaaatatat
     8101 tgacaaagaa aaagaagtct cttctgccga agaagcgttg cacggtgctc atgagattat
     8161 tgctgaacaa gtcagcgata atgcaaaatt tcggacttgg attcggtctt atacgtataa
     8221 caaaggtatg tatgtcagca atgtcaagga tgagcaagca gatgaaaaag gcgtatatga
     8281 aatgtattat gactttgcag aacccgtcca taaaatggtt tctcatcgaa tattagctac
     8341 taatcgtgga gaaaaagaag atattctgaa agtattcttt caagtagatg aagcagcaat
     8401 tttagcttac ttggatcgcc aaatagtcaa aaatcctgcc tctccttctt cttcattcgt
     8461 cagagaagcc tatcaggata gttataaacg ctttattcag ccagcaatcg agcgagaact
     8521 gcgcaatgaa ttgactgaaa aggcagatga gcaagcaatc gctattttcg gcgaaaacct
     8581 ccgtaatctt ttgttgcaac cgccactaaa aggtaaagtt gtcctgggat ttgatccggc
     8641 ttatcgtact ggatgtaaac tagcagtcgt agatgcgaca gggaaagttt tagcaatcga
     8701 agttatttat cctcataaac ctgcagcgca agcaaaaaga gaagctgctg gaccagcttt
     8761 tatccaatta attaataaat atcaagtaga tatggtagct atcggaaatg gtacagccag
     8821 tcgggaatct gaactttttg tggctgagca gttgaaatcc gccgaccata aaacctatta
     8881 cgctatcgtc aacgaagcag gcgcttctgt ttactccgct agcgaaattg cacgaaaaga
     8941 atttccgcat ctacaagttg aagaaagaag tgctgtttct attgcccgtc gcctgcaaga
     9001 tcctttagct gaattagtca aaatcgatcc taaagctgtc ggagtaggcc agtaccaaca
     9061 tgacgtttct caaaaacgtt tggctgaaca attggatttt gttgtggaaa cagcggttaa
     9121 ccaagtaggt gtggatgtga atacagcaag cccgcagctc ttgcagcata tatcaggatt
     9181 gaataaaaca actgcccaaa acattgtcat ttatcgtgaa gaaaacggag aattcaccgc
     9241 acgaactcaa ttgaaaaaag tgccacgctt aggaccaaaa gcatatgaac aagcaatcgg
     9301 attcttgcga gtccctggtg ggaaaaacat tttggacaac acaggtatcc atcccgaaag
     9361 ttattctatt gcaaaagaaa tattaacttc agtccaactt tcagaaaaag agcttggtac
     9421 agaagaagca gtagaaaaat tgacacgatt atccgtagag aaactggctg aaagcctatc
     9481 agtcggagaa gaaacgttag ccgatatttt agctgggctg acacaacctg gacgagatat
     9541 gcgtgatgag atgccagcgc ccttattacg tacagatgta ttgagtatgg aagacttgaa
     9601 accagggatg gaattgactg gaacggtccg taatgtcatt gacttcggcg catttgttga
     9661 tatcggtgtg aagcaagacg gacttgtcca tatatcgaaa ctcagcaaaa aattcgtcaa
     9721 gcatcctaca gatgtcgtgt cagtaggaga tatcgtaaca gtttggatag aacaagtcga
     9781 tacgaaaaaa ggcagaatca gtttgacgat gctttcccct tatgaagagt aacaatgagg
     9841 agtaacaaaa attgacacaa gaagaactgc agcagttagt agaagagata tcattaaaaa
     9901 gcttccagct tcctttcaag caccaagcaa tgttcaatcc acgcttacgt acaactggcg
     9961 ggcgctatca tttaaacgat catcatttag attttaatgc gaagctattt gaagagtcag
    10021 acgaagagac gattgcagga atcatcaaac atgaactttg ccattatcat cttcatctag
    10081 ctggaagagg gtatcagcat aaagatatgg atttcaagta tcttttgaaa aaaacaggag
    10141 ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa gatggagtat tatcgatgcc
    10201 aagattgttc tgccacgatc tgccgcaaga gaagaatcga tgtacgcaaa tatcgttgtg
    10261 gaagatgtca tggagaaatc aaatggattg agacaaaaag agaagacttt aatgaagcac
    10321 aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag tgagcagagg aatctaaaag
    10381 actggaatca gctattaaat tttttagccg cacctgaaga gggaaatttt gaggaactga
    10441 cggcacctac acttatttta gctggaaact gtcttcctat cttagctgat aaagcagcac
    10501 aaatgtattt aaatggccaa gtagatcaat tgtttttggt cggaggggtg gggcatgcga
    10561 ctagaatcct ttatgagaat tttgaaaagc aaggatttca ttttgaagat ggcatgagtg
    10621 aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga tttgcccgat aaggcttttc
    10681 tgatagaatc gaagtctaca aattctggag agaacgccac tttttcactg gagattcttc
    10741 attccttagg agctgtacca gaaaaaatct tattgatgaa tgatccgaca ctgcaacgca
    10801 ggactcgagc aacttttgag aaagtatggc aaaatgaaca aactattttc gcgaatgctg
    10861 tgccatttgt gccggaaatc cttcactttt ctgaagagat catctttaca gcaaaagaac
    10921 tgaatcatca atggcctaag gaatatttcc atgcgctagt attaggagaa atggaacgtc
    10981 tccatgacga tgagaacggc tatggtccga aaggaaaaga ttttattcct cacattgata
    11041 tatcagagga agtttggtct tcttacacca gaattaaaga gtcaataaaa acggattttt
    11101 ctcgtacttg accattttag caagcgaatc ctcctttcca aataaatttg gttgaaattt
    11161 tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa tagctaaaag aatggacgct
    11221 agataagaga agaggattca aatggaaaat caaaataaat tgtatcattt tgttggtatc
    11281 aaaggttcag gaatgagctc cttggctctt gttttacatg aacaaggtct aaatgtccaa
    11341 ggatcagaca ttgaaaaata tttctttaca caaagagatt tagaaaaagc aaatatcacg
    11401 attctgccat ttaacgcaga taatgtaaaa ccagggatga cgatcattgc aggaaatgca
    11461 tttcctgatt cacatgaaga aatccagcga gcaaaagaat taggtttgga agtgattcgc
    11521 tatcacgact ttattggtca ttttatccaa aattatacga gtatcgctgt aacaggctct
    11581 cacggaaaaa caagtacgac tggactgctc tctcatgtat tgagtggtgt ccgtccaaca
    11641 agttatctaa ttggagatgg aacaggacat ggtgatccgc aagcggaatt cttttcattt
    11701 gaggcctgtg aatatcgccg tcatttcctt gcctattcac cagactatgc aatcatgacc
    11761 aatatcgatt ttgatcatcc ggattattat acaagtatcg acgatgtatt tacagctttc
    11821 caaacaatgg ctggacaagt gaaaaaagct atttttgcgt atggtgacga tgcttattta
    11881 cgcaaattaa aagcaaatgt gccgatttat tattatggtg tcacagaaaa cgatgatatc
    11941 caagcacgaa acatcgaacg cacaacttct ggttctgctt ttgatgtcta tcatggggat
    12001 gaatttgttg ggcacttcac tgtaccagca ttcggtaaac acaatatctt aaatgcttta
    12061 ggtgttattg cagtagctta tttcgaaaaa ctggatctga aagaagttgc agaagaaatg
    12121 ctgactttcc caggagtaaa acgtcgattc agtgaaaaga tcgtagcaga catgaccgta
    12181 gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa cgattgatgg cgcacgccaa
    12241 aaatatccag ataaagaaat aattgcagtt ttccaacccc atacgtttac gcgtacgatt
    12301 gcattgatgg atgaatttgc agaagcactt gatttagctg ataaagttta cttatgcgat
    12361 atttttggtt cggctcggga agaacaagga aacgttaaga ttgaagacct tggagcgaaa
    12421 atcaaaaaag gtggagaagt catcaaagaa aataatgtat ctccattgtt agattatcat
    12481 gatgcagtag taatcttcat gggtgctgga gatgttcaaa aatttgaaca agcgtatgaa
    12541 aaacttttaa gcagcacgac gaaaaatgta ctgtaataca attactgcag tgtccttata
    12601 aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc cccttttttg caacttatga
    12661 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aatcaaatca aaaacatgag
    12721 aagctgtttt ttcttggctg ttcttattat ttgatgcagg acagcttttt cacgacctct
    12781 gtaaactata ggaatctgtt aaatcaaggg agcagaactt gccttttacg ctacttttgg
    12841 tacaataatg tatgattaaa aataaactgg agggtttcta tcttgaacat aggtaagccg
    12901 agaaaactag gaaaaacaat aaatgaaatc gaggaaggcg actcgttatc cttaacagaa
    12961 tctattgaag ataaccagct tttgctgtat ttgggattga cgaatgatgc aaatcctttg
    13021 tatatccaac atgactttgc acaaaaaaca gaatatcaac agccaatcgt tccatcagtg
    13081 atgctgatgg ggatcatcac aagtgcaatt tcaaagcatc ttcctggacc aggatcacat
    13141 gttgtcaatt tttctgtcaa ttttattgaa ccggttttcc attatgaaac actgactttt
    13201 cagtttgagg tcataaaagt agataagatg aaggatgtgg tgacgatttc tgtagaagcg
    13261 gagaatacga acgaagatcg tgtgttggat gctgtcgtta tggtccaacc gccacaagtt
    13321 cctgaattgg aaatagcaat cgaaagtgaa ggtgaattga atgaataatc caagtacatc
    13381 aatgtcaggt atcaatcgtt tttatgcaaa agtttatgga tttttaggta tgggacttgc
    13441 ccttagtgca gtcacttctt acttggctct ttgggtattt cctttgcaat tagcaacttt
    13501 tatcaataat tttccactag gctttgtggg tctgtggatc gttgaactga ttttagtaat
    13561 ggttctaggc gtaaaagcac aaaagaatcc gagcttagca atcggtggat ttattgccta
    13621 ctctcttttg aatggcataa cattagcggt tactcttgca tactacgata ttggaacagt
    13681 aacgcaagct ttcttgacag ctactttgat gttcatgggg atgtctgttg tcggagtaac
    13741 aacaaaaaga gatcttagcg gggttggaag agcaggacag gcagctttaa tcggagtgat
    13801 cattgcacta gtcttgaaca tcttcatttt gagaagtagc gtagttgagc tgttcatctc
    13861 cctactgtta gtcgtcatct ttgcagggat tacagcttat gataaccaaa gcatccgtaa
    13921 tatctatgtc cagacaaacg gaacagcaca tagtggaatt gctgtattca tggcattgaa
    13981 gctgtatttg gactttatca acttgttctt ggcgtttctg agaatctttg gacggaacaa
    14041 ctaaaaaaga aaaaaattca tcagttgggg ctataccgaa ttatttggta tggtcccgct
    14101 ttttcttttc gcattccgca ggattctttg ttagaatgaa taagaacttt acagtaagga
    14161 gttaagaaga tgacaaaaaa taaattatta ctcgttgatg ggaacagtgt ggcttttcgc
    14221 gcattctttg cgcttcacaa ttctcttgaa cgtttcaaaa atagaaacgg tcttcataca
    14281 aatgcaatct atgcattcaa tacgatgttt gaaaatgtta tgcaaaaaga gcaaccgact
    14341 catgttctgg ttgcgttcga tgcaggaaaa acgacttttc ggacagaaat gtatgcggag
    14401 tacaaaggtg gaagatccaa aacacctggc gaattcaaag aacaaatgcc atatatacgt
    14461 gatttgttga caggactagg ggtacagtat tacgaattgc ccaattatga agcagatgat
    14521 attatcggta ctttggcaga aaaagtagac aaagatcaat ttgatgtcgt cgtgctttca
    14581 ggtgatcggg atttgactca attagctagt aaagaagtaa aagtggatat cactgtaaaa
    14641 ggtgtcagcg atatcgaaag ctatacacca gaacatgtag cggaaaaata tgatgggttg
    14701 acgccaaaac agattattga catgaaggga ctagccggag atgcctctga caatatcccc
    14761 ggtgtgacta aaatcggtga aaaaacagct atcaagctat tgaaacaata tggctcagtc
    14821 gaaggaattt atgaacatat cgatgaaatg aagcaaagca aaatgaaaga aaatctgata
    14881 aatgacaaag agcaggcttt cttatcgaaa aaattggcga ccatcaatac ttcagcgcct
    14941 gtggatgtct ctatcgactc attgaagtat gaagggaaaa atctcgataa actcgttccg
    15001 ttttataaag aaatggattt caatcagttc ctttctaaat tgaatatcgt agaagaagaa
    15061 gtaaaaatgg atgatatttt gtttgaagtc gttcatgaat tcaaagagga aatgtttact
    15121 acggatatgg cgttgtacgt ggaaatgatg ggcgacaatt atcatacaga ggaaatcgtc
    15181 ggagtagcgt gggggacaga aaagaaaatt tatgtcacga acgatctatc tgtattcgaa
    15241 aaccgagcgt tccattcttg gatcactgat ccgactcggc tgaaaaaagt atatgatgcg
    15301 aaacgaactt atgttgcttt aaaccgctat actggaaaaa cgaaaggaat cgattttgat
    15361 gtcttgctag gtgcatacct tctcgatacg aatgaaaaaa gcacagatat cgaaggaata
    15421 gctgctcatt acggatacaa cgatatccaa tcagatgaag ctgtatacgg caaaggggcg
    15481 aaaaaaggtt taccagaaga agaggaactc ttttttgctc atttagcgcg aaaagtagct
    15541 gcaatcaatg cgttgacacc aaaattgagc caagaactgg cagaaaaaaa ccaagaagat
    15601 cttttccgga aaatggaact gccattgtct atcattttgg cagagatgga aataagcggg
    15661 atcaaagtag atgcgactcg cttacaagaa atgaagggcg agttctctgc tcgcttaaga
    15721 gagatcgaac aaaaaatcta cgaagaagcc ggcgaagaat tcaatttgaa ttcaccaaag
    15781 caattaggag tgatcttatt tgaaaaaatg ggattacccg ttatcaaaaa aacaaaaact
    15841 ggatattcca cagccgtaga tgtactggaa cagcttcgtg aacaagcacc aatcgtagag
    15901 gatatcctca cctatcgtca aattgcaaaa attcaatcta cctatgtcga gggactgctg
    15961 aaagtgatcc aatcagatgg aaaagtgcat acccgttatg tccagacatt gacccaaaca
    16021 ggacgattaa gctctgttga tcctaatttg cagaacatcc cgattcgatt agatgaaggg
    16081 agaaagatac gtcaggcatt tgttcctaga gaaaaagact ggctgattta ttcttctgac
    16141 tattcacaaa tcgagttacg cgtacttgcg catatctcaa atgatgaaca tttgaaagct
    16201 gccttcctag aaggtcaaga tatccatgcc agcacagcga tgcgtgtatt tggaatcgaa
    16261 aaggcagagg atgtcacacc aaatatgcgt aggcaggcaa aagcagtgaa ttttggtatc
    16321 gtgtatggca tctctgatta cggactttca caaaatctag gaatcagccg aaaagcagca
    16381 cagcaataca tcgatacgta ctttgaaaaa tacccaggtg tcaaagaata tatggaaaga
    16441 atcgtacgcg aagcaaaaga ccaaggctac gtcgaaacgc tgtatcaccg ccgtcgatat
    16501 ctgccagaca tcaattcaag aaattacaat atccgttctt ttgcggaacg aacagctatc
    16561 aatacaccaa tccaaggaag tgctgctgat attttgaaaa tcgcgatgat cgaattggat
    16621 aaacgattaa aagaaacagg tcttcaagct acgatgctgt tacaagttca tgatgaactt
    16681 gtcttcgagg taccggaaaa agaacttgaa tcattagata aattggtgaa ggaagttatg
    16741 gaacaagctg tttctcttca tgtaccactg atcactgaca gcagttgggg aaaaacgtgg
    16801 tacgaagcaa aataaacatt gtttaagaca gagcaatata atgaaggtga aaagaaaaaa
    16861 tgccagaatt accagaagta gaaaccgttc gtaaaggatt ggaacggtta gtagtaggaa
    16921 aaacgattca aaaagttcag gtcttgtggc ccaagatcat cgagcaacca gagacaccca
    16981 tctttgaggc ttctcttgtt ggcgaaacca tccaatccat tggacgtaga gggaagtttt
    17041 taatttttca tctcgatcat tgtgaactga tctcacactt gcgaatggaa ggaaaatatc
    17101 aatttacgaa agaaaataca ccaatcgata aacatacgca tgtacttttc ttttttgagg
    17161 atggaagcca acttcgctac aacgatgtac gaaaatttgg tcgtatgaca atcgtcgaaa
    17221 aaggagcaag tgcaacttac cgaggaatca tgaaattggg acctgagcct ttgccagata
    17281 gcttcttatt agcagatttc gcaaatggac tgaaaaagtc ccacaaagca atcaagccat
    17341 tgctgctaga ccaacgtcta gtgactggtc taggtaatat ttatgtagat gaagcattat
    17401 gggaagcgaa gatccatcca gaacaaccgg cgaacacact gcgccccaaa gaggtagaac
    17461 aattacgtct atcgatcatt gatgttttgg atcgtgcaat agaagccgga ggtaccacga
    17521 ttcgcagtta tttgaatgca ttaggagaat caggcggatt tcaagtagca ctgcatgtct
    17581 atcagcaaac tggcaaacca tgtatacgtt gtggaacgcc gatcgtgaaa acaaaagtgg
    17641 cacaaagggg gacgcactat tgtccgaatt gtcaaaggct aaaaatcgca aagtaggatt
    17701 tgttttaggt ttgacgggtg gaatagctac cggtaaaagt acggcagcca aagtctttca
    17761 gtctcatgga tttcctttga tcgatggcga tgtgattgcc agagaaactg ttgagcctgg
    17821 aacgcttgct ctgaggaaaa ttgtatcagt gtttggccaa gaggtattgc agcctgatgg
    17881 gcaactagat cgagggaaac ttgggatgat tgtttttcca tcaaaggaaa aacggcaaaa
    17941 acttgatcag ctattagatc cgtttatccg aaaagcgata aaagatcaaa tcgcctctct
    18001 aagttccaat catcctttag taattgtaga tatcccattg ctctacgaag gtcattatga
    18061 ccactatatg gatgcagtag ctgtcgttta tactacgcca gaaactcagc ttcagcgttt
    18121 gatgaaacgt aatcaactga cgctagaaca agcgcagcag agaattagta gtcaacttcc
    18181 aatcgaagaa aaaaagcagc gtgcggatat tttgttcgac aacaatggaa caaaagaaaa
    18241 acttgtggga caaatcgaaa attggctttt tgctaatcgc tttctttgag tatctgtgtc
    18301 aattttgcta tcctgagtat atagaaagtg agggatagcg atgggatcat taaaaaatca
    18361 aaaggataaa gtcgtaggta acgcaaaaga aaaaatcggc gagatgctca ataaagaaaa
    18421 tctaatcgat gaaggtcgta cgcaatcaga aacagcagaa gaaaaagaaa agcagtacca
    18481 agcaagaatc gatgaaatag aagaccagca ggcagaagaa gaagcgaagc ggcaagattt
    18541 gactcaagaa cgaaaaaatc cgacacctga tcgtagtaaa acaccgctcc cagaaagtga
    18601 ccacattccc gatgagcaaa aaatcgagga acagcgaatg aaacactata caggaatcga
    18661 agagaagtta taaaatagta aaaaatcgag atgtgacttg aagcagatga gaaggtgttc
    18721 atctgcttca agttttttgt gctagatgac taaatcagct tttgtgtaag ttatttctca
    18781 gtaatcaagg tgatcagtat tggaaaattg aaatttagta aggacagggg acacaattac
    18841 gcatgaaagt agaaaaaatt gttcttatat ccgtgttttt tgggtaggac agtcgcgttt
    18901 gtgatttgtt tcgtgttata atacaactag ttgtaaagaa agacacatcc tttatataaa
    18961 atgcgatgca agaggtgaga caaaatgaga tgtcccagat gtcaacataa taattcacga
    19021 gtaatcgaca gccgtcaagc agatgatgga cgtgcgatcc gtcgccgtcg cgaatgtgag
    19081 aattgttcgt tccgattcac tacatttgaa cgaattgaag ctgctccgtt attagtaatc
    19141 aagaaaaacg gggaacgcga agagttcaat cgcgataaaa ttttaagagg actcatccgt
    19201 tcagcagaaa aaagacctgt agctatggaa caaatggaac aaatcgtcaa ccatgtcgaa
    19261 aaccgcgtac gcagtctcgg agaaaacgaa gtatcgacga atctaattgg tgaatatgtg
    19321 atggaagatt tagttgattt agacgaaatc gcttatattc gttttgcaag tgtttatcgc
    19381 cagttcaaag atatgtctgt tttcttaaaa gagttgcaag atatcgtgga taaagcaaaa
    19441 gaaaataata aagagaataa taaagaaaac aattaagaaa caaaaaggag gcatttcctt
    19501 ggaaaatgct tggaaagagc ttcaaccaaa aaatatttac caaggaagaa aagcggctcc
    19561 gctgagagaa gacagcaaga acgccttgct gtatctttat cagccactaa tcggcggaga
    19621 agccttgagt atctatttca ccctgttatc agaaatctct caagaatctg gcaaaggtcc
    19681 tgaagggatg catgcagatc tgcttagtag tctaggctgc ggtatcccgc aattttatga
    19741 tgcaagaaaa aagctagaag gaatcggctt actggatgtt tattttaaag aagatagtga
    19801 tctgggacct cgttttattt atgagctgtt agaaccactc attcctaaag catttttcaa
    19861 agactctgtt ctggcttttc ttcttctgga aaaagttggt gaacgacgat tccagaagct
    19921 tacccaattc tttgaaccta aaaggcatac agcagaggga tatcaaaaaa tcacaaaaaa
    19981 atttttagat gtttatcgct tcaatgaagc agtgtacgct gctaatcaag aaaacatcga
    20041 atcgctgaat aaatcttttg aagaatctga atcagtagca ttacttgaag cagattcgac
    20101 attcgattgg cagtttttga atgattggct ggcaaaaaaa catattgcaa gtcttgatga
    20161 tccaatcatc aaacaagtcg acatgtatca tcagctttac ggtttcgatg aattgacact
    20221 tggcgaactg atcagtcaat cttatgactt tagtgaacaa aaagtatctg aaaaagaact
    20281 gcaaaaagcc attttggcaa tgcagagacg tccggtgaag caaacaaaag aaacagttgc
    20341 ggtttctgaa aacagtaatg aaccctcgtc aaacgaacat ttgacaaacg cagcgcttca
    20401 gctgataaaa gaagcacaga gcattccacc gatgaaatat ctggaagcaa tcaagtcaga
    20461 aaaaggcgga tatgtttcta aacaagaaaa ctggttactg caagatcttg tttctcaatc
    20521 aggtctttcc agtagcgtga tcaatgtttt gctgaattat gtactagtga tcaagaataa
    20581 tgctagctta aatgccagtt atgtaaatac agtggcaaat gaatgggcac aaaagaaaat
    20641 cgccacagca gaagaagcga tccagcacat tcggcaaata agtaatcaag caaaacaatc
    20701 aaaacaaaag aagcaaacaa attatcaaac tggcaagcga aatgttcgac gggaaaagct
    20761 tcctgattgg gtcaaccagc caaaagatga aaaacagatc agcagcgaga aaaaagcaga
    20821 gattgaccgc cgttttaaag aatacctggc aaaaaaggag ggtgatatct agtggaagac
    20881 gtaggcaaag aattgaagag gatcatcagc aaacgcaatt atcaagaaaa gtatgctgaa
    20941 atgatcgctg aagtcttaaa tgatgcggat gttcaagctt ttctaagcgc acaccaagaa
    21001 gaattgacac aagcggatat tgaaaaaagt tacgctaaat tgtatgaatt cgtgcaagaa
    21061 aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc ccggctatga gccgaagctg
    21121 acgttgaatt ttcattatgt ggatgtcact tatgtaccaa cggaagcatt gttagcacgg
    21181 caaagacaag aagaaatcag aagccgagta aaagcaatgg atatgccaaa agatattcaa
    21241 tcggcttctt ttgaaaactt tgaacgtaca gatggacgag gatatgcgag cttagaagcc
    21301 ctagattttg tagagaaata tactgctgat ccaaaagggt atcataaagg actgtatcta
    21361 gtaggaagtt ttggtattgg aaaaacatat ttattaggag caacagcgag agaattagcg
    21421 attgctggat tcaccacgac gctagtccat tttccgacat ttgccgttga gatgaaacag
    21481 gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag tgaaacgttc acctattttg
    21541 atgatcgatg acattggtgc agatgcgatg agtagttgga tacgagacga agtattcggt
    21601 gttatccttc aatatcggat gcaggaacag ttaccaacct tcttctcatc gaattttaca
    21661 atggatgaat tggaacagca tttgtccgtc actcaacgtg gagatgaaga gccgctaaaa
    21721 gcgaaacgga taatggaacg catacgttat ttgacaaaag aaatcgaaat gactggtcgc
    21781 aaccgacgca atggttgaac agttgacaag tccaactgac atttgaccta gtagtaaaag
    21841 caggtcagcg aatcattata tggacagaaa tgaccggaga caatcaccgc aacaagtaat
    21901 gcagaaactt aaacggagga ttttttatga gggctatttt ttctagtata tctgtagttg
    21961 gtctattttt tcttttagca ggctgcggta gcgcagatag caacacaggt tctgttgatt
    22021 cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa aacggagaga caaagcatat
    22081 ttgtcggaac gattgaaagt atcactcaag tagaagcaga agaagaaacg cttcagttga
    22141 aactcgtcga tgtcatggcc aaagaagatc cagcgaatat gggaacgtca ttcagcaatg
    22201 acggagtgat cttaaatgct actgctgaaa aaataagcgt ggacataaat gaattgaaaa
    22261 tcggtgacaa agtccaattt acattagttg agatgccgat catgacgatg tctatcccac
    22321 cgcaagttcc tggagattcg attattgatg tttcattgaa caaataacca ataacatttt
    22381 ttcaaaaata agaaaatgtt gttttataga acgaaaaggt atatttttta aggagatatg
    22441 ggaatttttt tattcctagt ctccttattt tatttggaaa atgctataat tatctagaag
    22501 gagtgaagct atgaaccatt tagatttatc tcagcgctta gccgcagtgg gtgactttgt
    22561 ccctgaatcc gctcgtttag cagacattgg atcagatcac gcttacttac ccgttgcatt
    22621 gatattaaga ggaaaaatcg aatatgcagt tgctggtgaa gtcgttcaag gtccttatga
    22681 atcagcacgt aagcaagtca aaaaagatgg attagaagac cgtatcaccg tacgccttgc
    22741 aaatggtcta gatgctatcc agccacaaga tggtattaca gctgtcacga ttgcgggtat
    22801 gggaggttca ctgatccgag atatcctaga agctggcaag ttgaatcaac gcttaagcgg
    22861 acaagaacgt ttgatccttc agccaaacat cggagagcgt acattacgat cttggctgca
    22921 agaaaatcag tataaaatcc aatcagaaaa aatacttgaa gaaaataaaa aaatatatga
    22981 aatcattgta gccgaaaaag aacaaaaacc agttacttac aatgaaatgg agctgatgtt
    23041 cgggccattt ctgcttgaag agaaaagtga gatcttcaaa tcaaaatgga caagagagct
    23101 tcaacaaaga aaagctatcc gtgaacagct caaacaagca gctaatcaag aggcacgtca
    23161 aaaacaaatc gagcaggaaa tccaaatgat tgaggaggtt ttaaagccat gacaatcagc
    23221 ggacgcactt tcatccaaaa gtttgaaagc tattgtcctc agtggctagc tgaagaagga
    23281 gacccagtcg gactgcatat cggaacattg gataagccaa tccaacgagt aatgatgaca
    23341 ttagacgttc gtccagaagt ggtcgaagaa gcaatcaaga aaaaaatcga tttgctgatt
    23401 gctaaacatc ctccaatctt tcgtcctgta aaacggctgg ttactgatca gccacaagaa
    23461 aaaatgtatg cagatttgtt gaaacatgat attgccgtct atgcagcaca cacgaacatg
    23521 gatattattt gggatggact gaatgactgg ttttgtgaac ttctcggtat tgaagtggag
    23581 agttatttag taaagactca tgagattcat tataaaaaac tggccgttta tgtacctgtg
    23641 gatcatgcac agaaaatgcg tgaagtatta gctgcagctg gtgctggtac acaaggtgat
    23701 tataccggta caagttttac ctcgattgga cacggtcgat ttactccgga agctggtgca
    23761 caaccagcga ttgggaaagt agggaagaca gaacaagttc aagaggcaaa agttgaggtt
    23821 attttgccgg aaactatcga aaaacaagtg attcaagcca tgcggtcagc tcatccttat
    23881 gaggaacctg cttatgatct tttcgctata gatgaaccag tcgaaatgtt tgggttagga
    23941 cgagtaggtg aattgccgca agaaatttct atagaggcat ttgtagaaca agtaaaagaa
    24001 gcgttccaac tagatggact gagaatagtc cagccaaaaa atgcaaaatc ctctgtgaag
    24061 cgcattgcga tttgcggtgg aagcggtgaa aaattttatc cgcaagcaat cgcccaaaga
    24121 gcagatgtct atatcacggg agatatctat tatcatacgg ctcatgatat gcaaagcgct
    24181 gggttgattg caattgatcc cggtcattat attgaatcgt tatgcaagca acgatttatc
    24241 gagaaatttg aatcttggaa acaagaagaa aattgggata ttaacttttt cgtttctgaa
    24301 acaaatacaa acccatttca atttaattaa aaggagagaa tactatgtac gaaaatcttt
    24361 tgccacgttt tttacgatat gtcaaaacag aaacacgctc cgatgcaaca agcaaaacga
    24421 ctccatcgac tcagacacaa gttgcttttg cgcaagaatt gaaaaaagaa ttagaagagt
    24481 taggcttagc tgatgttttc tataatgaag ataacggatt cgttatggga acattaccaa
    24541 gcaatgttga tcatgaagtg cgttccattg gatttatttc ccacatggat acagcagatt
    24601 ttaatgcagt aggagtcaat ccacaaatca ttgaaaacta tgatggcgag tctacgatcg
    24661 tactagatgc ggaagaacgc tatacattga atacaaaaga ttttccaaat ttgaaaaatt
    24721 atgaaggcca cacattgatc acaacagatg gcagtacatt actgggagca gatgacaaat
    24781 cagggattgc ggaaatcatg actgcaatgg agatcttgat caataatcct tctatccctc
    24841 acggtaccat caaagtggct tttggtcctg atgaagaaat cggtgtcgga gcggataaat
    24901 tcgatgttga gcaattcgat gtagattttg cttatacagt cgatggtggt cctttaggtg
    24961 agttgcagta tgaaacgttt agtgctgctc aagcaaatat tacgatccaa gggaaaaatg
    25021 tacatccagg gacggcaaaa gatacaatga tcaatgctct acagttagca atcgattttc
    25081 ataatcagct tccagccgat gaagttccag aaaaaacgga aggatacgaa ggtttcttcc
    25141 atttgatggc attggatggg aatcctgaag aagcttctat gagttatatc attcgcgatc
    25201 atgatcgtga aaaattcgaa gcacgcaaag cacaaatcac acatatccaa gaagagttga
    25261 atcagcgatt tgatcaagaa cgaatcaaag tagatatgta tgatcaatat tacaacatga
    25321 aagaaatcat tgaaaaagat atgagcatcg tggaattagc gaaacaagca atgatcgagc
    25381 ttggtatctc tccagtgatc gaaccagttc gtggaggaac agatggttca aaaatctctt
    25441 acatgggtat tccgacgcca aatctatttg ccggaggaga aaatatgcac ggacgattcg
    25501 aatttgtttc tttacaagta atggaaaaag caacagacgt gattgtaaaa attgcagaat
    25561 tgaacacaaa gagttaaaat cgaaaaagaa cagcttatga caaggcgttc gtcccaatga
    25621 tggaatgtca tctgtctaag gctaaagggc aggacaatgg aattggttgt cccgccctct
    25681 tttttgaaga gtaaatagga ggtaaagaat ggaaaagctg ctagtttttt ataacaaaag
    25741 ttcagggaaa gatgagggtg agaaattagc acgctggttt caagattatg taaaagaaaa
    25801 gcagccagaa ttatatgtag aactagctga aactggccca tcgattgatg atgcggaatt
    25861 agtcaagaaa gcagaagcca tagaagctga tactttagta attataggtg gagacggcac
    25921 gattcatcat atcgtccaag cgttccaaaa aacattatct caataccatg tcggattact
    25981 tccaggtgga actgtcaaca accttgcacg tgttcttgag attcccctca aaaaagaaga
    26041 agctgctgac gtgatcctgg aaggcaatgt acggaaaata gactatggtc aagtcaatga
    26101 tgaagtcatt atctctacac tgacaatcgg aatcttagca gatacagcag tttggatcag
    26161 tcaagaagaa aaacagcgtt acggctcgtg gatctttatc aagaagtttt ttaagttatt
    26221 gctgaagaag aaaaaatatc atttggattt agaaacagac gaggagcatt ggaaaggaaa
    26281 atcccaactg ttgacagtca cgatgtctaa ctccgtgggc ggttttacaa attttgacga
    26341 ctcagcgacc ctagatgatg gaaaatttca tgtaacgatc gtaccttcct tagatatttt
    26401 ccgatttatt ttttatttgc ctagaatcat gagaggcaaa ttctatacga tccccggcat
    26461 tacttatttc acagccaaac gcatgaaaat ccaagcgcag gaaaaatctg tgggcactcg
    26521 aacggatgga gacacgacag atgatcttcc agttgaatta tctgttgtat caaaaggatt
    26581 aacagtttat tcaccaaaga aagaagaaaa agcttagaaa tagtagttaa cagaaagttg
    26641 gaagaagagt aatggatgta ttgttacaca atcctttgtg gttttacctt ttcattgtga
    26701 atttttactt attttgtctg atggggtatg ataaacatca ggctagaact agcggctggc
    26761 gtgttcctga gaataatttg atatttgtca gcctgttagg cggtggtttg ggaggattga
    26821 tctctcgaaa agttttccat cataaaactc ggaaaaagaa gtttactata ggattcgctg
    26881 caggtgtact tgttgatttg attctcattt attttttcca ttaaggagca ggagatgtcg
    26941 tctaaaggaa cgaaaactaa aatcattctg aatctgcttt tgctgattgt gatttttgcc
    27001 attatttatt atttaatcaa tcaatcattt gcagatatct ttgccgaatt gctgtctacc
    27061 agtgttgttg ttttggcagc ggctgttata ttagggacag tttaccaaat cgctgaaggg
    27121 cgctcaatca aagaaattgc tgaacctttc gcgccgaatt ttacaacgaa agatgggttc
    27181 ttgacctcta gctatattgc gttttaccga atcgtttcgt ttggtacagg aacgctgatc
    27241 tcggagattt atttttataa caaaaaagga atgaagctat cccagggagc aggggtgaca
    27301 gcactccata tgattatgta caaactggca gtgatcactt atgcattagc tggcttggtc
    27361 atccaattct ctctttttta ttctaatggt ccatcaatga tctggtttat catcgctggt
    27421 atccttttga cgatcgtgat tgttgccttt ttattggcga tgtccgtcag cttgaatgtt
    27481 caaatatttt ttgtcagctt atcgaataag ttatttaaaa gtgagcgttt gcggaaatgg
    27541 gtggatacat gcaatcagca gatctactct ttaagggaaa cggtccaaac cattatccaa
    27601 gacaggactg cgctattgcg gatttatcta tggaatatgt taaaacttgt attttggtat
    27661 gtacttcctt atttgattct gattgaaaac catcctaaaa tagattttct actgaccatt
    27721 tcgtttatca gctttgcagt agtgctttca ggggttattc ctacgcctgc tggtattggt
    27781 tcctttgaat ttgtctattt gttattattc agaccagttg ttggaacagt tgacgcagtt
    27841 tcttcgcttt tactgtatcg tttcagttcc tttatcttgc catttctcta tggatttgtg
    27901 tatgtgattg ctgaacggcg aagagtgatc aaagaagaaa taatagaagt aaa
//
LOCUS       DAHUVV010000027        27676 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.027, whole genome shotgun sequence.
ACCESSION   DAHUVV010000027 DAHUVV010000000
VERSION     DAHUVV010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.027"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            246..2012
                     /locus_tag="NK767_001183"
     CDS             246..2012
                     /locus_tag="NK767_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HCH6694190.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2114..3373
                     /locus_tag="NK767_001184"
     CDS             2114..3373
                     /locus_tag="NK767_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCH6694191.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3520..4821
                     /locus_tag="NK767_001185"
     CDS             3520..4821
                     /locus_tag="NK767_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6694192.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4824..5675
                     /locus_tag="NK767_001186"
     CDS             4824..5675
                     /locus_tag="NK767_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6694193.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5688..6503
                     /locus_tag="NK767_001187"
     CDS             5688..6503
                     /locus_tag="NK767_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HCH6694194.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6654..7820
                     /locus_tag="NK767_001188"
     CDS             6654..7820
                     /locus_tag="NK767_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCH6694195.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8050..8697
                     /locus_tag="NK767_001189"
     CDS             8050..8697
                     /locus_tag="NK767_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCH6694196.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8781..9725
                     /locus_tag="NK767_001190"
     CDS             8781..9725
                     /locus_tag="NK767_001190"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HCH6694197.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9860..11311
                     /locus_tag="NK767_001191"
     CDS             9860..11311
                     /locus_tag="NK767_001191"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HCH6694198.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11332..12168
                     /locus_tag="NK767_001192"
     CDS             11332..12168
                     /locus_tag="NK767_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCH6694199.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12168..12827
                     /locus_tag="NK767_001193"
     CDS             12168..12827
                     /locus_tag="NK767_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCH6694200.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            12953..13642
                     /locus_tag="NK767_001194"
     CDS             12953..13642
                     /locus_tag="NK767_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694201.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13648..14604
                     /locus_tag="NK767_001195"
     CDS             13648..14604
                     /locus_tag="NK767_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCH6694202.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14648..15160
                     /locus_tag="NK767_001196"
     CDS             14648..15160
                     /locus_tag="NK767_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6694203.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15416..15964)
                     /locus_tag="NK767_001197"
     CDS             complement(15416..15964)
                     /locus_tag="NK767_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCH6694204.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16053..17234)
                     /locus_tag="NK767_001198"
     CDS             complement(16053..17234)
                     /locus_tag="NK767_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCH6694205.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17470..18978)
                     /locus_tag="NK767_001199"
     CDS             complement(17470..18978)
                     /locus_tag="NK767_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HCH6694206.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19137..19328
                     /locus_tag="NK767_001200"
     CDS             19137..19328
                     /locus_tag="NK767_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694207.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19531..20448
                     /locus_tag="NK767_001201"
     CDS             19531..20448
                     /locus_tag="NK767_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HCH6694208.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20441..21514
                     /locus_tag="NK767_001202"
     CDS             20441..21514
                     /locus_tag="NK767_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694209.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21662..22180
                     /locus_tag="NK767_001203"
     CDS             21662..22180
                     /locus_tag="NK767_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694210.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22161..22553
                     /locus_tag="NK767_001204"
     CDS             22161..22553
                     /locus_tag="NK767_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HCH6694211.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22629..23564
                     /locus_tag="NK767_001205"
     CDS             22629..23564
                     /locus_tag="NK767_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HCH6694212.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23697..24476
                     /locus_tag="NK767_001206"
     CDS             23697..24476
                     /locus_tag="NK767_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCH6694213.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24469..24786
                     /locus_tag="NK767_001207"
     CDS             24469..24786
                     /locus_tag="NK767_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCH6694214.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25030..26640
                     /locus_tag="NK767_001208"
     CDS             25030..26640
                     /locus_tag="NK767_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCH6694215.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26797..27645
                     /locus_tag="NK767_001209"
     CDS             26797..27645
                     /locus_tag="NK767_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCH6694216.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         8967 a         4892 c         5551 g         8266 t
ORIGIN      
        1 actgttaaaa aacttttact ttagaaaatt ggagaaattg atcgcttaag ataaattcag
       61 gacactaaat agtacaggaa gtagactaaa acttttaaaa gttttaagtc tatctatttg
      121 gcaagtttaa aaaataaaaa ttcggtatca aaaaactgtt accgataaca tttttgaaag
      181 cgttgtcttt tgcttctcta tcacttatag tgatgacgtc atattaaaac acaaggagga
      241 aatcaatgga tacagctgca atttatcatc gaccggaaag tgaatatgca tttctatata
      301 cgaatacaag atttcgtatt cggcttcgta ctaggaaaaa tgatatcaaa aaagtatatg
      361 tgctatgtgg agatccatat accataacaa ctgaaaaatg gtatcaaaaa caacgaccaa
      421 tgaaaaaatg tctaagtacc aatgttcatg attattggga aattgaagtg acgagcgaaa
      481 caagtcgctt acagtatgcg ttccatgttg ttggggaaga taacacggac tgtttttacg
      541 gtgatcaagg aatttttcct tatcgagaaa atgtgattac tgaaccaaat ttttattttc
      601 gaatacctta ttttcatcaa atcgatcgat tcactattcc agaatgggta aaagaaacag
      661 tatggtatca aatttttccc gaaagatttg caaacgggga caaagcaaat gaccccgaac
      721 acactttacc ttggggaagt aaagatccag accgagaaga ctttttcggg ggagatttgc
      781 aaggtgtgat ggatcatttg gattacttga cggaccttgg catcaatggc atctattttt
      841 gtcccatatt taaagccaca tccaatcata aatacgatac gattgattat tatgaggtgg
      901 atcccgcttt tggcgataaa aaacttttaa aaaacttgat tgatgaagca cataaaagag
      961 ggatacgtat catgttcgat gctgtattca atcatatggg tgtccattca ccacagtggc
     1021 aagacgttct caaaaacgga gaaaaatcga tctataaaga ctggttccac atccgttttt
     1081 tcccagtaga ttcttatcag atgactgact taccagaaac tgctgaaaat atcccatatg
     1141 atacttttgc ttttactcca ttcatgccga aactaaatac agcaaatcca gaagtgcaaa
     1201 aatatttgct ggatatcgca acttattgga taaaagaatt cgatattgat ggatggcgtc
     1261 ttgatgttgc taatgaagtc gatcatcatt tttggaaaaa attcagagaa gcagttacag
     1321 aaatcaaacc agacatctat atccttggag agatttggca ttcctctcaa gcttggctgc
     1381 aaggggatga attccatgca gtgatgaatt atgcatttac agattcgatt aaagattatt
     1441 ttgcaaaaaa gaaaatcaca gcttcacaaa tggtcagtgg aatgaatcat cagcaaatgc
     1501 tttacagaga tcaggtgaac gaaggaacat tcaacttatt agattctcat gatactgcca
     1561 ggatcttgac cctttgccaa ggtaacaaag aattgatgaa gtctgtgatg gcctttatgt
     1621 ttcttcaaaa agggtctcct tgtatctatt acgggacaga aatagggatg actggtgaag
     1681 atgacccgga ttgccgcaaa tgtatgatat gggagaagga ggaacaagat ctagagctat
     1741 tcggttttat aaaagaactt gtttctctaa ggaaacagtt gtctaagatt atttctgaag
     1801 gttctactca gtggctaata gtaaatgatc gtgaagataa gctctatttc acacggaaac
     1861 tagagggaca gatcatctat gtttacttta atcagtcaaa agaaccttgg gtagtagaac
     1921 aggaaaatga ggtgattctt agtcaaaatt gtcagttgtt agaagacggg aaagcagaga
     1981 tacgacaata cggatttttg atcatgtgtt gaaaactgtt accggtaaca acaagaaagc
     2041 gtttgcaaat gaagatttgt gcccattata ataagggtgt aacagcacgt tgaatacaaa
     2101 ggaggaaatg aagatgaaaa aaagaagtgt gaaacagttg ggtttagggt taatctcttt
     2161 aggaatctta ggcggattag cggcttgtgg caatggtaac tcttcagcag aaggggaagc
     2221 cgatgaagga aaaacgttaa cagtttccgt tgattctgga tataaagatt atataaacga
     2281 aataaaaaac actttcgaaa aagaaaacga tgtgaaaatc aagctagtag aaaaggacat
     2341 gttcgatcag ttggaatcat tagcgttaga tgggcctgct ggaaagggac ctgacgttat
     2401 gatggcagca tatgaccgga tcggtgcgtt aggacagcaa ggacatctgg cggaagtaaa
     2461 actgggaaat gaagcagctt acgatgaaac agacaaagcc caagtgactt atgatggaaa
     2521 aatctacgga gaacctgcag taatcgagac ccttgttttg tactataaca aagatttagt
     2581 cgatacagca ccatcaacgt ttaaagattt ggagaatcta tccaaagaca gtcgttttgc
     2641 ttttgagtca gaagcaggaa aaaatacagg atttttagct aaatggactg acttttatta
     2701 ttcctatggt ttaattgcag gttacggtgg ctatgtattt ggtgatgatg gcacaaatcc
     2761 ttcagatatc ggtttgaata atgcaggtgc tgtagaagga atctcttatg caacagattg
     2821 gttcaaaaat gtttggccaa agggaatgca agacataaaa agtgcaggag acttcgccag
     2881 ccagcaattc atgtccaata aaactgcagc aatcattgac ggtccatggc aagcgcaaac
     2941 atacaaagaa aacaatatca attatggtgt agccaaaatt cctactctaa ataatggaca
     3001 gccttatcaa ccgtttggcg gaggaaaagg atgggtagtc agcaattatg ctaaaaacaa
     3061 agacctctct caaaaatggc ttgattatgt aacgaatcag gaaaatcaag aaaaattctt
     3121 tgaaatggta aatgaaatcc ctgcaaacca acaagctagg gaaacagcaa aggctaaaaa
     3181 cgatgaatta acaacagctg tgattgaaca atatgaaaca gctcaagcga tgccaaacat
     3241 cccagaaatg ggcgaagttt ggacaggtgc agaaaacttg atgttcgatg cagcatcagg
     3301 ttcaaaaaca ccaaaagaat ccgcagatga agcagtaaaa acaatcagtg aagccataga
     3361 acaaaaatac tgattaaaat gataaccgtc catttttata aaaaatggaa caggtgcttc
     3421 agataaaagt ggcaagagcg agatggaaaa ttatcttgct cttgttgcca ttatcttaaa
     3481 agcagtttgc ggtttagcaa gtttagaggg ggcattcaga tgcagcaaga taaaacaagc
     3541 aatatcaacg aaaaaaaaat cacgctttgt tcacttattc cagggatggg gcagtttgta
     3601 aatggccaag cttttaaagg tatcgttttt ttcgccatat tcgtcctatt cattggtcag
     3661 atgcttctgg gcggatttca tgcattggta ggattggtta ctttgggaag tataccaatg
     3721 gaagatcatt cattatttat tttgattgaa ggcactttgc aattcatcat tacaatcatt
     3781 ttcttgattt tttacggttt gaatctttta gacgcttatc gagtagcgaa gatgcgtcaa
     3841 gagggcaaaa aaatcaatcg gacgatgaag gaagtcatac ataatatagg tgatgaaggt
     3901 tttccttatc tattgacact tcccgcttat cttctcatga tttttacgat cattttccca
     3961 gtcctagtta cattgttcac tgcatttact aattatgact tcaatcatat tccaccagcc
     4021 agtttaattg attgggtagg ggctaaaaac ttttttagta tcttcttttt aagctcatat
     4081 cgtaatactt tttttgcagt cttttcgtgg accgtgattt ggactttatg tgcaacaact
     4141 ttgcaaatca cccttggtat tctcacagcg gttgttgctc accagaaatt tattaaattc
     4201 aaacgtattt tcggtatcat tttcttatta ccatgggcgg tcccagcttt tattactatc
     4261 atgagttttt cgaatatatt caacgatagc gtaggtgcca tcaatactca agtgattcct
     4321 ttcataaacc agcttccttt tgtagatatc agtgcaatct cttggaaaac ggacccgaac
     4381 tggacgaaag tagcaattat cttagtgcag ggctggttag gatttcccta tatctatgtg
     4441 atggttacag gaatcttaca atcgatttca gaagacttat atgaagcagc aaaaatcgac
     4501 ggagcaaatg catggcagcg cttttcaaac atcacgcttc ctgctgtctt cctagtggca
     4561 gcgccaacat ttattacaca gtatacgggg aatttcaata atttctccat gatctatctg
     4621 tttaacaatg gcggtcccgg aagcgtaggc ggaggagctg gttctactga tattcttatt
     4681 tcctggatct ataaacttac gactggaact tctccgcagt attctatggc ttcagcaatc
     4741 acgttgatta tctccgtcat cgttatctgt gtgtcattgc tggtatttaa aaagacaaat
     4801 gcgttcaata tgggggatta acaatgaaaa aatttcattc gagtgctcga ttccaaaaac
     4861 gactttctca cttttttact tacttttttc ttttaatact gtcagtcatt atcatctatc
     4921 cgcttttgat cacagcaagt actgccttca agtcaggaaa tattgcagct tttagtttag
     4981 atctttcaag tgactggact ttaggaaact ttactcggct attcagtgaa acactttacg
     5041 ggacttggta taaaaataca ttgatcattt ctgtcctaac aatgatcgta caagtgactg
     5101 tagtaacttt ggcgggctat acttatagtc gctatcgttt tatcggccgc aaatcaagtt
     5161 tgatgttctt tttagtgatt cagatggtac cgactatggc agcattgact gccttttatg
     5221 tcatggcttt cttattagaa gcgctcgata agtactggtt cttgacaatg atctatatag
     5281 gcggtggaat tccaatgaat acttggctga tgaaaggata ctttgacact gtaccaatag
     5341 atttagatga atctgctaaa cttgatgggg cagggcattt caggattttt ttccagattg
     5401 tattaccgct tgtcaaaccg atgctcgccg ttcaagcatt atgggctttt atgggacctt
     5461 ttggggattt catgttagct aagttcctgc ttcgaacacc tgaaaatcag actgttgctg
     5521 tcggcttaca aacatttatt tctgatgtga caaaccaaag agtctctctt tttgctgctg
     5581 gggcaatttt gattgcacta ccgatttgtc tattattctt ctttttacaa aagaactttg
     5641 ttaccggcct gacatctggc ggaacaaaag gataggagca gagtatcatg caaacatttc
     5701 cgttaaacta tttttccgca ttacgtacgc caactcaagt gtttgcggga cgcaaactat
     5761 tatcatggcc taaatttttc ttgatatttg tatttttagt ttccttgatg gtcatgcctg
     5821 tgactctttt ttatgccaat cagatccaag cgattccatt agaacaattt ctatccgttc
     5881 attcgctgat cgatgaacaa ggaacccaaa aattttctga acttgaatta tctgaaactg
     5941 ggttacaagc atcgcaacaa acgatagctg tgacccctga aattttggta ggtgtgtctt
     6001 tatctgagaa acaacaatcc gatcatggaa cttttattga ttttgagaag gaacaatggg
     6061 tgatccaaca aaaggataaa tctggtatac gccgctatac gatgaattat agcccatctt
     6121 tccaaccaga ttctgtacgt acgcccgaag atttccaacg atttttagaa agagaatttt
     6181 atgccagtaa tcgtccaaca atcattttat cttatagttt aagtcttggt cttgtcctat
     6241 ttgtcatgac gagtctcatt ttattcgggg cttccttttt cttgtggatg acaagaaaaa
     6301 gccaactttc atccattcat acattcaaag agagtgcaaa tttgatgctg aatgttatgg
     6361 gaataggttc catggcagca gcagttgtag ggtttatcca ctttgacttt atcttgatgc
     6421 taagtgtcca aacaacgata actgtcttgc tgcttttatg ggtttttgtc aaaacaagat
     6481 tcaaagataa gcgtgtcgaa tgacaagttt aagcgattta tgcgacaatg aatgtgaaaa
     6541 aaatgaaaaa taaagtagtt tttgaaaaat ttctgaaaaa attaaacttc ttagttgatt
     6601 cttttctttt ttagtattaa cgtaataggg tagttttttt aaggaggaaa atcatgttag
     6661 agaaaacttt atacaatcaa ttattttcac gttctttctc gctccctgtc gaagtaaatt
     6721 attgggacgg tacaacgaaa caatacggag atacggacac tcctccccaa atcaaaatca
     6781 atatccatga agaaatccca atgaaagaga ttacaagtaa cgcttctttg gcactaggag
     6841 aagcctatat ggatcatcga atcgagatcg agggaagtat ccaagcattg atcaacgatg
     6901 cttatcagca agcaaacagc tttttacgag gaaaagatta tctaaaatgg ctgccaaaga
     6961 aagaaaaaca tacgaaacaa aaaaacaaag aagatatcca tgcgcattat gacttaggta
     7021 acgactttta taaactttgg ctagatccaa ctttgactta ttcttgtgct tatttcaata
     7081 cacctgaaga tacgttggaa caagcacaaa tcaataaagt ccatcatatc ttagacaaac
     7141 tctttatcaa agaaggcgaa acgttgcttg atatcggttg tggctgggga acattgatgt
     7201 tcactgctac caaagagtat aatgtcaagg caactgggat cacactaagt gaagaacaat
     7261 acgatttgat taccaaaaga ataaaagaag aacatttaga agataaatgc cgtgtattat
     7321 taatggatta ccgcgaacta aaaggcgaaa cgtttgatca tatcacaagt gtaggtatgt
     7381 tcgaacatgt aggttctgaa aatcttgaag gttatttcaa agtggtaaaa gatttattga
     7441 aaccaaaagg aacagctttg atccatggaa tcagccgaca acaaggcggt gcgacaaatg
     7501 cttggatcaa taaatatatt ttccctggcg gttatgtccc tggtgttact gaattagttg
     7561 ataatattac aaaaaacgat ttgcaactga ttgatttgga aagcttacgt cgtgactacc
     7621 aattgacatt agaacattgg acaaagaatt ttcatcaagt ccaagatcaa gtcacgaaag
     7681 aaatgggtga ttccttctat cgaatgtggg atctatattt acaagcttgt gcagcttcat
     7741 ttgaatcaag taatatcgat gtgattcaat atttgcttgt tcaacctgga aacaatgaca
     7801 tcccaatgca tcgtgcataa ttgaggcaaa agtactgttt aaagactatt taaaaagaag
     7861 ctaagtttta tgatgctcca gaagtgaact tttttgtcta acttctagga ttgtctctaa
     7921 agcttagttt ctttttcttt atattttcat aataaaagtt tggttcttta atttttaaac
     7981 atgacagaaa attttttctt cgttatactt gtaatagaat actattttat aacaaagggg
     8041 atcgaatcaa tgaaaaaaac tgttatttat actagtttat ttgcagcttt gtttttagct
     8101 gcttgtcaat caaataccaa tcaaggcaca tctgggaata cgtcttccag tagcgaacag
     8161 cgctcttcac aggtatcgga ctcctcaaca aaagcaagca catcttcgtc tagccagaca
     8221 aaaagttcta gtgaagcaag tactcagtca gatatcaaag tgtctgcagc tcgtgcgatc
     8281 gaattgttcc aagataaata cccagatact gcaattacta gtttggaatt agatagtgat
     8341 tggggatctt acttctacaa gatagaaggt gtcgatgatc aaaaagaata cgaagcaaga
     8401 attgatgctc aaaatgaaaa aatggagaca gagaatccag aaactctaga tcgagatgaa
     8461 caaaatggtc aaaagaaaaa agaagatggt ctggatgttg ataaattgat cacgattgag
     8521 gaagcagcaa aaatcgccgt cgacaaagta ggaagcggta cggcaacaga ttgggatttg
     8581 gataaagatt taggtactac ttattgggaa gtcaaagtaa aagacggcaa tcaaagtaca
     8641 tcagtcaaga tcaatagcca aacaggcgaa gtactgtcaa ctgaacaaga tgattaagaa
     8701 tttcttcgga aaacaagagt tgatttttct tttagtcatg atacactaga caaaataact
     8761 tgtttgaagg agcgatttac ttgagtttag aaacgacctt tgccaatcat atctttgcca
     8821 atcctttgat gaatgcatct ggtgtccact gcatgactac ccaggaatta gatgaactgg
     8881 ctcattcaga agcaggagca tttatcacta agagctgtac gatcaatgaa cgaaaaggaa
     8941 atcccgaacc acgctatttt gatgttcctt tagggagtat caattctatg ggacttccaa
     9001 acttaggttt ttcttattat ctagagtatg ctcttgcgta tgaaaaagtg caggaaaacc
     9061 aaaatcaacc gttatttttt tccattgccg ggatgagtgt ccaggaaaac ttggaaatgc
     9121 ttgagaaaat agaaaaaagt gggtttaatg ggatcactga attaaattta tcttgtccca
     9181 atgtgcctgg aaaaccacaa ctagcttacg attttgaggc cacttatgaa acactgaaag
     9241 aagtattcag catcttttct aagccgctag gaatcaaatt gcctccttat tttgattttg
     9301 ctcattttga tcagatggct gatatcttga atcagtttcc attgacctat gtcaatgcta
     9361 tcaacagtgt gggcaatggt ttgtatatcg atacagaaca agaagcagta gtcatcaaac
     9421 caaaagaagg gttcggcggc attggcggag agtatatcaa gccgactgct ttggcgaatg
     9481 ttcgcgcttt ttatacacgt ttgaaaccag aaatccaaat cattggtaca ggtgggattc
     9541 ggacaggaca agatgcattt gaacatctgc tatgtggcgc aagcatgtta cagattggta
     9601 ccgagcttca taaagaaggg ccggaaattt tttctagaat cataaaagaa ttaacgcaga
     9661 ttatgtcaga aaaaggatat acgtcaattg acgaatttaa aggcaaatta cgcaccattt
     9721 catagcaaag agacgaaggt catgattgat cttcgtcttt ttttataaaa aacactcgtc
     9781 tgttcattcc tgtgcaagcg tactcattgt atgttagacg aacttgtggg aaaataacag
     9841 aggcaaaaag gaggataata tgaatgatgc aacagaatta gcaaaagagc ttaaagaaaa
     9901 agaatttagt tttcaagaac tagttacgga tatacagaaa aaaataaaga agcaaaatca
     9961 tgaattgaat gcgtttgtcg cgcttgaacc attcgagagt aattcccacg agctattgat
    10021 tccccagtca gaaaaagggc cattggctgg tatcccattt cctttaaaaa tgctcggtca
    10081 agaaaaaaag ggatggctag ctacttcagg atctcgttta tttgaaacac accgagcttc
    10141 tcatacatct aattatgtgc ggcaagcaga agcgataggt ctagttcctt ttggacaaac
    10201 caatgcgcca gagtttggtt tcaaaaatat tacagaccca gtcatctatg gcccagcaag
    10261 aaacccttgg aaccttgaat gtacaccggg cggatcaagt ggaggagctg ctgctgcagt
    10321 ttcatcagga atcgttcctt tagctggtgc tagtgatggc ggtggttcta ttagaatacc
    10381 cgcttcgttt tgcggattga ttgggctgaa accttcgcga gggacgatgc cagttggtcc
    10441 atatgcttgg agaggatggc aaggagctgc tattgatttc ggcttgacag tttctatgag
    10501 agacacggag gctttatttt atgggatgag aagtatccat tccggtgcgc catatcaagt
    10561 atcgccagtt gagtggcaaa cacatccaag aaaaaaacgg ttgaagatag ctttttgtgt
    10621 tgattcaccg atccactcac agattagtga agaggcagta cttgctgtaa aaaaagctgt
    10681 agatttcctt ggaaaagaag ggcatgattt gacagagatt ccttacccac tagatggtcg
    10741 taaactgatc acgtcttatt atcagatgaa tggcgcagaa acagcagcaa tgatccgctc
    10801 gattgaaaca ggcatccagc gagctgtcag aaaagaagaa cttgaaccca tttcttggac
    10861 tttgttccaa tatggtcaga agctctctgc cgctacttac atcgattctc tgcatgtatg
    10921 ggatgaagca gcaattgtca tggaaaacct acatcaagaa tacgatctat tcctttcacc
    10981 aacgactgca tttaccgccc ctaaaatcag cgaagatttg caaagtgatg cgataagagc
    11041 acagatggaa cacatcagtg attgttctga agtagaaggt ttagacgttg tttcttccat
    11101 gtttgaggag agcttgaaga tcacacctta tacacagttg gctaatctga ctggacaacc
    11161 agcaatcagc ttgcctactc atttgacaaa agaaaacatg cctctaggaa tccaatttat
    11221 ggctgccaga ggaagagaag atttgctatt tcaaatcggc tatctatttg aagcaaatca
    11281 tcagttccat ttaccaaatc catggaaata gcagaaacaa ggagaagaag aatgaagaaa
    11341 aaaagattgt ttgctctaat aactagtgcc ttttttattt taagcgcttg cggtacagct
    11401 gccgctaatc aggaagataa cagctggagt aatgtagaaa aaaagaaaga attagtagta
    11461 gcgacttctg gcacactgtt tcccgcatct tattataatg atcaaaatga attagttggt
    11521 tatgatgtag atttggcaaa agaggtggct aaacggttaa atgtcaaaat ctcctttaaa
    11581 gaatacaacg tggatggaca aatcacttct gtttcaaaaa gagaatcaga ttttgctgcg
    11641 aacgattttg gtatcacaaa agaaagagaa aaaaaattct ccctttcaga gactttcaaa
    11701 tattcatttg acagtatgat cgtacgaaaa aaagatgatt caggaatcca ctcacttgaa
    11761 gatcttaaag ggaaaaaagc agctggcgaa cccaatacta gctatatgcg tttagcggaa
    11821 aaacacggag ctaaattagt cacatatgac aatgccacga atgatcagta tttaagcgat
    11881 gtagcaaatg gtcgaacaga tgttattcta aatgattatt atctgcagaa aatggccgta
    11941 tctgctttac cagatatccc tgtaaaaata ctggagaacg tttatttcaa tgaaaacgaa
    12001 agtggttttt tattgaaaga aggaaatgat gctctgaaaa agaaaatcga tggcgtatta
    12061 aaagagatga aagaagatgg gacgacaaag aaaatatctg aaaaatattt tcaaactgat
    12121 gtttcagtca aaccgaaaga aacgatggct gagtcggtga gtgagtgatg tactttcctc
    12181 taattgattt ttccctcatg tggacttcac ttcctttcgt actgcaaggc ttggtataca
    12241 cattagggat tggcttcgtg tcgtttcttt taggaaatct ggtagggttg cttttgacag
    12301 ttttaggtct tttggattgg ctgcctctga atgtatttat tcgtttttat ttatcgttct
    12361 ttagaggcat tcccgcacta gtcctgctgt ttctgttata ttttgggtta ccttatcaat
    12421 taagtgcctt aacggcttca gtcatctgct ttacgattac cagcagtgca tttattggtg
    12481 aaatctatcg aggatcacta gcaggcgttt cttcaggtca gtgggacgct ggtttagctt
    12541 ctggtttttc tttctttggg gtattgaaat atatcgtttt gccgcaagct tttcgaatct
    12601 cagtaccggc actcagtaac gtagctatgg atctagtgaa aggtacttcg ttagcagcaa
    12661 caatcacagt gccagaaata tttcaaaaag caaagatcat aggcggtcgg acattcgatt
    12721 atttttctct ctatatttta gttgcactga tttattgggt catttgtatc gggatcgaaa
    12781 aagtgcaaca gtatttagaa aaacgttcta tgcatcagtc aatctgaaaa atagaaatgg
    12841 tgctagcgtg gctggcacca tttctatttt ttgtttcttg tatattctgc ctagtatttt
    12901 aaaaccattt catgtaaaat gtaaatcgaa aatatgttcg gaggtagaaa acatgaagac
    12961 cccgctaacc atcgaaatag aatcctcgtt ataccaatat tgcatcgagc aaggcggctt
    13021 ggttgtagaa gaagtcacga tgcctgatga ccaaggaatt gtagatacgc ttagctgctt
    13081 gacaaaattt gatggaagcc gagaatggcg atgctatgag ctgaaagtga caaaagcaga
    13141 ttttcgctct acagccaaac tgtcatttgt tggacattat aattattttg ttttaccaag
    13201 taaactatat gaagaagtaa aaactgaaat ccctacagga ataggtgttt tagtgtatcg
    13261 tccgtacacg gttgaagagg agatgcctgc ctctggtaca tttctgattg cgaaaaaagc
    13321 agcaaagaaa gagcttgctg taccggaaga agctttgaca aaccgcttca tggcttcttt
    13381 attcagggaa gtaagaaaag caaagcaaat ggagcgaggc gtaaaatatt ttccaacaga
    13441 tcaattgtac aaagaattga aaagacgttc agaaaatcaa gatccatttt ctaacgaacg
    13501 atattatgaa cggtttttag aggaaatagc tagtcaggca atcaatgagt tgcaagaaga
    13561 agttatcgcg cttcagcatg attatgaata tttgaggcaa caacagcaga tccgtcggtt
    13621 gcctacagag ccattggagt gaaaaatatg tactatcctt atttaagagg aaaacaattt
    13681 gacctattag cattaaaaga agcactgtcg cgaggattgc tctccaataa aatccaacca
    13741 gtgatcgaac cagtacgtga ttccgctacg cttaaaaatg tgatcgaatt atttcaaaaa
    13801 aagcaccatc caatcgctgt gatccaaaat ccccaagtag gacaattcaa attattcgat
    13861 cagcatgtgc acagttggac agtcaaagaa aatagctctg tcgtccctgc acagatctta
    13921 acgccagaaa atctcagtga agtactggat tcacctccag cgtttcttgt tttcgatgga
    13981 cagcactacc caaaagatac agaggtgtgg aaacagttgg caggagttga ttcgaaattt
    14041 ttgataccag atactagtcg ctttcgcatc tggctgcccg aaaacaaaat tgtgatccgt
    14101 gactcttttc aaacgagaaa gcatgttgaa agttatgcag ataaaacaga tgactttttt
    14161 agtgatgact atcttttttt ccacgcagac ggatatattg gattttctga tttcacgatc
    14221 gaaggtagcc gttatttcga taaagggttc cctagccgag cgcttgctat ccatctaacg
    14281 tatattgatg cttacggcaa tatacgcgtg aaacattttg tctctgacag taacgactct
    14341 gctaaagacc aagcattgaa gtttcttgaa gcaggagata aaatgaaaaa atggatcatg
    14401 cgtcaccatc atcaactctt gattacttca ggaatgattg aattattggc tctttaccag
    14461 caacaaaaat tccctggact gggagtgttg aaaaagtggt cacttatgca tcatttggaa
    14521 cttattagtc acctgctaga tcatccaaaa gattggctta aatattattc aaaagttcag
    14581 gaactatatg aagtgatcca atgattagat aatgtaaatg aaaaaggaat agaaaagagg
    14641 aaactggatg tatattcggc aaacaaggaa agaagaagta gaagaagtgg tagaaatcat
    14701 taatgccgca gcggatttca ttgcttcgca aggctctcct caatggcaaa acgggcagaa
    14761 accaacaaaa gaaacgattt tgaacgatat tgaaagaaaa gaaagctatg tattaatagt
    14821 ggaagatcaa attgctggaa cagctgcatt agtctcaagc gtggatcccg tttacacaaa
    14881 aatagatggt agttggaaag aatcaactgg tccttattta tcgattcatc gtgtcgcttt
    14941 agcagaaagt actcgaggaa aaaaactagc aaaaccgttc ttagaacatt tgttgacgat
    15001 tgcagccagc aaaaatatcc aagatatccg tatcgatact tatccagcta atgaaccgat
    15061 ggaaaagacc atctattctg ctggattcac ttatcaaggg atgattgaat ttccttttga
    15121 acacggcgaa agaaaagcat atcaaataat catcaattga ctataaaatc atctgagcaa
    15181 agataaagac attaacacta ctgaaaaaaa caatcatatg atagccaaga ggttgtaaca
    15241 gaagtggcca gcttcaaaaa acaagaaaga atttccgaaa atgcttctca aatttttgtg
    15301 gaattttagc gtattttacg aaatagctgc tttctgaaac tcccttcatc gagaggtcgt
    15361 gacatagctt ttgtcacgac ctctcggctt atcctacgag tattattcga tacatttata
    15421 tcaataatcc tttttttatt ttatctgcag cttcttggaa cgctccatcg tttgacacga
    15481 ctaaaagtga ggggatagct ttcaaacgtt tcaactgtgc taaatcttct acataaaccg
    15541 ccagtctggc tgaaatctgt tcagatgaat ctccacgttt tttcaggcgc gattgcaatg
    15601 tttctttaga gatcgtaata aagataggac ggactaccgt cccgaaatac tcatataaat
    15661 gccaaaaacc atttgctgtc aaaacggcgt aacaatttcc tactgtcagt ttctttttaa
    15721 tttctgcttt actggaacca tatttatgtt gagcatattg gtcccactct gcaaactcat
    15781 ttcccttgat catctgattg aatgtattgt cagaaacaaa ataataatcc tcttgatctt
    15841 tttccccagg tcttggtgat cgattcgtgt aagtaatgat cttttgttcc ggctgaaaaa
    15901 tagcttctgc gagtgaagtt tttccagaac cagaaggacc gataaataca tagcaataat
    15961 tcattgtcat ttctcctttg atgattgaac aagtgaaaaa gctttggaaa atatttccaa
    16021 agcttttaaa agtaactttg cttttttgta tattatacca gcaagccagt cacaatagca
    16081 cttaaaaagc tgactaaagt cgctgttagc aaaattttca atccgtgttt tgcgacaaga
    16141 tttccttttt cttcatttaa tcccttcatc gcaccagaaa tgatcccgat agaagagaag
    16201 ttggcaaatg aaacgaggaa gacagatata attgctgttg tacgctcagt aaaatggaaa
    16261 ttaccatttg ctaattctgt catagcaaca aattcatttg taaccagttt agtcgccatg
    16321 atactaccgg catccactgc ttctttccat gggattccgc tgatgaaggc aagaggggca
    16381 aagatgtaac ctaggatctc ttggaaagtg atgccgaata tcccgtggaa gatagcattg
    16441 atcatggcaa tcaaagcaac aaaaccgata agcatagcag caaccgtgat cgccacatgg
    16501 aaaccatcaa gaatgtattc tcccaacatt tggaaaaatg tctgttcttt gttttcttca
    16561 atgatcagct catcttcttt ttcttctaat acatagggat ttacaattga agccagaata
    16621 taaccgccaa ataagttcaa aacaagggca gtgatcacat atttagaatc gatcagtgcc
    16681 atataagatc cgacgatcga catagaaact gtagacatag ccgatacact catggtgaac
    16741 aaacgtttct cagataggaa gggtaattcc ttttttatgg aaataaaaac ttccgattgt
    16801 cctaaaatag cagaagctac tccattataa gattccaatt tgcccatccc gttgatttta
    16861 cttaatccta gaccaacagc tttcataaat aatggaagaa tcttgatata gcgtagaata
    16921 ccgataatag cagaaataaa tacaattggc atcaatacgg aaagaaagaa aggtgcactt
    16981 cctttatttg ctacaccggc aaaaacaaag tcaacacctt gcccagcaaa agcaagaaga
    17041 tgatcaaaca tttttgccaa tacagaaaca accgctgtac caaaagttgt acgtaataat
    17101 acgaaaccaa gaacaagctg taaaacgaac atcacaatta gtggtttcaa tttgattgct
    17161 tttttgttac tgctgatcaa aaaagcaagc cctaatgtga caaataaacc aataatacct
    17221 aataaataac tcaaataatc actcctattc caaattccgt tttattttac ctgttttgca
    17281 aataaaatac taatctttag tgaaattttc attacttttt tagagtgaaa cgcattcatt
    17341 ttccattaga aaataaagcg aacaaaataa aaggtttaaa aagtgtaacg tcttagtaaa
    17401 gtctataaaa aaacgaaact atcgattaag ctaatagttt cgttttcaac atttataagt
    17461 aaactttttc tattggtaat atagattttc ggaagggtag acaggtgcgc aggtaatatc
    17521 gattcctagt tttttcaatg tttgttcatc atttttattg accatcacag tagagtgggc
    17581 ttgtgctccc tcaagttcag ctagtttatc ataagcaact tgagcgattg gattcgttac
    17641 tgcactgatt gataaagcga tcaagacttc attgatgctt agtgcatcca aagaggaatg
    17701 aagtgtcttt gatttcaatc cttgtattgt ttcaagaact aatggtgaca ataaaggaat
    17761 gtcatctgcg atatgggcta aatgttttac tgcatttaag ataactgccg ctgacgcatc
    17821 catcaatgaa cttgtacgcc cggtaatgat gacttcatct ggtaattcga ttgcaatcac
    17881 agcagaagga tcttttgtag gactttgcgc ttgaatctta tctgcatagt catgggcagg
    17941 gactaccacg tttcggtctt caggacgtaa accgacttct tccataatca gtttgattcg
    18001 attaactgct tcttcgtcga tcaatccttt tttgaagtcg cattctgttt ggaaataacg
    18061 acggatgatt tcttgtttag aggcttcttg gacaacggta tcatccacga tcccaaatcc
    18121 tacacgattc acacccatgt cagtaggaga ttgataaaca gattctttac cagtaatttt
    18181 ttcaataata cgtttgatta caggaaatgt ctcaacatcc cgattatagt tcacagctac
    18241 tttattgtag gcatcaaagt ggaaggaatc gatcatattg acatctttta gatcgactgt
    18301 ggctgcttca taagcaatgt tcagcggatg tttcaacgga acattccata ctgggaaagt
    18361 ttcgaacttg gagtaaccag ctgcatgtcc tttttggctt tcatgataca gttggttcaa
    18421 ggaagtagcg agctttccac ttcctgggcc aggagctgtc actacaacga ttggtttagt
    18481 tgtttcaata tactcatttt ttccaaaacc atcttctgag acgatttttt ctacgttgac
    18541 tggatagtct tcgatttctg agtgtttata gaccttgatg tttcgtcttt ctagtttatt
    18601 gataaagact ttcgtggcag gctgtccact atagcgagtg ataacgacac tgttgattaa
    18661 taagccataa ccacgtaatt catcgattaa ccgcaagata tccatatcat aagtaatccc
    18721 ataatcacca cggatcttat tacgttcaat atctccagca tagacgcaaa tcagtatttc
    18781 agcttgatct ttcaattttt gcagcaattt gattttcgca tcttcttcaa atcctggaag
    18841 tacacgtttc gcatgcttgt cgtcaactag cttcccacca aattctaaat ataatttgtc
    18901 atagtggttc actcgttcca aaatatacgc tgactgctct tcaatatatt tttctgaatc
    18961 aaaaccaatt tttttcacta ggttccctcc ttaagtacta aagcattttt tcattttttt
    19021 gaggattcgt caactggaag agcgaaattc ctcaaaaaaa caaagaaaat ggtttaaacc
    19081 aacttgtcta gatagttatt cattggtatg atagtttttg ataaggaagg tgctacatga
    19141 aaaaaagaca gaaaaagaaa aacgcatata aacactatat ccgttcgatt tttaccggtt
    19201 atgaaagaat gcttgaagat cccgaattgg aacaattgac gtttacttat ctaaacgaag
    19261 aaacgcagct aactcgtgat gagcatcagc gcattcattt cacaacaaga gatctgccta
    19321 gtaaataaaa aataagataa aggtcatcgg attcgattct gtcctatcag atggaaagac
    19381 tagttgatcg aaaaaaaggc tgtaccaaat tttggtgcag cctgttttat ggagcattgc
    19441 ccgctctttt ttataacaat tttttacaga aaatggtact attgaagtat taataagtga
    19501 aaaacagatg agtaagagga ggcaagtagt atgaaagagc cagatacaaa tgcaaattat
    19561 gagccagacg aactagagga gacgaatgcg gatcaggata caggacaaga atcgtcttct
    19621 ttttacgata tcaaagaaaa agcaagcatg aatctttccc aattattaga agacttacag
    19681 gcttttgaaa aagctgtccg cgaagaagat atccctgaga tctaccgttt atacaatggc
    19741 cgattgaatg aagaattaaa aagaagctca aatgaaaatc acgagatcga tcaattgctg
    19801 atgcgtaaga tccatgatcg ttttttacag gaatttcctt ttatggaaca agtagaaaac
    19861 atctcgccaa cgatgagcta ttacaaaatc ggcacttatt accatgatcg gcccacggtc
    19921 ggtattgatg caagtctgcc agaaattttt gttttgccta agatcgatga agagtgggag
    19981 aaatattccc aaccggagtc aacggactat gacaaaaaaa tcaatgagct agacgcaaag
    20041 gtgatcactg ctcagacaga aattgaacgt ttagatgaac aaataaaaga aatcaatcga
    20101 caaatgactg atttgaacga caataaagga ttcttgaatc gtaaaaaaat cgaggaagaa
    20161 attcaagaat tagagaagaa gaagcaagta ctatctaatg aaaaattagg ctggcttcct
    20221 tatattgaga cgcctgaaac aatccagcag caaaaagaag cattgaagca agaagcaaga
    20281 gcagaccagc ttcgtgcagc catcgtagaa aaagaacaaa ggcaaatcag acgctatttc
    20341 ggtagcaaag agggattcgg tcaagcaatc catgaattct tgatgaatta tcttggcaat
    20401 gagaatccta ataatcaatc taatgaagga ggttccgagt atgaatgaac acgatgaagc
    20461 agaatggaca aaaaatgaag aacaaacaga agatcatgaa gaaacgaaca cttcattgga
    20521 agaacaaaca agtaatcctc tggatgacac agaagaaatc cctatgctag aggaaacaaa
    20581 aatacacgaa aaagaaaaag taactccgac gaatacgaat actgcagaaa acacccatgc
    20641 ttacgaaata actgaaaatc ccgggtattt agaaggaaaa gagcaagatc atcaagatat
    20701 cgtcagtttg attgaaacag cggaagcaga attagcggat ttacgtatga gaaaagattt
    20761 gatcgaatca gattttccac aattattaga atcatatgat cgttttatat tgaatgaaga
    20821 accaatctcc acgatcggaa agacaagcct gatcgaatac ttcacgtatg aattactgaa
    20881 accgttgaat gatattggtc tggaacggtc aaattcagct tatgacacat ggaaaacaag
    20941 acatttttcg atcaattata cgttgaatgg acaaaacgaa ttagtctttt cctttgtatt
    21001 accgaccatc gatcaacgat accaagtgga atcgatgagt ctcctaaacg tatcaccaga
    21061 gacaatggaa atcaatgtcc aagatgaccg ggtgctttct ttgatccgac actggtcagt
    21121 ggatcgagta ttctctgcag ggcagatcac gatattcaat cacaaattga atcaattatt
    21181 agcccatgca agaacacttg gttttaccgt taatcagaca ttactagaca atacgaagcc
    21241 acttcgtttg aatcttcaga gcgaatttga attgacgaat gaagtattgg atgatatttt
    21301 tatcgtggca atgaaaaatc catcctacga tatggaaaag atcaacgaaa ctacttatca
    21361 agtacgatta gataaaggac agtctttaac gataacaaca aacgaaaaag atcaaacagt
    21421 cttgtcgatc tcatctggag actacccacg atctgtcatt gatttcttta tcaattacga
    21481 attcttggtc ccattaatgg tgagaaagtc ctgattggat aagttggatt accagatgtc
    21541 attcagataa ttctgttcca aaggacttgc ggcagttatc cagaataatt gtcacaagtc
    21601 ttttctgtat gtactaaatg gacgtgaata acttgctgat gaaggaggga tttcattagg
    21661 aatgtccaca aataaaatag cacagcttag cttattgtct gctgcttgtg tagcaggtag
    21721 gatcgctttt caatttattc cgaatttcca gccaatgact gctatttttt tatttatagt
    21781 actttactta agcttaaaag atgcgttaat tgtcatgagt ctttctattg cgatcagcag
    21841 tttttatttt ggtgttggac catgggtcat cggccaatgg atcagctata ctgttgttct
    21901 gagtttgttt tcaatcgtct gcttgagaaa aacagtgcaa aggaatctat ggttaaaggg
    21961 agtctgtttt tttctggcgg gcatggttta cggaataggt atgaccgttt ttgatacgct
    22021 gttatatcgt ttgcctcaac cttggatcta ttaccttcaa ggggtgtcgt ttgatttgat
    22081 gcacagtata ggcaatgttg tgttctttct tgtttttctg ccagtcgtca aacgttttta
    22141 caatcattca ggaggaaaaa atgaaaaaaa taatttatag tttgcttgca atgagtgtca
    22201 gtatactcat tttgacaagt tgcagtacaa cctatacaca atcggcttcc aaccaaaagg
    22261 aggaagtaaa aacatcgcaa aaagcaacaa tcacgttaca agaaaatggt caaaactttt
    22321 cggaaaaaga agtgttcttc aaaaaaggcg atgatttact gagtattttg aaacgaaatt
    22381 ttgaagtcaa agaagataac ggcttcatca cttcgcttga agggcactca caggatgaga
    22441 aaaatcaact ctattggatg tttactgtgg acgggaaaag tgctactact ggtgccaaag
    22501 agatcaaatt gaatgatgga caatcggtca ttttcaattt gtctaaacta taagtttcat
    22561 aaaaaactgt taaagggcga atattgccat aattcagtga gttcagttga tgaaagaagg
    22621 aaaataacat ggaaaatttt aaaaaaagcg gaatgttttc cgtaattgct gctctagggg
    22681 ctaatattct agttgctatc agcaaatttg tcggctatgc aatcagtggc agtgccgcca
    22741 tgctaaacga aagcatccac agtatcgttg attgcagcaa tcagattttg ctgttattcg
    22801 gtgataaaca agccaataaa ggacagagcg agttgcatca atttggtgaa ggacgagcaa
    22861 aatacttttt cagtacaatc gtcgcaatga tgcttttctt cggcggtggg gcacttgggg
    22921 tgatggaagc ttttgaaaag ttgacgcatc catcccatga agtgggtaat ccgatcattg
    22981 ttatagcaat actgttattt ggtttggtaa tcgaaggaag ttcgttacgc gtggcaatga
    23041 aagaaatcaa agaattgaat actgaaaaac tttccacgat gcgctttttg cgcgaaagtc
    23101 gacacagtga aatactgatt atttttacgg aagacttgtg tgctgtaatc ggtttggtct
    23161 tggctttagc aggaacaata ttgacttatg caaccgggat tgctgcattt gatgcgatca
    23221 gcggattgct gatcggtttc ttgctgatgg gagccgccat tttcctagca aaagaatttt
    23281 atagtctgat catcggtgaa agcgtgacag caagcgattt atcaaaaatc aagttagctt
    23341 ttgaacgtcc tgagatcagt cggctgatcg atataaaaac agttcattta agtccaacgg
    23401 agattttagt tgcagcaaaa gcagatatcg ttggtaacaa agagaatgaa gcttactcgg
    23461 tcatcaatga tattgaaaaa aatatgcgtc agtctcttcc tgacaagaag atgtatatat
    23521 acattgaaac agataaatat gacccaaatt attctcgtaa ataaaacgtt tattcatgaa
    23581 ttgaagcaaa aatgagaaaa atataatctt gtttttaaat aaaacggcta caatttagcg
    23641 aatttatgtt acaatgagtc cgctgtttat ttgttgtaat taaaggagat attttaatga
    23701 acaaaaagaa aagtgcgcca tggcttcaag ctttgcatgc agtgatgcca ctttgtataa
    23761 gctatatacc agtaggatta gcttgcggag tattgttgca aaaagttgga tttgatcctc
    23821 tcttatcagg actgttatcg attctggtct tttccggtgg cgcgcagttt ttagttgcat
    23881 ctatgcttac gacgcaagca tcatttgcaa cgacattatt gatggttttc tttttagaat
    23941 tacgctatac attattagga gcaagtcttg ccggttttat gaaaaaagaa aagcggacgt
    24001 ttttggctgt cttttctcaa tcactgaatg atgaaaacta tgcggtgaac tacttaaagt
    24061 tttcaactga cccttcctgg aataaaagaa aggctttata tgtcaattgg ttctcaatga
    24121 ctgcatgggc cggtagcaat atgatcggta attttttcgg ctcggtgatt cgagtagatg
    24181 ctgaccttgt ccattttgct ttgacggcca tgttcatttt catgtttatc atgcagatga
    24241 aaagcgcact actgatcttt acaggtttat tctcaggcgt attaggggtc gtattcatgg
    24301 tattattcca aaatactttc ggattgattg cagcaacagt cattgcttca tttgccggat
    24361 ttatgctgga gaaggcattc aaaaaagaaa aagctaagaa actaagagac cgcggtaaag
    24421 atgtcaatga gccgctcttc aacttcccgg aacaggaggc caatcaccat gactaaacta
    24481 tatttgctac ttttagttgc ttgtctgttc atcgtggcat atatcccgcg cttgttcccg
    24541 atgctttatt ttactcatcg aaaagtgcca tcctggttca gtgactggat gaaatatgta
    24601 ccggtggcat tatttgcggc gttagctttt aaagatgttt tcattactca tgaacatctg
    24661 gatattgctt ggaatatcaa aatagctgca atgattctag tagcaggcgt ggcttacaaa
    24721 acacgttcga tggcgttgtc agttttgaca ggacttgcgt ctgtcttttt gctgtctatg
    24781 ctttaaactg ttttatttta ttaaaaacag agattgcgac ataagttgtt caaactgatc
    24841 tatccgaact gcatttgcaa cagtcaattc tggatgcaag tttattcgta gggataaaaa
    24901 agattagtgt tggcttatga tgcaagctct tttttatttt aggaattaga gagggatttg
    24961 tccttctatg attttttgct ataataggga agatgtttat tttatgaaag aatagttgag
    25021 ggaatttaaa tgaataaaaa attgatgcaa ggaacatttt ggctaacctt tgctaatctt
    25081 ctatgtaaag ttttaggggt agtctatttg attccctggc taagtatgat ggggaacaat
    25141 caagatggta tgcttgctac gacattgtat aatgttggct atcttcctta tggattgttc
    25201 ctgatgcttg gtacagtcgg atttccaaat gcgattgcaa aaaaagtagc tgtagccaca
    25261 aaagaaggag ataacgctgc ttgtcggctg atttttagaa gtacgatcaa tatcatgttt
    25321 gtcatcggta tcgcctctgc tgcactgatg tatttatttg ctcctttact agccggaatc
    25381 agtccgattg ctaacgttaa taatgggata ctagctatca gaagtttatg cccatctttg
    25441 attgctatcc caattcttag tgcaatgaga ggatatttcc aaggaaaaaa tgatctgcgg
    25501 ccttatggaa cttctttgat tatcgaacag gctgtgcgag tgatcgttat cttggctggt
    25561 acttattact tgcgcgtatt gactgacgga accatattag aagctgtatt gatcagtaca
    25621 gtcgcttctt tcttcggtgg gttagctgcg attatccata tgtatatcgt cggacgcaga
    25681 aaagacttct tcgaacttaa agattttttg atttccagcc gttatttcga aagagaaaac
    25741 cgatctgcct ctgtctctat cattcgggaa acactaccgt ttatttttgt cggctcagtc
    25801 attactatcg ttcaaatgat cgaccaagta acgatgaaac cactgcttca ctttttccgt
    25861 ccagagatcg cagatcagca actggaattc ttattcagcc gtgcttctgt caatccaaat
    25921 aaattgacat tgatcctgat ctccatggta ggaacagtag ctataagcag cttgccaata
    25981 ttaagcacga tgaagaaaaa agatcggata caaatcgaaa aaacagtagg agatagtttt
    26041 tcgattgcgt tattgattct tcttccttca ttgactggaa tgtctttatt agctggacca
    26101 ctatacacat tgttttttgg ctatgatcct gaaagtgtcg gatatttcca aatggctttg
    26161 ctagcttctt tgtttttctc tttattcacg atcctatcta cgatgcttca atctttgaat
    26221 caccatcgtt ttgcgatccg attaactgta gaagcaatta tcctgaaagt catcttccaa
    26281 gtaatcggat taggattagt tggcggatac gggatgagct tatcaaatgg cttagcattc
    26341 ggtattgtct ttgttcgcgg ctatcacttt atgtgcaaag aataccgaat agcaccttta
    26401 gcaaagatca gcaatttttt cttaaaaact ttccgctcca ccctgattat gcttgcagta
    26461 tgttttgccg ttttcttttt gctgaatcag cgattgtcaa tggaatcaaa aagctatgca
    26521 atgatttatt gtgtggctgt aggaagtatt ggcggcttag tatttttatt ttctcaattc
    26581 ggaaaaaatg ggtttcgaat gttgaaaaat gttaaacatc atagcagaag caaagaatag
    26641 gttaactaat cgttttttgc tgtcacttga aaaacggcaa ctcgtatgat tacgcttgtc
    26701 gtttttctat tttccaattt tagtttcata tctaatctta cttatgatat agttataagg
    26761 tctggaaaaa ttctttgaaa cgaggaaatt aaacacatga aacttgcagt agttacagat
    26821 agttcggcat atctgccgga acgtatcaag aaccatcccg acttattcgt tattccaatc
    26881 cctttgatta tggatgggaa aatttataac gaaggaatcg atatcgaagc ggatgaatat
    26941 tatggacttt taaaaaacag taaagatttt cctactactt cacaacctgt cgtaggggaa
    27001 gttttgggtt tatatgagga attgaaagag aaagggtatg acacgatttt aagtatccat
    27061 ctttcttcag ggatttctgg ctttgtcaac acattatttg cgatgaaaga tgacatcagc
    27121 ggagtaacgg tcattcctta tgactctaag atcactagta tgccgatggg acacatggta
    27181 gaagctgcct tagacttaaa tgatgaaggt cagtcccttg atgaaatact tgcacatatc
    27241 gatcgaatcc gagataatac ctatgcttat ctgattgtag atgatttgaa caatttagta
    27301 cgaggaggac gtttgacaaa tggtgcagcg ctgattggca gcttattaaa aatcaaaccg
    27361 atcctgacat tttctgaagg gaaaattgtt ttattcgaaa aaatccgttc aagtaaaaaa
    27421 gcatttgctc gagctgaaga aattattggt agaagaaata acgaaataca accgccagtc
    27481 aaactttacg tgatacatgc aaataattta gaagtagcga ttgcggagaa agagaaattg
    27541 cagaaaaaat atccaaatgc agaaatcgaa attggtcatt tcggaccagt cattggtaca
    27601 catttaggtg aaaaagccat cggcattgcc atttcagcgc aataattcct tttattcaaa
    27661 aaacatacca aaaata
//
LOCUS       DAHUVV010000028        27245 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.028, whole genome shotgun sequence.
ACCESSION   DAHUVV010000028 DAHUVV010000000
VERSION     DAHUVV010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.028"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(9..1577)
                     /locus_tag="NK767_001210"
     CDS             complement(9..1577)
                     /locus_tag="NK767_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCH6694217.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1581..1991)
                     /locus_tag="NK767_001211"
     CDS             complement(1581..1991)
                     /locus_tag="NK767_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCH6694218.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(1988..2911)
                     /locus_tag="NK767_001212"
     CDS             complement(1988..2911)
                     /locus_tag="NK767_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HCH6694219.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2908..3786)
                     /locus_tag="NK767_001213"
     CDS             complement(2908..3786)
                     /locus_tag="NK767_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCH6694220.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3773..5110)
                     /locus_tag="NK767_001214"
     CDS             complement(3773..5110)
                     /locus_tag="NK767_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCH6694221.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5113..6216)
                     /locus_tag="NK767_001215"
     CDS             complement(5113..6216)
                     /locus_tag="NK767_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCH6694222.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6290..7798)
                     /locus_tag="NK767_001216"
     CDS             complement(6290..7798)
                     /locus_tag="NK767_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCH6694223.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7764..8456)
                     /locus_tag="NK767_001217"
     CDS             complement(7764..8456)
                     /locus_tag="NK767_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCH6694224.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8478..9452)
                     /locus_tag="NK767_001218"
     CDS             complement(8478..9452)
                     /locus_tag="NK767_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6694225.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9752..10624
                     /locus_tag="NK767_001219"
     CDS             9752..10624
                     /locus_tag="NK767_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6694226.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11082..13280)
                     /locus_tag="NK767_001220"
     CDS             complement(11082..13280)
                     /locus_tag="NK767_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6694227.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13512..14504
                     /locus_tag="NK767_001221"
     CDS             13512..14504
                     /locus_tag="NK767_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6694228.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14564..15985
                     /locus_tag="NK767_001222"
     CDS             14564..15985
                     /locus_tag="NK767_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCH6694229.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            15978..17477
                     /locus_tag="NK767_001223"
     CDS             15978..17477
                     /locus_tag="NK767_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCH6694230.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17516..18910
                     /gene="uxaC"
                     /locus_tag="NK767_001224"
     CDS             17516..18910
                     /gene="uxaC"
                     /locus_tag="NK767_001224"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCH6694231.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19069..19752
                     /locus_tag="NK767_001225"
     CDS             19069..19752
                     /locus_tag="NK767_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HCH6694232.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19754..20590
                     /locus_tag="NK767_001226"
     CDS             19754..20590
                     /locus_tag="NK767_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCH6694233.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20601..22355
                     /locus_tag="NK767_001227"
     CDS             20601..22355
                     /locus_tag="NK767_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6694234.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22631..22972)
                     /locus_tag="NK767_001228"
     CDS             complement(22631..22972)
                     /locus_tag="NK767_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCH6694235.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23040..25211)
                     /locus_tag="NK767_001229"
     CDS             complement(23040..25211)
                     /locus_tag="NK767_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCH6694236.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25380..26279
                     /locus_tag="NK767_001230"
     CDS             25380..26279
                     /locus_tag="NK767_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCH6694237.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(26350..27150)
                     /locus_tag="NK767_001231"
     CDS             complement(26350..27150)
                     /locus_tag="NK767_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCH6694238.1"
                     /translation="MTIKKKLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGID
                     KTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNS
                     CIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAA
                     GPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLL
                     QKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
     gene            complement(27147..>27245)
                     /locus_tag="NK767_001232"
     CDS             complement(27147..>27245)
                     /locus_tag="NK767_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCH6694239.1"
                     /translation="KRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         8087 a         5621 c         4894 g         8643 t
ORIGIN      
        1 gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc agcataactt
       61 cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt aaaccctcct
      121 gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt tgatacgtct
      181 ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt cttcttttct
      241 gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc tatcatacgt
      301 aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt ggattcattt
      361 aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc aaatgctgtc
      421 tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc gaaggtagat
      481 tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt atcacaccaa
      541 tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc ttcaaatgta
      601 tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat cattatttct
      661 tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt ccaatacact
      721 tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct ggtacaaaga
      781 tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc tgtacattgc
      841 aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc aagctcgtaa
      901 ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc tgtaccgcct
      961 tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc ctccgttttc
     1021 gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt cttctctgca
     1081 tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg ataatcccat
     1141 agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga aggatcaaag
     1201 ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga tgcagtgatc
     1261 aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt gtaaacaaga
     1321 taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt tgtaggattt
     1381 ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt gactaaattt
     1441 ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat aatgtaatac
     1501 cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat aggatttttc
     1561 actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata taaactgaat
     1621 gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt gccaatcaac
     1681 aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt tcctttagca
     1741 gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac ggcaaacaga
     1801 ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga atgatctaca
     1861 gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa agtaactgct
     1921 aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa tagttcccat
     1981 tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt cttttgatcg
     2041 atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt ttcttgtcat
     2101 cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct tcaaataaga
     2161 catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca ggttctgcag
     2221 tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta gatgctgctg
     2281 tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga aaaacggcat
     2341 ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca taggtctgac
     2401 gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct ttttgcaaat
     2461 caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag ttttttaaag
     2521 atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct cctggacctg
     2581 ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag aaaaaagtag
     2641 cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca aaacgattac
     2701 cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg ctttctatat
     2761 attcatcata tcttccttct tttactatca agacctttgg atggctatcc ggcaggcctg
     2821 ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct atagcattct
     2881 ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt gtttccaaat
     2941 atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag aaaagttttc
     3001 tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca gtacgccttc
     3061 gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg gagcgatttt
     3121 ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt aataaaaccc
     3181 atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg ccgctagatc
     3241 aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata agcctcccat
     3301 agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct ccaaataatc
     3361 gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg ttaataaaaa
     3421 ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc aagcatcaat
     3481 cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag aagctaagta
     3541 aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt gagaatgcag
     3601 gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg ctggtactgt
     3661 ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt gtttttctcg
     3721 gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa aatcattcaa
     3781 ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat tgatttgatt
     3841 ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt ccctcgtgat
     3901 tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa aatccttgtc
     3961 tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca tcttgggcca
     4021 tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct aatccataaa
     4081 taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg atccttctga
     4141 atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac ggttctttcc
     4201 ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca ttatccatga
     4261 ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta gatttcaatc
     4321 gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta cgaatactac
     4381 cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg caatttgtga
     4441 tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc ttgattgcaa
     4501 tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg catgattctg
     4561 gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag ttatcaaacg
     4621 ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc aatttaatat
     4681 ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga ggataagcga
     4741 gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca tctaatgtac
     4801 cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa gcataaactt
     4861 ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga tcgtcagaga
     4921 atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa aataaagctc
     4981 ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt gctgcagcat
     5041 caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca aatgtcaaaa
     5101 tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg caccaacgcc
     5161 tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac catctcgctg
     5221 atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc agccttcttt
     5281 tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt gtggaagcca
     5341 ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa caaacatact
     5401 actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta cttgatacca
     5461 tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt ctagaatttc
     5521 tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg ctgcagccat
     5581 caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt ttggatcagc
     5641 ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt ttggatcgaa
     5701 tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt tttcttcact
     5761 aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat ccaaccaaat
     5821 ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg gatggtttgc
     5881 caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg tttctttgta
     5941 caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt caacgctgta
     6001 gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta tgaaatcaaa
     6061 ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta cgacgccata
     6121 gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg ttctttccat
     6181 gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt taaaaattaa
     6241 aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat tactgtgccg
     6301 ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt gctttccagt
     6361 cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat tgcaccgtag
     6421 catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt gaatccaatg
     6481 gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga ttagagaatt
     6541 ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga gagcttgata
     6601 acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa ccatcatcat
     6661 tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca tcttcttcta
     6721 aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt acttgagatt
     6781 tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga taaccgccgc
     6841 cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga ataccttgac
     6901 ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt cttccttgtg
     6961 caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc aaatagccat
     7021 tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta tcaaattctg
     7081 gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa taagaagtga
     7141 aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa ccaactgtgt
     7201 cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact tcataaagct
     7261 catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt cgaatgacta
     7321 agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg tcgatactcg
     7381 cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt aaatattctg
     7441 atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt ttcaacatcg
     7501 caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt gttaatcttt
     7561 cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt ttttcttcga
     7621 tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt aaccaagtga
     7681 ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg ccgcatgcgg
     7741 caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct tttttcataa
     7801 attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc tgtatatgag
     7861 ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc ggaaaagaag
     7921 agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat cagataaatc
     7981 cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc gaataaaaat
     8041 gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat tgggaaaata
     8101 tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct aagataccaa
     8161 ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata tatccagctc
     8221 atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt cgtcaaagga
     8281 aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata cgatgcagga
     8341 ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa attcaaataa
     8401 accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg cagcatatct
     8461 ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc aatcttatta
     8521 gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc tgtactgaaa
     8581 cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat ttctgcaacg
     8641 cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc taatgtctga
     8701 ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa tgtaacatgc
     8761 catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa ctgtgtatcg
     8821 actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt ccagatcact
     8881 tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat tttttcaaat
     8941 cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa tacataagta
     9001 atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt ttgaatcccg
     9061 tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat tggcatcgga
     9121 aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa tgtaaagaat
     9181 gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact tcccattatt
     9241 cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg aagataccta
     9301 aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt attcgttttg
     9361 attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga tagcgtcagt
     9421 tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt gtgaatgttt
     9481 gctgcttatg acttcatgat agataactat ctgaattacg tatataatca tttttaccgt
     9541 aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa agtgtatttt
     9601 ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca cttagcatag
     9661 gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga aacagaaaca
     9721 tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta taggagaaag
     9781 aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct gtatcgttcc
     9841 ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg cgaaaaagtt
     9901 tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt ctacgccgac
     9961 catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga tcgtcagtat
    10021 gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg gtcgaggttt
    10081 cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc caacgttctt
    10141 agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc ctgtggctgt
    10201 taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg atagtttgga
    10261 agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa ttatgctgcc
    10321 attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt actggaacac
    10381 ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac agattctttt
    10441 acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg ttgatgtgat
    10501 tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc ctatgctgat
    10561 tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt ctgtcaaagg
    10621 gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct tcaagaaata
    10681 agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt atttttcgaa
    10741 gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca gccttgagcg
    10801 atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata agaagcgatt
    10861 ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag cctgtctctt
    10921 gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt tctttttgcg
    10981 tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt ctaacttctt
    11041 ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata tttttttcga
    11101 tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt ttgcggattt
    11161 cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt caaccatttt
    11221 tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc tgtttccttt
    11281 ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat cacatctaaa
    11341 gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga caaattttta
    11401 aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa ttgttctaaa
    11461 acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata aatttgtttc
    11521 atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac gagttcattg
    11581 atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc aaacaatgca
    11641 tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat ggcttcaaac
    11701 aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc agcaggaacg
    11761 atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata cagggcttct
    11821 tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt actgatgcct
    11881 atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg gcaatactcc
    11941 atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt ggcttgcatt
    12001 tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc cgtgatcatg
    12061 ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc taacagatcg
    12121 atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa ttgctgcaac
    12181 cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac aaatagcgtt
    12241 tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc tcctatgatc
    12301 cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt tgatttaaga
    12361 ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa taagtaggaa
    12421 agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat tctcagattg
    12481 cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca attattataa
    12541 tctgatttca gtactatata cgttttatct ttttctctta tcactttcag ctgattgtaa
    12601 tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt ctgagtagta
    12661 aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc accaacaact
    12721 tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc tttttcaaac
    12781 attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa taaattattt
    12841 ttgttattta tgtacttctg ttgatacttt tcaatttcct cttcggtcag ttgactcaac
    12901 gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt attatcatcc
    12961 ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt ggagtaatct
    13021 gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc atacgtagaa
    13081 aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg cctgagcaaa
    13141 ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg gtaaatgaat
    13201 aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa tattttataa
    13261 aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag gctttcattt
    13321 ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa gaatagcata
    13381 attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat caaagatcta
    13441 ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga aaaagtaagg
    13501 aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc agctggcgtt tctcatacga
    13561 cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa caaaaaatac
    13621 gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt ttagtgaatc
    13681 aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact tcatcaaatt
    13741 tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac agtttatctg
    13801 ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac gatggactga
    13861 tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa gaaaaagaat
    13921 ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac gtcactgccg
    13981 atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga catacgaaga
    14041 tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa aaaggggtat
    14101 tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta acaggagatt
    14161 acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct gacctgccta
    14221 ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct gtacatttag
    14281 ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct ccagtcgtct
    14341 cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg tgtcaagaag
    14401 gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct aaaattgagc
    14461 tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca agtaatttta
    14521 tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa aactgacaaa
    14581 agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg aaggcaattt
    14641 tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat tgtttaaagg
    14701 taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga accagcaaga
    14761 taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg atacttccga
    14821 gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg cttatctatc
    14881 tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg caggaatcca
    14941 attcgtggag actgataatt taacagatac tcccccacag agcctcccgg gcaaactgac
    15001 agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca ttccttgcga
    15061 actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg cagaaaaatg
    15121 ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct gttgtagttt
    15181 agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca atcaacggca
    15241 taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg tgatcgaagg
    15301 gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg tcatcatcac
    15361 agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac cgcatacagc
    15421 gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa tgaaagatcc
    15481 tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta tgctttcttt
    15541 acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct tgaatccttt
    15601 tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca gcacccgtct
    15661 gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt taatcactgt
    15721 ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac atgatgatga
    15781 aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta ttcctcgatt
    15841 attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg tgacagatac
    15901 agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat tacaagaaat
    15961 gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta aatcgacgag
    16021 acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg attgatcagc
    16081 aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg ggtgatttga
    16141 aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg acagacgtaa
    16201 ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt gaattggatt
    16261 atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga acatttcaag
    16321 gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta gtacctaccg
    16381 ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa aaacatccag
    16441 atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt tcgcagcttg
    16501 gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat cccaatgccg
    16561 gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa ttcaaaaaaa
    16621 ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat gtttacgatg
    16681 agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa aatgaccagc
    16741 gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt tctgatggtc
    16801 tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg attgctcaag
    16861 gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa gtattgatgg
    16921 cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat gacttcaaac
    16981 agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt aataaagcag
    17041 gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca ggaagctctc
    17101 ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt agtttattgc
    17161 aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat tgccagttgg
    17221 tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg gtcaaggtat
    17281 ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat gcaggcgtct
    17341 tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc atcgctgtag
    17401 ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc atttttaaaa
    17461 acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa gatttatgtt
    17521 tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc attcctacgc
    17581 aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa tttacgaaaa
    17641 caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca aatggcgctt
    17701 gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg attttgaaaa
    17761 atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt acagttggtc
    17821 acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag aaaatgcaga
    17881 gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc gtcgggaaat
    17941 cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag acgacttgca
    18001 ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt cattccgtcc
    18061 agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac tactggaaga
    18121 agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag ggcagcggat
    18181 agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca tcttacgcta
    18241 taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag ctaacaaact
    18301 attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt tgacccactt
    18361 ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta actgtaatac
    18421 acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg atctttcttt
    18481 gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt tgccaaagac
    18541 gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga tgggctgttt
    18601 ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca acgatactcg
    18661 agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg ggaattttgt
    18721 cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat atttccgtcg
    18781 tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg aagatgaaca
    18841 acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt actttggctt
    18901 ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt gaaataacgg
    18961 aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt cttcttctct
    19021 tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat gcctactatc
    19081 catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc cgagacaggt
    19141 tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa ccaagcaaag
    19201 aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct agcttcacag
    19261 tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa aatccaagaa
    19321 gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt ttttgaatgc
    19381 gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg agattactta
    19441 gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat gcgtgcattt
    19501 gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga attgttccag
    19561 accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga tacttgtaaa
    19621 aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc tgataaagtt
    19681 gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa ttatttacaa
    19741 aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt tagatgttcg
    19801 aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata tccaatttgc
    19861 accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg ggagtgctgc
    19921 ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag gatgctatat
    19981 caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt tcaaacagca
    20041 aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg gtagtgtctc
    20101 tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca gaaattctat
    20161 cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg aagcagggat
    20221 caatcatcca atctataact atcagttagc taaacgttta atcgatgaag tagcttcacc
    20281 aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc atgctgatca
    20341 ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag cgcttcatct
    20401 caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca agggctgttt
    20461 agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt acgccactct
    20521 tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt ttatttacca
    20581 agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa aaatatcatg
    20641 caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt tttgccatgt
    20701 caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc gctattgcca
    20761 atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt tatttcaacg
    20821 cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt catgtaaaat
    20881 accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt atcaagaaaa
    20941 tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc cagtcaaaga
    21001 tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc agcggattaa
    21061 atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt cctgttgttt
    21121 tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt ttccgtaaaa
    21181 ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct gcaaaagtga
    21241 tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa cacgaaacaa
    21301 ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta gatatcctgc
    21361 aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg atttgtttga
    21421 ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc gtgatgtatc
    21481 agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg attcctacga
    21541 tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct ggcgatgtag
    21601 aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt tttgaggatg
    21661 tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct ctagaggtga
    21721 aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct acactcctta
    21781 acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat gatcagcctt
    21841 tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct caaaccactt
    21901 tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac gtatcaaaag
    21961 aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac cagcttcctg
    22021 atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc caaaagcaac
    22081 gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta gatgaagcaa
    22141 cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat tacctgacag
    22201 aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag gcagataaaa
    22261 tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa ctgttagctc
    22321 aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct atgacaagac
    22381 ttttgtcgta gctcctattt ctgaataaac ggtgttctcc tgccaactcc tcggtcacaa
    22441 gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa ttgttccttg taactcagag
    22501 ttaaacggta ggctcacgac ctcttggtaa atggtgttca agagacagcc tatcggcaat
    22561 aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt tcacaccctc tgttgtttca
    22621 taaaatagtt ttattcgctg taaaggtcgc gaactggtgt catgatgata cggttcaagt
    22681 cattgatgat tgtactgaag ccttgctctt tttgcatcaa gtcattgatt acttcttctg
    22741 attggatttt cacagccatt tcttgtgctt tcattgcatc ttcgtcgctg aactcttgtc
    22801 cactcatttg tttttcttgt aattcgattt gcatcgcttg gaagtctttg aacagtttgt
    22861 gtgcttcttc gtttgcttgt actttctcgt acgcttcttt caacgctttg aattctggta
    22921 attcacggat ctcacgttcg atttgattcg cgctatcgta gatgtttgac atgttatgcc
    22981 ctcttttctt ttagtagtta ctctctattg taacatgggg ttattcgttt tcaaaggggc
    23041 tactctcctt gatatttccg ccagaggtct ttagccgctt caccaattcc ctgaacgcct
    23101 tctttcacca tatcgccgaa gtttttcagc ccttcttttg cttgttcacc atattgattc
    23161 aagttatctt gccaatttcc agactctcca ttagaatttc ctgtgtttcc aacttgatct
    23221 gcagctacca cttgtcctcc tgtggcatag gcatctgcta cactaaatcc tgtttttgaa
    23281 gtatacggaa ggatgccttc tgccgctgat ttgaagactt tcccgacaac atttccactt
    23341 gtcccttcca aatagtgcag cttgctggat tcttcgtaac ccatccatgt cgaaatcact
    23401 acgtctggcg tgtaaccgat catccattgg tcatttgctt ttgtggcatc gaaattagtt
    23461 tctgtggttc ctgttttccc tgctatcgta taacctgcag gattcgcagc agccgctgtt
    23521 ccatttgaaa atgtaccaag aagcatgctg gtcatttcat cggctgtttc ctttgatatg
    23581 acctgttttg gttttttgtt tgtgttatcg acgatcaccg ctccagtcga atcaacgatt
    23641 ttcgtgataa gatgcggcgt atacatctga ccatcattgg caaatgcact gtaagcaccc
    23701 gccatgatca atggagatac tcctttttcc agtccaccaa gggctaagcc ataatactta
    23761 tcagattttg ctaaaggcag gccgaattct tctgctttat catatccttt ttgtaaaccg
    23821 atctcatgca aaagccatac agtaggcaaa ttcaaacttt gtgcaacagc ttgatacatc
    23881 ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat cgtaataact ttgcggttcg
    23941 tctttcaaga tactggaagg cttgtatcca gcttccagtg ctggtgtata tacgcttaat
    24001 ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag caaagctgaa tcctctaaag
    24061 acatgatctc ctcttcgtcc tactagtgct tgtacaccac ctgtattcgg atctaaagct
    24121 actgaagcag attggaccat ggcaccatct gaagcatttg ctgggaataa tgcactgttt
    24181 tgataagtag cttctaattg cttttgataa tcctgatcta aagctgtata gattttatag
    24241 cctttattca tgacatcttc ttcatcaaga ccatactcgt taactgcttc atcgatcact
    24301 gcatcaaaat aataaggata acgataacca gaatctgaat tatcgtatgt gtcattaaga
    24361 tacgcgctga tatctgtctg gctttctgct tgttcttgtt cttttgtgat ttttccgttt
    24421 tcttccatta cacttagtac tgtgtttttc cgatcattcg cattttttgg gtgatcgatc
    24481 ggattataaa taccaggacc tttcagcatt cctgccaaag tagcagcctc agacagcgta
    24541 acattcgttg catctactcc aaaatacttt ctggaagcgt cttggacacc ccataccccg
    24601 ttgccaaaat aggcattgtt aagatacatg gtcaagattt cttctttgct gtacttcttt
    24661 tcgatctcga ttgccaaaaa taattccttc gcttttcgat caaacgtttg gtctaatgtc
    24721 agatacgcat ttttggcaag ctgctgagtg atcgtacttc ctccgccccc tgatgttccg
    24781 ccagacaaga ccattcggac tgctgcacgg gcaatccctt tgatatcaaa cccgtgatgc
    24841 tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt ggatataggg agaaatcgcg
    24901 tttaattcta catatgttcc tttttgggcg tacagtgtgc cagcttcttc gtttttctta
    24961 tcataaatga ctgtcgattg gcttaatcct gattttaacg tttcgacatt tgcttgtttt
    25021 gctaaaataa agaggtagat acttgtcact aagacacaga tcaaaccaat cagcaaaatc
    25081 aatttgttca catgatattt tttccagatg cgctttcgcc atcgatggaa ttgtccgaga
    25141 tatggtctga tccaacgcca aaaactgatc aaagcagctt ttatcttttc taaaatgctt
    25201 tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa tcgtcttatt ttaccatagt
    25261 gaaagagtgt tttcttcctg cttaagttgt agttcaatat gtttttttac gttagtgcaa
    25321 gtagagtaac ataagaatga acagaaaaat gtaagttttt tcttaaatat gttttaaaaa
    25381 tggtacaatt agtctgcgat tgcactgaaa ggagtacact tgtgaaaatc acgatcactt
    25441 tacctatgaa ccatcctgaa acaacaattc gcgacttgct tgaaaaggaa tggttagttc
    25501 ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt ttggatcaat caacaacctg
    25561 ctttgttcca tcatgaagta aagggcaatg accagatcac attgttattc gaagaaacag
    25621 attataatta tcaagaagtt caattaggag atgcgaagca catccgtgtt ttatatgaag
    25681 atgagcattt gattatagtg gataagcctg caggaatgaa gactcatcca aatgaaccga
    25741 cagaaaacag taccttactc aatcatttag ctgcttatct cgacactaaa cagcaacgac
    25801 cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc cgttttattt gcaaaaaatc
    25861 catttgtctt gccgatactt ggaagaatgt tggaacaaaa aatgatctat cggagatatc
    25921 aagccatcgt atggggagaa attgccaaag atatgaccat caccaataaa attggacgag
    25981 atcgccatga tcggagaaaa agggtcgtag atgataaaaa tggtcagagc gccttgacac
    26041 atgtcactgt caacaaagta ctttctgaac aaacacaagt ctattgtgtt ttggaaaccg
    26101 gacgtacaca ccagatcaga gttcatttag ctcataaggg acatccgatc gttggagacc
    26161 ctctctacca aaatagaaca gctgaacgac tgatgctcca tgcattagaa ctgcacatga
    26221 tccatccatt tactcaagaa aaaatagtcg gtaaagcttt acctggatta tgggaataga
    26281 aataaaaaag aacagagtct tatcagcaga taatgataag actctgttct ttttgtgtaa
    26341 tttgtctgct tacttttgat gttcgtgttc tgctaacagc aaactaccga taatcccact
    26401 ttcattcggc agtccccaac gaacgatata ttcacttact tgaggtgttt ccatataacc
    26461 acccatcaat tcaacaaatt gctgacggat tttttgaagg agatgatcct gattcattac
    26521 cccaccgcct aaaataatct tttcaggtgc taacgtcaac gtatagttga tcagtgcttg
    26581 agcaatataa taagcctgaa tagcccaaac aggatgatct tgcggcagat cttgcccttt
    26641 gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat ccttctaaac aatctttatg
    26701 ataaggacaa gtaccttcat aatggtcatc tggatggcgt ttcacccaaa tatgtcccat
    26761 ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc acgacacctg caccgattcc
    26821 tgttcccacg gtaaggtaaa tacaactatt ttttccttgt gctgctcctt gcttcaattc
    26881 cccatatgca gcagcattga catccgttgt ccaagcagca ggaatattgt agcgttcctt
    26941 gattgtgcca agaaaatcga attgtgccca cccttttttg ggagttgcca aaacgtatcc
    27001 gtaattatct tttgttttgt cgataccaat cgggccgaat gagccaatcc cgattccctt
    27061 caattcataa cgatcaaaaa attcaaatac ctgatccaat gtttcttctg gtgttgtggt
    27121 agggatactt aattttttct taattgtcaa attaagttag acagatcatc aatacttacg
    27181 taactcaaaa atacttccaa aggtgttcga taatccagtg attttctagg aatattattt
    27241 cgttt
//
LOCUS       DAHUVV010000029        25536 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.029, whole genome shotgun sequence.
ACCESSION   DAHUVV010000029 DAHUVV010000000
VERSION     DAHUVV010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.029"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            128..2188
                     /locus_tag="NK767_001233"
     CDS             128..2188
                     /locus_tag="NK767_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HCH6694240.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2271..2840
                     /locus_tag="NK767_001234"
     CDS             2271..2840
                     /locus_tag="NK767_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6694241.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3015..3821)
                     /locus_tag="NK767_001235"
     CDS             complement(3015..3821)
                     /locus_tag="NK767_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694242.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3959..5473
                     /locus_tag="NK767_001236"
     CDS             3959..5473
                     /locus_tag="NK767_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HCH6694243.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5876..6859)
                     /locus_tag="NK767_001237"
     CDS             complement(5876..6859)
                     /locus_tag="NK767_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HCH6694244.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6864..7511)
                     /locus_tag="NK767_001238"
     CDS             complement(6864..7511)
                     /locus_tag="NK767_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCH6694245.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7596..8228)
                     /locus_tag="NK767_001239"
     CDS             complement(7596..8228)
                     /locus_tag="NK767_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCH6694246.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8244..8795)
                     /gene="coaC"
                     /locus_tag="NK767_001240"
     CDS             complement(8244..8795)
                     /gene="coaC"
                     /locus_tag="NK767_001240"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HCH6694247.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8792..9529)
                     /locus_tag="NK767_001241"
     CDS             complement(8792..9529)
                     /locus_tag="NK767_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HCH6694248.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9526..10515)
                     /locus_tag="NK767_001242"
     CDS             complement(9526..10515)
                     /locus_tag="NK767_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCH6694249.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10773..11315
                     /locus_tag="NK767_001243"
     CDS             10773..11315
                     /locus_tag="NK767_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6694250.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11332..12417
                     /locus_tag="NK767_001244"
     CDS             11332..12417
                     /locus_tag="NK767_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6694251.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12429..13235
                     /locus_tag="NK767_001245"
     CDS             12429..13235
                     /locus_tag="NK767_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6694252.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13236..14063
                     /locus_tag="NK767_001246"
     CDS             13236..14063
                     /locus_tag="NK767_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6694253.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14060..15133
                     /locus_tag="NK767_001247"
     CDS             14060..15133
                     /locus_tag="NK767_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCH6694254.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15561..18257
                     /locus_tag="NK767_001248"
     CDS             15561..18257
                     /locus_tag="NK767_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCH6694255.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18334..18822
                     /locus_tag="NK767_001249"
     CDS             18334..18822
                     /locus_tag="NK767_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCH6694256.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18834..19328
                     /locus_tag="NK767_001250"
     CDS             18834..19328
                     /locus_tag="NK767_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6694257.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19349..20158
                     /locus_tag="NK767_001251"
     CDS             19349..20158
                     /locus_tag="NK767_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6694258.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20155..20976
                     /locus_tag="NK767_001252"
     CDS             20155..20976
                     /locus_tag="NK767_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCH6694259.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21027..23126
                     /locus_tag="NK767_001253"
     CDS             21027..23126
                     /locus_tag="NK767_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HCH6694260.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23123..24241
                     /locus_tag="NK767_001254"
     CDS             23123..24241
                     /locus_tag="NK767_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCH6694261.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24280..25158
                     /locus_tag="NK767_001255"
     CDS             24280..25158
                     /locus_tag="NK767_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCH6694262.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25349..25501
                     /locus_tag="NK767_001256"
     CDS             25349..25501
                     /locus_tag="NK767_001256"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCH6694263.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8067 a         4449 c         5407 g         7613 t
ORIGIN      
        1 acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga ttttatttat
       61 ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta gggaggacta
      121 agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt gtcgtttaga
      181 taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca cgatgctgat
      241 cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac aaaccatcaa
      301 ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta ctcatacttt
      361 cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata aagtcagtta
      421 tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga catcggaaac
      481 cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa aaggtgatca
      541 gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg acggtggaag
      601 cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa agaaaatcga
      661 tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga agaacgcaga
      721 tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg tggatacgac
      781 taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt taaaaacaat
      841 gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct
      901 tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg taacaaaaag
      961 cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag atactacaac
     1021 tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa atgtggcaga
     1081 aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca tcactcattt
     1141 tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta gtgtaaatcg
     1201 tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata tagatttatc
     1261 gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag caaacggatt
     1321 agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac tgatgaataa
     1381 taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag acaatcgttc
     1441 attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat
     1501 ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat cggttacaat
     1561 caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt ttacgattat
     1621 gtatagttat catgacccgt attcactcca agaactccat gctcagtcac tattacatgt
     1681 tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc
     1741 agaaaaaaaa caacaaatgg cccatgcagt ttctccaaca gggaaagtaa aacaaacaag
     1801 aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa cgataaggca
     1861 agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag agcctaaaat
     1921 aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga aggaatcaga
     1981 tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg atgagcatct
     2041 gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag gtattccttc
     2101 ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa
     2161 tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag tcaaataagt
     2221 agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa atgttagata
     2281 aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat gttccaatca
     2341 cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa tctgcttggg
     2401 cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt gagtattttc
     2461 aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt gtagagacaa
     2521 aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt
     2581 atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag
     2641 cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga ccaatctatc
     2701 ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg ggttttactt
     2761 ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt atgcaggttt
     2821 taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg gataaaaaac
     2881 gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata actaatggca
     2941 tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc ccttctcttg
     3001 ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat gaacgtaaat
     3061 ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac tgctccaata
     3121 aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta accattttgg
     3181 atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt ccgagtgata
     3241 tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt gatttgctgg
     3301 atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa ttccacaaac
     3361 tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag taataaagga
     3421 gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa gccgacaccc
     3481 ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa atagccataa
     3541 aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa aatgatttct
     3601 agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat ttcaaataaa
     3661 attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac ctgatccact
     3721 agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat tctacctgca
     3781 gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact ccttcatgta
     3841 tatccttaat tatactaaga agcgtgtgtt taccaaagct atatgactta aagaaaaaca
     3901 gagttgatat tgtgaaaaaa atcacttaat atagtataag gagaaaaggg ggatacaaat
     3961 gaaaaaatta gttgctggtt tattaacagc cgttgcactt cttgctatga gcgcatgcgg
     4021 cgcagatcag aaaaatacag cgcagacaag ttctagtacg aaggaatctg ccgaagtatc
     4081 atcaggcgct tctgaacaag tatatacaga tccaaaagaa atgaaagaat cttatgatgt
     4141 cgtgattatt ggagctggtg gtgcagggat ggcagcagct atcgaagcaa aagatgctgg
     4201 gttgaatcca gtaattttgg aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc
     4261 aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa
     4321 tgatgccttc tatgaagaaa cgctaaaagg tggtggagga acgaatgata aagaaatgct
     4381 tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa
     4441 actggacaac ttaacgatca ctggagggat gagtgaaaag aggacacatc gtccgtctga
     4501 tggctcggct attggtggat acttagtgga ggggttattg aataacgtcc atgagcggga
     4561 aatccctatt tttgtcaacg cagatgtgac agacattaag aaaaatgggg aaacggtcaa
     4621 tgaagtgact gtacatgttc aaggagaaga accaaaagag gtcaccggaa aagcaatcgt
     4681 agttaccaca ggcggttttg gtgccagcaa agaatttatt gaaaaatatc gtcccgattt
     4741 agctgattat attacgacaa atcaagaagg atcaactgga gatggtatca ccatgattga
     4801 aaaattaggt ggacaaaccg ttgatatgga taaaatacaa attcatccaa ctgtgcagca
     4861 ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca
     4921 agaggggaaa agatttgtca atgaaatgga tacaagggat aaagtatctg cagcaattac
     4981 agcattgcct gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac
     5041 agctattgaa ttttatgacc aaaaaggata tgtcacaact ggaaagacaa tggaagaatt
     5101 agcagaaaaa atcgatttac caaaagaaac actagcaaaa acagttgatg gatggaatac
     5161 ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc acagccatgg aacatgactt
     5221 atcgtctccg aattattatg cgatcaaaat cgcaccagga atccattaca cgatgggcgg
     5281 tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa aaccagccaa ttagggggtt
     5341 atatgctgct ggtgaagtca caggtggctt acatggaaac aaccggatcg gtggaaattc
     5401 agttgcggag atcattattt ttgggcgaca agcgggcata caagcagctc gatttgcttc
     5461 ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta
     5521 ataccgaggg tcagctgatt gaacactgtt tagtgaagag gttgtgaaaa agctgccctg
     5581 catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttaac ttggttggac
     5641 ttattaccgc agatgcaagc ttttgaacac cgtttattcg gaaaaagagc gtgggacaaa
     5701 agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag cagaagaaat
     5761 tccttcggaa ataagctgaa attcacaaaa atttgaaaag caattttcgt gaatttcttc
     5821 ttatttcttg gagttaaacg cttctgtccc agcctctttt ttgatcagca tgattttatt
     5881 cagttatctt caaaatgatt tttccgatgt ttttgtttgc ttccatatat tcatgcgctt
     5941 gtttgactgc gctgaattgg aatgtacggt caatgattgg ccgaatcttg ccattttcta
     6001 gaaaaggccc agctttttgc ataaaagttt gtgcgagctc tgctttatac tgatcacttc
     6061 taggtgtcaa taatgtacca agcagttgga cacgtttccc aatcaataca ccaaggtcta
     6121 tctcaggcac gatggtccca cctagcatac caatcaagac ccaacgtcca tcaacggcta
     6181 tagaagctaa attcttttcc cagtaagaag cgccgatgaa atccaaaata acatcgactc
     6241 cccgaccttc agtgattttt cgtacctctt cttgaaagtc ttgtttttta tagttgatca
     6301 attcatctgc accaagttct ttacaaaagg cgagtttttc ttctgatcct gcagtaacga
     6361 tgattttcgc gtgagaaagc tgtctagcta attggatcgc tgctgttccc acaccacttg
     6421 ctcctgcatg gatcaagaca gtttcgcctt cttgtaattt tccaagccag taaagtgttt
     6481 gataagcagt caaaaaaact tctgagattc cagcagcaga aacatagtct agcgtatcag
     6541 ggagaaggat tgcacgatcg gcaggcatca cagcatattc ggcataacca cctagattga
     6601 ctaatccgta tacttttgtc ccaggagtaa aagtggagat atcactttta ttctcaacta
     6661 cctctccagc tacttcgact cccaaaatag ggtagggagg tttcaaagag actgattctc
     6721 ttttcataat atctgttcga ttgacagctg ctgtatggac tttgatcagt aattcgcctt
     6781 gttttggttt aggaacaggc acttcaattt gttctaatac atctacagaa ccaggatgct
     6841 tgattgaaat ggcacgcatg gatctattcc tcctttgccc attcttggaa tgctgtattc
     6901 aactctttgc taccttcaga aagtactaga gtcgtacgag aaatatccgt ttcttccact
     6961 agagcggcaa gtacagcagc aactgtagag cgagtgatcg tttttgatgc cttctcatta
     7021 ggcttagtta attgaatgga agtaacttct tcatcgttag tcaatgtgac tggttgaaga
     7081 atgacaaaat ctaagttagt tcgtttcagc cattcatctg cataatgttt agtaatataa
     7141 taatctgtca tggactcagg ccattttgct ggttcatctg cataaacagc actgaccatc
     7201 agatagcgag agacatgcgc attttcagct gcgatcactg ttttgattgc gccatccaag
     7261 tctacttgca gaagattctt tccttgagag ccggcagcaa aaatcaatac atcgatgcct
     7321 ttgaatgctt cggtcatttt ttccacggac caagttaaat caaaagagac atacgaaaca
     7381 ttctcttttt ttactaatgt ttgggaagca acatctctca cccctgcaaa aatttcatgg
     7441 gaacttggag ctaaatcttt tatcaggtgt cggccaattt gtccgtttgc acctacaaca
     7501 aaaattttca tttggatcat cctttcgacg tcaaatcttt ccattcaata gtaacaccaa
     7561 aagagaaaaa aatcagagat cggctacgat gactatcagt tagtaagaga agggtgcttg
     7621 aagtatctta acaagacatt gcatactgct gcagtcgcaa gacctgcgac gattccttca
     7681 ggtataccac ttgtaaaaat aaccgctaga atagctgaat aaacagcatt taaatttgtt
     7741 ccgatgactt tggcataatc ttgctggaat aaaaagtaga tcatattcat cactaaaatc
     7801 gtattggtca atgaaccgga aagtccagct aagaataaag aaacggaatc tgctttttgt
     7861 ttcattagtt tgcgcagacc tttataaacg aaataaggaa ccaccccaat caagatgcga
     7921 ggaataaacg cgatcagcaa ggctttccag cttccatgtt ctgttccgat gactgggata
     7981 aacggagaaa agacaaaaga caaaggtgtt tggataactg tgctacggat catgctgatc
     8041 agtccaaatg taccgccaag aaaagcacct agtcttggtc ctaaaacgat cgaagcaatg
     8101 attacaggaa tatgcatggt tgttgcattg attggaccga tagggataaa acctaaaggc
     8161 gtaacagcta ataaaatcaa aatagctaaa aacatcgcag ttaatgtaaa gttttttgta
     8221 tttttcataa gtactccttt tttctaagca tttctttcat taagagtcag catgacctgt
     8281 tcgatgatcg tctgaatatc agctaaggca ccttttccaa aatctccaca agctaaaaga
     8341 gattctcttg gttcaatttc ctgatagccg acagatctca atgtttccag attacgctga
     8401 gtgatcgggt tttgatacat attcgtgttc atggcagggg caatcaattt tggtgttccc
     8461 actggaagcg ccattgcgac tgttgaaaga agatcgtctg caatgccgtt tgctagcttg
     8521 ccgataatgt ttgctgtagc aggcgcgatg agaaacaagt cagcttgttt tgctagttcg
     8581 atatgattga ttacctgcgg atttgattct tgcatgacgt ctgtatggac tgcgcgcttc
     8641 gacagagatt gcaaggttaa tggcgtgata aaatgtgtgc tgcttttaga cataatcaca
     8701 tctacctggg cacctaattt gacgaattga cttgtaagat cagcgctttt gtaagcagaa
     8761 atacttccag taacgcctag taagatcttt ttcattttga ttcctccagt ctaattttct
     8821 cagtaatcaa tgcagcaatc tcagatttac tttgagcttc ttccactgtt ccatctttag
     8881 ataagagata gccgtggtgt gtctcgccat ggatttccgt caaatcattt gcgaaaataa
     8941 aatcagtttt gttatttatc agactatttt ttgcgactgc cagaagagat tcctttggta
     9001 catctactaa tagtttgaaa ccaatgattt ttgcttttgg ctgccattca cgtagataag
     9061 aaatgatttt tggtgttttc ttcaaaacga gcacaagatg ctcggtgtta gaagagatct
     9121 ttgaaacagt ggtatctgtc tgttcatttt ccgcaaacca attgtccagt tcttcttgcg
     9181 ttgaagaagt gggtaggttt tttgccagct gttcttctga aaaactaaaa gcaggagtaa
     9241 aatcactgac tgccatactg tggataatcg catcgtagtg ttcttttttc gttagttctt
     9301 ctaattgtaa aaataagtcc agtgcagatt cgatcgtata aatcgttata ttggagtgtc
     9361 ttttcggttt cagtgcttgt ttcgtagtca cgtaatcgat tgtggtatcc ggattggcag
     9421 ctaaataatc agctaatgct tgccctaatc gtcctgtcga atgatttgta atagcacgta
     9481 cttgatcgat tttttcagtc gttcctccag ctgtgattag aatcttcatt tatggtcacc
     9541 tacatttact agtgttcttc cttggtgtgt tccagcaagt agtttagatg ctgtttcagg
     9601 caattgatcc agagtaatct cttggacgac cagttcatct gcaatctgca aatccgccat
     9661 ctgttgccat aaagaaagac gttgatctat tggtacattg acagaatcaa ttccaatcaa
     9721 ctggatgttt cgcagaataa aaggcaagac agtcgcgttt agtttgattc ccccagcatt
     9781 gccacataaa gctactgcac cgttataaga gatcaatgga agaagactgc ttagctgctc
     9841 gccacctact gtgtcgatga catagtcgat ttgctgtttt cctaaagctt ttgttgtctc
     9901 tggcagaaat tcatcaggtc gtacgacttg caaagcacct agacttttta gccatgtgac
     9961 agactcttct tttctggaaa aggcaatgat tgactggaat cctagtttat gtaacagggc
    10021 tatcgctgta ctgcctacac cgccagaagc accagtaacg acaacttttt tatcggaggt
    10081 cataccttga cggattaaag cgttcacaca caacatagca gtaaaccctg ctgtaccaag
    10141 gatcatcgct tgacgcagcg tcatattttt tggtagagga actaaccagt ctcctggtac
    10201 tttttgatat tggctataac cgcctggatg ggtgacgcca agcccaaaac cagtagcaag
    10261 tacaagatct tcttttgaaa aacgtggatt gttactttgg acgacagttc cggataaatc
    10321 aatccctgga gtcatcggat atgttcgtat cacctgacca gactcgctca ttgccaatgc
    10381 atctttataa ttgacatcag aataggcaat ttttaccaag acttcatttt cagccaactc
    10441 agtaaatggc agatcactga ttgctgtatg gaaaacaggt tcctttatta cctgaaaaga
    10501 tcgaaaagcg ttcatttgtg agacactcct tttaaaattt tcttttatca taccataaag
    10561 cctactcttt tattcttgtt cttccgtaaa tttagacaaa aattaggtga aaaataaaga
    10621 atcaaacaac tagaatttat tccaaaagat aaagaaatat tcagcaaaaa aaattttaca
    10681 aatggttgcc tttttcaata aatttcgtat actaatacga gtggtagtaa acaggaagtt
    10741 tactattaga aaacttcgat tcggaggaaa ttatggaaat tggtgagaag ttaagaaatt
    10801 tgcgcatcca aaagaatttg acacaagaag aactaggcga acgcacagat ttgtcaaaag
    10861 ggtatatttc ccagttggaa cgagatttaa gttcaccatc aatggaaaca ttcttttcta
    10921 ttcttgaagt cttaggtgtg actccagaag aattttttca tcaagaagca gcgaatctgc
    10981 aagtggttta ttcaaaagaa gatcatacgg tttatttcga tgaagaaaac gggtatgaat
    11041 tggaatggct cgtggctgat tcgaatgaaa aagaaatgga gcctgtcttg ctgacttttg
    11101 aaggtgcagg agaatataag acgtttgaac cttcattatc tgaaacgttt atttttgtat
    11161 tagaagggga attagagtta agcttaggag agacggtcta tgtggcaaaa gcaggacagt
    11221 cgatctatta tcaagcgaca aaacaacatc aattaaggaa tcatctcaaa aaacgaacaa
    11281 aagtattgat tgttgcaacg caatcatatt tgtaatatag ggggtaaaaa cgtggggaaa
    11341 ccaatcattt catttgatca agtagtgaaa cgctatgatg atgaaacagt attaaaaaaa
    11401 gtaagttttg agatcgaaca aggaaaattt tatacactgc ttggtccttc cggctgtggt
    11461 aaaacaacga tcctacgtat tatagctgga ttcaccgaag ctacagaagg ggacatctat
    11521 tttgaaggga aacggttgaa tgatttgcca gctaataagc gacaagtaaa tacagtcttc
    11581 caagattatg cactttttcc tcatatgaat gtttttgata acgttgcttt tggattgaag
    11641 atcaagaaaa tgtccaaatc agatatcgaa aagaaggtaa aagatgcttt gcgtatggtc
    11701 cagcttcctg gatacgaaaa acgggagatt agtgaaatgt ctggtggtca aagacagcgt
    11761 gtagcgattg cacgagcaat tgtaaatgaa ccgaaagtat tgctattaga tgaaccgtta
    11821 tcggcgcttg atttgaaact acgtacagat atgcagtatg aactgcggga tctgcaacaa
    11881 cgattaggaa tcacgtttat ttttgttact catgaccaag aagaagcgtt ggcaatgagt
    11941 gatgagattt ttgtcatgaa taaagggaaa atcgtccaaa gtggtacacc cgtagatatt
    12001 tatgatgagc caatcaatca tttcgttgct gattttgttg gggagagcaa tatcgtggac
    12061 ggtgtaatga tcgaagataa tctagttgag tttgttggta agcaatttga atgtgtcgat
    12121 ggcgggatgc gtctaaatga accagttgaa gttgttttac gcccagagga tttgacgatc
    12181 acgacaccag ataaaggaaa actagtcgta acggtcgata ctcagctgtt tcgtggtgtc
    12241 cattatgaaa tcatctgtta tgatgaacag caaaatgaat ggatggtcca ttcaacgaaa
    12301 aaagctaaag aaggaagcaa agtaggattg gcctttgagc cagaagatat ccatgtcatg
    12361 cgtttcaacg agtctgaaga agaattcgat gcacgtcttg acagctacga agaatagggg
    12421 ggacaagaat gaaaacaatg cgcaggatct attccgtccc ttatattttg tggctcggtt
    12481 tattcgtgat cgcgcctgta ttaatgatca tttatcaatc tttttttgat atgaatgggc
    12541 aattcacgct gaataattat ctatcttatt ttacgtcggg gacatcgctt tctatgacga
    12601 tcaattctgt atggtatgca tttttgatca cgtttttcac gctgatcatc agttacccga
    12661 ctgcttattt tttaacaaaa ctgaagcaca agcaattatg gctgatgctg attattttgc
    12721 ctacatgggt gaatctgctg cttaaagctt acgcatttat tgggattttc agtgtgcacg
    12781 gcagcgtgaa tcatttcttt gattttatcg ggatcgggcc gaaacagata ttattcacag
    12841 acttcagttt tctgttcgtg gcaacgtata tcgagattcc atttatgatt atgccaattt
    12901 tcaatgcatt ggaagagatg aatccttctt tgatcagtgc tagtagagat ttaggcgcga
    12961 atagctttga gaccttccga cgagtgattt ttccgttatc attaaatggt gtgaaaagtg
    13021 gtgtccaagc agtatttatt ccttctcttt ctttattcat gctgactcgt ctgattggcg
    13081 gaaatcgggt gatcacttta ggaacggcta tcgaacagca tttcttggtt acacaaaact
    13141 gggggatggg ttctacaatc ggtgtgattt taatcattgc tatgttcatc gtgatgctcc
    13201 tgacaggcga gaagaaaaaa ggaggtcgct cataaatgaa aaaatttaaa tggtcgtatc
    13261 tttatttagg gatcgtcttt ttattcttat atgcaccgat cttctatctg attttttatt
    13321 ccttcaatga aggaggaacg atgaacagct ttacaggatt tacttgggaa aattatgaag
    13381 ctgtttttga agatacacgt ttgatcatta tcgtattgaa tacgttcatg ctggctttct
    13441 tatctgcatt gatcgccacg atcatcggga catttggggc tatgggaatc tattacacga
    13501 aaaaacgacg tgcacggaca gcgttgttga gcatgaataa catcttatta gtctctcctg
    13561 atgttattat cggtgctagt ttcttgattt tctttacact actaggattc ggattaggtt
    13621 ttcagagtgt attgctttcc catattgctt tcagcattcc aatcgtcgtc ttgatggtct
    13681 tgccaaaatt gcaggaaatg aacgattcaa tggtcgatgc ggctagagac cttggtgcta
    13741 ataatttcca agtagtcaaa aatatcattt tgccttatct ttctccagga atcatagctg
    13801 gatattttat ggcatttacc tattctcttg atgattttgc ggttacattt ttcgtcacag
    13861 gaaatggctt tacgacgctc tcagtagaga tctattctcg tgcaagacaa ggaatcagtt
    13921 tagaaatcaa tgcgctaagt gcgttagttt tcttgttctc catgatattg gtcattggat
    13981 actactttat tagtaaagaa aaccgtccaa aacggctgaa gaaaaacaga agagtacgcg
    14041 agggggtggc acggctgcga tgaaaaaatt acaatctctg tttttaggta ttctcatcat
    14101 tattgcggtg ctgctgcttg gtgttcgtca acttgaaaaa acaagtggca tgagtggagc
    14161 aaaggtactt accatctata actggggaga ttatatcgat cctagtttga tcacgaaatt
    14221 tgaaaaagaa tatggataca aagtgaatta tgaaacattt gattctaatg aagcgatgtt
    14281 cacaaaaatc cagcaaggtg ggacaaacta cgacatcgct ataccaagtg aatatatgat
    14341 ccaaaaaatg atcaaagaaa aactagtact tccattagat cattcgaaga tcaaaggatt
    14401 aaataatatt gatccacgat ttttagactt ggattttgat ccagataatc agtattcgat
    14461 tccatatttt tggggaactt tagggatcgt atataacgat aaattctttg atgcaaatca
    14521 aattaagcat tgggatgatt tgtggaagcc ggaattaaaa gacagtttga tgctgattga
    14581 cggtgcacga gaagttatgg ggctttcttt gaatagttta ggctattctt tgaatagtaa
    14641 gaatatggca cagctgacag aagcagcaaa taaattgaat catttaacac caaatgtcaa
    14701 agcaatcgtg gcagatgaga tcaaaatgta tatgatcaat gaagaagcct ccgtggcagt
    14761 gacattttct ggcgaagctg ccgatatgat gtgggaaaat gaacatttac attatgtgat
    14821 cccaccagaa ggatccaatc tttggtttga taatatcgtt atgccaaaaa ccgtgaaaaa
    14881 taaagaaggt gcctatgatt tcatcaactt tatgctagaa ccggaaaatg cggcacaaaa
    14941 tgcagagtat attggttatt ctacaccaaa tgaagaagca aaagcgttat tgccaaaaga
    15001 catttcgtcg gacgaacaat tctatccatc tgatgacacg atctcccatc ttgaagtcta
    15061 caaagatcta ggatcgaaat acctaggcat ctataatgac ctattcttag aattcaagat
    15121 gtataggaga taatggcaaa actcagtaag aatggggact tatcaacgtt ctaaagagct
    15181 aaaaatcgtc ctttttctat ctgagttttc caatcttcta ccaaaagcgg atgaaacgcc
    15241 gctttttttg tgttatttta ttttttctac cacgtttttc taccagagaa aagtggacaa
    15301 aaccattgta aaatagggcc cgagacgggc tagtaaatct aagattgaaa aagttacaga
    15361 aggataaagt aattggaaac tatgcaaata tacttaaccc ttctgtaatc tattaataaa
    15421 actaatatat gaatccagtt caatatttgt agctatgaaa aaacataaaa atcatattag
    15481 ataaaaacta aattggtaga taaattattt tgtcagatat ctgaggcaaa tattcgacta
    15541 tttgtgtttc aaagtaaaaa ttggaataca aatatagaat agacatattg cagccgatag
    15601 tttattatgc ttttagacac attaaactgg aggaaaaaat gaaaaagagc gctaaagatc
    15661 gagtttttga tgagctgata cgactaacag ggaatatgtc ttctgtcact gccgagaaaa
    15721 tttcgaagaa tctaggtatc tctcgtcaga atgccagcca ttatttgacg agattagtgg
    15781 aagataaaaa ggtcgagaaa ttaccgggaa agcctgtttt gtggaagcct ttagatgaat
    15841 atgcggttgt agataatacg gagcaaataa acgaagcctt tcattctgta gttggtcatg
    15901 acggaagtct gagagaggtc attcaaaaat gtatagccgc tgtcaaatat ccgcctaacg
    15961 gactcagtgt attaataaat ggagccacgg gtgttggaaa aagtttttta gccacaaaaa
    16021 tttttgaata cgctatccat gagcaaatta ttgaaaaaga tgcaccattt gctatcttaa
    16081 actgtgccga ttatgcggac aatcccgaac tgctttcggc aacactgttt ggatacaaga
    16141 agggggcctt tacgggggct gaaaaggata ccgatggttt attggcaacg gccaacaacg
    16201 gctatctttt tctcgatgag gtccatcgcc tatcgaaaga aaatcaagaa aagctctttc
    16261 tatttattga tactggaaac tatcgtccag tcggtgaaaa tgtaaattgg cattctgcta
    16321 aggttcgttt tattttcgcc acgacggaaa aaggtgaaaa ttatttatta gacacctttg
    16381 atcggcgtat tcaaattagt gttatgttgc caacttttga agatcgcccc attcgagaac
    16441 gattagagct gattcaatta ttttttcaaa atgaagcgga tgttttacaa aaagatatta
    16501 ttgtttcaag agaagccttg tctttaatgt tatctcatcc ctttagtggg aatattggta
    16561 agctgaagaa tatcattaaa atcagttgtg cggatgtgta tagtcgaaca caagaagaac
    16621 cattaacgat tgggaaaaat gagatcttga ttcagttaaa tatgctggaa tccgaagtag
    16681 aaagcttgcc gattgctagt ttattgataa agaaaaatca aaagataaaa acggatcgaa
    16741 tcaaaccgtt tgattatgat tcgattttgg aaaaaattac tcagctgata aatgaaggta
    16801 atttcaatga aaatttttct tcgataaaga atgaagtaca gcatttggtg catcacattg
    16861 ataaggattc ttttttatca atgagtagaa aattgatgat tcagatgttt agaaaggtct
    16921 gggtatccgt tttaggaaaa acgtacggat tgacgacgag tggacctgtt gcagaaatta
    16981 ttagcagatc ctatgtcatt tccccaagct ataagacaga cttaaccggg ataaatcgta
    17041 cattagctgc aaatctacct cggacgactt acttatgtaa tcagttcttg gagcatttac
    17101 caccgctctc agaaaaggac gagaactttc taagactgct tattgtcgtg actttatctg
    17161 attatgtaga tgaatccatc gagctgaagg gcctgttgct tgctcacgga gatagtacgg
    17221 ctagcagcat tcaagcagta gtgaatcaat tatgcggcaa ttatgttttt gaagcgttgg
    17281 atatgccagt agagagtaat gtgtcagaga ttgtcgaatc gacaaggaat ttcgtgaaac
    17341 ggcaagtgaa tacagatagt ctagtgctga tcgtagatat gggctcttta aatcaaattt
    17401 acacacagat caagaacgat ttaaaagggg agttacttct tctgaataat ttgacgactt
    17461 ccattgcact tgatattggc ttgaagatga cgatgaatgc gccatttaaa cagattgcgg
    17521 aggatgcagc agacaagtat tcaatcaatg ttcaatactt tgaaggcttt tctcaaagta
    17581 gcaacattat catttcctgt atgtcgggtt tggggatttc ggacaaattg aaagatgttt
    17641 ttcgtcatca tttatcggat gaatccatcg aagttttcac gaaggactac agagaactac
    17701 gtgaattaat cgaccaaaat gatgaaactt attttgacaa gacaaaattg gtcatcacga
    17761 cttcggatct tccacgttcc ttttcaattc ctaatattaa tgtttatgac atcttagacg
    17821 gtgatggagc agccaatttg gagcatattt tgagtcctga aatgaagccg gcagaattta
    17881 accgcttgat tcaggagcta gtaaaattct tttcgttgga aggaattgcg gaccgtttga
    17941 actttctgaa cccaacgatc gtgatgagtg aagtggaaac agtcatttct aaatatgaaa
    18001 attactatca tttcacttta aatggccgtg taaaactaaa tctctatatg catacggcat
    18061 tgatgatgga acgtttattc ttggctcgga cagaaaaatc tgaagaaccg aattatgatt
    18121 tgaaaccaga tgaagatgaa ttttattatg tttctagaag tatttttcaa tcaatggaga
    18181 tgaaatataa ttttaaagtc aatgcttatg agctttcgct gctctatgaa ttattaggac
    18241 cgtatattca aaaataaagt gtctaaaaaa gtgtctaatc tgtttttttt aggttagaca
    18301 ctttttttaa tgtgaattaa cgtgattaaa ccaatgtttt taaaagttgg cacaggaatt
    18361 gcttatataa aagtgaacag aaagaaagga attaaccata tgaagaaaat tctaatagcg
    18421 actcacggtc acttagcttc aggctttctt agcagtattc aattactgac ggggaaggtt
    18481 caagagatta cggtgattaa tgcttatgtg gatgagtgtg attttgaaca ggagttgaat
    18541 gtatttgtaa accaggtgaa cgagcaggat cagatttttg tttttaccga tttatttggc
    18601 ggaagtgtca atcagaagat taccaaaacc tttttggaga aagagattgc tgtaactatt
    18661 ttgtctggct ttaatctgcc aattctgctc gaaatagtat tggcaaccaa ggaattaaca
    18721 aaggaggata ttcaggaact ggtaaataaa tgccaacagg aattaaagat gaccattctt
    18781 gatgaggtac atgtagtaaa tgaagaggaa gatttttttt aggaggaaga aaaatgatta
    18841 aagcagtgcg aattgatgaa cgattaattc atggacaagt agcgatgact tggacaaaga
    18901 gtctaaattt aaccgggtta gtagtagcaa gcgatgaggc ggctaccaac gatattcaaa
    18961 agatgacatt gaagatggct gcaccatcga atgtcaaagt cattattaaa acagttgatg
    19021 acgcgattca tttgttaaaa gatccgcggg cgaaggacat gcgcttgatg gttttagtcc
    19081 ctaatgtcaa agatgcggta aaagttgcga aagctttccc aacggaaatc gaattaatga
    19141 acgtggggaa tgctgggaaa atgacaccgc catcggaaga taagaaaacc ttatcaaagg
    19201 aagtgatgtt aacgacgtca gaaattcaag acttaaaaga attagttgaa ctttatccag
    19261 ataccgtttt tcagcctaca ccggcgatgg aaaaacgttc agctatctct attttgagtg
    19321 atttttagaa aaaggaagga gaaaaattat gtatgatgca tttgtagtcg ctttggccgt
    19381 ttttattgga gttgctggac atgaaatgtt cgggatgtcc atgctgagtc gtccgattgt
    19441 tgttgctccg ttagcaggag cgttgatggg ggatttgcag acggggttga ttgtcggggc
    19501 gtcactagaa gccatcttta tgggtgtcgt caatatcgga atctctagta cggcggagcc
    19561 ttctttggct gctgggctgg ctacagcgtt cgcaatcaaa atgggaggaa acgtggatgc
    19621 gattattccc attgtctttc cactagcagt attaggatta cagttaatga atatgatttt
    19681 ttcttttgta tgtggaccga tggctgctaa atttttcaaa tttgcaaaat ctggtgatca
    19741 aaaaggaatc atcaatctgc acttctttat gtggtttgtc cactatgcgc tttatgctct
    19801 tattccattc tttgccgttt tatttggagc agatgttgtc aaaagcgtct tggataatat
    19861 tccagaggtc atcatgaacg gattaacagt cgctggaaat ctgctgccgg cagttgggat
    19921 ggcgatgctg ttacggatgc tttggagtaa aaatattgct gtgtggtttt tctttggggc
    19981 cgttgttatg gcgtatctcg agttaccatt gattgcaatt gccgcagttg gaacgattat
    20041 tgcaattgtt atagcccaaa gagatctaca actaaaaaat atatctgttc atgcagttca
    20101 aagcgatgct gctgtaaatg caacgcttga ccaagaagag gaggatttct ttcaatgaaa
    20161 ttagctgatg gaatttctaa agatgagaaa aaattggttc ggaagatgtt ttggcgctcg
    20221 atgactatgt atgcttcggt gaatccggtg acgatgggcg gcggtggttt cgcctattct
    20281 atgatgccct tcattaatca tttctacaaa agtgaagaag atcgagctgc ggcgctggaa
    20341 cgtcatacac agtatttttc gacgaccatt ccttgcgcgt cctttgtcat ggggattgcg
    20401 gcatcgatgg agaaggaaaa ttctgaaaaa gctggttttg acgtggaaag tatcaacgcg
    20461 atcaaaacga gtttgatggg accattggct ggaattggcg attcattgtt ctggggagtt
    20521 tggcgtgtcg ttaccgcggg actagcgatc aacttagcca atgccggaaa tattttggca
    20581 ccaattatct ttttagttat gttcaatata cctaattact tgatgcgata ctatggcggc
    20641 gttttaggct attcattagg ctctaaatat attgaaaagc tgtatgcgaa cgggttaatt
    20701 gaaattctga caaaagctgc tagtatcttg ggtctgatca tggtaggtgc aatgacgatc
    20761 agtatggtca aattcaatac cgttttgagc ttttcaatga agggcgaaga actaatgaat
    20821 gtccaagaaa tcctcgatag tatttttaaa ggaattgtac cgttattggt aacattgagc
    20881 tgtttcaaat tactagataa aaaagtcggc gtcatcaaaa ttatttttgg aatggtcgtc
    20941 ttgggaattt tattgtcatt acttggaatc gcatagtagg aagctgcagc tactaggctg
    21001 cagttttttt agcaaggagg gaaaagatga ctattaatga agtaatttat tcacccaaac
    21061 acccattttt taatgctgag ttaaacgacg gaaatgtgac tacttcaaac gaatacagct
    21121 actatcggaa tattcctgat gtaaagggat tgaccttcga tgtaagtaat caaaagacat
    21181 tagcaaacct tgatgtttgc gggacgattc aaacgttgac cttttatcga gaaaatttac
    21241 taactgagga aaaaccaggt gtttgggtca ataaacagct ctctcaaacc aattcgttat
    21301 ccttaaaggt tgaagtaaac gggattgtct accatttgtc agaatcggat catcgtattg
    21361 aagtcgatgt gattcaagac tgtttgccta gatatattca tcattattcg acgtttaagg
    21421 tcattgtagt atcctttgct cccatactgg ctaataagcg tttaagtatg cttgttcaac
    21481 aggtttacat tgtgaatgaa acacacgaaa gccttcaagt ttcagtgaaa gatgtgcctt
    21541 cgtatcaaga gaagtattcg gatcagcaaa atgtgttgat ttctcaaaaa ggaaataagg
    21601 acacgatcgc acaacaggaa gtggcaagct tcgccattgc ttttgtggac ccaaatgctt
    21661 ttgaagaagt aatgactttt caggaatctg atatcgaaaa atggctgaag gaaacattga
    21721 actattttgc tcagctattt gggcagctgt caatgccaaa cgatagagtt gtccatctct
    21781 atcgtcgtgc actttatcaa agcttttcta gtttcggtat gaatcgacag ggacaggtcg
    21841 tcggcagcaa ttggggcagc tatcctgcta ctaatcgaat ttggaataag gatatgtatt
    21901 acgcatcatt gccattcacc atgtttgaac cggagttatg tcaaaaaacg gttctatggt
    21961 ttgatcaata tggcgttaag tttcctggtt caaaatttac aggaggtatc aatcattctc
    22021 tgagtaattc gttatccagt gcgttactgg ctagtcttta ttttgaacat actggggata
    22081 caaaattctt tgagcggtgt ccgcaggttc tactgaatgc ttcaagaatt attgaggaca
    22141 tattagctca acgtcggtca ggggaaccaa tgctttttcc atctgtttgg ttatcagacg
    22201 cctttgcact tggaaagtat cataccggca gcaatctttg tctttggaag gcctgtattg
    22261 gacttgcaga aatttttgaa gccctagaac aactgagtct tagcaagcga tacaaaaaca
    22321 ttgcctgtaa actaaaggaa tcaatttgta aacaaatgac tatcgatggt ccttttggtg
    22381 agcaattttt ggagggaatc ggtgatgagg agaaaacaac ctattcagtg aagcactatc
    22441 aaaaaccgat tcttgagcaa gggttaatct ttttaagtga tgtgattgtg gatggaaaaa
    22501 ttaatttatt gatgcatgat ggagaagaat cagacactac gttgatgcct ttttatcaat
    22561 ttttagataa taaagatcat ttatatcaga acaccatggc cttttctgct agttctttca
    22621 atccaaccta tagcgaggaa atcaaaggca tcacttgggg attagaatcc ggcgccactt
    22681 ttcctggctt tatcactgtt ttaatgagcg agctgcacaa ccctcaagct ttcgtaaccc
    22741 gattagaaga actaatttgt ttggcagatt tagacggttc gtggtggtgg tggccgtatc
    22801 gcttagaggc tcaacaagga gaagtcgtgc gagactttgg ctgcggcaaa tgcggctggg
    22861 catcgggtct ttttgtcagc ctaatgatca gtcaatattt cggattgaaa ttcaaaaaag
    22921 gagtattgca aattgatcct gttgataatt taacgttttc ttggaagaat ttgaagttcg
    22981 gtcaagcctt catttcgatt gaatgtacgc aatccgagtt gagtatttca aatttgtcag
    23041 aaaagccatt gaaaataagt gatacgaaaa aaattcttca cgtagaaaag ggaaaaacga
    23101 ttcacattca aaggagaaaa agatgagtat actaaaaaat attacccaat ttaaaaaaat
    23161 ccagagtcga gcaatcactc ccgaaaatcc aacaggcgag aaaggtaaag ctgcgatgtt
    23221 gccaagcgag ctaggaccaa gccgcaaagg tcgtggcgca attccgctga cttctggtga
    23281 agaaactgtg attgcggata ttactggaac gggaataatt cgtcatatgt ggatgacgat
    23341 tcgtgatcga acagaatttg gcagttttgt tttacgtgat ttaatcttaa gaatttattg
    23401 ggatgggtcg gatagccctg ctgttgaaag tcctttaggg gatttctttt gtaatgggtt
    23461 tggagagcgt tgcgaggtga actcgttgcc gatagtggtt aatcctacag ggggctttaa
    23521 ttcctatttc gaaatgcctt tccaaacatc cgctaaaatt acgatcgaga atttacatcc
    23581 gaaggatatg tcctcatttt tttatacgat aaattacgct ttggtggacg aattggaaga
    23641 aaatagccta tattttcacg caaattggca tcgggaacgc attaccaaat tacaggagga
    23701 ttataagtta attgatcgat taaagggtca aggctattac gtgggtactt accttgcgct
    23761 tacctgccta gaacgttatt ggtggggcga aggcgagttt aaattctaca ttgatgggga
    23821 tagtgagtat ccgaccgtaa cctcaacagg ttcagaagat tattttggcg gggcgtgggc
    23881 ctttcatgag tacgatgagt tcggacgtcc acatgcgaaa aactattcaa cagccttttt
    23941 agggtatcct taccaatcaa atagagatgc cacaagagat cgttttgaaa cgggaaaatt
    24001 gaatgccgtt catgcctttg gtgacgacgc aatgccacga catggtcttt atcgttggca
    24061 tcttcaagac cccatctgtt ttaccgaaga tttatcggtc actttacaac aaatcggcaa
    24121 cgatgatgtt cgcttatttg agcggcaaga cgatgttgct agtgtcgctt actggtattc
    24181 aacagaagct tcaggaaata ctaaacgtca tttcactgat cgagaacgct tgccgcgtta
    24241 atgaagtgtt tttcacatat tcgaagggag gaacctatga tgatatatgg ggcaattgaa
    24301 gctggaggga ctaaatttgt ctgtgcagtc ggtactgagg aattaacgat tttggaaaga
    24361 gtcagttttc ccacaacaac tccagttgaa acaatgaagc aggtcattga tttttttaaa
    24421 ccttttaagg aacaattggc aggcatcggg gtcggctgct ttggtccaat tgatattcaa
    24481 tgcaaatcaa cgagtttcgg tcatatcaca tcaacaccaa aacttgcttg gcaaaatttc
    24541 aattttgtag gaacgctaaa gcaatatttc aatattccga ttgaatggac aacagatgtc
    24601 aatgcggctt gctatggtga atatgttgct ggttctggca aagggttagc aggtgttgca
    24661 tacttcactg tgggaacagg tatcggaggt ggtgccttgt tcaatgggac tttcgtggaa
    24721 gggttcagtc atccggaaat ggggcatatg ttgatcaagc gacatccaaa ggacaatttt
    24781 tcaggaaact gtcccttcca ccaagattgt ttagagggga tggcagcagg accagcgatt
    24841 gaaaaacggt tgggtgtaaa aggccagaat ttattggctg acgattcgtt ttggcagatt
    24901 gaagcctttt accttgccca atgtgcatac aatacaacac tgatgttttc gccagataga
    24961 attatttttg gcggtggtgt aatgaaacaa gaacacatga agaaaaaagt tcaggataaa
    25021 tttgttgagc ttataaatgg atatgttgaa ataccaccaa ttgatagcta cattattacg
    25081 cccgaattag gtgataatgc cggaataatc ggcggattag cattggccag aaaagcggta
    25141 agaaataagc aaccataatt aatgaatgtt tcattgatac tcatgtcaaa agtatttaac
    25201 cgagtaatca aatgatcaaa tgaagttgga taaagcgaaa atgaattcaa ataaaaaggc
    25261 tctaagtcaa atcgtgttgt tttcattaaa tcactgaaag gaaatgcttc tgcgtttcct
    25321 tttttctttt tgttgtgcgc tgtatacctt gaggatattc actggtggcg tacacttaaa
    25381 tagtaatata ttgtgttact ttatgtctgt ggtaatagtt atagttgctt accaatgtat
    25441 gttcattttt cgaaatgttg acttcataat ccttggctta atgtttgaat ttttttggtg
    25501 atagattttt tcagccgtat aaaataaatc gtaatt
//
LOCUS       DAHUVV010000030        24378 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.030, whole genome shotgun sequence.
ACCESSION   DAHUVV010000030 DAHUVV010000000
VERSION     DAHUVV010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.030"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(46..894)
                     /locus_tag="NK767_001257"
     CDS             complement(46..894)
                     /locus_tag="NK767_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCH6694264.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1118..3058)
                     /gene="thrS"
                     /locus_tag="NK767_001258"
     CDS             complement(1118..3058)
                     /gene="thrS"
                     /locus_tag="NK767_001258"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCH6694265.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3395..3574)
                     /locus_tag="NK767_001259"
     CDS             complement(3395..3574)
                     /locus_tag="NK767_001259"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCH6694266.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3621..5444)
                     /gene="cadA"
                     /locus_tag="NK767_001260"
     CDS             complement(3621..5444)
                     /gene="cadA"
                     /locus_tag="NK767_001260"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCH6694267.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5722..8001
                     /locus_tag="NK767_001261"
     CDS             5722..8001
                     /locus_tag="NK767_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCH6694268.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8094..8741)
                     /locus_tag="NK767_001262"
     CDS             complement(8094..8741)
                     /locus_tag="NK767_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694269.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8920..9639)
                     /locus_tag="NK767_001263"
     CDS             complement(8920..9639)
                     /locus_tag="NK767_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6694270.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9770..10921)
                     /locus_tag="NK767_001264"
     CDS             complement(9770..10921)
                     /locus_tag="NK767_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCH6694271.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11138..11878)
                     /locus_tag="NK767_001265"
     CDS             complement(11138..11878)
                     /locus_tag="NK767_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCH6694272.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11882..12205)
                     /gene="rsfS"
                     /locus_tag="NK767_001266"
     CDS             complement(11882..12205)
                     /gene="rsfS"
                     /locus_tag="NK767_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCH6694273.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12262..12855)
                     /gene="yqeK"
                     /locus_tag="NK767_001267"
     CDS             complement(12262..12855)
                     /gene="yqeK"
                     /locus_tag="NK767_001267"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCH6694274.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12839..13489)
                     /locus_tag="NK767_001268"
     CDS             complement(12839..13489)
                     /locus_tag="NK767_001268"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCH6694275.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13499..13810)
                     /gene="yhbY"
                     /locus_tag="NK767_001269"
     CDS             complement(13499..13810)
                     /gene="yhbY"
                     /locus_tag="NK767_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCH6694276.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13826..14935)
                     /gene="yqeH"
                     /locus_tag="NK767_001270"
     CDS             complement(13826..14935)
                     /gene="yqeH"
                     /locus_tag="NK767_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCH6694277.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14932..15459)
                     /locus_tag="NK767_001271"
     CDS             complement(14932..15459)
                     /locus_tag="NK767_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCH6694278.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15639..16424)
                     /locus_tag="NK767_001272"
     CDS             complement(15639..16424)
                     /locus_tag="NK767_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCH6694279.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16417..17286)
                     /locus_tag="NK767_001273"
     CDS             complement(16417..17286)
                     /locus_tag="NK767_001273"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCH6694280.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17287..18666)
                     /locus_tag="NK767_001274"
     CDS             complement(17287..18666)
                     /locus_tag="NK767_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCH6694281.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18674..19093)
                     /gene="fabZ"
                     /locus_tag="NK767_001275"
     CDS             complement(18674..19093)
                     /gene="fabZ"
                     /locus_tag="NK767_001275"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCH6694282.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19118..19594)
                     /gene="accB"
                     /locus_tag="NK767_001276"
     CDS             complement(19118..19594)
                     /gene="accB"
                     /locus_tag="NK767_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCH6694283.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19597..20832)
                     /gene="fabF"
                     /locus_tag="NK767_001277"
     CDS             complement(19597..20832)
                     /gene="fabF"
                     /locus_tag="NK767_001277"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCH6694284.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20851..21588)
                     /gene="fabG"
                     /locus_tag="NK767_001278"
     CDS             complement(20851..21588)
                     /gene="fabG"
                     /locus_tag="NK767_001278"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCH6694285.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21600..22517)
                     /gene="fabD"
                     /locus_tag="NK767_001279"
     CDS             complement(21600..22517)
                     /gene="fabD"
                     /locus_tag="NK767_001279"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCH6694286.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22624..22848)
                     /locus_tag="NK767_001280"
     CDS             complement(22624..22848)
                     /locus_tag="NK767_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCH6694287.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22901..23863)
                     /locus_tag="NK767_001281"
     CDS             complement(22901..23863)
                     /locus_tag="NK767_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCH6694288.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23873..24313)
                     /locus_tag="NK767_001282"
     CDS             complement(23873..24313)
                     /locus_tag="NK767_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCH6694289.1"
                     /translation="MESDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6671 a         5427 c         4244 g         8036 t
ORIGIN      
        1 acgttttgtt attctaaaaa acttatataa aaaatagtaa ggtttttatt tcgaatccat
       61 aaaatattct tggattgctt ctcgtgtttt tttcactttt tcgctaagct tttcgttttt
      121 agccatatag aaataaaaaa tgatatcgat cgtagcaaat tgggccaaaa gtgaattagt
      181 tgccgcactt cgattcgtga tttcttttgg tctagatgtt tgtacgagta catcggctat
      241 cttgctaagc gggttatttc caaattgcgt caacgcaatg atttcgatat tcatgctttt
      301 cgccagctcg gctaatgcga ctacatctgc acttctacct gagtttgaaa caagccaaag
      361 cactttcttt ttctcattgc tgacaagttg cgggagcaag acgtggatat ctttttcaaa
      421 aatgattggc ttaccgacac gagaccattt ttgcagaata tcttctacta ctaaagaaga
      481 ggcgccaacc ccatataaaa aaacagtctc tgccgattcc aaacactcta cgactttttg
      541 gatcgtttct tcttctagaa tatctactgt ttcttttatc gctacttgtg cattgttacc
      601 aagtttgttt ttgatagagt aaaaggactc atttgcttct atatcactaa aaccttcatc
      661 gattccagtc tgtagttctg ttgaaatcgt gattttgaag tccgtcagac tttgaaaacc
      721 gatttttttg gaaaaacgta caactgtcgg cgcactcaac ccggaagctt ctgctaattc
      781 attagctgtc attgtaggga ccttctcaat attaccgata atgtagttgg ccaacttttt
      841 ttctgctgaa gaataatctt ccatcatact ttcaatacga ctaattacgc tcataagttc
      901 ctccaccctt ttaatgatag tctatgtaag tataccatat cagaccctta ttcacggtcg
      961 tatactggat aaaaaacaaa aaaacaatcc gcccaacaga atatcttatc ccatcaatcc
     1021 cacagattct attgagcatc tgtgtctttg aggaatacct gttatctatt gttcggattg
     1081 ctttctatct actacttttt tacaggaaat ttacctatta gttttctctg ctgtaatttt
     1141 tgacttctgc ttccatagca tcaataaaga tattcaaatc ttctacagct gtttctttac
     1201 ttccataacg acgtacgttc actgttgcat cttccaattc cttgtctcca acaactaatt
     1261 ggtaagggat cttttgtgtt tgagaggcac gtatcttata gcccattttt tcattacgat
     1321 catcaacttc tacacgcaac cccttcattt gcaaacgttc tttgatttca taagcataat
     1381 cgccatgtgc atcaacagaa acagggatga ttgttgcttg tataggtgcc aaccatgttg
     1441 gaaaagcgcc tttgtatact tctgttaaat aagcgacaaa acgttccatt gtagaaacaa
     1501 ttccgcggtg gataactact ggacgatgcg tattttcccc gtcttctcca acatatgtca
     1561 aatcaaagcg ctctggcaat aagaagtcta attgtatagt tgataatgtt tcttctgtac
     1621 caagagctgt tttgacttga acatctagtt ttggtccgta aaaggctgcc tcgccttctg
     1681 cttcaaagta atctagttct aattcatcca tagcggcttt cagcattgct tgggcttttt
     1741 cccacattgc gtcatcatca aaatatttat ctgtattgtt tggatcgcga tagctcaagc
     1801 ggaaacgata atctgtaata ttgaagtcag catatactgc caccatcaat tcaagcgtac
     1861 gtttaaattc atctttgatt tgatcaggac gaacaaatgt atgaccatca ttcaacgtca
     1921 tttctcgtac acgttgtaaa cctgacaaag cacctgattt ttcatagcga tgcatcattc
     1981 ctaattcagc gatacgaatt ggtaattcac gataagaatg gatcgtgttt ttgtagacca
     2041 tcatgtgatg agggcagttc attgggcgaa gaacaagcat ttcgccatct cccatatcca
     2101 ttggcgggaa catgtcttct tgataatgat cccagtgtcc agatgttttg tacaactcaa
     2161 catctcccat tattggtgta tatacatgtt ggtaacctag actaacttct ttgtctacga
     2221 tataacgttc gatcgtgcgt cggattgttg ccccttttgg aagccagaat ggcaaaccag
     2281 aacctacttc tggggaaacc atgaacaagt caagttcttt tcctagttta cgatggtctc
     2341 tttcttttgc ttcttcacgg agacggatat attctttcaa agctttcttg tcaaaaaagg
     2401 ctgtcccgta tacacgttgc atcatttgat tgtttgagtt tccacgccaa taagcacctg
     2461 ccactgataa taatttaaat acttgtatac ggccagtgga tggaacatgt ggtccacgac
     2521 acaaatcgat gaattcgcct tgttgataag cagtaatcac ttcttcttct ggcaattcac
     2581 taatcagttc tactttgtac ggatcacttg caaaaatttc taatgcttcc tgttttgaaa
     2641 gaacacggcg ttcgatcggc agattttctt tgacgatttt catcatttct gcttcgattg
     2701 ccggcaaatc ttctgcagtt acttggtttt gtccattatc tgtatcgtag taaaaccctg
     2761 aatcgatagc tggtccaacg ccaaagtgga tatttggata gaggcgacgc atggcttggg
     2821 ccatcaaatg agctgctgaa tgacggacta agccaagtgc atcttcatgg tctggtgtga
     2881 tgatctcaat acttgcatct tcctcgattg gacgagtcaa atcaacgagt tttccgttca
     2941 ctttaccagc taaagctttt tttgctaagc tttttgaaat gctttcagca attgccaatg
     3001 ttgttgttcc agactcaaat tctttgactg caccatctgg aaaagtaatt tttatcataa
     3061 tagtttcctc cttgaaatgt tttgattgat cagatcatca agctgaaaaa caaaaaaatc
     3121 ctagcatctc ttgaaaagag atactaggac gaaaagttcg tggttccacc taatgttcaa
     3181 acgcaaatgc gttcctcaag cgttgtaacg gtccgaccgc ccttgcttca acaagggttt
     3241 cgaaagtggt catcctttgg ttttctcctg gaaaagtttc agcctagctt ttcctctctt
     3301 atcgaagact ctcaaattca gttggctttc tcatcaatca attatgtgtt tatttaacca
     3361 ctaataaata gaaaaatcaa gtgggaaact aaacttattt tttccattcc ccatttacac
     3421 cgtatgttcc ttttttcatc tcattgatga tgacatggat gtgttctttt ggtgcctgcg
     3481 tgtttttatg aacagcttcc gtgatatctt tcatcatatt ttctaattgt tccggactgc
     3541 gaccttctac taattcgaca tgtacgaatg gcataatgct ctctcctctt tctgatcgtt
     3601 cttttttcaa ctcgattcta tcatactatg tttgtcttgg gtactgaatc aggaaaaatg
     3661 gctggatctt tttttgctct caaggcactc aaaatagaga ccgtatccac gacttcttgc
     3721 aaaacagctc cgaataatgc ggggattagt cccgtacttg caattaacat caaaaaaata
     3781 caaataaaga tgccaatcag tacagactgt ttcgctattc tcatcgtatc tcttgaaatc
     3841 actactgctt ttgctacacg caagagatca tctttcaaga tgaccgcatc tgcactttca
     3901 ctagcagctg tagaaccatg agcccccatt gcaataccga tatctgcagc agcaagtgct
     3961 ggtgcgtcat tcacgccgtc tcctaccatc gcaacaggtc tttgatcttt agggatactt
     4021 tctaaaatgg tcagtttatc ttgaggcaag ctacgggcat gtacagaatc taattggact
     4081 ttttctgcaa tcgtttgagc aatggcagat ccatccccgg tgatcatcat caaatttttc
     4141 acatcaagtt ttcttagctc cttgattgtc tttgctgctt ctgggcgaat ctgatccgaa
     4201 aataggatcg ttcctgcaaa agatcgatca attgttacat agattgctgt attggacgtt
     4261 tctatttctt cagctgaaac aaaagaggca cgtccaatct ttacttcatg attattgact
     4321 actgcttgga ttccttgacc ggtcacttct ttcagttgac taacaggata cagttggctg
     4381 tttttcacgt tgttcactaa cgaacgagct aagatatggc tagattcctg ttctgcacta
     4441 gctgcataca gaagtagctc ttcttttttt attccattga ctggctgaat cgcctctact
     4501 tccaatatcc ctttggtaag cgttcctgtt ttatcaaaag caatcgtttt tgtacgtgcg
     4561 agcttttcaa tcgttgttcc agtttttaca acgatcccgt ttttacttga acggctcatt
     4621 cctgcaacta aagcaatggg cgcagctaga atcagtggac aaggtgatgc cactaccagt
     4681 acctcagcaa atcgtaaagg gtcttttgag aaccaccaag aaataccacc gatcaaataa
     4741 gcaatcaacg tgaaagggac tgcataacga tcagctaaac ggacaaatcg ggcaggcttt
     4801 tcttttgatt cttcgactag tttaataatc atttgatact ggctgtctgc ggcacgtttt
     4861 tcagcgatca tttggatcga tccgtctcca tttaaagacc ctgacataat ctgatctcca
     4921 ggtttcttgg tttccgggcg ggactcacct gtcaaagaag attcatcgac cgaagattct
     4981 cctttggtca ctcgaccatc tgcgggtaca agttcgccag gcttgactag taactggtca
     5041 tcaatattta tttcatcgat tactatatcc tgcaattttc cttctttgaa tcgatgggca
     5101 attcttgggg tgttatctaa caaagactgc agttctttac ccgctcgttt attcgcgtaa
     5161 tcctctagac tatctccgcc cgtaagcatg atcaatatca tcaaactagc ccagtattct
     5221 ttgacggcta atgttgcaag gattgcggta atcgctaata catctacgcc atatcttcct
     5281 gaacgtaacg tcttgatcat ttccaaaaac atcaagaagg cgagaatccc tccagtaaat
     5341 gcgacaatcg caaatgcaag ggccggctgg tgaaaaacaa attcacttaa taaagctatc
     5401 ccaccaataa gcaaagtaag taaaaatttt ttgaaatgtg tcattgtttt ttctcctctt
     5461 ttatgtgact tagagaggtt agtaataaat ttccagctgc ctctttcatc ttaagcatag
     5521 tccttgacta gcacttttta aaacaatagg ccttaattga aaacaactat cactatcagc
     5581 gtttccaaaa ttcatcagtt ctacaaacaa aaatattcag caaggattac tttttcgatt
     5641 catcagttaa aaagtacctt ttttcgaaaa actatgctat acttttaatt cgttatttac
     5701 ggcggaagga gcaaacatgc gatgaatgag aaacaactag aacaaacgca cgtggatgaa
     5761 acgatcgaac taatccagat cgaacaaaat atgctggata caaaacaaca agaattgact
     5821 caggaaatga atcattcgac aaaagacaca gctaatcaca agattcgagc tggttccaat
     5881 gaagcttttt atgaatctgc tgttgaatat cgccaacatg agcaggaatt gcttttgaaa
     5941 taccatactc ttgaagcaca gcaaaaacgc ctgcaaacgt tgtctgtcat gaaagatagt
     6001 ccttattttg cccgtatcga ctttaaagaa gaccgtgaaa cagaaacatt atatctaggt
     6061 atcgcttctt taagagatct ggcagaagaa acaatcgtta ttgactggcg cgcaccaatt
     6121 gccaatcttt attatgaagg tgagattggt cccgcatttt atgaaactga aacagataga
     6181 atagatgtcg atctgcagtt gaaacgacaa tttaaaatcc aagaaggacg tattttatct
     6241 atggtagata cgtcggaagt aatcaatgat gactttttgc tagagatatt ggatgaatca
     6301 tcaagcaacc atatgaaaaa catcgtctct acgatacaaa aagcacaaaa tgcaatcatt
     6361 cgggacacaa caagtaaagt aatgctgatt gaaggaatcg ccggaagtgg aaagacatcc
     6421 gctttgcttc aacgtattgc ttttctgttg tatcataatc gcaagtggct cgaggctgaa
     6481 aacgttcttc tattttcacc caaccatttg ttttctgatt acatctcgac tgtattgcct
     6541 tcattaggtg aaagcggcgt gcctacacag acattcaaaa attatttggt gcagctgctg
     6601 ccagagtttt ctttactaga tgaacagcaa caagaaagtg gttttttgct aggggaaaaa
     6661 gatccgatac aagtgatgaa aagcggattg accttagtcg atcagattga tcgctatatc
     6721 caaggcatca caaactacgg tcccttgttc cgtgatatga aaataaatgg ccgtacgatc
     6781 ctatcaaagg aatcgattcg ccaatggtat aaagaaacaa atgaacaatt acctttacat
     6841 catagacttt ctctattaca aacgaagctc ctgaaaaaat taggcggtct gcaaaaagac
     6901 gaaacacgtc agcaatgggt caaagatctg gcggaagaac agctccaaga actatacgca
     6961 acagatccaa atcttgaata tacagaacaa aaggagcgtt cattacgtaa aaaactggcc
     7021 aaccaaatcg tacgtaaaag attccgtcga atccatcgag ggatcatgaa ttatcaattt
     7081 gtcaatataa cgaaacaata tctccacttt ctacaaactg tgccactttc tcttctaaca
     7141 cgacataaaa tcagcgagac caggtggcag gaacatttga caactgttcg tacacacttg
     7201 cgtcaaagag aattgcgtca agaggatgct gtactattct ttttactgat gcgccgaaca
     7261 tatccagtga cggttacaca caaagctcgc tatatcttta ttgatgaaat gcaggatttc
     7321 gcaccatcac aagtggcatt gctgcaagcc ttgttcccaa aagcaaattt gactttctgt
     7381 ggagatctaa atcaaaatgt ctttggaaat gaaacgatcg ttggatcatt ggaagacctt
     7441 ttcgtggacc gtgaagtcac tcgtttccaa ttaacgacaa gctatcgctc cacaaaagaa
     7501 atcactgatt ttgctaatca ttttattgtg aaagaacatc aagtagaaac aacagcaaga
     7561 aaaggagacc ttcctttgct catccaagga acaacagatg atatcaaatt gaattggtta
     7621 gcagatacca ttcgaacgac tcagggaaaa gcccggtatt ggcgtacagc aatcatagct
     7681 aaaacacaag aagaatgcga agctctttat gctcaactgc cagacgagtt gaaacaacaa
     7741 gtccagctga ttttaaatga gagtgacttt atgaagcgtt ccattatttt acttccagcc
     7801 tttttagcaa aaggtctgga gtttgaccga gttttcttgt gggatgtaga tgcagaacat
     7861 ttttcttctc agcaagacaa attgatcctt tacaccatgt gtacacgtgc gatgcatgaa
     7921 ttgacccttt ttacctccaa taatgaaagt cctttgttaa acaatatgga tatttcatct
     7981 tatgagaaaa aagtactgta agtaaaaaga caactgctat cattaatgtt agcagttgtc
     8041 tttttattta caataataat tgcagaaaaa gtaattcaat catttaaggt catttattct
     8101 atatataaat ccaaatctgt ttcaggatat tttaatttga caagcgaaat aatcattttc
     8161 agttcttcca tgttttctgg atattctggg tcaaatacct tgctatgacg gccaatcccg
     8221 ccattgtcga tcaatcggta aattcgattc cattcaatga attggtcaat tgattggaaa
     8281 ttgctgattg gatagccgcc acaatccaaa atctctgata cacgaaatgc taggtatgca
     8341 ctatcgatca ctttattttt tttcgtgtct atcttctcat ttctttcgat aagaattttt
     8401 tcaggacgat gtttcaagct ccactctaat agtagaaaga aagcctccat ccgatactct
     8461 tcttcaaata accgaaaggc tgaatcagaa cgacgaaaaa gcagcggaat cagataattt
     8521 tgtctataaa tcagcattgg tgcgcggtcc accatacact ctaaataacg gttcagatac
     8581 cctgtaccga catagtttcc taatcgatag ccttcaacgc cgaatcttgc gtatttttgc
     8641 gctaattttg aacaatcagt tacgttcaca gctaactctt ctactttgcg gtaaccgctt
     8701 aagatacgtt caaaaggttc taaagtgatt tttgcttcca tattcttccc cgtccatctt
     8761 ccacatactt catatcattt cacatgatac ttatgctctc atcataccac aggttgaatt
     8821 agaaaagaaa ataaatcaca caaaaaagca agacaatctg ttagtgagat tgtcttgctt
     8881 ttcgaaaaag actgaatact tttcgttaaa actggttgct tagtcttcta atgccacatt
     8941 atggaacaca tgctgtacat cttctagatc ttccagtgca tcaatcattt tttcaaatgt
     9001 ttcttgatct tctggagata gtgttacctc gttttgaggg atcatttgga tctcagcgat
     9061 tgaaaaatca gtgattcctt tttcttttaa tgcttcttgt atactatgga aatccgctac
     9121 ttcaccgtaa acgatgattt gtccttcttc ttccattaca tcgcgaacat cgatgtcttt
     9181 ttccattagg tattcaagga tctcgtcagc gtcttctcct gcaaaagcaa agattgctgt
     9241 attgtcaaac atgtaagaaa ccgctccgga aacacccatg ttcccgccat ttttcccaaa
     9301 tgctgcccgt acgtctgctg ctgttcgatt cacattgttc gtcaatgtat caacaatcac
     9361 cattgagccg cttggtccaa atccttcata gcgcaattca gagtatgttt catctcctgc
     9421 accttttgct ttttcgattg ctcggtcaat aatatgtttt ggtacattat aagttttagc
     9481 gcgatcaatg acgaaacgta gtttttggtt tgcatgcgga tcaggatcac ctgattttgc
     9541 tgccgcgtag atttcaatcc caaattttgc ataaatccgg ctattattag catctttagc
     9601 agttttcttt gctacgatat ttgcccattt acgtcccata ttttcacttc gctttctgat
     9661 ttgatagttt cataacgcaa aaacgtcctg ttccattata cttgtgaatc acgattctgt
     9721 gaagcttttt ttctttcttc cttcattctt ctcattttta gtcaattatc tatactcgga
     9781 ttgggatttg cccgaaattc tgttccggaa tattgcctcc caattgatag atttgatcac
     9841 ttctaactaa cagttccgct tcttgtgcct ctttttttcc gattcgggaa taaatagaaa
     9901 tctctttttt ttcttttaag aatcttcttc cagcatcggt aaatccaagt agtcgaagat
     9961 aatttttatt ttgttggaca ggaatctctt gctttttgat attcagcaag acataacaag
    10021 ataatcgttg gatccgtgtc catgtatatc tttttgtttt tacctttgac acaaaatcct
    10081 caaaactatc agcagttgca gctgctgttt tcatgcgcga ttctattcct tcggtcatct
    10141 gatagatatc tgtcagttcc agcaaagtag aagataacaa ccgataacgc aaatacggcc
    10201 aataagattc ccaagtctgc agcgaacaag ttgaaagatc ttcttctgtc tgaggcggca
    10261 caagtccagc tattcttttt ccttcttcta acgcatggcg aatggctgtt gcactggcta
    10321 tcctctgatc tgtgatgatt ttttcatgat agcctgcact tttacgtcgg atcggatgta
    10381 attccatagg gtggacatac ttggcatttt cctttgcata acttaatccc aagatatgat
    10441 ttggcgaaga aaaatcaagc gtaatatcgg gatacagact gcgaaaaacc tcagtcattt
    10501 tttgcggata actatacgtt tgatcagaca gttcttgaaa tgcctcttct atcctatctt
    10561 gattctcttg gacaaagcgt ccgaaactgc tgtaatcaaa cgaagtctct tcttctgttc
    10621 caaaacacaa actgtcacag tccagtgttt gcaagatttt tactgcacct ttagcaaaat
    10681 agtcagctga ctgaaccgcc cattcgacgg gaagctccac tactaaatcc acaccatttt
    10741 gtaaagcttc attcgcgcga tgccatttat cgatgatagc cggttcgcct ctttgcaaaa
    10801 aattcccgct cattacagca atgacgacct ctgctcctgt tatttttcgt gccatttcca
    10861 catgatagcg atgtccattg tgaaaaggat tgtactctac gataatcccg cagcttttca
    10921 tgcgcatcct ccttcatagt tatcgataaa aaagaaatag agacaagtaa aaaaaggcaa
    10981 aaatcggaaa aaatacccga actatataaa ctattctcgc aaacacaata cttctgcgac
    11041 gaaaagctgc actgcaagag caatgcctat atcgttcgga tatataattt atgttgaaga
    11101 tttattcttt tcttgttaac tcgagcaggc aaaaccgcta gtctttgtat gctacaaaga
    11161 accagcgctt tgatttctct gatgctgctt cgtctgtaaa atcagaaaat gcttcgactt
    11221 tcgaaaagcc gctgttttcc agcattctta aataactttc caatggatag gtacgctctt
    11281 gatgtagttc atcttcacga ataaatcgac cagacccttc ttgttcttca acgaaaaagg
    11341 taagaaaatg ttcgatgctg tgtttctcgc ttcctggata actatcccac aaaaaagcaa
    11401 aattatcccc ttgataatga tagctatatt ctggaaaaac ctcatctact tgataggtgg
    11461 agtgtacgtc aaataaaaat cggccatttt cttctaacgc ttgatagact tcatcaaaca
    11521 cttgctgtac ctcttgtcta tttttcatat agcacagcga atcagaaaaa caggtaattg
    11581 cctgatattg tccgatttct gataaatcga gcatattgcc ttccacaaat tggatcgatg
    11641 cgtcttcttc atatgctcgc tggctggcaa tcatcaacat ctcttctgaa aggtctaacc
    11701 ctgtcacttt atatccatct tttgcaaagg caaccgctaa tgctccagta ccgcatgcca
    11761 gttccaaaat attttttgtg tcttcttgta aatgacgttt cgaaaaagct aaccattgct
    11821 gataaagtgt ttcgtccatc acttcatcgt aaatgaaagc aaatgtttca taagccatat
    11881 gttaatccaa ccattcacta agatcgacta gcggagcatc tgaccatagt ttttccagat
    11941 tatagaaatt tcgttctggc gcatggaaaa catgtacgat cacatctcca agatcgatca
    12001 atacccattt tccgccttct tttccttcaa tacgttttac ttcatatttg ttttcttctt
    12061 ctttatcaat gatttcttct gtaattgcgt tgatttgacg ttcgctattt gctgaacaga
    12121 tcatgaaata atctgccaac aaggaaacat ttcgaacgtc taaagcgata atgtcttctg
    12181 cccgttttga atctgctgct tcaatcgcaa tttttaattg ttctttacta tctatgatga
    12241 tttcctccta atatgagcag cttatttttt tgctacccag taattatatg tttcgattgt
    12301 ttttggataa atctgttgtt cttgcgcaag caaatgggcc aatgtatgtt ttgtttcaaa
    12361 tgctacagct tcgtctagat cagcccatgc aatggctcgt gcatcttgta ccccaggaaa
    12421 atctcgacct ggttcaatat agtctgctac ataaatgatt ttgtcaagga gactcatctt
    12481 agcagcccca gtcgtatgca cccggattgc atgaagaatc tctgcatcgg tgattcctaa
    12541 ttcccgctct acaaacgaag cgccaacaag accatgccag attgcattat tataattcaa
    12601 taactccgga tcaaaaccgt ctcttacgat taccattttg aactcctcat ctggtcgttc
    12661 ttttgcataa tcatgtgtca atgcagcaat actagctttt tcaggagaag caccgtattt
    12721 tttggcaaga gccacggctg tttcttctac tccaagcaca tgcttgaagc gttgttcgct
    12781 cattctcatc tgtatttttt gcatcaggtt ttctctagaa taagcagtat attcattact
    12841 atagtccata ttcatacaac ccttcatttt gaatataatc aattacttta tctggaacaa
    12901 ggtagcggat agaacagcct tctttgatct tttgacgtat tttcgtggaa ctgatgtcga
    12961 tctcaggtac atctacccaa attacaggat atggagtatc cgttgtgtat cctggtcgac
    13021 gtatacccac aaagttgacc atagaagtca gctcatcgat tttataccat tttggcaaat
    13081 attctaccat atccccaccg ataataaagt aataatctgt atccggattg ttttgcgtca
    13141 attccttcat agtatcatag gtgtaacttt tcccaccacg agctaattca atcgtttcaa
    13201 tctgcaaaaa aggattgtct tccaccgcta attccaacat gttgagtcga tgttttgcat
    13261 caatcgtctg tttctcatca acatgaggtg gctgataaga aggcatcaaa aatacgcggt
    13321 caagacctaa attccggcca gcttgttcag ccatcactaa atgtgcaagg tgaacgggat
    13381 tgaatgttcc gcccaagatc cctacttgct ttttcttctc taagaattga ggcatttctt
    13441 caggaaataa ttgtgaacga acaaatgtct tagcttgcgt attcatcatg gtcactcctt
    13501 atattgcttt tacttctttt gatagccttt gatacttttc ttttgtggaa ggtttgtata
    13561 atacaataac acgtccaatg atttggacga tttcgcaatc tatttctttt tctaatactt
    13621 gagctgcttc ttctgcaatc tcatctgtat tttgaagtaa cgatactttg atcagttctc
    13681 ttttttctaa agcttcttca atttgaacga tcattgctga attgatccca ccttttccga
    13741 tttgaaaaat cggctgaaga tgatgcgctt tgctacgtaa aaaacgcttt tgttttcctc
    13801 ttaaactcat aattttcctt cttccttaaa tcaatgcttt gcgtttcaat acatctacac
    13861 cctttggcgc ccagcccgca attactactg gcgctgttac agtgatccag cctagaccgg
    13921 caaaaacgat gtctgttttt tctttcacag aaaactcaaa acgaacaagc tccggaaatg
    13981 cctcgacttc atcaggacga ggaggctgta ataatccacc tacatgtttt tgataaaatt
    14041 cgtctgcttt ttccagtttc gttcgatgaa tatttacatc atttgacaca taagcaataa
    14101 atgaagcacg attgccgctg ataaaatcaa atcttgcaag tccgcccaag aaaagtgttt
    14161 gcccttcatt cagctgataa actttaggtt tgatttcttt taacggtgca atgatccgca
    14221 aatctttttt acctaagtag tgcgccattt gatggcgatg gataattccc ggtgtatcaa
    14281 tcaaaaaatg cccgtcatct aaaggaatct cgattttatc caaggtcgtt cctggaaact
    14341 gcgaagttgt gattacatct tgtactcccg ctgtattttt gatgatttga ttgataagcg
    14401 tcgattttcc gacatttgtt actccgacga tatagacatc ccgtccatcg cggtattctt
    14461 cgattttttc taatagttcc gtcatctcat gagatttctt agcactagtt aataatacat
    14521 ctactggacg aattcctgct tcgtaagcac gttccttcat ccattgtgtt aatttagttt
    14581 ttttcaatga tttaggtaat atatccactt tgttcccaac taaaagtact ggattgtcac
    14641 cgacaaaacg gtgtagtcct gggattagtg aaccattgaa gtcaaaaata tccaccacat
    14701 tgacgatcaa tgcatctttt tgccctaatt cattcagtaa gcgcaaaaag tcgtcatccg
    14761 tcaatgagac atcttggatc tcattataat ggcgtaatct gaaacaacgc tgacaataga
    14821 cttccccgga agctaaacct ttttccaaag ccgtttgcgg cgtgtatcct aattcaccgg
    14881 gtttgtttgt ttgtataacg gcaccacagc cgatacattg taattcttct gtcattcgat
    14941 tccacctcgc cagcccatat cagggtgttt tgctaataga taggccataa ttcttttttc
    15001 ccaaaaacga ttaaattgtg tcttccaact atctgttgcg ataatgggtt ttacaaggat
    15061 gctacgtatc cctgcacgat tggctccacg tatatcagtc ataatctgat cgcctaccat
    15121 tactatctca tcatctgcta aatccaattg tttctttgct atgttgatcc ctcttgctaa
    15181 aggtttcatt gctcgtgcaa catacaaaag atcaaatttt tcaatcgctc gcgcgacacg
    15241 ttttgaatta ttgtttgaaa caacaacaac agggattcct gcatttttca tttctaaaat
    15301 ccaagtaagc aactcttctg ttccatctgg attattccat gcaatcaacg tattgtctaa
    15361 atcggtcaat actgctttga tgcctagatt ctttaattgg gcaggtgtaa tctgatagat
    15421 ggcttctacc atccatgttg gtttgtaatt tgaaaacatg ctgtctcctc atctgcaaaa
    15481 aatgaaggag cacataaagt actcctcaac cccgtattat actgcatatc agcaaaaaaa
    15541 actacctcta tactcgttcg tctttcagaa aaagcatttc tttacaaaat gaccaaaaat
    15601 aaagattgat cacagaaaaa tttagaaaca aaaaacaact aatattggcg gaacctcttg
    15661 tatcgttttt ccagtaattt atgctgatct aatcgaacaa gttccgtcaa tctttcaatc
    15721 aacgcttttt tcataatgtg tgcgacttta tatcgtggca actgctcgcc gttgaacgtt
    15781 tcaggaataa ctcgatcaac gattcctaac tcgcgtagtt ctggcgcagt aattttcatc
    15841 aactcagctg cttctgcggc gcgactaccg tctttccaca aaatcgacgc aaatccttct
    15901 ggagaaagca cagcataaat cgtttcttcc agcatccaga cttcatccgc taaacctaga
    15961 gcaagggcac cgccacttcc accttcacca atgatgatgg cgatgatcgg tacctttaaa
    16021 tcagacattt cgagcagatt tctggcaata gcctctcctt cgccgcgctc ttctgctcct
    16081 acaccgcaat atgcacctgc tgtattgatc aaggtaatca ccggtctatt gaacttttcc
    16141 gcttgtttca tcagtcgtaa agcttttcga tagccttcag gatggggaga accaaaattt
    16201 ctgcggatat tttcctgcag atcccgtcct ttttgaatac cgatcactgt taccggtttg
    16261 ccatctaatc gagcgattcc accaacaatc gcttcgtcat ctgcaaataa acgatcacca
    16321 tggcattcct cgaaagaatc gaagatctct tcaaaaaaat ccaagcttgt ccatcgttct
    16381 ttagctcttg ctaactgaac gacatcatgt gctgtcttat ccacgatcta tccatccttt
    16441 ctgtgtatgc aattcaagaa gcgtatggat tttctgtttc aattccatgc ggggaacaat
    16501 ctgatcgaca aaaccatgag ataataagaa ttcagccttt tggaaatctt ctggaagttc
    16561 ctgcttgatc gtctgttcga tgacgcgtct tccagcaaaa ccgatcaagc tttggggttc
    16621 tgctagaata atatcgcctt ccattgcaaa actggcagtt actcctccgg tcgtcgggtc
    16681 cgttaaaaca gttagataaa aaagaccttc attgctatgc cgtttgacag cagcagagat
    16741 ttttgccatc tgcattagag aaaagattcc ttcttgcatc cgtgcgccac ctgaagctgt
    16801 gaacaagacg actggaagac tttcctttgt tgctcgttcg aataatctgg tgatcttttc
    16861 tccgacaatc gttcccatac ttcccatgat aaaattagga tccatcactc caataatgaa
    16921 gggaatcccc tgaatggttg ctttaccagt gaggactgct tcatgaagtc ctgttttatc
    16981 ttgcattttt ttaatttttt ccgtatattc gggaaatgct agcggatctt tggttacaag
    17041 atccgtatcc cattcttcaa aacttttttc atcaataatc aaagctagac gctgccatgc
    17101 accgattcga aaagcgtatc cgcaatgagg gcatactttt tcttcaccga tgtctttcgt
    17161 ataaaggata tgtttgcaat ttggacactt tgcccacatg ttatcaggaa cagatggcga
    17221 gttagcaggt ttgctctctc tattaggatt gatccgaata tatttctttt tcttaaacag
    17281 agccatctat gcgcctccta tctccgtttc tggtgtccaa ttcggtaaaa attcttcttg
    17341 taaaaatgct gtgctgtaat cacctgctat gactgcagga tgactaatca aatccatctg
    17401 aaattctgcg ttcgtgatga ttccttctgt gataaattca cttaaggctc gctgcatctt
    17461 cattaatgct tcaaaacgcg tatttccatg gacgattatt tttgcaatca ttgaatcata
    17521 atatggcggg atcgtatatc ccgggtaaac tgcactgtct actcgcagtc ccatcccacc
    17581 tgctggaagc agtaagtttt ggatcttccc tggagaagga gcaaaatgga aagcaggatt
    17641 ttctgcatta atccggcatt cgattgcgtg tccttggaat actacttctt cttgacacac
    17701 attcaaaggc tcaccggctg ctatctccac ctgttttttt actaaatcga ttcctgtgac
    17761 catttctgtt actggatgtt ccacttggat tcgagtattc atttccatga aataaaaatc
    17821 gccttcttca tccatcagaa actcgatcgt tccggcattt tcataattga ctgctttagc
    17881 tgctcgaaca gcagcgtctc ctagtgcttc tctttttgtt tgtgagatca ctacagaagg
    17941 agattcttcc agtacttttt gattgttgcg ttgtaaagaa caatctcgct cgcctaaatg
    18001 aatgacatga ccaaatcgat cacctaagat ctgaacttct atatggcgag caggataaat
    18061 gattttttcc aaatacatat catcatttcc aaatgccgct gctgcttctt gctgagctga
    18121 agaaaaatgc tgtgggagct cttcttttga ttgaactttc cgaatgcctt tcccgccacc
    18181 acctgcagct gctttcagca tcactggata accaattctg tctgctattt ccaatgcttc
    18241 ttctactgta gataatacac cagtacttcc tggaatgact gggacatttg cttcttgcat
    18301 caattgacgc gcattgatct tatttcccat tgcatcgatc gttgcacttt taggaccaat
    18361 aaatgtgata ttgcactctt cacacatgga ggcaaatcgg ctattttcag ataaaaaacc
    18421 aaatcctgga tgaattgctt cggcttttgt cacgattgca gcgctcagta cttcctgaac
    18481 attcagatat gaatcaacgg cttttgccgg gccaatgcag attgcttcat ctgctaactg
    18541 tgtatgaagt gcttcggcat ctgcttctga atatatcgct acggtttgga tccctagttc
    18601 gcggcatgca cgaatgatac gtacagcgat ttcaccgcgg ttagctatca atactttaga
    18661 aaacatattt cacctatcca atcataaagg ttaattccgc ttcggctact tttttaccgt
    18721 ctactttagc gattcctttg cctattccgg ctgaagcttt cactttcaat atctcaactt
    18781 caaggtataa ggtatctccc ggtgtaactt tcttacggaa ttttgctttg tccaatccgc
    18841 cgaaataagc cgtctttccc ttgaattgtt caagagaaag taatgcaact gctcctgctt
    18901 gagccattgc ttcaacaatc aatacccctg gcataacagg ttcttctgga aaatgtcctt
    18961 gaaaaaaggg ctcattgatc gttacatttt tcttagcaac gatccgttct ccttcaacca
    19021 tttcttccac tcgatcaatc aaaagcattg gataacgatg tggaatgatt tcttttattt
    19081 cttgtatgtt cattgttttc cctctttcct gctttgctta tttttgctga atcctaaaga
    19141 gtggctgtcc atattcgaca actgactcgt tttctacaag gatttctgtg atcactccgt
    19201 cggcctcact agttatctca ttcatcagtt tcatcgcttc aataatgcag ataacttctc
    19261 ctttggcaac ttgatcacct acttttttga aagctggttt gtcgggcgct ggttgcagat
    19321 aaacgattcc cacgattggc gaaacaacct cgattccatc aaatactgcc gattcttttt
    19381 ctttcattat ttctggagca gttgttgctt gttcagatat gacattgact ggctccgaaa
    19441 ctggcaacgt ttcgtttgtt tgaaccatat tcatgcctcg acttgtttta tttttattca
    19501 tatacaattc aaattgacct tcacgcaaat cgaattccgt tagcgaggat tgatcaaatt
    19561 gactgactaa ttctctgatt tcattgatat ccaaaattat tcctcccagc gtttcatgca
    19621 cagaaccgca ttatgtccgc caaaccctaa tgaattactc attgcataag taaaacttgc
    19681 aggtttgctt tcgtttttga caactgtcaa gtcaatctca ggatcttgtt tttctacatt
    19741 gatcgtaggt gggataaatt gatgttctaa tgccagcaaa gttgccaagg cttcgatccc
    19801 gccggctgct cccaataaat gtccagtcat cgatttcgtg ctgctgacgt aagtatctct
    19861 gaaattttca cctaatgcga tttggattgc ttttgcttct gcctggtcat tggctggtgt
    19921 acttgttccg tgagcattga tatatccgac ttgttccggc tgtacaccag cttcttccat
    19981 cgcaagcttc attgctttag atgcaccaga accatctgga gtaggagctg tcatatggta
    20041 agcgtcacag tttgcaccgt atccaacgat ttctccatag attttggcac cgcgagcttg
    20101 tgcatgttct aaagattcaa gaactagaac gcctgctcct tcacccatca caaaaccatt
    20161 ccgattttcg tcaaacggaa tcgaaccacg attaggatca gttgattctg agagtgctgt
    20221 taaagctgca aatccagcaa tccccatctc acaaatcgtt gcttctgttc ctccagcaag
    20281 aatgacatca gaatagccat gtttgatgtt acggaatgct tccccaattg aattcgttcc
    20341 gctagcacaa gctgttacga tagctgtaca aatacctttt gcacctacac gcaaagcaat
    20401 atttccagct gccatgttgg caatcgccat tggtacgaac attggagaaa ctcgttttgc
    20461 acctttttca tgtagacgaa taacttggtt ttcgattgtt ggaagaccac cgatcccaga
    20521 tccgatgatg acgccaaacc ggtcttgatt cattttttct ttatctaaac cgctcatctc
    20581 cattgcttct aaagcagcgt agattccata gatcgaaaac atatccattc gtttgctatc
    20641 ttttttaata aaatatttat cgtaagggaa atctttgact tcacccgcta aggtaatccc
    20701 tgtttctgaa gcatcaaatt tagtgatcgg tgcgatgcca ttcgtcccct cttttaagct
    20761 attcaaaaag ctgtctgcgt catttccaat aggtgaagtg actccatagc cagtaattac
    20821 cactcgattc atttgtttgc tctccttttc ttatccgtgc atgaccaatc cgccatcgac
    20881 attgatgact tgtccggtga tatatggatt ttgtgctaag aaaactgctg tttgtgcaat
    20941 atcttctact tgtccgaaac gctgcatcgg aatttgcttt tccgcttgtt ccttgacttt
    21001 gtctgctaaa acttccgtca tatcagttgt gatgaatcct ggcgcaatgg cattacatgt
    21061 aatgcctcta gttgcagctt ctcgtgccac agattttgtt aatcctacta cgccagcttt
    21121 actagctgca taatttgctt gaccaatatt tccaatcaac ccagatacac tagagagatt
    21181 aatgatggcc ccttctcgct gcttcatcat ttttttcaat acgtgctgcg tcatattgaa
    21241 ggttccaatc aaattgatat ccagacattt tttgaaatct tctgcatcca ttcgcatcac
    21301 aagtttgtca tttgtgattc cggcattgtt tactaacaca tggatcgatc caagcttttc
    21361 ttctgcttct ttgatcattt gtcccgcttt ctcataatca gagatgtcac cggaaactcc
    21421 tacgcatttt acaccgaatg cttcgatttc ttctatcttt tcttttggaa tctctccgcg
    21481 accattcaaa atgatatttg ctccagcttt tgcaaaagct aaggccatcg ctttgccgat
    21541 tcctcttgta ctacctgtga taaatacatt ttttcctttt acatccataa cgttcctcct
    21601 tatagtattg ctattgtttc atcaaatgtc tgcttattct caacacgcag aactggtatt
    21661 gttttatcga tttttttcat aaatccgctt aatacttttc cggggccgac ttcgatgact
    21721 tgttcgatgc cgattgtttt taacttatgg atactctcat agaaacgaac aggttgcatg
    21781 acttgctgtt caagtagtcc cgcgattgtt tctttcttca ttatttccgt tgttgtgttg
    21841 cttatgatag gaaaagaagg ttcagagaat tgaatctgac ttaggtcctt cgccagtttt
    21901 tttgctgccg gttctaaaat agctgtatga aaaggcccgc tgacatttag agggatcatt
    21961 cttttgaacc ctttttcttt taatagtaaa acagcttcgt ctactgcttt ttcttcgcca
    22021 ccaatcacga tctgctgagg tgtattataa tttgcaggag atacgatgcc gtacttgcta
    22081 gcttcatgac agctttcctc gatcgtttca atcggcgcat tcatcactgc aaccattttc
    22141 ccgcttccag ctggggtagc ttctgtcata taagcgcctc tttttgctac taacgcaact
    22201 gcttcagtga aggatagcgc attactagct actaatgccg aatattctcc taaactcagt
    22261 cctaaagctg catctgggat gattccatgc tccttcaata agcgataata cgcgatgctg
    22321 actgttagaa tagctggctg tgtatattgc gtctgatcca aacgttcatt ttctgtaaag
    22381 caaagttcag ccatatcgta gccgagaatc tcacttgctt catcaaatgt ctgctttaca
    22441 acggcttctt catataaatc ttttcccata ccttgatatt gtgcaccttg gccactaaat
    22501 aagaatgctg ttttcatgtt tttctccaat atatccagaa tatatgtttg attcttccta
    22561 ttcaaaaagg ccgagacatt cctatttcag gaaatgtccc agcccccaac ctaattttaa
    22621 tgattatgca gtttgttttt ctacgtaagt cactaaatct tgaaccgttt tgattccgtc
    22681 ttctgtttcg attttcacgt caaattcatc ttcaatttca ttgatgattt ggaataaatc
    22741 caagctgtca gcttctaagt cttcttggat gtcagtagtc aatttgattt cttcagtttc
    22801 ttttcctaat tcttctgcga tgatttcttg aattttttcg aataccataa taatatattc
    22861 ctccgatatt gtttgctttt tatttttata atttaacagt ctacaactcc atgagaatac
    22921 ttccccatgt taaacctccg ccatagcctg taaatattac tttctgctgt tttcctaagg
    22981 tgatctttcc actagaaact gcttcatcca ataagatagg gatgcttgca gcagatgtat
    23041 tcccatattt atccatattg gtcaggaact tttcttgcgg catctttact tttcgggcaa
    23101 ttttctcaat aattctgagg tttgcctgat gcaaaagcag ataatcaact ggtttgtctt
    23161 tcatgacttc ttggatattg tttgcaacgt ctcgtacggc aaaatcaaaa atatctctgc
    23221 cagtcatttg caaataatac gaaccttctg aatcttctga atagaacgga tttttatttt
    23281 gctggtagcc agcggtcaat gagctacttc tggtaccgtc agctgctaat ttttctttca
    23341 gcaatttttt ctgtggattc gcttctaaga gaatcccacc tgctccatcg ccaaacaaca
    23401 cagcagtcgt acgatcagac caatcgatta gcttagatag tgtttcacca ccaatcacca
    23461 gtcccttagt tgagccgcag cggatcattt tttcagccat gcttaacgca taaacaaatc
    23521 ctgagcacgc agcactgata tcaaaagcga tagcatgagt tgctcccaat ttcccttgta
    23581 cttgtgctgc tacagatggt gtcgtgtaat cgggcgacat tgttgctact aaaataaaat
    23641 ccaaatcttt agggtttaga tttttctgtt ttatcaattt ttccgccact aaaatgcata
    23701 aatcagaagt ctcctgattt gttacacatc ttcgttcacg aatacccgtc cttgacgaaa
    23761 tccattcatc agaggtatcc atcatcttgg aaagatcatt attagtaata atcttttcag
    23821 gaaggacgct ggctgttgca gtaatcattc caaattgttc cattaaatat cctcacttat
    23881 agtcttgcaa gaagtcgtgc aagtttttca gtgcatccag caaggcatgc tgttcctccg
    23941 tagccattcc gtctaagatg ttcttgacca tttcacgatg aaaatgctga tggacccgaa
    24001 atagtaactt tccttttttc gttagtccca actttacaac gcggcggtca tcttcactgc
    24061 ggatacgttc gacatagcct ttttttacta acttgttaat agcagtggtc aaggtcccaa
    24121 cagtaattga tagttctttg gctacttctg aagttgtttt tttcttgtac atcccgattg
    24181 cctcgatcgt gtgcatctct gtgattgaca gatctttgaa ttgagatttt ttcagctcag
    24241 actcttcgat tgtcaaaata tcattgaaga cactaacaag ataatcattt actgttttta
    24301 aatctgattc catgttccac cgtccactaa ttagtttgat tatcaaatct tttgatgttc
    24361 aaactatatc ttaacttt
//
LOCUS       DAHUVV010000031        23944 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.031, whole genome shotgun sequence.
ACCESSION   DAHUVV010000031 DAHUVV010000000
VERSION     DAHUVV010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.031"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(1041..1706)
                     /locus_tag="NK767_001283"
     CDS             complement(1041..1706)
                     /locus_tag="NK767_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6694290.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1886..3868
                     /locus_tag="NK767_001284"
     CDS             1886..3868
                     /locus_tag="NK767_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HCH6694291.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3872..4510
                     /locus_tag="NK767_001285"
     CDS             3872..4510
                     /locus_tag="NK767_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6694292.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4538..4966
                     /locus_tag="NK767_001286"
     CDS             4538..4966
                     /locus_tag="NK767_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694293.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5423..6334
                     /locus_tag="NK767_001287"
     CDS             5423..6334
                     /locus_tag="NK767_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCH6694294.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6551..6988
                     /locus_tag="NK767_001288"
     CDS             6551..6988
                     /locus_tag="NK767_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694295.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7083..7967
                     /locus_tag="NK767_001289"
     CDS             7083..7967
                     /locus_tag="NK767_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCH6694296.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8191..11358
                     /locus_tag="NK767_001290"
     CDS             8191..11358
                     /locus_tag="NK767_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694297.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11378..11923
                     /locus_tag="NK767_001291"
     CDS             11378..11923
                     /locus_tag="NK767_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HCH6694298.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            11951..12406
                     /locus_tag="NK767_001292"
     CDS             11951..12406
                     /locus_tag="NK767_001292"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HCH6694299.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12403..13161
                     /locus_tag="NK767_001293"
     CDS             12403..13161
                     /locus_tag="NK767_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCH6694300.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13247..13987)
                     /locus_tag="NK767_001294"
     CDS             complement(13247..13987)
                     /locus_tag="NK767_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6694301.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            14048..15301
                     /locus_tag="NK767_001295"
     CDS             14048..15301
                     /locus_tag="NK767_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCH6694302.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            15623..15850
                     /locus_tag="NK767_001296"
     CDS             15623..15850
                     /locus_tag="NK767_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCH6694303.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            15973..16299
                     /locus_tag="NK767_001297"
     CDS             15973..16299
                     /locus_tag="NK767_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCH6694304.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            16367..17080
                     /locus_tag="NK767_001298"
     CDS             16367..17080
                     /locus_tag="NK767_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCH6694305.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            17390..18241
                     /locus_tag="NK767_001299"
     CDS             17390..18241
                     /locus_tag="NK767_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCH6694306.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            18362..20227
                     /locus_tag="NK767_001300"
     CDS             18362..20227
                     /locus_tag="NK767_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCH6694307.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            20241..21683
                     /locus_tag="NK767_001301"
     CDS             20241..21683
                     /locus_tag="NK767_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCH6694308.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            21791..22492
                     /locus_tag="NK767_001302"
     CDS             21791..22492
                     /locus_tag="NK767_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCH6694309.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            22664..23143
                     /locus_tag="NK767_001303"
                     /pseudo
     CDS             22664..23143
                     /locus_tag="NK767_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            23181..23660
                     /locus_tag="NK767_001304"
     CDS             23181..23660
                     /locus_tag="NK767_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HCH6694310.1"
                     /translation="MFLDSGVVLKETELERIEYTDFGLGAIETEGLNLFVYENNERYC
                     GKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKLSCIIPKGQE
                     RYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDSFTNPAIYR"
BASE COUNT         8061 a         3911 c         4945 g         7027 t
ORIGIN      
        1 acatcaaggt tcctagcctt tttataataa aatgaagcga aaataatttc ttttaaagag
       61 ccgagattat cagctatata agagtgaatt tgatgcaaaa aacagagccg aaacagtctt
      121 ggtgttcgta gctctgctta tatgtttcga atttaagaca aagagttggc aaaagatgtt
      181 gataaggaag ataagataac tgtgttatat aaactaggta actaactgta acaagctaat
      241 tttaagagat tcttattaga agacgtttag gaatataact aagaaaatga ttatacaggt
      301 aattgtcatt tcaataatgt tttctaattt tcgcgtcttt gttttaagaa aaaggccaga
      361 atcagttgga aaaaagttcg tacgatagtg aagaggggca aaatttccta ctaatattcc
      421 cactaacaag agataaaacg gccaggttgg tgtgattcct ctataaaaaa atgcccatgc
      481 taccgaaatc ccatagatta acaactgaac aatttttaca atgaaaaaat tgttctgcca
      541 ttactcaaag tctcttcctc cttgtatcgt ttatttgttt cattttatca ttatgatagg
      601 cgcttgaaca atccattttg tattttattg agtactcttg gataaactac cagcgattat
      661 catatttatt tatgttactg taagccatca tataaaagta aatgagcaga tcatagaaat
      721 ataaaaaaga actaaaattg atctaaatta tgtgcagctt gactacgtac caatctatat
      781 tacttactag attggtcgtt aatctattcg gatcgaatga gcaaaatatt tttgtgaatg
      841 tccatccttg attatgattc caaaaagaat cattgggagg ctgtggcgta aatattatac
      901 tataagccag aaatacctat cattctgaac ttttttattt aggaactttc atctcaaggc
      961 tttttcaatt aatttgtacc cctgtttttt ggataagatc atgtgcattg attttttcta
     1021 tctctacgtt catctcctac ttaatgccta tctggctcct gagggcttct ttttaaggcg
     1081 tcgtcatagt cttggctgag gatttcttgg taatctgagg gaatggcttc ccaagtgagc
     1141 aggcgaacgt tcatgacgtg ttttcctttg tgtttgatta cagcatggtt tttctctttt
     1201 tctacctctc cagcgtgaaa agacagccat gtaccatggc caaagatatc attacatacc
     1261 caacgttcga tggaattgtc cacgtatcct acagggagtg tggtatagcc gatttgtggt
     1321 ttcaaaaaag gattgaatcg atcgatcgtt tcatttttgt attgataatt gataagaaaa
     1381 agatagacca aacaaaagac gagcagaaag aaagatagtg tctttttgag tgagaagggt
     1441 tgttttgatg aataagattt cttgttaaca agaggttcat ctggcaatgt tggttctatt
     1501 gattcggtag ctgattcaat agaaggaaaa tcaatcggca gacctaaagt atgatgtaaa
     1561 agtgacagca tttcaaagtc tggcattgta cgagaatttt cccaactaga aatcgtttta
     1621 tcggaaacac caattttttc gcctaattct ttttgcgtaa gcttacaatt tctgcggtaa
     1681 gttcgtattt tttctccggt gttcatcttc ttctcctttc gattctacag tatatagatt
     1741 tattctacat gacctataca cttgatagat acgttttttt gatgaaaaac ggtatgattt
     1801 tatcaaagaa tcatttaagt aagggagagt ggtgataatc aaaaaaaagt tcacagtttt
     1861 actcacaatc atagtgaccg gtatcatgag tggttttgcc ttggttagta taaataaaca
     1921 ggtactctat cggacaaatg aatttttcca tcaaacagat acttatactc cgattcgatt
     1981 gccacaacat tttattgggc aagaagaata tgagcagcta atcatggtat ttgaaaatgt
     2041 ctcatcagaa actgagcttt cttatatgaa aaaaacagta aatgaatcca atgaagtaga
     2101 tacatcttgg aacttcaaga tacagccaaa gcttgaaatg acattgtatt atcaagggga
     2161 tgtcaaatct tctcacgaga aggtacccta ttctaatacc tcctttcgcg taacgtccat
     2221 tgaagaatcg atcaaaataa aagattttga aggtgaattt ttcttgatga cgagagaaaa
     2281 agaagcctat gaacgggtca tttcactatt cactgatcga ttcaatcaag catttcacac
     2341 tgattatgcc actaatattt ttgaagactt tgaaaatagc tattcgtatt cggttatttc
     2401 aaatacagac gtatataatt atcgagtgta tttaactatt ggattgattc ttttattcct
     2461 tttgttatca tcctttttct tattgatgaa taaagaaatc agtttattgt tgaataatga
     2521 cttttcatta catcaagtat tgtggtgttt attggggaaa aagaaaatca ttattacatt
     2581 cataattgca ggaatcattg gtggttttat ttttatgtat tataaggagc aatttcttta
     2641 tttgttccag atgtacgttt tcttgttcag cggaatatat ggtatctctt tgatttctct
     2701 tttattagtt gaaaaaatcg cttcgttaag agaagcgttc aactatatcc aatacattct
     2761 ttttacattt ggattgttgt tttctcttct tttgaccgtt gccaatgtcg aattagcaac
     2821 tatcgttttt tcttcttcac agttagctgt ttcccatgaa ccaccaaaag aaatcgctgg
     2881 tgaggactat catgtatttt atccagtaac catcgggaaa aaccacgtgg attttattta
     2941 ttccaatcgt tttgcatcag cagcagatca ggaattatat gaaactttga ataaaaacgg
     3001 aagtatatta gtcaatgtat cagattactt gaatgaagaa aatgaacagt ttggcagagg
     3061 gataaaaata aatctgaatt atcttaagaa atttcctttg attgatgtct ctggaaggtt
     3121 gatccaaata accgaaaaag aaacgcaccc tgttatattg atccctgaac gatatagaca
     3181 tacagattta aaaaatgacc tggcgcaaat aacagaatat tatcaagaaa tcagtgaaaa
     3241 agaaccgaaa tttctattta taaaaaacaa tcaacccatt tatacattta ttccttctat
     3301 cccctggatc gaacattatc cggttgttga aatactgaca ttgaaaaaca gtatgtattt
     3361 ggaaagaaat attctcagcg gagaaatcta tccaccgtta aagatcaaga taggctcttt
     3421 gtcgaaagaa aggttatttg agctgattga agaaagtcag ttacgtgata acttgcagtt
     3481 atcatttccg tatactcaga ctgaagagat agctgtcaaa gtgttaagca gatcatttca
     3541 ttacttgata cagatatttt tactcacttt cttttctttc ttcttgttga gctatgtcat
     3601 gttatgcata tattttgtct acaattgtcg gaaaatcgct atctttcgtt caagtggtta
     3661 ttcgctcttt gaaacgtata aagatttctt catgatgaac ctcatcaaat ggggaacgac
     3721 aagtgtaatc ttcctttttc ttatagaaag agagcctaaa tatcttttta atattttttt
     3781 ctttagcttc attgatttta ttctttcagt tgtttttatt ttgtctacag aaaagaaaag
     3841 tcagttattg ctgatgaatg gaggttaaca attggagccg ttgatagtga taaaaggtgc
     3901 gaaaaaaatc attcgaaaca aaacgattct tcatttggat cgtttcactg cggatgctgg
     3961 ggatcttgtg actgttgttg gagaaagtgg agcaggtaaa agtacgttat tgaatatatt
     4021 aggattgaat gatcagttca gttcaggaca attctatttg ttcgatcaag agacgaaaca
     4081 attaactggg agagagaagc gcttgataaa aagaaatacc atatcttttc tttttcaaga
     4141 ttttgggttg attgaatatg aaacaatcga ttttaattta gatattggat tgaagttttc
     4201 gaatttaaat gtttcagata agcgaacagc aaagtatgag gcattgaaaa aagtaaatat
     4261 aaacaagaag ctaagtacgc ctatctacac attatctggt ggtgagaagc aaagaatcgc
     4321 ctgtgcgaga gtcttattga agaatagcaa aatcatttta gccgatgaac cgactgggtc
     4381 attggataaa agaaataagg ataaagtagt tgaaatctta tcgcaacaag cttcccaagg
     4441 tacgttagtt atcgtagtca cgcatgatcc atatctgacc aaaataggaa acaagaagat
     4501 catgctttaa aaatcaaaaa ggaaaggatg tcgttttatg aaaaaagcag ttattatttg
     4561 tgcactaggg ataggttttg ttggaggaaa cgctatgtca tctgaagtgg aagcaaattc
     4621 agttcacggt gaaatctatt acaaatcttt aaagccagga gagattcatt ttgatgctaa
     4681 aggtaatatt attaagtcaa atttaccagt agaccgggaa atttctagaa cagcaattaa
     4741 tcatagctct ggaagatggg tttattatag tgatacaact tgtacgagaa aaacaggtca
     4801 ttctaaccac tatagtcgtg tctataatcg tcacggtgca aaagcgaaag ttggaaactc
     4861 tattcgatca gcaaatgcaa caaaaggagt atgggcctat gcaagtgcga caggaaatag
     4921 aactgacaca ttctcatgtt ggtacaatcc aaccggttgg tattaagtat ccaaaagttt
     4981 atataaaata gaaaaaacat ataatgaaac agcgttaaaa gttatttttg gtagttttaa
     5041 tatattcatt tttagttgta taaaaatata tcataataaa aacaacaaaa aaatgtctgg
     5101 aaaatcgaac ataaaagtgt ctgatttgaa tatcccagca gcggaagaag aaaaacaaaa
     5161 tcgataacat ttgttctaag aattagttac gttaaatacg gaacgcaaga tagacttgaa
     5221 aaacggtaac aaaagaagta gtatattaag gtgtgatgtg ctacttgaca tttattctgc
     5281 tgagcgaact ttctggattg tgtagcctta tatctattca ggacgtatcg agcagattag
     5341 taaaggtgtt cttgatgatt ttcatcttga acacctttgt ttttagcaat tcgcaaaaaa
     5401 cgaaaagtag gagaattgat aaatgaaaaa aagattttta gcacttgcca tcgtactagg
     5461 cactggctta ttgtcaggct gtacgaatgc cggtgaaaaa acagccgtta gttataaagg
     5521 cggtacaatc agcgaacaag aagtaatgga tagcttgaaa aaaatgcaag gagcagattc
     5581 cgctgtgcaa cagttgatcg tttatcaagt atttgaagat aaatatggag atgacgtatc
     5641 aaccaaagaa atcgattctc aatatgatca aacaaagaaa caattgggtg attcatttga
     5701 ttcacaattg aaatctgcag gttacacaga acaaacattc aaagacagta tcaaacaaag
     5761 tctagccttt caagaaggtc taaagaaaca tatcaaattg accgatgaag atttaaaaac
     5821 ggcttgggaa tctttccatc cagaagtgga agctcaaatt atccaagtag caagtgaaga
     5881 tgatgcaaaa gatgtgaaga aagctgctga taagggagac gatttctcta aattagccaa
     5941 agacaagtca actgatacta cgacaaaaga agatggcgga aaagtgaaat ttgattccac
     6001 tactacaacg gttccggcag aagtcaaaga agcagccttt aagttgaaag atggtcaagt
     6061 ttctgatgtg atcacttcta ccaatgcttc aacttatact acggaatatt atgtggttaa
     6121 aatggtgaaa aaccaaaaca aaggcaacga catggataaa tacaaaaaag aattgaaaga
     6181 aattgcaaca gacacaaaat taagtgacag taccttccag aataaagtaa ttggcgaagt
     6241 attgaaagac gctaatgtaa aaatcaaaga caaagacttt gaaaatgttt tgagtacatt
     6301 cacttcagat tctagtacag caagtagtaa ataagcggta taaataaaaa atatgaacta
     6361 tttaaatcaa taaaactgtt catctaaatc tttttattag aactaaatca acagtagttt
     6421 aattactact tgatttagtt ctttttattt taaaaatttt ttttacttag aatccagcga
     6481 ccctagatat ttttctgtat aatcttaaat aaagaaaaca atatcaaaaa atgcacgtag
     6541 aagtagataa atggagtggt tttgcaaatt tcttttattt ctagaagtta ttcacgtata
     6601 cggatttgtt tttctcacaa ataaaaaggc aggaagtaca atggatcatt tgtatataaa
     6661 atatattcat caattttccg ttttggtgtt aggcacacta attatagcgg ggattgtcgc
     6721 atttattatt caaggccgtg acatattaac agtacatcaa gagtttttca gttatgcatt
     6781 tggtattcct ctgatcgcta taaggtctga taacaaaaag tggaagaaac gctattacat
     6841 tctcttgcta tttttgtgtt tttatctgcc acctaagatt tttactttat taggtataca
     6901 atgcctatca ttttctttta tttcctatat cattgtttta attttcctca gtttcatagt
     6961 atggatgttt tatcttgtca aaaaataaat gaatgtttca tgaacagtac ttcatcacag
     7021 cctaattttt ttctttatgc tatcgttata ttaaagaaat caatcagaag ggaaagaaag
     7081 tgatgaaaaa agttttatta gtggtaaacc ccagttcagg cggtgaacaa gcaaaggaat
     7141 ttgagcaatt agcgatagca aaattggaat cggtgtttga tgaagtggtc gtcttgcata
     7201 caaaaaaagc aggggatgca aaaaacttta ctcgcgaagc ggctacggaa gggtatcata
     7261 gtgtgtttgt catgggcgga gatggaacag tcaacgaagg aatcagtgga atcgctgaac
     7321 aagaacatcg gccaaatttc ggatttttcc cattgggtac ggtgaatgac ttggctcgag
     7381 cattagggat accgctagaa cctgaggaag cgatcaatca tttttcgatt gaatcagtca
     7441 agccattgga tattggaaaa ataaatgacg actattttat gaatgtcgtc gcaattggct
     7501 ctatccctga atcaatcaat gatgtagatg cggagaagaa gacgaaattt ggaaaactag
     7561 cttatttcat gtcaggaatc aaacagttag cttccacaca atcctactcg tttcacgtag
     7621 aagttgatgg aaagaaggaa gaaattgaga gcagtacctt gctgatagga ctaactaact
     7681 cagtcggcgg ttttgaaaca ttacttccaa atgcaaaagt ggatgatggt aagctccatt
     7741 tggtttattt aaaagattct tctttgatcg atacgcttaa aacattgcca gacctattaa
     7801 aaggagtaga tgaatctacg aaaaatctag cctaccttac ttgtgagcaa ttagttgtgt
     7861 cattagctga tgaggaagaa ttgacgacga atgttgatgg agatgaaggg gataaattac
     7921 ctgtgacaat caaagtattg ccctcacatt tacaggttta ttgttaaaat ggaggttgtg
     7981 accgaggagt tggcgggaga acaccgcttg ttcgggataa gggactgtga caagactttt
     8041 gtcacagcct tttttttgta caaaaaagaa atttacacct aatagaaaaa tttttccata
     8101 aaaatacata ttttaaatat gaaagtacaa ttggaaatag attaaaacga taaaataaaa
     8161 ttgtcaaaac gtcagagagg atggtggaga atgaatcaat gtgtaaaaaa gctgattgcg
     8221 tcgattttgt ttgtcttccc tctcttgatg gtgacatccg tttttgccga tgtagagaaa
     8281 acaaacgaat caacgcaatc agagaatcaa agttttgaca agactacttt ttcagaagaa
     8341 tcgattactt ctgaaactac agaatctcaa agtgaggctt ctactaataa aaggcaagaa
     8401 gaaaataaga caagtgagac ctcagaagta gctgaacaaa cgtctgatag cagtgaagcg
     8461 gcactggcaa gagctgtttc agcggatggg tacagcgaag ctgccacaac agaagaactg
     8521 gcgcagcttc tcgcagacga atcagtaact aagatccgac tgattcagcc tttgacttta
     8581 gacagggaat tagagatcaa acgagatatc gttattgatt ttggtggatt cgctcacaat
     8641 ttcggcacac accatattta tatcaatgag atacctcagt cgatcgagtt ccagaatttt
     8701 aaaggaacag ctgcacatcc aggtggattg acaccaaata tcgatggaaa tgccattatc
     8761 attgcttata tgaccgattt ttggggaagc agatatcatt ttacgggaga aataaaattt
     8821 accgggacac ttgatttgta tgatgggtcc aaattaggac tgatttacgc tcctcgtgca
     8881 actgtcacat tagatggtgt ttccggtgtg ttggatgtcc aaccagtgaa ggaaggactg
     8941 aatgccgctc cgggtaaagc ctatttttgt agaagctatc aattgaatgt gatcaatggt
     9001 agtcaacttt atggaccgta tctagggaaa ttctacggtt ttctagattc aggagatgag
     9061 actggtagtg gaaaaacagc gccaggaatc catatcttaa gcggttcaaa ggtctctctt
     9121 gattatgatc gggcagatgg cgttagcgat gaaggagagg cagtcgatac gctacctggt
     9181 aatgtcatgt ttgaagtcag tggttcggga agcgaatttt cggtaaatac aaagatcaat
     9241 attaccaatg acaacaatcg cggaatcatc caaatgagag gttcaggatc gaaagtcact
     9301 gtatcgaatg gtgggaaaat caccatcaat acagcaacta cgggcggatt tcgcttgcaa
     9361 ggagattcta gtcaaatcca tgtatcaagt ggtggacaaa tctcggtcaa aatggctgga
     9421 gatgacgatc aacccgggaa caatgggatg cgttttgtag gaaaaggcct ttctttgatc
     9481 gtagaaggag ccggatcagt tatcgacatc gataagcaga gtgggcgttc cagcgcagtt
     9541 cgttttgaga atggtacgca aaagctgaca gtaaccaatg gaggctcatt gagggtaaga
     9601 aatacaggtg atggcaaatc ttacgtgaac agactcaatc aaggaaatca agcgatccaa
     9661 ttttatgacg cttctggttt tctagaaagt ccaggttcag ctgattttac agtcactgga
     9721 gaaaaatcaa atatcgatat acgcgcagat tccggagcaa ctgtagatac aactggagat
     9781 attgatttaa attttacggc aggtgaaaaa acttatttag tcatggtcgg gaaaacaggg
     9841 gaagataaga ccggcatcat cagtggggat gtgttgaatg tggacttaga atctcctacc
     9901 tattttgatt tccaaaacaa acggattgga agcgagacca atggcatagg cggctgggta
     9961 ttccaaggaa atggagattc ttctttaaag atcgcccatt cagaaatcgc tttatggcga
    10021 aaaacaggcg caacattcga taactttgaa aaagacccgg attatttttc tgatatgaca
    10081 gacttatcga tcaatgggac cgacttaggc aatttcgtat caagccctag tgcaggttta
    10141 acaaatgaat ttgctcagac aggtgccgga atgaaaaatt ataatcgaat cagtgccaat
    10201 aatcagctcc cgatgattga atccttaaga gtgccaacga atgcagataa aaaaatctat
    10261 gggcatgtcg ttgttcccga aggtgtcgag gcaaagccaa gagatgcttg gacaggcgaa
    10321 gtagaagtga ctttacgtat tacttacgca gatacgacga aatcatctgt agaaattact
    10381 gctctcacgc aaggacaaac agatgaaaat gaaggggcag gatataaccc atggggagaa
    10441 ggcgaacaag gcggcttgtt cgaagttcaa gtacccaatg atgagagatt aaaaacggag
    10501 gatcaagtgc aagttgttgc tgctaaaaaa gtaagaggtg gccaagaagt gacagaactg
    10561 gactctgcca aaacgacagt cgatgtgatt cctcctgaac ctgctaattt aagtaccaca
    10621 ttgatcaatg cagcttctcg cacgatcaca ggtacaggtg aagcaggagc cttggtttca
    10681 ttgaaaaaaa atcaagctgt tttgtcagaa acaactattg atgatacagg ggaatttgtt
    10741 ctttctatcc cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac
    10801 caagcaggag aagctactgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt
    10861 gggaatcaca atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct
    10921 gctacgaaga tcgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgatttt
    10981 ggtcagatca aagcaactgg tttgacacaa gaagcatttg gccaaatgga tatacaggaa
    11041 aaattatgga tttatgatca gcgagaaacg aaaaatccgt ggatcttaac gataaaatta
    11101 tctcagcctt tcacaaatga tgcaggattt tctatgcagg atgtcctata tttcaagaaa
    11161 ggagaggagt atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca
    11221 acgaatgtgc aagcagatta ttcagaatat ttaaataacg gtcgggcgtt taaacttgtc
    11281 ttaaccaaag aaaatcagat agctggtaat tactcagcag agataacatg gacattagca
    11341 gatgcgccga cagaataaag atcaaggagg atgattgatg aatcgaatat ggaataaaag
    11401 tcatcgaaac aactgctctt tacttatgat ccttctcctt ggattcatcc tttggcagac
    11461 acctgtccat gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat
    11521 tgataagaac aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc
    11581 ctacacggtc tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc
    11641 tacgacgaat tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac
    11701 attaagttat gatatgaaaa acttagcaaa aacagtggga gaaataactg ttcctcctca
    11761 aagtgcggtc actgagacac tttcactgaa agcgccagaa aaagagctgg ctggtgtggt
    11821 cgcaggaggg atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg
    11881 tgtcgtcaat cagttttctc aagtgaaagc aatcttgatt tgaaatagcg aaaaagagtc
    11941 ttccccagat ttgaaattaa aaaaagctta tgcggatcaa agcgatggaa gaaatgtgat
    12001 taaggcgaat cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga
    12061 aatctacaaa aaagaaacaa aagagcctta cctttcaata aaaaaagaaa aaatagatgt
    12121 agcacccaat acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg
    12181 acattatcaa gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga
    12241 ttttgaaata actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa
    12301 tcagccagaa acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt
    12361 atttgctgga ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca
    12421 ttaagtaccg ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat
    12481 aagagctcag ccactacaga aggaacgatt tctgtgacca aaccagatgc tgcaaatcct
    12541 gaagacgttg ttccagttcc tcctgttgat ccaaatgatc ctgatactcc ttttacacct
    12601 gacgaaccaa atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat
    12661 tttggaacaa tcaagttagg tacagcaaaa acagtatatg ctgcattaca aagcggaacg
    12721 gacagtactg gagcacaaac acaagtacag aatttcgtac aagtaacgga taaaagcgga
    12781 aattatgccg gttggaaatt atcagtcaaa cgaacagaat ttattggtac aacatctgat
    12841 acgaataaac taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca
    12901 actaatagca gtggaatgcc atctaccgtt tctggaactg gcgctgaagg aatcgaaatc
    12961 ccagcagatc aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg
    13021 atttattcat taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct
    13081 gtatccaatt atgctgtaga caactacgtt tcaacattca catggtcgtt aacagatgct
    13141 cctacagaag aggaagcgta aaatcatatc aacgacttca gggagtggag ccgattcaag
    13201 gcccactccc tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag
    13261 ttttttggaa gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta
    13321 aggagtcacc gttgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg
    13381 ataagttgat acagtaagat tttaaaagta aaaagtaagc atacttttct aactctgtct
    13441 tcaattgttt taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt
    13501 ggtttttcaa acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac
    13561 ttccaccttt ttgatcaata aagattttat cgacatttac gatctgctgt tctttccaat
    13621 tgatctgttc tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc
    13681 caatcggaag gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa
    13741 aaacaatatt actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga
    13801 gtgattgctt caagtcgata tcgataaaga aaacatcgtg attatcaata gtgcccaact
    13861 ggatcaactg attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat
    13921 tgaccaattc tctgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact
    13981 ttttcatatg caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag
    14041 gaataagatg gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg
    14101 tatctattta ttcttttcga aacaactgct agtaaaaaac agctttggtt gtatagcttt
    14161 gatggtggtg acacttttac ttcctatccc taaagtgagc tttccttacc taattggctt
    14221 attattgatg tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag
    14281 taatcttttt tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta
    14341 tttaggaaag agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt
    14401 ctatctatta gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta
    14461 tccaactgaa cactattttg gaggtatttg tattttatta agctggggcg tgatttactt
    14521 ggtattggga agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat
    14581 agtcatcagt tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga
    14641 aagacaacat caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct
    14701 agaggaattc aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga
    14761 aaataaaaaa gaaacgttgg aatttttaga aatattagaa gatcattctg cagctatttt
    14821 tgaaaaaccg caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt
    14881 tttaaaattt ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt
    14941 cgaagtgaaa acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat
    15001 ttctaacgca ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga
    15061 agaaaaagag ggatcacgtt ttagtatagc gaacaaggca aatccaacga atttgacgct
    15121 atcggagatg gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag
    15181 tgcgaaaaaa atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca
    15241 gaatcactat ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg
    15301 acttactgtc aaaggataag tttgaggaaa gtctacacgg agatagagac aacaacacag
    15361 catattcaaa agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg
    15421 tagttaaaca aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat
    15481 gatactaagt agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt
    15541 tgcttacacc agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagca
    15601 aatccctacg atcacaaaaa aaatgctgac ccagcagtta agagagttag aacaagatga
    15661 aagtatccat cgaaaagcct ataaccaagt cccgcctaaa gtagagtact cgctcaccga
    15721 agaaggaaaa tcattaagag agattctagt agcgttgtct gtttgggaag aagaacgtat
    15781 cgaaaaacaa aaagcctcaa gggaagaaga cacgctactg catacacatg gtggctacct
    15841 aaattattga ttttctatga catttatttt aaataaaaga aaacaggagt ttttttaaaa
    15901 gcatcatcta ttttttatgt aaaaaaatgg tatgattaat caagtaagaa gtgattggaa
    15961 gaaggcaagc aaatgaatag tatggaaaaa gtcaaccagt ttagagatga acgtaattgg
    16021 cgacaatttc ataacgaaaa agatttggct atctctattt ctttggaagc atctgaattg
    16081 ttagaacttt ttcaatggaa aacgccggaa gaagtaaaag aaacacaatt agaacgaatc
    16141 aaagaagaac tggcagatgt attgatctat tcatacatga tggcagacaa ccttcacctg
    16201 gatctagatg agatcattga agaaaaattg atcaaaaata atttgaaata tcctgtagaa
    16261 aaaagcgcag gtgtacgaaa aaaatacaca gagttgtaga aatgtgtcat tcggctgttg
    16321 gaaagagcaa aagaaaaaca gtaaaatgag tctggaggga gagaaaatga aaaaaaaagg
    16381 aaaagcaaaa agatggctag tcaatatcct cttgttcctc ttgttattag taggactagc
    16441 tttgattttc aatgaacaga tcaaggatta ttttgtcaga gagacaggag ataaatatgc
    16501 tatagcgaat gtaacaaaag aagatctgaa aaagaataac gataaagatg tcagttttga
    16561 ctttgatgcg gtagaaccaa tgacgacaga aggggtcatg aggtcgcaaa tgagaggaac
    16621 agatctacca gtgattgcta gtatcgcagt cccttcagtt tcaatcaatt taccggtttt
    16681 caaaggcttg gataatacgt cactacttta cggagcagga acattatctc cagatcaaga
    16741 aatgggaaaa ggaaattatg ccttagcaag ccatcgtgca acgaaccctg aattgctgtt
    16801 tacaccgcta gaaaatttag agatgggtgc caagatctat ttgacagatt tagaaaatgt
    16861 gtatacatac aaaacatttt ttaaagaaaa agttgctcct acagataccc aattactgaa
    16921 tgaagtcgag ggaaaagaaa ttgtcacact aattacttgt ggcgacatgg acgccgtcac
    16981 gcgactagtt gtacaaggag aactggaaag tgttacatcg ataaaagatg cgacagatga
    17041 tatgagatct gctttcaatc tagagacaaa aacattttag gatactggga cataagtcgt
    17101 tacagattaa aaacagccaa aggatatgaa tcgataagga aaaaccaatt tgctgaaaaa
    17161 aagagaaacg caaagaggtg catctctgat ttggttgttt tctagataga cttgtgttcc
    17221 agttttttta ttttttatat gaaaaggctt gcataaataa aaattcgatg gtatgatgat
    17281 tttgaataag aaatggattg ttactgtacg gcaggcaaaa ccagaattca gctaagaaac
    17341 agtaggcgaa ttctaggttt ttttattttt cttgagaggg cgaagcaaaa tgaaaattga
    17401 taaaattttg aataacaatg tcgtcatttc gaaaaatgga ttcggggaag aagtcgtttg
    17461 tatgggaaaa ggactagctt ttcaaaaaaa gattggggac gagatttcgc cagaagcggt
    17521 ccaaaaagaa tttgttttaa gagattcttt tgctaaaaac caattccagc agctgatggc
    17581 tgatattccg gcagaggaga tcgaatgggt caaacaagta gtcgaactgg ctgaagataa
    17641 actaaaagtt gaattttcgc ctaatattta tctgacattg accgatcatc ttcattatgc
    17701 gatcacacga gcaaaagaaa atatccaact gcctaatcca ttattatttg aaacaaaaaa
    17761 attttatccg aaagaatatc aagcagcgaa agaggctctt ttgttcgtga aacaaaagac
    17821 gggtgtggaa ctttctgagg atgaagccgg ttttattgcc tttcattttg tgaatagcca
    17881 acaaggaaac gaagacatgc aagtgacgat gaccgctacc acaatggtaa gagatatact
    17941 gagtatcatc agtaaattct tcggcatcgt gttcgatgaa gaatctttga attatcagcg
    18001 tatcattact catctgcagt tttttgccca gcgttatctc aaaggggaag gatctgacga
    18061 gcaagatgaa tttctctacg cattagttca aggaaagtat ccaaaagcgt ttcattgtgt
    18121 cgaacggatc aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga tcgcagagca
    18181 gatttattta acgattcata ttcaaagagt agtaagtgag aaaaaaagca tccaatcata
    18241 aggattgtta ctgtatggca ggcaaaacct ggattgaaaa aataggttca cgaatgtttg
    18301 tgactgtttt tttcaatctg ggtttttatt ttttaaataa aaaagaaaga aggaaaaaac
    18361 aatggataat caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac aaaagaatgt
    18421 caaaagttta gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg aatctttagc
    18481 agatacgcaa aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa gtggcggtca
    18541 gtatcaagta gtgattggca gtaatgtagc agatgtttat caatcgatcg tggatgagga
    18601 aggtcttgca gtttcggagg atggaaaaga agagtccaaa aatattttga atcgtctgat
    18661 cgatattatc tctagtatat ttacaccttt tctaggggcg atggcagccg ccggggtatt
    18721 gaaaggattc ttatcattgg caacggtgat gggctggctg acagctgata gcggtactta
    18781 tcaaatattg tttgctgcag cagatggtgt ctttaccttc ttacctgtca tgcttgcttt
    18841 tacagcagct aaaaagttca aagccaacca gtttttagca gtagcaattg ctatggcact
    18901 tgtgtatcca gctatcacag cagttgctgg tgcgggagaa gctatcagtt tctttgggat
    18961 cccagtgatt ctttcaccat ctggttatac ttcttctgtc ataccaatca ttctagccgt
    19021 atgggttcaa agtaaattag agccattcgt aaaaaaagtg attccgcaat ttctgcaaat
    19081 gattttagtt ccgcttgtcg ttttagtagt gatggttcca ttaacatttc ttgctttagg
    19141 accaatcggg acggttgccg gtaatgcttt aggcggtctc ttcaactcga tttatggatt
    19201 tagtccaatc gtagcaggtc taatcatggg aagtttatgg caagtattcg tgatgttcgg
    19261 catgcattgg ggattcgtac cgatcatgtt tttgaatatc gaacaatatg ggtttgatgt
    19321 attgatgcca atgcttcttc cagccatctt ggcacaaggt ggggctgctt tagctgtagc
    19381 attgcgaaca aaagatacga aacttcgagc attagggatt tcttctacag tgacttcgtt
    19441 atttggaatc actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga aaccattcat
    19501 tgccgcttgt atttctggag gtattggtgg agctattatc ggattttccg gagtaaaagc
    19561 tttctccagc agtttggtca gcttattgac gattccgact ttcattaata cggtggatgg
    19621 tgtagagtca aatgtgacag tagcagtaat agctacagga attgcttttg ttcttgcatt
    19681 cgtaggtaca ttgattttag gatttgacga acaaacacaa gacaatcaat tagaaaacaa
    19741 acatgcgaat gcaggagaac cgattacttc agcacggcat accttaaaaa gtcctttaac
    19801 gggtaaagta ctgccattga gtgaagtacc agaccaagtt ttttcttctg gcgtgatggg
    19861 aaaaggtatc gctattgatc cagaagtagg agaactagtt gcacctgcag acggagagat
    19921 cacgactatt tttccaactg gacatgctgt agggatcaca acgactgacg gagcggagat
    19981 tttgattcat atcgggatgg atacagtaga attaaatggg aatggctttg agatcttagt
    20041 caaacaaggg gatctcgtca aagcaggaga tttattgatt cgttttgaca tcgaagcaat
    20101 tagagcagct ggctatagcg taatcactcc agttgtcatt acgaatactg atgcatttgc
    20161 cgatatactt gaattagacc aaaaagagat tattgcaaac gaagatgttc tggcaattgt
    20221 aaaataagga ggaaatcagg atgcaatcac gtttttcaga aaaattttta tggggtggag
    20281 caactgctgc caatcaatta gaaggggctt atgatcgaga cggaaaaggg ctatccgttg
    20341 cagatgctat gcctggaggt aagcagcgct ttgcgattat cggcagtgaa gaatttgact
    20401 ggacgattga tcaagaaaaa tatatttacc cgaatcatcg aggaattgat cattacgatc
    20461 gtttcaaaga agatcttgcc ttgtttgcgg aaatgggatt caaatgttat cgtttttcga
    20521 ttgcctggac acgaattttt ccaaatggag acgaaggtac tcctaacgaa gcgggattag
    20581 aattttatga tcagctgatt gatgagtgct taaaatatga tattgaacct gtgatcacga
    20641 tttctcatta cgaaatgcct cttcatttgg caaaagaata cggtggctgg aaaaatcgaa
    20701 aactgatcga cttctatgaa cgatttgctc agacagtatt agagagatac agcagtaaag
    20761 tcaaatattg gatgactttc aatgaaatca attcggcctt tcactttcca gctttaagtc
    20821 aaggattagt aaaaagcaat ggagcaggag agtaccaaaa tattttccaa gcatggcata
    20881 atcaattcgt tgcaagtagc aaagccgtaa aaatcggcca cgaattgcgt tcggatatcc
    20941 aaatcggctg catgattatc tatgcaacaa cttacagcat cgatgccaat ccagtcaatc
    21001 aggcagcaac aatgatccaa aaccaagaat tcaatttttt ctgcacggat gtccaagtac
    21061 gtggagaata ccctgcatac acagcgcgta cccataaaaa atatggagtc gatccggaaa
    21121 aattagagca aacggaaaaa gacttccagt tgctaaaaga atatccagtg gactacatcg
    21181 gatttagtta ttatatgtct acggcaatca atgagactga tccacaagca gcaacttcag
    21241 aagggaactt gctgggagga gtaaaaaatc cgtttcttga ggcaagtgaa tggggctggc
    21301 agatcgatcc agaagggctg agaatcgcat tgaatgaatt atacaatcgt taccagaaac
    21361 cattattcat tgttgaaaac ggtcttggag ccattgatca tatggaagag gatggcacga
    21421 ttattgatga ttatcggatc gattatttac gtcgtcatat cgaagcgatg gctaatgcag
    21481 tagcagacgg tgtagatttg atgggctata cgccttgggg atgtatggat cttgtcagtg
    21541 catcaacagg agaaatgagt aaacgttacg gctttatcta tgtggatcta gacgatgaag
    21601 gaaatgggac actgaatcga tcaaagaaaa aatcttttga ttggtataaa gaagtaattg
    21661 cctcaaatgg cgtaaacttg taattaaagc agctgaatga aaaagaacac acttctcaca
    21721 tgtgatttgt gttctttttt tattttttat gtgctattct tttagtaata gtaaagcaaa
    21781 gggtgacgtt atgaaaagga cacgtgtact atacttggaa gtcgcagata aaattaaaga
    21841 agatattttc tcaggaaaat atccagtcgg ttcgatgtta ccgacagaaa gtgaattaga
    21901 agaactgttt caagtcagca aaatcactgt ccgaaaggca atcgaactgc tggcatcaga
    21961 tgaatatgtc gcaaagaaaa gtggtcgtgg gacgacggtt ctaagcaatc gtccttacaa
    22021 tcgattatcc aaagcggcaa gctttacgca aatcttggag aactcccgat atgaagtccg
    22081 gaaagaaacg ctggatttgc aattggtcca tataaagaag gaagatcctt tgttttcctt
    22141 tttcggtgag caagctgttt gttttaagcg cttatattat ttgaacaatc agccttatat
    22201 ttattttgtt cattatcttc cacaagaaat gatgcaattc agcagagaag aatttggcaa
    22261 agaatctttg tatcgtttgc tgacgaaaaa aggatatact attgactcat tttctgatga
    22321 tttctcggca gtatttttaa cagaagaaga acaagaaatt ctgaaaacag acgcacagat
    22381 cggcatcaaa cggatacgga aatcgtctac gcaaattggg gaaatgattg aatattcgga
    22441 ggcggtctac catacggagc tgcatcctta ccatattgat tatgaaacat aaataaaaaa
    22501 agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagctt
    22561 ctcatattag cttctcatat ttttcgtttg gttaggctta ttaccgcaga tacccggctg
    22621 tttctggatt accatttatt ctggataagg gggtgtggca cgattttcag aaggaatcta
    22681 tgaaccaatc actgtattga ttttagcttc gatggtaacc ccattcggct tgacgatcgc
    22741 ttatttctta gggaaaatca ttcgtaaaaa tatattcaat cgtcaagaaa tcgatacatt
    22801 gaagaccgct tttcctatgg ggatctgtca gatcacagag ggatgtttcc ctatcgtatt
    22861 gaacgacttg gcacgcaatg tcatagcaac gggagtcggt ggggcagtcg gcggtggatt
    22921 gagcatgttt tggggcgcag atagccgtat tccggcatcg ggaatgtttg cagtcgctac
    22981 gatgaccaga ccatgggcat tcattggtgc attgttagct ggttcttttg ctacgggaat
    23041 aactatttta ttattgaaaa aaccagttga cccaaatgca gaagtcatcc aagaagaaaa
    23101 agaggaagaa gagatatctt gggatgactt gacaatcagt taggaggaac agaatggatt
    23161 acaagaaaag agtacaacaa atgttcctag attcaggagt agtattgaaa gaaacagaat
    23221 tagaaagaat cgaatataca gattttggat taggagcaat cgagactgaa ggattgaatt
    23281 tatttgttta tgaaaataat gagcgatact gtgggaaaga actggtatta ttgccttatc
    23341 aaacttgtcc agagcatcgg cacccacctc gagatgagaa tgaaggaaaa aaggaaacct
    23401 ttcgttgtcg aaaaggctta gtatatcttt atgtcgaagg aaaagaaaca aaaaaacttt
    23461 cctgtattat tccaaagggg caagaacgtt actatacggc aaaaaaagaa atcatacttc
    23521 acgctggaga tcagtacacg atcgaacaaa atattaaaca ctggttccag gcaggtgcaa
    23581 caggcgcggt tatcaccgaa ttttcttcca gcagtgatga tccaagtgat agttttacga
    23641 atccagctat ttatcgttaa tacaaaggtt tgggaacaag ctgatgaacc aaaatgatca
    23701 aatttcgatt atttggttcg tcggcttttt tatttagaga aacatcaggc gttggtgaaa
    23761 cataacacga gaataaaatg gatttttcat taataggaga aacaagtgtg tgactattta
    23821 aataatgcgt gttattgaca tgcaggacga aatcccaatt tcttctataa gtaagtcgtt
    23881 tcaattttat atttactagt tatttatttt tttggttact gtaaaatagc tgaaaaatta
    23941 tttt
//
LOCUS       DAHUVV010000032        23517 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.032, whole genome shotgun sequence.
ACCESSION   DAHUVV010000032 DAHUVV010000000
VERSION     DAHUVV010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.032"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            131..505
                     /locus_tag="NK767_001305"
     CDS             131..505
                     /locus_tag="NK767_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCH6694311.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="NK767_001306"
     CDS             complement(619..1248)
                     /locus_tag="NK767_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCH6694312.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="NK767_001307"
     tRNA            complement(1382..1454)
                     /locus_tag="NK767_001307"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="NK767_001308"
     tRNA            1668..1741
                     /locus_tag="NK767_001308"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="NK767_001309"
     CDS             complement(1879..2043)
                     /locus_tag="NK767_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694313.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="NK767_001310"
     CDS             2338..3666
                     /locus_tag="NK767_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HCH6694314.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="NK767_001311"
     CDS             3868..4701
                     /locus_tag="NK767_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCH6694315.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="NK767_001312"
     CDS             4717..5454
                     /locus_tag="NK767_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HCH6694316.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="NK767_001313"
     CDS             complement(5609..6577)
                     /locus_tag="NK767_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCH6694317.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="NK767_001314"
     CDS             6809..7642
                     /locus_tag="NK767_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCH6694318.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="NK767_001315"
     CDS             7676..8515
                     /locus_tag="NK767_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCH6694319.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="NK767_001316"
     CDS             8541..9101
                     /locus_tag="NK767_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCH6694320.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="NK767_001317"
     CDS             9300..11021
                     /locus_tag="NK767_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCH6694321.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="NK767_001318"
     CDS             11186..12328
                     /locus_tag="NK767_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCH6694322.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="NK767_001319"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="NK767_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCH6694323.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="NK767_001320"
     CDS             14128..14775
                     /locus_tag="NK767_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCH6694324.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    14904..15120
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15168..17813
                     /locus_tag="NK767_001321"
     CDS             15168..17813
                     /locus_tag="NK767_001321"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCH6694325.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FYRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="NK767_001322"
     CDS             18123..19436
                     /locus_tag="NK767_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCH6694326.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="NK767_001323"
     CDS             19426..20079
                     /locus_tag="NK767_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCH6694327.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20826
                     /gene="radC"
                     /locus_tag="NK767_001324"
     CDS             20134..20826
                     /gene="radC"
                     /locus_tag="NK767_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCH6694328.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20976..21176
                     /locus_tag="NK767_001325"
     CDS             20976..21176
                     /locus_tag="NK767_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCH6694329.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21430..23109)
                     /locus_tag="NK767_001326"
     CDS             complement(21430..23109)
                     /locus_tag="NK767_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCH6694330.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23111..23323)
                     /locus_tag="NK767_001327"
     CDS             complement(23111..23323)
                     /locus_tag="NK767_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCH6694331.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
BASE COUNT         7802 a         4016 c         4881 g         6818 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 tttatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttattccatc gcagcaggat
    20701 atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac tgtactggac
    20761 cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat gatggaagag
    20821 aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt gtaattttgt
    20881 ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa tgttacaatt
    20941 taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca gtaaaatggt
    21001 tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac gtattcgttc
    21061 acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa gcagtgactt
    21121 tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa gcttaatttt
    21181 atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag cgagtaacaa
    21241 aaatacctgc cacaagagac aatttctctt gtggcaggta tttttttgta tgaaaaagta
    21301 aataaattta agaggctatg aaagagccac cttgtatcaa ggaataagaa caaacaaaaa
    21361 tggcttctca tgtttttcat ttgttttctc ttatattcag gacaaggtgc tgatttcata
    21421 acctcttatt tattcttcat ctggtgtcat taccattggc aaaatcattg gatgtctttc
    21481 tgtacgttcg aataagaaag gctgaagcgc gccagtcatc gcttctttca atttatattc
    21541 tgaacagtca ggtccattca aacattctct taaagcgtgg aataagattc gctgagcttc
    21601 attgatcatt tctcctgatt ctcgcatgta gacaaatcca cgagagagaa tatctggacc
    21661 agccaagatt tcttttttct tgatatccac tgtcgcgaca gctaatacaa gaccttcttc
    21721 tgaaagcagg tgacggtcac gtaaaacgac attaccaata tcaccgatcc cgctaccatc
    21781 gatatacaca tctccagcca caaaatgccc agcaggacgc gcagaatcgc tagttaacgc
    21841 taaaacatcg ccattttcca tgatgaagca gttttctgca ggaacaccta catcttgtgc
    21901 tagagaagca tgtactttca acatgcggaa ttctccgtgg acaggaataa aatattttgg
    21961 cttcatcaaa cgaagcatta atttttgttc ttcttgtcca ccgtgaccgg aagtatggat
    22021 gttattgatt tttccgtgaa tcacttctgc accagcttca gacagcaaat tgatcaaatg
    22081 attgacactt gttgtattac caggaatagg tgaacttgaa aagatcactg tatcgtctgg
    22141 ctggatttgg atttgacggt gagtaccatt cgcgatgcgg ctcaacgcgg ccatcgcttc
    22201 tccttgagaa cctgtacata ggatcatcgt ttcattggca ggcaagcgat tgatttcgtg
    22261 tgcatctaca aatgtgccgt ctggaacttt gatatatcct aaacgttgac cgttgacgat
    22321 ggcattttcc atgcttcgtc cgaaaacagc gattttacga ccagttgcca cagcggcatc
    22381 tgctgcttgc tgcaaacgga agatgttaga agcaaagctg gcaaaaatga tacgtccttc
    22441 gatttttgaa aagatcttca aaatagaatg cccaatcgtt ttttctgatt tagtaaatgt
    22501 tggaatctca gcatttgtac tatcagataa aaggcacaag actccttctt ccccgatacg
    22561 agccatttta tgcaagtttg ctggttcgcc aacaggagtg aaatcgaatt tgaagtctcc
    22621 agtcgctacg atatttccag aaggtgtttt caccacgatc cctaatgcat ctgggatact
    22681 atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa cggatgatcg tatcttcatt
    22741 gatctcatgg agctctgcat ctcgaagcaa gccatgctca tctaatttgt ttgtgatcaa
    22801 cgctaatgcg agaggaccag catagatagg aatatttgct tgtttcagca agtagggtat
    22861 tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag gcttttactt tgtgtaagtt
    22921 ttgtatgatg tagctgtagt cgggaatgac atagtcaatt ccaagtaaat cgtcttccgg
    22981 gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg aattgtaccc catacgtatt
    23041 tttcccgatt tcacctaatc caccaatagc aaagacgcct gtctcgttat ttttgatgga
    23101 aagtttcata ttaaaactcc actaatgaga aatcagcatt ttctttttcg tactctagat
    23161 gacttccttc tagaagttga acgaattcaa tattgtatgg ggtattttct tccacacgtt
    23221 ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga ttttgtttct tcccgtttag
    23281 ggttgcgtac ttttgtttct tgataataaa ctttatagat cattgttttg tctcctttat
    23341 ctgcgtaaag cagtgattta gctagttgat ttttttagac aacacaaaac gaggggacaa
    23401 gccacctggt ttttcatatg ctttgactag aatactggat tcgagtcaaa gagacaagcc
    23461 tcgtttttgc ttatctgttc ggatattttt tcaaccgtac cttctgtata aaaaata
//
LOCUS       DAHUVV010000033        23097 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.033, whole genome shotgun sequence.
ACCESSION   DAHUVV010000033 DAHUVV010000000
VERSION     DAHUVV010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.033"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            241..795
                     /locus_tag="NK767_001328"
     CDS             241..795
                     /locus_tag="NK767_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCH6694332.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(968..1441)
                     /locus_tag="NK767_001329"
     CDS             complement(968..1441)
                     /locus_tag="NK767_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCH6694333.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1453..1989
                     /locus_tag="NK767_001330"
     CDS             1453..1989
                     /locus_tag="NK767_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCH6694334.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2029..2400
                     /locus_tag="NK767_001331"
     CDS             2029..2400
                     /locus_tag="NK767_001331"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCH6694335.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2684..3955
                     /locus_tag="NK767_001332"
     CDS             2684..3955
                     /locus_tag="NK767_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCH6694336.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4200..5225
                     /locus_tag="NK767_001333"
     CDS             4200..5225
                     /locus_tag="NK767_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6694337.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5519..6628
                     /locus_tag="NK767_001334"
     CDS             5519..6628
                     /locus_tag="NK767_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCH6694338.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6711..7607
                     /locus_tag="NK767_001335"
                     /pseudo
     CDS             6711..7607
                     /locus_tag="NK767_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7623..8447
                     /locus_tag="NK767_001336"
     CDS             7623..8447
                     /locus_tag="NK767_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6694339.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8479..9789
                     /locus_tag="NK767_001337"
     CDS             8479..9789
                     /locus_tag="NK767_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HCH6694340.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9937..11196
                     /locus_tag="NK767_001338"
     CDS             9937..11196
                     /locus_tag="NK767_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCH6694341.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11206..12078
                     /locus_tag="NK767_001339"
     CDS             11206..12078
                     /locus_tag="NK767_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6694342.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12090..12920
                     /locus_tag="NK767_001340"
     CDS             12090..12920
                     /locus_tag="NK767_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6694343.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12960..15143
                     /locus_tag="NK767_001341"
     CDS             12960..15143
                     /locus_tag="NK767_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCH6694344.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15204..16424
                     /locus_tag="NK767_001342"
     CDS             15204..16424
                     /locus_tag="NK767_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HCH6694345.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16421..17881
                     /gene="gtfA"
                     /locus_tag="NK767_001343"
     CDS             16421..17881
                     /gene="gtfA"
                     /locus_tag="NK767_001343"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCH6694346.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17958..19064
                     /locus_tag="NK767_001344"
                     /pseudo
     CDS             17958..19064
                     /locus_tag="NK767_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19386..19595
                     /locus_tag="NK767_001345"
     CDS             19386..19595
                     /locus_tag="NK767_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCH6694347.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19588..19737
                     /locus_tag="NK767_001346"
     CDS             19588..19737
                     /locus_tag="NK767_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCH6694348.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19891..20556
                     /locus_tag="NK767_001347"
     CDS             19891..20556
                     /locus_tag="NK767_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6694349.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20840..21925)
                     /gene="xerS"
                     /locus_tag="NK767_001348"
     CDS             complement(20840..21925)
                     /gene="xerS"
                     /locus_tag="NK767_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HCH6694350.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22259..22492
                     /locus_tag="NK767_001349"
     CDS             22259..22492
                     /locus_tag="NK767_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HCH6694351.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22489..22896
                     /locus_tag="NK767_001350"
     CDS             22489..22896
                     /locus_tag="NK767_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCH6694352.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT         7901 a         3455 c         4411 g         7330 t
ORIGIN      
        1 aacttctacg gataactgcc cgtctaaaat ttaaataaga gcttagaatc ttcatataag
       61 taaggttaat ttttttgtac aaaaaagtcg gaatattctg agttactgaa atgatataaa
      121 atcataaact aaatcccttg attttattga ctttcaaaag tttatatata taagttaatg
      181 attatggtaa aatatataca taatcattaa ctcagaatat ttttaagaag ggaaacaata
      241 gtgagctata atgtacaacc gttaaggaca aatgaagaga tacaagattt tcagttttgg
      301 ctaagaagaa caagtaatcc ggaaagagat agttttttat ttttgtttgg aatcaataat
      361 gggttacgta tgtcagatat tataggatta aaagttggag atatacgagg caaaagtaag
      421 cctattattg ttgaacataa aacaggtaag cgaaaaccga tttttataga taatttgaga
      481 gaagaaattt tattatatac tgaaggtaaa gaagaaaatg attggttatt tccgagtcgg
      541 caacaaggtc gacatataac aagggatcgt gtctatcaga tatatgcaga tatagcagaa
      601 aaattaggac gagatgatat aggaacacat acattaagaa aaacttttgg gtatcactac
      661 tacaagaaaa ctagagacat tgctacctta atgtttattt ttaatcatag ctcgcaagca
      721 attacaaaaa ggtacattgg tataactgaa gatgaaatag gagcatcact tcgtggattt
      781 aaactaggtg tataatttaa aaatttttat aaacagtaaa gacaaagatt aaaagctatt
      841 tttatctaga acgattttat ttcataaagt tcgcggaata aaagctgggg ttactggtac
      901 ttggtatagg ttcgtgttat ataaactaag taattaaata acatttttag gttggaaaag
      961 gatgaaatta aaaaaagaga atcaatacgg tctcaaaaac gcaaacgctg cggaataaga
     1021 aaaatactga taactcaacg tttagcagac tatctaatag ggatagtctg cttttttcgt
     1081 ttttgaccat acttttgacc ttctgcaaac ttccttgctt ttcctctgta gaactcatga
     1141 aatctgcaaa acgaattgcc gtttcctctg tctgcttttt cgcgatatgg gtgtatatat
     1201 catccatagt ggttttaata tctgtatgtc caagtcgaac atacacttct ttgacagacg
     1261 caccagattc aaagagcaga ctacaatggg tatgacgaaa accatgaagt gtgatatgtt
     1321 ttaagttatt ctctttaatg atgattttca aattgtgatt tacatagtct aaataaaggg
     1381 gcttattttc ttctgatgaa aacaaaagtt gttcaccatc ttttataggt ttgattctca
     1441 tttttagata ttatgcccgc acgtattcat cacatttcta ttgtgaatcg ctttattcga
     1501 ccgacatttg acttttatca taatatactc ggtttaaaat tactaatgaa aacaattaat
     1561 caagacgatc ataccatgta tcacctgttt ttttcagata atcaccaccg tgtgggaact
     1621 gaactgactt tttttgaagt acaagaaggg aacaaccagt tttttggaac caatacgatt
     1681 gaacgaacga tactaaaagt tccttccgaa gcatcattgc atttttggga aatatatttt
     1741 gaaacacaag gcgtttgcca ttatggaatc gaatcatttt ctggacgacc tatcttacgt
     1801 tttgaagggc cagatgcaac acgattagct ttagttccct tacgcgaatt tgaagatatt
     1861 gatgattatt tcccgtatgt cacagaccag atccctacag agcatgccat tttaggaatt
     1921 gatgccattc aactacgcgt acaatattcc gatgcgactg aacgagagtt attgtcggta
     1981 ggctggtagc acaaagaata cgtcagtttc ttttcgggtc atcaacgagt gacaatctta
     2041 gaaaatcaag acactcagtt ctatcaagaa gttcagatta ttcaagatcg tgagaatcca
     2101 gttgccattg aagggattgg tagcgtccat catgttgcgt ttggcgtaga aaataaatct
     2161 gatttacaac gcatcgacaa acaattacaa gagcgcaatt ttataaattc aggaataaaa
     2221 gatcgtgaat tttttgtatc actttattat cgtgacccaa atcaattatt gattgaaatt
     2281 gctacagggg aaggaaacct agatgcaaaa gcgtatgaaa atcaaagtcc acgctttgaa
     2341 gaaattcctt tatttttacc gcagtactta aatttcattc gtgaacaagt ggagcaataa
     2401 ttagctaatc aaatccatga cgaagagtaa gaggttgact cataagtcaa aaaataaaga
     2461 aaaacgcatc aaatccattc taaagaatgt tgatttgatg cggttcctat ttttataaac
     2521 tatcattttc gactaataaa actacttatg agtcagcctc tctttttttc ttgattttta
     2581 tacctctttc taggtaaact aaacatctgc caaaaatacc gtgtctcatt gaaacattct
     2641 caactatttc aaaataaagg ggagtaccaa atgaacgagt atattgaaat tatccacgca
     2701 agagaaacaa cttaaaaatg tcagtctcaa aattcccaaa aataaaatta gtatttttac
     2761 cagcgtatct ggttcaggaa aatcatcgat tgtttttgat actattaccc aagaagctgg
     2821 tcgtcaactg aatgacacct atgatagttt tacccgttta tttttaccta aatacagtcg
     2881 tccagaggtt gatgagattg ataatctttc tacggcgatt gtggtcaatc aaaagaagat
     2941 tggtggcagt gcgcgctcaa cgttagggac gattactaac atcaatgcgt tatttcgtgt
     3001 gttgttttca tgttttgcaa cacctagttt aggatatgca aatgcttatt catttaatga
     3061 tcctagtgga atgtgttctg tctgtcaggg aatcggtaag acgattgcat ttttaatagg
     3121 attgctttat ttttcggcta ttgataatgc tgattttgta ttttctgatc catttaccgc
     3181 agaagttttg tctggaattg aggaacaggc aaagaatcat ggttattttg tattggtgca
     3241 taatgttaat tcagcagaag atgttaaaac tattcagaga aattggcgtt ttgaggggtt
     3301 cattgttgtt ggtgtacgtg taaaagaatt tttggaatta aataacgttt ttgatggtcc
     3361 tgttgtttat attgatacgc atctttcaaa agagacatta agagaacttg aacaacaaat
     3421 gaatcgttta tttgtaaata caaatgatta ccaagctagt acattggcag tggaacattt
     3481 gattcattat ggtcatgaga aaatagcgtt tctttctttt gactacattg taggtgaacc
     3541 aagtgttatt caagaacgac atcaagctta tatcgataca ttaaagaaat atcatttaac
     3601 ctttaaatcg cattggatat ataacaactc tgaatttgag cgaatttatc aagaattagc
     3661 tgaatttact gcaattgttg ttactggaga ttatttagcg gctaaattag taaaattttt
     3721 aaaaaataag aataaacgag tgatggaaca attatcgatt attggatttg atgatattac
     3781 ttttgcagaa ttaatggatc ctgcgttgac aacaatacga ctaaatccag gtaacaaggg
     3841 ggcgattgct tttcaccaac tgattggact ggccaaacaa gaagaaataa aaaatcaagt
     3901 actactatta gagggagaat tgatttctcg agactctgtt cacaaaatca aataaaaaag
     3961 ctctgtgaaa taagtatagt taacaataaa atatccgaac gatagagaga atgaactgtg
     4021 atctagcagt tcctcgtcgc aaattcacta gaattagcat tatttactct aaattgttcg
     4081 gatatttttt cttgttttta attatgatta cagtgcctat ttttaacgaa atttgcttat
     4141 tttcaaaatg tatgattata tgttagtaaa cactttcaaa tatgaaaaga ggagctatta
     4201 tggtaggaat tagggatgtt gcaaaacggg cgggtgtgtc cattgcaaca gtatcgcgcg
     4261 tgctaaatga ggatccgaca ctttcagtaa ccgatgaaac caaacgaaag attgcagaat
     4321 cggttaattt ttatcattat gtcaaaaaaa gtcccgttgc aaaaaattta acaaatattg
     4381 cattaataac gacagtttct gaagtagatg agttggaaga tccctatttt cgaacgattc
     4441 gcagagggat tcaagtcgaa gcagaacaaa gcaaagtcaa tttgaagaaa attattcgtt
     4501 tgtcagaaac accgttggag caagacgatt tgctaaaatg tcaaggtgtg ttgattattg
     4561 ggcaggtgct atcttctgtg attgaagaag tatcagcaat taattcgaat gtcgtagtga
     4621 ttgatgaccc tagtattgaa gctgaagtag atgcagttta tactgattta acaaaagcaa
     4681 cgaatcagca tttagatcga ttgtataaaa aaggacaccg aaacattgcg tttatcggag
     4741 gaggacgtgt tcgtttagat agccaaggaa ataaacacgt gtcagatgat gattatcgaa
     4801 aaatagccta tgagacgtgg atgaaacaaa aaggattaga aaaatatagt caaatttatt
     4861 tagaaggctg gaccacactt gacggaatgc aggcagcaga tttacttttg aaaaaacatg
     4921 ctaaagataa gttgccgact gctatcatgg taggaaatga tcctattgct gtgggggttt
     4981 atcgttcact tcaaaaaaat ggtctacata ttccagaaga tatttcgatt gtgagttttg
     5041 ataatattga agttgctgaa tttttaacac ctgcattaaa tacggtgaat gtgaatacag
     5101 aagaaattgg aaaactagca gttcgcatgg ttaatgaacg aataaaagaa gttagaaatg
     5161 tttcgatcaa agtgattgtt tctaataata ttattgttag agaaagtgaa aaaaatatcg
     5221 attaaaaaag taaacatcca aaaaagggtg tttacttttt tgtttactaa tatttttagt
     5281 aaaaaatctc gacaaaacat taagtaaaca gttatggtgt tgttaacaaa aagaaaacgc
     5341 attcaaaaca ggaggaatta agatgaaaca tttcaaaaaa atagctttaa cagggatagc
     5401 tgttttaact gggacattac tcgcagcatg tggtggggga gatagtaatt ctaaagacgg
     5461 gcaagtaact ttaagctatg cgagttgaga tactgaccaa gctccaggac taagaaaaat
     5521 gttagatgaa tttgaaaaac aaaatcctga tatcaaagta gaaatggaga caacgccttg
     5581 ggatcaatat tgggttaaat tagaagctgc aacaacggga ggtaatatgc cagatgtagt
     5641 aaccatgcac tctagcgaat cctataaata tatgtcacag aatgctttga tgggacttga
     5701 tgatgttatc aaagaaaatg atattgacat gggaaacttt actgaaggaa ttgctgattt
     5761 ctatacgctt gaagacgaac tatacgcaat ccctaaagat gcttctgtag tggggctatg
     5821 gtacaacaaa gaattatttg ataatgcagg tgtcgattat ccagatgaaa catggacatg
     5881 ggaaacactc agacaagctg caattgattt aacagatgaa tcaaaaggca tctatggttt
     5941 tgcatcacat attggaacag agactggcta ctggccattt atttatcaaa atgggggaga
     6001 agtatttgcc gatgacaata caaaatcagg tttgaatacc ccagaagcac aagaagcttt
     6061 agctttttat cgagatattg cacttgttga tcatgcttca ccaacaatta cagaattaca
     6121 agatcctgat aagttaagtc gttttcaatc aggtagatta gcaatgatga ttgaaggaaa
     6181 ctggtttgca ccgggatttt tgaaaaatga ctatctagtt gaaaatgttg cttctgccgt
     6241 gttacctaag ggaaaagtta atgcaacaat tacaaatgga cttggttggg ctgccagtgc
     6301 gagtacaaaa tacccagaag aagtgaaaaa actaatggca tttttagcta gtgaggacgc
     6361 caataaaatc caagcggaaa cgggggcatc aatcccagca ctaaaaggct ttgaagacga
     6421 gtgggctgct tcttcagata caattgattt gagtgctttt ggaaaaatgt tggagtatgg
     6481 agtccctcgt ccatacaata aagatggatt acgagctgaa caagcagaag ttcaaattat
     6541 taaccgtatt ctgtctggtg aagtaactgt tgaagatgga accgctcaaa ttgaaaaaga
     6601 agtcaatgcg atattgagcg gaaattaaat tagttaatta tacaatatac aggcgaaagg
     6661 attctatttc gtctgtatat taatggatga ataccaaaaa gggggatatt atgaaaaatc
     6721 gaatttttgg aaaatatcgt aaagatcaag tgatatgggg ctatattttt atttttccaa
     6781 cggcattagg attaatttta ttaaatatat ggccagcaat tcaaacagca attttaagtt
     6841 ttcaaaaaac ggtagggttc ggtgatactg aatgggtcgg attaagaaat taccaaaaaa
     6901 tgtttgcaga cggtgaagtt tttcagtctt tattaaacac attaatttat gcagtggttt
     6961 cgatacctag tattgttgta ttatccttgt tggtggcagt tttaatgaat agaaaagtta
     7021 aaggattgtc gatttatcgg acaatttatt ttctaccagt cgttgctgca ccatccgcag
     7081 tcgcaatgat ttggcgctgg atgttcaata atgattatgg actgattaat aacatgttgg
     7141 ctaaaatagg tctcgatgga ccagcatggt taacggatcc atcgattgcg atttattcta
     7201 ttatcattgt aggagtttgg agtgctattg gttataacat ggtgttatta ctagccggct
     7261 tataagaaat tcctaaagat tattatgaag cagcggaaat tgatggtgct agtccaatcc
     7321 agcagttttt caacattacg ttaccattag ttacaccaac attatttttt gtagttgtaa
     7381 caacagttat caatgctttc caagtatttg atgttatttt tatgatgatt actcctaata
     7441 gtaccgccat gtttaaatca caatcattag cctatttatt ctataaacat tcatttattt
     7501 taaatgacaa gggatatggt tcggcaatcg ttatgttcct tttagtgatt atattaatta
     7561 ttactgcaat tcaaatgaag ttgcagaaaa agtgggtaaa ctattaagga ggctatcaaa
     7621 ttatgaaaaa taaatttttt tcaaatgcgc caatccatat catgcttatt ttaggtggta
     7681 ttgcaatggc attacctttt atttggatga ttttaacttc agggaaaacg ttggttgaat
     7741 ctacccaaat tccacctaca attttccctg agaaatttca gctaagtaac tatcaacaag
     7801 tacttgattt attaccattc atggcttttt atatcaatac atttttaatg gttgttggac
     7861 gagtgattgg ctcggtttta ttttcagcaa tggctgcata tgcttgtgcg cgtctaagtt
     7921 ttccagggaa aaatatcctt ttcagcttag tacttattca aatgatgatt cccggttcca
     7981 tttttattac tccacagtat ttattagcac aaaaattggg tattttaaat acaattccag
     8041 cattaatatt accaggggta gttagtgcgt ttggtacgtt tttattaaga caatttttct
     8101 taggtttacc tgatgaattg gaggaagcgg cctatttaga aggtgccaat atttggacga
     8161 tctttttcaa aattatgttg ccgcttgcta aatcaggatt ggttgcactt ggaatattca
     8221 cagcgttgtt tgcatttaaa gatttaatgt ggccattgat tgtcaatact tcaattgata
     8281 agatgccttt agctccggga ttagcaaact tacaaggaca atatgccacg aacttcccac
     8341 aattaatggc aggttcgtta ttagcaattt ggccaatagt tgtgatattc atcgttttcc
     8401 aaagaaaatt cattgaagga attgctacaa caggtagtaa attataaaac tattaattag
     8461 tgattggaga gtaagtacat gacaaaaatt acatttattg gtgcaggaag tactattttt
     8521 gcaaaaaacg tattaggcga tgcgatgtta actccaagtc tacaagatgc acatattgca
     8581 ttgtacgata ttgatgaaaa acgactaaaa gaatcaaatt taatgcttaa aaatattaat
     8641 aaaaattcga atgaaaatcg tgctgatatt gtttcttatt atgatcgcaa agaagcgtta
     8701 agagaggctg attttgttat caatgctatt caagtgggtg gttataaacc ttcaacagtg
     8761 attgattttg atattcctaa aaaatatggg ttgcgacaaa ctattggtga caccatgggg
     8821 attggtggga tttttagaac cctacggaca ctaccagtaa tgttagactt tgctaaagaa
     8881 atggaagaag tttgtccaaa tgcttggtta ttgaactata caaatccgat ggcgatgtta
     8941 accatgggtg ttttaaaagc gacgagtatt aagactgttg gtttatgtca tagcgttcaa
     9001 gtatgcgtac cagaattgtt tgagcattta ggaatagccg atcaatatga cttaaatgac
     9061 tttcaatgga aaattgcagg cattaatcat atggcattct tgttggaaat tacaaaaaat
     9121 ggggaagatt tctatccaac aattcgtcag ttagctgcag aaaaaccaaa tccacatcca
     9181 gattctgtac gttttgaatt gttgaaacgt tttggttact atattacaga atcaagtgaa
     9241 cacaatgcag aataccatcc atactttatt aaatcaaaat acccagaatt gattgacgaa
     9301 ttagggattc cgttggatga atacttacgt cgttgtgaac aacaaattaa tgattgggaa
     9361 agcatgcgag aggacattgt aaataatggg gagttaacac atacgagaag ccgtgaatat
     9421 gcttcatata tcatggattc tattacgaca ggaactccgt cagtgattgc aggaaatgtc
     9481 ttaaataaag gcttaatcac aaacttaccg acagatgttt gtgtggaagt accttgttta
     9541 gtcgataaaa atggcgtgca accaacctat gtcggtgaat tgccaacaca attagctgct
     9601 ttaaatcgga cgaatatcaa tgttcaagaa ctgacagtag aagccgcttt aacactagat
     9661 aaatcaaaaa tttatcaagc agcgtattta gatccacatc tatcatctga attgtcttta
     9721 acagatatca aaaatttagt ggatgattta atcgaagcac atggggacta tttacctgaa
     9781 tataattaaa aagaagctga aagagtaagg aattttcaat tccttactct tttcttagtt
     9841 aaatttagta aaaatattga caaaacactt agtaaacagt tacattagtg ttaacaaaaa
     9901 gaaaacgcat tcaaattgta taaggaggag cataaaatga agtggtctaa aaaaattatt
     9961 ttagcttgta tagcactagg agcaacgatc tctttgactg cttgtggaaa aaatcaggaa
    10021 gatgcaagcg gtaaggtaga aatagagttt tttaatcaaa aaaaagaaat gactcaaacg
    10081 attcaggaga ttgctaaaga ttttgaagca aaaaaccctg atattcatgt aaaagttgtg
    10141 gatgtaccga atgctggtga agtaattaaa acacgtatgc ttgcaggcga tgtaccagat
    10201 gtcattaatc tttatcctca aagtatagaa ttgaaagaat gggcgaaagc tggttattta
    10261 gaggacttaa ccgaagaacc gtatttagag aatatcaaaa atggatatgc acaacgtttt
    10321 gctattgagg atcgtgtata tagcatacca ttaaccgcta atgtctatgg tttttactat
    10381 aacaaaactg cttttgagga aatggggatt caagcaccag aaacttgggc agaatttgaa
    10441 aaaatagtag ctgagattaa agatcaaaac aaagtaccgt ttgcgattgc aggagcagaa
    10501 ggctggactt taaatggtta tcaccaacta gctatagcga ctgctgctgg tggtgaagag
    10561 gaagcgaaca atgtttggcg tttctcagaa gttaatggaa ttaatgctga aagcaaagaa
    10621 atgcaaaaag attttaatcg attagatttg ttaagagaac caaaagcttt gcaaaacaac
    10681 tggcaaggtg ccggttataa cgatacagta gttactttta caaaaggtga tgcgttgatt
    10741 atgccgaatg gttcatgggc aatgccgatg attcattcac aaaatccaga ttttgaagta
    10801 ggaacttttc cttttccagc agatgaagca ggtcaaagtc taacgattgg cgcaggagac
    10861 ctagccttat cagtttctgc aacttctcaa cataaagaag aagccaaacg ttttgtagaa
    10921 tatatgacaa cacctgaagc gatgcagaaa tattatgatg tagatggtgc accgtgtgct
    10981 gttgagggcg ttatcgagaa tactcaggat tcaccattaa gtggtttaac tgaacttgct
    11041 tttactgatc gtcatttagt ttggttagcc aaagattgga atagtgaaaa tgatttttat
    11101 accttaacaa caaattattt acacaatggc aatcaaaaag ccatgattaa cgcattaaat
    11161 gctttcttta atccaatgaa agctgacgga aggtaggaaa ataaaatgaa aataaaagaa
    11221 attttaaatc gttactgggc aatcattttt gtagcaattc caattatttt gcagatgatt
    11281 tttttctact ttccaatggt tcaaggtgct ttttacagtt taactaattg gactggtctg
    11341 acttataatt ttgattttgt gggtttgaac aattataaga ttcttttttc tgatccgaag
    11401 tttatcaaat cgattggctt cactttaatt gtgacgcttt gcatgattgc tggacaaatt
    11461 ggttttggta ttttgattgc gcgggcgttg aacgcaaata ttaaagggaa aacattcttt
    11521 agagcatggt tcttttttcc agcggtctta tcgggcgtgg ttgtttcttt agtatttaaa
    11581 caaattttta actatggttt accaaaaatt ggtagtatgt taggcattga atttttacaa
    11641 accagtcttt taggaacaac atggggagcg atttttgcag taatttttgt tttactttgg
    11701 caaggaattg ccatgccagt catcattttc ttagcgggtt ttcaaagtat tccacaagaa
    11761 attattgaag ctgctagaat ggatggggcg aatgataagc aaattttttg gaatattgaa
    11821 atcacctact tattaccaag tatttcaatg gtatttatta tggcattaaa aggtggctta
    11881 acagcatttg atcaaatttt tgctttaact ggtggtggac caagcgatgc cacaacatct
    11941 ttggggttac ttgtttataa ttatgctttt aaaaacaatc agtttggtta tgcgaatgct
    12001 attgctttag tcttatttat cttaattggg attgtttcac tggcacaatt aaaattatcg
    12061 aaaaaatttg aaatctagga ggtttcccaa tgaaaacatc aaaagaaaaa aatatttcta
    12121 aatacttgct tttattatta gggggcttca tgattttagt tcccttagtt tcaacgctat
    12181 tcggttcatt taaaacaaca cccgatatta tgaataattt ctttcgtttt cctaatccag
    12241 cgactttgga aaatttccga agattgttag cggatggaat tggtcattac ttttggaatt
    12301 cggcaatcat cacaatcatt gcagtagttg ctgtgacagc aattattcct atggctgctt
    12361 attcactagc aagaaaaatg actcgtggga aagcatacgg attgatgtat ttatttttaa
    12421 ttttaggaat ttttgttcct ttccaagtaa ttatgattcc gattaccgtt atgatgagta
    12481 aaattggttt agcaaatatg tggggattaa tcttactcta tctcacttat gccgtaccac
    12541 agacactatt tttatatgtt ggttttatta aactaaatgt gcctatcagt ctggatgagg
    12601 ctgctgaaat tgatggggct agtcacttta caacttattt caaaattgtt ttcccaatgt
    12661 taaaaccaat gcatgcgacg actattattc ttaatgctct atggttttgg aatgatttca
    12721 tgctaccatt attaatttta aatcgtgatt ctaatatgtg gacactacca ttgtttcaat
    12781 ataattatac gggacaatat tttaacgact atggtccaag ttttgcttcc tatacagtag
    12841 gaattgtcac gattacacta gtttatcttg ctttccaaag aagtattata gctggaatga
    12901 gcaatggagc agtgaaataa ccaataaatt attctcatta taaaggagtt cataaactta
    12961 tgattatatt tgacaatcac acacaaacat ttcatttaca aaataaaaaa attagctatc
    13021 tattaggaat cgaagaaaat aattatttaa cgcatcttta ttttggaaaa aagattacgc
    13081 actattctgg tgggtatcgc tacccccgaa cagatcgttc cttctcaccc aacaatgcaa
    13141 atgcgcaaga tcgtttatta tctttagaca cattaccatt agaatttcca ggatatggac
    13201 atggtgattt tagagaaccg gcgcataata ttaaattagg aaacggtagt cgtgtaaatg
    13261 attttcgtta tgatagctac gaaattcaaa aaggaaagcc cagcttagcg ggattgccag
    13321 ccttatatac ggaagatgat gatgaagctg aaacgctgat tattcaattg aaagacgcga
    13381 ttagtggatt gactttacag ttgttctaca caatttatca aaatcacagt gtaattattc
    13441 gttctacgca attagtaaat aatagtgatg aaacagtgga gattaaccgc ttagctagtc
    13501 aatcaattga ttttccaaat cgtccactcg agttaattca tttaaatggt acctgggcaa
    13561 gagaacgtca aatgactaga gaaaaaattc atacagggat aaaagtatta gatagtaaac
    13621 gcggatcaag cagccatcaa caaaatccgt ttgttgcttt agtagatgcc aaaacagatg
    13681 aatttcaagg agaggcatat ggatttaatt tagtttatag cggtaatcac gaaacggtga
    13741 ttcaaaaaga tccatttgac caaacacggg tcattatggg aattaattca tttaattttg
    13801 gttggcaact aaaaactggc gactcatttc aaacgccaga agtcgtgatg gtttatagta
    13861 atgaaggtct gaatacgtta tcacagacgt atcatgaatt gtataatcac cgtttaattc
    13921 gtggaaacta tcgtttaaaa gaacgtccca cgttaatcaa caactgggaa gcaacgtatt
    13981 ttgattttaa cgaagaaaaa atcatgaata ttgtcgatga tgcgaagaac ttgggcattg
    14041 aaatgtttgt tttagatgat ggttggtttg gcggtcgtga aaatgacttc acttcattag
    14101 gtgattgggt tgaaacagaa ggcaaacttg cttgtggttt ggaaaattta gccaagcaag
    14161 tgcatgataa cgggatgaaa tttgggattt ggtttgaacc ggaaatgatt tctgaagata
    14221 gtgaactatt tcgagcacat ccagattggg cattgggtat tccaggtcgt gaccgctcaa
    14281 ttagtcgaaa tcaatatgtt cttgattttt caagacaaga tgttcgtgat aatatctatc
    14341 aacaaatgac ggcgattttt gatcgtgtac cgattgatta tgtgaagtgg gatatgaatc
    14401 gaaatatgac agaagtctat tctgtcttgt tggactcaac gatgcaaggt gaagtggctc
    14461 atcgttatat gttagggtta tatgaatttt tagaaaagtt aacaacgaat tatccaacta
    14521 ttttatttga aagttgttcg ggtggaggcg gtcgttttga tgcaggtttc ttgtattaca
    14581 tgccacaaac ttggacgagc gacaataccg atgccgtggc tcgtctgaaa attcaatatg
    14641 gaaccagctt agtgtatcca atttcaagta tgggtgcgca tgtttcagca gtcccaaatc
    14701 atcaaacagg aagagaaacg agtttaacta ttcgtggcaa tgctgcgatg gctggtgttt
    14761 ttggttatga gctagatcta acgcaattaa cttctgaaga aaaagcagaa attaaagaac
    14821 aagttgcctt ttataaaaag catcgccaac ttttgcaata tggaactttt tatcgcttgg
    14881 aaagtccgtt tgaaggcaat gatactgcat ggatgtttgt ctcaaaagac caaacagaag
    14941 ccatggtctt ttatttccga gtattagcag aagctgcgca tccattagtc acattgaaat
    15001 tagcaggatt agcagaagaa gcaacttatc aaatggaaga tgtaacattt gctggcgatg
    15061 aattgatgaa tctcggtttt tatattgact cagaactaca tggtgattat gcgacacaac
    15121 gcttttattt gaaacagata tagtagcttg aatttacaat ataagtgcaa caccaaaaga
    15181 gactcattcc aaacgaagaa aatatgtttt atttacacgc atttactaaa aagcagcctg
    15241 atttgaattg ggaaaatgaa gaagtacgtg aagaaatcta tgcaatgatt aactattggt
    15301 tagataaagg actaggcgga tttagaatcg atgcgatatt aaacatcaaa aaaaaactag
    15361 agtatggcat ctttgaatct gatggagaag atgggttagc cttcattggg ccctggattc
    15421 ttaatcagtc gggcattgaa gtgtggttaa aagaaatgag agagcggaca tttaaaccac
    15481 ataatagtat gacggtagca gaagccgatg ttcctaatga acgtttagat gaatatattg
    15541 gtgaagatgg ctattattcg atggtttttg attttagtta tacggatatt gatgtacctg
    15601 aaactggtga atggtttaaa gatagccaat ggacatggac ggatatgcgt accaatattt
    15661 ttacgaatca attagttacg caagataaag gttggggcgc cttatattta gaaaaccacg
    15721 atcaaccacg ttcaattaat aaatatattc cagaagaatg gatcaatgat tatagtaaaa
    15781 aaatgttggc gacgcttttt atgttactaa gaggtacccc ttttatttat caaggacaag
    15841 aactaggaat gaccaacata gaaatggcat cattagaaga ttttgatgat gtagctacgc
    15901 attctcagta taaacgggca ttagaatatg gattggcacc agagcaagca ttgaaagctg
    15961 tcaataaacg aagtcgggat aattctcgca cacctatgca atggaatacg caaaaaaatg
    16021 ccagattctc aacttctgat aatgtatggt taaaagtaaa tcctaattat ccagaactca
    16081 atgcagaagc tcagttaacc aatcaacagt cggtattcaa ttattatcat caattaatta
    16141 aattaagaaa aaatagtgta tacaaagacg tgctaatcta cggacaattt ctaccagtga
    16201 aagataaaaa tgaacatatt ttgctttatg aacgccaatt agaagataaa atcgtattag
    16261 ttatcttaaa cgttacagca acagaacaag aatattatgt agatgaacag taccaacaag
    16321 tattaatcaa taattatgaa gatgttttat tagatcaaag tcgaaaattg cgtttacgac
    16381 cgtttgaaag tattgtatta gctaattatg gaggacaatt atgaaaatta aaaacgaagc
    16441 aatgctcatc acctatgcag atagtttagg aaaaaacatg ggcgaattaa aacaagtctt
    16501 agacaaacat ttacaaggtg ttattggtgg tgtacatctg ttgccttttt ttccatcgac
    16561 aggtgatcga ggctttgcgc cgagtgatta tacgacagtt gatccagctc ttggaacatg
    16621 ggaagatgtg gaagcattag gtgaagatta ttatttgatg tttgatttta tgattaatca
    16681 catttctcgt gaatcaaaat ttttccaaga tttcaaaaaa aaccatgagc agtcaccata
    16741 caaagaaatg tttatccgta ttcatgaatt tttcccagaa aatcgaccaa cacaagaaga
    16801 tattgattta atctacaagc gtaaagacaa agcgccgttt caaacggtag aatttgcgga
    16861 tggtacgaca gaagaggttt ggaatacctt tggggaagaa caaattgatt tagatgtgac
    16921 caaagaagtt gtgaaagaat ttattcgaga aaccatcaaa gatatggcga accatggctg
    16981 ttctttaatt cgattagatg cgtttgctta tgcgattaaa aaattagata ccaatgattt
    17041 cttcgtggaa ccagatattt gggaactttt agacgaagtg cgtcaagaag cagcgaaata
    17101 tgacgtcgaa ttattaccag aaatccatga gcattattcc attcaaatga aaattgccaa
    17161 tcatgattat tatatctatg acttcgcctt gccaatggtc actctgtatt cactttatag
    17221 tggaaaatca gaacgtttag ccaattggtt gaaaatgagt ccgatgaagc agtttacaac
    17281 attggatacg catgatggaa ttggtgttgt ggacgctcgt gatttattaa cagatgaaga
    17341 attggattac acctctgagg aattgtataa agtcggggcg aatgtgaaga aggtctattc
    17401 aagtgccaat tacaataatt tagatattta tcaaattaac agcacctatt atagtgcatt
    17461 aggcgataat gaccgtagct atctgttagc acgtgcgatt caagtatttg cgccagggat
    17521 tccacaaatt tattacgttg ggttattagc aggaaaaaat gatattgaac tgttggaagc
    17581 aacgaaagaa gggcgtaata ttaatcgtca ttactatgat ttagccgaaa ttgaagaaga
    17641 agtgcaacgg ccagtgattc aacaattatt tgcactttta aaatttagaa atgaatctgc
    17701 ggcgtttgat ttggatggaa cgattgaagt agcaacgcca tcagaatcag aaatagtgat
    17761 tacacgtaaa aataaaaacg gtagtgatag tgcaacatta acggctaact tggatacgaa
    17821 agaatttatc attgagtcta aagatataaa aattttcact agtgttgcat taaaatttta
    17881 acctattcca tagaacaaaa aatcctttat actagcactt ggatgactta tccaagacca
    17941 ataaaaagga ctcatacatg gataagtata aaacaaatac agcatttaat aaatggtttt
    18001 ctgccataaa attaggaaaa ctttctgtca actcccaaaa atgtattttt gattacaatt
    18061 cgtatcgtaa aaaactgtct tttgaagctg tgctcaagct ctttctctac gcagttaatg
    18121 aagaaaaaga aagtttgcgt gatttgagta cagctcttat cgaccgatcg ctccaactag
    18181 aaaccgatgt gtcatcgatt agtcataccc aattgtctcg agcatttaat gcacttgaac
    18241 ctaaagtgtt agaagaaatt tttcaacaac tgttagaaaa agttcaacat caaatcaaac
    18301 caaccaagcg aaatcatctg tatctgattg attccagtac gttttcgctt agtttaaaac
    18361 gtcataagtg ggcgactttc cgtcaaacaa aatcaggtgt caagttacac ttaaactatt
    18421 gttatatgga cgatactacg atgtatccaa ctgattttac cttaacaaat gcccatgaac
    18481 atgatctcaa tcaactacca gtattggtga atcaaccaga ggcaacttac gtgtttgatc
    18541 gtgggtatta ggactttgaa aagatggatc agatgcattg ggatggctat ttcttcgtca
    18601 ctcggattaa aaaaaacacc aaagttcacg tgatagatac tctggaaaca tcgccagaaa
    18661 cagaaattct acgagacgag ctggttcgtt taggatctaa aacctatcta accgccaatt
    18721 tccgactagt cactgttcaa gataagaacg gacgtgtgtt tcaatttatc accaatcgaa
    18781 tggacgtctc ttcaaaagaa atctctgata tgtatcacgc tcgttggcaa atcgaattgt
    18841 ttttcaagca catcaaacaa cacatgacca ttaaaacctt cttttctcaa agtgaaaaag
    18901 gcgtgcagaa tcaattgatt ttaacgatga tttccgctct cctcacgttt ctcatcaaat
    18961 tggagacgaa aacggaaaaa tcagtgttcc aaatcaaacg attctttcgt tatttactgt
    19021 ttcagccctt tgaatgttgt ctagaaaaat taattcccac atgagaaaaa agaaatttgg
    19081 ctcgttactt ttggttttca gttgaataac agataaaaaa ttttctggaa aaagtcatcc
    19141 tctgcgtcag cacagttttt tttgacttat tttcagagga aatagttttc agaatattct
    19201 aaaatgtttt atgcaacact agtgatattt tttagaatta gatttctaat gtgcattttt
    19261 tgagtattaa ggagcaagta tacaaagatt tataaagctg attaaatttg aataaaaata
    19321 tgcaggaacg ctactatttt tagtagtgtt cttttttata tttgaataaa aaatgtggtg
    19381 gttaaatggt ggttataaca aaaaatgaat taatgaaatt aggttatggg aagtatcagt
    19441 ctgagagaat catacgacag gcaaaagcaa ttatggtttc aaaaagctat acctattacg
    19501 aaaatagaag attaggacta gtacctgttg aatctgtaga agaagtttta ggtatgaaga
    19561 tagaggtaga ggaggatttt cagaatcatg gttaatttta aaaaagataa aaatgggaaa
    19621 tatcatttta ctgctagttt gggattcgac aaacgaacag ggaaaagagt acaaaaaaac
    19681 gtagaggggt taaaacagtt aaagaagaaa aaaagcgtat accgaacttg tttctaatta
    19741 tggaaaagaa gcagaaactt ctcaaagtac tatgttgttt gaggagtttt tttataaatt
    19801 attcctacct tattatcaag ggaaagtaga agaaagaaca tataaaaatc ggttatctac
    19861 aatcaaaaaa acttttctta tttctttaaa atggagttaa gagaaatacg tccaattgac
    19921 attcaaaagt ggcaaaataa tttattgagc aaatataaaa attcttatgt aagaaacatg
    19981 tatgggattt ttcagatgtc attagatcga gcagttgttt tgggactact tgaaaaaaat
    20041 cctgcaaaag ttattggaaa tgtgaagaaa aggaaaacta cagttgattt ttggacaaaa
    20101 gaagaattta caaaagttat ttcaactttt tatattgatg atttgtacca acattttggt
    20161 tttatttgta tgtggttact ttttatgacc gttatgcgtt tgggagaagc cactgcatta
    20221 acttggaaag atattgattt tgaaaataaa aacttgcgtg tgaataaaac actctattat
    20281 aagagagcca aaacctatga gtttgtagaa cccgaaacgg cttctagtat tcgtacaatc
    20341 gaactagacg acaatacaat taccttttta aaagcttgga aggagcgtca agtttcaata
    20401 ggaggcatgt cgtttgtact ttcttataat ggaacaccta ctcagaaata tacaatccgt
    20461 catattatga gaagacatgc aacgatcgtt ggtgttcata taatccgcat acacggatta
    20521 aggcatagtc atgcttcttt gcttatatca caataaaaag atgcacttcg aattaaagaa
    20581 aggttaaaac atgctgatgt acaaacgact ttaggaactt atggtcattt gtatctaaat
    20641 tcattgagag agataacaaa tgatcccaca gattatatcc aaactaattt ttctaaaatc
    20701 aaaccgacac ctatttacga taataaaaaa taaatgataa acaaaagtag gatcacacca
    20761 aaagaaacac actagaaaaa ctataataca ggcttttcta gcgtgttgtt tcttatttcc
    20821 gcctatttat acattatact cataaagcat ttaatgcatc gatcgtagcc gcattgtcta
    20881 tatgggtata taaagttgtt gtattggtac ttgaatgtcc caattgttgg gcagttaata
    20941 ccaatgaatt tgtctgttgg tagagtctag tagctaatgt atgacgcagt ttgtgagggc
    21001 tgacttgaat tctaaagcct tttgaatatt ttgctaccat ccgttcgatt gcaataccgg
    21061 atatacgctt ggcttctcct tggtaaattg ctagaaataa agctttttcg ttttctgtta
    21121 ctttatatcg ttctttccga atagctagat aattagctaa atattccatt gcgaatgctg
    21181 ctatattgac agcatctctt ttatttcctt ttctagttac taccacatta ttcttatcta
    21241 aagataggtc atctaaatta atatttataa cctcggagag tctcaatcca gatcctaaaa
    21301 tcagtgcatt gatcgccagg tctctttcta catctcgttg ataatagatc aaagctcgtt
    21361 tactcaaagt atttttatat tcattggcaa tataatctag ataattttgt gtatccttat
    21421 ctagaaataa ttttttctca atggcattgg cacgtgcagc taacgtagtt ttatctgtga
    21481 tggttgctac ttgatccata ggattattgt ctaagtatgg ttttcctaga ggattattgg
    21541 aacttttcga aagatattta aatagaacct ttaatgccgt gatatttcta ttgatagtag
    21601 aagtagatag acctaagtta ttattatctt tgagcttagt ttttgttgtc tcttttttta
    21661 cattttcttt tgtgcgacgt ttcaaatgag atttgtacaa gtttaggtca taggcagtta
    21721 actgtcttat cgaggtgata gaagtatcag gaacgataga ctcactcaga agccattcat
    21781 aaaagagctt ataatttttt agatattcta aagtggttcg aggtgattga tctttatcct
    21841 cacgatcttg aacaaaatct tttatataat cgggcatatg aagtatgatt gtatctattt
    21901 tttgatatat ttctgtattt ttcatgtcat tcatcctttc aataagtagg ggagaagagt
    21961 atctctataa ttggactata ccaattatag aatgttttgt caattggtat gctctaaaat
    22021 ttattaattt gtttcaaaaa aagcatataa acaggtatac gtttaataat ttaaaataaa
    22081 tggtatacat aaatagggaa ggaaatagaa gggagcaatc taaatttttt gaattttctt
    22141 aattaattat atagaaagaa caatctttct atagagttag acatgagagg gactatgctt
    22201 gatttaaaat caacaagcgt atatactgta tatacaaaga aaaaggaggg agaagactat
    22261 ggcaattaaa gaaagaaaat ttagggaagt ggggaactca gtagtattaa ctatttcaaa
    22321 ggagtttctt aaagaaaatg ggttaaatcc cggagatacg gtttttttag atgaagaaaa
    22381 acttcgagcg gcaattacaa aaaaacaagc cttatctgat gaaattgatt tagcaatatc
    22441 tcaagcatta actgaatatg atactacttt tagagagctt gtcgataaat gagtattcat
    22501 tacctagaag ccaaagatat tataaaaatg aatgtaatcc aaataaaaaa atactctcct
    22561 aatgaacccc ttggtgtaaa ggatgcgaat gctttagaaa tgtgtgtagg gcaacttcga
    22621 gcctcagctt ttggtgaaga ggtttatcct tcgatttatg aaaaagcagc tatcctactt
    22681 atacaattga taaagaaaca tccttttcac aatgcaaata aacgtactgc tttcttagct
    22741 acttttgtct ttttgaaaat caatggacag cttttagcta ttgatcaaaa agatgctgta
    22801 aatttagcgg tatatattgc tacgtatgat aaggatttcg atcaactaaa atatgaagtg
    22861 atcaacacta taaagacaca tacgcagccc atttaaggag ttactgtatg tgtctttata
    22921 gggttattag ctgctatagc gaaataattg aagtaaatta caaatagcta taattcaaat
    22981 ccaactatat agaaaacgta aattttctat atagttggat taagataacg actatttatt
    23041 ttatcatatt tttactatac cctatacttt tacttggtat atttatgaat aatcgca
//
LOCUS       DAHUVV010000034        22994 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.034, whole genome shotgun sequence.
ACCESSION   DAHUVV010000034 DAHUVV010000000
VERSION     DAHUVV010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.034"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(111..566)
                     /locus_tag="NK767_001351"
     CDS             complement(111..566)
                     /locus_tag="NK767_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694353.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="NK767_001352"
     CDS             complement(720..1928)
                     /locus_tag="NK767_001352"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCH6694354.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="NK767_001353"
     CDS             complement(2040..2372)
                     /locus_tag="NK767_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCH6694355.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="NK767_001354"
     CDS             2520..3395
                     /locus_tag="NK767_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCH6694356.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="NK767_001355"
     CDS             complement(3477..4058)
                     /locus_tag="NK767_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCH6694357.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="NK767_001356"
     CDS             complement(4064..5323)
                     /locus_tag="NK767_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCH6694358.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="NK767_001357"
     CDS             complement(5325..6371)
                     /locus_tag="NK767_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694359.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="NK767_001358"
     CDS             complement(6606..6881)
                     /locus_tag="NK767_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCH6694360.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /gene="der"
                     /locus_tag="NK767_001359"
     CDS             complement(7124..8434)
                     /gene="der"
                     /locus_tag="NK767_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCH6694361.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="NK767_001360"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="NK767_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCH6694362.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9980..10660)
                     /locus_tag="NK767_001361"
     CDS             complement(9980..10660)
                     /locus_tag="NK767_001361"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCH6694363.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10709..11344)
                     /locus_tag="NK767_001362"
     CDS             complement(10709..11344)
                     /locus_tag="NK767_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCH6694364.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="NK767_001363"
     CDS             complement(11410..12813)
                     /locus_tag="NK767_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCH6694365.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="NK767_001364"
     CDS             complement(12800..13831)
                     /locus_tag="NK767_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6694366.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWTLFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="NK767_001365"
     CDS             13862..14083
                     /locus_tag="NK767_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCH6694367.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="NK767_001366"
     CDS             complement(14248..14985)
                     /locus_tag="NK767_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HCH6694368.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="NK767_001367"
     CDS             complement(15138..16478)
                     /locus_tag="NK767_001367"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HCH6694369.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..18238)
                     /locus_tag="NK767_001368"
     CDS             complement(16715..18238)
                     /locus_tag="NK767_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCH6694370.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18616..19227)
                     /locus_tag="NK767_001369"
     CDS             complement(18616..19227)
                     /locus_tag="NK767_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCH6694371.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19350..19475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19557..20273)
                     /locus_tag="NK767_001370"
     CDS             complement(19557..20273)
                     /locus_tag="NK767_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCH6694372.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="NK767_001371"
     CDS             complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="NK767_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCH6694373.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20832..21629)
                     /locus_tag="NK767_001372"
     CDS             complement(20832..21629)
                     /locus_tag="NK767_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCH6694374.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21599..21970)
                     /locus_tag="NK767_001373"
     CDS             complement(21599..21970)
                     /locus_tag="NK767_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCH6694375.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="NK767_001374"
     CDS             complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="NK767_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCH6694376.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6447 a         4912 c         3900 g         7735 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc cgttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggtcca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aattaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa
    18181 taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct
    18241 tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa
    18301 aagagtatca gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg
    18361 tttttcccta ttccctcctc ttttttcagg tttttaaagc tttcttcttt ttcattgtag
    18421 cgtttccttt tttttataaa caaacatttt cgtcttttaa aaggagaaga atttcacatt
    18481 tgcaaagaat tgattgattt ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa
    18541 aacgtttaca ttattgcatg tcttgtattt ttttccaaaa aaaaagacca agaaatttct
    18601 tgatcttttt gatatttatt tgactgctgt atgacgttgt tcgaactgat gttgttttcg
    18661 acttaaccat ggaagaagtt gcgcatgcag cactaaaaag acagcactca cgatgaatcc
    18721 tttgatcaag ttgaaaggaa cgatcccaat cagtacatag tttgctaaag acatgcccag
    18781 catctgtgtt gcgttcagcc ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt
    18841 tgcgatactc ataaaaatag ttagagcgat ggttcctgta atgacaccta aaaatttgtt
    18901 tcttccttta tgctgtttct ttacactaaa gaaataataa atcgggaatg taaagatcgt
    18961 tgttgataag aaactagcag tgtctcctac caaattatcc ggagaaaaac ctgtagtcag
    19021 cagatgtaat actgaacgca agaacgctgt tgcaactccg gccgctggac cgaataagaa
    19081 catgctgatc aataccgaga tatcactaaa atcgatcttc aaaaaactaa aagccggcat
    19141 gatcggaaaa gcaaaatact gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac
    19201 cattttctgt acacgggtgt tcttcatttg aaatcctcct gttaggactc ctcttcagcg
    19261 aactttttga cgtttctctc caaatgaaaa aactctattt tccagggaaa atagagttag
    19321 agttatttga tcaagcgtcg tcaaataagc tctatcttct ttatccagac tatactgtcg
    19381 gtatcggaat ttcaccgatt cagctatttt tttgcaaaat aggtcgtgga ctataaccac
    19441 cggtcgggaa tttcaccctg cccctgaaga cgaaccttat ataatttttg tttacatatt
    19501 cattatacat atctattcaa aaaacgcaac atctaacact tataaaaagt aagtgttcat
    19561 tttgagtggt tcagcaaact gctgacttct tttttgttca gttcacggta atccccagga
    19621 cgtaggcctt gtaaagtcag atctccgtat ttttcacgtt ttagtttttg cacaggaaga
    19681 ccaactgctt gaagcatatt tttcacttga tgattacgtc cttcgtggat cgtcaattcc
    19741 acgatactgt ggtttgtctt aggatccact gataaaattt ggaatttagc tggagatgtt
    19801 tttcgtcctt cgatctttat tccttttgtc aaaggtgcaa gcatcgattt tgtagcgatc
    19861 ccttttacct ttgcaacgta aactttatcc acttcatgtt ttggatgtgt caggcgctga
    19921 gcaaaatctc catcatttgt cagtagcaag atccccgaag tatcgtaatc cagtcgacct
    19981 acaggataga tacgttcttt taccatcggc aaaagatccg taacaacttt tcttcctttg
    20041 tcatctgcaa cagcagaaat aactccttta ggtttgtaca ataaatagta accgtattct
    20101 tcttgataaa ttgggacttc atccacttcc acagtgtcat gtttcccaac ttttatccct
    20161 aattccctta caacttgtcc gtttactttt acacgtcctg caaggatgaa ttcttctgct
    20221 tttcgacgag aagtgatgcc agcgtgggca attactttct gcaatctctc cattacaatt
    20281 attctccttt attcattcca tcaaagaata gatctggtga cacgtcttct tcaagtgaaa
    20341 tttccatttc ttgtgtatca ggcagttctt ctaatgattt caaaccaaaa taatctaaga
    20401 agtagccagt tgttccatat aatatcggcc gacctggtcc ttctactcta cctttttctt
    20461 caatcagttt atgggcagct agacgctgta ccgctcctga aacctgaact cctcgaatct
    20521 gctcgatttc agctcttgtg attggctgtt tataagcgat gattgctaag gtttcgacgg
    20581 cggcttgaga taatgtgttt gatgtccctt gcgcatatcg cttaagtaag ggagccagct
    20641 cttttttagt cgttaataca taatggcttt ccgtttcaaa aatatgcaaa gctgagtgcg
    20701 agttttgagt gtaagattct tttaattctt tcactaaact tgtgacttct gcttcgctga
    20761 gatttaacag tccgcttaat tcttcattac tgatcccttc ctcgccaaca ataaaaagaa
    20821 gggcttcaag ttcacttaat ttcgtcatgt tcggtatctc ctactgctgg ataaagcata
    20881 attgattcat agttttcttt ttgttgcgca atgatctgcc cttttttcat caattctagc
    20941 aaagccataa aagtagtgac aacttcactt ttagaaggtg actcaaaaaa agaatcgaaa
    21001 gtacagccat gcgcatttct ttttatacgc tcttcgataa ataccattcg ttcttcgatc
    21061 gttgtgtcat cgggcgtgat cgttttttcc actttttgag aaaatttctt acgttctaag
    21121 attttctgca tggcacggat caaatcgatt tttttgactt gtcctcgctt caacggcttt
    21181 tcagcatccg ctaacccatc gatgttcata ggttcttttg tgtaataaag acttcgttcc
    21241 tctgcttttt ctgtcagtct ttcagctgca tatttatatt tacggtattc taaaagctga
    21301 gtgaccaatt cttctcgagg atcttcttcc cataattctt catcgtctgt gccgaattct
    21361 tggacaggaa gcaatgtctt gcttttgatt gccatcaatg tagcagccat cactaagtat
    21421 tctcctgcaa cagctagctc gagtgttttc attgtatgga tataattcat atattgctcc
    21481 gtgatcgtag cgatcggtat atcatatata tcgatttcca tttgctgaat cagatgaagc
    21541 agcagatcga gaggaccctc gaatacatct aatttaatat tgatctccgt caactcattc
    21601 actctttctt ctttttcttt gctagccatt ttccaatgta aggctctgtg atttcagttg
    21661 cacggatgga gacatccggg tacttttcct gtaattcatc taacatttga aagcccatat
    21721 tctcaccgcg ataagacgga ttcaaagaaa tgtcatggat gatcagctga tcgttcgttg
    21781 cccagtaaat accaataatc ccttgaatgt tggttgaacc ttctggcgta aagaaaaaaa
    21841 gttcataatc gtcttcagat tcgtacgtat caatctcctt caacaaagaa gttttacctg
    21901 tcagtttctt atggaaactc aatagtccca tagcaatttt acggttttct ttccggtaat
    21961 gtgttaacat cttctcacct tcctatgact aagcccttgg aaaatgctgc ttataaacat
    22021 ccgtcattct tttcttcgtg atatgtgtat aaatctgagt cgtagagata tccgcgtgac
    22081 ctaacaattc ctgtactgtc cgcaaatcag ctccattttc tagcagatgt gtggcaaagc
    22141 tatgccgcag cgtatgtggt gttacttctt tgtaaatccc agcctctcgt actaactgct
    22201 tcagattttt ccagattcct tgccgagaaa gacctttccc atgattattg acaaatacat
    22261 gcatttcaga tgcgtccttt acaagtaatg gacgtgcttc gtctaagtag cgttccagcc
    22321 attggatcgc ataatcacct aaaggaatga tccgctcttt atctcctttt ccaactgtct
    22381 gtaccagtcc aagagaaaga tgaagatctc ccaatttcaa tccaatcaat tcactgacgc
    22441 gcatcccagt agcatacatg acttccaaga ttgcacgatc tcttatccct aacgtttttg
    22501 tcgtatcagg tgcttcaatc agcttttcta cttctgtcag agtcaatgta cttggtaatt
    22561 tctgtgcttt cttcggcgta tcgatatgtt gcatcggatc atgatcagtg atacgctctt
    22621 gccgtaaaaa ctgatggaat cttctgagac ttgagatcat ccgagtaaca gttgctgtcg
    22681 cttttttttc ttcatgcaac ttctccagaa aacggatgac taaaaagcga tcgacatcct
    22741 gccatgaagc aattttctgc tcctgtagaa aatgcaagta ctgttccaaa tcacgttcat
    22801 agctttgtcg tgtattggcg gacaatcccc gttcaatatt caagtagtgg agataatcgt
    22861 tgatttgctc tttcataata atccccctca tgcctctcta tcatatcaga taccgcttca
    22921 ttctgatagt ctttttccat aatctgagca aagaaaaagc cgtctagaca ctcagacgga
    22981 ctggtttctt tttt
//
LOCUS       DAHUVV010000035        22895 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.035, whole genome shotgun sequence.
ACCESSION   DAHUVV010000035 DAHUVV010000000
VERSION     DAHUVV010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.035"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            68..286
                     /locus_tag="NK767_001375"
     CDS             68..286
                     /locus_tag="NK767_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694377.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            283..696
                     /locus_tag="NK767_001376"
     CDS             283..696
                     /locus_tag="NK767_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694378.1"
                     /translation="MIPKFRAWVKPDILSNHLNGVIAEALPNFIYGNCLVLREDLEED
                     KQFEEIIDFLDIELMQSTGMKDKNGVDIFEGDIVKTLDADLKVTFSTIKFEEGAFCVD
                     YKDLGTEFDFLYFVDSPLEVIGNIYENSELLEEQR"
     gene            693..1277
                     /locus_tag="NK767_001377"
     CDS             693..1277
                     /locus_tag="NK767_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCH6694379.1"
                     /translation="MNKQELIDKLSSLVGSIEDFKGISTFHAGKYAGLEHALELIQQL
                     NESQKPVIPQLVAGWIEKSTDNLTKAEKIAYLIKSKDGDSYYFCDWFVRDGILTQEQG
                     EELLAWATRQSYETLLSLYNGYEVEKEPLYEVIIGDLYLIKKFNNRNDFCFDISRSLC
                     TWEKSAYQLTEAEIKEIDERYWPFAVPVEEVVER"
     gene            1274..1573
                     /locus_tag="NK767_001378"
     CDS             1274..1573
                     /locus_tag="NK767_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694380.1"
                     /translation="MSDYLTAEQYEYARKTGDWESGKVLTHGQALNDNQKIVLEWLEE
                     YYAKSQWPFPFYVLKHAANDMPTNIFGFYDDFTMKEEFEVLAAFAQWGLKQEEAE"
     gene            1570..1767
                     /locus_tag="NK767_001379"
     CDS             1570..1767
                     /locus_tag="NK767_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694381.1"
                     /translation="MNKRQKNKQFKKQLIKNGYPDKKVKLYCSECGNRLSFKNEYHKR
                     HLLCNEFCFMHSVGLGWNDFL"
     gene            1783..2088
                     /locus_tag="NK767_001380"
     CDS             1783..2088
                     /locus_tag="NK767_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694382.1"
                     /translation="MNVQVYLKRDLARFPRSWDEYSCPFEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            2112..2408
                     /locus_tag="NK767_001381"
     CDS             2112..2408
                     /locus_tag="NK767_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694383.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDIKLINDK"
     gene            2484..2900
                     /locus_tag="NK767_001382"
     CDS             2484..2900
                     /locus_tag="NK767_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCH6694384.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHVNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            3161..3574
                     /locus_tag="NK767_001383"
     CDS             3161..3574
                     /locus_tag="NK767_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694385.1"
                     /translation="MDKEIKATVKLDLTELKELLNKASDQVEQIKYQGMDRKRLIDII
                     EQQRLELIQKEAVCKEYKKHLEQVIEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTP
                     VFNSVKSEKLESISTALVEKAAEMNSLANEVSRLI"
     gene            complement(3567..3758)
                     /locus_tag="NK767_001384"
     CDS             complement(3567..3758)
                     /locus_tag="NK767_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCH6694386.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDMTK"
     gene            4229..4441
                     /locus_tag="NK767_001385"
     CDS             4229..4441
                     /locus_tag="NK767_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694387.1"
                     /translation="MNENQLRELFKTNEANQTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNEGQAMEFMMRTFSANNQQRE"
     gene            4448..4672
                     /locus_tag="NK767_001386"
     CDS             4448..4672
                     /locus_tag="NK767_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694388.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            4699..5232
                     /locus_tag="NK767_001387"
     CDS             4699..5232
                     /locus_tag="NK767_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCH6694389.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            5219..6463
                     /locus_tag="NK767_001388"
     CDS             5219..6463
                     /locus_tag="NK767_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCH6694390.1"
                     /translation="MGLVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDIAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRVKRMF"
     gene            6476..7972
                     /locus_tag="NK767_001389"
     CDS             6476..7972
                     /locus_tag="NK767_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCH6694391.1"
                     /translation="MDKVNEFEYGTDIHYSNDVNTNYVKFSVESNLHYRFSSAEDLLN
                     DSNTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKDRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNAETSADRGVFDSDKETDTEVEEDEST"
     gene            7959..8222
                     /locus_tag="NK767_001390"
     CDS             7959..8222
                     /locus_tag="NK767_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694392.1"
                     /translation="MKARKKPVTVEIVQLKMLSARSYRKCKEFVGEAWIDHNNMPNGL
                     PGIETLEGTMEISDGDYIIKGVHGEFYPCKPDIFIETYEIIEE"
     gene            8227..9183
                     /locus_tag="NK767_001391"
     CDS             8227..9183
                     /locus_tag="NK767_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCH6694393.1"
                     /translation="MKSQDYFIKREKAWQEQQIKDDKKRMNEIKKRLQYAQDAIQKEI
                     DAQWNSFSNGQKITRSEAMKRANEMDVKAFARKAKKYVKEKDFSPTANKELKLYNLTM
                     RVNRLELLKANIGLELIATFNELDKYFSNELTKAGLDELKRQAGILEMAIAKSGYAKL
                     VEQVINSSFRADGFATFSERLWMYQAELKADLDKLLVRSVTMGRNPKQLAPELSKFLT
                     EEGRENTKFNTQRLMVTETTRVQSGIQRESYLDANIEKYGWVTEPNACRLCKSIAADG
                     PYLVSEMEVGKNMMPVHPFDRCSTYPIVDRDAFEKSLKERRL"
     gene            9261..9488
                     /locus_tag="NK767_001392"
     CDS             9261..9488
                     /locus_tag="NK767_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694394.1"
                     /translation="MHLYQNDAQFKEDYDAVKQHLIDVLEPIVKEVYETARKVIEPVV
                     QSITELVQSNPEYFKALQNNMNQSVFQSASM"
     gene            9503..9676
                     /locus_tag="NK767_001393"
     CDS             9503..9676
                     /locus_tag="NK767_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694395.1"
                     /translation="MENEEAIVRAIASLNKILQNEKVLRVSVETDSEWEDGIQSIDIS
                     VKYLPSGDVSEAE"
     gene            9791..10393
                     /locus_tag="NK767_001394"
     CDS             9791..10393
                     /locus_tag="NK767_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HCH6694396.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPTEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELEAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            10408..11295
                     /locus_tag="NK767_001395"
     CDS             10408..11295
                     /locus_tag="NK767_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCH6694397.1"
                     /translation="MAQTWNPDNVTVYETKEGKIPDKYNTLIINDIMENSKVMQLAKY
                     EEMTDKEKKFEYFAEGPGAYWVGEGEKIQTSKPKWLQATMVAKKLGVIIPVSREYLHY
                     KMSDFFTVMQPKIAEAFYKKFDAAALLNTDNPFPQSLEESVVAAANVIKGPLTYDNIL
                     ALEDALGKNEFEPNAFISNRKNRTELRSAAQTVGTNVEFIYDRSANTIDGLPVVDLKA
                     LGKGELYAGDFNYMFYGIPFNISFKISEDAQLSTLKNEDGTPVNLFEQELVALRATMD
                     VGFMIVKDAAFGKIEPAGE"
     gene            complement(11252..11539)
                     /locus_tag="NK767_001396"
     CDS             complement(11252..11539)
                     /locus_tag="NK767_001396"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694398.1"
                     /translation="MCDPSSVFIVVVYLPAGTVSVQVILPLEETVNPLVTGAIEYVTV
                     LFVAFCGATVAVNCLLPAVPATALEVLGVTVTPITGIVTPPPVQFYRTQHL"
     gene            11589..11918
                     /locus_tag="NK767_001397"
     CDS             11589..11918
                     /locus_tag="NK767_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCH6694399.1"
                     /translation="MVIKDDVKKLLSGSTDDKLEVIERRTRERLASLLGVSVTPESLE
                     YIVFDVTNKRFNRIGQEGMTSYAQEGLSMAFPDSDFSEYASEIDDFKRKDDEELYKPR
                     KGSVRFL"
     gene            11915..12229
                     /locus_tag="NK767_001398"
     CDS             11915..12229
                     /locus_tag="NK767_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694400.1"
                     /translation="MRFLDEVIFEKDGEGNRYDPELGEWIEEAPITVTTNANVTDLGT
                     DRSVAIFGDIRQGAKVIRTMPLFVVPKYDRIIFNGKTYKDVAQRSLLSTNSIIVQEVI
                     AK"
     gene            12226..12597
                     /locus_tag="NK767_001399"
     CDS             12226..12597
                     /locus_tag="NK767_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCH6694401.1"
                     /translation="MKVSLGYKGVDELLKHLEEAVTLRDVQMVVKTNGAELTKRMQEK
                     ARFNGHWEGDVFVHPTGFTRRSIRMWLLEGGFVAQVGPQSDYSPYLEYGTRFMSAQPF
                     VGPAFNVQKAIFMKDMQRLFK"
     gene            12594..12983
                     /locus_tag="NK767_001400"
     CDS             12594..12983
                     /locus_tag="NK767_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HCH6694402.1"
                     /translation="MIKTRDQSLFDEMFARSLALGYQTYDYKPLDDVGYPFVELESSQ
                     TVHNPNKTDIKGTVNLTISVWGLQKKRKQVSDMASALFNQALQIQATEGYYWDLNYQA
                     SGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            12994..13506
                     /locus_tag="NK767_001401"
     CDS             12994..13506
                     /locus_tag="NK767_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HCH6694403.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDD
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            13552..13920
                     /locus_tag="NK767_001402"
     CDS             13552..13920
                     /locus_tag="NK767_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HCH6694404.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFIDGNMEFGMGLSAKVL
                     PELRSYNANTLSRVLEIANRTEEETITLDEMDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQAE"
     gene            13974..14261
                     /locus_tag="NK767_001403"
     CDS             13974..14261
                     /locus_tag="NK767_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694405.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            14278..17100
                     /locus_tag="NK767_001404"
     CDS             14278..17100
                     /locus_tag="NK767_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCH6694406.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEEKSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEEQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQRLVVA
                     IGAIAIAIGPVLYALGMLVKAFQTMKVGLGVLGNGISLFKKLGSAIGFLTSPVGLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTIQEAPGKAADWIKNKWTETKEFFSSIWDGIKEAASSAWEGIVNILTP
                     YVIAIKNVFQPMIDFFTNLWSQIGSIAGSAWEIIKTAVMGPILLLIDLITGNFNQLKE
                     DASMLWTTLTTNIQNIITTFVDIVVGYYTALKDTVINIWNVLTSTIKDVWNSFTTWIK
                     ETTNNIVNSIKQGWNNLKQGTIDLFNNMIQGAKDLWNSFKAWFINLVIGTKDNIIQGW
                     ENLKQGTIDTFNNLVNGAQEVWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDS
                     FLEGLQNAWKSVQDFVGGIGDWIREHKGPIRYDRKLLIPAGQAIMNGLNAGLTNGFAS
                     VQSNVGNMANMIADSFTRTPDIDLSANLKNANRNFTTQIEHSVNYGKNKRPAVFNIRL
                     GNQVFEAFVEDISNIQGKEADINLLF"
     gene            17118..17900
                     /locus_tag="NK767_001405"
     CDS             17118..17900
                     /locus_tag="NK767_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCH6694407.1"
                     /translation="MEWHNPMYEFRDTVKNDSQKTWIPTSALNYDGKFIENYIEGYQT
                     LYVEGREMVSLEIESEAVSIGVRISSQRLPERILTIHFKLEEKNPIEFQRSFNKLMRL
                     LYRDKDVEIHFNDELDMYYYGRYQTCDNIPGNVHSVISSFSIICSDPRKYTRIFETNG
                     IVSEYLPYEVAPISISLKANNDGSLRITNGRQNISVTNSMIKKGDFIEMDIAEGKVFV
                     NGVNKTRILDLTSSFKNFMVRTGDLVKCDNGTPLIRYRGVWL"
     gene            17900..20674
                     /locus_tag="NK767_001406"
     CDS             17900..20674
                     /locus_tag="NK767_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCH6694408.1"
                     /translation="MDKDVYFFDDSQKLIKIVGEDKLFSVVQEKEITPSKDELINDKL
                     AVSMEFDDEIKESAYMAVRESESSFSMYKIIGIADPGSLLIFTGINFGPDELDAYIIN
                     DIRPANEFFQKTIQRVIDFTLGEWRVGHLDSTLPAVSMTFYYCSIREALKNLQTLGCE
                     IVFRCNLSGEGITDKWIEVYKQIGEYSNERYEYGDKALTIEKEVDRSNIYTSLIGRGR
                     GEEVGDGYGRRIEFDQVYWSKSKGDPLNKPTGQIYLEIPEMTEKYGIPTKNGKRRKRE
                     KVIIFEDCEDPVELIQLTYQELVNCSRPLVQFKATIFGADSLGNIIRIHRDDRGYHYE
                     TRIFSVKIDRLTGKVETGLGDNLNTSSTRQASNTQTAIQTLDEKKMTFYESTEVSKWQ
                     SDIIRGAKGGSIIMMNPWDTGKGESRQPYQMVWMNGDSIDTSNHFLVANSEGIGFIDG
                     KFNESNFKTAWTIDGNFNANYIQSGRIRADIFETSFNAVGDQLKLVKGALQIVNSNKK
                     IMELTKKGMEFWNTKESIGTIGTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILI
                     SPKVGKGLVLLGNGKAIYFGDLDVQGKLTVNGKEITGNNSGGSDPGTIPPQLTTEAEK
                     RAWKIWTMLKARGYSEYAAAGILGNIQGEVGASMNPDTEQLGGPAYGIVQWDGSAYPL
                     VGSPTWNGREYVQRLMNTAGIQEDYRSIEAQVKLLDWCMFNGQWLGKVNPTTVSGFKS
                     INDAKSAAYAFEMNFERPASAHPERQNYAQSWYNKLHGLTSPEPGGNFICPIQKPVTV
                     TSECGWRTSPINGGQEFHNGIDLVNGNPNTPVFAALDGEVVQAGANYYDWYGNYVVIK
                     HNNGKWTGYAHLSRIDVSVGQKVQKGAQIGLMGTTGPSTGEHLHFQIMKNYWPQPVVD
                     FENPRNYIQF"
     gene            20687..21595
                     /locus_tag="NK767_001407"
     CDS             20687..21595
                     /locus_tag="NK767_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HCH6694409.1"
                     /translation="MSKWNVVLSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            21595..22215
                     /locus_tag="NK767_001408"
     CDS             21595..22215
                     /locus_tag="NK767_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694410.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            22219..22668
                     /locus_tag="NK767_001409"
     CDS             22219..22668
                     /locus_tag="NK767_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694411.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            22668..>22895
                     /locus_tag="NK767_001410"
     CDS             22668..>22895
                     /locus_tag="NK767_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694412.1"
                     /translation="MADIVQLKEDGVAKYLKTHVKAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTG"
BASE COUNT         7993 a         3546 c         5087 g         6269 t
ORIGIN      
        1 tataaagaaa tattagaaga gtatttgagg aggacagcaa atgataaaaa aactcgttca
       61 attcagcatg gatttatatg atatcgaatc aggagcaaca ctatctgtgg aatcggacca
      121 tctaatcata aattttggtg gaaagcgcca gattattttg tgggtagttg atgatgtact
      181 gtttccagaa attgttcatg atttcgaaga atcaaaagcg gttgagtttg aaatagtgaa
      241 aaaagtaatg gaattgattg aaaaatacga ggaggacagc gaatgatacc gaagtttaga
      301 gcgtgggtaa aacctgatat tttaagcaat catcttaatg gggtgattgc tgaggcatta
      361 cctaatttta tttatggcaa ttgtttagtt ttgagggaag atttagaaga agataagcag
      421 tttgaagaaa ttattgattt tcttgatatt gaactcatgc aatccacagg aatgaaagat
      481 aagaatggtg tggatatatt tgaaggggat attgtcaaaa ctctcgatgc tgatttgaaa
      541 gtaacttttt caacgataaa gtttgaagaa ggcgcatttt gtgttgatta caaagatctc
      601 ggcacggaat ttgatttttt gtactttgta gattcacctt tggaagtcat cgggaatata
      661 tacgagaata gcgagttatt ggaggaacag cgatgaataa acaggaattg attgataaac
      721 tatcaagcct tgtaggtagc atagaggact ttaaaggtat aagtactttt catgctggaa
      781 aatatgcggg tttagaacac gcattagaac taatccaaca actaaatgaa tcgcagaaac
      841 cagttattcc acaactcgtg gcaggttgga tcgaaaaaag taccgacaat ttaacaaaag
      901 ctgaaaaaat agcgtattta atcaaatcca aagatggtga ttcatattat ttctgtgatt
      961 ggtttgtgcg agatggcata ttgacgcaag aacaaggaga agaattactc gcttgggcaa
     1021 cgagacaatc atatgaaaca ctattgagcc tatacaacgg ctacgaggtt gagaaagaac
     1081 cgttgtatga agttattatt ggtgacttat atcttatcaa gaaatttaat aacagaaatg
     1141 atttctgttt tgatattagt cgctcgctat gtacttggga aaaatctgct tatcagctaa
     1201 cagaagcgga aatcaaagaa attgatgaaa gatactggcc atttgctgtg ccagtggaag
     1261 aggtggtaga aagatgagcg attacttaac agcagaacaa tatgaatatg ctagaaaaac
     1321 aggagattgg gaatcgggta aagttctcac tcatggacaa gcgctcaacg acaaccagaa
     1381 aatcgtgttg gagtggttag aagaatatta cgcaaaatca caatggccgt ttccttttta
     1441 tgtgttaaag catgcagcaa acgatatgcc gacgaatatt tttggttttt atgatgattt
     1501 tactatgaaa gaagaatttg aagttctagc agcattcgct caatggggat taaaacagga
     1561 ggaagcggaa tgaataaacg ccaaaaaaat aaacaattta aaaagcagtt aatcaaaaac
     1621 ggttatccag ataagaaagt gaaactttat tgttcagaat gtggaaacag attgagtttt
     1681 aagaatgaat accataaaag acatctattg tgtaacgaat tttgctttat gcatagcgta
     1741 ggtcttgggt ggaatgattt tctatgatga ggaggaagcg gaatgaatgt tcaagtttat
     1801 ttaaaaagag atttggccag gtttcctcga agctgggatg aatattcctg cccattcgag
     1861 gcagaagaag agagctatct aatgtctgct aacatgatag aaattacaag agatgatgcg
     1921 gatgagtttg ataaaaaaag atcatggtac tcggtttcga atccaatgta tacggctgta
     1981 atagaagatt atcatatatc cgacagattt attctggttg atctagaaag acctaagaaa
     2041 cgaattaaac agtatagacg ttgcgggttt tcaacaaaga aatggtgaaa attaaaacag
     2101 gaggaagcgg aatgaaacta aaagacggat tttactccag cagccacggc atcggcggtt
     2161 taatgctaga tatgccgaca aagaacccta aaacacgtaa gaaaccaaaa ttcaaagtcg
     2221 gcgacatggt tcgatgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac
     2281 atgtttataa tcactcagcg atcattcgta ttgaaaacac gatggaatgc gacaagtggt
     2341 tagcgaaaag caaagagaat ttagctgtag cgagattggt ggatattaaa ctaatcaatg
     2401 acaaataaaa aagccggatc gctccgactg atctaataaa ttccacaagt ttattatatc
     2461 acataaagga gcggtttgac ttgatgcaat tgttacgaga ggtagatttc aaacagacaa
     2521 gatgtaatgc gagagatgtg ctgaagaact ttcggcgctt ggagcggatg gcaggtcgct
     2581 ctttgataga cattaagtcg cctatcataa ccgatatgcc gaaggcacca aagcacgtca
     2641 ataaggcaga agacgcgatc attcaaatga tggatataga agcagagaga gacgcgattt
     2701 tagcggcttt gatggctctc agtctgatta gtcgtcagat actctactac agcttctgtg
     2761 acgtaaataa gcactctaat tatgaaatag gtcaattgat acgaggatac ggagagaaga
     2821 atgtagagaa gctgaaatcc atcgcattga tcgaatttgc tgaagcatac aaaaaaggcg
     2881 tattagttca gtatcgttga ttttgtaggg tttttgtagg gatagtgtag ggtttttgag
     2941 tgttttaacg tgatattatg gtagtgtcga aagattagtg ataggtctga gacaaaataa
     3001 taataaaagg aacatcgttt tattattgtt tcacaattaa gcttcgatag acagcaacgg
     3061 aaatattaag aataaggatg tgaatttcaa ctccttctga ttgttcttat tatctatcat
     3121 ccgttgctgt ctattaattt atgtattgga gggaaaacga atggataaag aaatcaaagc
     3181 aactgtcaaa ttagatttga ctgaactaaa agaactgctc aacaaggcta gtgaccaagt
     3241 cgaacaaata aagtatcaag gtatggatcg aaaacggttg attgatatta ttgagcaaca
     3301 acgtttagaa ttgatccaaa aagaagctgt atgtaaagag tacaaaaaac atttagaaca
     3361 agtaattgag tatcattcag tagaaaagta cagatcagtg gtgcaaaaaa atagagcaac
     3421 gagtcctgat cctcgtcaag atgtatcgat tagtatcaag acgccagtat ttaattctgt
     3481 taaatcagaa aagctagaaa gtatctcaac tgctttggta gaaaaagcag ctgaaatgaa
     3541 ctccctagca aatgaagtta gtcgtcttat ttagtcatat cgataacttg agagtctttg
     3601 gtgattccgt tttttataga attaatggtt tgttcagcgg aatatttagt caagtatgtt
     3661 tcgctagttg ctactacttc attgttattg gacttaataa cgaagtaata ttggccatta
     3721 gttgcttctc ttataacaaa gtacaaattt ttcaccacct ataatttatt tcagcggacc
     3781 actcgctgat aattaaaatt atacgcttag tatttatttt cacaatataa atttgtcact
     3841 gtggcggaaa ggtaacgcta ataacttaaa tcagtggtaa tgagcacctg agcttagttt
     3901 atgcagctag gcaagagacc gtcgagggta aaggacaaga ggcatcttga aataaatccc
     3961 ctaaagatag acaattctag aggtgagagt ttttatgatg gagagtctgt aaaatatcgg
     4021 aaaatggtgt tgcaatgttc gattcattgc cagtgacttt ggtttacggt gatccataac
     4081 tgcctgtcag taaaaccgaa tatacaggtt ggaaactata gcgcgctgat gtttctgtaa
     4141 tccacattcc ctcgaaggat agtgggtcct gtattacata ttagatcact cattgagtgg
     4201 tctttttatt ttgaaaggag ttttatctat gaatgaaaac caattaagag agttatttaa
     4261 aacgaatgaa gcaaaccaaa ccatggaggc gacattctat gaaactcaaa aaagcttagc
     4321 gttaatcgca aaacaagcta agtatttcta cgatcagctt attctacaag ggtttaatga
     4381 aggacaggct atggaattta tgatgcgaac cttttctgcc aataaccaac agagagagtg
     4441 atacataatg agaaactact ggtatgtatc attatcaaat aaatatccgc aaccaaacgc
     4501 agatgatcca attagagttg tccaatcagt ccaaatcaaa aagaagtact ccatcattga
     4561 aatgaccaga gaagctacac cgaaagagat cgataagtac aatcttcgtt actgtggcca
     4621 tggatatttt agtgagcaga acatacagac aaatattgag agatattgtt aataaaattg
     4681 aaaggtggtg gcttgaatgt ggtaaatttg acaccaaaac aaaaagcttt tgcggatgaa
     4741 tatataaaaa atggtggtaa tgctactcaa gccgccatta aagcaggtta tagcaaacga
     4801 tcagcaagag taataggtaa ggaaaaccta actaaaccta acataataca gtatattaat
     4861 gaacggctaa atcctatcga aaagaagcgc aaattaagtg ctgaggacgc tttaaatgaa
     4921 ttgatagata tttggcaagg agaagtacaa ataagcgtga gcaagcaaat agaccgcttg
     4981 gagaaaaaca aggttattaa acatatgcaa tatgaatata caccagattt agaaagcaaa
     5041 ttgaaagcct tggatttgta tttgaagtat aaatcgctgt tatcacaaac gcaattagaa
     5101 aaagctcaaa cagaaataaa attaatgcaa gcaaaattag aacaattaca gataaactca
     5161 gagcgttcta ccgaagaaaa acttgatgag ttgttagaaa agattagtgg tgaattagat
     5221 gggactagtt gatatttata acccaaagca aatcgacgtg ttaaataaaa ccattaagaa
     5281 tgattggttt attactttat tacatggagc aaaacgttct gggaaaacaa aaataaacaa
     5341 tgatttgttc ttgtttgaat tgcgacgtgt gcgaaagatt gccgatgaag aagacattaa
     5401 ggaaccaatg tatatcctag caggagtttc aagtgcaaca atccaaaaga acatcttaca
     5461 ggaactatac aacatgtaca gcatagaacc taaattcgat aaacatggaa actttaagtt
     5521 atttggcgtt aaggtcgtac aagcttatac aggaaacatt ggcggtgttg gtgcaattcg
     5581 tggtatgaca gcatatggcg cttatatcaa tgaggcatca ctagctagac aagaagtatt
     5641 tgctgaaatc gtttcacgtt gttcaggaac aggagcgaga atcctagctg acacaaaccc
     5701 tgacaacccg gaacactggc tgaagaaaga gtatatcgac aattcaagta aaaacattca
     5761 atcgttccac tttgaattgg atgataatac ttttttatct gaacgctata gaaataatat
     5821 taaagaatca acaccaagcg gcatgtttta tgatcgtgat atcaaaggtt tatgggtttc
     5881 tgcagaaggt gtcgtttatc gtgattttga tgctagcaag cactacatcc agtcaaaaga
     5941 cttaccacct ttgagcaact tttattgtgg tgttgactgg ggatatgaac actggggttc
     6001 aatcgtagtt ataggtgaaa cggatgacgg aacagcttat ttaatcgaag aacatgctac
     6061 tcaatttgaa gagattgatt attgggtaga tatagcaaaa ggcattcaag agcgttacgg
     6121 cttacgagtg cctttttatt gtgactctgc gagaccggag catgtggcta ggtttgtaag
     6181 agaaaagatt aatgctaaaa atgctcataa agcacggtta tctggagttg aggaagtcgc
     6241 caagagattt aaacaagata aattgtttat ctgtcaagat agagtgatga aatttcggga
     6301 tgaaatttat caatacattt gggacaagaa aaaaggtgag ccaataaaag aatatgatga
     6361 tgtgctagac tccgttcgat atgcgatata tactcatgag cttcttaaga aaccaaaagt
     6421 taatgtcaac gaaaagatta aacgtgttaa acgtatgttt taaggagtgt gagaaatgga
     6481 taaggtaaac gaatttgaat acggtactga tatacattat tcgaacgacg tgaacacaaa
     6541 ttatgtaaag tttagcgtag aatcaaatct tcactataga tttagctcag cagaagattt
     6601 acttaacgat tcaaatactt tagcagcaat gataaaacat catcatgaat atcaggtaaa
     6661 gcggcttagt gtattagatg attattacaa agctagaaat acaaatatca tggataaccg
     6721 tagacgtaga gaaaaggaaa aagcggatca ccgatcagca cataactttg gaaaagttct
     6781 ttgtacgttt gatgttgggt acaacacagg caatcccata aaagtgcaaa tcgaggatac
     6841 aaatcaacaa aaagaaatcg aagagtttaa tactaataat gacatagatg ggttaaatgc
     6901 tgaactctgg cttgatatgg ataagtatgg gagagcctat gagattatct atcgagattc
     6961 agatgataca gattatgttg atttggctaa tgtatttgaa acatttgttg tatatgatac
     7021 tacagtaaaa cgagagccta ttttggctgt acggtatcct aagacaagat tcaacaagga
     7081 tgctgataaa cagtacattc aaccaatcgt atacacaaaa gaaaaaagta tcacttatga
     7141 tgagacgaca ctaacagcaa ttgagttaaa aaatccccag gatgaaccgc atgaatataa
     7201 agaggtacct attacagagt attctcctaa tcgttttcgg atgggcttgt atgaagatgt
     7261 actatctttg attgatctat acgatgcagg gcagtctgat accgccaact atatgactga
     7321 tctaaacgat gctcttctag ttattagtgg tgatattgaa gcagcaggac tatccacaga
     7381 ggacgccatc aagcagaaag aagcgaatat gcttttgctt gaatctggaa ctgatgtgaa
     7441 cggtaataaa acaagtgtga ctgcaggata tatttacaaa caatatgatg tgaacggtgt
     7501 agaagcatac aaagacagag tacgcaagga tatccacgaa atctcaatgg ttcctgatct
     7561 tactgatgac aatttttccg gagtgcaatc aggagaagcg atgaaatata aattatttgg
     7621 atttgaacaa atgacggcaa caaagcaaag gctattcaaa aagggtctta tgcggcgtta
     7681 tcgtcttttg tttagcctaa aatcaagtat ttctgaaatg gataactccg atttgaaagg
     7741 cttacgtgta atatttacgc ctaatctacc taaagccatt ctggaagagt tgaaatcttt
     7801 ggttgatgct ggagctgaac tcagtcaaga gacgatctta ggactcgctt cttttgttcc
     7861 agatgtacag gcagaattaa aacgtgtgaa tgctgaaacg tcagcggaca gaggtgtgtt
     7921 tgacagtgac aaagaaactg atacggaggt tgaagaagat gaaagcacgt aaaaaaccag
     7981 ttactgtcga gattgtgcaa cttaaaatgc tatctgcacg ttcatatcgc aaatgcaaag
     8041 aattcgttgg tgaagcatgg attgaccata ataatatgcc caatggatta cctggtattg
     8101 aaacattaga aggcacaatg gaaatatctg atggtgatta catcatcaaa ggcgttcatg
     8161 gtgaatttta tccgtgcaaa ccagatatct ttattgagac ctacgaaatc atcgaggagt
     8221 gattaagtga aatcacaaga ttacttcatc aaaagggaga aagcttggca agagcaacag
     8281 attaaagatg acaagaaacg catgaatgag atcaagaagc gtctgcaata cgcgcaggat
     8341 gcgatacaga aagagataga cgcacaatgg aatagttttt caaacgggca gaaaattact
     8401 cgtagcgaag caatgaagcg tgctaatgaa atggatgtaa aagcatttgc tagaaaggct
     8461 aagaagtatg ttaaagagaa agacttctca cctacagcga acaaagaatt gaagctatac
     8521 aatctaacga tgcgtgtcaa tcgactggaa ttgttaaaag ccaatattgg tttagagctt
     8581 atagctacgt ttaacgagct agataagtat ttctcgaatg aacttacaaa agcagggcta
     8641 gacgagctta agcggcaagc tggcatctta gaaatggcga ttgctaaaag cggttatgca
     8701 aaattagtgg agcaagtgat aaacagctcg tttcgagcgg atggatttgc aacgttcagc
     8761 gaaaggctat ggatgtacca agcagagttg aaagcagatt tggataagtt gcttgttcga
     8821 agtgtgacga tgggacgtaa tcctaaacag ctcgctccag aattaagtaa gtttctaaca
     8881 gaagaaggac gagaaaatac taagtttaat acgcaacgtt taatggtaac tgaaacaaca
     8941 agagttcaaa gcggtataca gagggaaagt tacttagatg cgaatattga gaaatatgga
     9001 tgggtaactg aacctaatgc ttgccgatta tgtaagagca ttgccgctga cggtccatat
     9061 ttagtgagtg aaatggaagt aggtaagaat atgatgccag tacatccttt tgatagatgt
     9121 tctacttatc cgattgttga tcgtgatgct tttgagaagt cacttaaaga aagaaggtta
     9181 taacaatgaa gaaagattca tttcaagtta tatctgacag atttttagaa acggcatatg
     9241 ggaaaagcaa agaggaaatt ttgcatttgt atcaaaatga tgctcaattt aaagaagact
     9301 atgatgccgt taaacagcat ttaattgatg tcttagaacc aatcgtaaaa gaagtgtatg
     9361 aaacggctag aaaggttatt gaacctgtcg tgcaatcaat aaccgaattg gttcagtcta
     9421 acccagagta cttcaaagct ttgcaaaaca atatgaatca aagcgtgttc caaagcgctt
     9481 ctatgtagag gagagtacgc atatggaaaa tgaagaagca attgttagag ctatcgcaag
     9541 tttaaataag attctacaaa acgaaaaagt gctaagagta tccgttgaaa ctgatagtga
     9601 atgggaagac ggtattcaat cgattgatat cagcgtgaaa tatttacctt caggagatgt
     9661 aagtgaagcc gaataacggg ctttttatta tgccctctac tgctcggggc aatataaatt
     9721 gagctgttcc gactgaccgg cgtaactggt caaatttatc gggtagcggc gtaaccgtgg
     9781 aggattaatc atgaaaaaac gtttatttat gccaatgaac ttacaatttt tttctgaacc
     9841 aggagatggt ggatctggtg atgagggaca acaaggaaac ctaccagctg gctcacaaga
     9901 gacaccgacc gaagcaaaag aagaaaacaa tactggcaaa acattttctc gtgatgaagt
     9961 agcgaaaatg atcgctgctg agacgaataa agcaaaagca gcgtgggaaa aagaactaga
    10021 agcaaaaaaa gaagaagcta aaaagctggc aaaaatgaat gcggaagaaa aactacagca
    10081 tgagttggaa caaaaagaag ctgaaatcgc tgaattaaag cgtggacagg cactatctga
    10141 aatgacgaaa gaagcttcta aaatgctgac agatgcaaat ttaccacacg atgatgattt
    10201 acttggtctg attgtttctg atgatgcaga tgccacaaaa caagctgtag cagtcatcac
    10261 taactttgct tctttgatta agagagaaaa cgcaagacaa acaccaccaa atgaaggtgg
    10321 acaatttaca gcatcgaaaa atactaaaga aacagtggct aaactagctg ctaaaaatcg
    10381 aattatcaaa taggaggaat aaacaatatg gcacaaacat ggaatccaga taacgtaaca
    10441 gtttatgaaa ctaaagaggg taaaatccct gacaagtata atactttgat tatcaacgac
    10501 attatggaaa actcgaaagt aatgcaattg gcgaaatacg aagaaatgac agataaggaa
    10561 aagaaatttg aatactttgc tgaaggtcct ggcgcatact gggttggtga aggtgagaag
    10621 attcaaacat ctaaacctaa atggttgcaa gcgacaatgg tagctaaaaa gctaggcgtg
    10681 attattcctg tgtctcgtga atacttgcac tacaaaatgt cagatttctt cactgttatg
    10741 caacctaaaa ttgctgaagc tttctacaaa aagtttgatg cggctgcatt actaaacact
    10801 gacaatccgt ttccacagtc tttagaagaa tctgtcgttg cagcggctaa tgttatcaaa
    10861 ggtcctttga cttatgacaa cattttggca ttagaagatg ctttaggcaa aaatgaattt
    10921 gaaccaaatg cgttcatctc aaatcgtaaa aaccgcacag aattgcgttc tgccgctcaa
    10981 acagtcggta caaacgttga atttatctat gaccgctcgg cgaatacgat tgatggtctt
    11041 cctgtggttg acttgaaagc attgggtaaa ggtgaattgt acgctggtga ctttaactat
    11101 atgttctatg gtatcccatt caacatttcg ttcaagattt cagaagatgc gcaattatct
    11161 acgcttaaaa acgaagatgg cactcctgta aacttgtttg aacaagagct ggttgcgttg
    11221 cgtgcaacaa tggatgtcgg ctttatgatc gtcaaagatg ctgcgttcgg taaaattgaa
    11281 ccggcggggg agtaacaatc cccgttatag gcgttactgt aacgcctaaa acctcaagcg
    11341 cagttgcggg gactgctggc aatagacaat taacagctac cgttgcacca caaaacgcaa
    11401 caaataaaac agtgacgtat tcaattgcgc cagtaacaag cggattgacg gtttcttcta
    11461 gcggtaatat cacttggact gacacagtac cagccggtaa atacacgaca acaataaaaa
    11521 cggaagatgg atcacacaca gatactcacg ttttgacact gactgagccg taggaaggat
    11581 aggtacgaat ggtaattaaa gatgacgtta agaagcttct aagcggttct acagatgata
    11641 agctagaagt tatcgagaga cgaactagag agcgcttagc gtcattgcta ggcgtttctg
    11701 ttacaccaga atcactagaa tacatcgttt ttgacgtaac gaacaaacgt tttaaccgaa
    11761 ttgggcaaga gggcatgact tcatacgctc aagagggttt atcgatggct ttccctgatt
    11821 ccgatttttc agaatacgca tcggaaattg atgatttcaa acggaaagat gacgaagaat
    11881 tgtacaaacc tagaaaaggg agcgtgcggt tcttatgaga tttttagatg aggttatctt
    11941 tgaaaaagat ggagaaggaa atcgttacga tccagaatta ggcgagtgga tagaagaagc
    12001 accgattacg gtaacaacaa acgcaaatgt aacggactta ggaacagata gaagtgtggc
    12061 gatttttggg gatatacgac aaggggcgaa ggttatcaga accatgcctc tttttgttgt
    12121 tccaaaatat gaccgcatta tttttaatgg aaagacatac aaagatgtcg ctcaacgcag
    12181 cctgttatca acgaatagca ttatcgtcca ggaggtgatt gcaaagtgaa agtttcattg
    12241 ggttacaaag gtgtggatga gctgcttaaa cacttggaag aagctgtcac gcttagagat
    12301 gtgcaaatgg tagttaaaac aaatggagcc gagcttacta agcgcatgca agaaaaagcc
    12361 cgctttaatg gacattggga aggggatgtg tttgtgcacc cgacaggatt cactagacga
    12421 tcaattcgaa tgtggttgtt ggaaggcggt tttgttgcac aagttggacc gcagagtgat
    12481 tacagtccgt acttagaata tggaactcga ttcatgagcg ctcaaccttt cgttggtccc
    12541 gcatttaatg tgcagaaagc aatctttatg aaagacatgc agaggttatt caaatgatta
    12601 aaacaagaga tcaatcatta tttgatgaaa tgtttgctag gagcctagct cttggatacc
    12661 aaacttacga ctacaaacca ttagacgatg taggttatcc gtttgttgag ttggaaagct
    12721 cccaaacagt ccacaatccg aataaaaccg acatcaaagg aacagtcaat ttgacaattt
    12781 ccgtttgggg attgcagaaa aaacgcaaac aagtgtctga tatggcttcg gcactattta
    12841 atcaagcgtt gcagatacaa gccacagaag gctattattg ggatttaaat tatcaagcaa
    12901 gtggaattca agtgatggac gacacaacaa ccaatacgcc cctaaaacga gcggttgtca
    12961 cacttgaatt tagaattaga taggaggaag aacatggaag cattaaaagg tattgatgtc
    13021 attttgcttt atcgcttatt gaaaaaagaa actcaggaag ctgcttggaa aatggcattt
    13081 caaacagaac acgaaaatgg tttatcaaga gattcagact ctacagtgac aaaagacgga
    13141 aacgttcaaa gtttaagccc agttgaatat gatttttcgg ctacttcaat agttgccaaa
    13201 ggcgattctc atgtagatga aatgaaacaa gccttattaa atggcgatat cattgaaatt
    13261 tgggaaatca acaaagcaga acagggcaca gatgatgatg caaataagta caaagctact
    13321 tattaccaag catatgtgtc tgaatttact ccatcagctg ctgcagagga taacgttgaa
    13381 ttaagtttat catttgcagt aaatggtgtt ggtcaagatg gttatgcaac cttgacagaa
    13441 gatcaagccg atgttgttca atatgcattc aaagataccg tgaaagcaac ttcgacagga
    13501 gcataagagg gcttagatgc tctctttttt attttaggag gatgaaaaac attgaaatta
    13561 aaaattaaag gtaaagaata ttcgtttaaa tttggcacta aatttgtacg tgaattagac
    13621 aaagtgatgc ctttcatcga tggaaatatg gaattcggaa tgggactctc agcaaaagtc
    13681 ttaccggaat tacgttctta taatgccaac acattgtcac gagtcttaga aatagcaaat
    13741 agaacagaag aagaaactat tacgttggat gaaatggatg attacatcga tgaagttaaa
    13801 gacatcgaaa aattgtttga tgaagtccta aaagaattgg cggagtcgaa cgcgggaaag
    13861 ttagcggtcc gaaacctgaa tcagaaattg aaagaagcgg aaaaacaaca agcggaatag
    13921 attctgcact ggcatacgaa caaattctta tcaattcttt tcgatatttg gaaatgacca
    13981 atatctcaga tatcgaaaga atgacgttat atgaatacaa cattcgtatg actgcagccc
    14041 agttatcttg gcttgacaaa gaaaagttga ttcacgaatt agcgtgggca aatcagcaag
    14101 tccaagcgga gaaaaaagta ggcaaaaaga cagttcctgt atatcgatcc tttgaagaat
    14161 tcttcaatta tcaaaaaatc gaagattcaa tcatgggagt ttccgaactt tcaaaacaag
    14221 ataaaaaatt ccaaagctta ctaactaaag ctaactcttg aggaaaggag gaaaatcatg
    14281 gaacaatttt ctgttgaagc cttattaaaa gccacagata gtggatttgt aaagactttt
    14341 aaagatgcgc aagatgctgt taagactttt gaagagaaat caaatagtat gacaaccgct
    14401 gtaggtaaag tgatgcaagg tactggtgcc gcaatgacaa agtatattac cactcctctt
    14461 ataggagtag gcgtagcagc tgctaaagtt ggtggcgact tcgaagaaca aatgagtcgt
    14521 gtaaaagcta tatcaggagc aacaggcgac acatttgaac agatgaaaca gcaagcgatt
    14581 gatctaggag caaaaactgc ttttagcgca aaagaatcgg ctgccggcat ggaaaatctt
    14641 gcttctgctg gatttagcgc acaagaaatc atgaaagcaa tgccgggtct tttagactta
    14701 gcagctgtat ctggagggga tgtggctcta gcttctgaaa atactgctac tgctttgaga
    14761 ggatttggtt tagaagcaag tgaagcagga catgtcgctg atgtatttgc tcgtgctgct
    14821 gcggatacca atgctgaagt tggagacatg ggagaggcat tgaagtatgt tgctcctgta
    14881 gccaattcaa tgggtatttc tttggaagaa actgcagcag ctattggtat tatgagtgac
    14941 gcaggtatta agggttctca agcaggtaca acgttgcgag gagcattgtc taggttagca
    15001 aggccaacaa aggctatgca agatacaatg gataatttag gtgtttcgtt ttatgatgct
    15061 gacggtaaaa tgaaaccttt aaaaactcaa gtagaattac ttaaaaaagc ttttgaaggc
    15121 ctgacgcctg aacaacaaca aaatgcttta gtaacactat atgggcaaga atcattatca
    15181 gggatgatgg ctttgattga taaaggacct gattcattgg gcaaattaac aaaatctctg
    15241 aaagattctg atggtgcagc tgacgatatg gctcggacca tgcaagataa tatgaattct
    15301 tccatcgagc aaatgtttgg agcttttgag tcagcagcta ttgtaattca aaagattcta
    15361 gcaccatcca tcaaaaaagt agcagatgcc atatccggct tagtagaaaa atttgtaagt
    15421 gctccagaat caactcaaag attagtagtg gccataggag caatcgctat tgcaattggg
    15481 ccagtattgt atgcattagg aatgctggtt aaagcgtttc aaaccatgaa agtggggtta
    15541 ggtgtattag gtaacggaat ctctttgttc aagaaattag gttccgccat aggttttctt
    15601 accagtccag tcggattggt tatagctgcg gtagcactac ttgttgtagg tttcatctat
    15661 ctttggaata cgagtgaaga ttttagaaac ttttggattg gcttatggga gggaatcaag
    15721 tctgctgtaa gctcggcagt agaatggatt cagaatgcat ggaaatctac aggagaatgg
    15781 tttaacaatt tatggaagtc cattaaagaa ggcgcagaca atgtttggac tacaattcaa
    15841 gaagcccctg ggaaagcggc agattggatc aagaataaat ggactgaaac aaaagagttc
    15901 ttttcgagta tatgggatgg catcaaagaa gctgccagtt ccgcttggga aggaattgta
    15961 aacattctaa caccgtatgt tattgccata aaaaatgttt ttcagccaat gattgatttc
    16021 tttacgaacc tatggtctca aattggatca atcgcaggtt ctgcatggga aattataaaa
    16081 actgctgtaa tgggtccaat tctacttttg attgatttga taacaggcaa ttttaatcag
    16141 ttaaaagaag atgcttcgat gctgtggact acattaacta caaatatcca aaacattatc
    16201 acgacgtttg tagatatagt tgttggttat tacacagcct taaaggatac tgtgataaat
    16261 atctggaatg tgttgacttc taccatcaaa gatgtgtgga attcttttac tacatggatc
    16321 aaagagacaa ctaacaatat tgtaaatagt attaaacagg ggtggaataa cctaaaacaa
    16381 gggacaatcg atctgtttaa taatatgatt caaggagcga aagatttatg gaattctttc
    16441 aaagcttggt ttattaatct agttattgga actaaggata acatcattca gggatgggaa
    16501 aacctaaaac aaggtactat agatactttc aacaatttag taaatggtgc tcaagaggta
    16561 tgggataatt tagtaaatgc tgttagtgat acggttgata gagtaactgg ctggtttgat
    16621 aacttgaaaa atatcgattt actagcagcc ggaaaagcta tcatggatag ttttctagaa
    16681 gggttacaaa atgcatggaa atctgtgcaa gattttgttg gaggtattgg tgactggatt
    16741 agggaacaca aaggacctat tcggtacgat agaaaattgt taatcccagc tgggcaagca
    16801 atcatgaatg gactaaacgc aggtttaact aatggctttg ccagtgtcca aagcaatgta
    16861 ggaaacatgg ctaatatgat tgcagatagt tttactcgta cacctgatat tgatctttca
    16921 gcgaatttaa aaaatgcaaa tagaaatttc acaacacaaa tagagcatag tgttaactac
    16981 ggcaaaaata aacgtcctgc agttttcaat attcgcttag gaaatcaagt gtttgaagcg
    17041 tttgttgagg atatttcaaa cattcaaggt aaagaggcgg atattaattt attgttctag
    17101 aaagtgaggg aaatatgatg gagtggcata atccaatgta tgaattcaga gacacggtta
    17161 aaaacgacag tcagaaaaca tggataccga cgtctgcatt gaattatgat gggaagttta
    17221 tcgagaatta tatagaaggg taccagactt tgtatgttga aggtagagaa atggtttctt
    17281 tagagattga gagtgaagcg gtgagcatag gtgttcgtat tagttctcaa agacttccag
    17341 agaggatttt aactatacat tttaaattag aagaaaagaa tcctattgaa tttcaacgaa
    17401 gctttaataa attgatgagg ctgctttaca gggataaaga tgtagaaatt cactttaacg
    17461 atgagttgga tatgtattat tacggaaggt atcagacctg tgataatatt ccaggaaacg
    17521 ttcatagtgt aatatctagt ttctctataa tctgttctga tcccagaaaa tatacacgta
    17581 tatttgaaac aaacggcata gtctcagaat atcttccgta tgaagtagct ccgatttcaa
    17641 ttagtttaaa ggctaataat gatgggagct taagaattac aaatggccgt caaaatatta
    17701 gtgtgactaa ctcaatgata aaaaaaggtg actttatcga gatggacata gctgaaggaa
    17761 aagtttttgt gaatggagtg aataaaacga gaattcttga tcttaccagc tcatttaaaa
    17821 actttatggt tagaactggt gatctagtta agtgtgataa tggcactccc ttaatacgat
    17881 atagaggagt gtggctgtaa tggacaaaga tgtttatttt ttcgacgata gccaaaagct
    17941 tattaaaata gtaggggaag ataaactttt ttcggtagtt caagaaaaag aaatcacacc
    18001 aagtaaagat gagttaatta atgataagtt agcagttagc atggagtttg atgacgaaat
    18061 taaagagtca gcttatatgg cggttcgtga aagcgagtcg tctttttcta tgtataaaat
    18121 tatcggaatt gctgatccgg gttcattgtt gatattcact gggattaatt ttgggcctga
    18181 tgaattggat gcttatatca ttaatgatat tcgtccggct aacgaatttt ttcagaaaac
    18241 catccagaga gtcatagatt ttacattagg tgagtggcga gttggtcatt tagattcaac
    18301 tctgccagcg gtttctatga ctttttacta ctgtagtatc cgtgaagcct taaaaaatct
    18361 acaaacgtta ggatgcgaga ttgtttttag gtgcaatcta agtggagaag gaatcacgga
    18421 taaatggata gaagtttaca agcagattgg cgaatacagt aatgagcgat atgagtacgg
    18481 cgataaagcc ttgacaattg aaaaagaagt agatcgaagt aacatctata cttctctaat
    18541 cggccgcggc cgtggtgaag aggttggaga tggttatggt cgtcgaattg agtttgatca
    18601 agtatactgg tccaaatcaa aaggggatcc gttaaacaag cctactggcc agatatattt
    18661 ggaaatccct gagatgactg aaaaatatgg tattcctact aaaaacggaa aacgtcgtaa
    18721 gcgtgagaag gtaattatat tcgaagactg cgaagatcct gttgaactaa ttcagcttac
    18781 ctatcaagaa ttggttaact gttcacgtcc gctagttcaa tttaaagcaa ctatttttgg
    18841 agcagatagt ttaggtaata ttatacgtat tcatcgtgat gaccgcggct atcattacga
    18901 gactagaatt ttcagtgtga agattgatcg attaacagga aaagtcgaaa ctggcttagg
    18961 tgataattta aatacttcat caacacgtca agcttcaaat actcaaactg ccatacagac
    19021 ccttgatgag aagaagatga ccttttatga gtccacagaa gtttctaaat ggcagtcgga
    19081 tatcattcgc ggagcaaagg gtggatcaat tatcatgatg aatccttggg atactggcaa
    19141 aggcgaaagt cgtcaaccat atcagatggt ttggatgaat ggggatagta ttgatacttc
    19201 taaccatttt cttgtagcta attcggaagg gattggtttt attgatggga aattcaatga
    19261 gtcaaatttc aaaacggcat ggacgattga tggaaacttc aatgccaatt atatccaatc
    19321 tggacgtatt agagcagata tttttgaaac ttcatttaac gctgttggtg atcagctcaa
    19381 gttagtaaaa ggagctttgc aaattgtaaa cagcaataaa aaaatcatgg aattaaccaa
    19441 aaaagggatg gagttctgga ataccaaaga atccattggc acaattggta ctactgattc
    19501 tgctggtaat ccttttcctg gagcttctac tcccacacct attgaagata attctttagt
    19561 tattcgtaca aatggagacg gcaaatatat tttgatttct cctaaagttg gtaaaggatt
    19621 agttttatta ggaaatggta aagcaattta ttttggagac ttagatgtac aaggcaaact
    19681 cacagttaac ggaaaagaaa tcacagggaa caacagtgga ggaagtgatc ctggaactat
    19741 ccctcctcaa ttgacgacgg aagctgaaaa aagagcatgg aaaatttgga caatgctgaa
    19801 agctcgtggc tattctgaat atgcagctgc aggtattcta ggaaatattc agggggaggt
    19861 tggagcaagt atgaaccctg atacagaaca acttggtggt ccagcttatg ggattgttca
    19921 atgggatggt tctgcttatc cgttagtcgg atcaccaact tggaacggac gagagtatgt
    19981 tcagcgtttg atgaacaccg cagggattca agaagattat cgaagtattg aagcccaagt
    20041 aaaattatta gattggtgta tgttcaacgg tcaatggctc ggaaaagtaa atccaaccac
    20101 agtatcagga tttaaatcga tcaatgatgc caaaagtgca gcgtatgctt ttgaaatgaa
    20161 cttcgaacgc ccagcttctg cacatccaga acgccaaaat tatgcccaat cttggtataa
    20221 caaattacat ggattaacta gtccagaacc tggagggaat ttcatttgtc caattcaaaa
    20281 accagtgaca gttacttcag aatgtggatg gagaactagt ccaataaatg gcggccaaga
    20341 atttcataat ggaattgatc ttgtaaatgg aaatcctaat acacctgttt ttgcagcatt
    20401 agatggggaa gttgttcagg ctggtgctaa ttattatgac tggtatggca attacgtggt
    20461 tattaaacat aataatggga agtggacagg ttatgctcat ttgtctcgta ttgatgtttc
    20521 tgttggacaa aaagtccaga aaggtgctca aataggcttg atgggaacaa ctggtccatc
    20581 tacaggagaa catctacatt ttcaaattat gaaaaattat tggccacagc cagttgttga
    20641 ttttgagaat ccaagaaatt atatccaatt ttaaggtggt gattctatga gtaaatggaa
    20701 tgtcgtttta agtacaacag aaccatataa ttatgtgggg atgattcaag ttcgacaagg
    20761 caataagaat acagaggtta tggaagcgac tatagttgaa aatggtcttc cctacgattt
    20821 atcagaatgt aaggtatatt ttgaatcagt tgtaggtggg aaatatccag tccaattaga
    20881 aacaaaaatt gtggatgcta aaaaaggaaa aattaactat atttttgata aatattccat
    20941 gcagtgttta catcgacaaa cagccaattt cattatattt aaaggagaag acttgattgg
    21001 aacaactcaa gacttctctt attttgtcat taatgctgtt tcaaaaacag aaggagaaat
    21061 gggttcttat tggcaatcaa tcgaagattt aattgcggat atgacggact ttattaatga
    21121 aaataaaggc gattttacgg actggatgaa tgaaaggaaa gaagaatttg aacgttggag
    21181 acaagaacag gaacacagtt tccaagattg gagagaagga caagaatcag actatttggc
    21241 atggtttgaa tcaatcaagg atattttaaa gacgattgat ccaggtggag tcatgttagc
    21301 cgaactaacg gatgcgcgag tagatattca aggagttcgt catgcgtcta tttcagagcg
    21361 tttgctggca gatatggact atttatatca gaaattgcga gcaacgcttt tcacgattga
    21421 atacggggag attgaagtga ctgatatttt gcaggatgat ctcttttcag ataatcatga
    21481 agtcgaaaaa gttggaactg tagcattccc gattgaagaa ggagccttga tcatcgcaac
    21541 cgttgatgat ccaaagcaaa atgtgttcac tcttgagaaa gttggggtga tctaatggct
    21601 aaaactaaac gaatgatgga aacggatgaa aaaacgggtg tacaacgcca attctttcca
    21661 atcacacatg cttccgcagt tcttgggtta gaagaaataa tagcaggaga agcaacagtt
    21721 ttatctgtta atggaaaaat tggagccgtc gtcattacta aagaagattt gggattagag
    21781 aatgttctca cagaattacc ctatgcaagt gaagaagatg acggcattat cacggcagaa
    21841 atgtatcaaa aaattttaaa cagtggagaa ggtgactacg tgttgccagt tgccactatc
    21901 gaccgtttgg gtggcataaa agttggtgaa ttattgacga ttgatgagac agggaaagtc
    21961 tctgcagtca gacaatctga tgtcaatttt tcgctggagt taaaagaaaa actcgattca
    22021 ctgaaaaatt atactgctgg agaaaacatc actattgatg aagatggaac aattagttct
    22081 acaggtggta gtgggactgg tggtgtcaat cagtcatatg tagatcaaaa attccaagaa
    22141 gctgtaaatc aagcagaaaa ttacacaaac gaaagaattc caaattttac ttttgaaaaa
    22201 attggggagg tatagagaat gacagatatc gtaaaaataa aacaaagcaa tgtacaggtt
    22261 tatccacaaa ctcattggaa tgctatagaa ggcaaaccaa caacggtgaa gggagataaa
    22321 ggcgatcctg gccaagcagc aacaattacc ataggaaccg tttctagtgg ttcaaccgct
    22381 tctgttacga atgtaggaac atcatctgca gctagattta attttgtttt accaaaggga
    22441 gataaaggtg atcctggaat aaatgccaca actacagctg tagctacaac tacagcaaat
    22501 gggttgatgt cgtccacaga taaaactaag ttggatggaa ttgcagctgg agcacagaaa
    22561 aatccaggta atgctacaac tacgacagca ggtttaatgt cagcaaccga taaagtgaaa
    22621 cttgatggat tagccaatat tacatttgag aaagtgggga cggtttaatg gctgatattg
    22681 ttcaattaaa agaagacgga gttgctaaat acttaaaaac gcatgtaaaa gctattgatg
    22741 gtaaagaagc attggtgcag acagatggag accaagccat tgcaggacat aagaattttt
    22801 caggttctgt aacaatcaat aataaacgtg ttttgacgac tgatgataac agatatgaag
    22861 tggtaaatct ggttgtcact aacggtaata caggg
//
LOCUS       DAHUVV010000036        22356 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.036, whole genome shotgun sequence.
ACCESSION   DAHUVV010000036 DAHUVV010000000
VERSION     DAHUVV010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.036"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(110..628)
                     /locus_tag="NK767_001411"
     CDS             complement(110..628)
                     /locus_tag="NK767_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCH6694413.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(630..1247)
                     /locus_tag="NK767_001412"
     CDS             complement(630..1247)
                     /locus_tag="NK767_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCH6694414.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1244..2098)
                     /locus_tag="NK767_001413"
     CDS             complement(1244..2098)
                     /locus_tag="NK767_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6694415.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2095..3006)
                     /locus_tag="NK767_001414"
     CDS             complement(2095..3006)
                     /locus_tag="NK767_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6694416.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3047..4381)
                     /locus_tag="NK767_001415"
     CDS             complement(3047..4381)
                     /locus_tag="NK767_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCH6694417.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4589..5419
                     /locus_tag="NK767_001416"
     CDS             4589..5419
                     /locus_tag="NK767_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HCH6694418.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5663..5981)
                     /locus_tag="NK767_001417"
                     /pseudo
     CDS             complement(5663..5981)
                     /locus_tag="NK767_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6103..6297)
                     /locus_tag="NK767_001418"
     CDS             complement(6103..6297)
                     /locus_tag="NK767_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694419.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6802..6987)
                     /locus_tag="NK767_001419"
     CDS             complement(6802..6987)
                     /locus_tag="NK767_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694420.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7837..8178)
                     /locus_tag="NK767_001420"
     CDS             complement(7837..8178)
                     /locus_tag="NK767_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCH6694421.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8178..8408)
                     /locus_tag="NK767_001421"
     CDS             complement(8178..8408)
                     /locus_tag="NK767_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCH6694422.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8695..9033
                     /locus_tag="NK767_001422"
     CDS             8695..9033
                     /locus_tag="NK767_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694423.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9238..9477)
                     /locus_tag="NK767_001423"
     CDS             complement(9238..9477)
                     /locus_tag="NK767_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCH6694424.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(9453..9800)
                     /locus_tag="NK767_001424"
     CDS             complement(9453..9800)
                     /locus_tag="NK767_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCH6694425.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(10532..11113)
                     /locus_tag="NK767_001425"
     CDS             complement(10532..11113)
                     /locus_tag="NK767_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCH6694426.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11296..11922)
                     /locus_tag="NK767_001426"
     CDS             complement(11296..11922)
                     /locus_tag="NK767_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCH6694427.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(11944..13275)
                     /locus_tag="NK767_001427"
     CDS             complement(11944..13275)
                     /locus_tag="NK767_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCH6694428.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(13430..13831)
                     /locus_tag="NK767_001428"
     CDS             complement(13430..13831)
                     /locus_tag="NK767_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCH6694429.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(13917..14123)
                     /locus_tag="NK767_001429"
     CDS             complement(13917..14123)
                     /locus_tag="NK767_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694430.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(14218..15720)
                     /locus_tag="NK767_001430"
     CDS             complement(14218..15720)
                     /locus_tag="NK767_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HCH6694431.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(15738..16193)
                     /gene="argR"
                     /locus_tag="NK767_001431"
     CDS             complement(15738..16193)
                     /gene="argR"
                     /locus_tag="NK767_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCH6694432.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(16291..16908)
                     /locus_tag="NK767_001432"
     CDS             complement(16291..16908)
                     /locus_tag="NK767_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCH6694433.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            17129..18502
                     /locus_tag="NK767_001433"
     CDS             17129..18502
                     /locus_tag="NK767_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6694434.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            18577..19689
                     /locus_tag="NK767_001434"
     CDS             18577..19689
                     /locus_tag="NK767_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCH6694435.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(19761..21398)
                     /locus_tag="NK767_001435"
     CDS             complement(19761..21398)
                     /locus_tag="NK767_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCH6694436.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(21566..22174)
                     /locus_tag="NK767_001436"
     CDS             complement(21566..22174)
                     /locus_tag="NK767_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCH6694437.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         6540 a         4545 c         3885 g         7386 t
ORIGIN      
        1 aaaacttaag ttattaagac aaatgaataa acaactacgc tttcctcgaa acaaaaaaat
       61 aaagcacaaa tgcgcattta cgcactcatg ctttattcgt acaattaatt cagataacgg
      121 cagcgcatag gatgccaata acacttccta aaatagctcc tcctaaaatg tcagtaggat
      181 aatgtaaaag taaaaaaatc ctcgaataag cgatcaaaca agcaaataaa aaggctaata
      241 ttcctgcaac tggacttaat tcgaacaacg cagctactga taaaaaggct agcgctgtat
      301 gtcctgaagg aaaagaagaa gtcttcggtt tacttgtgta aaggacaagc cgacgatttt
      361 tttcataagg tcttttccgt cttgtcgttt ttttgataaa aagcaaacca acaaaaaaat
      421 agatcaaact gccgataaga atggctagac catattctct agtatcaggt atcaagacta
      481 aaacagcagc aaggagcaac caaataaaac caccattaga gatattactg ataaatttca
      541 aaaaattgat ttttcgacta tccgttttcc gttgcttggc gaacttgttg ataatactat
      601 catcgatttt agcaatcaaa cgcaacatgt tattcctcct ttttaagaac cttttcttgc
      661 gtacgatgga taaaaagcag gacttgaaaa taaaacaaga aactgaaacc taataaagca
      721 aaatagattg gtcctgcaaa ccagaatgcg acaagaagga tcaacgtgcc tgccaaatag
      781 aaaagcgcgc ttttgaaatc attaaaaaac aagaaaaatg cagtaatcca agaagcttct
      841 ttttgttcca gttgaagcaa gattttgtaa ctaaagagaa gcgcgcttaa tagaagcaaa
      901 aacaacgtag tatacatcaa tgtatagcca attcccccac cccaatagcg gaaaatctga
      961 taatcgatca atagaagaag aaaataaaca cttgctaaac tatcttgctt tccataaaga
     1021 gagaagttct cacgaaactt tttccagaat agtgaaccga gctttttctc ttcatttttt
     1081 ttgtaatgat aaatcatata cagaactgtt cgacttgcag ttcctaatgt gaaaacaggc
     1141 aagacgcaca ggaaccatag tagctgtaaa tggataagac gtggaatcca ttcaatgatt
     1201 tctaagaaat aatcattggc ttttttacca atcgatcgat tcatcatcct tttgaacctc
     1261 ctgtcaaatc catcccttca atgaaataac gttgtgcaaa aaagaacagc aaaatcgtcg
     1321 ggatcaagac aagtgttgct cctgccatca gataattcca ttgtgtcgtt gcttggttct
     1381 gaaaaacttg caagccaatc tgtaacgtgt atttttcttg atcttggata tataacagag
     1441 gacctaagaa atcattccaa gctgcgttga aggaattgat cgcaatggta atcaaggcag
     1501 gtttcgtcaa tggaagcatc aaatgactcc aaatatacaa atgattcgct ccgtcgattt
     1561 cagcagcttc gatcaattca ttattgatcg ataagtaaaa ttgacgcatc agaaatatat
     1621 tgaacgcatt gccgaagaac gatggaacga tcaggggcaa ataagtacct acccagccga
     1681 tcttggagaa taatacatat tgagggatca tcgttacaaa ccccgggatc atcatcgtag
     1741 aaagaaccaa agcaaacatc agctttttcc caggaaaatc aagcttcgca aatccataag
     1801 caataaaaga atttgaaagg acattaccga tcactactag cacagtgatg aataatgtat
     1861 ttcttgcata gcgccaaaaa ggaaaggcgg caatcgtatc cagatagttt tcccatacga
     1921 ttttttctgg gaaaaaggtc ggaggataac gaacgatttc ttccattggt ttcaatgaag
     1981 taaacaccat ccaaagcaat ggaatgatca aaatgattcc tattactgtc atgaggacat
     2041 aacttagtat tttttttgtt tgccgattca cactggtctt tttgaaataa tccatcattt
     2101 gccgtcacct ccagaataat agacccactt aggtgcaagc ttcaaattga caaacgtcag
     2161 aaccaacacg atcagaaaca ggatcattga catagccatc gcataaccca tatcaaaagc
     2221 tacgaacgcc ttgttccaca tatgcagatt ataaaaaagc aaagaatttg caggtccgcc
     2281 tgttccattt ttcgtcatga cataagcttc ttgaaaaatt tggaaagaac cgatcgtaga
     2341 ggtgatcaca tcgtaaaaaa taataggagt gattaatggc aaagtaatac ttttgaactt
     2401 tccccaaacg cctgctccat cgatttctgc tgattcatat aaagatctag gaacattttg
     2461 taatgtagct aagtaaagta gcattgctcc tccagaactc catattttca tcaaaatcaa
     2521 cgcaggtttc gtccattgtg gatcaaacag ccaagctggt ccagggatat ggaaccagcc
     2581 cagaaatgtg ttgatcaatc ctgcagatgg agaaagcagc tgcatccata agatgtagac
     2641 tgctactcct gataagactg ccggtaaata gaaagctgta cggaatacac tgattccttt
     2701 gattttttga ttgagtactg tggctaaaaa cacactgaaa atcgtcgtca gcggtacatt
     2761 gaagatgacg aaataaacgg tgttgtataa cgatttccaa aataaatcat cgtgaacgaa
     2821 cattcttttg aaatttgcta gaccaatgaa attcatcttg gatgtcaaat tataatcggt
     2881 gaaactacca ataactgaaa aaagcatagg ccctagcata aataccaaga acccgataat
     2941 aaaaggcgag ataaataaat aggcccatct tgcttctttt ttctttcgga tagataaacg
     3001 cttcaaattt tcttgcctcc tctgtaccta gacagtcaaa aatggattat tgattttgct
     3061 tgactaagtt ttctactgct ttttgtgcat ctgccaatcc ttcttttggt gattttgttc
     3121 ctaaaagaat ctggtcaatc tgttcattca ctaatgtcgt gtaatccgga gcatctaaag
     3181 gaactggtgt gatcaccgta tttttaaagt tttcgtccgc catttgatag atcatttttc
     3241 ctttatccgt taggttgtca ttattcacta aattctcatt ggctgtcttg ctcgccatga
     3301 tatcaaaact tttttcgccg aatttttctt gtacttctgg agaagaaaga tatttgatga
     3361 attcataaga ggcctcagga tcttttgctc cgtatggtac ttccaagacg aatccgccac
     3421 cccacgacca atggcctgaa ccttcttctt tttctgggag ctgcatcacg ccaaaatcaa
     3481 aatcatccgg tgcattttcc atcaaactag agtaataatt gatattttgg gcacgcatgg
     3541 ctactaatcc ggaaataaat ggatcagata caccagaccc aaattcggct tctaatttat
     3601 tgatcgtatt tttcccataa taatcttgcc agtcttggat ccactccaag gcttccactt
     3661 tatttttagt atcaatcttg actttttcat ctttgtcaaa ccaactagtc ccgttatctg
     3721 cgtttagcgc ccatacatca gcccctaaat tccataatgg ataaaaaccg atgcgtgtaa
     3781 attcttttcc ttctttttta tctagtttac gtgctgtttc ttctaattca tcccaagttg
     3841 tcggtagatc ttgttcactg attccagctt ctttaaataa ctttttatta taaaaaatga
     3901 cttgtgtatc tgtattgaat gggatcgcat aaggatcacc ttcatattct actgtatccc
     3961 aaagctgcgg ataaaaatcc ttagaagtgt ctttttctac atatttggag agattagttg
     4021 cttgctgtgc ttctgctctt tgggcaacgg aatttatgtc ctgtacaata acatcgggtg
     4081 catttccagc tgttattgca gataaagatt tagtccagat gtcaccccat ggctgatact
     4141 tataatttac atggatctcg ttttgtgatt gattgaaatc ctcaatgatt tcttcgatag
     4201 cttcttttcg tgcaccggat ccccaaaagg accagaaatc aatatttttt cttccatcac
     4261 ttgttttttc agaagaattt gctgcacttg aactggcatc gcttgcatta cttgatccgc
     4321 atcctcctaa taaaaacaat gctgaacttg ccagcaaccc tgtaaacaat aattttttca
     4381 tattttacct ccaaatttta atgtaaccga tgccggtttt aatataacga tatcattttg
     4441 ttctgaaaat aacaatttat aaggtatgat tctaaaaaaa ttattaatga taccaatact
     4501 ttatataatt cgcgagcgct ttctttgaat tttttaatct ttcgtagcag aatgagaatg
     4561 tattaaaaat agattaggag gaattttgat gtttagacac cagaaagaat tgcagtttga
     4621 agcaaaacca gatcgtccga atccattaat cgcgaaatat ctgcaggagc tgatcggcgg
     4681 ccaatatggc gagatgagtg tagctatgca gtacctgttc caaggctgga gctgccgagg
     4741 agaagaaaaa tataaagata tgctgatgga cattgcaaca gaagaaatcg gccacgtgga
     4801 aatgttggcg acaatggttg ctcgtttatt agagggtgcg cctgttgaag atcaagaagc
     4861 agcgatgcaa aaagatccaa caatcggtgc cattatctct ggaatgaatc cacagcacgc
     4921 gatcgtagct ggcttaggac ctcgtcctgc tgacagcgtg ggcaatccat ggagcggtgc
     4981 ctatatcatt gcgagtggca acttgttagc agactttaga gctaatttaa atgccgaatc
     5041 acaaggaaga cttcaagtag cacgtattta cgaaatgatc gatgatcgtg gcgtaaaaga
     5101 tatgctgtct gttctccttg caagagatag ctatcatcaa aatctatggg aatgtgcagt
     5161 gaaagaatta gaagaaaaag aaggttcaat cttagtccca agtactttcc cgcgtgacaa
     5221 agaacgaaaa gacattgctt atgacttcta taacttctca gagggagacc aaagtagtga
     5281 aggttcttgg gctagtggca aagcactgga tggcgaaggc gaatatcaat ggttgaaaga
     5341 acctaagatc gaaggcgctg caccaaaatt agaaccagta acaccaaaaa tgtatgggac
     5401 acctccattc aagaaataaa tatctacccg tagaaacacc cgttttttgt tccacacgct
     5461 ttgtgagcaa aactgactaa agtcattaat cataaccacc cttcaaaagg gtggttttct
     5521 ctagagctat aagctcaaat caccggccag cgcctaaaga cgctggcttt catattttat
     5581 gttcaagcca tattgccttt gccagttttg cccacctctt aaaaggttta tgtactactc
     5641 cttagccctt aaaaggatcg tcttactctc gtacactcag cttatctaac gcactatcat
     5701 gtttttcttg ttcttgaatg tactttttga tggtcgcttc attttgtcct acttttgtca
     5761 catagtatcc ttccacccaa aagtgtcgat acccaaattt atattttaga tttgcacaat
     5821 ctcttgatga tctcctgaat attggctcga tattgattat aaattatttt tcttctatac
     5881 tttggtgtga atacaatgtg gtatttacac atctactttg tacgggctaa actatttccc
     5941 tttattgctc cacccgcata gcaagtggtt ttgtatttca atcgctcggc gcgctcaatt
     6001 aggtctaaag acaataaatc taaaaacccg ctgcttcact gacagaaaca acgggttttc
     6061 agaaaaaaat gaaatgaaaa agatctctta cggcaatcta ttttattttt ttgatttttt
     6121 ataaatggaa aatgcttcaa gaagtccaag aaataaagcc gtataaaaac aagaacctat
     6181 gaaatcttta tttattagat attcaacaga aatcccgata acactagcca acaatatatt
     6241 tatcagaagt gtaaaataag gatgcttttt atagaataga actagtgttg ttttcataga
     6301 ctctcctttc ttcatatttc caattaatca caaattcacg gcattactag aaaaattaaa
     6361 ggggcgcaga agtggaaaaa tactcttcga ctttccctac aaaccaggcc atcttgtgtt
     6421 taataactat accaaatcta aggtacctct aaatctaaat tccatttgtg gcggtgacct
     6481 ccaattttta actcttctat ctcccatcca gtatacataa ccttgatact tcgaccactt
     6541 atatatcgaa cacacaaata cctatccatt gcaggagtac tacctaactt agttgctact
     6601 tgcatctaaa taacagctga aaagatagga cttactctta gtaaagtatc tgaactatta
     6661 atttttaaac tatcattttt atcctcaact acatgcacat ctgttgcaac ttcactttct
     6721 gtctcatcca gataaatgat taaggaatcg ggaattgcaa tatcttcagc tgacgctgga
     6781 atagttgagc taaattagtc atcatatctt tattataaaa taaccaacag caaaaaatac
     6841 aatgaaaaac aaacttggaa ctaatgtatc attaataaat gttgaaaaat tcagcaactc
     6901 attactgtat gattttagca gcccaaccaa catataccct ataaaagcta ataagatggt
     6961 taggaatcct ttagaatttt tactcattca tagacacttc cttaaataac ttttattata
     7021 gaaggtattg taaataataa tattgacaaa aagtgactca aaataaaaaa aaacttacat
     7081 tatactggaa tgaactgtat tgatagtgta cctaaaagct ctactgacat aagatcgtgt
     7141 aatcggtaaa gtaattttat taattatacg ggatgtaatc aattagagaa taaactaatc
     7201 ataaaaggta cttttacatt cataattgtc gctttgagat tctactttgg ttcactatct
     7261 gacccaattg cacattttcg atcattacaa gtattggtgt cgttagaaca actaaataaa
     7321 aactagcggt agatttttca gaatctatgc taatttctgt atggatgttc cagtgatggc
     7381 aaatttctct tttcattgtt tttcttgtcg catgatatgc ctttttgtgt tattcagtta
     7441 aactatagaa attctttcct agacagatct tgatcccaaa ttttacgttt ttgagcatcg
     7501 atatagttta aaatgacatt actcatatct aaagagttta gattttttag gactaaaact
     7561 gtcagttgtt ctttttcttc tttctgttaa agtattgtcg cgacttttcg tatacgttaa
     7621 gatatgtcgt gaaaaaacaa gttttgtgac acaaaaaaac gaatgacttg aggaaatcac
     7681 ctcaatcatt cgtttaaaat gctatatatt ctttagttta agatacggaa attaatgcca
     7741 tcgaccacac tagtagaatg aacgacattt acttggcctt tttttaattt cgttatgttt
     7801 agcatataaa aatattatag agtttactta caatgcttaa aacatatact gtataatctg
     7861 atcaatttta gccatgtctt gcaatggtaa gttttctatt tttttgagtt ttcgttcctt
     7921 aaaatcaaga gattttaatt gtgtaataat tacttgtcct tttatatcta gctcttcagg
     7981 taatgagtaa cgtgtcggta aattctttat agtggaagta attggacata tgaccgaaaa
     8041 cattgtttta cggttaaatt cataacgaga gacgactagt cctggtcgtc ttttttgaat
     8101 ttcttttcct acggagggat caaaatcaat ccatacaata tcaccttttt taggaatata
     8161 aattctttct tctgacacta taaaatttct ctcccttcag gagttaaatc ttcccattcg
     8221 tctttctcat aatactcggc ctcttcccca ccactaaatg gatcttcaat ttttggaact
     8281 aacgtaattg ttccatcatc agaatacaca acgatatatt cttggttttc tgaaggtttc
     8341 tttccgttat cagaaggtaa agttaccact acagaacttc cttgtaatct agattttgta
     8401 acaagcatta tcatcactcc cttaataaaa gtatactata taacactaag taacgcaaaa
     8461 ttcccctttg taaactatct agaaaattgt tttgtttaaa gtatatctta ttggatataa
     8521 atagaaataa attgtagcca gttcattttt tctttaacta aaaaaataat ccaagaaaca
     8581 ttgaacctat ggatccaaaa gctacccaaa tactatagct aaccccccgc taccttcaat
     8641 tttgcagata aaacgctttg ggagaaaatt tcaaagacaa caggccatcc tatcatgccc
     8701 gattataaac aaaaagctgg ctattccttt tgctctgaca aatttcaatt gggaaagttc
     8761 acgaaactag caggtgaaga agtttcaact gttcggatca aagaatgccc gcttcaaata
     8821 gaaacattgg ttcaacgaat ttcaactaga caagactttg ctattgtaga acgtgagatc
     8881 gtagctattt ttgtagaaga agggatttta catgatccaa ctcatatcaa tgtggataaa
     8941 tggcaacctc ttatctataa attcagagaa tatacaactg ccaaccagtc gttaactttg
     9001 aattttagat tccaagaatt caaagagtca taatgataaa catcgttagg tataaataaa
     9061 aaagactcgc agcacgagta aaaaacaaat agaagctata cattagaaag gtcgactgtg
     9121 accctataga tgcagtatat gcagcttatg agcatttctt ctgttctctt tgtttttggg
     9181 atctgtgctg aagtcttttt cgttttttgc cgctctttct ttgaaaaatc gattatgtta
     9241 taccttctcc attactaatt caggcatctg ctgagtaatg atttttcgcg caaaagaaat
     9301 atctgttacc caaggttcga tttgttcttc agtcaagaca agcggcattc ggtcatggat
     9361 tggtgcgact gtgcggttag gttcagtagt cattatgatt gattctgcca ttttttcatc
     9421 ctgatggcta cgataaaaac cgccaatata cactattttt tccttatttg aaaacataaa
     9481 tttgtctttt tcttcattcc actcattggg aaaacacaac gattttgcaa aaatggttga
     9541 tgaaaggtct ttttttcttc tactgtctct gcacgggcat taatcatcaa ttgtcctttt
     9601 ttaaatccgg taaatcccca cttcatgatt tccggagtga ggatgttttc ttcctctttt
     9661 cttaacgtca caacaagatt tgacggaaaa acttctccag ctgctaattc tgctgcctgc
     9721 ttcggttgtt tcgtctgggc ttctcgataa taattctgaa ggagctctga ctctagatca
     9781 aaaaaataac gaccacacat cctaatccct cccaatcttt ttttctatca tacactgcaa
     9841 caagaagttc gcaccaacaa aacgctttca atttttgtag aactaaaaaa gttgtgacaa
     9901 gtgacaaact ctccagtgaa tggtattctc ctgccaactc ctcggtcaca agtcacaata
     9961 ttcaacaaac atgagaagct gctttttgaa attgttcctt gtgactcgga gtgaaacggc
    10021 aggcttacga cctctgggta aacggtgttc aaaagccagt ttctcggtat taagccaaat
    10081 ttatgcaaaa atgtagaaag ctattttcac aaaatttttc ttaatactcg aaactaagcg
    10141 actttttcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    10201 caaccaaacg aaaaatatga gcagctattt ttatttggct gttcttatta cttgatgcag
    10261 ggcagctttt tcacaacctc tccactaaac gatgttcaat cagctaacct tcggtattag
    10321 ctcaaaaaac ggcaaaatat gaggaactat tttgccgttt gtttctctaa tactcaggtc
    10381 aaagcgctga tttcacaacc tctaatacac ggtgttccag aagtagcttc ttcgaaaaat
    10441 aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
    10501 agctgaccac ttctgtcaca acctctaatt attaaagaac cttttgcgaa gttaacaatc
    10561 tttctttgat caattgaaaa ttctgcctaa gtagtgatag atcctgtgtg actttcaaca
    10621 ataagatact tccctggatt tcagcaatca acgctttggc ttgacggtct gcttcttcat
    10681 cggacagttt ttcttcagaa agtgccagga tattttgttt cgtatacttg acccattgat
    10741 tgactaattc tagtagcaac agacggtatt cttcatccac tgtactcaac tcaataatca
    10801 aattacctac aggacagcca tgataagtga tctgtgtctc atgatcagcg aaaatccagt
    10861 ccaacatttc ttctaatgct tcttctggct gttttccagc atttaagatg tcttcgaaca
    10921 attctgtctg ccatacgtcc atatgcgcat ggatcaccgc tagacaaact tctttttttg
    10981 aagaaaagta ataataaaat tgaccttttc ctgtatcaga agctgctaga atatctcgga
    11041 ttgacgtttc atgatagcct tttttataaa taagatcttt ggtcgcttcc aagatctttt
    11101 cctttgtttt caatgatatt ctcctttttc atttctcata gtcctatatt atacagtaaa
    11161 ttcatcattt tttcttctta atcgtttaaa aaagactgtg ccaaattatc tgacacagcc
    11221 ctcactataa aactgttctc atatagctag atgttcattg attcaagaac tgcgaggaca
    11281 aaagcaattc ctatcttatg atagttgctc taatacttcc tctgttgtca tcacttgatc
    11341 tgcaataaag ccaaagttcg tcaacgctgc atccattgct tcttggccag gagcaccaat
    11401 tgcatctgtc gctaccacaa cattgaatcc actctctacc aattcacgca tatgggaatc
    11461 gctacacaag tttccgttca ttccaccaag aattactgtg tcgattccat tttttctcaa
    11521 ttggagcgcc aaatcattgc tttctggtcc aaagatttta tgaggagagc tgatgatcgt
    11581 attttcatcc aaataaggtt tcaattcttc gataaaatcg gctcctgaac cttcttccac
    11641 tgattcatat tgactttttc tagcaaacat ctgctgctca agcatcatat gttctcctgc
    11701 accgccaaat tgccatttat ggtcatgtgg gtaaaaataa tgaggagaaa taaagagttt
    11761 gtatcctttt tcttttgctt gttttaccat ttcaatcata ttttcgatcg tattgtattt
    11821 ttctaagata tcttttgtca aaccaaagcc accgcctgct ggtttcaaga actcgacttg
    11881 cgggtcagtg agaactactg ctgtattttt attattgaac atggtggatt ctccattcgt
    11941 tatttatttt aatacatcat ttagagcttc actcattgtc ggatgagaat agatttgatc
    12001 tctcaataac gtataaggaa gttttccatt catagctagc gaaatcaagt tgatgacttc
    12061 atgagaatct tccgcataga tacttgcccc tagaatcaag tcagtttcag ggtcgactaa
    12121 aactttgaat acacctttag gatcttctaa tacttgtgct ttagggacac cgcttgtcat
    12181 atacttgaat aatttgtaat tgacgccttg tgctttggct tgtttttctg ttaaacctac
    12241 attggataaa gtcggagtga taaatacact gtaaggcaca gctttccggt tattcgttgt
    12301 tcgtttattc tcgcccttca actgatccat aatgattcga taatcatcta gagagatata
    12361 agtgaactgc aatccgcctt tgacgtctcc aatcgcccaa atgttgtctg catctgtacg
    12421 aaggtattca tccacagcga ttgctccgcg atctgttagt ttaacatctg tattttctaa
    12481 tccaagtgat gctgtacttg gttttcttcc agttgcagcc aaaacttttg acgcttggat
    12541 tgttacttct tggccatctt ctgtaaaggt gacggcagct gttgattctt ggtctgctac
    12601 ttgatctaca gatacaccta gatggaattc gattcccgca tttgtcatgt cttctaaaat
    12661 catttgcgcg atatcttcat cttcacgtgg aagaaattca gaatgggcat caagaacgac
    12721 aactttagat ccgtattcat tgaacattga tgcaaattcc aagccaatat atccagcccc
    12781 gatgatcact aatttttccg gtaattgttc ctgatccatc gcttgcgtag aatcaatcaa
    12841 gtagcgactt gtattcaatc ctttgattgg taaaatgatt ggttcagaac cagtattgat
    12901 gaaaatccgt tcgccttcaa caatcacttt ttcaccatta ttcgtcactt cgatttgatg
    12961 gttagaaaga aaacgtgctg taccgtccat cactgtccca gttgcctcat cgctgatcat
    13021 atgataattt ttggcgcgaa gcatgcccgt caatttttct ttccgagcaa cagcctctgt
    13081 aaagtcgatt cctctttctc cattgaaaat caatgattta gaaggaatac agccgatgtt
    13141 gatacatgtt ccgccataca tacgtgttga cttttcaacg actaaaaccg attcaccttt
    13201 tgaagctaac gccttcgcta aagtcttacc gccctttcca aaaccgatca caatattttg
    13261 atatttttcc atcatttttc cctcgcttct tttttaccta ccgattggta caaaaatatg
    13321 ataaccgata attgtctatt ctccaaatca tctgctttat ttgtcaaaaa aaaccagaca
    13381 aaaagcggtc ctttcacgtt tttagtctgg ttcgatttcc tttattcttc taaacaatcg
    13441 ttacttctac atcgatattt cctctagttg ctttggagta agggcagaat tcatgcgcat
    13501 cttctgcaat tttttgggct tcttcttcgc tgatgccatc gatatgcact tctaatacta
    13561 caccgatctg atagccgcca tcatctaaat tataaagaga gacacgtgct tttactgtgg
    13621 actctgctgt cattcctgct tctttgatca tatgttgtac cgccccattg aaacaagaag
    13681 cataacctgc tgcaaataat tgttctggat ttgttccttc tgcatggatt cctggaggag
    13741 tgattttcat atgcatcgag ccatttggtg cctcgacttc tccattgcgc ccgccatggt
    13801 tgatcattgc tgtttcgtat gcttttttca ttgtactgct cctttcgatt gaccatttgc
    13861 tacttggcta taattatagt gtatgacaag taaggcgctt tctacaaaaa ttctgcttat
    13921 tcgaatacca gaatgccgtc ttctactatt tttccaaaaa acaaaaaaat actactcgtg
    13981 aacaacagcg tcatatttgt tccaaaataa atagcaatct tttctacata atcttttata
    14041 tgattgattt taagatacat atataccaga aagttgaccc cataaaccaa tactgttaaa
    14101 aaaataacca tattcgttac cattttttcc tccttttgct ggtctcccta ccagcactcg
    14161 atttaaccat accacttttt ttgccgtcca agcaactagc ctttcaaaaa gactggttta
    14221 ttctgctgtt aatgattcaa gatcagaaaa atgtccgaat aattttgcta attttactaa
    14281 cgccaacttg ttctttccga attcatcatt ttctatctgt aatactagta acggctcatg
    14341 taagcttaaa cggatcaaaa accagccgtt tccgtatggc tcttctagat catatcgaat
    14401 accttcttca ttttccgggt cgaagttcat ccctttttct tcttccaaca gtcgcttcat
    14461 ttcttctaat atcttgtttc cttgttcgcg tggttcatcg ctgaacaatt ttaatcggat
    14521 ttcttgtact tctacgggct gtttcaactc tttgatttgt tcatctaatg tgataccttt
    14581 tctttttaat tctggcaaaa gcatcaggat ctttgcgatg acatatgcac catcatccag
    14641 aaaatagttt tccttgaatg cagcgtgtcc gcttgtttcg atggctagct gtgtatcttt
    14701 cccaagacgg ttaagttcta atgctttatt gattacattt cgatagccgg aaatataacg
    14761 aacttggatg cctccttttt cttcaatgaa tctttgtaga tgagaagaag taggggaatt
    14821 cgtcacgatt cttgttcctg gatgctcttt taatacgatt gtacttaata ctgcgatcaa
    14881 attatttcga ttgatcactg ttccagaagc actgacaact gcagaacgat ccacatctgt
    14941 atcgaaaatg atcccaagat ctgcttcatt tgctaaaaca gcctgcttga tactttccat
    15001 cgcttcttta ttatcaggat ttgggatatg attaggaaat cggccatctg gttccaaaaa
    15061 ttgggaacct gttgaatccg cacctaagct attcaatact ttttcagcaa agaatccacc
    15121 agcaccattt ccggcatcaa caatgatttt ccaaccattt agtggtttgc tttctagtgt
    15181 tccaatcccc tttttaattt ttgtgactaa atcttgcgca taaggtgtca aaaggtctgc
    15241 tttttctacg cttccagttt gttcgctttt tattttttgc tgctgtgata ggataaaagc
    15301 aatatcttct ttttctgctc ctccttctga ggtaaagaat tttattccat tataataata
    15361 gggaagatgg ctagcagtca gcataaccgc agcatcgcac gagaactgtt caaattgagt
    15421 ggacataaac atagcaggcg tagtagctaa ttcgaaatcg atgacatgta tcccttgttc
    15481 tttcagactt tggatcaacg cctctttcag tgctggtccg cttatcctac tgtcatgacc
    15541 aatcccaatc gtccattttt tatttggatc ggttgatttc ttctttttct ttatccagtt
    15601 tgctagtccc cagccgatct tttttaccat gtctgtcgtc aagtttactt catatccttc
    15661 aactggtaat gcgattcctc gaatatccga tccattttgc agcgctttca tatctttcat
    15721 ataaaagtcc tcctcattta ttccctcatt ttttccagct tttctgcaaa tcgctgtgct
    15781 tcttcttctg agcggcaaat aataatgaat gtatctacac cggccatcgt tccgacaact
    15841 tcagggtaag ccacttcatc tagccaattc gttaccacat cggcaccatt tcgttcagtc
    15901 aatacgataa ccatgaactg aacagactgg atacgcaaaa cctcgcgtct aattgctgta
    15961 cgcaaacgtt cttcatttaa agaaacagat ggctggttaa ataatgtata gcgtactatt
    16021 ttgttttcat tttgactttt tactaacttc atttctcgaa tgtctcgaga aatcgttgct
    16081 tgagtcacat cggcgcctgc ttcatgcagc cgcgcaagca gctcttcctg tgtcccaata
    16141 ggttgttctg taatgatctg gcggatcagt gcttgtcgct gtttcttttt catattagac
    16201 acctctcaat gttgattata gcaaaagata agtaatattt cttcatattt tggtattttt
    16261 tacttttatt ttttgttttc attcaggttg ctaacctttt tctattttaa cacgcaaaat
    16321 cgtcttcatt ttttcaggtg ctgattttct tggatcgctg atgtaaatct ctttgtgttc
    16381 tttagaagct ctttcatagc cttcttttgc gatgaatgct tctaatttct caaacgttgc
    16441 tggttcttcg gcatagggcc cgacgtgtaa gatttgggca caaagacctt cttccatctt
    16501 tttgaatact acttgttctt tcaagtcttc cggaagtttg ctagaacgct gaatggcttc
    16561 ttgtgctatc gcttctgtaa caaagtcagg ctgtcggatc atcaattgat aagaaaaatg
    16621 ttcttttttc atcactggtt catgcaaata cttttcttgc agtccccatt gtccttccag
    16681 tggatagaca gtatacatct gataatcagg aatcagccaa ttttttttag gcgacatgcg
    16741 tattgcatag ctgactgcat atagacaggc tactcttcgt ttaaagtctt cctgattcgg
    16801 gtcgccgatc cctctgatgc taaagaatga ttttttagga acctctagta tttgcgggct
    16861 ttttttcata ctatagctct tgtctttacg ccattcaatt ttttccattt ttcttcctcc
    16921 gcgaaattcc atttagttat ttattatcat acctcttttt gagtgacttg gctaatcgtg
    16981 ttgacactat ttttcagttt gtgctagaat aagtgaagta aataaatcgt acagataaca
    17041 cgtttcggcg tgtcgagaga gaggcggttt ctatttttat aatagaaatc actctctttt
    17101 tttacgaaat tttttagagg tgatgtaaat gacaaaacaa aaacagctgc ttttagtacc
    17161 tgcagtaatt gcaacaggga tgatgtcttt tgcgggcgta ttgatcgaga cggccatgaa
    17221 tgtaactttc ccaactctta tcagacagtt tgatttaacg actgcacaag tccaatgggt
    17281 aacaacgatt tatcttttga tgatttcgat cattgttccg ctgtctactt atttgaatcg
    17341 caattttagt ctcaaaaaac tctttttagc ttcaaatctg cttttcttgg caggtataat
    17401 gatcgatttc ttctctccta ccttcatggt cctattgttt ggtcgattgc tccaaggtgc
    17461 agcgacaggt atcgctcttc cgttgatgtt ccatattatc ttgacatttg cccccctcaa
    17521 tcgacgaggc gcgatgatgg gtgtcggtac tttgacaacg gccattgctc cagcaattgg
    17581 ccccacttat ggaggcatta tgacttcaca ttttacatgg aatcatatct tcctgtttct
    17641 tgtgcccgtt ttgtttcttt ctttgattat cggctggttt gctattcccg agatggcagt
    17701 acaaaaaagt ggttatttgg attggataag cgttttagga atttgtcttt tgtttagtgg
    17761 aagtctaaca tttttgagta cgttgggaac agtttccagc tggattgctt taatcatcgg
    17821 cggtattggc tggttccttt tttactatcg aagtaaaaaa gtggagcatc cactgatccg
    17881 actgtctatt ctttcaaata aaacattccg cttgtttttg attggcttct tagtttatca
    17941 gtttttatta cttggtgtat cttttgtctt gccgaatttc cttcagatcg ttcaaggaag
    18001 tagtcctttt gtcgctggat tagcgatgct tcccggcgca gcggttggtg ctttgctttc
    18061 tcctttttcc gggaaaatgc tggatcaata tggtccgaag aagccaattt ttgctgggct
    18121 gattctttct ttgattggtt ggctagcatt attcctactc ataggacacg cttcagtggg
    18181 tgtattgatt gcttgtcatg tgttttatat gatcggtatc ggattatctt acagcaatat
    18241 gatgaccacc ggaatgaatg cactgaatga agaactacaa ggagacggga acgcaatgtt
    18301 caataccttg cagcagtttt ctggcgctgt ggctacttca ttagtagcgg tcattatcaa
    18361 ttacgtccag catcatactt cccataacta tgaagtgtct actacattag gatcaaaagc
    18421 agcattaggc gtcttgttgc tgttgcttct cgtaagcttt gctcgattcg cctattatct
    18481 gttctttgct aaaaaagcgt agattccata tacggcaggt gttgcatctg ccgttttttt
    18541 gtgtgaaaat ggttgtttgt aaaaggaggc ttttttatgg aaaaaattgc cattatcggt
    18601 ggcgggatca ttggtatgac cctagccagt tacttagata catcaaaatt caatgttgct
    18661 ttatttgatg aagggacagg acaggcaact aaagctagcg ctgggattat ctccccttgg
    18721 ttgtcgaaac gcagaaacaa acaatggtat cgtctggcta aagacggcgc tgcttttttc
    18781 cctaaactta taaaagattt ccaacttgat caaacgattt acgcacaatc agggacactt
    18841 ttgcttcgac ctgaaaaaga tttagctgaa ttggctgttc ttgccgagaa acgaaaagaa
    18901 gaagcgccag aaattggaaa catcgaatgg ctttcacctg aacagacatc tcaagcactt
    18961 ccgttattga aaccagcagt ttcactgcgt atcagtggtg gtggtcgtct ggatggaaaa
    19021 gcatatttag aacatcttgg tcagcgttta gcacaaaaac aaatggagca gatccaggca
    19081 aaagtcactt tgcataagaa gagcgattct tggctcatca agggaccgtt tggagaatac
    19141 acagctgatc gtgtaatttt gacacctggc ccggcactta agcctttatt acaagaatta
    19201 ggctatgctg cagatatacg tccgcaaaaa gggcagctct tagcatttca gacatcttat
    19261 aagatgagtc aagactggcc tgttgctatg cttgacggag aagctgattt cattcctttc
    19321 atggatggaa agatcctttt aggtgctacc catgaaaacg atcaaggctg ggatttggct
    19381 cctactagcg aagcttataa gggattgact aagagtacag aacagttctt gactgatcca
    19441 gatcagataa aaaactggcc atacgagttc cgtgtaggta cccgtgctta tacgtcagat
    19501 ttttccccat ttttcggcgt actgccagat gatccaagct tactggctgc tagcggtcta
    19561 ggctcttctg gcttgaccac aggtccttat atcggctatc tattagccaa cttcttaaat
    19621 actggtcatt ggcctacgga agattatcaa aaggacatct ccacctatat tacgaaaaat
    19681 ccatcataga aaaacagctt tttcaagtaa ggatcccgag cgatattgcg catggattac
    19741 ttcttgaaaa agctgttatt ttagttcaat ttctgaatca aattctccaa actttctttt
    19801 gcttcatctg gcaagagatc ttttcctatc tgagcttcaa tgaatcgtaa acgaatatca
    19861 agtcgttgtt tcaccagatt tttataggac gtatcctcaa aagaaacagg aaaatctggt
    19921 ataggtaaat agtggatgtc ttcaaatgga ccaaacgact tgaattctgc cgtttgttgc
    19981 acgattcttt caacgattga caatgtcgtt tcaggtttga tttttttatc atcgaaatat
    20041 ccagtattca ggacatagca ggctgtttgc tgtttcaaaa acaactctcg aaaagcttga
    20101 tagtcttccc taagaggata gacacggaaa ggattagcaa atggttcaat gactagctca
    20161 tctagctgtc tttttgtagt gatattttcc gcgtctgatc gttttgttgc taatgttgca
    20221 ccaaaagacg ctgctaaaac tgggtcattg atcttgataa ttggcggaag cgaatcatct
    20281 ttcatgatcc aaaaaacagc atcgatcttt tcgctaagat gatcgactcg gtcgggtgtc
    20341 gcataacgtg atttgactgt ccgcccattt ccgtttcgta tatctcctgt tactaggact
    20401 ttttctcctt tacttgtaca tgtcacgccc acattctgac aagtaaggaa agaagaaatg
    20461 gccggatcat cagacggata gtcttgtgtt ttatcaaagt aagaaggttc caaggcagta
    20521 gcaaaaccat tttttcgaga aataacgaaa gcatcgtcat gcaagatcga aacagagtga
    20581 ccttccgtat gttttgctaa ggtaatcgtt gattttccag aaccggacaa cccaaataca
    20641 gccatcgtat aggtctggtc attactagca tattgtttca tcccaccgtg gcaggcaata
    20701 aagccattgc gatttcccaa tgcccaagca agcgtcaaag tcgctttttt taattctcca
    20761 aagtaacgta atcctagcac agccgctgta ttatggagag catcaaacaa aatgagtcca
    20821 tttggaaatg tttcatgtgt ccattcggga tcacaaaaga tataaagatc tccctcatca
    20881 aaggaaacag acttttgata agtctctaag atcttcgaat tcgctggctg aaaattcagc
    20941 aaataagaat acaaattgtt ctcaaatcct tctggaatca gcaaatgact ttgcacagaa
    21001 aattcttcag ataggccaac gattactttt gtatggtaaa actggcgctt gcgactttga
    21061 tacacagctt ctcttaaaat tttctcgtat ttgctttgtt ccttgtctaa gtcctcaaag
    21121 atccgccgag cacttgctgt tcttcccaca atgcttccat cattgaatac tagtgtccta
    21181 acatcagctg gtaaatctag ttcttcagca tgtgagattg ttaaatctgt gatgatcgtt
    21241 cctggactcc cttttgctag ttgataagct gtcttcctat caaaaacttc atgaacatta
    21301 ttaccgtaaa atgtcgtttc tacaatcgat cgaatactgg ataacagtgg atttccactt
    21361 gtgatttcgt ttaattcgag actctctttt gtactcatgg ctcctcctca ttccctaaac
    21421 aaaaaaacgt tttctttatt atacattatt cgagttttta tttgtactgc tgcgctgtgg
    21481 gaagcaaacg agttattttt cacacaaaaa aaccgagaac ttgaataaac aagtttctcg
    21541 gtttcatcat tttcctttta tcggcttagt accagccgtt tgctaaccag aatgttttag
    21601 cagcatccca tgaaccataa cgagatgtta catattgttc tgctacacgt tcttgattgg
    21661 cagctgaata gtcgccgttt aagtatgaag catctaattg ataacggccg atgtaacgtc
    21721 cgttagtagc tgtgtatgaa ccgcctgatt ctttttgtgc gatccattct tttgcagagc
    21781 ttgaagttgg cgctgttgca gcagctgggg cagcaggagc tacttcaact gtttcaacag
    21841 gtgcaacgta tgtatgttct tgcgttgctg tttcagttgt agatgctgta ttttctgttg
    21901 ttttgttttc tgttgttgct gaagaatcat ttgaagttgg gattgttaat tgttcaccaa
    21961 cataaatgcg gttgatgttt gcgatcgaat ttttttcagc aattgcatcg atcattgagt
    22021 tgtctcctgc aaatttttgt gagatcttag ataatgaatc acctgatttt actgtataaa
    22081 cttcatcagc gtgagcattt gtagttccta caaacattgc agtagcacca gcagcaaaag
    22141 ttgtacctaa aacaattttc ttaaatgaat tcatattaaa ctcctttttt tctccgtttt
    22201 ttattctttc gacgctatta actttatcat ttagaaatat ttcataggta aaagaaacat
    22261 gacatttaat gacaaaataa cgaattattt aatatttgta acatttaaac aaatattttt
    22321 ttgatacaaa accataaaag ccttgttgta taagca
//
LOCUS       DAHUVV010000037        22281 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.037, whole genome shotgun sequence.
ACCESSION   DAHUVV010000037 DAHUVV010000000
VERSION     DAHUVV010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.037"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..407
                     /locus_tag="NK767_001437"
     CDS             <1..407
                     /locus_tag="NK767_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCH6694438.1"
                     /translation="LREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITDMPK
                     APKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRISRE
                     VGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            1027..1191
                     /locus_tag="NK767_001438"
     CDS             1027..1191
                     /locus_tag="NK767_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694439.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            1188..1418
                     /locus_tag="NK767_001439"
     CDS             1188..1418
                     /locus_tag="NK767_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694440.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            1603..2148
                     /locus_tag="NK767_001440"
     CDS             1603..2148
                     /locus_tag="NK767_001440"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6694441.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            2149..2466
                     /locus_tag="NK767_001441"
     CDS             2149..2466
                     /locus_tag="NK767_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCH6694442.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(2623..2787)
                     /locus_tag="NK767_001442"
     CDS             complement(2623..2787)
                     /locus_tag="NK767_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HCH6694443.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            2889..3410
                     /locus_tag="NK767_001443"
     CDS             2889..3410
                     /locus_tag="NK767_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCH6694444.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            3397..3870
                     /locus_tag="NK767_001444"
     CDS             3397..3870
                     /locus_tag="NK767_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCH6694445.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(3877..4035)
                     /locus_tag="NK767_001445"
     CDS             complement(3877..4035)
                     /locus_tag="NK767_001445"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HCH6694446.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            4128..4889
                     /locus_tag="NK767_001446"
     CDS             4128..4889
                     /locus_tag="NK767_001446"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCH6694447.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            5109..6428
                     /locus_tag="NK767_001447"
     CDS             5109..6428
                     /locus_tag="NK767_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCH6694448.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            6444..7664
                     /locus_tag="NK767_001448"
     CDS             6444..7664
                     /locus_tag="NK767_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCH6694449.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            7699..8376
                     /locus_tag="NK767_001449"
     CDS             7699..8376
                     /locus_tag="NK767_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCH6694450.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            8373..9566
                     /locus_tag="NK767_001450"
     CDS             8373..9566
                     /locus_tag="NK767_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCH6694451.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            9581..9907
                     /locus_tag="NK767_001451"
     CDS             9581..9907
                     /locus_tag="NK767_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCH6694452.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            9911..10246
                     /locus_tag="NK767_001452"
     CDS             9911..10246
                     /locus_tag="NK767_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCH6694453.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            10243..10638
                     /locus_tag="NK767_001453"
     CDS             10243..10638
                     /locus_tag="NK767_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694454.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            10635..11006
                     /locus_tag="NK767_001454"
     CDS             10635..11006
                     /locus_tag="NK767_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694455.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            11022..11582
                     /locus_tag="NK767_001455"
     CDS             11022..11582
                     /locus_tag="NK767_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCH6694456.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            11707..12018
                     /locus_tag="NK767_001456"
     CDS             11707..12018
                     /locus_tag="NK767_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694457.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            12042..12233
                     /locus_tag="NK767_001457"
     CDS             12042..12233
                     /locus_tag="NK767_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694458.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            12233..16089
                     /locus_tag="NK767_001458"
                     /pseudo
     CDS             12233..16089
                     /locus_tag="NK767_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            16090..16962
                     /locus_tag="NK767_001459"
     CDS             16090..16962
                     /locus_tag="NK767_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HCH6694459.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            16959..18008
                     /locus_tag="NK767_001460"
     CDS             16959..18008
                     /locus_tag="NK767_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694460.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            18012..18836
                     /locus_tag="NK767_001461"
     CDS             18012..18836
                     /locus_tag="NK767_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694461.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            18854..20914
                     /locus_tag="NK767_001462"
     CDS             18854..20914
                     /locus_tag="NK767_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HCH6694462.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            20931..21080
                     /locus_tag="NK767_001463"
     CDS             20931..21080
                     /locus_tag="NK767_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694463.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            21077..21523
                     /locus_tag="NK767_001464"
     CDS             21077..21523
                     /locus_tag="NK767_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694464.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            21525..21662
                     /locus_tag="NK767_001465"
     CDS             21525..21662
                     /locus_tag="NK767_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCH6694465.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            21697..21939
                     /locus_tag="NK767_001466"
     CDS             21697..21939
                     /locus_tag="NK767_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HCH6694466.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            21955..22152
                     /locus_tag="NK767_001467"
     CDS             21955..22152
                     /locus_tag="NK767_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCH6694467.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            22163..>22281
                     /locus_tag="NK767_001468"
     CDS             22163..>22281
                     /locus_tag="NK767_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCH6694468.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSS"
BASE COUNT         7702 a         3776 c         4982 g         5821 t
ORIGIN      
        1 tgttacgaga ggtagatttc aaacagacaa gatgtaatgc gagagatgtg ctgaagaact
       61 ttcggcgttt ggagcggatg gcaggtcgct ctttgataga tattaagtcg ccgattatta
      121 cggatatgcc gaaggcgccg aagcacggca ataaggcaga agacgcaatc attcagatga
      181 tggatataga agcggagaga gacgcgattc tagcagcctt gatggctctt agtctgatta
      241 gtcgtcagat actctactat agcttttgtg tgccagatag cttctcaaac tacagaatta
      301 gccgtgaagt gggttattca gaaagaagta tacaacggat gaagtcggaa gctctaatag
      361 agtttgcaga agcatataaa cacggaagaa taattgctta taaataattt ggcggctttt
      421 tggcggaatg atggcggttt ttagctattt accagtgata ttatggtagt gtcgaaagat
      481 tagtgatagg tctaagacaa aataataata aaaggaacat cgttttatta ttgtttcaca
      541 attaagcttc gatagacagc aacggaaata ttaagaataa ggatgtgaat tttaactcct
      601 tctaaattgt tcttattatc tatcatccgt tgctgtctat tgttttttta attattcaca
      661 cgataactaa aggtgagcga agagaaatga ttccattaat aatttcaatt tttgcgcttt
      721 gtcttaatgt ctatatgatt ggatttaaaa atgggcaaaa taaaagatag tagtaactaa
      781 gaaagaaatt tttatattgt cactgtggcg gaaagggtag acgctaagca tgtgtgctag
      841 gtcaatgctt cggcaactat gcaatgttcg attcattgcc agtgactttg gcaaccgagg
      901 catcggcggt ttaaaaatat aggggtgcgc aatttcgtac gcgttttgtg catcgtgtag
      961 gttgctatta catattagat cactcgttga gtggtctttt tattttacat aaaggaggta
     1021 acaacgatgt atagaccaca atacttagaa cagaagtatg aagtaatcac tgtgcaaaac
     1081 ggtaacggtg agatagtacg aaagtataga agaccaataa agagcgatac atataaacga
     1141 aaggaaaaca atgaagttat ttcttttcgc agaaggagaa aagccaaatg agaaactact
     1201 ggtatgtatc gctaactaat gaatatcctc gaaccattga tgattgttca gtgcgtgttg
     1261 tgcgttctgt acaaatcaaa gggaagtact ctattgtcga aatgctaaga gaagctacac
     1321 caaacgaagt ggataaatgc aagctgatat attgcggtca tggctattgg aaagacgagt
     1381 atatccaaca aaacattgag aggtggatag atagatgagt tacctcgaac atttgaaacg
     1441 ttgctacatg cattctaaga ataaacttcc tgacagctac actgtagatg atgttgctat
     1501 acatgtattg aagactgaga gccatagcag tcctgatggt agcagtaagg agcagacgct
     1561 cgcgtggttc aagttcttta agtggattaa ggaggaagag taatgcacat gcacatagga
     1621 gaaaagatat ataaattgag acggcttaat aacgaaacaa tgaacgactt agttaaacaa
     1681 actggagtgt caaaatcatg gttatctgat attgagtctg gaaaaaagca acgcgttgat
     1741 tttaataaga tatgtgcaat agccaaacat tataaagtta gcttagaata ctttcgggat
     1801 gattatttgg atagtgatga acacgaggat attattcctt tgacaggcga aacaagagtt
     1861 aaatcagtga aatcgcttga acaaagccta aagtatctga ataacaaaca agaacctaac
     1921 cttctagaga tcaaactaaa agatactgac tcagtaccag aggtttggta caagggtgag
     1981 aggttggatg aattgcctaa aggattagta gatgtctcgt atcattggaa gactgatgat
     2041 tttactaatg atgatagagg agcgaacgac atcacgattc aatacttttc tagctttaat
     2101 gataagtatc cagataagaa aacaatcgga cacaagagag atatgtaaat gaaagaagtt
     2161 agacctagag acgaaataga caaactatac aagaccaaac gatggcgaga cctaaggcaa
     2221 gtagtaatag ctagggactt cggcatgtgc caagagtgca agcgtcgagg gcggaacaca
     2281 aggggcacga tcatccatca catagtcgag gcgagggaag acctgtcact gttctggtcc
     2341 gtagataacc ttgaatgtat ctgtgtagct tgtcacaaca gagagcatcc agagagatca
     2401 ggcgggaaga agaaaccaaa acctaaatca catatcgtta aaatgtattc aactcctgaa
     2461 agataagttt gcagcgaaat gaaggtagcc cccctactct aaaagattaa agagtaaggc
     2521 ttgagaagaa cggtgctgtc cttccttcgt aaaaagaccg cttttcaagt tttttggaga
     2581 aaaaggaaaa agccgaccaa tttaagccgg ctttggacga agctatttct tagtccattt
     2641 gtttcctttt tgagaagtag ggggtaatcg gtcgcctgga tcaattttta cttcttgtcc
     2701 gccttggaca tttccaccac gaggacccac ttctttgtag gttccttttg gtttgttgtc
     2761 ttcgccgggt ttatagagtt ctcccatagg aatccctcct taacaaattt cggcacagca
     2821 ctgccgataa cctaattata aggattgtga taacgatttc aatctatctt ttgaaagaag
     2881 gtgatattat gccgcaacca gcgaagagtg caaaattaca attattaaac ggaaacccaa
     2941 ataagaagaa taccgaagaa ctccgcaagc gagcggccgc agaagacaaa ttaaaaatgg
     3001 ctactgacaa aatcaaaccg ccgttatggc tagattcgct aggaaaggat acctttgagt
     3061 ttatcgccga tgaattgctg tctgtggatt taatcagtaa tccggacgtc catacaatgg
     3121 ctctatactc caattggtat tcgcaatacg tttctttaga aaaacagctt cgaaaactac
     3181 aacgagagta caagttgaac tatgcgcttg cgaaaaagga ggcagaggcg agaggtgagc
     3241 cgtttaatga acctaatgaa ttaattggta atccgctctc tcggcagatg gatacagcgt
     3301 ctcggaatct ccgttctttt ggcgctgatt taggactatc accagcagcc agagctaagt
     3361 tagctattaa gatggctgat gatggtggtg atgacgatga cgacttctaa tattttggat
     3421 atgtcttaca cagaacgtgt ggactattgg caaagctatc ttgaggagca agcttcttgg
     3481 ggtggctttt taaaatgtcc atatccggaa ttgttaacta cttggtatgc ggaacgatta
     3541 atcgatggaa gcataccagc cagcaaagaa aatattcaag ctgctaaacg gcatatgcgt
     3601 gatttgcagc gccaaggaac agatgatttt ccttggatct ttgacgaaga aaaaggtcac
     3661 cggcctatta gatatatcga aaaaaaatgt aaaccaactg aaggcgactt tggttcgttt
     3721 gttttgcaac cttggcagca tttcataatt ggatccatgt acggatgggt acatcgtgat
     3781 acaggagagc gtcgcttccg cgaggctctt atttttgttg gacgtaaaaa cggtaagttt
     3841 tgccgtttaa aaatcgggca aaatcggtga aaacctttat ttttggcttt cttttagtaa
     3901 taattcgata gctttatcaa gcaatttaga cataggaacc atagtttctt ccgacatttt
     3961 ttttaatcct tcgtataaat ctttatcaat tgcgttagaa attctaattc tgttttttag
     4021 tccgtatttg tccatgtgtg acactccttt ttattttact ataacatccg attccaccgc
     4081 ttgcaactac catcaattga tggtagtata taaaagaggt gagtagattg aaaaaataca
     4141 ttatttacaa acacttaaat aaaataaatg gcaaaattta tatcggtgtc acaaatgata
     4201 ttggcagaag gtggaggagc ggtgggattg aatataaacc accaaagaac gaaacccaac
     4261 accacagaag tttttggaat gcaatacaaa aatacgggtg ggaaaatttt gatcatttga
     4321 ttattgaaga agacttaacg atgaaagaag cttttgagaa agaaaagttt tatattgaac
     4381 tttatgactc aactaataag aaaaaaggtt ataacatcgc aaaaggcgga aatggcggaa
     4441 taatatataa agttcacccc aaaggaatgc tagggaaaaa acaatctaaa gaattttctt
     4501 caaatcacag taaatgggca aagaaccata agaataattg tatgacaaac ggtgatgttg
     4561 tatggggtgt tactcacgaa cacccaaaag gcatgttggg aaagcaccac agcaaagaat
     4621 caattatgaa aaagaaagca tatagtggtg aaaatgctgt aacctcaaaa ccaattgtcg
     4681 caattgaacg ggatggaact aaaagagaat ttcacagcgc taagctttgc atggcttact
     4741 attcgattag tacatgtgtt ttttaccgac ttttaaaaga tggcgcccct tatgtgataa
     4801 atcctaaagc gaactacaga aataaagaaa aaatattagc tatcgaagga attatgttca
     4861 aacataaaga agataccgag gtaagttagt agattgcgaa aggttactaa ctaccgtaac
     4921 gcgtaggagt tgaataaata taatgctccc aagagtgtcc gacagcagat aatttgctga
     4981 aaatgtacgc taaactgggc cagaactgac tgaccgatga aaatgagggt gacctccaga
     5041 gtgcgagata aaaagctcgc agataataac aatcgaaaac atcactaatt tcaggccttt
     5101 ccacatacat ggtcgcttat gatgatgaac aaggcgccaa cgtttacgta ttggcaaatg
     5161 ctcgtgatca agcaagcttg ttgtttgata aggccgcaga aatggtcaaa caatcgccgg
     5221 cgctctttaa gaaatttggt aagcctaaac gatcaagtat taattatgct cccgcctttt
     5281 ctaaaatgga accacgcgcc tcagatagcc ggaaattgga tgggctaaac actcactttg
     5341 gtatttttga cgagatccac gagtttacga attacaatct gatcaacgtt atcaagaaat
     5401 caagaggaac cagaaaacag cctctgatag tttatatcac aactgctgga tatgtattag
     5461 atggtccgtt gatgtcttat tttgagcaag gtgtggactg tttggaacat ttggaagatg
     5521 acatcgatga acggactttc tattatctgg caaaacttga cagtgcggaa gaggctgatg
     5581 acccaagatt atggatcaaa gccaatccga atatttgtct aatgaatttt gttggcatgc
     5641 tagatgacta tgttaaggat aaaaaagatc caaaagaata tgctgactgg attaccaagc
     5701 aatttaactt gttttccgat atcgatgagc tgtcatttgt cgatatgcct accattaaac
     5761 gaaacaataa aaccatcgat attgaaacgc tcaaaggtaa gaagtgtgtc ggtggttttg
     5821 acttgtccga aacggaagac tttaccgcag ccgttttaga atttccgctt gaaacaggcg
     5881 aggtattcat tttgcaacac acatggatcc cacaagctag atttgatcga gataacaatc
     5941 aagagcgtat caaagcgtgg gagaaggtgg gagatctaac gattattcct ggtgattacg
     6001 tcaattatga atacgtctta aattggtttg tagaaaattc gaaaatctat gacattgtaa
     6061 aaatcaatta tgacaaggcc aaggcgctac gattaaataa agaactagaa aatgcaggat
     6121 ttgaaaccaa cgagattcgg caagggtttc tatcattagg tgggccaatg caaaacttca
     6181 aggaaatgct attggacggt aaggtgattt tcaacaattc caagctttac cgatggtatc
     6241 tatccaacgt caagctggtg atggatcgca actcaaactg gatgccgtct aagcagtcca
     6301 agagtagaaa aatagatggt tttgcagcaa gtttgaacag ccacgccgaa gtgttgaata
     6361 tgttggttaa tcctgtcgga accgggaaag taacctatta ctcgatttcc gatttaatga
     6421 atatgtaaga aaggtgtgga ggaatgagta ttttagatcg tttgcgttct tttggccgag
     6481 cgaagccgaa agcgagcaaa caagagtatt ttttgaatga cccgggattg ataccgtatt
     6541 tagtcggaaa agatgaaata tcagaaggga ttttttccgt aattagccgt gtatcgaacg
     6601 tttttgcgtc tctccctctc aaaatgatag atgtggagtt tggccaaccg gacgactgtc
     6661 ctgcatacaa cttgttgagc gaaggccctc gatattttac aaagtttgat tttttccggg
     6721 acgtggaagt tttgagaaac taccaaggga atgcgtatgt gcagattttc cgaaatatca
     6781 atggagaagt agcagatatg gcgttagtaa aacctggtgc ttgccatcca gtgattgata
     6841 tggatagcgg ggagctttac taccaagtaa ctgcgactga caaaggcagt tacaagcaag
     6901 ttatctatgt acattacatg gaaatgcttc actttaaaca accgaggttt ggcggcttgg
     6961 aaggtacaga ccccacaaaa gtattaacga ataccctcgg atatgatcga gaagtccgaa
     7021 aaatctcttt aagtcagctt aaaggaagta atgaagggct aaaagttaag tttgctagca
     7081 atatggatga agaagctaaa aaagctacag ttaaaaacat tgctgatttt tatcgacaaa
     7141 acggtggact acttgtggaa gaaaacggtg tagaaatcga acgtttacaa cgagagctgg
     7201 tagacagcaa gcttttagat actgataaag tatctcgctc cagaatcgct atggtctaca
     7261 atgtgccgga gcatttcatc gggaataacc agtcgagtta ctcctctcag gaacagctca
     7321 atatggaatt tttgacgtac aatctagtac cgaccgttaa tcaatatgaa gcggaactaa
     7381 ataagaaaac actatcgaga accgaaaaag caaaaggtta tcgatataag tttaatgtcg
     7441 caagtttgct aagagctgac actcaggcaa ggggacaatt ctatcaaatc atgcgacgag
     7501 gcggagcata ctcagcgaat gatgtccgaa gatttgagga cttgcaaccg ataaataaaa
     7561 ccggtatgga tgattaccat atttccggag acctatatcc aatcgatatg gatccaacat
     7621 taagaaaaac aacctcgtct aaaagcgtag ccgaaaacgg ttaggttttt ttagtttgca
     7681 ccgaagggag gtggaaaaat gaaaaaagtg acgttaagcg gcgatgtcgt ggataacgat
     7741 accgcgtggc tttatgactg gtttgggatc aattgtatct caccagggaa aatttctgcc
     7801 gctcttacag aagcagcggg ggatgaagta gaacttgata tctcatcgaa cggtggggat
     7861 gtcctagcgg caagcgaaat atataccgct atccgtgcct atccgggaaa ggtatccgga
     7921 aatgttgtga gtattgcagc aagcgctgca agtgtaatcg cttgcgcttg cgaaccgctt
     7981 agaatctcac ctacggcaca catcatgatt cataacgcat gggtgaccac tagtggcaac
     8041 gctgaggaat taaaagccaa tgcagaaatg ttaagcagtg tggatgagtc tattgttaat
     8101 gcttacgaga tcaaaacagg actcgatcgg aaaaaacttg ctgatttaat ggcgaaagat
     8161 acttggttaa atgctcaaac agcagtagca gaagggtttg cagatgaaat tatgtttgca
     8221 gaagcaccag taacggtact caatgcctct caaccggtta ttccaaaaaa cgcagtaact
     8281 aagttgaaaa atttaatact caaagcggaa gcaccgcaag aagaaacact cttacagaaa
     8341 aaactaaaag ccttaaatgg agggaaaaac gaatgaattt agaacaatta aaaaatgcgt
     8401 gggtcgaggc gggaagtaaa gtctctgact taaatgcaca actcaatgca gcattggttg
     8461 acgatgaaaa aacagaagaa gatgtagtaa gtttgcaagc acaagtaaaa gcagcacggg
     8521 ctaaacggga cggattgaaa gagcaagtgg caaatatgga agccgaacaa gtcttaaacg
     8581 tcaaaaaaga accattagat aaaaaagatg aaaacttgaa aaacaagttt atcaaagact
     8641 ttaaagcgat ggtcaatggt gatcctgcta ttatggctac tttgacatct aatacggatg
     8701 aatctggtaa tgctatcgga ttgactattc ctgtagatgt gcaaacgact attcatactt
     8761 tggttcgtcg gtttgactct ttgcaagaat acgtaaacgt tgaaaaagtg accactacca
     8821 gcggttctcg cgtttatgaa aaatggtctg atattacacc gttgactgct ttggatactg
     8881 aagacggtga aatcccagca aatgatgatc ctgcacttta cttgatcaaa tacttgatca
     8941 aacgctatgc aggtatttcc acagtaacta acagcttgct aaaagatact gccgaaaaca
     9001 ttttggcatg gttgtctaaa tggatcgcga aaaaagtagt tgttactcgc aatacaaaaa
     9061 tcttggcagc tattgatgga atcaaagcgg cacaaaagaa agatgttaca gatgttgatg
     9121 gaattaaaga tatcgtaaac gtccaacttg atccagctat cgaagctaca tctatgttta
     9181 ttacaaacca agatggcttc aatgttttag ataaagtgaa acgtgctgat ggatcttact
     9241 tgttacaaaa agacgtagct tctgcaactg gatatacctt cttaggtaaa ccaatcaaga
     9301 aaattgcttc tcgtttcttg ccaaacaaag gtacgcaagc tactcctaaa tatccactgt
     9361 acattggtga tctgaaagaa gccgttacat tgtatgatcg cgaaaacatg agcttgctga
     9421 caacgaatat tggtggtgga gcttttgaaa cagacaccac taaagtacgc gtcatcgatc
     9481 gcttcgatgt gcaactagtt gatgatgaag cggttgtttt ggctactttt acaactattg
     9541 cgaacgagac accggcgaaa gtttaaggag ctgattcctt atgattcttg atcctaaaat
     9601 ggatttaggc gaaatcaaaa acgcactaaa gattgatacc gatgatgacg atgtggaagt
     9661 aagccgtgcg gcacaagctg caattgcata cattagaggg gctatcggga atgataagcc
     9721 ctctttttat aagcaagaaa atgaaacggt tgatctgatt aatttagcta tcctgcaatt
     9781 agcagatcat tattacaacg ctcgatcagc aaccgtgagt gggaacttgc gagagtacga
     9841 tttaggtttt acaagcctaa tcttgcaact caaagcaagt tatttgcttt ttgtggagga
     9901 ggagtagcgt atgcccctta tccaaacagg aaatttaaat caacgcatca agtttgtccg
     9961 agatacgacg gttaaggatg aggacgggca agttgtcccg acttctacaa ccattcttac
    10021 ttgctgggca agtgtgcaga cacaacgcct gaacgatatt aagacgtcga ttggtacggc
    10081 tttggaagga acactgacgt tcattatccg ctaccaacaa aaatcagagc tgaccaatga
    10141 tatgaaagtg cgttggaatg gaaaaacgtt tgaaatcatt acgattacga aaggcgagtt
    10201 tgcgaaggac ttcacgacaa tcattgcaaa agaggtttca aaatgagtgt agaagtcgat
    10261 gcaaccgaag tgtacaaagc gcttagggaa gtaaaagcaa acgttcaacg agtggaaagc
    10321 ccagcactta gaaaagctgg ggagtacgct caagaagagt tacgacaaaa cacaccttac
    10381 tgggatggaa cgaagtcaaa cggtaaacgt ggttcgtata tgcaagaaca tgctaagaac
    10441 catgtggtta caagctcgat aaaaaacgga ttggtagaag tcggctatga caaagatgtt
    10501 tcttggcgga tgcactttat cgagttcgga acaatcaaac aacgtccaaa aggtttcgta
    10561 caaaaaacac aaaagcaaat cgaaaaacaa gtaacacaaa tcattgctga cgaagtaaaa
    10621 aggaggctag gactttgaaa acggcagtat cacaagtcta ttcaattctg aatagcaatg
    10681 aaaaaacaaa gaacattgat ttttacacca atagtgttcc ggaatcagct caaacagtac
    10741 ctagccttcc agttggcaga attacagaga tatccggcaa ctatgaagat tttgcaagca
    10801 acaatccttt gaccattcaa tttaacgtac aggtagatgt atgggtgtca accatgaaag
    10861 aggttgatgc cttttatttt gcccttgatg aggttatgag ggggaatggt tggcaatgcg
    10921 catacacgga acaaacagat gacgaggact tggaaggtgc aaagcggatt atcaaacgat
    10981 atgtagcaaa tatttcacta aattaaaagg agagaaaata gatggcaaca gtaggattcg
    11041 agagcgtcat ttttggcgta aaaacaggtg caggcggcac tctaaaagaa ttagtagcag
    11101 ataagtcgaa aggcggagcg atcgaagcta aaattactgg attaggcgca acttctaaca
    11161 caacatacgc ttcaaacgta ccgttcttca ttgcaagtaa aggggtttcg tcgccaaaag
    11221 ttacgcttga cgtggcagac ttaatggata acggcattta cagcgaaatc attggtgcta
    11281 aaaccgtgga tggtgcaaat gtaattggtt cagaaactga agcgccttac gtgtcggtag
    11341 tcatggttac agcgaacaaa gaaggaaaac gcttgttcat gggattgaca aaaggaaaat
    11401 tcagtcatcc agatatcgac atgaaaacag ctgaagacaa aggggtagaa ttgcaaaccg
    11461 attccatcga aggggaattc atttctgatg aacgtggcta tgtatactta acagccgtag
    11521 aatcagaagg aatgacctta caaaaattca aggacttggt aaataacaaa gagggggagt
    11581 agttaaccct gcatctacac caacgacaga tacagggaca ccaaaagaac cagaaccaaa
    11641 aattgataca caaggttagc catttttggc tagccttatt ttttgtaaaa acaaggagga
    11701 aaacaaatga ttgaattgca attgaaactt gacggaaaga aaaaaacatt caaacaacaa
    11761 gatatttccg cacgtgcaat gcgtgagtgt atcaaatttt acgagaaagc ggaaaaagca
    11821 gacctaactg atttagaagc gattgattca atgattgcaa ttacagcaga tattttccaa
    11881 gatccagcag ttacatttga tgctatttta gacggtttga ctgcgagcga gttagtaccg
    11941 gcattagaaa gtgtttttga acaaataaat gaactgggaa acaatgaaaa aaagcagacg
    12001 gcgagcaaaa agaaataagt ttttctgaag ctaggaaagc aatggatcaa atctacaaag
    12061 atttaatcga agcaggttgg acgatgagag atgtggacga agccgactat cattatttgt
    12121 tacacctttt tggagaagtg gagagtggcg aagaatatgt agatggtgct gatttcatca
    12181 aacaattttt atcggctgaa gacttagtaa aacttgagga aggaggtaaa taatggcagg
    12241 aaaaggacaa ccggcaggaa atatcaagct agggattagt ttagatagca ctagttttgg
    12301 taacacgctg gacgaaatca atgcgaaagt caaacaagct gagtcgaata tgcgtgccaa
    12361 tctaaaggct tatgattcag caggacgttc atacgaagca cttagtcaaa agacgaaaga
    12421 cttgtctacg gttatggaag ggcaaaacgc caaagtaaga gaattaacaa agcgccgtga
    12481 tgaagcgatt agcaagtatg gcgaggaatc gaaacaagtt gctaacctta acacacagat
    12541 aaacaatgct accgcaaaat ataatgctta cagtcgccag ttgaacgaca caaaaaaaga
    12601 attggtgtat tccaaaacag ccgtcaatga tttatctaat gaaatcaaag aaaatgaacg
    12661 acaaatgaac gccgaagtaa aagcgttgaa agccgctggt gatgaatctg gtgcttttga
    12721 agcaaaacaa aaagggctag ccaaacaaac ggaattatcc gagaaagcta tcgaagaaca
    12781 gcgcaaagtt gtgaaactga tggctgatga gtttggcgat tcagcaaatg aaaccgaaga
    12841 tgcaaaaagg gcattagaaa agttagaacg acaaagccaa atatctagca ggcaattaga
    12901 agcactcaaa agctccagcg atcaatcagg aaaagaaata gaagattttg gcgacaagtc
    12961 cacaaggtca gctaggaaac tggacggact aaaagacaaa ttaagctcgc taaaaagcgc
    13021 attttcgttt ggtgcagttg ctggattagc gcataacgct attagcagtg tagtaagtgg
    13081 cgtgcaaggt ttggttggcg aagcagtaaa cgcatcggat tcattgatga agttttccaa
    13141 aaccatggag tttgctaact ttgggaagtc acagatagaa agctcgaaaa aagaaatgaa
    13201 agactacgcc gataagacgg tttatggttt agaagaaatt ctgaacacaa ccgcacaatt
    13261 ggcatctaat gggattccta actatacaga actaaccaag gcggcaggta acttgaatgc
    13321 cgttgcaggc ggttctagtg atacattcaa atccgttgcc atgatgctaa cgcagacggc
    13381 aggagctggg aaactaacaa ctgaaaactg gaatcaatta gcagatgcga taccgggtgc
    13441 ttcaggattg ttacaagacg ctatgttgaa aaacggagct tatacaggaa acttccgtga
    13501 tgcaatggcg caaggtcaaa tcacttccga cgagttcaac caagcaattg tacagttagg
    13561 tatgaatgac ggagcagtta aggcagccac ttctacagac acattgagcg gttcttggga
    13621 gcagatgaaa tccactgtaa taaatgggct acaaagtatt atagaaaaaa taggcgttga
    13681 aaatatcact ggtttcatca acagagtaac aaaagggatt gaaaattcta ttcctaaaat
    13741 tactcaattt ataggttggt tgagagatat tggaacgtgg atcgttgaaa atagagagcc
    13801 actaacatgg attgtcggaa tcataggcgg aattacatta gcagtaaaag cattgaacgt
    13861 agcaagtatg ttgctggcaa ttactggcgg aacattggca gccccttttg tggcgattgg
    13921 tgtagcatta ggcgcactag caggtgcttt ggtgatagct tatacaaaat ctgaaacatt
    13981 tagaaatata gtcaatgcgg cttttacagc tgtgaaaaac gtagttatga gcgttatcaa
    14041 taatttggtg gaatactaca aaatgttgtg gagcgtgttg cagtggcttt gggaaaaaat
    14101 aaaagaatgg gcttcatgga ttggcaataa attcattgaa atgaagaaca gcgttgtgaa
    14161 tacggttcag aacctatgga atggcgtgaa aaacttcttc agcaatggcg ttggagatac
    14221 gtggaataag gtagtcggtt gggtaaaaaa cattttcaac aaagcaactg aattgaagaa
    14281 caacgtttct gatgtaatcg gtaacctgtg gaatggtatc aaagacacat tccgtagagg
    14341 tatcgatacg gtattcaatt ggttttcaga actaccaacg aagatgaaga atgccattat
    14401 tggcggtaaa aacgccattg ttgatgcgtt caaaagtatt ttcaacgcag cacttaaagc
    14461 gataggtaaa ccagttaacg caatcatcca tggagcttca tgggtactag aaaaattggg
    14521 cgcagaacct ctacaagaat gggatgtacc acaatacgct acaggtacac cagcaggtgg
    14581 gcacccaatc aatggtccaa tgatggttaa tgatggacgt ggagcagaaa cagttatcac
    14641 accagatggt agagccttca ttcctaaagg acgcaatgtt gttctaaacg caccaaaagg
    14701 aacgcatgtc ttgacagcag aagagaccgc gcaacttcaa ggttctaaag ctcctaagta
    14761 tcgttacaaa aaaggtacta acttctttgg taacatgtgg gatagtgtga aaaaggttgc
    14821 tggtaatgta ggtcacacaa ttaaaaacgt agtaggtgac gtgtgggact ttatttcaga
    14881 cccgggagcg ttagctagaa aagtacttgg ggggttagat gtattaggtg gattgacaaa
    14941 gtatccatta gaagtaggta aaggcatcct atctaaagca acaagtgcac tgactgaaaa
    15001 aatcactggg ttgttctcat ctggtaactt agatacctcc gtaggaacaa atggcgtcta
    15061 taaatatttg gctgatgttg ctaagtctgt gatgaagaaa ttcccaggat ttgtggcaac
    15121 tagtgggtat agacctatag accaggtgac ccttattcac atggtaaacg taatgccatt
    15181 gatattgcat taccaggcgt cacaggaggc tcacctagat acacagaagc agcaaactac
    15241 gcttttgaca aattcgcttc caaaattggt tacgtaatca ctaatgggaa agttcgtgac
    15301 cgttcaggac aatcaggtca accagcaact ggtgcatggg agccatggcc cgatggagat
    15361 cactatgatc atgtgcattt aaacggtgtg aaagacccac aaaacactca aatttcagga
    15421 gatagcgtgg gaggcagtgg ggtagaaaga tggcgcaatg tagcaattag agcgttgaaa
    15481 atgaccggtc aatacagtac tgcaaactta aatgcattac taaatcaaat gcgtacagag
    15541 tcaaatggta atcctaatgc agttaacaat tgggatatta acgccaaaaa tggaacacca
    15601 tcaaaagggt tgctccaagt gattgaccca acattcagac agtatgcaat gccaggattc
    15661 aacagcaata tttttgaccc actatctaac atcttagctt caatcagata cgcactatca
    15721 agatatggct cactaacaaa tgcctatcgt ggagttggtt acgcaaacgg tggaattgta
    15781 aaccaacatc aaattgcgga aatcgcagaa ggaaacaagc cagaaattat tattccgtta
    15841 gataaggcta aacgatcaag agcgatgcaa ttattgtcta tcgcaatgga caaactagga
    15901 gtttctccta aaacttatgg aaatgcgaca actgtttcca gtgattacga aatcctagaa
    15961 gccattgaaa aacaggcggc aacaacgaat cagctgttat cgttattgct ggcatttttc
    16021 aaaggaaata gccgaactga tagagattta gctttagata ttcagaagat cttggttagg
    16081 aggatgtaga tgcgaactgt attactgaga aatagaataa acgaagaaat tgatttgtcc
    16141 acagaagact attttgctac tgggttgagt aatatggggt ttgaagtaaa aaaagaacat
    16201 gtgggacagt gggggaattt cagagaaagc agtgagagcg ttgaaatatc tgaatttcag
    16261 tcatctgtaa tcatttctgt gcatgggttt cgagaaaaag aactgtacaa ttcacttgtg
    16321 cagttcctat cggaaggtcc gtttgaactg gaatttgctt ttgacggcga aacaatggta
    16381 agaagatgca gtctaaaatc tttaagtaag actgaaatcg atccgaaaac atcgttacta
    16441 acagacactt tagaactata tttcacatca aactggtatt ccgtaaaacg cgaaaaatta
    16501 attcagcgac cgaatgtagt gaaaacacgt ggtaaggttt ttccgtataa aagatcttat
    16561 atctacactc aaaacttgtg ggagaaaaaa ggtgtattca aattcaataa taattctgta
    16621 tacctaacga atagcaagga acggatgtcc ccgctaaaaa ttcgtgtgat tgggaagtgt
    16681 tcaaatccgt attgggaagt aatccaaaac tcacaaatca tcgcaacgga tggatacttc
    16741 atagatatga ctgaaacaca gactctagaa gtatcaagcc tttttgaaga tacaacggcg
    16801 attttaaaag atattgcagg ggtagaatct tctgtctacc aacaacagga ttatacaaaa
    16861 actaattttg ttcaggcccc aactggagaa ttcagcattg tgtttcatgt tgggggggca
    16921 gacgtagaga ttgagttata cgaggagcgt gatctgtttt gattttagca gtcacgcttt
    16981 ttcatcgtga tttaatgctt tataacgaac atttattctc taacagtttt gaatttggtg
    17041 tggacgagat caacgaagag gctagtagct ttacgatgga taagtacgtt cctgtaaaaa
    17101 caggcgattt tctactagca aaatatattc ctagtggaaa atttgcatat tttggtgtaa
    17161 ttacgtcgca ggaagacgaa aaaatcagtt gtaaaagctt acttagttta gctgatagtg
    17221 agataccgac tgcacgtgtg tcaggagata actacgaaga gcatatccgg cggttgattg
    17281 aatactattt gctgaatgat ccaacgaaac aactaaaaga tattttagac gtcaaagcag
    17341 aaagtgcgac ctctcattcg tatcaagcta ccgatacgaa taaacacaag ttaagtgcgt
    17401 atattctcaa tggtttcaaa aaatacaacg taaaatggta tttcaaggga attcaaaaca
    17461 gaaaaattta tacaggcata cgtgctgtaa atgaatcaat ttacattaaa gacaattctt
    17521 ctgaatttag cgattgggat gtgtttgttc aggcgccggg cgctggaaac gagaacaagc
    17581 tattaatcgt tgataaagca atgaaagata tagagaaacc gataatactg tcaacatggt
    17641 atttggacga agaaaacaat ttgacacaag atggatcgaa agaaaatatc acgaaaccaa
    17701 ctgtcaattt ggttaatatc tacgatcaaa ccgcagaaga taaagcatcc tacgaagatg
    17761 tggcaaaatc agagttgaaa ggcaatacgt attcgcatga aatcaaagtg aacgttgtaa
    17821 gaaatgcaaa aaatttgaat gtcgaaacga ttgaaacagg gatgtttgcc acgatttctt
    17881 ataaaggaaa gatatacaag tcggttttga cagcttggcg aatatcaagt gataaggagt
    17941 ttgtggaatt gactttcgga aacataagaa gtcgttttat ggattatttt gaagataatg
    18001 ggggataaaa aatggttagc aatgtggatg gatatcaatt tgaaaacgtg aaagtaagcg
    18061 cagaaaatga tgctagactt tatcacgttt tatataatcg gaaaaatcag gttattgatg
    18121 gttacgatca gtctatgaat ttatcttcaa gcggattaac agtaaaagtt gctgcaggag
    18181 cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga agaaagtata acagttccag
    18241 caaactcaag tggttatata gcattaacag tggatttgac acaagaagtt atacctggat
    18301 ctattcttcc agaatcggaa gaatatgaat ggactaataa tcaagtcaag ctagaattta
    18361 taacaaaagt tataaaaggt aatttaaaca atggtgataa ggtctataat ttaccactat
    18421 gctcagttaa ttctactgga tcaactgttt caatctcaaa gatatcggat agttacgagc
    18481 tgactctctc taaaggagaa attttgtgga gggggactgc gttaatgcat gatactcaaa
    18541 ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg ttttttgtta gtatggctcc
    18601 catacgaaaa cgggcaagca attgaggata gatatgtaac tacgcctttt tataaggagc
    18661 gtgtaacttt tactaatgtt ttaggagaaa ttgtttcagg atttgatgac tatcacaaaa
    18721 agtggtttag taaacgaata aactataatt cgaatactaa tatatttaca ggtgcagcaa
    18781 gcaatgcaag tggagataac gcaaatatgg tgcttaggta tatagtttct ttttagttag
    18841 gaggtatgga agagtggcaa atttagaaat taaattatct gcaaataaga gacagcctta
    18901 tctacgtcac cgtgttgttg gtagagttgg tgatgggggg ctcacaacaa tcaatgtaca
    18961 acttcttgag gaagatgaaa ttacaccttt tgtaattaat ctaaacggta ctttgaaatt
    19021 tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa ccagaaataa tcgattcgac
    19081 taatgggtta attagttaca cgttcactaa gtcaaatttc agcacgagtc atcaattcaa
    19141 acaagcatat tttgaatatg tagatcctaa cggcaaaaaa gtaacttttc agaacttcat
    19201 catagacgtg ttagaacgag tggatattaa ttcggagcaa gcgaaatact atatttcttc
    19261 attggaaaaa ttacagagtg aaatgcaaac cactttcaat cagttcatta gtgataaaca
    19321 ggtccaatac gatcaaatct actcgaaata taacgaatta gtaagattga taaatgaatc
    19381 agataagcaa gtaaatgatc gtattgatca aaccaatcag caaatcggcg atctcggcaa
    19441 gctgaaaaag atgtacagta acagcatcga ctttgggggc tatgattatt cggggaatcc
    19501 gaatttgtta agtaagctat catacgactt aattgaaaat caaaatactt cagctggaac
    19561 actttctaag ggtgaaaact cgtttaaata taataagata tcagctgaag tggaaggtgg
    19621 agtagagtta tattataaac gaagaggtat agctaactgg ttaccctcta ataaaacgct
    19681 tgtaatgact gttaagctta gagctggagc ggactatagt ccagttgacg gaaaacttat
    19741 actgattaga tataggtatg ttgacagtgg aactggcaag attgttttag acttacctat
    19801 taacagtaat tcgataactc aggaatggaa agagtttagt attactggaa ctactccaac
    19861 atttagccca caagcatacc atccttggat acaatttagg gctcaagatg gggtacttgg
    19921 ggaaatagaa atgagctatg acatcaaaat cgaagaaggc tctacagcta caccatatca
    19981 gcctaactta cttgatgact cttactggct aggtaaaaca ccgttgggtg agaatttgat
    20041 aacgaatgcc aagttcccaa ttataacacg aagcaatcct atttctggtt ttgatatttc
    20101 agaagaatta ataataggtg aaacttatac ggtatcttta aaagggacta agccagctac
    20161 taaagaattc cacttatact acggagaagc aaattaccag cagttgcagt atcaggcaac
    20221 tttacttcct gtggaaggat taacagatgt gtggagtgcc acttttacag ccagaaatac
    20281 tagtgaaacg acaaatttac ttagagtagc gttatggcaa aaacctaatt ctgcaacata
    20341 tgatactgtt caaattgact ggctcaagat tgaaaaaggc gacacacgca caccaaatat
    20401 cagtcagttt aaatactttg gtgaaggctt gaaagacagt aacaatccga acgactacag
    20461 ctgggatgtc acaccagagt atactgaaaa aagcttgaat aatacggtta gtctgactga
    20521 accgcaatcc atcgaaggat tgaaaaattt cgaagatggt attcaatcaa aaggaaaatc
    20581 tgtattgaca tcagacgaca acaaatatga agtcgtaacc ttaacagtta caaacgggaa
    20641 taccggatca gcaaagcttt atcgtgaagg aaaaaccgtc actatttatt tttttgcgtt
    20701 gaatgggaaa agcagtggtg gaaatgattc aacgatacta acaattccag aaggctatcg
    20761 gccaccaatt agttttgagc aactggttgg ctcgatagac cgttctactt tgaacagtgc
    20821 tcagttatct attggtgcag atggagccat taaatggcga agaaactcaa gttatggatc
    20881 ggattatacc tttgcaatta cttacacgat ttagaaaggc gtgaatcgat atgaaggcag
    20941 catatagacc aattgaacct tacggattcg agcaaatcat tgtgaatgat gaagaacatt
    21001 taccggaaga atgcacagaa gtcgaaccac cgattccaaa ttggaaaccg aaattcaatt
    21061 actgggaggg aaataaatga aaaacatttg gaaatatggc cgtactggcg gagagtatgc
    21121 aggaaaagta ttggacgaca tgcttgtatc cgttccttac acagatcagc caccgctcga
    21181 aggggttcgt tcagatggcg aaccgctaac gattgctgat cagatgtttg atcctaaatt
    21241 gaaccaatgg attattttag cgaacgcgtt agatcacaac gatttaaaca atctcaaagc
    21301 gatgtacgag gctcttgaac atgaaaacgg caacctaaaa cagctaaatg ccaaactcat
    21361 gctaagcgat gtagcgatta aacaggaaaa tactgcattg aaagaaaaag ctgacagttt
    21421 agcacaaatc aattcaaaaa tgatgcttgc ttcgttacaa aatagcaaag acatttcaga
    21481 aattaaagag caactaaatc cagcttcaaa gggaggtgag tagtatgttt agttttagcg
    21541 atgtgaaaat gatgtatgat tggggctgtt ttactgacga tcaagttcgt ctattcgttc
    21601 cactatgtat cacagatgaa gaagcagata gaattattag caaagaagag agcgcatctt
    21661 aagtgatgcg tttttttgtt ggaaagttgg tggaacatga aagaagaagc gctccaagac
    21721 gttgtggaga gattagtaag aattgaaaca aaattagaca actacgaatc acttagagaa
    21781 aaagctgata gtgcaaaaga tttggcagac aaagcctact cagtagcgct aaacaacgca
    21841 gaagatatca aagaaatgaa agccaataat aaatggtcgt ggggttacat gatcggttta
    21901 ggcattacga tcattggcta tttcttgact aaattgtaaa ggagttaaga agaaatgatt
    21961 ttacccgata agtattatca agtcattaaa tggacggttt taacagtatt accagctgca
    22021 tctgttttag tagccacgtt aggaaaagcg tatggatgga atggaacaga tatgacagta
    22081 ctcactatca atgcagtagc aacattttta ggcgttatca ctggtgtgtc tgcatataac
    22141 ttaaaaaaat aggaggaaaa aaatgaagaa gaaaatcatt ttatcattga gcctaataat
    22201 ggctcttttt ttattgccaa ttaacgggtt cgcctatacg atcaacaatg aatttaattt
    22261 aggtgtaaat gaaggtagct c
//
LOCUS       DAHUVV010000038        22159 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.038, whole genome shotgun sequence.
ACCESSION   DAHUVV010000038 DAHUVV010000000
VERSION     DAHUVV010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.038"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            229..2064
                     /gene="tdc"
                     /locus_tag="NK767_001469"
     CDS             229..2064
                     /gene="tdc"
                     /locus_tag="NK767_001469"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCH6694469.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2369..3847
                     /locus_tag="NK767_001470"
     CDS             2369..3847
                     /locus_tag="NK767_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6694470.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3813..5144
                     /locus_tag="NK767_001471"
     CDS             3813..5144
                     /locus_tag="NK767_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6694471.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5258..7810
                     /locus_tag="NK767_001472"
     CDS             5258..7810
                     /locus_tag="NK767_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HCH6694472.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7817..9256)
                     /locus_tag="NK767_001473"
     CDS             complement(7817..9256)
                     /locus_tag="NK767_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6694473.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9602..9964
                     /locus_tag="NK767_001474"
     CDS             9602..9964
                     /locus_tag="NK767_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCH6694474.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10151..11008)
                     /locus_tag="NK767_001475"
     CDS             complement(10151..11008)
                     /locus_tag="NK767_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCH6694475.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11128..12039
                     /locus_tag="NK767_001476"
     CDS             11128..12039
                     /locus_tag="NK767_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCH6694476.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12046..13692
                     /gene="mdcA"
                     /locus_tag="NK767_001477"
     CDS             12046..13692
                     /gene="mdcA"
                     /locus_tag="NK767_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HCH6694477.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13794..14093
                     /locus_tag="NK767_001478"
     CDS             13794..14093
                     /locus_tag="NK767_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HCH6694478.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14083..15759
                     /gene="mdcD"
                     /locus_tag="NK767_001479"
     CDS             14083..15759
                     /gene="mdcD"
                     /locus_tag="NK767_001479"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HCH6694479.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15763..16356
                     /locus_tag="NK767_001480"
     CDS             15763..16356
                     /locus_tag="NK767_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HCH6694480.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16380..17456
                     /locus_tag="NK767_001481"
     CDS             16380..17456
                     /locus_tag="NK767_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCH6694481.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17481..18350
                     /gene="mdcB"
                     /locus_tag="NK767_001482"
     CDS             17481..18350
                     /gene="mdcB"
                     /locus_tag="NK767_001482"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HCH6694482.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18461..19225
                     /locus_tag="NK767_001483"
     CDS             18461..19225
                     /locus_tag="NK767_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HCH6694483.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19714..21807
                     /locus_tag="NK767_001484"
     CDS             19714..21807
                     /locus_tag="NK767_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCH6694484.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21832..22134
                     /locus_tag="NK767_001485"
     CDS             21832..22134
                     /locus_tag="NK767_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6694485.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7177 a         3785 c         4721 g         6476 t
ORIGIN      
        1 tttttttagg ttatctattt tagaagactg atttattgaa aaattgttcg cttttttgcg
       61 ttgaattatc agaaaaatcc gactttattt atttttgttt gtaaaaatct acgtggattt
      121 gatgttataa aaaaaaaatt ctcatttttg gcgctatagt gaagaggtgt tacagataaa
      181 cacatgtatc tgcaagacat tgaccaacaa aatatggagg gaagaaaaat gaaagatatg
      241 gatatcaagg ccgtctttat aggcgacaaa gctgaaaatg gaccagttta taaaatgttg
      301 ttgaacaaga tggtagatga acatcttgga tggagagaaa attatattcc ttcagatatg
      361 cctgcaatta gcgaaggaga taagctcact ccagattatc tagccacccg cgaccatatg
      421 atagaggtct tggatgaagt cagtcagcga ttacgcgcag gatcgatccc atggcattca
      481 gcaggccgtt actggggaca aatgaatgct gaaacattga tgcctgcttt attagcttac
      541 aactatgcga tgctgtggaa cccaaataat gttgcattgg aatcttctat ggcaacttcg
      601 caaatggaag cagaagtagg acaggatttt gcttcattat tcaatatgac agacggatgg
      661 ggacacattg cagcagatgg ttctattgct aatcttgaag gattatggta cgcccgttgc
      721 atcaaatcga ttcctttagc tgtcaaggaa gtgctgcctg aaaaagtgaa aaaaatgtct
      781 gagtgggaac ttttgaattt atctgttgaa gaaattttgg aaatgaccga aagctttacg
      841 gacgaagaaa tggatgaggt caaagcagct tcttcccgta gcggaaaaaa tatccaaaga
      901 ttagggaaat ggctggtccc tcaaacaaaa cactattctt ggatgaaggc gttagatatt
      961 tgcggagtcg gcttagatca gatggttgct atcccagttc aagaggatta tcgaatggat
     1021 attaatgctt tggaaaaaac aattcgtgaa ttagctggac aaaaaatccc aattctaggt
     1081 gtggttgctg tcgtaggaac gacagaagaa ggtcaagtag acagtgtaga taaaatcgtc
     1141 caattaagag aaaggttaaa agatgaaggg atctatttct atttacatgt tgatgccgca
     1201 tatggcggct atgcgcgttc actgtttcta aacgaagcag gagaatttgt gccgtatgct
     1261 tccttagctg aattctttga agaacatcat gttttccacc actgcgtaac gattgacaaa
     1321 gaggtatatg aagggttcag agctatttca gaagcggatt ctgtcacgat agatcctcat
     1381 aagatgggct atgtccctta tgctgctgga ggaatcgtaa tcaaacataa aaatatgaga
     1441 aatatcattt cttatttcgc gccatatgtg tttgaaaaat cagtaaaggc tccagatatg
     1501 ttaggagctt atattttgga aggatcaaaa gctggagcaa ccgccgccgc agtctggaca
     1561 gcacatcgtg tattgccatt gaatgtgacc ggatatggtc aattgattgg ggcttcaatc
     1621 gaggcagctc aaagattcag agaatttctc gatcatctga cttttactgt taaaggaaaa
     1681 acaatcgaag tctatccatt gaatcatcct gattttaata tggtcaattg ggtattcaaa
     1741 gaacaaggct gtacagattt gaacgctatc aatgaattaa atgaaaaaat gttcgatcgg
     1801 tcttcttata tggatggcga tgtttatggt gaacggttta ttacttctca tactacattt
     1861 acacaagaag attacggtga ttctccgatt cgttttgttg aaagaatggg tctaacgaaa
     1921 gaagaatgga aaaaagaaca gaaaatcact ctgctacgtg cggctatcat gactccttat
     1981 ttgaatgatg atcgaatttt caatttttat acaaaaaaaa tcgccaaagc aatggaaaag
     2041 aaactaaacg aaatcatcca atagaactga tttttgtgtg aaagaataac aagagacata
     2101 tggagaacag acaattattt gcttcatcaa aacaattgta tttccccctg tttttcatat
     2161 attcctcgcc aagatattct tttttataaa acgttctata agttggatcg ttcgtttcat
     2221 gtatcaaacc cagttcacta agatcgttca atggatgttt cggtgatact gggttttgta
     2281 ctgaatcgat gaaaccttgt catttctgtt cttgaacaaa aataaaagaa aacaaaaaca
     2341 tatgtcgcta ttgaaagaag gaataaagat gaaagatact ggttcatcaa agcaaatcac
     2401 gaccatgacc tttatcggaa tgacctgcgc ccttgtagca agtgtacgaa atattccaga
     2461 tgtagcagca acgagctgga cgatgatttt ttacatgctg gttgcagtat tattgtacgc
     2521 ttttccaatc tcattgatct ctggtgaatt tgctggaatg tttccccaaa aaggtggtcc
     2581 agaactttgg gtatcgaatg ctctaggaaa aaaatggggg ttcgttgtct catggctgtt
     2641 atgggtccaa atgtttcctg ggatggtgat ggtcgcttca gcactcgctc cgttgtttgg
     2701 taatattatc gataatgtgc cgcttggttt gaacagcaaa tttacattag ttgtcatttt
     2761 agtcgtttat tggatcatca cttttttgaa tttgaaattt gatatggcaa aaatcggcgg
     2821 aaaagtcggt gtttggttag gattgtacgt gcctttgacg atgatgctct tattagggtt
     2881 tgctgcgtgg atcaaagttg ggatcgtacc aaccggaaca ttaggcagtt tttcatggga
     2941 caaattaata ccagatacta cgactgcatc ctcatttgtt tattttgcgc cgatcatgtt
     3001 tatttttact ggtatcgaaa tgtcctctgt gtatatcact cgattggaaa atccagttaa
     3061 aacgtatatt cgcggagtat ttgccgcgtt aatttttata tttttcgtca atattttgaa
     3121 tgctttagtt gtagcaaatg tcgtaccaaa aggacaaatg gaattgaata atatcgcgca
     3181 atctatttcg atttattgcc aaattcttgg tttgccacat cttattgtga atctgtttag
     3241 tttacttgtc ttcattggtg tagcagtcca attatctgct tgggcctcag gaccagcaaa
     3301 gacagtgaca gaaagtgcta gaaaaggtgc gtatccacca aaattcaatt tttggaagac
     3361 gaatcaattt gacgtatcca aatccgttat tttgactcag tctgtcatta tctcaatctt
     3421 tgcacttttt tatttgctga ttcctggcgt caatcaagca tttttgatgc tcgtaaattc
     3481 aactactgtt atctactgta tcgtctatgt catcatgggg atcgcagttt tgagattgcg
     3541 ttatacacat gcaaagttga atcgaccatt tagaattgga aaaaaagaga aaaagagcaa
     3601 tcttggagtt tggactgtcg ttattgtttt atttgcggca atcggatttt ctgtcggact
     3661 gacgatgaaa gctggaacat ggatcaatct aattgcagtc acagccatca gtgtcatttt
     3721 gtttgtagta ccactctgta ttgaaaaaat aaagaaacct tcttgggaac aggaagtcgc
     3781 catgctgctt gaaaaagaaa aaggtgaact tgatggaaaa accggcaatg aaacggactc
     3841 taactaatcg aaatatccag ttaatcgcat taggtggcgc aattggaaca ggattatttt
     3901 taggttcagc aaaggcaatt gcatcagcag ggccagctgt tattctcgtt tatttgatat
     3961 gcggtttggc tatttatggt ttgatgcggg ccatgggtga tttattttta gcaaatagta
     4021 catttaactc tatcagtgat ttcgttgact attatttagg caaaaagtgg gcattttttg
     4081 tcaattggac ttattggctt tgctggcttg gagctggact tgcggaatta acagcaatag
     4141 gattgtatat ccatttctgg tttgtacatg taccagcttt gataagcgga ggtagcgcac
     4201 taatgatctt aatggcagtc aaccttgttg cagtcaagtg gtttggtgaa ttggaatctt
     4261 ttttctcaat catcaagatc ttggggattt tcctttttat cttattgggt ataggattga
     4321 gtgtctcaga ttttcatacc cagtctatcg gaatcaaaga aaattttgga cagatcgatg
     4381 aattactacc gtttggcttt gctggaatgg tagtctcatt tcctatggtt ttattcagtt
     4441 ttgctggaat tgaaatgatc ggcttaaccg ttggcgagac aagtgatccg caaaagaatt
     4501 tgaaaaaagc aatcaactgt ctgccttggc gtattctctt tttctatata ggaacgattt
     4561 tgtcgatttt atttgttgtt ccatggcatt gtttagattt aaaagaaagt cctttagtga
     4621 ctatgctgca agtgatccat tgccaaggcg gagcagcaat tgtcaatcta atgattttgg
     4681 ttgcttcttt gtcctcagca aatagtgcag tctacagtac tagcaggatt ttatatcaaa
     4741 cagcaaaaga aaaaagtttg ccccattggt tttgtgcgct ttctaagaaa ggcgtacctt
     4801 tacacggtat tttaattaca ggaatgttat tttttgccgg tctttgtcta catttttttg
     4861 tagctgatag tcgattgttt tttcagcttt tggcaggcat gacaacgagt tgttttctat
     4921 ttgtgtggtc ttcgatcttg tgtgcacatg ttcaatttat taaagaaaag aaacggaaca
     4981 ctacacaaag atataaagat cttgctttgt tggtgttttt tacttcaatt gctttcagct
     5041 tgttattcga aagcacgaca cgctttattc cttttttatt ggttatctgg tttgtattct
     5101 taaattttgt atataaaaga ataagcaata gaaatatcag ttaggcccag atacattttg
     5161 caaaacgaat tatttcagac ccccctgaaa taatcgatac gcctgaaaaa acttccctgc
     5221 gtataataac agtatcaatt aaacaggagg ggataggatg aactggtaca gattgacggt
     5281 agaagatgta ttgaaaaact atcaaacatc aacagaaggc ttgtcgcaag aagaacgaca
     5341 gaaacgcctg actgatcaag gatataatga aataaaagca aggcagcaga caaaacgatg
     5401 gaaaaaaata gcaaagcatt ttactgatct gctgatgatc gtgttgatca ttgcctcttt
     5461 tttgaaattt gcttctagtg aatatattga aggaagtatt attttattcg tagtgatcgt
     5521 caatggactc gttagttact ggcaagaaag aaaagcggaa gaatcattga atggtttgaa
     5581 gcagctgatg ggacaagaag cgatagtact atcaaacggg atgcaagaaa aaatccctgc
     5641 gaaagaactg gtaaaaggag atgtcattgt cttacgtgcc ggagatgtcg ttccagcaga
     5701 tatccgatta atcgaagcac atgatttatt agtggaagaa tcgattttga cgggagaatc
     5761 agaagcatct gaaaaaagtc atgccgtttt gacagaagaa gaatcgatcg gcgaccagaa
     5821 aaatatgggt ttttcaggaa cgcttgtaca atctggttct gcagtcggtg tcgttgtaga
     5881 aacaggaaac acgacagaaa tcggtaaaat caatcaagca ttgcaatcgg tcgaacaaca
     5941 aacgacgcct ctgattaaaa aaatcaaccg attaaacaaa caaatttttc aaggcattct
     6001 ttgtttgagc ctattcttag ttatttttac gacatttcga tatggtatgg attggcatat
     6061 ccttctttcg gctacgattg ctttggtggt gtcaatggta cccgaaggat tgccagctgt
     6121 tttgaccatg attctatccg ttggagtaca tgaaatggca aaagaaaagg cgatcatcaa
     6181 aggacttcct tcggtagaaa cattaggttc tatgacagtg atctgttcag ataagacagg
     6241 aacattgaca aaaaatgaaa tgacagttgt ggatgtagct gcaaaagaag aagcctgcgt
     6301 gttgtccatt atgaaaaact gtcaggaatt aaaaacaaaa gaagaacaga aaacagaaaa
     6361 tttaagtggc aatccgacag aagttgcgct gattcgacac acagaaacag ccaatctacc
     6421 tttgaaaaaa atagaagcaa agattccgtt cagttctgaa tacaaatata tggcaacgat
     6481 gcatgcggag gaaaaaggag cggtgattta cgtcaaaggt gcaccagaag tattatttgc
     6541 taaatcaact ctttcttctg cagaacaaga agcatggagt cagacagctg cagaatttgc
     6601 gagaaaaggt cagcgtgttt taggatttgc ttataaaaaa gtagattcga aacaggaact
     6661 cacgcatgag actttaaccc aattaacttt tgcagggatc gccggattga tcgatccgcc
     6721 aaaagaaagt gcagtcaaag ctgtaaagga atgccaacaa gcagggatct cagtgaaaat
     6781 gattacaggg gatcacaaag acacagcaaa agcgattgcg gaacaaattg gcttaaaaca
     6841 tactgccaaa gttttagaag ggatcgattt ggatcttatg tctgatgaag agttgattca
     6901 gcaagtgcca atagtcgatg tctttgctcg aacgacacca gaacataaac tgcgaattgt
     6961 taaagctttg cagaaaaatg gagaaatcgt cggaatgacc ggagacggcg taaatgatgc
     7021 acctgctttg aaaagatccg atgtaggaat tgctatggga atcaaaggta gtgaagtaag
     7081 caaacaggca gctgatatgg ttttaggaga tgataatttc catacgattg caaaagccgt
     7141 aaaagagggc cggcggattt tggataattt gcaaaaaacc atcaatttct tcttgccaac
     7201 tgcattagct caaggattga tcttgatttg ggcacttatg ctgaatcgcc cgctaccttt
     7261 gtccccagta cagattttat gggtcaatat ggtaacgaca atcactttat cttatgcact
     7321 agggttcgag tcgccagatc cagaaatcat gaagcgccca ccaagagatc caaagcaagg
     7381 aatcctatct ggttatcatt tgtttcggat catctatgtc tccctactga tcatggttcc
     7441 tgcctattta ttggctatgc agtttgaagg gcaaagctta cagcaaacca ttcttctgca
     7501 aaatatcgtc ttagctcaag ctgtttacat gatcaattgc agagagttgt ccaagccgtc
     7561 attgaaccaa ggtcttttgc aaaacaaagc gctttttctt tcattaggta tccttgcttt
     7621 attgcaaggg atggttattt ttctaccagt cggccagcag ctgatcggta cagtaagttt
     7681 aacgatgaca caacagttat tgattggagc aaatgtgatt cttctattct tagttgtaga
     7741 aatagaaaaa gggatcatga acaaactgtt tcgcaaaaaa gaaaaaacag ctagagaata
     7801 cagtcgctag ccgttactat aaaaaagttt tgaaagtgag ttcagtcaaa cgattgagct
     7861 catttttcgt tggaatagaa gaaatgtata caatagggat atctagagaa tctagaggga
     7921 aattggaaat caagatatct gttttttcaa aagaaatagt ggaaagatcg gttagttcga
     7981 taggtaagaa gcagacacgt tttcctaagt aatcttttaa ttcctgctgc aagaacgcct
     8041 tccatgaagg ttctccttgg aaaataaaat agacattgaa caaattcggt ttgatagaaa
     8101 aaatcgtttg ttggatcagt agagacaaga gttggagaaa gtaatttggt tcattgattt
     8161 ctatttcttt ttttagatcg ttcagtaaat tctctttctg ttgatacaag tgtgcatatt
     8221 gctgatcata tgtttctaaa aaacgatggt attgacggtc caatgcttca atcaatatcg
     8281 gcgattcatg atattttaat atgaggagag ccaagttatc agtcagagat gtttttttta
     8341 gttgatcaag agacagctct gaagctagtt cctctacaaa cgtttcgagg acctctaaaa
     8401 atggagaatg atgcaggcag cgagcggttt cttgtccata gttattgttg taattccaac
     8461 tttccagaaa gcagaaagaa gcaaaagaaa gctcagtgtc tttaagataa ataccttccg
     8521 ttaaatacaa ttttttcaat gcagagtcat acagggagga gagtgtgctg ttggtatgga
     8581 tagaagaatc taatcggcaa ttcgctttta tacggatcgt attgataaag aaccaacatg
     8641 ttccgaaaat ctcttctgtt tcaaccgcta gttcagtttg actgaggttt cttaaaaatt
     8701 gaaaataatg ggattcatgg atcgaataat cctcgaaaaa atagttgtta tgagtgaaag
     8761 gaatcagcaa tcgatgataa aaagtacgaa tcgcacgctc ttttccaaga atacaagctg
     8821 tatatttcgt caaatcgatc tgcaagtcgt aacttgctaa acgctgattg attttagtca
     8881 catgtctttt caatgtccca taagaaagcc catggtcggc tagaaaatca gggacagcaa
     8941 ctttttttgt aaaaagcaga tttttcagca gttcgacact gactgattgt ttgaaaaaaa
     9001 tctcccaaag gtcatttgtt aataaatttt gctggttttg caaactgatt cctgcttgat
     9061 cgctgatgat gtcccagcct tcgggcagct gagagcgaat agattgaagg tcagaaaaaa
     9121 tcgttcgttc ggttgtccct atgatttcag ctaattcatt tacggttatc tttgtatgat
     9181 tcagcaattg ttctactaaa gaaacatgac gtcgaatatc tttttcggta atgatttttt
     9241 tcattagtga atacatcaat cagactccct tctattctaa gaatacggag aaaataaacg
     9301 gtggtcaagt ttcaactgat ttttcaatca ttgtttattt ttctggagca tttactgatg
     9361 ataagaattt catcatgaac ttgaaaaatg ataccactaa aatcaaaaaa taggtgggca
     9421 aacaataata aacattttaa tagtagatgg tagcaagacc aacacaggcg gattgaacat
     9481 ttaagagttg ataaaaaaga aaaatatcga agtatttgat tagcaactac ttgtattcat
     9541 ttttctattc ttacgtggta tctttgaata aagcgaatca ataagaaaga gatggaagca
     9601 gatgctaacg aaaaaagaac taatcgatca catcaatgaa acatacaatt tgtctccaga
     9661 atatattttt aaaaaatttc ctgattattg cgtattcaga catcaaaaaa gcaaaaaatg
     9721 gtttggggtc ctgatgtctg ttccagaaga aaaaatctat gatgatgcaa gtgaccatag
     9781 gatcatcaat attcttgatt tgaaggtcga tcctgaactt ggcggaattt tgcgaggaaa
     9841 agaggggtat tttccagcct atcacatgaa taaagaacat tggatctcta ttgatctacg
     9901 taaaatagaa aagctagatg aactagcaga tttgattgat ggaagtttta aatcaacgca
     9961 atgaatgtct aggacaatag agaagaggtt gtgacagaaa tgtttagctt caagaaataa
    10021 gaaggaattt acgaaaattg cttctcaaat ttttgggaaa tttcggctta ttttctaaga
    10081 aattacttct ggaacaccgt ttgttcgaaa aaagagctgc gacaagaatt ttgtcccagc
    10141 tctttttttt ttattttcct aaataaaatt cccgcatcgt ttttgcaatc attttgatca
    10201 aaggatgatc ttctgtgaag ctagaagata aataaattgt tcgtgtcaag ggcaaatctt
    10261 tgatttcccg caagacgaaa ggatcatttt tatcgtaaga ccaagaaact tttgggaaaa
    10321 agctaacagc taatccttgc ttgacaatag aacgtaatgt cgcaggatca tctgtcgtcg
    10381 aactgatcgt caattcgata cttttcgctt caagtgctgc atccagtgta tgacggaagg
    10441 cgttgctctt tgacagcatc aagatggggg tgcgttggat atcttctaat tgcagatgtt
    10501 cttttttagc aagaggatgt ttcttaggaa tggcgacata aacaggttct ttcatgatag
    10561 gaatattggt catgccaggc ttcatctccg aagtgatgta cagtacagga tcggtaatat
    10621 catctggtaa atgatgttgc atgatcgtta attggatatc aggatgcttt tccataaaaa
    10681 ttcggaccaa gttcggaata gaaacagagg ctacctctaa atacaatatg acggaagtaa
    10741 cgatcttctc attgaccgaa gacaataatt tgattccttc ttctaatgtg tctaatgtct
    10801 tgactgcata tggataaaat tcttctgcat aaacggtcgt ctcaatgttt ctgccgctat
    10861 ggtaaaacaa agggacacct aattcgtttt ccaaatgttt gatcgcacgg ctcaaggcag
    10921 gttgagaaat aaataattct ttggctgctt gggtaacatt ttttgtttcg aatacttttt
    10981 taaaatactg cagatgaagc aatttcataa ttatccatcc catccatacc tttttgttat
    11041 ggttttttct ttttttggca ttttttatta tagcagaaat tgactaagat agcagtggaa
    11101 aaagtaagtg ctgaggaggc gaggctcatg agtacaggtt tcttatttcc tggacaaggc
    11161 agtcagaagt tagggatgtt agcggatatc cctgaaatgt acatggaacg tttagaaaat
    11221 gtgacggggt atcggttagt tgataaagaa gaaaactatc aagatacggt ttttatccag
    11281 ctagcattac tgacaaaagc agtattttat ttagatgaaa tggaaaaaaa cggtattcag
    11341 cctgatattg cagcaggaca ttcgattggt gctttttgtg cagctgttgc ttgtcgatct
    11401 ttgagatttg agaatgcagc ggaactagtc tatcaccgcg cagtcttaat gaaagaagct
    11461 tatccaagcg gttatgcaat gggcgtagta gtaggtgtga cgagaagcga agctgaggaa
    11521 attgtagcaa aaacatttga tcctgaccat ccagtttatc tgtcaaatga aaattgtccg
    11581 atgcaacata cgttatctgg gagtatcgaa ggtttatccg ttacgttggc agaagccaaa
    11641 cggcaccaag cgcaaacagc gaagctgctg aaagtccctg tgccatctca ctgtctcttg
    11701 atgaatgata cggtcaagaa attcaagccg tatatgcagt ctgtccatct atctgatgct
    11761 agatgtttgt atataaaaaa tacggatgga agagccactc aggacgcaga agagattcgt
    11821 aaagatttgt tggaaaacct agctttccct gtgcagtgga accaaatgat ggacttggca
    11881 aaagaattag gaatggatct gacgattgaa tttccccccg gcaatacttt gacaaagctg
    11941 attcatacaa aatttggtgt agataaaggg atccgtacga tcaatctaga ccaacatggt
    12001 attgatgacg ccacgtttct atatcaaaaa tggagatgac taattatgga aaaaccaaac
    12061 cgcaactggg cttctaaacg cgaaaaaaaa gcgcagcgat tggaaaaaat cagcaacttg
    12121 ctcaatggca agtatgtaga aactgaaaaa atcgtagaag taatggaaaa actgattgct
    12181 cccggcgaca aagttgtatt agaaggcgac aatcaaaaac aagcaagttt tctttcagaa
    12241 agtcttgaaa aagtagatgc agaaaaagtg aatgatctgc atttgatcat gtctagtatc
    12301 tcacgaccag aacatttgaa tatttttgaa aaaggaatcg caagcaagat cgatttctct
    12361 tatgctggtg cacaaagctt gcgtgtcgcc caaatgattg aagacggcac aatgaaatta
    12421 ggtgatatcc atacttattt ggaactttat ggacgcttat tcatcgattt gattccaaat
    12481 gttgttttag tagctgcaga taaagcagat aaagacggca acttgtacac aggtttcaat
    12541 acggaagaaa caccaacaat catcgaagct gcagctttca aagatggtat cgtgatcgtt
    12601 caagttaacg aagttgtcga ttctttacca cgtgtagata tccctggtga ctgggtagac
    12661 gttgtcgtga aagcagataa accttatcaa ttagaagctt tattcacacg agatcctcaa
    12721 aacatcacag aattgcaaat tctaatggca atgatggcaa tcaaaggaat ctacgcagaa
    12781 cacggcgtac aatctttgaa tcacggtatt ggattcaata cagcagctat cgaattgtta
    12841 ctgccaactt atggtgaatc actagggtta aaagggaaaa tcgcgaagaa ctgggtattg
    12901 aacccacatc caacgatgat tccagccatc gaatcaggtt gggttgaaag catccatagt
    12961 tttggtggcg aagttggtat ggaaaaatat attgctgctc gcccagatgt cttttttgtc
    13021 ggtaaagacg gcacgatgcg cagtaaccga acattatcac aagctgcagg acaatatgcc
    13081 atcgatatgt ttatcggttc aaccttgcaa ttagattacg aaggaaactc ttctaccgta
    13141 acaaaaggac gtttatctgg ttttggcggt gcgcctaaca tgggacataa tcctggcggt
    13201 cgtcgtcatt ctacaccagc atggcaatca cttcgcgaca atgatgatcc attagggaaa
    13261 ggccaaaaat tagttgttca aatggttgaa acatatggtt caaataaaaa accagtcttt
    13321 gtcgaagaat tagatgcact agctgttcaa aaacaagcag gcttagccaa tgcaccagtg
    13381 atgatttata gcgaagatac tacacatatc gtgacagaag aaggaattgc ttatttgtac
    13441 aaagcaaaaa caaaagaaga acgacaagca gcaattgcag caattggcgg tgttacacct
    13501 cttggaatga ctagcacgaa agaatcacta gatagcttgc gtgcagccgg catcgtgaaa
    13561 ttaccagaag atctaggcat caaacgtagt gaagcgaaac gttcattatt agctgcacaa
    13621 accatcgatg atttgatcga atggtcagat ggcctgtatg aaccaccaat gaaattcaga
    13681 acttggtcat aaaattggcg agaaaaacct tttgtcgcca tttacccaga tggaagaaga
    13741 aagacgaata agcagaacgt gtatgctctg ttttacaaaa aggggagaga tccatggaac
    13801 aattagctta taaatatcca acgaaacagc cgatcaataa gaaaatccat gtaggagtgg
    13861 tcggttcagg agatttggaa atcttgatgt ttccaacaga aaaaacagaa tctactgtaa
    13921 aaatttgtac aggaagcgat ggcttcggcg aagtatggaa caatgtatta acgagatttt
    13981 ttgatcgtta tccaatttca gcagatatcg tgatcaatga cttcggtgct acaccaggag
    14041 ttgtgtacct acgattgacg caagcaatgg aggaactttc agatgaaaaa tagttttgta
    14101 gaattaaacg ccagagaacg agtgaacgct ctacttgaca atggacgtga attgattgat
    14161 ccattcgatc aaatcatcgc gccaagttta ccagcacaag gaatcgtccc tgaaagtgat
    14221 gacgggatcg ttgtcagccg tggcacgatc aatggcaaac aagcagtagt aatcgctatg
    14281 gaaggaaaat tccaaggtgg cggtatcggt gaagtttccg gtgcaaaaat cgttgctgca
    14341 cttgaacatg tgttagaaga aaataaaaaa ggaaatgaga tctttccagt cattgtacta
    14401 gatacaggtg gtgttcgttt gcaagaagca aactacggac tgttatctat ttcagaaatc
    14461 ggaaatgtaa tcattgctct aaaacaatat gtaccagtca tcggtttgat tccaggacgt
    14521 gtaggatcat ttggcggtat gtcgattact tcagcattaa tgagttattt aatcgcaaca
    14581 aaaaaagcga gagtcggttt gaatggacca gaagttattg aacaagaagc aggcgttcga
    14641 gaatttgatt ctagtgacaa agacttgatt tggaatacat taggatctcg tcaacgggta
    14701 gaatcaaaaa tcattgatga attagtgact gatagtgtag aagcaatcaa agctgctgtc
    14761 atcgcagcaa tggacgaacg aaaagacacg catcgcagtg aaagctcagc attttattta
    14821 tcattacttg attctttaga tctttcaaaa ccaatggcca ttgacgatta caatgaagca
    14881 attgctaacc atcaagcaaa atcaattgac ttaccagaag tagcagaagg aaatgaacca
    14941 gctgcagcaa gtgttggtta cgattggttt gcggcactaa ctggcatgaa aaatccaact
    15001 tcttctatta gtactgtgta ttatggtaaa tcaagtaaat tcatagaaga agccatcgtg
    15061 acatcaatcg tacctgatcc taaaaacccg atgtaccgtg tacgaaacgg tgaagttggt
    15121 ttagttgaag gtttccgcat gggacaaatt ttggatcacg tttacgaaga agataaagac
    15181 aaaaaagtga aacggccaat catcgttgtg atcgatgtac caagccaagc atacggctat
    15241 aacgaagaat tgatcggtat ccatgtggct ttggctaata gtgctgctgc ttatgcaaaa
    15301 ctccgtcaag ctggacatcc agttattgga ttgattgcag gaaatgctat ttcaggtgca
    15361 tttttagcac atggattaca atcaagccgc ttgattgctt taaacaataa agaaattacc
    15421 gtccaagcga tgtctaaaga aagtgctgca cgtgtgacaa aacgttcggt tgctgaaatc
    15481 gaagcagctg ctgaaaaagt tccagctatc gcttacgata tcaataatta tacaaaacta
    15541 ggagcgatct atcatttcct agaagatatc accgaccaag ctgcttcaga acaaaatgtc
    15601 agcaaagtca tcgaagcaat caatgaagcc attcaagatg tccgcaatac aaatgacacg
    15661 atgctgacaa atcgatacac aaacgaaaca gccaaaacat tcggacgtgt cgaaacaaat
    15721 aaagtccgtg ccaaaatgga cgaagaatgg gatgcataaa aaatgattca agcacatgat
    15781 attgtccttt ttgataaaca tgaaatagaa gggttcacct tgcctgaatg ggtcgttcta
    15841 actgaaaaga tgttcggaac agttcgtcgt gcgtcgacgg atcacgcaga aatactaccg
    15901 attggtttac gtggacctgc ccgcaatcag agatggtcaa cagaaatctc aaaacatgcc
    15961 gtcaaacaag tcattcatcc gtgggacatc atcgaacaag aatcgtatag acaagagaaa
    16021 atcatggatt atcctgttta tcagcaattt catgctgcta gaaacatatt atcaggatgt
    16081 aaatggggaa tcggcggcag cttaggattt gaattatcga ctgggattcc ggcagtaaaa
    16141 gaaaccagtg attttgattt gctgctgtac gcaaattcac cgattgaatt gccaattcag
    16201 gtaattcaat cgcatcctgc tttttttgag caattcgata cccaagtagt gactcggcaa
    16261 ggcggttttt cactaaaaga gtatttgaga gagccagaga aaaaaattct attgaaaaca
    16321 gtcgaaggac ctaaattaac caaagaactt tggtaataaa gaaaaagaga gagaaagaga
    16381 tgtttgaaaa caaaaaaatc aaaatcaacg taagtgactt ccagaaagta tcagctgtaa
    16441 cggctgttat gatgcaaacg atattgagct ttgctttggc aaacagccac agcgcaacaa
    16501 atacagcttt tcttggtacc ttttacgtca tcgttaaata cacagcacca atgtttattt
    16561 ttgcaattgt ttataatatg gtaaaaacaa gtcagcattt gtcttattgg gaatttttga
    16621 aagaaaagtt ttttgaacta gtcgttccgt atcttctatg gacgacggct tatctttggc
    16681 tatttccagc agtccaacaa caaactccgt atacggatac tgcaagcttt ttactgaaat
    16741 atgtcacagg tgatggagcg cctcatttat ggtataccgt tatgatgctg cagatccaat
    16801 tgttcatgcc gttttttgtt tggctcggtt acaaagtatt tgctaataaa aaatatgtat
    16861 ggccagtatt gattggttca acgattttat atgtcgcatg gtatctgttc tatcaaacac
    16921 aagtattgac tgggccttat catgacagct ggtacttatt agatcggttt gttgctagtt
    16981 tcatgattta tggggtagcc gcatttgttt atcacgaaaa agtctatcaa tatttggaca
    17041 gagtacgtta tcttttctta cctgttgcct tagttatcgc gttcttttct gtccgttcat
    17101 tgctcgcaca tccaggagac ttgagctttg cgaatgcacc ttacttgaat acgatccaaa
    17161 gtttatacag tttagtcatc atctttgctg tcttcatagg cgcttccaaa atgattgtaa
    17221 acgattcgcc aaaattgcca ttgttcaaat ggttgtctgt ttatgcttat cgtacgtatt
    17281 tagccaatgt atttgttttc caagtactgt tattattgtt taaagatagt tggctgcagt
    17341 taccttcagg agtcatgatt cttgtagctt atctaatgac ggcaagttgt gcatttgctt
    17401 taagctggtt acttcatatc atttgggtag ctatcaaaaa aggtttcagc aagtaatagt
    17461 aggaagaagg agtgagtgtc atgataaatg acgaacgaaa aaaagcagtg actcagcaac
    17521 tcgctgcgtt ggtagaggaa gcattgatcg cagaagtaac attaagtcca aaacctggat
    17581 tagtggatgc actggatgcg ggtgcacatt ccgatatgga ctatgcttta tttcttaagt
    17641 ctgctaagac gctaactcct ttttttgaag aaatggctca aatagcatgg catcatgcta
    17701 ttgatcagga gttaagagaa agaattgctg agattgggcg aaaagcggaa aaggcaatgc
    17761 tgattgctac aaatggcatc aatacccata aaggagcgat ttgggcaatg gggctattga
    17821 tcagtgtcat tgcacaaaaa atcagtatgg aacagccttt ttctttatcg aaaatacttg
    17881 atgatgtcag caagcttgct gcttttcctg atacaaaata tcgtcataaa aaaaccactc
    17941 atggtcatag ggtgaagcaa aaatatggcg taaatggtgc atatgaagaa gcgatattag
    18001 ggtatccgca tatcaggctt gcaattcaag cagctaagga gctagataat gattcatatg
    18061 tacagaaaca aatgcatatg ttgttagttt tgatgagttc gctagatgac acatgcgtat
    18121 tgcatcgcag tgatcagaaa acgttagtgg aaatgcaaaa acttgctcat gctgctagtc
    18181 aaagtgcttt accaaatcgt ccatttacag cactgttgca cttttgtcag gaaaaccaaa
    18241 tctctcctgg aggaagcgca gatctcttgt ctgcaagctt gttcatgctg aagatagagg
    18301 aattatggac tgccgacaca tacatttgtc cgatgttact caatgactaa ataagacaaa
    18361 attgctaaaa aaacagttta tgcatctggt tattgactct tccttttagc aaggctatca
    18421 taaaaatagt tttttgtaat aaaagataac gaggaagaca atgaaaaaaa tcaattattt
    18481 agcaatcatt ttgttagtgg gcctatgttt gtttgctctg ccgacccaag gattcgctgt
    18541 cgcgggtggt catggtggag gtacatctag cggtcgaagt tcgggcagag gttcatttgg
    18601 tggtcgttca tatgggggca gttattttgg cagtcgttct tacggcagag attcttatgg
    18661 gtattatggt ccaggtacag gtccttctag ttatgagcaa acgggatttc ttgcattagc
    18721 tgttttgtca gtaggagggg tttctgcttt ttctatcgtc aagaataaaa gacaaaagaa
    18781 aaggctgaaa gcggagatat tgtcccgcat gtcaggaacg gctaaagaaa agaaagcaca
    18841 gtttctggaa atccaaaaga tattcatgaa gatccaatcc gcttgggaag aagaaactcc
    18901 tgaaaaagcc agaagctgtt atacagacaa gctgtttgcc gaacatcaga aagttctgca
    18961 aaaaaacaaa gcagacggtg taagaaatca tactaaaaaa ataactattc aggaagtagg
    19021 aaattatcga cagatccacg aaaatagttt tgccatccga attgatttca gatgctgtga
    19081 ttattcagtc gatcgaaaag atgggcgatt gatctcagga agtaagacac aacgacagta
    19141 tttttctcag atatggtatt ttgatttttc tgagaaagaa aaaagatggc aagccgattt
    19201 tattcagccg attatgctgg attgacagga aatatttatg aacggagttg tgacaaaagc
    19261 cttgtcgcag ctcctttttc tgaataaacg gtgtgccaga gatagcctat cggcaataag
    19321 gcacgatttt gtaaaatatg ggaagcgatt tttcaaaatc gcttcttatc gctcaaggct
    19381 gaacatctct gtcacaacct ctccactaaa tggtgtttaa aagcttgcat ctgcgataat
    19441 aagaacaacc aaacgaaaaa taggaagcac tattttagtt tagttgttct tattacttga
    19501 tgcaggacag ctttttcaca acctcttttt tgtatagatg ggcaaaattt ttgtaataat
    19561 aggcgtatgt ctttttcgtt gaggagatga aaaagcatat ggcttatttt ttgtttgctt
    19621 tgcttacttt ctttccactt caagtggcag aaatatctac tttttattat gtaagcggaa
    19681 ccatatactt gaaatcagaa ggagatcatt tctatgaaaa caagaacaat tgaaatactt
    19741 cacagaatca tgacagccca atatccagta aaagcaaaac atctagcaag tgagttcaat
    19801 gtcagtcagc ggacgatccg tcaggaagtt cttgaagcga atatctggtt gagcagtaaa
    19861 aagcttccgg agatccgtac aattcgcaac aaaggttttt tgttgaagct ggaaaaggaa
    19921 aagcaagctc gattagaaac tgaaataaaa gatgtgaaag acggcttttt gaatcggcgc
    19981 gaacgtattt ttgatctagt tttagccgtt gcttacagcc gaatcccgat ccatctaaac
    20041 aaaaaagaag aagaatttca aatctctaaa agtacattag atgaagatat gagacggtta
    20101 cgagcaacac tggtcagtta tgggatagag atcgtcagtt atggaaagca aggactggtt
    20161 tataaaggtt cagaacgatc tatccgtacg atgatctatg attttatcaa tcagaatctg
    20221 ggaatgaccg acttctttat gaatcaagat gcacaattaa caccggctca aaaaatcttt
    20281 ctccattatt ttcctttggt ggaaatcaaa aaaatacagg agatctattc caataagttg
    20341 ttgaaacaag aagatgatat ttataaaaaa caaatcctgc tttttacatt gatctggatc
    20401 cagcgaatca ggaaacatga atttatctct gccattaatt ggaaaaatac agaaataaaa
    20461 gacagtagct tttctgcatt tattgaccac atgatcgagg aatttcaatt ggacaatgtc
    20521 ccgcaagtag aaagaaatta tcttcgtttc actattgaga cgttcaatgc tagagatatc
    20581 tccaattcaa tcgagtgggt acaagctcaa ttactaacga tccaattgat ccaatttgta
    20641 gagaacgaga cacacgtacc tttccatcta aaggaagaag ctttattcga aaatctctat
    20701 aagcacatgg ctgcactgat tgtaagaatc aagaatcata tccaagtcat gaatccattg
    20761 aaagaaaaca tccgaacaaa ttatttccca atctaccgcg cagtcactca ttttgttcct
    20821 acgatcgaag aagtaattgg tggcaagata ctggaggacg agatagcttt tcttgtcatc
    20881 cacttttcga cgatcgccag tgtaatcaag cgagacttga attatgttta taaatcagtc
    20941 gttgtctgta accatgggat ggctactgga aatctgttag cagagaatct gaaagaaaag
    21001 tttcctcaga tcgagattac tgcagtttta agttcaaaag aaatcgaatt agtagataaa
    21061 ttggatgtca atctggtttt cagcaccttt caattgaatt atcgaaataa gccgctttta
    21121 gttgtcgatc cgattttaac tgagacgaat atcttggtta tagaagactt tttgaaaaga
    21181 aatgaagaac tacagcggtt agagcctaac agtcatgaat cgacggagtt attcactcaa
    21241 ataatagatg tcatgaaaga aagtggtggc cgagtagatc gaatcatcta tggcaagtta
    21301 gaaaaattat tcgaaacaaa caatttagaa atcaacaaaa gggagatcca gcctatgtta
    21361 aaagatattt tgacggacag caatatcatg atcaaagaaa aagccactac ttgggaggaa
    21421 tcgattgaac tagctgctag accattggtt aaagaaaaca ttgtcgaaga gcgctatgtc
    21481 gaagcgatga ttcgtgcagt gaatgattac ggaccgtata tcgtgattgg caaacacatg
    21541 gcactagccc atgcccgacc agaagatggg gtcaataaat taggagtcag cgtagctacg
    21601 attgagcaac caatcgattt cggtaatcct gaaatggatc cagtaaagat tattttttgt
    21661 ttggcagcag tagactccta ctcgcatctg aacatcatga aagaactaat tgaattgatc
    21721 aacaacgaag agaagttgga tcgattgatt gaatgcacaa atatcgaaga tttcaaacaa
    21781 ctgctttttt cagtagttga gaaataataa atgatacggg ggagaacaaa gatgaacaaa
    21841 ttgaacattt tattcgtatg cggtgcaggt ttaggaagta gttttgcagc acagatgtct
    21901 gctgaggatg ttttgaacaa attaggagta gaagccaaac tggaccattg tgatatttct
    21961 tccgcagttt ccatgaaacc agatgtgatc atcacagcag aaaattttcg ttcgcagttc
    22021 gaaaagttcg cagttgatcc taaaacggcg atgatctatt tgaaaaatat tgtttcaaaa
    22081 aatgaaattg aagaaaaaat caccccagtt ctgcaagata agggagttat ctaaatacaa
    22141 aaagaaaaga gggtaaaaa
//
LOCUS       DAHUVV010000039        21863 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.039, whole genome shotgun sequence.
ACCESSION   DAHUVV010000039 DAHUVV010000000
VERSION     DAHUVV010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.039"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(278..676)
                     /locus_tag="NK767_001486"
     CDS             complement(278..676)
                     /locus_tag="NK767_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCH6694486.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(673..1446)
                     /locus_tag="NK767_001487"
     CDS             complement(673..1446)
                     /locus_tag="NK767_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCH6694487.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1443..2126)
                     /locus_tag="NK767_001488"
     CDS             complement(1443..2126)
                     /locus_tag="NK767_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCH6694488.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2293..3180)
                     /locus_tag="NK767_001489"
     CDS             complement(2293..3180)
                     /locus_tag="NK767_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6694489.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3177..4046)
                     /locus_tag="NK767_001490"
     CDS             complement(3177..4046)
                     /locus_tag="NK767_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6694490.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4183..5469)
                     /locus_tag="NK767_001491"
     CDS             complement(4183..5469)
                     /locus_tag="NK767_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCH6694491.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5742..6320
                     /locus_tag="NK767_001492"
     CDS             5742..6320
                     /locus_tag="NK767_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCH6694492.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6320..7249
                     /locus_tag="NK767_001493"
     CDS             6320..7249
                     /locus_tag="NK767_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6694493.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7246..8982
                     /locus_tag="NK767_001494"
     CDS             7246..8982
                     /locus_tag="NK767_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCH6694494.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9045..9881)
                     /gene="pheA"
                     /locus_tag="NK767_001495"
     CDS             complement(9045..9881)
                     /gene="pheA"
                     /locus_tag="NK767_001495"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCH6694495.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9878..10387)
                     /locus_tag="NK767_001496"
     CDS             complement(9878..10387)
                     /locus_tag="NK767_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCH6694496.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10380..11675)
                     /gene="aroA"
                     /locus_tag="NK767_001497"
     CDS             complement(10380..11675)
                     /gene="aroA"
                     /locus_tag="NK767_001497"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCH6694497.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11688..12779)
                     /locus_tag="NK767_001498"
     CDS             complement(11688..12779)
                     /locus_tag="NK767_001498"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCH6694498.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12796..13962)
                     /gene="aroC"
                     /locus_tag="NK767_001499"
     CDS             complement(12796..13962)
                     /gene="aroC"
                     /locus_tag="NK767_001499"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCH6694499.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13964..15028)
                     /gene="aroB"
                     /locus_tag="NK767_001500"
     CDS             complement(13964..15028)
                     /gene="aroB"
                     /locus_tag="NK767_001500"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCH6694500.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15061..16083)
                     /gene="aroF"
                     /locus_tag="NK767_001501"
     CDS             complement(15061..16083)
                     /gene="aroF"
                     /locus_tag="NK767_001501"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCH6694501.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16742..17716)
                     /locus_tag="NK767_001502"
     CDS             complement(16742..17716)
                     /locus_tag="NK767_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HCH6694502.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17697..19580)
                     /locus_tag="NK767_001503"
     CDS             complement(17697..19580)
                     /locus_tag="NK767_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HCH6694503.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19598..21823)
                     /locus_tag="NK767_001504"
     CDS             complement(19598..21823)
                     /locus_tag="NK767_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCH6694504.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
BASE COUNT         6238 a         4823 c         3881 g         6921 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaatagcat attttgaaaa gaaagtctga tgcttagagt agaatttcat aaaaatttac
      181 tatttcataa gtattcacga gaaaaataca aacaaaaaag cggaaataac gctcatccgc
      241 cttcttgttt gtgtttattt cctcttcaaa cgcttgtcta tgattcagac ttttctctat
      301 cgttattttc atctaaaagt tcttgcattc cgataatttc tcgatattga tgaatcttag
      361 tgataccaat gactttgtct acatcaatac taaaacaaat accattacct ggaatatcga
      421 tgttgatgcc ttgaccaatc gcatccatta ctttattcgt caaactatcg ggtactaatg
      481 tcaacacaat atctttttct ggcgaaatgt ccaaacctaa aaatttccct gaatcatgta
      541 caccagaacc tcttccatgt aagatcgttc cgcctgctgc acctgcttct tttgaaaagt
      601 cgataacatc ttcgcccatt cctccgtcaa cgatcgtcac aatcatgtga agattcaatg
      661 ctacaagatc tttcatttgc tttcccccaa tttttgcaat ctttcttttt cgattctgtc
      721 tttttcatct tttcgtttat aaatatatcc tagtactaaa gtggataata ttggtgtcaa
      781 tgcaatcatt gcaaccacac caaacccgtc aatgatcgga tctcttcctt caatttgtgc
      841 agcgattgcc acgctcattg ataaaataaa tgtcgaacac atcgggcccg ttgctactcc
      901 accgttatca aaagccattg ctaaaaagat cttgtccact tttcgtccta aaataaatgc
      961 tagcccgtat cctgggatca agaaataata aagagagaat ccgacgagca aacgccacat
     1021 agacaatcct actgctgcgc cgatcccaat tgaaattacc atcagcatga ttttcgatcg
     1081 gattctcccc tcgctcaatt cttcaacctg tttgatcatt acatgaatcg ccggctcagc
     1141 aaaccctact aagaacccca ttaaaaagcc aaggggaacc agtatataat tgtaaggcaa
     1201 tcccgcaata gcactgccta aatgctgtcc taccggtaca taagccatat tgactccgtg
     1261 caggaaaagg atcaacccaa tagtcgttat caagaaacct ttcataatgt caaagaaacg
     1321 ctttttgcgc atacgaaaac taatgatatt cattaaaaca aataggatta cgataggtgc
     1381 cactgccaaa atgacttcca tggtaacgat ttcgattcca tctatcactt gctgccagaa
     1441 actcattgga agatcacccc caaaatcatc acggcaagaa tcggccctaa cgaagcaata
     1501 ccgaccattc caaaactgtc attttcattt ttcttttgtt tgatcatact agtcatccct
     1561 ccggcaaggg ccaagataaa tggaacagtt actggaccag tagtcactcc ccccgaatca
     1621 aaagcaatcg gcataaattc ggaactggtg aaaaatgagg cgatcaaaac gcctatgtag
     1681 cctatagcca tcaattgata ataggatagc ttgaatacga cccgaagcag cgcaaaggct
     1741 agaaatactc cagtgccgac gctgacgatc ccaatcagca acactctgcc aatctctccg
     1801 tcagatatat catatacttg ttgtcccaaa acttgcacgg aaggttcagc aatcgtgatc
     1861 acgatcccga tagcaaatcc caaactgacc aaaacggtca aacttttctt tttgatcata
     1921 tatttcccta ctagatctcc gacttccatc atagaataat ctgctccaaa caaaaagagc
     1981 gtcattccga tcatcatgat tgccgcacca accagaaatg aagcgaggtc ttctccttct
     2041 aacggtgcaa aaacaaacgt taaaatgaca atcaggacag tcatcggcaa tatagcagca
     2101 atcacttctt taaaattttt tagcatgtat cctcctcctt tactctttaa gtttacctta
     2161 aaatgaaaga gttttcgagc acaaacttaa aacgttatca tttaattttt ctcaacataa
     2221 aataacacac gtcccaaaag tatcttttta cttggaaaaa tgactttgaa ggaacgcgct
     2281 tattgtgttt ttttatttta cagagccgat cattccttga acgaattgct tttgcaaaac
     2341 aaaaaatacg atggcagttg gtaaagtagc gatgactaat ccagccatga tcattccata
     2401 gtctggtgta taggaagcac ccatagcaga taaaacaagc ggaaccgtcc gtttatcagg
     2461 agattgcaaa gctactaagg gccataaata attgttccag ctactcatga atgtgatgat
     2521 tgcagccgcg gcataggtat ttttagccgt cggcatatac actctaaaaa aaatacttag
     2581 ttcttttaga ccgtctaatc gagcggcttc tactagatct tttggaaaag atttaacatt
     2641 ttgtcgaaag aagaagatca aaaaagcagt gctaacagat ggcaggatga caaccaagaa
     2701 actgttgatt cccaaaaccg ttccgttcaa ttgtgaaaac attcgatata aagggatcat
     2761 caatgccgca aaaggaacca tcatcgatag cagtagaata ttgaaaatta ggtcttttcg
     2821 tttactttga taaatctcaa atccatatcc tgccattgac gaaatcagca atgcaaacac
     2881 agtggtcacc aaagcgatca tcgctgaatt ccaaagggaa cgagtaaaat tcagctcatt
     2941 ggaaaacaaa ttttgaaaat tgatgatcaa gttactgcca atctttattt ttcccgatgt
     3001 gatatccacg ggtgtattcg tcattcccaa aaccatccag acaaaaggga aaatggaaat
     3061 aaatgatagg atgcttaatg ctgtatactt taatactgac ccaattgtcg aacgaagttc
     3121 taatgcttgt tttttgacgt tggggcgttc gattgtttct ttttctacca tatttttcat
     3181 cgactttctc ctcctacttt cgtttggatt atcgacaata tcacaataaa ggaaacaata
     3241 acaaaggaaa ctgctgccgc atatccaaaa ttaggcgtga atttataact caaattatag
     3301 atgtattgag atagggtggt cgttgcattt gctggtcccc cgccagtaat attttgtact
     3361 tcatcaaaaa gctgcaatgt cccgatcgtc gaagtaatgg aagtaaacaa aatgattggt
     3421 tttaaatttg ggatcgtcac tttgataaac tgctgccatt tagaagcacc gtcaatagat
     3481 gctgcttcat aaatctccga atcaatattt tgcatgcctg aaaggaaaaa aatcatattg
     3541 tatccagtcc aacgccaagt gatcgaaaga atgatcagaa ctctcgccca aattggatga
     3601 gtcaaccagc caatcggtgt gtcaatcata ccaatgccca acaacaagtt gtttacaagg
     3661 ccagattgag aaaatagact tttcatgatc agagaataac tgaccatcga agtgatacat
     3721 ggaagaaaga tagcggttcg aaacaaccct ttatatttca actttttatc atttaaaagg
     3781 ttggaaataa aaagtgctag aaacaacatg atcggtactt gtacaagtaa atacaggaat
     3841 gtattaaaaa aagctttttt aaaagttgca tctgtcaaca tccgctgata attacctaac
     3901 ccgttaaatg tcatattgac agggggacca ctttgaaaag acgtgatcaa agccaaaacc
     3961 atcggaataa aaaccatgat tgatatcaaa atgactggca agattaaaaa agcatgcccg
     4021 tttttgaata aatctttctg cttcattctt ttccctccaa ttctatcatt gattatctac
     4081 atgaaaagac aagaaagaca atatctgtaa ggcagacgtg atcttttttg tcttttcgtt
     4141 tgtctgatga aaaagcgaag aaattcactt ttttaccacc gatcaattag ccaattgtgc
     4201 ttctacttgt ttttgtgtat ctgctagtac cttgtctgtc tcttctccat tgatgattcg
     4261 atgaagagct tcagctacga ctgcttcaat tgcataggtc tcatgtccgt aattcacttc
     4321 tggtatttca gatgtccatg ttgaaaaatc gtcaaagatc ttttgaccac tgtaaaattc
     4381 agaagcttct tgataagctt cttgttcatt cgcgctaagc atcgtagata ctagtccgat
     4441 ttctttcgca agagtgccca tcagttcttc attcgaagca aacgttgctt ctaggaaatc
     4501 tttcgctgaa tcttctccag ctactccttt gatcacatac cagccagcgc cgccaaggtt
     4561 agaagcttgc gctttttgca gattttctgg aagtgcaggt attggtgcaa tcttccattt
     4621 tccagattgg ttttctgctc cttggatcgt tgaagaatac caagcgccag ttgggctaga
     4681 agctactgct cctgattgta tcgcagttac tccagcattc cagtcagagg tttgttcgac
     4741 tagatcttct ttcaaaagag tggcaaacaa ttcaagacca tactttaaag atgcattgtc
     4801 ttggatcgtg actgtctccc catcttcaga tgtataccat tctcctgcct gttgcataat
     4861 catccttact cgaccaaggt cagaaggatt gacttcagcg attttctttc ccgtcttttc
     4921 tttgacatct cgcgctactt gaatatactc gtcccacttc agattctcca tatcctcttc
     4981 tgtataacct gcttctgcca tcagatccgt acgatagaag tttgctgtta cgcccgaatc
     5041 gaaaggaaca ccgtatattt tatctcctac tttgtttacc gcaaatttat acggtgcaaa
     5101 atgatcttcc tttacaatag cagacaaatt agcgaatgca tctggataag aagtcaaata
     5161 gccttgtatc ctataatctt caatcaagac gatattgggc aatccttctg tattcccgct
     5221 cgctaaagcg gtgttcagtt tctgaacgat atcatcctga gacatcgtaa caacttctat
     5281 ctctgtttct tcgttctcat aaactttttt tgcttcattc actgctttga tattgaatgt
     5341 ctcatcccac gcccagacag tgactttttc ttctttgtct gctgtactac tagcacttcc
     5401 gcatcctgtt aagaaacctg atgctgccgc taaacaaacg actccagcaa ttaactttct
     5461 gatgttcatt tttatccgct ccttttccat ccattagttg tttacgtttg ctacattcat
     5521 cataccaaaa tttgaaaacg gtttctttat tattcagata cttcaaaaat ttctcctaaa
     5581 ataataaaaa aatacaaatc aactatgaca aaaggaataa tcttagcatc tagaccaaag
     5641 atcgaaatgg tgaggtttct ttaaaataaa aatatccttg cattttctcc tagaaataag
     5701 tatttttaaa ttacaagact attcttaagg cgggtaaaaa aatgccaata cgcgtaattc
     5761 ttatcgatga tgaacagacg attttagatg gaatgaaaca gttgatcaat tggtctgagc
     5821 acgggtttca attaataggg actttttcca gtccgctgaa agcgcttgat ttttctttgg
     5881 tacatgcaat cgatatcgtg gtcacagatg taatgatgcc tgaattaaat ggaatcgaat
     5941 tatcgcggct attaaaacag caacaacctg aaacacagat ccttatcctc tctagctatg
     6001 atgaatttga tatggttaaa gattctttta aagaaggcgt cgctgattat cttttaaagc
     6061 ctcgattgag tcctgatttt tttctaaatt ccttaactgc cgtttcacaa aaaatcaaaa
     6121 aaaagcccaa caaaagccca atcaccaatc gttatgagca gatctgcgag aatctttcac
     6181 agcagattgc tggacaaact cgtccattgc ttgaccctct tctttttgaa cacaccttgt
     6241 ttttctgtta tataccgaaa aacggccatt cagttcaaaa caaggctcta aacaaaaaca
     6301 gtcgatcacc cattcattga tggatagcga cgattcgttt actatttatc ctgtttctac
     6361 cttttcggaa gaatccggct atgtaatcaa tactgattcc aaagatacac ttaggaagtt
     6421 tgttcaaaag tttcgtcaag aagtttttcc aggtgatttt tttgttgttt ctgattcttt
     6481 tgcaatccaa gatatcaatg aaatattcca agagatcaga aattgcacca aaggacaacg
     6541 tttctatcat aagggaaaga accttgctta tcagcatgaa cttcttccgt tattaaccaa
     6601 ccacgagcag caagcaagta gttatttaac aaaaatcgtt cataaagact acgctttagc
     6661 tgtcaaagaa cttgagaatt tcttcacgac gatattggaa cagcctattg atccagctta
     6721 tttgaaacat gaggcaatca atcgtttcta tgcattactc aacgctttgg ctgacgagca
     6781 tgttgggaat gaagaacttt ctttgctcaa gatcgccctt ccttctttat tagccgatgc
     6841 tgagtatttc gaagattttt ctattcttgt caaagaaaca cttgagaaac tcacccactt
     6901 ttctgcaaat gcaaaaaaag acaatttcga tatcttgact ttgatctacg agttcgtcca
     6961 ggaaaattat gagaaagaaa tcaatttaca acttttatcc gaaaaatatc atttttcata
     7021 tagctatctc tcagccatct ttactgaaaa atttggtatc aacttttcaa aatatctaaa
     7081 aaaagtccgt atcagtaaag ccaagctctt tttgacggaa acacggctta ctttgacgga
     7141 aatctgtgag aaagtcggct ataccgaact gggttatttc tccagagttt ttaaagaaga
     7201 gacaggaatg acaccttctc aataccgtaa aggaactttt gtaaaatgag aaaaaaaatc
     7261 aaagagcatg aactctttgc caaacttctg ttcattttgt tcatcggtct ttttctgcaa
     7321 gccatcgtca tttctttgtt tatttatcat cgatcaaggg atgcctatat ccagctgttc
     7381 aatcaatcga atgatgtggt actaaaaaag attcaatctg aatttgaaag tttgaatgat
     7441 acaatcgaga atacattagc cgcttttgat tctaatccag ctgtcaaaag ctatttttcc
     7501 aatgatccag cccaacatat ggaacagctt cagcaactaa gaacaatcca aaaaatgaat
     7561 gactctcttt caaaaatcca tccaatgatc gattacgatg tattgatctt tggagaaaat
     7621 ggaagaactt ttgtcggaaa cgacatgctc actgccgtat ctgccgattc tttttttcaa
     7681 tctgcgattg ctcagagagt gaatgaacgt gctgctgata cacaaatgct ttttgcacac
     7741 cacggattga ctcttcgcga taagaaagca cccagcgtct tctttgtcaa aaaactgaaa
     7801 aatacattga atcacgttta cggatatgct gttttatcga tctcatccaa gcaactagcg
     7861 aatctttttc aatctgtagt aaatcctgag atatcgaaga tctccttcat gaacgaagaa
     7921 cagacgatca tcgcatcaaa tgaagaaaat acaattggga gccgatcaat gattttcaaa
     7981 aaactaacag taggtgaaac aacgatttca aatggacagc agttgacccg tcttcctctg
     8041 taccgccagg attgtacatt actcagtcaa gtcgatcttc attctttatc taaccaaatg
     8101 ggcgtgattt ttccgattat tttgtataat atcgtgacac ttgcttttgc tggctcattg
     8161 gtatttatgt atttggatcg acacacaaaa tcgatctacc gcttgattga ttcgctaaaa
     8221 cggctagagg aagatggctt aaaagctact gttcctgtac aaggaagcca tgaagtccaa
     8281 ttgctaggga atacgatcaa tcaattgcta agtaatgctc agcatcatta tgaacagacg
     8341 atccaagacg aaaagaagaa acgttcgctt gaaatccaaa ctatgcaagc acagatacag
     8401 ccccatttta tttataatac gctgactagc ttgaaatttt tgatttggca gaaaaaaaca
     8461 gatgaagcag ttcaaggaat cgacagtttc attactttac taagaagtac gattgggaaa
     8521 aaagaagaag tgattccagt caaggaagaa atcaaaagcg tacaaagtta catcgatctt
     8581 ttgagtatgc gatatggcga aagaatctcg gctaaaataa tgattcctga tgaactgcag
     8641 tcattatcta tgccgaatat gatccttcaa ccaattatcg aaaacgccta tcttcatgct
     8701 tttcacaata aaacgcaagg attcatcacg gtgtacggca aaacaaaaaa tcagcttctt
     8761 ttattcgaag tgattgataa cggcgatggc ttcgatactg aaaataccaa acaaaaaaat
     8821 gatcattttt caggaatcgg catcagccat gtagatgagc ggatacgcct aatgtatggc
     8881 gatgcatatg gcttaacgat ccaatcagtg attgggacag gaacgactgt aacgatttgt
     8941 ctaccgcttc ttcctaaaaa tcaatcagaa tatcaaaaat aaagaatagc caagcgctga
     9001 tttttttatt ttaagttaaa taaatcaatc agtattcttg actttcatct acgcgtctct
     9061 tctactgtca gcactggata acagcccaat atttgacttt ctcctccgag catcttgatt
     9121 tcttggagcg cattctctac taatgtcatt ggacggtcca acaacaaatc gatcacgaaa
     9181 aaatattctc ccaagctagt tttcaatggt cgggattcaa tcttacttaa gttgatttcc
     9241 cgccagccaa aagctgccag cattttatga agcatccctg gccgattagc aggtagtgtc
     9301 aagatgacac tcatttttac tggagctgac tgctggctgg tcatctttcg gtcaccgacg
     9361 atccaaaaac gtgtctggtt caactcatta tcttggatat tctcagctaa gatctccaat
     9421 ccaacatgct gtgctgtttc taatgaagca atcgctgctg cgtcttcttt cggatgttct
     9481 gctacataca ttgctgcagc agttgttgat tctgttgcta ctaaaggaac attaggataa
     9541 tgcgtttcta aaaactgttg tgattgtgct agtgcttgtg gatgtgaaag gatttttgta
     9601 atctttgttg ctggattgcc taaaagctgc tgcttgatag gaagaacgat ttcactttta
     9661 acttcgactt ctggatgttt ggaaagaaga tcgattgtgt gatgaacagc tccttccaat
     9721 gaattctcta caggaacgac agccagatcg acttgtttgc gaaacaaagc gcgcaaacaa
     9781 acagggatgg aagcatacgc agtcaattgt tctgtaggaa atagctgaca agccgcctga
     9841 taagtgaatg aactttctgg ccctagatag ctgacgttca tatgcttttg atcctttcta
     9901 taatggtatc gatgatcttt tctggtgttt gattggtcgt gttgattcga tgtgtcgcac
     9961 tttcttcata ccaagaaagt cgttgtgcca ataatgcttt tatttcttct cttgtttttc
    10021 caagagctaa aggtcgaata gacgtttcat cttgttggat acgatcaact agacagtccg
    10081 cttctgcctc taagtaaaga acaatcggct gtgtttttaa aaaacgccga ttttctgcac
    10141 tttggacaat ccctccacca gtcgcgataa ttttgtcttc atttgacagc tttcttaaaa
    10201 tatctgactc cacttttcga aagctttttt caccgtaata gtgaaaatat tcggcaatcg
    10261 acaattggat atgttcctca atctttgtat ctaaatctac atactctttt tgtaaacatt
    10321 gagctaaccc tttaccgatt gttgtttttc cagcgcccat gaaaccaatc agcaaaatgc
    10381 tagccatgag cttctcctcc tgacacaagt tctgctaaat cgtcaaaaaa tgccggataa
    10441 gagattgaaa cagcttcagc tccttctagt tcgcaaggtc catcagctat caaagcagct
    10501 acttgcagca tcataccgat tcgatgatcg cctctgctgg acacttttgc gccatgcaga
    10561 ggtgtcggcc catggatgat cagaccgtct gctgtcgctt ttatctttgc tcccattttc
    10621 tgtaattctt ctgccaccgc atcaattctg ttggtttctt ttacttttaa ttcttctgcg
    10681 tctttgatga tcgtgattcc ttctgcttga gtagccacta aagctagaat gggcaattca
    10741 tcaatcagac gcggaatgat ttctccacca atcgttgctt tcttcaaatg actcgtcttg
    10801 acacttaaat cagccgattg gttctgttca ttgtgattcg tctgggtaat cgctgcgccc
    10861 atctcctcca acacatctaa gataccagtt cttgttggat tgaccccgac attttttaac
    10921 aacagttggc tttccggaac aagaagtcct gctgctagaa agaaagcagc tgatgaaata
    10981 tcacctggaa cagttatctg ttgaccagtt aatttttgcg gtccggtcac tatgatcgtt
    11041 ttatcttcca ctgtgatcct tccgccaaat tgacgaatca tttcttccgt atggttacgg
    11101 gaacgttctt tttcaacgat tacggatgtg ccttctgctt gtaatgccgc aaacagcaaa
    11161 gcagatttga cttgcgcact ggctacaggc atattgtatc ggataggtga taaagagcgg
    11221 gttcctgtca cagtgattgg tagatactgg tcattttctt ctccttgcaa gtcagcgccc
    11281 atctcgcgta aaggttccat cactcgcccc attggacgtt tagacaacga agcatccccg
    11341 aacagagtag tcgtaaaagg ctgtccagcc aaaataccca tcaataaacg tatggtcgta
    11401 ccggagtttc ccatgtcaat tgattcggtt ggttggacaa atgagtggct accgcgacca
    11461 tgaatcttga tcacctcttc ctcttcttcg atctcgacac ctaattgttg gaaggcatgt
    11521 aaagtactca gacaatcttc tgcgcgcaaa aaattttgta tagtcgtcgt tccttcagca
    11581 attgctccaa acatgatgct gcgatgggaa attgatttat ctgctggtat cctaacagtc
    11641 cctcttaatc catgtatttg ttgtaataat tgcatttatc cagcctccta tttgatccag
    11701 caatgataac tggtattaga cataatgatt ctttttgctt gtaacaaatc ctgttggttt
    11761 ttaaaactga tttgcagtat tcctaaaata tcttccctgg tctcttggat cttcaaatta
    11821 atcaaagaaa ctcccgcttt tccgatcagt ccagttactt ctgcaataac gccaggttca
    11881 tctggcacat cgataaataa atcaaaaaag gcaggaatcg cgccttgctt atggacaggc
    11941 aactggtctc gactttcttt tgcccgattg aaaaaagaaa agatttccga ttggttctca
    12001 gtcatgatcc attctgctat ttgtgtcatt tcttgttgcc aatctgccaa tcgttccaat
    12061 aatatctgct tattgctcaa caaaatatct gtccacatga caggatctga ggaagcgatc
    12121 ctagtgatat ctcgaaagcc tcctgctgcc agttgtttgg ctcgcggata ctcttgattg
    12181 aattgttctg cctgattgac taagccagct gccagaatat gtggaaaatg acttagcatt
    12241 cctgttatac ggtcatgttc tcttgctgta agagttacat attttgcttt ggttccgcaa
    12301 aatagttctt gcagtttttc aacatctttt ttcgtctttt ccaaaggtgt aaagatgtaa
    12361 taggcatttt caaatagttt agcatctgct gcctgtatac ctgatttatg agaaccagcc
    12421 attggatgtc caccgacaaa ccgaagattt ttctgctcag cggatgtcac gacttgctgc
    12481 ttcgtactgc tgacatccgt aatgatgacg tgatcagaca agttgattct ttcaaggaca
    12541 tcaagataag ctaaggttgt acggacggga acggctaata agataacgtc tgcttgttct
    12601 gcacctgttt caaaatcact tgcaacgtaa tcaacgatct gctgttctac tgcttgctca
    12661 cgggtttttg actgactatc ccaaccaact actatcagtt ctggattggt ctgacgaata
    12721 caaagtgcca tagaagcacc aatcagtcca agccccacga taaaaacatg ttgtgccatc
    12781 ttatttgccc cctttttagt aatgtttcaa gtagtcacgg tactccttca ctgcagcttt
    12841 caactcaaca agcgaatcac ttgggaattt tgacaatagc tctgtcgcta ccacagtggc
    12901 tacaacattt tccgcaacga cgctagctgc aggaactgct gtactatccg aacgttctac
    12961 acttgctttg taaggttctt ttgtatcgat atcaacactt tgaagtggct tgtacaatgt
    13021 aggaatgggt ttcatgaccc cgcgaatgac aagtggttcg ccattggtca ttccgccttc
    13081 gaagccgcct agattattgg tacgtcggaa atagcctctt tcttcttccc ataggatctc
    13141 atccatcact tctgagcctg gtaatgaacc agaagtgaat ccgaccccaa attctacgcc
    13201 tttaaaggca ttgatactaa ccacagcttg tgcgattttg gcatctagct tcgtgtccca
    13261 ttgtacgtag cttcctaatc cagctggaac gcctccaacg agcacttcta caattcctcc
    13321 gatcgtatct ccatttttct ttgtctgatc gatcaaatct tttattcttt gctcaactgc
    13381 tggatcaaca actcgtactt ctgacgcttc agaaatttct ttgatttctt gaatcgagat
    13441 atttccaggt atttctgctt ggattccacc aagacaaatc acgtgtcctg ctacgatgat
    13501 gtcaagttct tccagaattt tcttggcaat tgcgccgatt gctacacgca tggtcgtctc
    13561 tcttgcagag gaccgttcaa gcacattccg aagatctcga tgatggtatt tcataccgcc
    13621 taccaaatcc gcgtgtcctg gacgcggacg tgcaactcgc cgcagtgatt ggtctttttc
    13681 aggcacttct gctacagaca tgacagttgt ccaatttttc caatctttat tttctaccac
    13741 taaagtgaca ggtgaaccaa ttgttttgcc gtgacggatg ccagacgtga tccgaacacg
    13801 atctttttcg atcttcatcc ttcctcctct gccgtaacca gcttgtctgc gagcgagttc
    13861 gtgattgatt tcttcttctg acagaggtaa tcctgctggt agtccttcaa taatggctgt
    13921 taattctggt ccatgggatt ctcccgcagt catataacgc atcttacagt cctcctaagt
    13981 aatctttgaa tgtctctgtg ggaacaggaa cgatttttgc ttgcccaatc ttttctagca
    14041 agatgatatt caattggctt cctcttgctt tcttatcatg ggttatcgcg tggaataatt
    14101 catctggatg acttagatcg tacgtaatcg gcagatggaa tttttgaagt atcctttcta
    14161 aagcagctgt cgatccagtt ggagacaagc cgattttttc tgcatgagaa gtgatggcta
    14221 ccattccaat agctactcct tctccatgac tgactacccc atatcctgct gtattttcaa
    14281 ttgcatggcc aattgtatga ccaaaattta aaataagtcg atttccttgg tcaaagacat
    14341 cttcttccac tacttttcgt ttgatttcca acgtagcagt aatgattgga acagcatgtt
    14401 ccagaagatc tttttcgtct ttcagctcgt tcagctgttt ccaaagcgtt gcatctgcta
    14461 tagctgctga tttgatgatt tcagcgattc cttctcgtaa acgacgtacc ggcaaagttt
    14521 ttagaacctc aggatcaatc aatacaccag ctggctggct aaatgttccg acaaggtttt
    14581 tcgctttgtt tgtattgact gctgtcttcc cgccaatact agaatctact tgtgcaagta
    14641 gcgtagtcgg aatttgaacg aaatggatcc cacgcatgaa cgtcgaggcc acgaatgctg
    14701 ctagatcacc caccattcct ccaccaagtg caagtatccc atcgctgcgg gtaaaatcat
    14761 tatctgctaa aaaatcgtat aactcagctg cttgttctaa agatttactg gtctcacccg
    14821 ccggtacgat tgaaaggact ggttcaaaat tagcttgtct caattggaac atgatttttt
    14881 ctgcatatag tggagcaacg tttgtatcag tgatcacagc tactttttga ggtctccaaa
    14941 tcgtttgaag ccatgatcca gcctgttcta gtgcgccttt ttcaatatga atatcgtatt
    15001 tttggttatc aacatgtaca gtaagcattc gtttcacttc tttcttttaa aaagctggaa
    15061 ttaatcgagt gtctgaatct tttccattgc ttctttcatg atcgcaactt ctttcatcat
    15121 gcgtgagtaa gtcttttcat tcaacgattg gggaccgtct gaccaagcat ttgcaggatc
    15181 aggatggatc tctacgatca tgccatctgc tccgcttgcc acacccgcac gagccattgg
    15241 tggtacaagt tcccaaattc ctgtaccatg acttggatca acaatcacag ggaaatgagt
    15301 taattttttc atcaatggga cagcacttaa atcaaacgta ttacgtgtcg ctgtttcgta
    15361 agtccgaatc ccacgttcga taaaaatgac aggagatttg tcttcgactg caatgtattc
    15421 tgcagcatta agccattcat tgatcgtacc ggaaattcca cgtttcaatc caacaggttt
    15481 acctgtttta ccgacagcag atagcagttt aaaattttgc atattacgtg cgccaatctg
    15541 taggatatcg ctgtactcag ccaccatatc gatatgccct tcatccatca cttcagtaat
    15601 cactttcatt ccaaattcat cagccgcttg acggatgtat ttcaatcctt cttcttctaa
    15661 accttggaaa gcatatggag aagtacgtgg tttgaaggca ccacctcgca gaatttttgc
    15721 gcccccggct ttggccattc gcgcacattc tctgatttgg tctaatcctt cgatagaaca
    15781 aggaccagcc atcgtcacga aactgccgtc accaattttt actccgtcta catccacgac
    15841 tgtattgcca ggatggaatt ctcgagaagt caatttgtat gtgtttaaaa tacgcacagc
    15901 attttctact ccttcatagc gtgtaaatgc tccttcttgt aaacttcgcg tatcaccttt
    15961 tagtccaatc actactcgtt ctgttccttg atcgattgag actttgaatc cttctttttc
    16021 gatatgatcg acgacttttt ttatttgttc ttccgttgct tcttttttca taattactat
    16081 cattgttttt cccgctttct tttgtttact tatctatctt ggctgatttt ctctactttt
    16141 tcaaggtggt tttacattaa ttcctactaa atgaccgtgt cctgttgttc tcattttcgc
    16201 tccttctttc ctacttatga cccagataat caacaaaaaa agatgtccct gtaggaaatt
    16261 gcctctacag tgaacatctt caacgagcct taaaaagcgc tgcaaaaata taaaatagcc
    16321 actatagatg aatttctcta cgtggctgta agtcgtgttt aaaaatcttt agccatcata
    16381 gatacaaacc tggtttgttt gtacccacga agtcatgaat acaaacctat ctaaaatagc
    16441 taaagtaata cgtattcagt tttgtaagtt ttctagattt cataaaaaat cgctccaaac
    16501 tgatgtaaga attgtaccta gtatagacat aaaaaaatcg actgtcaatc atttttatga
    16561 aacttttttt aattttctga tagttaactt tgtttttcag aaaaataaca attggtttta
    16621 ttgaagaaaa tagtcatgaa caaaagtatc tgagaacttt tttactctct tgttcatgac
    16681 atatagaata aagcagcttt gtcttgtaaa ctccgttttt caaataataa tctaaagatt
    16741 gctactgaat aataaagccg atttcatggt cctcttcgct gctgattcgg taaggtgact
    16801 caacatccgc tccccagcta ttgatacccc cgactcctct tacttttgca aaaatcgtaa
    16861 gaaccgttcg tctttctggc ggtagttctt ctatatgtgt tgcctgttcc agttcttctg
    16921 ctttgaatgg taaagcggaa aaagcaaatg gcgtatcctg ctgttcaaat cgcaacgaaa
    16981 aatcttcttg atctttatcc gtattatcca acgtttgatt tcttgttagt tctacccatt
    17041 tcgtttccat atgcatgccg cattcttggg gaacaaggta tggggaaacg gggagtcctg
    17101 ccactttata aataccttcg acaccaccag ccatccgatc cggatatgtt tcccctgata
    17161 ggccttcata ctcaaaaaat tttgctttcg ttggcataat gaaccttaaa cccaatgcgg
    17221 gtaattccgg taatcctttt tgtccataat aatgcatatt gacaagcatt ttaccctctc
    17281 cagcgatttg gtaggaaatt gtcacttggg tttttggtac tgtgttgatt tccagtgtaa
    17341 aaatgatttc aaccgtttca gcgtattcat tacttgaata ttgattattt actggagcaa
    17401 tcggcaaatc gatcttttta ttgtccacca gtacttctat ttctttgact tttggataca
    17461 atccggctcc taaccacata ccggatttaa atccaaatcc gctacctctg tcattatcgg
    17521 tcgttgctcg ccaaaaaact ggaagtgttt cccggtatag ccattctttt ccgtttttat
    17581 tgagcgattc cagtccgcct cttgtataag aaaaaatata atgcgtattc cccgatttga
    17641 cacctaatgt acagtcgccg aagacgattg caactttttc cgtatccata tgacttttat
    17701 ttactgtatt tttcataata atatcttacc tcctgcatgg cgggtttttc ttgtccatct
    17761 gcaaaaacga tcccattccc tgagaattca tagtcacttg gtttatcgtc aaagtcaccg
    17821 ccatagcgca tcacttctct gcctgtgatt ggatcagtca cttggatcgc ctgatcttta
    17881 aaatcccata tatagcctcc ttggtacatt tcataacgat caagcaacgt catgtaagaa
    17941 tccattccac ctaacgaatt ccccatgtca tgcatatatt cacataagat gaacggtttt
    18001 tccgctttat tttcaaggta gtcaatgatc tcttctggtg gcgcatacat gcagcttttt
    18061 acatctgaaa tcgaatcttc gaatgctgga tttggaaaat tcccttcata atggacaagt
    18121 cgatcggggt ctactttttt gaagtaatcg ttcatttgtc gaatgttttc ttccgcataa
    18181 gattcatttc caagagacca aaaaagaata cttggatggt tttttaatgt ttcgaaattc
    18241 gtttttgctc gatcaagaac agccgcctgc cactgaggta ctgcacctgg tacgttgtaa
    18301 gaaggctcaa atgcgcccat tttttgccat gagccatggg attcgaggtt cgtttccgcc
    18361 atcacatgga ttcctgcttg gtcgcataaa taataccaag tgatctggtt ggggtaatga
    18421 gatgtccgta cgccgttgat ctttgcattt tttatgatag ccaaatcttt ttccatatct
    18481 tctatagtga tggcgcgtcc ttttttcgga tgccactcat gtcgatttac cccgttgata
    18541 atcatccgtt ttccattgaa gtagatgatt ttatctttga tttcgatttt acgaaaaccg
    18601 aaggaatagt caatatactc cgttactctt ttttcttcgt ctatcgtctc caattcaagc
    18661 ttatataaat tgggctgaaa atgcccccac agtgccacat tttcaaatcc atcgatgcga
    18721 atcgtcattt gctcttttaa cggatattgc tgttgataga tttccttccc atcaggatct
    18781 agtatcgcta cattcagctg actgtttttg gctacttgct caaaagacaa atctacttca
    18841 agtttgccat tttgacattt gtcatctaag cgaggtttga tccaaagatc ttctaaatgg
    18901 ttttgtcctc gagcaactaa gaagacatct ctgaagatcc caaaaaatcg gaaaaagtcc
    18961 tgatcttcca agaatgcagc tgtacttctt ttgtaaacct ccacagctag aatattttct
    19021 tcttccaaat aaggtgttaa atcaaattca gacggagtga acgaatcttc ggcatacccg
    19081 ataaaatgac cattcaacca aataaacatt gcttgttcta caccagcaaa atgaatcgag
    19141 atttgttttc cctttagacc ttcttctaat cgaaaagtcg tcaaatacga accaaccgga
    19201 ttgtctttag cagctgaaaa gatcccttcc gcctctttct cagggcttgt cgtgaaaggg
    19261 ggacggcgaa atacatgtcc ctcccacgga tacatcgtat tgatgtaatg gatcttgtca
    19321 taatttgcta attcaatatg actaggaaca gtgatttcat caaaagaaca ggcatcaaaa
    19381 tcttttttat aaaatgtttt tggtcgtacc gaaggatttg gcgaataaca aaaacgccat
    19441 gatccattta aagactgttt aagggaggat gtggtttcta cttcttccct tgttgcataa
    19501 caaacatgat cgctatgtgc atttgattgg ttcactctaa aaacttgtgg atcatctaac
    19561 cattttatat ttgctttcat acttttttcc ttctctctca taactttttg aaatgaagca
    19621 gttctgtttg gaaatcacct tgaaaatcgg ggaagtaaag tccaactgtc atcaattcgg
    19681 cccccgaaaa tatccgcttc gaggtctgtt cctgataaag tgcctcatca tctaacccca
    19741 tcagatatac ttcatggaac gctggttgag ctgaagctag tatcctccct aaaaacacaa
    19801 tagcttcatc ttgtttagga gacacaaaca tccatgcggc aatattttct tcaaaaggac
    19861 tcgccaaacg ataataatgg ccatactgga tcaccggacg aatcgtttga taggatataa
    19921 tttggtcttt tacttgcttt ttttcttcag gagacagctt tgtcaaatcc agttcataac
    19981 caaaaacagc gctcatagct gtttctcctc ttgttttcaa cgaagtagat ctgtcggtct
    20041 gatgatttgg tacagctgat acatgagctg tgatggacga aatcggataa gcaaggcttg
    20101 ttccatattg gattttcata cgctccactg catccgtgtt atcacttgtc catgactgcg
    20161 gcatataata aataaacccg gcatcaaacc gtccacctcc accagaacag ccttcccata
    20221 agatttccgg ataatcagtt gtcaaatgtt caagtagttc ataaagcccg agcatataac
    20281 gatgcgctac ttctccttgt ctctgtgggt caagggtaat cgaatagaca tctgaaagcg
    20341 aacggttcat atcccatttg atgtagtcaa gaggaatcgt atctaaaatc tttctcattt
    20401 gtttctcaat cgtctgccga acatctaacc ttgtaaaatc cagtacatgt tgtgatctgg
    20461 aagcagaagg cattctacct ggctcctgca taagaaaatc aggatgtgtt cgatacagtt
    20521 cagaatcaac cgagatcatt tcaggttcaa accataaacc aaatttcaat cctttgctat
    20581 gagcataatc tgctatttca cgtaatccat ttgtcaactt gccttcatac tcgaaccagt
    20641 ctcctaatga agaattgtcc gcatctcttt ttccaaacca gccatcatct aatacgaaca
    20701 tttcgatgcc tagctccttt gcttcatcaa tgatcgcctt gattttttca ctattgaagt
    20761 caaagtaagt ggcttcccag ttattgacca gaatcggccg ttcagcaaat tgatgacgac
    20821 ctcttgcgat tctttcacgt agcaactcat ggtggatctg actcattttg ttcaatccct
    20881 gacttgagta agaaaggatc atttcaggtg tttgaaaaga ctggcctgca ttcaattccc
    20941 acgaaaaacg atggctgttg atcccaccga cgactctcaa ttgattgatc tgatcttttt
    21001 caagttcgaa agaatggttg ccggaataaa caaattgaag tccgatcgct tctccagtat
    21061 tttctgtcgt atgatgatga actaaagcaa taaagttatt catatggtga ctgctcgttc
    21121 ctcgacggct ttcaaatact tttgttccag ataaaacaga ttgccgtgaa atctgtcttt
    21181 cattgacatg tgctcctggc aaagcaatga cttcatcaaa tcgcctagta tgggcaaagt
    21241 ctagctggaa actagccgct ttttctagtt tgattgtttc tccggtttca tttcttatct
    21301 ttacagatcg tgtgatcact gctctatcag taaatatggt atagaacaaa tcgaaataaa
    21361 tcgctgtttc tctatcctta agtgaaatca ccaacgtttg tgcctctgac ttgtccatca
    21421 cataagtttg aggcaagcct tcgatctctg gcttcccatc tgtaatataa tggctgtcat
    21481 atctcagatc cagtaagtct gatccattct tgcgctgaat gatcgatgcg ggcgttcgaa
    21541 agtccatgct gccatgagaa gagtattctt gcggtagtgt atctttcgac agagaccggt
    21601 caggatttaa gggaacattt cctgaaaatc cacgatctct tcggggataa tttttattgt
    21661 ctttgtactg cttgactggt ttgccaaaat aaatatgcgc caatacattg ctttcttcta
    21721 tctttatcag ataagaaaga aatgtatttc gtaaatggaa gactcggctc ttttcatcaa
    21781 ataagatgaa atttttatct gtttttctat gcgtaatatc catcatttca ctcccaacaa
    21841 aacggaaacc gtttactttt ttt
//
LOCUS       DAHUVV010000040        21811 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.040, whole genome shotgun sequence.
ACCESSION   DAHUVV010000040 DAHUVV010000000
VERSION     DAHUVV010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.040"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            88..1092
                     /locus_tag="NK767_001505"
     CDS             88..1092
                     /locus_tag="NK767_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6694505.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1093..2037
                     /locus_tag="NK767_001506"
     CDS             1093..2037
                     /locus_tag="NK767_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6694506.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2037..2999
                     /locus_tag="NK767_001507"
     CDS             2037..2999
                     /locus_tag="NK767_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6694507.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3026..3940
                     /locus_tag="NK767_001508"
     CDS             3026..3940
                     /locus_tag="NK767_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6694508.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3966..5747
                     /locus_tag="NK767_001509"
     CDS             3966..5747
                     /locus_tag="NK767_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCH6694509.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6095..6781
                     /locus_tag="NK767_001510"
     CDS             6095..6781
                     /locus_tag="NK767_001510"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCH6694510.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6801..10382
                     /gene="smc"
                     /locus_tag="NK767_001511"
     CDS             6801..10382
                     /gene="smc"
                     /locus_tag="NK767_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCH6694511.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10391..11200
                     /locus_tag="NK767_001512"
     CDS             10391..11200
                     /locus_tag="NK767_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCH6694512.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11211..12209
                     /gene="ftsY"
                     /locus_tag="NK767_001513"
     CDS             11211..12209
                     /gene="ftsY"
                     /locus_tag="NK767_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCH6694513.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12396..14663
                     /gene="gshAB"
                     /locus_tag="NK767_001514"
     CDS             12396..14663
                     /gene="gshAB"
                     /locus_tag="NK767_001514"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HCH6694514.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14752..15204)
                     /locus_tag="NK767_001515"
     CDS             complement(14752..15204)
                     /locus_tag="NK767_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HCH6694515.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15354..16304
                     /locus_tag="NK767_001516"
     CDS             15354..16304
                     /locus_tag="NK767_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCH6694516.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16297..17256
                     /locus_tag="NK767_001517"
     CDS             16297..17256
                     /locus_tag="NK767_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCH6694517.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17253..18008
                     /locus_tag="NK767_001518"
     CDS             17253..18008
                     /locus_tag="NK767_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6694518.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18031..18984
                     /locus_tag="NK767_001519"
     CDS             18031..18984
                     /locus_tag="NK767_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCH6694519.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19628..19924)
                     /locus_tag="NK767_001520"
     CDS             complement(19628..19924)
                     /locus_tag="NK767_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HCH6694520.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20279..20635
                     /locus_tag="NK767_001521"
     CDS             20279..20635
                     /locus_tag="NK767_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCH6694521.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20645..21544
                     /locus_tag="NK767_001522"
     CDS             20645..21544
                     /locus_tag="NK767_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCH6694522.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         7202 a         3854 c         4652 g         6103 t
ORIGIN      
        1 aacctttgtt ataaaacgat ttattctgtg taatgtcctt ttttattaag aaaaacttcc
       61 atttaggaag cagagagggg tattcgattg tccgataacc aattattaga agttcagaac
      121 ctacatacag gattccgact caaagatgac tattacgatg cagtagatga cgtttcattt
      181 actttagaca aaaacgagat tttagcaatc gttggggaat ctgggtgcgg gaaaagtacc
      241 ttggctacta cgattatggg attacttgat cctaataaca caaaaattac aggtgagatt
      301 ttatataacg atctgaattt aatcgacctg aacgaaacgc tctataataa aatccgcgga
      361 aatgatatcg gaatgatttt ccaagatccg ttgtctgcat taaatcctct catgagaatc
      421 gaagaccaga tcaaagaagg actttcctat cacacaaaga tgaatgcaga acagcgtcaa
      481 gctcgtgcac tagaattgct agaacaagta gggattccta atcctgcgag agttggacgc
      541 caatatcccc atgaactatc aggcggtatg agacaacgtg tcatcatcgc aatcgcaatc
      601 gcctgcaaac ctccaatcgt cattgcagat gaaccgacga cagccctaga tgtaaccatc
      661 caagcacaga ttcttgattt actgaaagac cttcaggaag aaacaaaaac agggatcatc
      721 ttgatcactc atgacttagg agtcgtagca gaaatggctg atcgagtggc agtgatgtat
      781 ggcggacagt tcgttgaagt agcaggtgta aaagaactgt ttgatcatcc taagcatcct
      841 tatacacgct ctttactgaa ctctatacca caagaaggaa atcatgatgc agaactccat
      901 gtgatcgaag gtgtcgttcc ttctctgaaa aacatgcctc gtaaaggttg tcggtttgca
      961 ccacgtattc catggatacc agcaagcgat catgaagaag agcctatcct tcatgaagtc
     1021 gcaccgaacc acttggtacg ttgctcctgc tataagcatt tccactttag agacgaaaaa
     1081 ggagaggtgt agatgacaga attaatcaca atcaaagacc tgaaagttca ttaccctatt
     1141 cgcagtggat tcttcaatcg agtaacggat catgtatatg ctgtagatgg tgtggatttt
     1201 attatcgaaa aaggaaagac atatggttta gtgggagagt ctggctctgg aaaatctact
     1261 acgggaaaag ccgttgtggg attagaaaag gtaacagccg gacaaatcat gtatgaagga
     1321 aaagatgtaa cgaagcgaag caaccgcaaa aaaatgggtt ataacaagga tgtgcagatg
     1381 atcttccaag attcaatgtc cagtttgaat cctaaaaagc gagtgttgga tatcattgct
     1441 gaacccatcc gaaattttga acggttaagc gatcaagaag aaaagaaaaa ggtaaaagga
     1501 ctgctagata tcgttgggat gccggaagat gcgctatata aatatcctca tgaattttca
     1561 ggcggacaac gtcaacgtct aggagtagcc cgtgctgttg ctaccaatcc aaaactgatc
     1621 gtagccgatg aacctgtatc tgctctggat ctatctgttc aagcgcaagt attgaatttc
     1681 atgaaacgaa tccagcaaga atacggcctc agctatttat tcatttcaca tgatttaggt
     1741 gtagtaaaac acatgtgcga caatatcgca attatgtaca aaggtcgatt tgtagaaatc
     1801 ggtacaagag aagacattta taatgacccg cgacatatct atacgaaacg tctgctttca
     1861 gctattccgc aaatcgatgt agaaaatagg gaaagccaca aagaaaatcg acgaagagta
     1921 gaacaagagt atatccaaaa ccaaaaagaa tactatgatg cgactggtcg agtctacgat
     1981 ttgcgtacga tcacaactac acacaaagtg gcgttgaaag atggaggtgc aagctaatgt
     2041 ggaaaacgat tctgcgacgt gttttattga tgatcccgca agttattatt ttaagtgttc
     2101 tgatcttctt attagcccaa gcgatgccgg gggatccatt taccggtttg atcaatccaa
     2161 atcaggatcc ggcagtgatc gaacagatgc gacaagcagc tggcttgaat gacccatggt
     2221 atgtccagta cttccgttgg atcggaaatg ctttgcaagg cgattttggt caaagtttct
     2281 tatacaaacg ctctgttgct gacttgatcg gcgaacggat catcaacact ttatatctat
     2341 cgatcttgac agttgtcctt acttatttga ttgctgtgcc tctggggatg cttgctggcc
     2401 ggtatcaaaa ttcaatcttg gataagattg tcgtaatcta caacttcttt agttttgctg
     2461 ttcctttatt tatcttcggc ttgatcatgc tatttgtttt tggttatcgt ttaggctggt
     2521 tcccaacaag tggatcacaa acactaggtt tgcaaggtgg atttatcact caatggatag
     2581 accgtttgaa atatattctg ttaccatcaa ttacccaagc atttttggcg actgccgtca
     2641 caatccaata tctgcgaagt gaagtaatcg attcgaaatc gctcgatttt gtcagaacag
     2701 cccgttccaa aggggtaccg acgaataaag tattcagtcg acatattttc cgaaatgcag
     2761 cgttgccgat ggcttctcaa atggggtatg aaatcacttc gttgattgct ggttctgtag
     2821 ttatcgaaaa gatttttggt tatccgggag tcggaaaact atttattgat tccatcggcc
     2881 aacgggacta taccgtgatc acatccttag tattgatttt aggaatcgcg acgctagtgg
     2941 ggacattact ttcagatatc atcatgagta tcgttgaccc gcgtattcgg attcaataaa
     3001 accaatcaga aaggagaatg gatcgatgag tgaaaaaaac aatacagcag ttcaagaaag
     3061 tattcctcct atggggatac gaatgattgc tcgagaattt aaaaaagata aaattgctat
     3121 tttttcatta ggtttgctag tggttttatt attagtgatc tttatcggtg cgatgctgac
     3181 cgatcaagac aaagtgatgt atgttagtat cttcgacaaa tacgctgcac caggaacagt
     3241 ttcgacacaa ggacaaaagt tcattctggg agcagatgaa ggcggacgtg atgtattagg
     3301 acaattgatc ataggtgcta gaaactctgt tacgatcgga tttgcgatca cgatccttac
     3361 ttcgatcatc ggggttggat tagggatcat ctctggctac tacggaggga tcattgacaa
     3421 catcttgatg cggatcgttg attttatcat gattctaccg atcatgctga tcattatcgt
     3481 cttcgtatcg attatttcca gatacaatgt ctggtccttt atattcatca tgagtgcatt
     3541 ttattgggta gctaaagccc gattattccg aagcaagaca ttatctgaag caagaagaga
     3601 ttatgtcagt gcctcaaaaa caatgggaac caatgatttt aaaatcatgt tccgcgaaat
     3661 catgccgaat ctaagttcat taatcattac taatttgacg atgaactttg cggcaaacat
     3721 cggtatcgag acaactttga ctttcttagg tttcggcttg ccggcaaatg tacctagttt
     3781 aggtacattg atcggttatg caagtaacgg agaagtctta gtgaatcggc aatggatctg
     3841 gttacccgca tcaatcctta ttttagtttt gatgttgagt atcaattatg ttggacaagc
     3901 attcaagcgt tctgcagatg cgcgccaacg gttaggctag tatagaagcg aagggggaaa
     3961 taggaatgaa gaaaacgatt tttggatttg cagcgctgat gtctgctgtc gttcttgcag
     4021 catgcggggg cggaggtaca gatacctctg gaaacaactc tggaggcaac ggcaataaca
     4081 aagaggaatt aacttttcca attgcaacaa aaaataatga tgatgcaatc gaagacggcg
     4141 aattagaagc tgcggtcgca acggacagtc agtttaaagg attgttccag tgggagtttt
     4201 accaagattc atatgatgca gcttttatgg ctccttctca tgaaccgcta tttgcaaacg
     4261 atgacgattt cagaatcaca gacgacggag ctgcaacgct tgatctagat gaagatacga
     4321 aaaaagcgac aatcaaagta aaagacaacg tcaaatggtc agatggtgaa gatgtaacgg
     4381 cggacgatgt gattttccct tacgaaatca ttgggaataa agattacact ggtatccgtt
     4441 atgacgacac tttccgtaac atcgtaggta tggaagaata tcatgatgga agtgccgaca
     4501 caatctccgg aatcaaaaaa atagacgaca aaacagttga aatcgaatat aaagaagtaa
     4561 acccaagcat gcttcaatcg ggtggtggta tctggtcata tgcagcacca aaacatacgt
     4621 tagaaggtgt agcaatcaaa gatatggaat caagcgatca agtacgtaaa aatccagtga
     4681 cttttggccc ttattacatg agtaatatcg ttgcaggaga atcagtagaa tacttgccaa
     4741 acgagcacta ctggaatggt acgcctaaat tgaaaaagat cacgatgaaa tctgtaccga
     4801 tggcaagtgc aactgaagca ttgaactcta aattatatga tatgattttc caaatgccaa
     4861 cagatactta cgatacctat aaagaaattg ctggctatac aaacttaggc cgtgaacaaa
     4921 cttcttatac ttatctagga ttcaagttag gtaaatggaa tgcagataca aagagtgtgg
     4981 aatacaatcc agattcaaaa atggctgata aatcattacg tcaagcaatg ggctatgcat
     5041 tagataataa tgcagtaggt gaacgtttct atgcaggttt gagaagcaat gcgacttcat
     5101 tgattcctcc agtattccag tcataccatg atgatgagat gaaaggattc acacaagatc
     5161 tagacaaagc gaaaaaacta ttagatgatg ctggctacaa agatgtggat gacgatggtt
     5221 taagagaaga caaagatgga aataaactaa caatcaactt tgcttcgatg tccggtgggg
     5281 aaacagcgca accattggca gattactata tccaacaatg gaaagaaatt ggtttggatg
     5341 tccaatacac aacaggtcgt ctgatcgaat tcaattcttt ctatgataaa ttagaaaacg
     5401 atgatcctga aattgatgtt taccaagctg cttggaatac aggaactgat ccaaatccaa
     5461 gtggactatg gggaccaaat gctgcgttca actacactcg ttatgaatca gaagaaaata
     5521 caaaattgat caacgatatc agttcaagca aagcatttga tgaagagtac caaaaagaag
     5581 catatcaaaa atggcaacaa tatgcatttg aagaagcatt tgcgattcca acgttgttcc
     5641 gtaatgaagt attgccagtc aacgaccgtg tcaaagactg ggattggtct tatgatgtac
     5701 caaacccatg ggcaaatgtt tctgttacag cagattcacg ttcataatca ctaaaaatga
     5761 ccgaaaaagg gcgatgtcct ttttcggttt tttgatattt ttctgtttat ttctctaata
     5821 ttcaggtcaa ggcgctgatt tcatgacttc tccaataaac ggtgttcaaa gtccagtttc
     5881 tcggtattaa gtcaaattta tgcaaaaata tggaaagcca ttttcacaaa attttttctt
     5941 catactcgaa actaaacgaa tttttcacaa cttctcgtca atcactagga ttattaaagc
     6001 tttacttacc ttttgaccga accatttcaa atttgacaaa aaaccagtat aataaggaag
     6061 tatcgaaaaa atatccataa aaggaatgtg gaaaatggac aaccagctga caaaagaact
     6121 aaaagaaaac tataacatcg tttttcacga tctaaatttg cttgaacaag cttttaccca
     6181 ttcatcctat gtgaatgagc atcgcaatct gcaattatcg gataatgagc gacttgaatt
     6241 tttaggagat gccgttttag aactgatggt ttctcagtat ttattccgtt tatatcctca
     6301 tttgccagaa ggaaaattga caaaaacacg agctgcgatc gttcgcgaag acagcttgtc
     6361 gaaatttgcc aaagaatgtc actttgatca atatgttttg ttgggcaagg gagaagagaa
     6421 ttcaggagga cgtacgcgac cagcattgct ttgcgaccta ttcgaagctt ttttaggcgc
     6481 gttatatctt gaccagggat tcgacacgac ccatgcattc atcgaaaaag tgatttttcc
     6541 taaagtcaaa gcaggtgctt tttcacatga gatggatcat aaaacaaaac tacaagaagt
     6601 cttgcaaaaa tcaggtgatg tatcaatcga ataccgattg atcaatgaag aaggtccagc
     6661 tcatgaacga gtattttgga tcgaagttta tgtagatgat caattgattg gcactggaca
     6721 agggaaatcg aaaaaattag cagaacaagc tgcggcggaa aatgcattgg cagcactgta
     6781 aaagtaagtg agggaaagct ttgtatttga aacgaattga aatagccggt tttaaatcat
     6841 ttgcagacaa gacagtcatt gattttgaaa atagcgtcac agcagtggtt ggaccaaatg
     6901 gcagcggaaa aagcaatatt acagaggcta tccgctgggt attaggagaa caatctgcta
     6961 aaagtctgcg aggcggaaaa atgccggata tcatctttgc cggttccgat acccgtaagc
     7021 agctgaatat tgcagaagtc acagtgattt tagacaatag tgatcattat ctgccattgg
     7081 agtacaatga gatcagtgta acccgccgtt atcgacggac tggggaaagc gagtttttca
     7141 tcaataaaca aagctgcaga ttaaaagata tccaagaatt gttcttagat tctggattag
     7201 gaaaagaatc gttctcaatc atttctcaag gaaaagtaga agcgattttc agtagtaaac
     7261 cggaagaccg tcgtgggatc tttgaagaag cagctggtgt cttgaaatac aagcagcgga
     7321 aaaagaaagc agaacagaag ctttttgaaa cagaagacaa cctcagccgc gtccaagaca
     7381 tcatccacga attggaagaa cagttaactc ctttggccgc ccagagtgag gcagcaaagg
     7441 agtttttacg gctaaaagaa acactgactc aaaccgatgt ctctttgatg gttgctgaaa
     7501 tcaagacagc aaaaaaagat tgggacaaca aacaagcaca gcttgcgaaa ttcaatctag
     7561 aattaggcaa actatctgag tcgatccaag aacaagaatc cattttggcg aagcagcgta
     7621 aagaaaatgc gcaagccgat cgtctgatcg aaaaaaatca gcaagtatta ttagatcttt
     7681 cggaaaaact gaagcagaca gaaggacaaa aagatgtttt gcaggagcgg acaaagcata
     7741 cacaaaaaag ctcgcaagaa tatcaaactt cattagcaga agcacaaaaa aaggttaaac
     7801 attttgaaaa gcttcaagaa agcttgatga aagcagcagc agaaaaagaa acagagatcc
     7861 aaaaagctga agcaaatctc ataaaaacac agcaagaatt agaaaaatac caaaaatcaa
     7921 cgaaagaatt attggcagaa ttaagagatc aatatgttga tttgatgcaa gaacaagcag
     7981 ctgtaggaaa cgaactgaag tatctagaac gtcaatatat acaagaaaca gcaaaaagca
     8041 agcagacgtt agccaagcaa tcggaagtag aagcatcagt agaccgattg atcttacaaa
     8101 aagaagaatt gacacaaaag caggcgcagc taaaatcatc tttaactgaa acaaaagaga
     8161 agttagaaat gatccaacaa aacgggaaaa agttccaaga agcattagca aaagaacagc
     8221 ctaaaatgta tcaattaatg aatcaagtcc agcagctacg tgctcgtaag aaaagccttc
     8281 aagaaatcca agaaaactat tttggttttt accaaggggt tcgattggtc ttgcagcata
     8341 aacagcagct ttcgggtatt gtcggagcag tggctgaact gatcgatgtt cctgcagatt
     8401 tcacgttagc tatcgaagca gctttaggtg gggcagccca gcatgtcatc gttgaaaatg
     8461 aaaaagatgc tcggcaagcc atcacgtatt tgaaacagca aagaggagga agagcaacat
     8521 tccttccact gacgacaatc aaaccaagac aattaccggc tcatatattg acacaagcag
     8581 cagctgtaga agggtttata gggattgcca gtgaacaagt cagttatccc gatcaaatcc
     8641 aaactgtggt tcaaaacctg ctaggaacga tccttttagc caaagactta actagtgcca
     8701 atgcaatcgc gcaaacgatc cgctatcaat atcgagtggt ttccttagaa ggtgatgtga
     8761 tgaatgccgg aggatccatg acaggtggag cgaataaacg aggaaaccaa ggcagtttgt
     8821 ttgtacaaaa tcaagaattg aaacagttga ctagtgaatt tgaagaagca gataaacaac
     8881 tccaagcaca agaaaaaaaa gttcaggagc tgcaacaaga aacagctcgt ttagccgaag
     8941 aacaagaagt tttgcgtaca cgaggcgaac aattacgttt tgaagaacaa gaagcgacaa
     9001 atcagctaca aaacatcata aatgaacttg aacgttttga aaaagaaaag caaatctcaa
     9061 cttttgagac acgtgaactg cagcagttta tcgaagatta cgaaaagcaa acgaacgaat
     9121 tgaaagacaa acaaacagat cttgaatcgc aacgacaaaa aatcgacgaa gaaatcaaat
     9181 cgttaagtca agaaagcgac cagatggaag ccagaagagc acaagtacaa agtcaaaaag
     9241 cacaagaaca agcagatctt gctgtcttga aagagcagtt taatcatctg cagatccagc
     9301 tccgcggtgc ccgagtccaa aaagcagagg caaccgaacg tcaagaagcg atagaaaagc
     9361 agttggcgac attgacagca gacttttctg atcatgaagt cacagaagaa agtttggaaa
     9421 aacaaattaa cgaattatct gctcagcgag aaacattaaa agccgagttg gcaaaagcca
     9481 aagagcagcg agatagaagg caaaaggaga tcgataagct tgaagcagtc ttggcagagc
     9541 gtaatcgtga gcaaaaagca cgactgtcgg aacaatccaa attagaagtc caaaaagacc
     9601 gagctgaaat gattttagat aatcatttga cctatctgca gtcagaatat cagctgacat
     9661 ttgagaaagc ttcccaagac tatcaagaaa caaccgatat cgaagacagt agaacaaagg
     9721 tttcatcctt aaaagaacag attgaaaaac tgggaccagt aaacttaaat tcaatcgaac
     9781 aatacgaaca agtcagtgaa agacatacat tcttagcaac acagcgagat gatctgttag
     9841 cagcgaaaaa ccaattgttt gaaacgatgg atgagatgga tgatgaagtg cggacaagat
     9901 tcaaagaagt atttgaagct atccgtcaag aattcaaaat tgtctttcca aatatgtttg
     9961 gcggcggtaa agctgaatta gtactgacgg aaccaactga tttgctgaaa acaggtattg
    10021 agattgaagt acaacctcct ggcaaaaaac tgcaaagtct aagtttgctt tctggaggag
    10081 aacgagcact tacagccatc gcgttgctct tttccattat ccgtgtctgt cctgttccgt
    10141 tttgtatctt agatgaggta gaagcagcgt tagatgaagc caacgtgaaa cgttttggtc
    10201 gttacttgag cgatttccaa gatgacacac aatttatcgt agtcacacac cgcaaaggaa
    10261 cgatggtcgc agcagatgta ctgtacggag tgacgatgca ggaatccggt gtatcaaaaa
    10321 tcgtttctgt acggatggaa gatatcaatg aagaggcgaa aatcaacgaa acgaaagtgt
    10381 aggaacgaaa atgatcaaat tagtagcaat cgatttggac ggtacattat taaatgaaca
    10441 aaagctgatt tcagatgaga ataaacaagc attagcacaa gcaaaacagc aaggggtcaa
    10501 gatcgttcta tgtacaggac gccccttagc tgcgatggcg cattatctgc aagaattagg
    10561 actggtagat gaaggtgact acagcatcac tttcaacggc ggtttggtac aaaaaaatga
    10621 tactggtgaa atcatggaaa agaaagtgat ggaagtatca gatatccaac gcttatacaa
    10681 actggctcag caattggatc tgccactaga tgttttgtct gacaatgtgg ttttacagct
    10741 gcctagtgcc ccaaggaagc aatccttgta caatgtgttg aataagctgc tgcaattcca
    10801 accagcggat ataagcgata ttacagatga atttgtgctt aataaggcag taatcgctta
    10861 tgaccaagaa gaattagatc ctaaaatcaa agaaatacca gcagaattcc atgaaatgta
    10921 tgagatcatc aaaacaagaa gtatgctttt ggaatttatg ccaaaaggtg ttacaaaagc
    10981 ttatggtatt tctttattgg caaaagatct tggtttgaaa caagaagaaa tcatggcgat
    11041 cggtgatgag gaaaatgatt taccgatgat ccaatatgct ggactaggcg tagcgatgga
    11101 gaatgctgtg cctttcgtca aagaagcagc agatttcgtg acagccagca atgaagaaaa
    11161 tggcgtagcc aaagtgatcc aaaaatttgt attagagtag ggggagtcaa atgggatttt
    11221 ttgataaaat caagcgtgct tttactggcg aagagaaaac agaagaagtt gaagtacagg
    11281 agaagtacga caagggttta gaaaagacgc gtaaaacatt tggtgaacgg atgaatgagt
    11341 tgtttgccaa ttttcgaaca gtcgatgaag acttttttga agaattagaa gaaacactta
    11401 tcggtgcaga tgtaggtttt gaaacagcga tacgaatcac cgaagcatta cgtcaagaag
    11461 tgaaactcag aaatgcgaaa aaatcggcag aagtccaaaa tgcgatcatc gagaaacttg
    11521 tggatctcta tgaagcggaa ggaatcaatg aagtaaatga actaaatatc caagaagacg
    11581 gactaacagt tattttgttt gtcggtgtta atggtgtagg gaaaacaaca agtattggta
    11641 aacttgccca tcagttcaaa aacgaaggta aaaaagtatt gatggcagca gcagatacct
    11701 tccgcgctgg agcaatcgac cagttagttg tctggggcga acgcgctggt gttgaagttg
    11761 ttcgtggcaa tgcaggaggc gatccagcag ctgtcgtatt cgatgctttg gaaagagcaa
    11821 agacagagca ggcagatgtt ttactagttg atactgcagg ccgactgcaa aataaagtga
    11881 acttgatgaa cgaattagaa aaaataaaac gagtaatcca gcgggaatta ccagaagcac
    11941 cccaagaaac acttcttgta ttagatgcaa ccactggtca aaacgcgatg gttcaagcaa
    12001 aacaattcaa agaaacaaca gatgtgaccg gtcttgtttt gacaaaatta gatggtacag
    12061 caaagggcgg tatcgtactt gctatccgta atgaattgca tttaccagtg aaattggtcg
    12121 gtcttggtga aggaatcgat gacttggagc cgtttgatcc aaatgatttt gtcgttggat
    12181 tgttcaaagg attactaaaa gaagaataac ccatgtctaa aaagcaggag ctgtgacaaa
    12241 agttgcagct ccttgtttga atgattgttt ttaacatagc tgtgtttttg atgccttcat
    12301 ttttatactt atttgtaagc ggttctcaaa caatttcgtt ctttttctta gactttttga
    12361 ttataattag attcatatga attggaggtc acttaatgat gaattttaag caattattat
    12421 tgcatgtcaa tgcgcgccct tttatcgacc aagctcgatt tggtatagaa cgggaaggac
    12481 agcgggttga tcttgcagga aatctagcaa aaaccgatca tccagcaatc tttggcgatc
    12541 gatcctatca cccctatatc caaacagatt ttagcgaaac acaaacagag atgatcaccc
    12601 ctgttaccga ttctattccc gaattatttc agtatctcgc tgctgtttat gatgtgactg
    12661 ctcgttctat accgaaagaa gagatgatct ggccattaag tatgccacct gccttaccgg
    12721 aaaaagacga agagatcatt attgcaaaat taaaaaattt cgaagatgtc ttgtatcgac
    12781 gttatttagc aaaagaatac gggaagcgaa aacagatggt aagcggtatt catttcaatt
    12841 ttgaatttgg cgatgagttg ctaagaacat tgttcagcca tcaggaagaa tttcaagatt
    12901 tttccgaatt caaaacagaa ctttatttga aaacagcgag aaactttatg cgctatcgct
    12961 ggatgatcac ttatttattc ggtgcttcac caatgagtga gaagaactac tttttagatg
    13021 aatcacatcc gcaagaacct gttcgcagta ttcgcaacag tgcgctgggc tatacgaatc
    13081 atccaaatgt gaaagtttcc tatgcttcta tgaaacagta tttagcagat atcgagcgaa
    13141 tgatcgaaga agggaaactt tcggaggaga aagaatttta tacaccgctt cgtttccgag
    13201 gcggaaagaa agtcgcagat ttagcaacaa caggtgttcg ttatattgaa ttgcggaata
    13261 tcgatttgaa tccttatgca agattgggta tcaatccaga gcaagttcgg ttcttacaat
    13321 tattcctgat gtacatgtta tggacagaag aaaaagaaga ctgtgaccaa tgggtagcag
    13381 aaggtacgac tcgaaataac aaagtagcac tagaacaacc atcagatcaa acagaatttc
    13441 atcaagaagg tagagaaatt cttgaaggaa tgaagcaaat gctggttgaa ttggattggt
    13501 tggattctct ttatttagta gaggaagcat tgactcaaat ggatcatcct gaacaaacgt
    13561 tagcagcgaa actctatcaa gaagcacagc tatcgagtca gcaagaagtt gctgtggcat
    13621 tgggacatca atattataaa gaaagccatg aacgtcccta tcagttggcc ggttttcgcg
    13681 aaatggaact ttctactcag atatttatgt tcgatgccat ccaaaaaggt gtccaagtca
    13741 aagtattaga tgagtcggat caatttttgc gtctgcaatt ccaagaccat gtagaatatg
    13801 tgaaaaatgc aaacatgaca agtaaggaca gctatatcgt cccacttatc atggaaaaca
    13861 aaacagtaac gaaaaaagtc ttaaaagaag ctgggttccg ggtaccagga ggcgcagaat
    13921 tttcatccat ggaagaagcg gtaaaagctt atccgagatt tgccgagcag gcatttgtga
    13981 tcaaacctaa atcaaccaat tacgggttag gtatcacgat tttcaaggaa ggagctagtc
    14041 tggaagatta tcaagcaggt ttagccattg cttttcgtga agacagttcg gttttagtag
    14101 aagaatttat gccgggaacg gaatatcgtt tctttgtgat cgacggagaa gtccaagcca
    14161 tcatgttgcg agtccctgct aatgttattg gtgacagtat ccgaacagtg aaagaacttg
    14221 ttgaagaaaa aaatagtgac cctttgcgtg gaacgaatca tcgtgcacct ttagaattga
    14281 tccaattagg tgaattagaa caattgatgt tgaaagaaca agggttgacg atcgaatcag
    14341 ttccccaagc caatcaaatt gtttatctaa gggagaactc aaatatcagt acaggtggtg
    14401 attcgatcga tatgactgat gaattttctg aagcttacaa aaaaatcgcc gtttctgcgg
    14461 tagaagcact aggagctaag attagtggta tcgatttgat tataccagat aaagaaatcg
    14521 atcctacaac tgataaaaaa gcatatggga tcatcgaagc aaatttcaat ccagccatgc
    14581 atatgcatgt ttatcctttt gcaggaaaag gcagaagatt gacgatgaat gtattgaaat
    14641 tgttatatcc tgaagtattt taaaagaaaa agcttttgac agattgtcct atttgatgtc
    14701 aatctgatca aaagcttttt ttgcatgtaa aaatttatgt gttttttcgt tttaaaataa
    14761 acgatcccag aagctttttt tcttttttgg tttttctttt tcagctggcg aagagttttc
    14821 ttctgggtat tttttttcga tatcaatcac aggttcattt actgcttctt ttgctgttaa
    14881 gagcaatccg aattcctcag gatttgcagt gacgtaatcg ttgactacag tgaaacggct
    14941 attgtttttc gttgctagct ggatatattg gttttggaca gaaaggggta gagccccatt
    15001 caagagtatg gaagcatctg gatgtttttt gagttctttt aaaaagtttt ctttgttttt
    15061 tggttggatc atttcagcaa cagtcatact aagatagcag cgttccctga atgtccctaa
    15121 atatttatgc tgttcttcag ggttcactaa aggtgtacca taaagtccct tatccaaatg
    15181 gctttttaca tcatcttgtg ccataaaaaa taccctcctt cttctctttg tctttcttac
    15241 gattatagca tattcccaat aatacctaaa acatatgaaa aagcggttgc ttttgttcaa
    15301 cgattcactt aaaataagaa tgaaaatcat tctcaattag aaaggtggat tggatgcgct
    15361 acggacgact agtagcatta ttagttattt taagtagtat ttcattgttc atcggagtat
    15421 cgaatgtaca attgtcagaa ctgctccagt tagatgggca aagcttacaa atcttgctag
    15481 tgactcgttt gccacgaacg atttgtttga tccttgtggg agcgaccagt agtatttgtg
    15541 ggttgatcat gcaacatttg acccaaaata aatttgtttc accaacgact gcgggcacga
    15601 tggatagtgc gcgtttaggt attttagtgg ctatgatttt cctacctggt gcatccttga
    15661 ttgtccgatc cttgacagca ttttgctttg cttttgcagg aacattgctt tttttatccc
    15721 ttacgcgatt gttcctccag aaaaatcaat tgatgcttcc gttgattgga gtcatgttag
    15781 ggaatatcat tggttcagtt gctacttttt ttgcttatca attccagttg gtacaaaata
    15841 tgacttcatg gcttcaaggg aattttgcga ctgtgatacg aggaaattat gagttattgt
    15901 atgctgttgt tccattgttg ctcttggttt ggctatttgc ttaccgattt acgatcattg
    15961 gtctgggcga atcgtttgca gtaaatctgg gaatagatta ccagaagatg cagtttttag
    16021 gcattggctt agtatcattg gctagtgcag tcatgttgat catggtagga agcattcctt
    16081 ttatcggagt gatcattccg aatcttgtct ctagaatata tggagatcaa gtgcacaaaa
    16141 caattggcgt aactgcttta gcaggtagtt tgttcttagt attttgcgat cttgtcgctc
    16201 gcgtgttgat ttttccttat gaaataccag tcagtgtgat cgttggaatc atcggtgggg
    16261 ctatcttcct ttatcttctg ataaggggac gacgatatgc ttaaaaaaaa gccatctatt
    16321 tttattggta tgcttgttct tgttttattt gttacaggaa tgtacttgac gatccataca
    16381 tatggaaact ggcattttgc atgtgcattg aggagtaaga aaatttttgc atttatcctt
    16441 gtctcgctag ctgtcagcag tgccacaatc agctttcaga caattacgca taaccagttt
    16501 ttgactcctt cagtattggg acttgagtcg ttatatgtcc ttgtccaaac ggtccttttc
    16561 ttctttatcg gaggagtgac gatgcttcag cagacaggta cagttttttt cttattgaac
    16621 gttgggctga tggtcgggct gagtctattg ttatttttct ttcttttgaa aaaaatgaga
    16681 gcgaatcttt ttttattact gatgtcaggg atgattttag gtacgctttt tggtagtgtc
    16741 agtacctttt tacaagtcat catggatccg aatgaatatg atttgcttca aggcagatta
    16801 tttgcaagct ttggcaatat ccagagtcag tatcttttgc ctgcttcttt gatcattatc
    16861 ggctgctggt acctgttatt tcgatatcga catgtgctag acgttttcca tctcggaaat
    16921 gaacaagcag taaacctcgg gatagacaca gatcgttttg aatttttgat tttatgttgt
    16981 gtcagtcttt tgacagggac atcaacggct cttgtgggtc cagttacttt tttagggttt
    17041 attgtcgcaa atatcagtta tcgtgtcttt tctacttatc gtcatggtca gctatttatc
    17101 ggaagctttc tggtagctat gctgtttctg acaggaggac agctcgcagt agagcagatt
    17161 ttcggctgga atacaacagt gagtgtcgtc gttgaatttg tcggaggaat ttattttgtc
    17221 acaaaattga ttcgcgaaag gaagagtatc ctatgattga tttacaaaag gtcaataaac
    17281 tgtaccatgg aaaatcagtg gttaaagatg tacagttaac gattcctgaa aagaagttga
    17341 ctgcttttat cggtccaaat ggtgctggaa agtcaacggt gttgtcgatg atcagtcgtt
    17401 tgataccaaa agatacaggt gaaatctatt tagaccataa cgaagtaaag gcttggaaat
    17461 ccaatgaatt agccaaaaaa ttatcgatcc tacgacaatc gaatgaaata aatgtccaaa
    17521 tcaccgtgaa agaattagta gaatttggac gatttcctta tacgaaagga aaattgacga
    17581 caaaagacga ggaaatcgtt caggaatcac tggaacacat tgggttagaa gaaatagcgg
    17641 atcaatcgat tgacactctt tcaggcggtc aactccaacg tgcgtatatt gcaatggtgc
    17701 tagcgcaaga cacggattat attttactgg atgaaccttt gaacaatcta gatatgaatt
    17761 ttgcggttca aatcatgcag attttaaaaa atttagtaca agaaatcggg aaaacaattg
    17821 ttattgttct tcatgatatc aactttgctg caagttatgc agacgaaatt gttgcaatga
    17881 aagatggaca gttatttacg caaggaacga cccaagaaat gataaaaaaa gatgtattgg
    17941 atgaattata cgaaatggac atacggattt gtgagattga aggccgcaga ttctgtcttt
    18001 atttctaaaa ctaacattag gagagaagag atgaaaaaat tagtagtagg tatcttgatt
    18061 ctagcatcat ttggtttagc agcatgtgga aatagcaaca acaccagtca ggcagacaca
    18121 aaaaaaagca gcacacaaac tgaaactaca ttgacgataa cagatagtaa tggagaccaa
    18181 atcgaggtgc caaataatcc agaaaaagtc gtcgtattcg acaatggttc tttagatacg
    18241 atggatgctc ttggtgttgg agataaggta gttggagcag ctacaagcag ccttcctgag
    18301 tatctttcat cctataaaaa agtagaatca gcaggtggaa taaaagaacc agacttagaa
    18361 aaaattaatc agcttcagcc agatctaatc atcatttctg gccgacaaag agattttcaa
    18421 aaagatctaa gtcaaattgc tccgaccatt ttcctttctt tagatgcgaa aaatccatgg
    18481 gaatctttcc agcaaaatgt cactgcttta ggggagatct ttggtaagca agaagaagct
    18541 aaaactcaat tagaggaatt atctagtgca atcgatcaga cgaaaaagaa agcagaagct
    18601 actgacaaaa aagcactagt cacacttgtg aatgaagggc aattatcagc ctacggatct
    18661 ggttctagat tcggtttcat ccatgattta tttggctttg aacaagcaga tgatcaaatc
    18721 gaagcatcca ctcacggtca aagtgtttct tatgaatatg tgctggaaaa aaatccggat
    18781 atcttatttg tagtagacag aacaaaagct attggtggag acgacagcaa agatgatatt
    18841 tctgccaatg aactggtagc acaaacgaat gctggtaaaa atcagcagat catctccctt
    18901 gaaccagatg tctggtattt aagcgggggc ggtttagaat caatgaaact gatgatcgaa
    18961 gatgtgaatc aagcatttaa atagcataaa taacagagga ttaaactctc atactatgta
    19021 acaataatgt ttcttacatt cttaaatagc aagagcctat cctcaaagaa gttatcaaaa
    19081 accatccgta aaatcgtatt gatgatttta cggatggttt ttatttattt gagaaatctt
    19141 ttttcaacgt gaattcatta tctacatcat acgggttgat tctagaatgg ggagtggcaa
    19201 aaaatagtgt gatccatttt ttcagcatca agatcgcctc ctttgatttg aatcaaaggt
    19261 acaaagcgaa acttagataa aacttaaatt tagatatttt tattttgtac cgatctcttc
    19321 tttaaacgat gttccatctg ctgcttctcg gcaataagtc cactagacta gaaaatatga
    19381 gaagctattt tctgtccagc gttcttatta ctcaaaacaa atcgcagttg tcacaatctc
    19441 ttctaaacga tgttccatct gctgcttctc ggcaataagc ccactagact agaaaatatg
    19501 agaagctatt ttctgtccag cgttcttatt actcaaagca aatcgcagtt gtcacaatct
    19561 cttctaaaca atgttcaatc agctgacctt cggtattagc tcaaagcact gatttcacaa
    19621 cctctattta ggcatgtgtc tcagcaagcg gttgatagtt gatgatctgc atttttcgat
    19681 aaaaatcggc gggaagctta ctccaccgtt ttagtaattg atagaaataa ctctcttctg
    19741 ctttgatttt atactcgctt aatatcaatc caaaactatt cgtttttttt gaagctaatt
    19801 ttttataaat atccaatgct ttggttttat cttcattgac aaagagtgtt tttacttcaa
    19861 tataagaacc ctcgatattt tgtgtttctt cgataaaata gatgtttttt atatattcct
    19921 tcatagtgat tcctccaaag aagtctttta tttgataagt ttactctacc gaagagttgt
    19981 taataatccg tgaacaaaaa gtaattcttt ttccaatagt tgttaaaggt tttttatttt
    20041 ttatagtatt ttttgttatt taagaaagga ataaggttaa acaaataaca ggttgctttt
    20101 tgggtctttt tccgttcaag aaactgaacg aagaaaaggg aaagttgtct cttttgtgat
    20161 aagagaaatc ataataaacg aatgacttac tttccaataa attgagaaca gtcagaacca
    20221 gacaaattag ttgcatctga tgaataaagt gattattcta tgacatgagg tgatacagat
    20281 gaaattagaa gtgacaaaag aagcacaaga agtattgaag aataaattag aagcagacga
    20341 tcaactttta ttagatatcg aaaacggtga cggtccattt gcagaaagcc aagtcagttg
    20401 ccaattggat acggcatttc gattgatcat tgtcaaaaaa gatacacaaa cagatctaag
    20461 tctttattct gtagtggctg atacaccagt tggaccaatc aaaatcaaag acagtgcaat
    20521 actttacatg gatgatccat ccacacttgc tttagaacca acttataata gtttacaatt
    20581 aaaaggacca agcggcttga gaaaaggcaa cttgcaagtt gttcgaaaag actagaggga
    20641 taaaatggga aaattggcga tcatcagcga cctacatgtg gacatcaata aattcggcga
    20701 atcagagctg atgcagcttt ggcaagtttt gcaaaagcac aagatcactc ggcttcatct
    20761 agccggagat acggctaata aagtagaccg ttgcctagct gttgtcgaat ttttcgtcaa
    20821 taaaggatta tctacgacat ttcatttcgg caatcacgag ttggcagatg tgcccaatga
    20881 acagatgatg gaccattttc cagacccaca ttttttaaat gaacggtata ttgctttaaa
    20941 tgagcagacc gttcttttag ggatgaatgg ctggtacgac tatcagtttt ccgaattgaa
    21001 agattacgag gcaatcgtac gaatgaagcg tttgtattgg tatgatcggt tcatccagcg
    21061 tgggaaaaca gatctcgagg tcaatgcaga aatgcttgca gctgcaaaaa atttgctaga
    21121 ccagctggaa gcaaaaaaac ttgaggtgat tttatccact catttcgttc ctaaacgggc
    21181 atttatcatg tatcaatctg ctccttatga gcgttggaac aaattaaatg catttttagg
    21241 atcagaatca ttcggagaat tacttgaccg ttattcaaat atcaaacaag tagtttttgg
    21301 ccatacgcat cgtcgctttg aagataaatc catcggtcac acgatttaca gctgcagacc
    21361 ttttggttat tattatgaat ggcagctgac ccgagatttt gtcttgaaag aacagttgct
    21421 agacagctat gatcctatga aattacgtgt cctcttgcgt gcccatcacc cagcttttca
    21481 ggcctatcaa acacaacatt tgcaagagga atttgaacaa gcgatgacaa tcgttccgta
    21541 ttgagacagc attagtaatg tgtaaaagaa aataccttgt aaaacataag gataagtaag
    21601 agcagtagat ccatttatcg tggatatgct gctttttttg cggaaaataa tcagcgaatg
    21661 tttatttgat cggatgattt gtaactcgca gagtactaga tgcaagcgtg tattcttttt
    21721 ttgaacgata atcacaaaaa aaaatataat atacattttt tatttaatta taattaatat
    21781 tgattgaagt ctaggcagtc aagtcgagag t
//
LOCUS       DAHUVV010000041        21665 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.041, whole genome shotgun sequence.
ACCESSION   DAHUVV010000041 DAHUVV010000000
VERSION     DAHUVV010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.041"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            72..506
                     /locus_tag="NK767_001523"
     CDS             72..506
                     /locus_tag="NK767_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6694523.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(584..1117)
                     /locus_tag="NK767_001524"
     CDS             complement(584..1117)
                     /locus_tag="NK767_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCH6694524.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1211..2389)
                     /gene="mutY"
                     /locus_tag="NK767_001525"
     CDS             complement(1211..2389)
                     /gene="mutY"
                     /locus_tag="NK767_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCH6694525.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2382..3179)
                     /gene="recX"
                     /locus_tag="NK767_001526"
     CDS             complement(2382..3179)
                     /gene="recX"
                     /locus_tag="NK767_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCH6694526.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3286..4668
                     /gene="rlmD"
                     /locus_tag="NK767_001527"
     CDS             3286..4668
                     /gene="rlmD"
                     /locus_tag="NK767_001527"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCH6694527.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4842..5420)
                     /locus_tag="NK767_001528"
     CDS             complement(4842..5420)
                     /locus_tag="NK767_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HCH6694528.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5742..6212
                     /locus_tag="NK767_001529"
     CDS             5742..6212
                     /locus_tag="NK767_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HCH6694529.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6320..7546)
                     /locus_tag="NK767_001530"
     CDS             complement(6320..7546)
                     /locus_tag="NK767_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCH6694530.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(8300..8500)
                     /locus_tag="NK767_001531"
     CDS             complement(8300..8500)
                     /locus_tag="NK767_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCH6694531.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8634..9242)
                     /locus_tag="NK767_001532"
     CDS             complement(8634..9242)
                     /locus_tag="NK767_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCH6694532.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(9327..9797)
                     /locus_tag="NK767_001533"
     CDS             complement(9327..9797)
                     /locus_tag="NK767_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCH6694533.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9845..10759)
                     /locus_tag="NK767_001534"
     CDS             complement(9845..10759)
                     /locus_tag="NK767_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCH6694534.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10905..11708
                     /locus_tag="NK767_001535"
     CDS             10905..11708
                     /locus_tag="NK767_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HCH6694535.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11886..12833
                     /locus_tag="NK767_001536"
     CDS             11886..12833
                     /locus_tag="NK767_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCH6694536.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12901..13194)
                     /locus_tag="NK767_001537"
     CDS             complement(12901..13194)
                     /locus_tag="NK767_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HCH6694537.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(13222..13887)
                     /locus_tag="NK767_001538"
     CDS             complement(13222..13887)
                     /locus_tag="NK767_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCH6694538.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(14027..14659)
                     /locus_tag="NK767_001539"
     CDS             complement(14027..14659)
                     /locus_tag="NK767_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6694539.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14666..15733)
                     /locus_tag="NK767_001540"
     CDS             complement(14666..15733)
                     /locus_tag="NK767_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCH6694540.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15730..16461)
                     /locus_tag="NK767_001541"
     CDS             complement(15730..16461)
                     /locus_tag="NK767_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCH6694541.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16630..17109)
                     /gene="greA"
                     /locus_tag="NK767_001542"
     CDS             complement(16630..17109)
                     /gene="greA"
                     /locus_tag="NK767_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCH6694542.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(17245..18420)
                     /gene="mltG"
                     /locus_tag="NK767_001543"
     CDS             complement(17245..18420)
                     /gene="mltG"
                     /locus_tag="NK767_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HCH6694543.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18624..19793
                     /locus_tag="NK767_001544"
     CDS             18624..19793
                     /locus_tag="NK767_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCH6694544.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(20060..20974)
                     /locus_tag="NK767_001545"
     CDS             complement(20060..20974)
                     /locus_tag="NK767_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694545.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQG
                     TYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTF
                     KSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFS
                     KISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(21032..21665)
                     /locus_tag="NK767_001546"
                     /pseudo
     CDS             complement(21032..21665)
                     /locus_tag="NK767_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         6697 a         4230 c         3440 g         7298 t
ORIGIN      
        1 tttgaaaact tgccatactt tttatttgtt ttattttcca gtatactata cgaaaggaag
       61 gtggctagac catgaaagtt gtacaaacaa aagatacaat gagcaatatt tacttagatg
      121 ctgtacggat caggcatcaa gtttttgtag tcgaacaggg tgtgcctttg tctcgagaaa
      181 tagacaaaga tgaagcccat tgtatccact tcgtcctgta ctcagataaa aaagaaccgc
      241 aaggaactgt acgtcttttg cctttagaaa acggaaaaat gaaattacag cggatggcga
      301 ttctttctga atacagacac caagggttag gaaaaatatt gattgaagaa gcagaaaact
      361 ttgctaagaa tcaaggctat aatacgattt tattaggggc acagtccaca gctgaaactt
      421 tttacgaaaa actaggatat acagcttacg gcgatccttt tgaagatgct ggcatgcctc
      481 atatttatat gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa aacagcaagc
      541 ttttgagaaa aatctctcac tggcttactg ttcttttttg tgtttactta cgcgacaatt
      601 gcttgtaacg gttataccaa atatcaatat agccttccga gaacgggcca tctccattat
      661 tgatccagtc aaccagaatc ttcacatttt cttttaggat gaagtcaata tcgttcgggt
      721 aattcatcat tttgctgtgc atctcatatt catcgacatc caatagtcgt ttctcgccgt
      781 caggaaatac cttgatatcc aagtcatagt cgatatattt caaggcctca tcgtccagca
      841 aaaaaggtga agctagatta caataatagg aaaccccctt ttctctgatc attgctatta
      901 cattgaacca gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt acccatcggc
      961 gtccatcaga ttcagtgact aatgtgtgat cattcacgcc gatcatagag tgttcgcttg
     1021 tttttaatac catggtatct cgccacgttc gatgcaattt gccgtcgtgc ttgtaacttt
     1081 ggatcgtcac aaactctcct tcttttggaa ctcccatagc aacccttcct taagcagcat
     1141 tttgtgcttt gtgtttgggt gttcattaca ccgacatcat aattattata tcaataaaaa
     1201 tgagctttgc ctagaaagga ttcctattgt ttcgattttt ctctaactga tctaccagtt
     1261 tcatttggac cttaggaaaa acgaccgaat caaatgcatc aggcttcaac cacttcgaag
     1321 ttttgttctc ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa aaaaggagaa
     1381 catgccattt cagatgacta aaaatatgag taacttctcc taaatgtcgt gtctgccaaa
     1441 caaagagttc ctgcttttca aatgggagat tttgctcttc ttctgctact aggtcatcaa
     1501 acagatcgat ttctggcttt gtttcccatt cttttttcaa tcgctcatac tcctcttgtg
     1561 tcacttccat cattggaaat gtccacatat tcgccagcaa tttttggcta tctctttctt
     1621 caaaataata cgctccagaa tgattttgaa gagctgcact gatataataa acgtcttttg
     1681 gctttgcttt ttttgttttt acaggaaaac tcgtttggat tcctcgtttg ttagctaaac
     1741 aaaaggcttg gatcggacaa gtctcacact ttggagaagt tggtgtacag atagctgaac
     1801 ctagatccat cattgcttgg ttgaattcac ctggatgctt ttcatcaatg attttccgca
     1861 tcgcttcatc aaagattttc ctgctagaag cttttgcgat gtctgcttca atgcaaaaca
     1921 atctgctgac tactcgcatc acgttgccgt caactgcagg ttctggaagt ccaaatgcga
     1981 tacttgcaat cgcgcctgtc gtatatggtc ctattccctt caatgagcta atttcttcag
     2041 gcgtttgcgg catttcccca tcaaattctg acatgatctg tttagcagca gcttggatgt
     2101 tccgggctct tgagtaataa ccaaggccct cccatgcttt taaaagcttt tcttctggag
     2161 catttgctag ctcttcaatt gtaggaaacc attccatgaa acgataaaaa taatcgatca
     2221 ctgtgtctac tctggtttgc tgaagcatga tctcagagat ccagatacga taaggatcac
     2281 gattatagcg ccaaggcagg tttctttttt cttgttcata ccattgaatg aactgatctt
     2341 gaaattcttt tgtttcttta tctgtccatt tttcccattc tttattcact ttcctcttcc
     2401 ttttccgtaa tgaatcgctg gatcatatcc aaatcaaatc ctttttgata taagctttgc
     2461 tttactttta gatttctttt tttaggttca aaccttaaat ttcttcgcca tagtttatct
     2521 ccttgtttga ctaaataatc atattctatt tcttgatctg cttcttgcgg aagatcagct
     2581 atcgcttgtt ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg ttggattttt
     2641 ctcaataatt ctttttggct ttttccgtga ttttttccaa acgctttttc ggctgtctga
     2701 tgtgcatttt gaacttggtc ttcaaatgta tattggctga gcgcttgttc aatgatttca
     2761 ggtttcaacc ctttttgctg catctgctgc gctaatttct ttggtccttt gtcacttaag
     2821 cgcatattcg tgcgcacata actttcccca tagaccaaat catcgacaag gtccaattct
     2881 tttaagcggg ctacgatttt atttcgatct tcttgaagga tttccttttc ttttaagtat
     2941 gttcggattt ctttttccga tcgcaactga taacttaagt aattcattgc taggtgaaaa
     3001 ccgaaatcag ctccgctact cttcttgatc tcttggatag tttcttcgtc taattcccgt
     3061 ccttttaata atcgatagcg tactaataca tcttcagata cttgtaaagc ttctccttca
     3121 gataaatcta cgcgatagaa agggcctttg cctttactga ttctcgtaac ggttaacata
     3181 aacatcctcc tcgaacatat attctctatc ataccacaga aaatccccgt aatcaggtat
     3241 tatatgttag aataaaccac agaaaaaaga aaaagggaga tacttatggc agaaacaatt
     3301 gtcaaaactg gacaaacggt tcctttgaaa atcaagcgtt taggcataaa tggtgaagga
     3361 atcggttatt tcaaacggtt gatcatattt gttccatatg cactaccaaa agaagaagta
     3421 ctagtgaaaa tcacgaaagc aactcctcgc tatgcagaag gagaattaat aaaagtcaaa
     3481 aaaacaagca aagatcgcgt agtggcacca tgtcctgttt actatgaatg cggtggctgt
     3541 caattacaac acttagctta tcaagctcaa ttggatttta aaaaggatct gcttttgcaa
     3601 gcattggaaa aattcaaacc agcaggcttt cgatcttatc aacttttgcc gacgatcggg
     3661 atggatgagc cttggcatta tagaaacaaa gcacaatttc agttacgaaa aaataagcag
     3721 actcaaaaaa ttgaagctgg tttatacgaa gcaaactctc atcagttagt ccctataaca
     3781 gactgcttgg ttcaagaacc tcgaacacaa aaagtaatga atactgttgt tcaactattg
     3841 aataaatttg atgcacccat ctatgatgaa cggacaaaca gtggaatctt ccgcacaatc
     3901 atggttagaa tcggtgttaa aactggggag ttacaagttg tttttattac tcgcagtaaa
     3961 aaattcccgc aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc cgaagttgtt
     4021 tcaatcatgc aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga tgaaacgatc
     4081 cacttatggg gaaaagaagc cattgaagaa caaatcaatg aagtgacttt cgatctttct
     4141 cctcgtgcct tctttcaact caatcctcag cagaccgaat tgctttatgg cgaaggaatc
     4201 aaagcattag atgtccagcc taatgaaacg atcgtggatg cttattgcgg agttgggacg
     4261 atcggcttaa gtgtagcaaa acaagccaaa gaagtacggg gaatggatac cgttcctcaa
     4321 gcaatcgctg atgcaaaaaa aaatgcggaa agattaggat atcacaacac tcgatatgaa
     4381 gtcgggacag cagagcaagt tctgcctaaa tggttgaatg aaggttttca gcctgatggt
     4441 atcatcgtag atccaccacg tacgggtttg gataacgcat tgatccaatc attattgaaa
     4501 taccctccaa aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc cagagatctg
     4561 gttaatttaa gtaaaaagtt caagattgaa tatttacagt ccgtcgacat gtttcctcag
     4621 actgcacgtt gtgaagttgt cgtgaagatg acgagactcg ctgactaaat gaaagaggtt
     4681 gtgaaaaagt ctgccctgta tcaagtaata agaacagcca aacaaaaata gctcttcatg
     4741 ttttatgaat actgtacttg tgaccgaaga gttaacagga ggacactatt tatttaaaaa
     4801 aagggttgtg acatagtttt tgtcacagcc ctttttgatt attattggat attcacgtct
     4861 atcacttgat aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa aatcacatta
     4921 tatccttttc gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg tggaacagtt
     4981 gctttgtcgt ctatttttgt gatcaattca aaatcactaa atttcaaagg ttgattagga
     5041 ttccaagttg gtttggtaat gtagtaatcc caagtacttg tccgatgttg tgctgttaaa
     5101 ttccaagtaa tatttaatgt tcctggatta accatgtttt tatgccatct gttagatgtc
     5161 tgttcatcta aaggctcaaa gccgctgact cctgcactag caatttttcc atcaatgaat
     5221 gtatttttgg tagcttcgat actttgcggt tcccattgtg ctcttccgac attttgattt
     5281 aagtttcctg ctgctgatgt tcctaggtat gcacggcttc ctggagaagt aatataacca
     5341 tgcgcaaaac ctggaatgct aaaaccgaac aaccctaaac taactaaccc aataaacaat
     5401 aacgtactaa tctttttcat gtagaaacct ccttcatttg ataaccacat tgtaaacgct
     5461 ttattgtatt aacacaaaca ttatgctttt ataattttat gttgaagtaa aaaaggtgaa
     5521 aaaagtaaga tgcagattat aacagaagta gacgagaaaa gtacaaatag aaaacatgag
     5581 aatcaaatca tatgatcttt ttcaaaaaca tcattcatat aaacaaatgt tcaacttttt
     5641 aataacaaca attatcaatt tttttcttac ttcacaaact aaatacaggt cttgctgtga
     5701 gccttcatgg taaactaata ttatagaaag gagttagtaa aatgaagatg ataaaagata
     5761 ttcgtttttg ggtttgtgtt ataggaattg tcatattagg ctttttatca gggttgctta
     5821 gcggaaatcc cggaaaatat tactattctt tgcaacttcc accatttgca ccacctagct
     5881 ggatatttgg gccgatgtgg accttactgt atatattgat gggtatttct ctttatttgt
     5941 tacttaatca caacaacaaa aaacagcgaa acaatcttgt cggtcttttt gtgattcagt
     6001 tcatttttaa ttttatatgg tctgctctat tttttaattt aagaaatatt ttcattgcag
     6061 caatcgatat tacactttta gttattttct tgtcagtgct tatgtatcag ttgtggctgc
     6121 atcatcgcct ggcaatgtgg ctcatgattc cttattattt atgggtacta ttcgcaactc
     6181 tattgaatta ttcaatttat tttttaaact aagcgctaaa aagagacgtc ctgtaattta
     6241 acacatagaa aaagtgccac ctaagttaga ttttttgtct aatttaggtg gcactcttta
     6301 ttaaccaaac tatttttttc tagtacccac ttgttgttcc atactcatta ttctggtcat
     6361 aaccataacc attttgttcc tgtccagcgt aaggatcata actttgagta tcttgacttg
     6421 gtgtatagta ctgacttgta tcttctgttt gctgataata ggtattcgga tcttggtact
     6481 gatcatatgt gttttctgaa taggaagaat aatcggtgta cccttcacta ttcgatccat
     6541 caccgtttgt cgaatcgcca tcttccgaat catcaaagaa catcattgaa gcattctcat
     6601 ttttcaaatt aggtaatgta tcgcttgttt ttatatttaa ttgtttcttc agttcatttt
     6661 ggatcgaaag aagatcattt tttcctaaaa tttgatagta gatatcattg atcatttgtc
     6721 cttcgccttg caactggtct tgtttgattg tttcaaacgc cggggtgtag ttcgtcgcta
     6781 catcaagcat gtcatcccaa tctaagtccg tcgtcacatt gttctcaact gcttttaaaa
     6841 tttttctata attgcttacg ccatctaaac ttaaaacttt attgacgatt ttcgtcacta
     6901 cttcccgctg acgacgttgt cgaccgatat cgccttctgg atcttcgtgt cgcattcgag
     6961 aataggctaa acctttttct ccattcaatg tttgttcacc tactggtaca tggacaccat
     7021 ccaaagtaaa gtcaatttta ttattcactt tgatgccacc gaccgcattg atcaaatctt
     7081 ccatgccatc caaattgatc gttacataat gatcaatagg aatatcgagc agttgctcaa
     7141 ttgaatccat ggccatttcg acaccaccaa atgcgtaggc atgatttaat ttatctaccg
     7201 ttccataacc tacgatattc gtataaatat ctcggtccaa actgatgatc gttgattttt
     7261 tttgtttcgg attaactgtt actaccatca tcgtatcaga acgtccttgt tccgttcttc
     7321 ctaatccacc ggtatctaaa cctaatagca aaaccgaaaa aggttctttg tcatcaatgc
     7381 ttactctctc agaacgtttg gaggatattc gattagagtt tttagaaatt ttgtttactg
     7441 tttggctcgc ttctcccata aatctgattc cgtatgctga taatccggca atcagcaaaa
     7501 cgaatacccc taacaaggtt aatagaactt tttgccatcg cttcataata cacctctgtt
     7561 tcacattttc taaaatattg tctcatataa ctgttcaatc agcaaattca ctttttaatc
     7621 ataagaaaac tttaaaaaac atttttgtgt aacgattatt attttttaaa atttctcaaa
     7681 attcttaact atcataccat accgtgaaaa agatcgaaag accgaataca cgcagttcgc
     7741 aatcgtttta tcttttttta tgtatttttt ccagaaaaag cgaacaacaa ggtattttcc
     7801 aaataatcgt gcagtaaaat gatcgcacat acctttccca tctttttaac agtctttgcc
     7861 taccgttcag attatcattc gatttcacga caatttcttc ctatgactgg tccttttgtt
     7921 aaacccatct tctattcgtt ttatttccag ctcactgcat ggctcttaaa atgactcgaa
     7981 ctagtgattg cattaccatc atcttttaat ggatagatat gctcctcttc aaagtaaaac
     8041 gattttcttt taaagtaata gaattgacat ggtaatgagt atgtatacta gtggtttcac
     8101 aaatggtctt ttattcaaca tccaaaaact gaaatttctt taaaatccat tactaacgaa
     8161 gaaaatgttc cttgcttgat aattagttag gacatgtcaa gtgaacaaat gcatttactt
     8221 agccaaaaaa tctaaaaact tcataaatag acaaaaagac acgactctct aagaagtcgt
     8281 gtctttattc aacaaattat tataatttag tcacttcagc agcttgaggt ccgcgtgcac
     8341 cttcaacaat cgtgaattct acttcttgac cttcttctag gctcttgaag ccgtctcctt
     8401 gaattgctga gaaatgaaca aatacgtcat ctccgccgtc tactgtaata aacccaaatc
     8461 ctttttcatt gttgaaccat ttaaccgttc cgtgttccat aaatgtatat cctccaaaaa
     8521 agttcttgca agtaaccttg caagaacatt atacccaatt tagaaaacgc tagcaagctt
     8581 tcgctctttt attttccaaa ttatttttaa gactgatatg acggaggaaa tggttatttt
     8641 gctgcttcga aacgtttgtt tacttcatcc caattaataa cattccagaa tgctgcaata
     8701 taatctggtc ggacattttt atatttcaaa taataagcat gttcccaaac gtccaaacct
     8761 agaattggtg tctttccttc catcaatgga gaatcttgat ttgcagtaga ggtaatagct
     8821 aattttccat tttccattac aagccaagcc catccagaac caaatcgtcc agcggctgct
     8881 tttttgaatt cttctttaaa agaagaaaaa tcaccaaaag cttcattaat cgcttctttt
     8941 atttctcctg taggttcgcc accagcattt ggtgccatga tttcccagaa aaatgaatgg
     9001 ttagcatgtc cgccaccatt gttacgtaca gctgtcttga tatctgttgg aatagcgtcc
     9061 atatcagata ataattcttc tattgttttt tcgcctaatt ctgggtattt ctcaattgct
     9121 gaatttaaat tcgtcacata agtattgtga tgcttatcat gatgcagatg catcgtttct
     9181 tcgtcaatat aaggttccaa tgcgtcatat gcataaggta gatctggtaa tgtgtaagtc
     9241 ataaaaaaat tcctcctcaa tgttttctta ataatgtcta cattacaaag agtaacaaag
     9301 agaaggaaat tcttcaattg ttatgcctag tcgtcttttt cactttgacc tacttcttct
     9361 tcaaaaacct cagtttcttt aaaaataaac aacttgctga aaatatagtt cgcaaggata
     9421 acaataattt gagtgatgat tttcgccaca tactcattgc tgttcataac attgatcaac
     9481 aataccattg cccccatgtc caatccgtaa gaaaggatac ggtagaagaa gaacttaccg
     9541 cattcaataa gtgcatgtgt aaaattcggt gattttgaat ggaataccca aagtttgttc
     9601 gttacaaaag caaacaatac agaacaaaac cacgaaatag aattggataa ggccatactt
     9661 aaatcaaaat attggcgtgc gagagcaaaa ctaacaaaat ttacaatcgt agccaaacca
     9721 ccaaaaaaga gatagactag tatctcccaa tatccttttg cttctaaata tccttttaac
     9781 tgtcgatact gcttcatgag ccaacctacc ttattaaaaa ttcttattta tcatactcta
     9841 tatttcaata actttcatct ttatttaaaa aaattaattt tttttgatcc tttgcctttg
     9901 tctctgcttg gatcgtcaca tgtgtaatac catattttaa ttttaataaa gaaattatac
     9961 gaggatatat cttttcaatc tcgcttagta gctgatcttc aaaatcaata tgtccgtcga
    10021 aaataattat cttttcgcta tactgccaaa tatggacatg atgaacatcc tttaccccat
    10081 cgattgcttc aatttcttgt tccatttttt catagtcgat agcaggtgca ctttgtatca
    10141 aaatagcggc agcttttttg atcaccatta tcgcttctat caatatatat atcgaaatga
    10201 tcagtgtaat aactggatcg atccagtaga tatcgaaaaa acggatcaaa attgctccga
    10261 taatcacact taccgaagac agtgtatcac ttaacaaatg caaataacta gattttatat
    10321 tcaaactttc atctgcttct tgtcgtaata ataatactga gaacaaattc gcgagcaatc
    10381 caataactgc tactgttagc atcaaatgac tatttatctt ttctggtgaa gtgaaccgct
    10441 tgaatgcttc aacggttaaa aacaaggata atatgatcaa tatgaatgag ttgatgaaag
    10501 cactcaatat ttctaaacgc tggtagccat acgtacgttt tttatttgct tcttttcggg
    10561 ccattttttg tgccccgtaa gaaaaaataa ttgccaatgt atcactaagg ttatggaaag
    10621 catctgaaac aagagacaaa ctccctgaca taatccctcc aataaattct gataaagtga
    10681 tcaccgcatt caaaaccact actccaaata gtttttttga tgatctctgt ggctgtgtag
    10741 gcactattcc ttcctccatt ttatttatat tggatagtct ttagacaaga ataacatttt
    10801 tttgatattt caccaatcat tcagctgttt ccttaaagca cttgtatttc acttgatact
    10861 tcgctagaat tgcaagtgat gacactacta gatgaggagt acttatgaca acaaaacaat
    10921 taattatcag ttcctttata cgattcaaag acctaaagga ggcgagaaat gtcccatttt
    10981 ggaaagccat tgtctatctt ttagttttga gtataattat ggctttgcca atctcttttc
    11041 aagtttttca agtcttaaac aacattcaat tggatgggca aaagattgct actcgcattc
    11101 ctgattttac aatccaaaac gggcagcttc aaaccgaaga aaaagaagga ttcatttacc
    11161 aaacaaactc cattatcttc acatttgatc cagagggaaa acgaacagaa caggacatct
    11221 ctactgattt gatggggaat tttgttagtg tcgggatgtt aaaaaacaaa cttatcatag
    11281 ctcttcctaa tacaggcacg acttctgctt tgttgaacaa taaccaatta gaacttccct
    11341 acaccaatga atctttaaaa aacttaacag gtaagcagct ccgttctttt ttaagtgaag
    11401 catctatacc actttggata aaagtcctga catttatatt ttctatttat cctagttttc
    11461 tgaatctgct gatcactttg ctatttacca acattgctgc ttttctctat gcacgctttc
    11521 gactaacaaa agctactttt cttgattgtt taaaaacatt gatctattca atttcattgc
    11581 ctactataat tgcaacaatc ttgatggtct ttcttccttc ttttgacact agcgccttca
    11641 ttgcttttgc aggaatcttt atttttgctc aagctgtcaa aggatgggca aaaatatcga
    11701 tcaattaaga aataaagaca actcgttatt tctacttcaa tagacccttt tagaggttgt
    11761 gacatttttt tgtcacagcc ccttttttat ttgaaaaata tttctttctc aaaaaacaag
    11821 caaggcgctt caaagttctt cttgtatgcg ctacacttag agtaaagaaa ggcgggaaat
    11881 taactatgtc agaaagaatc atttatgcag atcgtccatt tcgtgatgaa tttatcgaag
    11941 aaatccatac tatagcgcct ggttatatct ttaaaacaga attacaacca gaggatctac
    12001 aaaatgtaga gatcagtttg ggctggaact cagactacca agaaaaacta ttagcctctt
    12061 ctaccttgaa atgggtccaa tctatctcag ctggtgttga tcagctaccc cttgagactt
    12121 tttcaaagaa acatatttta ctttcaaatg gtagtggcat ccacagtgaa tcgattacgg
    12181 agcatgtctt aggaatcatc cttgggtatt ctcgtggtct ttttcaagca caaaaggcac
    12241 agagtgacat gaaatggtta ggttcatcgg tccattacca atctatccaa gggaaaaaaa
    12301 tactaatcat tggaactggc catattggaa aaatgattgc aaaaaaatgc aaagcattag
    12361 acatgaactg ctatggaatc aatacatccg gtcatccagc agaaggaatg gaaaagactt
    12421 tttctttaaa tcaactaaag gaagttcttc ctgaagtaga tttcgtagtc aatatcttac
    12481 ctctaacgga ggaaacaact gggctctttg atcagcatat gtttgaacac tttgatccaa
    12541 aatctgtatt catcaatgtt ggccgtggtg cttctgttaa gacacaagat ctggtgcagg
    12601 ctttaaataa ccagcaactt tcctttgcag cattagacgt ttttgaggaa gagcctcttc
    12661 cacaagatca tcctctttgg aaaatggaca atgtactgat tacttcccac attgcaggtt
    12721 tgacacctga ttttcaaaag aaattaatgg ctattttttt aaccaatctg aaaagctact
    12781 ttgccaccca tgagcttcaa accaatcaag taaaattgtc tgcaggatat taatagctga
    12841 aaacaatatt ttcaaaagaa aaaagagaag gtgacaaaaa ttgtgtcacc ctctcttttt
    12901 ttattgtttt ttaaagtgat aggttggctc ctccaggttg ggatcataat caacaagtaa
    12961 atcatatcct ttaaagaacc attcatctgc ttcctcgata aaaaaaagtg tcccatcaat
    13021 ttccactttc tttacaggat gttctggctg ttcaactgac attccaatcg aaaaaccatc
    13081 atgaacctct gttttgccgt agacttttcc aaaaaaacgt attccgtaag tttctggcag
    13141 atcaatttct gagtcaaacc acttttgtgc ttttggcgtt accgttaatt tcatcccaac
    13201 aacctacttt ctcttttctt tctattgatt catcttatcg tctagaatat ccacatcgcc
    13261 tgtctctcca ataaccagta gattatcatt ttcacgaaca tattcatctg ctgcaggaga
    13321 agcgataacc gtttgatcta ctcttcgtat ggcgactact gttaagtcaa aacgttgcct
    13381 gaaatttaat tcagctaatg ttttgttgaa aaatttagga ttggagactc ggatttctgc
    13441 taaagaatat ttatctgaca actctaggta atctaaaata ttctttgaca ctaaattatg
    13501 cgcaatcctt attcccatgt ctcgctcggg atggacgacg cgatcagctc cgatcttttc
    13561 aagtactctg gcatgatact cattttgggc ttttgctaaa atatttggta cgcccatttc
    13621 ctttaccatc agagtaacta agatactggc ttgaatatct tctccaatag cgacaattac
    13681 atgatcgaaa tttcttatgc ctaatgatcg aagtgtcatt tcatcctgtg catttgccac
    13741 aacagcatga gtggctatat tcatatattc attgacccga tcttcattac tatcaatagc
    13801 cagtacttct tgaccagctt cgatcagtgt tcggcagata cttccgccaa atcgtccaag
    13861 acctacaatt gcaaaatttt gtttcaccgt tttcctcctt tagtatcttc ttttctcagt
    13921 ataacaaagc ctttacaaaa acaaaagcaa gcatcattat tgtaaaaaag ccaagaaaaa
    13981 taatagttat tttctcagct tttgattatc ctctttatca tcgtttttat ttcaccaaac
    14041 catgtttgaa tgcatagata gcggcttgtg tacggtcttc cacttctagc ttagccaata
    14101 tattggaaac atgtgttttt actgttttca gtgtaataaa caattcgtcc gctatttcct
    14161 gattactttt tccttcagaa atcagcatta aaatttcatt ttcccgattc gttagttctt
    14221 catgtaatac aggctcattt cgccgactca ttttttccat catttttgtt gtcacttctg
    14281 gttctaatac tcgctcgcca cgttgcgttg cccttatagc atcagcaatt tcatgtgcgg
    14341 tagaggtctt caacaaatac cctgctgcac ctgcttcaat ggcaggataa actttttcgt
    14401 catcaataaa actggttacg ataatgattt tagcttctgg ccaatctttt aagattgctt
    14461 ttgttgattc gataccatcc atttcttcca tgactaagtc cattaaaatg acgtccggtc
    14521 gaagtgtcat cgccttctca tagccttgtc gtccattttc agcttctcca attacttcaa
    14581 tgtcttcttg aatagataaa taagaagaga cgcctaagcg caccatttca tggtcatcta
    14641 ctaataaaac ttttatcatc cttatctatg cctccttcat caaagggacc ttgatttcta
    14701 cacttgtacc ttgaccttta aaactaatga tttttactgt tccaccaata ccagctactc
    14761 gttctttgat attgttcaat ccataacttc ccgttttcgt ttcattcata ttaaaccctg
    14821 ttccgtcgtc aataatacgt aacaaaagat tattgtctat tttgtgcaaa tatacctcta
    14881 attcattggc tttagcatgt cttaatgtat ttgataacaa ttcttgaacg attcggaaca
    14941 agtgatcctc aatggaacta gatagtttta catcttctac atcccatttc agcgaaatct
    15001 ggattttatt ttgcaattcc ttcaataact gctcgatacc ttgctttaaa cttttctctt
    15061 ctaaattgac tggacgtaga tgaagaagca gcgcacgcat ctcagactgg gaagcgttga
    15121 tgatctctgc taccattttc aactgcttgc gaaacatttc tggtgtctcg cttttttctg
    15181 cttgctccgt caatgccgac atcatcatca ttgctgcaaa caattgttgc gaaactgaat
    15241 catgcagctc acgggctaat cgatgtcttt ctaattctaa aatttcttct ttggtttgtc
    15301 catctacata ttggggacgg ctcgttacaa tttgtaattc actagatact tcaatcattt
    15361 tttcttttat cttagtgatt tccttatcaa tatctttgat gtataaatcg tccgatgcac
    15421 ttgggatcgg aagaagcaac aatttggatt cataattgcc agaagaaagt gcacgcagct
    15481 tttcttcgat tttcccgtat tttgtctttt gaataagtga taataataga aagacaatta
    15541 atcctacacc taaggaaatg gctaacaaat ggaagataag cggaacatat aataaacgtg
    15601 cacgcagtaa ttctccccac caatgacttt gattgctggc ataaaaataa gtgaaaagtg
    15661 aaaatagaat aataaggaaa gccgcaatcc ccgaataaac agctagcatc gctctggata
    15721 tttttcccat catattcgta tcacctcgac atctccaatc aaggtattag taataatctt
    15781 caatctacga gggttttcat cataatcact gctgtagatt ttgatttgtt catttttcaa
    15841 agaaaattgc tcttcttcaa acaagacatt tcctactaat gtcgcatgct ctaatcggat
    15901 ggctacgccc aaaggtacta agatccgtgt tctgcctatc ccttttctta caatcacaat
    15961 attatcatct tttggcaaaa gcgtattgcc caaatctatg atagtatcac cagaaatgac
    16021 ggcgatgttg atatcgtccc attcatagac gtcgtttcca atacgttggt taccgaataa
    16081 ttgctgtctt ttccgctcat tattatgtgt ttttaattgc tctgtttgga tcatcatgat
    16141 ctgtttcttt cgccaaaaag catttttggt taaatctatc cctgaaatct ctacaccttt
    16201 cagaccaata aataaaacgg caaatacagc catcatccat aaagcaggac tgttgactaa
    16261 gctaaaaaaa atgaccaaac ttcctaatat caattgaaaa ttttgaaatt tagtacgagg
    16321 atgtttgcgc atagctagat atatattgaa aataccgaat atcactaaaa gcaaaagtcc
    16381 agtattgttt acgatttgcc aaacagcaaa taacagcagt agtgcttcaa ctaccacaaa
    16441 aaaacgccaa gaactattca tcgctctttc tccctcctga cacattctca tttcatcata
    16501 cctatttttt taactgttgg ctataggact ttagatgtaa gatcgtttgt tgaaatctaa
    16561 aatacacaaa gagaaaaaga tctagagtgt atcatcagta agatgaactc tctagatctt
    16621 tcagtcagac tacgcttgtt ctactttgac gattttaact aacatatcgc cacctggagt
    16681 agcaatagtt acttcatcat ttttacgttt gccaatcaat gcttgcgcaa taggagaatc
    16741 attggaaatt ttgccagaaa acggatctgc ttcagcgctt ccgacaatcg tgtactcttc
    16801 ttcttctcca tctggtagtt cgatgaatgt aactgtttta ccaattgata cctcatctgc
    16861 atctacaccg tcgttatcga tgatttgagc aaaacgaatc atattttcaa ttgttgtgat
    16921 tcttccttca acaaatgctt gttcatcttt cgctgattca tattcagagt tttcggaaag
    16981 atctccgaaa ccgcgggcaa ttttgattcg ttcaatgatt tctccgcgtt tgactgtttt
    17041 tagttcttct aattcttttt ctaatttttc tttaccttct agagtcattg gataaacttt
    17101 ctctaccatg tgtaacgtct ccttttttcg ataaaatacc gcaaaaggta atcttttgag
    17161 gaatatagtc caaaaaagat taccacgctt ttcttcaaat gtaaatgttt ttctaaaaat
    17221 gatcaatgtc tagagcctgt ttttctaaga attatttaca tatttttcca ctaattccat
    17281 atgttcttca taagttttag cgaaataaac atttccagtg ctcgtatctg ctacgaaata
    17341 ataatagtca ttttctgctg gttctaatac tgccttgatt gcctcttcgc ttggattgtc
    17401 aaaaggtcca ggtccatagc cggtattggt atacaaatta tatggtgaat ctacagctgt
    17461 atcttcatac gtcactaatt ctttgtgttc acctaatgcg tataaaacag agatatctga
    17521 ctgaagtggc atatttgctt tgattcggtt aaagaagact tgtgcaatgt ttttacgatc
    17581 attttctttt acgccttctt tttcaactag agaagctaga gttaaaactt cttgtacagt
    17641 caaatttttc tgcttgatag ctgaataata gttttgcatg accgtattcg tcttgttcac
    17701 catttcaatc actagatcct taagcgaagt actcgtgtaa taatcatatg tggctgggaa
    17761 taagtaacct tccaaacggt attttgtgtt ctcagcttta gatgcacttt ccaacagttc
    17821 tgggaatttt tggtgcaact cgttaaagaa agtctcatca ttcactaaat ctaaaaattc
    17881 tttcttgtct ttaccagtta ccttcgctac ccgttcagca atctgatcga tatcgtaccc
    17941 ttctggaatg gcaattttcc catctgcaac ttttgttggc tcagaagtac cgccttcttg
    18001 caactgtttg ccaatctcgt caagagtcat attaggcgct aactgataat agccagcttg
    18061 aaagccagtt aaatttttaa attttgtata ataattgaaa acaatcccgc ttttaataat
    18121 attgtccttc tccaaaatct caccaatctg cttgttagaa gagccactcg ggatctcgac
    18181 ttggactaat tggtcatctg atttatctaa aggcttcaac ccgctatcta cataacgata
    18241 cacggtaaat ccaagaaatc cgccaataat caataatgta agagcaatca ctaaaatgat
    18301 cttcctcaca atcttatctt cttttttacg cagatttctc cgcttgtcga ttatatctgc
    18361 tgtagctgcc ttactggctt cttttttatc tttacttggg tgttgtttgt ttcctgacaa
    18421 aacaactcct ccttactatc aagttcgtaa ctttattcat tatacatgat atttgaattg
    18481 gcgtgaatga ttttatttaa atattctatc aaaaatgtaa gtagtaaatc cattactata
    18541 agattaaaat aaatgttatc aagatacaag ctattttagc tttattcttt ctgtaaaaaa
    18601 gaaaggagac taaataaact attatgaaaa aaggaaaaca tgctaaaaaa agaaaatatt
    18661 catttttaat gatcgttgtc gtgttgttta gccttagtgc gttctctaca ggaactattc
    18721 ttggatggca attttacaat aatgaacact ctcagatagt tgcccatgct caatcagctc
    18781 aaaaaaagtt gatactgact accaatgaac aacagctcgt tgaaaaaatc gatcaactat
    18841 taagaaaaaa tgattatatt ggctcaattt acgtaaagaa aaataatcgc atcatcatgg
    18901 aaaaaggcta tggctatgcg aataaactca cgcaacaacc aaatgatcct tctctttatt
    18961 atcagattgg ttcggttcaa aaagcattga ctactctttt gattttaaaa caagtcgagc
    19021 gtgggcacct ttctttagat actaagctgg ctcaattcta tccacaaatt gctggcagcc
    19081 agcaaattac tatccgtgac ttgctgtata tgcgctcagg cttgcaccga actgcttctc
    19141 caaccatacc gatgactgat gaagaagtcg ttcaatttgc gattcagcac ttaaaattta
    19201 cagattatca gacctctcat tatgattcat tgaactttac catattgact ggaattttga
    19261 tcaaattaac aaatcaaccg tatgaaaaat tattgaccga tgatatcatc gaacctttag
    19321 gattacagca tacaaaattt tatgaccaag tcaaaaattc atccaatcat gcactatcct
    19381 atcaaatgtc taaagaggat gattacttta aaccactgac agaatctgaa acagagatcc
    19441 ataacgaatt agggactggt aatatcagta tgtccgtcta tgatttaaat acgctgttta
    19501 cacaagtttt atctggaaaa ctagtttcca aagacttact cttctcatta tggcgtgaaa
    19561 atgcgaatgg acatccatat agtggtgggg tatatagcgg agataattat atcctgtcgc
    19621 aaggaaatat cgactctttt gatacatctg ctatatttag caaagattca caggatgcta
    19681 tcatcatgga aagcaatata aaagctgaca aaactatcaa actgcctact gctgatctaa
    19741 gaaaccaaat ctatgacctg atggaaggaa ctactaagtt ggcaaatagc tgatatgatg
    19801 aaaagatagc aaatactact tctttgaata gcatttgctt aaaaaaagta ctcgactaaa
    19861 cgtaggaaag agtgtgggac aaatctaaaa atcaattttt gctacagact ttaattccaa
    19921 ataaacagaa cagaaagagg tgttcttctt tctgttctgt ccaaattatt ctaagatttc
    19981 tctttaccaa cattatttga caaagggcct attaaatcta aaatagtatc tttagattgc
    20041 taaaatatgt gctagcaaat cattcatggc ttgttcttat ttttaatttt atgcaataaa
    20101 aaagaagaac aattagcgtg attatagaaa taaataatga gattttagaa aaaaatgtgt
    20161 atgaatacct gattgttact ttatttattc cactttcact ttttactgta acaaaaggac
    20221 ctccttgttt tagtactgta gcattttttg aattaacagt aaccgttaat cctttatacc
    20281 ggaatacagg taaaactatt gttttgcctt ttgaagattt aaacgtaaat gtaatttcat
    20341 ctttatttgg agaaaagcta ctataaataa cttgaccatc ttctgtaact tgatggtttt
    20401 tcaaaagcaa ttgatctttt ttatagccta taggtacata atcaaaattc tcaaattctt
    20461 caagtttatt ttccatttgt tcattagaat aagttccttg aaaattcatt tctttcacca
    20521 tgttaactac tgatgaataa tttaaaattg aaatagctaa tattaataat agtataaaat
    20581 ttcttctcct tgtataagaa aatttttcca actgtggaga aataaataga gatactgaaa
    20641 aagatatgaa caatgttgaa aatatcataa aacgccaagg agcttgaata atatttaaag
    20701 gagtattttg taaggcaaac catggaaata tttttgtaca aagtagaata gatattatac
    20761 ccattatata agtgatttta gataagattc ctaatttttt aaagttaaat gttagtataa
    20821 ttaaacaaat aagcaaagtt aagccaatac caaatgatag taattctgaa tctatacttc
    20881 caatcaaaaa ctctttaccc agataaattg ctcttttttg taatatatcc acataaggtc
    20941 tattcagatc ttgatacaat gtctgctcaa tcataggacc tataaacgct agtgaaagaa
    21001 gtaaagtaac tatagcagct tttattaatt taataattaa gaagatttct attttctttt
    21061 tccgataaaa ttgaataatt aaagcaatta taatcattac catagtcata gctacagaaa
    21121 gaacatgagt gtaaattaat aaagtcatgc taatcgccaa cataacccaa gattcttttt
    21181 ttgagtcgta aagctttata aacccgagta aaataagagg aagtattgac attgcgagta
    21241 attcccccat tgcccctcga agaaatattt ctacactcct aaaagaagat gttgtatata
    21301 taatagcgaa aaaggagcta gtaacatgtt ttttggtaat ttcatacatt acataatgac
    21361 atataaatag agtaatgagc gtaacaatat aaagaaaaag gttatagcct attgctaaat
    21421 tagtaaactt gatcaataaa aataaaggat atagagttaa ccatgggtaa aaattattta
    21481 cttgcatgcc atatgatcta aacgtttcaa aattaatagg ggagctaaaa atagattcta
    21541 aacttaacgc acgattaatg tgaaatgccg tatcataaaa gccaaatatt tccatgtttt
    21601 taaatgctgg aaataaatat aataaactaa caaatgcata aactataaat atttttacag
    21661 aaaaa
//
LOCUS       DAHUVV010000042        21482 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.042, whole genome shotgun sequence.
ACCESSION   DAHUVV010000042 DAHUVV010000000
VERSION     DAHUVV010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.042"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            60..638
                     /locus_tag="NK767_001547"
     CDS             60..638
                     /locus_tag="NK767_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCH6694546.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            767..1144
                     /locus_tag="NK767_001548"
     CDS             767..1144
                     /locus_tag="NK767_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCH6694547.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            1077..3149
                     /locus_tag="NK767_001549"
     CDS             1077..3149
                     /locus_tag="NK767_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCH6694548.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(3360..4307)
                     /locus_tag="NK767_001550"
     CDS             complement(3360..4307)
                     /locus_tag="NK767_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCH6694549.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            4553..5017
                     /locus_tag="NK767_001551"
     CDS             4553..5017
                     /locus_tag="NK767_001551"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCH6694550.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(5079..6122)
                     /locus_tag="NK767_001552"
     CDS             complement(5079..6122)
                     /locus_tag="NK767_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HCH6694551.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(6499..7464)
                     /locus_tag="NK767_001553"
     CDS             complement(6499..7464)
                     /locus_tag="NK767_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCH6694552.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            7698..8543
                     /locus_tag="NK767_001554"
     CDS             7698..8543
                     /locus_tag="NK767_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCH6694553.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            8734..9921
                     /locus_tag="NK767_001555"
     CDS             8734..9921
                     /locus_tag="NK767_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCH6694554.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            9927..10283
                     /locus_tag="NK767_001556"
     CDS             9927..10283
                     /locus_tag="NK767_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCH6694555.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            10285..10623
                     /locus_tag="NK767_001557"
     CDS             10285..10623
                     /locus_tag="NK767_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCH6694556.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    10699..10955
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            11018..12052
                     /locus_tag="NK767_001558"
     CDS             11018..12052
                     /locus_tag="NK767_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6694557.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            12045..12731
                     /locus_tag="NK767_001559"
     CDS             12045..12731
                     /locus_tag="NK767_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6694558.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            12755..13603
                     /locus_tag="NK767_001560"
     CDS             12755..13603
                     /locus_tag="NK767_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCH6694559.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            13800..14573
                     /gene="sufC"
                     /locus_tag="NK767_001561"
     CDS             13800..14573
                     /gene="sufC"
                     /locus_tag="NK767_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCH6694560.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            14586..15872
                     /gene="sufD"
                     /locus_tag="NK767_001562"
     CDS             14586..15872
                     /gene="sufD"
                     /locus_tag="NK767_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCH6694561.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            15869..17104
                     /locus_tag="NK767_001563"
     CDS             15869..17104
                     /locus_tag="NK767_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCH6694562.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            17091..17561
                     /locus_tag="NK767_001564"
     CDS             17091..17561
                     /locus_tag="NK767_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCH6694563.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            17566..18957
                     /gene="sufB"
                     /locus_tag="NK767_001565"
     CDS             17566..18957
                     /gene="sufB"
                     /locus_tag="NK767_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCH6694564.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(19052..20194)
                     /locus_tag="NK767_001566"
     CDS             complement(19052..20194)
                     /locus_tag="NK767_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCH6694565.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(20233..20967)
                     /locus_tag="NK767_001567"
     CDS             complement(20233..20967)
                     /locus_tag="NK767_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6694566.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            21199..21474
                     /locus_tag="NK767_001568"
     CDS             21199..21474
                     /locus_tag="NK767_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6694567.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
BASE COUNT         7123 a         3995 c         4473 g         5891 t
ORIGIN      
        1 aaagtctctt ttttctcttt ctttttcgtg gtaaactgaa agagtgaagg atttatttta
       61 tgagaggaaa catggaaatg aaaaaaagaa atctgtctca agagaaaatc atcgactgct
      121 ttcgagaact agcagaagag atggatgttc agcaaataac ctttcagcat ttagccaaac
      181 gcttggatat caaggctccc tcgctgtata accatttcaa aaatattcgt gatgtcaaaa
      241 cagcgctaac agcgaaactt ctgcaggaac ttaatgacca gctgcgacgg gctcttgtgg
      301 gaaaaagtgg tcaggaagca ctgcagatct atgcagaaac ttatcgatcc tttgcttttt
      361 caaatcaagc agtgtatgag ttactgatca gtgtaccgca taccaatgaa gctgttttat
      421 tagatggaat ccatgaaacg aatcagatca ttttgcagat atttgaggct tttcaattaa
      481 cgaaagaaga aaaagttcat cgaagcaggg aacttcgtag tatgatccac ggttatcttt
      541 ccttacgttt tttaggatac tttacaaaag aaccaacaat cgatccagaa gacagctatt
      601 tttggatgat caataacttt atcgctactt tgccttaaac aatcagattc atttgtaaca
      661 attttaagat gttcattaaa tgaccctttt ataagagaaa attcttgcaa ctttcttgaa
      721 aagtgactaa gatgagtcta gaaaagctga aagaaggagc acgagtatgt atcaagtaat
      781 taccatgttc ggggacaatg agccttggtg gttttttgaa gactgggaag aagatattga
      841 agaagaagaa acattcgagt ctttcgaaga agcacgacaa gcgtacgaaa aacaatggaa
      901 cgaaatccat caaaattatg aatatatcaa tgcaaaaagt aactttctca gcgccttttg
      961 gaatgacgga gatgagcgct ggtgtgaaga gtgtgacgat gatttacagc aatacaaggg
     1021 acttgcgttg ttgaaaaatc atcagcccat cacagtagaa agcagaaagg aattttatga
     1081 aacaactaat tctagcggaa aaaccaagcg ttgcgaaaga cctaagcaag gtgcttggtg
     1141 ctaatcaaaa gcacaaaaat tattatgaag gacccaaggt gatcgttact tgggcccttg
     1201 gtcatttatt aggattgaaa atgccggaag atctgaataa ggaatggcag agctggcaaa
     1261 tggacacgtt gccaatgatc cctaaaaaac taggaatcaa accacttcct aaaacaggac
     1321 atcaattaaa agcaatcaag cagctggctc agcgaaaaga tgtctcagaa gcagtcattg
     1381 cgacagatgc cggaagagaa ggggaacttg ttgctcgctg gatcttagag tatgtgcatt
     1441 tcaataaacc agtgaagcgg ttatggatct cttctcaaac agataaagca atcaaaactg
     1501 gattcaagca actaagacct gcgaaagaat atgatgcatt gtatgaatct gctcttgccc
     1561 gggcaaaagc ggactggctg gtaggattga atgtgaccag agccttaact gtgaagtacg
     1621 cggataattt atcagcaggc cgtgtgcaga caccgacact agcattagtg agagaccaag
     1681 aaaagaaaat cgagcagttc cgtccacaaa cgtatttcac gatcacgttg caagctggaa
     1741 aagaacaagc caaaatgctt caaaaaaatc aatttgcttt aaaaacacaa gaagaagcac
     1801 aaaaacttgt tcaattgatc agtacaagca aaggaactgt cacagaagtc caagaaaaat
     1861 taaaaacgga atctgcgcca ttgccttatg atttaacaga gatccaaaga gaagccaatc
     1921 aacgctatgg ctattctgca aagaaaacat taagtcttgt acaaagtttg tatgagaccc
     1981 ataagatcgt tacttatccg cgtacagaca gcaaatatct gacaaatgac atgaaaagta
     2041 cgatgaaaga acgattgcaa gcagtcagcg actttgctcc tgaggtcaag cagtatttga
     2101 aaaacggagc catcgtccaa cagacaaaag tattccaaga cagtaaagtg accgatcacc
     2161 atgcactttt gccaactgaa aacagagcac gttatgaaaa attaagtaat gaagagcgaa
     2221 aaatctatca aatgattgtc tctcgttttt taggactgtt tgctcaaccc cataaagtca
     2281 gccaaacaaa agttacggtt gaatttggta aagaacgatt catttttcgg caaaatcgtg
     2341 tcgtccaagc aggatggaaa ggcgaaactg aaactgagac ggaaacagtc aaatgggaaa
     2401 aaggaatgag aatcaatccc gatttcgcca tcaaaaaaga actatctgca ccgcctaaac
     2461 cattgtcaga agcaagcttg cttggtcaaa tggaaaaata cagtctggga accccagcta
     2521 cgcgtgcaga gattatcgag aaactaatca aatctgaact gatggaacgt acccctcaag
     2581 gactgcaagt atctcctaaa ggtaagcaat tattagtatt ggtaaatccc tctttagtaa
     2641 caccagattt gacagaaagc tgggaaaaag accttgaagc tattgctgca ggaaagaaac
     2701 aagcaggaac cttcttgaat ggaatcgaaa aagaaacaaa acgcttggtg aatgagatca
     2761 aatcaagcaa acaagaatat caagatttct cgattaccca aaagaaatgc ccagaatgtg
     2821 gggccaatct gcgcgagaaa aacacacgtg acggcaaaat ctatgtctgc accaattcag
     2881 attgcaacta ccgcagaaga aaagatccaa aagtctctaa tcatcgatgc ccgcaatgtc
     2941 accggaaaat ggaaatcatc gaaggtaaaa acggtgctta tttccgttgt aaatacgatg
     3001 gaacgacaga aaaattgctt gataaaaaag agcagaagaa gaaaatgacc aaacatgaag
     3061 aacgacgctt gctgaaaaaa tatgcacaag cagaagaacc agaagaaagc ccattagcag
     3121 cagcactaaa agcagcaatg aagaagtaat aagatatgaa agaggttgtg atagaagtgg
     3181 tctgcttcaa gaaataagaa ggaatttacg aaaattgctt ctcaaatttt tgtgaaattt
     3241 cggcttattt ccgaagaagc tacttctgga acaccattta ttcggaaaaa aggactgtga
     3301 caagactttt gtcacagtcc tttttcattt aaaaaaatgt ttacttcatt tgacgtatat
     3361 tattggtgag gcttcaattc atttggcttc actttttctg tttctcttat gatataaaca
     3421 ggacgctgtt tcgtttctaa gaagattttc ccaatatatt tcccgatgat tccaagacat
     3481 aacaattgga ttccaccaat gaacaggaag atcgagacca tggaaggcca tcctgatgta
     3541 ggatcaccaa acaacaatgc ccgcaaaata atgaacaaaa ttgcaatacc agaaccgaca
     3601 caagacaaag cgccgataaa tgaagcaatc gttaacggtg catccgagaa gttcacgata
     3661 ccatcaatcg agtagttgaa taatttccag aaggaccaag atgtttcgcc agccacacgt
     3721 tcgcggtttt cataagaaat atattctgtt ttaaatccga cccagctaaa taatcctttt
     3781 gaaaatcggt tgtattctgt taattctagg acggcatcca ccatttgacg agtcatcaag
     3841 cggaaatcac gagcgccatc gaccatttct gtatcgctga ttttattgat caactggtag
     3901 aattttttcg caaagaaact gcgtatcgca ggctcgcctt cacgagtcga acgccgggtg
     3961 ccgacacaat cgatatcaga agtagtgatc cggttgatca tctcaggaag cagttcgggc
     4021 ggatcttgca gatcaacatc catcacggtc actaaatctc ctgtagaagc ttgcaatcca
     4081 gcgtacagag ccgcttcttt cccaaaattt ctggaaaatg agacaaagcg gacataagga
     4141 taagctgagg ctagcttctt gattgctgct aacgtaccgt cttttgatcc gtcgtcaata
     4201 aacaaatatt cgaactcatg ggcacctttg aatttttcaa cctcatcaaa aaaaagagga
     4261 acagtttttt cttcattaaa acatgggaca acaattgata ctttcatcgt ttctatcctt
     4321 tcaatcgtat gtttatatat ggtttacatt caaaatttaa taaaccttaa ataacacctt
     4381 taaaatttga ttttacgtaa aagaacaggt attctttttg taaactaacg tatttagttt
     4441 accatgtttc ctcggtttat caaactttta agaacaattt ttattagttg gcaagtagtt
     4501 ttggatagag aaaaaggagg aagttccagt ctattgacta ggagcaaaca aaatgaaaat
     4561 acacacacct atcggctttt tatggttaga tgcagatgaa tccggattga ctgctgtatc
     4621 ttttcagcca atttcagaaa gcgaatcagc gaatcagcga attttaacgc gcacacaaga
     4681 acagtttgaa gaatattttg cgggaaaaag aaaagaattt gatgttcctt tatctataaa
     4741 gagaggtacg ccttttcagc aaaatgtctg gaaagctttg tcgaccattc cttatggaga
     4801 aatccgaacg tataaagaag tagccgttgc tgttgatagt ccgaaagctg tgcgagcgat
     4861 tgggcaggca aatcgaacca atccattgcc aatcgttatt ccttgtcatc gagtgatcgg
     4921 gcaaaatgga aaactgacag gatatatggg aaacgcaata gatggggttg aaatcaaacg
     4981 ccaattgttg ttgttagaag gatatctgca aaattaaaaa cagttgtgat tcggacattg
     5041 ttccatgcga gaatcacaac tgttttttca tgttcattct aaactttgat cagccaaata
     5101 aatagtaaag ccatcactgt ttggagaagc ccgacagcta atccataaga taagaagcgt
     5161 gtaccttctt tcatgaattt tgcaaagtct aaacgcagac caattgccgc taaagcagtg
     5221 atctcaaacc agctgctgat aaaatgtgcc gattggtcaa taactacagg taaagaaaac
     5281 aagctgttga ccaaacaaaa gatcacaaac cctaatacat accaaggaag cggtaatttt
     5341 cgttttttct gcgtatcagt tggcgtttca aacagagctg gttgtttctt gtgcatgaat
     5401 ttttcaaaca gatagacaac aacaacgagc atcataatac gtagaatctt aaatagcatg
     5461 gcgaattcga caacagaatt gtcaagtaag cttgccccag cgactacttg accaacagat
     5521 tggagtgtac cacccaaaag tgcacttttt tcaatgagat ttccaccaaa caactgtaat
     5581 ccaagaaaag ggagagtcaa catcatcaca gtacccagta gattgactag tgtgatgatc
     5641 tgtccttttt cttcctcttt cgcatggatg gaaggggcga ttgaagcaat agcagaagaa
     5701 ccacaaacag cgtttcctcc agccatcatc aaagaagaag tatcgctgaa ttgcagtttt
     5761 ttaccaatca agtaagcacc gatgatagtg actgacattt gcagtaacac aaaaacaatt
     5821 ccttttacac caatttgagc aatcgtttga gctgtgacag ttgcacctag taaaaaaaca
     5881 gagaattcta atagtttgct ttcagcaaat tttgtgccga ttgctagaac aggctgttta
     5941 aaaaaagtat ttcctaataa aatacccaat aaaatagcaa tagtcgctcc acctaactgt
     6001 ggcagataaa tagcaagaag cctgctgaag gcagcgatgg caaaagaaag cagtaaaccg
     6061 ggtaagattt cgcggaccgc tgaatccttt acagatttta ttggatgtga caatgatttc
     6121 atttgatcgg ttcctttcgt caataaatac taaaagcata ctcgaacata gtagcactaa
     6181 accagtattt tgacaaaaga acaagaggtc gtgacaaaag tcttgtacga cctcttgttt
     6241 cgaataaacg gtgttccaga agtagcttct acggaaataa gccgaaattt cacaaaaatt
     6301 tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt cttttacgac
     6361 ctcttggtaa acggtgttca aaagcttgca tctgcggtaa taagcccaac cagtacaaaa
     6421 acatgaacag ctgtttttgt ttggccgttc ttattacttg atgcagaaca gctttttcac
     6481 aacctattga atcgattatt aagcgatttt tttagaaaca aattttccat gtttgctatg
     6541 ttctttaatt aagaaacgta cgtaatgagc aagaacgtag aagacaacta cacttgcaaa
     6601 cagacattcc gcaactttta agaaatccaa tacgctacta tagataccta aactgttgaa
     6661 gtaatgaaca gtagtaaata tagctgttgc agagataaca agcgggaatg taaaggctgc
     6721 atagcttgga taaaaacgaa gcttgaacaa acgtggaagc atagacagca caacgaaata
     6781 caatacttga gataaagcga acaatgctac gacaagataa aaattaggtt cagcaaagtt
     6841 tttaagataa cctgttaggc aaagagaacc aggtgctgca acgatcgtaa tcagcggacg
     6901 tgtcggatga gccatatttc ttacgaaaaa gacacgtttg atgatgatcg gcaataagaa
     6961 cgcatagcag atcaatgaaa tccaaaagaa aatttttcca ataagtggga agaagttccc
     7021 ggcagttaca gtgatgaccc caaatccaac gtaaatgata aaccagcttg gataaattgc
     7081 ttcaattgaa agatcagctt tgataacatg gtgataattg aaataaaaga caaggatcac
     7141 ttggaagata acagcgcaca gccaaatgaa tttgacaatt ggtgcaatac tttcatatga
     7201 aacaaaataa gtacaaataa ccatgagtga catcgtaaaa gttggtgaaa cggaggcgat
     7261 gattggatca tgcaacgttt gtttcgtgtg ttcgaataag ataaacaatt taacaaaaat
     7321 gaatatcata agaatcatag agataccgcc agttagattg cctaaagcaa catggtgata
     7381 gtctttaaac agatttccaa gggaagctaa cccaaggatc aacccgcaga tcgggatcgg
     7441 aataattttc aaaaagttct tcatatataa tattctcctt tttatttaat agattttagt
     7501 accaagatga acgaatacat tgtaaaatga ataggaatgt atgattacga gcaagcagac
     7561 agctgattgt cttcatctaa gttactagtt ggatcagcaa ctgttttgtt gtacatttct
     7621 attatagatg gttttttgta tatttaaatc aaatagtttt tatagtaatt atcataaaaa
     7681 gttatggaag tggtgccatg tttaagttat taacgacttt tcgagcagta tatgaaacaa
     7741 gaaatttttc cagagcagca gaagtactgt ttttatctca accagcagtc tcgaatcaaa
     7801 tcaaacagtt ggagcgggaa ttgaatatcg atctttttca acgtaacgga agaaaagaga
     7861 tgcttccaac taagcaagcc gacttattat atgaacggac actggttttg ttagaagaat
     7921 gggaagaaac tcagcaaaaa ttgctcaatg aacaaaatga agaagaagta tgtcggatcg
     7981 ttgcttccca cacctttgct gtctatcttt tgcctcagtt gatgaaacat ctgatcaaaa
     8041 ggtttcctaa agtgcatttt gagatcgaaa tcacgaattc tcatgaatca ttagaacaag
     8101 tagcgaaaca tgaatccgat tttggtttta tcgaaaagcc attagagact tccggcgtac
     8161 aaagatactc tttgatggaa gaccagctag tcatcgctgg ggacccaaaa agcaaattgt
     8221 ggcttgtaag agaagcaaac tctggtgttt ttcattacac gcagcgttat tttgaagaga
     8281 tgaatatcca agataagaag atgatcgtga aaagtaacga tgtaattgtt gcgttgctga
     8341 aggaaggaat cggtcgctcg attttgtcta agcgctctgt tccagaaaat gtgccaatga
     8401 ctccgttgaa tttccatcga tatttttatt tcatccaacg aaccaatctg gtttctgatc
     8461 agctgctaga gattgccgaa gagatccttc gatacaatga aagcacagaa acttctcaca
     8521 gcgaggagaa tctcagctgg tagataattg catgtatcaa aaaaagttct atgatacact
     8581 aaagataagc agtaggcttt tgtactgcat ttgctcaagc aactgcacaa acggaaagtt
     8641 aatttttccc aaatgtctat ggaaagactt tccattatct aactggaccg ctataataat
     8701 cagcagtatg gtttttaaga aaagggatga aacatggaaa aaaaattaaa agcttcgaac
     8761 gataatcgta ttgattatgg cgtgatttta ccagtctttc tgttatgttt gattggtctg
     8821 ctctcattat atgtagcact tagccatgat ccgaacaatc cagcagtctt gaaaggcgta
     8881 gggatgcaag ttgtctggta tttagttgga gcggcagcaa tcgttgtcat catgcatttg
     8941 aattccaaat ggatctggaa actgactcct tacctttatg gtttggggtt agtggtaatg
     9001 ggactcttgc tcaaattcta tgatcagaat ttagctgctt caacagggtc taaaaactgg
     9061 tttcgattcg gtgcgtttac tttccagcca gcagagttga tgaagattgc gtacatcttg
     9121 atgatggcac tgatcgtaac taaacataat acgcaagtaa aagaacggac gatgaaatcg
     9181 gacttttggc tgattgggaa gctcttgatc gtgacaatac cggttttagc attgatcatg
     9241 gcccaagatg actttggaac gatgcttgtc tttcttgcga tctttggcgg gattttcctg
     9301 atgtctggaa tctcttggcg catcatcgca cctgtagtaa tcttggcagt agtcgttgga
     9361 gcaggaacga tcttcttggt tacgactgaa ggcggaagag acttgcttta taaagttgga
     9421 ttcaaatctt atcagtttgc acgtattgat tcatggctgg accctttcca tgatacgtca
     9481 ggaatgagtt atcagccggc ccaaggactt ttggcaattg gtaccggtgg cttgtttggt
     9541 aaaggattca acgtaagcaa tatctatgtg cctgttcgtg agtccgatat gatttttacc
     9601 gttatcggtg aaaacttcgg ctttatcggt ggtgcattcg tcattttctt gtattttatt
     9661 ttaatctatc gtatgattcg ggtgtgtttc gatacgaata atgaatttta cgcctatatc
     9721 gcaagcggtt tgatcatgat gctgctgttc catgtctttg agaatattgg agcaaatatc
     9781 ggattacttc ctttaacagg gatcccattg cctttcatta gtcagggagg atcctcgatt
     9841 ttagggaaca tgatcggtat cgggttgata ttatcgatga gataccaaaa tgaagcacct
     9901 gcaagatctt caaggaggta agaaaattgt acacctttta ctggtacccg aaatgttcaa
     9961 catgcaaaaa agccaaagcg tggttagacc agcatggcgt tagttatcaa accgttgata
    10021 tgatcaaaga agtaccgaag gcctcaacga ttgagacttg gctagaaacg agtcaagtac
    10081 caatccgtcg cctgttcaac acaagcggga tgaaatatcg ggaacttgga ctgaaagatc
    10141 aagtaggcga gtttacggct tcaaaagcga gccaagtctt atcgacagat ggtatgctga
    10201 tcaagcgacc actgatctta cgagaggacc agcttgttgc aattggattc aatgaaaaaa
    10261 tttatgaagg agaactgata taaaatggca ggcaagcatt tgaagaaaaa ggacaacctt
    10321 tggatcttat ttaatggaaa agaatactgt gttggtttga caaatgaagc gcaagaagaa
    10381 cttggagaca tcacgtttgc caatctgcca aaagcaggac aatcgctaaa aaaagatgaa
    10441 cctctgatcg aagttgaagc ggaaaaagcg gtcaatgaat ttgccagccc gctaacgggt
    10501 acagtttctt ctgttaatga taaaatcggt gaagatatca atatcttgaa tgattcagat
    10561 gaaatgaatg cgtggatctt aagtttcaaa gatgttgatc caaaagaatt tgacgaatta
    10621 taaaatcaga aaatcacttg aaaaaagttg acactgattc ttccagttgc tataatcata
    10681 agcaatccta tatgaaacga gaaagctttg aaaagaagag tacatgtcac ggacgtttga
    10741 agagagccct gtttgctgaa aatgggtaac gaaaatgaga tggaagatgg tcttagagca
    10801 gagtaagcga gcgtaagcaa acttactacg ggagtgcccg ttacagcgcg acagtatacg
    10861 gaaagaagct aagtccaagt actggaagag gctgttattg cgaagtaacg gtaaatcaag
    10921 gtggtaacac ggaagttcaa gctttcgtcc ttttgcttaa ggcaagaggg cgaaggcttt
    10981 tttgtttgaa aaatcataag agaaagatgt gaatggcatg tcattaatcc aactaacgaa
    11041 tgtcaaaaag acattcaatg ggaaaaacgg aaacatccaa gccgtcaatg acgtttcact
    11101 aaacgttgaa aaaggggata tttatggaat cgtcggctat tctggcgcag gaaaaagtac
    11161 attagtccgt ctcctgaatg gacttgagct tcccacgagc ggcgaagtga tcgtaaacga
    11221 tcaagatatt acaaaattaa aaaacaaaga attacgtaca ttccgaaaga aaatcggtat
    11281 gatcttccag catttcaacc tgttatggtc aagaactgta cttgaaaata tccagctgcc
    11341 gctggaatta gctggcgtac caaagagcaa acgtaaagaa cgggcagaag aactgttgcg
    11401 tttagtcggg ttagaaggac gcggacaagc atatccttca caactttccg gcggacagaa
    11461 acaacgcgtc ggtatcgcac gagcattggc aaatgatcct gagatcctgc tttgtgatga
    11521 ggctactagc gcattggacc cgcaaacaac agaagaagta ttagacttat tgttagcgat
    11581 caataagaaa ttgaatttga ccatcgtctt gatcacacat gaaatgaatg tgatccgcaa
    11641 aatctgcaac aaagtcgctg tgatggagtt ggggaaagtc gtagaagaag gagacgtatt
    11701 gactgtcttc agacaaccga aaaaagaagt aaccaaacgc tttgtacaac aagacatcga
    11761 accagaagct gattcgacag aactattagc tgaactcatc aaagaaaatc caagcggccg
    11821 tttggcaact ttgaccttta gcgaaagaaa tgccaacgag ccagtcattt cccaagcgat
    11881 tcgacgctat aacatcgatg tcaacgttgt gtatggaaag atcaaacaag tgaaagaagg
    11941 ttcctttggt tcattgactg tcttgatgac tggtgaaacc aaagaattag atcaagcaga
    12001 agtgttcatc aaagaacaag gagtaggaat tgaggtgatc catcgtggat aaaagctttt
    12061 cagaaacata tctagatttt aaacaaatca acccggaaac gatgaatcag gctatccttg
    12121 atacactata tatgacatta atctcaatgg tcttagtgac gatcatcgga ttagttttgg
    12181 gattgatcct ttactctatt ggacgcaaaa acaaaccgta tgctcgaatc atttatggaa
    12241 tcgtatcgat catcagtaat attttccggt caacgccatt tatgatcctg atggttttga
    12301 tcattccatt tactaaagca atcgtaggca ctatgctagg tgcgcaagca gccattccag
    12361 ctcttgtttt atccgcagca ccattttatg cccgtctagt agagattgct tttcgtgaag
    12421 tcagccctgg tgtactggaa gcagcagatg caatgggagc aagttactgg gaaatcatct
    12481 ggaaggtctt gatacctgaa agtattcccg cattgatttc tggattaaca gttacaacga
    12541 tctcgatgat cggatttact gcgatggccg gcgcgatcgg cgcaggtgga cttggaggtc
    12601 ttgcttggca ggaaggctac cagcgtggta atctaacagt gacattcgtt gcgactttaa
    12661 ttattttact aatcgtattt atcgttcaag gaatcggtga tttcttaact aagaaaacag
    12721 acaaaagata ataggatata gggggatgta ggatatgaaa aagaaaattt ttggatttgc
    12781 agcagcgctt ttgctgactg taggattagc agcatgcggt aacaacagcg acagcaaaga
    12841 ttcagcaaat aaagcagacg acacgacatt gaaagtcggc gcttcaccga caccgcacgc
    12901 agagattttg gaacatgtga aaccattatt gaaagatgaa ggaatcgatc ttgaaatcgt
    12961 caaattcgat gactatgtat taccgaacca atctttggaa gaaggcgaca ttgatgccaa
    13021 ttacttccag catattcctt atttgaacaa agaaatcaaa gaaaaaggat atgactttgt
    13081 caatgccggt gctgtccaca tcgaaccaat gggcttgtac tctaaaaaag tcaaagatgt
    13141 ttccgaatta aaagatggtg caacaatcat tacttcaaat tctgaatcag actggggacg
    13201 catcatcaca attctacaag atgctggctt agtcaaagtc aaagatggag tagatctgga
    13261 aacagcaaca ttcgatgata ttgaagaaaa ccctaagaac ttgaagttca accatacgat
    13321 cgacccatca cttttagctt ctacgtacca aaatgatgaa ggtgacctag ttgcaatcaa
    13381 cgcaaacttc gcttacggcg cagggttgaa tccattagaa gatgccgtat tgcttgaaaa
    13441 ggacaactct ccttacgtga atattattgc gactcgtaaa ggtgatgaag acagtgacaa
    13501 aatcaaaaaa ctgattgaag tgcttcaccg caaagaagtc caagaatgga tcacagacaa
    13561 atggggcggc tctgttaaac cagtaaaggc tgatgcaaaa taaggtatgg aacaaaaaag
    13621 atgagaaata agtctgtaat ggcttatttc tcatcttttt tatacctatt ctaatttaga
    13681 aatcaaaatt gtttaataat aaataaaaga tatttgattt tagtaaggaa ggtcattgcg
    13741 cttcattctc aattagtcta aaatgagtga gaacgaaaga gaatttgggg gtataatgca
    13801 tgtctgtctt agaaataaaa aatctacatg tgtcgatcga ggaaaaagaa atccttaaag
    13861 gcgtaaacct aacaatgaaa accggagaaa tccatgccat catgggacct aacggcacag
    13921 gaaaatctac cttatctgcc gctatcatgg gcaatccaaa ctatgaagta acagaaggcg
    13981 aaattttgtt tgacgggaaa aatgttttag acttggaagt agatgaacgt gcgcgacttg
    14041 gcttattttt agctatgcaa tatccaagtg agataccagg tatcacgaat gcggaattca
    14101 tgcgtgcagc gatcaatgcg aaacgtgatg aaaacgacaa aatttctgtt atggcatttt
    14161 tgaaaaagtt agatgaaaaa atggctttac taaatatgcc agaagaaatg gctgaacgtt
    14221 atctgaacga aggtttttct ggcggagaaa agaaacggaa tgagatcctg caattgttga
    14281 tgttagaacc tacatttgca attttagacg aaatcgattc aggtctggat atcgatgctt
    14341 tgaaagtcgt tgctaaagga gtaaatgaaa tgcgcggaga caatttcggt gcattgatca
    14401 tcacccacta tcaacgatta ttgaactata tcacacctga tgtcgttcac atcatgatgg
    14461 aaggccgagt agtcatgaca gggaatgctg acctagctaa acgtctggaa gcagaaggct
    14521 acgctggtat cagtaaagaa ttaggaatcg actataaaga agaagaagcg taagggggag
    14581 caagaatgaa agaaaaaaac tggctcgatt atcttgacgc ggtaaatgat ttttctttaa
    14641 gtaaaggcga acctgattgg atgagaacct tccgtcaaga cgcactagcg aaagcagatg
    14701 aactgccatt gccgcatatc gatcgggtga aattccatcg ctggtcgtta tttgatgtga
    14761 aagaaacaca aaccatcagt gaaacaggca cgattccggc atttgatgca atgaaagaca
    14821 atcctgtgct tgtccaacaa ggttcatgga cgatttttga acaactgcct gtggaattag
    14881 cagaaaaagg agtcattttt accgacttgt tcactgcaat gatcgaatat ccagaactag
    14941 tccaagaata ttatatgaaa aaagcagtaa acatgaacga agaccaattg actgcactcc
    15001 atgtagcttt tatgaacagc ggtattttcc tgtatgtgcc taaaaatgtc gtgatcgatg
    15061 aaccactcga gtccttattc attcaagatg gcgcatccga tgagcacttc ttcaagcatg
    15121 tgttgatcgt ggcagatgaa catagtgaat tttcttatct ggaacgtttc cagacaacca
    15181 aagaacaagt agcaaaatca tcaggtaata tcatcgtgga agtcatcgct aaagctggtt
    15241 caaaaatcaa atattcagcc gtagatcaat taggagaaaa tattacttct tatatgaatc
    15301 gccgtgggca cattttacga gatgcttctg tcgactgggc aattggtgtc atgaatgacg
    15361 gtcatgtgat cgctgacttt gactcagatt tagcaggaga aggtgcacac gcagaagtca
    15421 aaatcgtggc aatcagctcg ggcagacaaa tccaaggaat cgatacacga gtaaccaata
    15481 aagcccctca tacgatcggg catattttgc aacacggagt gatccgagaa aaaggaacat
    15541 tgacctttaa tggtatcggc cacatcttga aaggggcaaa aggtgcagat gcccaacaag
    15601 aaagtcgtgt cttgatgctt tctgataaag cacgtggtga tgcgaatcct attcttttga
    15661 tcgatgaaaa cgaagtaact gctggacatg ctgccagtgt aggacgagtt gatccagaag
    15721 aaatgtacta tctgatgagt agaggattgc ataaagaaga agcagagcgc ttagtgattc
    15781 gaggattcct cggttctgtc ttaacagcaa tccctgtaga acaagttcgg aaagaattag
    15841 tcgaagtaat cgaagggaag ttgaatggat gatcgatgct aaaaagctac gcaatgattt
    15901 tcctatctta tttcaagacg tcaatgatga acctttagtt tatctggata atgcagcgac
    15961 gacccaaaag ccaaaacaag tactccaggc attagcgtac tattacgaac acgataacgc
    16021 caatgttcat cgcggtgtcc atacgttagc agaacgcgcg acccatgatt atgaacaagc
    16081 aagagaaaaa gtacgggcgt tcatccatgc aaaagaaaca gcagaagtgc tgtttactcg
    16141 tggtacgacg acaagcttga attgggtagc cagaaccttc ggcgaccgct atatcaaagc
    16201 aggagacgag atagtgctct cttacatgga acatcattct aatgttatcc cgtggcagca
    16261 gttggcaaaa cgaacaggtg ctgtcttgaa atatatcgaa ctaacagaag aaggcttcct
    16321 ggatatggag gatgcagcga aaaagattac ggacaaaacc aaaatcgtct ccattgctca
    16381 tgtatccaat gtcttaggtg tcttcaatcc tgttcaagaa ttagctgaac ttgtccatga
    16441 aaacggcggg gtcatcgtag tagatggtgc tcaatctgcc ccgcacatgg ccattgatgt
    16501 ccaagcacta gactgtgatt tctttgcttt cagcggacac aaaatgtgcg gaccaacagg
    16561 tattggtgtt ttatatggta aacgaaaatg gttagaagaa atggaacctg ttgaatttgg
    16621 tggcgaaatg atcgattttg tcgaattata cgacagtaca tggaaagaac taccttggaa
    16681 attcgaagca gggacaccaa atatcgccgg cgctatcgca ttaggccatg caatcgatta
    16741 tttgcaaaaa ctcggtatgg acaatatcca tcgatatgaa gaagaactag ccgcttatgt
    16801 gttgccaaaa ttacaagcaa ttgatggatt gactgtttat ggtccacaag acccgaattt
    16861 tcggacagga gtcgttgcat tcaatttgga tgggttacat ccccatgatg tcgcgacagc
    16921 tttagacatg gaaggggtag ccgtcagagc aggtcatcat tgtgcgcaac cgctattgaa
    16981 gtaccttgaa gtatcagcga ctgctcgcgc gagcttttat ttctacaaca caacgcaaga
    17041 cgccgaccgt ttagttgaag caatcatggc aacaaaggag tttttccaac atgggactat
    17101 ctaaattaga caatttatac cgtcaagtga tcttggatca ttcttcccat ccgcatcatc
    17161 gtggaagctt gactgactcg acaagcaaga tcgaattgaa caaccccaca tgtggagatg
    17221 tgatcgaatt gcaagtcgat gtaaaagacg gaatcatcaa aaatatcgcc tttgacggca
    17281 gtggctgctc catcagtacg gcaagtgcct cgatgatgac agacgccgtc atcggaaaat
    17341 caatagaaga agcagaacat ttaactgtag actttctaca gcttgtccaa ggaaaagaag
    17401 tcaaagatcc cgaccaatta ggcgatgctg ctatgcttgg tggtgtagct aaatttcccg
    17461 ctcggatcaa atgtgcgaca cttgcctgga aagcactagg aaaagcaatg gaaaacgatg
    17521 gtaccgcagc tttccatcat taccacaagg aagaggagtg agaacatggg cgtaccagaa
    17581 ttagaagaat ataaattcgg gtttcacgat gatgtcgaac cggttttcag tacaggagaa
    17641 ggcttgacag aagaagtgat ccgtgagatg tcaagaatca aaggcgaacc agaatggatg
    17701 cttgaattcc gtctgaaatc tctagaaaca tttaacaaaa tgccaatgca aacatggggc
    17761 cccgaccttt cagacatcga tttcgatgcc atcaaatatt accaaaaacc aagtgacaaa
    17821 cccgctcgtg actgggaaga cgtaccagaa aaaatcaaag atacatttga aaagattggt
    17881 ataccagaag ctgaacgagc ttatctagcc ggtgcttccg cacaatatga atcagaagtc
    17941 gtgtaccata atatgaaaga tgaattcgaa aagttaggaa tcgtctttac tgacactgac
    18001 tctgctttga aagaataccc tgaattattc aaagagtact tttcaaaact tgtaccgcca
    18061 accgacaaca aattagcagc gctgaattca gccgtatggt caggtgggac ctttatttac
    18121 gtgccaaaag ccgtcaaagt cgatgttcca ttacaaacgt acttccggat caatgcggaa
    18181 aacacggggc aattcgaacg tacgctgatc attgttgacg aaggagcaag catccactat
    18241 gtcgaaggtt gtacggcacc tacttattca agtaacagcc tgcatgccgc catcgtagaa
    18301 atctttacaa gaaaagatgc gtattgccgt tacacaacga tccaaaactg gtcagacaat
    18361 gtgtacaact tagtaaccaa acgagcaaaa gcatacgaag gcgcaaccgt tgaatggatc
    18421 gacggaaacc taggtgcaaa aacaacgatg aaatacccaa gtgtttactt agatggaaaa
    18481 ggtgcacgag gcaccatgct ctccatcgcc tttgcaggag caaaccaaat ccaagatacg
    18541 ggtgcaaaaa tgatccacaa tgcaccaaat acatctagct ctatcgtctc taaatcaatt
    18601 tcaaaagacg gcggagaagt aaactatcga ggacaagtaa cctttgccaa aaacagtgcg
    18661 ggttcgatct ctcatatcga atgtgacacg atcatcatgg acgaatggtc gaaatccgat
    18721 acgatcccat tcaatgagat ccacaacagc caagtctctt tagaacacga agccaaagtc
    18781 tcaaaaatct cagaagaaca actctactat ctgatgagcc gaggcctgac cgaatctgaa
    18841 gcaactgaaa tgatcgtcat gggctttgtt gaaccattca ccaaagaact tccaatggaa
    18901 tatgcagtcg aactcaatcg attgatcagt tatgagatgg aaggcagtgt tgggtaaacc
    18961 tgttatagga gctagaaccc ttgatatata agggttctag cttttttgtt ttgcctgtat
    19021 actagaaatg accagatttt gaccagctct tttactgatg agccatcata tcagcaaagt
    19081 cttgacctgt tttcttaggt gcattatttg taatatgtgc atagatgtct aatgtcactt
    19141 tactattttc atgccctaat cgttgttgaa ctaccttgac agattctcct gcttctaata
    19201 ataaactagc atgtgtatgt ctaaacccat gaggtgtaat acgaggcaag ttatattttt
    19261 tcaaaatata tcttaaccaa tcattaacag tgtttggaat gtatagagtg tttttaactg
    19321 ttgtaaaaac atgttgctct ttagagctag tattgtatcc aagttttaaa tactcttctt
    19381 tttgctgtaa tttccagttg ttgaggatag aaagagtctc agtatctaaa gagataactc
    19441 gtctagaagc tcttgttttc ggagattgaa ttataacttt accaaattca tctacagcaa
    19501 gagttttatt gacatgtagt tctttattaa acgtgtctat gtctttccac tgtaaagcga
    19561 gtacttcact ctttctcata cctgtgtatg ccaataatct gaagaaggcg tattgtttca
    19621 tgttaccaaa atctttaaaa gcatccagta aattattaag ctcctctttt gagtaaaatt
    19681 tacgattttt ctcatactct ttttttcgtg gaagaagtgt tttttccata ggattattgt
    19741 caatatattg cattgcaacc ccgtagcgta aaatttgtgc agtctgtttt ctcatataat
    19801 cgtaactttc atatttactg tgccattcat ctaccacttt ttgacaatat gggacagaga
    19861 tattcgatag tttaagctta ccaaatgctg gaagtatttg attatttgcg tatcttactg
    19921 agattgcgat tgttgaagct ttaacactgg tacgctgatg agttatccat tcattataca
    19981 ggtcttcaaa tgtcatatca ggtgctttct ttttaacacc gtataatatt tgatgtctgt
    20041 actcgtcata atctttttga gcttcttgtt tgctactaaa acctcttctc gtagtattta
    20101 ttttttcacc agttcgtgga tcggtggcaa tgtgtccttt atacatccac gccacttgac
    20161 cgtttttctt tttgtacttt gatatttttg ccattaaatc atctcctctt agtttacaag
    20221 gagtacttac cactagatgg tatcacctcc cttcattttt ttcttagtgt attttttccc
    20281 aattgatgaa atcgtttgtt ctagcttgtt tatattatca gtaatttctc tcacagcttc
    20341 ttcgggataa gacatgtctt tcaattgaat aggtagccat tcatctacca ttctttctag
    20401 tagttcttct atactgctag tgaggaaaag aatgttattg tcaaattctt ctacaatagc
    20461 attgatgaat aattcttcta tttttaagat attgaaaaga ctaatttttt catttttaac
    20521 tttattacaa gtcttgttag caataaattc ttgcatctct ggttctaatt tggaatatct
    20581 atccagccat gtatctaaat tagagtgtac tggtgggaac tgttgacgaa gatgctggaa
    20641 caggttttta tctggaaatg ttataccatc tagtacaatg ctatctctat aaagaataat
    20701 tcccaaaata tattcttcta cattgccaca aactaactcc tctatactca tatttcccaa
    20761 atcagcaatt cgggataatc gctctggtat tggtaaatag gttcccttct cccattcgta
    20821 gatagttttt ttgtcaatta attgaccgtt gttaatcata agaccaaatg ctggttgtgt
    20881 taatttttta ctctttctta tagcttttat ttttgttccg agtatctttt ttcgttctaa
    20941 ataatttatt ggtggacgtt ttatcatatt gaccacacct cctaaaatta gtatacctca
    21001 aaaaaggggt gttgtaaata aattttacaa agctgttgac accccttttt tatcgaggta
    21061 ttatgtgtat gtcgatgaac tggtaatcat tttttgtttg attaatcggt taataacaaa
    21121 atacttttat tttgagcaaa aaatacgaag ataattctta gaaaatttgt tcaagcaatc
    21181 acgaaaggag ggggaaatat gtctctacaa atcattgata gcattgtttc tatacaagtt
    21241 gaacaacaag tgaaagagat aattacagtg ctaaagccta tgctagaaga cattatttct
    21301 ggagtcactc tagagtatat ggactatcag caggcaagtg aatatctagg tgtatcagta
    21361 ggaacgatta gaaaatatgt ttctcaatac gggttacctg ttataaaaat tgatacggtc
    21421 attagattta aaaaatctga tattgatagt ttcatggaac ggtataaagg ttgagtaaac
    21481 aa
//
LOCUS       DAHUVV010000043        21250 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.043, whole genome shotgun sequence.
ACCESSION   DAHUVV010000043 DAHUVV010000000
VERSION     DAHUVV010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.043"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..563)
                     /locus_tag="NK767_001569"
     CDS             complement(<1..563)
                     /locus_tag="NK767_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCH6694568.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="NK767_001570"
     CDS             complement(750..1400)
                     /locus_tag="NK767_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694569.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="NK767_001571"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="NK767_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCH6694570.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="NK767_001572"
     CDS             complement(2912..3910)
                     /locus_tag="NK767_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCH6694571.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="NK767_001573"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="NK767_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCH6694572.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="NK767_001574"
     CDS             complement(4926..5657)
                     /locus_tag="NK767_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HCH6694573.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="NK767_001575"
     CDS             complement(5661..6926)
                     /locus_tag="NK767_001575"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HCH6694574.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="NK767_001576"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="NK767_001576"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCH6694575.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="NK767_001577"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="NK767_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCH6694576.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="NK767_001578"
     CDS             12268..13770
                     /locus_tag="NK767_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HCH6694577.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="NK767_001579"
     CDS             13772..14509
                     /locus_tag="NK767_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCH6694578.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="NK767_001580"
     CDS             complement(14680..15873)
                     /locus_tag="NK767_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6694579.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="NK767_001581"
     CDS             complement(15952..16158)
                     /locus_tag="NK767_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCH6694580.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="NK767_001582"
     CDS             complement(16151..17293)
                     /locus_tag="NK767_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCH6694581.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="NK767_001583"
     CDS             17805..18440
                     /locus_tag="NK767_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6694582.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="NK767_001584"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="NK767_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18916..19032
                     /locus_tag="NK767_001585"
     CDS             18916..19032
                     /locus_tag="NK767_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCH6694583.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            19048..20967
                     /gene="tet(M)"
                     /locus_tag="NK767_001586"
     CDS             19048..20967
                     /gene="tet(M)"
                     /locus_tag="NK767_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCH6694584.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            21086..>21250
                     /locus_tag="NK767_001587"
     CDS             21086..>21250
                     /locus_tag="NK767_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCH6694585.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
BASE COUNT         6286 a         4329 c         3776 g         6859 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
    21001 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggagat
    21061 agttattata aactacaagt ggatattgtg tcctgtatgt ggaaataaaa cacgattaaa
    21121 gataagggaa gatactgaat taaaaaaatt ccccctctat tgtccgaaat gcagacaaga
    21181 aaatttaatt gaaataaagc agttcaaagt aactgtgatt acagagccag acgcaaagac
    21241 gcagagccga
//
LOCUS       DAHUVV010000044        20961 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.044, whole genome shotgun sequence.
ACCESSION   DAHUVV010000044 DAHUVV010000000
VERSION     DAHUVV010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.044"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(262..2259)
                     /gene="tkt"
                     /locus_tag="NK767_001588"
     CDS             complement(262..2259)
                     /gene="tkt"
                     /locus_tag="NK767_001588"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCH6694586.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2361..2612)
                     /locus_tag="NK767_001589"
     CDS             complement(2361..2612)
                     /locus_tag="NK767_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HCH6694587.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2759..3382
                     /gene="lexA"
                     /locus_tag="NK767_001590"
     CDS             2759..3382
                     /gene="lexA"
                     /locus_tag="NK767_001590"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCH6694588.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3562..3780
                     /locus_tag="NK767_001591"
     CDS             3562..3780
                     /locus_tag="NK767_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694589.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4069..4581)
                     /locus_tag="NK767_001592"
     CDS             complement(4069..4581)
                     /locus_tag="NK767_001592"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCH6694590.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4595..6892)
                     /gene="recJ"
                     /locus_tag="NK767_001593"
     CDS             complement(4595..6892)
                     /gene="recJ"
                     /locus_tag="NK767_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCH6694591.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7034..7477)
                     /locus_tag="NK767_001594"
     CDS             complement(7034..7477)
                     /locus_tag="NK767_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HCH6694592.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7478..8266)
                     /locus_tag="NK767_001595"
     CDS             complement(7478..8266)
                     /locus_tag="NK767_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCH6694593.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8263..9204)
                     /locus_tag="NK767_001596"
     CDS             complement(8263..9204)
                     /locus_tag="NK767_001596"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCH6694594.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9381..9968)
                     /locus_tag="NK767_001597"
     CDS             complement(9381..9968)
                     /locus_tag="NK767_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HCH6694595.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10189..11496)
                     /gene="obgE"
                     /locus_tag="NK767_001598"
     CDS             complement(10189..11496)
                     /gene="obgE"
                     /locus_tag="NK767_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCH6694596.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11790..12800
                     /gene="gap"
                     /locus_tag="NK767_001599"
     CDS             11790..12800
                     /gene="gap"
                     /locus_tag="NK767_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCH6694597.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12885..13820)
                     /locus_tag="NK767_001600"
     CDS             complement(12885..13820)
                     /locus_tag="NK767_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCH6694598.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            14006..14965
                     /locus_tag="NK767_001601"
     CDS             14006..14965
                     /locus_tag="NK767_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HCH6694599.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(15051..16145)
                     /locus_tag="NK767_001602"
     CDS             complement(15051..16145)
                     /locus_tag="NK767_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCH6694600.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16334..17095
                     /locus_tag="NK767_001603"
     CDS             16334..17095
                     /locus_tag="NK767_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCH6694601.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17210..17650)
                     /gene="fabZ"
                     /locus_tag="NK767_001604"
     CDS             complement(17210..17650)
                     /gene="fabZ"
                     /locus_tag="NK767_001604"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCH6694602.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            17870..18625
                     /gene="fabI"
                     /locus_tag="NK767_001605"
     CDS             17870..18625
                     /gene="fabI"
                     /locus_tag="NK767_001605"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCH6694603.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(18735..19748)
                     /locus_tag="NK767_001606"
     CDS             complement(18735..19748)
                     /locus_tag="NK767_001606"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCH6694604.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            19913..20833
                     /locus_tag="NK767_001607"
     CDS             19913..20833
                     /locus_tag="NK767_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCH6694605.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         6061 a         4500 c         3710 g         6690 t
ORIGIN      
        1 catatcccta gaaaatcact aaattaccaa acaccattag aagttttttt gagttacgta
       61 aatggaaagt tttgtctcgc ttaatttgac aaataatatt ctcaagaaac acaaactaat
      121 ccgaagaatt ctttgttttg tcatttaaat gtaacatagc cgaaaacgaa aagtaatatt
      181 gatttattct tctaacacat aaagaacagc tgtctccaac ttgcagacag ctgttcttat
      241 cagtcaaaca attgcttgga tctatgatag ttgattgtac gtattcacga cattttctac
      301 tgtaaatcca aattcttcta aaattttagt gccaggggca gatgcaccga aatgatcgat
      361 tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat ccaaatgatg cagcagcttc
      421 gatagccaca cgtttcttca ctgatttagg aagtacactt tctttatatt cagaagattg
      481 tttttcaaag aggtcaaagc ttggcataga aacaacagaa acatctttgc ctttttcagc
      541 caattctttt tgagctttca cagccaaatc aacttcagaa cctgttgcaa tcaaaatacc
      601 ttctggtgtt tctccttgtg atggagaaag aacatacgcg ccttttttca ccatttcatc
      661 tgctacttct tttgtagaag gcaatactgg aaggttttga cgactcaata caaggatagt
      721 aggcgcatct gttgtctcca tcgctacttt ccaagcagca cgtgtttcat ttccgtctgc
      781 cggacgaatc acttgaacac caggcatgca gcgaacagat gctagttgtt caatcggttc
      841 gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt gttaagacgt aaacaacagg
      901 agtatgttgg attgctgcta aacgaactgc tggtcttaga taatcgacaa agacaaagaa
      961 tgttccaccg tagatacgag ttccgccatg aagttggata ccattcatag ccgaagccat
     1021 tgcaaattcg cggacaccga accagatgtt gcgtccttca taatgttcag gagtgaaatc
     1081 tttatctgca gccaccattg tattattgga acctgacaag tccgctgaac caccccagaa
     1141 gcttggtata gctttcgata attcttggat gacttcttta cttgaaacac ggcttgcttg
     1201 actgcttcca acttcataag ttggcaattc tgcatcccag ttttctggta gtttgccatc
     1261 aaaagcatct tcaaattgtt gtgctaattc aggataagct tttttataat tggcaaacat
     1321 ttcattccat gcatcttcag ctttttgtcc ttcttctatc atcgtttgat ggaagcgggc
     1381 agctacttct tctggaacag tgaaatctgg atactcccaa ccataaactt ctttagccat
     1441 cttgatgcca tcttcaccaa gaggagcacc atgaacagca gatgttcctt ctttaggagc
     1501 accatagccg atgactgttt ttacttcgat caatgttggt ttgtctgttt cagcttttgc
     1561 ttcttcaatc gctttagaga ttgcttctag gtcatttccg tctttcacta agatatgttg
     1621 ccagccatat gcttcatagc gagcaccaac gttttctgtg aaagcttttg aagttggacc
     1681 gtctagtgaa atatcgtttg aatcgtataa aacgatcaat ttccctagtt tcatatgacc
     1741 tgccatcgaa cttgcttctt gagatacccc ttccatcaag tctccgtcac cgcatattgc
     1801 atacgtatag tgatccatca cattaaagtt ttctttgtta tacgttgctg caaggtgcgc
     1861 ttctgccatt gccataccga cagccattgc aatcccttgt cctaatggcc ctgtagtggc
     1921 ttctacacca tctgtatgat gtacttcagg atgtcctggt gttttagaat cccattgacg
     1981 gaattgtttc aaatcatcga ttgtcacttg atagccagct aaatgaagca agctatacaa
     2041 cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg cggtcagccc agttttttga
     2101 tgtcgttgga tttactttca ggtgttttgt ccataacgca tatgccatag gtgctgcgcc
     2161 catcggcaat ccaggatgtc ctgagtttgc tttttggact gcttcaatac ttaatgtacg
     2221 aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa ataacccctc ctattaattt
     2281 aggttttttt tacctttatt tcttacactt tcattctata agatgtcaca cgaaaaagca
     2341 atctttttca gtatttttta ctaaaagttg tcatttcggt tgtgtaagcc ttttttcttt
     2401 tggatttctt ttaatttttc cggggtcacg tcattgccct caggatcgac gactttcatt
     2461 ccttcgatat gatgacgcat accggaacga aaagcttcca aatactcttt gcgcaagttg
     2521 atttgttctt gttcttcggc ttttgagaga ccattttcct tctttttacg tgcaagttca
     2581 ttgatccgct ctattttttc aggtgacagc atgctcagcc ctccttttac tctccttcat
     2641 attatataaa aactgaaaaa aaaacaacta gataaactaa ataaaatacg aacattcgtt
     2701 tgatttagcg ctttttatat ggtactattt atttatcaag tgaaagaagg tgtaccttgt
     2761 ggcccgacaa actgaatcaa gacaaatcga agtcttacga tatatccatg aacaagtaac
     2821 ccaaaaagga tacccgccga ctgtccgtga aatcggggaa gctgttcagc tttcttctac
     2881 ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa gggtttatcc aaagagatcc
     2941 gacgaaacct cgtgcaatcg agttgacaaa acaaggttta gaaaagattg ggattcgtcc
     3001 aacaactatt ccagtacttg gcgtcgtaac agctggagaa cccattttag cagtagagga
     3061 tgcttcagac ttctttccaa tccctcctaa tctttcttcc gaagaaggca gtctctttat
     3121 gctgacaatt cgtggagata gtatgatcaa tgctgggata ttggatggtg acaacgtgat
     3181 cgttcggaag caagaaactg ctcaaaatgg cgatattgtc attgcaatga ctgccgagga
     3241 cgaagcgact tgcaaacgat tctataaaga aaaaaacttt ttccgtctgc agcctgaaaa
     3301 cgacttgctc gaaccaatca tcttagatca agtatctata ttaggacgag tagtaggact
     3361 ttatcgtagt catatctatt aattgctact aattaaaaga aaacttttta aaaagagttt
     3421 gagacgttgg tctcaaactc ttttttcgcc cgggttcttc ttctttcaaa tttttatcac
     3481 ttccctcttt acaaaccgaa caaatattcg tatactaaat atacgaacaa ttgttcgcat
     3541 aaattaaaga ggtgagaatt catgaaatca atgagacgtc ttggcttagt attaactgtt
     3601 ttgtttttag gtattattat tggtaatttc ggcatacgtc cagctttact tgtgtttgtt
     3661 ccgttattta ctttgtggtt catgttgtgg gatgaaaaga agtaccgtca atctgagcgg
     3721 cgcagaattc atgaagatca tttctatcat gagccctact acagatccag gcaatcataa
     3781 taaggattga ctgctaatta ttcaaaagac aagaagttaa acttttacgg gggtgtgaca
     3841 aaaatcggtc acatcctttt tttcgaataa acggtgttct aaagccagtt tttcggtatt
     3901 aagtcaaatt gcggaaaaag tgatttttta aacttctact ggtctttgct tgtttccatt
     3961 tataaaaaaa tagcgaccct caaaagtcag atagagaatc tgacttttga gggtcgcttt
     4021 aaaatcgctt ctttaagaat aacatcaaga atagcttctt ttttctgctt aatattccat
     4081 taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca tggagatcca tcaattcgat
     4141 caaaaatgcg cagcctacga ctacaccgcc taattgttcg atcagttcga tcgtcgcttt
     4201 gattgttcct ccagtagcta gcaaatcgtc acaaatcagc acacgttgac ctggtttaat
     4261 ggcatcctta tgcaatgtca atgtatctgt tccatattct aatccataag tcacttcgat
     4321 ggtttctcga ggtaacttcc cttttttgcg gactggggca aaaccgactc ctaactcata
     4381 agcaacagga catcctacga tgaatccacg ggcttctggc ccaactacca tatcaattcg
     4441 tttttctttt gcataattta cgatttgttt agtagcttcc cgataagcgt caccatctgc
     4501 catcagtggg gaaatatctc gaaacacaat tccttttgac ggatagtcgg gaatactggc
     4561 aatataatct tttaaatcca ctctttgttt cctcctaggt tgttaaccat ttctttatgg
     4621 taggaatatc gctcaaaagc aaaaattcct caactttgat tttattgagc cgtcgctggt
     4681 ataatctgct ctcagtcaaa ggatgacttt ctgggctagc gactttttgt aaaacaccgt
     4741 tctctattgt aacaaatttc aattcaaaaa acacctgtat cataaaaatt aataattttt
     4801 cagggatacg caaatattga gctattgctt ttattttatg acgtatatca attcgttctt
     4861 gagaatggat caatttaaac aattttgcgt actgttctct tgtgcctacc ccattcaaat
     4921 aggcctcatc aggtgaaatc cccatcagat aaacacgatt gaatgaataa gaccgaaaaa
     4981 cttctttcag atccgctaaa tcatctggac aatccataac aactagagag tttggttttc
     5041 ttttttcaat tattgccttt acagaatcta gtccttcgaa aacaatcgca gcttcctgga
     5101 taatctcagg cacgaatttc aacgtcgaag aatcaaatgc taaataaagg ctaccttccg
     5161 acgttcggct ctgttcacgg aaacgttttg cccgccaatc aaatacttgc agaccaccca
     5221 cagaaaaatc agataccatc agctgcggtt tcttacgccc gttccattca ttgatcgaaa
     5281 gctttcctac cacatctacc tgattattca gcagttcttc ttcttgagta ccaaaaccaa
     5341 aagcgaccgc atcaagctga ctcgatgcat cggaaaggac aaatttcaga tgctgttggt
     5401 ttgctccgat tcttttgata ttttctgctt ggacttgtcg aaataaaaac tgaggcaaag
     5461 gattatccgt accaaatggc gccaatagtt tcagctcatc aattcgctct actgttactt
     5521 catttggcag caatacttca tcaattctca actcaggccc tctcatcaag tcaatttgat
     5581 gatcgactat atattggttc atcttctcct gtagtatagt gacattttca cttggcatcg
     5641 ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa taaatctcgc atctggtcaa
     5701 gcatttcaaa aagattcaag gcttcaatac tacgtccaga tcctttagct gatccatctt
     5761 cttttaaagt aaggacaagc gttggtttac ccgtttcatt cattattttc ccagcaacaa
     5821 tgcccaaaac gccttcatgc catcctcggt ttaccaatag atggatatga ttctccttat
     5881 tgaccatcgc taatgcttct tgtgttatct gctctacgat tgcttttctt tcttcattga
     5941 tctcattcaa tttttttgct tgaacggatg cttcctcttc atcaaacgtc gcaagtaacg
     6001 agacggcagg acgtggatcg cccatccgac cgattgcatt tagtctggga gcaatcgaaa
     6061 aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc gctttcttca aatagcgcct
     6121 gaagtcctat ccgttctgaa tgatgaatgg catcgattcc taatgaaaca aggattcgat
     6181 tctcatcagt caatgaaact aaatcggcga tcgttcctat cgctactaaa tccaaaaatt
     6241 ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa tgctactccg actcctgcca
     6301 aatctggaaa tggatagtgc cccgctggat gacgagggtg aacaatcgca taagcgtctg
     6361 gtaaattctc aggtagttcg tgatgatctg taatgatcac gtctacacca gcttgctgag
     6421 cgtatgctac cgcatcattt cctgctaccc cgttatcaac agtgatgatc aactggattc
     6481 cttcttcgat cttttcttgg tataccgatt ggtttggtcc atacccatgc tcaaatcgat
     6541 ttggcaggaa tacttctact tctgcaccaa gtaattccag tgtttctttc attaccgttg
     6601 cactggtgat cccatctgcg tcataatcac cgtaaacgag gattttttct ccactgatta
     6661 cagcttcttg gattcggctg attgctttat ccatatcgtg tatcagatac ggatcatgta
     6721 attcttgttc ctttggatgt aaaaatcggt tgatatcctt ttcctcatgg taacctcttt
     6781 gccacagtaa cttaccgaca aatggagata atttcagttc acggatcatt tggagaaagg
     6841 attcagaagg ctctgtctcg cttaacttcc aatcaaaatt tgcttgtctc actttctcac
     6901 tcctaactta gttattatag caaaggaaat cagcaaaaaa aagcagaaag aaagaataaa
     6961 aacagatcag atccgctaga cagatgttca gcaggacctg atcttttgta cgaccattta
     7021 tgaaatatcc ctattagtca aaataattga agctttgttt gggcgattga gttttttgtt
     7081 cttccggcgc tttcaattga ctgatttgtg cggttttcgc ttgtaactcc gcctgataag
     7141 cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg ctgttcttct tttgcttctt
     7201 caattttctt ttgaagttct gtttgatatt ctgacatttc ctgagtcagc tgtttgattt
     7261 gctttttttg ctgccatagt gttgccgaag cagtcaaaaa tacaatcagt gcaccaatca
     7321 gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa ggaagtgaag ccaaaactta
     7381 caggaacatt tttattgttc aatacagcaa aaagaacaat aatcaaaact aaaacaaatc
     7441 caagaatcac attgctttgc tttttcaatt ccttcaccta ctttttgtta aaaattccgc
     7501 tggctaaata atcaccaatc gttggaaaca gcgtataaaa ttttgatgca acttctaaaa
     7561 taaacggttg attgatttcc cgttttggat gtctcatatt gcggacgatc atttttgcca
     7621 acttggcagg ttctaaaacg atttgtccga ctttttccaa atagcttccg cttggatcag
     7681 ccttatcgaa gaaatttgtc tcgattggac ctggattcac tgtcgtaaca gctacgccaa
     7741 gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag gactgcaaat ttcgtggcag
     7801 aataaaccgt tgattttgca gttgccattt ttccagccat agaggcgata ttaatgatat
     7861 gaccttgtct tctttctgcc atatcgatcg caaatttttg tgtcaaaacc atcattccta
     7921 agatgttgac ttcaaacata tcgtatgctt ttccaaggtc gaatgtaagg aagtcttcga
     7981 aaatcccaaa acccgcattg tttaccagaa tatcgacgcg cccgacttct tccatcacct
     8041 tttcatataa ttcgtccacg ctttcaggat tgctgacgtc taccttgaaa gcataagcct
     8101 ctttgccgct caagcgtctg cactcgtctc tgacaccttc tacaaatgcc attcttcgtg
     8161 cgcaacttat aacgattgct ccttgctttg ctgcttcata acagatttgt gcgcccagac
     8221 cgcctgaact acctgttact aaaacaactt tatcttctaa attcatgcct cttcctcctc
     8281 gcgaaatgga atctcaatgc tatcaaaatc tttcataatc gtcgtgtttt caaacacttc
     8341 ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa tatctcgcac tgatatgagt
     8401 aagtagcagc cgttttgcct gcgcttcttt tgcaacttca gcagcttgat gagtcgtgga
     8461 atggaaatag ttgtgcgcca ttcttgcttc atctttatta aatgtgcttt catgtaccag
     8521 aatatcgcta ttttccgcaa gcacaacgga attatgtgtt tttctcgtat cgcctaaaat
     8581 agtcacgatt cgtccttttt tatctggacc tacgaaatct tttccattga tcgtccgccc
     8641 atcttctagt tggatcgttt caccttgctt cagtttgccg tacagtggtc cagctggaat
     8701 acctaattct tttagacgat ccacctgcaa ttctccttta tgatcatgtt caaccacacg
     8761 ataaccaaaa ctgtcgatcc catgatttag tttttttgta taaacagaaa attgctgatc
     8821 agtaaaaatc ggggctgttt catttagctc aatgaatttc agcggataac ttaaacgcgt
     8881 ttgcgaaata cctaaagaga cttttatgta ttcctctatc ccttttggcc cgtaaatctc
     8941 taatggcgta tccccacctt gaaatgaccg gctgctgatg agcccgggaa gtccaaaaat
     9001 atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt tttctcggac gaatagtcgt
     9061 tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac aaccaaactt cattgcgctc
     9121 gtctaaaagt ttcaatgcta tacttgtcac atttctatgt tttgcaggaa cacctgcacc
     9181 tgttcctaaa aattgtaatt ccataatcta cctgactttc tttaagttcc tctttaattt
     9241 tagggtaaaa agaagggata atcaaccgta agtcgagaat gggttcaaat taaaaataac
     9301 gttctaaagc gatagcgttt tctttgaaaa ttactataaa aacagcagcg aacccgtttg
     9361 agatcctctg ctgtttttta ttaatttgaa gatccagaac tttcattgtc gttggaagtt
     9421 tgcggatgtt tgtccttcca agtcttttgt gcagcttctt tcgcatgatt ttcagcttct
     9481 ttcgcatcat gcacatcttt ttcgacatct tcataatcca agcgagtaat ttctccgcct
     9541 aattccatca ttaccaattg attcagtggg cgattgtcct cttcatcagc aatcaagatc
     9601 agtcctgtag ctccttcacc gattttattt cctacaaagt cgaacacggt ctgggcatct
     9661 tttatttctt tggcatcttg tgtcgcaccg atcattcctc cggcaaacca tcctaataaa
     9721 atccctagcg gaccacctaa tacaccaacc aacattccga ttaatccacc cgtagaagat
     9781 ttgttattac cggtaaagtc gataaaatct tctattttga attgatgtgt cccattagga
     9841 tcatggctga caactgccat ctgttctcct ttaatcgaac gaacagcatg taactttttg
     9901 atttctgaaa atgcctgata tgcctggctt tctacttgaa aatttaaaat gattactcgt
     9961 ttgctcatta aaaacgcccc tttcatacct tcttgttatt tattttacct ttaattgaaa
    10021 aataagaaaa gaaaagcact ctttttttcc aataaaatag ggctgtgaca aaagtcgtat
    10081 cacagctcct ttttccgaat aaacggcgtt cttgaagctg atcacttctg tcacaacctc
    10141 ccttaaatct gaatctatat tttttggtat ttgtttttat ttgcttgcct attcgacaaa
    10201 ttcaaattca aactcaccga tacgtacaag gtcgccatct ttcgcaccgc gtgcccgaag
    10261 agcttcatcc acacccatac cacgtaattg acgagcaaaa cgcatcactg tttcatcatg
    10321 atcgaaattc gtcatttgga ataatttttc gatcttctca ccagataaga tccacgttgc
    10381 atctgaatca cgatcgattt ggaactcagg accttctgga ttgaatccat agtgtacagt
    10441 ttcttcttcc agttcttctt cgtacaatgg gaattctggc gttacttcta acaaatctgc
    10501 tgtagcattc aataacgcgt ccaatccttg tcgagtcact ccagaaattg gaaatactgg
    10561 gatgtcatca gcaaattcat cttctttttc cttgttcagc tgttctttga atttcactag
    10621 attttccggt gcatccggca tatccatttt gtttgccacg atgatttgcg gacgctctag
    10681 caagcgtaag ttataagtag ataattcttt gttgatggca agatagtctt cataaggatc
    10741 tcgtccttcc atcccactca tatcgatcac atgtaaaatt actcgtgttc tttcgatatg
    10801 acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct ccttcaatca agcctggcaa
    10861 gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc attcctagat taggtacgag
    10921 cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat gaaataacgg atagtaaagt
    10981 cgatttcccg acagatggaa aacctacaag tccaacgtct gccaatactt tcaattctag
    11041 ttcgattttt cgctcttgtc ctggttcacc gttttcagca atttccggtg caggattccg
    11101 aggagaggca aaacggatat tccctcgtcc accgcgtcca ccttttgcca caactagtgt
    11161 ttgaccattt tcaattaaat ctcctaaaag agcaccagtc tccgcatctc ggacagtggt
    11221 tccttgtggt actttaacat aggtatcttc tgaaccacga ccgtgcatcc ctttactcat
    11281 cccattttca cctggctgcg ctttgaaatg gcgattgaac cgaaaatcca tcaatgtacg
    11341 taacccttca tcgacgatca agatgacatc gccgccacga ccaccgtctc cgccagccgg
    11401 tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc attccgtctc cgcccttgcc
    11461 agctttcaca tcgatcgtta cttgatctaa aaacatggac ataattaagt cctcctatta
    11521 ttcatttatt tatttgactg cctgaatttc cacaataaac aaaagtctta cttattttat
    11581 cggtgaaatc tgattttgtc tatcatttca gcaaaaagat tgtttacctt cttctttttt
    11641 tatcatttgg taaggtttcc aacctttttt ctgctaccaa gtataatttt ttgacaaaat
    11701 attctttgcg tagcagaata acagtgtatt aattgtatta attgaggatg ccgtttcaca
    11761 gcacccctaa taaaggagga acttttgata tgacagtaaa agtaggtatt aacggatttg
    11821 ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt ctcagatgat attgaagtag
    11881 tagcaatcaa cgacttaaca agtccaacta tgttagcaca attattgcaa tttgactcta
    11941 ctcatggtac ttatcctggc acagttactg caacagaaga ctcaatcgta gtggatggcg
    12001 aagcaactcg tgtttatgca gaacccgacg caagcaaaat cccttgggca aaagaaaacg
    12061 gtgtagatat cgtactagaa tgtactggtt tttatacatc tgaagaaaaa gcaaaagcgc
    12121 atttagatgc tggtgtcaaa cgagtagtta tttctgcccc agctggtgct atgaaaacca
    12181 tcgtctataa cgtcaatgat gatacattag atgaaaacga caaaatcatc tcagcaggtt
    12241 catgtacaac aaactgttta gcacctatgg cttatttctt aaataatgaa tttgggatcg
    12301 aagttggtac aatgacaaca gttcatgcct acacttccac acaaatgctc ttagatggac
    12361 ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc tgacaatact atcccacatt
    12421 ctactggtgc tgcgaaagca atcggcttag ttatccctga gctacaagga aaattacaag
    12481 gacacgcaca acgtgttcca gtcgttgatg gttcattaac tgaattggtt tcagtattga
    12541 aaacaaaagt aactgctgat caagtaaacg aagcaatcaa aaaacacaca gttgacaatc
    12601 cttcattcgg gtatgatgac cgtcaaatcg tttctagcga tgtcattgga acaactcaag
    12661 gttcgatctt tgacccaacg caaacagaag taacaactgc tggtgatttc caacttgtta
    12721 aaactgttgc atggtatgac aatgagtatg gatttacttg ccaaatgatt cgcttactag
    12781 aaaaattcgc taacttgtaa tttgatagat caatcgataa aagaagcctt tgactggatc
    12841 cagtcaaagg cttctttctt ttcatcccct acttttaaac tcgttcaatg aacatattgg
    12901 cttaaaaatt gttggactac ttgctgatat ttttcaggat ctgtcttgta tgcttccgca
    12961 tgctcggctc cttttacaac atacttttct tttggtccct tcgttgcacg atagacctta
    13021 tctaacattt caaatggtac aaatgtatct tcgtctccat gtataaagag cattgggcgc
    13081 tggtttttct tcaattgttc cactgcattt gcttcaccga aaaagtagcc agcacggact
    13141 tttgtcacta gactcgttac ttgcataagc ggaaatgctg gaagactaaa catatcgtct
    13201 agctgataag caagctcatc atgaacggaa gaataaccgc aatcttcaat gattgctttc
    13261 acattatctg gtagtttttc tccgctagtc atcataactg ttgctgctcc catgctgaca
    13321 ccgtatagaa cgatttcttg cgattttcca ttgttttcta gcactttgtt tatccactgt
    13381 acatagtctt tacgctctgg ccatccaaat ccaatgtaat ccccttcgct ttttccatgt
    13441 cctcttgcat caggaacaag gacattgtaa cccatatcat gaaacatctt tgcgtaattg
    13501 gtcatcgttt cagcgtttcc catatagcca tgagccacaa gagctgtttt ttcacttttc
    13561 gtttcagcag gaagataaat cgctgataat ttcagaccgt cttttgaagt gatggtccaa
    13621 tcgcttcggt tttcttttga actaaaccat ttatgtgcat ccgtttcttt ggatgtctgt
    13681 gttgtaccag gtgtatcttc gtctaaaaag tctttctcag aaggcacaac agcataatta
    13741 taaaaataat tccctgcaaa cactaacgca acaatcagag ctaaagcgac gcctaaaagg
    13801 attttcatcc aaagtttcat aaatcatcca tctcatttct cttttttcat ataaccttga
    13861 attagtataa tcaaagaatc tctactatgc aatgttcatt tttataaccc gcgaattgta
    13921 aaattaagtt agaaaaataa aaaggcattt atggtacact caaattgtat ttccgacgaa
    13981 agaaaacaaa ggagtgtaac cataaatgac ctacaaacat cttaccatag acgaactgac
    14041 aatgatagaa tcatattatc ttcaacataa taagccggtt gaaatcgcta accgaatggg
    14101 acgtgctata caaactattt ataatgtagt caataagttc aagcaaggca agactgctct
    14161 tgattattgg caccagtata aagaaaataa gaaaaaatgt ggtagaaaag tcattcaatt
    14221 acctgctcat gaagtagatt acattaaaga gaaagtcact cttggttgga cgcctgacgt
    14281 cattatcggg cgaaaagaaa ggtctgtttc atgcggtatg agaacacttt atcgtttatt
    14341 ttctaaagga atatttgata ttgacacact accgatgaaa ggtaaaagaa aacccaatgg
    14401 ccatcaggaa aaacggggaa aacaacagta tcagcgctca atccatgatc gacctgataa
    14461 ttatcctgat ttcaattctg agtttggtca ccttgaaggt gatacgatcg ttggcattca
    14521 tcataaaagt gccgtcatta ctttagttga aagattatct aaagtcatta tcacgattaa
    14581 acccaacggc cgtaaggcat tagatattga aactgccctt aatcaatggt tttctcgctt
    14641 ccctaaaaac ttctttaaat ctattacgtt tgactgtgga aaagaatttt ctaactggaa
    14701 agccattagt aaccaacatg atattgatat atattttgcg gaccctggaa caccttctca
    14761 acgcccatta aacgagaatt ctaacgggat tctgcgtcgt aatggactgc cgaaatcaat
    14821 ggattttaga aaagtgaatc agacatttat ttccagtgtc agcaatcaac gtaatcatat
    14881 tccaagaaaa tcattgaatt acagaacacc aatcgaaata tttttgagct atgtacaaga
    14941 agcattttat tctagcttaa tttgacaaat cataacctat aaaaaaacaa aataaaaaag
    15001 cagaaataac tggctatttc tagcggatta tttctgcaat aactaattat ctatttttta
    15061 ttttcaatta atttaaagct tgaccatggt ttcaaaaagt caaggaggaa taattcgtca
    15121 tcggagattc ttcctacaac attgacacgt ccgtcgtttt tcatatcctt caaggcgatt
    15181 tgtgtttctc ctttgtattg accgtaccct tcattgtcaa tcagtacatc cccatgatgc
    15241 atatctcgcg tattatgagg cgggaaatct ttatctttgt agtatacacg agtcatcgtt
    15301 gaacgaagga tatattctga gcgatcccct cgataactat gaaggttatc aaacaagcag
    15361 attcgttcgt tttctgtaat accttcttcc gtatctactt tcaatgttgg ataatccgcg
    15421 ttaaatgctt ctgccatttc tttcaattct tcttcacttg cgtacgcatt tccaatagaa
    15481 atatcatcaa tcaatcctgt aaggattaaa tgcttcactt gtgtcgcaat ttcaagatct
    15541 cggtggtctt ccagcgtgca caagccatct tgcgtaggcc aagggccaaa ctcagcagat
    15601 tgcgaattga cgaatgccat cgtattcaaa ttatattttc tgaatttttc tgaacaaaaa
    15661 acaaagtgtt cgtatcctaa accagagtat cgatgaggat aaaaattatg agaacctaat
    15721 aaattatccg tgtttggcga ataactcatg atgttgtcaa cgtaattcgt gcccgaactc
    15781 atattgatct cgattttgat accaaaagga ttgcgcgtca tttttgcttc ttctgctcct
    15841 gtgaatccga tatctaagcg gaccccgtag gcacccatct tatggaaaaa agataagtca
    15901 tcgtaagaaa tctccaactg ttcaaataac gctggattga tatcaaccat tacttccatg
    15961 cctaggctat tcgcgtaatc aactacttct ttaaattctg aaagaacttt ttctttatca
    16021 tcattgatct gcagcaaact ggtaaaaacc cgtttatacc catacttatg agctaaatcc
    16081 aagtacgctt tgtccttttc aaatgttgac cgttctggat aaatcgagat tcctaatttt
    16141 cccatattac catttcctcc tgcatctttt cgtttttgta tccacttaca ttatagcaaa
    16201 cacaaagtaa atggtctaca ccttttcttt ttcttttaaa ataaatgata aattccaata
    16261 atttaagtag gtattttccc tctattttgt tatcattaaa gataacaaaa tagtaaattg
    16321 gaaaggaaca gttatgaaga aaaataaagt tattttaagt attagtttag cagcagcatt
    16381 gctgtttgct gctggttgtt ctgcaaacaa cacgacttct tctagttcgt ctacagaaag
    16441 ttcaaaaagt acatctactg cggaaagcac gactgccagt agcgtcgatt tgaacgcatt
    16501 ggaattacca caattgacga aagatgtcgc cgaaaatgaa gatctggtcg aaatggtgac
    16561 gaataaaggc acaatcaaaa tcaaattatt tcccgaacac gcaccaaaag cggttgaaaa
    16621 ctttatgaca catgcaaaag acggttacta caacggactg actttccatc gcgtcatcaa
    16681 agatttcatg atccaaggtg gcgatcctaa aggcgatggc actggagggg aaagcatttg
    16741 gggagaagga tttgaaacag agatcaacaa ccacctttat aatataagag gtgcgttatc
    16801 tatggctcgc tcacaagatc ctaacagtaa cggcagccag ttctttatcg ttcaaaatac
    16861 agatgatatg catgatggtc ttttaaaaga cgactatcct caagcaatca tcgacgctta
    16921 taaaaacggc ggttatcctt cactagatgg ccattatact gtcttcggcc aagtgattga
    16981 aggaatggat gtcgtggatg ctattgcaaa tatcgaaaca gatagttcag acaaaccaaa
    17041 agaagaagta aaaatcgata aaatcaatat actgcaagaa gcaaaacaaa aataaaaaaa
    17101 taacgtctga atcctgcata gatgacaacg tatgctagga ttcaggcgtc ttctttttac
    17161 ataaaaagca agtaagcttc cttttgtttt gctatatcag gttttatagt caagcatttt
    17221 ggttgacgat gaacgtgaac tcgcactcac agactttttt atcatctaca tatgctgcag
    17281 ataacgctgt accgactgat cccttcattt tggtgatttc aaaatgacat ttcagcacat
    17341 ctcctggaac aactttttga cggaatttgg ccttattgat cccaccgata taagctcttt
    17401 ttcctagaaa ctcttctgat tttaggataa gtatcgaacc tgcttgtgcc agtgtttcta
    17461 gaatcaacgc acccggcatc actggattgt caggaaagtg accttggaaa aaatcttcat
    17521 tgattgttac attttttgtc gcaacgatct tttttcctgg ttctaaagta tctacataat
    17581 caatataaca aataggataa cgattaggaa tcaattccat tacttcagta gcagataata
    17641 cgtaagacat aattaaactc cttctttcag gcaattgcca ttttcattac tttgactatc
    17701 aaagtatcat attcctaaat atttagcaat ctttttttaa tttaagaaaa aatagtttga
    17761 caatcaaaac attttgacta ataattaaga aatcaagaag ttattttagc tattattctc
    17821 tattttttta cgactatcaa atttttatca gaaacaaagg agagtcatta tgtcatttct
    17881 agaaggaaaa aaaatcgtca ttatgggcgt cgctaacaaa aaaagcattg cttggggttg
    17941 tgctgaagca atgttgaaac aaggggcaga aattatctat acttaccaaa atgaacgaat
    18001 gaaaaaatca ttagtcaaat tggtcggcga agaagcattc actgtagaat gtgatgtttc
    18061 tagcgacgaa agcatcgaaa aagcaatgaa tgccatcggt gaatatgctg gggagatcca
    18121 cggccttgtc catgcagttg cttacgccaa caaagaagaa ttgtccggaa atgtgtctga
    18181 tatttccaga gacggctatc aattagctca agatatcagc agctactctt taattgctgt
    18241 atcaaaatac gcacaaccgt ttcttgcacc aaacagcggg atcgttagca tgacttatct
    18301 aggttcagaa cgcgcaatcc cgaattataa catgatgggt attgcgaaag cttctcttga
    18361 agctgctatc cgttatttgg cagctgagtt ctcgcctaaa ggaattcggg tcaatggaat
    18421 ctcagctggg gcaatcaaaa cattagctgt cacaggtgtt aaagactatc aaaaactgat
    18481 ccagttatct gaatctcgta cgcctgatca gcaaggtgtg acgatcgaag aagtcggtaa
    18541 tgcttgtgct ttcttaatca gcccactttc cagcggaatc atcggtgata ttatctacgt
    18601 tgacaaaggt gtccatcttt cataaacttt tgttgcattc tttaaagtaa aacatataaa
    18661 aatcaggaaa aaacttttta aagtctcttc ctgatttttt ctttttacgt ttttttcaac
    18721 tcattacttt gaaattattc cacgacttca atccagcctt ctggtgcttc tatgtctcca
    18781 aattgaatac ctgtcaattc atcatacaac cgttttgtta ctggtcctac ttccgtttca
    18841 ctataaaata catgaagatt gttattgtga taaatcccac cgattggaga aataacagcc
    18901 gctgtaccac atgcaccagc ttctgcaaat tgatctagat catcaatgaa aatatctcct
    18961 tcaattgctt gcagtcctag tcgttcttca gctaactgca acaaggaata tttcgtgata
    19021 cttggtaaga tagagggcga ttgcggagtg atgaattttc cgtcttttgt aattccaaag
    19081 aaatttgctg cacctacttc ttcgattttc gtatgtgtgg ctggatcaag ataaacacaa
    19141 tctgagtaat ccagttcttt tgcttctgtt cctggaagta aactcgctgc gtaatttccg
    19201 ccgacttttg ctgcaccagt ccccttagga gcagcacgat cgtaatcaga tacaataaaa
    19261 ttcgttggtt ttagtccacc tttgaaatac gctcctactg gcgtacagaa gacgacaaat
    19321 aaatattctg gagctggatg gacaccaatg ttttcaccga ccccaatcag taaagggcgc
    19381 aagtataatg tagcgcctgt tccatagggc ggcacaaaat cttcatttgc ttttaccact
    19441 tgcttgacag catcaacgaa catcttttca gggaccattg gcatcagcaa tcgtctagca
    19501 ctttgattca tccgtcgact attttgttca ggtcgaaaga gattgacctt gccgtctttt
    19561 cgacgataag cttttagacc ttcaaaacat tgctgaccat aatgaagcgc tggagagcct
    19621 tcatgaatcg tcaggtaatt atcttctgtc aattctcctt cttcccattg tccgtctttc
    19681 catttggcaa taaaacgcca aggagttttg atataagaaa aacctagatt gttccaatcg
    19741 atttgcatat acttaccacc ttcgttattt tctccacatt ttatcattat ccatattatt
    19801 tgtcatttta ttttcagctt ttttccttca ttatagttat ttttgctatt tccttttttg
    19861 aaaacttctt ggtacaatac agaaagaatc aaattttaga aaagaggatt ttatgttttc
    19921 attattagca actgccggta cgacagactc ttctggcgag acattgacag atcaagctgt
    19981 cagcaaagta aatgcgtttc agcggttttg ggacgggatt gattgggacg ccattttggc
    20041 tgcctttatc caaaaaagtt tatatctgat ttttttgatc gtcctttttt ggattcttca
    20101 ccgtgtggga aaatatctga ttgataagtc ttttaatcag tattctaaaa aagccattct
    20161 aaacgagagc cgcttaaaga ctttacatag tcttttaaat actatttttc attataccct
    20221 tggtttcttt tttatctatg caattttatc tgctatcggt gtgccgatcg gatcactttt
    20281 ggctggtgcc ggaattgcag gtgtggcaat cggtcttggt gcccaagggt tcatgagtga
    20341 tgttattacc ggatttttta ttatcatgga acaacagatg gatgttgggg actacatcaa
    20401 gctagcaaac ttaacgattg aaggaaccgt cgtatctgtt ggtatccgta cccttcagct
    20461 gaaagcaacg gatggtactg tccatttcat cccgaatcga aacatcacca cgatcagcaa
    20521 tctctctcgt gcaaatatgc aagtactttt agatattcgg attgttcctg aagaaggata
    20581 tgacaaaatc tatgagatca ttgatcgagt aaatcgtaca ttagctgaaa aataccaaga
    20641 agagctccaa actgaacctt ctatctttgg attagtggat cttggaaaca gcaattttgc
    20701 gattcgtacg gtctgctacg cattgaacgg aaaacaatat gccttaaaag aagagttctt
    20761 gagctcctat gtcaaagaat tgacagcttc tggtttcacg attccgaaca gtccaattgc
    20821 tgtcggaaaa taatttggac aaacgatgca acgaatctca ctaaaaagag cgtgggacaa
    20881 aagtaaagaa cacttttgtc ccacgctcta aacacgtata aacggcggaa gcagaagcaa
    20941 ctccttcgga aataagctga a
//
LOCUS       DAHUVV010000045        20897 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.045, whole genome shotgun sequence.
ACCESSION   DAHUVV010000045 DAHUVV010000000
VERSION     DAHUVV010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.045"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            598..1287
                     /locus_tag="NK767_001608"
     CDS             598..1287
                     /locus_tag="NK767_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6694606.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1274..2482
                     /locus_tag="NK767_001609"
     CDS             1274..2482
                     /locus_tag="NK767_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCH6694607.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2776..4047
                     /gene="serS"
                     /locus_tag="NK767_001610"
     CDS             2776..4047
                     /gene="serS"
                     /locus_tag="NK767_001610"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCH6694608.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="NK767_001611"
     CDS             complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="NK767_001611"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCH6694609.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6585..7280)
                     /locus_tag="NK767_001612"
     CDS             complement(6585..7280)
                     /locus_tag="NK767_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HCH6694610.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="NK767_001613"
     CDS             complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="NK767_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCH6694611.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8479..8670)
                     /locus_tag="NK767_001614"
     CDS             complement(8479..8670)
                     /locus_tag="NK767_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCH6694612.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8691..9581)
                     /locus_tag="NK767_001615"
     CDS             complement(8691..9581)
                     /locus_tag="NK767_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCH6694613.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9568..10335)
                     /locus_tag="NK767_001616"
     CDS             complement(9568..10335)
                     /locus_tag="NK767_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCH6694614.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="NK767_001617"
     CDS             complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="NK767_001617"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCH6694615.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="NK767_001618"
     CDS             complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="NK767_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCH6694616.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="NK767_001619"
     CDS             complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="NK767_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCH6694617.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14906..15289
                     /locus_tag="NK767_001620"
     CDS             14906..15289
                     /locus_tag="NK767_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694618.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15457..16215)
                     /locus_tag="NK767_001621"
     CDS             complement(15457..16215)
                     /locus_tag="NK767_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HCH6694619.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16233..17051)
                     /locus_tag="NK767_001622"
     CDS             complement(16233..17051)
                     /locus_tag="NK767_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCH6694620.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="NK767_001623"
     CDS             complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="NK767_001623"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCH6694621.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="NK767_001624"
     CDS             complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="NK767_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCH6694622.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18179..19513
                     /gene="dnaA"
                     /locus_tag="NK767_001625"
     CDS             18179..19513
                     /gene="dnaA"
                     /locus_tag="NK767_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCH6694623.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19711..20841
                     /gene="dnaN"
                     /locus_tag="NK767_001626"
     CDS             19711..20841
                     /gene="dnaN"
                     /locus_tag="NK767_001626"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCH6694624.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5975 a         4255 c         3831 g         6836 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggctgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt tttcgaaatc
      121 ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact tttttgaagt
      181 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
      241 atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct
      301 tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt
      361 atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta
      421 taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat
      481 tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct
      541 atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg
      601 aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac
      661 aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga
      721 acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg
      781 caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa
      841 actactgata tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag
      901 ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg
      961 cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac
     1021 tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt
     1081 atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag
     1141 cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat
     1201 ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga
     1261 gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa
     1321 tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt
     1381 gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc
     1441 tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt
     1501 gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt
     1561 gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact
     1621 tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt
     1681 aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga
     1741 cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg
     1801 taccccactc acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc
     1861 agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc
     1921 tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt
     1981 ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc
     2041 ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg
     2101 cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg
     2161 aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac
     2221 agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt
     2281 ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat
     2341 cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg
     2401 gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca
     2461 ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg
     2521 aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc
     2581 agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc
     2641 ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc
     2701 gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa
     2761 aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa
     2821 gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat
     2881 gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt
     2941 tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa
     3001 atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa
     3061 aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga
     3121 acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt
     3181 gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc
     3241 gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt
     3301 gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc
     3361 actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa
     3421 gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca
     3481 ttaactaact attataatga tgaaatttta gatggtgcag atttacctat ttatttcact
     3541 gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg
     3601 attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct
     3661 tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg
     3721 ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat
     3781 gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac
     3841 tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc
     3901 cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt
     3961 ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat
     4021 atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta
     4081 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg
     4141 agaaactggc ttttgaacac catttatcga gaggttgtga aatcagcgaa ttaacctgag
     4201 tattagagaa aaaaatggaa aaatagtccc tcatatttta ccgtttgttg aactaatacc
     4261 gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc cccagctcct
     4321 agttacaagg acaaattcaa aaaacagctc ttcatatttt atgaatgttg tacttgtgat
     4381 tcggaggtgg caagggaaca ctgtttattc aaaaagaggc tgggacagaa gcgtttaact
     4441 ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcttctta
     4501 tttccgaagg agttgcttct acttccgccg tttatacgtg tttagagcgt gagacaaaag
     4561 tgttctttac ttttgtccca cgctctttaa tgtttttaag attcaactga atagtttggt
     4621 gcttcttttg tgatttgtac atcatgaggg tgtgattctt ttaatccact gccgctcatt
     4681 tggataaatt gcgcttcatc acgtaattgc tgtagattgg ctgccccaac ataacccatt
     4741 cctgaacgca aaccgccgat catttggaaa ataatatctg caacacttcc tttataagca
     4801 acccgacctt cgattccttc tggtactaat ttatttgctt cattgacact accttggaag
     4861 taacggtctt ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg gtatgttttg
     4921 aatcgacgtc cttggaagat ttcgaattca cctggtgatt catctgtccc tgccaacata
     4981 cttcctagca tcactgcatg tccgccggca gctaatgctt tgacgatgtc accagaatat
     5041 ttgatccctc cgtctgcaat aatcgctttg ccatattgac gagcaactga tgctgcatca
     5101 taaatagctg tgagctgagg tacacctaca ccggctacca cacgtgtcgt acaaatcgat
     5161 cctggtccga taccaacttt tacgacatct acacctgcat catataaagc ttttgtcgct
     5221 tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg caaaagttga acggatttct
     5281 tgaatttttc ggatcacacc tgcactatgt ccatgagctg tatcgatgat gattgcatct
     5341 gcccctgctt ctaataaagc attcgcacgc tcaaatgtat cgcttgttac accaacggct
     5401 gctgctacaa gcaatcgacc atgttcatct tttgctgcat ttggaaattc gatcactttc
     5461 tcgatgtctt tgatcgtgat caatccgctt aaacgacctt cgttatctac gattggtaat
     5521 ttttcgattt tatgtttttg caaaatcttt tctgcatctt ttagagaggt acctactggg
     5581 gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata atctgttaca
     5641 aaacgcatgt cgcggtttgt aattatccct accaattttc tgttttcaag tgtttcaacg
     5701 attggcacac cactgatacg atatttgctc atcaattctt ctgcgtctgc tacaagatgc
     5761 tgtggcgtta agaagaacgg atcaatgatt acgccacttt cagaacgttt gacttttctt
     5821 acttcatccg cttgctggct gatcgtcatg tttttatgga tcacacctag tccgccttgt
     5881 cttgccatag caatggccat cttgctgtcg gtaacagtat ccatacttgc actgatgatt
     5941 ggaatattca acttaatatt tttagctaat tgtacgctca tatccacttc gtttggtaac
     6001 acgtgacttt ctgctggtat caataagaca tcatcaaaag taaatccttt tttcgtgaac
     6061 tttgtttccc agttggacat ttttagtacc actcctctaa tttagtttat ctattaaaat
     6121 aattgatttt ttcatttaag tcaatgatct acttggaatc aagttagctt tctcatagaa
     6181 taccaatata aagcgtttac atctgtgtgt attaaaagag attgtaaaaa agctgtcctg
     6241 catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgaa ttggttgggt
     6301 ttattaccgc agatgcaaac ttttgaacac cgtttagtgg agaggctgtg aaaaagctgc
     6361 ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
     6421 ggacttaata ccgagaaact ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca
     6481 agacttttgt cacagtccct tactattatt taactatgga taaacaaaat gaggttcttt
     6541 actaccccta gatatctagc aaaatagctg atctttttgc tagtttatct tcttgggacg
     6601 gataaacttc caataatccc gtattttttc ttcaagtagt aacggattgc taaagcagtt
     6661 gccccgataa tgataactac tactggatct aatactggat tgataactgg tggcaatagt
     6721 gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc cacctaggat aagcaatgtc
     6781 ttacccattc ctggtttctt gcttcgatct gctccgggat attcatattg atagatgtat
     6841 ttgtacatca tataaaagac ccagccaccg attgcagaac ctagaacgag tgtagaaaag
     6901 ccataagctg gcgttgttcc acgtgaaaac aatccggtaa tcgcaatcat gatacttaat
     6961 agaccaagca acagcaacgt attatctaac cacatcaata aaggggtgga tgaatttttt
     7021 acttctgctg gcttattgat gattgcttcc gttctttcag agactgtccc aaacaattga
     7081 cgggctgttc ttccgccttt ttgttctgca actaaagtag gcagaatgtc atgaagggct
     7141 tttacttttt cttcttcaga caaattcgct gcttctaaag attttttcaa atcaaaaatg
     7201 tactgttgat ttcttttggt taattttgtt tctaaactat tgttttcttt tacgattgct
     7261 cgtaattgtt ctggttccat tatctaatag tcctccttag ccgttccttt tctcaacggt
     7321 cgtcacaaaa gacattctac catattctac agctagtttg atagcccttt tacacattaa
     7381 agcggaataa cattacgtcg ccgtcttgta cgacataatc ttttccttct aatcgaacac
     7441 gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc caagtcttca aaagagacag
     7501 tttctgcacg gataaatccg cgttcgaaat ctgaatggat gattcctgct gcttgtggtg
     7561 ctttgattcc ttttttgaat gtccaagcac gtacttcttg ttccccagca gtaaagtaag
     7621 tagctaagcc taacaaatca taagcagcac ggatcaactg atccaaacct gattcttcga
     7681 ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc taattctgcg atttcttctt
     7741 ctgcacgagc actgaccacg atcacttctg cattttcttc tgcagcaaag ttgcgcactt
     7801 cttgtacata atgattcgcg tctgcatctg ctacgtcatc ttctgagaca ttggctacat
     7861 aaagaattgg ttttgtcgtc aataaaaata gtgatttaac gattttttgt tcttcttcag
     7921 taaattcgat cgttcgggca gattttcctt cttccagtac aggtttgatt ttatccagta
     7981 cagctagttc tgccactgca tctttatctt tggttttagc aacttttgct acacgagtat
     8041 agcgtttgtt gatagattct aagtctgcta acactagttc caagttgatc gtatcaatat
     8101 cttctagtgg atcaacacgt ccttctacgt gagtaatatt atcatcatca aaacagcgaa
     8161 caacgtgaca aatagcgtct acttggcgga tatggcttaa gaattgattt cccaaacctt
     8221 ctcctttact tgcgcctttt acgattccag caatatctgt aaattcaaaa gtcgtcggaa
     8281 cagttttttt cggatgtaca agttctgtta agcgttgcaa acgccagtca ggtacttcta
     8341 ccatccctac gttggggtcg atcgtcgcaa agggatagtt tgctgcttct gctcctgctt
     8401 ttgtgatcgc attaaacaaa gttgattttc ctacgttggg caacccaaca attccagcag
     8461 tcaatgccat aaatttattc actctttctc tgcatttttt tcaattactt tttttaattt
     8521 cttttcaaaa tctcgacggg tcatcatgac gatatgtccg caattcatac atctgatttt
     8581 gatatctgcg cccattcgaa tgacttccca gcgattcgtc tggcaagcgt gaggtttttt
     8641 catttcaacg atatcgtgca agtcgtacat aaaaatagcc tcctttttgt ttattcttca
     8701 tcaaattgaa tattcaagat atcaagaatt cgagtcaaat ctttttgaga aagatattca
     8761 atctcgatct tacctttccc atttttttct tggatagcta cacttgtacc aaatttatcc
     8821 atcaaacgat cttcgctttc tcggatataa tatggttttt cctttgctag ccttgggatt
     8881 tttttctttt cttttccatc attttgattt aactcagaaa caagctgttc cagttggcgg
     8941 acagttaaat tttcagttac tgcacgattg gctaatttca agatcaatgt tttatttttt
     9001 aaacctaaca gcgtacgggc ttgtcccata gataaacgct gatcttgtac catttccttg
     9061 acaagatccg gtaaagaaag caagcgcaaa taattggcga tataaggtct gctctttcct
     9121 aaacgactag ctacttctgc ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct
     9181 tctgcttctt ctaacggatt cagatcttct cgctgcaagt tttccagcac tgctacttgc
     9241 atcatcgctt cttcgtcaaa gtctctaacg atagcaggga tcgtttcttt ttcagctagt
     9301 ttggacgctc gaaaacgtcg ctcccctgca ataatctcat atccttttac tgcggaacgg
     9361 cgtacgatga tgggttgaaa gacacctgat tgttcaatag aattagctag ttcctgcaaa
     9421 gatacttctt caaatgtttt acggggctga tacggatttg gtcgcagctc attcaatggt
     9481 agctggacaa cggtatcatc ttttacatcc accgtctcta agttttctaa atcttgaaac
     9541 agtgcatcga ttcctcggcc taatccttta cttttcttca cgacttacca cttcctttgc
     9601 caatgcttgg tagacttcag ctcctcgaga acgtggatcg tagtcgatga tcggttttcc
     9661 atgactaggt gcttctgata aacgaatatt tcttggaatg atcgtttcgt aaactttttc
     9721 gcggaagtat ttacgtactt cttctacgac ttcatttccc agatttgttc gtgcatcata
     9781 cattgtcagt aagactccct cgatctctaa ttctggatta aagtgttttt ggactaaacg
     9841 aaccgtattc agtaattggc ttaatccttc caatgcgtaa tactcacact gaacaggtat
     9901 caaaatcgaa tcacttgctg taaatgaatt gattgtcaga tggccaagag aaggcgggca
     9961 atcgatcaaa atataatcat actgactgct tacttctgct aaagatcctt ttaatcttga
    10021 ttctcttgcc atcattgacg tcaattcgat ctctgctcct gctaattgga gagtcgcagg
    10081 cacaatactc aaattttcgt gttcagtgat caaagttgcc tcatcaattg gcaattcatt
    10141 gactagaaca tcatagatgt ctcttgtcac atcaggtttt ctgatcccga caccactggt
    10201 agcatttcct tgtgcatcca tatccactag caatactttt ttgccaaggc tagcaagaca
    10261 agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca cctttttggt tcgcaacaga
    10321 tattatttgt gccatctctt ctcctcctat ttgattggct gcttattagg cagtccgggc
    10381 tttcttgggt atttctttgg ggttggtttc tttttttgaa taacgatgat atgccgttga
    10441 tcagagacat tcggcaaggt aaccgcctct tctttgagga atttaccacc taataaagca
    10501 atcgcttgtt ttgcttctgt caattcctct tcactttttg ctgctttcaa tgcataaaaa
    10561 tagccttctt gtttagccaa gggcaaacac aattccgcta acacattcaa acgagcaacc
    10621 gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag gattttgccc aaatgtttct
    10681 gcccggtcat gatataagga agcatcgatt cctaataatc gggttaactc agataagaag
    10741 gttattcgtt tattcagaga atcaacgatc gttatctgca gttctggaaa ggcaattttt
    10801 aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc ataaagataa aggttgcgta
    10861 aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt
    10921 tcatccgtaa tagctgtaag gttcatcttt tcgttccatt cgactaatgt ccgataataa
    10981 atagcgaatt gattcatttg atgatcggta agctctatcc ctttttctgc caaggattgg
    11041 cgaaattctt ctggtatcat aaatattcct ttcattgtga aacagcaatt gtttcacaga
    11101 ttctctccct actttaacgc aaaatctcta gacatgcaat ccttccatat ggattcttaa
    11161 aaatttgaaa agtaagaaaa gccgatagac tttttctttc tagtatgact cataaaatat
    11221 acacagaaga gagaactagc tttgagcaat ttctgcaaaa actagttctc tcttctattt
    11281 attattacgt attacgctgg ttcactgact ttcgcaatct tcccttgttc gatatagacc
    11341 attaggatac ttaggtctgc agggtttacg ccactgattc ggctagcttg ggcaattgtt
    11401 tctggttgga ttttctgcaa tttttgtttt gcttctgttg ctaatccgtt gattgctgcg
    11461 tagtcgatgt tttctggaat ctttttcgct tccatgcgtt ttaatttttc tactttttcc
    11521 atggcttttt tgatgtagcc ttcatatttg atttggattt ctacttgttc gatttctttt
    11581 cgtgtcaatt cttcattttc tggaataaac cgtagaagtt ctgcataagt aatctcagga
    11641 cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg gtgccgcatt aatcgttgct
    11701 aaaaagtctt ggacttcttc tgttggtttg attcgtacat tcatcaaacg tttgatttct
    11761 tgttcaactg cttctttttt cttcaaataa ttcttgtatt gatcttcttt tactaagcct
    11821 acacggtgac ccatttctgt taatcgcaaa tctgcattat catgacgtaa aagcagacgg
    11881 tattcagcac gtgaagtcag caaacgataa ggttcgttcg ttccttttgt taccaaatca
    11941 tcgatcatga caccgatgta accgtcactt cgtttgatca ctaaaggttc ttttccctga
    12001 actttcaaag ctgcattgat accggcaatc agcccttgtc cagccgcttc ttcataaccg
    12061 cttgtaccat ttgtttgtcc agctgtataa agattttcaa cgactttagt ttctaatgtc
    12121 ggacggagct ggtgtggctc tactacgtca tattcgatag catatcctgt acgcatcatt
    12181 tctacttttt ctaatccagc aatagaatga agcatgttga cttgaacatc ctctggtaaa
    12241 gaagtagata aaccttgaac atagacttct tccgtattta agccttctgg ttccaaaaat
    12301 aattgatgac gtggtttgtc tgcaaaacgc acgatcttat cttcgatgga tggacaatag
    12361 cgagcgccta ccccttcaac gatccctgta aacattgggg cacgatgtaa gttgtctcga
    12421 ataatttggt gagtcgtctc acctgtatag gtcagccagc aagattcctg atctagttta
    12481 taggcactat ctggtgtgct gaagctaaaa tgattcggct ctttgtcacc aggctgctct
    12541 tctgtaacag aatagtcgat cgtacttgat tttactcgag gcggtgttcc tgttttgaac
    12601 cgttcgatag caaagccaag ttcttttaga tgattcgcta gtccaataga aggttgagag
    12661 ttattcggac ctgatgaata tttcaattca ccgataatga tttctccgcg aagtgctgtt
    12721 ccggctgtaa taacaactgc tttggcccgg tagctcgctc ctgtggacgt cactactcca
    12781 tgacaaacac catcttcgac gatcagttct tcgacgattc cttgtcttag cgtcaaattt
    12841 ggttcttttt cgatcgtatg tttcatttct gttgaataag catgtttgtc tgcttgtgct
    12901 cgcaatgctc ggacagctgg gccttttcct gtgttcaaca tacgcatttg gatataggtt
    12961 ttatcgatat tcttacccat ttctccgcct aacgcatcga tttctcgaac gacaacgcct
    13021 ttagctggtc cgcctacaga aggattacat ggcatgaatg ctaccatgtc caaatttatt
    13081 gtcagtaata atgtttttga tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg
    13141 tgtcctgcgc caacaacgat cacgtcatac gcatctgctt cataatgttc caatcgtttg
    13201 ttcctccttt tattttccta aacaaaattg actgaataat tgagtgatta gctcatcttg
    13261 tacactgtcg ccgacaactt ctcctagata gtcccagcaa cgcgtcatat ctatttgaac
    13321 gagatccaca ggcattcctg aatcaatacc tgcaatgact tcttcaagtg agagggaagc
    13381 tttttctaac aacgcgatat ggcgggtatt agaaacataa gtagcgtctc tttctcctgt
    13441 ttctccgctg aagaataaat cagaaatagc tgattccaaa cgatctagtc cgtcgttttt
    13501 agctacggaa attgaaaaaa ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc
    13561 cagctgtgct ggcaaatctg ttttatttag aagaatgatc cgtttcaatc cacttgtcgc
    13621 ttccaataat tgttcatctt ctgctgtcag tggttcgctt tggtttagaa caagtaagat
    13681 caagtctgct tctgctaatg cttttcggct tctttcgacc ccgatccgtt ccaccacatc
    13741 ttctgtttcc cgaattccag ctgtatcgat tagcttgagc ggtacccccc gaacattgac
    13801 atactcttcg attacatctc gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc
    13861 tcttaacaaa tggtttaaca aacttgattt gccgacattt ggccggccga tgattgccgt
    13921 acttaaccct tcccgcagaa ccttcccttg cttggatgta gcaagtagcg cttggattct
    13981 ttgttggatc atctgcgctt tttcaagcaa taacttcgta gtcaattctt cgacatcatc
    14041 atattctgga taatcaatat ttacttctac ttgtgctaat gtctctaaaa tctcttgtct
    14101 caatgagcgg atcaaagcag acagattgcc gtctaactga ttaagagcta gtcccattgc
    14161 tttgtctgtt tttgcccgaa tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg
    14221 accattcaag aatgctcgtt ttgtgaattc gcctggctca gctaatcttg caccttctct
    14281 taacaatagc tgcaaaattt gattgactac aacgatccca ccgtgacaat tgatttctac
    14341 tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg gagaccatga cttcatcgac
    14401 aagctgattg ctcttgggat caacgatatg tccataatgg atcgtatgac ttggtacttc
    14461 tgataatcgt ttgtttccag attgataaac tttatctgct agttgcacag cttgatcgcc
    14521 acttaaacga acgatactga ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt
    14581 atcaaattct aaagtaattt cagccatttt ttcacttcct ttttcatttt gcgcacaaaa
    14641 aaagtgccca ctccaccctt tatgcacaat aaacggtgaa cttcgcactt tgactgcttg
    14701 ttcttgatta aattctggtc atagattatc acacttgcta taaggaatca agtgtttaaa
    14761 acaattagat gaagttgagc ctgttctgct tccattacct tatatttaat tgtccttgga
    14821 tgtctctttg cgttctttgg tttattcgaa gagaagatac tgtataaaca aggctttatc
    14881 tttcttaaaa aaaacggtac actaaatggt agcgctatct aaactataca cttttgaaag
    14941 gagaaaatat gtgagcccgt tcagatctgc tgcattggaa gaagaaagtg accctcggat
    15001 caacgatgtc gttttatcta cagggaatat caaccagaaa tatatcgtcc gcgatatcgt
    15061 ttttgttgtg gaatgcttag aagctgattt gtttgaccca tcttttccca aagatgacct
    15121 tttcttaaca acgaagagga aattaaagac gaaggtactt gaatatggtg cgaatgcagt
    15181 aatcaactgt cattttgagt atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact
    15241 ttttgcttat ggaacagtga tccaatttat ccaaacaacg attggttaaa tgaaacagac
    15301 aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct tttttctatc aaagttagtg
    15361 actacttcat cgtatactct gataagaatg atttctagta aaatatactt ggtttgaaaa
    15421 actttgtcaa cgtgatttat tcaaaaagta atggaatcaa taatattttt tagcaggttc
    15481 tactactaaa taacgataag gttcctcacc ttccgaatgt gtttttacat gttcatcttt
    15541 gcttaatgca gcatggattt gtttacgttc aaatgcaggc attggttcta agaaaacagg
    15601 ctgccctgtc cgttttactt tttcagccgt gcgatcagct aaacgctgaa tgatctcttg
    15661 gcgcttttca cggtaattgc ctacattgac tactacagac agcttatttc ttgctaaacg
    15721 atggatgaag acttgtgcca gatattgcaa tgcattcagt gtttttccat gtttcccaat
    15781 cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc atattatttg attctctttc
    15841 cattttgaca agagctggag catcaagctg ttttgaaatt tcagtaagat aaactgccaa
    15901 ttccgttaaa gctgcttcat cacctagctc acttacttct tctgtttttt cttcaagaat
    15961 cgtttcgttg actacctcga tctcagctgt tggttcttct tccaaaattt cttctacagt
    16021 ctgttctact gtttcagaaa ctttttcgct gatacttggc tggatcgaaa gccgtgcatt
    16081 ctttcgaccc attcctagga aacctttttt tccttcttcc agtatttcga tttcgacttg
    16141 gtctttggcc aaacccaaat cagccagacc tagttggaca gcttcgtcta ttgttgcagc
    16201 ttcataaatc ggcattagca ttacctcccc tattatttcc gttttttctt aggattcatt
    16261 gctttcttaa gagcacgttc tctttcgcgg attctccgtg cttcttcttc acgctctttt
    16321 cgaattttga aagggttgtt gatcagcagc gtttgtacaa cttggaaggc attggaaact
    16381 acccagtata atgaaagacc gctggctaaa ttgacaccca tcactaaaat cattacaggc
    16441 atcacgaaat tcatgatctt cattgatgca ttcgattcaa tttgactcat gctagataaa
    16501 tacgtacttg caaaagtaaa gattgctgct aaaatcggca aaataaatgt tggatccgcg
    16561 ttccctaagt tcaaccataa gaaatgccct tgtttcaact ctggtacacg tgaaatagat
    16621 tgccataacg ccatcaagat tggcatttgt actaataaag gaagacaacc agcatatgga
    16681 ttgacgccat tttctgcata taaacgctgc tgctcttctc ttaacttact ttgtgtttcc
    16741 ggatctcttg aactatattg ttgctgcagt gcttttaatt tcggttgtaa ttcttgcgtt
    16801 ttacgcatgc tctttgtttg gaaatgcatc aacggcaata ggatcacgcg gatgatcagt
    16861 gtaaataaaa tgatccctat ccctgcatta ccaaatgaca acgccttgat cgcctcagca
    16921 aagtaataaa caatataacg atcccaaatt cctgtactct gtgcgctgac ttccccagtt
    16981 ccgcaaccgg ataagacgat gaccaaacta acgataccgg ccatcaaaag taaacgtttg
    17041 tatttcttca cttactcttt tcctctctgt cgattatttt tgcaagtttt aagacgtgga
    17101 ctaaattgga gcgaacctca tcataagtca accccttcac acttggtcta gcaatcacaa
    17161 taaaatccaa ctccggatca atttcttctt ttatagaata aactgctgct cggatttttc
    17221 gtttgacttc attgcggttt accgcgttgc cgaccttttt tcctacagat aaaccaactc
    17281 gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac gaatttgcgg ttagcaaaag
    17341 aatctcccgt atggaacacg gcttgaaact ctcgttcttt ttttactcga tacgcttttc
    17401 tcattagttt tctttccttt tctatctctc acttacgctc ataatcatac cataattacg
    17461 attgtccttt catcatttcc ctaaaaaaga gtataaaaaa aaccactgaa accccagtgg
    17521 cctaagctga aagaactttt cttcctttgc gacgacggct agctaacaca cgacgtccat
    17581 ttttagtact catacgttta cggaatccgt gaactttttg acgtttacgt ttatttggtt
    17641 ggtatgttct tttcatatat tccacctcca aaatgttctt atctatttta taaacacaga
    17701 catactttga tagtataacg acaaagtagt gattttgtca atataaaata gaagatcgat
    17761 tgttaaaact tatccacaag attatttttt tctatgggaa tcctatgtgg aaaaagttat
    17821 gcacagggat tatccacaga taataaaagg ctggttttac acagaaaaca cacgtgtaaa
    17881 aaaagtaatc cacaatcagg cgatttcctg tgtattccgt ctcctaacta ttggtatatc
    17941 tgtatttttt atccacagac gcaagaggaa atctattggt ttttacgagt tttccacctg
    18001 tgcattagtg gtgtggaaaa ctttttttga caactgtgtt tttctctttt ttctttttgt
    18061 cgattgtgtg gataattgta attaaaaaat ttttttcttc ggtttctgtg gaaaactatt
    18121 gaaaaaaatg ctaaattagt actagttatt cactttttaa tgaaggagga ggccttatat
    18181 ggtatccctc gatgctttat ggaatgaatt aaaagcaaca taccaaaagg atttatcgcc
    18241 tgctagctat aatacatgga tcgaaacagc gaaccctcga acccttgatc aaaaccagct
    18301 cgttgttgag gttcctagca agattcataa agaatattgg gaaaaaaacc tggcgacaaa
    18361 aatcgttgaa gtcggttata tgttatcagg aaatgagatt atccctcgct ttattactgg
    18421 cgaagaagca gagcaagaag aagttataga agaaaaaaat ccaaaagttg tggcacctag
    18481 tccgctaaaa aaagccatgc tgaaccctaa atataccttt gatacgtttg tcattggtaa
    18541 aggaaaccag atggctcatg cagcagcgct cgttgtagca gaagatccag gttctattta
    18601 taacccgctc ttcttttatg gaggcgtggg acttggtaag actcacttga tgcatgcaat
    18661 tggtcatcaa atgctgcaaa gtcagcctaa tgccaaagta aaatatgtaa gcagtgaaac
    18721 ttttgctaat gatttcatca attcgatcca aaacaaaaca gcagaagagt tccgtcaaga
    18781 atatcgaaat gttgatttac tgctagtgga tgacattcaa ttttttgcag aaaaagaagc
    18841 tacgcaagaa gagtttttcc atactttcaa tgccttgtac aatgaaggga aacaaatcgt
    18901 actaaccagt gatcggttgc caaacgagat tccgaaactg caagaacgcc ttgtttctcg
    18961 ctttgcatgg gggctgtctg ttgatattac accgccggat cttgaaacaa gaacagcgat
    19021 tttacgtaaa aaagcggcgg ctgaacgtct agagatccca gatgacacac taagctacat
    19081 cgcaggacaa attgattcta acattcgtga attagaaggt gcgcttgttc gagtgcaagc
    19141 ttttgctgcg atgaatagtg aagatatttc gactagtcta gctgcagacg cactgaaaac
    19201 gttaaaatca ggaaaaggac atcctcaatt gtcaatcctg cagatccaag aagaagtggc
    19261 aaagtattat cacatccaat tgaaagatct caaaggaaag aaacgcgtca aatctattgt
    19321 tgttcctaga caaatcgcta tgtatttggc acgtgagctc acagataatt ctttaccgaa
    19381 aatcggagct gaattcggtg gcaaggatca tacaacggtt atccatgctc atgaaaaaat
    19441 ccagcagctg atggattcta gtgtcagtat gcaaaatgaa gtatcagaaa tcaaaaactt
    19501 tttactaaat taatttgtcg attctatcaa ctgaaaatag catgtggata aaagaaaaaa
    19561 acgttcaaaa gttatacaca ctttttccac agctgttttt tcgtttctct cttacgtttt
    19621 ttgagttttc cacagaatca acatgcccta ttacttttat tattcttttt aactaataat
    19681 aaataaacaa acattcaaag gagatacatc atgaaagtta ctttaaaccg agctagcttt
    19741 atgcaggaat tgcaaactgt tcaacgagct atttcaagca aaaccacgat ccctattttg
    19801 acaggtgtaa aaatcacact gacacaagaa ggtttgactt tgacggggag caacgctgat
    19861 atatcaattg aaactttttt gtctgttgaa aacgaaaaag caaatatgca aatcgaatct
    19921 actggttcca ttgttttaca agcacgtttc tttagcgaaa tcattcggag acttcctgaa
    19981 gaaacattta ctttagaagt tttagaaaat aaacaagtag cgatcacttc tggaaaagcg
    20041 aattttatcg taaatggatt agatgcagat aactatcctc atcttcctgt tgtcgaaagc
    20101 cataaccaga tgaaattacc tgtacacgta ttgactaaac taatcaacga aacagttttt
    20161 gctgtctcac aacatgagag tcgtccaatc ttgacaggtg tgcatttcat tttatctgat
    20221 aattctttat tagctgtagc tactgattct caccgtctaa gtcaacgcgt gattccagta
    20281 gaacaagcgg ctgatcattt tgatattgtt attcctggaa aaagtttgat cgaattatct
    20341 cgctcattaa caaatgaaga agaaatcgtc gaaatcagca ttatggaaaa ccaagtgttg
    20401 ttcaaaacag aaacgatgta tttctattcc cgtttgctag aaggaaacta tccagatacc
    20461 aatcgtttga ttccttcaag ttttaataca gaggttgaat tttctgttcc tagcttttta
    20521 gcggcgatcg aacgggcttc tttactttct catgaaggcc gtaacaacat cgttcgttta
    20581 tctattcgtc cagatgcggt tgttttatac ggaaattcgc cagaaatcgg gaaagtagaa
    20641 gaaagcttga gttatacagc aagcagtggc gatccattag atatttcttt caatccggat
    20701 tatatgaaag cagcgttgcg tgcatttgga gatatgagca tcaaagtgaa atttatctct
    20761 gctattcgtc catttacatt ggaaccaacg gaagatggcg ttcaatttat tcagctgatt
    20821 actcctgtac gtacaaatta acaatgattt tcaatgttct tgaaaattta attgaaaaga
    20881 aatagaaaaa ataaaat
//
LOCUS       DAHUVV010000046        20740 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.046, whole genome shotgun sequence.
ACCESSION   DAHUVV010000046 DAHUVV010000000
VERSION     DAHUVV010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.046"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..400)
                     /locus_tag="NK767_001627"
     CDS             complement(<1..400)
                     /locus_tag="NK767_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694625.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLN"
     gene            complement(393..794)
                     /locus_tag="NK767_001628"
     CDS             complement(393..794)
                     /locus_tag="NK767_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694626.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            complement(784..1137)
                     /locus_tag="NK767_001629"
     CDS             complement(784..1137)
                     /locus_tag="NK767_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCH6694627.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            complement(1127..1438)
                     /locus_tag="NK767_001630"
     CDS             complement(1127..1438)
                     /locus_tag="NK767_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694628.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            complement(1435..2262)
                     /locus_tag="NK767_001631"
     CDS             complement(1435..2262)
                     /locus_tag="NK767_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694629.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLSFVNKKKCSYAYVKAIKAVVR"
     gene            complement(2272..3432)
                     /locus_tag="NK767_001632"
     CDS             complement(2272..3432)
                     /locus_tag="NK767_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCH6694630.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(3432..4118)
                     /locus_tag="NK767_001633"
     CDS             complement(3432..4118)
                     /locus_tag="NK767_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCH6694631.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            complement(4081..5259)
                     /locus_tag="NK767_001634"
     CDS             complement(4081..5259)
                     /locus_tag="NK767_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCH6694632.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            complement(5279..6975)
                     /locus_tag="NK767_001635"
                     /pseudo
     CDS             complement(5279..6975)
                     /locus_tag="NK767_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(6953..7267)
                     /locus_tag="NK767_001636"
     CDS             complement(6953..7267)
                     /locus_tag="NK767_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCH6694633.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            complement(7370..7651)
                     /locus_tag="NK767_001637"
     CDS             complement(7370..7651)
                     /locus_tag="NK767_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694634.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            complement(7656..8000)
                     /locus_tag="NK767_001638"
     CDS             complement(7656..8000)
                     /locus_tag="NK767_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCH6694635.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            complement(8026..8193)
                     /locus_tag="NK767_001639"
     CDS             complement(8026..8193)
                     /locus_tag="NK767_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694636.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEA
                     IPLYGKRIAKH"
     gene            complement(9187..9654)
                     /locus_tag="NK767_001640"
     CDS             complement(9187..9654)
                     /locus_tag="NK767_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HCH6694637.1"
                     /translation="MVLFDVKKYETPEAKDVDMDRTKHNVGVFLSAYLSARCRIGQPR
                     EPKVTASYSLVPSSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKLESNKIIIQFASSLELVAFK"
     gene            complement(10246..10533)
                     /locus_tag="NK767_001641"
     CDS             complement(10246..10533)
                     /locus_tag="NK767_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694638.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            complement(10530..10733)
                     /locus_tag="NK767_001642"
     CDS             complement(10530..10733)
                     /locus_tag="NK767_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HCH6694639.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            complement(10663..10932)
                     /locus_tag="NK767_001643"
     CDS             complement(10663..10932)
                     /locus_tag="NK767_001643"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694640.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            complement(10929..11459)
                     /locus_tag="NK767_001644"
     CDS             complement(10929..11459)
                     /locus_tag="NK767_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCH6694641.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            complement(11452..11766)
                     /locus_tag="NK767_001645"
     CDS             complement(11452..11766)
                     /locus_tag="NK767_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HCH6694642.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            complement(11766..12065)
                     /locus_tag="NK767_001646"
     CDS             complement(11766..12065)
                     /locus_tag="NK767_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694643.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            complement(12068..12229)
                     /locus_tag="NK767_001647"
     CDS             complement(12068..12229)
                     /locus_tag="NK767_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCH6694644.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            complement(12226..12543)
                     /locus_tag="NK767_001648"
     CDS             complement(12226..12543)
                     /locus_tag="NK767_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HCH6694645.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            complement(12790..15102)
                     /locus_tag="NK767_001649"
     CDS             complement(12790..15102)
                     /locus_tag="NK767_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCH6694646.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            complement(15117..15644)
                     /locus_tag="NK767_001650"
     CDS             complement(15117..15644)
                     /locus_tag="NK767_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HCH6694647.1"
                     /translation="MKIKRRMINMTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATK
                     SGAEFIDIDLIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSY
                     NGFDELLADFVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGP
                     ERSRATTVRNDNLPF"
     gene            complement(15619..16092)
                     /locus_tag="NK767_001651"
     CDS             complement(15619..16092)
                     /locus_tag="NK767_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCH6694648.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            complement(16083..17432)
                     /locus_tag="NK767_001652"
     CDS             complement(16083..17432)
                     /locus_tag="NK767_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCH6694649.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            complement(17407..18093)
                     /locus_tag="NK767_001653"
     CDS             complement(17407..18093)
                     /locus_tag="NK767_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCH6694650.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            complement(18212..18529)
                     /locus_tag="NK767_001654"
     CDS             complement(18212..18529)
                     /locus_tag="NK767_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HCH6694651.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            complement(18535..18966)
                     /locus_tag="NK767_001655"
     CDS             complement(18535..18966)
                     /locus_tag="NK767_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694652.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            complement(19223..19399)
                     /locus_tag="NK767_001656"
     CDS             complement(19223..19399)
                     /locus_tag="NK767_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694653.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            complement(19416..19895)
                     /locus_tag="NK767_001657"
     CDS             complement(19416..19895)
                     /locus_tag="NK767_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HCH6694654.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            complement(19828..20145)
                     /locus_tag="NK767_001658"
     CDS             complement(19828..20145)
                     /locus_tag="NK767_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694655.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            20313..>20740
                     /locus_tag="NK767_001659"
     CDS             20313..>20740
                     /locus_tag="NK767_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694656.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQK"
BASE COUNT         5641 a         4458 c         3232 g         7409 t
ORIGIN      
        1 tatttaattt atcgaattga cgaatcagaa gttctgtaat tggatcaagt gcaagaccta
       61 atcctcttct cataaattcg tccggctgat ttcttttaga agtacctatc cccaaatcag
      121 gatattttaa atactcaaat tttcttgtag gtctaatgat gaaacccaaa ttaatgtatt
      181 gagtcttaag tggacgacta ttttttgcgt gttggtgccc tcttcttaaa tctgcttcag
      241 aaacaggaat tttttctgta atcctatcca ctgcaatagc cgaacctttt gatttcaatg
      301 cttcgttaat cagtcgttcg ctctcgcttg aatagcgttc catccgcaca agaagttcat
      361 catgtccatt tatttttagc tcccaactat ttttagccat gacaatcact cttcaataat
      421 cgtctaaacg taaataccaa ttgatcgata tagcgatctt ggttctctag ttttaaatga
      481 ttgggatcca ttctctgaaa acgaatcgaa cgattttgaa tcaatgaaat aatatctagt
      541 gaatctcctg ttaaatcttc tctattttct gaatagaaag ttagatatag attttgaccc
      601 acgctatatt ttggctcagt gatcatttct atttctcctg tttcgagaat gaagtaatta
      661 aaatcatcag gtagctcatc ctcgcctacg gagtcttgaa agagtttgag gccaaaatgc
      721 tcttctaagg aagttttgat agcagaaatt tgcttattta aacgttcttt ttctttagaa
      781 ttatcaatca ccatattcac ccacactttc aagataaaaa tagatataaa aattatcgta
      841 atcggcatag ataacgttgt aacgcatact atcgattacg ataaaatatt gatctttatt
      901 aaatttcttg gcgattggat gaaatggagt ctttactttc ttagttaatt tcgatcccat
      961 cgcatccata gctgttatat cactatctct catggaaaga tttctaaatt ttaaagaagt
     1021 gatttctgta tcttctacac caatcttttt tcctagttca tttcttttgg tagtttgcgt
     1081 caaaatcttt aaccaaccat cgttgaatgt ttcttcgagt ctacgattat tcgccattca
     1141 catcacctgc aatatattct tgtagcgcat aatgttgaat gaaacctaat aactcactag
     1201 cgaaattttg ttcaaactca tctaaagcac gattccagtc gtatctacat ctttcgatta
     1261 gcaatccgta ttctaagctt tcaggagaaa aagaaagtgt tgtactcact ttactttgaa
     1321 gataaacagc atttttagct atcatctttt taattgactc atcttcttcg ttccaggtaa
     1381 cgtaaatatt atccttcaca gctattagca attcttcagt cacttgttca ggcgtcatct
     1441 aaccaccgcc ttaattgctt taacatatgc gtaagagcat tttttcttgt ttacaaatga
     1501 taaatcttca tcgtttgttg ttactccagc attgtgtaag atttttactt ctttaacttt
     1561 ttctattgga tcagtagcaa aacaaaagtc taattcctcg tgaactttag gaccaatatt
     1621 gaaatacatc atgttccaaa gctgtgccca catctcggct gtccagattt gtatatttgt
     1681 tttttgccct ctaaggtagc gatatagccg attagaatcc agataaacct ttttccaata
     1741 attcgcttta gggcggttaa taacccactg tgcgcctcct gaattagtgt ttatagtttc
     1801 caaagattct actgtaacat ttacaatgtt tgccatatct tttagaatat tttctccgtt
     1861 ttcacagctt ctaatataat caagacttag ataactacag cagtcgctac aataccaaac
     1921 atcatcttta gaaggcaatt tgcgcaaatt aattctttta ttgaaaatga catccgaatc
     1981 gatatagaaa tatcggtcgt cctcacgcga atgatcttct tctaaatatt tccaccataa
     2041 atatggttta atcgaaggaa tatactcttt gtcgtcccgc agatcatcgt acacatgaac
     2101 ttcaacacca tattccttct caaaaaaaat aggaatctga tcatcgtgtc tgctgaaaag
     2161 caatacgata tctttgattc ctagtttctt cagattagtt aaacaaactt caagctccca
     2221 tttaaaccga ttgattgccg gctgacaaag aatatacttc attctgatca cctacgcttg
     2281 tgttgtagtt gttgtggttg ttggttttgt agttgtagta gtagttccca aagcgctaat
     2341 atctaataca atgaaactat cgttacgttt aggttgaccg tttgcatatt gtttagctag
     2401 ataaatgcgt tcgtcttcaa caaaatggta ttcatctgaa gcttcaattt ttagtgtaga
     2461 tcctacaccc atgaagtaat ctgaggctac cccaataact gcttttcctt ctggcacagc
     2521 cgttgactgc aaatctgaaa ctggtactgg caatacttgt acgtattctc cattagcagt
     2581 tagtacagtc ttagctggga atactttaga ccagtaatca gttggattca caattaggac
     2641 cacatcagaa ggattcacat tacgataaat cggatcattc acaccttcga tattgaattt
     2701 tgatagtcgc gccatcaaac cgcccatagt tacagcatct aaagctgtaa taggttctgc
     2761 ttttttttca gcatattctc cgctagtttg tttgctcatg tcacgcatca ttccgactgg
     2821 catatcttta ccagtaccat caacaattgc ttgttctaat gcaattctca atgattctac
     2881 taaaacagta cggacataac gatctaacca tactggacct aaatcaagca ttgccttaca
     2941 tacaggaata taacctgata gcttgaactg cttcatgtta attacatcaa agccattatc
     3001 taaaactttt ttaacagctt cgcaaagttt accccaccat gctggattga ctccacgtga
     3061 cacaatccat tctgttacac cagttgtgtt aacaaaagta attttttgca atagtggatg
     3121 agattgttct aaatcttcaa atacacgttc aaatacagta gctggcacta attcttcgac
     3181 ccctgcaaaa ccttcgtttt tcactacttc gttatagaat tttgtttctt gtgtagttaa
     3241 tacacgctga ccacggttca ttaatactaa ttgatcttga ttttttgctg ttgcttcttc
     3301 taaaatttta tcctgaattt ccttagataa gcttaccata gctgcgctaa aagattcttc
     3361 gttaccatct ttaaaagctt tcatcaattg gtcgcttgca gctgttacac cttttaaatt
     3421 tttaactgtc attattttac atctccttgt ccaaatgttt tatttaatgc tgctgtaaat
     3481 gcagcaattt tttctgctct tttttcttta acgtcattca aaatttcttc aacgctttgt
     3541 tcttttttag cttcagtacc tgagctattt tctgcatcga taatttcatc gaccaatcca
     3601 taactcaaag ctgtttctgc atccataaac gattcttttt caagaagttc ttgcaatgct
     3661 tcatctgtgc cattgaatcg tgttttatat gaagccttta ccgatttatc aattgattcc
     3721 agttggtcag caatcgtacg gaagtcatcg acatttcctt ctccgtatgt ggaagcgcgg
     3781 tgaatcatca attgtgcatt gttgtagatt tttatagtat cgccagccat tgcgataatt
     3841 gaagcagcac tagcggctaa gccgttaatc acaacgttaa cttttgcttt atttgactta
     3901 agtaagttcc caatagcaat cccttgaaat acgtctccac cgtttgaatt aattactact
     3961 tcaatttctt cttgatcacc tagactatcc aaaatatttt tgattccctt gtcagtattc
     4021 ccttcaaaga accaactaga accaataaat ccctgaataa aaatttgcgg tactgcgcct
     4081 tcattcttta ctgctagaaa tgttttcatt gtcgtcattc gcctcacctc ctttcgatac
     4141 ttgttgattg tttttagtta taaatatttc atctgccatc gccttatcag agcgatcatt
     4201 tccaacgcgc tctcttcctt cgttgattgt aaatactcca tttctaatgc ctacatcaat
     4261 agcgtcaacc aaatctttga agctagtaat cttgatcata gttgtatcca cacgtacaaa
     4321 attccctgac aagtattctt ctacttcata gagactagcg ttaaacgcat cctgaataag
     4381 ttcagcaatc ggtatgattt cgaacattaa aaaagcgtcc acttgatccg ataacccact
     4441 catgtctccc tttagtaggt ttttcggaac gtgaaacgct gctgctgtca tctcaaagat
     4501 gtcgtctatt aagtttttta tatctcttga attgctttgg aagtttccgc tgaaatcttc
     4561 taatgtgtac tcattttgta attgaaatac cgcacctgca ttatcagctt ccataaaagc
     4621 cttaaattgt gatgtcatca ttttattgat ttgatcttgt gttgtattgt cttgcggtcg
     4681 gaataaattc cctttcagta cgtatctacg agcgttagag cgcttgtaaa cattcatggc
     4741 actagaaatg agtttcccat acgcttgata atacgcatcg actagttgcc taatttgttg
     4801 atctgcgtat tttatataga taacatcact ttctagaaat tctctatcaa ggactatgtt
     4861 gttaatttgc acttgagaaa acacatcatc tttcaatgca tattctgtga catcccaact
     4921 atccgcaata aatatttcgc tagaattatt agacggagaa acgatcaata cttcattgta
     4981 gaatattaat ctcctgatca gtttttttct aaattctgtt gcattatttt tcttattagg
     5041 agctacattc agcctatagt aaagatcatt ctttttattt tttccatctt catatgactt
     5101 gaattccgct ttactcatcg catttgcaat caaatcaata caagtttcaa tcgcaaattt
     5161 tcgatacaca aaatcaactt gcaatttaca aaagtattct tctaaaggaa ccgttgcttt
     5221 ttttgtgaag tatcctaccg ccttttgaaa aatccccact ttctcacctc ctttcaagtt
     5281 agaatactag aggagtaaat ccagttcctg tattttctac tgagctattt gtgactgtta
     5341 caggagcaga atcataaata tcatctaaaa aattcaaacc atgaaggaat gaaaaaaagc
     5401 catccgtttt tctagtttca ggttctattt tttcatagcg tatatttcca ttagaaatat
     5461 gctcttcata tacattcatg caataccaac gcataatcgc atcgtcacca aaaaataaac
     5521 gttgattaat aaaaaggtca tcaaccagat cttttaacat accatgtgta acagatccgc
     5581 ttcgaacaat ttccacagta aaacctgctt cttctaaagc gggcttcaat atttttgcac
     5641 ggtacatatc catagcgatt tttttaatat aatatttatt actcatttca agaaaccaac
     5701 ctacaatata atcagcttct atattttttc catgaacgat ctgtgatttt ccttgatcta
     5761 tagaaaaata tctataacct ctcgtttgat gttttgtaat cgaagggctg attcgtggat
     5821 aaaagtatgt tgtgtaaaat aaacatcttt atcgtatttt cctagcaacc caacgctggc
     5881 aaaatctcgt ctatcagcaa aatcgactgt tcctatcact tcatccattt tttcaggaaa
     5941 ttctttttct ttcgtatgca gaacatcatc atatgaagca acagcaaatc gtgtatcttc
     6001 cataggtctg ttcattcgtt tggtcatgaa cgtaagtctt aaaccagcat tacgttgcat
     6061 ttgagagtat tcttgaaaca ttttccgttt taaatctgca ttgtaattaa tagttggaca
     6121 agctttttcc cacatgtcgg gatcatcaac ttcattatcg ttatccaagc gacaaataaa
     6181 tggaaacaaa ctagaaaatt ctgctccatc cttgtcaatt ccaagttctc cagaaagaat
     6241 catttttgat tcttctataa tgtcatcaag cggaccacca cgaacatgac cattagttgt
     6301 atcataaaat tctctataat ctcgaatttt accaccacca gaagtagcca catttatcat
     6361 tgaataatct tcattttcgt gaatttcatc aaagcggttt gcacctggtc gcttcccatc
     6421 ttttgttcta gcatttgccg tgttataacg aagtttgctg tttgtagcga tattttgaat
     6481 aacttccttc gtagctttaa atactttttt atctaaatca ggatgatctt taattacttt
     6541 aaatacatca tcaaaactag tctttgcttg gctttcatta ttggcataga tatcaatatc
     6601 ataattttta ataccgtgtt tagcggttag tagaaagaag ttgttccaag aagcaaaacc
     6661 agttttacca ttaccacgtc ccattaatga aagatatcta ttgaacacta gtgttttatc
     6721 ttttttccat cgaacaccat aaataaaaca ttgtagaaat ttttcccacg gaattaattc
     6781 gaatggaaag tattgtgctg gtatattgat tgaatcctct accatctgct tatcgaagta
     6841 aatatcttct ctagtaaaga ctctttcttc tagataattt tttagcaata attgctcttt
     6901 gcataccttg atagtgcctt cttctatagc tttgaaccaa ttttcaatat gcttataact
     6961 caggaattga ttcatttgct tcacctacca attcaggagt aatggcaagt ttatccaaca
     7021 tcaatcccat ttgtttgttg acagaaacaa gcaacgctac tgattcattc tttttaccat
     7081 tctccagtct aatgccgttc tcggatatat cttcttccag tgatatcgcc gtttcccata
     7141 aactgatata acgatcaaca ttatctaaga atggctcaat atttgttttc tgactttcca
     7201 attggcttat taaagagcgg cgtaattttt ctctgtagcg attttgagac aattcgtttt
     7261 taaacatttt agccctcctt tcatgataaa gttcgaaaaa atctcttttc ctgacagccc
     7321 cctccgtttc atcaccccca aaaaatttgc gatttatttt aagggggggt tatctcacca
     7381 tcggaatgaa agcttcagcg aagtcaatgt aataattaat ctcttcaatg ctatatccaa
     7441 aaacattttt tattctttca acgttattat ctttattcaa cgcttctctt acttgattca
     7501 ctttgtattt actacaacag ttatctgata acagatccct aatacctaca tagcgaacgt
     7561 atatcaaacg tttaatcaat ccttgtgtat aagatgaata ctcttcaatc ttctctgtgt
     7621 catactctct gccattgtca ttgatgatca tgcacttacc acctttcact tgcatcgaag
     7681 ttagcgaagc tttctatctt cttctcttgt ttatccaatg ctgtaagata tctgccatga
     7741 acttcattat gatgttcaac acataaacaa ataagattat ctaaatctaa agctaagtca
     7801 ggtctatcct tgacttcctt tatatgatga acgttctcta ctctatgata cttacctagt
     7861 cttctacact cttggcattc atagtgatct cgtttcatcg ctttctccct aagcctgcgc
     7921 catttaggag actgatagaa cttaaccaaa cgatcttctc ttatcaactg taatagccat
     7981 ctatagaatt cctcagtcat gacccgtctc ctttcgcaat cttatttaat gcttagctat
     8041 tcttttgcca tacaacggaa tagcttcatt gttttccttt cgtttatatg tatcactctt
     8101 tattggtctt ctatactttc gtactatctc accgttacca ttttgcacag tgattacttc
     8161 atacttctgt tctaagtatt gtggtctata cattgttgtt acctcctttg tgcaaaaata
     8221 aaaagaccac tcaacgagtg atctcatatg taatgcacag gcagggacgt ttccgatcct
     8281 gtgcttgagt catttgacga ttcatttgta ccaaatccca aaactcaatc taacctaacc
     8341 aactatgtaa tagcaaccta caccgattcc atcgattact atcgacctcg ccttgctcgt
     8401 gtactttgag cgcccatttc caaccctcag ttgctaatga atactgaaac agaataccgt
     8461 ctctgttcat gaccaacgcg acagtacaaa gctttgtcgc aagcaggtta tcgtattcct
     8521 aataacccca actaatattt ctctcctaac ctacacctga gagtggcgca cgtctgcgct
     8581 agtattttat gccttttaac tgctagtggc atcccgacta gtcagccaac caatcggcat
     8641 ccgataagat tggttgtttc cgtaggttcc ttaagtcact ggcaatgaat cgaacattgc
     8701 atggttgccg aagcattgac ctagcacgca tgcttagcgt ctaccctttc cgccacagtg
     8761 acactataaa attattcttg gctgctacta ttttttattt tgcccatttt taaatccaat
     8821 catatagaca ttaagacaga gcgcaaaaat tgaaattatt aatggaatca tttctcttca
     8881 cttaccttta gttatcgtgt gaataattaa aaaaacaata gacagcaacg gatgatagat
     8941 aataagaata atttagaagg agttgaaatt cacatcctta ttcttaatat ttccgctgct
     9001 gtctatcgaa gcttaattaa acgatgaggg agatttcctc ccttacattt tattttgtct
     9061 cagacctatc actaatcttt cgacactatc ataatacaac attgaatagg taagtgattg
     9121 gtataaaaag ggtataaaat ggaaaccaaa tgggtaataa aagggtataa aaaatgtaaa
     9181 aactggctac ttgaaagcaa ccagttctaa cgatgaagca aattgaatga taatcttgtt
     9241 tgattctagc ttgacagatt cttcactcgt gttatttctt tgagcagtta cataaatggg
     9301 cagaccattg atataacgat catagaatat cttcttgcgc ctttcagtca catcaggctt
     9361 atgcggatgc tgtatcgctg aatagcctcg aacaaacaat ttatgcagat actcaaattc
     9421 ttcttgtgct tcttctttat cgatcagcat tctttctgct tcaaatatat gatcagctgt
     9481 agaagatgga accaaggaat aagatgctgt cacttttggt tctcgaggtt gacctatcct
     9541 acatctagct gacagatatg ctgaaagaaa aacaccaaca ttatgttttg tgcgatccat
     9601 atccacatct tttgcctctg gtgtctcata cttctttaca tcgaaaagta ccatccattg
     9661 attcctccat ttatgatata ataattgtgt cagaattatt aattaaggtc ggaggaatcc
     9721 ggcttttttt atacccatgc ttatgctaag cttttcaagt agcgaggttg cactcgttac
     9781 ccatatacat gttgagctat ctggcggaaa acagatggct cactatttca atattctgct
     9841 aaggacagcc agtggtcggc tgtctttttt atattttaat gagaagcctt acttattttt
     9901 ttatctttat tacgaaaaat gatattatct attgataata aagcgtatag gagatggttg
     9961 agtgactatg tggagcatgc tgttattttg gattcccgtt tgtattggta tcgtcgcatt
    10021 ttgttacttt gtcaaacact ctagaacaaa taagctcctc atgttatctt ttttacctat
    10081 agtatttttt attgtacaaa ttgttaaata tacctatatt gaatcgcaag aaatattcat
    10141 tttttatgtg gtaggtttat ttatctctgt ggtctttttc ataatgatac tttcctattt
    10201 ttataaaaaa taaatttttc tcttagaagt acattttttc gctgtttata taacctccat
    10261 atccaccaat ctcgctactg ctaaattctc tttgcttttc gctaaccact tgtcacattc
    10321 catcgtgttt tcaatacgaa tgattgctga gtgattatag agatgttcta catatccaca
    10381 aaatggatag atgaatcctt ctgcttcaca gcgaaccatg tcaccgactt tgaattttgg
    10441 tttctcacgt gttttagggt tctttgtcgg catatctagc attaaaccgc cgataccatg
    10501 actgctggag taaaatccgt cttttagttt cattcttttt cctcccattt acgatcatca
    10561 cttaatatcg aaattccaaa cttacgaata gcgccgctcg catcagcaac acactgactt
    10621 gccactttat atgtttcttc tgctgaaatt ccatattctt tttcaaactt tgtctttagt
    10681 acattcagtt cccgttttct tagttttgtt attctgcggt gtctgttgtt cattttgttt
    10741 aactttcttc tctaatccat ctcggacact acgagcaaaa atttctatgt tttgctgcgc
    10801 cttttctctt gtaatagctc ctctgataac caacctaacc agttctataa gtttatcttt
    10861 agttagttta tttttattct tctcaaaatc ttcggacaac ttttctaatt tagtaatgta
    10921 tgggtcgctc atccttctac cacctcatcc actggcacag caaacggcca gtatctttca
    10981 tcaattgctt tgatttgttt ttcagttaat tgaaattttt gagtaacgta ctgtgaaaat
    11041 tcatctgctg gaactaatat gacaacgttt gtattgctga atactttgac aagatatcta
    11101 ccattgatta ttacgcaata caacggctct ttctcgagct cgtagccaaa cagccatgca
    11161 cgcatataaa tttcaggatg atcaactagc caatcaattg cttcttcatg ctcaccgtct
    11221 ttagttttgc gataatacca atcatggaaa gttatcaata aagctaataa atcaacatct
    11281 gttgactgac gataatagtc gagtagttcc gccacaaact taggcacaac tggcttctgc
    11341 ggttcatacg atgggaaacg tttaatcgtt tctaaagcgt cactattaga tcccacacca
    11401 ggtattttca ttaattcatc aatcaatgct tgtttatcaa ttaatacttt tttattcatc
    11461 gctgtcctcc aaatcactcg acttcacgaa tacaccatct accatttttc ctgtgcgtcc
    11521 tttgatttcg ttgtatgctt ggttcagaca ctcgtacaaa tccatatcat tttgcatagc
    11581 taaaataatc aaggttacta ctacgtctcc aattccgtct cttaaaccat attcatcttt
    11641 tctagctaga gaagcggcaa cttccccaat ctcttcgatc gtttttaaca tttgcttgct
    11701 ggaatcagct tgatccaatc ccttatcttt tgcccactgc tctacttttg tgattagttc
    11761 ctccattatt tcttctccca atgtttaaat tgacgacctt tcgaatcaac gttctcgcgt
    11821 ttagctctat cccagataat gtttttgctt aatccagtaa tttcagataa ctgttcagca
    11881 gtacctgtta ctagaattcg gtcaccatgc cagattgcaa tttttctcgg tgttctccgt
    11941 ttggtttttt cactccacat tgatttaccg agcttttgga cttctgcaac tatttctttg
    12001 tcttcttgcc aattctcaga atgtgtcagt tcgatgattc gtttcattgc tgctttctta
    12061 tccacgctca ttcctccaat cgatggattt cccttcttaa attctctatg tgcaaatcga
    12121 ttgccttcct cgccgtttca ttgaccatca ctgcctttgt ccgttccaga tcgtcaatct
    12181 cacgctgaag gcttcgaata cgcatttgaa tcacttcttc tgttgtcatg atgattcctc
    12241 cacaattttc aatgcttctt ccactgatcg tgcaacaccg tatagtacag gttgaccgtt
    12301 taaaaattca gcaaaccttt tttgatcttc acgcaactta ccgcgttcat tttttacttc
    12361 tatataaatt gctttcccat cagaattacg atgtccacat agatctggga acccttttgg
    12421 catcccgtaa aattgccgcc cttcgatcgt tgtcacttgt cccgcattgg atcgaaaaat
    12481 cgtgcaacca tgttttgata aggcaattct gatctcgtct tgtatttgat gttctgattt
    12541 caatgtaacc ctccagactg tttaatcgtt ggtatcaaag catttaggaa gattttggtg
    12601 gagagtttgg agggtcagac ccaaaaacct attctttttt atataattta ctttttattt
    12661 tttactttaa tactttatta tttttttaaa accctccaga aagaaagaaa aatataatat
    12721 aaatactgat atatcaacgt ttctaagggt tggagggttt cgttctcaac cctccagaac
    12781 cctccagatt tatttaaaaa gttctctaac gttatcgtat tttcttggtt ttaatgtgat
    12841 tcctttataa accataaatc caccagcttt ttctttgcta tatttctttg aaatctctct
    12901 gccaaacttc gtgttagtga aagtatgctc gccattatct gatgcccatt cacgataaac
    12961 tttgtataat tctccgcctg atatttttag tcctggacct gtttcgcaac acgtttcgat
    13021 aaatgtacct atttgatcca tttcgtttcg ataatcctga ctcgctgcaa caaccgactt
    13081 tggtttctcc aagccctctc tctgccacaa caggcaacca tcgattgccc atttaaggat
    13141 cccttgcaac tccgtctgca atttaaattt cagattttta tcctttttgt gatccgggat
    13201 ttggacagtg aatggaatta aatttaaccg tcgccagatg ccgtcatcgg tcccacggat
    13261 gatgggcttg tggttcgttg ccagccacaa cttgaattca ggttcgaact cgaattcttt
    13321 tccataaagg tgccgagccg tgactttatc accaccagtc aattgtttga ccagaccttc
    13381 gtccagtcgg acgccttcat tcggttctga cgaagtgacc aaccgcgcac ctttcaatcg
    13441 tgcaatatca ctgtttgctg atgattggct ttgcttgacc atgatcgttt gtgcctgcat
    13501 agtcatcgca tagtttccca tcaattcaga aataatttcc aaaaataccg actttccatt
    13561 tgacccatga ccgtagagga tgaacataca ttgctctctg gtagatccgg tcagggaata
    13621 tccgacacat ttctggatat atctgattaa ttcttgatca ttgtcaaaaa tctgattaat
    13681 aaattcttcc cacataggac aatcgaccga ttctgtgtac tcgacatttg aaacacgtgt
    13741 aaacattttg tctatctcat gctcgtgtag aagaccgtca tgcaaagaca gatagccgct
    13801 ttgtgtgttg aacagcgttt tatctttgtc gaactcttcc ggcaatacgg atagtcgatg
    13861 tttcaattcg tccatcatag cgttttttcc ggcattgctg cgagaacgct tgacgtgttt
    13921 ttctttcgct ttcatcaaag cttctttggt ttcatcgtct gcgccttcag ggatcgttaa
    13981 cggttctttt cccatgatgt ctactgtcgt atcgatcatt ttccgaactt cgccggtatt
    14041 gtcctgtagc caaactttcc catcgtagta ataccaagat ttatcaatgt acgaatattt
    14101 gactaacgtt ccgtagatat cagtaaaccg atccgcgttg ccggtgtcgt cgtacgaata
    14161 gtatttttcc tcttttggct gttcctggtc tttgataaaa atcttaaaat cagatttccg
    14221 tttggggttg tagacggaat ttgtatcagc aattgcctta tttagtagag attctccgta
    14281 agttgccgat cccctttttt ggtcatattt gctacggatc attgcagacg ctctgaaaat
    14341 ctcatccatt ttttcaaagt ctctaccggt ccaaaaagcc aagtcgttgg cgaacgccat
    14401 atccgcttcg gattgcgagg aataaaaagc ttcccaaccg ccgttcataa agactttgaa
    14461 tcttgcccct tgggaagacg attcagcacg tttgataatt tcttcaaccg gtaaatcaac
    14521 ggtattcatc agcggatttt cttctttgaa ttggatgatt ttttcatctc caacgtagcg
    14581 atcatatagg cgtttgatat cctttggatt cggctcagtt atttctaaat attttgatgc
    14641 ggcatttcca gtcataacga agaatcggcc gttctggtac atttctacgt cacctttcct
    14701 gcgtcttccg ccgggcaatt ctccgcgagc aataatgtgg atgccagtac ctgattgaga
    14761 atactcagca taggatctca tgctttcgat aaattcatag ataatatttt cttctatatc
    14821 acctgattta aatcgctcaa cttcaccttc agcattatcg atatcaatgc cgaaataagg
    14881 tggtttgaaa aagaacccca gtccactcat tccgtagtga tcaatagcag cgagagcggt
    14941 ctgaaaatca gaccaggtac tctcgtcatt ggatttggct ttatgcccgt tgttggcgtt
    15001 ataagggatc ttcgtgtgct tctgccgctt ttcatcccaa actagcttat aaacacacca
    15061 ttgtttgaga tttttaagct cttttggaat tttttcatac attgattaca cctcaattaa
    15121 aatggaagat tgtcatttct aaccgttgtc gctcttgaac gttccggtcc gaaggatggt
    15181 tcatcaccat ttttgaattg gtgattcatt acgccccgtg tgttcgtttt atcccaagac
    15241 ttcacattga cgtttttata tccatttgac tcttcagttt tcacgcgtac tgaggcggtt
    15301 ttcaaaacaa agtccgccag caattcatca aacccgttat atgattttcc gtcttccaac
    15361 tgtagcgctt gtgcaattgc catgatcata ccttcgttat atttgccggt agctttggct
    15421 ttccagattt ttgcgaaaat gtgtttattt tggaacggtt gatccacatc gttacgaacg
    15481 atcaaatcga tatcgataaa ttctgcgcct gatttagttg cattttctac agctttgttt
    15541 acgaccactt cgtacgtacc gtctttgatt tcgttccctt gttgcgcttt tgaaaagtct
    15601 aagttaaatc ctgtcatgtt aatcattctc ctttttattt tcatattttt ctaagatagg
    15661 tttgaaatat ttttcttctg ctgcaagtcg tgcttggatt gcttcttctt tggtgttgaa
    15721 atccttttgt aagacatatt ctcctttgaa atataatcgt gctttccatt tatttcttcg
    15781 tttatcccaa ttaacgcctt ttgttccacc gacagacaaa gccttaacgt aagttccttc
    15841 aacatggtat ttttcattag ctttttttaa attaacgtag ccactctctt cgcctgtccc
    15901 gttaacaaca cctcttttta tattagatag agaagattca aaaacttcgt tatttttatt
    15961 tctagcgatt acaatttgat tgcctgaact atctcttttt ccagtatcac ctattattgt
    16021 tacgccattt attattgaac cttgaacatc ttctgctttt ttaccaaaaa taccgcccat
    16081 tcctacaacc accccttcct cttagcagcc atataagccc aaccaggttt aaatccttta
    16141 gctcttgcta tggcatataa gtcctctata ctttctgctt catcttccgt catgttgtaa
    16201 tatttattgt tctcaaaatt caaagtaatt ttcgtttctc cgactttcat taattcagcc
    16261 gattcatcga tttctaactc tgactttcgt tcttcaatcg gctgcaactc tccgcaatac
    16321 ggacaaatat tatcgccttt tggccgttcg taagcaccaa aacaaaacgc gcattgaacg
    16381 atagataaat cactatcagt ttttgttttc ttttttgtat ctaaactcca ttcacgatcc
    16441 atatcaggca atccaaaacg gttcacatta cccacatggt caatgatgat tgacgtttta
    16501 tctggtcggt aacgcatccc cctcatcgat tgctgaatgt acaatgataa cgattgagta
    16561 ggtctcaaca taattacagt cgaacaatca ggaacatcaa acccttctcc gatcaaatca
    16621 acattgcata ggattttaat ttcatgattt ctgaacgctt cgataatatc tgcacgttca
    16681 tcttttggag tttttgcatc gatatgtgtt gctttataac cagcatcgtt gaatatttca
    16741 gcagtgtgtt tacttgcatc gatactgtga cagtatgcta ttgcctgttc accttctgct
    16801 aacgttttat aatgtttcaa cacgtcgcca taaatagtat tcttcactgc cttatccatt
    16861 gatttctttg taaaatctcc agtcgaagct ttctttaatt cggctgtatc gatcaattta
    16921 ggtgcataat attcataagg agctaatcta tgattctcaa tcaaccattt agccgatact
    16981 ccttcaatta gcaagtcgtt cacatccccc aacccactac cgtttaaccg gatgggtgtc
    17041 gcggtaaaac ctaatcgcgg aacgtccgaa taatactcat agatttttct gtaggacgct
    17101 gctaacccgt gatggttttc gtcagtgatg atcagttgtg gtttcttgat ggttttaaga
    17161 tgtcgtacga ctgtttgaac catgccgaaa tttacaagct gattatctac gcccatcttt
    17221 tgaaacgtat ctttgatctg atcaatcaat tcacgacgat ggaccaagaa caacactctg
    17281 ttgccttttc gtgtggttcg tttagcgatc tctgcaacca tgactgattt tccagaaccg
    17341 cacggtgata cgatacatgg tgccttgtaa ccatcaacat aggcttgtct tgcacgatcg
    17401 actaaatcat tctggtagtc gtacaattcc atctaaaatc agctcacttt gcttacagcc
    17461 agatctatta tccaattgat ttttggcata ggttgaatct gtggcttgta aaataaatcc
    17521 gcgttcatcg tcttccttta ccatcaatcg accaacaacg tcacataatc ccaaaatatt
    17581 gtttcgaatc tttcgattga tctgcggcaa agcgatgttg taagaagatc catccgaatc
    17641 cgtccacaaa tcgatttctt cccatgcggt ccagataagg ttgctgtcta aatttttcat
    17701 atagcgtagg ctgttcacca ttctaaattg catatattga taatcgcctt gcgaaggaac
    17761 gccgttgttt tttccttctg cgcctaaact ggacagaata caccgttcca attcgctgac
    17821 attatcgaca accacgttgt cataaacacc tacataattg gtagttaaat cagccaacgt
    17881 gttgccccag tcgttccagg tatcttcgtt gtcgatatat acgatatcga catttttctc
    17941 acctctaagg acttttgaag tccgatcaat atcaaggatc agtgttttcc ctggaaaata
    18001 tttagccgtt gaagttttac cttttcctgg agcaccgtag atcaaatatg ttcccttggt
    18061 ttgttcgata tccgtcgcct tcttaatctg catcaaaatt ctcctttcta ttggttaaaa
    18121 aagtttttcg attgtcggta tgctgcattg ttgatccact atcttcttca actgatatag
    18181 tgattctttt aatcattttg tattcactct cctactctgg ttcgtgcgca ataatttcaa
    18241 caccgacaat ataatcacct agtctgtcga attcatcttc tacaagtgta ctgtcgcctt
    18301 tatcgttagt aagttccata gagaaccaat cttcatcaag gtcacggaaa ttaactgggt
    18361 tatcctcgaa attagcatca tatagatcat ctgaatcaat agctattaat gataaaccct
    18421 tagtgttacc gcccactttg taagtaacat atcctttgta accccaaatc ataaaagaaa
    18481 ctttaattgt gtgtttagcc catgaaatat ttgggtcata aatagtcaac tctttcattt
    18541 ccccaactcc cctacctttt gatccgtata ctgccgtaac tcgctcacgc gctgttctaa
    18601 ctgctccttg tcgttttgca ctgtggatag ttgttggcgc aagctatcgg cttcctgttg
    18661 ctttgtagcg atctcctgtt gcttttgttc gacttctcgt tgcttggctt caatttcctt
    18721 ctgcttgtcc gatttgattt gctcaatttc ggctttcagc tgctcctgtg tgcgagtgtt
    18781 gttggatagc tgtgattcaa gttctgacac acgttgcgat ttcgtttgac cgtattgtag
    18841 gactgtgttg aaatttgctt tgatcgtgtc caaatcttga aacgcattgc ttgctgcgta
    18901 gccgataacg ccgctaccta gtgctagtcc gatgattgct gttgtttttg ctagtttgtt
    18961 tttcaatgtt gtttctcctt tttttgcttt atagataatt tagttcatta aaaattcatt
    19021 cccacgatta aaacggaagc ctagaaattg caaacaatgt aaatgcaaga gctactgtta
    19081 tgctgtaaat tgcgtataat ttgtaagact cagggcgaag cttcttcatg atatatatac
    19141 aaaccggtat gcacgaccaa atgaatgtta cgcagactat tacttgtaat acaaccaccc
    19201 tagccactcc ttccgtgtgg ggttatttgt ctaaatacaa ttccgacaaa ccaagcaagt
    19261 cggcgataaa ataaagatgt tctttcgtca aattttgaat gtcatggaca gttgctggtt
    19321 tgccgtcaac ctttattcct ttagcttccg cgatttcgat agcagctttc aaagcgtctt
    19381 ttacgttttc gtgttccatt atttctcctc cttcatcatc ttatcctcaa accttttgtt
    19441 tggacgagtt cagcaccagg aatatctccg tgcttcagtt cctccttcaa ttgcttttta
    19501 tccaatttgg gaggcacagg ggtaaagaat ccttttggaa ttaagttctc attgattaca
    19561 ttgaccgata ctggattatt ttgaattcca atgttgaata attcaccttt aatcttcgtt
    19621 ttaccagtct tttccatttc ctcttgtaaa tagtgcttga tactcttagc attgttcaaa
    19681 agcgatgtct tacgttcctg cagacgttta atttcactat cgattaactc agctttccct
    19741 tctacttctt taactatttt tgctaagttt tctgccttat actcgatcgc ttcattaatc
    19801 gaatcgagag tatcgcgaag aatttcttca tccaattgtt ctgccagttc caggactttg
    19861 atatatgact cgctgagttg gtaaagagtt gccattcttt tgtgcctcct ttaataattt
    19921 tgcaatttgt tcaaaagcta ggattgcctc atctaaatcc aattccacag aatcatcaat
    19981 ttgttcgaaa gcaaggtttg tttcttcaat atcacttgct tgatagatac caatttttcc
    20041 attgtcataa agatcaaata ctaaaattcc tgacgcatct atattgcgca gtttgtattc
    20101 gtcttttaaa aagatgcggt ctagtgtatc cgttgcaatt agcatttacg gttcattcct
    20161 ttctgtggta aaatatagaa agatagttta tttccctgac acgatcatgc ttgccggcgt
    20221 tcgtgtcttt ttttattttt caaaattact aattcttgca ttatttcttc tctccttttt
    20281 gatataattt gagtaaaaag gtggtgaatt acttgataaa aattttgagc gattaccaag
    20341 tcgctattac tttaatcatt tctatttcag gatttttttt atccctctat aacttattta
    20401 aagataaaag aaaaattacg ctctcctatt ttttagttaa gcatgataga agtaatcgaa
    20461 tgattcttac tggcgtaatt gcaaacccat caaaaatgcc aaactcaatt atcgaatgta
    20521 cgtatcttta caacggtaag aaaattaatt gttcacatta tcacgccaat ggttttgata
    20581 tgggaagcat tcataaaagt agccttttat ctcctatacc gttggcacaa gcaatctctc
    20641 ctggctactc agtcgctttt tcagaagttt tagatatgaa agatatatta cctggtgaaa
    20701 aacttgtaat ggtcataaga acagctaatt atcaaaaaaa
//
LOCUS       DAHUVV010000047        19480 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.048, whole genome shotgun sequence.
ACCESSION   DAHUVV010000047 DAHUVV010000000
VERSION     DAHUVV010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.048"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(2..232)
                     /locus_tag="NK767_001660"
     CDS             complement(2..232)
                     /locus_tag="NK767_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694657.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            849..1511
                     /locus_tag="NK767_001661"
     CDS             849..1511
                     /locus_tag="NK767_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694658.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1590..4817
                     /gene="fss3"
                     /locus_tag="NK767_001662"
     CDS             1590..4817
                     /gene="fss3"
                     /locus_tag="NK767_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCH6694659.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            4931..5245
                     /locus_tag="NK767_001663"
     CDS             4931..5245
                     /locus_tag="NK767_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCH6694660.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5258..5632
                     /locus_tag="NK767_001664"
     CDS             5258..5632
                     /locus_tag="NK767_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCH6694661.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5642..5986
                     /locus_tag="NK767_001665"
     CDS             5642..5986
                     /locus_tag="NK767_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCH6694662.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6057..7409
                     /locus_tag="NK767_001666"
     CDS             6057..7409
                     /locus_tag="NK767_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCH6694663.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7469..8611
                     /locus_tag="NK767_001667"
     CDS             7469..8611
                     /locus_tag="NK767_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HCH6694664.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8636..8890
                     /locus_tag="NK767_001668"
     CDS             8636..8890
                     /locus_tag="NK767_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694665.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9146..10330
                     /locus_tag="NK767_001669"
     CDS             9146..10330
                     /locus_tag="NK767_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCH6694666.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10327..10464
                     /locus_tag="NK767_001670"
     CDS             10327..10464
                     /locus_tag="NK767_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCH6694667.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10469..10690
                     /locus_tag="NK767_001671"
     CDS             10469..10690
                     /locus_tag="NK767_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694668.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10703..11203
                     /locus_tag="NK767_001672"
     CDS             10703..11203
                     /locus_tag="NK767_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCH6694669.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11300..13147
                     /locus_tag="NK767_001673"
     CDS             11300..13147
                     /locus_tag="NK767_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HCH6694670.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13150..14046
                     /locus_tag="NK767_001674"
     CDS             13150..14046
                     /locus_tag="NK767_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCH6694671.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14096..14485
                     /locus_tag="NK767_001675"
     CDS             14096..14485
                     /locus_tag="NK767_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCH6694672.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14472..15974
                     /locus_tag="NK767_001676"
                     /pseudo
     CDS             14472..15974
                     /locus_tag="NK767_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            16719..18545
                     /gene="ltrA"
                     /locus_tag="NK767_001677"
     CDS             16719..18545
                     /gene="ltrA"
                     /locus_tag="NK767_001677"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCH6694673.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18679..>19480
                     /locus_tag="NK767_001678"
     CDS             18679..>19480
                     /locus_tag="NK767_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCH6694674.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         6575 a         3186 c         4279 g         5440 t
ORIGIN      
        1 attactccga tctattttta aacttagttt ctacctctaa tatatatcga gtttttattt
       61 tgccttcaga taatactgtt tcttttttcg caattacagg ttggttattt tcggaaaaag
      121 ctaatatagc taagattgaa acatccatct ggaatttatc ttttttgctg ctttgctcat
      181 aaaagtctgc atattcatca ctgatatttt ttctaataaa gtcttccatc ataaaaatca
      241 cctcaaaacg attatacgaa cttacgttct cgttgtaaag cgatatttga ggggcaaaaa
      301 gggggcaaaa atacgtttac ctttgatata tctcagattg aacatagtgt ttattatatg
      361 tcggtattgt tgatttatag ctgttttgag ggtattagat acataataga atatagtggt
      421 tttgtttacg tgcattacac ggaaaagctg ctcgtatcaa agaaatcaga agataaacac
      481 ggaaaacttc cttggaaatc tgaaatttgt gaaaagtcct tgtatatcaa gggctttttc
      541 ttttttctct atcacagttt taagccattt ttggctgttt tggaccattt ttcaatcata
      601 ttcccttaaa aattgtccta gattgtccga cattgactat cagtgtcgtc aaaatagggc
      661 aaaatttagt agtcagcttg acgacaaata taatacgaaa aataccaaaa catttaccga
      721 atttagcaac ttttgaaccc aagtttctca aagggtcagc tattagcaaa aaagacaaaa
      781 catgtacttt tctactaaaa agttgatgtg ataggcttct atctaaataa ctattaggag
      841 tttttatcgt gaagcaaaat gatggacgta tcatttggaa aaatcaaagt gagttaaaac
      901 tgattttaac aattaatgaa ttcatcgaaa aacacggaat tacatctagc cgtcaatacc
      961 agaaaaagct atcagaaaat ccaaattctg caccaagtat gtggttcatt aacaaaaaat
     1021 atggatcatg ggagaattta ttgattagta taggacgaga aaatactggg tatggaaaat
     1081 gggctagaat gtcagaacaa gaattattgg aaatagtaga agcctttatt aagtgcgaga
     1141 agataacctc tcaacgaatg tacgaacaaa aatcagtagg gaagaatatt ccttcactta
     1201 gtaccattaa aaagatgtta ggagatatac gtccactttt taaagaaaaa aatgatggat
     1261 ctcgatttac tgatttcgaa ctgctgttag aactaaaaaa tgaaataatc cgattaaaac
     1321 tacaggatga tttatcaatg acaaagtttc gaaaactagt tcaatcccca aaactaccat
     1381 cagttgatac aattatgaaa agaacgaaca aaaattggga ggaattgatg gcagagattg
     1441 gatttgatta tcgaagaatc aaaatttata agcagagaaa caacctatcc aagacaaaga
     1501 aaactaaata gtatcactct aaatcagacg ttcaaaatta aaatttggac gtctttttta
     1561 ttacccaaaa acgaaaggag atttatttta tgaaaaagcc aagatttaag aattggcgtt
     1621 tgctcgcaac actgagtcta ttatgccaaa caattggtgg atcgcttggc ccaacgattg
     1681 cctttgctga tgaaattact catccacaaa cggtaactgt ggaattggat ttggctcacc
     1741 agtatgctgt cgaaggtaca tttagcgatg gtcgtcccat gtcagaagtt accgttccgc
     1801 actatgcagt ttacaatggt gtgaagcaag atattttctg tattgagcca ggggtgccga
     1861 tttacaatga gttcactcct ggttatgaga aaaatccatt gcctgatatg tcggagaaag
     1921 cgaaattagt ttccgtttta tggaaggaag ctggtacgga tgtggataca catattgtgg
     1981 cccagaagat gatttggcaa gaagttaatg gctacacgct tcactcaatt aaacgttctg
     2041 atggtagtgc agtaaatatc gcagccattg agtcaaaaat caatcaagcc attgcggatt
     2101 atcaaaagaa accaagcttc cataactcca cagtgaaaac cgtgttaggt caatcgacta
     2161 ctgtgacaga tacgaacggc ttgaatttat cggagtttga tgaagttgtc gagaatatcg
     2221 caaatattga ttatcgtgtg aacggcaatc aactagtgat tactccaaat gccaattcaa
     2281 aagagagtgg tgaattaacc ctaaagaagt cagctaatac aggaacaccg gtagcttata
     2341 aaatggctgg tcaacaaact ttgatggcgg gggcaattga taagcccaat acctacacaa
     2401 tcaaaattga tgtggaaact gagggttctt tgaagattaa aaaagtcgat aaagaaacag
     2461 gaaatgttgt accgggaacc gttttccatt tagactttgg aaaaacatta cctgcaaaag
     2521 acgtgaccac cgataaagaa ggcattgcga cattggatga gattcctcat ggcgcgaaag
     2581 taaccattac tgaaaaatca gtgccagctc cttatacgat tgacactaca ccaatcaccg
     2641 caaccattaa atctggtgag actatttctg tgacttcaaa aaatactcgc gaaaaaggtc
     2701 aaatcatttt gaataaaatg ggggtggaaa caggaactga tctttggaac gacaaatact
     2761 cattagccgg aaatacgttt gccattcgca aagacagtcc aaccggtgaa attgtccaag
     2821 aaatgaccac ggatgaaaaa ggtcatgcgg aaacaccaaa agagattgcc aatgcgttgg
     2881 aattgggaac ctattatgtg accgaaacca aagctagtaa tggctttgtg aataccttta
     2941 aaccagtaaa agtcgaattg aactatgtca atcaaaccgt ggctcttgtt accagtaacg
     3001 taaaagggca aaaccaagaa atcactggtg aaaccacttt gacaaaagaa gacaaagata
     3061 ccggtaatga aactcaaggt aaagctgagt ttaaaggagc tgaatatact ctctttactg
     3121 caaaagatgg tcaagcggtc aaatggagtg aggcttttaa accagagtta gtgaagggaa
     3181 cgaaagcttc tgatgaaacg gtgactttga ctttagatga aaagaatcaa gttgctgtta
     3241 aacacttagc gattaatgag tattactggc aagaaaccaa agcacctgaa ggatatacct
     3301 tggatgaaac aaagtaccct gtttccatca aaaaggtgga tgataacgaa aaaaatgccg
     3361 tgattacccg agatgttacg gcaaaagaac aagtcattcg ctttggcttt gatttcttta
     3421 aatttgctgg atcagctgct gggactgccg aaactggctt caatgatctg acctttaaag
     3481 tatcgccatt ggaaggaacc agtgagatta cagatgccga agatgaagca attacagctt
     3541 ataacgagca actaggtttt gatggctatg gcaagtttga aaatcttcct tatggggatt
     3601 atttacttga agaagtagag gctccagaag gatttcaaaa aattacgcca ttagaaatcc
     3661 gttctacttt taaagaaaat aaagcggatt acactaagag tgaatacatt tttacgatta
     3721 ccgaaaaagg acaaaaacaa ccaattaaaa cggtgaccgt tccttacgag aaactgacga
     3781 ataaagcatt ttctgttagc ttgaatcgtt tgatgctcta tgatttacct gaagaagaag
     3841 atagcttaac ttctcttggg acttggaaag acggaggcaa agaattgaca gaccttgatt
     3901 ctaccgagct agttgataaa ttgagctata acttgcatga aatcaaagaa gactggtatg
     3961 tggtagcgca agccattgat gtggaagcga caaaagctgc ccaagaaaaa gatgaaaaag
     4021 ccaaaccagt ggtaattgcc gaaacaaccg caacgttggc gaataaagag aaaacaggaa
     4081 cttggaaaat tttgcataaa ttaaccgcag aacaagtttt ggataaaacc atcgtcttat
     4141 tcaactatgt gtatgaaaac aagaaaccct atgaagcagg tgatgatcca gtagcgaaag
     4201 atgctagctt gaacaatcaa gcccaaactg tcaattgtac ggtggaacgc catgtttcca
     4261 tccaaacaaa agcccaccta gaagatggtt cgcaaacctt tactcatggg gatgtggtgg
     4321 atatgttcga tgatgtgtcg attacccatg atgtactgga tggctcaaaa gaagcttttg
     4381 aaacaattct gtatgcatta cttccggata atacgaccaa agaaatttgg aaatctggca
     4441 aaattgatta tgaagtgaat gacaaagaat tcactaaaac cgtacttgcg gaaaaagtgg
     4501 acacaagtaa atatccagaa ggtacaagtt ttaccttcaa agaaattaac tatggcaaag
     4561 acggaaacat caatgggaaa cacaatgagg atctaaaaga aaaaactcaa actttaacac
     4621 ctaaaaaagt tccgacaaca ccaagtgcac cagaacaacc ggaaatacca actgttccaa
     4681 gtgactctca agaatctagt cctacagtaa agacattccc acaaacaggg gagaaaaatt
     4741 ctaatgttct gctgtttatt gggtttacct tgatctttgc gactgctggg tattatttct
     4801 ggaatcgccg aaactaaggt gatgtgatga aacaactaaa taggaaaaac caacgtgcgc
     4861 gaccgcctcc taaaaaaagt ggtcgcgcta tttttgtatc caaaactaaa aatgaaaaga
     4921 ggaaaagaag atggaattga aatttgttgt gccagatatg gccgaaacct ttggaaagat
     4981 aaggtatgcc ggcgaaggcg aagttttaac tgaaggatat gggcggaata ctacagtaat
     5041 tggtcgtagt tatcatttgt attccagtaa gcaacgagcc gatgatattg aagtggtggt
     5101 agctgcggaa gccggcgaaa aggattttga tcaagatcaa ccactaaaag ccgtgaatcc
     5161 ccatttggtt gccaaaggct atgagattga gaatcgtggg tttaccgact acgtgttgta
     5221 tgtcgatgat ttagtgaaag cataggagga aaagaagatg agattaacag aaggtattgt
     5281 tgtagattca ggactaacgt ttgggaaatt acgattttcc gcattacgcc gagaagtacg
     5341 gaagcaaaat gaggatggaa cggttagtaa cgaggtaaaa gaacggactt ataatttgaa
     5401 atcctctgcg caaggtcgaa tgatccaagt tagtatccca gcaaatgtac ccttacgcga
     5461 gtttgcctat gacgcagagg tagagttggt caatccaatc atggatacgg tcgccaatta
     5521 tgttttccga gaaggaacta ctgtcaattg gttcattaag gccgatgatt tagtcttgaa
     5581 acgacaacca gatcagggaa attctacaaa ccaaaatgaa ggaaagaaat aggtgaagcg
     5641 tatggaaatt aatgttgaac atattttaga ttgtctcgat caatatggaa aaggtgagct
     5701 aacggaggaa caactcacta aagcattaac ctatgatgaa aaaatgtttc tgattatgca
     5761 tcaagggtta ctggaagtag gtaacgatgc cgaagaagat tttgatgtac tgaattggtt
     5821 aggggaagaa gagtccttct ttatggttat cgaagtaaat gaaagtctat gtcgagaagc
     5881 cgaaaccgta ttggaagaaa ttggtgtgga aatgccggat gccattgaag tttttttaaa
     5941 gcaactagtg gaaacgaaac aactccctat agcagtaaat gactagcata actgcttgaa
     6001 attggatcaa tgaaatccta tgaagctttt ttcgattgga ggtggaaacc ataaaaatgt
     6061 acaaagggca tcgtatacgg gcaggagatc agcacttggt ttatcacttt gttcttggct
     6121 ggctactcgc tctgttcatc ggttggatga gcgtctttta ttttcaagag cttagacaat
     6181 ttgatatttc taagctatca ctttctacta tagaaatcgt tcgttctata aaagatttaa
     6241 tctatttgct aggaagtctc gttctttcag gaagcacgat gttgctttat atacactttt
     6301 ttcaggatca ttggcgcagt ttgtggcatc gacaaaagct agcccggatg attctagaaa
     6361 atcattggta cgaagtaaaa caaactcaga gtgaaggatt tttcaaagac ctcaatagta
     6421 gtcgaaccaa agagacaatc agttacttcc ccaaaatcta ttaccgtatg aaggatggtt
     6481 tactttctat tcgcgttcaa atttcactgg gaaaatatca agatcagctt ttaaagctgg
     6541 aaaagaagtt agaaagtggt ctatattgtg aactagtaga aaaggaactc aaagactcct
     6601 atgtggagta cactttactg tatgatataa ttgccaatcg aattgggata gacgaagtag
     6661 tggcagaaag tggggctttg cgattgatga aaaatcaagt atgggcttat gattctttac
     6721 ctcatatgtt aatcgctggc ggcacaggtg gtgggaagac ttacttttta ctcactatca
     6781 tcgaggcttt attaaaatct gatgccgagc tttttgttct tgatcccaaa aatgcggact
     6841 tagccgattt aggtacggta atgcctcatg tctattccca aaaggaggaa atttctgctt
     6901 gtgtggaaga tttctatgag cgcatgatga ctcgtagcaa ggcaatgaaa gaaatgccca
     6961 actacaaaac aggagagaat tatgcgtttc ttggacttcc accaaacttt ttaatctttg
     7021 atgaatacgt ggtttatatg gaaatgttaa cgacgaagga aagtgcggtg attttgaata
     7081 agctcaaaca aatcgtgatg ttaggtcgtc aatctggttt ctttctgatt ctagcttgtc
     7141 aaagaccgga tgcgaaatat ttaggagacg ggattcgcga tcaatttaat ttctgggtgg
     7201 ctttgggtcg tatgagtgaa ctgggatatt ccatgatgtt tggcgaggtt gataaaaaat
     7261 tctttatgaa gcgtatcaaa ggtcgaggct atgtagatac gggagggagt gtgattagtg
     7321 agttttatac gccacttgta ccaaaaggat atgatttctt aagggaaatt agcgacattg
     7381 gtggattaaa cgttaatacg gaaggataaa ataatagtat gtagcctagg agtgtcaaga
     7441 atcctgggct ttttttacga ggtgatttat gacattttta gatttattcg ctgggattgg
     7501 tggcttttgc ttaggtatgg aacaagcggg tcaccagtgt atcggctttt gtgaaattga
     7561 tgattttgct cgccagagtt ataaggcaat ccatgataca agtaaggagg tggaaatgca
     7621 tgacatcaca agtgtatcag acgagtttat tcaatctctc ggaccagtgg atatcctttg
     7681 cggtggattt ccgtgccaag ctttttcaat tgcgggaaag cggcaaggat tttccgatac
     7741 tcgaggtact ttattctttg aaatcgctcg gttcgccgct attctccaac ctaagttttt
     7801 attccttgag aacgtccggg gattactcaa tcacgaagga ggggctacgt tcgagacgat
     7861 cctccgaacg ctggatggat tggggtatga tgtggaatgg caagtgctta actcaaaggc
     7921 ctacgttccc caaaaccgtg agcggatctt ccttatcgga cattctcgag acgcatgtac
     7981 cgaacaagta tttcctatca ttggatcgtc tccaacatct gatcaaaaca tcagaaactt
     8041 attaaatatc aacccttcca atcgaggaat ggggggacaa gtctatgggt cagatgacgt
     8101 agcgcctacc ttgaccggtg atgaaggaat caaaatcgca ttgccggtag atgatggaat
     8161 ttctgtagcg ggtatgttac tgggaaattt tgaacaaggc aatcgggttt atgagataac
     8221 tggaacagcg cctactttgt caacgaaaca aggcggaacg aagatcatgc ttcggaaaaa
     8281 cgaaacagaa tatcaagagg tgaagccagg gaacagtgtg aacctggctt ttcctaattc
     8341 cacaagtcgt cgaggccgtc tgggaaaaca aagtgtgcat accttattaa caggagacca
     8401 acaagcagtc gtcacggatc agtatcaaat tcgaaagtta acacctaggg agtgctggcg
     8461 gcttcagggc tttccggact gggcttttga tcgagcttct caagtcaatt cagatagtca
     8521 gttgtataag caggctggaa attctgtaac agttccggtc atttttgata ttgctagaag
     8581 attaaaggag gtaaaaacag atgatttatg atgagttgat tggggaatct actgggtgat
     8641 tggcaagatt cagtcagatc cggaattgga agaagaactg taccggctca attttgatat
     8701 tcgcaaaaac ggggtgaaag tccctgatga tccgtatgta attgatgagg aaacggatgc
     8761 tcgaatggag ctgaatcaag tcatcagtga attggaacga attgcggact ttgctaaaga
     8821 acctgatatc cgccaatact tgtttgagat aaaagcagag ttggaaattg agggtattac
     8881 cgcggagtaa cggccaaata aaccactgat aggcactgac cgcgggggat ttgcgaagca
     8941 gaaatcctcc gtggtgctgt gcctgtttat ggacggcgtt agccggccat gatgtctcac
     9001 cccccgtatc taacaggggg gtacaaaaac acaaaaaata agcaagcaac gataaggaat
     9061 ggctgtccta tcaaggataa taagcaattt gtgtagatgt taactgattt gaaaaagtta
     9121 acaccttaaa tttggaggtg agaacttggc acaaaggaat ttagattatc ggctattaaa
     9181 agaccggcgt aatgaatatg gcgtttcaca aaataaatta gctacgactt gtggacttag
     9241 tcgcccgtac ttaaatcaga tagaaaatgg tggcgtaacc gcatcgacaa aaaccatgag
     9301 gaaaattttt gaccaactgg aaagtttcaa tcccgagtta cctttaacgt tactgtttga
     9361 ctatgtaagg attcgctttc ccacaacgga tgcgaggaaa atcattcaag agattctcca
     9421 tttgaaattt gattatatgc tccacgaaga ttacgccttt tactcctatc aagaacaata
     9481 tgttatggga gatattgtgg tgatgttgtc acatgaagaa gataaaggcg tccttttaga
     9541 attaaagggt cgtggttgtc ggcagtttga aactttttta ctcgcccaaa agcgtagctg
     9601 gtatgacttt ttcgaagatt gtctaaaagc cggtggcgtg atgaaacgct tagacctggc
     9661 aatcaatgat cgagttggcc tgttagatat tcctgattta acgaaaaaat gtcagaagga
     9721 agaatgtatc tccttgtttc gtaccttcaa aagctatcgt tccggggaac tgttgaaacc
     9781 tgatgataaa gatggtatgg gcaatacttt gtatattggc agtcttaaga gtgaggtcta
     9841 tttctgtttg tacgaaaaag actatgagca atatatcaag ttagggattc ctttagacca
     9901 gaccgaaacg aaaaatcgtt ttgaaatccg actgaaaaat gatcgagctt atcatgcaat
     9961 tcaagatttg ttaaaaggtc gcagtattga aagtaccaca ttttccatta ttaaccggta
    10021 cttgcgattt gcggataagg tggaaggcaa aaggcgaact aactggccct tgaatgaaca
    10081 atgggggcgc ttcattggac ggaatcagaa ggaaattcaa ttaacttctg aaccgaagcc
    10141 ttataccatt gaacgaacgt tgaactggct tggacgacaa gtcgccccta cgtggaagat
    10201 ggccaaggag ttggatcggt tgaatcagac aacgtacatt caggatatgg taaataatac
    10261 tcgactatca gaccggcaca aaaagatttt ggagcaacaa agtatggcaa ttgaaaattt
    10321 gattatatga aaagaggaat cgtgatggaa attgtgaaag atattttatt ggtgtttggt
    10381 ggcagtatgc ttggtgttac tgtgatgtgc ttgatgcatg caagtgcagc ggcggaccga
    10441 gcaatggaaa agaaaggaaa gtgatgaaat gaatttcggt caaaatttgt atcaatggtt
    10501 tttaaccaat gctcaatctt tagtcttact agcgattgtg gtcattggtt tgtttttagg
    10561 atttaaacgc gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg
    10621 tctggtcttt aatgcttctg gtgtgaagga tgtcttactc aatttgttta accgaattgt
    10681 cggtgcctga gggggaatca caatggaaca gatacgtgtt tatattgcga accttggaaa
    10741 atacaatgaa ggtgagttgg tgggggcatg gtttacccca cccgtcgatt ttgatgaagt
    10801 gaaagaacgg attggattaa atgacgaata tgaagagtac gcaattcatg attatgaact
    10861 gccttttgag attgatgagt atacaccaat tgaggaaatt aatcggttgt gtggtttagc
    10921 ggaagagctt aaaggaacgc caattggtga ggtcgcttca gagattcaac atgccttctt
    10981 caattctttc gaggaaatgg tagagcatgt ggatgatatc atctattatc ccgattgtaa
    11041 tgacatgagt gatgtagcgt attacttgat tgtagaagct ggtgatttag gcgaagtacc
    11101 agaacacttg aagacgtata ttgattttga tgcgtgtggt cgagacttgg agattggcgg
    11161 gaattatttg gttacgagtc gaggggtttt tgaactaatg taaatcattt gttagaattg
    11221 accagaaatt tctatcgtac ttttactttt ctatttgata tactgaaata gaaaatattt
    11281 gttgataggg gtgttactaa tggttggcaa taagtggtac aaatgtgatc ttcatattca
    11341 ttcaactgcg agtcagtgct ttcaggataa aaaggttact gctaaggaat gggtagaaga
    11401 atgtttaaac aaaggactcg attgtgttgc attaactgat cacaatacag gggaaaatat
    11461 tgatgaatat aagaaagtgg ctatcgaaaa tggcttagat ttttttcctg gagtggaatt
    11521 aacgtgtgga gaaaatggaa ctcatttact agtcctattt gataaaagtg catgtacaaa
    11581 tgatatctct gatttcttaa ccaagatggg tataaaaaga gaggattggg ccagcgctgc
    11641 agcttgctca atgaaatcag ttttagaagt tattgatttt gctgagcacg agggcttgct
    11701 cgttattcct gcacatatag atgagtttaa tggaatttct tcagcaaaat ttggaaatct
    11761 aggaaagata tttgaatcag aaaatatata tgcagtacag acggttcaaa aagaattttt
    11821 tgaaaatcgc tcacaaatga ttccctcatc taagcgtgac actatctatg atagtgtgaa
    11881 tgccagatat gatggtagag ttggaaaaga tactttggaa tcttggtata aatcagttgt
    11941 tgaggcagaa aaaaataata tgacatttct tagcttttct gataacccac actcgaaagg
    12001 aaattcaaaa catggacttt ggggaatagg cacaagatat tcctatataa aaatgaagga
    12061 cgaacctgac ttgggatcac ttcgcgatgc tttaatgcta ggcgaaactc gagttcattc
    12121 tgatttttct aatttctcca ttaatgaaaa tgaagtttta ttggaaaaat taagcttttc
    12181 tgggaccact cttagcacaa aagaagttgt agtcgaattc agcgataatt taacttcaat
    12241 tattggagga agaggaacag gcaaatcttg tattacaaga ttcttggtct atgtccttgg
    12301 aaaggaagca gaacttgatc aattttctga aatacaaagt gattatcaga actttgcaca
    12361 aattgaacat aatggctctg gaatattttt gaaggataca attgtgaaac tgaatatttt
    12421 ctataagggg acaaaatatc aaataataag aacacaggat agacattcta tttatgaatt
    12481 tacacaagag aatggtctgg ttgaaaaaga aacagaacgg ttgagaatga tttccgataa
    12541 ggtgagtata tactctcaaa aacaaattta tgaaatttca aaaaatcaaa ctagtatact
    12601 agaattacta gatggatata actctgattt gatttcagag tataaaaatg agattgaaac
    12661 atgtgtaaac gaaataagaa aattaaattg ggatattgtt tctgtgaaaa aagaaataca
    12721 agataaggcg aaggtagagc ttgaaattga agatttaaaa aaacaagtag aaaagctatc
    12781 tcataagagt tataaagatg tttatgatga gtattcaaaa gagacagata tttataggga
    12841 actgaaacgt gatgtggagg cacttaagga aattcccaaa aatctaactg atagtgttga
    12901 caaaattgac tttggcaatg gtataaaagt tacagacgaa attgatgaac accgcggaga
    12961 attgattaag aatttagtga ccaatcgggc taagattcaa acaataatta atgaaatgtc
    13021 agatgaaatt gatagttata gagcagagct gaataaatca gattggaaag ttaactataa
    13081 tgaggtttca aagaggtatc aaagaggtat caaaaactta gtgaagagtt atctcaagat
    13141 gaattaaaaa tgttacaaaa ccttgattct ttatatcaat ctttatcaaa aactgaagag
    13201 cgattggaag aaattattca acaagaaata aagcttaaag attaccaagt tgaggttgac
    13261 tcagcaatgt caagaatgaa taaatcatat caaaatttct attctgagag aagtagttcc
    13321 tcgcgcgaaa ttttttctga tattacagga atagaggcta ctattcaacc aaatagggat
    13381 ttcaataatt atgttaaaga gctcagagct tgtattaata aggaggacac ttttgacagt
    13441 gagtttggat ctcttattga taagctgaca aaaaattcca ttactaatca agatatatat
    13501 aatgacatac ttgatataag agttagtggg gaaagtagta ttttttcaga taaaaaatta
    13561 atcaattcta taagaaaatt gaatgatgca caagttcttg aattacaaac ccttaagcca
    13621 aatgatttag tagttatccg acttgaacta aatggaaaaa aagttagttt atcgaatgct
    13681 agtgctggtc aaaagacatc tgcaattctt tctatgattt tggcgtatgg aaattcacca
    13741 ctcatattgg atcagcctga agatgatttg gatagtcaat taattaataa cttaatcgta
    13801 agaagtatca ttacaaaaaa ggaaaacaga caaattttaa ttgtaactca taatgcaaat
    13861 atcccagtta atggtgattc agaatggttg attagcatgg gagattcaaa agaaatatcg
    13921 gctgatcagt gcgggtcggt agatgaaccc aatatcaagc caagaatatg tactgtgatg
    13981 gaaggtggcg aggatgcttt tacaaataga gcgaaacgct atggttttaa acaaattgta
    14041 gaataacact agagggtgag acatattatt atgtccgccc tttttaggag gaaaaatgaa
    14101 aaaaataaaa agctatacca gtatctgggc agttgaaaaa gtcatttacg caatcaatga
    14161 ttttcagttg ccttttcctg tgacttttaa ccaaatggct tggttcgttt tatctttact
    14221 gtttgtcatc gtatttgccc atgtgcctcc actatctatg atagaggagg tctttttaaa
    14281 atacttaggc ataccggtgg ctgtcacttg gtttatgtcg caaaagacgt ttgatggcaa
    14341 gaaaccactg ggatttttac gatcctttat cagctatcac ttgcgattta aggtgacttt
    14401 tgcaggaaag aaagtcaaag aacagaagaa acgatgggat gaacccagta ctcttgtaag
    14461 gagtgtgaac tatgtatccg attaagtata ttgaaaataa tctcgtcttt aaccaagaag
    14521 gcgagtgttt tgcctactat gaattagtgc cttacaatta ctcctttttg tcacccgaac
    14581 agaaatttca agtgcatgat aactttcgcc aactaattgc gcaaaatcgt gaaggcaaga
    14641 tccatgcgtt acaaattgcc acagaaagta gtattcgcgc cacacaagaa cgatcaaaaa
    14701 aagaaattac cggtagactc aaagaagtag cgaaacaacg aattgatata cagacagatg
    14761 cattagtatc catgattggc gatagtcaga ttgattatcg cttttttatt ggctttaaac
    14821 taattgcgac agatgaagaa gtcaatctaa agagtctcaa gaaatctttc ttttctgggc
    14881 ttcaagaatt tgtctatgga gtgaatcatc atttgatggg cgattttgtt tccttatcca
    14941 atgaagaaat tcgctgctat tctaagcttg aaaaattaat ggaaagcaaa ttggctcgaa
    15001 gatttaaagt caggcgagtc acaccgagtg acctgacgta tttgattgaa catatttatg
    15061 gcgaaaaagg gataccgttt gaagaatacg aatttcagct accaaagaaa aagctaaaat
    15121 cagaaacact ggtgaaacgt tatgacttat tacgtccaaa tcgttgttta attgaagaaa
    15181 aaccaaggtg cttacggatg gaacatgaga atcatgagtc gtacgtagcg tatttaacaa
    15241 ttaatacaat tgtgggggag atggaatttc cttcctcaga acttttctat taccagcaac
    15301 aacaatttac attcccaatc gacacaagta tgaatgtaga gattgtgacg aataaaaagg
    15361 ctttagctac cgttcgtaat aagaaaaaag aactgaaaga tttagacaat catgcttacc
    15421 aatctgacaa tgaaacgaat tccaatgtct tagatgcttt ggattccgtg gatgaattgg
    15481 aaaccacctt ggatcagtca aaagaatcta tgtacaaact gagttatgtg gttcgagtga
    15541 gtgccgaatc agtggatgaa ctaaagcgac gctgtgatga agtgctcgat ttttatgatg
    15601 ataccaatgt aaaactagtc cggccctttg gcgatatgat ggggctacat gaagaattct
    15661 tacccttaag taaacgatat atgaacgact atattcagta tgttacgtct gattttttgg
    15721 cggggcttgg ttttggggct actcagatgc taggagaatt agaagggatt tactttggct
    15781 acaatgtgga tacaggtcgt aatgtttacc tgaaaccggc attggcttca caaggagtca
    15841 agggttcagt aaccaatgcg ctggccgccg ctttcctggg ttctcttggt ggaggtaaat
    15901 cctttagtaa caatctctta gtctactatg ctgttttgtt cggtggtcaa gctgtgattg
    15961 tcgatccaaa agggggtgcg aaacgttcct acttgaaaag agtaggcaca gcacttcatt
    16021 aaatcaatat ttaatgtgtg ttggagaact aaacacacga atagtcgaat gacggggtaa
    16081 cgccctgaaa ggctacttta atactctgac tggcagaaac ttgagtaaac aaggctctgt
    16141 cagaagctcg gtgaagtcgg cagacagaca ccgtaacaga taatgctgac gaaagattcc
    16201 cagaggtggg cgttgtgtcc tatatgccgg aagtctataa aatatctatg gttagaatgt
    16261 agatgagagg tctactgacg aatccttgaa tgtacaggtc tatatcgggg ctggatagaa
    16321 atgtccagtg acacaaacag tgtcgctact gtgggtgagt aagactccgc atttatgaaa
    16381 atccatataa cgttaaaggc gttatcaagg tagcaggctt atagaggaaa cctaaggata
    16441 tatgtacaga tagtgtgttt ggaacgttga aagctgacaa catggagatt ttactgtctg
    16501 tgaagtgttc acatcagaaa gttattgtga gattagcaat gatataaggt gtgttaatgt
    16561 cagtgagagt ggtggcacag tacctatgaa accgtgataa caagcggtgg agggatagcc
    16621 actagtcata tgaagaatag aattactaag aaaaatggta tgggttcgag taagactaag
    16681 agatgtactt gctcctgcaa acaaggaggg tacagaccat gctaaagaaa aacaagctcc
    16741 gctataacga gtattatgat atgcaacata tttatgacga gttatacgca cagagcaaga
    16801 acggtaataa cttttataag ttacttgaaa ttattggttc ggaacaaaat atttgtttag
    16861 cttaccgtaa tctgaaatca aacagtggca gtaaaacggc tggaacggat ggaatgacga
    16921 tagacgatat aaaacaactc agcaatgctg agattgttgc taccgttcgg gaaagcctta
    16981 gcaactaccg ccccaaatca gtcagacgtg ttttcattcc aaaagctggg agcgataaaa
    17041 tgcgtccatt ggggattccg tgtatctggg acaggctggt acaacaatgt atcctgcaag
    17101 tcctagagcc gatatgcgaa ccaaagttcc ataatcattc ctatggattc cgtgcgaatc
    17161 gaagtgccca ccatgcagtc agcagagtaa cgacactgat aaatttatca aaatatcatt
    17221 actgtgttga cgtagacatt aaaggatttt tcgataatgt caatcatgga aaactattaa
    17281 aacaaatctg gacacttggt attcgggata aaagattgat ttgcattatc agcaaaatgc
    17341 tgaaagccga aattgacggc gagggtgtcc cagaaaaagg aacgccacaa ggcggtctgt
    17401 taagtccact gctctccctc atagtcctta atgaattaga ctggtgggta agcagtcagt
    17461 gggaaacatt ccagccgaag aaccgcagta aaaatgggtg gcttcaatac gcaaagaaat
    17521 ataccaaact gaaaagcggt tttattgtac gttacgctga tgattttaaa atcatgtgtt
    17581 caacctatgg agaagcacaa agattttacc acagtaccgt agattttctt aataaaaggc
    17641 tgaaattaga aatcagtcca gagaaatcaa aggtagtaaa tctgaagaaa aattcatcag
    17701 attttctggg gttcaaaatc aaagtcattc caaaaggaag aacgaaacac ggttatgttg
    17761 ccaaaacaga tatgaaccaa aaagcattaa agaaagcgaa aacaaatcta aagttaaagg
    17821 taaaagatat agccagacat acgactggct tcaatatcag cagatataat ctgacagtta
    17881 tcggtatgca gaattactac tgcatagcaa ctaacgtata taacaacctg acagaagtaa
    17941 gttacgctct cttaccgacc atacgaatcc gtttaaggaa cattgcaaaa tcagttccat
    18001 ttgaaagtac aagttcagaa tttcagagca ggacaaaagg aatcagacca aaaacaaaaa
    18061 tagtgatgat tgcggataat ccgctattgc cgattcaagg agtacaacac aaaaatccta
    18121 tgaatttctc gcaagatatt tgtaatttta caaagcaagg gagaaacaag gtgcatgagg
    18181 acgttgtagt tgtaacaaaa gaagaaatcc gagcgttgtt ggaaaatgaa aaccctgccg
    18241 atagcgtaga gtttaacgac aatcgtattt cggcatatat cgcacaacaa ggcaactgct
    18301 atgtaatgaa tcgtcgagga actccgtcca ctctgatatg tattcacaaa tcagacaaag
    18361 gagataacct tgacagatat tctaatctgg catttgtgga aattcccatt gcaaaggcaa
    18421 tcctaacaga atcagcagac gaagcaaaat cactgttaaa aggttatgtt cttaatagtc
    18481 agcagaagaa aaaactgaac agaatacgga cgaattacgg ataccagaca ataactggga
    18541 aataacttat aaatctattt ggatatgatg gaacgccgta tgaagcgaaa gtttcatgta
    18601 cggtgtgaag cgggggaaaa cttggagata acatcaaaaa gttacctatc gctatagcga
    18661 ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct caacttgacg
    18721 agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa aaagaaagac
    18781 tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag tcgagacggg
    18841 gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga tcaacggggg
    18901 ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga aaatatcgcc
    18961 gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc agacggctcg
    19021 attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc ggatttggtt
    19081 ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct tagtgtttcg
    19141 atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg agaaatcttc
    19201 aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg aaaaacgtta
    19261 tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt cgtcactcaa
    19321 aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa gtttgctttt
    19381 cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt ggatcaggag
    19441 gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg
//
LOCUS       DAHUVV010000048        19400 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.049, whole genome shotgun sequence.
ACCESSION   DAHUVV010000048 DAHUVV010000000
VERSION     DAHUVV010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.049"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            76..3603
                     /locus_tag="NK767_001679"
     CDS             76..3603
                     /locus_tag="NK767_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HCH6694675.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3600..7322
                     /gene="addA"
                     /locus_tag="NK767_001680"
     CDS             3600..7322
                     /gene="addA"
                     /locus_tag="NK767_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCH6694676.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7391..7732
                     /locus_tag="NK767_001681"
     CDS             7391..7732
                     /locus_tag="NK767_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6694677.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8041..9084
                     /gene="pheS"
                     /locus_tag="NK767_001682"
     CDS             8041..9084
                     /gene="pheS"
                     /locus_tag="NK767_001682"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCH6694678.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9089..11509
                     /locus_tag="NK767_001683"
     CDS             9089..11509
                     /locus_tag="NK767_001683"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCH6694679.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11679..12689)
                     /gene="trpS"
                     /locus_tag="NK767_001684"
     CDS             complement(11679..12689)
                     /gene="trpS"
                     /locus_tag="NK767_001684"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCH6694680.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12943..13680
                     /locus_tag="NK767_001685"
     CDS             12943..13680
                     /locus_tag="NK767_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCH6694681.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13694..14509
                     /locus_tag="NK767_001686"
     CDS             13694..14509
                     /locus_tag="NK767_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCH6694682.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14522..15169
                     /locus_tag="NK767_001687"
     CDS             14522..15169
                     /locus_tag="NK767_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCH6694683.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15185..15841
                     /locus_tag="NK767_001688"
     CDS             15185..15841
                     /locus_tag="NK767_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCH6694684.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16003..16824
                     /gene="racE"
                     /locus_tag="NK767_001689"
     CDS             16003..16824
                     /gene="racE"
                     /locus_tag="NK767_001689"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCH6694685.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16827..18182
                     /gene="rph"
                     /locus_tag="NK767_001690"
     CDS             16827..18182
                     /gene="rph"
                     /locus_tag="NK767_001690"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCH6694686.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18183..18701
                     /locus_tag="NK767_001691"
     CDS             18183..18701
                     /locus_tag="NK767_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCH6694687.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18808..19308
                     /locus_tag="NK767_001692"
     CDS             18808..19308
                     /locus_tag="NK767_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCH6694688.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6515 a         3336 c         4170 g         5379 t
ORIGIN      
        1 agaatataga acaaatgttc agtatttttt gatataataa aggagcaccg tatcatgtgt
       61 tgaaggagga aaagcatgtc tttacaattt attcttggaa acgggaaaaa agatcaccgt
      121 caagtcttga tcgacgaagc atccgcttgg ctcgaccagg atgcaagtaa ccaagtattt
      181 ttccttgttc ccaactacag caaattcgaa caggaacaag aaattttagc ggagatgcga
      241 agaagaagcg gaaaaaaaac tttcagcaca acaaacgttc aagtatttag tttttatcgt
      301 ctggcttggt atttgctgca acagacaact cttcttacag gaaatgaatt atcagaatca
      361 ggatctgcaa tgatcttgcg acagatttta gaaaaaaatg aggaagaatt aacaatcttt
      421 cgcggagaaa tcaataaaag agggtttatt cgtcaattac aagaactcta cagtgaattg
      481 cgtacaggaa atattcaacc agaagacctg atgcttttat ttggtgattc acctaatgca
      541 gaagaccagc aacttaaaat gaaagacttg aagctgattt tttcagctta tgacagcgaa
      601 ttgactcaac gtgcactaac aaatgaagat gcactatcta tactagcaaa ttatatgcag
      661 acacaagatt tcaacaacgt caaatttatt gtcagtggtt tttctcgaat caatggtcaa
      721 gagtatcaat tactgcaggc aatgatggaa aaggggcaat tggttgtcga tttattgctg
      781 gatcgtcctt atacagcgga tcttccagaa gtacttacgc tctttcatga gactggaaag
      841 ctttatttcc gtctttttca aagtgcaaga gaacggaaag tccctatatt agtggaccga
      901 tttgcaccag agcagactcg tccgacagag cttcaactgc tgcaaacaat gtgggaagag
      961 acatctagcc aaaagaaagc ggcagtacac aaaatagggg gacaagagca tctccatttg
     1021 tgggaagctg aaaatccaac tgaagaagtc cgacaattag ctgttgaaat cagacgacta
     1081 gtcagtgaag aaaactaccg ttatcgtgat atccaagtgc tgacaaaaaa tatgtcttta
     1141 tatggaaata tccttcggcc gatctttcaa gaaatggcaa ttccttttta tatcgatgaa
     1201 gaacaaatga tggaaaatca tccgctgatc gaatggatca acagtctgtt cgctcttgac
     1261 cgctattctt atcggctaag tgatattatg cgttttttca aaactgaatt atttctgtcg
     1321 acggaagcag aaaccgaact tgatgaatgg aaacaagagc gagatgaatt caggaaaaaa
     1381 ctggacatca ctgaaaatgt agcacttgct tacaattatc aaggaactta ttggactcgt
     1441 gaaaaagact ggcagtttgt ggcgtatgat tttgaagcag aacagattga agacgtacag
     1501 tatttggaag aacagtcaaa tacgattaga cgattatttc agaaagctgt accagtattt
     1561 ttgaaaaaaa taaagaaaag caaaacagga caagaggccg caaatgtttt ttatcaattt
     1621 ttgatccaaa gtggcgtaca gcaacaatta ttgttttggc gtaatcagga agtagacaga
     1681 ggaaacttag aagcggcaag aaaccatgag cagacatggg gagcgctgat tgatctgttg
     1741 gatgaatatg tagtaattta tggtgaatcg gcttttgact ggtcactgtt ccaagaaatc
     1801 attacaagtg gtctagaaaa tctaagttat ggtaaaatcc ctaccgcaat cgaccaagtg
     1861 cgaatcaatc gtttggaatt agttcgacca aatcaagctg ccgtcacttt tgcattaggg
     1921 ttgaatgatc aagtgtttcc agaccgaaaa gagacaaaag gattgttgtc cagtgaagaa
     1981 cgagaagtat taaatcaggc cttgccagat ggacagtttt tattcgaccc atcaaaggaa
     2041 agtatctctt ttgagccttt ccaagcctat cttgtttttc tttccggaac agatcgatta
     2101 tatcttagtt atgcacaaag ctatgacaca gatggttcgc tgaagatgtc tccttatttg
     2161 cgtagaatca cagaatattt atctgttccc gtcgagagaa aagagcattt gacgatcgaa
     2221 agcgacccta attgttatgt agggacttac agaagcggaa tcaatacagt caataggatt
     2281 tatcgattag ctttagatga aaaaaaacct gtaccaaagc actggcaaaa gctcaaagaa
     2341 ttagtactga attcgtcatg gcgaaatttt gctttacaga tttttgaaag tcaggaacat
     2401 ttaaatgttc ctgtttcgat gccagaaaaa atggcggaag agctttatgg aaaagagatt
     2461 tatacttcaa tttcacgtat ggagaatttt tataattgtg aatacaaata cttcatgcaa
     2521 tttggtttga aattgaaaga acgaactgtt tatggtctaa ctccagcagc aactggtgat
     2581 ttttatcatg aatcattgga tcgtttcttc aagctactgt tttcaaacca attatcctta
     2641 gtgaatatgt ccgatgcgca gcgtagagac tttactgaaa aagtgctgca ggaagtattc
     2701 ggagaacttc ggtttgagat attggatagt tctgcaagaa tgaactatat ccgctaccag
     2761 ttaggacaga cgattcaaaa agtgacatgg gctttgcaaa aacagagcaa aaatacaggc
     2821 atgcatcctc ttcagacaga gattctattt ggtcagattg ctggagcaaa aggtatcaca
     2881 ggtctggaat taccgttgag taatggtgga aaactccatg ttcgagggaa aatcgatcga
     2941 atcgatgtag cttctgaaca agaagatact tggctaagcg tcgttgatta caaatctagc
     3001 ggacgttcat tcgatgtcac agaagcttat tatggcatgg ctatgcaatt attgacgtat
     3061 ctggacgtag cattgatgga tgcggtacgt ttgactggaa aggcggctgt taaaccagca
     3121 ggatcatatt atttacatgt tcataatcct gtattgacgg agacagatga tgtagagaag
     3181 aatacattga aaaaatttag ttatgatggc gtttttgtcg atgatcccgc tcttttagaa
     3241 gtactagatc attcactgga agcaaaagaa caatcactga tctatccagt gaaaaaaaat
     3301 gcgaaagatg aataccaaaa aggtagtttc agtaaagaca aatttttcac ggaagaagaa
     3361 ctgcaactat tcatggcgca taatcgggaa aatatgcggt tagctggaga tgcgattact
     3421 tccggagaaa tcaaactaaa tccagcttat aaagataaac agcggattgc ctgcgaattt
     3481 tgtccattca gaagcatctg tacgtttgat gtgatgctga aagaaaataa ttaccaccgt
     3541 ctggagaaaa tagataagga agaagcactg cgtagaatat tgaaaaggag tgaagaagag
     3601 tgaatcaaat tcctttaaaa ccgcaaaatg aacgattcac agatgatcag tggcaagcga
     3661 tttttgatca aggagataat ctattagtct ctgcgtctgc cggatcaggt aaaactacag
     3721 tgcttgttcg aagagtgatt gaaaaactaa aaatgggttt tgatatcgat gaattattga
     3781 ttgtgacatt tactgaagct gccgcaagag aaatgaaaga aagaattcaa gaggcattac
     3841 aagaatccgt caatagcgaa agtgaccctg tacgtagaca gcattttact aagcagttag
     3901 ttttattgcc cacagcgaat atctcaacct tacatgcttt ttgcctgaca gttattcgac
     3961 gttattatta cttaattgat attgatccag tctttcgcat gttaacagat gaaacagaaa
     4021 cgatcttgat gaaggaggat gtgtgggacg agctgagaga agcactttat gcagaaaatg
     4081 atgaacgatt tttccaatta acaatgaatt tttctaatga tcgttcagat gacggcttga
     4141 cgaatctggt gttttctctt tatgagtttg cccgtgccaa ccctgacccc caaaaatggc
     4201 tagaacagtt aagcgataat tatcgtttgc ctgaggggct agcgaaatct cgattatatc
     4261 aaaaacaaat ccgtccttta gttttggcag atatatacca atgtgtacag ttatatgaac
     4321 agatgactca gttagcacaa ggtgaaggat tagaaaaaat gcatgagcaa gtagctggag
     4381 aacaacagca gatcaagaat atctatgaag cgttctcgca agatcggtta gaagaagctt
     4441 atgctggttt ggaacaacta actttttcaa cttttaagag cagtcggaaa gcagaattga
     4501 aagaaatttc caatgaagtc aagggcatga gggataaagc aaaaaaactg attcagcaga
     4561 taagtaaaag ttattttcct gtttccccaa gccaaatgga agaattaacg gacaaagcac
     4621 taccgttagt cgaagaaatg acaaaagtca cgcaaagctt catggacggt ttcagtatgc
     4681 gtaaacggga aaaaggtgtg ctggatttca atgatctgga acatttagcg ttgcagattc
     4741 tcacagaaaa aacgaaagat gtatggctgc caagtgaagc ttcaaaacat tatcgaaaaa
     4801 aattcaaaga agtaatggta gatgaatatc aagacgtgaa ccaattacaa gaagccattt
     4861 tatactggct aagagaaccc gatgatacaa aaggaaatat gttcatggtt ggggatgtca
     4921 aacaatccat ctattctttc cgtttagcag atcctagttt atttatcggg aaatacgaga
     4981 atttctcaaa gaaagaaggc ggacgtcgaa tcgttttggc tgagaacttc cgctctagaa
     5041 aagaagtcct ttcttttacg aatcttattt ttgaacaact aatggatcca gcagtgggtc
     5101 aaatcaatta tgacgaagca gccaaactga tccaaggatt ttcagacttt ccagagaatg
     5161 aacagtttga gccggaaatc atgatctatg aaaaagaaca ggaagaatca gagattgaga
     5221 ttccgacgga tgatatactg gaagacaaaa cagaaggcga actttttatg acaggactaa
     5281 aaattcgtca attaattgat tcctccttta tgatttatga taaaaaatca aaaaaaagtc
     5341 gtccgataga gtacaaagac attgttctct tgacacctac aaagaagaac aacttaacga
     5401 ttttagagat tttcaaaaca ttggatattc cattagagat gaatgatgct caaaactatt
     5461 ttcaagcgac agaaattcgc acgatgatct cgctgctaca attaatcgat aatccctatc
     5521 aagatattcc gcttgcggct gtattgcgct caccgatcgt gggtctgatt gaaccagaat
     5581 tagccagcat tcgattagct aaccgtgcac atacttacta tgatgctgtg cttgcttatc
     5641 aagcatcaaa cgaagatgaa ttagcagcta agctggaaca ttttggcaag caactagagc
     5701 attggcgaga acttgctcgc cgttcttcga tcactgactt gttatgggat atctattatg
     5761 agacgggata tctagagtat gtggtcggtc tgccagcagg ggcacagaga caagccaatc
     5821 tttatgcatt ggttgatcgt gccaaagctt acgagcaaag ttctttcaga ggattatacc
     5881 agttcgtgcg tttcattgaa aaaatgcaag aaaaagataa agacttagca gaaccagtca
     5941 tttcaataga agataatgct gttcgcgtta tgacgatcca tgcaagtaaa ggcttggaat
     6001 tcccagttgt atttttgtta gatatgacaa aggagttcaa cttacaagac ttgcgcaatc
     6061 ggtatgcttt tgaagaaaaa cttggagcag gaattcgcta tatggatcct gaaacacgag
     6121 tgttatatga tacgttaccg tttcaagcca tcaaattggc aaagcaaaac aaattgttgt
     6181 cggaagaaat gcgtaaattg tatgtaggat tgactcgggc agaacaaaag cttttcattg
     6241 tcggttccta taaaaataaa gagcaaatga tccaaacgtg gtctgaagca gctgatcatg
     6301 aggagttagt ttttgatcct gcgctacgtt tgaagggcag aagcagcttg atgaattgga
     6361 ttggttacgg attgatccgt catcctgaaa tgcagaaata cttagaggaa gaaatttcga
     6421 cttctcttct tcaacattca aatgcacagt tttctatttc atggatgaat cagcaatcga
     6481 tcattgaaca aagacagttg cttgctgaaa aagaactggt taacttagat cagcagatga
     6541 aagaagatga aactttatta gcagattcgt tacaaaaacg tctggcatat gaatatcctt
     6601 atcaagcttc ttcacaaacc acaagttatc aatctgtatc tgaaatcaaa cgattattcg
     6661 aagatccaga tgatacacaa gaatcacgtt tgactctgga aagcagtcag aacaaagcag
     6721 catcccgtca atttcgttat acgcaagaac aactagcaga accaaaattt ttgcaaaaag
     6781 atcggcaagt gtcagcagct gctgttggaa ctgctacgca tgctttgtta caattactgc
     6841 cattagagat gccaactaca gagagtatcc atcaaaaatt acaggaattg gtaaggaaac
     6901 ggttagtaga cgaaaaagtc gcgaaaaaag tagatgtttc ttctattata tggttctttc
     6961 aaacagaact tggtcaacag ctgattgcca acaaagaaaa cgtaaaacga gaacaacctt
     7021 tttccatgct gcttcctgct gacgaagtat ttcaagacta tccaaatcaa gaagatgaac
     7081 tattaatcca cggaatcgtc gatggatacc tagaagaaaa agatcatctc aatatctatg
     7141 attttaaaac agattttatt ttacatccgg atgatccagc agaaatagat gcaatcgtcc
     7201 aaaaatatca aggacagcta agactttacc aacaagcaat gtcagaagct ttgaataagc
     7261 cagtagaaaa tgttttccta attttgcttc gagtgaaaca aattataaac attaacaaat
     7321 aattataatt gagcaaactt gacttatgtt ttgtaaggat tatgctatac tctgggaaga
     7381 ggtgagtatg atggagctaa cacagacaga attcgtttta tgccctaaat ttgaaaaaac
     7441 attctctatt ttaggaaaaa aatggaacgg attgattatc gatgtactac tcgaaaacgg
     7501 tccgcaacgc tttgttgagc ttgcggcaaa aatcccgatg gtcagtgatc gagtattagt
     7561 cgaacgcttg aaggaattag aaaaagaagg aatagtcact cgaaactttg cctgtagcga
     7621 aaagagccgt tcagaatatc tcttgactga aaaaggagca gacttgcaaa aagcgatgcg
     7681 tcaaatacaa gtttggagcg aaaaatggat cacagaggaa gaatgcggtt gattttgtaa
     7741 aaaaaatctt gtaaactata gtccatacga ataagtaaag aacgatgacg aaaaagagta
     7801 gtttggctaa accagttcag ggagatctgt cgcagactga aagcagatcc tgtgtttgaa
     7861 aaatgaagtt cactttccga gttcgtctga taaaatcaga ccggcaattg tcgttaacga
     7921 agtaagagtg cggtgtaagt catttatccg caaattagga tggtaacgcg aagacctcgt
     7981 tccttaacag gaatgaggtc tttttgtttt gtatttggaa tttaaaggag gaacaagcac
     8041 atgtctctac aagcacaatt agagagcttg aaagaagcaa cgctcgtcaa aatcacagcg
     8101 gcacaagatt taaatgaatt aaatcaaata cgtgttgaaa cgttaggaaa aaaaggaccg
     8161 atcacagaag tattacgtgg gatgaaagat ctaagtccag aagagcgacc aaaagtcgga
     8221 agtttcgcaa atgagatccg agatcttttg acagaagaga tcgtgcgaaa aaaacaagta
     8281 ttagaagaag aagcgttaaa tgcagctttg gcaaaagaaa caatagacgt gacactacct
     8341 ggtagaaaag taaaacatgg tacacgtcat atcgtgacac aaatcatgga agaaattgaa
     8401 gatatttttg tgggtatggg ctatcagatc gtagaaggat atgaagtaga atctgatcat
     8461 tacaatttcg aacggatgaa tctgccaaaa gatcatccag caagggacat gcaagatact
     8521 ttctatattt cagacgagat attgattcgg acacatactt caccagtcca agcacggaca
     8581 atggaaaaac atgatttctc taagggtgct ctacgaatga tctcgccagg gaaagtcttc
     8641 cgcagagata cagatgatgc gacccacagt catcagttcc atcaaattga agggctagtt
     8701 gttgataaaa acatcacaat gggcgacctt aaaggaacat tagaagtcgt aatgaaaaaa
     8761 atgtttgggg aagatcgtga aattcgtttg cgtccaagtt atttcccatt tacggaacca
     8821 tcagtagaag tagatgtcag ctgtttcaaa tgtggtggtg ccggttgtaa cgtatgtaaa
     8881 tacactggat ggatcgaaat tttaggagct ggcatggtac acccgaatgt gttgaagatg
     8941 tcaggaatcg atccagaaga atattcaggc tttgctttcg gccttggacc agaccgcgta
     9001 gctatgttgc ggtatggagt gaatgatatc cgtaatttct atcaaaatga cttacgtttc
     9061 ttaagtcagt tcaaggggga gtaagaaaat gttagtttca tataaatggc tcaatcgtta
     9121 tgtagattta tcgaatgtaa caccaaaaga attagcggat aagatgtctg tgacaggaat
     9181 cgaagtagag ggagtaacgg ttccagaaga aggcttgaaa aaaatcgttg tcggtgaagt
     9241 caaggaatgc gtaccacatc cagattctga tcacctttct atttgtcaag tcgatattgg
     9301 cgaagacgaa ctgtctcaaa tcgtttgtgg agcgcctaat gtgaaggttg gaatcaaagt
     9361 gattgttgca cttcctaact cacgtatcgc tggtaatgta aaaatcaaaa aaggaaaaat
     9421 gcgtggccaa gtatcaaatg gcatgatttg ctcattacaa gaaattggct attcagatag
     9481 tgttataccg aaagaatacg ctgaaggtat ctattatatg ccacaagaag ctgtaaatgg
     9541 cgatccagtt tttccttatt tggatatgga tgattcgatt attgaattat ctatcacacc
     9601 gaatcgtgcg gatgcattgt caatgcgtgg cgtagcttat gaagttgggg caatttatcg
     9661 tcagactcct acattcgatg acgaaaagct ggtagaatcc tcacgtcctg ctagcgataa
     9721 aattcaagtg gaagttgaag ataaagaagc agttcctgct tatcaaatca gaatcattga
     9781 aggagtaaga atccagccaa gtcctcaatg gctgcaaaat cttttgatga acgaaggcat
     9841 tcgtccaatc aacaatgtag tagatgtgac gaactatatt ttgcttttat tcggacaacc
     9901 actacatgca tttgactacg acaaattgaa atcaaaagag atctttgtcc gccgtgcaaa
     9961 agaaaatgaa acattagtaa cactagatgg agaatctcgg gaattgacaa ctgacaatct
    10021 cgtcattact aacggcaaag aaccagttgc tttagctggt gtcatgggag ggttagactc
    10081 agagatcacg aatgaaacaa caacagttgc actagaatct gctttatttg atccaatttc
    10141 gattcgtcgt acagcaaaac aatttaattt gcgtagtgaa tcctctgcac gttttgaaaa
    10201 aggaatcaac catgcgactg ttggagaagc cagtgaggtt gctgcagcaa tgatcgtttc
    10261 tcttgcgggt ggggaagttc tacaaggcgc ggtcaaaggc tcagagatca aagcagaaga
    10321 cgtatcagtg aagatttcac ttgaacgcat caatcaatat ttaggaacat ctcttgtcgt
    10381 taaagaagta aatgagattt ttgaagcact tggatttagc tatgaagaaa aggagtatgt
    10441 ttattccgta gtagtaccac caagacgctg ggatatccaa atcgaagcag atattatcga
    10501 agaagttgct cgtatttatg gatacgacca gttgccatct acacttccaa gtggagaaac
    10561 ggttgctgga agcttgtcaa aagaacaaga aactactcgt aaaattcgta caatctttga
    10621 aggaagcgga ttgagcgaag caatcagtta tgcattgaca acagaagaaa aatcaagaca
    10681 gtttgttata aatgaaagta atattacacg tttggactgg ccaatgagcg aagaacgttc
    10741 tgtattacga atgaatttga tcagcggact attagatgat atcagttata acactgcccg
    10801 aaaaaataca gagattgctt tatatgaaat tggtcgggtg ttcttccaag aaaatgatcc
    10861 acgtacacat ctgccgcaag aagtaaaaca tgcagcaatc gctatttctg gtatttggga
    10921 agaaaaagat tggcagacga aagctcaaaa tgtagacttt ttcaccatca agggtctttt
    10981 agaaaactta tttgaacaac taggcatcac tgaagatgtc cattatcaaa cagtgacaga
    11041 aatgaaggaa atgcatcctg gaagaactgc tgcgatttac cttggcgaaa aatttattgg
    11101 ttttgtcggt caagtgcatc caacgattac aaaaagttat gatattccag aaacatatgt
    11161 tgctgagttt gatttatctg ctatagtgga agctgctcaa aaagggatcg ttttcgaagc
    11221 agtgacaaaa ttcccagcag tcagccggga catcgctttg cttgtaaaag aaactgtgac
    11281 aaatcaggaa ttaacagaag ttatccgttc tgctgccgga agatttttaa cagatatcca
    11341 attatttgat gtctatcaag gagaaaacat tgaaaaagga cataaatcca tggcgtatag
    11401 cctgacattc gtcaacccag aagcaacatt gacagatgaa gaaatcaata aagcaatgga
    11461 aaaagtcgaa aaagcgttgg tagaaaacct agaggcaagt attcgctaag aaggaaaata
    11521 tagtaagtaa cgatcaagta gcttttgaag accatacatt tgaaaaaaag atgtgacaca
    11581 acttcgtcac atctcttttt ttgattttaa aaaaggaata gcacagagac gagataaaaa
    11641 aacaggcctt tacagcctgc ttttttagag taaatgaatt aatggaagta attgattccc
    11701 attgctgatt ttacttcaga aagtgtttgt gcagctactt tagcagcttc ttcactacct
    11761 ttacgtaaga tttccataac agctgaagga tctttttcca attcttctcg gcgcgcacgg
    11821 atcggtgcaa attcagcttc caatacatca atgaggtatc gtttgatctt tacatcacca
    11881 agtccgccgc ggcggtaatg agctttcatt tcttcaattg cttctttatc tgttccgaat
    11941 acatccaagt aggtgaagac catattccct tctacttgtc caggatcttg tacatggatg
    12001 tggtttggat cagtatacat actcatgact tttttctgta agacatcggc aggatcggag
    12061 atataaatac cgtttcctaa cgatttactc attttgccat ttccatcgat tccaggtaat
    12121 ctacccattc cttttggtgg gaatacgcct tttggttcca ccaagacttc accatatgta
    12181 tgattgaagc tttgtacgat ttcctgtgtc tgctctagca ttggtttttg atcctcacca
    12241 acaggtacaa gattcgcttt gaatgcagtg atatctgctg cttgagaaac aggatagata
    12301 aagaatcctg taggcacgct ttcaccgaat tttttttgtt cgatttctgt tttgacagtc
    12361 ggattgcgcc gaacgcgtcc tacgctgact aagttcaaat aatacatggt taattcagct
    12421 aattccggaa tttgtgattg gataaataaa gtagattttg caggatctaa tcccacggct
    12481 aaatagtcta aagcaacttg caagacattt gatgaaacct tctcaggatt tttagcatta
    12541 tctgtcagtg cttgcatgtc agcaatcatg acaaacagtt ggttgtcttc atctgcctgc
    12601 atagccactc gggtttttag agaaccgacg taatgcccta aatgcagctt cccagttggt
    12661 cgatctcccg ttaaaataat atttttcaaa aaagacacct ctttacgatt tgtaagtctg
    12721 agcacttaat atcttaccgt ttttcctgaa aagaaacaac agttcctaag gaaaaaacaa
    12781 ggaaagagga taaaataact aatagattag aaatggaaga aatatttatg ggagaataaa
    12841 cgttctaaga aactttacaa attactaaac tatcagatat aatgtatttt attatacatc
    12901 tttaaaagaa atcttactaa ataataatgg gagtgtaatt ctatggcaat gattaaattt
    12961 gatcacgtgg ataaatatta tggcaagttc cacgcattaa aaaatatcaa tctcgaattc
    13021 gagaaaggag aagttgtcgt tgtcattggt ccttctggtt caggaaagag cacgatgctt
    13081 cgctgtatca atggattaga gacgatctct tcaggtaagc tgctgattaa tgatactgac
    13141 ttgcatgata aaaagacaaa actgacagaa gtccgaaaaa acatcggcat ggttttccaa
    13201 catttcaatt tgtacccaaa taaaactgtt ctggaaaaca tcaaattagc accaatcaaa
    13261 gtgttgaagc aagaccaggc aactgcagtc aaaaacgcgg agaaattttt gaaaactgtc
    13321 aatatgttag ataagaaaga ttcttatcct tccatgttat ccgggggaca acagcaacgg
    13381 gtggcaatcg ccagaggttt agcgatgaat cctgagatgc tgctgtttga tgaacctact
    13441 tctgctttgg atccggaaat gattggtgat gtattagacg ttatgaaaaa attggctcat
    13501 gacggtatgt caatgattgt cgtgacacat gaaatgggct ttgccaaaga agtggcggat
    13561 cgtgttatct tcatggctga tggagaagta ttggaagaca gcagaaatgt gagagacttt
    13621 ttcgaaaatc caaatgaagt ccgagcacag caatttatca gtaaagttat caatcattga
    13681 taggaggaaa aagatgcgca ggttaaaaaa aatacgcctg attttccttt taggcattgc
    13741 ttttcttgca ctagccggat gtaaaggaaa tgggttggca aatgaagata ttttagagcg
    13801 aagcaaatct accaatgaaa tcatctgggg tgtcaagtat gatactcgat tatttggaat
    13861 gatggatatt gagagcagaa ctgtacaagg atttgatgta gatattgcaa aagcaatcac
    13921 taaaaagatc cttggtgaca atggaaaaac agaatttgtt gaagtcactt ctaaaactcg
    13981 gattccatta ttgaaaaacg gaaatatcga tgcaattatt gcaacgatga cgatcacaga
    14041 tgaacgaaaa aaacaggttg acttttcaga tgtctatttc gatgctggcc aagctttgtt
    14101 ggtaaagaaa ggaagccaaa tcaaaagtgt agatgatttg aatgcttcta caacggttct
    14161 agcagtcaaa ggttctacat cggctgcgaa tatccgacag catgcaccag atgcaaaaat
    14221 tttagaatta gaaaactatg cagaagcatt tactgcttta caatctggtc agggagacgc
    14281 gatgactacg gataatgcga ttttgttagg gattgcagat gaaaatccag agtatgagtt
    14341 agttggtgga acgtttacta atgagcctta cgggattgcg atcaacaaag ggcaagaaaa
    14401 cttcttgaaa gctgttaacc aagcgctaga ggaaatgcat gcagatggca catacgataa
    14461 aatctatcaa aaatggtttc caaatgaaac tgaaggaaaa gtagagtaag ggggagaggc
    14521 aatgagcgaa ttgatacaaa cttatggacc aatctttgtc gacgggttca agatcacatt
    14581 gttttcgagt gtcttagcac ttgtgtttag tttgattatc ggaacgttaa tggcaatctt
    14641 ccaattatca aagaataaag tagtgagcgc cttagcaaag gcatatgtag aattttttag
    14701 aaacattcca ttattaatca tcgtgatgtt tttttatgtt gtcgtaccga tttattggtt
    14761 taatctagat ggattccaag caggtacgat tggattaacg atctatacat ctgcttttat
    14821 tgctgaaaca gtacgttcag ggattcaaac cgtaccaaaa ggtcaaacag aagcaggact
    14881 atcttctggt tttacctatt cagaaatcat gcgttatatt gttttaccgc aagcattcaa
    14941 aatcgttatc ccgccattag gcaaccaatt tatcaacctc gtaaaaaact catctatttt
    15001 agcaatcgta gctggtttag atttgatgta tcaaggagac ttgatcgcta gtgaaacatt
    15061 caataccttc gatacgtata tcatcgttgg gttattttac ttagttatca cgctaccgtt
    15121 atcttactta atggtttatc ttgagaaaaa atgggcggtt cgttcataga aggaggggaa
    15181 atacatggat tttagcggcg ctttttcatg gatgaatatc cgttttttat tggaaggatt
    15241 aaaggttact gttgaagttt cattgttttc gatcattttt agtttcttga tcggtggtct
    15301 gacaggtgtg attcgttttg cgaatatccc tttcttttcc aaaatccttg gtgtagtgat
    15361 tgatattata cggaatcttc cattattatt gatcattttc ttcacttact ttgccttacc
    15421 tcaaatcggc attcagatga acatcttctg gtccgctgtc gcagcattaa cgatttttga
    15481 gtcagctatg ttgtcggaaa ttttccgagc tggactcaat gctgttccaa aaggacagat
    15541 ggaagcagga ttatcaacgg gactgactta tgtagaatcg atgcgaacga tcgtgttacc
    15601 gcaagcattt aaatcaatga tcccggcaat tgtgagccaa ttgatttcac tgatcaaaga
    15661 tacttcatta gcagtaatca tttctttacc tgaactttct catcaggcac gtattattta
    15721 tggacagaac accaattatg tgcttccgat gttcatcatc atgacactta tgtacttcgt
    15781 ggtatgttac gcgctttctc tgctttccgg ttacttggaa aagagacggt acagttatta
    15841 atagatgatt cttttagcgg ttgtggcaaa ctaaagtcac aaccgctttt cctctttcca
    15901 ttcgaaaaaa agagatttcg attgttttaa gcaagtaaga gcacttgaaa attcacgcta
    15961 aaaagtacac aatagggagg atagaattta ttgaaaaaaa ggatgatacg attgacagat
    16021 aatcgcccta tcggatttat tgattcaggt gtcggcggct tgactgtagt aaaagaagcc
    16081 ctgaaacaat taccgaatga aaatatttta tttgtaggag acacagcacg ctgcccatat
    16141 ggccctagac ccgcggaaca ggtaatccag tatacttggg aaatgacgga ttatctggtg
    16201 gagcaaggaa tcaagatgct ggtgatcgcc tgcaataccg caactgcggt ggctttagaa
    16261 gaaatcaaag ctgctctttc tattccagtc atcggtgtga tccttcccgg tactagagcg
    16321 gcagtaaaaa aaacacaaaa taaacaagtt ggcattatcg gtacgattgg tacggtaaaa
    16381 agtcaagctt atgaaaaagc actgaaagag aaagtaccag aattgactgt gacaagtctt
    16441 gcttgtccca aatttgtttc agttgtcgaa agtaatgaat accattcatc ggtggcgaaa
    16501 aaaattgtgg cagaaacatt agctccttta accactaaaa aaatcgatac attgattttg
    16561 ggatgcaccc attatccatt attacgcccc atcattcaaa atgtaatggg agaaaatgtt
    16621 caactgatcg attctggagc agaaacagta ggtgaagtat ctatgctgtt agattatttc
    16681 aatttgagca attcaccgca aaatggtcgg acattatgcc agttttatac aactggctct
    16741 gccaaacttt tcgaggaaat agctgaagac tggcttggaa tcggacactt aaatgtagaa
    16801 catatcgaat tgggaggaaa ataaggatgc gtcatgacgg gagaaaagcc caagagcttc
    16861 gcaaactgga gcttcaaaca aatgttttca aatatccaga aggatcggta gtgatccgtt
    16921 ttggtgatac aacagttatt tgttctgcaa caatagaaaa ttctgttccc ccttttttga
    16981 gagaaacagg aacaggatgg gtgacagcag aatatagtat gctgcctcga gcaacgagta
    17041 cgagaaatcg tagagaaagc agcaaaggga aactttctgg tagaacaatg gaaatccagc
    17101 gtcttattgg tcgttccttg cgtgcagtag tagacttgga aaaattagga gaacgtagta
    17161 tcatcgtaga ctgcgatgtg atccaagcgg atggcggtac tagaacagca agtatcactg
    17221 gggcatttgt ggcattgaaa ttagccattg aaaaactttt aagagaaaaa gaattgtcag
    17281 aagatccaat aaaagaacat cttgctgcag ttagcgtagg aatcttacca gatgggactt
    17341 gcgtcacaga tttggattac caagaagatt ccgcagcact tgtagacatg aatcttgtaa
    17401 tgacagaatc tggaaaattc gtggagattc aaggaactgg agaagaagca acgtttgatg
    17461 gtgaacagtt gaatgaaatg ctcttttttg gaaaaaacgc gattgaagat ttgatcaaag
    17521 agcaaaaaca tgctttgcta acagagtttg ctcaaaatga tgagcggata gaagaaacaa
    17581 aaacaatcat cattgcgaca agaaatccag gaaaagcaga agagtttcga aatatgttca
    17641 aagaagcggg atatcatgta aaaacattac ttgactatcc tgaattgcca gatgtggaag
    17701 aaactggcag cacatttgaa gaaaatgccc gattaaaagc agaaacgatt gcacaattgc
    17761 ttgatcagcc agtcttagca gatgattcag gtttgaaagt ggatgcttta ggcggtatgc
    17821 caggaatcta ttctgcacgt tttgcagggg aacaaaaaag tgatgcgggg aataacgcca
    17881 aattattata tgaattgaca gatgtgccgg atgaaaaaag aacagcacaa ttccattgta
    17941 cgttagtatt cgcggcaccc aaaaaagaca gccttgtagt agaagcggaa tggcccggtc
    18001 gagtagcccg tataccatct ggcgaaaatg gctttgggta tgatccttta tttatcccag
    18061 aaggtaagaa acaaacagca gcagaattat cgagcgaaga aaaaaacaag atcagtcatc
    18121 gggctcaagc aatgaaaaaa ttgagtgccg agtggaaaca atggctggaa ggagagagat
    18181 agatgcggta tttagtagta agtgataatc atggagacag agatatcctc gttgacttag
    18241 tagatcgtta tcgttctgaa gtggatatct ttttccattg tggtgattcg gaattagaag
    18301 cttctgaccc attatgggat acattccaag tggttcaagg taattgtgat tatggaccag
    18361 gattcgaaca gaaaaaagtg atccaaactg gtcaggatac agtattcatg actcacggtc
    18421 atttatcgaa tgtccgattt ggtttgacac agttagcaat cgaagctcga gctgcaactg
    18481 ccgatatggt gttttttggt catacccatc aaataggctg tgagatggat cagaaaatcc
    18541 tgtttttgaa tcctggtagc atttctcagc ctagaggccc gatccaaatc ccatcatatg
    18601 ctcttatcga aagtttagat gaccaaattt ctgttcaata ctataaccgt gcccatcagg
    18661 cagttgaaga gttggccttt tctttttcga gagagcgctg aataaaaaaa ctctaatttt
    18721 taataaaaat aagctttatt ttatgaattt tgcatccggt ttcggtaaaa tagtagtgtt
    18781 gaatgaaata gaaatggagg ccatcagatg attggaccta tagtaagaga aatgctgctg
    18841 gaaaatcagg aaacgttttt gattcctgca gaaaatgtgg caaatgttat gtatcatcat
    18901 ccattgtccc atggtttact tgtgttatcg aaggtcggtt attccaaaat tcctgtatta
    18961 ggaaaagatg atcactttgt aggactagta agtttatcag atgtggtcaa taaaatgatt
    19021 gacttgcaaa caatcagcat ggacccgtta gaaggactaa cggttgctga tgtgacagaa
    19081 acagatgttc cgacaattga tgaaaattgg gaactagaag aagtactcca tttgttagtc
    19141 gattcgcctt ttttaccggt tgtcacagaa gatcggattt tcaaaggaat cattacaaga
    19201 aaagagctgc ttaaagccgt aaacttcatg gtgcatgaat tagaacgtcg taatatattg
    19261 tctccgaaaa tcgaagaatt aaaagaacaa atcgatatcg tgagctaaag aacgagaaac
    19321 taaaagataa tcaaaaaata agtagaaaaa acagcgtacc agttttgact ttatcttgaa
    19381 aaagaggctg ggacaaaagc
//
LOCUS       DAHUVV010000049        18871 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.050, whole genome shotgun sequence.
ACCESSION   DAHUVV010000049 DAHUVV010000000
VERSION     DAHUVV010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.050"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(9..530)
                     /locus_tag="NK767_001693"
     CDS             complement(9..530)
                     /locus_tag="NK767_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCH6694689.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            729..4421
                     /gene="nifJ"
                     /locus_tag="NK767_001694"
     CDS             729..4421
                     /gene="nifJ"
                     /locus_tag="NK767_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCH6694690.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4610..5497
                     /locus_tag="NK767_001695"
     CDS             4610..5497
                     /locus_tag="NK767_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCH6694691.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTRAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5494..6663
                     /locus_tag="NK767_001696"
     CDS             5494..6663
                     /locus_tag="NK767_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694692.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6687..8042
                     /locus_tag="NK767_001697"
     CDS             6687..8042
                     /locus_tag="NK767_001697"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCH6694693.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="NK767_001698"
     CDS             complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="NK767_001698"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCH6694694.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10329..10619
                     /locus_tag="NK767_001699"
     CDS             10329..10619
                     /locus_tag="NK767_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694695.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10632..11657
                     /gene="sppA"
                     /locus_tag="NK767_001700"
     CDS             10632..11657
                     /gene="sppA"
                     /locus_tag="NK767_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCH6694696.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11660..12319
                     /locus_tag="NK767_001701"
     CDS             11660..12319
                     /locus_tag="NK767_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCH6694697.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12476..13408
                     /locus_tag="NK767_001702"
     CDS             12476..13408
                     /locus_tag="NK767_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694698.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13562..14851
                     /locus_tag="NK767_001703"
     CDS             13562..14851
                     /locus_tag="NK767_001703"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCH6694699.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15098..16348
                     /gene="clpX"
                     /locus_tag="NK767_001704"
     CDS             15098..16348
                     /gene="clpX"
                     /locus_tag="NK767_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCH6694700.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16431..17018
                     /locus_tag="NK767_001705"
     CDS             16431..17018
                     /locus_tag="NK767_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCH6694701.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(17092..17247)
                     /locus_tag="NK767_001706"
     CDS             complement(17092..17247)
                     /locus_tag="NK767_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694702.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            17383..18711
                     /locus_tag="NK767_001707"
     CDS             17383..18711
                     /locus_tag="NK767_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694703.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            18722..>18871
                     /locus_tag="NK767_001708"
     CDS             18722..>18871
                     /locus_tag="NK767_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694704.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHK"
BASE COUNT         6166 a         3353 c         4072 g         5280 t
ORIGIN      
        1 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
       61 aagatttctt tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg
      121 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
      181 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
      241 tgtttgatct ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg
      301 ttccctcgct gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
      361 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
      421 ggacaagcta agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
      481 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
      541 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
      601 ctaaaaatta gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa
      661 aaaaatggca tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat
      721 gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt
      781 atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat
      841 tagtagatca atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag
      901 tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg
      961 cactgacaac tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca
     1021 agattgctgg ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa
     1081 atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg
     1141 ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg
     1201 cttctattga aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg
     1261 agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga
     1321 aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga
     1381 accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat
     1441 tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg
     1501 acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag
     1561 ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt
     1621 ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc
     1681 atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac
     1741 ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg
     1801 gtggtcgcta cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg
     1861 atgaattgaa aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg
     1921 atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat
     1981 tccaggccaa gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta
     2041 tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga
     2101 aaaaatctgg cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag
     2161 cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct
     2221 atgacttggt caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg
     2281 acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca
     2341 ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga
     2401 tcaatacggc aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg
     2461 tgcttccgct gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg
     2521 tagaaaaaaa catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc
     2581 cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata
     2641 tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac
     2701 ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc
     2761 ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat
     2821 gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg
     2881 aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc
     2941 gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg
     3001 caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt
     3061 gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct
     3121 tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg
     3181 aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg
     3241 cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg
     3301 aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt
     3361 atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag
     3421 tattgccaaa agtatcagaa gaactacgag aattgatgga agactggtta gtacacatgt
     3481 ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac
     3541 gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca
     3601 gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc
     3661 atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa gtctattcca
     3721 atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta
     3781 gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt
     3841 atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca tttgaagaag
     3901 cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc acacatggct
     3961 tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggttatt
     4021 ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca atgactttgg
     4081 actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt
     4141 cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag actgtccacg
     4201 acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga
     4261 aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga
     4321 cggaccttga agcagaagaa agaagagctg ctagaagagc tgccagagca gaacgacgta
     4381 aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat
     4441 aattctctga agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt
     4501 tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca tatcagcctc taaaattaaa
     4561 acatatgata tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca
     4621 aatcggggaa ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc
     4681 tctgaatttt ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact
     4741 gatccaactg ctgcgaggca caagagcgat tcagcttctg aaaggtgtag ctatttttat
     4801 tgttatccga atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca
     4861 agtcattacg tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg
     4921 actggaacat cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga
     4981 tgagaagatg atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg
     5041 tgcgttgatc actatcgaac gtcatactgg attagatgag tatattgaga cagggattgc
     5101 tttagatgca gatatcactg gggagctgct gatcaatatc tttattccta atacaccgct
     5161 gcatgacggt gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc
     5221 tctatcagaa agtatgttga tccctaaaga attcgggact cgacaccggg cagctgtcgg
     5281 tataagcgaa gtcagtgacg cgattaccat cgttgtctct gaagaaaccg gggacgtcag
     5341 catcacgtta gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatctt
     5401 gcgtagagaa ctaattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt
     5461 cctagacggt atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc
     5521 attctttaca gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc
     5581 cagtccgttc caaatacgct ttctggaaat gaagcgtata atcaaacagt tacgggggtg
     5641 ccgatcaaga tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg
     5701 actgtcaaac tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca
     5761 cggatgttcc gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaagttccg
     5821 ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca
     5881 gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg
     5941 ttcacaccat ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc
     6001 actacaggtg acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg
     6061 atggtcacag atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg
     6121 gaagtattaa gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag
     6181 ccaagcaagt cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa
     6241 agctatgatt tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt
     6301 gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca
     6361 actcgtaaaa ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt
     6421 gtggagatta cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg
     6481 acacaagaaa atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca
     6541 tcgacaacaa ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca
     6601 gaagaaacat cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat
     6661 taagttaaag gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg
     6721 gtgaagccaa caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt
     6781 atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata
     6841 caagaatctc aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta
     6901 tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc
     6961 aaaaagcaag cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa
     7021 tcaaattttt cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag
     7081 cattattaga tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg
     7141 tagaagaatt tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg
     7201 gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg
     7261 ttaatcgttt gtttgccgat cttgaaacag atttttatac aatgggcaca aatccagacg
     7321 ggttaaatat caatgacggc gtaggctcta cccatccaga acgtttagct gagatggtcg
     7381 ttgaaaaagg agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg
     7441 tggatgaact tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctaaatact
     7501 tggcagaaaa aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag
     7561 gattccacaa agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc
     7621 gttacgttgt agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc
     7681 acatgatttt cttagatttt aacacaactg gagatgggat gctttcagga attcagttac
     7741 tgaatatcat gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct
     7801 atccgcaaaa attagtgaat atccgagtaa gtgataaaaa cggtgcgatg gatgttccgg
     7861 caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag
     7921 ttcgtccttc cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga
     7981 aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt
     8041 aagagatatt ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc tgacactggt
     8101 ccttgttctt ttattattct actgtcactg atttcgctaa attacgtggt ttgtcaacgt
     8161 cgaatccccg taatagcgtt gcgtaatatg caagtaattg agtcggtacg actgtggtta
     8221 gagtagttag ataagggtgg acagttggta gcaccagctg gtcactttcc ttttctaatc
     8281 cctctgagac aataactagt gttttagcgc cacggctttc gacttctttc aggtttcctc
     8341 gagtatgcgc accagtcact tcttcactga tgattccaat cactggtgtt ccttcttcga
     8401 tcaaagcaat cgttccatgt ttcaattcgc cagcggcaaa accttctgcc tggatataac
     8461 tgatttcttt caatttcaaa gcagcttcaa gagaaacgtt gtagtcagcg ccacgtccaa
     8521 tgtaaaaggc atttcttgta tcgctgagat atacttctgc caactcttct aaataatctt
     8581 tttgggcgat gaccgattcc atcgcattag caactaaact caattcatga gtgatatcga
     8641 atacaagaga agtttcaaat tcttttttat caccgatagc tttggctaat aaagttagta
     8701 cagctatttg tgcagtataa gctttcgttg atgctactgc gatttcgggt ccagcatgca
     8761 atagcagtgt aaaggatgct tcccgcgaaa gagtagaacc agcaacattt gtaatcgtaa
     8821 gtgaagggta gccgagacgg ttgattttta ccagaacttg gcggctatct gctgtttcgc
     8881 cactttgact caagaaaata aagaatggtt tttttgaaag aagaggtgtg ttatagccaa
     8941 attcactggc taaatgaacc tcaacaggga tctgtgtcaa tttttccaga gtctgtttac
     9001 ctgctaaccc cgcatggtaa ctagttccgc aagcaacgat atagattcga tcgctatcag
     9061 acaatgtatc gaccagcttt tgatcaagag agacacgatt gtcttctgtt acgtattttt
     9121 gagcgatacg acgcatgata ataggctgtt cgtcgatttc tttcagcata taaaaaggat
     9181 aggtaccttt ctcgatatct gaagaatcta cttgtgcttc gaatgaagaa cgttctacga
     9241 tgtcacctgc aaaattttga ataataactt tttctgatgt gacggtaacc atctcttcat
     9301 cttcaatctc aacgaattcc ttcgtatgag caagcatagc cattgcatcg ctggcaatca
     9361 cattgaatcc atcgccgagt ccgattaata gtggactttt atttttagcg acgtaaataa
     9421 catcaggtgc tgtccgatca atcaaagcga aagcgtaaga acctttgatg acccgcaaag
     9481 cttttaaaaa cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt agatgagcga
     9541 taatctcagt atcggtctca ccaataaagt ggtcatttgc taaataagca attttaagtt
     9601 cttcaaagtt ttcgattacg ccattgtgaa cgagcacgaa gcgtccactt tgggaagtat
     9661 gtggatgggc gttttcttca gaaggttgcc cgtgagttgc ccagcgtgta tgaccaatac
     9721 ctatatttcc tgtagtatca atatttattt tgtttttcaa attttgaatt ctgccttgtg
     9781 ctttgaccag ataatcagtt gtgccatctg agacaaagat accagctgaa tcataaccac
     9841 ggtattctaa tttttctaga ccgtcaatca atcctggtgt tacatttttt aatccaatca
     9901 ttccaacaat tccgcacata aaaaacatcc tactttctat aatgaattcg tatgaatgct
     9961 tccagctgca ggaaaatcct tttgttttat ctttgcagat attttttgtg gattttctgt
    10021 agagtgcagt ccctagggcc atccgccgaa tctttcgata aaccctctcc tcgtcatctg
    10081 tttacacaga cctggcgcta tcctaaaagg tataactatc ctcctctttt gttttttatc
    10141 tggaaacatt taaaatcata accttttgcg aatgttcttg tcaacaaaaa aagaataaaa
    10201 aataaattgg tatatatcaa atgctttgtc aaatgctctt tttatgtcaa aaaataatgg
    10261 gtttttatct atcatctttt tcaggagttc ggtacaatag aaagtaatcg gatagaggag
    10321 gagtaaaaat ggattcaaaa attgagatca tgacactggg aatgttaaaa aaacaattgt
    10381 ccaaattcga agcatcagct ggtgtatcag atgatacgaa aatattttta gatactggct
    10441 gggatagcat ccaagaaatc gcacctgatg ccttggaagt tgctgaagct cgtgagttta
    10501 cggtagaaga tgaattgaca aaagagtcgt tttccgggta tgcgcgggaa gaaaaagcag
    10561 agcggttcga cacttctgag cagtcagaaa ctgtgatcgt cataaaaaac ttgtattaga
    10621 aaggagaaag aatgaataag agacgttggg cagcagttct gattgctgca ggattatttg
    10681 tggtttcgtt agtttcagct agtttgacta agcctcagga aaacgatcaa attgagggat
    10741 tgaacagttg gttatatggg gatgaagaac tagcttcaaa tgtgttggaa gagggagaca
    10801 gtacgcaaaa aatcgtaaaa ttatcagtag atggaacgat tgctgcggga gggtcttcag
    10861 gtttatttgc ttctgaaggt tacaatcata aaaattttat ggaacaactg aaggcgattg
    10921 aagaagatcc aacagtcaaa ggtatctttt tagaagtaaa ttctcctggt ggcggtgtct
    10981 acgaaagtgc ggaaatcgct aagaaattgg atacgattcg taaagaacat gatatcccaa
    11041 tgtatgtcag catgaaaaac atggcagcta gcggtggcta ctatatttct gctcaagcag
    11101 ataaaatctt tgcaacagaa gaaacagtga ccggctcaat tggtgtgatc atgtcaggat
    11161 tgaattattc aggacttcta gaaaaactgg gtgtggaaga tacaacagtt aaaagcggag
    11221 cgttgaaaga tatgggttca ggaacgcgac cagagacaaa agaagaagaa gcggtgcttc
    11281 aagcatatat cgacagcgca tatagtcgtt ttgtagcaat cgtcagcgag ggacggaata
    11341 aatctgaaga agaagtcaaa aaaattgctg atggaaggat ttatgatggt gtccaagcaa
    11401 aagaagtagg tttagtcgat gaattgggat ttcctgaaga tgctttaaaa gctatgcgaa
    11461 aggaacaaca gcttgaagat gcggaactag tagaatattc ttccacgcca tctgggtttg
    11521 ctagtacatg gttaggaagt aaactagcag aattccaggg attgaagcct tctgagacta
    11581 gtcagatctt atccgttctt gagaatctag ggacagcgga atcaccaaaa gcaatgtatt
    11641 attacggagg tgagtaagca tgtcagagct ttcaaatgaa aataaggaac aagaaaaaga
    11701 taagcaacag cagcctgctg taggacctga ctccaacttt tcccaaaagg tactgagctc
    11761 ttttgaaaga gagcctttga cgcccgaaga aatcaaaaag aaacaacgta aatggaaaaa
    11821 gtatacggac acaagaaatg aaccgccaaa agcaaatgat tttcctaagt atttctatgc
    11881 aggttttttt atccgaatgt ttgctttctt ggtcgatctt ttgtgtatcg gagctattac
    11941 gcgtttaaca cttggtattg ccaaaaatct aggttggctt aatttttcag attcttatct
    12001 gagtatctac ggattcacag ccttacttat ttatctagcg tattttattt tattgacgaa
    12061 attcaatcat ggtcaaacga ttgggaaaat gatctttggt attcgagtca tcagctttga
    12121 tgaagaagaa cttagctggt caaccgtttt agttagagaa ggtgcttgcc ggttcattct
    12181 aaaatttcct ttgctgatgt tagggtatct tccagcaatc tttaatcaga aaaagcaaca
    12241 tctaggcgat tttttcacta atacaagtgt tgttacgatc aatctgatca aagcattcaa
    12301 ccaggaagtc aacagctgat gagcaataaa aagcagtaac agttgaaagc accttctaaa
    12361 agtcagatct aaaatctagc ttttagaggg tgcttcagtt atttttaatt gtcatgcttt
    12421 ttttgttttg ctactattga acatgagctg aacatgagca agaggaggac actgcgtgaa
    12481 gccgattgat tttccagaaa aatatgagaa tttaatgcga gtagcacaac aagcactagc
    12541 aaaccagcaa tatcagcagg caaaagaatt atttcaacga gcttacgagt tgaaagaaag
    12601 ctttgaggct aatagcctgc ttgttttttg tctttacgag ttggacgaga aaaaagaagc
    12661 attgaagcaa gcactcttgc atgaaaaaca gtatcttcaa aatgaggagt ttgctgaatt
    12721 ttattttgac ttgttaatca gtacacaaga ttttttatat gctagaaaat tgatagcaag
    12781 tacagatttt tatgaagctt tcgagcagcg ggtcattgaa aaaatccaat ttgcagaaga
    12841 attatccgga caaatggaga ggcagaagat caaggcactc cacaataaat cggaggaact
    12901 tccttcgttg gaacctgcac gtcagcttgc gtctattgat gagatcgaac agcttcctta
    12961 tcatgagttt atccaaacag cgagtaaact tattgtatcg ccagaggtcc atattttagc
    13021 aagggcaaaa ttactggaga cacttaggca attgaacgaa tgccatcctg ttttctattt
    13081 gacgattgaa gaaaagttag tcaaagtcat accaaaagat ctgccaaagc cgcagcagca
    13141 ggcaagttac cagcaattat gtgtattttc agatcgttac ggtaatgaag atgcgctgct
    13201 tagttccgtg ttaaatgaag aattcacgtt gcaatctgca attgtttatc ctgtctatga
    13261 tacatacatc gaagacccga agcgctggtt tcaactgact gtagaggcgt gtacaggaaa
    13321 aacgatgtat gaaggtacag aagatgaaaa acaagatttt ttaaaaaaaa gagaaaaaat
    13381 tttgcagcaa atgatcttct tccattagat tttatcagtg aaaaagataa aaatgttatg
    13441 aaaaacaagg attctatcca ttcaatatgt ttacaatttg aaagggttag tgtactattt
    13501 aaaggtatgc atgaaacgtt tttgatgtat aaaatgaatt tctaaactcg gagggaatta
    13561 aatgactgca acatttgaaa aaaaaggcac gaatgatggt gttttaaaat ttacgattgc
    13621 gcaagacgaa atccaaaaag gattaacaac tgcgtttaat aaagtgaaaa aaagcttgaa
    13681 cgtacctgga ttccgtaaag ggaaagtttc tcgtcaagta ttcaaccgta tgtacggtga
    13741 agaagcttta tatgaagatg cattgaatgc tgtattacca gaagcttatg aagctgctgt
    13801 caaagaagct ggcatcgatc ctgtttctca accaaaaatc gatgttgaaa gcatggaaaa
    13861 aggtcaagac tgggtaatca ctgctgaagt aacagtgaaa ccagaagtaa aattaggaca
    13921 atataaagat ctagttgttg aaaaacaaga ccgtgaagta acagatgctg acgtggatgc
    13981 acgtatcaaa cgtgaacaag aagcacaagc tgaattagtt atcaaagaag atgctgcagc
    14041 tgaaaatggc gatacagtag taatcgactt cgaaggcttt gtagatggcg aagcgtttga
    14101 tggcggaaaa ggcgaaaatt actcacttga attaggttca ggctcattca ttccaggttt
    14161 tgaagatcaa ttagttggtc ataaatcagg tgaaagtcta gatgtaaatg taactttccc
    14221 agaagattat caagcagaag atcttgctgg aaaagaagca gtcttcaaag tgacaatcca
    14281 tgaagtaaaa gcaaaagaag tgcctgaatt agatgacgaa ttcgcaaaag atgttgatga
    14341 cgaagtatca acattagacg aattgaaaga aaaatatcgc aaagaattag tagaaacaaa
    14401 agaaaaagca gcggacgatg caaaagatga agcagcgatc cgtatggctg ttgaaaacgc
    14461 agaaatcgtt gaattgccac acgtgatggt tcatgatgaa gtacatcgtt caatggacga
    14521 attcttgaac aacatgcaac gtcaaggtat ctctcctgaa atgtactatc aattaactgg
    14581 ttctacagaa gaagacttgc ataaacaatt cgaaggtgaa gcagaaatgc gcacaagaac
    14641 aaatcttgtg atcgaagcaa ttgcagctgc tgaaaatctt caagcaacag aagaagaaat
    14701 caatgcagaa atccaagaat tatcagaaac atacaacatg ccaatcgaac aaatcaaacg
    14761 cgtgttgaca gaagatatgc tggaacatga tgtgacaatg aaaaaagcaa tcgaattgat
    14821 cacaggtaca gcgacagaag cgaaagaata gaaatcaagt aataaatcat ttctgaagaa
    14881 gctacttcta gaaaattgtt tattcgaaat aagggactgt gacacgattt tgtcacagtt
    14941 ccttattttt tgttaagagt aaaaaatgca aaggtatagg tcagttgatc ctttgttaaa
    15001 cactttttct ttttactaaa ctatgttatg atagtaaaag actgtaaagt ctttgatagt
    15061 ttcttgcata ctttttgcaa gttaaggggt gaagaccatg tatgacaatc caagtagcaa
    15121 tgagaccgta cgctgttctt tttgcgggaa aacccaagaa gaagtgaaaa agatcgtagc
    15181 tggtccaggg gtctacatct gtaatgaatg tatcgaccta tgtaaagaaa tcattgatga
    15241 agaattttat gaagaagctg tacgcgaatt cactgaagtc cctaaaccac tagaaattct
    15301 tgaagtattg aataactacg tgattggaca agatcgcgca aaacgctcgt tagctgttgc
    15361 cgtatacaat cactataaac gtgtcaatca gcaagcacaa gaaaacacag atgatgttga
    15421 actgcaaaaa agcaatatct gcttgatcgg acctacaggg tctggtaaaa catttttagc
    15481 acaaacatta gcaaaaacat tgaatgtacc ttttgcaatc gcagatgcga caagtttgac
    15541 agaagctggt tatgtggggg aagatgtaga aaacattttg cttaaattgc ttcaatctgc
    15601 tgattacaat gtggaacgtg cagaaaaagg gatcatctat attgatgaga tcgataaaat
    15661 tgcgcgtaaa agcgaaaatg tatctattac tcgtgacgtt tccggcgaag gtgtccaaca
    15721 agccttgctg aaaattctag aaggaactgt tgcaagcgtg ccacctcagg gaggacgtaa
    15781 acatccacat caagaattta ttcagattga tacgacgaac atattgttca tcgtaggtgg
    15841 tgcatttgat ggcatcgaaa cgattgtgaa gaatcgtatg ggcgaaaaga cgattggatt
    15901 tggtacacaa aaccaaaaac tttctgaaga cgaaagtgtc atgcagcata tcattcctga
    15961 agatttacta aaattcggat tgatccctga gtttatcgga cgattacctg ttatggcagc
    16021 tttagagaaa ttgacaacag atgatctggt acggatcttg actgagccga aaaatgcgct
    16081 agtcaaacaa tatcaaaaac ttctttctct agataatacc gaacttgaat tcgaacctga
    16141 agcgttgaga gccattgcga aaaaagcaat cgaacgcaat accggtgctc gaggacttcg
    16201 ttccatcatt gaagaaatca tgatggatgt gatgtttgat atcccttcaa atgaaaacat
    16261 cgaaaaagtg attatcacaa aagaagcagc tgaattgtct ggtgagccaa cagtaatcta
    16321 taaagattca gagaaaaaag cgggttgatg aaaagaggct gccgtaaaag tcattttggc
    16381 ttttgcgaca gccgcttgct ttattcatca ccttgttaag gagagagacc atgaaagtcc
    16441 atcaagctga aattatcata agtgcagtgg caccaaaaca atatccagat acacaattgc
    16501 cagaaatagc tctggcagga cgatcaaatg tagggaaatc ttcttttatc aacacattga
    16561 ttgatcgaaa gaatttagcc cgtacttccg gtaagccagg aaagacccag acattgaatt
    16621 tttatttgat cgagaacgca ttacattttg tcgatgttcc gggatatggg tatgcaaaag
    16681 tatccaaaac tgagcgagca aaatggggaa agatgatcga gacatattta acgactagag
    16741 accagctacg tgcggtcgtt tctttagtag atttgcgtca taagccttct caagaagata
    16801 tccagatgta tcaatttttg aagtattatg atatccctgt aattgttgtt gccactaagg
    16861 cagataagat ccctcgtggt aaatggaaca agcatgagag cgaaatcaaa aaagcgttgg
    16921 attttgatcg caatgacacg tttattctct tttcttccgt tacaaaacag ggaaaagaag
    16981 aagcgtgggc agcaatcgaa caagcaattg aatcataaaa agaataaaaa agacttacca
    17041 gtcaaactct ttcataaaag tttggctggt aagtcttttt ttgagcttaa cttacttttg
    17101 tttcttgtct tttttctttt cattttgtgg ctgtggtttt agaaatcgat cgatctcttc
    17161 ttttggtgca actgtttttt ccggatcgac agcaaatgat tcgaaaagtt tatctaaatt
    17221 atctgtacgt tcaaatttca accccattgt aaacacctct ttgtttcttt ttacttagtc
    17281 tatcatgtaa tcgtccaaat tggtttctaa gacataaagt tttagttttt ctttatcgtt
    17341 ggttataatt aaattataaa aagattttaa agaggtgaga aaatgattgg cgaagataaa
    17401 ttattgatga ctatccagct cacagactta aatgaagaag aaaaaaaaca ggaaataagc
    17461 gattgggcta ttttgacacg tctgttgatc cagccaacat ttatccgctc tttcacacaa
    17521 caagcggatc cgtatgatct tcgaaagctt caagaaaaaa taatgctagc atcggtaaga
    17581 gacgaaagtt ggttagttgt aggaaatgac gagaacgaat gtagttttcg cttggaagat
    17641 aatcaattat tgataaaaaa tgtattgtct atctctgttt tcaaagaaaa gcaatttttg
    17701 attcgagact tcattcagaa aaaaatgatt gctcatgggg tgttcgccta tatgcgagct
    17761 tatagcgagt ttatctatca caatacaaaa cagatctctc aacggctatt gtttgagaaa
    17821 aaggatgaaa tcgaacattt acctaaaatg aaacaacaaa acggggaagt agtagtcgat
    17881 tgcaaccaat ttccaggata cgatcttttt taccaaggtt tatgtttcac ttcgtgttgg
    17941 gaaatgtatt atagtcgcta ttatcatcaa atcatcccta aaccgatttt tctcgacatc
    18001 caacaagtag aaaaagtgaa ggaattagaa aacgaagtca tatgtatcca gctttatcga
    18061 gaccccttta actggcaaaa gccaaacaat caaatgtttc aatcctattt tagggaccag
    18121 cttgggtttg atcatcttgc ttgggacaat ggtgtcggtt tattgaaacc gccattcgtt
    18181 gaatacattt acactgacca tacgatccaa tctgtccagt atcagaatgc acaaatgcaa
    18241 cctgttccta aaaagaatgc ttcatttttt gtaacaaaaa gttatgatat tcaacaagga
    18301 gattataaag aaaggcgtgt tcgcggaaca ctaaatgctc aggcgtattt tccttgggtt
    18361 gacgagaatc gctcgcgcat gatgtgctat aaagttatcg atccgactgt tgctttggat
    18421 aatggaatcg aagcttattg ttattatatt agggaatatc tagaagtaga ggtaacagat
    18481 gaaaaatatc aagagtatct tttatcctta agaatctatg ttccttcaga atccctgtca
    18541 gaattaccgt taaaggaaat caaacataga ctctctgatg ttaccttcaa acgaataaag
    18601 aaaaagcggc gaagtatcca attcgatgtc aagaaaggtg agcagcatct gcgtgtgcaa
    18661 tttttagatt accgagaatt agaacaattg attactttac aaaaaatatg aggtgaagaa
    18721 aatggataaa caactctatt atcgaataat ggttccggca tcattattat ccgtctgtct
    18781 cttttttgtt cctaattttc ctattcgatc agtcatcggt tttttggtca tgtttggcgg
    18841 gatgggactt tattattatc ttcttcataa g
//
LOCUS       DAHUVV010000050        17929 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.051, whole genome shotgun sequence.
ACCESSION   DAHUVV010000050 DAHUVV010000000
VERSION     DAHUVV010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.051"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(23..1606)
                     /locus_tag="NK767_001709"
     CDS             complement(23..1606)
                     /locus_tag="NK767_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCH6694705.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(2184..2327)
                     /locus_tag="NK767_001710"
     CDS             complement(2184..2327)
                     /locus_tag="NK767_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCH6694706.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2549..3028)
                     /locus_tag="NK767_001711"
     CDS             complement(2549..3028)
                     /locus_tag="NK767_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCH6694707.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3101..3385)
                     /locus_tag="NK767_001712"
     CDS             complement(3101..3385)
                     /locus_tag="NK767_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694708.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3463..3657)
                     /gene="ssrS"
                     /locus_tag="NK767_001713"
     ncRNA           complement(3463..3657)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="NK767_001713"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3729..5015)
                     /locus_tag="NK767_001714"
     CDS             complement(3729..5015)
                     /locus_tag="NK767_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCH6694709.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5153..5824
                     /locus_tag="NK767_001715"
     CDS             5153..5824
                     /locus_tag="NK767_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCH6694710.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            5790..6278
                     /locus_tag="NK767_001716"
     CDS             5790..6278
                     /locus_tag="NK767_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HCH6694711.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6291..7238
                     /gene="prmA"
                     /locus_tag="NK767_001717"
     CDS             6291..7238
                     /gene="prmA"
                     /locus_tag="NK767_001717"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCH6694712.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7239..8003
                     /locus_tag="NK767_001718"
     CDS             7239..8003
                     /locus_tag="NK767_001718"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCH6694713.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8236..10449
                     /locus_tag="NK767_001719"
     CDS             8236..10449
                     /locus_tag="NK767_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCH6694714.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10460..10906
                     /locus_tag="NK767_001720"
     CDS             10460..10906
                     /locus_tag="NK767_001720"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCH6694715.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11077..12336
                     /locus_tag="NK767_001721"
     CDS             11077..12336
                     /locus_tag="NK767_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HCH6694716.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12425..13372)
                     /locus_tag="NK767_001722"
     CDS             complement(12425..13372)
                     /locus_tag="NK767_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCH6694717.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            13766..15058
                     /locus_tag="NK767_001723"
     CDS             13766..15058
                     /locus_tag="NK767_001723"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCH6694718.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15073..16842
                     /gene="aspS"
                     /locus_tag="NK767_001724"
     CDS             15073..16842
                     /gene="aspS"
                     /locus_tag="NK767_001724"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCH6694719.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            16951..17826
                     /locus_tag="NK767_001725"
     CDS             16951..17826
                     /locus_tag="NK767_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCH6694720.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
BASE COUNT         5659 a         3341 c         3837 g         5092 t
ORIGIN      
        1 ttttttgttg cgaaaaaacg tcctagtcaa tcagttggcc gagcggctgg cggctgatct
       61 gctcctgatc aataacgagt gcttcttgga agtgttgctg caaaaagtag cggtcatggc
      121 tgactgctag gagcgttcct tgatagtctt gcagcatttc ttcaatttct tctcgagcat
      181 agctgtcgag gtgattggtt ggttcatcca aaatcaggac gttgattttt tctaaaaaga
      241 gtttcagcaa cgccaaacgg attttttcac caccggataa atctttgatc cgtttaccca
      301 catcgctttg ataaaagcca cttttggcta attgccgtcg agcgacttct tcgtttccag
      361 tcaatgtgat ggcgtaagcc agcaaacggc tatcgggatc ggcaaactgc agctgctgcg
      421 gcaaataacc gatttgcaag ctactaccta atttgatggt gccaccgtaa ggcttttcct
      481 caccgagtaa taatttgagt aaggttgatt ttcctgcacc attgtcgccg atgatggcta
      541 aatgatctcc acgatagact gcaaaactgc tgtctttgaa tagtacttga tcagcgtact
      601 gctggtgcaa gttttgaatg atgaaaactt ctttgccaga ttttccacca ttagacaagc
      661 tttgcagccg atttttcggg ggaggtggcg gtttgacgag tgtcagtttc gccaggcggc
      721 gttccagttc tttggctttt ttgaagaagg actcattgtc tccttcatgt gcccactgac
      781 gaaaacgacg aatcatgact ttcaaccgct ggacttcttt ttgctgcagt tcgtaatttt
      841 ttgtcagctc agccaagcga gcttctttca agactcgaaa acggctgtag tttccgggat
      901 actcaatcaa tgcaccttct tccagttccc atgtttttgt ggtgacttga tcgagaaatt
      961 gccgatcatg agaaacgacc agaaccgatt tttttgtatt ctgtaaatag ttttccaacc
     1021 agcgaatact gttaagatct aaatgattgg tcggttcatc gagtaaaagt aaatcggcat
     1081 cggctgctaa aattttggct aaagcgactt taactcgttc gcctccgcta agttctgtta
     1141 accgtgcttc ctgacggtgt ggaatcccta agccttgcaa gatcgctgca atccggtctt
     1201 ctaatacgta accgcctagt tcttcaaagc gatgctgcag ctcaccgtaa cgggccagac
     1261 acttctctaa gtttgtatct ggttctgtca tcaatgcttc gtagcgactc atttgctgtt
     1321 tgaggtcctg tatctcagcg gcagaatgga gcaagtagtg gttgacggtc gcttcggaaa
     1381 ctgtaagttc ttgttcgacc gtcccgatct ttaagccttt tttgcgactg ataacaccgc
     1441 tgtcgacacc ttctctgccg gtcaagattt gtaaaaaggt tgatttgcca gttccgttga
     1501 taccgactaa ggcaattttt tcaccagctt caatttgaca ggataaatcc tcaaataatg
     1561 gattcccgcc gattgttttt ttaatagaaa ttgcttgtat gatcatgatc tctctccttg
     1621 agaccactca aaaaggccat ggttatccct ggcctgtgtt atttaaactc agattatcat
     1681 gagaaaaata ccttgagatg gccattgatt ttacgtgaat tcaactgttt tggacaaact
     1741 tgtcccgtca acagcaactg taaaaccaat tgcatggcta aatgcgatat ggtatcgttg
     1801 gtaattttca ttacttgtta ttttagtcat gcgcatcata gtattttccc tccttcgtgt
     1861 agttgtcttt tccagtgtac ctgaaagtgt cagaaagttc aactagcgct gacatttact
     1921 taatatttat cgatgtgtaa ttctgtcaga gagctttttt tatctgactg agggtaatgt
     1981 ctcgtggacc acatcattgt attctgagct aaaccagcgt tcaggcgaaa tcatatggtg
     2041 tttgataaaa aactttgtct acatgacttc cttttgccat ttgcttaata ccgttgcatt
     2101 aaacagctct tctctttgga aatctcacca acatcgcgtt caggagtatc gttgtggttg
     2161 caatggcaag cggtagcctt tcatcaattc ttgaatgatt taccgaatgt agcacttgcc
     2221 ttctcgcggt acccgaaccc gaaataaaat tttagtttgg cgataaaatc ggtatccgcc
     2281 atcgatactt catatttttt cgtatcagat tcatcggaaa cttccacatt gacgaaccaa
     2341 taagctttgg catgcttagg tcgttttaaa ccacaccagg gcgactaatc gccctggtgt
     2401 tcactttttt tattttactt gaactcattg actgttttcc ctttatttta aacattataa
     2461 agaccgatga gtgcttgccg cgtcttttgt ttttatcacg gttttttgtg caaaaaaacc
     2521 tgccgatagt tggcaggtcg tctacgtgtt aagctttggt gtggtcttta gctggcaatt
     2581 ggttttctaa atcagttcgg acaacaagca catcgcaagc tgcgtttcta ataacatatt
     2641 cagaaacaga accaataaat aaacgttcta cagcattcaa tcctgtggct cctaacataa
     2701 tcaaatctac ttgattatct tctgggattt ctctggcaat aatttgtttt ggtgagccat
     2761 attctacgac agaggttaca ttattcaagc cagctttctt cgcattgctt tcataatctg
     2821 ccaatgtttg ctttgccatt tcagtagctt gttctgcaag cattccatca aaggatgaaa
     2881 cggtttggaa agaacgtgtg tcaatgacat gtgctaatag aagttctccg ttatttcgga
     2941 tagctacatt gaccgccttt ttaaaagcta attcagcttc atcagaacca tctacggcaa
     3001 ccatgatttt tttgtattgt tgtaacataa gcattcactc ctcaagtcaa tcgtattgta
     3061 ctcttttatt gtagaacact ttagggaaaa agagaattat ttactgcttt ggttcagcaa
     3121 ttttttttgc aaatcggata ctgaaggtgg cagaaacaat aattacgatc agtaaaaaga
     3181 ccaaggcaat aaagcgttga ttgaaaaaag ctgttaaaat acccattacg cctaaaacag
     3241 catatgcagc accgtaaaat tgtaggaatc cttgattttt atcatttttg ttgatcaata
     3301 caaaaaagat atcctgttta ttaaataaat aatagctaac gaataataat actaaagtgg
     3361 cgataatcat taaaatccta atcatgaaca aatcctccaa aataagcaac aaatagaaaa
     3421 agcaatttgc caagagcaaa ttgctttatt ctttaaaaga tggtaatctc cgttaatgcc
     3481 gttgtttgtc ctgtagtatt gatcccgcga attcaagcag gtgggtccca tggtcgtagc
     3541 ttcgaactac caaatgataa ggcatgttac cagcagtgac acaagacggg tcccaagata
     3601 tcaataaaaa tgttcggtca acacacaaag aggacaattc gaacatcaca gatttcccac
     3661 ctctatagta gcatatgaat ttaaaaagag caatgagaga gctcgcagaa acagaaccgt
     3721 tttcttgtct agttttttgg aggatgcttt ttccactctt ttatacggta atactgttga
     3781 cccagagcct gttcatattt ccctgtatct tttggctgat aatactgagc attcttcagt
     3841 ttatctggta aatattgctg attgacccaa gcctgatcaa aatgatgagg atactgataa
     3901 cccacgccac ggttgagtga tttcgcacct ttgtaatggc tatctctcaa atgatcagga
     3961 acatctccag ctttgccctc tcgaatatca gcaagtgctg catctaatgc catgtaggca
     4021 gaattggatt ttggcgacaa gcaaagatcg acgacgacgt ctgctagtgg gatacgtgcc
     4081 tcaggcaatc ccaatttttc agcagctaat acagcgttca ctgtacgagc cgcagcagca
     4141 ggattaccta aacctatatc ttcataaccg ataaccatca gacgccggca aatacttgcc
     4201 agatcacctg cctcaactaa acgggcgaga taatgaagtg cagcatccac atcacttcca
     4261 cgaatcgatt tttgaaaagc cgaaataacg tcataatgag catctccatt tttgtcatgg
     4321 gtaagagctt ttctttggac acattcttca atgatcgaca gtgtaagatg aatccttcct
     4381 tctttatctg aaggagtcga tagtgtagcc agttccagcc cattcaatgc actccgcaaa
     4441 tcgccgttcg ttgcacgcga aaggtgaaga agagcatctt cgtctaactg gattgcttgt
     4501 tgtccaaggc cgcgttcttt atcccttaaa gcatgttcga ctgccaactg gatgtcttgt
     4561 tctgttaatg gtttgacttc aaagatctgt gtacggctgc ggatcgctgg attgatggta
     4621 atataaggat tctcagtagt cgctccgatc aagatgattc ggccactttc taaatgagga
     4681 agcagaaaat cctgttttgt tttatctaat cggtgtactt catccaataa gaggatcact
     4741 gtaccactca tttttgcttc ttccgctaca acttgtagat ccttttttga atctgtagcg
     4801 gcgtttagca tgcgaaatgc ataattagta gagccggcga tcgcacttgc gatactcgtt
     4861 tttccagtac caggaggtcc atatagaatc atggaagaga gcatgcgagc atcgaccatg
     4921 cggcgtatga tttttccttc cccgactaga tgctgctgac caacgacttc atcgatcgtt
     4981 cgaggtctca tgcgataagc caatggctgt tgcataggat tttcctcctt cttgttttta
     5041 cgtcccttaa ttatatcata tttagcaaga tgcctagaaa gaacagcctt ctgttgtttt
     5101 gttttcatag cttatccaac taatattgtg tatgataaag taggtgagaa aaatgacgga
     5161 taaacaaaag atagatgaat ttttttggaa taaaacgacg ccagaagtgg cacgggattt
     5221 attggggatg tatttggagc atgacacacc agaaggaaga ttagctggat atattgtaga
     5281 cgcggaagca tacctaggtc cagaagacga ggccgcccat agctatggtt taagaagaac
     5341 accgcgtgtg cgggcaatgt atgaaaaacc aggtacgatc tacctttaca cgatgcatac
     5401 tcatcggatt ttgaacatca ttactcagcc agaagggatt cctcaaggag taatgatacg
     5461 tgcaatcgaa cccgccgcaa tgattgatca aatgagtaaa aatcgtggcg gaaaaactgg
     5521 tccagatatc agcaacggtc cgggaaaatt agtggaagca ttagcaattc ctcaagaact
     5581 ttatggtcag tctatagcag atagttcgtt acgcttagtt tttgagaaaa aaaagacacc
     5641 aaaaaagatc aatgctttgc ctagaatcgg tatcccaaac aaaggtgtat ggacagaaaa
     5701 gccgttgcgt tttgttgtgt cgggaaatcc ttatatctct ttacaaagaa aaaaccaaat
     5761 cgagaaagat tggggttgga ggaaagaaaa tgaaaaagaa ggatctatta acatatttag
     5821 atgaaataat tgaaaaacaa gtgacagact atgatgtagc tttagattgg gatacaaaaa
     5881 atcatacgat cgagatcgtt gttcgcttgt ttgcagaaaa taaagcacaa tttgagattg
     5941 atgatgcaga agggatcgtt tcgcaagaac caattatcga atttgaagac ggcgtacttc
     6001 tttttaatcc tcagaaatct gtttttgatg aacgcaatta tctagcagta atcccttatg
     6061 aaggaaaaaa agggttggca aaatcagtag ctgattcttt agtggaatat ctaaatgaag
     6121 tattagctca agggcaaagc gatttgcttg attttttgga tgaagacgat gacgaagctg
     6181 tattcgagct tcactgggat aatcaacaac tagcagaatt agtagaagta aaacagcaaa
     6241 atgaagcaga tacttattta ccgtacccaa gttattaagg agtgacagat atgaaatgga
     6301 atgaagtaaa agtagaaact gaaagtgaag cagtcgaagc agttgctaat attttgatgg
     6361 aggcaggggc gagtggtgtc gcgattgaag attctttaga tgttgaacat tttcagtcgg
     6421 atcaatatgg tgagatttta gataaaaatg attttacatc gctgaaagaa ggcgcactag
     6481 tcatggctta ttttccggaa acgactttct taccggaaat cttgcctttt atcaaagatc
     6541 gtatcacaca attacctgag tttggtctag ctattggaaa aaataaagta acagttagtg
     6601 aagtagagga aagcaactgg gcaacagcct ggaagaaata ctatcatccg gtgcgtatta
     6661 cgcgaatgct gacggttgcg ccaagctggg aaaactacca gacagaagat cctctagaaa
     6721 aaattatcac actagatcct ggtatggcat ttggcacagg cactcatccc acgacttcct
     6781 tgacattgac agcactagaa acagtcttgc gtggtggtga aacattgcta gatgtgggaa
     6841 ctgggtcggg cgtgctaagt attgcagcaa aatatcttgg tgcaaaagat gtctatgcct
     6901 ttgatttaga cgaagtagcc gttcgttcgg cgaaagaaaa tatggatatg aatgaaatag
     6961 ccaaagatgt ccatgtatca gctaacgatc ttttgacagg aatcgagatt gaaagtgatg
     7021 taatcgttgc gaacatttta gcagatatca tcttattgat gatcccagat gcttggcgtt
     7081 tactgaagca aacaggcacg ctgatcgttt caggaatcat tgaagagaag aagcaaatgg
     7141 ttctggatgc catgttagaa caagggttta tcgtggatca aattttccag caaaaagact
     7201 ggtatgcaat tattttgaaa aaaccagagg tggaatagat gcagcggtat tttttaaaag
     7261 aatcatatga atcaaaagac tattttgttt tagaagggga aaactatcac cacattgtac
     7321 gggttatgag gatggaaccc gaggatgaag tttttttggc attcaatgat caaatagcca
     7381 ttgttgcaga aatcacggag attactgaag aggctgttta tctgaaagaa attaaaaaag
     7441 aagaaaatca aaaggaactg ccaagttcga ttaccatagc gagtggctat cctaaagggg
     7501 aaaagctgga ttggatcgtt cagaaatcca ctgaattagg agcgcaagct tttcttggtt
     7561 tcccggcatc agcctctgtc gttaaatggg atgcaaaaaa acgtatgaaa cgacaacaac
     7621 ggcttgaaaa gattgctcaa gaggcagcgg aacaatccca tcggcagatc gtgccatcag
     7681 tagagttatt ggaaacgaaa caagatttat ttgagcggct agatgattat gacgtggtct
     7741 tggttgctta tgaggaatca gcaaagcaag gcgaaaaatc aaatgcagcc aaaattctta
     7801 gtgaaacaaa accagatagt cgaatcttag ctgtctttgg accagaaggt ggattatccc
     7861 gcgatgaagt cgattatttt gtagaacaca aagggatact ttgtggatta ggaccaagaa
     7921 tccttcgtac agaaacagca cctctttatt tattaagtgc tgctagctat caatgggaat
     7981 taatgaatcc ttctttcagc taacgttctt cattaataaa agagtatcga cagaaagaat
     8041 tgaaaaaata gtcaacaatt tgtataatag aaacaattat ttatacaatg gatacgaaaa
     8101 atcgtttgaa aaagctttta aaaagcgaac atttccgtaa aaaaacgtat aactgcacgg
     8161 attttgggac aattatcaaa cagatatttg tcccgattcc cgttttaccg aaaaaagaaa
     8221 gggggatcga ttcctgtgac aaaagaagaa attttaacgg gacccggtgt cataaagctc
     8281 gttagtcaat acatgggtcc cgaacatgta gcatttgttg agaaagcatg cgaatacgcg
     8341 actgctgcac atgacggaca gtttagaaaa tcaggcgaac cctacattat tcatcctatc
     8401 caagtcgcag gtatattagc agatttaaaa atggatcccc atacagtggc tacaggcttc
     8461 ttacatgatg ttgttgaaga tacagaaatc actttagaag atctgagaga agaatttggc
     8521 gatgacgttg ctatgttagt ggacggcgta accaaattag ggaaaataaa atataaatcc
     8581 cacgaagagc agctagcaga aaaccaccga aagatgctgc tagcaatggc tcaagattta
     8641 cgagttatca tggtcaaatt agctgataga ctacataaca tgcgtacgtt gaagcacctg
     8701 cgagaagata agcaaagaag gatcgctcag gaaactttag aaatctatgc gcctcttgca
     8761 catcgtctag ggatcagccg gatcaaatgg gaattagaag atacagcact tcgttatcta
     8821 aatccaaaac agtattaccg tatcgtccac ttgatgcaga cgaagagaga agaacgtgaa
     8881 aaatacgtaa gtggtactgt tgaagatatt cgaatagcga cagaagagct ggggattttt
     8941 gcagaaatct atggacggcc aaaacacatt tattcaatct atcgtaaaat gaaagatcag
     9001 aaaaagcagt tcaacgaaat ttatgacttg ctagctattc gagtgatcgt agattcaatc
     9061 aaggattgtt atgctgtatt aggagcaatc cacacaaaat ggaaaccaat gcccggcaga
     9121 tttaaggatt atattgccat gccaaaagct aatatgtacc aatctttgca tactaccgtg
     9181 atcggtccgg caggaaatcc ggtagaaatt caaatccgaa cacaggaaat gcatgaaatc
     9241 gctgaattcg gggttgctgc acactgggcc tataaagaag gaaaaaatga aaaagtagaa
     9301 ccagatggta tgacgaaaca attaagctgg ttccatgaga tactcgaact tcaagacgaa
     9361 agctatgatg cttctgaatt tatggaaggc gtaaaaggag atatctttag tgataaagtc
     9421 tacgtcttca caccaaaagg agacgttact gagttaccaa aaggatccgg accattggac
     9481 tttgcataca gtatccatac agatatcggt aacaaaacca ctggtgcaaa agtaaatggc
     9541 aaaatggtgc agcttgatta caaattgaaa aacggagata tcattgagat catgacttct
     9601 ccaaattcat ttggcccaag tcgcgactgg ttgaaattag ttgctactag caaagcaaga
     9661 aataagatca aacgtttctt caaagcccaa gatcgagaag aaaatgtgat caaaggccac
     9721 gaatccgtgg tcaaatgtat tacagatcta ggatttacgc ctaaagatat tttgacgaag
     9781 aacaaactgc aagaagcact cgatcgtttt aattatcaaa cagaagatga tctctatgca
     9841 gctgtagggt atggagaagt tagccccttg acgatggcca atcgtctgac tgaaaaagaa
     9901 cgtaaagaac aaaaaatcga gcagcaaaag caagaagcag aagaaatcat gaatcagccg
     9961 aaaaaagaac ctgacaaaat gaaagtacgt catgaaggtg gcgttgtcat tcaaggggta
    10021 gaaaacttac taattcgtat cagtcgctgc tgtaatccga ttcctggtga tgatatcgtt
    10081 ggttatatca ctaaaggcag agggatatcc attcatcgtc gagattgtcc gaatgttcag
    10141 cctgacaaac caaatgtagc agaacgtttg attgaagtcg aatgggaaga tacatcgaat
    10201 acacgaaaag agtatgatgc agatttggaa atttacggct ataatcgttc aggcttattg
    10261 aatgatgtac ttcaaacagt caatgcgcta acgaaaaatc tcaacagcgt tgaagcacgg
    10321 acgaataaag ataaaatggc gacgatccat ttgacggttg gtatccagaa tttatcccac
    10381 ctaaagagta tcgtggataa aatcaaagca gtacctgatg tctacagtgt acgccggacg
    10441 aatggatagg aggacagata tgagagcagt tgttcaacgg gtaagtaaag ctgctgtgtc
    10501 agtcgatcag caagaaattg gaaaaattgg ctacggccta cttgttttat taggcattca
    10561 tgaaaccgat acgcaaaaag atgtggacta tctggtaaaa aaaattgcac aaatgcgtat
    10621 ttttgaagat gaacaaggaa aaatgaatat tagtgtagaa gatgtgggag gacaaatttt
    10681 aagtatctct caattcacac tgtttgcgga tacgaaaaaa ggaaatcgac caagctttgt
    10741 ggaagccgca cgaccagaaa cagctattcc tttatacgaa tcattcaatg aaggaatcag
    10801 aagtcgcggc atcacagtcg ctaccggaca attcggagca gatatgtcgg tttctctcgt
    10861 caatgatgga cctgtaacga tcattattga ttctaaaaac aaataaagta atctggtttt
    10921 cactagtaaa aactatgaaa cacgtcgctt aagttagact gataaactct aacttaagtg
    10981 gcttttttgt ctgcaaaaag cgcttctttt gtcagcagtc ggaaaaaaag ttaagcttaa
    11041 attatacaag ttatttttta taggaggttg ctgaagatga aagaagtacc agtagtagat
    11101 atcaaaatca cagatttaaa aaaattgtat caaaaaggat acaacatgtt ggaggagtta
    11161 cgccatgaag cggacgctcc cgtcgtcaaa gcaaagattt tcaataaaga agctatcacg
    11221 atctacggat catcagcagc aaaagtattt tatgatccaa ggaatttcaa acgaaaaggt
    11281 gctatgccaa aattagtatt gaagacactt tttggtcaag gtggagtaca gactttggat
    11341 ggagctgctc atcaccatcg gaaaaatatt tttatggatc taatgacacc ggaaagaatg
    11401 gaagattatc atcgtatctt agataagaat ctgacacaag cacttgaagc acagcatggt
    11461 caatttgaac tatttgattt aagtaagatg gttttcttta catctatttg cgagtgggca
    11521 ggtatcaatt tatcagcgat ttctaaagat gaggtcgaaa aattagcaga atatcaaatc
    11581 tctatgatca gcggaacgtt tacttctccg attgatcaca taaaaggtgt cgagaatcgc
    11641 aaaaaatcag aaaaatgggc gcaaggcttg attgaagaag ctcgacaaaa tcctgtggct
    11701 ggaaaagaaa atgtggcatt atatgctttt gccaatgcca cagatttaga tggtcagctt
    11761 ttacctctgg aagtggcagc tgttgaatta ttgaacatca tccgtccaac ggttgctcta
    11821 actgtgtggg cggcattgat gggacatgca cttttcagta gaccagattt gtatcagcaa
    11881 ttaaaaaatg acttttcgac gttacaagat ccgtttattc aagaaatgag acgctattat
    11941 cctttcttcc caatgctacc tgcgatttcg ctgaaagaag tagaagtaga tggttatcgt
    12001 ataccagaag gcagttgggt tattttagat ttatatggaa cagaccatga tgaacgaaca
    12061 gttgaagcgc cagattcctt tatgatcaaa cggtatgtag ggaaagcaaa agatatttcc
    12121 tataaagaag aatatgagat gattgcacaa ggcgggggaa attttcgaca aatgcatcgc
    12181 tgtgcaggag aatggatcac tcttcatagt ctacgtgtgt tttccgatca gctagtaaat
    12241 aagtttgagt tctctgtacc tgaacaagat tggacgattc cattcaatca gtttccgact
    12301 tatccgaata gccgagcgct actttacaag aattagatta gcataaactt atcgaaaaaa
    12361 aggaagatca aacagtgctg atcttccttt tttcatatta ctataaattg ataaggtcaa
    12421 ttgatcaaaa tccacgaaat gcttcatcaa tggtttgata atcttcattg gataaatgca
    12481 aatctaaagc ttggacattg tcagatactt gctctggacg cttagctcca ggtataacta
    12541 cagacactcg tggattcttc atgtaccaag ctaaaacaag ttgcgcagga gtggcttggt
    12601 atcttttagc aatggggcga agtacatcga cagcggttaa aatttctctg aaacgttctc
    12661 cttggaaatc tggatttctt tttcgaggat ctccttcacc aaattgtttt tcctcaccaa
    12721 gttcatattt tcctgtcagc aaaccggaag caagaggaaa atagggaaca aaactgattt
    12781 tgtttttttc aagataaggg aataattctt tttcagcctg ccgatggatc aaactgtatt
    12841 tatcttctac tacatcgaca tatccatctg cattcgcttc tttcaattgt tctaacgtga
    12901 agtttgacac accgactgct cggatttttc ctgcttcttt taattcgtgc aatgtagcaa
    12961 ctgattcatt ttttggtgtg ctttcatccg gaaagtggat ataaaaaatg tcgatgtaat
    13021 cagtttgcag acgttttagt gcatcttcca ccgattgttt gagaaactca ggagaattat
    13081 caaatgttcg ccctttattt ggtacatgag cagcttttgt agcgatgacg acacgtgagc
    13141 gatcatattc tttttccttc agtacctcgc caatcagttc ttcggaacgg ccgtttccgt
    13201 aagcataagc ggtatcgatc aaattgatcc cgctattcaa agctgtacga actacttgtt
    13261 ttcctgtctc atcttccaaa ccgtcaaaaa gattatgtcc tccaacggca tttgctccta
    13321 atccaagctg ttctgcgtag acttgtgaat gtccgattct tatttctgcc atagctgatt
    13381 cctccttttt gtctagtcta aaacaaaata ccaaaaagct gaactaaaac gtttttgtga
    13441 ggggaaatag tcagatatct cttgactttg agactggttt ttagtaggtt ataagtaata
    13501 tcgaatgtca atgaaagagc gagtaagttt ttgaaacttt acaagagagg atcatcgagg
    13561 ctgaaaatga tctaaagttt cattagccta aagacactct ggagatggac gagtgaaaag
    13621 ccgtcccgcg tacgagcgtt attcgtaaga ggaaagagaa atctttcaaa attaggtggt
    13681 accgtgtatg aatttcattc acccttgttc atgatgaaca agggtgattt ttttatttac
    13741 atagctaaga aagggagaga aaactatgag tttccaaaga ccaaaaggaa caaatgatat
    13801 tttgccaggc gaatcagaaa aatggcagtt tgtcgaagaa acagcacgtt tattattcag
    13861 agattatcag tataatgaaa tgcgtacacc gatttttgaa cactatgaag tgatatcccg
    13921 aagtgtaggg gatacaacgg atatcgtttc aaaagagatg tatgattttt atgacaaagg
    13981 ggaacgccat gtgacgcttc gaccagaagg tacagcacca atcgttcgtt cttttgttga
    14041 gcataaattg ttcggcccag agtatgcgaa accgttcaaa actttttata tgggaccaat
    14101 gtttcgctac gaacgccctc aaaaaggacg gttacgtcaa ttccatcaaa tcggagtaga
    14161 agcatttgga tcagaaaacc ctgcaactga tgtggagagc atggtgatgg cacttgattt
    14221 cttcaaacag ttggggatca cgcaaatccg gctagtcatc aattcattag gtgataaaga
    14281 aacgcgtcaa gcctatcgtc aagcattgat cgactatctt ttaccttttg aatcagagct
    14341 gagtgaagac tctaaacgcc gccttcatga aaatccatta cgcgtattgg acagtaaaga
    14401 caaacgagat caaaaatttg tggcagaagc tccatctatc ttagattatc taagcgagcc
    14461 tgctcaaaaa cattttgata ccgttgtttc catgctggat gcattggctg ttccttatga
    14521 aatcgacagt aatatggttc gcggactaga ttattacaca catacgattt ttgaaatcat
    14581 gagtgacgca ccaaaaatgg gggcacaagc aacgatttgc gcaggcggac gttatgacaa
    14641 tctagtggaa gaactaggtg gtccttcaac acctggcttc ggatttgcaa tgggtatcga
    14701 gcgattactc ttgactatgg aggcagagga agtagttatc cctgcattta atgagttgga
    14761 tgcttacgtg gtcgcactcg gagacgaaac aaatatcgaa gcattgaaag tggtccaagc
    14821 gattcgaaac tttggttttt cagctgaccg cgacttcatg aaccgtaaag caaaagcgca
    14881 atttaagaca gcagataaag cgaatgccaa attagtattg gtgatcggtg aagatgagct
    14941 tgctagtcaa acgattaatg tcaaagcaat ggcaagccga aaagaaaaaa gttttccatt
    15001 agaagagata tatgaacatt ttgatgaagt ctatgatgaa atgactttat tcgagtagga
    15061 ggaaaaaaat aaatggcaca aagaacgatc tattgcggat tagtttccga acaacaatta
    15121 gaacaaacag ttacattaaa aggatgggta caaaaaagac gagaccttgg gggagtaatc
    15181 tttatcgacc tacgtgatcg tgaaggaatc gttcaaatcg tctttaatcc tgaaatcaac
    15241 aaagaagcat gggaaatcgc ggataaatgt cgcagtgaat acgtgatcga agtgactggg
    15301 gaagttagaa gacgcggaga aaatgcagta aatcctaaaa tggccactgg tgaattcgaa
    15361 gtgattgcaa gtgaaatcgt gattttgaat caagcaaaaa ccccgccatt tcaaattgaa
    15421 gatgatcaag caatcagtga cgaaatacgt ttgaaatatc gttatttgga tctgcgtcgt
    15481 ccaaaaatga ctaaaaacat caaattaaga aatgcgacaa caaaagcgat tcgtcattat
    15541 ttagatgata atgactttct cgatatcgaa acaccttacc tagggaaatc tacaccagaa
    15601 ggcgcaagag actacctagt tccttcacgt gtccatgctg gacatttcta tgcgttgccg
    15661 caatctcctc aattgtttaa acaattgctg atgaatgctg gattcgaccg ttattatcaa
    15721 atcgttcgtt gtttccgcga tgaagattta cgtggagacc gtcagcctga atttacgcaa
    15781 gtagatattg aaacaacgtt cttaacagca gaagaaatcc aaacttacac agagggattg
    15841 attgcgaagg taatgaaaga agttcgcggt atcgaagtca cattgccatt tcctcgtatg
    15901 acctatgatg aagcaatggc tcgctacggc agcgacaaac cggatacacg ttttgatatg
    15961 gaattgatcg atctaagcga tacagtcaaa gaagtagaat tcaaagtctt ccaaatggct
    16021 cttgaaaacg gcggtgtcgt gaaagcattg aatgctaaag gcgctgcaga tcgctactca
    16081 cgaaaagaca tggatcagtt aggtcaatat gtaggacaat tcggagctaa ggggcttgca
    16141 tggctgaaag tagaagaaga cggattgaaa ggtccaattg ctaaattcat gggagaagca
    16201 acagaagcaa tcattaaagc aacagatgca aaaccaggtg acttgctgat gtttggcgca
    16261 gataaaagtg aaatcgtcgc tgctgcatta ggtgctatcc ggacacgttt aggaaaagaa
    16321 ttaggactaa ttgatgaatc gaaattcaat ttcttatggg taactgattg gccacaattt
    16381 gaattttcag aagaagaagg tcgatatgtc tctgctcacc atccattcac gatgccaaaa
    16441 gaagaagata tcccgttatt ggctgaagac cctgcaaaag tctatgcaga agcttatgat
    16501 atcgtcttga acggttatga attaggcgga ggttctatcc gtattcatac tcgtgagctt
    16561 caagaaaaag tactagaaac tctaggcttt acaaaagaac aaatggaaga acaatttggt
    16621 ttcttgttaa cagctttaga ttacggattc cctccacatg gcggtattgc attaggattg
    16681 gaccgttttg caatgttatt agccggagaa gataatattc gtgaagtcat tgcctttcct
    16741 aaaaacggaa aagcagcaga tgtgatgagt gatgcaccaa gtatggtttc gcctttacaa
    16801 ttgttcgaat tgaatattga tgtaacgaat atagaagaat agccgataaa aaggtaaaaa
    16861 ggatttgtcg aatacgcttc ttttgacaac cttgatttaa ttgaaaaaca ctttataatg
    16921 aagccataac atataaagcg gggcttttcc atgaaaaaaa cattcgaaaa ttgggcttat
    16981 catgattaca cgaccaagct ttcggtatct atcgtttatg cgttgatcgc atcagtcgca
    17041 ttgaatttct tttatcagcc aggacatatt tattcaagtg gtattactgg tctagctcag
    17101 attttgacga ctttgtcaaa atcactgata ggattcgaag taccggtatc aacaactttg
    17161 tatttactga atataccttt gtttgtattg gcgtggttaa aaatcgggaa gaagtttaca
    17221 attttcactt ttatcacagt cactttgacc tccctttgta tccatatttt accggaaaca
    17281 gtcctatcta ccgatccaat catctgtgcg ttgtttgggg gggctgtgat gggactaggt
    17341 attgggctag cgttgagaaa cggcctatca tcgggcggtc tagatatcgt gagtatcacg
    17401 attcgtaaaa aaacaggaaa gtcgattggt tctatttcga tctacttcaa tgcattgatc
    17461 gtttttgttg ccggaattct atttggctgg caatatatgt tttacagtac gttgtctatt
    17521 ttcgtcagtg gaaaagtaac tgatgtcgtt tatactaaac aaaagaagat gcaggtaatg
    17581 atcgtaacga aacatccaga aaaagtagta aatgaaatcc aaaactgtat gcgtagaggg
    17641 atcacgatca tcaatgaagc agaaggtgct tataagcatg ataagcagac agtattgatg
    17701 acgatcgtta cacgatacga actgcctcta cttagagaag caatgaaagc ttctgatcca
    17761 caagcttttg taagtattgc agaaaacgtc cagatacttg gtcgtttcta tgaggatggc
    17821 atttagatcg agtttgacaa aaattcatga aatactgctg tagacagacg ttcgtttgtc
    17881 tgcagttttt ttctaccata gtagaatcag caaagaaaaa ctgcttttt
//
LOCUS       DAHUVV010000051        17735 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.052, whole genome shotgun sequence.
ACCESSION   DAHUVV010000051 DAHUVV010000000
VERSION     DAHUVV010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.052"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(205..570)
                     /locus_tag="NK767_001726"
     CDS             complement(205..570)
                     /locus_tag="NK767_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694721.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            778..1260
                     /locus_tag="NK767_001727"
     CDS             778..1260
                     /locus_tag="NK767_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694722.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1457..2347
                     /locus_tag="NK767_001728"
     CDS             1457..2347
                     /locus_tag="NK767_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HCH6694723.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(2634..3908)
                     /locus_tag="NK767_001729"
     CDS             complement(2634..3908)
                     /locus_tag="NK767_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCH6694724.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            4181..6652
                     /locus_tag="NK767_001730"
     CDS             4181..6652
                     /locus_tag="NK767_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCH6694725.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HANQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(6755..8491)
                     /locus_tag="NK767_001731"
     CDS             complement(6755..8491)
                     /locus_tag="NK767_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCH6694726.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(8488..9192)
                     /locus_tag="NK767_001732"
     CDS             complement(8488..9192)
                     /locus_tag="NK767_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6694727.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            9323..9826
                     /locus_tag="NK767_001733"
     CDS             9323..9826
                     /locus_tag="NK767_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HCH6694728.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            9786..10124
                     /locus_tag="NK767_001734"
     CDS             9786..10124
                     /locus_tag="NK767_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCH6694729.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10145..11563
                     /gene="ffh"
                     /locus_tag="NK767_001735"
     CDS             10145..11563
                     /gene="ffh"
                     /locus_tag="NK767_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCH6694730.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            11675..12253
                     /locus_tag="NK767_001736"
     CDS             11675..12253
                     /locus_tag="NK767_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCH6694731.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            12257..12778
                     /locus_tag="NK767_001737"
     CDS             12257..12778
                     /locus_tag="NK767_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCH6694732.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(12857..13411)
                     /locus_tag="NK767_001738"
     CDS             complement(12857..13411)
                     /locus_tag="NK767_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCH6694733.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            13664..13939
                     /gene="rpsP"
                     /locus_tag="NK767_001739"
     CDS             13664..13939
                     /gene="rpsP"
                     /locus_tag="NK767_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCH6694734.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            13951..14202
                     /locus_tag="NK767_001740"
     CDS             13951..14202
                     /locus_tag="NK767_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HCH6694735.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            14327..15118
                     /locus_tag="NK767_001741"
     CDS             14327..15118
                     /locus_tag="NK767_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694736.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            15288..15809
                     /gene="rimM"
                     /locus_tag="NK767_001742"
     CDS             15288..15809
                     /gene="rimM"
                     /locus_tag="NK767_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCH6694737.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            15799..16560
                     /gene="trmD"
                     /locus_tag="NK767_001743"
     CDS             15799..16560
                     /gene="trmD"
                     /locus_tag="NK767_001743"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCH6694738.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            16696..17043
                     /gene="rplS"
                     /locus_tag="NK767_001744"
     CDS             16696..17043
                     /gene="rplS"
                     /locus_tag="NK767_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCH6694739.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(17175..>17735)
                     /locus_tag="NK767_001745"
     CDS             complement(17175..>17735)
                     /locus_tag="NK767_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6694740.1"
                     /translation="DFTTSHNKRYSLLFQWLYLTGMRSGEAIALSKDDVHITNNNASV
                     VINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNPNGQFLFQTT
                     KGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKLAELGTPLYAIQDRVGHEN
                     SDITEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT         5812 a         3188 c         3616 g         5119 t
ORIGIN      
        1 aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat tattgctgca
       61 attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa atagaaaaac
      121 tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga aagtagcttc
      181 tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag ataaggctac
      241 cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa gcaagatcag
      301 ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg cgcaaaataa
      361 ataggaagtg acagtagata taatattggc aatgaataaa gatataatac gcctgttcct
      421 ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc cgcttacgga
      481 atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag ataaaagcaa
      541 cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa gtgttattat
      601 tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc attatttcta
      661 gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc cccctaattt
      721 ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg aacgttaatg
      781 agaagaaaag cagtaataga tcggctttat ttattttttt ggttttttcc tatcgttttg
      841 ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg cctgatccaa
      901 ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta tttagtgata
      961 agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca gctgctttac
     1021 ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt gattatcggc
     1081 cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat gtatttcttg
     1141 acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac atttttttac
     1201 ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa taactattga
     1261 tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc tcatatgctt
     1321 tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat aacttaacta
     1381 tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta gttgtaaata
     1441 agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg gaattctttt
     1501 agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa ataattcaag
     1561 tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac agccgttagt
     1621 cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct ctgtacaagg
     1681 aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta tcggaaactc
     1741 tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg atcataaagt
     1801 agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag atatcaccaa
     1861 acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag ttggcgggaa
     1921 agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg cgacattcga
     1981 aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt catcaggaac
     2041 agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt tctcatatct
     2101 cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag agaatgtagc
     2161 agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa aaccttcccc
     2221 tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag gacctgtcaa
     2281 agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg aaggaaatat
     2341 caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa aatcaactat
     2401 gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat attttgccgt
     2461 tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa aaaagagcta
     2521 cgacatagaa ctgtcgtaac tttttttggc tcttcgtcaa atcgtgttgg tgaagacaaa
     2581 tttgaataaa acttagtccg atatgcggcg cgttctgtgc gccgcatatt tttttagttt
     2641 tcaaagatca gaccttgttg taaatagatc cgtgcccgaa agttataaaa gttgcgataa
     2701 ccaaacgcaa ttcgtttaat cagcttgatc ttgttattca gcccctcagt gacgccgttt
     2761 gagtgggaca ccttaaaggc atttgaaata cccgattggt agcaatggaa gatcgtgaat
     2821 ttctttttaa acgactgagg taacgtagat gaaattgttt taagggtttc ttgaaagcct
     2881 tccgtacctc gtttaacgaa ataatagcgt aataattgac aaaaatgata cgcagttcgc
     2941 aattcttcgt tgtagcttaa cagctcatca acgatttctg tttgactaat tggtcgttta
     3001 aacagtcgat gataatgccg tttgagtggc tcccatgtcg tggaatcttt gagtaagagt
     3061 ttccaatatc gcttgagccg acggtacttc ttctgatttt cagagtgtga ggttcgaaaa
     3121 cgattcatga tctggacacg taattgattg aagctgcgat tgatgtgttg gacaatatga
     3181 aagcggtcag ttacgatctc cgcatttgga aagactgttt taagcaactg acaatagctg
     3241 gcattcatgt ccatcactag gtacttgacc tttaaacggg cctttctagg aaagcgcagg
     3301 aagtattgag ttagtttgta aagtctgcga tcttcaagga tcgtaaatag ctgattggtt
     3361 gtcccatcaa cacaaataaa actcatggaa ccagaaacag aattcattga tttgaactca
     3421 tccatacaca acaccgtcgg aaggaagttc atacgtggct ggtaatgctt cgttgcttca
     3481 cgcaagatcc gtgtcaccgt cccatccgag acgaagtact tcttagcgat tagtttacgt
     3541 gattgatttt cgtacagttc catgagaatc gcctgcttca gttcctttgt tagttgatga
     3601 tgttcatcaa ctaagtccgt tttcgcagaa aaggtccttc cacaatcatg gcagagaaaa
     3661 cgggtacgtt ttaattctaa aatagttaac ttttctttga ctttcaacat ctgagtgcgt
     3721 gtcgtatagg aaccgttgcg gataatacga accgatcgac agtgttcaca ataggaagaa
     3781 gagtcatcca attttccttt aataaactgc gcttgaacgc ctttatattc tttttcaatc
     3841 agccattctt catcaaaaaa taaatgttta tctgttaatc cgagtaattt tctagtatga
     3901 ttatccattg agagactcct ttggtaggat gacgttttgg tcgactaaat tttaccaaaa
     3961 gtgagtctct ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt
     4021 tccaccaaca caaaatatta tagaaccctt tttttttgct atatgacagg aatgaaaggt
     4081 gtttgcaagc ttaatgtgag gaacactttc catatctgtt ctatactgga taggagaagt
     4141 ttggaaatat ccagaaaacg aaatcaatcg aggtagccac atgaagaata aatggataaa
     4201 tcgaagtaaa aaaaagaaag taacacaaag atcatcagat aaagtcatcc gtctttttcc
     4261 atcgtataaa gaaggattaa cggcagatca agtcaaggaa cgaatagaaa aaggtgcagc
     4321 aaataattca gtcgatccca cattcaaaac aaatcagcaa atcgtattgg aaaacatatt
     4381 tacgtatttc aatttgatct ttcttatctt agccattttg ctttgtttag tagaatccta
     4441 taagaacttg acttttttac cagttatcat agccaataca ggaatcgcca tttttcagga
     4501 aattcgatcc aaaaagatct tggacgaact aaatgtttta aatacagcaa aagtcaatgt
     4561 catacgagac gggatagaac aaacaataga tatagaagac cttgtaatag atgacattgt
     4621 tattttagag actggatatc aaattccagc agatgcagag gtcgtggaag ggaaactgca
     4681 agttaatgag gctttattga caggcgaagc agatgaaata accaaagaaa taggtgatcc
     4741 attaatgtcc ggcagtttca ttgtttccgg aaaagcttac gcccgattag acaaagttgg
     4801 agcagattcg tatatttccc agctgacagt caaagccaag acaatgggag aaggagaaca
     4861 gtcggagatg attgcttctc ttaatcaatt gatcaaatgg attgggatca tcattattcc
     4921 tatcggtatc ctgctttttt ctcaaagtta cttttttaat ggaaacacga tcaaagaaag
     4981 tgtcgttgca atggaagcag cattgatcgg tatgatacct gaaggactgt acttgctgac
     5041 gacgattgca ttagcattaa gtgcgactaa actagcaaag caaagagttc ttttacacaa
     5101 tatgaaaagc attgagacat tggcaagggt aaatgtcctt tgtgtagata aaacaggaac
     5161 gatcacagaa aataaaatgt ctgttcaaaa aatgatcgtc tctaaaaaac aagaaataat
     5221 cagtcagtca gatcaattgg aagcatgcat tgctgactat gcaaaagcga tggcaggaga
     5281 caatgcaaca atggaagctg ttaaagcata ttttacaaaa accactgatc aatcctatca
     5341 ccatgttatt cctttttctt ctgtccaaaa attcagcagt gtagcgttgg cagacaaggt
     5401 gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat atgcggaaga
     5461 gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt acgatgggat
     5521 tcttgaagaa ccagtattga cagaagcagt cacaccatta ggctatattc tgattgctaa
     5581 tcctatcaga aaagaagcaa gagccacatt tgattacttc aaaaaacagc aagtagccat
     5641 caaagtgatt tctggagata atccgctcac cgtatccaat gtagctgtcc aagcagggat
     5701 ttcacatgct aatcagtacg ttgatgcatc taaattatca gaagaagaat atgaagcagc
     5761 tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa caaaagaaaa tcttcgttca
     5821 attgataaag cgtaacaata cagtagctat gacaggtgat ggcgtgaatg atattctagc
     5881 catgaaagaa gcagactgca gtatagcgat ggcttccgga aatgaagccg ccatgcaggc
     5941 ctcacaagtg gtcttacttg attctgactt ttcacgcatg ccagaagttg ttgcagaagg
     6001 acggcgcgtt gtgaacaata ttgagcgatc agccagcttg tttttagtga agaatatttt
     6061 ctcctttttg ctctcactgt tttcagtgat ctttgctctg acctatcctt tagaaccatc
     6121 acaaatcaca ttgattagtt tgtttactat cggacttcca tctttcttgc ttgctctaga
     6181 agaaaacaaa aagcgaatca gagggaaatt catcatgaat gtgatggaaa aagctgtgcc
     6241 aggtggacta acagatatga tcgtagtcgg tgcgttggtg atctgcgggg tgactttgaa
     6301 tctaaataaa acagatgttt ctacagcatc taccatgctt ttgattgcag tagggttcct
     6361 cgttctctat aaaatctgtt caccgctgaa taaattcaga agtcagatca ttcttttctg
     6421 tgctagcggt aacttttttt cggtcatctt tttgcataag ctgttttcaa tcaccggtat
     6481 ctcagctgta tcacttttgc tgttgagcat tctatttttc tcggcagatt cgatttttag
     6541 gcatttgacc acattggtag aatatttgtt tgaacgtaaa aatgaaaaag agcaaaagcc
     6601 gtttagtcgc gtagctaaaa aatttctatc gatatttaaa cgagaaaatt gacgaaaagt
     6661 atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc tttcgaagca ggatgagtca
     6721 tccaacttta aaacgcacga gcagttgctc ttttttacat tttcggaatg gtaacggtga
     6781 aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt accattaagg agttgcacgt
     6841 agtcttttac aatcgctaac cctaatccgg tcccgcctga atgtcggctt cgcgccttgt
     6901 ctacgcggta aaaacgctca aagatccgtt cctgatcctc aatgtcgata cctataccaa
     6961 agtcttggac agaaaacgat agatcatctg ttgcctgata gcggataata atctcgctgt
     7021 cgctttttga atattggaca gcattttcaa tcagattttt gataattgga taaaggatat
     7081 ctgttttagt agtaaatggt tcgttctttc caatcaatcg aatcgtcaac cgtttctttt
     7141 cgatgattgg ctgataattt tgaacgattt gctggaaata aggttctgta taaattgtct
     7201 ggatctcgta agaattctcg ccatcttttg acagctggat gatttcccga atcagtttat
     7261 ctaaccggat cgcatctttt tgcatgatct caagaaaaga agtcagagtt tgcgggtcat
     7321 cttttgcacc atccagtaaa gtctcggtaa aaccaatcag tgaagtcaca ggagttttta
     7381 attcgtgaga gacgttgcca acgaaatcct tttgtagctt ttctaaacgt cgcactttcg
     7441 ttaagtcata cgccacccct agaatctgtc cggtaccttc tgaattagta aagtagcgca
     7501 aactcaagtc taatgtctgc cgaccttctg taagggtgat ctcctgatgg atcagcggat
     7561 gctcaggagt cacttgatga atcagctgaa tgagttttgg ctcttgaatg acttctgtgt
     7621 accgctgttc aggttgatag cttaccacgt ttaaatgtag ctgcatcttt ggattaagca
     7681 gtagtaaacg agaatcagca tcatcaatca ggaatacgcc aatcatcagt tcgttcagta
     7741 acgtatataa ttgctcttct gtggtagtac acgcccgata catcttactc atttgttcgc
     7801 tcaacgcatt aatcgtctga tacaattctt cccattgatc gggtgtttgc atgattactt
     7861 ccgcctgatt aggattttgc accatttttt ttagtacggg cagcacggtt ttcaatggct
     7921 ggtttttttg atagatcaga taatagatca ttcctgcaag aacaaggaag aaagacagaa
     7981 agaagaaaaa gacccaacgg cgaaaactct cagttctcgg taaaaaaacg cttgtcggtt
     8041 ctgcgatacg taaaatggct tctaactggc cacttttttt gactggtaat gcgacatata
     8101 aaagatcctc attcaaagta gtacttttgc gcaaagcgct acctaggttc ccaccattca
     8161 atacggcttt gatttcggga cgggagtttc gctgttcatt caatgtttca ttgtttgtgt
     8221 caaataagat ctttccagta gcatccatta aagtaatccg ttcgttcgac tgatggacga
     8281 attcttccaa agcagttttg ttttgcgctg cttggaggtt ttcgacatct aattgccgga
     8341 tcagcagttg tgcttttttt gtcaggtagc tttcttgctg ttccacgatc tgctgttgaa
     8401 aataatggga aatcaattgc cagccaccaa aaaacaaact aactaaaaga ataagaaaga
     8461 agagacgttc ataaattttt ttccatttca tcgtttgggt tcctgaaact tatagccaaa
     8521 tcctctaata gtcaccaagt actttgggtg ttttggatct cgctcgattt tttctcgcaa
     8581 atgactaacg tgtacatcta cgattcggct ttgtcctgcg aagtcaaaat tccaaatgcg
     8641 gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt ttcatgaaat aaacaagtaa
     8701 ttcaaattct ttcggtgtta gttcaatcag atggtcgtct acggtcactt gataattggt
     8761 caaatcagca gtgatttgtc caatcgatag atattcaggc tcagcttcat cttttgattg
     8821 cttacgaggt tcaattcgtc tgaatattgc tttcatacgg gctaatactt cgcgaggact
     8881 aaatggtttg gtcaaatagt cgtcagcacc tatttctagg ccaatgatcc gatccacttg
     8941 gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt ttttcttgac gcagccgctg
     9001 agtgatctcc atgccatcca tcgttggaag catcacatct aaaatgatga aatcaaatga
     9061 ttgttctaat gctaattcta atccttcgcc tccatctgtg gcactggtta ccttgtagcc
     9121 ttctttttcc aaattgaacg tcaagagcgt tacaatagaa ggttcatcat caacaactag
     9181 tatttttttc attcaaatct cctttaaata ggtttcattc tgtttatatt ttagcataga
     9241 atcagtacaa agggctattt tacaaaagaa ataaatggta taataggaag gcactttgct
     9301 gaatagaaag gaagagcagt caatgattgg aatcagtgca tgtttaggcg gtctggcctg
     9361 tcgctatgat ggacagaaaa aagaagtacc agaattgatg gaactagttg aaactggaga
     9421 agctgtcatg gtctgtccag aagtgatcgg cggattacca attcctagag atcccgctga
     9481 aatcattggt ggaaatggat tcgatgtgtg gaaagatcaa gcaaaagtat ggacgacaag
     9541 cggcgaagat gtaacggaag cgtacaaaaa tggcgcgatc aaagcctatc aaaaattgca
     9601 ggaaaaacac attgatctag tcatcatgaa ggaaaaaagc ccttcatgcg gaacccattc
     9661 gatttatgac ggaagttttt ccggaataaa aaaaaatggt gccggagtgg ctactgctta
     9721 ttttattcag caaaaaatga ctgtgctctc tgatgaagaa tggctgaaaa ctagaggtga
     9781 gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct ctttgaattt tattcgacct
     9841 tattgacaga aaaacaaatg aactacatgg aattatatta tgcagatgat ttttcattag
     9901 gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt atatgacaat atcaagcgga
     9961 caagcaagat cttggaggat tatgaaaaaa aactccatct tttttctaat tatattgttc
    10021 gagaacaggt attggaaaat atgactgcat atattacaga taagtacccg gaagataaaa
    10081 aactacttga atatgtacag caaattcaag caatcgaaga ataaataaaa ggaatgaaga
    10141 aaatatggca tttgaaagct taactgaacg tctccagcag gcgatgggaa aaataagaaa
    10201 aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg cgggaaatcc gccttgcctt
    10261 actagaagcc gatgttaact tgcaagtagt caaagatttt actaagcgcg tacgcgaacg
    10321 tgcggtaggg gcagaagtgc tggaaagttt atcacctgca caacaaatcg taaaaatcgt
    10381 tgatgaagaa ttgacgatca cacttggttc tgaaacagca gagttaaata aatcaccaaa
    10441 aataccaact gttatcatga tggctggtct tcaaggggca ggtaaaacga cctttactgg
    10501 taaattagcc aattatttaa agaaaaatga aaatgctcgt ccgctcctaa tcgcagggga
    10561 cgtatatcga ccagctgcga tcgaccaatt gaaagtatta ggtcaacagc tagacgttcc
    10621 tgtatttgat atgggaactg aagtcagccc ggtagagatc gtccgtcaag ggatggagtt
    10681 agcaaaagaa aagaaaaacg actatgtatt gatcgatacg gcaggacgtc tgcatatcga
    10741 tgaaacattg atggatgagt tgaaacaaat caaagaattg acgcaaccaa atgagatttt
    10801 gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac gtagctgata gcttcaacca
    10861 gcaattaggt attacagggg tcgtcatcac taaacttgat ggggatactc gaggcggggc
    10921 agcactatcg atccgctcag tgacaggcgc accaatcaaa ttcatcggtt ctggtgaaaa
    10981 attgactgac ttagaagtat tccacccaga tcgtatggct agccgtatcc ttggtatggg
    11041 ggatatgctg acattgattg aaaaagcgca gcaagactac gacgagaaaa aagcggaaga
    11101 acttgcacag aaaatgcggg aaaattcatt cgatttcaat gactttattg aacagcttga
    11161 tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa atgatcccag gaatgaatca
    11221 agtccctgga atcgaaaacg tcaaggtaga tccaaaagat gttgaacgca aaaaagcgat
    11281 ggtttattct atgacgccag cagaaagagc aaatcctgat ctgttgaacc caagccgtcg
    11341 tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa gtgaatcgaa tgatcaaaca
    11401 attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa ggggatatga acatcccagg
    11461 tatggaccaa atgtttggtt caggcgtcaa aggaaaacta ggcaaaatgg cgatgaatcg
    11521 aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa taagatgaaa aaaacagctt
    11581 cggtacagac tgaaaatgtc tactgccgga gcttttctat cagcaatttt tattctttac
    11641 taatacaatt actctaaacg aagaggtggc acagatgaga tgtccgtgga gtgaacaaac
    11701 agaaagtatg cggcaatatc atgatactgt ctggggtgtt cctgaatatg atgaccaaaa
    11761 gctgttccga aaattgatgc ttgatattaa tcaagccgga ttgagctggc agacgatttt
    11821 aaataaaagt gaagcattcg atgaagcata tgatcgattc gatatcaaaa aagtcgcaaa
    11881 ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa ggaattattc gtaaccgaag
    11941 aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg aaaattcaag aagaaatcgg
    12001 cagtttttca gagtatcttt ggtcgttttc taatggaaaa atcgttgatt cttcttatca
    12061 ttcacaagaa gaagtcccaa caacaagtcc tctttcagat gaattagcaa aagatctaaa
    12121 gaaaagagga tttaaattta tcggaagcac aacaatctat gcttttttag aagctgtcgg
    12181 gatcatcaat gatcaccttg acagttgttt ccgcaaagaa gaggtcgtac gtatcggcag
    12241 aaagggcgaa taaagcatgc gaaaaatcat attttatggt gctataagtt tagatggcta
    12301 tttggcaacg aaaacggatg atcttcaatg gctttttgac actccaacag gtaagcaaac
    12361 tacttatgaa gctttttatg aaacaatcga tacgaccatc atgggcagaa aaacttatca
    12421 ggaagcaaaa aaatatttgg agaccgaagc aatttatcca gagaaaaaga atttcgtttt
    12481 ttccacccac gcacaattac aattgccaga tgctgaggtc gtaaacgaag atcctgttgc
    12541 ttttctaaaa aatctacaaa tgacagaagg aaaagatatc tgggttgtgg gtggcggtcg
    12601 attgatcaaa ccgctattgg aacatcaaat gattgatgaa tggtatatcc agattacccc
    12661 tgtattatta ggggacggga ttcctttatt ccaaaaagga acatatgaag cgcgttttga
    12721 aatgctagac acgacacgat tcggtgaatt tatcgaattg cattatgtac gaaagtagaa
    12781 gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc tgtacatagt tgcctttttt
    12841 attaccgtta tgctttttac tctgttaatt tgttagaaaa atctagatac gcatctaaaa
    12901 tcgtttggaa gaaacttaat gttccttcaa cgatattacc attttcatca attagattcg
    12961 caacgtttcc gatatatgct tccggttgct gcaaggttgg cacattcaaa aagacaagtg
    13021 attggcgtag atggtggttt gcgccaaagc cgccgatccc acctggtgat gcagtaacga
    13081 ctaaacctgg tttatgatcc caaacactgt gtccataagg tcttgagcct acatctaaag
    13141 catttttcaa tactgctggc acagaacggt tgtattcagg agtaacgaaa acgacaccgt
    13201 ctagtcccct cacttcctca cgaaaacgag tccattcagc aggagcctgt tctggagttt
    13261 cgagatcctc attgtaaaat ggcagatcat cgatttttac aaatacactt tcgtaccctt
    13321 caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt gtaagaatct tttcttaaac
    13381 taccaataaa aaatccaatt ttttttgtca tacgattttt cctccttcaa actatctata
    13441 ataatcttac aaaagataag taactggagc aaataaaaac acttactgac aaaagctaca
    13501 tagaagatag attacttatt tttaacaaaa aagaagtcta aaatcttcta ttttctaaaa
    13561 aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta cagggtaaaa aaaaactgtt
    13621 acactacatc ttgtgaataa aacaatggag gtggaaataa ataatggcag ttaaaatccg
    13681 tttaaaacgt atgggttcta aaaagagtcc gttttaccgt atcgtcgtag ctgattcacg
    13741 ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact tacaatcctt tgaaagaccc
    13801 tgcagaagta gttttaaaag aagacttagt tcttgactgg ttatcaaaag gtgcacaacc
    13861 ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt atgcaaaaac accacgaagc
    13921 taaattctca aagaaataag gtgaccggat atgaaagatt taagcgactt agtcttaaca
    13981 atcgttcgtc cgctagtcac ttatcctgat caagtcaagt tagacgtcgt agagtcgaaa
    14041 gacttttacg agtacaatct aacagtggct cctgaagata ttggccgtat cattggaaaa
    14101 caagggcgtg ttgctaaagc aatccgcaca atcgtttacg gtgttcggat caatgcaccg
    14161 aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt aaataaacgt gctgtactcg
    14221 aattttcgag tgcagctttt ttgttttttt tattaattga agaaatgatt atttggcaaa
    14281 acaagcaaga aagcgtatcc ttataggagg agggagtgaa cagccaatga gaaagaaaat
    14341 gttatttatt ccatttgctc tattgttaag cgcttgtagt tcggttactc aagaatcaga
    14401 caatacacat ctatcaagca gtatatctga aactgttacc agtccagaaa aaagtagtac
    14461 caaaacggct tccaccactg aacaaacaat ggctacctca aataatgaga aaaagactgc
    14521 tttagaccag ttgaaagaac aacagccaaa tgttcccatg cctctagatg taccagtctc
    14581 ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa ggttattcta ttttatatta
    14641 cagaacagat cgtcccctcg ggttgaatgc tgacgaattg aatcaagaaa cgcctattgc
    14701 aacctatcta tatcaatatg ggtttgcttc tagtcaagaa acaatccaag ttttgcagcc
    14761 atttgagatc gacacaaatg gtcagcaagt ggacttaggt tctcgaatca cagggtatca
    14821 gcaaggtgca gcaggttcaa gttttcttga atggcaagaa ggaaactggc gtattcgaat
    14881 ccgtgggaat aatatagaag gccaagatcc cttactatta gctaaagaaa tcgtagctta
    14941 tttggaagaa aacagtctac cagcacctga acaatttggt aaaattactg ttgatatggg
    15001 tgatacgacg aatcgagccg tagaagtaag ctggcaagaa ccgaaaaatg cctatacgat
    15061 cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt tctatgaaga ggttgtgaaa
    15121 tcagcgcgct gatctgagcc catcatttaa ctccaagtca caggaagtaa tcaaaaatag
    15181 cacgccgcat ttttgcataa ttttggctta ataccttaaa agaaaactag aattgcctct
    15241 tcacacatgc taaaataaaa cagacaaaaa agaaatgggg atataagttg actgaatact
    15301 tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg cgaagtccgt gttatctcaa
    15361 caacggattt cccagaagaa cgttacaaaa aaggaacggt gctaacacta ttccaagacg
    15421 gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa acataaaaat tttgacttat
    15481 tgagttttga aggacatcct tctatcaatg atgtggaaaa atatcgggat ggcatattaa
    15541 aagtgtcaaa agacaactct gtagatctag ctgaaaatga attctattac catgagatca
    15601 tcggtctgca tgtattagaa gatggaactg agttaggtaa agtaaaagaa atcttgtcac
    15661 caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa agctgatata ttgcttccat
    15721 atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg agtggtccgt gtagaaattc
    15781 cagaaggact gattgacgat gaagattgat gtattgactt tatttccaag aatgtttgaa
    15841 ggtcctatgg gggaatccat catcggtaag gctgtagata aaggattatt agatatcaat
    15901 atctctaact tccgcgatta ttcagataac aaacatcaga cagtagatga ttatccatac
    15961 ggcggtggag caggtatgct gctcaaagtc cagcccatct atgataacat ccaagccatt
    16021 gaaaaagctg caccggatgt caaaaaaaga gtaattcttc ttgacccagc tggtaaacga
    16081 ttcgatcaga aaatggcaga agaattttca caagaagaac acttagtatt catttgtggt
    16141 cattatgagg gatatgatga acgtatccgc acattagtga cagatgaagt ttccttagga
    16201 gattatgtcc ttactggggg agaattaggt gcgatggtca tgatcgatgc aacagttcgg
    16261 cttttgccag atgtgttagg gaatcagact tcagctcaaa cggattctta ttcaaccggt
    16321 ttattggaac atccccaata tactcgcccg gcagaatata aagggatgaa agtaccagaa
    16381 gtattgacaa atggaaatca taaattgatt gaagaatggc aattaaaaga atcactaaga
    16441 aggacctatc aacgtcggcc tgatcttttg gaaacacttg aatggacacc tcaaatgacg
    16501 aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa cagcagatga agccaagtga
    16561 ctttttctaa aatcttcttt acagtaggtt gataatatgt tagtatttta tggtgagtgc
    16621 aaaagcactt aactattacg atattccgct gtgagaaaag ctcataagaa tatttggaat
    16681 aaggagaaga aaaccatgaa tccattaatc gaagaattaa caaaagaaca attacgttct
    16741 gacatcccag cgttccgccc tggtgacact gtgcgtgttc acgcaaaagt tgttgaaggt
    16801 actcgtgaac gtatccagtt atttgaaggt gttgttatca aacgccgcgg tgctggaatc
    16861 agcgaaactt atacagtacg taaagtttct aacggagtgg gcgttgaacg tacattccca
    16921 ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct acggtaaagt acgtcgtgca
    16981 aaattgtact acctacgtgc attacacgga aaagctgctc gtatcaaaga aatccgtcgt
    17041 taatcatcgt tatcagacag agaaagaacc cttggtattc aagggttctt tttttattat
    17101 ctgttttttt atattaacga tttctatttg gtacattttt gattaataaa aaggggcaaa
    17161 aaaggggcaa caagttacag tttttctata tcttctttca atttttcttt tactcctttt
    17221 gtcacatgca aataaatttt ttcagtgata tcactatttt cgtgaccaac gcgatcctga
    17281 atggcataca gaggagttcc tagttccgct aattttgaaa tatgggtatg tctaaaaata
    17341 tgtgaactaa gttttttatc aatcttcatg tcagctttat gacttctcaa ataggtgtta
    17401 attgctgtta gttggaaagg agttcctttc gttgtttgaa atagaaattg accatttgga
    17461 tttaattcta gaagctcatt gtaaatagct atcgcttttt ttggtaaatc aatttctcgc
    17521 attccggcag ctgtttttgt agaatcagat tttttcatat cagctattga acgttctcta
    17581 tacatcatcg tcccatttat aactactgat gcattattat tggtaatgtg tacatcatct
    17641 ttacttaatg cgattgcttc accagacctc atcccagtca aatatagcca ctggaaaagg
    17701 agagaatatc ttttattgtg tgaagttgtg aaatc
//
LOCUS       DAHUVV010000052        17633 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.053, whole genome shotgun sequence.
ACCESSION   DAHUVV010000052 DAHUVV010000000
VERSION     DAHUVV010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.053"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            30..3308
                     /locus_tag="NK767_001746"
     CDS             30..3308
                     /locus_tag="NK767_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HCH6694741.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3849..5756)
                     /locus_tag="NK767_001747"
     CDS             complement(3849..5756)
                     /locus_tag="NK767_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HCH6694742.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5886..6761
                     /locus_tag="NK767_001748"
     CDS             5886..6761
                     /locus_tag="NK767_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HCH6694743.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6841..7815
                     /locus_tag="NK767_001749"
     CDS             6841..7815
                     /locus_tag="NK767_001749"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCH6694744.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            7995..9140
                     /locus_tag="NK767_001750"
     CDS             7995..9140
                     /locus_tag="NK767_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCH6694745.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9277..9621
                     /locus_tag="NK767_001751"
     CDS             9277..9621
                     /locus_tag="NK767_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HCH6694746.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9892..11178
                     /gene="efmA"
                     /locus_tag="NK767_001752"
     CDS             9892..11178
                     /gene="efmA"
                     /locus_tag="NK767_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HCH6694747.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11316..11915)
                     /locus_tag="NK767_001753"
     CDS             complement(11316..11915)
                     /locus_tag="NK767_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HCH6694748.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12319..13440
                     /gene="mnmA"
                     /locus_tag="NK767_001754"
     CDS             12319..13440
                     /gene="mnmA"
                     /locus_tag="NK767_001754"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCH6694749.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13686..15107
                     /gene="arcD"
                     /locus_tag="NK767_001755"
     CDS             13686..15107
                     /gene="arcD"
                     /locus_tag="NK767_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCH6694750.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15121..16239
                     /locus_tag="NK767_001756"
     CDS             15121..16239
                     /locus_tag="NK767_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCH6694751.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16299..17591)
                     /locus_tag="NK767_001757"
     CDS             complement(16299..17591)
                     /locus_tag="NK767_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCH6694752.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
BASE COUNT         5558 a         3196 c         3787 g         5092 t
ORIGIN      
        1 ctttctaatt tgttacggga ggaatacact tgttaaaaaa acgtataaaa tacagtgcgc
       61 taatgatgaa tctccttatg ctcagtaccc cggttttaat ttctgtcgtt gaagttgctg
      121 ctaacgaaca acagtctgtg aacgaagaaa atgcacacgt gaacattgaa gggatgtcaa
      181 caatggagga aataccaaga actagtgaag atatggatac ggaagaagga aatgatccta
      241 tgatgaaaga atcagaaaat gaaaagacaa aaaagcaatc tgaagaagca aatgaagagt
      301 ggaaaggggt tgttttcgga gaaagcacaa gtgctagtag gtatgccatt gaaccattag
      361 aagacggcgt gcggctctct tccacgaata acggcggaaa attccagtca agcggatcag
      421 atggaatgac ctattattat caagcaatcc ccaaagacat caactttagg atgcgggcga
      481 cgatcgaaat agaatcttgg acctatacga atgcccaaga aggatttgct ttaatggtaa
      541 gagacgcagt accaaaaaat catttgtttg ggcaggcatt ttattcaaat tcatttgcca
      601 tactaggcag tcggatcgaa tatgtctggg actcggatag acaagaagtg gtcaacacga
      661 gcggcagcaa atatacgatg cgacttggat tggggacacg ggcaatcaca ggaatatcat
      721 ctgaagatcc gcaagctgcg ccagctcctg ggagtgtctc agtggaaaca acacctttag
      781 agacttcagc agggttcttg gaaaaagaaa caggttctta taatatattc ggaaacggtc
      841 atgggaatct gtttccggcc acgaactcga ttactaaact ggacgtagag atgaaaaaaa
      901 cgaatactgg tttagaggca tattattacg atcctgaaac aggagaagaa atggcttctg
      961 acatcatgta tgactgggaa aaattatacg cttcagatga atcggttatt tatgcaggat
     1021 ttgctgcagc aaggaatatg acgatcaagg tagaaggaat cgaagttgct tactcagatc
     1081 ctgcgacaga tcctcctggg caagaaagac ctactcgctt gattgatccg atttacactg
     1141 tgacctctag taatcattca ggaaaccaag atcatactat gtcctttaga gcaaatgctg
     1201 acggattatt gacaattaaa aataaagcaa caggagaggt gcttaagaac gcaaaaaact
     1261 tggctatcac tgcgaatcgg gaatttgatt atcagacaaa actgatggaa ggaacgaacg
     1321 aatttacttt cagttttacg ccagataaaa actatccgcc agaagaatac gtagagatga
     1381 ctagttatga aacaaaagag attcttcata cagtggacta ccgtaaacct aaatattcga
     1441 cagtttatgt aacaccagaa gggtcagatg caggaaacgg aagcagacaa aatccattat
     1501 ctctgacaca agcattgaga tacgcatatg ctgggcagac aatcgtcatg gcgcctggaa
     1561 cttattcctt cgaaagagga ttgacgattc caaaaggcgt taacgggaca agagaaaacc
     1621 cgattcagtt gattgcagag aaaaatccag aaaacaagcg tcctgtactt gattttaaag
     1681 gaactggaaa tgggatgaca ctgttcagtc actactgggg tttacgaggg tttgacatta
     1741 caaatagtgc agctatgcaa aaaggattac agatttctgg aaatcataat ttgatcgaag
     1801 agattcatgc ttatcggaat ggaaatacag gaatccaaat cagcggtagc tcaaatgatc
     1861 ctttcgaagc atggcccgcg cataacttag tcaagaactg tacatctttt gaaaatgccg
     1921 atccaggatt tgaagatgcc gatggatttg cggccaaact gacaatagga gaaggaaatg
     1981 tatttgacgg ttgtatcgcg tatcataatg cagatgacgg ttgggacctt tttgctaaaa
     2041 atgaaacagg ttcgatcgga aaagtaatca tcaaaaactc agttgcttat cgaaacggct
     2101 gggtaccagg aattgaggga gaaggtaacg gtaatggatt caaaatgggc ggttcttctt
     2161 taacgggccc tcatgaattg atcaatagtt tgtctttcga aaatcttgcg aaaggaatcg
     2221 attccaatag cggaacagat attatcgtca atagcagtac aagttttaat aacggttcat
     2281 ataatgtggc tctttatacc tcttcagcag gaaacacaga ctaccatgct tctggtatct
     2341 tatctttccg aacagagaat cttgagatga gagagcagat ccgcccattg aatcaaaacg
     2401 aagatcaaat ctaccactcg ttgaactact attggaatga acgagagaaa caatcagaaa
     2461 atactcttgg acataccata tcaaaagatt ggatcgagtc tctaaataca tgttcgaaag
     2521 aaggtcagca gcctgttacg cgctatgaaa atggaagtat ccatgttcat ggactgatgc
     2581 agattaaacc ggggaatcgt caaacagaaa aggaaagagt ggatggtctt ccattatctg
     2641 taggtgctat ttttgacgga gaaacctcac cgtcttcgga atatccagag tatgcaatcg
     2701 tacaagaacc aaatcagaca aaaccaaatg aacaaaataa tccgattatt atacaagaag
     2761 caaatggtgt ggcggagaat gcagaaggaa atatcattga actgaagcct tttacagttt
     2821 ttgtgtcaac tttcacacca aatcaaggaa cgcctgtctt tgaagatatg cgttatttcc
     2881 cagaaaagat ccaaatcgtt gaaacacagt caagtgctca attttctaat cataccgcta
     2941 ttttttcagc accttcaaaa ggagaatata cgctagaagt cacttatcgt tcggaaatat
     3001 ttgatgggga aaaatggtct gctcatacag aatctgttac ccagcaaaaa gcgatcactg
     3061 tgcgagaaac tgccgatgtg cccccgggaa atgacaaacc agaaagagaa aatccaggaa
     3121 atgaatcacc aaatggaaaa ccaaatggaa aaccttctca tccggatagg acagcaaaag
     3181 aaagtgaaaa aaatggcact caaggactcc caaaaacggg agaatttaat aacccgttgc
     3241 tagcattagc tggaggaatt ttgttaatcg gtgtagttgt ttatgtcatg aaacagcgaa
     3301 aaaaataagt ggaatttctc cttttatgat gtaaaacaat tgactgagga aagtaaaaat
     3361 agaaggaaat gagaccaata gatgtctcac ttccttctat ctttttatag atgaggttct
     3421 ccttgctgct tttgattggc tgttcttatt acttgatgca ggacagcttt ttcacaatct
     3481 ctccactaaa cggtgttcaa tcagctgacc ctcggtatta gctcaacaaa cggcaaaata
     3541 tgaggaacta tttttccgtt tttttctcta atactcaggt caggacgctg atttcacaac
     3601 ctctcgataa acggtgttca aaagccagtt tctcggtagt aagtcaaatt tatgcaaaaa
     3661 tgtggaaagc cattttcaca aaatttttct tactacttga aactaagcag ctttttcaca
     3721 acctctaata catggtattc cagaagtaac ttcttcgaaa aataagccga aatgtcacaa
     3781 aaatttgaga agcgattttc ccaaattcct tcttatttct tgaagctgac cacttctggc
     3841 acaacctctt accagattcg cactcttttt tctggcgcta gatacatagc gtcttgttcc
     3901 gtgataccaa atgtttcata gaattcgtca aggttttttg gttgcacgtt tgctcggagt
     3961 ttagcagggg catggacatc gatttgcagt aaaagttgtt ggtattcttt ttttgctttt
     4021 gtacgccaaa tcgttgccca attgacaaag aattcctcga tagaaagatc tgattccgtt
     4081 tttgctgctt ccaatgcaca gcttagaccg cctgcatcag caatattttc cgaaacggtt
     4141 aattttccgt ttacttttcc gtcagcaaaa ggcagaccat cgaattcttc gatcattgct
     4201 tgtgctttgt tttgaaattg tgtaagatct tcttctgtcc accagttgtt caagttgcca
     4261 tattcgtcaa ataaagcgcc gttattatca aatgcatggg aaatctcatg agcgataaca
     4321 gctccgattc caccataatt agcagagctt gattgttcta gactgtaaaa tggtgcttga
     4381 aggatcgctg ccggaaagac gatcacattc cgaaatggat gataataagc attgaccgtg
     4441 tcagcactca tttcccattc tgaacgatcc actggtttgt tccatttgct gaaacgatct
     4501 tttaatgtga gtttgctgaa aacaagagta tttgaaagca aagtaccacc ctgatctgca
     4561 ggtactgttt tgaactggtc gaataatgct gggatcttat cggggtagcc tacttggata
     4621 cctaagttat ttaattttat gacggctttt tcttttgttg catccccaag ccagtcattt
     4681 tcttctagac gttttttata aacggcgatc atttttttca ccatttgttc tacgtctttt
     4741 ttagcagctt cgccaaaata tttttttccg tagtaatcgc caactacttg atcgaattgt
     4801 ccagaggcaa ggtaatacgc tgcttttttc tgaggcattg cttcgtctgt tccagacaat
     4861 acgcgagaat agattccgct gacttggcgg aattcttctg atagataacc actaagagaa
     4921 cgtactgttt taatgatcat ccagcttttg aataacggaa aatgagcttc tgtcaggatg
     4981 tcattcaaat gatcgaagta cactggatca gttacgatca ctttatctgg agttgtccca
     5041 atcaaacccg tgacaagttt tttcaagtcc agtttatctg tgtatttaat aaatgtatca
     5101 aaagattgcg gattatacat tttgctgtaa tctgcgtttt cttctgcact ttttacatga
     5161 ggagcaatca atttatcaaa ttgcactgct tgttccgtga ttgcttctgc ctgacttttt
     5221 tctttgccgg caagctctaa taactgaaca gccatatcaa agaaaacgcc tattagttgt
     5281 gcaccgttgg gatggtctga tgcataatac gtcttatctg gaaggaataa agaaggcgga
     5341 taagcaaaga aggcgttcgt ttgcgcattc ttcatatctg catctacatc cagattaaat
     5401 ggcaatggta atgtatccaa cagccattct ggaaattgct tattcaattc gtcgaaagac
     5461 tgcaatgatt caactttttc gattataggt tgaagcggtt gagcatgcaa ctcatttctt
     5521 cggtcataat cgtttgccag ttgataatat gcaaggaaat gtttcatttt ttctgaagac
     5581 actttttctg gatgggcaag cagatcgtct gtttctccca tcaaaagttg gtcgatcccg
     5641 tcaactaaat cttggaaacc gcctgtagct ggtttatctg cagggatttt tgctgttttt
     5701 tgccattctt cattgactgc ttcatagaaa tcttgtttca atcgttcttt gttcattcat
     5761 cgatcgctcc ttttttgttt cgtgtgccca ttatcttaca aaatgaagga attaacaaat
     5821 agaacataaa aaattgaaaa ttcgaacaat caaactgatt tgtttttttc aatccttcac
     5881 ttataatgaa aaaaaggagg cgatcccaac tgaatcaagt agtggataaa cctttatatt
     5941 tcaaagtaga taaaaaaatt aaaaaattag ccagcactca gcagctgcag tcaagaaaaa
     6001 gtaaattgct ttttttagct ctggtttttg aagatcaaag ttatgtgatt atcgatcaat
     6061 caggacatcc ggtagaatat tctccagcaa aatatactta tcaagagggt ctttcttgta
     6121 aaaaatggaa gctgatcaat gaagagccaa tcgagttatc tagatggatc aacagaaaag
     6181 aagatattcc cgttttgatc gaagaaaaaa ggtcaggaaa ggaactcgca aattgctggg
     6241 tgggattgcc agaagaacgt tttttgaggt ataagaaatg ggctaccccc tcaggctatc
     6301 tttgtggtac ttacgcggca gcagttctcc ttgcctatta tcaagactat cgtaaggggt
     6361 ggatgcttcc aaatgagatt cgcaagaaaa atacggctga ttcgttggtt ttgacgaagg
     6421 cattgcgtag ccagatccaa ccactaggac ttcctacgat tccttttcag gttagtacag
     6481 ggatttctag ttttttaaag aagaatggca atcatgagcg tgcacgagca actcttctag
     6541 gttcatggca gcgagctaca aaacgtatac gtgaaggcaa acctgtgatg attggtattt
     6601 tgaaagtact tggcagtaca tatggcaatc attgggtgac agcgtacgct tattttgaaa
     6661 ctgaaacagg agaacgttat tacaaagtac acgacaactg gggcgattat cataaagtca
     6721 ttcctgccag ttggagtaac ggaacagttt cacttcctta agaatttgct gaaattaaca
     6781 gaaaatcttg gaaaaatatg ttaaactatc gaagaataga gaagttttgg ggaggactac
     6841 atgagtaata agtatcagga tgacacgttg aagattttta gtttgaatgg caaccgtcca
     6901 ttagctgaaa aaatcgcgaa agtgttcgga acagaattag gtaaaagcgt cgtgaagcaa
     6961 tttagtgatg gcgagatttc catcaatatc gaagaaagta ttcgagggga tcatgtgtat
     7021 atcgttcaat caacgaatgc acctgtcaat gattactata tggaactact gatcatgatc
     7081 gatgcaatga agcgagcaag tgcgaaaacg atcaatgtcg tattgcctta ctatgggtat
     7141 gctaggcaag accgcacggc gaaacctcat gaaccgatca ctgcgaaatt gatcgctaac
     7201 ttgatcgaag aagcaggggc aactcgagta ctgactttag atctgcacac ggttcaagta
     7261 caaggattct ttgatattcc tgtagataat ttatttacga tgccgttgtt tgctcattat
     7321 taccgtcaat taggtctgac gggagatgac atcgtagttg tttcgccaaa aaacagcggt
     7381 gttcagcgtg cacgtagtct ttcagaatat ttgaacagca cattagccat cgtagatcat
     7441 gcagatgatg cttcaactga gggtggttat gtgatcggtg atgttcaagg aaagacatgt
     7501 atcatggtag atgatatctt gaacacagga gcaacgcttg cacgagcagc aaatgtatta
     7561 aaagaaaacg gcgcaaaaga agtatatgcc tgtgcgtcac atggattatt gtccgatcat
     7621 gcgaaaagta ttttagacaa tgcaccaatc aaagatattt gtatcactga ttctgttttc
     7681 acagaagaaa atcgtcatcc agaaaactta acaatcgtta catgttcaga attaatgggt
     7741 gaagcggtca aacgcatcca tgaaaataca ccgatgagtc cgttattccg tctggaagaa
     7801 aaagaattcg aataaacaaa aaaaagaggg aaagaagttg tgacagaagt gagcagcttc
     7861 aaggagctgc gacaagactt ttgtcacagc tccttttttc tatgcccatg aaacgtcctc
     7921 tagacaaaaa tgagctcttc tttggtttgt tcgctatttt atgctagaat gtgcctagca
     7981 ataatttgga agggatgaat gaagtgaaaa ctatttatct ggatcatgcg gcaactaccc
     8041 ccatgcatcc acaagtaatt gaagcgatga cggcaatcat gcaagacacg tttggaaatc
     8101 cttcaagcat acatggattt ggtcgccacg cgcatgaaaa attggaaaca gcaagacaaa
     8161 tcattgcaga cagtctgcaa gcaaaacctc acgagatcat ctttaacagt gggggaacag
     8221 aaggggataa tacagctatc ttagaaacag cgttctcgcg tcaaaaagaa ggcagacatc
     8281 taatcactac agctatcgaa catcctgctg ttcttaatac catgaaatat ttagagacaa
     8341 aggggtttga cgttacatat ctgcctgtag atgaaaaagg gaatctgtcg atcgaagagg
     8401 tcaaaaaagc gttacgagaa gatacgatct tggtctctgt gatgtacggg aacaatgaaa
     8461 tcggaaattt gatgccaatc aaagaaatcg gtgaactttt agcggatcat ccagcttatt
     8521 tccatacaga tgcggtacaa gcgtacggaa accaaacgat ccatccagaa gaattaggaa
     8581 tcgatttatt aagtatctct gctcataaaa tcaatgggcc aaaaggcgtc ggtttcttat
     8641 ataaacgaga cggagtaaat atccctgcat tgcttttagg cggggagcag gaagaaaagc
     8701 ggcgtgcagg tacagaaaat ttagcaggga tctggggtat ggcaaaagca gttgaactgc
     8761 tgtcacctga agaacgtgaa gcacgtaaca acaaatataa aggattccag caattgattc
     8821 ttgatacatt agaagcaaac gaaatccctt atcaagtaaa tggtgattta acgaataaac
     8881 tccctcatgt gttgaatctt cgcttaattg gcgaagcaaa tgatttgcta ctgatgaagc
     8941 ttgatcttaa tggggtcgct atttctactg gttcagcttg tacagcagga aatatcgagc
     9001 cttcacacgt attagaagca atgtatggag aagatacacc gatactaaaa gaatccatcc
     9061 gtatcagctt tggtttggga aataccgaag acgacatcca gtatttcctt gatcaattag
     9121 ttcgtgctgt caaaaaataa ctagtgaagt cattcttata tgaacagatg tctgagagaa
     9181 cgccttcatt ttttagggaa cttaactgaa aaacctagta aaaatcagga taaactttta
     9241 taataaagag aaactaattt tatcgaggtg aaaatcatgg catttgaaac aacagcaacg
     9301 gtactgggcg caccaattag ctatcgtttg catcctgatg caaaaagata tacattgcgc
     9361 gataatggat ttacagaaac aaacggaggc aattatcaat tgatccgacc attagatgcc
     9421 acgccgcaaa gcaaagaagg attcaaatta aaaatcactg tatcaaaaga tattcaaaca
     9481 ttgaaaatgt cgatcactac agcaaatgga ttgaaagcag tcaatatttt caaagatcca
     9541 aaacagaaaa tgagccaaga taaattttat tttctgatgg atgggatgat tagccgtggg
     9601 ttattcgaaa aagtcgaatg atctgtggat ataaaaagac aactagtttt gaaacggcct
     9661 attgttagat gactaacttt gtaaggacga cgcaaattag ttgtcttttt tatttttaaa
     9721 tttaacacat gttgttattt ttaatatttt gctgatttat tgtttctaag taaagaaatt
     9781 taaattgaaa cgattgttat ttagggaaaa aggtattttt tgaaaaaaga atcccgtata
     9841 atgattgttg tgcaaacgct atacaaaatg aaaaagaaaa gagggatgtt tatggaaaat
     9901 gaacagtcgg tcgttttaac gaattggaag agaaattatt tgttcttttt atcgggacag
     9961 tttttgtcag gaatcactag tatggtcgtc caatatgcga tcatctggta tttgacaaga
    10021 gaaaccggtt cggcgacaat tttgagtttt gctactttac taggaatgat tcccatggtt
    10081 ttactaagcc catttgtagg accgctagtg gatcgctggg ataaaaaagc tcttttgatt
    10141 gtcacggata ttattgtagc gattttcgcg cttattttag cagtagtcgg aacgatttca
    10201 gaatccttcc cgatttggct agtttttgtg tctttgttta tgcgttcggt cgcacaaaca
    10261 ttccaaatgc caacgatcca gtcgattatg ccgacaatcg ttccatcttc tcacataaca
    10321 aggacgaatg gacagttagg aatggtccaa tcagcgaatt tcatcattgc gccagctcta
    10381 ggtgcggctt tgttttcagt cgttcctgtc aattacctta ttttattaga tgtattgggg
    10441 gcagtttttg gtgtcggctt attgattttt gtgaagatcc caaaagtttc tcctgaaata
    10501 ttggaagttc cgcttactat ttttaaagat gcaaaatttg ggctgcagca attgatggat
    10561 aataaagggt tatggtacat aacgatcaat ggtgcatttg tgatgctgtt gttcatgcca
    10621 gcaatcagtc tgtatccatt gatgacgcta gattactttg gtggttccgt tggacaggcg
    10681 ggagcagtgg aagtagtcta cgcagtcgga atgctcttag gcggtgcact gatcagtttt
    10741 ataggaacat ggaaagaccg aatgaaacct attattatag cctacatcat tatgggcctg
    10801 acgatcggtg caagcggatt ggttccaaac gatagtcaag ggttcttgta ctttcttatc
    10861 ttgaatgcag gagcaggttg tgcaacgcca tatttcaata cactgctgat ggcaatgatc
    10921 cagcagagtt atgaatcgaa tgttttaggt cgtgtcttag ggaacttcaa ctctttgatg
    10981 aacctggcag gtccaattgg attattattt gcaggaccat tagctgatcg tttaggggta
    11041 gaaaaaatgt tcttgttttc cggaatcggg attttattat gcggaatcgt tttattttta
    11101 acgtcagctg cccgaaatta tgataaagaa ttacagaaaa aactggtaaa agaacaccat
    11161 gagcaaaaag acgaatagta tggaatggag ttgtgacaga agtcttgtta caactccatt
    11221 tctgatccta atgttcctgt ggcaataagc ctactcaaat gaaaaatatg agtaattatc
    11281 tttatttgtt taatacttga tatagggcag ttttttcacg acctatcgta cttcctcttt
    11341 gcttcataga tttctttgat ttgttgctca gataacggag tgccaaatgg tagtttttcc
    11401 tttaagagtt ctctttccgg tgcgtctacg cctacattga catcgttctc gataggtacg
    11461 ctgtaatgat ggatatgtcc atgaagattg cggatagtgt cgttaggccc gagcaacatc
    11521 ggataatgag tcaagtaata ttgttgatgg tcgaatttaa cgatggcacc tacatcataa
    11581 aaataaaatt tgggacttcc gtcagtaagc ctaggatcat ttttttctaa ataacggaaa
    11641 aaagcgcgac tgtcatggtt ccctttgatc aagtggattg ttccttgcag ttgagtgacc
    11701 agttccaaaa tttctgcatt tgctttttca tattgcggat gcatagcaag gtcgcctaga
    11761 tgataaacat ggtcggtttc ttttattact gcattccagc tagtgatcaa ctgttggttc
    11821 atttcatcca catttgcaaa gagccgcgga gcaaagtcgt tttttcctaa taagcgttca
    11881 tggaaaaagt gcatatcact gataaagtaa ttcatgtatc ttttgcctcc cttttttctt
    11941 agagtaaaca aaatcactta tctgagcaac tcataacatc taaaaagaaa aggttgtaaa
    12001 tgagacgtgc agctttaagc gataagaagc aattttgaaa aatagttttt cacaatttat
    12061 aaaattattc cttattgccg aaaggctggc cctttttatg agtatgccaa ttttttatat
    12121 cgaatgctca aaagatgagt gattttttgg ttgactatca tgtttgtgta ctgaataggt
    12181 ccagtagaac agtattttta cgacttgata attaagggat gaaattttca gaaatatgac
    12241 gtataatgta aaatactgaa aagttgcgac cttcaactcg tagatgtttc agaatctaat
    12301 tgaaatgatg gtgatatcat gacagacaac agcaaaacac gcgttgtcgt agggatgagc
    12361 ggcggtgtcg actcatctgt aacagcactt ttgttaaagg aacaaggata cgacgtgatc
    12421 ggaatattca tgaaaaattg ggatgacacg gacgaaaacg gtgtatgcac ggccacggaa
    12481 gattataagg atgtagcgaa agtagctgct caaatcggga ttccttatta ctctgtgaat
    12541 tttgaaaaag aatattggga tcgtgtattt gaatatttcc tagccgaata ccgcgcagga
    12601 cgtacaccta acccagacgt gatgtgcaac aaagaaatca aattcaaagc attcttggat
    12661 tatgcaatgg atcttggcgc cgattatgta gctactggac attatgcgca agtcactcgc
    12721 gatgaaaacg gcgtggtcca catgcttcgt ggagtagaca ataataaaga tcaaacttac
    12781 ttccttagcc aattatcaca agaacagctt tcaaaaacga tgttccctct aggcggcatg
    12841 gaaaaatcag aagtacgggc aattgctgaa cgcgcaggcc ttgcgactgc taagaaaaaa
    12901 gattcaacgg gaatctgctt tattggtgaa aagaacttca aacaattttt gagcaactat
    12961 ctgccagcaa aaaaaggaaa tatggtcact cttgatggtg aagtcaaagg acaacatgct
    13021 ggcttgatgt attatacgat tggtcaacgt caaggactag ggatcggtgg cggaggagat
    13081 tcgcaagaac cctggtttgt tgtaggaaaa gatctagcga caaacacatt atatgtgggg
    13141 caagggttcc atcatcctgc attgtatgcc acaagcttgg atgcaagtga aatccacttt
    13201 acgacgaatg aaccaatgcc aaaagaattc aagtgtacag cgaaattccg ttatcgtcag
    13261 caagacgttc ctgtgacagt tcgcttattg gatgataatc gtgcagaagt cgtattcgac
    13321 gaacctgtac gtgcaatcac tcctggacaa gcagttgtat tttatgatgg aatggaatgt
    13381 ctgggtggtg gtttgatcga ccacgcatat caagaaacaa aagttttaca atacgtttaa
    13441 gcgatattaa atgaatcaaa acagctatgg ctaacaaaag aaacaccttt tcttttggat
    13501 agcatagctg tttttttgca ataaaattaa tttttattca ttttatactt gaaatacttg
    13561 tgtaaaaagt tcataatatc caataatatg aataaagttg ctttttgtta agcaagagca
    13621 gttgcaaaat agaagggaat cttttgcaag tgatttgttc aattttataa gtgagggtga
    13681 tggaaatgga aaagaaatcg acgggtatta gcaaatgggg actggttgct ctggttgtca
    13741 gttcgtcaat cggaagcggt gtgtttggga tcacaagtga tctggctagt tctgcagctc
    13801 cagggccagc gatcctttca tggataatcg ttgggattgg tattttagct ttggtattat
    13861 cgctgaatca tttaggagaa aagcgtccag atctagatgg tggtattttc ggttacgcgg
    13921 aagcttcatt tggtaaatta ggtggcttca tcagtggttg gggttactgg ttgtctgcgt
    13981 ggctgggaaa tgttgctttt gcaacgatgc tgatgagcgc catgggagaa tttttcccga
    14041 tattcaaagg tggacaaaat gttccttcta ttttatttgc aagtattttt atttggggtc
    14101 tgactttatt agtaaacaat ggagttgaag gagcgagcgt catcaataca atcgtgacga
    14161 tctgtaaatt agtgccatta ttcctatttt taatatttgt atttatcgca tttaaagtga
    14221 atatattcac tgctgatttt tggggtaacg tatctgataa tctagtgaat ggaacagggc
    14281 agcaaggaac gatctggttg cagattaaag gttgtttgat ggttatgatg tgggtctttg
    14341 tagggatcga aggcgcaagt gttttagcca accgcgcgaa gaaacgttca gaagcccagc
    14401 aagcatcgat cattggtctg ctttgtttat tgtttatcta tatccttgct tcgatcttgc
    14461 catacggcgt cttatctcaa gaagagttag caactatttc acaacctgct atggcaaata
    14521 ttttaaaagg aatcgtggga aattggggag cagcgctgat caatatcggc ttgattattt
    14581 cgatcattgg ttcttggttg tcttggacga tgctcccagc agaaacaacg atgttgatgg
    14641 ctcgagacgg tctgttgccg aaaggttggg gaaaaacgaa taaaaaaaat gccccaactt
    14701 attcgttagt tgttactgca atcttgacta atctattctt attgactttc ttagttactg
    14761 attatgccta tcaatttgcc tattctttat gtacagcggc aattttgatt tgttatctac
    14821 ttgtcgggat ctatcaagtc cagttttctt taaaagaagg aatcaaaaaa caagtagtga
    14881 tcggtgggat tgctgtagtt tttgaattga tgggcatcat tttagcagga ttttcttatg
    14941 tccttttatg tagtattgcc tatcttcctg gtttttgctt ctactggatc gcttgtcgtg
    15001 aaaccaaaca tcaaattacg ggaaaagaaa aagtaatgat tgggctgatt tgtgtggccg
    15061 cactgctttc aatcgggttg cttgctgtag gctggatcaa gatataaggg ggaaagaaac
    15121 atgaaaatcg caaatttcgg tgtagaagaa tggctaaata aatgggaaaa gaaagcggta
    15181 tatgatattg cacaaagctc gatcgaagca ttgacattag acgaactcgt tggcttagat
    15241 gggactagcg taagtaaatt tttcgaaaaa cgcaaaaacg aaccattgga ctatggctgg
    15301 atcgaaggat cagatgtttt caaacaagaa gtcgcttctc tttatcaaac cgtcgatccg
    15361 gaaaacatct tacaaacgaa tggagcgact ggtgctaatc ttttagctct atacgcgtta
    15421 gtggaagcag gagaccatgt tgtttcgatg cttcctactt atcagcaatt atatgacatc
    15481 cccaaatctt taggtgccgc tgttgacttt gttcatttga aggaagaaga agattggcag
    15541 tttgatttag aacagctaga agccttagtc aaacctgaaa caaaaatcat ctgtttgaat
    15601 agtgcaaaca atcctacagg cacattgcta gatcggaaaa ctttagaaaa aatcgcagaa
    15661 attgctcgta cagtagatgc atatgtattg atagatgaag tatatgcgcc tttaacggat
    15721 aaaggtgaat tcttgtcgat cgtagatgtc tatgaaaaag gtatagcaac caattcccta
    15781 tccaaaacct actccattcc aggtattcgt attggctgga cagctactgg acctgagttg
    15841 gcagaagttt ttcgaaagta tcgagactac acgatgattt gcggcggagt cctgtcagat
    15901 gatcttgctg tacatgcatt gaagaataaa gaaaaaatat tggcgcgaaa caaaaagatc
    15961 attacagaga accttgtgat tttaaaacaa tgggtagcca atgaaccgaa agttgaactt
    16021 gtcgctccca attacgtctc tacttctttc attaaattag ccatcgaaga agatgaccag
    16081 acattttgta tcaatttatt agaagaaaca ggagtactat tagtaccagg aagtgccttt
    16141 gatttaccga agcatgcccg actaggttac tgttgtaaaa aagaaacctt ggaaaaggga
    16201 ttattcctct tatctgagtt cttgaaaaaa caggcctaaa caaaaacact gcaaggacag
    16261 tctgtgacaa ctgttcaagc agtgtttttc atttacgctt attgtctatg agaaggagag
    16321 tgccctgaag ctatggagac tcctgaagct ttttcttctg cagtttcttg ctgatggatc
    16381 tcttgtgtat tgtgtcgtac aagcaagaag ttgaccaaaa caagaagaga agtcgaaaga
    16441 aagacgccgc gataaccaaa tgcactggaa acactggagc cgatcatagg ccctactacg
    16501 ttaccagttg cttggaaaga ttggttataa ctgaagatcc gtccagcagc ttgatgaggc
    16561 gaatattttg tgatcaatgc ttggacagca gggagcagac aagcatcaga gataccaatc
    16621 aaaaaacgta aggcagctaa ttgccaaaca ttcgtgacga atgccattgg gatataaacc
    16681 agaatcgcaa aacccaaacc aatcgcaagt attctttcac tcccaatctt gtcacctaag
    16741 cgaccaaatc ttggagcggc tatcaaagtg gcaatcccag gtatcgaagc aatgattccg
    16801 ctgatcaacg taacattccc atgtccatgc agcaattggc gaatgtacag actgatgatt
    16861 ggactaatgg aattgttgga tgcttggatg atcatcgttg tgacgaacat accgatgacg
    16921 acgtgtggat atttcaagtc tttgaaaatc tgtttggcag aaaccatgtt ttcttttttc
    16981 actgggacaa atttttcatg gacgaaaaac agactcaaca agaagacgaa aaataagatg
    17041 atccctgtaa tgaaaaaggt agggcgatag ccaaacgctg aagcagagat cccgccaaga
    17101 agcggtccaa gtaatgttcc tgtaacagaa ccagttgcta aggttccaag aacttgacca
    17161 cttttttctt ttggcgtccc agttgcgaca agggcagtgg cattactgat ataaccagaa
    17221 aaaatccctt gaagcagacg caatgcgact aattgataca cactagtcac gcaacccatc
    17281 aaagaaatga cgattgccat tcctaatgaa gcacgcaaca gcatcaattt tcttcctttt
    17341 tggtcggcta attttcccca ccaaggagag ataagcgtag tcaccaagaa agtagatgaa
    17401 aaagtcaatc cgctccaaaa gttcaattgt gacgtactat aattacctaa cgtatctatg
    17461 taaagtgaca taaaaggcat gactaaacta aatccgatac cagccataaa tgttccaaac
    17521 cataagacaa acaagttttg ttcccaagtt tctcttttcc taaataattt ttcctttatt
    17581 cttgcagcca attagactcc ttctttctga aaattttcat ctacagctct tta
//
LOCUS       DAHUVV010000053        17080 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.054, whole genome shotgun sequence.
ACCESSION   DAHUVV010000053 DAHUVV010000000
VERSION     DAHUVV010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.054"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(29..574)
                     /locus_tag="NK767_001758"
     CDS             complement(29..574)
                     /locus_tag="NK767_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCH6694753.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(586..1278)
                     /locus_tag="NK767_001759"
     CDS             complement(586..1278)
                     /locus_tag="NK767_001759"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCH6694754.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1297..1581)
                     /locus_tag="NK767_001760"
     CDS             complement(1297..1581)
                     /locus_tag="NK767_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694755.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1594..2157)
                     /locus_tag="NK767_001761"
     CDS             complement(1594..2157)
                     /locus_tag="NK767_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCH6694756.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2385..3047
                     /locus_tag="NK767_001762"
     CDS             2385..3047
                     /locus_tag="NK767_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCH6694757.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3171..4367
                     /locus_tag="NK767_001763"
     CDS             3171..4367
                     /locus_tag="NK767_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCH6694758.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4468..5844)
                     /locus_tag="NK767_001764"
     CDS             complement(4468..5844)
                     /locus_tag="NK767_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6694759.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5881..6747)
                     /locus_tag="NK767_001765"
     CDS             complement(5881..6747)
                     /locus_tag="NK767_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HCH6694760.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6836..7861)
                     /locus_tag="NK767_001766"
     CDS             complement(6836..7861)
                     /locus_tag="NK767_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCH6694761.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALLEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7879..8553)
                     /locus_tag="NK767_001767"
     CDS             complement(7879..8553)
                     /locus_tag="NK767_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6694762.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8737..9630)
                     /locus_tag="NK767_001768"
     CDS             complement(8737..9630)
                     /locus_tag="NK767_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCH6694763.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9704..11401)
                     /locus_tag="NK767_001769"
     CDS             complement(9704..11401)
                     /locus_tag="NK767_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCH6694764.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11394..12707)
                     /locus_tag="NK767_001770"
     CDS             complement(11394..12707)
                     /locus_tag="NK767_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCH6694765.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12707..16225)
                     /locus_tag="NK767_001771"
     CDS             complement(12707..16225)
                     /locus_tag="NK767_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCH6694766.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16518..16976)
                     /locus_tag="NK767_001772"
     CDS             complement(16518..16976)
                     /locus_tag="NK767_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCH6694767.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4811 a         3842 c         2955 g         5472 t
ORIGIN      
        1 ttttatttta tcaaatcact ttgttttttt ataaaatttc tgcgcctagt gtggactcaa
       61 aatgtctcaa tgcccattca tgtccaacgg gatcgaaact tgccacggca tctttgaaaa
      121 tatggagttt gtatcctaga ttatacgcat cgactgctgt atgcaacaca cagatatctg
      181 tacatacgcc ggtcaaataa atatctgtga tctggcgttc tcgcaaacgg atgtctagat
      241 ctgttccact aaaagcggaa taatgccgtt tgtctatcca ataaacattt tcctcttgct
      301 catagtcttg gtataaactt tttaaagagc cgtacaattc ccgtccaccc gttcctatca
      361 cattatgcgg aggaaataag cggttctccg gatgaaagga atcttttgga tcatgcgcat
      421 caatggcaaa aacgacaaaa tccttttgat caataaactt ctttgtatga gcagtcagct
      481 ttttttcaat tacttgtcct gctgtcccag tagtcagctt tccatcctct gcaacaaaat
      541 catatgtgta gtcaatcgag atcaatgcgt tcatcttagt cctccttata cgagatgttt
      601 gacaaattcg atcaccattt ctgctgatcg tttgcctgcg tcttcgataa attcgtcaaa
      661 gctttgtgtc gccgagtgat ctgctgtgtc gctcattgca cgtacgatca aaaatgggat
      721 attgaactga cgagctgttt gtccaacagc ggctccttcc atttcgcaag ctaatgcttc
      781 agggaaattc gttaagattt ctttgatttt atctggcgag tcgacaaatg tatcacctgt
      841 aacgatcaat ccttcttttg cgttcaaatt cgttgctttt gctgctttaa ccatttcact
      901 gcgcaaatat gtgcttgctt cataatatag aggcattcct ggtaattgtc ctgggttgta
      961 gccaaatcct gttgcatctg catcaaaata agctactttg tctgatataa cgatgtcacc
     1021 tacttgtaag ccttcaccga ttccgccagc tgaacctgta ttgatcacca tgttcacgcc
     1081 gtattgttgg atcaacagac ttgtggtaat agatgctaag acttttccta taccggaacg
     1141 tacgacgatc acttcgtggt tacctaatga acctgaaata aataaagcgc ctgcgcgttc
     1201 ccatgataat ggttcgctta ggttctctct caaaattttt acttcttctt ccattgcgcc
     1261 gataataccg attttcatct ttttaattcc ttctttctat ataaatgcga ttgctaaaaa
     1321 aaccacacat aacattagga taacaataat aagagatttc atcaaaaaag agttccattt
     1381 ggtcgtcttt tcccgttcgc tgatccttgt tttcgtgatt ggtttattct ttttgctttc
     1441 tttacgataa aagctggcaa tctttttttc ttcttgctga taagctgttt ctgcttttcg
     1501 ctgttttttt aatgactcgc ttgcttgtgc ttgttgtcgc ttgcgaagtt cgcttcgcgt
     1561 aataagcggt cctttggcca taagccgatc cctctacttt cctttggtca atagttccca
     1621 gtattgaatc gcataaatcg tctttgcatc acaaatcaac tgatctttca tcgcttgttg
     1681 cgcctcttca agagttagat gatacaactc cagtacttcg tcttcatctt gtgctaaagg
     1741 attctcgacc ttctctaccc cttgtgcatg ataaatatga aggacttcat ttgcaaaacc
     1801 tggtgaaaga tacatcgaag taagatggct cagcgacttc gcacgatagc cagtttcttc
     1861 ttctaattct ctagctgctg tcatttcggg attttgtcct tctcctggat cgatttttcc
     1921 agctggaatc tccaaaatca ctttttctaa aggttttcga aattgtttga caagaagcag
     1981 acgatcttgt tcgtcaaaag caatgatccc gacaccgcca ggatgaaata ccagttctct
     2041 cttcgaagtt cctccatctg gcaaacgaac ttcgtctaca gcaacatcaa taatttttcc
     2101 gctatagatt tctttgcgac tgattgtttt ttcttcaaat tgctcattgt tcagcatatc
     2161 ttctcctgct ttctttgtaa tcataactgc ttttttttca tttttcaatt tttctcaaaa
     2221 actccttatt agtttaccat agataaacct tagattttct ctaaatatag aaaatccttc
     2281 tttagtccga tactctttat agtagtccgt atgctaaaat aagtatgtaa tcgaataacg
     2341 aaaagaattc aatactcaac gataaatgac atggagggtt tattatgata aaacgaaatt
     2401 ggccgtggct tctagcattg gtcttgctat ttctctttgt ccgaaacaat agtttttctt
     2461 ttgttctagg acttttattg ataggtgtag ttttagtttt gtggggactc tttggttcgc
     2521 gcaggaaaaa gggaaaacaa gaagaaattc ctgcactgac tgacgaactt gaaactcact
     2581 atgaaaaaag cggtatgaca gcgaatgaaa tttcattttt tcgacaaaca atgaatcaaa
     2641 caaaaaatga gatcaatcaa ttgcaaatga atatgcagca gacagccaaa cttaaagcaa
     2701 tcgatctccg ccatgatcca gtcaaagcag cgaaagcttt attcaaagaa ttggtgaaag
     2761 aaccaaaccg actgcatgaa gcaagtcagt ttctttatac gcatctgccg aatctagttg
     2821 atttgacaaa taagtataat gagatcaatg accatgagat caaaaacaaa cagacctatg
     2881 aaaaactaga agaaagcgcg cagatcatcg accagctgtc agctttaatc gcacaagatt
     2941 atcaaaaatt cgttgctgag gatctagatg atatcgacgt agagatatct gttgcgaaac
     3001 agagcttaaa gcgtgataat aaatatgaac cagaacaaaa ggattaactc ttttgttcat
     3061 aaaagaagac tcggaacatt cagatgatgt ctggctgttt cgagtacttt ttctaaagta
     3121 gagacattca tgtaagaatc gagaacagat tcaacaagga ggaatttttt atggatcata
     3181 aaccgactga aacaaaagat gttacaccag ttaacgatac acttgaagat ttattgaaca
     3241 acccgttttc aactccaaca gatacgctga ccacttcaca acaagcggag attgatcagc
     3301 tccaagagca acagacagct gctcgtttaa ttgacaaact accggcggaa cgccaagaac
     3361 aagctaaaca gctagcagca aaaatcgatg ctaatgatgc acagtctgtc atcagctacg
     3421 gatcggctgc ccaagcaaaa ttaagtgaat tctcacaatc gatgttgaat catgtgcaag
     3481 cacaagatat tggtcctgta ggagattcct taactgaact gatgtatcgt ctccaagaag
     3541 ccaatcctga cgaactgcgt gccggagaag ggaacatctt ccagcgtgtc ttcgggaagg
     3601 tcaaacaatc gatctatgaa gtgacagcaa aatatcaaaa gattggtgca caaattgaca
     3661 agatttcagt gaaattagat aaagaaaaag acggtctgct aaaagataat ctgatgttag
     3721 aacagcttta ccagaaaaac aaagactact ttgatgcact gaatatttat atcgctgctg
     3781 gagaattgaa aatggaagaa cttcaaacaa cgatcattcc agaagcgatg aaaagagcag
     3841 aagaaactgg cgatcagatg gatgttcaga ttgccaatga ttacacgcaa ttcctagatc
     3901 gtctggataa acgtacgcat gatcttcgct tagctagaca gatcacgatc cagcaagcac
     3961 cacaaatccg tttgatccaa aacacaaatc aggccttggc tgaaaagatc caagcatcta
     4021 tcgctacagc cattccgctg tggaaaaacc aagtcgtgat cgcattgacc ttgcttcgtc
     4081 aaaaagatgc agtaactgct caacgccaag tctctgaaac aacaaacgac ttattgaaga
     4141 aaaattcaga aatgctgaaa atctcagcca tcgaaacagc gaaagaaaat gaacgtggaa
     4201 tcgtggatat cgagaccctt caaaccactc aaaatgattt gatcgaaaca atccaagaga
     4261 cattgcgtat ccaaaaagaa ggaaaagaaa aacgtcgtca tgcagaagta gagcttggac
     4321 acatggaaga agatctgaaa caaaaactgt tggatttaac ccaatagatc acctttcgct
     4381 tgttactaaa acaaacgccc aacaacagtt tattacaact gttgatgggc gtttttctta
     4441 tctttcttct aacttctcca ttggagtcta tttctcattc atcttgctgt gtccatagcc
     4501 atagccaaaa taaatgatca gtccaataac cattacgata ccgaacacga tccatgtcac
     4561 agtttgcaga cgaagcatca atacaataca agtcaagatg gagacgattg gcaatagcgg
     4621 aacgaatggc actttgaatt ctccagcttt tggtttgcca aacatttttc tcaacttcag
     4681 taagcctaga ctaacaagca tcaaataggc gagcgccatg atattcgtta gttccgctaa
     4741 tacattcaat gggaaaaatc cggcaaagaa tacagctgcc acaccagcgg cataagtcgc
     4801 ttttttcggt gtacgatgct tttgatcgat ttctgtcata aacttaggaa gtaagccatc
     4861 cttgctgaca gcaaacagta aacgagcaag tgaatacatc atcgagatcg ttaccgtcag
     4921 tagcgttaaa attgccccta cagcgatcac cgcgcccact gttccatgtc ctacatacct
     4981 cattgcaaaa gccactgggt cattgactcc tagttcagta tacggaacga ttcctgtcaa
     5041 gatcagcgtc acgatgatgt aaaggacagt agcaatcagt atcgaaccaa tgattcctct
     5101 tggcacatct ttttgcgggt ttttgacttc ctcggctgcc attgcaacgg catcaaatcc
     5161 caagaaagca aaaaacacaa gagctgcacc atctagcaca ccggaaaaac caaaaggcat
     5221 gaatggctgc cagttatcag gtttaacgaa aaatattcct acaatgataa acaacgcgat
     5281 gataccaaat ttgacatata ccatggcatt attcaaacgt aatgctttct tggcttcaag
     5341 cgacacgatg taagtgacga atatcactac gagcaccgca atcaagtcaa tgtatgtacc
     5401 attttccgga ttgtacccgc cagttaatgc ttgtggaagt tcggccccta aacttttcaa
     5461 aaaaccttgc atatagccag accatccgga agcaacagag gaaaccgcta gtaaaaattc
     5521 cccgatcaac aaccaccctg tcagccaggc agcaaattca ccaaatacaa catacagata
     5581 ggcatatggc ccaccaatca ctggcactcg cgaagcaaat tctgcgaaac ttagtccaga
     5641 caaaatgaca acgattgcag ctaagacaaa tgaaagtgac agtgctggac cagcattttg
     5701 attggccgca acccctgtca ccacaaatat tcccgtaccg atgatcgcac caattccaag
     5761 catgatcaaa tcagacgttt ttagttcttt tttcatctca ctcgtatgat tgagattgat
     5821 ctctttctta cgaaacaaat ccattgaagt tcctccccat tttaggttgg aaaacagcga
     5881 ttaaactgtt ttcatatacc ctaaaaggat accacctaaa atgactaaaa ggcaaccaac
     5941 aataacataa atgagctctt ttttcgtttt tctttcgcca aggatgaaaa tcccacctaa
     6001 agtagagata atgatcccca tttgtgaaag tgagaagcca acagctaaac caagtgattt
     6061 gactgttaga agcatgcaga tgtttcctaa gccccataat aatccacaga tcatgttttt
     6121 ccacacaaac tgatcgactt taactttacg gaaagcaaag aagcttgcgc ctaagatcat
     6181 tccgatactt tgaggtaaga taattgacat cgcatccaaa ttcgcccagt tgacgatgat
     6241 ggtatataca ccataaccaa tcgtagagaa gatcaatgct ctaaaaccct taccaaaatc
     6301 aagcatttta ttatctgtat ctatttttcc ttcgtcatct tgtcgggcag tcaaatacgc
     6361 accgcttaca agaagagcta aagcaataat accgtataca taatctttcg tttgtgtcca
     6421 ttcatggaag aaaacggctc ccgcaatcgt attcaaaatt aattgaaaac cagtagacaa
     6481 tggtagccct acagaaacac ccatgtactt cattccatgg aactgtccat tttgtccaac
     6541 actccaagct aagccggata aaaatccgat caagaaaatc caaggggtga ttacaggact
     6601 aacaacgaaa aagacaacta aagaaaacag aaatgcaccg atcgtcattc ctagtgtttg
     6661 ttgattggca tcgccaccaa ttttaccact tactaaacca atactacccc atgccaacat
     6721 agggactaat gctattaaaa tttccataac caatctcctt ttatttcaca tgataagaaa
     6781 cttatcagcg aaaacgttaa caacacaagc actttttttc gtttctaacg attttctaac
     6841 gtttttcatt ataaaaattt aagattggga aaaataaagt cattgttgtc ccttttcctt
     6901 cagttgattc agccgtcaaa tggataccta atttttcggc cagatttttt gctaaataaa
     6961 gtcctaatcc tgttgaatgt gttttgctaa gtcgaccatt tctcccagta aatcccttat
     7021 caaagatccg tttcaagtct tccgctggga taccgatccc tgaatcttta actgaaaggc
     7081 tgactccttg cgcttccttt tctatgctca caatgatgtt tccgtgatcc ggcgtatatt
     7141 tgatggcatt actaagcaat tggttgaaga taaaagcgat ccatttagca tcggtcaaaa
     7201 cagtatgatc ctcaccaacg atctcgtagc gaatattttt ttggataaaa taatttgcct
     7261 gactgcgtat cacaggctga atgattcctt ttagtccata ttcttggatg aggtagtccc
     7321 gcgagaaact gtcgagtctt gcataataca aaacttgttc tacgtattca tcgatttttg
     7381 ataattcatt ttctagtaat gtatactttt gatcgtcaat atcgaactcg atcgaacgaa
     7441 ctagcaattt agaagcggaa agtggcactt tgatttcatg gacccatgag tcaatataat
     7501 ctttttggtc ttgttgatta ctgattgctg attgcattac ttcttggtgt tcacggatca
     7561 gctgattgat atattcttct accagttttt cttcttctgt cctcccacca ctcaaataat
     7621 tttgcaaagg ggaagttttt tgtatgtcgg ctaattttcc ccaccatctt ctcttgctca
     7681 agtaaccacc cagcagataa aaaatcagaa agacgccctc aagcaaagct aaataagcaa
     7741 ttgtgctcaa atcaactttg atgtttggcg ccagccacat cacaaagatc gtcaatgcaa
     7801 taaaaaacag ccatcccaag atcaatgtcc actgatcttt caaatattta aaaaaattca
     7861 tcttaaccgc tcatcctttc agggaacgat atatccctgt cctacttttg tttcgatata
     7921 tccttggata cctgcctgtt cgatttttcg gcgtaaccga ttgatgttga ctgtcaatgt
     7981 attgtcatct acaaatcgtt cgtcatccca cagtgctcgc agcaattttt cccgacttaa
     8041 gatctttcca ttgtgcttca tcaaaataaa taatagacga tattcatttt tgctcaaatc
     8101 aatgatctct ccgttgattt ccatacttcc gttgtcaaca ttcaacgtaa tgccattgtg
     8161 gctcatcatt tcactagata attctgtgta gttgtaagaa cggcgaagta atgcattgat
     8221 ttttgcaatc agtacatcga tttggaaatg ttttgtcaca aaatcatctg cccccatgtt
     8281 catagccatg atcatatcca tattcgtatt cctgctggag ataaaaatga tcggtacttt
     8341 cgatacttca cgtatttttt gacaccaata atagccatcc aaaacgggaa gattaatatc
     8401 tagtaataca agctgtggtt cttctctttc aaagtctgag aacacttgat tgaaatcggt
     8461 cgttccaaag gtttcaaatt gccatttctc aagttcctcc atgatcaatt gtcgaatgat
     8521 ctcttcgtct tcgactacca tgatttttgc cataaaaaat ccctccatct ttcttcttgc
     8581 tactacaata tcaaagaagc ccttgtgaaa caagttggct tacctggaaa atgtcatttt
     8641 agcaaacgtt aaaaaaaact ggaaatatgc gatttaatcg cgtttgtctc ttccaagcag
     8701 cttaaattta ttatgttatt cttaataatt ggtctattat ctgttatttt tctttcgttg
     8761 tttttgtctt ttaaattttc gataacacca ataaccgaaa agcgttagca ctagcaatat
     8821 acctagaagg agtataagta atcgataaag atggtatttt tttgtctgtt ctatctttga
     8881 agagacttct tctgtaaagg gaactcgtct tcctgtcact agtaatcggt gagtgttcac
     8941 ggtgtaaggt gtacaagtga taagtgtgac tagatcctgc ttttctctaa ttgctaatgt
     9001 atttgttttt gttggaagga caacaatttt ctcaacgact tcataagcga gcatccgacc
     9061 atacacttca agatagaatc ggtcatcttg ctccatttct tcaagatccg taaagagttt
     9121 tttctcagca ataccgctat gggcggtaat cacggaatgt gtactctctc ctccagttgg
     9181 aaaagaggtg ccttgcagga ctgtcgctcc tctatctaac aaaagatcat tcgtttcatc
     9241 aaataaaggc aagctaacat tgattttcgg aatatagatg gctccgatca tatgttgttc
     9301 gtaatattct tttcctggat tttggacatt tctaaccgct tgctcaaaag gatctttgac
     9361 ttgtcccatt cctgggatta ctgatgtatg agcaagcgtc tgatttttct tttcatgcac
     9421 tgctaattgt tgctcatgtt tttcttcttt atcgcgttca agctgctttt gataattctc
     9481 gatccttctt tgatccaaat aattgctgag agcatctgct aaaaatggat atgtaaaaac
     9541 tgcagctccg gcaagaaaca tcactgccat aaataattgt aataaaagac tgatttgggt
     9601 ttgaggtcta ttacctattt tctttttcat tttcttcacc ttcttattta aaaaaactgc
     9661 agcaaccttt ataaaaccga ttgctgcagc ttatttttgt tgcttatact tcagacttca
     9721 ttttagattt acggtaccag ctgtacgctc caatcatcaa acttattccg attagtaaga
     9781 atgccaagag cccgttccca ccagtagcag gaagtgaccc gcgtttcata ttcggtactc
     9841 tttctggccc tggattttct actcctacga tctgaatttc ttcattatag cttccataag
     9901 agatgataaa ttcagtaaag gctgcctcac ctgttggaag tacataacct tctggcgcca
     9961 tggtctcttt catttgatag gttccataat ctaacccttg aacctcaaag tatccatttc
    10021 catctgcgac aaattctgtt gcttgtccgc ttgttgccca ctcaattcta tctgctcctg
    10081 attcataaac agctaaaggc gactcccctt tataaaatat tgcgtattca gaacggtctt
    10141 cattccacaa ttcgaatctt gcatctttaa gcaattcact gcttgatgca tcatattttt
    10201 caaactttct tccacctgtt gtgactttct ctggcggctc tactccttct tcataatcaa
    10261 attcatcatt tcgtccgatt tcaataaccg ctgtgttgtt atggaaatca tccgcttcta
    10321 gttctgtgga aaccttcaaa ttatacgtga tcgttaaatc tcctccacgt gcatctgcta
    10381 atctacctaa agtatctact ttggctactc cattttcttc tgtcaaaatc gtcacgacaa
    10441 acccgttgct tactacttct actatataat cttgtccttt tgttaaaatc gtctcgccgg
    10501 ctcgcacttc tatttcctga tcataataag ttagaccaac tgtaggcgta tctgtgattt
    10561 tgtaattttt gataacagcg cttccgtttt caaggacaga atcgatgaaa taaggaatag
    10621 gtgctgtgat cgtgtaagaa gccgtttttc ctctttctag atcaatataa gaaatcgttt
    10681 cttgttctcc agcttcatta ataatcgtta cttgtctaag gtcgctttcc tctacattca
    10741 tctgtttatc tgcttctctg atttcgtttt tcggatacaa atgcacatct gtattgtatg
    10801 tgtctttcca tgtagaacca tcccacacgc catccaccat atcaggcatc atcactggca
    10861 tcataacaac cattggagac gcgattctgg aaatatgagc tggcgaataa gtttctaaga
    10921 aaagatagac tttatctcta gaagaaggtg cttcttccgt cacttggacg gtagagaata
    10981 ccgcttctcc agcttcattc gttgtttgtt tgtcaatact tgtcagtcga tattttaaaa
    11041 accaactttc tgtgtgccgt ttttgaatcc attcaatcgc ttcggcagaa ttaagaccgt
    11101 cttctcgttc cggtgttaaa ggattatctc gaatcaaatc gtaatatact tccgttacgt
    11161 caaacaaatc aaaatcgact cctggaatat tttcttcacc aaaatccgta gaaaccaatc
    11221 cgctattcgg tcgttcttcc ggaacagatg agaaaccttt tttgtgtaat gtaatcgtca
    11281 cttcacttgg tttttgtgcg acttcttcgg ctttgactag ttttccgttc ccgaaaatcc
    11341 ctattcccgc agtaacgagt aacagtacac tcattcccgt gacaatcatt tttttattca
    11401 tttttttctc cccgttcttt ttttattaat aaaacagcac ctaacaataa aagaattcct
    11461 agtaaagaaa tcaacgttct tgcttcattc gtttgcggga atacggacca ttttcctgat
    11521 tgacctggat tgcttggacc actcggctct ttcggtttgt ccgttgtttc ttttacggtc
    11581 tcgttataaa cacgtggtgt tgcggattca taggcagaga ctggaacttt cccggattct
    11641 aattcagcca tctcctgtcc gttaactact acatattgtg gttggccttt tgcatccacc
    11701 caaaattcag ggatgatgac ctcgattctc ttacttgctt catccatctc gtaaccttcc
    11761 acacctttta attcttcaaa ataataggtg ccagaaggca agaaacgttg tcctgtcgta
    11821 acaaggccct ctttatccga aatgaatttg cttacatttt tgtcattcaa aggatcattg
    11881 tctgctggtt tgatccattg atttttcaag tcattagcag gagccatatc taaataatat
    11941 ttcccccctt ctatcatctg atatagagca aaaacagcac cttctaatgg ttttcctttt
    12001 tcagttgttc ctctttgacg tccatacttg aagaagtatg gatctcttag ataaccgaca
    12061 tttttaggat agatatgaat ctcttgcagt tcctcggttg attctgtcgg gtgataaatt
    12121 ggcaaaatag cagcaattgg gatagctgtc tgatcgatat caacatccaa tccaacttct
    12181 ggatcaatcg attcttcgaa aatcaaataa actgcatccc gatcttggtt tttcttaggt
    12241 gctttgatac gtgcaatccc gtctcctaat ccgccaaccg tatctttccc agttgttacc
    12301 gaagtcccta tttggacaag cctttcttct gtgattagct gacggatatt gtttctgttc
    12361 gtttcagcga tttgctggac gaattcttta ggagtcatgt tttcagcagc ttttttttca
    12421 tgataataat cggtcatgtc gtaaatggca aatgtcgctc catttagtgg caccgtctgt
    12481 gaaatgatat ctgctgtttc ccctgcttcc tcagagatag ccaagccatc attttcatgg
    12541 tattcaaatt gatcgttata atcgatatct cgaatcatcc gtttatgcaa cacgaattcc
    12601 acgacatctt cccctgattc ttctgccgac atcgcatgtg gaagaaggac tgctgcaagc
    12661 ggtagtaaaa caagtaattg aagcatcaaa cacaacagct ttttcattac cttgctccct
    12721 cctttttaga ttgtctgaca aacaaaccat aggctgctcc tgcaagcgcc atgaagaatg
    12781 cagcaagcag gatatagaat aatcgtccgg atcctcctgt agcaggcaac ggattttcag
    12841 gatcgttccc gacactaagc tgtaatctga ttcgatttgt ctccaaaagt tcatattcta
    12901 actcttgctg tagatcaggc gtctctgctt cttcgtttgg ataatacgaa atctcaactt
    12961 ttagtgtacc cgcttctttt cctggaacta cccgatcatt ttctatgtca ggttcttctc
    13021 gataactaat ttctaaaata aagtaacctg gaagttttct atagctgtca ggtgacttca
    13081 cttccttgat ccgatagacc ccttccaaca agtctgaagc attgaatagg gaagaatcgt
    13141 ttggatctgg tgccaagcct ccctcgatga tttcatactc tcctgcacta ttttgtttct
    13201 caattgaaaa ttctgcgcct tcaagacgtc ggctattcat actgtcaatt ttcaaaagtt
    13261 ccaaatcgat tggcgctagt tcatcttcta aggttacttg tagtacacca tcgatgattt
    13321 catagccttc cggcaaggga gcagtttcgt cttcgatgac cgtatcttca atgataaact
    13381 taccttcttc attcagtgca aactggaaca cttgatcaga tagccgatac ccatttggtg
    13441 cgacagattc cgagacctca tattcaccaa tagacagatg cagaggttgg tcgtaattta
    13501 aatcgacata cttttctact cctacggcac gagaatagaa gtatccgatc ccaggcaaac
    13561 gtttcgattc ttctggttct aacggattac cagtgtatgc tcgatacagc gggaaggaag
    13621 aatcccccac tttcctgata tcgaaacctg caccatctaa tgtatggcct tcatggtcta
    13681 attttctaac tcggatctcc cgataatcaa acgtattgta agcagtgaat gctacccata
    13741 aacgatcctc ttcatctgtt gtatacgtga gatattgcca attttcttca cctgtttgct
    13801 tgagttggat agtccctata gcattatcgt aaagtcgcaa gatccatgtg tcattttcat
    13861 caggttcttt gaaaccttct gggttctggc tctcaatgat tttgtattct gcatagttgg
    13921 tagtttcttc actatcctta taaacaaatg aatctggctc tccgattgga tgaataactt
    13981 tctcaccatc cacgattaca tatttcccgt cttcatctac tgaatagaac gtgagtctcc
    14041 ctttctcttc ctctacttcg ttcacaccag aaagcccttt cgctactgga tatccgattt
    14101 ccagtccgtt accatctgtt tcataaagtt caaactctgc gccattgatt ggcatattac
    14161 tgccttcaat tagtttattg atattcatgt ggattggctt gaattcattt cgcatttcca
    14221 gtttgacata caactcatca tactctgtgc cttctatgtt aggctcttgt acaacagaaa
    14281 gcttgtgata ttctccttca taataggaat tcggatcttt accttccggt ccgccgactc
    14341 tcgaaccaga tgtatattca tctacaagct cgtagtgacc taacatctca tcttcgttgg
    14401 ctgcatattt tttgatttgg aattgatacg tctcatctac taactgataa cgtggtggtc
    14461 cagcgatctc tctgatccaa aatatgtcgc tgactgctcc agtttcatct cctaaaatcg
    14521 gcatcttcgt ttcaaagatc gctattccgt ccgtggtagt tttacttgtt tcgattggtt
    14581 cggcttcccc actctctgga ttttggtcat ataatgcaaa ttcgactcca gccaacggct
    14641 cgccattttg acccttcttg ctgacttgga aattacggcc tttatgttcg taacgaatgg
    14701 aagaagatgg gaaatacatc cgtccagtag tgatatcatt ctcataatcc tccaggttct
    14761 gaatataggc catattgttc actgtgcaaa aatttccgtc ttgagctccc gcatcaagtt
    14821 ttacttggta cttgatcctg accatttccc cttcacctaa agaaatcgga gaaaatttat
    14881 aagcttggtt ttcccgtccg acatacgttg tcggagtcgc cactacttct ggtggggctt
    14941 gtccatgacg ttcttcatcc catatttctg gctcttggta cttcatgccg ttttctcctg
    15001 gatctagatg gtaaaactga atatctgccg atctgccagc tccaaatggt gagacattaa
    15061 aaccagtttc atcataaagt gtggcttgct ggatcgttct cttgaaggta gaagagatat
    15121 cctccaaggc tgtaacaatg tcattcttgt tgttcgcatt gtaatatcgc ggtgttacgc
    15181 catcttcttc taaatcagca atatttttca atacatttcg tccaatagca tctagtcgtt
    15241 gtctcccgct aacgtttcca tcgattccta aaccaatgga gaagatactt ggcgggttct
    15301 cttgatactt gtttttcagc caatgatgat aagcaactgt gttgatcgca gatgactttt
    15361 cttgcgaagt aggactgcca ttcgtattgt tattagaaga accatcacca acccagtgag
    15421 tcgctttatt atctccttct tttacctctc cataacgata gtgtacattt acttcatctt
    15481 ctggacggtt aaagtcagat gcataggtaa ctggatgagt aaaccctgca ttagaagtat
    15541 tcccagaaaa ctcctcaaaa tttctgaaca aaacgtcatt atcttcagca atcttatctt
    15601 ccatcacagt ccagttgctc caagctccat tagtgctcgt acgataactt gactgaatcg
    15661 gtgcataact gaaggtcggt gtcccatctc ccacaacgat taggattttt tctggatttc
    15721 tattttctcc gccattgtct ttatacaatg tttcatagcc atttttaagc cctagtgtaa
    15781 gcggagttcc tccgattggt gtacgattta atgctgtatt ctcacttccc gtcaaacgat
    15841 tgatatcatt tgtcaatgga aagatgtccg aactgatttg ctcatggtta ttgatagctg
    15901 tactattatg gtaaaaattc accataccga ttcgaatatc cagactcggg tcagagagca
    15961 gatctttact catttcgttt actgcctcaa tcaacgcagc atttttagtt tgactattgg
    16021 tcccagcggt taattcactc atactcgctg acttatctag aacaaaaacg atatcgattg
    16081 gactgatttc ctttttctcg cccccgatga catctagata gacattgtaa agggttgttg
    16141 catcttcagg atgtagatca gatgctgtgc tgcttggcag aacaggttct acccattttt
    16201 tgatgtacgc cccgttataa tccaagtaac tgttttcaaa attccgttca tttccccaaa
    16261 gctgaacggt ttgagctgga acgtttctat cttcttcgcc tgcagttgat ccattattag
    16321 tagcatagtc gtaatttcgc cagttcgctc ccccgtttgt ataatcacta tcatcgatca
    16381 agttctctcc gtcatatggc gagcctttgt ctccttgata gcgttcattg ctttgtgtcc
    16441 aatttcctaa cacttttgta tagtcattcg ttgtaaactt catctattct ttcatcaacc
    16501 accacttgaa gaggtagtta cccttcgttc acatcgggga tcttttctgt ttcaaaaatc
    16561 aatgtgccgg tttctgcttc ttttgaataa acttttccga cataccattc ttgaccatcg
    16621 atcgtctcga cttttaggtc ttctccatct tcatcatagg aggatagacc tgttccacga
    16681 acgtttttta cggtaccaat cccggcggct gccgcatcga ttgctaaacg tattctacct
    16741 tcattggcac tttccttttt ttcaaaagcc agatgccatt caagggtttc ttctttttca
    16801 atatacttcc cttcaatttt tgcatagtcc tcatctaaga atacctcatc ctcctttgtt
    16861 tctgttgact cagcagctac atccagggtc gtcatatgat agactgactg gaaatatggt
    16921 ccaaatagca aactgacagt tactaactgc aaaagcttca attttaaccc tttcacatcc
    16981 ttatactcct ttcaaaaata tttgtcttct ttcacaacaa tcaacaaaac aatgcccatc
    17041 taattataat ttaatttaat tttaatttta aatcaaatga
//
LOCUS       DAHUVV010000054        16820 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.055, whole genome shotgun sequence.
ACCESSION   DAHUVV010000054 DAHUVV010000000
VERSION     DAHUVV010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.055"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..456)
                     /locus_tag="NK767_001773"
     CDS             complement(<1..456)
                     /locus_tag="NK767_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCH6694768.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
     gene            complement(561..1424)
                     /locus_tag="NK767_001774"
     CDS             complement(561..1424)
                     /locus_tag="NK767_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCH6694769.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1620..3719)
                     /locus_tag="NK767_001775"
     CDS             complement(1620..3719)
                     /locus_tag="NK767_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCH6694770.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="NK767_001776"
     CDS             complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="NK767_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCH6694771.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5072..6913)
                     /locus_tag="NK767_001777"
     CDS             complement(5072..6913)
                     /locus_tag="NK767_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCH6694772.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7095..9410)
                     /locus_tag="NK767_001778"
     CDS             complement(7095..9410)
                     /locus_tag="NK767_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCH6694773.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9595..10455)
                     /locus_tag="NK767_001779"
     CDS             complement(9595..10455)
                     /locus_tag="NK767_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCH6694774.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLCGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10514..11035)
                     /locus_tag="NK767_001780"
     CDS             complement(10514..11035)
                     /locus_tag="NK767_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HCH6694775.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11313..12074
                     /locus_tag="NK767_001781"
     CDS             11313..12074
                     /locus_tag="NK767_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCH6694776.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12100..14691
                     /gene="mprF"
                     /locus_tag="NK767_001782"
     CDS             12100..14691
                     /gene="mprF"
                     /locus_tag="NK767_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCH6694777.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="NK767_001783"
     CDS             complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="NK767_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCH6694778.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16434..16790
                     /locus_tag="NK767_001784"
     CDS             16434..16790
                     /locus_tag="NK767_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCH6694779.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4617 a         3491 c         2946 g         5766 t
ORIGIN      
        1 tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat gatcttttac
       61 taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat cctcttctac
      121 ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt tggcgccttc
      181 ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga caattttcaa
      241 ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg gactcttttt
      301 tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt ccaaaagcac
      361 aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta gctgctgttt
      421 tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa caatttataa
      481 taattacaaa taaaaaaaga tataacacta tgttaattaa aattattata agctagttaa
      541 gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac accgttttct
      601 tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc tcctttgctg
      661 ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt gatttgctga
      721 tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat agcttcatat
      781 gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat cactcgacct
      841 tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat aaacccaaga
      901 tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc tgttcctatc
      961 acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa tattttttca
     1021 tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat catcaagcga
     1081 tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga tacgacaaag
     1141 tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt agcagtaacg
     1201 attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat ccgattttca
     1261 tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg ttcaccttct
     1321 gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt ttcattttca
     1381 tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt ctccctctat
     1441 agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat ctacttgaat
     1501 agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt gtcatactcc
     1561 cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt tgcctaaaat
     1621 taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac gatgtaaaat
     1681 ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt ggcatataga
     1741 ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc tccttcaacg
     1801 atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg tgcccacgtc
     1861 catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat tggctgattc
     1921 gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc
     1981 gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt tgcttgttct
     2041 tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt tttctcttta
     2101 tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga tagatctgta
     2161 tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc
     2221 actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg caattcagca
     2281 tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc
     2341 gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc tagatgatca
     2401 tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg gatcttagat
     2461 tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt aagactggat
     2521 aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat aaattgatgg
     2581 tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg ttgctgtctt
     2641 tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata agcccagctt
     2701 cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt tgattttttc
     2761 ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt tttttcagta
     2821 tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg gttctcttct
     2881 ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt ttcagcatcg
     2941 gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc gagtatttct
     3001 tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt ttcagtaaat
     3061 acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt ttctttacct
     3121 gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg ctctactttg
     3181 ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg ctcttctttt
     3241 gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc ctcattatcg
     3301 acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt ttcttcttga
     3361 ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc atttttctct
     3421 atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc tttttcgaat
     3481 aattccatta cagattctag aggtaaatct ttcaattccg accattcgat tggatcgttt
     3541 aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc tgcatctgta
     3601 tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg atgaccgcag
     3661 tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca ttttttcatc
     3721 acttagcgat cggctccttt actaggttta cgctaccatt taatctatca tattctatac
     3781 gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat ttccttccta
     3841 tagaaaaaaa agcttgagac tgcgtagtct caagcttttt ttcttttaaa ataaaccaaa
     3901 cagttttact gatttctctt ctggtcaata gtccacgctt attctaagaa atcttttaat
     3961 tgttttgaac gagaaggatg gcgcaatttt cttaatgctt tagcctcgat ctgacgaata
     4021 cgttcccgag taacaccaaa tactttccct acttcttcca atgttctcgt ccggccatca
     4081 tctaaaccaa aacgtaagcg aaggacattc tcttcccgat cagtcaatgt atctagtaca
     4141 tcttccaatt gttcttttag taactcataa gctgcgtgtt ctgctggact tgttgcttcc
     4201 tggtcttcta tgaaatcacc taagtgagag tcatcttctt caccgattgg cgtttcaagg
     4261 gaaacaggtt cttgcgcgat tttcaagatt tctcggactt tttctgttgg aagatccatt
     4321 tccgcaccga tttcttctgg cgtaggttct cttccaaggt cttgcagcaa ttgacgttgg
     4381 atacgtatca atttattaat tgtttcaacc atatgaactg gaatacggat cgtacgtgct
     4441 tggtcagcga ttgctcgagt gatggcttga cgaatccacc atgtagcata ggttgaaaac
     4501 ttaaatcctt tgcggtagtc aaatttttca actgccttca tcagtcccat gtttccttct
     4561 tggatcaagt caaggaactg catgcctcgt cctacataac gtttagcgat acttactact
     4621 aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt ggtcgccttc ttcgattttc
     4681 aatgctaacg ctacttcttc ttccgcagtt aaaagggaga cacggccaat ttctttcaga
     4741 tacatacgaa ccgggtcatt gatttttaca ccggttggcg cagaaagatc ttctgttttt
     4801 gcttgttcag cttttttctc agcactcttc aagctgtgaa ttgatggatc accattttca
     4861 tccaccacac tgatacctgc gtcttcaatc ttttggatca atttgtccat ttcatctgca
     4921 ttcaaagtga atggtgtagc aagctgattg gataaatcat catatacaac ttgtcgtttg
     4981 ggtttgtttt ctttgatgaa agctgctact gctgcttcat acgttttttt atctgttcca
     5041 tttgccataa aagaaggccc cccttgagta gctatttggc ttgttttaac tgtttcgtta
     5101 aattgatgat ttcaatcgtc aattcaagct caagctgttt gtttccagtt cgtctggctt
     5161 cttgctgcat ttgttttttt aatgttattt catcagctaa gccagatttg ctgatcacat
     5221 ctagcaagtc ttggatttct cgtggcgaac tttcttctgc caccggtaaa tattcgattt
     5281 ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc taaaaactct gcaaggttga
     5341 attcataatg ttgtgagaca tatgcatcga ataaaaaata aacttctgca tatgcatcat
     5401 gaataaactg aaattctcgt tcttttaaaa tattacggac agaaacttct ttaaatgcac
     5461 gatataatag aatctgctct gccttttcta cttgcgtcaa aggtctcttt ttccgtgcag
     5521 tttcttgtct cgtaacctga gtgtgttgac tttcctgagg ttcttgacga ttttgctgat
     5581 ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc tctgccgatc tgaaactctg
     5641 ttgacagttg agatagatac atatcttgtt cgattggtga aacaactcgg ctaagttcaa
     5701 ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt atctaaattt ttcccttgtt
     5761 ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc atgagccact aattcttgga
     5821 aagatgcttc tccatactta cggagatatt catcaggatc aagcttttct ggaacgacta
     5881 cgacagaaag tgctaatcga ctgttttgcc gaagcaattc aatccctcga ttggttgctt
     5941 caaaacctgc gttgtctccg tcatagcaaa agaccaattc tttcgtcagc ttttccattt
     6001 gttggatctg ttgagctgtt agacttgtcc ccatagaagc taatccagtt ttgataccag
     6061 cctgccatgc agcaatcaca tccataaacc cttcgaacaa caaaatttct ccgtttttcc
     6121 ggatatcgct tcttgcttta tcaagattaa ataaaacttg tcttttattg aacagttctg
     6181 tttctggact attcaagtat ttgggctgat ctttttcctg ccctttttca ggtgcaagcc
     6241 agcgtcctga aaaaccgatc gtctggcctt gagaattacg aagtggaaac atcactcttt
     6301 gataaaaacg atcagacata cggccatcat catgcgtgac aaacaatcca gagcgttcta
     6361 acaattcatt tgaaattgat tcattcttga atacttgctg aagaaaagaa cgctcattag
     6421 gtgcaaaacc tatgttgaat tcttcaatta attctaaaga caacccacga ctttgcaaat
     6481 attctaaagc tggctgtcca gcttttgtat gcaatagcat gtgatgatag atttccgctg
     6541 ctttttcatg agcagcaatc agtttccccg tagcagattc aggattttgg acagctatag
     6601 tacgctgacg ccattgatct tctaacggta tctgttcgaa gtctgccact tttaccactg
     6661 cttctggaaa acttagacct tctagttctt gaagaaaagt aaacacgttt ccgcctttac
     6721 cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac agaaaaagac ggtgtacgtt
     6781 cttcatgaaa aggacacagc cccaagtaat ttttaccaga ctttttcaac tggacatatt
     6841 gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt ttcgataacc tcttgaggaa
     6901 tccgttgagc cattttttca ccccctgctc tattgatatc ttttgggttt aacagaagat
     6961 atttcaggag aataaccggt aaaaaatcgc actaactgaa atcagtgcga ttttaaagga
     7021 cacaattata catgtttgat tgtatcatat ttcattgctt tttcaagtat aacacatttc
     7081 tatctcatta agcatcaatt tgctactctt ttcagtaact gattttgaat atcagccttc
     7141 cagtacaata ctcgaagaac aatatttacg agcatgaata caaagaagta tcctacgctc
     7201 tctcccaacc ctaagtttac ttccaaaatt ccatggaaca gcattgcagc tacatatatc
     7261 cctacgatcg tcgttactgc taggaagatg cgcaatgggt tgaagggata acaagctttt
     7321 acaactgcca tgcaactgat cccaatcagc aagtaataca tcaaagtagt agtttcagca
     7381 tttccccatg cgaatgtttt tccaagtaga tacaccgcaa tgatattcac tactacaagc
     7441 aaggcatttg gtaaagcatt aataagtgct gtaggcaaat aacggtaagt gatttttctt
     7501 ttatcttgtt caaaagataa aaagaaggaa ggataccctt caatcgctaa atcaatcagc
     7561 gtgatttgga tagggataaa cggaaatcca attgctgtaa cggcacatat aatcgataaa
     7621 ataaatgaat aaatcgtttt gataaaaaag atgcctgaga ctttcgtcac attgttgacc
     7681 actcggcgtc cttcaaacag gacttctggc aatgtagtaa aatcagagtc caacaatacc
     7741 agattcgcaa tctgacgtgt tgctccatca ccttctgcca tagcaatgct gcaatctgct
     7801 tctcttaacg ccaaaacatc attcacaccg tcgcctgtca ttgcaaccgt tctgccgctt
     7861 tctttcaatt catttaccag caatttcttt tgctgcggac tcactcgtcc aaaaaccgta
     7921 tagcgatgag cagcttctcg cacttccatt tcttcagtaa tttgagaaag atcgacatac
     7981 gcatcataat tcggtaatcc tgcacgtcta gcgatattgg agacagtaaa aggattgtct
     8041 ccagaaatca ctttcagatc cacaccttcc tcatgaagat aagctagtgt ttcttgagcg
     8101 ttttttcgga ttggatcatc gatttcaaac agagcaaccg gctcaacaaa tgctggtttt
     8161 tcttcgttca attgctgatt tggagcgatc cccaacataa gtacgcgata gccattcgct
     8221 tgggctgttt ttagactgtc cggaagccga tcttcttcca ccaaacgttc aggcgctcct
     8281 acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc agaagaaaat
     8341 gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct catagcccgc
     8401 atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc tgggatggct
     8461 ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc ttcagtaatg
     8521 gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat tgaatacatg
     8581 tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa tgcaatactt
     8641 attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga tgcaataaca
     8701 gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat gatccccaaa
     8761 ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac aagttcagaa
     8821 cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga cagcataatt atctgcacct
     8881 actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga aagcaattca
     8941 gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc atttgcttct
     9001 gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc gatttttacc
     9061 aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc ccggatcact
     9121 agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc atgtatttct
     9181 tgataaattc caataacgat attgacgaca ataattgcta aatacgccat gttggaatac
     9241 gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa taacgtcaat
     9301 atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta gtcattttgc
     9361 tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat tttctttttc
     9421 tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg ttccaagatt
     9481 aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt acaaaaaaga
     9541 tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct atctttactt
     9601 atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta taaaaagaac
     9661 aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa tgatcataat
     9721 cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg cctttgagaa
     9781 aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa ttccgatcgg
     9841 cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat acaatcccat
     9901 atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac caatcaatcc
     9961 gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata attgagcgcc
    10021 gcataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca catcagaagt
    10081 atctcttcct tttttccctg cttctttcca taacgcagga acagttccag cgatacatcc
    10141 gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga ctaaaaaaga
    10201 aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca aaacattttc
    10261 tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca tgccaaagac
    10321 agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga taaacattcc
    10381 cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat tttccatttt
    10441 ttgccttttt tccactattt gcgcactcct tctttcattt aattttattc atgattgaac
    10501 atttcctgct attttactta aagacttttt cataggtctc tattattttt tcattcattt
    10561 tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg ccattgtaaa
    10621 gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct aaaagtgggc
    10681 tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg ccatgatttt
    10741 ttacataggc aatataatct ttcccaaaat tatatgcttg gacagctgtc cataaatcgc
    10801 atccttgttt ctttgcttct actatcattt ccgccaaata tttgacacct tgatcaatac
    10861 tgtcagctgg attatcaatg atctcagctt ctccatgcag actttccgag ctctgcatga
    10921 gatctgtccc ttttcctttt gattcggtca atattatcgc cttgatcaaa ttctcgtagt
    10981 tttccatatg ataatttttt gcagctgttg ttatttctat ttgatagctt tccactcgtt
    11041 ttaccgaacg ataagttctt cctcataaaa acaacaccgc accaataacc gtcagaatga
    11101 tcatgtaaaa aagaatacgg ataatagata atcgttttcg cttcatttca tcacctagtt
    11161 attttgacat ctttttttat cttactattt ctttatcttc ttggctattt cttccttgat
    11221 ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt ttttgacatc ttacgaaaat
    11281 gatcgttgtt tttcagtaaa aacaattgcc caatgactga tataatcgtt atactttaca
    11341 cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa aatgccagat gggagcgaaa
    11401 tttactatga aaagagcggt caaggcttcc ctctcttttt actgcatggc aatgacggca
    11461 gcggccgctt tttttcagaa caggtccctg tactcgaacg ctactatacc gtgtatctag
    11521 tagatagccg aggacatggt cgttcgacaa acgaagcatc tatgttgaat tttcagttaa
    11581 tggccgagga tctgaatacg atcatgttgc tggaaaaaat cgatcaggca gattttcttg
    11641 gatttagtga tggagcaaat ctagcactgg tatttgccag cagcttccct aaaaaagttc
    11701 atcgattgat tttaaattct ggaaacacgc ttgttaaagg cgttcgtttt tctgccagag
    11761 tcatcagtaa tattcattat gcatgggtat ggttactctc tttatttcgc ccaagtcttc
    11821 gcaaaaatct tttagttatc aaacttttgc tgcatgacat cgggcttaca gaaaatgatt
    11881 taaaaaaaat caactccccc actttgatca tcgtgggaaa aaaagatgta atcaagctaa
    11941 aacattcttt gtatatagct aaaactatac caaaagcttc ctttgtgctt gtgaaagaac
    12001 aaggacatga attagctaga aaagatcctg aacgcttcaa tcgagaagtt cttcaatttc
    12061 tttctgaaac ataactatta ggaatagaag gtgatttatt tgttaaaaaa taccatacaa
    12121 tggctgaaaa accacctagg actgttcaaa acaatctttt tgatttctgt cattgtcatt
    12181 attgtcggtg aattgatgtc catcggtaaa actctatcta tccagcaatt aggagaaacg
    12241 tttgccacta tacctgtatg gaaaagcgga ctaatgctat tgattggtct tatctctgtg
    12301 ttacctatgg ttggttatga tattattttg aatcggatgc ttgaacaaaa gcagaatcct
    12361 cgttacctgt ttgagacaag ctggctgatc aatacgatca acaatattgc cggtttcggc
    12421 gggtttgtta gtgtggggct tcgctctgaa ctatatggac aaaagaaaga gagcagaaac
    12481 gtgatccaag cattatcgaa agttttcttg tttttgatgg ctggactttc tatttacagt
    12541 ctgctttctt ttttacttgt agtcttcacg ccagttgctc cttttttgaa gcaatattgg
    12601 atatggttga tcggcggcgg tctatatttt cctgtcgtct ttttatttac tactttcaaa
    12661 aagaaaggat tccttggtgg tacctcactt aaaactcaag gacagctatt gctagtttca
    12721 ttgttggaat ggaccggtgt cctgtttagt ttcctttctg ttggttattt aatggaaatc
    12781 cggattgatc tgtggcaaac aattccatta ttcattgctg cttcagtgat aggaatcgtg
    12841 tctatgattc caggagaaat tgggagtttc gatgtaatga tgattatcgg gttgtccgct
    12901 attggtgtgc caagagaaat agtcgttgtg tggatcttgc tctatcgcct gttctactac
    12961 atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa atatcggttc cacttttgat
    13021 cagcgttatt ctggtatccc taaacaatta gcaacagaaa ttgcgcataa aatcgtggtt
    13081 gtcttattat acttttctgg aatcatgcta gtactttctg ccactatccc gcaagctttt
    13141 acagagtttc gctggcttca tagtttgaac ccattgaaat ttcattttat tatccaattt
    13201 cctagtatac tattgggatt tcttttaatc attatgggca gagggatcgc agcacgtgtg
    13261 aaacgagcat atttgcctac aatcttcttg attgttctag ctttacttta tgttttatta
    13321 agtgatttta gtttcacacc ggtggttttc ttgtctattc tgcttctgat cattcttgct
    13381 tctaaaaacg aattatttcg ggaacaattg atctattcat gggagtggcg tacgatcgat
    13441 ggaatcatta ttggtgcact gtccttgctt tacatcgtta ttggggtata caatctgccg
    13501 gatttccctc atcggcgtca ccattttatt tcctttttct tatttccttc tgaaaagatt
    13561 tggttttcgg gattgttggc catcatcgct gttagtttca tgatcgttct atttgttcat
    13621 ttccttcaag gagaaaagaa acagattggc gaagccttta atgaagaaaa ggccctgaag
    13681 atccttacaa cttatggagg aaactccgat agtcaattga tcttcttaaa agataagcgg
    13741 atgtttgcct atgaaaaaga cggtgaacca actgttcttc tccaattcgc ttgcttcaat
    13801 aataaatgta ttgtaatggg tgatccttct ggtaagaaag aagattttcc agaagcaatc
    13861 gaagctttta tcgaagaaac agatcgacta tgctatttac cagttttcta cgaaacaagc
    13921 gaagagattg ttatgatttt gcatgaattc ggctacgatt tcattaagat gggggaagaa
    13981 gcttatgtag acctgaatag ttttacaact tcagggaaaa aaatgaaggg cactcgtgct
    14041 gtcttgaatc ggatcgaacg agaaggattt acatttgatg tcttacaacc accattttct
    14101 actgagcaaa tgagtacttt caaaaatatc tctgataatt ggttgggttc acgaaaggaa
    14161 aaagggtttt ctttaggctt tttctctgaa gattatctac aaagagcgcc tattgcagtt
    14221 gtcaaaaata cccaagaaga aattgtcgct tttgcaacga tcatgcctac ttacacgaac
    14281 aataaagtag gaacgattga tctgatgcga tatgattcta aaaaagcacc ttctggcagt
    14341 atggatttct tgttcttgca tttgtttgcc tatatgaaag aagaacatat ccaatggttc
    14401 aatttaggga tggcccctct ttcaaatgtc ggaacttcca gaaagagttt tttacaggaa
    14461 aggattgcat acttggttta cgagtttggt tctcattttt attctttcca tggattaaga
    14521 gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt atactctata ttctagagat
    14581 agttggattg catatgttat gattgctttg ctgattattg ataatgcgcc agttgagcag
    14641 acacaaggta gaataagtgg tttgaaaaaa tggatacgaa gaaagtatta aaaactgcat
    14701 accgatagat gaacactctc atgatgaata aaagagactg aagtaaaacg aaagttcatg
    14761 cttctacaat ctcttcttat acaattgaag aacaaagagc ggtccaaaca gaaatcctgt
    14821 ttggaccgct cttttatttt aaacattttt cattaccaaa atattcgagt cgcgtttctt
    14881 atgcttcgat ctctgtaacg atacctgaac ctactgtccg tccgccttca cgaattgaga
    14941 atgtcgttcc gttttcaaca gcgattggat ggatcaagtc aacatcaatc gtcacgttgt
    15001 caccaggcat gaccatttct gtatcttctg gcaatgtgat tgttcctgta acatcagttg
    15061 tgcggaagta gaactgtgga cgatagttgt tgaagaatgg agtatgacgt ccaccttctt
    15121 cttttgtcaa cacgtacact tctgctttga attttgtatg tggagtaatg gaacctggtt
    15181 tagcaagtac ttgtccacgt tcgatatcgt cacgttggat cccccgtaac aacacgccta
    15241 cgttatcccc agcttccccg taatctaacg ttttacggaa catttctaca cctgttacaa
    15301 ctgctttttg tgtttcaggt ttgatcccta cgatctctac ctcatcaccg acacgaacag
    15361 caccacgatc aatacgacct gaggcaacag ttcctcgacc tgtaattgag aagacgtctt
    15421 ccactggcaa tagtaatggt ttatctgtat cacgttctgg tgttgggata tattcatcta
    15481 ctgtatccat caattccata atagcagctt cagcatctgg atcgccttgc aatgctttta
    15541 atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa accatattcg ctcaataact
    15601 cgcgaacttc catttctacc aaatcgatca attcttcatc atcgactaaa tcaactttgt
    15661 tcaagaaaac aatcaggtat tttacaccaa cttggcgtga caataaaatg tgttcacgtg
    15721 tttgaggcat cggaccgtca gttgcagaaa caactaagat tgcaccatcc atttgtgctg
    15781 cccctgtaat catgtttttc acatagtccg cgtgtcctgg tgcatcaata tgcgcgtagt
    15841 ggcgtttttc tgtttcatat tctacgtgtg cggtattgat cgtaatccca cgttcacgtt
    15901 cttctggagc tgcatcaata ctagcatagt cttgaggatt tgctaggcct tttttgccta
    15961 atactgtagt gatcgcagca gttaaagtag ttttcccatg gtcaacgtgt ccaatcgtac
    16021 caatgttaac gtgtggttta cttctgtcat aatgttcttt tgccataatt tacaaacctc
    16081 ctaaataata tagatcactg aaaaatttca gctctcacct ttttattata gtcttttttt
    16141 gactaaatca aaaagaatac gtccgactta ctttttgtaa gattcgaact aagcttatta
    16201 tacgtcaata ttggtcaaag tcaactaaga gcactcgtct gtcttatacg cgaaaaaagt
    16261 accttttata aataaaaaaa tgtgtttttt gagaaaaact tcaagatagt ccttaacttt
    16321 cttttgaagc aataagaaca aaatgaggaa aagtttaaaa ttcagctgtt gccaacaact
    16381 ttcaatagac gaaatcttct atttcgttaa taataaggaa agaggtgaag tacatgaaaa
    16441 aattaattgg cgtacttgtc gttttaggca tatttctcgg cggaagtttt gcagcttatc
    16501 attattttta cggcggcgaa gcttattata caaaaatcac gacgaatggc gaaatgtcag
    16561 ctacccaagc agataacgga gagaaattca cgacttatac ctacaagcaa gcggcttata
    16621 ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga aagagaacat ccattaaaga
    16681 tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg tgtattaagc tgggaagaaa
    16741 taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat cgataattag taaatggtct
    16801 taaaatttta ttagataaaa
//
LOCUS       DAHUVV010000055        16377 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.056, whole genome shotgun sequence.
ACCESSION   DAHUVV010000055 DAHUVV010000000
VERSION     DAHUVV010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.056"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(107..1687)
                     /locus_tag="NK767_001785"
     CDS             complement(107..1687)
                     /locus_tag="NK767_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCH6694780.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(1680..2423)
                     /locus_tag="NK767_001786"
     CDS             complement(1680..2423)
                     /locus_tag="NK767_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6694781.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(2599..3375)
                     /locus_tag="NK767_001787"
     CDS             complement(2599..3375)
                     /locus_tag="NK767_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6694782.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(3623..4459)
                     /locus_tag="NK767_001788"
     CDS             complement(3623..4459)
                     /locus_tag="NK767_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HCH6694783.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(4470..5399)
                     /locus_tag="NK767_001789"
     CDS             complement(4470..5399)
                     /locus_tag="NK767_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCH6694784.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            5591..5944
                     /locus_tag="NK767_001790"
     CDS             5591..5944
                     /locus_tag="NK767_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCH6694785.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(6018..6674)
                     /locus_tag="NK767_001791"
     CDS             complement(6018..6674)
                     /locus_tag="NK767_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCH6694786.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            6900..8834
                     /locus_tag="NK767_001792"
     CDS             6900..8834
                     /locus_tag="NK767_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCH6694787.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(8920..10143)
                     /locus_tag="NK767_001793"
     CDS             complement(8920..10143)
                     /locus_tag="NK767_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCH6694788.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10137..12035)
                     /locus_tag="NK767_001794"
     CDS             complement(10137..12035)
                     /locus_tag="NK767_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HCH6694789.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(12029..13147)
                     /locus_tag="NK767_001795"
     CDS             complement(12029..13147)
                     /locus_tag="NK767_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCH6694790.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(13230..14063)
                     /locus_tag="NK767_001796"
     CDS             complement(13230..14063)
                     /locus_tag="NK767_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6694791.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(14056..14973)
                     /locus_tag="NK767_001797"
     CDS             complement(14056..14973)
                     /locus_tag="NK767_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6694792.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(15200..16324)
                     /locus_tag="NK767_001798"
     CDS             complement(15200..16324)
                     /locus_tag="NK767_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCH6694793.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         5182 a         3255 c         2668 g         5272 t
ORIGIN      
        1 tcacattttt acataaaatt gacttaatac tgagaaactg acttttgaac accctctatc
       61 tgtaaaaaag gggctgcgac atgatttttg tcacggcccc tcttctttat tcaaattttt
      121 tccaaaacgt tcgttgataa taaacgatcc atagagcgga aagtattaga actaaaccga
      181 tcacaggaat attcactaac caaaatggat aaaacaaagc caacattccg tataagacga
      241 taagaataat actgatgatc aatgaaccca tcatagttgc gactaaaccc aaacttcctg
      301 aacctctgtt aaataattgg ttaatattgg tccagttcag cagcagcaac cgataatctc
      361 tagcgaaata acgtagtgac aataagtagc agccgaataa agaaccaaat agcaagctag
      421 ggataaaaaa caaaggcagc tgaaaagaca gtccgcttaa taatgcgatc ccaccagtta
      481 aaaaactctg caatatccat ccgaatctga acttttcttt taaatattgg ttcatagaga
      541 ttggcaaaga acggataaac agaaaatttt cttgatccaa agaaataaga ttgctgataa
      601 aagaagtctg attaactgtt aagaaagcca atgcgatccc taccaggaaa acaacaccga
      661 ttaacttatt atctaaatca gttaatttta tttcgcctcc tagggcaaaa gcaagaataa
      721 aaatgattgg agttaacaag gaagaagaaa gaacttgcat aatcaaatta ggttctttta
      781 ctaattgagc attgtaacta aataaaagct gatgcacatt ttggttcttt ttatgggttc
      841 tttttatttt gaactcactg tttgaatcag tcagttgctc tgtaagcttt ggtactatca
      901 gctgttttac agcaaggaaa aatcccagag ccgtgatagc tagtattccc caacccaata
      961 aaccttgttg agaaagtggc tgactggcag catcatagaa tggcaataat atcgaaatcg
     1021 gatgccgatc tgttaactca ttttcaatag tccccgtttg atgattcatc cacatgattc
     1081 cagcaaatgc gatcagcatc gagctgccaa gcaagatact agaaactgtt tttctatgtt
     1141 ttttaaacac tgctgttcga gccaaaccaa aaacaatcaa actagccaga caaaaaagaa
     1201 taatcaaaaa tagagaaaac aaaaggattg agataatgat agtaagcaaa atccctagat
     1261 cagctcgcca tccggttaaa ataaacatga ctagaagcgg aaaaacaaaa ggaatgatgg
     1321 tcaaagcaac taccaaaatc tttgctgaaa aaacctgttc ttgtccaaaa ggcagaggca
     1381 aaaatacttg caggtcattt ccttcaaaaa agatattata aatgactgat atactttgag
     1441 ttaaacctaa cataccaaaa agtgccacgt aataagtaaa gaaacctggc aatttaacaa
     1501 agttcatagc aaacatagca aatccgtaaa tcagtaaaaa aatcagccct gaaaaaagat
     1561 tttgagacaa aagatagata gttaatcgct ttcccttttt tcctttctct cttgctttat
     1621 tcgtgagctg tggattaaca tataaaagat taactcgaat taattctaat aattgtcgct
     1681 tatacatacg gtccatctcc tgtcgcttgc cttccagcca ttttcaaata aatcttttct
     1741 agcgtttcat caggtgagcc attcaataat tcactaactg ccccctgata aatcaattca
     1801 cctttcttca aaatagccag tttatcgcat aactgttgtg cggtatccaa tacatgtgta
     1861 gaaaaaatga ctgtttttcc ttttgcggca tgagccttca tcatttcttt caaatcaaat
     1921 gctgcttgag gatcaagtcc ttgcaaaggt tcatccaata cccaaatatc tggatctggc
     1981 agcaacgtgc cgatcaaaat cgttttttga cgcattccat gtgaaaaact tgcgattgtt
     2041 tcattttgat gactatacat atcaaaaagc gctgtcaatt ctgctaaacg cttttctttt
     2101 ttttgtgtgt ctaattcata ggcagctgaa atcaagtccc aatattctcc agccgtcaat
     2161 tgtaaaaaaa tatctgggga atcaggcaca taaccgattt tttgtttgat actcaatcga
     2221 ttttccgtca atttcattcc atctactaaa atcgttcccg atgatggttc aataatacta
     2281 actaaacttt taatcgtagt tgattttccg gcaccattat gtcctaaaaa tccaaaaatt
     2341 tccccttgtc ttatcgagag agacaagtct tttaatgctt gttttttccc gtaactttta
     2401 cttacattga tgaattcaat catttctgta ctccttttgt ttgttattac tttttattgt
     2461 acccttcgtt attggtggag taaatgtcaa aaagagtgca taacaaaaaa ggatagaaac
     2521 gactaggctg ctacttgggt tgttctcttc tacaggaaaa cactcttcta acaggtaaga
     2581 tatgagtctt tgttttcttt aaaatgattc attttctatc ttttcttgca taaaattctt
     2641 tagtttatca atacttctct ttcccatctc tagttgttct ccatttgcaa atgttactat
     2701 ttcgtttact ctgctaatag atgtaatgtt ttcagtatta atgatataag cttttaaatc
     2761 tttacataaa tactcatatt cgagtgattt tttatatttt ttaattgtgc cgtttacgag
     2821 aatctcttca tctttcaata ctaggcctat tttattttta taacctggca tgctcattag
     2881 ataaaggatt tcatcgtaat ggatatagtg atcgctatta ccataacgga cagttgtcca
     2941 cctttttttc gtagcatgtt gaacaggata gttttctttt tctttcaata agtaactttg
     3001 gatgcttttt ttattttttt cataagtgaa ttcttttatc aaataaccac taggcgtaat
     3061 ctttcgatta ataacagtta ttgctttatt cggatgagac gttagataaa caattttaca
     3121 cttttcattc ttttctctaa gcatttcgcc tagttcgata ccgctataat aggcgttcaa
     3181 atcgatgtct aggaaaaaaa tatctgagga atagatttca ttatttttaa tgaattcaac
     3241 aaattgtata acgttcaatc cctgaacgat ctcaaatttc caaggactta ataaaattct
     3301 ggattcttcc aaaatttcta ctagcatttt ttcaaaaacc aaactatcat ccaaaataaa
     3361 tatacgcgtt accataaaaa gtacctccac tcgatatttt actatcaaaa tcatttcata
     3421 caacttttca acatacatgt taggaataaa ttgtaattct cttcacaatc ttttccaaca
     3481 cctttgaaac cgatatcaaa tactttcagg atgctgcttc taaaataaat atcaaacaaa
     3541 tgaatacatc atgcaaaaaa agccagacaa ccgacgtcct tgcgtgtcag ctgtctgagc
     3601 tatgtttatt tattttgttc ttttaaaaag aacgatacac atactgattt ttcggagcag
     3661 agactttctc agcgtttgta acaactacag atgggatttc acgcttgaat ggttgataat
     3721 agtctgattg gatggttcct tctattttat accattcatc atttttaacc gttactcctt
     3781 ccggcatatg cgtcaacaaa ccaaagactc ctgaatctgc cacacaatgg ataatgccga
     3841 atcggaacaa gaagatatct gtattgctat cgttcggtgt ttggtaaaca aatccttcat
     3901 agctgatttt tttgccaatg aattcgctag ggtaattata aattagctcc ataacttcta
     3961 aatagttttc atctgtgatc ttgatagttt gcttatcccc atacttttct ttcaactcat
     4021 tcatttggtc taagtagtct gatttgttga aataaatact ggtatccggc tttaagtatt
     4081 gtgtctgcat ctccggatcc cctactgatt ctttacttac tgggaaatta aatcctttgg
     4141 cttctacgat cgtagtgtcg agactaactg ttggaaacaa cgtccccacg attactggca
     4201 aagctagcag tacataagcg attccacgtt gataaggttt tgacaaccca tggtcatgat
     4261 cgtgctcgtg tgccttttct tctgcttttc cgtttttgac ccacaaatac agctgaacta
     4321 aagccaaaag agcagataaa atcattgaaa gaatagctaa gtagcgataa tggacattga
     4381 tatattgatc gagtttgcca gaaacttgca agtacatcat caattcgaaa tacccagata
     4441 aaatcaaaaa ccgcagcata ttttccctcc taaataaata atgtgaatcc aataacgcta
     4501 agggcaatca gtccgatcag cgataaaata aagcgtcctg taaaatatcg tttcatcatc
     4561 agtagatttt tgatatcgac cattgggcca aagaccaaaa aggcagctac aggtgctgtg
     4621 ccgaacagac ttaacaaaga agatcctata aacgcatctg cttctgagca aagagacatc
     4681 gtaaaggcca ataacagcat gatgataatg gctaaaatct ttgttgagcc gagcgttagc
     4741 ataattctag ttggtaaata agtctgcatc gcagctgcga ttaatccgcc aatgatcaaa
     4801 taacgacctg tatcaaaaaa ttcgtcgatt gaatgtgtca acacatgccc agtttgatgg
     4861 atcagctgat tttttggttc attcttctca ctttgttctt cttcatggac atgatgatgg
     4921 tgattttctg ctactttaga cgtgattttt tctttcaata cagcgtcttt ttgaaaatag
     4981 gcaagccaga caccaatcac aagggcaaca aaaaagctgc ctatcacccg ccagcttgcc
     5041 atctgccaag aattgccaaa tgcgataaaa gtggaaaaaa tcacgattgg attgatgatc
     5101 ggagcagtca gcataaaagc aaacgctgtg tgaactggta cgcctttttt aacaaattga
     5161 tgaacgatcg gtacgatgcc gcattcacat gaaggaaaga aaaatcctaa gatcgaacca
     5221 gtaaaaatag agaagatacg attttcagga agccatctct tcacacgttc tggggtcaaa
     5281 aatacttgta aggcgcctga gataagacag cctaataata cgaaaggtaa tgcttcaata
     5341 acgatcgata agaaaatcgt actcatctgc aaaaccgaat ttggaataaa agaaaacatg
     5401 agtttcctcc tttttatttc tagttcattc gctttgcaaa tatacgcttt tattcaaaaa
     5461 aaacaagttg ctctgtaaat ttttacacaa ctcttatttt ttaccaaagc acttcaattg
     5521 actttttctg ttattcccat taaattaagg tctcatacat atcacttcta tcataaaagg
     5581 gagaataaaa atgaaacaaa ccactaactt gactgaagtt caagacatac tgcaacagtc
     5641 atccgttgcc gtcatttatc tttcgatgcc taattgcagt gtttgtcacg ctgttcgtcc
     5701 acgactagag gaacttctca cacattatga tattccggca cttcatttgg atgcatttga
     5761 aacaccagaa gtagctagta aatttgaagt gctgactgcg ccagttgtcc tgatttttca
     5821 tcaaggaaaa gaagtctttc ggcaagcacg gttcatcgac tttaaaaaaa tccgttatct
     5881 actagatgaa ctgacagcag aagatgattc cctttcttat gaagaaatct tccgaacaaa
     5941 ataatcgttg tccaaacaga aaaaagcgat tgaacaagtg aaagttttgc cttcatttgt
     6001 tcaatcgctt tttattttta ttctgctgta ttttcggaat gcgcatttgc gtcagaatgc
     6061 aagaaataga tcaaggtttg caattcattc gtcaaatcaa cattttggat caatacatct
     6121 gacggagcat tcaatcgagc agcggtaaag ttcaagattc ctttgattcc tgcttccgtt
     6181 aactgattga ctacttcttg tgctctttca gcgggtatcg ttaaaattgc tacttcgatt
     6241 tgttggatct tgatttgttc tgacatatct tccattggat aaacggggat accgtctacg
     6301 atgcgcccaa cgatttcctc attcacatca aaagcacaac tgatacgtat gctattactt
     6361 tgatggaatt tgtattttag taaagcactt cccaagttcc ctacaccgat cagcgcaaca
     6421 tttgtcaatt gatcgtcgtt taatgtcttt gcaaaaaagt gcataagatc ttctacatca
     6481 taaccatacc cacgttttcc taattcgcca aaataagaaa aatctcgacg gatcgttgca
     6541 ctatctactt gaacggcttc acttaattca gttgaagaca cttttttctt tccagcatta
     6601 tttaaaatcc ttaaatagcg ataatacaaa ggcagtcttt tagctgtcgc ctttggaata
     6661 atatgatctt tcacgaaatg acctccacgt attgtctaat tacatctttg atttctccat
     6721 catagcagta tttataggaa aaaatcaatt ccattgctca gatattgtga aattttattc
     6781 tttattcatt tatttatcat ttctgcaaat ctcgcagtat aatggaattt atggtagact
     6841 actaattgaa aaatagtttt ttatttgagg agaattcaca aagtagaaaa gaggattcca
     6901 tgatattatt acaagccaat cagattgctc gccattttgg tgcagaagtc ttatttgaaa
     6961 acatacattt agaaatcagt acaggcgctc gtgtcgcttt agttggcaga aatggcgctg
     7021 gaaaatcgac tttattaaag atcattgctg gaattgaagc acctgatgaa ggaaagatcg
     7081 ctaaaaataa aacagcgact ctcggatacc tcgctcagga cacaggactt gattccaatg
     7141 aaacagtttg gaacgagatg ctgaaagctt ttgaacaagt tagacaaatg gaagagcgga
     7201 tgcgcgagct agagattttc attagcgaag gtacgccaga cacacctgct tatagttctt
     7261 atttaaaaga atacgaccgg ctgcaacacg atttttcaga tcgaaacggc tatggttatg
     7321 aaaatgaaat tcgttccgta ttgcatgggt ttaaattcga ttcttcattt tatgatcaga
     7381 aaatcgatac tttatcaggc ggacagcgca cacgtttagc tttagctcgc atgcttttgc
     7441 aacaccctga cattttgatt ctagacgaac caacgaacca tttagatatc gagacacttt
     7501 cctggcttga gaattatctg caaagctata ctggtgcatt attgattgtt tcccatgacc
     7561 gttatttttt agataaagta gtcaatgaag tctatgaatt aagcagaaga aaaatgcgcc
     7621 attacaaagg taactattcg aaatatctag atttgaaagc agaacagtta gcaagtgaat
     7681 ggaaggctta tgaaaagcag caaacagaaa tccataaatt agaagacttc gttgctcgga
     7741 accttgttcg cgcctctacc acaaaacgag cgcaaagccg cagaaagaca ttagaaaaaa
     7801 tggaccggct ggatcgtcct caaggggatg aaaaacaagc aaactttcta ttcagtatag
     7861 aaaagccttc tggaaatgta gtcttgcaaa tcgaagatgc cgctatcgga tatgataatg
     7921 tcaccttatc tgaaccaatc aatctagata tccgtcgtca agaagcgatt gctctagtag
     7981 gtccgaatgg tatcgggaaa tcgacgttgc tgaaatcaat cattcaccag atccctttta
     8041 tccgtggaaa agaaacgcta gggacaaatg tcaccatcgg ctattacgat caaggtcaag
     8101 ctgatctgca tacaaacaag acgatcctag cagaattgtg ggatgagcat cctaccactc
     8161 cagaaaaaga gatccgaagt gctttaggtg ctttcttatt ttctggcgaa gatgtagaaa
     8221 aaccaatccc tctgctaagt ggcggtgaaa aagcacgggt tgctttagcc aagttagcga
     8281 tgaacaaaga aaattttttg attttggatg aaccaacaaa ccatttagat attgacaaca
     8341 aagaagtatt agaaaatgca ttgatcgatt atgacggcac actgcttttt gtttcccatg
     8401 accgatattt catcaatcgg atcgcgacaa aagtcgtcga actctctgaa aatggcagca
     8461 aagtctatct aggagactat gattattatt tagagaaaaa gcaggaagaa gaagaaatcg
     8521 ccgaattgtt ggcacaagat gagccagaat ctgctcctgc tcccacgaac aaatctgatt
     8581 attaccaaag caaagaacag caaaagctta tccgtagcct tcaacgaaaa attactcagg
     8641 ttgaagaaga aatggcgcgg atcgatgaat ggatcgatca gttggaacaa gaaatggtcg
     8701 cgccagaaaa cctggaagat cacgtaaaac tcaatgagct gaaccaagat ttagaagctg
     8761 ctagacaaga tcaagaaaca aaattgacgg aatgggaaga actaagcttg gaattagaag
     8821 aatatgaaaa ttaacgaaaa aaccaaaaca gaaaccattc atgctgtttt ggtttttctt
     8881 tttttatttt atcaaacaaa aattccatat ttaccttttt caccaataag aagaccaatt
     8941 actagtcatt ctcactaaag cttctaaata atagtaatca ccccaaatac aaggtgtatt
     9001 taccccatta tttttgggct tattataaac agactgtgtt aaaaagccat cttcttcttc
     9061 cgttgtagca tatctttctt tcaattgtcg cataattttc caaacagcag actggtataa
     9121 gttcttttct gttttgtcta atagacctga ttctattaat tctaataatc cacaaaccat
     9181 gatcaccata ctagatgtat ctttttcttc tctgctagaa tcatcaaaga tcaaatccca
     9241 ataacaaatg gtatcttctg gcaaccgatt cataaaataa ttggctaata ttttgcttaa
     9301 atcaaaatat ttttgttctt tcaaataacg ataacttaat gcaaaaccgt aaatccccca
     9361 tgcttggcct ctagcccaac aggaattatc actataacct tgatgcgtac ttccatatct
     9421 tggttttcca tctttgacat ccatataaaa tgtatgataa gttgaaccat cttctcttac
     9481 taaataatct gctgcatttt gaatatgagc ctgagcagct tttttatatt tttgatttcc
     9541 agtttcttca ctcgcccaat acaataatgg aagattcatg caacaatcca tgatcattct
     9601 accttgttgt gaaggatcac taggatctcc ccaagcttgt ataactcctg ccttctccca
     9661 aaaacgctga agtaatgttt ctgcagcatc aattgaagct tccttcattt ttcgatttcc
     9721 cgttatttta taatctgcta cggttgataa agaatacaaa aaaccaagat cgtgatgatt
     9781 agtgataatt tgacagtcaa tcctgtggat aaaatctata tctgcttttt cagctgtttg
     9841 ttgaaataaa ggatcacccg tcttctcaaa taacaaccaa ttaattcctg tccaaaatcc
     9901 ttctgtccaa ccaatattgt ctcttaatgg ataacgtcct tttataggac actcgcttgg
     9961 gaattgatac tgactgttac tgatacgttt gattaacctc tccgtgtgtc tgcgacaacg
    10021 ctcgatctca ttattccatt ctttttccat taattcttta taacattcat ttatccattt
    10081 ttctggatgt attatctctt caaacatcac tttatcaagc atttccccac cagcctttag
    10141 cataatttta gtcttttttt attgttttta ttttggatag ttagcccggt tgtcccatca
    10201 aaatcaattt tgggacgatt gttccatact tctaaactat aatcccgatc ttgatgagca
    10261 accactcctg ttccgagcca atgggttcct tttccaaatg taccctctaa tccaggaata
    10321 aaagtgtttt cccatgtcat caagtttgta ttgggcaaac ccataatagc tgtggatttt
    10381 cgtttaatag tatcgtcact taaacagata atacctgaaa agccagcttc actcacccta
    10441 tagttttcct tgtcttcttc tatgattatt ccttctttct caggagaaca ttcaatagca
    10501 aaccctgtct catatagaac atattctgta tctaattcta ctttgtgtac gcgaatctgc
    10561 cagccttcaa aaggaataag ccaagttctg atttttgcct gtaaagtagg ttcccatgta
    10621 gaggcaagta cctctttctt acatacaatg tctcgtacat tatttcgaac gatgaactgt
    10681 ccataggttt tgatgctctc attcttaaaa ctggtcgtga tatatgtgtc cgctcgctga
    10741 atccctagca ttgaatccat tgccatttct gctttcaatt gattcgctcg cggtatcgaa
    10801 aaaccaaatt gactagaata agcaaatttt aaatattttt cattggtcaa tgtatggaaa
    10861 tttggcccta attgtcccgc attcaaaaaa aatgtatgtc cattatcatg ttgtatgagc
    10921 aaattggctt ctgaaagaag tttagttcct tcacgaatag gcataggcat ttctttagcc
    10981 gtccaatact catggtcttc aggtagcgcc aacaataaaa aaatcttatt cagccataat
    11041 ggagataacg tactattata gggttcagtc aatgtcaatt gagggtaggt ataaccaata
    11101 ttcaggatac ctttttcatc aaatatttct ttttgcatcc accatcttat attccgattg
    11161 actaacccct tgatttcacc taatttaaaa ggcaaaagat cggcccacaa catagctgac
    11221 caaaagcttg aaactgcaaa acgataaatc atgcttcgac caaaaggcac atttgcacca
    11281 tcttccgcaa aaaaatgaat atagtctcta gaaaaatcaa tagctcggtt cttatatatc
    11341 tggctaatgg gcttttctgg ttctagtacg ctataaatca aaccataata atgaaatgca
    11401 aaaggattat aataatcttg gcgacctctt gaactgtctc gataccagcc attttgaaga
    11461 taacaatttt ctatattatc aaatgcttct ttcagtgctg attctttaac tggataacca
    11521 aatttttgga taattgttcc gacaattatc ttaaaaaatt gccaattacc atctgcacat
    11581 tcatattcaa aaatttgcga gaaccaatga ataatctggc ttttttctcc tgtatctaat
    11641 tttttccata ataattgttc atgatgatat aacgctaata acattgcggg catttctacc
    11701 atacgctggt ctctttcatg tatttctccc caataatcgg gatgggaagg atctgttcca
    11761 gcttttattc ctgatagaat taatttcaac catcgttcat ctaattggtc aattgcgggc
    11821 cccgctcccc ataaaacccg agagaatcct tccattcctg caattttatt tccatatcct
    11881 accccttgat gtatataatc aatttgtgcg ccacttgctc gataaaacgg ctggagtggt
    11941 tgatttatct ttttaaataa gtcaaagtaa tcctgtctgg tccttaatag tggtactgga
    12001 tgtttcattg tattttctcc tctttttact accaaatagc taattccgac tgttctaatc
    12061 gaagtaagcc ttcaataaaa aaataatcag aatagatcag tggcacatta taagtcagtt
    12121 ctctatggta tgcttctgct ccgcctccaa tgatcccatc ttcatccata ttccaatttg
    12181 catagtgctt ttctgtcttt tggagtatat tgatagcagc ttcatgatac atttttgatc
    12241 gtacagagga aacatatcct gctaattcca gaagaccgca tgcagcgcat actccagcac
    12301 tggtatcaat attcgaatct tttacagcaa aatcaatgag aggaatataa ccagtttttt
    12361 cgattttctg aataaaattt actgctatct tttctgctat agctagatag tacggatttt
    12421 ttgtgtaacg ataacttaac gcgaatccat ataaagccca tgcatttcct ctgctccaac
    12481 aagaattctc attgtatcct tgcccgccaa gtaaccgaat atatttccct gattctgcat
    12541 caaattctgc gatatgcccg gttgatccat ctttcctcac tagatactta gctgttgtat
    12601 ctgcatgctg ccgagcaatt ttagagaacc tcggatcacc agtttccctc gaagcccaat
    12661 acagtaatgg taaattcatc atactatcaa taatcatcca accaggttta tggtcattcc
    12721 atgctactaa atagttccct gaaatattat agcgtccagc aagtaccgta gctgcatgta
    12781 accctagttg tcgactatgt ttgttttgag actgttgata atgtgcaata gccgatggta
    12841 agaataaaaa acctacatca tgatgaacgt ctgaaaaatg gtacagggca gattctaata
    12901 attgttcttg ttgtattgct gcctctttga acttttctcg ttttgtcttg tgaaagagct
    12961 gccataaaat accactccaa aaaccattgg tccaccagtc gattcctctg tcctttatta
    13021 cgtctgtata ttttccgttg taagaaatat atggaatttt attccccatt ctgtctaatt
    13081 ccgcatctat cttttcagaa gctacattca aaatttttct tgaccattcc ttcgtctgtt
    13141 cattcatgga tatcccgcca ttcttttaat ttttatgaat gtggagttgc tcctagaacg
    13201 aactagatgg ttaacattcg ttattttttt tacttatttg cagctccaat gtcattctcc
    13261 ttaaatttag actgcgccca cacataaatc aagaccattg ggatcactga agttgctaaa
    13321 attagcataa ttgcgccata attaattggc tcacctgtac tagaaaccgt catatttttg
    13381 ataattaagg gaaccgtata tttatcttta tctgaaagca tgactaatgg aaatagatat
    13441 tgattccaac ttcccataaa gataagtaaa ccactagtaa taatcgtagg tgataaaatc
    13501 ggtacagcga ttttcgtaaa aatcacccac tcattggcac catcgattct ggcagcctcc
    13561 atcagtgctg taggaaattg actgctatat tgtcgcatca taaacactga agttgtactg
    13621 gcaatcgaag ggatgatcac acctgcataa gtattcgata atcctaaacg actcataatg
    13681 taaaataaag gaatcaatgt ggtaaaaaaa ggcaccatcc tagatagcat aactgtattg
    13741 aacaacagtt tattccccat aaaacgatac tttgctaatg cataacccgc cattgtagaa
    13801 ctggctgtag ctgaaactgt actgattaca gcaataacaa tcgaattcca aataatcaag
    13861 ggataattat atctcgccat gatggcttgc cagtttttta atagcatatc cccaaacttg
    13921 aaattgattc cgccacttaa aatttcttga ttactttgcg ttgaagctac aatcatatat
    13981 aaatatggca tcaaaaatat taaggcacca aacacaataa tgacgtacgt aaaaattctg
    14041 ctttgtatct tttgtttagt catttttatc acctccgaat ttaaattgaa tagccgataa
    14101 tataacagat acaaacacca tcgtccaggc aatagcagag gcttttccaa aatcaaccat
    14161 ttcaaaactt gttttatata aattgactcc tacgaccaaa gctgaatttt ctggtccatt
    14221 ggaagtccca aagaatatat ttgggatatt gaaattaccc aagccattga tagtggctaa
    14281 aactgtggcc attaaaatga ttggctttag catggggatc gttaataaaa agaattgccg
    14341 aaatttcgat gctccatcta tttcagcact ttcgtatatc tctttagaaa tcccttgaag
    14401 accagctaaa aataaaatag tgttatatcc tacactaagc caaatcatcg tgatgatcat
    14461 tgatattcgt atccaaaaag gatttgttaa ccactgtaca ccgttaattc ctagcgcatt
    14521 caaaaatcca ttgaataacc cagtcggatt gaaaaaagtc atgaatacgg aagctacagc
    14581 caccgttgat gtaatggttg gaatataata gacagttcta aaaaatcctt ttccttttaa
    14641 ctgtgctgaa ttcaataaaa cagcaaagta aatagctaat agcatcgtta ttggaattga
    14701 tcctgccatg ataatcaaaa cattcgtgta agatttaata aacaatggat ctcggaagat
    14761 agctagataa ttgtcaaatc caacaaattc aaatgagtag ccaattttca taaaactaaa
    14821 agcaaacgag ataatgatgg gaatcaaaat cgtcacaata aagaaaataa caaaaggtgt
    14881 gaccaatcca taagcacttt ttttatgaga cgtatctgtt gcttttttct tgctgttctt
    14941 tattgtcttg cttggtaacg aagattgcat catcgttaag tcctcctaat attttttaat
    15001 caccagttaa ttttacgccg tacttatgag caaaattttc tgcttctttt agatatgaat
    15061 tttcaaaatc acctgtttgc caatagttat atgcagcggt agtcaaataa gacgtagcat
    15121 ctgaataagt ggctggtaaa acagtatttg atgtattttc attgatttta ttcaatagtt
    15181 catagtattt ttgtcctcct tagtagtcaa actttttctc agctgcttct gtttgatagg
    15241 ccaatgagtt agatgtagcc acgcccagct ccagtgcgtt ggctaagcat tcttcatctg
    15301 tcagcataaa tgtaagaaat tgttgtgcca cttgtggatt tttggcatct ttagggacat
    15361 aaaaacttga tcctcctgat actgcacctt ttcctggatt ttcttttgaa aaaggaatca
    15421 gtggagccat gcgccatttg tcagatgcat caggaaaatt caaagacata ttcgtagcat
    15481 accagccgcc agcaacaaac attgcagatt cttgaaatgc tttctcagca tcttgtgcac
    15541 cgtagaaatg tacaatatct gagtcgatca tttctttgat taactttgca gcatgtttcg
    15601 cttctttagt attcagacgg atatttcctt cttcatcaag caaagattga ttctgctggg
    15661 ctagcatgtt gataaacaat tcttggtccc cagattctgg caaggcaatt ccaaaactac
    15721 cagttttttc ttttaatgct tttgccacct ggatatattc ttcccaagaa ctgatttctt
    15781 caaaatctac acctatttca gcaaattttt ttggttgata ataacaaagt gctggggtaa
    15841 aatcactagc ccaaggaata atcgtttcat tactcgtttg ttgacgcaaa atattcaatt
    15901 tttgatcata aaactgatcg ccatatttgt cataaaatcc atattcatca gcactataaa
    15961 aagcatcttg aaatttttcc agcacacctg ctgctgaagt gtctgccatg gagataatat
    16021 ccggcatatc ttcgtgtgtt accaattttg gggtgatcat ttgtactgtt gctgttcccg
    16081 aagctacatt cacaattttt aagtcaatat ttgctccagt tttgcggtta tagatttttg
    16141 cagcattttc aacatagttt gtcagtttgc cgtttgcgac atatacttct aatgtagtgg
    16201 ctttttttat cattggatct gcgatgtttg ctgttacttc ttcgttagtt tttccacatg
    16261 cacttaagct gaataatagg gtaatgttta gcaaagctat caccaatttg tatctttttt
    16321 tcatgtctat cccttctttc ttttcaagtt cttatataaa acgctttcac ttatgta
//
LOCUS       DAHUVV010000056        16177 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.057, whole genome shotgun sequence.
ACCESSION   DAHUVV010000056 DAHUVV010000000
VERSION     DAHUVV010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.057"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(22..957)
                     /gene="murB"
                     /locus_tag="NK767_001799"
     CDS             complement(22..957)
                     /gene="murB"
                     /locus_tag="NK767_001799"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCH6694794.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1030..1950)
                     /locus_tag="NK767_001800"
     CDS             complement(1030..1950)
                     /locus_tag="NK767_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCH6694795.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2063..2815
                     /gene="xth"
                     /locus_tag="NK767_001801"
     CDS             2063..2815
                     /gene="xth"
                     /locus_tag="NK767_001801"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCH6694796.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3679..4212
                     /locus_tag="NK767_001802"
     CDS             3679..4212
                     /locus_tag="NK767_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCH6694797.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4702..5226)
                     /locus_tag="NK767_001803"
     CDS             complement(4702..5226)
                     /locus_tag="NK767_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6694798.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5219..5692)
                     /gene="tsaE"
                     /locus_tag="NK767_001804"
     CDS             complement(5219..5692)
                     /gene="tsaE"
                     /locus_tag="NK767_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCH6694799.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5815..6795)
                     /gene="pta"
                     /locus_tag="NK767_001805"
     CDS             complement(5815..6795)
                     /gene="pta"
                     /locus_tag="NK767_001805"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCH6694800.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6948..7628)
                     /locus_tag="NK767_001806"
     CDS             complement(6948..7628)
                     /locus_tag="NK767_001806"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCH6694801.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7754..8623
                     /locus_tag="NK767_001807"
     CDS             7754..8623
                     /locus_tag="NK767_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCH6694802.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8649..9164
                     /locus_tag="NK767_001808"
     CDS             8649..9164
                     /locus_tag="NK767_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694803.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9211..9804)
                     /locus_tag="NK767_001809"
     CDS             complement(9211..9804)
                     /locus_tag="NK767_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6694804.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9829..10008)
                     /locus_tag="NK767_001810"
     CDS             complement(9829..10008)
                     /locus_tag="NK767_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694805.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10085..10594)
                     /locus_tag="NK767_001811"
     CDS             complement(10085..10594)
                     /locus_tag="NK767_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694806.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10690..11070)
                     /locus_tag="NK767_001812"
     CDS             complement(10690..11070)
                     /locus_tag="NK767_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCH6694807.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11400..12149
                     /locus_tag="NK767_001813"
     CDS             11400..12149
                     /locus_tag="NK767_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HCH6694808.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSRGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKLTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12333..12500)
                     /locus_tag="NK767_001814"
     CDS             complement(12333..12500)
                     /locus_tag="NK767_001814"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694809.1"
                     /translation="MKDIQSGNVQETIDKWKGKHYRFTEAQKQALEYYQKLEESLRFN
                     DEARKYLKELL"
     gene            complement(12516..13610)
                     /locus_tag="NK767_001815"
     CDS             complement(12516..13610)
                     /locus_tag="NK767_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694810.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSK"
     gene            complement(13689..14333)
                     /gene="pcp"
                     /locus_tag="NK767_001816"
     CDS             complement(13689..14333)
                     /gene="pcp"
                     /locus_tag="NK767_001816"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCH6694811.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14532..15476)
                     /locus_tag="NK767_001817"
     CDS             complement(14532..15476)
                     /locus_tag="NK767_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HCH6694812.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15478..16164)
                     /locus_tag="NK767_001818"
     CDS             complement(15478..16164)
                     /locus_tag="NK767_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HCH6694813.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         4653 a         3355 c         2865 g         5304 t
ORIGIN      
        1 tattttaagt tccattactt attaattggc taaacttctg cttgtttctg atattgcttg
       61 tttttctcca atgatccgca cttctgtttc taaagacaca tcaaattttt ctttgattac
      121 ttcttggatg tgcgcaatca attcaatata atctgtcgct gtcgcatgat cgatattgac
      181 gatgaatcca gcatgttttt cagagacttg tgcaccgccc catttcaggc cttgcaatcc
      241 tgcttgttgg atcagctgac ctgtatagtg tcctactggg cgcttaaaga cacttccgca
      301 tgaagggtat tctaatggct gttttgattg acgcaattcg gtcaactcat ccattcttgc
      361 tttgattgct tcataatccc ctgtttgcaa actgaatcga gcttccaata cgatagcacg
      421 taattcttgg actttactgt gacgataaga aaataccatt tcgtcttttg tcaaagtttt
      481 caatgttcca tcttcaagca aaagatccac ttcagcaaaa acatctttga tctctccgcc
      541 atacgcacct gcattcatat aaaccgctcc accgacactt cccgggatgc cgcaagcaaa
      601 ttcaaatcca gtcaatgatt ctgccaaagc tgcataagtc gtatcaatca gtgaggcacc
      661 tgcttccgca atgaccatcg ttcctttgac atgaatctgt ttcatttcag tcagcatgat
      721 gactacaccg cgaatcccgc catcttgaac gatcagattg cttgcatttc ctaataccat
      781 ccaaggtata tcatggattc ggcaataatc gatgatttgc tttacttctt cttgtttttt
      841 cgggaatgca aggacatcag ctggtccacc tgttttcgta aacgtatagt tcatcaatgg
      901 ttcatcaaac aatatagtaa tcgtggctaa gctttcaact atttcttctt tattcactga
      961 ggattttcct ttcttttctc aaacgtcctc attattctat cacgcttaat acaatcttgc
     1021 cagtcttgtt caaagttgat gacggtagtg atctttaagc acttcctcac ataattcatg
     1081 acttcttaca acttcttttt gtttaagcaa ttctgtttcc gatttaccta aacggatctg
     1141 agaaaagaag gcctctacca ttggctcaaa cccgcgtttg actagcgtgt ttgtccagtc
     1201 gccaaattct ttcacttttt ttccatcagc atcttgaatg acaagctggg tcagatcgtt
     1261 tacttcataa gtaccatttt tacttgttac ttgatatttt tctatattag cgccacttgc
     1321 taaatccatc gttaaaatag ccgtttgatt ttgtgtttct aattgtaaaa tggcatattc
     1381 caaaaattcc tttgtttctc ttatctggta ttttgttctc aaaatgggtt catctaataa
     1441 atacacagct gtatcaataa gatgtaaaaa caaatcataa aggacgaact ctgtcgtttc
     1501 ttttgccgct attcgatttt tttgcaggtg gatcaaccgc ttatctggga tacgtttcaa
     1561 ttcttctacc ataggagcaa agcgccggtt gaaaccgatc atcaaaatta tttggttctt
     1621 atcagctaat tcttgcaatt cacgaacttc atttagatgg acactcagtg gtttgtcaat
     1681 ataaacatgg ataccatttt ctagacattt tttggctaat tcataatgta cttttgttgc
     1741 tgcgtggatc atacatgctt cgattttttc ttctaaaagc tcatcgatcg tttcatatag
     1801 atgttggaac ccgtactctt tttgaattct ttcttttgtt tctttatttc ttgttgcaaa
     1861 ataaaaatca agtgttcctt gttttttcgc ataagttggt aaatatgctt tttctgcgat
     1921 attccctaca ccgattacac caatcttcat ctactttcct cctggatcat ttagttactt
     1981 tcatcttaca ccatttttta ctaaaatgag gtacaatagt atcgcaaagg taaggaaatt
     2041 aattcgaagg agtgtgcttt tattgaaatt tatctcttgg aacgtaaacg ggcttcgtgc
     2101 catcgtaaat aaaaattttt tagaagtttt tcatgagtta gacgccgatt ttttctgtct
     2161 gcaggaaacg aaactccaag caggacaaat tgatttagat cttccaggtt attatcaata
     2221 ttggaattac gcggaacgaa aaggctattc cggcacagcg atttttgcta aaaaaccagc
     2281 attgaatgcc acttacggaa tgggtatcga tatccatgat acagaaggcc gtctgattac
     2341 tttggaatat tccgatttct ttcttgtaac ctgttacacg ccaaattctc aaagcgaatt
     2401 aaaacgttta gattaccgat tagaatggga agaagccttt tataattatt tggaaaatct
     2461 aaaaaaacaa aaaccagtca tcgtgtgtgg ggatctaaat gtcgctcatc aaaagattga
     2521 tttgaaaaac tggaaaacga atcaaaaaaa tgctggattc actccagaag aaagagcggc
     2581 tctttctcga ctgttggata acgggtttat cgatacattc cgttattttt atccaaccca
     2641 ggaaggtgtc tactcttggt ggaactaccg attcaattca aggaaaaaca atgctggttg
     2701 gcggatcgat tactttttga cgagcaaaga tttagagcca ggactagctg atgcaaagat
     2761 ccatacggac attatgggca gtgatcattg tccagtagaa ttagatttga aataaagagg
     2821 ttgtgaaaaa gtcgtttagt ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac
     2881 attttgcata aatttggcat aataccgaga aactggcttt tgaacactgt ttatcgagag
     2941 attgtgaaaa agctgtcctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     3001 tatttttcgt ttggttgggc ttattaccgc agatacaagc ttttgaacac cgtttattaa
     3061 gaggttgtga aaaagctgca ctgcatcaag taataagaac agccaaacaa aaatagttcc
     3121 tcatattttt cgtttggttg ggcttattac cgcagatgca agcttttgaa caccgtttat
     3181 taagaggttg tgaaaaagct gcactgcatc aagtaataag aacagccaaa caaaaatagt
     3241 tcctcatatt tttcgtttgg ttggacttat taccgcagat gcaagctttt gaacaccgtt
     3301 tattgagaag ttgtgaaatc agcgtcctga cctgagtatt agagaaacaa atggcaaaat
     3361 agttcctcat attttgctat tttttgagct aataccgagg gtcagctgat tgaacaccgt
     3421 ttagtggaga agttgtgaaa aagtcgccta ctttcgagtc gtaagaaatt tttttgaaaa
     3481 tggttcctca caactttgca taaatttgac ttaataccga gaagtcatga tccccatcta
     3541 actccaaatc acaaggaatg agttcaaaaa ctagcttttc tcaacaaata ttacataaaa
     3601 aattctatgt tttttgttaa atttatttgc ttttttcaaa agtatgttac agtaatttcc
     3661 caaggaggtt ttacagaaat gaattttgtt gccatggatt ttgaaactgc caatcatcag
     3721 ccttatagtg cttgttctct tgcgctagtc atggtaaaaa atagccagat cgttgatgaa
     3781 ttctatacat tgatacagcc tgagactcct tttttctggc gaaatgtcca aattcacgga
     3841 atccatcaag aagacgtacg caatgcgccg aaattcccag aagtctggca aactatccaa
     3901 ccatattttc aaaaaaatcg tttagtcgtt gcacataatg ctgcttttga tacaaaggtc
     3961 ttagcgggat gcttagatta ttatcatctt gaacagccta attatctttc tttatgtaca
     4021 gtgaaaagta gccgtcgcct gtttcctgaa tttccaaatc atcgattgaa cactgtgtgc
     4081 gaaaatttga acatccaatt agatcatcac catgatgcat tggaagatag tcgtgcatgt
     4141 gcggaaatcc tgctttatca agaaaaaaaa ttcgggacag atccattgaa aaaacttgtg
     4201 actataaagt aaaagaatac ttatgaaaca cacaaagagg tcgtgagcct gccgtttcac
     4261 tccgagtcac aaggagcaat ttcaaaaaat agcttctcat gtttattaaa tattgtgact
     4321 ggtgaccgag gagctggcag gagaacaccg tttattggag aggttgtgaa aaagctgttc
     4381 tgcatcaagt aataagaaca gccaaacaaa aatagcttct catatttttc atttggttgg
     4441 acttattacc gcagatgcaa gcttttggac accgtttacc aagaggttgt gaaatcagcg
     4501 ttttgacctg agtattagag aaacaaacgg caaaatagtt cctcatattt tgccgttttt
     4561 tgagctaata ccgaaggtca gctgattgaa caccgtttat tcaaaaaaaa tggactgcga
     4621 cacgactttt gtcacagtcc cttcgtgtgt ttttctgcct tctcaaaagg atgacagttt
     4681 ctcactcttt tcctatttct tctattcaat caaataactc attttatata catctaaaaa
     4741 ttcacctaga tctgttcttg cccctctagg catgaccgct tcgatttgga agcccatttt
     4801 tcggtaaagg tgtacggctc tttcattacg aacttgaacg tcaagctcca atcgaaaaag
     4861 tacacccatt tccttcgccc aactgatgat ttcttctagc ataagagtgc ctagtcccat
     4921 tccccaatat tcctgcagaa tactgatacc gacttctccc acgtgagaca aacgaaattg
     4981 actgtctgcc ttgacagatg ctgttccaat aatcgtccct tcatagattg caagcagcaa
     5041 taaattatta ttgctttcgt atatataatc caactcttct tctaaggttt ctggtggtaa
     5101 caggagttct gcttcatcca aaactaaaaa ttctgtctcc ttattaactt gtcccatcat
     5161 caaaagaatg tcttttgcat ctcctggcac tgcttcccgc aagatgatcc caacttcttc
     5221 actcatgatt ttcagcccat ttttctagaa ttcgctgttt gaaggtttca ctttgctccc
     5281 cgtatgagat tagtttcaca taccgctttt ctaaatctgt atcacttttt tctaaaatca
     5341 gttccaaata atcctccggc agtgcttctt ctaatagatt tccccattca acaacggaaa
     5401 gcccatcacc ttcaaaatat tcatctaacc ctaaatctgc tccgctagct gctacccgat
     5461 aaatatccat atgatacaac ggaatccgac cttgactata ttctctaata atcgtgtatg
     5521 tcggactttt gatcatttgc gttatcccta atccttgggc aattcctttc gtcaacgttg
     5581 ttttacccgc acctagatca ccagtcaata tcagattgtc acctggcatt gcggaatgac
     5641 caattgcctc tgcgaattgt tctgtcattg ttaatcctga tagttcgatc atttttcatc
     5701 ttctcccgct cttctttctt tttgccagta tatcgcgtaa tgaagttttt ttaaagaaaa
     5761 aatgaactag tcaagaacag cagctgctgt ttttggctag ttcgcgtgta tttgttaatc
     5821 aatcaatgtt tgtgctgctg tgataatcga taatttatat acatcttctt catttgctcc
     5881 acgagacaaa tcagaaaccg gtttgttcaa gccttgtaga attggtccga ttgcttcaaa
     5941 gttaccgaaa cgctgagcaa ttttatagcc gatatttcct gactgcaatt ctggaaatac
     6001 aaagactgtt gcttgaccag ctacttctga atctggcgct ttcagttgtg caactgaagg
     6061 aacataagaa gcatcaaatt gcaattcgcc gtcgatcatg tattgtggcg ctagtttttt
     6121 agcgatttta gttgcttcaa ctactttgtc tacttctggc gatttcgctg agccttttgt
     6181 cgagaagctc atcattgcta cttttggttc gatatcgaac aattctgctg tttttgcaga
     6241 atctacggcg atttcagcca attcttgcgc agttggattc acgttgatcg cacaatccga
     6301 aaagacatat ttttcttgat ctcttccacg taccatgatc atcgctccgc ttgtccgact
     6361 gatgccaggc tttgttttga tgatctgaag tgctgggcga acagtgtcgc cagtcgaatg
     6421 gatcgctcca gagaccattc cgtctgcaat ccccatatac gtaagcatcg taccaaagta
     6481 attcacatct tttaaaatct tttctgcttg ttctttggat gcctttccat ttctacgttc
     6541 aacaaaagta tccaccattt catcccactt atcgtaatta tcagggctga tgatcgtaaa
     6601 gctagatgtt ttgattcctc tggcatgcgc tgcatttctt atttcttctt cgttccctac
     6661 taaaatgggt tccacaagtt cttctgcttt caaccgggca gctgcgccta aaatacgagc
     6721 atcagtagct tccgggaaaa cgattttgat cccacggcga acaattttaa atcttaaact
     6781 atcaaacaat tccacaagat gacctccaga cgtttttata tgtattatca tacactattc
     6841 cagaaaaaaa gaacagttta ttttatctgt atcagtcaaa aatgctggaa aaattaagtt
     6901 tttcacatac aaagcgaact gcactataac aggaaagcgc tgttttatta cactgtttca
     6961 ggcagttgcc aatcaatcgg ttcttctcct aacgcaagga gcgcatcatt cgttttcgag
     7021 aagggtttgg aaccaaaaaa gccacgactt gcagataacg gactgggatg tggagatgtt
     7081 atgatgatat ggcgactctt atcgatcatt ttgatttttt cttgtgccgg ttttccccac
     7141 aagataaaca cgattggttt ttcccgttca tttaattttt caatgatcgt gtctgttaat
     7201 ctttcccaac ctttcccgcg atgagaatac gcttgccctt cccgaacggt aagaacggtg
     7261 ttgagtaaaa ggactccctg tttagcccat gacactagat ttccatgttg aacaggtgcg
     7321 attccaagat catcataaag ctctttatag atatttctta acgatggcgg gatctttacg
     7381 ccaggttgca cagaaaaaga taagccatga gcttgatttt ctccatgata aggatcttgt
     7441 cctaaaatca ccactttaac ttcttcataa ggagtcaatt ccaatgcttc atagatatga
     7501 tacatatctg gatggatctt ttgggacgca tattcttttt ttaaaaattc tcttagatgc
     7561 agataataat cttttgaaaa ttctgaggac aaaacttctt gccaactgtt atgaatgatt
     7621 tctttcaaaa actccacctc ctaataaaaa gaataactca cattttcttc aatcacaagg
     7681 gacaatcttc accctcagca cttgattaat atggtaaact aggaacaaga agcagaaagg
     7741 aacgataatc attatgatca aattaattgc atcagatatg gacggcacat tattagatgc
     7801 ccatatgagt gtttctcctg aaaatgccga ggcgatacgt tttgcaaatg atgccggtgt
     7861 tgagttcatg gtcgctactg gtcgaaacta tcaagaagct cgcgcagctt tagatgaggt
     7921 aggaatcgat tgtgcgatga tcactttgaa cggggcacag gtatttgata aagaaggaaa
     7981 ttcattgttt accgtgtcga ttccaaataa tcaagcaatc tctgttttag atattctaga
     8041 tgctaacggt atttactatg aagtagcaac caacgatggt ctttattctg aaaatcaagc
     8101 gaaacgaatt gaaagttttg cttccatggt ggctacccat ttacctcacc ttacgtataa
     8161 aatggcgatt gccatggcct cagctaagct ggagttattg cacatcacat acgtggacag
     8221 tattcgtgct attttggaag aaaagaatct ggaagtttta aaaattattt gttttcatac
     8281 agaagggcca gctgtattag gtccagttgg aaagcagatt tctcttttag gcgatctagc
     8341 cgttacttcc tctggtcaaa acaatatcga agtgaaccat aaaaacgctc aaaaaggaat
     8401 tgctgttgcc catgtcgctc acgagcgtgg gatttcttta gaagaagtca tgacgattgg
     8461 cgataacttc aatgatgtca gcatgctgca agtagcagga gtcagtttcg caatgggaaa
     8521 tgcagaaatc gaagtaaaag attatgccaa atatacaacg gacacaaacc ttgaatctgg
     8581 cgtaggaaaa gccatattgc gcgcgattgc agaagacttg tagttttatc tagaaaagag
     8641 ggatatagat gtcagaattt ttcattcagg aaaagcagct tggacgtgca actcggacaa
     8701 ttgtcaaaaa cgaaaaagga cattccctgt ttttgatggt tggtcgctgg ggaacaaagg
     8761 gcgatgcatt gtctctttat gccatgaatg gcgatctcgt tgctcacatc aagcagataa
     8821 gcctgacatt cggtacgcgt tttgagctgt ataaagagtt cgaaaaggtg ggaaccatgc
     8881 agaaaatatt caattggccc ggtgactttt attatattcg tagacttcac tggacagtcc
     8941 aaggtgatat ctataatcat caatatcaaa tccatcattt caatcagcaa atcatgaaaa
     9001 tggataaagc aacacttttt tcaggagatt actatgtatt ggatgtctca aatgatgaag
     9061 atgctccagt ttgtatttgt attgctgcgg ttttagatta ctggctctac cgtaaagaca
     9121 aaagtcgcga taagcctgtt tcttaccgac ttaacttaag ttaaaaagat gtacctgaaa
     9181 tgcggaaata cgcatttcag gtacatcttt ttagatattc ttttgcatat gatcataaag
     9241 atgaccactg atcatccgct gaccgacaac tttgaagtca tggcgttcgt ataatttttt
     9301 agcctttgga tttttttcat ccacacttag accgatgacg gagcgattag ctttcttcgc
     9361 caatttaggt aatgcttcta ataatcgcga accgatgcct tgtcctctaa aatcttctga
     9421 tacactgata gagtccaaat accattcgtt gggaaacgtt tctttatcaa tgaacaaacg
     9481 tgtagacgca tctagctgat atttctctaa gataggtacc agtggctgat cgatgattga
     9541 ttcttcttca tctgtataac cgtaagcaac tcctgcaact ttaccgtcta tttcttctac
     9601 gatacctcgt gaataactgt aacgataatt tggatcagtg attgcttctt ctaaaatctc
     9661 taaagtcttt tgttcccctg tctgcttcaa aaatggcagt tccatatctt ttagaatcac
     9721 taagatcaat ggggcgattt ctggagcatc ttcttttctc gcaaaacgaa tcatagtttc
     9781 ctctcctttt ctccattcta acatgcagaa aaagaaatcg caaaatcgtt attttaaaaa
     9841 atcttcaatc ttttcagtaa tgaaataacc atgagtaacg attcttgccg cttcagttcg
     9901 gcaataaggg cagacgactg gcatttcttt atcattatgt tcgactacat tattgacttc
     9961 ttcgtatgca aatttttttc cgcaattagg gcatttcatt ttttccatgt tatctcaact
    10021 tcctttcatc tttactctct tagcttactc ttatattgta aaaaagagaa gcaatatcct
    10081 tcttctatgc agatatccat acctgacggt cctgttctcc atctattaat cggactttat
    10141 aataaggacg ttcatctttg actagaagtg tccactcttt cacttttgct tttttcccgt
    10201 agtagctgac tgcttccttc atcgctgctt gcggttgttt gaactcttcc gttttttcta
    10261 tatcaaaaag atgttctgcg gccttttccg tgtcatttcg tacggttgtt ttcccagtgg
    10321 atggattgac tgctatgact tgtccttcac aaaaaaactg gtattcatat gttgcattcg
    10381 taggattctc caaatctttt tcaactgcaa aatgaatagc agatatgtta tgttcttttg
    10441 tttcgttttt tacgattgct gctgcttttt tcaatgagat ccgatattcc tggtcactaa
    10501 tatcatccat gtgaaaagcg gcattgagca gtcccgtcat accggtaatc cctattacga
    10561 tgagggcagc aacaccaaac atcatttttt tcattcttca ttctcctgtt tctttttttt
    10621 aaagaatatc aagaaatgaa cgaatgaaaa atcgaatgaa acgaaaaaaa agtgacaatt
    10681 ttgttatctt tattcgtgca gttctaatgg caatccgtct ggatcaaaga aaaacgtcat
    10741 tttttttcct gtaaaggtat cttctctaat cggttctgat tcgatcccta aggactgcaa
    10801 ttctgcaatt gtttcttcaa tatcgtctac tttgaaagca agatgacgca agccacaagc
    10861 ttctggatag tttggtcgtt gtggcgcatt agcaattcca aaaatctcca attcagcact
    10921 tcctagtttc agatctaatt tatagtcatc tcgttctttg cggtaatttt ctctgattac
    10981 ttcaaatcct aatttattaa cataaaaatc acgtgatttc tgataatctg aaacaatgat
    11041 ggctatatga tggatcgttg taaggttcat atgagcactc ctattcttcg tttaagctcg
    11101 gatctatatt aatctccagt tccattgtaa atgtgtcaga ttgaaaaaaa tagctgtttt
    11161 tcctttttat aagaaaaaag gcaaaaaaaa cagaaagtag gtcctccaag acactcttcc
    11221 tgttcccaat ataataaatg aaagtaagaa attatatttt caagaaaaca aacagaaaat
    11281 tatcataaca caaattattt ataattgaaa gactagtttt ttttcaagaa aagcatacaa
    11341 tagattgtgt tagtaaatac agaacacact acatttagtg tcttaaggag tcctcataca
    11401 tgaagtcata ttttcagttt ttagctcatc agctaaaagg ctggccgcaa caaaattatt
    11461 atcttttctt tttcagtcgt ggctgccagg tcatgactct tgtcaatagt cccatcactt
    11521 ggctgtccgt tattacattt atcggtacca cgcttggtgt attgtgtatt ttatccatca
    11581 atgcggcaaa atcagtcaac ggtattttag gtattttgtc tgcgatttgt tttattatcg
    11641 ttgggttttc agcaaagaac tatttaagta tcggtgaaca gattgcttat atgatcacat
    11701 tggatatccc cgtattgtta agtaaagaat ggaatcataa tatggcttca aaaatccgaa
    11761 aattaactgg aaaaagctgg tttatcgcaa tcatcagtac tttccttgtc tatcttgttt
    11821 caggattcct gattggtcgt tatacagatg atcctcggcc gtgggtagat gccattgctt
    11881 tttctattag cttgactgct ggtatcatca gtttcttgcg ttacaacaac caatattact
    11941 ggtggttagc ttcagggctt gctcaaatgg tcctttggta tatttcattt acgcatggat
    12001 ctgcaagttt ggcgatgttc gtgaacagct ctgtttactt gctgaatgat gtccttgcat
    12061 ttaccgtttc accttggtat aaccaaaaag aacgtgaacg tatgcaaaaa atggaagaag
    12121 aatactacaa cgcacaacaa cacgcataaa aaaactgagc gtgggacaaa agtaaagaac
    12181 acttttgtct cacgctctaa acacgtataa acggcggaat cagaagcaac tccttcggaa
    12241 ataagctgaa attcacaaaa atttgaaaag caattttcgt gaatttcttc ttatttcttg
    12301 gagttaaacg cttctgtccc agcctctatt ttttatagta attccttcag atactttctt
    12361 gcctcatcat tgaaacgcag tgattcttct aacttctgat aatattccaa cgcttgtttc
    12421 tgagcttcag taaaacgata atgttttcct ttccatttgt cgattgtctc ttgcacattt
    12481 ccagattgga tatctttcac tactagttgc catttttatt ttgagactgt acggtctttc
    12541 ttatcagcag attgtatagc ttctcgttct ttacgcaata cttttaaaaa accatttctg
    12601 actatccgag gaatgctgtc gaagaaccct tctgtaaaac catagcgaca atattgcttc
    12661 caaataaata gatagatttt tggttggatg aatcgttggt ttttcgcaaa atattccatc
    12721 tgagcagtga cttctggtgt tcgttgacgt tttgtctttc tcaacacgga aataaatgtt
    12781 tcaaaataag cattaggatg tactggcact tcttcatata cgatggattg ccaatcttta
    12841 cgtaaaaatg cgcgatagtt gatcaagaat tcttctacat cctttactaa ttcttctgca
    12901 aagtcgtcaa atagttcttt ttttatctgt cgcttttttg actgcccatt gatgtgttca
    12961 cttattttct tgatttcatt ttccaatgct tgataatccg ctatgtaaat cggcatttct
    13021 acttggcgaa attctgcgag ccgctgagct aattttatat ccggtaaata tggctgtttc
    13081 atccgacgat tttggtacaa gacaaaacag tatttttgcc atagtccttc aggaaaatcg
    13141 atattcttct gcttaagcca taataattcg tctaaggcca gatcaacttt tgtcattccc
    13201 tgatggatcg aagctgccac tgtcggagag acatgcaggt gatcggcaaa gtggttgatg
    13261 cctagtttta ggatctttcc ggtttttggc gatttaacga tgtattgcac ttttaattct
    13321 ctaccacact cacaatacaa tttttgctgg cttcgttttg ttcgaaaaaa cggattgatc
    13381 ttgaagtcag aaaacaccca atctgttgca gctaaataat gttcttccat aaaacgagag
    13441 ctgattagat atcggcggtg cttatcgact agttcccgct gttttttcgt taattttgca
    13501 tagatctccc aacgttgact gcttgtgtat tgtttagaaa ttttgatggg atagcttttg
    13561 ctgatttctt ttggaaacca ttcttcaaag acggattgcc attcttccac ggctgtttct
    13621 ttcctttcta attacaaaaa gctggctact aacataacgt tagcaaccag ccagtgtttc
    13681 ttgctaaatt agtgagttgt tccggcattt ccggaaatat cttctttatt ttctactgct
    13741 gcttcaactg cataagtcaa agaagaagca atgtcttcta aagacataga aggcgtaccc
    13801 actggacgat cgatcacttg atcgggtgag aatggcacgt ggatgaaccc acctcgaatt
    13861 gccgggaatt gtgtttcgat caaatgaagc aaacgataca tgatatcatt gcagacaaat
    13921 gttcctgctg tataagagat gtacgctggt agaccatgat cttgtacatt ttttgtcatt
    13981 gctttgattg gcaatgaggt aaagtaagcg gctggaccgt tttcttctaa agccgtatcg
    14041 aatggctgat ttccttcatt atcagggatt cttgcttctg ctagattgat tgcgacacgt
    14101 tcgaacgaga ctgctgaccg cccacctgct tgtccgacac agatcacgac atctggttgg
    14161 tgctgttgga tggcttcttt gactacttga ctgctttttt cgaatacggt cggtacttcc
    14221 actttgatga tttcagcacc gctgatttca tctggcattt tttttactgc ttcataggct
    14281 ggattcactt tgtccccacc aaatggatca aaacctgtta ctagtacttt catctgttgt
    14341 tcctccgtcc gattgattta acatatggtt atcttttatc ataccatttt ccttgaacag
    14401 cgtaaataaa ggaaacaata gtttaaataa cataacagat caaacttaat atattcttat
    14461 aaaaaaatag tgttccacta caatgttaga agaggttgcg acaattattc tgtcacaacc
    14521 cctctcttct attatttaaa tagaatcata taagcaattt ggaaaactag cataaataac
    14581 gcaataaaca actggttttt gatcactccg tatttgtctt tcatctccag catcgctaca
    14641 ggcacgatat tgaagttagc agccatcggt gtcagcaggg tcccacaaaa accgcaagtc
    14701 aatgcaacca ttcctattaa cgctggattc gctccatgac taaatacgaa tggtgcaccg
    14761 atcccaaccg tcatcacggt gatggccgca aatgcattcc ccatgattac cgtaaatatc
    14821 accatgccaa gagcgtaaat gatgatgccg atgttcacat ttccttctgg aacgatcgta
    14881 cctactgcat tggaaatcac tgtcccaact ccagcgcttg taaataccgc acctagagaa
    14941 gctaaaagca ttggcaacat gcttaacggc cctactgtgc ccagcatatc tgctgcatca
    15001 tctaaaaatg tacttggtcg attatctcga gaaaagaaca tcaagattag catggctgca
    15061 aaaacaccga cgcccacgcc gactaaagca cctaaattgg taaatagagc aaacaagatc
    15121 gcaaagacac cgatactcaa agctggaata aaaattttca ttcctagttt gtctgacatt
    15181 ttttccatat attcttttga tggaagccgg ctttctcctt tgctgacttt cttagcaatt
    15241 gctggtatgg tcatcgcaaa gatcaagcca ccgttcacga ttgccggaag ccatcttcct
    15301 cctgcaataa ctacccctaa gaccgtccag aaaaaggctg acccataacg atgaggattg
    15361 gttttatctg ttaaatttct tactccagtg taaatcaata cgagtcccat cacgatataa
    15421 aggatctcca gtaatttatc gcctaataaa atatcaggat ttgtaaaaaa gtccatctta
    15481 ttgccccttt cctggataat atttcttcgc taatttcttt tctttaagat agaagtagac
    15541 acttgccaca actaaagccg tgatagccac tggaacttcc actaaagcaa gctgtcccaa
    15601 tgttacgtca tatcctagat ctttcagtgt tgattggact agcaacgccc ctgctcctcc
    15661 gatgaatagt acttggccaa agaaccagca gacattttcc atagctgaag ccataccttt
    15721 tagttcttct tcatgcttag gatggacttc taaccctttt gattcgatgg tccccaatgc
    15781 catcggtaaa atgattggac gaacaaatcc agcaactcca ccaaaactca cgttaaaagc
    15841 cgcaaagatc attcgaaaga tcccataaat atctacaatc ataccagcag atactttctt
    15901 gaatcgcttg atcaaggaag ccgctgattc tttcagacca tttctttcaa gcgttcctgt
    15961 tgccaacata atgatgataa aaatcgccat cccgcgattg tttacaaaac ttgtacctaa
    16021 aagttctaac attccttgaa tgccaagacc gccagtcaaa gctgtaacaa ctaaagcaac
    16081 cattacgatc aatatcgagt ctaatttaaa tgcaaacccg agaataacaa tcaagacgcc
    16141 taaaaggcta agataatttt ccatatattt tcctcca
//
LOCUS       DAHUVV010000057        16061 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.058, whole genome shotgun sequence.
ACCESSION   DAHUVV010000057 DAHUVV010000000
VERSION     DAHUVV010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.058"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..432)
                     /locus_tag="NK767_001819"
     CDS             complement(<1..432)
                     /locus_tag="NK767_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCH6694814.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFL"
     gene            complement(562..1272)
                     /gene="budA"
                     /locus_tag="NK767_001820"
     CDS             complement(562..1272)
                     /gene="budA"
                     /locus_tag="NK767_001820"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCH6694815.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1285..2949)
                     /gene="alsS"
                     /locus_tag="NK767_001821"
     CDS             complement(1285..2949)
                     /gene="alsS"
                     /locus_tag="NK767_001821"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCH6694816.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3154..3816)
                     /locus_tag="NK767_001822"
     CDS             complement(3154..3816)
                     /locus_tag="NK767_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCH6694817.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3826..4641)
                     /locus_tag="NK767_001823"
     CDS             complement(3826..4641)
                     /locus_tag="NK767_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6694818.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4903..5346)
                     /locus_tag="NK767_001824"
     CDS             complement(4903..5346)
                     /locus_tag="NK767_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HCH6694819.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5480..5818)
                     /locus_tag="NK767_001825"
     CDS             complement(5480..5818)
                     /locus_tag="NK767_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCH6694820.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5806..6183)
                     /locus_tag="NK767_001826"
     CDS             complement(5806..6183)
                     /locus_tag="NK767_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCH6694821.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6407..7600
                     /locus_tag="NK767_001827"
     CDS             6407..7600
                     /locus_tag="NK767_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6694822.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7766..8188)
                     /locus_tag="NK767_001828"
     CDS             complement(7766..8188)
                     /locus_tag="NK767_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6694823.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8583..8777
                     /locus_tag="NK767_001829"
     CDS             8583..8777
                     /locus_tag="NK767_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694824.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            8774..9268
                     /locus_tag="NK767_001830"
     CDS             8774..9268
                     /locus_tag="NK767_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694825.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(9430..10602)
                     /locus_tag="NK767_001831"
     CDS             complement(9430..10602)
                     /locus_tag="NK767_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCH6694826.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10808..11623)
                     /locus_tag="NK767_001832"
     CDS             complement(10808..11623)
                     /locus_tag="NK767_001832"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCH6694827.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(11773..12219)
                     /locus_tag="NK767_001833"
     CDS             complement(11773..12219)
                     /locus_tag="NK767_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCH6694828.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(12216..13220)
                     /locus_tag="NK767_001834"
     CDS             complement(12216..13220)
                     /locus_tag="NK767_001834"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCH6694829.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(13260..13589)
                     /locus_tag="NK767_001835"
     CDS             complement(13260..13589)
                     /locus_tag="NK767_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCH6694830.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(13715..16042)
                     /locus_tag="NK767_001836"
     CDS             complement(13715..16042)
                     /locus_tag="NK767_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCH6694831.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4581 a         3225 c         2859 g         5396 t
ORIGIN      
        1 tagaaatgct tgatcgattt catcataccc tttcgttact ccgcaatcga tatctaaaac
       61 accgattaaa ttaccttctt ttaccattgg cactacgatt tctgattgtg cggctgaatc
      121 gcaggaaatg taatttgcat gggttttgac atcatcaacg atcaatgttt cttgtttttc
      181 agctgattct ccacaaactc cttttcccat cttgatacgt gtgcaagaaa ctcgcccttg
      241 gaacggtcct aaaatcaatt cattaccatc gaataaataa tatcctgcaa agacagtctc
      301 aggcaaagtc tcattcaaca atgcagatga atttgctaaa tttgctaacc agttatcttc
      361 tgcttctaat aatccttgtt gctgtaataa taaaatttcg tatgcatctt ttttctgttt
      421 ttctgtccac atgataaaaa ccgctccttt ttgatctatg gagaagatta tagctcagtt
      481 tttttgtaaa cgctaggttt cctcctcaaa aaaacgagat acgtcaggtt aatcaccttg
      541 aacgcatctc gctgtctttt tctattctgc ttgttggatt tctgctgcta aatcagcata
      601 atcaatcgtt tcgtgaacaa aagtcggatc atcaactgga aaatgctgta caagggacga
      661 gatgcggcta atctcaactt ttccttcagc cacttcaaaa tccattacat gtccgccaaa
      721 tgtatgtgta tcatcgataa aatgcaagtg aaatcccgct gcagcaactc cttggaataa
      781 ttcaggcgta aagaacccga ctaatgttcc ttggacattg ctttgttgga attctggttg
      841 agactctgaa atttttgcta gtcttctgta aggtttttct tgcttcggca tgatacggat
      901 gtgcatattt ttgaataatc cagttatctt cactgccaaa aataggtttt ccccggcttc
      961 tcttactaat atgtcttttt tcaattgttc tccatctgca gtttttgaga cagaaaaact
     1021 agtatctggc gtaaaatctg taattgctgc atagggggta agttcacttc catctaattg
     1081 ggcaactgag ccatctgatc ttccttgata agctacgcca tctaaaaaga tgacttcacc
     1141 atcaagccca tgcaatgttc ccaatcccag agttcctttt tctaatatat gagcaatcgt
     1201 ttctgttcca tccatcaagc cagccatcaa agctcctaat gtcccgtgct ggtataagat
     1261 tttttcagtc atgtcgacac ttccttaata gaattgatct ggtaagattg tttctcccaa
     1321 tttctcatta tcacggtaat caatcggaat atcaataatt acgggtcctt ctgtcgcaaa
     1381 tccttcttct aaagcttttt ctaattcctc tacacttgta gctcggattc ctttagcgcc
     1441 aaaagcttct gcatatttca caaaatcaac tggaccaaag tctacacctg acgcccgctg
     1501 atatttcatt ttttcttgga actctaccat attgtagtgt ccatcattcc aaatcaaatg
     1561 aacgatattc aattttttac gtaccgccgt ttctaaatcc tgggcagaga ataaaaatcc
     1621 cccatcgccg gaaactgata cgatttgtgt atttggacgc accaatgcag cagaaatcgc
     1681 ccatggcaat gcaacaccaa gcgtttgcat accattactg aataacaaat gacgaggttc
     1741 ataacttcta aagtggcgtg ccatccaaat ataatgacta cctacatcta cggtaacagt
     1801 catatcatct gttacttttg actgaagcgt gttaataact tctaaaggat gcaagatacc
     1861 tgcttctcct tttgattgaa caatgtcccg ctccgttaat tctgcttgaa gagatgctaa
     1921 atattctttt gcatcttctg acacttgttg aggctctaat gaacctgtca ataaatcaag
     1981 agtggctgag atatcaccga tcaattctct ttcaggctgc ataaaaggat cgatttcagc
     2041 tggcgcttca tcgatcacaa tgatccgcgc atctttttcc gcattccagt ttcttgcttc
     2101 atattcgatt ggatcatagc caatagcaat aactagatca cttcgtttca aaagcatatc
     2161 tcctggttgg tttctaaata aacccacacg accaaagaaa tgcgcttcta attcacgtga
     2221 gatcactcca gctgcttgga atgtctctac aacaggcaat gctgtctttc caactaattg
     2281 cctgatggct aacgtttctt tttcacttga tcctcgcata cctactaata gtaccggtag
     2341 ttttgcttct ctaatgcgtt cagctaggta acggatatca tctgcagaag cagaacctaa
     2401 tttaggatcg ctcaatggct tgatgacatt cccttggaca ggtgcatcca ctacatcttg
     2461 cgggatactg ataaagctcg cgcctttttt tgaactctta gccatacgat aagcattggc
     2521 aatcacttct gaaattgttt ctggatcttg gatttctgca ctgtattttg tgattggctg
     2581 gaacagtgct gcattatcca tgctttgatg ggtcaacttc aatagatcgc ttcgttttac
     2641 ttgtccggca attgctaaaa ctgggtcacc ttctgctgtc gctgttacca aacctgtagc
     2701 taagttacta gcaccaggac cacttgttgc gataactact cctggctctc ctgtaatacg
     2761 tccgattgct tgtgccataa atgcagcatt ttgttcgtga cgagtcacga ttaattcagg
     2821 accttgatcc tctaattcat taaatacacc atcgattttt gcacctggta taccgaatac
     2881 ataatcaaca tgatgattaa tcaaactttc tactacagat ttcgaacctt gtttcatttt
     2941 ttcgtccatt gaacatcgtc ctttttatgc tttattttta attattagtt agaaataatc
     3001 acaaataatt gatacagttt ctaaacctgt ttcattcaaa agtatagcac gttttatttc
     3061 atttgtttag ccatatgttt caaaaaatga aaaagaatga cagtttttac ataaaaactg
     3121 tcattcttaa ttgaaagttt caatttataa agtctacaga tccatagttc tcatcaattt
     3181 acgattgttg aagtaaatca aaccaaatac gatagaagga cctagtagaa tacaccattg
     3241 gggctttacg aaaaagaaga gaaacaaact tgtcaatagt aaaaaatcta gtaaactcac
     3301 ataccatttt ttcaacgtat agtagaacgc tatccgtaga acttgatttg gtttcgacgc
     3361 tggatttctc acctgaaaat accagtagtt gatgctaaag gaaactgata aacaagcaag
     3421 taaaacaaac aacggaatca accattttcc ataagaaaaa cgtataaaaa agaaacaatc
     3481 aaaaatcatt atcacactaa ctatccaagc aaatagccaa taaaaaaatc cctttagcca
     3541 aaattggcga aacgtcttta tgaacaaacg tactggtgaa aaactttttt cttttgctaa
     3601 ttcttctgtc atacctatca tcacgatgca acctggacca ataaaaagta aagaaaaaat
     3661 aaaggaaaag acattccttc catctatcga caaaaggaac gcagctgcta tatacaagat
     3721 attgacacac caaaaacaaa ggctacttgc caatgattga tacaaccaat aaaagaattc
     3781 catataaaca tttttgtcaa aacgctgctc tttcataatc acctcctatt ccttcattcc
     3841 tgatgaagcg atcccttcta caaaatattt ttgcatcgct aaaaatataa aagcaacagg
     3901 aagaacagaa acaactgagg cagccatgat taatgcgtac tctgcatcaa atagcccaat
     3961 aaacattttc aaccctaact ggattgtttt attttccgta gaagacaaat aaatgaatgg
     4021 ccccatataa tcgttccatg tattaacgaa tgtgatgatc gtcaaactgg caatcgccgg
     4081 ttttgtaaga ggtaaaatga tcttgcgata gattccccac tctgataagc catcgatccg
     4141 cgcactctca cataaagaat ctgggatact actgtagtat tgcttcaaca agaacacacc
     4201 aaaagcattg aacgcttgta atagcaccaa cgaccacaac gtatatgtta aacccacttt
     4261 tctcatcata atgaactgcg ggatcatata agactgccaa gggacagcaa tcgtgccgat
     4321 atatgctaaa aataaaatat cgcgcccttt aaaatttagt ttagaaaacc cgtaagcagc
     4381 aaaactagaa gtaaacaact gaatcaacgt gatcacaacg cttaaaaaag ctgtattctt
     4441 aaaaaatgtc actaaaggaa tacgagacca aataaccaga taattctcta aatgccaagt
     4501 tttagggatc cattgaatcg gaatcgtgaa cacttcatta tttgctttga atgaagcaga
     4561 aaccatccat aaaaaaggaa tgatcgtcaa taaagctaat acaatcagca gaatcatacc
     4621 caaccattga ataaagggca tgttttttga acgaattttt gctttttttc tttcatttcg
     4681 aacacctcca aagttataat cttcatttct ttgtaatgat tacaatccag aacaacggtt
     4741 attatagctg attcatttcc gttcttctta tgtttgtaag cgcttattta acaagttatg
     4801 taaatctctt tatttttttt gatttaaaaa tgaaaaaata caaaagcaag actgtcatac
     4861 gtctcgcttt tgtattgaaa tttcttcgat tagagatact tattactgtt caaaagcttc
     4921 tttgaaacgg tgaatcaatt cattgactac aggtgataaa atccgattct ttttccagac
     4981 tagcatgcag ttcgtttcaa tcattgggtc aagcggaacg aatgtcactc gctttgaaag
     5041 atgacttaca gcaccttcta tgactaaagc agcacctacc tgattctcaa ttagcgcaaa
     5101 tacattgaaa atcaaattgt acgttcctgt gatcagcaac tgctcctttt ttatgcctaa
     5161 ccagtcagca aacattcgct gtatttctgg acgctttgag atcaaaagag gtacattcgc
     5221 aaagtcttct tttgttatca cgtctttttt tgctaaaaca gaattttcta aaatgagcaa
     5281 tccccatttt tcctttcttg gtaacaaaat cgtttcgtat ttctctgtat ctactggatc
     5341 tagcaaaatc gctaaatcta acaggctttt ttctagtcac tccgtgatat catcacttgt
     5401 tccgctgtgg atatgaaatt gaacgctggg ataatcacgg atcaactttt ctagtaatag
     5461 tgccaatgta ttcgaattat cagcttccac acagccgatc gtaaatcggc cgctgaataa
     5521 ttgttttttt ctgttctcga attcttgagt ggtttgattc gttaaatcta aaatttcttc
     5581 tgctcggctt ttttagaaaa taacctgctt ctgtcaagat cattttgcgg ttttcgcgta
     5641 caaacagttc tgttcctagt tcctcttcta actccttcag ttgtcggctt aaggttggtt
     5701 gtgtaagatg tagaacttct gcggcttgag agattgttcc ttcttctgca attgtccaaa
     5761 aatattttag taagcgtaac tccatagcga tgctccttga tttttttact tgatacattc
     5821 cattttattt ctagataaca cacgatcata tttcttgatt tttccttgta aacgttccaa
     5881 acactcttgc atttcagcaa tttctttcgt caaaagttca ctttcttcaa tcaaaagctt
     5941 acgccgctca aatacagttg cttctccttc gctagatagg cgaatatatt caatcaaaga
     6001 ctcgatcgat aatccggcat ttcgcataca gatcgtaaag tcgacccatt ttaggtcttc
     6061 ttctgtataa tcacggattc cgccttttgt ccgattaact ggtggaatca acccgattcg
     6121 ttcataatag cgcaatgtat cggccgaaat gcccttttct tcactgactt gtttgatatt
     6181 catctgatca ctctttctct taatttcttg ccactttact tatgtatttt atttattcta
     6241 caactttaag tgtactccaa gtcaatactc atttctatta aaactaagct ggattatcta
     6301 tatcggttgt ttgtgtatta tttcaaataa aaaagtcttg tttttttatt cttttttgga
     6361 tatactttta acaatttagg gaaattttag gaaaaggggt ataaaaatga caacgtatca
     6421 gcgaaaggta ttattatcga catcggcggg aattgcttta gagaatatgg atattatgtt
     6481 tttagctttt tcattatctt caatgattac ttcttttcat attagtggta cgcaagcggg
     6541 tttcatttca acgatcacta atttggggat gctcattgga ggtatttttt ttggcatcat
     6601 agctgataaa tatggacgtg tgaaagtatt ttctcaaacg gtccttctgt tttcgattgc
     6661 ttctttattg atgtattttg cttcaaacat ctacttggtt tatctctttc gctttatcgc
     6721 aggtgtagga gcgggaggag aatacggggc gtgtatgtcc ttgatttccg aaagtttttc
     6781 aaaaaaacaa attggacgag cttcttcggt tgctggtatt ggtgcgcagg tcggtgcggc
     6841 tttggctgcg attttagctg ctgtgatcat tccaacgttt ggttggaaga tgttgtatgt
     6901 cgtaggtgta ctgccggtgt tgatggtctt gttcattcgt cgcggcttga ctgaaccaaa
     6961 ggaattcaaa tctgtacaat ctgccgaaaa gaaaaccaaa cttagcagac tgtttgaatc
     7021 aaaacatctt gcttggcaaa catgtggcct tagtatcatg gtaactgtcc aaattgccgg
     7081 ctattttggg ttaatgaatt ggctgccttc aattatgcaa aatcgattag ggctttcggt
     7141 ttctgggtct tcattgtgga tggtcagtac tattttaggc atgtctttgg ggatgatgac
     7201 ttttggcata atcatggata aattggggcc taaactagcc ttttctttgt ttttaatttg
     7261 ttcatcctta tctgtctatc tacttgtgtt agcccatagc cagtggagtt tggtagcagc
     7321 cgctgtgata gtcggctact ttatcaatgg catgtatggt ggatatggcg cagtgattag
     7381 tagtttgtat ccaacagaaa tccgcgcgac ggcgaataat ttcattatga accttggacg
     7441 agctgttggt ggtttttctt ctatcgtcat cggtttcttg atggatcatt atagtttaac
     7501 tgccgtaatc ttgtttttga gcggtattta cctagtcagt ctgctggtct tgacaactct
     7561 tactggtgtt acagcattaa gggaaagctt gcagcattga tagttgagtc tgtaaatgac
     7621 aagaaagagg tgtggcaaaa gcttgcatct gcggtaataa gaccagccaa atcaaaaaca
     7681 tgagacactg tttccgtttg gctggtcgta ttacttgatg cagggcagct ttttcaactg
     7741 ctaatttcct tctatctggc ggcgactatt ctttgacgtc cgtcatttca aagtacttta
     7801 ctaataactt agcataacga aaaactgtgg atacactcgt atattgagcg tctgcataat
     7861 cttccaaatt tttctcagga tgttgataca tgaattcaat caaatgataa ttatatgatt
     7921 ttctgtagaa atgaagataa ataatgtaga ttgggaagtt ctcctgcttt tccatagaaa
     7981 agcgccgatt attctcattc ttcaaaatga ccttatcagg atatttttgg aacaattctt
     8041 ctaaatctgt tagataattc aagatagact tacttgatac cttcaattcg ttttgtagtt
     8101 cttctattga acaccaacgt tctgtttcaa gcagtaaatg aatcatttta attttctttt
     8161 gagaaacttt tgttaaaaaa gtctgcatat tttctcctcc tgtatctact ttactatact
     8221 acatacaaga aaaaaaacgg atacaaatat catattgtca caccgtcaat agtgcataaa
     8281 cctgttatta tttctctttt tttcctaagt attctattga tatgaatcat ttaatcgttt
     8341 attatataaa tgtcaataat ctaataataa aataacgtac atattttatt attgcatact
     8401 taataacaga actatagcaa aaaatcccgt gtatcaacac gggattagat taagtttcct
     8461 caatcactat tcataacaca attattataa caagtaagta aacaattgaa aaggacattt
     8521 attgaaaacg gttgtttttt acttaaggag gaaatttagc gaaaaaatta gttgcattgt
     8581 ttttggtcgc aggaagtttg ttgagtgggt gtggtgctac tacgtctact actcaaagca
     8641 caagttcaga tcaggcttcc agcagcagta ccacgatctc cgaaagtacg gcagattcat
     8701 cgaaagaaca agaacaaatt gaaaagaaaa aagaagagaa acaaaaagcg gaaaaagctg
     8761 cactagaaaa aaagtgacag aagcggaatc tgctttgtca caagctgaag caaatcctac
     8821 aaaagaaaac tatgcaactg cttcagcagc tatccaagcg ctacctgaaa acaaacaaga
     8881 gttgacaagc cgtcttgctg caattgattc cacaatcaaa gcaaaagaag cagaagaaga
     8941 acggaaaaag caagaagaaa ttgcacgaca acaagcagaa caagcgcaac aagaagcaat
     9001 cgcacagcaa caagcagaac aggcgaggca agaagcaatc gcacagcaac aagcagaaca
     9061 ggcgcgacag caatcagcag ctgaacaggc ctctcagcag gcacaagccg cagctcctga
     9121 gcaaaataat gaacaaactg tctatgtcac accgaccggt tcaaaatacc atacacataa
     9181 atgtggaaat ggtacgtata cccctgccac tttaagcgaa gctcaagctc gtggtttaac
     9241 cccttgctcc aaatgttatg gaaactaatt caaaagttat actataataa taaaattctt
     9301 ctgaaacata aaaaaataga agatggcgtt ccagatagta aaccaaaaat aacagtttac
     9361 acgtccattt cgtcagcttc tctctttttt catttagtct gttccctctg cttttttgga
     9421 catcttcatt cattctttct caatcaaata gtacacctct ttttcttttc gcagtttata
     9481 tcttcctttt ttcaatttca ttgaaaactg tttcgaatgt ttatgtttta catggatttt
     9541 aagcaggagc tcctcatgtt gcaagcgata gttcctgcct tttaaatcag aaaatctcac
     9601 tagtccgtat tcatcagaag ctttcacaaa tggaactttt tctctagtaa tatagtgttt
     9661 gccgttgagc gtttttacag aaaagaccat tccttcgatt ggtcgtccgt ttgtgttctt
     9721 tacataaaaa gataaaggat agtttcgctt agcgatggcc aaagaatcta aataagcgat
     9781 gagtagccaa actaagccga ttgatccaag tagtataccg ataaaccaaa taaaaaattt
     9841 ccattttttc cattgatcaa ttccttggat cgcttttttt gcctcttttg cttgataagg
     9901 aatacgatgc cccgtcacaa gcagtcgatg tgtattgacc atataaggag tacaggtaag
     9961 caaagtcacg agatctctgc ctttttggat acccaaagaa tctacttcat caggtaagac
    10021 cacttggatt ttttctactt gatacgccaa tgtctttcca ttgatctgga tgaaaaactg
    10081 gtctcctttt ttcaacttag gcaaatctgt aaataatttt gcttctggga gtccacgatg
    10141 cccagaaaga acggcatggg tgtttttccc gccagtcgga taagaacttt cttccaataa
    10201 tgacgttcct ttttgcaaaa atatttccgt tgtctgatcg aatataggta agctgacatt
    10261 gatttttggg atttcgatga ctccaagcat atgttccata aaaaaggcat tactaggtaa
    10321 atcttttaat gcttttgcat caactgcttg gttgaaactg cctatccctg gtgatacgtt
    10381 ttgctctgcc aactgttggt ttttctttgt cattttttcc tgctgttttt ttatttcctc
    10441 tgaattctta ttcgatgctt gcttttgata atggatgatc atttgctggg caagaaaatc
    10501 attcagtgaa tcttctacaa aaggataaga aaagatcccg atcccaacga ctaaaatgat
    10561 tgccatgaag atgtccagta ttcttattct gcttttattc atcatgtcca cctattttct
    10621 aatatgtata tgaagaggtt gtgaaatcag cgccttgacc tgagtattag cgaagcaaac
    10681 ggcaaaatag ttcctcctat tttgccgttt gttgagcgaa taccaagaga ccgaccactt
    10741 gtcacaacct ttctccccat ttgaatcact atcgtttatt tatcgtatga tttttgtttc
    10801 caatagttta agcttctgtt tcttgatggc gttttttgaa atagatcact gctcctccaa
    10861 ctaaagcagt acctaccaag atgaatacga tgatccccat accaccagtt gaaggcaaga
    10921 ttcctttagg ttggttatcg atcgttgttt catcagaagt tgttggatca tcatctactg
    10981 taaattcagt gtacgcagct tcattcttag gtaatgcgta gccttctggt gcttttgttt
    11041 cttgaaggca gtatgcccct tgtttcagcc cttcgattga aaatttccct tctgaatcag
    11101 aaatcaattt cacttcttcc cagtcagaac ctgtgacttc atctgaccaa gcatatgggt
    11161 attctcctga ggcaaggttt gctatttttt tcattaattt aacagttgga tcttttgctc
    11221 ttagaataga aaattctgca tcggctaatt tttcttgcgt gttcacatct tgtttgatga
    11281 agtggaaatc accgtagttt tggacatttt ctacagtaat cgtgttatcc gtagttgtat
    11341 tttcccgatt gactacaccg tttggattcc caagattggc ttcatctgcg ccatggatga
    11401 taccggttgt ttgttgcaat tttccgtttt ttactataaa agtagtttct ttttgtggat
    11461 tggttaattg ataaccatct ggtgcttttg tttcaactaa agtataattg ccgtctatta
    11521 atccattgac actgatggat ccttcagtat cagttgtgaa tgtcaacgct gactcagcag
    11581 gatcatagag agcgacacca gtgttttcat caaaaccggt cacgaattga ttctctgaat
    11641 ttttgatctg gaattgcgcc cctgctaact tatctacccc tgaaaagttc ccagctttct
    11701 ggattttgat attgctttct ttgatgacat ttttagcata taggtgaatc gtgtctaaag
    11761 gtttgtcagg gtctagggta agcttaacac gatatttggc gacgtttcga caccgggctt
    11821 tggtgtttct acaaagacgt atacagcatc tttcccatca gatttacctg gaagagtcag
    11881 gttagctaat ccttcaccag aagtcgttac acttgcgaag ggtgtagcgt ctgctggaac
    11941 tgctgtgtct ttacttggat agtacgtagc agtgatgtca tatacgttga attcaacgcc
    12001 gttcaacggt tcgctattcg gaaatgtcaa ttccgttccg ttgtttattt gattttctgg
    12061 tgctgaagtg aatttcaatt tatgcaaatt aaccgtaaca ttttctggtg taggtgatac
    12121 ggctgcagaa acctctgcag ttcctaggcc gagaacgaaa ggaagcagca tgatagcagt
    12181 tcctaaaaac gtccatattt ttgttttgat gtttttcatt ttatttccct cctattgttt
    12241 cctttttgtt gttttttata aaaccaagct gtccctgttg aaaggactag caataaccct
    12301 aaaattgaga aacctccaag catctcttcg cttgtttttg gaaagcttcc tccatttttt
    12361 tcatttgatg gaccatttgt ctgcatctga actggcgttt ttccattatt cgtcggattt
    12421 gcttttggtt cgttgatgat ttccaatggt ataggatgaa gcggatcata actgcctttt
    12481 tcaacttcaa agagaatggt tgattgactc aatatatatc ctttaggcgg tttaatctcc
    12541 actagttcgt aacttccaaa agacaatcca tggacagaaa atgttccatc tttactggaa
    12601 gtaaaaatcg ttgctcttga atcatctttg gctacccaat gatttatccc gttttcttgc
    12661 gctaagtatt ggtcattttt tttgactaga aattgagcat ctgctagttt ttgtttcgtt
    12721 tgccgatcca ctttgacaaa ttgtttgcct cctgtttgga tggcaagatg tgtttctact
    12781 gtttgattgt cagtcgtcaa acgtacatcg ttatcaaatg tcgttttttc actgttttct
    12841 ttcaatgtca tttggtaacg gatcgttaat ttttttccag caaacgaact aagatctttt
    12901 ccagaatctg ataagacaaa accatgtttt attttttgtg tgtgaaatgt atgaatcttg
    12961 tctccaccaa tcaaaaaatc gatcgtttct ggttctagcc ataactgagg atcagctgta
    13021 tctttgatcg tataggttcc tatctcgttg atgttctcag gaattgttac ggtgatgaga
    13081 taagggactt tatccccatc tgcaaaatct gtctgcttat tctctactgt ttttgttaat
    13141 gtgagtgcag caggtatttt ttgttcgctc tttggataca agtggatggt cgtcaattta
    13201 tgattagatg aatctgtcac aggaagcaca acgacaaatg gtgctgattt ttcttttact
    13261 taatctgaag tctttatttc cgtaaatcgg taaacagcgt cacgaccttg ctcatctttg
    13321 tccgatgcac taaaagaagc aatgccctct tcctcgtcta ctgtttttgt cacattttct
    13381 gccagtattt tttcactatc acttttttgt gctagttttc tttgtgcgtc ttccacacta
    13441 cttccttctg accgcagctt gtaaaattca tccgagacat catatatagc gaaagtaacg
    13501 ccgttcaacc cttttactcc ttgaaagtta tccgggatat ttattccgtc gttttgactg
    13561 atacttggca tcttatcgct ttcgaacatt cgattatgca aagtaaatgt aataggtgat
    13621 tctctagctg acacagtcgg agaacctata aaaaagacca gtaggataaa aaagagaaca
    13681 gcaaatatat ccacacgtac attttttgtt tttttcatcg ctgttcacct gcttttttct
    13741 tacgatggat aaaataaaag aagccagaaa aaataaagat cagtcctaaa aagacaaagg
    13801 gcagtagacc tatcccacct gtcgaaggaa gcaagagagt cggattgatc gtcaacttag
    13861 cggtatctgt tgcgataatc gtataattat tgtttacact tccttcgcct aaactaatcg
    13921 ttactatttg tgatccaata tctgtccggc tgttaactgt tagggtataa tccaatgatt
    13981 cgcctaagac aacaccggta acgtggagat ttccaaccga aatcgagtga ctttgggcat
    14041 cataattaaa ggcagcgtca tcgacagtaa tcactgcgtt agctttatag atggtataag
    14101 ttcctgatgt gttggctata tcagtaaatg acaacttggg atttgctgct ttgactttgt
    14161 ctattccgct attcgttagt ttaattgtat agctgccagc attaaccata ttttttgctg
    14221 attgtgcatc tgctgtgtaa tccatactgc taagtgccac ttgtactgga gtaagcgaac
    14281 tagtgacacc ctctgctagc ttgacatgga ttgacgggac acctgctttt ccatcatatg
    14341 tcttactgcc actgagctct accgcaccgc tgatttgctt aatcgtatag acatcagatg
    14401 tctgcacgat agtaaataaa tagtttgttc tggcagctag tttagctagt ccgctttttg
    14461 tcaattgcca agacgtatag gttccaattt cagtaggcgc agttacttca actccctttt
    14521 cattcatcgc tttgaaatca gttggattcc atgtgatttg atcaatactg agatcattgt
    14581 ttaaaaggga tgttgattct gttttatcat taaaggcata tttaacgatc ggtacaacag
    14641 aaagaccatc atagtactta ctgccatttg cattgattga tactgttgca tttgtcttat
    14701 aatataggat gacttcagaa atctgttttc catttgcgtc ctttacgatt acatttccat
    14761 ttgtatctaa actagctaaa acactcattg ctgattggtt ctttgtttct atcgctgaaa
    14821 cagctgtaaa ggtatatccg ttgatagtag gcgtattagt cgtcaggtct tgtgcagcac
    14881 ttccgtaagt aagattcatt attttagaag gagcaatcat ctgtccaaaa tagtcttgat
    14941 acttaatagt gattggtgtt tgtttgcctg tataggtcac cgtatcgacg ttactttcta
    15001 gggctgtgaa agaaccgttt gtttgtgcaa aagaatagcc cgttactgct gttggagtga
    15061 taaccacttt gtcacccaca tggattccag ataatgtagt cccgtacgtt ccgatttttg
    15121 ctgctccacc attgactgta taagaaaaag ttcctgtccg attcgcattg acctgaaccg
    15181 ttgctgcttg ataggttgga aaatttgaaa catttccctt accatcaacg atctgcccat
    15241 ttttgatcga gtaccccgtg aatggaatat catttgctaa ctgtataccg attgaaaaag
    15301 tactcatacc tcctccggca ggaagccctc cccatgttga gggtgtaaaa gaataacttt
    15361 tatccgcatt gggtgtaact gaatcgatcg tatttccagc ggtagccacc cccatataga
    15421 tactttttgg gaatgtgata gtagtaccat ttactgtgga gccaataaac agattatgga
    15481 tatctggacg gttatcaata aaagcgattg ctttggttac gccttgtaac gcagttttat
    15541 ccgcactcat cgatccgtta accgcaacaa tgactccatc tttggcatat atcactgtat
    15601 atccatctgg tatggtatgt ccgttgaaat tcgcagtggc cgcaacagta ctaaatgtct
    15661 ggcttctacc tgtttctgaa taagctttct cccctgctgc ttttgctgct gtgatttctt
    15721 tttctgtcgg atcaatcagt tgagtcgagg atgtggttga ggttgtctcg ttgttgctag
    15781 actgattggt gctttctatg gactgtattt cagaataggg tggactattt gtctcctctg
    15841 tcgtttggtt agcaaaattt gacgtttctg tataaacgtt tacataaata cttgataagc
    15901 tccctaagaa agtaaaaatc aagatgatcg aaatcataat gggatgtaag tattttcttt
    15961 tagatacagc tttgagtcta gaaacaaaat gtttttgaca tatacgttgg attttatttt
    16021 gagttttctt aatcattttc atccctcctc tcaataaaaa a
//
LOCUS       DAHUVV010000058        15995 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.059, whole genome shotgun sequence.
ACCESSION   DAHUVV010000058 DAHUVV010000000
VERSION     DAHUVV010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.059"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            226..894
                     /locus_tag="NK767_001837"
     CDS             226..894
                     /locus_tag="NK767_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCH6694832.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="NK767_001838"
     CDS             complement(1094..1324)
                     /locus_tag="NK767_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694833.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="NK767_001839"
     CDS             complement(1451..2833)
                     /locus_tag="NK767_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCH6694834.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="NK767_001840"
     CDS             complement(2989..4176)
                     /locus_tag="NK767_001840"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCH6694835.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="NK767_001841"
     CDS             complement(4183..5214)
                     /locus_tag="NK767_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCH6694836.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="NK767_001842"
     CDS             complement(5230..6684)
                     /locus_tag="NK767_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6694837.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            6859..7764
                     /locus_tag="NK767_001843"
     CDS             6859..7764
                     /locus_tag="NK767_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCH6694838.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="NK767_001844"
     CDS             7855..8532
                     /locus_tag="NK767_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCH6694839.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="NK767_001845"
     CDS             8529..8699
                     /locus_tag="NK767_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694840.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="NK767_001846"
     CDS             8813..9709
                     /locus_tag="NK767_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCH6694841.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="NK767_001847"
     CDS             complement(9764..11134)
                     /locus_tag="NK767_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6694842.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="NK767_001848"
     CDS             11372..12118
                     /locus_tag="NK767_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCH6694843.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="NK767_001849"
     CDS             12115..12954
                     /locus_tag="NK767_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCH6694844.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="NK767_001850"
     CDS             12955..13905
                     /locus_tag="NK767_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCH6694845.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="NK767_001851"
     CDS             14046..14798
                     /locus_tag="NK767_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694846.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="NK767_001852"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="NK767_001852"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCH6694847.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5192 a         2997 c         3221 g         4585 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctacattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agttt
//
LOCUS       DAHUVV010000059        15849 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.060, whole genome shotgun sequence.
ACCESSION   DAHUVV010000059 DAHUVV010000000
VERSION     DAHUVV010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.060"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            286..1425
                     /locus_tag="NK767_001853"
     CDS             286..1425
                     /locus_tag="NK767_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCH6694848.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1492..2067)
                     /locus_tag="NK767_001854"
     CDS             complement(1492..2067)
                     /locus_tag="NK767_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCH6694849.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2072..3046)
                     /locus_tag="NK767_001855"
     CDS             complement(2072..3046)
                     /locus_tag="NK767_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCH6694850.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3070..3114)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3329..4297
                     /locus_tag="NK767_001856"
     CDS             3329..4297
                     /locus_tag="NK767_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCH6694851.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            4410..4688
                     /locus_tag="NK767_001857"
     CDS             4410..4688
                     /locus_tag="NK767_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCH6694852.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            4806..5042
                     /gene="secG"
                     /locus_tag="NK767_001858"
     CDS             4806..5042
                     /gene="secG"
                     /locus_tag="NK767_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCH6694853.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            5145..5900
                     /locus_tag="NK767_001859"
     CDS             5145..5900
                     /locus_tag="NK767_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCH6694854.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5903..8260
                     /gene="rnr"
                     /locus_tag="NK767_001860"
     CDS             5903..8260
                     /gene="rnr"
                     /locus_tag="NK767_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCH6694855.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8282..8746
                     /gene="smpB"
                     /locus_tag="NK767_001861"
     CDS             8282..8746
                     /gene="smpB"
                     /locus_tag="NK767_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCH6694856.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9048..9767
                     /gene="atpB"
                     /locus_tag="NK767_001862"
     CDS             9048..9767
                     /gene="atpB"
                     /locus_tag="NK767_001862"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCH6694857.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9820..10035
                     /gene="atpE"
                     /locus_tag="NK767_001863"
     CDS             9820..10035
                     /gene="atpE"
                     /locus_tag="NK767_001863"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HCH6694858.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            10126..10650
                     /gene="atpF"
                     /locus_tag="NK767_001864"
     CDS             10126..10650
                     /gene="atpF"
                     /locus_tag="NK767_001864"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCH6694859.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10637..11179
                     /locus_tag="NK767_001865"
     CDS             10637..11179
                     /locus_tag="NK767_001865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCH6694860.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            11202..12758
                     /locus_tag="NK767_001866"
     CDS             11202..12758
                     /locus_tag="NK767_001866"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCH6694861.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12772..13674
                     /locus_tag="NK767_001867"
     CDS             12772..13674
                     /locus_tag="NK767_001867"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCH6694862.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13697..15103
                     /gene="atpD"
                     /locus_tag="NK767_001868"
     CDS             13697..15103
                     /gene="atpD"
                     /locus_tag="NK767_001868"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCH6694863.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15117..15539
                     /locus_tag="NK767_001869"
     CDS             15117..15539
                     /locus_tag="NK767_001869"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCH6694864.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         5264 a         2840 c         3419 g         4326 t
ORIGIN      
        1 tgcaactagc ttctgtatat gatgtttaca aagaatggta caagaaaaga tcatccttga
       61 aaaaggaaag ctaacaataa ctagcatcat ataagagctt ttaaggatag aacagtagaa
      121 taggaatcta tgagcaagtc ctctcgtata aataaaatag gagggaagta gcagcatatt
      181 tttcataaag tggctctctt tcttcatttt tactttggtt gctcctattt gaaaattcga
      241 gcacactcaa atacaataaa aagaaatggc aagggagagg attagatgaa aaaagaagaa
      301 aaaatcgcga ttttacaaga gatcatccga attaaatcag tgaatggaaa cgaaggtgaa
      361 gtagcagcct atttgaataa gcttttggca aagcatgaca taacaggaga gattgtttct
      421 tacagagatg gtagagataa tttgattgct cgttaccaaa agggccaatc aggaaaagtg
      481 ttaggcctat ctgggcatat ggatgtcgtg gcggctggtg atgaatcgtc ttggacctat
      541 gcaccctttg cagcagaaat ccatgggaac cgtttgtacg gacgtggtgc tacagatatg
      601 aagagtggct tggcagcaat ggtgattgcc atgatcgaac tgaaagagtc aggaaaacca
      661 tttaacggaa cagtcaaatt attagccact gtcggagaag aagtgggtga actaggtggg
      721 gaacagctca cgaaagcagg ctatgtagat gacttagatg ccttgatcat cggagagcca
      781 actaattaca gtttgatgta tacgcacatg ggatcaatca actatacagt cacctcgcac
      841 ggaaaagaag cgcatagctc tatgccagac caaggttata atgcaatcaa tcacctaaat
      901 gaattcatta cgaaagcaaa tgctgaaatg aatcacctcg ctgaaacaat cgagaatcct
      961 gtcctaggaa aaacaatcca taatgttaca ttgatcagtg gcgggaatca agtcaacagc
     1021 atcccaagcc atgcgcagct ccaagggaac attcgatcga ttccagaata cccaaatgat
     1081 aaaatcattg cattgctgca atcaatcgtc aatgaattga atcaagagac agactatcat
     1141 ttggaattga tgatcgatta caacaaaata cctgtcaaag ctgatccaga ttctcctttg
     1201 attcattgca ttcagcaaca attcagccag ccattacctt tagttggagc ggcagcaaca
     1261 acggatgcag ccgaatttac gaaagctaac cattccttcg actttgtcgt ctttgggcct
     1321 ggtgtcgtta ctttgcctca tcaagtagat gagtatgttg aaatcgataa ttatttggat
     1381 atgattgaaa aatatcaagg aattatttta tcctacttag cgtaagattt ttttaataaa
     1441 tgaaaaaaca gagccagatt agcattattt tgctaatcca gctctgttta ttcagcctat
     1501 ttttcttttt gaatatatca agcgttgtgg caacatagtg atgactaatg aaacaacgac
     1561 gatagacaac aaacgatcaa ggtagtctgt acctgcctgt accaagatca cacttgttgt
     1621 ctgacttaaa cctaacccat gtaatagctg aacgatcata ctggatcctg aggaggtgat
     1681 cccgccaaat aactgaacgg taatgatcga agaaacgatc gttccaggta ccgacaaaat
     1741 caaagcgagt aataacagtt tcaagctgtt tttatctttg ccgagatatc ttggcaaaat
     1801 caatccagca agcaagcctg tgagaatttg gattggcgaa taatacagag aaaaaatatc
     1861 tgtagtcact ccacttaaaa taccgctgga caatcctgtc agcattccag gtacaggccc
     1921 aagcaatgca ccagcaaaaa tcgttccgat agaatcgaga tagattggca aacgaagcca
     1981 taaagcaatc gttcctccaa tataattgat cccaatacac aaagccatca ctgtaagtac
     2041 gcgagttgcc attttgtttt tattcatttt gttagcccat ctttaacctt tccaaatctt
     2101 tcataatcgt ttcttcttgt gcatttaaaa gaacggttaa aaactttcgg aaaaaaagac
     2161 gagtatctac ttttgtcata attttactgt tagcaggttt ctgccaaaat tcataacgat
     2221 caaccaatgt ttgtccacga cttattcctt gagtttccac agcagtgaag cttttgaaac
     2281 cttcacagat ggccccctcg ataaaataag caacagctaa cggatcattg atgacacatc
     2341 ctaaaattct ttcttgtttc cagtggaagt caaaataaaa acgagtgatc gcttttagat
     2401 actctccctc ttcaggattc atttgacagc agtattcgag gatagtcggt gtcaagacga
     2461 tttctcttgt cacatctaac ccaaccattt ctatcgtttg tttgagattc tcgaatacat
     2521 aagaagctgc ttcaggatca caccagtaat tgtattcagc aactggcgaa cagttaccat
     2581 ggcttttgta agttccaccc atcgagacaa aacgatccat atgtttccca atatttgggt
     2641 tgactttcaa agcaaaagcg atattggtca aaggacccaa agcaatgact gatgtatttg
     2701 tcttttcttt gaaataatca gccaaaaaat caacggcatg gagaggttct gcctgttttc
     2761 tcaagatcat cgggaaattt gtttccccta aaccatccat accatgagtg tcttgtgcac
     2821 tgatgaaagg ttgcttcaaa ggtttttcca ttccttgata aacaggaatg tctaaacgat
     2881 cacagcgttc caggcatttc agcacattct gcgtacctat atctgcaggg acattcccac
     2941 aaactacggt aatagcaact acttcgatat caggggattt gacagctaat gataaagcta
     3001 aagtgtcgtc gatccctgga tcgcaatcaa taattacttt tcgcattatt tccaatctcc
     3061 tttctttaga gatacttagt tttttattct gggaagtttc cgaaccagtc caagacgagc
     3121 gtcttgatta taatgcacga aattagtata ctggctttta ttgcgaatgt aaatgctctc
     3181 ttacaagtaa atctattgag gagaacttta agtaaatgat acaatgaaaa agaaatggaa
     3241 tttacgtgtg ccgaaaataa agaatcctat ggatcggtac attggataat agttagatta
     3301 tagtttggca gaaagcggcg ggtgtacaat ggttattttt tatttcattc ttattctgat
     3361 aattataggg atcagttacc attttctttc ttatttttat gcaaagaaat tagtaagagc
     3421 gggagtacag agaggatatg actggtatga agacactggt catcgtttga tggaccctgc
     3481 tgcagtcacg tcagtagaaa aaaaacgaca aaaattagaa gaagagtgtg aaagcttttg
     3541 ggagcaggga acacccaaaa caatcaaaag ttatgacggg ttgaaactag ctggtcagat
     3601 gttcatccat ccaaataagc agaataaatg ggtcatttgc gtacatgatt accgaagcac
     3661 agggaaaaga gacatgtctc atataggtaa aagatatgca gagaaaggat tcaacgttct
     3721 tgtacctgat ttacgtgcgc acggagaaag tgaaggagaa atcatcggaa tgggatggct
     3781 agatcggcta gatttaattg cctggatcca actcatctta gatgaacagc cgaatgcgtc
     3841 cattatttta cacggcggat caatgggagc ttctacgatc atgatggcaa gtggtgaaaa
     3901 acttccatca gcggtcaaag gatttatttt agacagcgga tatgtttctg tttatgcaga
     3961 attccgatat atgttaagta aaatcactgt ctttcctaaa aaaatggtca tgagatatgc
     4021 gaatcattat gcccagaaat acgccggtta ctccttaaag caagcatcag ctacgagaca
     4081 gcttggcagc aaccatctgc ctttattggt cattcatggt gaaagagatc attttgtccc
     4141 tacagaagca gcgtacacga ttcaaaatgc tacggcaggg gataaagcac tgctgcttgt
     4201 tccagaagca gaacacttgg aagcatcgat gaaagatccg aaaacctatt ggacagtgat
     4261 tttttcattt attgaacaaa gaataacgat gaattaattt tctcttcttt tttgtaagta
     4321 attcgaggat aaataattgt ttatagatca ggagactgct aaaataaggt aggaataaaa
     4381 aaatatcagt tatttgagga gtgcggcata tggaaatcaa agaagaaaaa aatcgtcttg
     4441 ctttattcaa tgatgaacaa caagagatcg gagaaatgac atggtcagat gcgggcccag
     4501 acatcatgat cattgaccat acatttgtag atccagcata tcgtggtcaa aagttagctg
     4561 aaaagctagt gtatactgga gtagaattag caagacgaga cgggaaaaag atcattcctt
     4621 tatgccctta tgcaaaaaaa gagtttgaaa agaaaccaga atatcaagac gtcttacgtc
     4681 agtcataaga ataacagctg aaagttttga actttcagct gttattccat ttatatagag
     4741 ttgtcacgga cttttatcta tgataaagtt aaagagacga tatcttttgg gaaggaggct
     4801 gcgatatgta taatttaatt ttgggaatcg tgatcgtttt atcgattgtt atggtcattg
     4861 cgattatgat gcagccaagc aagcaaaata gtgcagcgag tgcatttacc ggcggtgcgg
     4921 atcaactatt cggcaaacaa aaagctcgcg gctttgaagc tgtcatgcag cgatcaactg
     4981 cagttatggg agctatctgg atgatcttat tatttgtcct agcatattta tcttcgaagt
     5041 agttgaataa gcctctcgca aaacggcggg cggctttttt tattgcaaag agaacgctta
     5101 agaagaagtt atgaaagaaa ctatggtaaa atgaggcgaa agaggtgaga cgagtgagac
     5161 aattaccaaa acccctttat gcaaaacatg gaaagcgagc ggttttgctg cttcacgctt
     5221 actcaggaag tccaaacgat gttcgaatgc tcgcacgatt tttggaaaaa gcagattata
     5281 cagtttatgc gccgcttttt aaaggacacg gaacaatgga gccatatgat attttgcaag
     5341 aaaaagcaga aagctggtgg gcagacacta aaaaagccat ccatttttta cagtctgagc
     5401 agttttctga tattgccgta ttaggattat caatgggcgg catctttgct gtacgagcac
     5461 tagaagaaga atcagtgatt ggcggcggtt ttttctgttc gcctttgtct cctgtaaaaa
     5521 caaacgtgcc tgaaaacttt gaaaaatacg tacgtcaagt gctgaagaca gcaggtaaat
     5581 cagagaaaga aatcaatgaa aaagcagttg cttaccgctc gttagctgaa caacaattaa
     5641 tggatataca agatcaggca gcaatcgtag aaagtaggtt gtcagatatc caacagccga
     5701 tatttttagc tcaagctggg aaagatgaaa tgatcgaccc taatggcgta tttgaaactg
     5761 cacgcaagtt gagccgtcag cgagtgacac ttcgatggta tcctgaaagt ggtcatgtga
     5821 ttacagttgg tacagcaaga cgcgaattag aaaaagacgt cttggaattt ttagaaaaat
     5881 taccttggaa tgaggaataa atatgacaaa agaaacgata aaagagaaaa ttctcttttt
     5941 catggaaagc agtcgcaaac gcagtttttc tatggaagaa atcgctgaag gtttagggtt
     6001 ccaaaaaagc gatgatttta aattactagt acaaacgatt gctcaaatgg aaagagaaca
     6061 gagtatagtt ttcaataaaa aaggaaaagt gaaacttcct ttaaaaccaa tcttgatcga
     6121 aggaacattt cgagcaaatg aacgaggatt tggatttgta acgattgatc ccgaagaaga
     6181 tgacatctat atcccaaaag aagcgactgg ttttgcaatg gatggagaca ccgtagcaat
     6241 cgatatcatg aagcgagcaa atacagatat ggatcgcgga gcagagggga aagtagtcga
     6301 gattcgagac cgggcgacta ctcagttagc aggggaattc gttgcttatt cagaagaaga
     6361 aatacaagaa acggatcttt acggcgttgt tatcccgaaa gatagaaagc tcaaccaatt
     6421 taaagtatat gcagcagcag agggagttcg tccagttgac ggaagtattg tgctgatcga
     6481 actgacccat taccctgaaa aagggtatgc gactagttta gaagggataa tcaagcaagt
     6541 gattggtcat aagaatgacc ctggcatgga tatcctctca attgtcgtgg ctaatggtat
     6601 ccctacgaaa tttcctgatg atgtattgtc tgaagcagat gaagttccag atcaaatcag
     6661 cgaaaaggat ataactggac gtagagattt aagaaatcag ctcattgtta cgattgatgg
     6721 agaagatgca aaagatctag atgatgcagt tacggttaaa aaattagata acggtaatta
     6781 ttttcttgga gtacacattg cagatgtttc ttactatgtg acacaaggca gtcaattgga
     6841 cagagaagca tatgaaagag ggacaagtgt atatttgact gatcgagtga ttccgatgat
     6901 cccgcaacgt ttatcgaatg gtatctgttc actgaatccc catgttccaa ggctgaccat
     6961 gagttgcgaa atggagattg atccaaatgg acaagtagtc tctcatgata ttttcccaag
     7021 tgtgatccaa acaacagaac ggatgaccta cacagctgtc aatcaaatct tagaagatca
     7081 agacgaagaa acgatgcaac gctatgctca gctcgtacct atgttccaag aaatgggtga
     7141 attgcatcga atccttgaaa cgatgcgaat caaacgtggc gcgatttctt ttgaggatcg
     7201 cgaagcaaaa atccttgttg atgctgaagg acacccaaat gatatccagc taagaagcag
     7261 aggaattggt gaacgcttga tcgaatcttt catgcttgcg gccaatgaaa cggtagctga
     7321 acattataat aaattaaatc tgccgtttat ttaccgaatc catgaacagc caaaagaaga
     7381 aaaaatgcag cgcttctttg attttgcttc agctttgggc attctggtcc gaggaacgaa
     7441 aggaacgatc acaccaaaag atttacagca tgtgatcgaa ggagtagaag acaaaccaga
     7501 agctgcagtt atcaatacta tgttgctaag aagcatgcaa caagcacgct attcagaaga
     7561 caattttggg cattatggct tagcggcaga ttactacaca cactttacat ctcctattcg
     7621 acgctatccc gatctaatcg tccatcgtct gatccgttct tacagtgaag ataaaagtga
     7681 aagcaatcaa agttcgtggg ctacggaact tcctgaaatt gcagatcata gttctaagat
     7741 ggaacgtcgt gcagtagaaa cagaacgtga agtagattcc atgaagaaag cagaattcat
     7801 gatggacaaa gtgggggaag aattcgatgg gatcatcagc tctgttgtga aattcggcct
     7861 gtttatcgaa ctgccgaata cagtagaggg attgatccat atcaatgaat tgaaacagga
     7921 ctacttccat tttgttgaaa atcatttagc tcttgtaggt gaaagaactg gtctgacttt
     7981 taaaatcgga cagaaagtgc gagtaaaagt agtcaaagct gatccagatg aacgagcagt
     8041 agattttgaa cttgtttccg cagaagaggt tacagcgttg gaacggccaa aaacacgaaa
     8101 tggaaaatca ggaaaacgcg aaggaaaaca agcaggaaaa cgcccagaaa gaaatggcaa
     8161 gaagaacaaa cgatatagcg atagtaagca aacgttcggt aagaaaaaag ggaagaagcc
     8221 attttacaaa ggtgttaaaa agaagaaaaa gaaaaaataa aatgagttgg aggaaacagc
     8281 tatgccaaaa ggcgagggaa aattaattgc acaaaacaag aaagctcgcc atgattattc
     8341 gatcatcgac acgatggaag cagggatggt cttgcaagga accgagatca agtcgatacg
     8401 aaacagccgg atcaatctaa aagatggatt tattcgcgtc cgcaacggag aagctttctt
     8461 gcataatgtt catatcagtc cttatgaaca aggaaatatt tttaatcatg atccgttgcg
     8521 cacgagaaag ttattattac acaaaaaaca aatcatccgg cttgaaaatg aattgaaaaa
     8581 tactggaatc actgttgttc ctttaaaagt ctatattcgt aacggctatg ccaaggtatt
     8641 gattggtctg gcgaaaggga aaaaatctta tgataaacgg gaagatttga aacgaaaaga
     8701 tatcgatcga caaattgatc gaacattaaa aaatttctct agataatttc attatccttt
     8761 ttcgcatatt cggtgctttt gcttttactt aatgataaaa tttgttagca tttaatctga
     8821 taaaacatga ttatcatagg ttttttctta gatcaggagc accgtgtata aagaaaaatt
     8881 cgcttcgttt ttgactgaaa taaaaaagtg tctaattttc atgaaaatgt tttgaatttt
     8941 ctgtttcatg gtggtatgat accgtgtggt tagaaaccga ttggaaaaaa ccagatacgc
     9001 acatgaattg attttatgtg aatttcgggg aaaagaggtg aaatggattg gatgaacgct
     9061 cgctaacgtt ccacattgga ccaatttggt ttgatggtac agtttgtata atggtgttac
     9121 tgacctgttt gattgttttc ttcttagctt acttttttac aagaaatctt aagttgaaac
     9181 caacaggcaa acaaaatgcg atagaatggg tcattgattt tagtcgagga atcgtaacag
     9241 ataatctgcc tagaaaagaa ttgagcaatt tccatttgtt ggcatttaca ttgtttttat
     9301 ttgtttttgt tgcaaacaac atcggtttag taacaaaaat cgttttacct aacgaaacaa
     9361 cactttggaa aagcccgaca gcagacccgt ttgttacttt aactttggca tttataatga
     9421 tcttacttac tcacttattt ggggtgaaaa agttaggctt caaaggatat ttcgtaaatt
     9481 cgttcttgaa accatatagt tttatgtttc cgatgaaact gattgaagaa tttacaaact
     9541 tattaacact tgctttacgt ctttatggga atatttatgc gggggaagtt ttgctaacct
     9601 tgattgcaaa aatggtaata agcttaggct ggttctcatt gccgttagca attccattag
     9661 agatggtctg gatcgcattc tccttgttta tcggaagcat ccaagcattt gtcttcgtta
     9721 ctttatctat ggtttatatg agccataaga ttgaagtgga agaataatat tcgttttaaa
     9781 aacaaactac tatttatcta atttaggagg aaaacaatta tgaattatat cgcagcagca
     9841 atcgcaatca tgggagcagc aatcggagcc ggttatggta acggtcaagt tatctctaaa
     9901 acaatcgaat caatggctcg ccaacctgaa atgtctggtc aattccgtac aacaatgttt
     9961 atcggggtag ctctagtcga agctgttcct atcttagggg tagttgtcgc cttgattcta
    10021 gtttttggtg tttaattgaa tcagtattaa ggcagaaaca atttggtttg ttactgcttt
    10081 tttacatgaa aactagaagt acttcaaaga aaggaaggct cgcctatgct gaatcaattg
    10141 gcaattgcag aagttggcaa tgcgatgtta ggaaatgtca tcgttgtcag cggctcattt
    10201 ttgatactac tggctctctt gaagcatttt gcttggggac caatcagcga tattttgaaa
    10261 aaacgtgaag acaagatcgc caatgatttg gattctgcag aacaatctcg catcaactca
    10321 gcgaaaatgg aacaagaacg cgaacaacaa ttgttagcct ctcgttctga tgcagctgat
    10381 atcatcaaaa atgcgaaaga aagtggagaa ttaagccgcc aaaatatttt gaaggatgct
    10441 caagaagaag cagctcgtct aaaaagtaaa gcccaagctg atatcactgt agaacgtgat
    10501 tcagcgctga actctgtaaa agacgatgtt gcagaactat ctcttcaaat cgcggaaaaa
    10561 atcttgaaca aagaattatc tccagaaatg cacgaatcat taatcaatca atatatcgaa
    10621 ggtctaggtt cttcaaatga aactagataa atatacagta ggaagacgtt acgggaaagc
    10681 tttatttgaa ctggcgatcg attcaaataa agcagaagaa atttatcaag atctcgtaag
    10741 cttacgtcaa atctatgaac aagtacctgg attaggtaat atgttaagtg atgttcgtct
    10801 agaacctcat gaaaaacgag aaatcatgga caagcttgtt agtggctatg atggaatcgt
    10861 taaaaacttt ttagaagttg tttatgcgta taaccgaatg gatgatcttt tgttcatgat
    10921 cgaagaatat gaacgtcgat atgatgaaca tcaaggtttg ctgttgggaa gtgtcacaac
    10981 agcagttcca ctttcagacg aacaacgaat acaattagaa aagaatgttg caaaaacaat
    11041 gaactatcaa tcagtcgaat taaaacaaat cgttgattct tctattattg gtggcgcgat
    11101 tgttgaagct aaccatcgag taatcgacgg aagcattcgc acgcaattag aaaatatgcg
    11161 taatcaatta aatagatagt acaaagaggt aggtgaatcg aatggccatc aaagcagaag
    11221 aaattagtgc cttgattaaa gaacaaattg aaaattatca aaacgtgctt tcagtcgaag
    11281 aaatcggtac cgtaacttat gtaggtgacg ggatcgctcg tgcacatggt ttagaaaacg
    11341 cgatgagtgg tgagttgctt gaattttcaa atggctcata cggaatggcg caaaacttag
    11401 aatcaaatga tgtaggtatc attatccttg gcgatttcga gtccattcgt gaaggagata
    11461 aagtaaaacg aacaggaaag atcatggaag ttccagttgg agaggccttg attggacggg
    11521 tagttaatcc gctaggtcag ccaattgacg gactaggtga aatcgttact gacaaagctc
    11581 gtccagtaga agcaatggca ccaggcgtta tgcaacgtaa atctgttaac gaaccaatgc
    11641 aaacaggtct aaaagcaatc gacgctcttg taccaatcgg acgcggccaa cgtgaattag
    11701 ttatcggtga ccgtaaaaca gggaaaactt ccattgcgat cgatacgatc atcaaccaaa
    11761 aaggtcaaga tatgatctgt atctatgtag caatcggaca aaaagattct acagttcgta
    11821 cacaagttga aacattgaaa aaatatggcg caatggatta cacaatcgtt gtgaatgccg
    11881 gtgcgtctca accagcgccg ttgctttata tcgcaccata tgctggtact gcaatgggtg
    11941 aagagttcat gtacaacggg aaacatgtct tgatcatttt tgatgatctt tcaaaacaag
    12001 ctgttgctta tcgtgaactt tccttgctgc ttcgccgtcc accaggtcgt gaagcttatc
    12061 caggggatgt tttctactta cactcacgct tattggaacg tgcagcaaaa ttaagtgatg
    12121 atttaggtgg cggttcaatg actgccttac catttgttga aacacaagct ggagatatct
    12181 ctgcttatat cccaacaaac gttatttcga tcactgatgg acaaatcttc ttagaaagcg
    12241 acttgttcta cgcaggtact cgtccagcgg ttgatgccgg actatctgta tctcgtgtag
    12301 ggggttctgc gcaaatcaaa gcaatgaaaa aagttgctgg gactttacgt ctagacttag
    12361 caagttaccg tgaattagaa gcatttacac aattcggttc tgacttggat gcagcaacac
    12421 aagcaaaact aaatcgtgga cgccgtacag tagaaatctt gaaacaaaaa ttgcatgcac
    12481 cgctagctgt tgaaaaacaa gtagtgattt tgtatgcttt gacacatgga ttcttagata
    12541 gtatccctgt agacagtatt ttagacttcg aacatgaatt attcgaatat ctggatacca
    12601 atcattcaga tatctttgaa acaatccgta caacaaaaga tcttccagaa gaagaagcgt
    12661 tgaacagtgc gattcaggaa tataaagaca tgttcttagc tacaaaagga aataattcct
    12721 ctacagaaga taaattgaaa tccattcaaa atgcatagtg aggtgagatg aatgggtgct
    12781 tcattaaatg aaatcaagac acgtatcgct tcaacgaaaa aaacgagcca aatcacgcgg
    12841 gcaatgcaga tggtgtctgc ttctaagttg acaaaatctg aagcttcttc acagaagttt
    12901 caggtttatg caacaaaagt aagagaaatc gttacgcatt tgactgctac tcagttaaat
    12961 gatattgcct cagaaaatcc tagaggggat atcaattaca acagcatgtt gctcagccgt
    13021 cctgttaaga aaacgggtta tatcgtcatc actgcagatg gcggtttagt cggtggatat
    13081 aacagctcga tattgaaaca aacgatgtct attttagaag aagaccacca atcacccgat
    13141 gattacgtga tgatcgcaat tggcggaact ggtgcagact tctttaaagc acgcggtatc
    13201 aatctagcat atgagctgcg caatctatct gatcagccaa gctttgatga agtccgcaaa
    13261 atcgtaaata tggcaacaac tatgtaccaa aacgaagtat tcgatgaatt atacgtatgt
    13321 tataaccatc atatcaattc gctaactagt cagtttcgtg tagaaaaaat gctgccaatc
    13381 tcagacttag atccagaaga agcaactact ttcgaacaag aatatatttt tgaaccttca
    13441 aaagaagaaa tcttgtctca gttattgcct cagtatgctg aaagtttgat ctacggtgcg
    13501 atcgttgacg ccaaaacagc agaacatgct gctggtatga cagcaatgaa aacagcaact
    13561 gataatgctg ctaatatcat tgatgagctg acggtttctt acaaccgtgc aagacaaggg
    13621 gctattaccc aagaaattac cgaaattgtt gccggagctt cggcactaga atagtattga
    13681 gattggagga aaaaagatga gttcaggcaa gattgttcaa gtaatcggtc ccgttgtcga
    13741 cgtggaattt tctttagacc aatccttacc agatatcaac aacgcattag ttgtttataa
    13801 aaatgacgaa aataaatcaa aagttgttct tgaagcagcg ttagaattag gggacggagt
    13861 gatccggacc attgcgatgg aatcaacaga tggtttacaa agaggaatgg aagtcattga
    13921 tacaggcaaa gcaatctctg ttcctgtagg taaagaaaca ttaggtcgcg tattcaacgt
    13981 actaggagat acgatcgatt tagaaacacc tttcccagaa gatgcggaaa gaagcgaaat
    14041 tcataaaaaa gcaccagcct ttgacgaatt aagtacaagt acagaaattt tggaaacagg
    14101 gatcaaagtt atcgatttgc ttgccccata tttaaaaggt gggaaagtcg gactattcgg
    14161 tggtgccggt gttggtaaaa ccgtactgat ccaagaactg atccataata tcgcccaaga
    14221 acatggtggt atttctgtat ttaccggtgt aggtgaacgt actcgtgaag gtaatgactt
    14281 gtattatgaa atgaaagatt caggagttat cgaaaaaaca gccatggtgt tcggacaaat
    14341 gaacgaacca ccaggtgcac gtatgcgtgt tgctttgact ggtttgacga ttgcggaata
    14401 tttccgtgat gtagaaggtc aagatgtact gttgtttatc gacaacattt tccgtttcac
    14461 tcaagctgga tctgaagtat cagccttgtt aggacggatg ccttctgcgg ttggttatca
    14521 accaacattg gcaacagaaa tgggtcaatt gcaagaacgt atcacatcta cgaaaaaagg
    14581 ttcgatcaca tcgatccaag caatctatgt gccagccgat gactacactg acccggcgcc
    14641 ggcaacagca ttcgcccatt tggatgcgac aaccaacttg gaacgtaaat taacagaaca
    14701 agggatttat ccagcggtag atccattggc ttcttcttca agtgctttag cacctgaaat
    14761 cgtgggagaa gaacattata aagtagcaac agaagtccaa cacgtattgc aacgatatcg
    14821 tgaattacaa gatatcattg ctatcttagg tatggatgaa ttatccgatc aagaaaaagt
    14881 gttagtttct cgtgcccgca gagtccaatt cttcttgtca caaaacttta acgtggctga
    14941 acaatttact ggtcttccag gttcatatgt ccctgtagaa gaaacagtga aaggattccg
    15001 cgaaatcttg gaaggcaaat acgatgatct tcctgaagaa gctttccgaa gtgttggccg
    15061 aatcgaagac gtcgtagaaa aagcgaaaac actaggctac tagaaagggg tgccccatgg
    15121 atcaatattt aacagtaaat gtggtaactc ctgccggttt ggtctatgat caccatgcag
    15181 ctatcgtcgt tgcacgtacg acagctggtg agcttgggat tttgcctaaa catgcaccaa
    15241 tcatcgttcc attgacgatt gatgaagttc gtgtaaaacg aacagattca gatacccacg
    15301 tcgattggat cgctgttaat ggcggaatca tggaagttcg tgataatgtt gtttcaatcg
    15361 ttgctgatag tgcggaacgt gaacgtgata tcgatgtatc tcgtgcggaa cgtgcaaaac
    15421 aacgcgcaga acgccaaatc gaagaagcaa aagcaaaaca agatacgaac gaattgaaac
    15481 gggcaactgt tgcgttgcac cgagcaatca atcgtatcaa agtatctaag cactcataaa
    15541 atgagatgcg gatgtttatc cgtgtttaag aacagtaaaa agaggttatg acagaagtgg
    15601 tcagcttcaa gacataagaa ggaatttacg aaaattgctt ctcaaatttt tgtgaaatct
    15661 cagcttattt ttcgaagaag ctacttctgg aacaccgtat atcggaggtt gtgaaatcag
    15721 cgttttgtcc tgagtattag agaaacaaat ggaaaatagt tcctcatatt ttgccgtttt
    15781 ttgagctaat accgagggtc agatgattga acaccgttta tttggaataa gggactgcga
    15841 caagacttt
//
LOCUS       DAHUVV010000060        15811 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.061, whole genome shotgun sequence.
ACCESSION   DAHUVV010000060 DAHUVV010000000
VERSION     DAHUVV010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.061"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(59..187)
                     /locus_tag="NK767_001870"
     CDS             complement(59..187)
                     /locus_tag="NK767_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCH6694865.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="NK767_001871"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="NK767_001871"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCH6694866.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="NK767_001872"
     CDS             complement(1674..2729)
                     /locus_tag="NK767_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCH6694867.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="NK767_001873"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="NK767_001873"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCH6694868.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="NK767_001874"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="NK767_001874"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCH6694869.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="NK767_001875"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="NK767_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCH6694870.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="NK767_001876"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="NK767_001876"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCH6694871.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="NK767_001877"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="NK767_001877"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCH6694872.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="NK767_001878"
     CDS             10491..12050
                     /locus_tag="NK767_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HCH6694873.1"
                     /translation="MKKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="NK767_001879"
     CDS             complement(12161..14092)
                     /locus_tag="NK767_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCH6694874.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="NK767_001880"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="NK767_001880"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCH6694875.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="NK767_001881"
     CDS             complement(15021..15773)
                     /locus_tag="NK767_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCH6694876.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
BASE COUNT         4065 a         3470 c         2897 g         5379 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atgaaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt t
//
LOCUS       DAHUVV010000061        15801 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.062, whole genome shotgun sequence.
ACCESSION   DAHUVV010000061 DAHUVV010000000
VERSION     DAHUVV010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.062"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(182..544)
                     /locus_tag="NK767_001882"
     CDS             complement(182..544)
                     /locus_tag="NK767_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694877.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(618..998)
                     /locus_tag="NK767_001883"
     CDS             complement(618..998)
                     /locus_tag="NK767_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCH6694878.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1062..2255
                     /locus_tag="NK767_001884"
     CDS             1062..2255
                     /locus_tag="NK767_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCH6694879.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2331..2915)
                     /locus_tag="NK767_001885"
     CDS             complement(2331..2915)
                     /locus_tag="NK767_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCH6694880.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3116..4114
                     /locus_tag="NK767_001886"
     CDS             3116..4114
                     /locus_tag="NK767_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCH6694881.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4226..4735
                     /locus_tag="NK767_001887"
     CDS             4226..4735
                     /locus_tag="NK767_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCH6694882.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4765..5424)
                     /locus_tag="NK767_001888"
     CDS             complement(4765..5424)
                     /locus_tag="NK767_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HCH6694883.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5426..6190)
                     /locus_tag="NK767_001889"
     CDS             complement(5426..6190)
                     /locus_tag="NK767_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCH6694884.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6198..6875)
                     /locus_tag="NK767_001890"
     CDS             complement(6198..6875)
                     /locus_tag="NK767_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCH6694885.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6912..8297)
                     /locus_tag="NK767_001891"
     CDS             complement(6912..8297)
                     /locus_tag="NK767_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCH6694886.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8507..8591)
                     /gene="ffs"
                     /locus_tag="NK767_001892"
     ncRNA           complement(8507..8591)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="NK767_001892"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8614..9129)
                     /gene="tadA"
                     /locus_tag="NK767_001893"
     CDS             complement(8614..9129)
                     /gene="tadA"
                     /locus_tag="NK767_001893"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCH6694887.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9236..10093
                     /locus_tag="NK767_001894"
     CDS             9236..10093
                     /locus_tag="NK767_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6694888.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10174..10509)
                     /locus_tag="NK767_001895"
     CDS             complement(10174..10509)
                     /locus_tag="NK767_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694889.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10576..11946)
                     /locus_tag="NK767_001896"
     CDS             complement(10576..11946)
                     /locus_tag="NK767_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HCH6694890.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12024..12560)
                     /locus_tag="NK767_001897"
     CDS             complement(12024..12560)
                     /locus_tag="NK767_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCH6694891.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12869..14350
                     /locus_tag="NK767_001898"
     CDS             12869..14350
                     /locus_tag="NK767_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6694892.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            14515..>15801
                     /locus_tag="NK767_001899"
     CDS             14515..>15801
                     /locus_tag="NK767_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HCH6694893.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIK"
BASE COUNT         4907 a         3133 c         2613 g         5148 t
ORIGIN      
        1 tgtgaaatga tttttcgatc aattattatt attcgttata caaccgtttt caacatattt
       61 tgaaaataaa atgcactaaa aaactatctt tagcagaaca tttcattgta ttttttggac
      121 caaacaataa aaaaagaagt ttgaaaaaaa ttcaaacttc tttgttcgct taatctgtat
      181 tttagaagaa aggcactccc tctacttcgt aagttccaag aacagagcgc atttgacctg
      241 gttcttccca aatatatcct tcgcctaatt cttctctttg attcttttgt tcagtttcaa
      301 agacaaacaa cggaacattt tcctctttaa tatgtgtatt tgtcaattcc atcaaactaa
      361 tcttgctaac atcgatatga actatttctt ttaagaaatt aagtatattc gctaaaccag
      421 tctttccttc agaaagttcc gctaacgcaa attccaactt gtcatcaatt gaatggacca
      481 aaaatttttt agtaccatcc tccaaatgca acataattgt accagcaact cttttgcttg
      541 ccaaaatttc cacctcgatc aacttgttct atggcttaca taaaaattct agcacatttt
      601 cagaaaaaat acacttttta tttcttattt tctttccgtt ctagttcaga taatgcttct
      661 tctatccaaa taggtaactg ctccgcaata gtggtaaatc cgatcttacg atttttattc
      721 gtgaaatacc tttttctgcg atatggaaca gctggatttg ttttttctaa cgttcttaaa
      781 gataaactga tttttttgga atattcatca atgtcgatga tctgaactgt tactggctgt
      841 ccgactttta acaatgaatg gatatttttc gtataaccgg attgtacttc tgaaacatga
      901 ataagtcctt gcgtctcgtt atcaagagaa acgaatgcgc cataaggctg tatccctgtc
      961 acttttcctt caattatttg tccaatttta taagtcatct acttccctct cttcgcattt
     1021 atcattatac tgtaaatagc tcaaaaaaag aagcgacgaa aatgactgga ggtcttaaga
     1081 tgatcgaatt tgataaacag atttcaagaa aagatacaga aagtgtaaaa tgggacgcaa
     1141 ttgctgagac atatcaaatg gatgacttac ttcctttgtg ggtagcagat atggacttct
     1201 tgtctcccgg tggggtagct aaagcgttta gtgaatacat caaacatgga atctttggtt
     1261 atgccactct gtctgataaa ctatatcagg ctatcattca ttgggaacgc caacaccatg
     1321 ctgtggagtt gacgaaagaa aatatcgttt ttacaagtgg tgtgttaacc agtttagctg
     1381 cagcagtaca aacttttaca aaaccaggcg attctatttt gattcatgac ccagtttatc
     1441 ctccattttc ctctattata gaaacaaatc aacgtcacat cgttcgaagc tctttgctag
     1501 aaaaaaataa tcatttcatc atggacttgt ctgacatgga gaaaaaaatc gaagaaaaca
     1561 atgtaaaagc aatgatttta tgcaatcctc acaatcctgg agggcgtgtt tggagcaaag
     1621 aagaattgaa acagttaagc gaattgtgtt taaaacatca agtgattctg tttagtgacg
     1681 agatccatca agatttaact ttattcgatc atacatttac ttccatgcta acgattgatc
     1741 ctaatttaga tgatctacta gttgctttta cttccgcaac caagactttc aatctagctg
     1801 caatcaaaaa ttcaatggta tttatcaaaa atccagagat gaaagctgat tttgaaaaac
     1861 atttagtgat gaaccaacag cacgaaatca acacttttgg actgattgga acccaagcgg
     1921 cttatgagac gggtggagaa tggttaagcc aactgattcc ttatctagaa aaaaatgttc
     1981 aaacagttaa tcagtttttt gaacagtatc ttccgaaagt gagaataatg aaccctgaag
     2041 gaacttactt aatgtggtta gattttaccg cctatatttc agacgaccaa gaattggaga
     2101 acactcttgt tcggaaagga aaagtcgtat taaatcctgg catcacgttt ggtccttccg
     2161 gtcatgggca tatgcgcttg aatattgctt gtccagaaga aactttactg gaaggactta
     2221 atcgaatcaa gtatgcttta gagacaatcg attaaatatt tattcaaaat aaaaggacga
     2281 gaccttacta ggtttcgtcc tttttcataa ttactccgta ccataccaaa ctattctgaa
     2341 atttcaattg tttcaatcac tacatcatgt acaggtcggt cttgtggacc acgttgtaca
     2401 tttgcgattt catctactgt gtccatccct tctagtacat gtccaaagac tgtatgtcgg
     2461 aaatctagcc aaggagttcc gccaccttta tacgcttcaa tgatttctga tgggaatcca
     2521 gcaccttcta gttgtccaag catattagct ggcacatttg tgttatttac aataaagaat
     2581 tggcttccgt ttgtgtttgg acctgaattt gccatagata aagctccgcg aaggttgaac
     2641 agttcacgac tgaattcatc ttcaaaactt tcgccataaa tactttcgcc acccattcct
     2701 gttccagttg gatcgccgcc ttggatcatg aaatcaggaa taacgcggtg aaaaatcacg
     2761 ccatcataat aacctttttt ggaaagttca atgaaatttt gaaccgtctt cggtgctagt
     2821 tcagggaata attgaacagt aatgtcacca cggtttgttt tgataacagc ttttggtcct
     2881 ttttcacttt ttaaatctag ttgtgggaat gccatattgt ttcctcctaa tatttgcata
     2941 cttcataata ataataacat aatacctaac ctctcgtaaa aatactagat ttgttttctt
     3001 ttatttttca tcactagata cattctataa cttaccgttt cgatttcgaa ttcaaacatt
     3061 tttgagaagc attctcaatt aaattctgct atagtgtgaa aagggaggaa tttttatgga
     3121 aatctatgac attacaatca tcggtgcagg tcctgtaggg atgtttgccg ctttttacgc
     3181 aggaatgcgc caagctaaaa caaaaatcat tgacagtcta cctcaacttg gtggtcaatt
     3241 agcaactttg tatcccgaaa aatatattta cgatattcct gggtatccag caataaaagc
     3301 aagtgaatta atcgatcaat tagaaaaaca attaactact tttaaccata cattccattt
     3361 aaaagaagaa gttctctcgc ttactcgtga agacgaagtt atcgagatta ctacaaataa
     3421 aggcatccat tattccaaag cagtcatttt agctttagga aatggttcat tccagccaag
     3481 aaaattgaat ttagataatg ctgaatcatt tgaaaaccac ggattggatt atttcgtgaa
     3541 tgatttgatg aagtatgctg gaaaaaaagt cgctattgct ggaggtggtg attctgctat
     3601 cgattggacg ttgatgttag agcctattgc aagcgaagtt tacttgattc atcgtcgccc
     3661 ggaatttcgt gcgcacgaac atagtgtatc acgcttgaaa tcttcatctg tcaacttgct
     3721 tactccctat ctcatcgatg gattatcagg caataatggg gaactgacag atatccggtt
     3781 aaaaaaagtg aaaagtgacg agacgattga tttgatgatt gattctctga tcgtcaatta
     3841 tggattctct tctaatttag aacatctttc ttcttgggga cttgatagta ccagaaatgc
     3901 cattactgta aaatcagata tgtctacttc tattccaggc gtttatgcag ctggtgacat
     3961 ctgtacctat gaaggaaaag tgaaactgat tgctactggg ctcggtgaag cacctactgc
     4021 agtgaacaac gctttacact atattaatcc aaaggaacga actcagcctg gtcacagcac
     4081 aagcttatac gataaaaatc ttcgtcctag ttgatttttt atatctcttt gcatttcaag
     4141 ttaaacccga aagcagtttc ttgcattttt ttcatgttcc gttaacatga aatagagaaa
     4201 gaaaaaataa agggggagca ataatatggt cgtcaaaact gagactttag aagaaaaggc
     4261 aagagaacta ttgaaggaac gtggcgtaac tgtagaagat atcgcacaac ttgtcatgtt
     4321 tttacaaaaa gactatattt ctgatttgac tttggcggat tgtactgaaa gtgtcaacag
     4381 cgtgctaaca aagcgtgaag tacataacgc cattatcact ggaatccaat tagatatatt
     4441 agcagaaaag aaaaaattga tgagtccgtt gcaacatatt atcgaagatg acgaaggcct
     4501 ttacggtatc gatgagatcc tagccttgtc aatcgtgaat gtttacggta cgatcggctt
     4561 tacgaattat ggttatatcg acaaggtgaa acctggtatt ttaaaagaac taaacagcca
     4621 cgatggcgtt catgtccata ccttcttaga tgatatcgtc ggagcgatcg ccgctgctgc
     4681 tgccagtcga ttagcacaca aagaaccaga aaaaagaagt cgccttactc aataattcaa
     4741 caaaaaaaga aagaaaacgt ctatttagac gagttctttc ttttttgttt gtttcaagct
     4801 attttttcct aagaaaagaa aaacagcaaa aaataattct aataatacaa aaaataagat
     4861 aaagctatgc atgaaaaatt cttctggaag tacattgatg attggatcag taacgggatc
     4921 gaataaccag tcatcgttgt tgaaaaacgt ttcatgaaaa aggataaaaa agcgatcaaa
     4981 tcctatcatc atgaaaaaac cgaaaacaac tggcagtaac atgccgattt gaaacggacg
     5041 aatcaaccgc caaagccgat cattcttttg caaatatttg ataaacaaaa tactcgggat
     5101 caaagtgatg aaaaacacag cataatccac taaaaacaaa attttcactt cataaaagtg
     5161 atgggctccg ctagcagaaa ctgggaaatc tggcaagctg aggatggttt tgaagggatt
     5221 attgagataa ttcattaggt gatcaaaatt tttcagtaac gtctcttgat ctaaagaagt
     5281 ataatccaaa atttgcaaat gtccgatatc aaatacgtat agtggtcgaa agttgatcgt
     5341 cacaccaact gccagactga tcagtgtcag aaataggctg aacaatccag catattccat
     5401 ccactgccat ctcgaatttt tcatattaga agctccattc atccagtgat tgtttcacat
     5461 aggtagctgg gataggcaac ccaggtacat cttcttcttt tgtaaaacct gataaaacga
     5521 gcaaagtatc tatattattg cgtatcccag cttggatatc tgtttcatag ttatctccaa
     5581 ccatgattac ttcttctttt tgcagcccta atacttcaac ggctttatcc ataatgatgg
     5641 cttctggttt tccgatgtag atcggcggcg tttgggttgc tgtctctaca aaagagatga
     5701 ctgaccctgc tccaggaaga aggccgcgtt cagtaggtat attcttatct gggttcgttc
     5761 caatgaaagt agcacctttt tgaatagcaa gtgttgcttt aactactttt tcataggtca
     5821 gataattatc taagcctacc actacatagt caggagcctc ttcttcccat gtaaatccgg
     5881 cagctaaaat caagtcaatc aatcctgctt ctccgataac atagactttt ttcccttttc
     5941 catctgcttt cataaaatcg attgttgcca aagtagctgt gtagactgtc tctggagcta
     6001 catgaatgtc aaactcatct gctagacgat tagctactgt ttctggtgac ttagtcgtgt
     6061 tattggtaac aaacaagaaa ggtaattttc ttttttgcaa ttcttccaca aatcgtttac
     6121 cggccggtat gggttgtgtc ccacgataaa tcgttccgtc taaatcaatt aaatatcctt
     6181 tatagtccat tcatccacta ctcttccctt tttctgatcg taaatgctgt ttttgttttt
     6241 tctacttgtt taggttgttc ttgttttttt actttttcag aggtttttgc tgactctgtt
     6301 tttcgatttt taataaccgg ttttttgcga ttagatgcga ctggtccttt tttctcatcg
     6361 atatacgcac gattatggtt ttttttcttt tttcggcgct gagttgtctt ttctttttta
     6421 ccgccgattc tctcgatgac aaaatacgca caaccaaaat tgcaatattc gtaaagatag
     6481 tcttccaagg aatcgattcg ctgatctgga tgcgcccgtc gattatccgc ttggaaaaat
     6541 ccttttaggc gtagttgttc ataaccccaa tctcctacga tataatcata tcgggctagt
     6601 acatcactgt aacgttctcc aagcttttct gcattaaatc cttcacgata atcggacaca
     6661 agccgataat gtctttcacc tatcaaaaaa ttactgtcat ctaacagtgt cactagctcg
     6721 cctttgactt tttctgcttt aggctcctca acgatttctt ccaaagcagc tgtcagttct
     6781 tcggtgactg ttgtttcctg ttcagctgat gattttttct tagcaatttc tgttttatgc
     6841 tcaacgttct gctgttcatt ttgttttgca gacatgcgtt caccacctat cttttttatt
     6901 ataccttgtt tttatttgat tgggtagtga gctttcaagt aatctcctac cacacttcgg
     6961 ataaattcag ggaacaaaaa ttcatttttt ccagcaatct caatcgttgg aaaaaatgga
     7021 acaaacatca aatgatctac ggtagtaaaa gtataatttt tattcagatc gattggcttg
     7081 ttcttccaaa agacttgatg gttaatagaa tcatatgtga ttccattata gaccatctgc
     7141 ccaaaaatct taccgcgaaa tcccattccc ttcataggat agttacgtaa aaagttacga
     7201 tttttttcta tttctaaaac taagcggatc agatcatttc cacccaatgt gacatttaac
     7261 aagtgcatcg gatgcggcaa cgcagtgtga agctgatcct gattgacccg cccttttgga
     7321 atttcagtta aaaacagacc actgtttaat attgctgctt ccgtctttcc tcgttctttc
     7381 accgcctcaa aggctacttg gataaacgaa tgctcattga agatatctag actcagagaa
     7441 aatggcaaat ctgctacttc tttttctttt aataattcat gcccacgttc tagataacta
     7501 tttatttctt gttcatcttc tgtaaaagct gtcattgttt cggtagggat ggctttggct
     7561 gaatgcttaa ggattttttt ctcttcatcg atggttaaat ggacttcacc gacatactgc
     7621 ccgtactttc cagcggctgc tatttggaca tcattgatta cgtgaccttc tctgaataaa
     7681 tgatgggtgt gcgatcctaa aataacatca attgctggta tttcttttgc aatttggaaa
     7741 tcatcctgaa tcccgagatg actcatcaaa actaaaacat ccacttgtga acgtaaatct
     7801 tcgactaatt ctggcaaaat atcataagga ttccgtatgt cccagccatt cggattataa
     7861 gtaagcggaa atggagcagt aaatgcaatt agtccgattt tcgtctggtg tttcgttgta
     7921 atgattttat atggtttcgt ccatgccggt ggctggagcg tatttttatc gaataagtta
     7981 tccaggatga tatcaaactc agcatgatcg tataaatgat tcaactcttc tttggaattt
     8041 cctacgcctt cattgtttcc tatcgtcgct gcatcataac caacttcatt catcagcaag
     8101 atattttcct gtccattcgt tgcttctgac aatggatgcc aacgatccac aaaatcacct
     8161 agatctactg caatcgttgt cgttttttct ttcttttcgt tttctcgttt acgttgatcc
     8221 aaaaaccttc tgatccttgg ccaattttct aaatgagaat gcagatcatt cgtatgtaaa
     8281 atcactattt tttccatgta ccctctcgcc tctctttatc tttatcatac cataggcagc
     8341 tttttcttcc attgaaacaa gctctatttc agagtttttc ctgccttgaa gcaaattgta
     8401 taagtacaat attcgataaa tcagtctggc taaaaaatca ggaacattca tttttacagt
     8461 actataaaaa taaaaaaaac agacccgaaa gtctgttgtc gtttcattat aatcagacac
     8521 atggcggaac ctgcttagtg ctgcttcctt ccagacctga cacgcttcac aagcccacca
     8581 ttgtcctagc ctttcggcaa agaatagtat accttatttt tcattcgttg accagtcttt
     8641 ttttaatttc ttttttcgct ggcggagctt tcggaaaaag tcagtcaaaa tcatcccaca
     8701 ttcgtcttct aaaatgccac cttctacata tgcttggtgg ttaaaccgct cgtcctccaa
     8761 aagattcatc aaggtaccgg ctgttccgcc ttttggatca tacgcgccga aataaacttc
     8821 tgctacacga gacaaaatca tcgcgccgct gcacatagga caaggctcta aggtaacaaa
     8881 aagtgcagca tcttccaagc gccagcttcc cactttttca caggcttcct tgattgcaag
     8941 catttctgca tgtgtagtag catcttgcgt ggtttctcta aggttatatc ctcttccaat
     9001 cacttttcca tccaatacaa ccactgctcc aatcggaact tcatgaagca tttctgcttt
     9061 ctttgcttct gccaatgcaa ggcgcatcca tttttctttt tctactgcat caagatttgt
     9121 atttaccaat tacccttccc tgcctttgtt ttttcttgtt ttactttagc atatgataaa
     9181 aaggctgtaa ctaacttttt tttctatggt aaaataaata caagaggtga acaagatgaa
     9241 aatggataaa ttacttgctc tttacccaca agcacagaaa aaaaactctc cagcatcaga
     9301 agcggatatc ctttcttttg caattgatga tgcttttatc tggatcaaac aagattcatt
     9361 atctcaacaa gaaaccagcc ttttgaaagc gttattccct gtaatcaacg atcaaaaaaa
     9421 acacccatgg tatcgctatc tttttaaaga tgcttcttgt gttcaagaaa aaagctttcg
     9481 tttgatccag cttcacgtcg aatcaaaagg agaattctta aaaaaagaat ggcaagatac
     9541 tattttggaa ttatttcgct attctgctga tttctttttt tatacagaca cagatgcgct
     9601 cttagtcgaa caaataaacc gtgattatct tgatggtact gaaatcaacg gtatttttct
     9661 atctttagat gctgattttg atacgacaac gtctgtattc gttggttctt tccattcacc
     9721 tggcaccgac ctcgtccctt tgtttgctga agaaagaaaa atttttttaa cagagcaaaa
     9781 caatctttat actcacagcc gtactttttc tatgcaagat gtggctttac attactatac
     9841 aaaagatact gtaaaagata gtaagttgat caaaagttac atagaactga tcaaacaaaa
     9901 tgaaatggaa gtgatcattc aagaattatg gaatgaatta ggcaatgtca gctctgctgc
     9961 aaaaacactg tttcttcatc gtaatactct aaaatatcga atcgaaaaat ttcaagaaca
    10021 gtctggtttc aatctaaaaa aagccaacga tttactgttt tgctatcttt tattgcttca
    10081 tgaacataat taaagataga ttaaaggggt tgtgacaata attttgtcac aaccccttat
    10141 ttcgaatgaa tagtgttcaa aagctagttt ctcttacatc attttttcaa caaaatgcat
    10201 tagccggtct tttacatctt cggttgaatc tttgattgaa tcaccataaa cagagatttt
    10261 cttttttcct gatttgattt ttgctaacat tcccatcaaa gaatccaagt cactatctga
    10321 aagaccatct acgatttcta gtaatttttc atttccatca aatttgtcat agacgaattt
    10381 ttttactttc gtccgagtag tcatatgatg gatcttttcc attacttttc ccgatacaac
    10441 gactgctgtc gctacactgg cagcggctgc tacgcttaat ccgattgtta cttttgttga
    10501 tgttttcata agtatcagct cctttttatc tatctccatc atagcataga caaaaaaata
    10561 aacgaactaa tccactcagt gaaatctcaa tcctttaggg aaaaagttct ttactgttcc
    10621 ttttacttgt ttcccaaatc cgacaggtat cttatctaat actagcaaaa cccatccttg
    10681 attcccatca cgttgaaatg tttctcctgc tgtgtaagat tgccattcct tttgagtgat
    10741 cggcaagcaa ggtatattct ctatcttttt tgtagcaagc gctaatgcat agctaggttc
    10801 aaatcgattc tttttgaaat ctcctaaatg caaacctgta cgtacgactt ttaaaccatc
    10861 gagagaaggg gctaactcag gaacttccca aagatggtcg ttgaacacta aaagtcttcc
    10921 agtagcttca taatgaaaat catttgaaaa ctccgtccag agtttttcct gctcttttgt
    10981 catttgcact ttactttttt ttcgtgtctt cttttcttta tgcatctgat tttgtccatg
    11041 aaaagtcagc tttgcaacaa aatgtccttc accctgatct ttatgaggcc atatacgtat
    11101 tgttttttcc aaaccagcta cgcttcccca ttcagaacgt cctgaactaa cggattgagt
    11161 caaaggaatc tcctcaatcg ttactggata attttctaca agccagctaa tgatctcttc
    11221 attttcttct ggtgcaaacg tacaagtcga ataaatcagt tgtcctttat tttttagcat
    11281 ttttattgct gaagacaaaa tctcttgctg acgtttttga cagtatagcg gagattcttc
    11341 tgtccactct tttattgcat ttggatcttt tcggaacatt ccttctcctg aacaaggagc
    11401 atcaaccacg atccgatcaa aaaagcccga aaaatgaggg acaagttctg cgggtgcatg
    11461 atttgtcact attgcgttcg aaactcccca tcgttctata ttttctgata aaatcttcgc
    11521 acgtttcgga aagatctcat tcgtcactaa taaccctttc cccttcattt gagcagctaa
    11581 ttgggttgat ttaccaccgg gagccgcaca caaatccaaa actttttcac cgggttttgc
    11641 tgcagcagct gttccaacga tcattgcact tggttcttgg ctatattcat acccagcttg
    11701 atggaggaaa gactttccat tgacagtgcc aagaaatccc tcattgctgt agggagcggg
    11761 ttgtaattct tcgctatggt aagtttggac catgtccaat cctgccggtt ttagtggatt
    11821 ccaccgaaat ccttttttca cgcttccttg ttcaagcgca gaaaaaaaat cggatgcttc
    11881 ttcgcctaaa agaagacgat attttttgat aaattgctgt ggtaacgttg tcgcttcctc
    11941 cttcattcca atgtttcgtt agacatttta caatcttttt tacgaaaaat aaaggtggct
    12001 gatctacttt ttcatgttgc tgcctaaggt gactgttcct ttaagatatt ttccaagtat
    12061 tcatccgtca cctcgttttt atcccaaaca gcgttcatca tttcaagaaa atcttgagaa
    12121 gaatcatctg agcgcaaact tcccaatgca ttaagccata ccaattcaag cgacttttct
    12181 tctttgatct tcgtgtgacc tttgacatat gagaaaaatg gccgcttaaa tagcgaatcc
    12241 atgtctaaat cagattttgc aatgatttca cgaatacttt catggataga aaaaaaatct
    12301 ttcaataagt agacaatgct taaaacatca tcggaactgc attcagctaa gtagttttca
    12361 aaataaacag tgatttcttc taatatcttc tcttttaacc cgctgattga tttgaaatgt
    12421 gtataaatcg ggtatgtaga acaaccgcag aactctgcta actctcttgc agtaattgac
    12481 ttcattccat attcttccac gtataccata cttttgaaca gcaaatattc tttactataa
    12541 tttgttactc tccccatcat tcttacctcc gtctcttttc atctacccat aaataaataa
    12601 cataaataat tctaaaaata ttattgataa ttatcaaaac atatttagtt atatccatta
    12661 aaaatcaatt tttccgcctt aaaaacaaga atatcttgac aaaataccct aggaaataaa
    12721 caatagaata aacaaggtaa caaaaaaatt aaataacgtt atataaaaaa gaaggcgaca
    12781 ctctgtgaga ataacacaga ctgaaatgac tttccactca cagtttttgt ctaactatat
    12841 acaaaaacag aataaaaagg aggtatttat gctaattgaa gcaagaaaaa aagagttact
    12901 tgttctacaa atggtcattc aaaaaaataa ttgttcttta caagaattat ccaatgactt
    12961 acatattcca aaacggacaa tcaaagaact gatccgaaaa ataaatgtat cactcaagca
    13021 atttttattg attgatgaat ttatttattc taatcataaa ggagagatcc aaaccaaaga
    13081 aaccgatcaa actaaaaagt tagctgtttt ttctaagtta aaattatgct acctaaaaga
    13141 atccaatcgt tttaactatt tgatgttact aatcaattat ccacaaactg ctgtaccaaa
    13201 aaaatattta cttgaacaac tttatatatc gccctcctat ctcgaaaaac tgacaaaaca
    13261 gctcaatcat tccttaaaaa aattccattt acggatccta tctgtaaaaa attgctgtac
    13321 tcttgaagga aatgagttat ctatccgtct ctatctttac ttttttcttt cagatacatt
    13381 tggaggtaac gattggccat tttcctcact tgaattaaat gcactaaaaa tgaacagtac
    13441 agatgagcaa atagaaaaaa tttcaaaaca agaaaaaaat atctacttgc tgttaacttt
    13501 attcatatta cgaaacaaac aacaggcata ttttccaaaa acttcttctg aaatcactga
    13561 attgatggac ttattgcagc atacacatga ttattcagaa tactttactt ctttttcagt
    13621 gagtcgccct tgtgaagaga cagaaacaga gagagcctac tttaattatt ttttctattg
    13681 tttttttcca aatcaaatct cttttcgaca aaaacaggag attggttttt tattttccac
    13741 aagcaaaaac cttatttgtc gacaagtcgg tcaattgatg acttacttaa ctcaacagtt
    13801 tcctctcaac catttagaaa ataaaatacc aatttatttt tattttcttg tgttggcagt
    13861 tgtttttttc gaactgttgg gtgaatccag cagtagtttt tctacacttt gctttccaga
    13921 agccacgtcc aatcattcta gttcttttat acaggaaaag aaattaagag aaaaaatcga
    13981 tacaattttt tcaaaaaatg gggaaagaaa ccaaatcagt caatacctta gtcaattgat
    14041 ccaacactta ctacaaacag aaacgaaaga gcagttactc atccatttgc agataaccaa
    14101 acagtttaca ggaaattatc atcttcaaag ccatttgcag cagttatttc gcaaagatgc
    14161 tgttaaaatt accgcatatt cagaacatgc tgatttgatc attacagatc agcatgagaa
    14221 aatttcagat gaaacggaga ctttctatct ggatacaacg cacaatacag tgttatttgg
    14281 caagttaata aaagcggttc atgaactgta tatgaaaaaa ggttatgaga acaaaacaaa
    14341 gaggatgtga caaaattctt gtcacatcct cttattccgg ctctataatt tatgggactg
    14401 atgaatcaga attgattctt cagccccatt tttattttag gtattaaaaa acatatcgtt
    14461 ctctagtatg attaaatcac gacaaaataa acaactgaga ggacgatatg ctatatgaat
    14521 gattctatca aaaaaatgct gcgattaata gagaaagatt tgatgattac agaggtctct
    14581 tacgagacct ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc
    14641 catacctgta gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg
    14701 aaaaatggga agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt
    14761 atgcgcttaa aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa
    14821 agttattttg ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct
    14881 ttactgactg aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc
    14941 accgttattc gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt
    15001 ctccccagag tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg
    15061 agctttattt gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa
    15121 ttgcctcgat taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta
    15181 gtcacagaca tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa
    15241 gtagtgattg atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt
    15301 atccgtgaaa tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa
    15361 aagaactggc gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca
    15421 tggaaaagtt ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga
    15481 ttgcttggct tttctacgtc cctgaaggag gcgtatccct attttcatga attagttgaa
    15541 gcctttcgag acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg
    15601 ttagatgacg gctttcggga aaagctccaa aaccttctga cctatgaaga aggcatcacc
    15661 aacgcgatga tctatcctta caccaatgga aaaatagaag cgaagaatac ccatataaag
    15721 acaatgaaac gagtatccta cggatttaaa tcatttgaga acatgagaat tagaatcttt
    15781 ttgatcaatc aattaatcaa a
//
LOCUS       DAHUVV010000062        15782 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.063, whole genome shotgun sequence.
ACCESSION   DAHUVV010000062 DAHUVV010000000
VERSION     DAHUVV010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.063"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..380
                     /locus_tag="NK767_001900"
     CDS             <1..380
                     /locus_tag="NK767_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6694894.1"
                     /translation="YLSPIENIRLVNKSADESILFELGLDKKQIKRNVMKLSGGQQQR
                     VAIARALVSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADS
                     ADIILELSGKKLKKVNKMNLEVE"
     gene            380..1756
                     /locus_tag="NK767_001901"
     CDS             380..1756
                     /locus_tag="NK767_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6694895.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2042..3664)
                     /locus_tag="NK767_001902"
     CDS             complement(2042..3664)
                     /locus_tag="NK767_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HCH6694896.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     PFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVK
                     GEREKQFAYSVHTSCDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNIN
                     VYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
     gene            4316..5287
                     /locus_tag="NK767_001903"
     CDS             4316..5287
                     /locus_tag="NK767_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCH6694897.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(5365..5601)
                     /locus_tag="NK767_001904"
     CDS             complement(5365..5601)
                     /locus_tag="NK767_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCH6694898.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(5570..5701)
                     /locus_tag="NK767_001905"
     CDS             complement(5570..5701)
                     /locus_tag="NK767_001905"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCH6694899.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(5777..6160)
                     /locus_tag="NK767_001906"
     CDS             complement(5777..6160)
                     /locus_tag="NK767_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCH6694900.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(6193..6978)
                     /locus_tag="NK767_001907"
     CDS             complement(6193..6978)
                     /locus_tag="NK767_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HCH6694901.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            7156..8256
                     /locus_tag="NK767_001908"
     CDS             7156..8256
                     /locus_tag="NK767_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCH6694902.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            8246..9070
                     /locus_tag="NK767_001909"
     CDS             8246..9070
                     /locus_tag="NK767_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HCH6694903.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            9067..9882
                     /locus_tag="NK767_001910"
     CDS             9067..9882
                     /locus_tag="NK767_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCH6694904.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            9879..10709
                     /locus_tag="NK767_001911"
     CDS             9879..10709
                     /locus_tag="NK767_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6694905.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            10716..11747
                     /locus_tag="NK767_001912"
     CDS             10716..11747
                     /locus_tag="NK767_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6694906.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(11854..13127)
                     /locus_tag="NK767_001913"
                     /pseudo
     CDS             complement(11854..13127)
                     /locus_tag="NK767_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            13404..13613
                     /locus_tag="NK767_001914"
     CDS             13404..13613
                     /locus_tag="NK767_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694907.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            13663..14379
                     /locus_tag="NK767_001915"
     CDS             13663..14379
                     /locus_tag="NK767_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCH6694908.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(14281..14801)
                     /locus_tag="NK767_001916"
                     /pseudo
     CDS             complement(14281..14801)
                     /locus_tag="NK767_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            15089..15742
                     /locus_tag="NK767_001917"
     CDS             15089..15742
                     /locus_tag="NK767_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6694909.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT         4788 a         2795 c         3420 g         4779 t
ORIGIN      
        1 attatttatc accaattgaa aatattagat tagtaaataa atcagcagat gaaagtatct
       61 tgtttgaact aggtttagat aaaaaacaaa taaaaagaaa tgttatgaaa ttatctggtg
      121 gacagcaaca aagagtagct attgctagag cattggtatc agatgctcca ataatactag
      181 ctgatgagcc tactggtaat ctagacagtg ttactgctgg agaaataatt aatatattaa
      241 agacattagc taaagataga aataaatgtg taatagttgt aacacatagt aaggaagtag
      301 cagattctgc ggatatcatt ttagaactaa gtggtaaaaa gttgaaaaaa gtaaataaaa
      361 tgaatttgga ggttgaataa tgataaaaaa tgcatttgct tatgtaacta gaaaaagttt
      421 aaaatcatta attatcatat tagttatttt atctatgtca actttaagtc tcattagttt
      481 atctattaaa gatgctacgg acagagcttc aaaagaaaca tttgctaata taacaaatag
      541 tttttctatg gagataaata gacaagtaaa tccaggaaca cctagaggtg ggggaaatgt
      601 aaaaggtgaa gatattaaaa agatatctca aacagatagt atagactcct atgtaaaaag
      661 aataaacagt gttgcagatt tagttgatta tgatattatt gaaactcaag agactcttgc
      721 gaatcaatca cctgaaagag caaagaattt taaaagaaca gttatgttaa ctggggttaa
      781 cgactcaaca aaagaaacaa aatttgtatc agaagcatat aaattagtag aaggaaagca
      841 tttagaaaat gaagataaaa ataaaatctt aatgcataaa gatttagctg aaaaaaataa
      901 ccttaaagtt ggagataaga taaaaataaa atctaattta tttgatgctg ataatgagaa
      961 aggtgcagat gaaacagtag aagtagaaat taaaggtcta ttcgatgggc ataatagcgg
     1021 tgtagtaagt gcagcacaag aactatacga aaatacatta atcactgata ttcatacagc
     1081 tgctaaagtt tatggtaata ccgaagatac agctgtatac caagatgcaa cattctttgt
     1141 aaaaggtgat aagaatcttg atagtgtaat aaaagatctt gggaaattag atataaattg
     1201 gagagaatat aatttgatta aaagttcatc aaattaccct gcattacaac aatctatatc
     1261 aggtatttac tcaatttcaa ataaattatt ttttggctca ttaatatttg caggagttgt
     1321 agtttcatta ttattattct tatggatgaa tgctagaaag aaagaaatag cagtattact
     1381 atcattaggt atatcaaaat tagaaatttt tggtcaattc ctaattgaga tggtatttat
     1441 atcaatcccc gcattcgttg gttcctattt cttagctcaa tatacagctg acaagcttgg
     1501 gaataatata ttgaataagg ttactggtga tatagctaaa caaatagcta aacaatctgc
     1561 atctagccaa ttaggtggtg gggcagaagc tgaaggattt aacaagacat tatcaagttt
     1621 agatatcaat gtattaccta aattcatgat ttatgtagtt atatttatga gtttagtact
     1681 tcttgtatca ttgattattt cttcattcaa tatattaaga aggaatccaa aagaattatt
     1741 aattgacaat aattaaaatt ttcggtgctt tttagcacca aaaatgagag tgtaatataa
     1801 accgtgtaag taaccatcaa tcctaggcgg gtggttgctt cacggttttt atttttctaa
     1861 aataagatca agaggtgatt acacgatggc tagaaagaaa agaaatcctg atgctgagaa
     1921 gttagctgaa tccattttga atacttatca acccgaatct gtagaggata tgcaagatgt
     1981 tttaaaagat gtgtttggtc ccttatttga aaaaatgctt caaggagaat tgaataacca
     2041 tttagggtat gacgcccatt ctaaagagcc aaagaaacat gacaatcgac gaaacggtta
     2101 tggcaataag acgcttaaaa cgagctttgg taaagtaaac attgatgttc ccagagaccg
     2161 ggaggcttct tttgaacctg agttgattcc taatagaaaa cgagatgtct ccgacatcgc
     2221 agactgtaga caaaagctgt tccaaactcg taactcgagc ttggaacagc tttttatcta
     2281 cttcgtttcg aatttttgaa caaaaaaata aaggctcctt gcctttccat agccaggtgg
     2341 ctaatttctt taaattcatt gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca
     2401 agcctcgata cttggtccaa cgcatcccat gcttttcttt tgcatccgca aatattcgtt
     2461 caatcgtttg tttccgtttt ttgtaaattg atttattcag atctgttagg cgaagatgtt
     2521 ccacttcatc catcagatct ccccaaacat gtctcgtgat gactcgttga tgatttttac
     2581 ttatggtaca ttggtttagc agaggacagt tcacacattc ttttggattg gatttgtatt
     2641 ctcggtaccc ttctcgagta accgttgaaa aagcgagcat ttgatcttgc ggacaaatat
     2701 aacaatcaaa atactcgtca tagagaaact cattttttga taatagctcc tttttccctt
     2761 tgggccttga ataaggcaaa caaggtcgaa ggttcaaatg agtcaaaaag tgaacaaact
     2821 taggtgtctt atacccagca tcagcgacaa cacaattgat ttcaggaaaa agtctcttcg
     2881 attgtttgac gagctgaaag gcgacttgac tgtcatgaat atttctagga gtgatgattg
     2941 tgctaagaat aaatccattg tcgtcgcatg aagtatggac agaataagca aattgttttt
     3001 ctcgttcgcc tttgacgtag tagccgcttt ccggatcagc tttacttatt tttctttctt
     3061 tcagcttcac ttccttttct aagtccaaag taaaagggcg ttttccagcc tctattcgtt
     3121 gacgattgat ctcatcttct aattcatcct gataggcttt cgcttcatca tgggtcattt
     3181 ctttcgtaaa cttatgttta ttggcgttag ctttgatgtg ggtagaatcc agatacaaat
     3241 tatcctctgt tacaaaacct gctttaactg cttgttctaa aatataggca aaaatatcct
     3301 caaacaccgt cgtttctcta aatcggcgaa cataattctt tccaaaagtt gagaaagggg
     3361 ggattttctc ctcgaaggag tagcctaaaa accagcggta agcgacattg gtatcgactt
     3421 ccttaatggt ttgtcgcatg gaacggatac cgaataaata ctgaataaac accaatttga
     3481 tcagaatcac tggatcgata cttgggcgac ctaaatcgga gtaagtggct tcgacgattg
     3541 gataaataaa ttcaaaatca agcgcggcat caatctttcg aacgagatga tcttgaggaa
     3601 ctagatcatc aatcgtaagc atcgcaaatt gatttctacc attcgtattg tttttagaca
     3661 tcatagctat cactccttgc attcgttttc ataaatctat tataaaagaa aagactgtag
     3721 acatcatcca taaaatggac tttgtctaca gtctggataa cgtgattttt tgctgtaatg
     3781 acaaggtttt agctgtttca gataccatgt atccaaaaat agtttattga gaaaatagga
     3841 gagtatttga tactaactag taggaaaaaa tgagataaaa agtaaatgga atcatttaag
     3901 gtaatctgaa ataaaaaata tacagccctg tggtacttta cctacaaggc tgtatatatt
     3961 ttttactctc agcgtttttg actccgtgtc tgactccgtt ttattgattt tgaatgttat
     4021 tcaatgacca tttaacaggt caaatttgat gtttttgatt ccgtatgacg ccgtttgata
     4081 tctagtgata tagtgatttg agtctacatc gaataaggga ataaaccaat aaaaaatgat
     4141 ttagaatatt aaaataacaa aaaagaggac tacctgtatt agtcctcttt tttgaccaga
     4201 ttttgaccag tctattttac atcctagtat atttatatga gctaatttta tgattaaatg
     4261 gtaagttttt ccttgtgtgt tttagtttaa actagtgtgt aattcaaaaa tctatatgga
     4321 aggaacagta ttaagaatag aacagggttc tctacatgat ggtgctggtt tacggaccgt
     4381 tgtttattta aagggctgcc cattacgctg tgcctggtgt tcgattcccg aatcacaaag
     4441 caagcagatt gaaaaaggat ttggccagac catgaccgct gaagaggtaa tggatgagat
     4501 tgagaaggat gctgtttttt actttcactc ggatggcgga gtgaccatta gcggaggcga
     4561 ggcactggtt caggcggatt ttgctaaaga aattttgcaa aaatcgaaat acattggaat
     4621 caataccgta ctggaaacca gcttttgtgg tgcttataat gaaatccaaa aggtagctcc
     4681 ctatgtggat acattgtttg tggatgtaaa aatgtttacc agcaagctcc acaaacaatg
     4741 gaccggctta gataaccaac aaattttgaa gaatattcgt cgctttttaa ttgagtatcc
     4801 taactgcgaa gtgcgaattc gagttcctgt ggttccaggt atcaatatga acctgaccga
     4861 gctgctgacg attgcttgtt ttgtggcaga tcttgatcgg ttcgtaccgt tagaattact
     4921 accgtaccac tgctatggta tgcacggtta ccaagcattg ggactggagt atccgttggc
     4981 agacacgcca gcgccttcac ctaaagaaat gtttgcattg gcagatcaac tggctcgcac
     5041 ggttcctcat ttgctggtca cagctttaac caaaacattt atttatttat tgaatccaag
     5101 ctttatctta tggataaatg aaagacactc actagctgca gccagctcaa gaatagagct
     5161 tttgctgaag tcagtgagtg tctttttacg atgttttttc gaagttaatt tgtcgaatga
     5221 acactggctc aatcggtaca tcttcactgt ttgtcggctg agctgcgatc gctgctacga
     5281 tttctaaccc ttggtatacc tgtccaaaaa cagtataacg accatccagc tccggtagtc
     5341 ctccaagctc caagtatgct tgctttacat tagcaggtca gcctgcgcct ttgtgttttg
     5401 cacaataaaa aactggttgc cgttggtgtg ggggccgtgg tcccctaagg ccaaggcgcc
     5461 attaaaatga taaagtccgc gatgcacttc atcagcgaaa aagccctcat agctggaaac
     5521 agcttcatca ccagaaccat ccaaagcgcc accttgaata acaaattcct taatgacacg
     5581 agccgaaagg tgtgttgtca taaacgcctt gctcagctag ggagcgccag ttggctacag
     5641 ccaggggagc ttggtcagga aataagcgga gacgaatttc accagcatcg gtgaccagca
     5701 cacaaacttc ttcctgctgc ggtttttcta attgaaagag aggaaggttt gcaactgaaa
     5761 cgccttgatc ctgccatcaa atatcgaaaa tccgccactc gaccttcgcc tgatcaccaa
     5821 ggcgagccat tcccaataaa acggtatagg cggtttcatc cgcttgaaag gtcaattgca
     5881 gctgtcctcg taaatcgttg tagtcatctg gggcggctac ttgataaaaa agatgaaact
     5941 gcgcatgcaa atattccaaa ggctgatctt taaaggctgc aaaataagcc tgaacttttt
     6001 cccgctgcaa gctgtcagcc accaaaaaat tttccagttg ttgttttgga acggctgcta
     6061 acaaggatcg gcaaaccgcc tcaattttca acgcatccca atgtcgcgca attaaataat
     6121 ggatgggtgc ctcttgattg tagtcccaca ggttgctcat ttttttcacc actttcttct
     6181 attatatagg aatcacaaaa tcggcagcgg cgtcaggcgg cgttcttttc cttgaatgac
     6241 tagcaacttt tctacctgac agtctgccaa cagctgactc atttttttaa agcccagctg
     6301 ataatcttcg gcaacatgag catccgagcc taaggtaaag agctttccgc cgacactttg
     6361 atacaaagga acggcataac ggtaaaggtc ttcgttgccg tatttcaaga agctcttggc
     6421 atttaactcc atcgccaatt ctcgttcaac aacctttttg aaaatcgtta gcagctgagc
     6481 ctcaaaatgc tccgcgaatt cttccgctgt gaaggtgaag cggcgtaatc cataatcaaa
     6541 gtgagtcaaa atatctcctt ctggaaaatc cgtcaagacc tgcaacatct gatcaaaata
     6601 ttccttggta gtttgaatgg gatctttggt taaaaccaca tcgtccatat aatcaaagcg
     6661 gccattttga tgaatactta ccagctttaa atcataagga tgctgagcga ggtaatccac
     6721 gatccgctct tcttgtcctg gaacaacgcc aatctcgata cctcgcagaa atttggtgga
     6781 ataggtttct tttaaagagt ccagtttgtt tatataggct ggataatccg gaatgtcatc
     6841 ttttccggta gatggattgt ttaaatcaaa gtgatccgta gaaacgaaaa actccggctg
     6901 ataggctaaa tagttttcaa atttttcctc tgaatcaaag gaaaaatacg tgtggagatg
     6961 ctgatcataa tactgcatag ggagcctcct tttttctact atcataccgc aaaagcttta
     7021 gaaggttaac gggaattcgt cgaaatttac aaaccatata caacctttac attgttcatg
     7081 aaaccttttc aagaaactga ttctctcatt acaaagcttt gttagaatgg aattatcgaa
     7141 aggggagaag aatgaatgaa aatgaatgaa aaaattcgta acatgactat cggtttattt
     7201 tgtgcagcaa cagtaagtat cctgctgaca ggctgcggct caggtgaagc aacgaagcaa
     7261 tcggctgcgg cagacgactc tttagatacg attatagaaa aagccaagga agaaggagag
     7321 attgccagct tgggaatgcc ggatacttgg gcaaattggg taggcacttg gaacgacctt
     7381 gaaaaagaat acggcttaag tcacgtggat acggatatgt ccagtgcaga agaattggct
     7441 aagtttgaat cagaaggaaa aaatggaacc gccgatattg gtgatgtcgg tatcagcttc
     7501 ggtccgttgg ctgaaagcaa agagttaacc ttaccttata agaccaacta ttgggaggaa
     7561 attccggatt gggcaaagga tgatgatggg gattggttgt taagctatac gggaaccatt
     7621 tcctttatca ccgataaaga gaatgtgaag caagcaccga ctagctggaa ggagctgcaa
     7681 gagggtgact atcaagttag cgtgggggat gtgacgattg ccaaccaagc acaatttgct
     7741 gtattggctg cggcgattgc caacggaggt gacgaaaagg atattcagcc aggtctgaat
     7801 tactacaaaa aaatggcaga aaaaggccgt ttgtccaccg ttgatgccag cgtagcaaat
     7861 cttgaaaaag gtgaaatcga tgtggcagtt gtttgggact tcaatggact aaattatcgt
     7921 gaccaaattg actccgagcg ttttgaggta actgtgccaa cagacggctc catcattagc
     7981 gggtatacaa ccttaatcaa taaaaatgcc cctcatccca atgcggcaaa gctagcacgg
     8041 gaatatattt tatctgatga agggcaaatc aatttggcaa agggctatgc gcgacctatt
     8101 cgcaaggtag agctgcctaa agaagtagct gaaaaattac tgccggagga tgcttatgaa
     8161 gcggcggtgc cagttgaaga caatgaagct tggaacaaga cctctgtaga gcttccagag
     8221 ctttggcagg cggaggtact gacctatgca aagtagactt gacaaactga ttgtagttat
     8281 actagacggc tgtcgctacg acacagccgt cgcccagctt gggttcatga atcatttagt
     8341 tgagcaccag caagcaagct tattgaaggt aaaaagtgag ctgcccagca attcacgtcc
     8401 gttatacgaa gtactgatga ccggcgttcc agcctatgaa aatggcatct atagtaatta
     8461 ccatgctcag cgatccaaag agtggtcgct gtttgaatta gttaaggaag ctggcggaaa
     8521 aacaggagcc gccgcttacc actggtacag cgagttgtac aatcaagccc cctataatcg
     8581 tttggttgat cgcatccagc atcaggagga gcgactgatt cagcatggca tcttttatag
     8641 tgaggacact taccctgatt cgcatttatt tgcggatgcc cattatttag tgaatcagta
     8701 tcagccagac tttttggtcg tacattcgat gaatattgat gatgtcggtc acaaatttac
     8761 cgccgattct catgaatatg cagcagcggt taatcaggcg gacagattat tggctattta
     8821 ccttcctgaa tggctgcaaa aaggttatca agtcgtggtg actgcggatc acgggatgga
     8881 tgcgcacgga ctgcatggcg gcagcttggc tgcacatcgt gaagttcctt tttatttgat
     8941 ttcaaataaa cgccaaaagc tgaaggaaca aactgtacca cagctgctgg cggctccatt
     9001 gttttgctgg ttattaggaa ttgcaccgtc agagaaaatg caggacattc aagaattgat
     9061 gaggggatga aagagatgaa aaaacgcttg gttctggttc ctgcctcctt tatcttctgt
     9121 gcatttttac tgctgccgct gctttttatg gttctgtgca gcttgcacag tgatttaact
     9181 aaccagtgga cattggccaa ttacagtgat attttcagca acaaatatta cacacaggcc
     9241 tttgttaaca gcgtactgat tgctgtcagc tccagtgtga ttgggttaat cgggacgttt
     9301 attttatgtt tatgcttaat gaatctatcg actaaattgc aggagaaaat cacctttgtt
     9361 tccaatttgg cagctaattt tgctgggatt ccgctggcat tctcttttat tattttgttg
     9421 gggaatgtag gtgtgctgaa aatgattttg ccatttctag cggacttcaa tttgtattct
     9481 tggtggggat tgaccatcac gtatacctat ttccaaattc cgttaggggt cttgtttttg
     9541 tatcccagca tgaaggaagt gaaaaaggaa tggctggagt ccgcacaatt gctgggcgct
     9601 tcatcagcgt atgcttggtt caaggtggct ttgccttttt tgcgaccatc gattgccagc
     9661 acctttgtca tcttatttgc taatggcatg gggacctatg aaacagcgta tgccctgacc
     9721 agcagtaatg taaatctttt aacgattcga attgcggctt tagtgtcggg agatgtgttc
     9781 gccaagccta atttaggcag tgcgttggca gtcgctttag gtgtcctgct ggtaacggca
     9841 atgttgatta ttcaaaagaa aggcaaggtg gtgcagacgt gaaaaagtta ataaaaccga
     9901 ttttgttctg ctttgtcctg tattacttgc tgccgattat tggaacgatt ttgtatgcca
     9961 gctccactaa atggtcaaaa tcgcttttac ctagtgattt taccttgcaa tggttccaac
    10021 agctgctgac ggacagggaa tttattgctg cggtgggtcg ctcactttta ttagcaggtg
    10081 tggtcttggt gacgattctg ttgttgatga ttcccaccat tatttggatt catttgtatt
    10141 tccctcgttt gaatcgttgg ctggaaaagc tgctcctgct gccgtatgct ttaccaggag
    10201 tgattttggt aacggctttg cttcgaacgt atgctgaaac tggtatccca atgtttgtag
    10261 tgttggtagg tgcattgttt attacggcgt tgccgattgt ctatcttagt cttaacaacc
    10321 agatgcgctt aattaatctg aaggaattag tggatgcagc agaaaccttg ggcgccccga
    10381 tgtctacgat tattatccag gtgctgtttc caaacatccg tatcggtgtg accctagttt
    10441 ctctgatgat tttttcttcg gtttttgggg agtacatgct gacgaacctg ttgattggcg
    10501 gtcgatttca gacggtacgc atctatatgc tgcggcggat gaacgaaaac ggccatttgg
    10561 ctagcgctgt gatggttttg tatttgctgt ttatggtggg aattgcttta actaccttta
    10621 tgctgactta tcgacaaaag caacagctgt cccttaagca gacgagggaa gagaagcagt
    10681 cgaaaaagaa gcccttaaag gagttgtgac ggaaaatgtt tttagaaata catgatctca
    10741 atctgttttt tgatgaaaaa cagattttag ctattgatga aattcagatt gaaaagggtg
    10801 agctggttac tttactaggt cccagcgggt gcggaaagtc tacgttgctg cgctgtatca
    10861 cgggattgga acaaccaaag agcggtgaga ttgtgctgga caatgagaat attgccgata
    10921 agcctacgaa ggatcgcaat atcggctttg tttttcaaca gtatgcattg tttccaacta
    10981 tgactgtttt tgaaaatgta gcctttggtt tgaaggtaaa gaagctgagt cctgaggtga
    11041 ttcaggaaaa ggtttttgag atgctatcat tggtggatat gacggagcag gcagataaaa
    11101 atgtccagtt tctttctggc ggtcaacgac aacgggtggc actggctcgc tcattggtca
    11161 ctgaaccgaa agtcctgctg ctggatgagc cattaagtgc tttggatgcc cgaattagaa
    11221 aacagctgca gcgggatttg cgcgctatcc agcaatcgct agggatgacg atgatttttg
    11281 tgactcatga ccaagaagag gcgatgcgaa tcagtgatcg gatttttgtg atggaagctg
    11341 ggcgggtggc tcaggtttcc acctcgaagg aattgtatca gaatcctcag agccgatttg
    11401 ttgcggaatt tatcggcaac tataatcgtt tcgaatggta tgagctgaat cagtataccc
    11461 acgagttttc cgctaagggc aatcgctgca tttattattt aagaccggag ctgattcaat
    11521 ttgaaccggt tcctcagggg gttaagattc cggttctatg gaaggaatca tttattttag
    11581 gcaacattca acggtatgtt tttcaaacgc tggagggcaa agaaatttta gtggatcggt
    11641 tgaacgatcg ggaagagaaa attagcgatg ccttatacat tcagctgaaa gatattctag
    11701 caatccctgt ggaaattgat actcagcatt ttgcacaaca aagctagtgt atagtcggca
    11761 aacatttctg gctctttgtc aaataggact gatagagtga tataagaaca tctaaggtag
    11821 aattgatttt ctgctttaga tgttttttta tgtttaataa gtgatcagat gattgattaa
    11881 gaaaattctg gttctcatcc taacccgtaa gctattcgtt tcaatgcttt gatgtgggta
    11941 tgcagatttt ctattttgcc atttgagtaa ggcaactcta aggctaaacg aatgccggat
    12001 tcatagttta taaacgcttt ttttatatga tgcagttctc ttggaaccga cttgggcatt
    12061 gtttttacaa gctgaaagaa gatgtccgct tctttgtcat aaaaagcttg aagaatttct
    12121 tgatagtaag cataagctat tcgtagttca tcagaagtag aaagtagacg ttctacgact
    12181 tcactttcgg ttaaataggc ccaaccaaaa ttacgccatt ttgtccattt atcataaaca
    12241 agcgtgtcct gtcgtttgat cagaagtcga gataaagcct tcacttgtcg atagtgtttg
    12301 gcttgtattg gatcgtttcg gtcgaagctt ttcatgactc atagtcaaat atccttaaaa
    12361 ctgcgaatga ggtgcttgac gatgtgaaag cgatccacaa cgagtttggc atgaggaaag
    12421 cactctttta tcagcaaagc ataagaggcg ttcatatctg ttacgagtat ttttacgttt
    12481 aaacgtgcac gtcgttcaaa ttgcataaaa taagaaatga gatctttctt cttacgtgaa
    12541 ggaagaatat ccagtaattt cccagtatcg ccatccatac aagaaaaaga atagggaccg
    12601 acttgatttt tcaatgaacg aaattcatct accaatgaac agcgaggcaa ccaatttatt
    12661 ttcacgtttg tcatggggag cagagaagcg agtgttcgcg aaacggttga ccaagaaacg
    12721 ccgcaatgtt tagaaatcgt cgtcattgct tgtttttcag tcaattcctc caatatttta
    12781 taatgtactt gtttggcgat aaaacagttt ttttcaacaa tgtaggtatt cgccgtaaaa
    12841 taggtgtgac agccacgaca gaggtaccgt tgctttgcca aagcaagata agtccgttga
    12901 ttcgcacact tattgagaag gaccagcgat tttttctttc cgttcttaac gatcagccca
    12961 tgttgattca cctgtttaca ctgaggacag ggctgaatcg ttttaatgag cgtggcagaa
    13021 acaacgatac tgttgattga atgtattttt ttcttctcga catgagtgat ttgaatgttt
    13081 ccatctttga ttcctagtaa tagtttgata agatgaagat caggcataca gaacgctcct
    13141 ttgattgtgt tgtggtgatt caattctaac ggagcttctg tatgttttgt ttttttatgc
    13201 tcaaaaggac tgatagaatg atttactatc agtcctagaa attatagcgc cacatttctt
    13261 tcacctttga cttttcagac ggcagttttt atgctgatac tgtagagaga gcaggacaca
    13321 gcttttgctt gttttgggta agggggaaag aagatgacca aagcacttat ttttgatttt
    13381 gatgggacga tgattgacag tgaatggatg ttttacgaga gtattcatca ctatttactg
    13441 aagcctattt actggagcag ttcggctata aaattccttt tgcagactat caacaaggtg
    13501 ttgggacggc agcagaactg tccttttatg aagggataag tcaagcggtt gggagaccag
    13561 tggatgctga acattttgag cagcggatca gcagcactgg aaaatcggag tagtctcaat
    13621 tctgaactgc gggagctaga ctattttttt gaagcacatc cgatgattaa ggaaaaaatt
    13681 gattcctttg tgacgatcga tgatcttcag gtcggcaaac cagatccaga aggttatttg
    13741 ttgtgcttga aacggctgag ggttgaacag gctgttgtag ttgaggatag tccagtcggt
    13801 gcccaagcag caccgcatat gacagttgat gtacaagcgt tggatgcaga ttttttcgcc
    13861 tttagcgggc ataaaatgtg cgggccgaca ggaatcggtg tattatatgg caaacgtcaa
    13921 ttgttagaac aaatggagcc agtcgaattc ggcggtgaaa tgatagattt tgtcaatctt
    13981 tacgatagtt catggaaaga gctgccatgg aaatttgaag ctggtactcc gaacattgca
    14041 ggagcaatcg attacttgaa tgagattggt atggagaata ttcatcgtta tgagcaagaa
    14101 ctggttgcct acgttttacc taaattacaa gcgatcggcg gtatcaccat ttacggaccg
    14161 caagatcctg aacatcatac tggcgtggtt gcttttaatt tggatggcat ccatccccgt
    14221 gatgtggcga ctgctctgga tatggaaggt gtcgctgtac gttcaggaca ccattgtgca
    14281 caacggttca tcgacgattt ctgtttgact aattggtcgt ttaaacagtc gatgataatg
    14341 ccgtttgagc ggctccaatg tcgtggaatc tttgagtaag agtttccaat atcgcttgag
    14401 ccgacggtac ttcttctgat tttcagatta cgtgattgat tttcgtacag ttctatgaga
    14461 atcgcctgct tcagttcctt tgttagttga tgatgttcat caactaagtc cgttttcgca
    14521 gaaaaggtct gcccacaatc atagcataga aaacgggtac gttttaattc taaaatagtc
    14581 aacttttctt tgactttcaa catctgagta cgtgtcgtat aggaaccgtt gcggataata
    14641 cgaatcgatc cacagtgttc acaatgggaa ggtgagtcat ccaatctccc tttaataaac
    14701 tgcgcttgaa cgcctttata ttctttttca atcagccatt cttcatcaaa aaataaatgt
    14761 ttatctgtta atccgagtaa ttttctagta tgattatcca tagagcgact cctttggtag
    14821 ggtgatgttt tggtcgacta aattttacca aaagtgagtc tctttttgag ttgaaacgaa
    14881 gaaaaagtgt tagtagaaaa tcgccttcga ttttccaaca acacaaaata ttatagaagc
    14941 cttttttcta aaggatttca aggtgtctgt ttgcatttat taaaaaaaag aaaaagtatt
    15001 ggcaacagaa ggcgtcacca acactagata ttatagagcc ataatatgct gtataatatc
    15061 tagtgtatag gcttaagtgg aggtatatat gaaaatatta actgttgaag atgataatat
    15121 gataagagag ggaataagtg aatatctttc agaatttgga tatactgtta ttcaagctaa
    15181 agatggaaga gaagccttgt caaaatttaa tagcgatata aatttggtta tcttagacat
    15241 tcagatacct tttataaatg gtttagaagt gttgaaagaa attagaaaga aaagcaattt
    15301 accaattcta atcttgactg catttagtga tgaagaatat aaaattgatg catttactaa
    15361 tttagtagac ggatatgtag aaaagccatt ttcattgccg gtcttaaaag ctagaataga
    15421 ttctttaatt aagaagaatt atggacattt agagaagttt gaatataagg atctatcggt
    15481 taattttaat agctatacag ctaaaataaa tggtgaagaa atagatgtaa atgcaaaaga
    15541 actagaagta ttaaaatgtt tattggataa taatggacaa gtgttaacaa gaatgcaaat
    15601 tattgattat gtatggaaag atagcgaaga aactccttat gatcgagtaa tagatgtata
    15661 tataaaagaa cttagaaaaa aattacaatt agactgtata actactataa gaaatgtagg
    15721 atataaattg gagagaaaat gaaaaaatta aagatatttc caaaaatgtt catacaaata
    15781 tt
//
LOCUS       DAHUVV010000063        15584 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.064, whole genome shotgun sequence.
ACCESSION   DAHUVV010000063 DAHUVV010000000
VERSION     DAHUVV010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.064"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            12..179
                     /locus_tag="NK767_001918"
     CDS             12..179
                     /locus_tag="NK767_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HCH6694910.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(241..2052)
                     /locus_tag="NK767_001919"
     CDS             complement(241..2052)
                     /locus_tag="NK767_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCH6694911.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2049..2414)
                     /locus_tag="NK767_001920"
     CDS             complement(2049..2414)
                     /locus_tag="NK767_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HCH6694912.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2577..2972)
                     /locus_tag="NK767_001921"
     CDS             complement(2577..2972)
                     /locus_tag="NK767_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCH6694913.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2990..3952)
                     /locus_tag="NK767_001922"
     CDS             complement(2990..3952)
                     /locus_tag="NK767_001922"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCH6694914.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3952..4164)
                     /locus_tag="NK767_001923"
     CDS             complement(3952..4164)
                     /locus_tag="NK767_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCH6694915.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4185..4883)
                     /locus_tag="NK767_001924"
     CDS             complement(4185..4883)
                     /locus_tag="NK767_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCH6694916.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4903..5445)
                     /locus_tag="NK767_001925"
     CDS             complement(4903..5445)
                     /locus_tag="NK767_001925"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCH6694917.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5577..6581)
                     /locus_tag="NK767_001926"
     CDS             complement(5577..6581)
                     /locus_tag="NK767_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCH6694918.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6578..7567)
                     /locus_tag="NK767_001927"
     CDS             complement(6578..7567)
                     /locus_tag="NK767_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCH6694919.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7564..8370)
                     /locus_tag="NK767_001928"
     CDS             complement(7564..8370)
                     /locus_tag="NK767_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6694920.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8536..9492
                     /locus_tag="NK767_001929"
     CDS             8536..9492
                     /locus_tag="NK767_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCH6694921.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9569..10087)
                     /locus_tag="NK767_001930"
     CDS             complement(9569..10087)
                     /locus_tag="NK767_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCH6694922.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10175..10324)
                     /gene="rpmG"
                     /locus_tag="NK767_001931"
     CDS             complement(10175..10324)
                     /gene="rpmG"
                     /locus_tag="NK767_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCH6694923.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10552..10998
                     /locus_tag="NK767_001932"
     CDS             10552..10998
                     /locus_tag="NK767_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCH6694924.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11193..13088)
                     /gene="asnB"
                     /locus_tag="NK767_001933"
     CDS             complement(11193..13088)
                     /gene="asnB"
                     /locus_tag="NK767_001933"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HCH6694925.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(13413..14387)
                     /gene="bsh"
                     /locus_tag="NK767_001934"
     CDS             complement(13413..14387)
                     /gene="bsh"
                     /locus_tag="NK767_001934"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCH6694926.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(14721..14808)
                     /locus_tag="NK767_001935"
     tRNA            complement(14721..14808)
                     /locus_tag="NK767_001935"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14774..14776),aa:Ser,seq:cga)
     gene            complement(14953..15519)
                     /locus_tag="NK767_001936"
     CDS             complement(14953..15519)
                     /locus_tag="NK767_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCH6694927.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
BASE COUNT         4844 a         3266 c         2621 g         4853 t
ORIGIN      
        1 aggtggattc aatgaaaaaa tttgtatctg gaatattagt aggaagtctg gcaacagccg
       61 ctgccgttgc tggtttggtt gcttctgtga aaaaaacagt gatcgatcca attgacgaaa
      121 aagaagcaat gatcgaagaa aaccgtaaaa aagcaatgcg taaacgcgtt tctcgttaat
      181 aattatttaa aaatacgcat gaagactttc taaaaaacag ataagtcatc atgcgtattt
      241 ttatttgtca gtcaatataa gttgtgctat tttatctgcc tgtctcagat tccatggatc
      301 tgtataaggt ctagctactg ctgcagttag ccaatccaca ccttcaacag gtattccata
      361 gcaataaaag tcagactgga tttcccctga agcatcaatc acgcgattcg tacctcgctc
      421 aacatctata gcttctgaat gataacttcc tgtattatct gaaaatgaaa aagggcgaac
      481 aagtttgttt tccttcaaat tttttatgac aggattactt gaacgagaaa agcttgtttg
      541 tggcaatcga gcttcaatca aatagcgact gctgatttct ttatcttgta gagaaataac
      601 atagcggttg tgcttttgct gaatatcaaa tggcggttcg atcaaatgaa aaatacccgc
      661 atctatcagt gctgctaatt ctttcgtccg aatgattggc ggaccgatcg ttaaaaagct
      721 atttagatga gtaaaccatc cccatagtat ttctttgtat tccttagcag aaaaaacgtg
      781 ccattcaata gcctgccgga ttggatcacg ccagtctttt aatgcatcaa atgttgaagc
      841 aaatgctcct gtgcaattgc ctttttccgc ttcttttatc tgcccttcaa taaatattcg
      901 gctagcttct gaaaagggct cattctgata ggcaagagga gtttctaaat acgaccaatc
      961 ccatctataa gggattagtg ctggatgctt tccgagacac tcctctgaag agagggagag
     1021 aaaatcatgc tcaaaatttt tctttggaat cggcaagtga tattcttcga tgattttctt
     1081 gtaataaaac aactcaacgt cttttttcag atagtcaaaa aatgtttcgc cagtcaaaaa
     1141 tcccgactga tgccattgct ccaggttttc ttttgttagt aaccgtggcc atgccattgc
     1201 tccgccttgc ttttgatttc ttcctcgagg gtaataaggg agcccccttc ttgatccact
     1261 gtatacaatc ggttccttgc ctgatggatg atagaccagt tttccctttt cgttctcgaa
     1321 gcgccctcct cttgatactg taaacaatcc gacataatca aaaaaactta agcctagacc
     1381 tcttaaaaag acattttctt taggcggtat cttcgaaaga tctacatctg caggattaga
     1441 aggaagctga tacagtaaaa catttttctt tgcgtagtct acccacactc tttcttcttc
     1501 tgtctcatca ttttcccagt gaccgcttgc taaaacaagc tgatcgatag aatagctttg
     1561 ctgatctgtc tgtaaaagat aaccatcttt tcttttgctt acttttttta ctaaagaata
     1621 aacaaactga atagaagctg gaaattcttc ttgcaacttt gagaaaaacc agcgttgata
     1681 gagtccgtat aaacatcgag tagattgctg atctttttcc agacgattga tttcttctat
     1741 aattagttgc ttatttttgg ttaccgtctg ttcgatataa tctgtagcag gtcctttact
     1801 ccattgaaac aagttgggtc cctgtgttac caatcctcca ctggacaatg tttcatctgt
     1861 aaaaagcgtc acttgttgag aaacagaatt cattaataac tccggtggct gattcaaccg
     1921 ccaaactcga cctccaggac cattgggatc gaaaagaaaa agttcaagag aatgtctgtc
     1981 ctgattatta tggagaatcc tttctgctac acttaatcct ctaggtccgg caccaatgat
     2041 ccctattttc atttgcatgc ctccatttcg ataaattttt tcaataaaac agcatggttc
     2101 tgatgttcat tttttgcacc ataaacaaaa gtaatgactg gatgttcttt cataagttga
     2161 ctgagtttaa aaatagcttg cttcttttct tcttcgaaaa tcaattcttg ctggtatttc
     2221 tttgcaaatg aagcaaatcg ttctggtaca tggtcaaaag ttttgcgtgc ttcattacta
     2281 ggtgcaattt ctttcaacca cagatctaac tgcgcttttt cttttgaaat tcctcgtggc
     2341 cagaatctat ccacgagtat tctatagcca tcctcttttt cagcatcaag gtaaacacgt
     2401 tttaatttga tcatagttgt ttccttcttt ctaccttttc gcttagctta tatatgtaga
     2461 taatagcaca aaataaaaaa aatgaacggc acaactatcc attgcgccgt tcatgtgatc
     2521 ttggtcaaaa tctaatgatt gattcggttt ttaaagctgt catacaatca tctgttttat
     2581 acttttttac ttttgatttt tccagaccat ccgtcatatc cacccttaag taaataaatc
     2641 tcttgataac ctgctttacg taatttattt gctgtacgga tgctcaaact tttcttttga
     2701 tcgtaaatat agacaggctg gtcttttcga atagatccta atgaatcttt caaaacagtg
     2761 taagggatgt ttcttgcacc taaaatatgc ccagcatcaa acacatcttt ttcgcgaaca
     2821 tcgattactt gtgcttttct cattccttct cgaaattctt cttctgtcaa agtcgttgct
     2881 gaacgtttgg ccattactcg tagatagact tcccaaagga tgattgcaag cacaatcaat
     2941 agcaaaatga tattaattac ccataaaacc atgtaatgcc tccgtttttt taagcaaatt
     3001 tcaaggctaa tgaggcagca ccaatgacac cagcttcatt accaagctct gctaatttga
     3061 tttttgttga ttctcttact tgcgggaaag taaattcttt aaaatatttc tctacacggc
     3121 tacgtaaaaa ttctcctgct gccgatactc ctccacctaa cacgatactt gacggattta
     3181 atgtattccc caagtttccg caagctaaac ctaagtagaa gcatacgcga tcaacaacca
     3241 tcaatgcaaa cggatcgtct tcttgagcca tttcaaagat atctttactt gttacttctt
     3301 ctccgttgtc tagcattgct ttcagttttg aatcccctgc gtattcttct gccaagaaac
     3361 gacctaggcg taccacacct gtagcgctag atacagtttc taaacatccg cgtttaccgc
     3421 aagtacattc gaacccttca ggatccacag tgatatgtcc gatttctcct ccagctccgc
     3481 tcacgccgtg aatcaaattt ccgccagcaa cgatacctcc gccaacacct gttcctagag
     3541 taacgaatac aacgtctgga tcgttttctc cagcaccttt ccatcgttca cctaaagcag
     3601 caacattcgc gtcattatcg atagcgaatt tgattcctgt tcctgcctcg atcagttttt
     3661 tcacaaattg aacttctgtc cagtttaagt tatacgcacc aataaccgtt ccagcttcgc
     3721 tgtccacgct tcctggggta cccatgccga ttccgataaa gtcttcggct ttcatattat
     3781 ataaattcaa acgatgattg attgattcaa tgatttcagg aacgatatgc ttaccatcat
     3841 ccaaaatatt ggtatcgatg ctccattttt gctgaatctc tccttctggt gttaaaattg
     3901 caaattttgc tgttgttcca cctaaatcaa tcccaataat cttcttgtcc attacaaatt
     3961 tcctttcttt cggctttctt caattcggtg ctctcttttt aatatgcgcc acgctgtcat
     4021 gtatgtatca tgatcaatca aacggctatc atacagatta cgcaattcga tagtcattaa
     4081 ttcaatgtca tagatgcgtg cacccacata gataaagatg ccaaattttt tgaacagctg
     4141 ctgtacatca tataagctct ccatctttaa gcacttcctt catattatac aagtaaacca
     4201 tattttttta aacccagtaa aatacatatt acaaaaagaa atacaaaaac tagtgtagaa
     4261 agaatccttt ggtgtatcga atagcttgtc tgttgtttcg gtagacccaa aacattccct
     4321 aacactaatc cgccaattag cccaccgaca tgtccccaaa catctacaga ggtatccaga
     4381 agaccaaaaa caaatgtcaa cacaataaat aaaagaaaat gtctgactag ctgctggatc
     4441 gctgggttat gtttaaaatg aaaccctaag ataaacaacg caccgaacaa accgaatatc
     4501 gaagtacttg caccaccaga cagaacattc gcttcgttaa aagcaaagct tgctgtattt
     4561 cccattatcc cgctgaacat ataaatcaag aaaaaacgcc aatggccata gattgcctcg
     4621 atttgctgcc ccataaaata cagtatcagt gagttcatta cgaaatgcat cagtccaaaa
     4681 tggatgaaga ttggtgtcac taaacgccac cattcattga agtgaacaag ataaggacca
     4741 tacattccgg cataataagg gatctcaagt cgcggaaata attccatcat gaggaagacc
     4801 gctgtctgta tccctaaaaa agcataggtc cataacggtc gtcttaacca tgcacgcaat
     4861 tttatttgct gttgataatt catgaaaacc tcccatttca ttttagtcaa taaacaattg
     4921 ttggatcttc tgatcaaagc tctccggttg ccattgttca ttcaattgtt cacgaaaaac
     4981 aaggctacat gtttttttat gaaaattatt taaataccgg tcgtaataac caccgccaaa
     5041 accgattcga taaccggctc tattgtagat aatacctggt acaatgagga gatcgatcat
     5101 ctccggcaaa tagagcttct ctgattgtgg ctcaagaaca ccaaaactcg aacgtacaag
     5161 aacggactgt tcatccattt cataaaacgc catctgacgt ttcggcaaag ttttaggtac
     5221 aactattttc ttttgttctt tttctgctcg ttggataacc ggctgtgtat ccagttcaaa
     5281 ttcattcgat aaagtcgtcc ctatcacttt tgctgacttc cagtactcgc tggcgaatag
     5341 ctgtttatag attcgctgtt ctttttcctt tttcttgcca cttttggcca gatttttcag
     5401 cttttcgatt cctttccggc gtaatcttac tttcaattgg tccaaaaact cacccttcct
     5461 tctctcataa aatggtatag gaaaaaaaga aaaatagcaa tcgaactttc aaataaatgc
     5521 ataaatagaa taaaaagaag acctagaaat gattggcctt ccttttttaa tgaatttcaa
     5581 ctgttcagcg cgtgtttttg tctgataaaa agtgcataca gaaaatatgg cgcaccaaaa
     5641 agggcaatca aaattcctgt aggtacttcc ccacttagta gaatcactct agcaaccata
     5701 tctgcactaa gcagaaaaat ggaaccgaac aaaccgctca gaacaaaggt ccagttattt
     5761 tctttacgta cgactagctt cgcagcatga ggagctagta gtcctacaaa agataaacta
     5821 cctgtcaccg ctacactacc gcttgccaaa gcaacagcta acaacaaaag gattttttga
     5881 cttttagcca cgttaaggcc taatccttgc gcgcgttctt gaccaaatga tagcaataca
     5941 atcctctgtg cataaagtcc tcctagcgga attaagataa ccgcccatgg aagcaatgcc
     6001 caaacataag gccagctcgc accccagatt gatcccgcta accatgcatt cacaaatcca
     6061 taattttcct tagataattg gatcgtgcat agcaatgtta atgcagaaag gccggcatta
     6121 acggcaatac cggacaataa gattcgattc atacccaaaa agccatttcg atcataggcc
     6181 gcaagataga cacagcaagc agctaaaaca ccacctagaa aggctacaac tagcaataga
     6241 aacggcagcg aatagtgcga aaagaaacct aaataaagca tcactaaaaa tcctgctccg
     6301 gcattaatgc ccaatatact agaatctgcc aattcatttc ttgttactcc ttgcaagata
     6361 taaccagaaa cgccaagaca agctcctgct aataaactga tcagtaatcg aggaactcga
     6421 aattctctaa caatcagcgc caaagaagat gattctttcc ctatgaacgt ggcaaaaagc
     6481 tgatccaggt aaataaaagg cgtaccaatc attaaactac ctatagacag gatgacaagt
     6541 acaaaaagca agaaatagaa cgtccaatat atttttttca ccgttgttcc ctccttactt
     6601 gtatcagtaa aaacggaacg ccaatcaatg caatgactaa tccaaaaggt gtttcaaaag
     6661 ggggattgat catcctcgct gtaaaatccg ccaacataac caatccaccg ccaactaaaa
     6721 aagagacagg tagaacataa cgatagtccg ttccaaccat tccgcgaaca actggcggaa
     6781 ccaacagccc aataaatgaa acaggtccga ctaacgaaac ggcaatggct gctaacactg
     6841 caactagaca gaaaacaatc aaccgcgttc gtttaggatg acttccaaga gctaatgcat
     6901 gtgtttcact caaatgaatc aaagatattg cttttcctaa tgaaaaagaa accaaaacag
     6961 caagtatata taaaggaaac aatactgcta attgtttcca tgtgatattg gcagctccgc
     7021 ctacaaacca aaaagcaaga tcctgctgca gattaaagaa ttgggtaagt gcttggctga
     7081 tagcagtaaa aaaagaacta atagctacgc cagctaaaat cagctgtaaa ggattcatgc
     7141 ccattttcga aaactgcaaa aaaccaaata cgagcaaagt tacagcaaaa gaacctgcca
     7201 atgacattag aaacacagaa aaatcagaag ctttaggcca aaaagcaaaa ctaagggcca
     7261 ttgccaacga agcacccgcg ttgattccta gtaagcctga atctgctaaa ggatttcttg
     7321 tcactccttg catcaatgca ccgctcaatg caaaacaact ccctacaaga aatgctcccg
     7381 ccacacgagg caaacgaata tttcgtatga tttggtgact ttgttttgat ctatcaaaat
     7441 caaacaacgc ctgccaaaaa tctgcttttc ccacagtcgc tgcgccattt gaaagagcaa
     7501 caaaaaaaat aatcaaaaga acaaaacaga ccaaaagcag gagtctattt ttcttcatag
     7561 tcctcataat tgatcctcct gctcttttgg caacaaatca taggttagaa aaactggttt
     7621 atttgttctt ggaagcgtga caatcgttgc atcaatctca aatatttttt tcatacactc
     7681 ttttgtgatc acttcttcag gtgtaccttc tgtgatcaac ttaccgtttt tcattgctaa
     7741 taaataatct gaaaaacgtg aagcatgatt aatgtcatga atcgacatca agatcgttgt
     7801 ttgtttcatt tgattgattt cagtcaataa ctgtaggatc tctaattggt gtgccggatc
     7861 taaaaatgtt gtcggttcat ccaagatcaa gatatctgtt tcctgagcaa gcgccatagc
     7921 gatccagaca cgctgggttt gtccaccaga cagcgttgcg atatcccgca tacgaaatcc
     7981 agctagacct gtagcatcca acgcccactc aattgcttca tgatcttgcg tgtccatgcc
     8041 actgaacagt ttttgataag ggtatcgtcc gtaagatact acttcttcta ctgtcatacc
     8101 tgtagggtgt tcactagttt gtgctaataa tgcaattttt tttgcaagtt cttttgaact
     8161 gtatgtagag atatcagagt tattgacaat gatctttcct gatttcacag gtagtatacg
     8221 ggtaaaactt ttcaataaag tagattttcc acttccattt ggcccaatca aactagtgat
     8281 tttcccttca ggaatcgtaa cagacatgtt ttcaatgatt tttttgtttt cgtagccggc
     8341 tgcaatgtca gctgctctta atctcgtcat ttcacccatc ctttcgctga aaatcattct
     8401 caattatgac gctgtttcaa catttcgtca agcgctactt tcatatctat agccaaatga
     8461 agcggttgac agcgttcgaa atatcaggaa taataaccaa tgagaaagat tctcaattat
     8521 gttaggagga aagaaatgaa attaaaaaca aaagtattca cattatctag catattgctg
     8581 ctggctggag tactgagcgc ttgctccccc tcttcagata aagaaacagc tacaactgcc
     8641 aattcgacga cttcatccaa tagtactcat acagtcacag atacacttgg acataaagta
     8701 gagattccaa acgcgcccaa acggatcatc ggcagttact tagaagacta tctgattgca
     8761 ctaggggaaa aacctatcgc ccagtggact gttggcagtg gttccattca agattatctg
     8821 caagatgatt taaaggatgt cccaacgatt tcttatgacc ttccttatga gaaagtgtta
     8881 agctttgagc ctgatttgct gttgatttct tcttccgcta ctgtcgaagg cgggaaatac
     8941 gaacaatata gcaaaatcgc gccaacttat gttgtaaaaa acggaacaga tgtcacatgg
     9001 gaagaacaac taaaagatgt cggcaaagct ttggcaaaag aaaaagaggc agaaaagatc
     9061 atttccgatt atcaaaaatc catcaagaaa actcgagaag aactagcaga taaaatcgag
     9121 aacaagacag ctgcagtgtt gtgggtgacc aacaactcag cctttatggt cagcaaaaat
     9181 cggtcaagtg gaagaatcgt atacgacgat ctggaatttg gtgttcctga tctagtggaa
     9241 gaggtctcca aaaaagccac ttctgactgg tctgctgttt cctctgaaaa gctagctgaa
     9301 ttagatgctg actacatcat ccttgtgaac agtgacaaag atgctgccat gttcaatgaa
     9361 ccaagctgga agaatcttaa agcagtgaca gacaatcatg tactgcaatt cggacctgaa
     9421 tctagttggc tatataacgg acctattgcc agtcaggaaa tggtcaatga tatcaaagaa
     9481 aaactaaatt gattttaact aaaaaaagca tcttcacgat tagtcgtgat atgctttttt
     9541 tcttattaac aaactagacg catagatttt attcttcttt tgaagcaacc ctatttttta
     9601 ctctttccca tttttgaaaa cgatgggcat atagattttc ttgatccgtt atcccctcat
     9661 actcctctat aagggcgaag tctgaaaaat caatattccc aatatacgta tcgccttcaa
     9721 acgtgctatc gatcaatgtt cgccagatcc gaccagtgta aggaagaagt tcttgaaaaa
     9781 tccgactacc acccgaaaca tagatatctt ctgagatcgt ttgactatac gacaaaagtt
     9841 catcgatcga atgcattact tccgcatttt ctttcgtgcg aaaatttggc tgcgttgtca
     9901 acacgatgat atggcgataa ggaagtgaaa gatcccccat tccttcatag gtctttcttc
     9961 ccatcactaa aattcgatcc attgtatgtt cgcgaaagaa acgcatatca tttggcaatc
    10021 gccatggtaa caaaccattt ttgccgatca gaccattttt atcttgtgcc cacatggaaa
    10081 taaacatatt atccgctcct tatgatttta gatgttttga gataaaaaaa gtctcaattc
    10141 tttgaccagc aaggaattga gaactttaaa aacattattt tgtttcacgg tgcaaagtaa
    10201 ctttgcgttc gcgtgggcaa tatttttgtt tttccaaacg atcaggattg ttacgtttgt
    10261 ttttgcttgt tagatagtta cgttctttac aagaagtaca ctctaaagtg atgtttacgc
    10321 gcatgttttt ccctcccatt tttacaagaa tcctctaaga tgactctcag acttgattat
    10381 cttaccatga ttttttctag attgctagtg tttttcacga ttttgttaag taaaactact
    10441 tgacgataaa tacaacggtt ttacttctca aacaaaatct ttttcttttt gtataaatac
    10501 ccttacactt gtttatatag attatgtttt ttaataaagg gggatactaa tatgccatgg
    10561 aacatgaagg actttcctgc atcaatgaaa aacttagata aattgacaag aaaaaaagca
    10621 atcgacatcg caaatgcatt attagatgaa gggtaccctg ataaccgtgc gatcccaatt
    10681 gccattgatc aagcaaaaaa atggtatgaa gatgctagtg aatcagaaaa acgaactttc
    10741 gaaaaagaaa aaaatccaag caagacggat gaacacgata cgaatcctcg tgctggaaaa
    10801 ctgcttgatt cagatgtgat cgtagaatat gaggaagaac aatggatcgt gaaatctaaa
    10861 ggggcaaaaa aggcaagcaa ccattttgat acaaaaaaag aagcaatcga aaaaggaaag
    10921 caagtagcac aaaacaaaga atcaactttg ttcatttata aaaaagatgg gacaaaagaa
    10981 aaagaaatcg attattgaac atatgtaaag gggttgtgac gaaaatcgtg tcacaacccc
    11041 cttatgttcg aaacaatggt gttcctctgc caactccgaa ccataagtca taatatctat
    11101 aaaacgggaa gagctgtttt ttgaaattgt ttcttatcac tcagtgttaa acggtaggag
    11161 aaaacctctt ttttattgat atttgatttt catcaaacag tcacatagtc gattgcttct
    11221 gcttgcggaa tcgtttcatc tacaaagtag actttatacc atgtcaagaa actgaagatt
    11281 gtccagattt ttctttgctc ttctttacgt ccttcgtgat ggtcatctag caacttcatg
    11341 attttttctt gatcaaaaaa ttcttttgca aaatctgctt cgaacaatgc tttgacttgt
    11401 tcgtagccgt gttcttcttt caaccaagct ttgattggca cagggaatcc taatttcaca
    11461 cgattcgacc atttttccgg aagatgtttg ttcgcagctt gtctgaaaat atcttttgtg
    11521 ttgttttgat tcaataaaaa cttacttggt atttgttgag ccaatttcat gacttcgata
    11581 tccaataaag gtactcggac ttccaaagaa cttgccatag aaagtttatc tgcctttaac
    11641 aagatatctt ttggcatcca ttgatgaaga tcgacatatt gcatcttatt gacttcatct
    11701 ttgattcctt ctgttttctc gtaatgaaca gtcatgattt catccacaga tggtgctgtt
    11761 tgatattttg gtgttaacac ttctgatgct tctgattctt cgaacactcg agcatgaccg
    11821 ataaaatatt cttttgcagg agcaagtgat tcatataaat ggatccgtcc atggaaattt
    11881 ttcatttttc cgattttacg tcctaaatta tagcgtgttc cttttggtaa ttttttcaat
    11941 ccttgagcaa tcacacggat gaattttgaa ttcgtatgga atccatacgt ttggtatccc
    12001 gcaaacagtt catccgcccc ttctccagat tggactaccc gcacactttg tgaagcaaga
    12061 tttgctaaaa agtacaaagg tacgcaagaa ggatttgaat caggttcatc taagtgatac
    12121 tggatcaaag ggaatgcttt gaacgacatg tctccgtcga tcactgctgc tgtattatct
    12181 aagtccaata gttcggttag ttttctggct tcaatcgctt cattataggt tttatcatca
    12241 aacccgattg agaaagaatg atctggtttc aatacagagg ttacataact ggaatcaacc
    12301 ccgctggata agaaagaacc aacttctaca tcactgaccg tatgtgcttc aatagaagct
    12361 tgaacggttt cgtcgatttt ttcgatccat tcttgtcggg aatgcgtttc ttttgtttca
    12421 aaatctgcat cccaatattg ctgaatgtct atttttccat ctttatacgt ataataatgg
    12481 ccttcaggca aacgatagac atctttgaag aatgtttcgc cgtttaatgg agaatattgg
    12541 aaagtcatat aaggtttcaa cgcttctttg ttcaattctt tgttaaaatc aggatgaggc
    12601 agaaaacttt tgatttctga accaaacata aatgttccgt tcatttctgc ataataatat
    12661 ggtttgatcc caaagtggtc tcttgcaccg aataactcat tcttttcatt atcccaaata
    12721 gcaaaagcaa acattccgcg gatcttttgc aataattctg ttccccattc ttcgtaacca
    12781 tgcaaaagga cttctgtatc tgcatgtgtt tgaaaaacgt ggccagctgc aatcaattct
    12841 tctctcaaag gctggtaatt atagatttcg ccattaaaaa taatgatctt tgtccgatct
    12901 tcattgtaaa taggctgagt accgccctct aagtcaataa tacttaatct tctgaatcct
    12961 aaagcaacat gtttatcgat atattctcct gaactattag gtccacggtg aacgattcga
    13021 tccatcattg tattaataat tgtttttttg ttgtccttgt cgttaacaaa acctacaata
    13081 ccgcacattt aactttcaca tcctaatata aaattactgt ttatttcatt catatgcatc
    13141 ttatcataga ataaatagcg gacgaatatt ttctctctta caatttcgtc acgtgaaaac
    13201 aaaagatgaa agtttctgtt tttacagaaa ataaagtccc aatatctaat ttaccatgtt
    13261 ttcttttttg aggctacctt attctcttcg tgttaaaaaa atattagtgg atgctatttc
    13321 caaaaaaacc agcaaaaaaa gacacgacct aaagatcgtg tctaattatg actgtatcag
    13381 aaaatatgat accaaacgga tctattcatt aattaattga catagtttat ctgttgctct
    13441 tttatgactg gataactgac caattcatga cgatccaagt cttctttatt catatttaca
    13501 gcagtaattt gactgtcttc atacgtacga taataataaa tccctttatc aacgttacag
    13561 caagaagaat aaatcgtata ttcgtatttt ctttctccag tatcgcatac tcctttttgt
    13621 tgctcgactg agcctagtat atgaaaaaat tgactgatac tttcagattc tgaatcacct
    13681 gatgcagcat tcattttagt aaatgtggct tttacaaatc gagaaacaga agagagatct
    13741 ccaggtaagc ccaatcctcc catcccacga ctataaacat tcaagctgat ctggtttgaa
    13801 aaatgatttt ctggtgttcc atttgatagc gagcgataat tgttcagatt aaataattga
    13861 taatcaaatg gcggattatt tgtcaacaca ccgacaggat tatcatataa atgaagtcca
    13921 tctttcatac tttcaattac tatcgatttt tctttatcag ctaataacca gtgtaaaggt
    13981 gacaagggta gtttttcact aaaatcgata ttgactagat tgatagtagc caataattct
    14041 ttagcttcgt ctatggttga gcattgccct agtatccatg gaataaactc aaatggtgca
    14101 acgttatctt ttccttcttg taattcttta taatcagcat tgccaggaaa attcagtcct
    14161 gccatactca accctttttc atttgtagcc tcataataga gtggatagtt ggatacccca
    14221 gctgctattc ctatcattgc ataatgttta tctaaatttt ttacctttct aaaatcgaaa
    14281 cgataatttc ttggagtaat agtgaccact tcattataag acatctcata atcaaaattc
    14341 cttccaaaat aatggtcttt cgttgcataa gtgattgcgg tacacatcgt ttcccgtcct
    14401 aaaacaaatc gtttttacac ttttatctta catctagtat tttttttatc aaaaaaaggc
    14461 caaagaagat tgatttacta gcatttataa aaaatagtac tcagggggaa tcaaaaaata
    14521 ttgtaggtta aaagtacaac aataaggggc tgtgacaaaa gtcttgtcac agccccttat
    14581 tccgaacaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaatc aaacgaaaaa
    14641 ataggaaaag ctatttttgt ttggccgttc ttattacttg atgcaggaca gcttcttcac
    14701 aacctctaat cgttgatata aggaaggtac aggatttgaa cctgcgcgcc ggtattagcc
    14761 ggttcgccgg atttcgagtc cggtgcatta ccactctgcc aaccttccgt aagtactatt
    14821 tttaaacttt tttcacgaaa tgtcaagcct atttctaata gataaataac agaaatcgct
    14881 ttttttagca aaaaagtcca ttgacagttt gcagccaacg gacttaccga tcaaattcat
    14941 actgatagaa cactatctga ttatttcagg attatctaat gctaaaatcg agtcaattgt
    15001 ccgtttgatc agtgcaatga tttgttcttc tgttggatgc atgatgtttg atgacatcaa
    15061 tgacgctaag ccagtcactg ctacccatgt accagtatgc agagcatcga catattcatc
    15121 cgataattga ttgtatcttt catcttcttt aactatttca cggaaataat tgtatgaaaa
    15181 atcatgcatt ttctttccgt ccccatgttc atcaatgtac aatgccatgt aaagactatg
    15241 atgctttttt gcaaagtaga tatagttcaa accgagatca atgattttat caccagtacg
    15301 ttcaataggg aaaacatctt cttttagttg ttcaaagata gaatccagca acgtattttt
    15361 taaatcttcc atgtttttga attccagata aatcggctgt gtcgaaatcc ccatcttttt
    15421 agcaatattt ctcgcagtaa atcctttaaa cccttccttt gcaacaacat cataagcagc
    15481 ttttagaatt tgatccttgg tatagacttt tcttctcatt aaaactcccc cacttcttta
    15541 ctgcagtata tgttgtttta ttgtacccta tttttagcat tttt
//
LOCUS       DAHUVV010000064        15286 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.065, whole genome shotgun sequence.
ACCESSION   DAHUVV010000064 DAHUVV010000000
VERSION     DAHUVV010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.065"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="NK767_001937"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="NK767_001937"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="NK767_001938"
     CDS             complement(580..1353)
                     /locus_tag="NK767_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCH6694928.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="NK767_001939"
     CDS             1497..1847
                     /locus_tag="NK767_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCH6694929.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="NK767_001940"
     CDS             1828..2544
                     /locus_tag="NK767_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6694930.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="NK767_001941"
     CDS             2544..3992
                     /locus_tag="NK767_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCH6694931.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="NK767_001942"
     CDS             complement(4062..4571)
                     /locus_tag="NK767_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694932.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="NK767_001943"
     CDS             complement(4561..5286)
                     /locus_tag="NK767_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694933.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5509..7629)
                     /locus_tag="NK767_001944"
     CDS             complement(5509..7629)
                     /locus_tag="NK767_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCH6694934.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            7859..9037
                     /locus_tag="NK767_001945"
     CDS             7859..9037
                     /locus_tag="NK767_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCH6694935.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9053..9811
                     /gene="aroD"
                     /locus_tag="NK767_001946"
     CDS             9053..9811
                     /gene="aroD"
                     /locus_tag="NK767_001946"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCH6694936.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            9834..10319
                     /gene="ybaK"
                     /locus_tag="NK767_001947"
     CDS             9834..10319
                     /gene="ybaK"
                     /locus_tag="NK767_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCH6694937.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10390..11643)
                     /locus_tag="NK767_001948"
     CDS             complement(10390..11643)
                     /locus_tag="NK767_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCH6694938.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            11760..13109
                     /gene="gdhA"
                     /locus_tag="NK767_001949"
     CDS             11760..13109
                     /gene="gdhA"
                     /locus_tag="NK767_001949"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCH6694939.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13221..14567
                     /locus_tag="NK767_001950"
     CDS             13221..14567
                     /locus_tag="NK767_001950"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCH6694940.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(14875..15261)
                     /locus_tag="NK767_001951"
     CDS             complement(14875..15261)
                     /locus_tag="NK767_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCH6694941.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         4990 a         2737 c         3104 g         4455 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
     5521 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
     5581 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
     5641 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
     5701 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
     5761 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
     5821 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
     5881 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
     5941 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
     6001 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
     6061 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
     6121 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
     6181 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
     6241 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
     6301 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
     6361 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     6421 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     6481 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     6541 gaatgtacca gcgttcccat ggaataccgc actaatgtaa ccttgtgttt gacttaaaat
     6601 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     6661 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     6721 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     6781 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     6841 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     6901 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     6961 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     7021 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     7081 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     7141 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     7201 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     7261 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     7321 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     7381 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     7441 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     7501 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     7561 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     7621 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     7681 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     7741 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     7801 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     7861 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     7921 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     7981 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     8041 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     8101 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     8161 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     8221 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     8281 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     8341 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     8401 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     8461 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     8521 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     8581 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     8641 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     8701 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     8761 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     8821 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     8881 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     8941 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     9001 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     9061 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     9121 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     9181 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     9241 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     9301 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     9361 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     9421 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     9481 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     9541 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     9601 ggagttgact agacgggcaa atgctttttt ctccatgcct attattacga tgtccatggg
     9661 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     9721 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     9781 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     9841 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     9901 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     9961 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
    10021 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
    10081 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
    10141 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
    10201 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
    10261 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
    10321 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
    10381 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
    10441 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
    10501 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
    10561 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
    10621 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
    10681 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
    10741 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
    10801 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
    10861 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
    10921 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
    10981 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
    11041 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
    11101 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
    11161 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
    11221 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
    11281 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
    11341 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
    11401 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
    11461 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
    11521 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
    11581 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
    11641 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
    11701 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
    11761 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
    11821 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
    11881 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
    11941 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
    12001 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
    12061 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
    12121 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
    12181 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
    12241 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
    12301 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
    12361 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
    12421 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt gtttccggaa
    12481 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
    12541 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
    12601 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
    12661 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
    12721 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
    12781 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
    12841 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
    12901 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
    12961 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
    13021 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
    13081 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
    13141 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
    13201 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
    13261 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
    13321 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
    13381 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
    13441 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
    13501 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
    13561 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
    13621 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
    13681 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
    13741 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
    13801 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
    13861 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
    13921 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
    13981 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
    14041 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
    14101 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
    14161 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
    14221 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
    14281 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
    14341 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
    14401 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
    14461 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
    14521 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
    14581 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
    14641 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
    14701 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
    14761 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
    14821 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
    14881 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
    14941 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
    15001 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
    15061 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
    15121 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
    15181 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
    15241 caaccctaaa atttttttca tgaaagatct ccttttttat ttttat
//
LOCUS       DAHUVV010000065        15237 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.066, whole genome shotgun sequence.
ACCESSION   DAHUVV010000065 DAHUVV010000000
VERSION     DAHUVV010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.066"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(21..251)
                     /locus_tag="NK767_001952"
     CDS             complement(21..251)
                     /locus_tag="NK767_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694942.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="NK767_001953"
     CDS             complement(687..1253)
                     /locus_tag="NK767_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCH6694943.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="NK767_001954"
     CDS             complement(1253..2209)
                     /locus_tag="NK767_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCH6694944.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="NK767_001955"
     CDS             complement(2209..2865)
                     /locus_tag="NK767_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCH6694945.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="NK767_001956"
     CDS             3235..5253
                     /locus_tag="NK767_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCH6694946.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5323..5529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5680..8094
                     /locus_tag="NK767_001957"
     CDS             5680..8094
                     /locus_tag="NK767_001957"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCH6694947.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="NK767_001958"
     CDS             8442..9995
                     /locus_tag="NK767_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCH6694948.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="NK767_001959"
     CDS             complement(10376..12160)
                     /locus_tag="NK767_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6694949.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="NK767_001960"
     CDS             complement(12175..13896)
                     /locus_tag="NK767_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6694950.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="NK767_001961"
     CDS             13996..14757
                     /locus_tag="NK767_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCH6694951.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4957 a         2792 c         2996 g         4492 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcatt
//
LOCUS       DAHUVV010000066        15220 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.067, whole genome shotgun sequence.
ACCESSION   DAHUVV010000066 DAHUVV010000000
VERSION     DAHUVV010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.067"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(28..726)
                     /locus_tag="NK767_001962"
     CDS             complement(28..726)
                     /locus_tag="NK767_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCH6694952.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="NK767_001963"
     CDS             complement(719..2071)
                     /locus_tag="NK767_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HCH6694953.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="NK767_001964"
     CDS             complement(2294..3694)
                     /locus_tag="NK767_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCH6694954.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="NK767_001965"
     CDS             3855..4592
                     /locus_tag="NK767_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCH6694955.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="NK767_001966"
     CDS             4717..5196
                     /locus_tag="NK767_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HCH6694956.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="NK767_001967"
     CDS             5193..5960
                     /locus_tag="NK767_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694957.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..6759
                     /gene="lepB"
                     /locus_tag="NK767_001968"
     CDS             6190..6759
                     /gene="lepB"
                     /locus_tag="NK767_001968"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCH6694958.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6863..7465)
                     /locus_tag="NK767_001969"
     CDS             complement(6863..7465)
                     /locus_tag="NK767_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCH6694959.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7571..8040
                     /locus_tag="NK767_001970"
                     /pseudo
     CDS             7571..8040
                     /locus_tag="NK767_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8146..8730
                     /locus_tag="NK767_001971"
     CDS             8146..8730
                     /locus_tag="NK767_001971"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCH6694960.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8733..9806
                     /gene="prfA"
                     /locus_tag="NK767_001972"
     CDS             8733..9806
                     /gene="prfA"
                     /locus_tag="NK767_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCH6694961.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9799..10638
                     /gene="prmC"
                     /locus_tag="NK767_001973"
     CDS             9799..10638
                     /gene="prmC"
                     /locus_tag="NK767_001973"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCH6694962.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10631..10810
                     /locus_tag="NK767_001974"
     CDS             10631..10810
                     /locus_tag="NK767_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694963.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10832..11857
                     /locus_tag="NK767_001975"
     CDS             10832..11857
                     /locus_tag="NK767_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCH6694964.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12032..13276
                     /locus_tag="NK767_001976"
     CDS             12032..13276
                     /locus_tag="NK767_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCH6694965.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13429..14376
                     /locus_tag="NK767_001977"
     CDS             13429..14376
                     /locus_tag="NK767_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCH6694966.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14510..15139
                     /gene="upp"
                     /locus_tag="NK767_001978"
     CDS             14510..15139
                     /gene="upp"
                     /locus_tag="NK767_001978"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCH6694967.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
BASE COUNT         5188 a         2720 c         3112 g         4200 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
     6541 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
     6601 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
     6661 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
     6721 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
     6781 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
     6841 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
     6901 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
     6961 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
     7021 gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa
     7081 tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata
     7141 gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc
     7201 ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg
     7261 aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag
     7321 ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta
     7381 gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga
     7441 tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa
     7501 cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc
     7561 tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg
     7621 agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat
     7681 cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt
     7741 tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata
     7801 aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac
     7861 gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata
     7921 tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca
     7981 aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga
     8041 gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa
     8101 atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt
     8161 tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat
     8221 tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt
     8281 ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa
     8341 acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat
     8401 gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg
     8461 gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga
     8521 tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc
     8581 tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc
     8641 gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt
     8701 cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag
     8761 accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc
     8821 gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc
     8881 gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg
     8941 atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag
     9001 tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata
     9061 tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc
     9121 ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag
     9181 ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg
     9241 tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg
     9301 aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag
     9361 aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg
     9421 ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag
     9481 ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga
     9541 aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg
     9601 ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt
     9661 tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga
     9721 ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa
     9781 aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct
     9841 tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga
     9901 aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa
     9961 cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg
    10021 ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct
    10081 cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta
    10141 gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga
    10201 aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa
    10261 aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta
    10321 atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg
    10381 gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac
    10441 gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat
    10501 ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag
    10561 caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg
    10621 atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa
    10681 aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact
    10741 attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa
    10801 gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga
    10861 attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac
    10921 agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt
    10981 aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa
    11041 agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg
    11101 accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg
    11161 attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact
    11221 ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac
    11281 tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc
    11341 tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat
    11401 gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt
    11461 tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata
    11521 caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc
    11581 agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc
    11641 ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc
    11701 ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga
    11761 tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt
    11821 gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct
    11881 tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta
    11941 actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa
    12001 attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga
    12061 tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc
    12121 ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa
    12181 atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt
    12241 tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca
    12301 gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga
    12361 cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa
    12421 cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat
    12481 tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg
    12541 tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt
    12601 aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca
    12661 tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg
    12721 ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc
    12781 ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc
    12841 attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc
    12901 aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac
    12961 agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga
    13021 gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact
    13081 aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt
    13141 caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga
    13201 tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc
    13261 gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct
    13321 tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
    13381 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
    13441 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
    13501 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
    13561 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
    13621 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
    13681 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
    13741 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
    13801 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
    13861 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
    13921 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
    13981 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
    14041 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
    14101 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
    14161 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
    14221 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
    14281 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
    14341 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
    14401 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
    14461 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
    14521 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
    14581 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
    14641 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
    14701 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
    14761 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
    14821 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
    14881 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
    14941 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
    15001 tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
    15061 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
    15121 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
    15181 ttttttggtc atttttaacg tttgatgcag aacagctttt
//
LOCUS       DAHUVV010000067        14255 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.068, whole genome shotgun sequence.
ACCESSION   DAHUVV010000067 DAHUVV010000000
VERSION     DAHUVV010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.068"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(421..1605)
                     /locus_tag="NK767_001979"
     CDS             complement(421..1605)
                     /locus_tag="NK767_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCH6694968.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1630..2637)
                     /locus_tag="NK767_001980"
     CDS             complement(1630..2637)
                     /locus_tag="NK767_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCH6694969.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2809..3168)
                     /locus_tag="NK767_001981"
     CDS             complement(2809..3168)
                     /locus_tag="NK767_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694970.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(3140..3511)
                     /locus_tag="NK767_001982"
     CDS             complement(3140..3511)
                     /locus_tag="NK767_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HCH6694971.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3528..3830)
                     /locus_tag="NK767_001983"
     CDS             complement(3528..3830)
                     /locus_tag="NK767_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6694972.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(3847..4317)
                     /locus_tag="NK767_001984"
     CDS             complement(3847..4317)
                     /locus_tag="NK767_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCH6694973.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(4292..4570)
                     /locus_tag="NK767_001985"
     CDS             complement(4292..4570)
                     /locus_tag="NK767_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCH6694974.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4567..5616)
                     /locus_tag="NK767_001986"
     CDS             complement(4567..5616)
                     /locus_tag="NK767_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HCH6694975.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5573..6502)
                     /gene="tadA"
                     /locus_tag="NK767_001987"
     CDS             complement(5573..6502)
                     /gene="tadA"
                     /locus_tag="NK767_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCH6694976.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6634..8388
                     /gene="ade"
                     /locus_tag="NK767_001988"
     CDS             6634..8388
                     /gene="ade"
                     /locus_tag="NK767_001988"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCH6694977.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8486..9148)
                     /gene="trmB"
                     /locus_tag="NK767_001989"
     CDS             complement(8486..9148)
                     /gene="trmB"
                     /locus_tag="NK767_001989"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCH6694978.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(9218..10000)
                     /locus_tag="NK767_001990"
     CDS             complement(9218..10000)
                     /locus_tag="NK767_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCH6694979.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(10204..11415)
                     /locus_tag="NK767_001991"
     CDS             complement(10204..11415)
                     /locus_tag="NK767_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6694980.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11408..12145)
                     /locus_tag="NK767_001992"
     CDS             complement(11408..12145)
                     /locus_tag="NK767_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6694981.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            12283..12717
                     /locus_tag="NK767_001993"
     CDS             12283..12717
                     /locus_tag="NK767_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCH6694982.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12710..13054
                     /locus_tag="NK767_001994"
     CDS             12710..13054
                     /locus_tag="NK767_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCH6694983.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            13234..14244
                     /locus_tag="NK767_001995"
     CDS             13234..14244
                     /locus_tag="NK767_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCH6694984.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         4540 a         2907 c         2401 g         4407 t
ORIGIN      
        1 aaaaaacttg attttcatac ctgtgctttc acgattcagg caaagtacag gtggaaaatc
       61 aagtttttat tattcgatca atgaatgttt cggatcggtt ttctagctaa acataaagcg
      121 aagaataaag tcgacagccc acgaggctga tctaaaaatg ttttttcttt ttacatatgt
      181 aatgtccctt ataaagatga atgaaaataa aatattggga ttttcctgaa aatcaataaa
      241 taaaatgcca gtcttttccc ttttgaccac ttttccaagc aaaattccct aagaagatcc
      301 tgtaaaaaag aagggaaatt gaagttaaat taccattttg cggtcttctc cgcaaaaaaa
      361 acacatgcaa gaattccctc ttgcatgcgt ttcatctttt atcctaacga atgctttggc
      421 ttagccgcgc agacgctcca catcacgggc aatcattagt tcctcatctg ttgggatcac
      481 taatactctt acttttgatt cttttgtaga gatatctact tcttcaccac gcaattcatt
      541 caacgctgga tcgatttcac aaccaaacca agacatacct ttgatgattt cactacgtac
      601 atgtccgtca ttctcgccga taccagcagt aaagacaatt gcatcaacac cattcatcgt
      661 tgtcacataa ccgccgatgt attttcggat gcggtccacg aaaatgtcat aagctaaacg
      721 aatatgttct ttttcatagt tcttttcaag atcacgcata tcactagaca atcctgttag
      781 gcctagcaga ccagatttct tgttcaaaac attgatcatt tcatcaatat caatctctaa
      841 tttttccatt aaatatggga gaacagctgg atcgatatca cctgaacgtg tacccattgt
      901 caccccagct aatggtgtga atcccataga agtatccatg gatttacctc catcgactgc
      961 tgtgatcgat acaccatttc ctaagtggca agtgatgatt tttagttctt cgatcggttt
     1021 gttcaataat tttgccgcac gttccgacac ataacgatga cttgtgccat ggaagccata
     1081 ttttcttact ttgaaatctt cataataaga caccggtaag ctatataaat aattttgctt
     1141 tggcattgtt gagtggaaag atgtatcgaa tacagccaca ctcaaaatgt caggtaaaat
     1201 ctttttaaac gctttgatcc ccattaagtt tgcaggatta tgcaaaggag caaattctgc
     1261 taattcagca atgcggtcca ttactttctc atcgatcact actgaatccc cataagtttc
     1321 tcctccgtga acaacacggt gccctactcc ggtgatttca tcataagagc ttaaaatttg
     1381 cagttcaatc aactgatcta acagcatttt tactgctact tcgtggtttt cgatatccat
     1441 gatttgttcg aatttttgat tatcgccgta cttgatggta aaaatagaat cttgtaaacc
     1501 gatacgttcg acgatccctt tcgcaactac ggtttcttct ggcatttgat ataattgcca
     1561 tttcaaactt gaactcccag cattgattgc aattgtttta gacatatgac tctccttctc
     1621 gttgaatgat tacagagcag atgatttcca gtttttaaac tcgttgaaaa attccgtcac
     1681 cttctctggt tcttttaatg aagcaagttt cacaagcaag acttctttta cttgttgtgc
     1741 ttgtggtcct tttttctgta agatcaaaat acttttttgc gattgtttat tacggaacaa
     1801 ttcatctggc aattgaatca ttccttgaag ataaccttct tctttaaacc attttttgat
     1861 ttcttcactt tgatccgttt ctaaaaatcc actcggcatc aaaaacaaac caaaaccatc
     1921 cggcttcaca tatttcattg attgctctag caataaatga tgtgcataac tatggccttc
     1981 ttcggtactt gttaaaaact cttttgcttt ttcgtcattc ggataataac cgattggcaa
     2041 atcgctgatt gcaaaatcga caggatctat taataattcc tgcaacccgt cttgatgaaa
     2101 gtattgaaca tttgcttgtg tcaaatcact tgtagaagct gcgacagcca acaatgtatc
     2161 atcaatatcc acaccaattc cttctgtttg gtaccccgca ttcgataggt ttaaaagaac
     2221 tgttaataac aagttgccca ttcctgccgc aatatccaga atcttaaccg gtgctttttt
     2281 atttgtgtat agttgttcaa taagaaaaac aaataaaaaa ccgatactat caggagtgag
     2341 ttgatggttc gcctgcaaat gttctgtctg gctgcccttc aataaaagta attgagacag
     2401 tctgcgccat tcttctggct cgaaagaaag tttcttcaac tcttcataaa gagctgtgat
     2461 ccgttgcgtt gtctcttttg tcggcactcc atccaccaca cgtacttgat aatcatctat
     2521 taaattttct gcattttcga cataggcttc taaaaaggaa gttcccaatg cattctgtaa
     2581 aagctgaata gcttccaggt tttgattaaa cccttgttcc attttttctg gtaacatggt
     2641 gcacctcatt tctagatata ttataacaat tttttcacct gtttaattct atcgaacaat
     2701 tgacaaaatt tcaatcgttt tcactgtttt ttatttgtga aacggcaaac atttttttat
     2761 taaaaacagt gttataacag aaaagagggc gttttcacga acattccatc attctgtact
     2821 agtatctgta tcatttttta cttcttctgt gaaccgaaag ataaacggtg agacttccac
     2881 ctctaagaat agggactgtt gtacccactg atatgttaca gatccagttg tgtagtaaaa
     2941 aactccttcc gaactttcgg caagcgtaga ctgttccgat aaagccatct gcttcattat
     3001 ttttgctata taataatttt tgattcgtat attaagatcc attgacaaac ggtagctttc
     3061 taatgctgcc atcaataaaa acgtacataa aagaaataaa agcaagacag aaaataaaat
     3121 agagccctta taacattttc tactgatcgt tttttgtcca tatggcatat ccatcttctc
     3181 cattttcaaa gtgtacataa aaagaaattg tgtgttcttt ttccaataat tgaagctcag
     3241 tgatacttgt taacataggt tgatgtccgc cggaagttct tcttttacgt atcattgcct
     3301 gatatctttc gatgatatac tgtttatcct cgatcgtgta gtgcagttgc tgatttttca
     3361 cagagacatt gtgactgctc ttgagttctt cttctaattg tgctaaaaat attagccact
     3421 ctttttgatc tttacgctgc aaaaattcac ttacttggat ggtttgctga atcaaaagat
     3481 tgaatgtgag caatagactg ctcaatatca tcagtccaat tagacattca atcagcgtaa
     3541 atgctggaat tacttgtttt ttcaatactg atcgcttctt tctccatatt gacaaacact
     3601 ttttcgatta ctccattatt atcgacaatt tggatatcgt atgggatcat ttcttccctt
     3661 cgctctggtc ttttgtgata catccgctgc gtcttgatat cttcataaat cattcgaata
     3721 aatagcagtt cctccccact tttcttttct gcctgaagca aaaacagctg gccaaagctc
     3781 actaagtaga cacagacaga agcgatcata agcgctgtaa gggactccaa caaaacaaat
     3841 cctgttttat tcaattcttt ttgtatagcg accactccct atctggaatt gatacgtaat
     3901 cgtccgctct ttttcttccc aataaaattg ataagctttt agtgaacttt cattcccggt
     3961 tctagcagca aaagtaatag aagcatggcg tttcaaggta atctcatcag gaatatctaa
     4021 tacttcccaa tttattttat ctggattcgg aacatgaaaa atgatttgat tctcttcact
     4081 attccaataa aagcctgttt ggatctgatt cacaatagcg attttttgcg tagaatagat
     4141 tcgtttttca aattggccga aaaattgata gactgccaat tcattcttcc atgcagaaaa
     4201 tgcgagaact ggaaaagaaa ggatcgttgt aagtaataaa atcagtaaga gtgattcaaa
     4261 caacgtatat cctgatgcta aaaccttacc ttcaaggccg ctgatagatt tctgcatgtt
     4321 ctcgtttgat atagccttca ttcaacaatt cctccacagt aggaactctt ccattatttt
     4381 gaagcgcata gagctctttt tgtgtgtcta ccaactggat gatcgcttca cgactttctt
     4441 ggtctacatg attcctataa cgcgacaaat tagggacgaa caacaacact aatacactga
     4501 tgatcagtaa gacgattagc atttcgatca atgtaaaacc gctataataa gatatttttc
     4561 tctttttcat ggcaaaacct cctctatgct tccgtaaaca ggtaataaaa cagcggcata
     4621 aactaataaa atcaaagccg ctacgcccaa aaaaatgatt ggctgaataa aatgcatcca
     4681 gtgatgaaga cgtttgaaaa gctgttttct tgtccattcg ctgtaaaaaa tcagttcttt
     4741 acctaaactt ccctttgcct ctccctgtaa aacaatttta ctgaattctt ctgatagaaa
     4801 acgataattc tggaactgct cagccaatga catacctgcc tccaatcctt gaatcaactt
     4861 agaggcaaga tgctgaatca gactttcttg tttcgtttcc tttaagcaga ctatgatttg
     4921 ccttaattcg aaaccttcat gaaaaagctt tccaatctct agtgacaaat aagccgattg
     4981 atacaaggaa tatatcggtc caatcaataa ttgtctgctt atccattcac tctgatcaac
     5041 aggagacaac ttcatcagac gataccttac tagtaaagcc aatagagtaa ccgctaggat
     5101 gaatagtaga acataccaat gaaagacttg tatcagctga ataccccaat gctcttgcat
     5161 gaccattcct gaaccaataa gctgaggcaa aacgaacaac cgcatactaa acaagatacc
     5221 tagcaaaaag gtgagcaata gcatcggata tgcaagcagc tttttcaaat cattgcgaaa
     5281 tccttcaaca agccgaaact gtttggcaat ttccaacaaa gttgtcggca gattcccatg
     5341 acgttcagct aactcgatct gtaccagttg ctctgctcca tatcctattt gcgaaagtac
     5401 ctcactaaac gcttgccctt cctttagctg atgttggata tttgccaaaa taacaggtgg
     5461 aaacgaacgg atcgtgagta ataaataaag ggattcttgc agactaaaac cagataaaag
     5521 cagttctccc atggtttgtg caaactggtt ttgctggctt cttgttaatc ctttagaata
     5581 gttctgactg ccaaatattt tcctgagctt cttcataact ttcctcccat gattttttta
     5641 ctgattcttc ctccataaat tgataactcc ataaaacaga aggatggtga ttggcatctt
     5701 ccaaaagttc ttgataaatg acaccagcta aacattcaga tagctcttct tttttctttg
     5761 tcagttcatg gattctgacc aatgtacctt caagattgcg tgcatgtacg gttgcaaaaa
     5821 cccggtgccc tgttagtgct gctcttaccg cagcatgtgc agttgattca tctcttatct
     5881 cgccaatgat tagcaagtct ggacgatgcc gtaatgcgag tttcaaaagt gtatcataag
     5941 tctgtgctat tttcggattg atctgtagct gcagaaaaaa cggttcttct atctctacag
     6001 gatcttcgat agtaatcact tgcaattttt cttccaaagc caaccgatac attagagaag
     6061 tcttgcctga tccgactggt ccgctgaaaa ggtataatcc acgtctgacg gttttctgta
     6121 aaatagttgc aaaatctcga gaactaaagc aacgatgggg tgtttcaaaa gtaccgtata
     6181 atagccgaat cactaaactc tctcgttgaa ggtaatctcc tacagaagac aaccgcagac
     6241 gaacttcctg tttttctaaa gaataagtaa atgcacctaa ctgagctttt cttttttctc
     6301 cgatatccat ctgtcccaaa tacttaaatc ttgagatcag ttgctgtcca tctgccgaag
     6361 agactttctt ccagaatgtt cgtttttttc catagcgaaa atagatgtaa aatacttctg
     6421 aagctggcaa tatatatacg tcttgggcat gtttggcaaa actttcactt atcatttgat
     6481 ccgataatcg ttttatttcc attattctcc cctccacaaa aaacatacgt ctttttgatc
     6541 tttttgtttc aaataaaatg aatttacaaa aaaatattgg caaaagccga atttatgtta
     6601 tacttataaa aatttatctt gggaggcact ccattggaaa caacattaag gaaatatatc
     6661 gaagtagcaa gtaaaaaaaa gcctgctgac ctagtgatca agcatgctaa gatcgtcaac
     6721 gtctttacga aagaaatcat ggaaaaagat gtagcaatct gcgaaggaat gatcgtagga
     6781 atcggtgaat acgaaggaaa aagcgtacat aatgctaatg ggcaatatct agtgccagga
     6841 ttcatcgatg gccatgtgca tatcgaaagc tcacttttat caccaagcga attttcaaaa
     6901 gtatccttgc ttcatggagt tactactgta gtgacagatc cccatgaaat aggaaacatt
     6961 gctgggagta caggtcttga gtttatgata gaagatgcaa gacaaacact catgaatatc
     7021 ttttttatgc taccttcttg tgtacctgta accccttttg agaccaatgg tgcaaattta
     7081 aatgcaaaaa aattagcccc ttttttacaa aaacccgaag ttttaggctt agctgaagtc
     7141 atgaactatc aagcagtagc aaataatgaa acagacatcc tagaaaagat ccaattgatg
     7201 caccagcata agaaaaaaat tgacgggcat gcagctggta tcggcatgga agagttaaat
     7261 gtttatcctg cagcaggtat tcggacggat catgaagcta caactgcaaa agaagctaaa
     7321 gaacgtttgg atcttggtat gtatttaatg gttcgagaag ggaccgttgc aaaagatctg
     7381 gcatcgctac taccggcgat cacacctgaa aattcaagaa gatgcttctt tgtcaccgat
     7441 gataaactga tcaatgattt agtaaaagaa ggatcaatcg atcatatcgt ccgtcaagct
     7501 attgcacttg gaatcgatcc attacaagcc attcagatgg caacattgaa tgcagcagaa
     7561 tgttttgatt tgaaaacact aggtgcgatt gcccctgggt atcaagctga tttcttctta
     7621 acagacgatc ttaccaagct gccaattcat gaagttttct ataagggaac attagttgtt
     7681 caatctgggc aactccaaga ccatttattt gctgatcatt caaattcata tacgtgttgt
     7741 ctgccaaaac taaacgctca accattaaat ccacattcgt tagagctgct tctttcttct
     7801 tcacaagccc atgtgatcga aattatcccg aatagtctag tgactaaaga tttggtagaa
     7861 aaagtggacc gacaaggcga acgattcatt ccttcaatcg aaaaagacca attaaagatt
     7921 gcggtgatcg agcgtcacca tcaaacggga aacattggat taggcatagt gaaaggattt
     7981 cagctgaaac aaggagcgat agcgacaacg attgcccatg actcgcataa tctagttgtc
     8041 gttgggacaa acgacgagga tatgctgtat gctgctaatc ttttgattca aaaaggtggc
     8101 gggatgaccg ttgtcaacaa acagcaggaa atagcttgtg tttctcttcc tataggcgga
     8161 atcatgacac aggaaccttt ttcgatcgtc tatgaacagt tgaatgaatt aataactaaa
     8221 gcacatcaac taggagcagc taaaaccttt gatccttttt tgacactttc tttcttaaca
     8281 ttatctgtca tacccgaatt gaagatcacc gacaaaggat tattttcatt ttctcgcttc
     8341 caattgattc caccttgtgg aaaaaccgat gaaataaata cattttaaat caaataaatc
     8401 ccagaaaata cattacatct gtaatgttca ttttctgaga tttatttatg gatattaatg
     8461 tttttatgaa agcagaaagt tagtttcaca gacttttctt tttggtctta tcttgaaatt
     8521 gagcttctac tcgataaata cgttgacctt ttgaagaaaa tttttcttca tactccgtca
     8581 taatattgcc ttcaaattga ctttgatgta aatctaacca tacctgtttg aggatcatgc
     8641 catattgaga aaaacttgcc agagaatact caaacaatcc ttgattatct gttttgaaat
     8701 ggatttctcc atttggacga agaatctgtt catctacagc aagaaaagtc ttatatgtca
     8761 atcgccgttt ttcatgacgt ttttttggcc atggatcgga aaaattcaga tagatttgat
     8821 ctacttcgct gtcagcgaaa tactctgtca acgctgatcc atccacatgt agaagtttca
     8881 ggttaggaag cggttgttcg atcaacttgt ctagtgcaat cgaaacaaca ctgacctgca
     8941 tttctactcc aatataattg atttcaggat gggcttttgc cattccagtg atgaactggc
     9001 ctttccccat accaatctca atgtggattg gatgatcgtt ttcaaacaat tcattccatt
     9061 ttcccttcca caatgttgga tcactaatta caaaattcgg gtgagccgct aacatttccg
     9121 ctgctcccgg acgatttcgg acacgcatta tttatctcca ttcctttcct ctaattaaaa
     9181 aggtctggcc aaatctccgc agagtttggg ccaaacctca gccactgaaa attcgtttta
     9241 attgtaaaat ttcttcgttc atccgtcgat gatcttcacg tttgcaatat ctggtgattt
     9301 cttggagcaa attcatggcc gcataccaat gaaatttttc aagcatttcg tcagtcgggc
     9361 gtatgccata actgatcagc cactgattcc agctagacag tggcacataa tttcctaaaa
     9421 tcgttgcaat atccaccgca gggtctgcaa acatgattga atcccaatcg actagatata
     9481 aataattttt acatactaac caattacgat ggtttacatc tccgtgtact actgataagt
     9541 tttccttatc tattgacggg atctgctcac gaagaaaacg atggacaatt tgcagaaatt
     9601 gatttttctg gagttcttcc ggtaattctt tttcatattt ttgcagcatc ttctccggcg
     9661 tcatgacacg accaccgata cgggtcagca tggttttaag agaatttgaa tgatgtaaat
     9721 ggtacaaaac atcaatcacg tcgtttcttt gaccaatctc attaggagtc agcaagcgac
     9781 cttccagcca ctcttgtgct gtcaaagtat cgccattccc cgttcgtttt gtccaaacaa
     9841 gttttggtgt aattccttcc cttgaaagag cagccaacat cggagtcgtg tttcgtttaa
     9901 taaagacttt gtcctcatca cgcatgccta tgtatgtttt tcctgtgtcg cctttgatgg
     9961 gacgtaaccg ccactcttgg tctaactgaa actccatcat gcattctccc gtcctcttac
    10021 tctttgtttt gcagtactta ttattctagt cgcctgaaca ctacacgtca agcacatata
    10081 cgttcttttt catttaattg ccattcaaaa aaccagcttt tctcaaaaaa aatagaacga
    10141 acagcattct tactgtcaac gctctatttt tcattctcat ttttacccta cacagctttg
    10201 ttttcagtct gacatcttct tcaaacgagt tggtacatat agtttaacaa agagcatagt
    10261 aacaatcaaa taagccgcta gcggaaccca tcgctgctgc ccatctaata cgactgcagc
    10321 aatcaaacca aaaataaccg acgcgataat taatacccat gtgagtagat actgaatcgc
    10381 ctgcttcttc tgattcttat ggattggata aagctgaacg agaaccatat agcggaactg
    10441 gttcgctaaa ggtaacagtt ggaaaccgat caaatataaa aataagaaac cgataatcag
    10501 actaaaataa aagtcgacta ctccggcaat caataaccca cctataatta ctaatcggaa
    10561 ataaagacca ctaaaatcag aacctcttgc caatcttctt gcaaacaagt aaagataagt
    10621 gttttttgat tctcttttaa tatgagtcaa tataggatcc aaatagcgtc ttcttttgat
    10681 ctttgccgta atttcaggaa catctgtgaa taaattgata aattgataga tctgatgcaa
    10741 cctatgttct tctacatgca ccattttctc ccaatccaat cctttttctt gttgcttcca
    10801 caaaaaccag taatatagac ttgcctgtac taatgcacct gctattcctg cccatattag
    10861 ccaataaaat gatagaaaca aaaagatcag agaagaaaaa aaccagccta atttccaata
    10921 acgaataact tgttgatcta gtcgaaacaa ttctgctctc ttgatttgca gatgactgaa
    10981 tttcaaactc cataatatga tcagatagac gaaaaacatc gaaaaggatt gctccgtact
    11041 aaccaccact aatggcatgg ccaatccgca tgccaatatc aaaaagatca acggaaaaat
    11101 gcaagaatag cgaaaagctc gttccaaata ggtttgcatt tctttttctt taggaagtaa
    11161 aaaaacaata tctgctgcct ctgcaaaagt agccatctta cccacgaata aacagagcat
    11221 ccaaaataca gagattatga gccctccaat tgcaaacggt ggctgcaggg ttttcaacca
    11281 attagaataa tataatccca caccgccaat caagaaaaga caaactaaaa caaaatgatc
    11341 gttcaataca taacgcatat atttcatcat tttcttttga tgtcttgcta aacgtttttg
    11401 aaaaaaacta ctcatgtgcc tgctcctttg ttaaagcaat atagatttcg tccaaagaag
    11461 aatccggcag ctgaaattct tttctcaaca tctcaagaga accgtctgct cgtatctctc
    11521 cgttatgtaa gaccacaaaa cgatcacaat atttttctgc agtagctaaa atatgcgtag
    11581 acatcaaaat accggctcct tcttttttca tttctaccat caattcaagc aatgcattga
    11641 tagctagtgg atccaagcct aaaaatggtt cgtcgatgat atataaagaa ggctcgatca
    11701 aaaaggcgca taagatcatc actttctgtt tcattccctt tgaaaaatta gctgggaacc
    11761 agtcaagacg attagataac cgaaaagtct ccaacaactt ttctgctctt ttcattgcta
    11821 tatccatagg tatatcgtag gccatcgcag tgacttcgat atgttctctc aatgtcaatt
    11881 cttcatataa agaaggagtc tctggaatat acccaatttg tttgcgatat tcttccgtat
    11941 ttttctttaa ggatagatga ttgatttcta tcgttccttt tgaagcttcc agcagaccga
    12001 taatgtgctt gatggtcgta cttttccctg ctccgttcag tccaatcaac cctactatct
    12061 ctccaggctg tacatcgaaa ctgatatctt ttaacacagg gagatgtccg tacccccctg
    12121 tgacatgatc gataattaaa ctcattcttt ttcctccgcc atttattttt atttattata
    12181 ccacgaagaa aatgacggta ttttgttttt ttccttacaa tagctttaac ttatggtaaa
    12241 gttaagctga agtatttatt ctttatagaa aggatgtttt atatggaaaa ctgtattttt
    12301 tgcaaaatca tcaatcaaga aatcccaagc tataaaatat atgaagacga caaggtctat
    12361 gcttttttag acatctctca agcaacgaaa ggacacacat tagttgtgcc taaacgtcat
    12421 gtagcagata tttttgaata tgatcccgaa cttgcaggag aggttttttc gcgtgtgcct
    12481 aagattgctc aagcattgga aaaagctttt ccagaaatga aaggtcttaa tatcttgaat
    12541 aacaaccgtg aactagccta tcaatctgtt tttcattcac atatccattt agtccctcgc
    12601 tacagcaaag aggatgattt ttctattcac tttgtcaatc atcaagatag ttatggatcg
    12661 gaagaactaa aagctattca agaaacaatc gtaaagcagg tgtcctgcga tgattaaaaa
    12721 ctttttcaaa ggactgttat ttggagctgc tgctggcact gtcggaggtc tgttattggc
    12781 gcccaggagc gggaatgaaa caagaaagaa actgatcgaa gaattggaag aagcaacaac
    12841 tctgacaaat gatttaaata acagcctgaa tcattttaaa agcgcattga tcgaaacaaa
    12901 agaaacagct gaatctgtga ttcctgcttt ccaagaagca atcaaaaaag atatcgaaga
    12961 ttttaaattc caagccgaac cacgtatcac acaaatcaat gaacaagtag aaaaactaaa
    13021 agctgatctg ccagagtatc ctgaacaaat ataataatga ttattttctc aacagattta
    13081 actatataag tgagaaaagt ttgcgtattt cctaggaatg tgatgaaatt ttgaattttg
    13141 tttgaaacta ccacgacaac gtttttaaag gaaatgggat atggtatagt taatatataa
    13201 aaataataac taattacagg agtgcaaatt ttaatgaaaa aaaaatcaat tattttggca
    13261 gctactagtg cgttggctgt tttaactcta gcagcttgtt caggcgatac taataaagat
    13321 atcgcaacga tgaaaggcgg aacgatcact gtatctgatt tctatgatga agcaaaactt
    13381 gaatcatcga accaatcctt agtacaacgg atgatcattt ataaagtatt caataacaaa
    13441 tatggcgata aagtaaccga taagcaagtg gatgcagaat acgacaaaca agcgaaatca
    13501 ttaggtgata cttttgaatc acaattagaa gctgctggat atacgaaaga tacttataaa
    13561 gaatatattc gtaacaattt agcttttgaa gctggcttga aagctcacgt agatattaca
    13621 gatgacgatt tgaaaactgc ttggaaatct ttccatccag aagtagaagc acaaatcatc
    13681 aaattatcaa gtgaagatga agcaaaagat gtgaaaaaat cagcggatga tggagatgat
    13741 ttctctaaat tagccaaaga caaatcaact gatacagaga caaaagaaga tggcggtaaa
    13801 gttaaattcg actcaacgac aacaactatt cctgcagaag taaaagaagc agctttcaaa
    13861 ttgaaagacg gtgagatctc cgatgttatt actacaacaa atcctacttc ttatgcgaca
    13921 gaatactacg ttgtcaaaat ggtgaaaaac caaaataaag gcaatgacat ggataaatac
    13981 aaagatcaac taaaagatat cgctactgaa actaaactaa gtgacaatgc attcacaact
    14041 aaagtaatcg gtgaagaatt aaaagatgca aatgtaaaaa tcaaagatga tgcctttgaa
    14101 aatgtgttga gtgcattcac aacaacttca tcaagtacga aagactcaag tgaaactaca
    14161 gcttcaacaa agagttctga cacaaaatca actgattcaa caaaagaatc ttcaactgaa
    14221 gaaacaactg attcatcaaa ataaaagaaa acaaa
//
LOCUS       DAHUVV010000068        13860 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.069, whole genome shotgun sequence.
ACCESSION   DAHUVV010000068 DAHUVV010000000
VERSION     DAHUVV010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.069"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            28..2193
                     /gene="acm"
                     /locus_tag="NK767_001996"
     CDS             28..2193
                     /gene="acm"
                     /locus_tag="NK767_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HCH6694985.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2465..2950)
                     /locus_tag="NK767_001997"
     CDS             complement(2465..2950)
                     /locus_tag="NK767_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HCH6694986.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3040..3385
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3430..4071
                     /locus_tag="NK767_001998"
     CDS             3430..4071
                     /locus_tag="NK767_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCH6694987.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4090..4725
                     /locus_tag="NK767_001999"
     CDS             4090..4725
                     /locus_tag="NK767_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCH6694988.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4743..5558
                     /locus_tag="NK767_002000"
     CDS             4743..5558
                     /locus_tag="NK767_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCH6694989.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5877..7313
                     /locus_tag="NK767_002001"
     CDS             5877..7313
                     /locus_tag="NK767_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCH6694990.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7502..9949
                     /locus_tag="NK767_002002"
     CDS             7502..9949
                     /locus_tag="NK767_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCH6694991.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10179..11246
                     /gene="rlmN"
                     /locus_tag="NK767_002003"
     CDS             10179..11246
                     /gene="rlmN"
                     /locus_tag="NK767_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCH6694992.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11439..12542)
                     /locus_tag="NK767_002004"
     CDS             complement(11439..12542)
                     /locus_tag="NK767_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCH6694993.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13095..13643
                     /gene="aac(6')"
                     /locus_tag="NK767_002005"
     CDS             13095..13643
                     /gene="aac(6')"
                     /locus_tag="NK767_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCH6694994.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4746 a         2307 c         2933 g         3874 t
ORIGIN      
        1 ttagttaaaa taggggagat agaaaaaatg aaaaagtgta gaaatattat tttatcaatg
       61 ttatttatta ttactaatat aacatcactg attcctgttc atgtctatgc ggatgcaggc
      121 agagatatca gcagtaatgt cacttcgttg accgtggacc caacgaatat tacagatgga
      181 ggaaacataa aagtaaaatt ctcgtttgac gaaaaaaaac aaaatatcca accaggtgat
      241 tatctttgga tcaattggcc aagtgaagga aatattcgcg gtgaaggatt ccaaaaagaa
      301 atcccattaa tgattgaaaa taaaaatgtt ggaactttaa cggtcagaaa agattctgcg
      361 caggttgtgt ttaatgaaaa tattaaaaac ctagactcag tagaaggttg ggggcaattt
      421 gagatacagg ccagaaacgt aaccgatacc ggtgaagaaa atacgggacc attcaccgtg
      481 acgagcggtg ataaaacagc tactgtaaat gtgacaaagc cagcttctgg ttcttcttct
      541 agcgtttttt attataaaac aggagacatg ctacctgaag atactaagca cattcgatgg
      601 tttttgaata ttaacaataa cggcacttat gttgaacaac cagttaaaat cagtgatgaa
      661 atccaaagtg gacaaagatt agatccaagt acgtttgaaa ttaatcaaat acacttaggt
      721 gaacagaagg tttatagagg agaagaggga atccaacaat ttttacaaga tttccccagc
      781 gctacattta atttttcagt aactgataat tacattgaaa taactatacc aaaaaacttt
      841 gtcaatctta ggaaaataat ggtgagttat aagacgatca tagaaaaccc agaacaaata
      901 aattttgaga atcattcgga agcttggttt aaagaattta ataaacctgc tgttgatgga
      961 gaaagtttca atcatacagt taaaaatatt tcagcttctg gtggagtcaa cggtacagtt
     1021 agaggcgaat tgaagatttt caaatatatc aatgacacag agattggtat cccaaatgtg
     1081 actttcgaat taagacgagc agatgaacag cctattcaag gacagtctag tatcctgttg
     1141 acttcgaatg aacagggaga agctagtatc aaaggcctgc aagtaggaga ctatgttgta
     1201 aaagaaaaag aagcacccaa ttggattgat tttgatccac taagttcaaa tgaactcaaa
     1261 ttctccataa acgaaaatga tacagaggga gtcagtttac ctatttataa caagaaaaag
     1321 gttacgaaca tcacagcaac taaaatttgg aacggtggaa ccactccaag accgagtatt
     1381 tattttaaat tattcagagc aagtacaaac aattgggaac cagttcctga tgcagaaaca
     1441 aaacgattag acaatggaat aacttcagtg acttgggaag atatccaaca gtatgatgat
     1501 agtggaaacg aatatacgtt taaagtgcaa gaagtagatc aaaacggaaa tgattatgtc
     1561 ccttcaggtt atcaaaaaat tgaaaacggg ctagtcgtta caaatgagaa taaagaagtc
     1621 atttcagtct caggacaaaa aacatgggaa gacaatgaga accaagacgg caaacgaccg
     1681 acgacgatca cagtaaatct attagccgac ggtcaaccga tccaacacaa agaagtaagt
     1741 gaaaaagatg attggcgcta tcgtttcaca aatctgccaa aatacagaga cgggcaagaa
     1801 atcatctata ctgtaacgga agacaacgtg cccgaataca gtacgacgat tgacgggtat
     1861 aacatcaaaa acagttatac accggaaaaa acaaatatat ctataccagg tagccatata
     1921 acaccgtgga aaaattttcc agagaagaca gagagagaga aaattagaaa tctattctct
     1981 caaaaattgc aaatatttgc tcatacagga gaatccagcg aacaaagaag caattatatt
     2041 agtaagagag ctgtacctaa acagataaca aataaatcaa aaagcattct tcctaaaaca
     2101 agtagtaaaa aatctagttt tgctgtaatc attggtttat tgatagtaac tatctgtgct
     2161 gggatattct tacaaaaata taaaaaagtt tagttagaat ataatatttt ttgttaaata
     2221 tgactgaaat caaaagaata aaattccgat ttataatcga aaaaagaagc atttttatat
     2281 atgcttcttt tttcgtagaa tcaataaata tggatttggc taactatgga agaatactcg
     2341 tattagtttt cagcgttcat actagttgtt acattgtttg attggccaaa agaatttctg
     2401 atatattttg tgctaaataa agaaaaagac gcttgaattg ggaaataaga aaactactct
     2461 atcattacaa attttcataa actggcagat gttctaagta aacaagtatc ttttcaaagt
     2521 cggaaaaatc cataatcaac gtagtggtat tgatatttgg atgaaagcct acggtatctt
     2581 gacgatcaat agcagcatcg atcacgactt ggattttttt ctctttgtcg tgcatcaatg
     2641 ctaatggagt cactgtacct gctggaacgc ctaggaggtc aggaagctgt tcttcagaaa
     2701 caaaagataa gcgcttttct tttaattgtt cagctagttg ttttaaatcg gcgtgctttt
     2761 catcaggcaa gatgacaaga tagatttgtt ttccttttct tgatttcaat aatagatttt
     2821 ttacttgcgg accaggtagg gaaaaaggga catttttgac tgacgtaatc gcaggatgat
     2881 cgactttttg aaaagagata cccagttttt ttagtagttc ataagcttct tgttcggttg
     2941 cacgactcat cgcgcatcac cctccttttt ttattagtgt atcataaaaa agagttgaca
     3001 aatgaaaaat aatcatgtaa attacacgta aagttaaaac aaatgacatc gaaaagaaga
     3061 gtagccgtta ggtgttttta gagagtctct ggttggtgga aagaggcagc agttaaccgt
     3121 gaaacacatc ttggagtcga ttttctgaag cgtatagtag gaaaatccgg ggagcccgtt
     3181 acagctgcgg tcgttgctag atgaccagat aaggctggct tttccgtgag gagtcggtaa
     3241 aaaaaggtgg aaccacgtga ttaacgtcct ttgatcttcg gatcaaagga ctttttgttt
     3301 tcatctgaac ggcaacgact gtgtgaggtt ttttctgcaa ggaaaaaata aacagaggtg
     3361 gaaccacgta taatacgtcc tcttggcatt tgccaagagg atttttttat tttaaaggag
     3421 gaaaagaaga tggagtattt gattgaagtt atgccgcaat tattagtagg agcactcaca
     3481 acattgaaat tatttttatt taccttgatt ggctcattac cattagggat tgttttagca
     3541 ttaggattgt ctttgcattt taagccgcta cgctacttgc ttcaaacata tgtatgggtt
     3601 atgcggggaa caccgctgtt gttgcaactg atttttattt tttatgggtt gccgacgatt
     3661 gggatcgttt ttgatcgttt cgaagcagca ctcattgcct ttatcttaaa ctatgcagct
     3721 tattttgcag agatctttcg tggagggata caatcgattc ctcaaggtca atatgaagca
     3781 gcgcaagttt tgcaattaag tccctttcaa acggtgacga aaatcgtcat cccacaagtg
     3841 acaaagattg ttttaccttc tgtagggaat gaagttatca acttggtcaa agattcttcc
     3901 ttgatctata tattaggttt aggtgacgta atgcgtgctg ggaaaatcgc gatggaacgc
     3961 gacgcgactt tactgccttt gctaggcgtg gcaatcatct atcttacatt gactggtatt
     4021 ctaacaatca taatgaaaca agttgaagga aaattaaatt attaccgcta actgatagga
     4081 ggaagaaata tgattgaatt gaagaaagtg aacaaagcat ttggtgaaaa aaaaataatc
     4141 aaagatttga atctaatcat tccagatggg aaaacattag ctatcgtagg tccttccggt
     4201 ggcggtaaaa ccacgctttt acgtatcttg gctggcttag aaagttctga cagcggagaa
     4261 ttttatttag acggtgtacc gttcaaccca ttcgagctta aaatgaaaga acaagttgta
     4321 ggagtcgtat tccaagactt ccagctattt ccacatctga atatttttga aaacattacc
     4381 ttagcaccaa aaatggtatt gaaacagaca aaagaagaat acaccaaaaa agcagaagaa
     4441 ttagcggctt cattagggat tagtgattta ttgaatatct atccttatca gctttctggc
     4501 ggacaaaaac agcgggtagc gattgccaga gcattggcta tgaatccacg ggtattggct
     4561 tatgatgaac caacaagtgc tttagatcct gaattacgcc agcaagtcga gcgtttgatc
     4621 ctatcattga aagaagatgg ggtaacccaa attattgtca cccatgattt agtatttgca
     4681 gaaaatgtcg gtgatcaatt actaaaagtt tccccaattc attaagaaag gagaagatag
     4741 agatgaaaaa aaccagtttt ctattcgctt tgatcgcagg tcttcttctt tttgcaggct
     4801 gttcgaacaa aaaaacttca gcagataatt gggcgactat caacgaagag aaaaaaatcg
     4861 ttataggttt agacgataca tttgttccta tgggatttag agataaagac gggaagctca
     4921 caggatttga tattgattta gccaaagcag tctttgaaca atatgggatt acggtagact
     4981 ttcagcccat tgactggtca atgaaggaat ttgaactgaa caatggaacg atcgatttga
     5041 tatggaacgg ctattccaaa acgccggcaa gagaaaagaa agtgcaattt acaaaacctt
     5101 atatggaaaa cgatcaagtg ctgatcacac ctaaagaatc aggaatcaca aactttggac
     5161 aaatgaataa taagagatta ggtgcgcaaa atgggtcatc tggttatgat gtatttacga
     5221 aacaaccaga agtattaaaa gagatagtca aaaaccaaga tgctgttttg tatgatagtt
     5281 ttaatgaagc attgatcgat ctgaaaagca gacgggtcga tggtctgctg atggataaag
     5341 tatacgcaga ctattatttg aaacaaagaa acgaactaga agacttcaac ataacaaaag
     5401 gttcttatga gagtgaggat tttgcagttg gtgcacgcaa gtcagatagt acattagtga
     5461 aaaaaatcaa tgaagcccta gatcaactag agaaaacagg tgaattccaa aaaatatccg
     5521 ataaatggtt tggcgaagat gttacgccga gaaattaaca aataggggac tgtgacaaaa
     5581 gacgtgtcac agtccctttt tccgaataaa cagtgttctc ctgccaactc cttgtgactc
     5641 ggagctgaat ggcaggctca caacctcttg gtaaacggtg ttcaaaagcc agtttttcgg
     5701 tattaagcca aatttatgca aaaatgtaga aagccatttt cacaaaattt ttcttactac
     5761 tcgaaactaa gcgacttttt cacaacctcc tttacataat tttattttgt tgtggaaggg
     5821 gtttcgtgtt attctaaaag aggaacgatt gaaggttata ggaaaggaag agatgaatgg
     5881 aacacaaaga acttacctca tttccaaaag attttctgtg gggctctgct tctgcagcgt
     5941 atcaagtaga aggagcttgg caagaagatg ggaaaggaga atcagtctgg gatcgatttg
     6001 tacggatacc tggaaagaca tttaaaggaa cgaacggtga tttggcagta gaccactatc
     6061 atcgttataa agaagatatt gctttaatga aagaacaagg tctgaaagct taccgcttct
     6121 ctgtagcttg gacacggatt tttcctaatg gacgaggaga agtcaatcaa gctgggctcg
     6181 ctttttatga gagattgatc gacgaattga ttgaaaacga tattgaacct atgttgactt
     6241 tatatcattg ggatcttcca caagcattgc aagatgaata taatggttgg gaatcacgtc
     6301 agatcattga agatttcact cattatgcag aaacattatt tgaggcattc cgcgggaaag
     6361 tccattactg gattagtttg aatgaacaaa atatcttcac ttcattaggt tatttgttag
     6421 cagcacatcc gccaggtgtg acagacccga aacgaatgta tgaagttaac catattgcta
     6481 atttagcaaa tgcatctgtc atcaataaat ttcacgagtt agaaatgcct ggaaaaatcg
     6541 gaccaagctt tgcgtatacg ccaaattatc caattgacag taatcctgaa aatgttctgg
     6601 ctgctgaaaa tgcagaagac ctgatggcca attattggat ggatgtctat atgtggggaa
     6661 aatatccgat tgctgccatg aggttcttag aagaaaaagg atgggcacca acgattgaag
     6721 ctggcgatgc agaattgcta gaatctgcta aaccagattt tcttgggatc aattactatc
     6781 aaacggcgac gaatgcattc aatccattaa acggagttgg tgctggaaaa atgaatacga
     6841 caggaaaaaa aggttcatca gaagaaactg gagttcctgg aatgtataaa aaagttgaga
     6901 atccatttgt cgaacgaaca aattgggact gggagattga cccagaagga ttaaggatcg
     6961 gactgcgtcg tatcacaagc cgttaccgca tgccggtact gattacggaa aatggcttag
     7021 gagagtatga caagttgaca gaagataaac aaatccatga cgactatcga atcaattact
     7081 tacaaagcca cgtcaaagcc atcaaagaag caatttcgga tggagcagaa gtcctaggct
     7141 attgcacctg gtcttataca gatttgctta gctggctgaa cggttatcaa aagcgatatg
     7201 gatttgtata tgttgatcaa gacgaaacac aagaaggatc tttggaacga tataagaaag
     7261 acagttatta ctggtaccaa aaactgattg aagaaaatgg agagaacctt tagttcacat
     7321 acttaaaaca agaaggtacg gctggatatg gccgtgcctt ttcttgataa aatcatttag
     7381 aaattaagaa aaactgtaaa ataagctgaa aaatgaaaga aagcgtcagt tctttcaaac
     7441 aaaactttag ttcccttact tttttttgta taatgaaact tgatgaaatg agggataata
     7501 aatggcacaa aagaaacggc ctactaaaaa aaggaaaaag acgaaaaaaa cgaaaaagca
     7561 aaaacagcag caggaacaaa tgctgacgat tggtgttggg tttgccttga ttttatttgc
     7621 tgtcttcggc tttttaaaat taggtttttt aggaatattg attgcaaacg gctttcggat
     7681 cattgcagga aatacttacc aaatcctctg tcttttatta gccgtattag gattttggat
     7741 tgtcattaag aatacagagt tttcaattgg taaaaaccga cgctggtttg gcggcatact
     7801 cttttatttc ggtatcttat tattgttaca tgcccattta tttggcaaac tccatactgg
     7861 ggaaccaaat atcatgggga cgacatggga tttgttagcc agtgatataa aacaaagtca
     7921 agtagacaat aacgttggtg gtgggatgat tggagcaatc ctttatcatt ttacttactt
     7981 cttgattgca cagcctggaa gttatttagc agcagtgcta ttactggcag gaggagcatt
     8041 cctgttcagt aatctggaag gctatcagct gttaaatggt atccaatcaa ttggagaacg
     8101 agtacaggaa ttattggaag gtgatcctgc caagcaagct cgaaagcaag cggcaaagga
     8161 agagagaatg aaacagcgcg cagaagcgaa agaagcaagg cgcttagctg caaaagaagc
     8221 tgcagaaaaa gaagctgcag aatatgagaa gaaaaaagcg gtgagccagc aaagggaaaa
     8281 tactgctgat gaatggcagg aaccagaaaa tacagagccg gagcagctaa gttttgttcc
     8341 aatcgacagt tttcaagaaa atatccatcc tgcgaatcta gagaaaccag taccagatac
     8401 accaaaacag accaatacag cagaaggttt ttcagatgag ttgccagagg atgacggtac
     8461 ctcattagaa tttgagattg aagcagaaca ggaaaatcaa gactatgaat taccgacagt
     8521 tgatttgctt gattctatac caactgtgga tcaaagtgat gagtataaaa aaattgaaaa
     8581 aaatatcggc gtattagaac aaactttcca aagtttcggt gtcgatgcca aggtcgtcaa
     8641 agccagctta ggaccatccg tcacaaaatt cgaagtccag ccggctgtag gggtaaaagt
     8701 aagtaaaatt gtgaacttaa cagatgatat cgcgcttgct ttagcggcga aagatgtccg
     8761 gatggaagca ccgattccag gtaaatcact gattggtatt gaagtaccaa atggaaagat
     8821 cagtatggtt tcctttagag aaatcatcga agcacaacca aaccacccag ataaattact
     8881 agaagtacct ttaggacgtg atgtttctgg acgggtacaa acggctgatc tttcgaagat
     8941 gcctcactta ctcgtggctg gttcgactgg tagtgggaag tcagtagcaa ttaatgggat
     9001 tatcacaagt atattaatgc gtgctaagcc ccatgaagta aaactgatga tgatcgatcc
     9061 gaaaatggtt gaattgaata tgtacaacgg catacctcat ctgttgacgc cggtcgtgac
     9121 gaaccctaga aaagcagcgc aagccttaca aaaagtagta caagaaatgg aagaacgata
     9181 tgaaaaattc gcggcaaccg gagtaagaaa tatttctggt tacaatgaat ttgttcaaca
     9241 gaaaaacttg gaaaacggaa cgaaacatcc aaccttacca ttcatcgtag tgatcgtaga
     9301 tgaattagct gatttgatga tggtagccag caatgaagtt gaagatgcga ttattcgttt
     9361 agcgcaaatg gctcgggcag cggggattca tatgatccta gctactcaac gtccaagtgt
     9421 ggatgttatc acaggaatca tcaaagcaaa tgtaccttcc agaatggcat ttgcggtatc
     9481 aagcggtaca gattctcgga cgatcattga ttctaatggg gcagaaaagc tcttagggcg
     9541 aggagatatg ctattcttgc caatgggtga gaataaaccg atccgtgtgc aaggagcatt
     9601 catttctgac cacgaagtag aacgggtcgt tcaatttgtt acagatcagc aagaagctca
     9661 ttatgaagag aaaatgatgc cgacagatga agttgaaacg acaggtgctc cagaacagcc
     9721 acaagatgag ctgtttgaag aagcaaaagc acttgtcgtt gaaatgcaaa cggcaagtat
     9781 ttcccttctc caaagaagat ttcggatcgg ttacaaccga gcagcacgtt tagtagatga
     9841 actggaagct catggagttg ttggcccgtc agaaggaagc aagcctcgga aagtattcat
     9901 tgagcaggaa gaaacatcag aaacgttgcc ggacgataat gacatgtaaa acttgtgtga
     9961 tgaagggttg aaaaacgaat aaccaataaa aagacaaggc atgatgatac caaaagaaaa
    10021 aatggttttt ttccatcttt tctaatacgg tatgatgtct tgtcttttta gtttttgtga
    10081 gattaaagtt ctattcgatt tatttgtgaa gtattgcatt aaggaaatga atcatgtata
    10141 gtatgtagcg aaactttgag aaaaagaggg aaaatacttt ggaaaaacag tccatttatg
    10201 gcttgacgaa tgaagaatta atcaattggt ttatagaaaa cggcgagaaa aaattccgag
    10261 ctgcccaagt ttgggaatgg ttgtatcaaa aacgtgtaag taatttcaca gaaatgacga
    10321 atctatcgaa acaattgatc gaaaaattat cggagcattt tatcatcaat cctctaaaac
    10381 agatggtcgt ccaagaagct tcggatggta cagtaaaata tttgtttgaa ctccctgata
    10441 aaaacatgat tgagactgta ctgatgagac aagaatatgg cttgtcagtt tgtgtgacga
    10501 cacaagtagg atgtaatatc ggctgtacgt tttgtgcaag cgggctgctc aaaaagaacc
    10561 gtgatctgac tgctggagaa attgttgcac agatcatgat ggtccaacat tattttgatg
    10621 agcgcaatct tggcgaacgc gtttcacatg tcgttgtaat ggggatcggt gagccatttg
    10681 ataattacga caatgtaatg gactttttgc atatcatcaa tgatgccaaa ggattggcaa
    10741 ttggtgctcg gcacattaca gtttccacta gtggattagc acacaaaatc aaggaattcg
    10801 ctaataacgg cttgcaagtc aatctggcta tttccttgca cgcgccgaat aatgaggtgc
    10861 gaacatcgat catgcggatc aaccgaagtt tcccaattga gaaattgatg gaagctgtgg
    10921 atgagtattt agagaaaaca aaccgtcgaa tcacgtttga atatatcatg ttgaatcaag
    10981 taaatgatag accagagcat gcgcaacaat tagcagatct attaaaagat aagaaaaaat
    11041 tagcatatgt caacttgatt ccatataatc cagtcagtga acatgatcaa tattctcgca
    11101 gtccaaaagc tgatgtattg agattttatg atgtattgaa gaaaaacggc gtaaactgtg
    11161 tgatacgtaa agaacatggt acagatattg atgcagcttg cggacaattg cgaagcaagc
    11221 aaatgaaaaa aacagttgcc aaataatcgt atcctactcg ttttttcaat taccataaaa
    11281 ggagcagttt tcttaattaa ggaactgttc aacttaaata agagaaaaga ttctatagcg
    11341 aaagctataa agcttttttt gacaagaaaa accctcatca agcaaagaaa tgtcctcaat
    11401 ttgatgacag cctgcttgat gggggtttta gcaattattt aactgatttt cttatatcct
    11461 tgctggcttt cttgtgtagc atgataagtt tctacgtcaa gaatggcctg tttaaatttt
    11521 tctcgggtga tttcataagg cacatgttta atatcatttg ttgccatggc tttatctaag
    11581 attgcttcta aatcggtcgt gacattgaga ttcatgtcgg ttaacgatac tggaagttgg
    11641 ttctttttca taaacacata gattttatca aacaaatcga actgctgatc catcatcaat
    11701 aaggtcagta caccataact gacagatgct ccgtgaaggt atccctccat tttttctaga
    11761 acagtcgtac cgtaatagaa tgagtgtgcc atagcagaat tatagttatt gttaactaaa
    11821 acagaaacca atccggtagt aacaatgata tccgaaacga cttgttcaat tgcatatgag
    11881 ggacgatcgt cggcactatc tttcaatgct tgcgtaccat aagtcaagag acgatcaatg
    11941 cacatttttc cgatttggat tcctaatcta tccgtgtagc ctagatcatc gccacgagca
    12001 gaaaaagtag attcgacttc tttggataaa gcatcgccaa tacctgccca taagtattta
    12061 tcaggtgctt cggcaataat tgctgtatca atgaaggtgt gtacaggtgg ttttgaagga
    12121 aagaagagtc cgtccattac gccttcttcg ttatagatga cacataccgc agtgacgggc
    12181 gaacagttcg aaccaatagt gggaaaggtg aagactggtt tagctagttt ttcaccaatg
    12241 acttttacag tatcgcaagc acggccacca ccgactgcaa acaggacgtc tgctttttgg
    12301 acactttcat gctggatcaa ctgttcaata tttccataag ttgaattacc cccgtaccag
    12361 agactggcga caacctctat ttcctgtgtc aaagctactt ttattttgtt ttctgccttt
    12421 gaaagtgcag tcttccctcc aatcagcact acttttgtcc catattcttt gacgattcca
    12481 tttgcttttt catacgcatc tgttccaatg gtataatttg gtaagaaaac actttgactc
    12541 atattcattt ccttctttct tcgttgaaac gggttaaaat tttaaagagt gatcgatctg
    12601 actgcggctg tagatggagt agaggtgaac ctaagacagg ataagtgatg aataaggtgg
    12661 catgaacacc ttgaaatcat caataagtat cctaaaaacg aacaagtaca aaaacattga
    12721 aaacgacaat aatgaatttt tgttcctctt agtatttcta ccgattccct ttgctttttt
    12781 gtaattatct aaaaattcta aaaattaaga ttattatatg aaaacaggtt ttatttgtca
    12841 attcataatt ggtttttttt aaagcaaaag ataatctata tcatgatgaa caaacagagt
    12901 tttttcctcc aattgatggc tttcctgatc tttcctattt tgaataaagt gtttctgtat
    12961 aattttttta ttttataagt aagataaata atgaaagaat agagatactg attggtagct
    13021 aaagattttt gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac
    13081 aagggggaag acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc
    13141 agctttctga tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag
    13201 aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt
    13261 tagtaggatt tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat
    13321 tagttgtaga aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag
    13381 aaaaagaagt agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc
    13441 atggaacaac gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca
    13501 tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag
    13561 gtgtcttacc aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta
    13621 ttcctcgacc aaattctcaa taataaaaag aggttgtgac agaagtaatc agcttcaaga
    13681 aataagaagg aatttacgaa aattacttct tgaatttttg tgaaattcca acttatttcc
    13741 gaagaagcta cttttggaac accgtgtacc agaggttgtg aaaaagtcgc ttagtttcga
    13801 gtagtaagaa aaattttgtg aaaatggctt tctacatttt tgcataaatt tggcttaata
//
LOCUS       DAHUVV010000069        13838 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.070, whole genome shotgun sequence.
ACCESSION   DAHUVV010000069 DAHUVV010000000
VERSION     DAHUVV010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.070"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(4..534)
                     /locus_tag="NK767_002006"
     CDS             complement(4..534)
                     /locus_tag="NK767_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCH6694995.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(633..983)
                     /locus_tag="NK767_002007"
     CDS             complement(633..983)
                     /locus_tag="NK767_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HCH6694996.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1140..2669)
                     /gene="ahpF"
                     /locus_tag="NK767_002008"
     CDS             complement(1140..2669)
                     /gene="ahpF"
                     /locus_tag="NK767_002008"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCH6694997.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2679..3242)
                     /gene="ahpC"
                     /locus_tag="NK767_002009"
     CDS             complement(2679..3242)
                     /gene="ahpC"
                     /locus_tag="NK767_002009"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCH6694998.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(3386..4048)
                     /locus_tag="NK767_002010"
     CDS             complement(3386..4048)
                     /locus_tag="NK767_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCH6694999.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4232..5878
                     /locus_tag="NK767_002011"
     CDS             4232..5878
                     /locus_tag="NK767_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCH6695000.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5869..6951
                     /gene="uxuA"
                     /locus_tag="NK767_002012"
     CDS             5869..6951
                     /gene="uxuA"
                     /locus_tag="NK767_002012"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCH6695001.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(7059..8612)
                     /locus_tag="NK767_002013"
     CDS             complement(7059..8612)
                     /locus_tag="NK767_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCH6695002.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            8760..10048
                     /locus_tag="NK767_002014"
                     /pseudo
     CDS             8760..10048
                     /locus_tag="NK767_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(10174..11253)
                     /locus_tag="NK767_002015"
     CDS             complement(10174..11253)
                     /locus_tag="NK767_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCH6695003.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(11356..11796)
                     /locus_tag="NK767_002016"
     CDS             complement(11356..11796)
                     /locus_tag="NK767_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCH6695004.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(11853..12263)
                     /locus_tag="NK767_002017"
     CDS             complement(11853..12263)
                     /locus_tag="NK767_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCH6695005.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(12443..12877)
                     /locus_tag="NK767_002018"
     CDS             complement(12443..12877)
                     /locus_tag="NK767_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCH6695006.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(12977..13825)
                     /locus_tag="NK767_002019"
     CDS             complement(12977..13825)
                     /locus_tag="NK767_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCH6695007.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         4676 a         2581 c         2445 g         4136 t
ORIGIN      
        1 tgattaacgt atagtcattt ttcttattct gatgaaattg ataatctcct ggatgcccat
       61 gaaaatcaaa ataaagccga atatttggaa tagaagcatc aaacttttga atggattgaa
      121 caataagacg aatccgccaa tcaacaacag aatactacta taaacactcc aacttttagc
      181 ttttgtattc atgctgaaga acaactggaa caagctgttg atgataacag ctaatccaag
      241 aagaatcggc aggatagaaa cgattgcagg tgcaaaagca tagacgatca gagctaagat
      301 caggaaaaaa agaccactga acagaccgat actccaagaa cctgttttcc ttttaaaacg
      361 gtaagcctca aaaagattca acaacccaag caaaacaaga taggcggtga tcagataacc
      421 gataaaatga aaaacagcag aagggtttag cacaatcgca atcccagaaa taatataaac
      481 agcagctctt aataaggcgt atttttgaat attttgttta atcgtctcaa acatataaga
      541 aaacctcctt tgacttattt taacgtgaac tagtcatttt accaaattaa atgttacaaa
      601 atgagcgaag gagaagaata tctctggaaa aatcaacttc tgaggatctt ttccattttt
      661 ttgccttttg ctagctcatc gatcatttta tctaaataac gaatttcttt catcaagtta
      721 tcctcgatgt cttctactcg tacaccacaa acaacacctg taatcaagga acgtgaagga
      781 ttggggacag gggcttccgc aaaaaattgt tcgaaagtca cttgtttttg tagttgatat
      841 tctagttctg tttgggaata acccgtcagc caacaaatga tttcatctac ttcctctttt
      901 atacgatctt ttctctccgc ttttgctaca tacataggat aaacgcttgc aaaacttgtc
      961 gtaaaaatac gattttgcgc cataatcagt cacctttctt tttttcaaat tatttactac
     1021 ttatagtata taagaaaaag agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt
     1081 gacaaaattc ttgtcaccat ccttttttcc gctctctgac tttttacaat cttcatagct
     1141 caagtgcgca tcagataatc gaatgctcct aaagctgcag tagctcctga acccattgaa
     1201 acaatgattt gtttgtatgg actatctgta caatcaccag ctgcgaaaac gccggggata
     1261 ttcgttgcgc catttttctc ggtcactatt tcatttcttg gattcatttc gatcgttcca
     1321 tctagccaat ccgtattagg gagtaagccg actagaatga atactcctga aacagctagt
     1381 gtatgcatct ccatcgtacg gcgatcgata taggagagag attctacctg attcgtaccg
     1441 ctgatttctt tcgtcgctgc atgtttgata acagtgacat tcgcaagaga gactagttta
     1501 tcctgtaaga tttgatctgc ttttaattct ggtaagaatt ctagaacata tacatgtttt
     1561 gctaacccag ccaaatcgat tgctgcttca acaccagaat taccaccacc gataacagca
     1621 atctcttttc ctttaaataa agggccgtca cagtgtgggc aataagcaat tcctttattt
     1681 ttaaactctt tttcgcctgg tacattgata gaacgccagt gcgcacctgt cgcgatgatg
     1741 gcggtttttg ctttaagtga tataccgtta gctaagccaa tattaataaa atgttgctct
     1801 ttatttaagg aaacggcctg ttgattcatc ataatgtcga tagggtaact acgcacatga
     1861 ctttctactt gctgcatcaa ttttggacct tctgtgtaag aggtaccaat catattttca
     1921 atccctagtg tttcaacgac ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt
     1981 cctttacgtg ctgcatagat agctgcacta ctagctgctg gtccaccgcc aattactaat
     2041 acatcataag gttctttttc aaacaactct tcttcaatca aagggccagt gattttttca
     2101 acaatttgtt cgatcgtcat gcgtccgcta ctaaattctt ctccatttaa aaacacagtt
     2161 ggcactgcca tgattttttt atcatcgatt tctttttgga acatcccacc ttcaatcatc
     2221 gtatgagtaa tatttggatt taatacagct aatatattca atgcctgtac aacgtcgggg
     2281 caattatgac aagtcaagct aacataactg tcaaaatgca atgtttgtgt tatttttttg
     2341 attttaagac tcaaagattc actgattttc ggtgcccgtc cactaatttg taacatagcc
     2401 agtaaaaaag aggaaaattc atgaccgagg gggataccag caaaaacgat tccactttct
     2461 ttgccaactg gattgataga aaaactaggt gtgcgcatcg aatctctttt ttcccaagag
     2521 attttaggag acatttctgc tatttccttc gtaaattcag ctagtttttg actattttca
     2581 ctattatcca aactaacaga aaaaacaata ggagattcaa tcagtgctaa atattgttcg
     2641 agattttgtt tagtttcttt gtctaacata tccaccacct aaatctttcc aacaagatca
     2701 aaacttggtt tcaatgtttc gccactttct ttccattttg ccggacaaac ttcacctggg
     2761 tgggtacgta catattgagc agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg
     2821 attccatctg cattgatttc catagcttga acgattccgt ctggatcaat aataaatgtt
     2881 cctcgctgag ctaatccttg ctcttcatcc aaaacatcaa aaatacgaga aatctgatga
     2941 gacggatcac cgatcatcac gtattcgatt ttaccgatag catctgatgt atcatgccat
     3001 gctttgtgag taaagtgggt atcggtagag caagaaaaga cttcaacacc taaagatttc
     3061 aatgtactgt attgttcttg taagtcttct aattcagtag gacaaacaaa agtaaaatca
     3121 gctggataga agcaaagaat actccagttc cccataagat ctttttcact gacttctaaa
     3181 aattctcctt gatgataagc atgtgcatta aattcttcga tttttttccc aatcaatgac
     3241 atagtaaaac ctccaataat aattatttat ttataattat tgtaatttag ttggagttgc
     3301 aaagtcaatg gatattttgt tatttaataa agattactca aaaacgaagg ttaattataa
     3361 aaatagaaga ggtcgcaaga aaatgctact gctggaaata ctcgggataa cgttgagtaa
     3421 cagtttcctg ttctttcagc atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt
     3481 ctggtttttt atcaactaaa gccttgagta aatcataatg ttgttccatg atcgtttccc
     3541 atggaagccc ctgaatggtc actcgtaacc agcggaaacg ttctaaatgg gtattatggt
     3601 catctaacca gttccaaact tcttttcttc ctgcaatttc gaaacaagct tcatgaaata
     3661 aattatctgc acggaaaaaa gcgcgttcgt catttatttt gactgctttt tcttgttctt
     3721 ctaaaatcgt ttctagcact attcgtgttt gtgtgtccaa tttggaacaa cattcaatca
     3781 tgatttgctc ttccaactgt tctcggacaa agcgagcatt ttctgcagaa acaagattaa
     3841 ttttggaaac atacgtacca ctttgaggta tcgtatacac gagttcttgc tgacgtagtt
     3901 gaatcaatgc ttcgcgaatg ggagtccggc cgatagcaag atgttcctcc agtgcctttt
     3961 cagatatttt ccttccaggt tctaagtcag catagataat ttgtttacga atcgcttgat
     4021 aagcttgtga ttgcaagctg cttttcatct ttattcctcc aaaatcattt ctatgaacgt
     4081 agtataacag aagaggtctg aaaaacaatc tggtatgtca cattaactag taaatttgta
     4141 taaatagtaa aattttggtt gacatagcga tttttattgt ataaaattca cttgaataca
     4201 aaaactaata tgttagttta ggaggggaaa aatgattgat ttaaacaact ggaggaactt
     4261 tatccctgaa ttatctcaaa aagaagtaga aatacctcat tttgaccgtg catcattaag
     4321 agaaaatgga caaaaaaatc ctgtctggat ccatttcggt ggcggaaatc tttatcgagg
     4381 atttcatgca gaaatcgctc aacgtttagc ggatctaggc gaattgtcca ctggtgtcat
     4441 tgtttgtgaa acattcgatg aagaaataat tgaaagggct taccaagatt ttgatgattt
     4501 gattcttgaa gtggtcatgc caagcagtgg agagttcgtc aaacgtatat tagctgcgac
     4561 agctgacagt ctgtattgtc atccagattg ttctgaacac tttgataaga tgattcgaca
     4621 ctttgaaaat cctagtttac aattagttac ttttgctatt actgaaaaag gttatcgatt
     4681 gaaagataca aataataaat atataggacc gattgcagtc gatataaaaa atggtccaaa
     4741 gaagccatca catacaatga gtattcttgc tgcgctactt tataaacgtt ttttatctgg
     4801 agcttggccg attgcacttg tctctacaga taatttttca caaaacggta gtcaattacg
     4861 tacttctttg ctaacgatcg caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt
     4921 tatccaatac ttatcgaatg aagaaattgt tgcgtttcca tggacgatga ttgatcgtat
     4981 caccccgaat ccttccttgt cagttagcaa aatgttggca aaagagggct tttcaaatat
     5041 gtcgattttc caaaccgcaa aaggaaataa tattgctcca tttgtcaata cagaggacat
     5101 ccattattta atcatggaag atcattttcc caatggacgt ccgaaattcg aaaaagctgg
     5161 ggtattcttg actgatcgtg caacggtaga taaagcagat acgatgaagg tgacgacttg
     5221 ccttaatcct ttgcatacgt ctttagctat tatgggttgt ttactggggt ataggagcat
     5281 agctgaggag atgaaagatc cagatttatc gctgctagtt tctgaaatcg ggtacaatga
     5341 aggcctccca gttgtggagt ctccaggtat cattgatcca aagatctttc tggaagaagt
     5401 aattactgtg cgcttttcta atccaaatat tcctgatatg cctcagcgaa tcgccacgga
     5461 tacctctcaa aaggtggcga ttcgttatgg tgagacgatt aaaaaatata tggaaataga
     5521 agttaaagac ccgcagacac ttgtattcat accattgact ctcgctgctt ggcttcgata
     5581 ccttattggc attgatgatc aaggaaaacc attcatcccg agtccagacc cactattgac
     5641 agactttcag cataagcttt ccgatattca actgggtacg caagatacat cacatattca
     5701 tgagaaaatg gctcctattt tgtcaaataa agaaattttt ggtgtagatc tttaccaagc
     5761 agagcttggc agaaaaatag aagaaatgtt catcaagatg gtagcaggac cgaatgccgt
     5821 acgaaaaaca ttacaggatt acttacaaga acatggagga aatttatcat ggaaatgact
     5881 tttagatggt acggatcaca ggaagaaaag attaccttag atgaaatcag acagattcct
     5941 gcggtcaaag ggattgtagg cacattgatg gatataccag taggagaggt atggcccagg
     6001 gagaggatca aagaactaaa agaagaaatt gagtctcgtg ggttaacact aaaagtaatt
     6061 gaaagcgtca atgtgcatga tgccattaaa attggattac cttcccgtga gcagtatatc
     6121 gaaaactata agcagacaat ccgaaacttg gcagaatatg gaatagaagt catctgctat
     6181 aattttatgc ctgtatttga ctgggtgaaa tctgaccttg actaccgctt agaagacggc
     6241 tcatccacgt tagcttttat atctgatgat attccagatg atccaagaga aatcgtcgcg
     6301 cgcatcgaac attccagcaa tgaatttgaa ttgccgggat gggaacctga gcgcttagct
     6361 catatcaaat ccttgcttga agcctatgca tcagtagacg aagaaaaact tcgagaaaat
     6421 tttgcctatt ttctccaatc aatcatccca acatgtgaag aggtaggggt caaaatggct
     6481 gtacatccag atgatccgcc atactcgatt tttggattac ctcgaatcgt aaagaatcga
     6541 gaagatttag attggttgtg ccgcatagta gacagccctt ataacggaat cactctttgt
     6601 acagggagta ttgcagaaga tccagaaaat gatgtatatg ggattttagc agaattttgt
     6661 caaagagatc gaatcccatt tgcacatgtt cgaaatatta aatttttagc taatcgagat
     6721 ttctatgagg caccgcataa atccgaatac ggttcattag acatgtaccg catactaaaa
     6781 acaatgtatg acaacggatt taacgaatac atccgtcctg atcatggaag aatgatctgg
     6841 aatgagacag gaagaccagg atacggctta tatgatcgag cattaggtgc tgtttatcta
     6901 aatggattat gggaagcaat cgaaaaagga aacaaaagta aagataatta gtgaaaaaaa
     6961 ctgtaatttt attatgtaaa taagaaggat gttcaaattc ttgatacata aatccggagg
     7021 ttgttaataa gcattcggat ttatgtatct tatttggtct aggaagtaac ttgattttct
     7081 tttttcgatt gatccatgag tgcttcttct tttaattcaa agatatattc tgagatcctc
     7141 ataatcactt ttttccagtc ttctggtgtg atgattcctt ttaaaatata gatttttttg
     7201 atatgagaag gcaattgttt caaagggcag tcgctgataa cgatgttgca gttctccata
     7261 aaatcatttt gtattttgat cgattcattt gaaaaaattt gttctatctt aacgatcaga
     7321 tctttactga tataaaaatc acgcgtatag ttcacaccaa tcacaattcg cggctgtttt
     7381 ttgtagcgat catacaaatg tactaggtct tcaaccatcc attgcacatt ttccttttct
     7441 aaaataactg gtaacaagtg ttgattttgt tctctgaaat ccatataaaa ttgttcaatg
     7501 gcggtttgga cttctttcaa cacgctatgt tcttgataaa gcatgaaagt aggaaggggc
     7561 gtatttggtt gattgaagtt aagaaatcca ttataaagtt tatttcgtgc gaatgccaat
     7621 gagtaaagaa tcttttcttt ttcaggtata aacaaatcaa atacagtaca aaaggactcc
     7681 accatttctc ttgaataaag aacatgagga acgtttgctt tttttaaaag tttgtattgc
     7741 tccaagcttg tttcttccga ttcgattttc gtgatagaaa gtcgagctaa aatattcact
     7801 aagatgcgtt gttcgcgaca aattggcaga ttaggtcgca aaatatctac agagggatca
     7861 ataaaaaagt caatatattc atactctgtg aattcttttt gtacgtcttt cacagaagtt
     7921 gcaggaaaat tctctttaca tatttttaat agaaaatata atttatctat cataccgcta
     7981 tttagtcggt caagtacttc ttttttaaaa aaagtattca tcataaaagc cactacttgc
     8041 ggatcttcat cataaaattc tgtagcagta cttgaaaaga tgttccaata aatatctaat
     8101 gctgcatatt gaatttgtac ctcttttcca gaaaaagact ttctgccatt attagaaaag
     8161 gagatagaca aaccgtacaa tgctaaaaat gcattaatct ttttcatttt cgtatacaaa
     8221 taagaggcac tatagtttgt ttgctgactt aaactgataa tcgaatttgt ctgacagctg
     8281 aacaaagaag tcaagatctg ataactgact gattcgttgc agtatcttcc aaatagacga
     8341 taatagcagt aagccggatc aattccttca aaattcaact ttaaaaaagg tttttgctta
     8401 tcaatatgca gttctgttag attgagacgc tcaaagtctt gttctaattc tttgatgtat
     8461 agtaaaaaat ttgattcaga catatggaaa tgatctgcta aatattgtgt agtaataaag
     8521 aaacgatgtt cagataggaa aagaaaaata tctaattttg tattgctttt tttcttcaag
     8581 agaaacgttt taatcgcttc ttgagtttgc atgaaagagg ctccttttta cttaaattgt
     8641 taaaacttga aaatattact gtttttttca taaatttaga caaaaaaaca gtaaacatat
     8701 aggtagaatc tctactatct tatttcttgc atacaaaaaa atcaaggagt gagagagtta
     8761 tgagatattc aaccatctta atatcaacat ttgcagcctt aagcttatcg cttactataa
     8821 atcagacagt ccacacagag gagatagaaa cagccaaatg gacagaaacc aagtgggcca
     8881 acgtcaatcg aattaccttt caggatgaat tttcatcctt caattcagga gtcgcaaaat
     8941 taagaaaagg acaaacagcg accatcaaag caaaaattga ctattcagga gacttagcac
     9001 ctatactaca atcagctgtt acatttgaga atgttgatcc cgctttgtcg attggctatg
     9061 acgagaacac tttgactatc caacacaagc aagcgatatt taccttcacc gttacattaa
     9121 atcaggactt gttaaaacca gcgcttttta ctgtcaaggt atcagatgga ctaccgaacg
     9181 atcatcatca tttaacgccg tatagtcagc ggcagacaat tgagcaagat tcagctatcg
     9241 actcaggagg agatcttgtt gaagaaccaa cagataaacc agaaaatgaa aataagccag
     9301 aagtaccgcc tacagaaaat ccagacggag aacagaaacc ggaaatagaa cctggagaag
     9361 aacccgatac agaaactcaa ccagagccag acaatgaatc aaaacctgaa ataacccctg
     9421 gagaaaaacc agatgtagac ccagaagaaa aaccagatgt gactccagaa ccagatacgg
     9481 attctggaaa ccaaacagta ccggaaacaa atccagacac agataatgaa actgaaaatc
     9541 cagaaaaacc agaggtagcc ccagaagaaa aaccagatgt aactccagaa ccagatacgg
     9601 attctggaaa ccaaacagta ccggaaacaa atccagacac agataatgaa actgaaaatc
     9661 cagaaaaacc agaggtagat ccagaagaaa aaccagatgt gactccagaa ccagatacgg
     9721 acgcaagaga ccaaggaata ccagaaaaaa taaacaaaaa aacaattcaa gaggatggaa
     9781 aaaaagagag taaaaaatca aatctagcaa ttctaaaaat aaatgaagag cagctgaata
     9841 aaaaatcacg aatatttgat agcgcccaat cagctgaaac cttaaaaagt agcaaggata
     9901 caacctttgc tgtcctgaaa cgaaaaataa acagctacca aaatccggag agtcacaaaa
     9961 caaagttata ctgtggagcg gaatcattct attaagtata gcaacaatgc tttctgcaaa
    10021 aagatttaaa caaaacagat ctctctaaca tttgaataat gtctgtgaac gcatttttca
    10081 aataaaaaga aatatccaga aaatgacccc aaaataccag attgaaatct gatatttggg
    10141 gtcgttcata ctttatcttt cttcatattt attttaaggg atatctgttt cttttggaag
    10201 atggttcggg atctttttca ctttcaatac atctaaaaag aaggtaaaga tcggaatgcc
    10261 gacaatcaag ccccaaacgc caaagaaccg ttcgctaaca agtaagacca caaatgtata
    10321 aaaaatcggc agttctgttt tgctggacat caacttcgga ttcagcacat aagactcaat
    10381 tagatggaca atcaaaatcg taatcaggat ataaacaaca tctcggatgc ctccttggga
    10441 ataagctatg aaagatagag gaatacaaga aatgataaca cctgctactg ggatcagact
    10501 taaaatgaaa atcatgatcg ctaaactcag cagctgatga aagccaaata cagccaaggc
    10561 gattgtagtg agcaacgtat ttaaaagagc gacaacaaat tgagcttcta ataccaaacc
    10621 aaatgtattg acaaacttat cggcaaaata gtaaatatct tgaaagaacc aagaaaattc
    10681 actttttaaa aacaatcttg agaatacaat tgtttttttc ttttcaatca taaaaaagaa
    10741 acttaagatg aatgacatag caaacgccac tgccattttt cctatatcct gtaaatagcc
    10801 aagcgcaatc gaagcgccat tttgaagctg ttcaaaaaaa tcgtttcgct caatataagt
    10861 atggatatat tgtaaaacag gatctgcatc ttttggctga tgctcgtaaa aattaataac
    10921 ggactggatc atgtcaaatg tttgatgaat aagcatcggg atatactttg tgaatgccag
    10981 atagataagg taaacgatca gtgtataaat catgatagct aaaacacctg tagggattcg
    11041 gacaaatcgc tgaatgattt gaacaagatg aatcatcaaa taagtaaaga taaatgtgag
    11101 caaaatggtt gtaatcatac ttctaactaa gaaaagaaca aaaacgacaa ataataaaac
    11161 agtgagacgg cgtatttttt cattcgccaa aaaacgctga taaacggtca aatcgacaac
    11221 ctccaaataa caaaaaataa acacgttatt cattatatga tggaatatga aaataagaaa
    11281 gagcaaaaag aaaaaccgct aaatgacgtt atcttatgag ttggttttcc tcataaaatc
    11341 gttcatctag cagtttcagc cctttttctg gtcttgatga aaattatcta gcatttttcg
    11401 ggtcagatgt tgcagtgtat ccaattcttc ctgttcaagt ggaagataag acttgatctt
    11461 ttgtggaata ccttctgctt gtttttgcaa cgcagcacct tgagcagtca gcgtaacatt
    11521 tacgattctt tcatcacccg tttcacgctg acgattaata taattttttt ctgctaactt
    11581 ttttagtaag ggagtcagcg taccagaatc cagacacaaa tcatttccta gctctttcac
    11641 tgaagtcttt ttcttttccc ataaaactaa catcacaaga tactgggtat aggtaagatt
    11701 cagcggcttg aggaggggag tgtagtgtct aatgatttct ttagaggcgg catataaagg
    11761 aaaacatact tgattaacta aatatagatc ttccattttt ccacctccgc tgttttacta
    11821 tagtgtatca tagcggattt acacaaaaaa gtctattcac taactgcttt cagagtaact
    11881 tctatatttc cagctgtagc ttttgaatac ggacacactt tatgcgctgc ctctaataat
    11941 tcttgcgttt cttccagaga aagaccttcg atagatcctt caatctctac acctaagaca
    12001 aagctcgttt cactttctga ataaagggaa acttgtgcac tgacagtaga agccccagag
    12061 atattttttt gctgtttgat caattctaat gcactgttaa aacatgcact gaaaccagct
    12121 gcaaacaatt gttcagggtt ggttgcattt tcgacgcgtt taccaggagc tgcaatattc
    12181 aattcgaatg tacgatcggg attatgcact tctccagagc gtccgcctgt gttgatcatt
    12241 ttcgtagaat acattttttt cataaaatta ccacctttaa ttatatttta tacaattgaa
    12301 ttttacacaa tataattgtg gaaagcaaag aaatggattg gcataagata aataagagaa
    12361 agcacatgaa acagacgcgc tttctcgaat agcaaacata tacaaaaaat atcagagaca
    12421 aaaaataatc tcatcataaa cattacatat atttctcaaa aagttgtttt ctttcttcaa
    12481 aaattacttc acgatggacc tgatgaagca tccataaata accaaaagga tctctgaaca
    12541 ttgagttagt cacaccaaag tccatcattt ctgtcatttc ttgaatctct acacagccat
    12601 ttcgtaatgc tttattatgt gtgtcctcga tatcagagac gatgatattg aaccagatcg
    12661 tatttggatg ttcagcagta ggagcagtca aaccaagaga cgtgttttca tccagcatat
    12721 ggaatctcgt accatacaag gtaaaaatga cttcattatt gcctctaggg taatctgttg
    12781 cctcaatcag ttgtatttca tcaaagattg tttgatacag ttctagtgct ttcaaactat
    12841 catttaatac aaaatcgatt tccgttcctt tatgcataaa gatctcctct ccttttcagt
    12901 cgaacagtta ctagattctt ttattatacc agctaaagaa aacgcattca aaaaaatagc
    12961 ggatttactt accaactcat tcgattggat gataagtaaa tccgcgtccc attacttctg
    13021 ctacgctgct gacaatgaca aaggcacttg gatcgatttc ttctatcaat ggtttcattc
    13081 gggaaaattc atgatcagtc acaacgatca ataaaacctc ttttgacttt cgattatacc
    13141 cgccttttgc ctctagcagg gtaaggccac gtccaatctc agcacttaag cgaaggcgga
    13201 tgtcttccaa ataatgttca ctcatgacca taacagattt ctttcgattg atacctgttt
    13261 cgatataagt catgacaatt gaagtgattg caatagataa aatagcataa agaaactctt
    13321 ctattccaaa aataaacaga ttaagagaaa caacgaatgc gtctgtaaaa aataagccaa
    13381 tcgaagtatt caagtggaaa tattttttaa aaatgagggg agggatcgtc gtaccgccgc
    13441 tagaagagtt gtttttatat aaaatcgcaa ctcctaaagc aaaaatcgca cttccaaaaa
    13501 tgacagacaa caaccgatct gaagtaatca ttgtttctgg aactattgca atgattaaag
    13561 gaaaaaccaa gcttccgaaa agtactttaa aaaagggttt tttacctaaa aatagaagag
    13621 ctagtaccaa cataacgata ttcaacacta aaataactat agctcggtca aagcctaaag
    13681 cagcttccag taatattcct agaccgctga cacctccagc agcaatatga tgaggcccta
    13741 aaaacatatt gatagcgact cctaatatga agagggcaaa aataatctta atgtatgtcc
    13801 atataaattg tctcgtttct ttcatttatt cacttcct
//
LOCUS       DAHUVV010000070        13719 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.071, whole genome shotgun sequence.
ACCESSION   DAHUVV010000070 DAHUVV010000000
VERSION     DAHUVV010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.071"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            55..249
                     /locus_tag="NK767_002020"
     CDS             55..249
                     /locus_tag="NK767_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695008.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            518..817
                     /locus_tag="NK767_002021"
     CDS             518..817
                     /locus_tag="NK767_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695009.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(914..1348)
                     /locus_tag="NK767_002022"
     CDS             complement(914..1348)
                     /locus_tag="NK767_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6695010.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1580..2290)
                     /locus_tag="NK767_002023"
     CDS             complement(1580..2290)
                     /locus_tag="NK767_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCH6695011.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2280..3140)
                     /locus_tag="NK767_002024"
     CDS             complement(2280..3140)
                     /locus_tag="NK767_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCH6695012.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3145..3783)
                     /locus_tag="NK767_002025"
     CDS             complement(3145..3783)
                     /locus_tag="NK767_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCH6695013.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3798..4097)
                     /locus_tag="NK767_002026"
     CDS             complement(3798..4097)
                     /locus_tag="NK767_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6695014.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4140..5624)
                     /locus_tag="NK767_002027"
     CDS             complement(4140..5624)
                     /locus_tag="NK767_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCH6695015.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5644..6105)
                     /locus_tag="NK767_002028"
     CDS             complement(5644..6105)
                     /locus_tag="NK767_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCH6695016.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6123..7190)
                     /gene="ulaG"
                     /locus_tag="NK767_002029"
     CDS             complement(6123..7190)
                     /gene="ulaG"
                     /locus_tag="NK767_002029"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCH6695017.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7408..8178
                     /locus_tag="NK767_002030"
     CDS             7408..8178
                     /locus_tag="NK767_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCH6695018.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8451..9278)
                     /locus_tag="NK767_002031"
     CDS             complement(8451..9278)
                     /locus_tag="NK767_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6695019.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9846..11081
                     /locus_tag="NK767_002032"
     CDS             9846..11081
                     /locus_tag="NK767_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCH6695020.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12080..12340)
                     /locus_tag="NK767_002033"
     CDS             complement(12080..12340)
                     /locus_tag="NK767_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCH6695021.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12367..13668)
                     /locus_tag="NK767_002034"
     CDS             complement(12367..13668)
                     /locus_tag="NK767_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6695022.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4403 a         2820 c         2168 g         4328 t
ORIGIN      
        1 tcacttacat atatttgaat attaactaaa aacagcaaga aagaagagga tcaaatgtat
       61 tatgtagaag taaaaacaaa aggcgtcaaa aacaaacaat atgtgaaagg tatcagtaac
      121 gaatatcctt tacttggttc atggaaagaa gctgctcctt tttccaaacc atgtgccata
      181 aaaataaaga acgagctaga aaaagaactg acttgtggta aagctgttgt agatatcatc
      241 gaaaagtaaa tccaatgtat agaaaggaag atcacacatg aaaagcgacg tacaaattgc
      301 taagattata gaattagaag aagaagacgc agtgttgaca attgaaaaaa aataaatgtt
      361 tgtataccat atgattattt caatatgtat cctggaattg gagatctcgt agagctatat
      421 caagatgaat caaatatact tgtcattcat catgaaatat agagacttat aaaattgttt
      481 atttttataa tgtatcagta tagaaaggaa ataaaacatg gattatactg ttgaatttga
      541 cagtgaacta cttaagcaat ttccacaaga agaacatttc atgtataaag aagcaatttc
      601 ccacaaagtg acagagtatt tggagcataa tttttttcgt ataaagccag tgcgtactcg
      661 ttcggcaaaa ggaatatacg agatgaaagt caaaattaat aaaaaaaatt atcgcatagc
      721 atttgcttta aaaaaaaatc atgtgttcat tttttatatt agttcgaatt tgcaaaaaat
      781 tgtatttgat aaagaggtga gtaaaaaaat aaaatagctg agcattggag gatagattga
      841 gtgatactaa gtctatcctc ttgtttttct aaagaactaa gctagaattt tttcttttgt
      901 acgttattat atgttatttg tcattaccaa aaagcacctt ttttcttcta gtatctatga
      961 cataagaaat accacccatt tcctttactt gaacaaatcc tactgattca taaaattttt
     1021 ttgttttctc agtatcatct gtcaataatt cgatttggac tggtccataa ctagtgatct
     1081 tctccaaagc ttcattcaaa agcactttgc ctattccttg gcgctgatac ctcggatgaa
     1141 tgaggatatc ttgaataaat gcgatcgtcg caaaatcagt gatccaacga acgattccga
     1201 taatcttccc ttccccatta cgctttatca gagattcaga ttgttcaaat gctctagcta
     1261 accttgcagt gtcttttgtg taattcgtcc aaccaacgct cttatataat tcagaaatct
     1321 catcgatcat aagggaatca atattcattt ttttcttcct ttcttataca attctccctc
     1381 taataataca gttctttgac tatttaatct atcacatata actctcctgt aaagattctc
     1441 tcgattcctt aaaattcctc tttcctttta gaatgcatac tttttgataa ataaaaaggt
     1501 tgtgataaat tttcttcaca acctctgttt ctctctatca agtgttatct tttaattttc
     1561 tacccttacc ctagcatctc tattgcccgt agtatgcata ctttccatgt ttgcgaaaat
     1621 aatgtttatc tagtaaatat tgcgggattt cacatatatt ggggttgatc agctcgtttt
     1681 ctttggccat cagacaaatt tcatctaaga tcaaactgtt ttctactgct ttcataggtg
     1741 aatcccccca tgtgaacggt ccatgtccat agacaagtac tccagggact tcgtttggat
     1801 ctaattttct ttccttaaac gtctcgacaa tcacattccc tgtatgaact tcgtaagctt
     1861 ctcttacttc ttctttcgtt aattggcgtg tacaaggaac cttaccataa aaagcatcag
     1921 catgagtcgt gccataagct ggcaaatcgc gtcctgcttg cgcccacatg acggagtgcg
     1981 tggaatgagt atgagtaata gcagttatat cttcaaaagt ttgatacaaa acaacatgtg
     2041 tggggagatc tgaagatggt tttaagctat cttcttctat tatatttccc gacaaatctg
     2101 ttaccaccat ttgatctgct tgcatgcatt catatctgac accgcttggc ttaatgacga
     2161 tgactcctag ctcacggtcc acttcactca cattgcccca agtcaatttg actaatccgt
     2221 atcgcggcaa tgccagattt gcttcaagga cacgttgctt cattgctgca attttttctt
     2281 cacaagacat catagccagc ctccttcaat ttagaactta agtagtcttt tgcctgctgg
     2341 atttctcttc gaaagtcttg gcttttttca ctccacattt caatcagaaa tgttccatca
     2401 taatctagac gtttcaaatg tcttaataat ccaagaaaat caacacaacc gctaccaaaa
     2461 ggaacatcac gaaactggcc tgaatattct tttgttacag ggtaagtatc ttttaagtgg
     2521 atagcagtga tataatcgat ccctttctca agttctcgag ccggattatt ttccggccat
     2581 gcggacaaat tgcctaggtc tggatatact tgtagaaaag gtgaatgaat ctgttctttg
     2641 atttctaaaa attttgaaat ggagttcata aatggatcat ccatgatttc cacggaaaga
     2701 atgatgccat atttagaagc ttcctttacg ctttctttta ttccttcgat aaagtcttct
     2761 ctggaattca ttgatttctc ttcataataa acatcgtatc cagctatttg gattactttg
     2821 acgcttagat tatgcgccaa atggattgct tttttcatta gttgtttcgc tgcttttttc
     2881 ttttctggat cagcagaacc aaacggaaat cgccgatgcg cacttaaaca aatcgaatgg
     2941 atacgtacac cggagctgaa catctcttct ctcagctcag ctatcttttc ttcagaccaa
     3001 tctaatcgtg ctaaccgctc atccgtctca tcaatcgaca tctcgataaa atcgaagtcc
     3061 atttctttta caagggaaaa acgttctttc catgaaatat ctttgggtaa cgctttttca
     3121 taaatcccaa tttgagccat ttccttaccc ccagatacga atgatctcat ctttgaagtt
     3181 ttgtgcggcc ttcatcggat catttgctgc tgtaatacca cgaccagtga taaaggtata
     3241 cacatcgatc ccttcaaata agtgtaatgt gtgtggattt aatccaccag ttacagaaac
     3301 attaaagcct agttcaatca gttttttgat cttcgataaa tctttttcac cccaagtttc
     3361 accagataac agcgcatcac ggctttgatg gtagattacc tgccggatac cgatgttatg
     3421 ccaatccatg gcttgttcaa aagtccaatt gccgtagagt tctacttgca attcacctat
     3481 ttctttttgt gctgcctcca tagttggaat cgttgcacaa caaatgactg tcatccagtc
     3541 tgctcctgct tttgcaacat tactcgccac tgttcctcca gcgtctgcac atttcgtatc
     3601 agcgactagt tttttatctg ggaacatact gcgaatacag cgaatcgctt tttgtccttc
     3661 ctgtaagcaa agaattgtgc ccacttcaat aatatctact acatcaccaa ctttcataac
     3721 atctcctaat gcatgttcta agtcattgtg atctaacgca atttgcaaat tcggcctgct
     3781 catcttagtt ccttccttta cttgtttttt tggtaccact ttgtttcagc aaatttttca
     3841 ctgatttctt tgactgacat cacatttttg ataccgataa ctgtaactcc ttttttctct
     3901 gcctgagtaa acatattcaa aaaattgact ggcgtaaaaa ctacatcgta ttggccagct
     3961 gaactttttc cctctgaaat ggcacaatga tggattttgg taattggtac gtttagttct
     4021 ttcattgctt tttccacact tctcatcatc atcaagcttg ttcctgatcc atttgcacac
     4081 gatactaaaa ttctcatctt aatccctcca ttatgagtgt tgtctaatca gattttcttt
     4141 tatactctct gcttttctgc atatgtgtca tagtcttcta caatcaaaaa ataaccatca
     4201 ggatttttcc gatattgtaa ctgcggaata gccaataaac caatgacaac tacggcaatt
     4261 ccagcataac ctaggacttt catgattact gtaaacaatg gccaagctgt tgcccagtcg
     4321 aacatcccga tataaccacc gtatgttgac atcccaatcc atgtagcgaa taatgctgaa
     4381 ccgcctactt gtatgattcc tgaaagaaac gggaacaggc aagcggcttt gaatccacct
     4441 cggttattgg cataaactgc tataacggca ttatcaaaga acaacggaat gaaaccggct
     4501 atgacgatcg ttggtgattt caataaaatc agtaaaccaa tcatcaaaaa ctgtcccaaa
     4561 gcaccaaaca agaacccaat cgttaccgca tttggcgaac caaagccaaa agtagcagct
     4621 acgtcaattc ctggaacagc tcctggtaaa atcgtgtttg agattccttg gaaagattga
     4681 gttagttcat cgacgaatgt ccgaacacct aattgcaaga tcgccagata taccgcaaaa
     4741 tttaaagaag tcgtcataat gtagaataag aaactctgcc cttcttgcat gaattgcgct
     4801 tgaatcaaat aatctttgcc tagtacaatc agaatgatcc caaagaaaaa tagcatgagt
     4861 aacgaagtag caaccatatt ctcattgaaa atcgacaaga atcctggtaa tttgatatct
     4921 tcaatttttt tgtcttgctt gattgatcta tttttctttt catcccgctt tttcatccac
     4981 tcagctaatt ttgcaaatat aaagattccg aacatttgct gatgtgccac tgcaaatccg
     5041 gcaccttctg tcaattcctg cgtgattcct actgttaagt tagaagctac tgtccaataa
     5101 caacctaaaa tcagtcccat aaaaatcaat gcttgtatcc tacctagatc aggaaagcaa
     5161 aacagtaaaa tccaaaaagc ggtagctgct tgttgaacct gaacgtttcc ggtagtgaaa
     5221 acggaacgca gtttcgtata tttctgaaaa cggactaata agatatttaa aacaaatgca
     5281 atcagtaata aaatcatcac atctccaaag gtacggccaa atgtctccat taatccagca
     5341 tccacagcat tttgtccaaa ataaggatct gtaaccatgg catccaaatt gaaccgttcc
     5401 tttagtccaa ccaagattgg ccgaaaatta ttgaccaaac caccagagcc aacagtcaaa
     5461 ataaagtaac cgaccgttgc ctttaagaat ccagcgatgc attcatgaag cgggcgtttc
     5521 agcaaaatat agcctaataa cacgataaaa ccaatcaggt atgctggctg cgtcaagata
     5581 tttgttgcaa aatacgtcca aatgcttaac aaaaattccc ccatcttttt tctcctctat
     5641 caattaatta tattctgcca tcacaacttg gtaatcatcg atggagttcg tagagatgag
     5701 acgctcaacc aatccatctg tcattaataa ttcggataat ttttggatat tttctagatg
     5761 ttcttctgga tcttttgctg cgagtgtaaa aaacaaacta gccgttttct cctcattctc
     5821 cccttcactt tcaaaacgga ttggctgttt caacttggta aatgaaatag ctgttccaaa
     5881 aacaccatca cttttttctg aagaatgcgg cattgctact tgaggtgcga tcacaatata
     5941 aggaccgtat ttttctacac aatggatgat ctcctccaca tacgtttcat caataatttc
     6001 cttatcgatc aatacaagac agctttcggc gatagcttca cgccaatccg tgatttcttt
     6061 atctgggtaa tgaatcaatt gatggtcaaa aaagtatttc agcaagaaac tccccttctt
     6121 tcttataaaa acgacggaaa cggtgtatca ttttcaatcg agaagacatc ttcaaatcct
     6181 cgataaaaat taaactcaag atcatctttg tttgtcggat agatgaattt cccgcctact
     6241 tgccagatat aaggtttaaa tccatacttc aggcgatcct ttttgaattt ccaaatttca
     6301 gtgatctctt ttggatcagc cataaaatta gaccaaatat cgtagtggac aggaatcact
     6361 acttttgcat tcaatgactc cgccatgcgc agcatatcaa cagaagtaac tttgtctgtt
     6421 atccctcgtg ggttttcacc ataggcccct aaacagacat cgattttgtg ttcattacca
     6481 tgtttggcaa ataaattgga gtaatgtgaa tctcctgcat gataaagatt ccctcctgat
     6541 gtttcgaata aatagtttac tgcaatttga tccatctcct gaggcatttt tccttttaat
     6601 acctcttctg gatcttcaca agtgaccaac gctgtccgat caaacgcttc taaagctact
     6661 acattgatgt ctttgatttt gactgcatca cctggcttta ctgtaatcgt tttttcttca
     6721 ggaattcccc atttttgcca cgtgcgaacc acttcttttg gcccaatgaa tgttgcttca
     6781 ggacaatttt gatgaactgc tgcggctgta tggatatcta gatgatcgga atggatatgt
     6841 gtaacgatta aagcgtctac attttttatc gcaaaaggat cgattacgaa aggctgagta
     6901 cgtaaattag gctgcatatt ttgaacgcca ctcatacgca tcatctgatg gccttttttc
     6961 atcatgccat tcccatgact acgttttccc gtcccacacc atagatcaca taaaatattc
     7021 gttccttcat gacttttcag ccaaattccc gtacagccca gccaccacat agcaaaagtt
     7081 ccaggatcca ctacttctgt ttcgatttct tcattcaagt atgtgcccca ctcaggaaat
     7141 gtttctacta accacttttc ttttgttact tcattgacag ttttactcat ttgcttgctc
     7201 cccccatatc gttgttccaa cttacaagta aatattaatg tgtatcagca tccatgaaaa
     7261 cccttttata tgaccgtttg ttttttatat gattttcttt cagtaatcaa tgattgtttc
     7321 agtataataa agacagatat gaatgtttaa atgaacaact agtcaaataa atccatggat
     7381 caagatgtcc ctcaggaggc aaaagtcatg aaaaactcac ttgtcaatat tgaaaaacgt
     7441 caaaaagaaa ttttggaact gcttcagcag actgaacaac tatcaacttt cgaattagct
     7501 gaatcccttg atgtatctat tagtacgata cgaagagatt taaatttatt agaagaaaaa
     7561 aacgagatca tacgcaatta cggttattgt tcttataatt acaaaaacca gactgatttt
     7621 gatcaatctg gtcctgaaag gattaaacaa gcgattgcca gagaagctag taaatacatt
     7681 aacgattatg atacgctgtt tatcaattct agctcgactg cgttgaaaac actggatttt
     7741 cttcaatcta accatttgac agtcattaca aacaacgtga aaatcaatta ctctccgcac
     7801 aaagataatt gtagttatat tttaactggt ggagagatgc gctttccaaa agaagcacta
     7861 gtgggcgaca tcgcattaaa tacgatttct tcagtcaatg cagacatttg tatcattggc
     7921 tgtagcggag tcgatgtagt acatggggtc acaacaaaga tattaaatga atcaacgatc
     7981 aacgaaatga tggtcaacaa gacaatcaaa tgtaagattt tagtcgctga tcatcgaaaa
     8041 atcggactga catcaaagtt caagattgct gatatttcag tctttgatta tttgatcact
     8101 gataaatttt gttctgaaaa agtattagag gagatcagaa atacggggat aaaaaccatt
     8161 caagtagatt cattttaaga accaggacgt aaatatgtaa ataggattat caaaaaaatg
     8221 aataacaaat ttatcgataa atgaaaaaca cgctacgctc aaattgataa aggaatccca
     8281 agatgtatcc atccaatcga taccatcata atctattgct tcttttataa ccgttatatg
     8341 ttgcctaaat aggaatcaaa caatatcgaa aaaaggaggt atgacaaaag tcacacctcc
     8401 ttttttcgaa taaacggtat tcaaatcaga acactgattt cacaaccact ttatttcagg
     8461 tcgttttcaa tcacctttac cgcgtaatca tttccgttga aacgcgctat tttttttgcg
     8521 tccatatatc tttctaaata atcatcttct cctaattgct tatggcaaga agcaatttca
     8581 taaagcaagt attccaatgc ataagtcgat ttatgtttct tattatactc aatacccttc
     8641 tcacataatt ggattgcttt atcataatcc tcgattttac ggtaataggt ggcgatatca
     8701 taatatacaa tgcagaccat cagattatca aaatcagtct cactcaatat tttaagtgta
     8761 cgctccacat aaactttcgc aaaatctaat tctccttttg acaagtagat cattgccacg
     8821 tttgcagctg cttgtaagtg atggatatct gtttcggtcg tttgttccaa cacttgatta
     8881 aaataaaata atgctgcatc tggttttttc gcataaagat gatcaagcag tccttgataa
     8941 aataaaattt gatttaaaag atcagaatta cttacttttt cctgatcgat cgtttgtaaa
     9001 attttataag attcttggta ttttgataca gaattcaatg attgaatctc atttattttt
     9061 ttcctgattt tgtcttcttc tgtttctact aatatatcgg agatctcgac attcagcttt
     9121 ttgcaaagcg cacttaagat attgatgcct ggcgacatat tcttgttttc gatattgctg
     9181 acagttgctt gttcacaaat gccttctgct aattgtactt gtgttaaccc ctttttcttt
     9241 cggtattctt ttagtaatgc tccatctact ttaatcaaaa caatttcctt ccctttaaaa
     9301 aattatttct cctttatatc atacacaaaa aaaagttcta aacaaccaaa aatgtttaga
     9361 acagaataaa aattttaatt ttaaaataat attcaaatta aacaactggc gtatctgctc
     9421 cttgatttct ttttgtgtta tctgaataat tatcgtttac agttataaca catagtgaaa
     9481 attgtcatgg cgagaaagtc taagaatatt tttaaacata actatttctt tcttacgtgt
     9541 gtataatgac gacagcacac ctatcaatta atataattat tttatttttt tatgttctca
     9601 tgtttatttt taatgacaca acttctcgta tttatctggc acaacatata aatcgatcaa
     9661 aaaattgttg cgtgtttttt tgaaaattgg agtttcaact tgattacaaa atttaaattt
     9721 agaagttgtg aaaacagcga gagacagttt aatattcaaa atatacatag gctactcgaa
     9781 cgctgtgttt tttagtatac tagtaagaaa cgaagggata gactcattcc aataaggagg
     9841 atttcatgaa gacaattctt tttgctcctg cagtattcaa tttagctgag acaacacgaa
     9901 tgattgaggt tgccaaagct atgaaagacc gttattactg tgtattcttt ggcttttcta
     9961 aaacttttgc tcctttgatc acagaaagtg gctttgagta tcatttacta gcgccgatcc
    10021 ttacgaaaaa acaagaagaa caaatcataa aatttgacca gttgcgtaca cttaaaaacc
    10081 cttttaccaa agaaatggtt gcctctcgta tcactagtga acagagtctg attgctaagt
    10141 ttgagcctcg actgattgtg acaggttcga atgtgacaat ttttttatcg gcccgcaata
    10201 aaaagattcc tttagtatat gttaaaccct atgctttgag tgaacctttt tttcaatcag
    10261 agcatgcgct gatgccccac gagttacgtt tcttcggccc tttaagcaat cccctttggc
    10321 actggacaaa aaagcgtata gtaaataccc gatggatccc caatgccttc aaacacgctg
    10381 cacaaaaaga aatgcttcct ttaccaaaaa caagtttaca aatgatggat gctgatctaa
    10441 atctcatcac aacgcctaaa ctatttttgg aagaagaaca gctgccggaa aattatcgaa
    10501 tcgttggtcc aatttttgct aagctcagta cttccttgcc tgaagaagtg ctaactttta
    10561 tacagcgaaa gcaacaagaa agaaaacgta ttatctattt cgcaatgggt agttcaggca
    10621 atccgcgtat gttacgcaaa attttgttct tcttacaaaa acaaccggat ctagcggtta
    10681 ttgcacctat tgactatctg cttgatgaca ttcaatctga ctcagaagat cttttaataa
    10741 cagaatatct gtctagtcat cttttgcatg aattcattga tttttctttt atccacggcg
    10801 gggaagggac cgtccaaact gcctgtgaat ccggcaaacc gtttatcggt gtggggatgc
    10861 aatttgagca aagctgtaat attgactatt gtgtaaaata cggaaacgca atcgccctca
    10921 caaaaccagt gactgaaaga aaactgcaga ttgctctgcg cgaagtttct tcacccaaga
    10981 ttcgtcagca ggcacgcacg ctgcaagccg tttttccaat aaatggtcca caacaagcaa
    11041 ttcaagaaat cgaaaccttc ctgagcaacc acccaatata gtttcaagta gaaaaagctt
    11101 ttttaataga gactcatcag taatttcgct attcgagacc cagacagtga aaatacaaaa
    11161 aaagtaaata gtggattgaa aagttagagt tacttaaatt acggtgtcta aaaaacacaa
    11221 aatcatcctt aaaataaaag atctgacgct atccaaatga gcataggtag cgtctgccat
    11281 ttattcttag tcaaattctt tgacaccttt ctctggttgg atcatctcaa cgtttcttca
    11341 aaagaaagac cataataatt aggcaaatac attttcttaa aatgtgtcat atagatctag
    11401 aagcatcgct ttgtagagat ttgtgccgct agtcccctca acaattacta aatcaaatgt
    11461 aggatttacc actttatctt tgataatcaa cctccctctt ctgttgagac aatgatctcg
    11521 caatccgatg catactccta tataaactgc cagcaatccc cacatattga gtagatcatg
    11581 tcagaatgtt ctatgtcgtc agcaacgata cactaaaagt cacgttcacc atgactgatt
    11641 gctattccca gcacaattat ttctatacac gtttcatgat ttgaataaac actcacatca
    11701 tacatattac cattcataca ccatactgca gcccctacta tatggttgta agctcccaaa
    11761 tctatatttg acttgatcac tttggtagta gatctactaa ttctaaatcc attgctgata
    11821 aaatttccat tttttcctcc ttctttctgc caaaacagtt aaaacaataa taatctttat
    11881 gtaaaaaagg gctgtgacaa aagccttgtc acagtcccat attccgaata aacggtggtc
    11941 aaaagcttgc atctgcggta ataagttcaa ccaaacgaaa aataagccaa catttcacaa
    12001 aaatttaaga agcaattttc gtaaatttct tcttatttct tgaagctaac cacttctgtt
    12061 acaaccactt ttagaacatt taaagcttcg atgtgacaaa ccatctatgc tgctcgatca
    12121 taaaattgat agcaaaaatt ccacaaagta catatgtccc ccatgaaaac aaaacattca
    12181 tatgcggtac aatgatcttt ggaagaatca cgatcaaaaa tgatgccagt gcccaaatcc
    12241 atcgattgat ttgaatatga tatgtagcta gcatattcaa tataaggtga ctagccgtta
    12301 ataaaaagat aaaaacaaaa aataaaatca gccaatacat tgtttcccac cttctaaaaa
    12361 ccaaacttag acagtctgga cttcgttttc tccacgactt tgttcatctg acagcatctt
    12421 tttatcataa gcttttacaa atggataata gataataaat gctatcaatg tgttgatcat
    12481 gatcaagata acaacacgcc agtcatttcc tgctgaaaga taaccgccga ttggagctgg
    12541 taatgtccat gttggcaaaa cagtaaatct attgaccatt tctaatttga ctgcaaaata
    12601 actgataata gtcaaaacta aaggaactaa gttgaacggg atgaacatgt aaggattcaa
    12661 catcatagga gctccaaaaa ttatgggctc attgatattg aagataccag gaataataga
    12721 aaaacgacca acctctttca agaattttga tttacagaag aatataaaca aaacagatac
    12781 cacgatcgtt gctcctgcac caccaattgt taccgtccat tgataaaact gttcaggagc
    12841 gatatagggt aattgggtgg cagctgtacc atctgccaat gcctttgcat tctcatccaa
    12901 catgatcagc cacattggac gaacaacagc ccctacaata ctcatgccat ggataccaaa
    12961 tgaccacaac aaatggatca aaaacattgg tagtaaaaca ccaagcaaat tattccctaa
    13021 aatattggtt aaaggctcga ataacgataa taaggcagcg tttacatcaa agcccgcgac
    13081 gcttcgaatc agccaagtaa ttgttaaaat gactgcacct ggaatcaatg cctcaaaact
    13141 acgggcaaca gcaggtggca cttgttctgg cattttgata gtaatatttc gatttttaaa
    13201 gaaacgatat acttccactg caaaaatcat agataagatc cccgcaaaca tcccagatgc
    13261 tcccaaagaa ctcataggta agacccagcc aagaggtgcc tcttcaggag ttaaaacgtt
    13321 tacaggaact tgtaacataa agaacgttcc taaagataat ataccacctg tcacactatc
    13381 taatccatat gatttagcta gatccgaacc cataacaaaa gttgcataaa tagccatcaa
    13441 tcccatcgtc atcctaaagg gtaaaacaat cgttgccgaa taaggggcaa taaattcatt
    13501 ccatccagga aacggtaaat tgataaagac cataaaaaat gtcccaatca aaatcaatga
    13561 caatgttgaa acaaccccat tacgaattgc taataaatga cgctgtgtac caatttttgc
    13621 cattggtggg acaagtttct cttcgaccca tttcatcagc ttttccacta aaaccactcc
    13681 ttttcaataa aatatgaacc attcattgca tgacaacgc
//
LOCUS       DAHUVV010000071        13526 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.072, whole genome shotgun sequence.
ACCESSION   DAHUVV010000071 DAHUVV010000000
VERSION     DAHUVV010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.072"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(334..894)
                     /locus_tag="NK767_002035"
     CDS             complement(334..894)
                     /locus_tag="NK767_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCH6695023.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            1036..1650
                     /locus_tag="NK767_002036"
     CDS             1036..1650
                     /locus_tag="NK767_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6695024.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            1839..3719
                     /locus_tag="NK767_002037"
     CDS             1839..3719
                     /locus_tag="NK767_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCH6695025.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            3798..4025
                     /locus_tag="NK767_002038"
     CDS             3798..4025
                     /locus_tag="NK767_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCH6695026.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            4030..4572
                     /locus_tag="NK767_002039"
     CDS             4030..4572
                     /locus_tag="NK767_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCH6695027.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            4799..5515
                     /locus_tag="NK767_002040"
     CDS             4799..5515
                     /locus_tag="NK767_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HCH6695028.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(5575..7593)
                     /locus_tag="NK767_002041"
     CDS             complement(5575..7593)
                     /locus_tag="NK767_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCH6695029.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7606..8604)
                     /locus_tag="NK767_002042"
     CDS             complement(7606..8604)
                     /locus_tag="NK767_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695030.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            8802..10043
                     /gene="sstT"
                     /locus_tag="NK767_002043"
     CDS             8802..10043
                     /gene="sstT"
                     /locus_tag="NK767_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCH6695031.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            10190..10825
                     /locus_tag="NK767_002044"
     CDS             10190..10825
                     /locus_tag="NK767_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCH6695032.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            11016..11756
                     /gene="truA"
                     /locus_tag="NK767_002045"
     CDS             11016..11756
                     /gene="truA"
                     /locus_tag="NK767_002045"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCH6695033.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(11901..13499)
                     /locus_tag="NK767_002046"
     CDS             complement(11901..13499)
                     /locus_tag="NK767_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695034.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAF
                     IFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYT
                     MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAEN
                     YNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTP
                     IYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTL
                     ILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         4530 a         2135 c         2633 g         4228 t
ORIGIN      
        1 tgattcccct tgaaaatgaa ttgaggtaaa cgagaagaat tcaataaact ggatgataaa
       61 aatataatat gactataaat tggttacagc ttcaacctat aagttgtaat aaattagcct
      121 aaaagtaatt tattaatgat gaggtgaatt tgattggtac aacaagatat atcacttaga
      181 gagggagaag cagcactaca aactactttt caataagtag gaattataga aattttactc
      241 aaaaaatctt cagtaaacaa tttggctaaa taagattcag ttgttatacg agaaggttac
      301 ggttcttctt aactaagctt agtatgtttt tattcaaggc atgattttct ctatgtgatc
      361 attcagtttc tgtaagagtt ctaataattc ttttttggag tacttgtaat tgctttctac
      421 ccaccgattg agaacggata gacaaccttg gacgtggtac gtagtaaaga atctcatttc
      481 ttctgtgata atcgttttct tgtcttcata tagccatatg ttcagatagt tttcttcaat
      541 aacttgttta actttcttct gaaacgaaat atcaccatgg ttgaggataa ataagcgata
      601 taaaaaagta tttgcgtaaa catagtcaat caagcgagtg tatagttcct catagctatg
      661 ggctggatca taagagagaa tttcatttag atcccgaaaa aagttgcttt ctaattgatc
      721 atatagatca tagatatcct tataatgtgt ataaaaagtc gaacgatgta catctgccgt
      781 ttctgttagt tcgcgtatag tgattgcttg aatcttttta tttactaata atgtggatag
      841 tgtttcagcc aaagcttttt ctgttttttt gacgcgtcta tctgtctttt tcatttacct
      901 atctccttat ccgacatatc aaagcaaagc tgtcgcttaa tcgacaaact tatagatgat
      961 tgattataga tagtagacac tgtgtctatt ataatgtaag tataacacaa tcattgaagt
     1021 tgaggaggcg aaatgatgaa actaactttt aaacccttag aaaagaaaga ttacaaaaaa
     1081 gctatctatt ttgctgttga aggaatgaac tttgagagat atatggaaaa tgaacttctg
     1141 ctgaatcttt acggaagata ttttttgtat atggagttga atcgagcaac tcaaattctt
     1201 gctttgtatg cagaggacga gttggcaggt gttttgctgg cggagataaa aggagaaaaa
     1261 aagaaacatc gttcttttgg acagcaaata tatgttaaga tattcgattt tttacaaaat
     1321 atatttgcta aagaaagtaa aggagcttac gagacagcaa atgaacaaat gcttgcagca
     1381 tatttaaaga agcattcgcc agatggagag atcatttttt tagctgcaaa tcctgatttg
     1441 aaaacaaaag gcatcggtac caagttgcta acagaatttg aaaaaagaga gaaaggaaaa
     1501 gaaatattcc tttttacaga tagtggctgt acgtatcaat tctatgaacg acgtggattt
     1561 gaaagactaa aggaaaaaat tagcaagata aaaatcttag ataaagaaat agatctccga
     1621 tgtatgctgt acaggaaaat cattcaatga aaatttcgat agaaacgaca atagttggca
     1681 agatcacttt tagtgagtct tcttgctaac tattttttgt agtgtattct accaaaagaa
     1741 aaccgccttt ttgactaaac ttgatagtgg tcaatttttt atgatggata agtatctata
     1801 cttagtctcg taagaatgaa aggaaaagag ggagaaagat gaaattacaa aagtggatcc
     1861 aagagcatcg aaatcatatc accgccttat cagctgtgtt gattattttt ggttttatcg
     1921 gaaaatttac tggttttgtc ttgttctata acattgctct agccatagct tctattattg
     1981 cggtcatacc tattatgatc catgcgtatc aggcagtgaa agtcaaagtt atctcaattg
     2041 atttactcgt tactattgct gtgcttggtg cctttatgat cggagaatac aaggagtcag
     2101 ctatcgttac attcttgttt ttgtttggta gctatctgga acaaaaaaca ttgaccaaaa
     2161 cgcgggagtc aatcaaggct ttgaccgaga tggccccatc tactgctagt gttgtccaag
     2221 aagatggaac gactgaagaa gtggaaatcg atgacgtgga agaaggcgat atcgtcttag
     2281 taaaaactgg tggagctatt cctgtggacg gacgagtcat tgacgggaaa gggtatgcgg
     2341 atgaagccgc agtgactggt gaatcacgac aagtcgtcaa aatcagtgga gatgaagtct
     2401 tctctggaac gattttagca gatggctacg tgacgattga agcaactaag gtcggagatg
     2461 atacgacctt tgccaaaatt atcgaattag tagaagaagc acaagatacc aaatcccatg
     2521 cggaaaaatt cattgatcga ttttctcagt attatactcc aaccgtttta cttattgcgt
     2581 tggttgttgg tctgattagt cgtgattttc gtttagcaat cactgttttg gttcttggtt
     2641 gtcctggcgc tttagtcatt ggtgcgcctg tttcgaatgt agcaggaatc ggtaatggag
     2701 cgaaacgggg catcttagtc aaaggcggtg aagcaatgga tactttttcc aaaatcgata
     2761 cttttgtctt tgataaaact ggtacattga ccaaaggaac tactgctgta tccactatca
     2821 agaactatac agaggatgaa ggggacagct tagctttagt ggctcgatta gaaacgttat
     2881 ccgatcatcc tctggggcgc gccgtgatcc attatgctca ggagaaagac attgattttc
     2941 aacaattagc agtcaaagat aatcagacaa tcaagggaca aggaatcaca gctgagattg
     3001 atggtcatac agtttgtgcg ggcaacacca aattgattgc cgctcacaaa ctttctctca
     3061 caccaaagca agtacaagat ttacatcaat tacaacaaac aggcagctcg gtcattattg
     3121 tagcaatcga caaaaaaatc actcaaatca taggtgtgtc agatgtgatt cgaccagaag
     3181 tcgctgaaca attagctaag ttgaaacaag caggagcaaa acagttagtg atgctgacag
     3241 gtgataatca gatgactgct gattatgtag ctgatatgtt aggcattgat gaagtccatg
     3301 ctgaattatt accagacgaa aaggttcaat ttgtgaaaaa ataccaagaa caaggattgc
     3361 gcgttgcttt tattggagat ggaatcaatg attctccctc tttggctgcg gctgatatcg
     3421 ggattgctat gggatcagga acggatgttg ctatcgaaac gagtgacgtt gtcttgatgc
     3481 aatctaattt tgaatcgttg gttcatgcct accgcttggc taagaaaact gttttaaata
     3541 cccgtgaaaa tgtagtgatt gccattggtg tcgtcttatt tttactcaac ggcttatttg
     3601 ccgggtttat ttacatggct agtgggatgt tcgttcacga agctagtatc ttgatcgtta
     3661 tctttaacgc gatgcgttta ttgtattttg gacacgaaag caaagaagaa aaatcgtaaa
     3721 acttgataag aatcaatttt tttatggaag ctgtaatcta gaataagact atcaagaaaa
     3781 aggaaggaag aactacgatg aaaaaaatta ttatgcaatt agatacacta gcttgtccaa
     3841 gctgtatgca aaaaatcgaa catgcggttt ctcaacagcc tggggtaagc aatacgaaag
     3901 ttttgtttaa tgcgagcaaa atcaaagcaa actttgatcc agatgaagtc caccctgaac
     3961 aattagctgc agttgtggaa aaattaggtt atgaagtcca atcagcaaaa gtaaaagagg
     4021 cgtaaatcaa tgaatatagt ggaagaaaaa tttgttcaag aaacaaaaca agcagatatc
     4081 gaccatcata aaccaactgc tggcgccatg gtggggcatg tattagcaaa ttttaaatta
     4141 gaagaaatga aattgatcca agcacaattg tatgttttgg gtacatcttc gcaagaatta
     4201 agtacccttt tttcagaata tgctgatcaa gaagcgagat ggttcaataa aatctctcat
     4261 gagttagtga ttgaaaatga aatcgttccg tctactttag aagaagtcat gcgttatgga
     4321 aaaatttacg aacatggtca aaacaaatat ttaccagcct caacaatgtt ggaaaacttt
     4381 gcaaaagatt ttgatttcca aaatgtcttt atcacacggg caatcaaatt agctgaaaaa
     4441 gaagaaaaat atggcttgca acaattgctt gttgaacttt taagtttcaa taaagaaatc
     4501 atctaccaag tacaaagact ccaaggaaaa actgtccgac aagatttgga tgaggaagac
     4561 gatgatgact aaaagtctat gtttcttcaa atacaaatat aaagcgtgag gtaaattgga
     4621 aaatcacgaa gattaaaata aaagaagcac ccgctgatgt attacagaca acatcagcgg
     4681 gtgcttctca gtttttagaa gatttgaaag attgatgaga aaatacgaaa aatcaataaa
     4741 atcacattgc ttatttgata gaataaagaa gtgggagtaa tcgaaaggag ttttttaatt
     4801 gaaaaaaagg attttagtaa caattggtct agcttttttg ttagctggtt gtaccaacgg
     4861 gaatgaatcg aaaaaagaat caacaaaaga aacagcgaaa gcaacagttg caactactac
     4921 aacaactagc acagagagca agtcaactgc agcaagtaca gaaacagcag atacctctac
     4981 ttcgtcagag tcttccaaaa cgactgaaac aactcaaaca tctacttcaa gttcttctgt
     5041 agaggcttca tcttcctctt cagaaagcag cgaacaagtt gaacaaacac tgtggaatgc
     5101 agacaaatcc gcacaattag aggcatttat gaccaaatgg ggccaaacaa tgggacaaca
     5161 atacaaaagt tatacgaatc aaatgagtgt ggacttatat ggtcttaaag tccctcaagc
     5221 tattttgaac ggggagtgga agatggctat tggtggagtg cctgtttctg cagaatggtc
     5281 agaaagtggg acaggtcaag cagactatca aatcacagcc gtctattccg atgcagagac
     5341 agaaccatat ctgaaaaagc acgtctatct attcgggttt cagcaaaatc agccaaaagt
     5401 attggtgaca caacaaaatc aggggaatcc cgataattat ctttacttca acgagacagc
     5461 gaataatgaa ttgaaaaatg gctttaatca aatcgtttat ggacaagcaa attaatgaga
     5521 taaacaaggg gttgtgacat gtcttttatc acaacccctt gctcagttta tttgttagtc
     5581 ttttattttt tgtgtcgccc atgttgcact gtactggtta cctgcaacaa gatcatattg
     5641 tatcagacga taatcttcta gaatttcttt ttgtttctca gttaaatttt tttcttggat
     5701 aatttgacta tcctctgtaa caaagacctg ttttccatta aaacgattag acaagctttc
     5761 tgtaggatca attgttaatg caggtagatg attagatact tctgtcaaca acgcatagta
     5821 aggagttact ttgatattcg catgctcaag agccatagca ccaaagttat taggagcaac
     5881 aacttcttta tttaacgtag ctgcttgttc acgactatac ttgttgttat aaatgaaata
     5941 atctgtctgg tgctgttcta atccatattt tttcgaatca ttcccactat agattgcggg
     6001 taaatggtca ccataaaaaa caaaggtaat cggtttatcg attttgtcga tttcttggat
     6061 gaattttttt actgctgtat cggtatagtg aaggccttgc atataagttt gtaattcttt
     6121 tcttctatta ggaatgacag cagttccttc agcagtgtag ttcaattcac tatagtaatc
     6181 atcataaggc atatggtttt gcatagtgga taattggatg aattgtgacg tttccgtatt
     6241 gctttgtaaa gctttcaagg tttctttgta tgctgaatca tccgatatgc gtgggctttg
     6301 ttctatttta tccgtataag ttaatttatc cggactgtct aaataataga acctgtcaaa
     6361 accaaattta tcaaacactt gttttctgcg ataaagagca gctgtaaaag gatggatggc
     6421 tattttttca tcgaatagat cggtaatatt tggacttatt ttctgacgct ccactagctg
     6481 ggtataaggt gtaactaacg aagcagacag gttactgata ctcaagcctg tcagtccttc
     6541 ccattccatg ttagctgttc cgccaccata accgacggat aacatcaatc ctcctgttcc
     6601 ttctgttata atgcttttga tgtaaggaat agggtctgta ggtactgtca gattagggac
     6661 acgtgcagga tcactgaagc tttcacttaa attgagaacg atcgtctgat tgtttaacca
     6721 gtcacttctt gtttggttga tttcatctgc ctttgtgttg tactttttca ttattccatt
     6781 aattttttct ttcgaatagc cagcaggttc ttccatgatt ttaatgtcga tattgtttag
     6841 gaattgaatg actggtccgt tatcttttac ggcgccttgc tggttaaaga aagtcttgtt
     6901 tactttaaat aaattaaaca gcaagaaaga aggcgaattt ttgtgattaa tgaaaaagac
     6961 acccgaaaaa catcctaata atacagcgat actgataaat cgacgtttcc aatgaatctt
     7021 caaggaatac tgtttttgta gtctacgttg tacaataata ctagtaatca ataataggag
     7081 aaggatcaat ccgcccacga ttaataagac tgggctaacc atcgataata gttctgacaa
     7141 tccggtcagc atttttaaat ctgaaggaaa gacaggctct tcccgcattt ccattttgat
     7201 tactgtcgaa atagtgagca gtaagttaat gatcaaggta gcagtcaagg cgtaccaaaa
     7261 acgattggaa atcaacaata gtaagaagaa aaatcccata ataatgatga ttgtcaggat
     7321 catgggtgct tgtcgctgag taaatatttt taaggcaacc tggatagttt ctttccaacc
     7381 ttcttttggt tctaaaacga aaatctgcac gaaggtaaag cagtaaaaaa atgctgcaac
     7441 taaaaaataa cttttatgaa caaaaagcca atgaattctt tttttccaac gctgagttaa
     7501 ttgttcaatc gaatcaaaag cgaagtattc gatgaaacga tgaatgaaac ggattttttg
     7561 tatataaaat aaaaagataa ctaggaggga cactgctaat aaatatcaaa taaaaaactg
     7621 cccgatattt atcagccatg ccttccctga gtttggaaat tcacgtttca taaatgtaga
     7681 tacgcgataa gaaactaaag ggaaattaca tataacttga gtgacaatca aaaatacagc
     7741 aagaaaattt cctcttcctt ttatacctaa tttatcagat agcatttcta aaaataaaaa
     7801 cgaagagaaa gtataataca ttgcgaagag agaatatggt ttagaaaacc tcagcgctgt
     7861 atctgtattt cctctgatga tgagtgaaat ttgtgtcatc aaaataatca aaccgaataa
     7921 aacaatcaag ctgataaata cttgaggaag cttatagcgg accttttctt tccagttcca
     7981 ttctttcaaa ccataaccca ttaaaaataa ataaaaaatc cataaaatac tctgtccatt
     8041 ctgtaatgca aacgggtctt tattaaatag agtagagcag ccaactaatc caattgttaa
     8101 aaataatagt agatatttaa ttgttgtttt cgataattta tttaaaaatc caactgtata
     8161 aggaatacta caaaaaagca aagtacaaga aacagcataa gtaaagtaat tctgactgat
     8221 aggtgttagg ataagccaat aatcacgaat attcaagctg ccagcttgta cgtaaaaaaa
     8281 gatattgcaa actagtgtgg ctattaggaa tataaaggca aaacgactta aataatgtaa
     8341 aacttttaaa cgggcgattt tttgatattg gtaacccatg taaagtggga tcaaatttgc
     8401 accaataaca acggaagttt gtaaaaatgt atagatgtag aatcgaccag ggtttgttgc
     8461 atatgtagga atagtgactg ctgaaatcaa tatacgctgt aaaaccatca ttatacaagc
     8521 gacaaagatt gtcaggaagc cataactctc cttctttatt tttttcaaga ataaacacct
     8581 cacaaaaaaa ttggacaaaa acataagaaa acgtttcctg ataagttgcc cattaataac
     8641 atcctaactt agaatatctc aaaaaacaac gtgtagacag aaaaagtaca aaattagcaa
     8701 gaaatggttg tttgtattgt tagaattttt agaaaattat acctttagtt tctttgcata
     8761 aaatgataca atgattgtta aaattgtgaa agggtggtca tttgttagag aagtttttgg
     8821 atatgtcgtt agttcaacgt attgttgttg gtattttagt cggaatattg tttggtatct
     8881 ttttccccac atggacattt atttcaatat tgggaacgtt gtttgtcagt tgcttgaagg
     8941 caatcgcacc tattctggtt tttttcttga cgatggcgtc aattgccaag cataaaattg
     9001 ggaataagac attcgtgaag cctattttga tcttatattt agtgggaaca ttgctttctg
     9061 cacttgtagc agttattgta agttcaattt ttactgttcc tattacttta caagaaacag
     9121 tatctgaaaa agcaccgcaa agtttggaaa gtgttttatc tactatgttg acgaatgtca
     9181 cacaaaatcc tgttcaatct cttattgatg cgaattacct tggcgtctta ttctgggctg
     9241 tattgttggg actagctttt cgagcacgtt cagaatcgac aaaagagttg gtggatcaga
     9301 tatctatagc gttgtcacaa gtcgtacaag tgattattgc atttgctccg atagggattt
     9361 taggattggt ttatcaatca attgctacga ctggaatcgc tggattggca gagtatctgc
     9421 agcttttact tgttttgatc ggtacgatgc tgtttgtcgc attagttgtg tacccattcc
     9481 tcacgttttt gtttattaaa gaaaatccct atccattgat tttcttttgt ttgaaagaaa
     9541 gcgcacttcc cgctttcttt acccgaagtt ctgccgcaaa tatcccgata aacatgatgt
     9601 tggctgaaag attgaaattg acaaaagagt cttattccat atcgatcccg ttaggtgcta
     9661 cgatcaatat gggcggtgcc gcgattacca tttccttgat gacgttgact gctgtacatt
     9721 ctttagggat tgaagcacct tttgttttga aagtcctttt atgtattttg tctgctttag
     9781 ctgcttgtgg agcttcaggt gttgcgggcg gttcactgtt attgattcca ttagcatgta
     9841 gtttgtttgg tatctctaat gatgtcgcga tgcaaatcgt cggtattgga tttattatcg
     9901 gtgttatcca ggattcaatg gaaactgcct taaattcgtc aagtgatctg ttgtttactg
     9961 caataggaga gctaggcgcg cgtaaaagaa gtggggaaaa aatcaaattg aaagattgtt
    10021 tagatggatt aagtgaacgt tgaacgttag atatttagta tttttaaaat gattcgagtt
    10081 tttaatgagt tatatagatg ttcgtgtaaa tagaggtttc tgttaaatca agataaagtc
    10141 gtttataata acaattaaaa gcataggtaa ttgttattgg aggcgtatca tggatttttt
    10201 acgtaaattt ggttttttct ttttattatt aggcgtgctg agtgattttt tgactccgta
    10261 cgtattagga ttgtattatc ctgaattaga tcaaatgaag agcgtgatca gtgtatttgg
    10321 agaggtttcc agtcctgtaa gaaaagcgtt tcttatttgg tctgtggttt cagggatctt
    10381 tttcatcctg tcattgcctg cattgtatgc tgcatttgtt agtacatctc gtgtgttagc
    10441 taccctagtt acagcagcca tcgcgttcta tggtataggt gattgtatct ttacaggttt
    10501 gttcagtatc aatacccatg aatcttcgtg gaatgtctcg acatgggtcc ataatatcgg
    10561 atcaggttta ggttacagtg gttttttatt gtttccagtt cttcttttct tgatctatcg
    10621 aaaaaatggg caaatggacg taagccactt ataccttgga ctgtctgtga tcagtttgct
    10681 ttttgcggcg atttatggac tggcaagaat tccttctata aattcttggc cattactgaa
    10741 taaaataggt ttttgccaaa gaatcagttt tttctttaat tatgtgccca ttgcctggtt
    10801 gagtatcttg catctcaaac agtaaaactt gagccgtcaa attgaacagt cagcggatac
    10861 ttcatccggt ggctgttttt tgtttttctg aaatatatat ttccacagtt ttcttgtata
    10921 gcttctgtct tagagaagca aggagctata ttttgtgcta caatcttaaa aaggtagaga
    10981 gaagaccaaa tggaagacag aggagccact aaaatatgag aaacatcaag ttgacaatcg
    11041 aatatgacgg aaaaagatat tcaggatggc aaagacttgg agatgacgat aaaaccattc
    11101 aaggaaaaat cgaaaaagta ttgcatcaga tgacaaatga agagatagaa attattggat
    11161 caggaagaac agatgctggc acccatgcgc gaggacaagt agcaaatttt aaaacaaaca
    11221 cagaaattgg actggcagaa atgatcgact ttatgaatcg ttaccttccg cgggatattg
    11281 tcatcaaaag aatcgaagaa atgcctgagc gcttccattc aagatataat gcggtgggca
    11341 aaaaatacag ctattatgtt tggaacaacg tgattccttc agcatttgag cgaaaccaca
    11401 gcttttattt ccctcaagag ttggatatgg acaagctgaa tgcagcttgc gaaaaattag
    11461 tgggtacaca tgatttcatt ggtttttctg cattgaaaaa atcaaaaaaa tccacagtgc
    11521 gtacgattga tgaattatct atcgaacgaa agggagaaat gcttcatttt acttttgtcg
    11581 gagacggatt cttatataaa atggtgcgga ttatcatggg gacattacta gaaatcggca
    11641 caggtgccat gccgatagaa acaatcgatg aaatatttga aagcaaagtg cggcaacatg
    11701 caggagaaac cgtccctgca caaggtctgt ttttggatga agtttattat cgataaaaat
    11761 actttgataa tggatgaaac gcatgtttca cagtataaat atcgatgtta aataatgtat
    11821 gaagcaaatt aaagaaatat aaaaaaagac tttttagcct gtttatgtgt acagctaaaa
    11881 agtctttctt aaataaaaat ctagtgagaa tgttgtactt ctcttctcgg ttgtttttgt
    11941 ttagaaaaag taaatcgata aagtaaaaat aaaatcaaag taacgataga taaatatctt
    12001 gaagcaattg ctagttttgt gtactgataa gtaataacga ctttatttat acctggagga
    12061 atagttaatt cagttgtgcc aaacttagat aattttgttt gtactagttt gccattaata
    12121 gaagccactt gccctttata acgataaata ggtgtcgtca agacagtatt tttattattc
    12181 gaattatcta attcaattgt aagtttgtta ctcataaaat gagttgttgg atttatttcc
    12241 tggttaccta gtaaatattg atcattatta atcatatcag gatgatagat tgactctttg
    12301 tttgcataat cagtatgatt gtaattttca gctatagcaa cagcgtcttc aggtgctata
    12361 agtgttaatg aatctttgtt agcatggata gaattgctta ctcctgagag atgcaatatt
    12421 agtgcaagaa acgtaaagac cattattttc catgaattat ttttagtaga aaaaatataa
    12481 tagatcacta tactcatcgt ataggctata aataaagaag aaaaaccatt taaccgccag
    12541 acaaattgta gactcttaac tggtgtatgg ttaaaaagtt gccaaggaaa taaattggtt
    12601 gaacaaaaaa gaacaaatac accaagaaag aaaataaata aatcatcagc agttaatttt
    12661 tttataaaaa taaaagcacc aatcaatccg agaaataaga ataatccaat agtatagttg
    12721 ttatagttat tatttaaaat atgtgtcagc atatcactag gagccatacc acttaattct
    12781 tttcccagtg ggactttcaa ttcttgtgct ctagattgtt gaataaaagg aattaagaat
    12841 cccgcactta aaaaaaaggt gaggacggta gcttttaaaa gacttaagag tctagcgatt
    12901 ttttggtccc aaaaataaaa agaaagaaac aacgttcctc caataaatac agaaaccata
    12961 gcgacagaaa gtaaatgagt atacactact agcgtcatgc caatagacaa ccagtaccat
    13021 ttttggtaat ctctaatata aatttcgtaa caccccatta gtatcaaagg caaaaatgtt
    13081 aaagcgacag cttctcccaa tgaagcacga tggaatatgt caatggcacg ataggcggaa
    13141 aatgtataaa taatcgaaaa taacagcgaa atataacgat tgttttttat ttgtttcata
    13201 gaaaaataag aaacgaccat tgttaaaaaa gtgataaaaa aatagtagat attataactt
    13261 aaaacaagat ttcccatcat tttataaaat aaaaaagctg gataaagagt aagccatgga
    13321 taaaaaatat tcatcattgt tccatgatga tcgaagttat tgaagtttac tggagaactc
    13381 catacattat ctagtcctat gattcgagat aaatgaaagt aagtatcctg attgacacta
    13441 tgatagattc ccttagacat gaatagaggg atgacgtaag caaaacttag aaataacata
    13501 aaaatgatat ctattatttg gttttt
//
LOCUS       DAHUVV010000072        13279 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.074, whole genome shotgun sequence.
ACCESSION   DAHUVV010000072 DAHUVV010000000
VERSION     DAHUVV010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.074"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(307..1440)
                     /locus_tag="NK767_002047"
     CDS             complement(307..1440)
                     /locus_tag="NK767_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCH6695035.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(1431..2099)
                     /locus_tag="NK767_002048"
     CDS             complement(1431..2099)
                     /locus_tag="NK767_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6695036.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(2555..3541)
                     /gene="lacD"
                     /locus_tag="NK767_002049"
     CDS             complement(2555..3541)
                     /gene="lacD"
                     /locus_tag="NK767_002049"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCH6695037.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(3554..4480)
                     /gene="lacC"
                     /locus_tag="NK767_002050"
     CDS             complement(3554..4480)
                     /gene="lacC"
                     /locus_tag="NK767_002050"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HCH6695038.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(4493..5008)
                     /gene="lacB"
                     /locus_tag="NK767_002051"
     CDS             complement(4493..5008)
                     /gene="lacB"
                     /locus_tag="NK767_002051"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HCH6695039.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5027..5455)
                     /gene="lacA"
                     /locus_tag="NK767_002052"
     CDS             complement(5027..5455)
                     /gene="lacA"
                     /locus_tag="NK767_002052"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HCH6695040.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(5475..6248)
                     /locus_tag="NK767_002053"
     CDS             complement(5475..6248)
                     /locus_tag="NK767_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCH6695041.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            6413..6892
                     /locus_tag="NK767_002054"
     CDS             6413..6892
                     /locus_tag="NK767_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCH6695042.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            6949..7251
                     /locus_tag="NK767_002055"
     CDS             6949..7251
                     /locus_tag="NK767_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCH6695043.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7273..8730
                     /locus_tag="NK767_002056"
     CDS             7273..8730
                     /locus_tag="NK767_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCH6695044.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(9323..10075)
                     /locus_tag="NK767_002057"
     CDS             complement(9323..10075)
                     /locus_tag="NK767_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCH6695045.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(10290..10526)
                     /locus_tag="NK767_002058"
                     /pseudo
     CDS             complement(10290..10526)
                     /locus_tag="NK767_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(10536..11500)
                     /locus_tag="NK767_002059"
                     /pseudo
     CDS             complement(10536..11500)
                     /locus_tag="NK767_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(11706..13279)
                     /locus_tag="NK767_002060"
                     /pseudo
     CDS             complement(11706..13279)
                     /locus_tag="NK767_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
BASE COUNT         3877 a         2868 c         2251 g         4283 t
ORIGIN      
        1 acggttgtgt aaaaatatca ttttagagca tgaaaaaaag agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacatt tttgatcctt tttatttgat taattatatc
      121 cagctggata ataattttgg gggcaacatt tctatcttac tattatcttc ctaaagtagt
      181 agactttctt attgggaaaa attacaacaa aaaggagtgg cttagttgac aactcctttt
      241 tgtttacaaa tcaattggat atctataaat tgtattttct aacgaatttt atagacatct
      301 gatttgtcaa attaagctag acaatatgtc tttatctatg cagctcaaaa atacttccaa
      361 tggtgttcga taatttaatg attttcttgg aatatggttt cttttcgagg caacggattg
      421 aataaaggtt tcatccgtgt ccctaaagtc cattgtttta ggtaagccat cctgcttgag
      481 cccatgcact cggtactgat gagaaaaaat tgatgcctgt ttctcctgag gaatgccaat
      541 tccctgatct tttacttcaa taatcacgct acccttttgg tttgaaatgg tgacaattac
      601 cagttttcct tcaccatatt tgattgcatt gtcaactaaa ttcgaaaccg cccgataaat
      661 ccaacgatct cgtccatgaa tcagggttgg ttgatcttca gtaaacgcaa acaaaagatt
      721 cggataattc attttgtagg catcacaaac ttccgcacag atcagtgaaa cgtctacgac
      781 ttccgtgcta agatcctctg agttattgga taatgtcaaa atatcgtcaa tactgtcggt
      841 catccgatct aaaagcatta aatttccttg atcggcatgt tgccgttcta tttccagatt
      901 cgtacgcaaa atagcgatgg catttttctg ctcgtgggtc agataactat ttaatcgtcg
      961 ataatcctcc aaatgaccat cgaacttatc ctttagctta ttaaaagccg catttaatgc
     1021 ctcgtccttc acagtatttt taggaacttg atcaataaac gttaaatcac gcgtaatcgc
     1081 taagatttga ttactgtaaa ttttgcgtaa aaccaaccaa agaataaacg ttaataacat
     1141 gacggcagaa caaataacca gcaccatcgc tggtaaatat ttaaatagtt cgtcctggta
     1201 agctttatct atcatcgaaa tagtcataaa ttgattgttg gaagatttcg aagcttccat
     1261 ctgcgtctct gcttgaaccg tcttaccctc ttccataaaa taaacatctt cacctaaggt
     1321 agatgcgata ctgattccct ccaaattatt tctcagaaag aagaaactca ctaaaatcac
     1381 gcctgtcacc aacagtgaaa aagccagcat cgttaacgtc attttaaaat ttaatagcat
     1441 agctggtacc cctttgctcg aatggttttt aagacctctt tttcagctgc ttcagccagt
     1501 ttctttttta gacgagccag atgcactcgc agaaccgacg aaaaaggatc aaaatcttca
     1561 tcgtagacat gttcagaaat ttcctcagac gaaacaaccg ctggatgacg ctgagcgatg
     1621 cacagtaaga tatcaaattc cttgggtttc aggaccacct ctgtcttttg atagctgacc
     1681 cttcgaccta atggatcaat cttcaaagca ccaattgaaa tctgcggcag cgcacgtcca
     1741 tgataacgac gaataaccgc gcgtattcgt gcttttaatt ctagtaactc aaagggtttg
     1801 actaaataat catctgctcc aaaatccaag ccttttgctc gttcttcaat ctcatcacgt
     1861 gcagtaataa tgataactgg actttcgact tcttcagcac ggagactttg taaaacagat
     1921 aatccatctt ttttaggcag atttaaatct agtagtatcc catcgtatga attgatgaag
     1981 gccttctctt ctcctgactc gccatcataa gctacatcta cagaaatatt ctcctgctct
     2041 aggccctttt ttacggataa aaccagctca cgattgtcct caaccaccag tacacgcatg
     2101 tatattcact cctcatacgg atttagtaat tacacttatg ctttttagca tagtacacgc
     2161 tcttcttcaa agtccacgtt ctttttgtga agatttgaag aaattcatac attctgttaa
     2221 aaaacacata attaagaatg attttactgt ttgaaaatac ctagaataga aaaagactgg
     2281 aacctttcct ccagtctcac tactttctat ttgcttggtt ttcttgtata tacttttcaa
     2341 agttcgttgg tttatgacca ataagcctcg ttagaatact gaaaattgcc atcaagtcat
     2401 taagtaccac atgtaagaaa atctctctaa agaaaaaccc cttcgtttta actcacttac
     2461 tattttagaa ttcgttcccc taataatgca gcatgatttt acgctttatg tataaaaaaa
     2521 gactgcaagc aattttgctt cacagtctga tttcttatga tagtgagctt gttaacactt
     2581 ccaccggtgt agcagtttct ttcaatacgg catttaattc ttcaatattt ttacgccctt
     2641 gcgtattcat ccactccatc gctgcttctt ttccatcaac gatatagctt tctacaccat
     2701 ctgcccaagt cgcacggccg caaagtacac cattgaaggt tgagccggca tttttcgcga
     2761 agcgcagtgt ttcttggaac aactctgctt ttacaccggc gcttaggaaa ataaatggca
     2821 ggttagtggc ttcagcctgt tccttgaaga agttagccgc ttgtgccttt gtgtacacga
     2881 cttcgtctgt cccatagcct tccacataat tcatattgac gggtacttcc acctttaaca
     2941 catctacgcc ataacgtgga tcagaaaatt ccttcatcat atcgttgact ttatgaggtt
     3001 tcttaatggc gtattctttc gaagtcgcat cagagatgtt cgcgtcataa gaaacaagct
     3061 ccaagaagaa gggtaagttt tcagcctgac attctgagcc gattcgttca ataaaggcat
     3121 gtttttgatt attgatttct tctgcttcat cgacatcata gtaaagtagg aacttacaag
     3181 catcggcgcc tgcttctttt aaacgtttaa ctgaccagat cgataataaa tctggtaaac
     3241 ggccgggagt tgacgcatcg taaccagttt tttcataagc taacagcaag ccggcatttt
     3301 ttgctctaac gtcagctgct ggcaagccat attcgggatc taataaaatc gatgaggcat
     3361 atttcgttaa ttgtgaagaa acaatttctt taaattcaac gatttctgat tcttctgctt
     3421 cgctgccctt gaattttgtg atcatcttct tcaaagcccc gcgttgatca attgctaagg
     3481 ccccaatcac tccatcttca ttgcatagtt gcttgatata ttgacgtttt tcttcgttca
     3541 tttccgtatc ctcctacact ttctccacta agatttgatc atatagtttt tgataattgg
     3601 ctaattggac atagccagtc gtcgcttcct gagcattcag cattcctaaa acattaccct
     3661 gttttaacac ggcttcatcc gaaagacctt gttgaatcgc agcggtcaat ccggcaacgg
     3721 tcgcatcacc agagccaact ggattgacga catcaatctt tggaattgtc acgcaataaa
     3781 aagtctcgtg atgtttggca aaggctccgt cgccccccat tgaaaccact atccattcaa
     3841 tttcatcaaa catcgaatct gacaaggcgg cttttaaatc tgataattct gttgagattt
     3901 ctcgtcctag cagaccagcc agctcttcgg tattgggttt tattagctta ggctttacac
     3961 tgcctcgcaa cacttccctt aatgctgtac ctgaacaatc taaaacgaca ggctttggct
     4021 gacaaacttg cagcatttgt cgataaaaat cagcaggcaa tccttctggc aagcttcctg
     4081 aaaaagaaat gatttcggct ttaacagcta atttttcgaa atgttgcaag aacgcccttg
     4141 cttcttgttc agtaataaca ggtccgtttt ctaggatttc cgtctgtttt ccgtcatgta
     4201 aaacagcgat gcaattacgt gtctctccgc tgataggtaa aaagtcatga gcaatctcat
     4261 tttgagtcag gtccttttta atctcttcac ctaaaaaacc gccgattaaa ccactagcaa
     4321 tcacctccgt atctaattgc ttcaagatac gtgtcacatt taggccctta ccgccagctg
     4381 ttttgcgaac atttcttact cgattgactg tgtcgagctt gaaggtttct aatggataag
     4441 aaatatcaat cgatggattc atcgttattg ttaaaatcat ttgtccaccc gcttaatcgt
     4501 ggtattcgcc acgatcccat ttctcgataa attcatcgaa gaaatgcgga tcagactgct
     4561 tgtcactggc tttttcgacg tgatcgattt tggcgatcaa ttttttgttt tcttcagttt
     4621 cttgataatc ggcattgatg aaggcctcta atatgtcaca gatcaaaaat tctcccgtaa
     4681 tccgagcgcc aaagccgatc acattggcat ttagttgttc cttcgcatag atagcagtcg
     4741 tcatatcgcg aaccagcgca ctgcggatac caggtacttt attgacagcg ttgttaatcc
     4801 caacacctgt tccacacaaa caaactccta gatctgcttc tccgctagtc acggcctctc
     4861 cgactttttt tccgtagatc ggataatggg ttctggtaaa gtcataggtc cccatatcca
     4921 acacttcatg tccttttgat tttaagaaat ctgatactgc catttttgta tctgtgacga
     4981 tgtggtcaca gccaattgcg attttcattt cattcatcct tcctaattac gccattttat
     5041 ttaacatgtc gacacgtact tgatgacgac caccatcata atttgcttgt agaaattcct
     5101 taacaatatt caacgccaac tcatcgccaa caatttttga tccaagtgtg atcatccgag
     5161 aattattatg tttacgggtc atatacgcac tgcgctcatc cgaaacttct gctgctacca
     5221 ttcctttaac ctttgttgcc gtgataaagc tgccagcgcc gtagccatca accgcgattc
     5281 ctaaactacc ttcgttttca ttcagatcat ttgcgatcgc caaagttgat tccacaaaat
     5341 cttctgaagc cgtttcactc ttatctacta ctgtaatacc ttctttgatc aattttttct
     5401 tgattaattc ctttaattcc atgccgtcac tgtctgaacc aattactaca cgcatttttg
     5461 tttcctccta ttttttactg aatgatttta gtgaattgct gataataact cttcagctcg
     5521 gctgaaagag tagaatccgt aaataatttg tcgatcttgc gcaaatcata gaaattataa
     5581 aaatcgctat ggtcgaattt gtttgcatct gaaaccaaac atttttctgc cgcattatct
     5641 aaaacaattt tttgcaaggt accttcttca atactgaaat tcgaaacctc cgaacccttc
     5701 aatccattca ctccgacaaa agccttctta ataccgattt tttgaacagc ttcattggca
     5761 attgaaccga caaaggctcc agtcttttcc cgatatagcc caccgatcaa atacagttca
     5821 aatcttttta tttcacttaa tagattgaaa accggcagac tattggtaac gattcggaga
     5881 gatgacggct tgatgtatcg tgtcattaat tcgatcgttg tacccgatcc aagaaaaatc
     5941 gtgtcgtctt cttgattgat atgagctgcc gcatttttag cgatgtattc cttctctttt
     6001 gtatgcagct tctttttttc agtatgagaa agctcatttg cttctataga agtcgacatt
     6061 tccagataat cgattttttg tgcaccgcca tgaacgcgta aaagcttttt cttctgcgcg
     6121 agttcctcta gatctcggcg gatcgtcata tctgaaacat tcagtacttc ctgaataaat
     6181 ttgactgtta tcgtcccata gcgatcacac atcgctaaga tcgaacgctg tcgttctgtt
     6241 ttcagcatat tctgtacctc cacgacctta taataaaaca tttacgaaca tttttcaaca
     6301 catattgttc gtttattttc gtttatgcgt tgacaatgat tttgtaagcg gttatactca
     6361 acttgtaaac atataaataa acataaacga actaataaag gagctgtttt gtgtgaatga
     6421 attctttttc aaagacactg tctatgtttc tgacaagcag tcgcaggaag aagtgttcga
     6481 ggaagtctat caagatctgt taaagaaaga attggtaacc gaagattttt tggagaacct
     6541 tttagaacgg gagcgtaatt atccgacggg attagatctc tcacctgtct ctaaaatatt
     6601 gccagatatc gctatccctc ataccgaaag tgagtttgtg cgaaccaccc gtatcattcc
     6661 aatcaaagta aaaaatgaaa tccctttcta caacatgatc tcgccaagcg atcagatttc
     6721 cgcttcattt cttttcatga tcttgaacga aaatggtgaa gcacaggctg gcatgttggc
     6781 taatatcatg gatttcatta attctgttga cgaagaaaaa ctgttttcgt ttttcaaatt
     6841 agaggacaca gaagcgattt atcatttttt agaagaacat ttttatatat agaagtctct
     6901 gcctaaccgc aggataaaaa ataaacttat tttaagggga gaattattat gatcaaaatt
     6961 ttagcagcat gtggagcagg cgttaattca agccatcaaa tcaaaagcgc tttggaaact
     7021 gaattgaaaa atcgtggcta tcaagtcacc tgtgacgccg ttatgatcaa agatatcaat
     7081 gaagaaatgc tgtctcatta tgacattttc gcacaaattg ccaacaccga cttgggtttt
     7141 gacattaaca tccctgtcgt tgatgcaggt gctattcttt atcgtattcc tgctatggct
     7201 gagcctgttt accaaagtat ggaaagcgca atccaatcat taaacaaata ggactaaaga
     7261 aaggaaaagc atatgaatac aattattgat attgccaata aagtatttac ccccctaatt
     7321 aatttaggtg cggcaccgtt aatgaccatc gtattaactg ttattgcttt attttttaaa
     7381 gtaaaaccgt cacgcgctct tgaaggcggg ctcaaattag ggatcgccct gactgggatc
     7441 ggagctatta ttggaatttt aactactgct ttttcagacg ctatgacagc ttttgttgaa
     7501 cgcacaggta tttcattaaa cattacagat gtcggttggg ccccactagc aaccatcaca
     7561 tgggggtcgc catacacgct ttatttttta ctaattatgg tgatcgtcaa tattgtcatg
     7621 ttagcaatga aaaaaaccaa cacattagat gtggatattt tcgatatttg gcatttagca
     7681 attgttggtt tgttcgcgat cttctgcggt gctaatctgt ttattgcttc tgcattagtt
     7741 attttcattg gtgtattaaa aatttggaac tccgacttaa tgaaaccaac cttcaatgac
     7801 ttgttaaatg cgccagacag caacccgatg accacgactc atatgaacta catgatgaat
     7861 ccgattatta tggttttcga taaaattttc gacaaaatct tcccttggtt ggataaatat
     7921 gatttcgatg ctgccaaatt gaatagtaaa attggcttct ggggatcaaa attcgcgatt
     7981 ggaatttatt tggggatttt tgttggatta ctagcaggac aaacaccaac gcaaattttc
     8041 tcgctagcct ttaccgctgc cgtctgtttg gaattgttct ctttgatagg cgcttggttc
     8101 atcgcagccg tagaaccgtt atcacaaggg attaccgatt ttgccaataa acgactaaaa
     8161 ggacgtacca tcaacatagg tttagactgg cctttcatag caggccgcgc tgaaatctgg
     8221 gctgccgcta acgtattagc accaatcatg ttgctagaag ccatcatctt accaggaaat
     8281 aaattattgc cactaggcgg aatcatcgca atgggtgtta ccccagcact attggtcgta
     8341 actcgtggaa aattaatccg tatgatcgta atcggcgcga tcgaattacc attattctta
     8401 tggtccggca ccttgatcgc cccgttcgtt actcaaacag ctaaagcagt cggagccttt
     8461 ccagccggct tgaacgggtc cgccatgatc tctcatacaa cgatggaagg cccgattgaa
     8521 aaattcctag gttatttagt cggtaatgct tctcaaggac aaatcgaatt tgtgatatac
     8581 gctgccttag cattaggtgc atacctttta atcttcattt ggtacgctcg tcaaatgaaa
     8641 aaacgcaacg ctgcatacgc agctgaaaaa gaagagaatg catctccggc aacggcgcat
     8701 aatatgactt atgcagccga agctaaataa gctaaaaaca ccccgaatgt ctttagactt
     8761 cggggtatta ttttacctat aaaaaataaa tagatgtaaa atccatttca aaaagcatag
     8821 cccatctctt gccacaccca ataaagaaat tatcaacgtt tatgcttttt ttaactcctt
     8881 taagcttgcc tttgttggta ttcctcaacg attctttttg tgttcttgtt tgttttgtgc
     8941 tttttgactt tttgttgttt aattgcgaat tccttgattt cttttttcct gaatcgcttc
     9001 gcatcccgcg gttatgcgtg cgatttcatc attgcttttt ctgatctaac tcgtttcatg
     9061 tcaaagagct tcagctgact tagtgtccat ggttgttttg atatcccttt tcctccttaa
     9121 taaagcggtg attgatactg ctattttttc cttcacataa acataacatc ccaaaaaaat
     9181 cgactatctt ttttcaaatg gtgtattcaa gtattcggag gaattagtaa tgatgcaccc
     9241 taaaaataac ataattcgct taaattcaag gctaccaaaa aactaccaag ttctaattat
     9301 ctagagtttg gcagtttctc atttacacaa catatttttc ttctttttcg attttttcat
     9361 agtgcttttg ttcatcatca tccgttataa ctactgttac atcattcaga ttataatata
     9421 ctgaaaaatc tcttttccca attttcgaat gatcaatcaa taaaaagcgt tcaacagaat
     9481 tattcagagc gatctgttga gtttggccct cttcaaaagt cgaagtcata attttctgtt
     9541 catttaaagc attacagctg aaaaatgctt tatgaaaatt catatcctgt agcgatttat
     9601 tcgtgatttc accaacaaag cattgagtcg tttcccgcat ctcgccacct aatagataga
     9661 tttttcgatt cccttttcgc ttatttaatt cttcaaaaac aggcaaacag tttgtaacaa
     9721 tccgcagtga atccccttga atgatttttg acatgagctc gattgtagtt cctggtccaa
     9781 gaaatatcgt atcaccatca ttgatcattt cattcgcctt ttccacaatc cgctgctttt
     9841 cttcttgcag cataatctgt ttttctctat gagaaagctc cacatgccga taaaaatcct
     9901 tcaatttcgc tccgccatgg acgcgttcta ataatccttg tttctccatt tcgtccaaat
     9961 cacgtcggac tgtcatatcc gaaaccgaaa acttctccat aatatcgctg actcgtactg
    10021 taccgttaac ctctaataac gacatgatcg cttggaatcg atccgttttt aacatttcat
    10081 cgcctctttt ctttttaaga ctatcaaatt cttaagaaaa gtgctagaac aagttttctt
    10141 aagaagcctt ctgcaggaag gaaagcaaaa tttttcagaa tttgcaagct gctattctaa
    10201 aaaataaaac cgaacgagca tgactgtcat agtgacagcc ttgttcattc gacttttatc
    10261 tttaataaat cattttattt aatctgaagt tattgtgcaa gttcaaaaac aaagtagtta
    10321 atgtctcctc gatatttcga aatcgaatac tcaacgggca gacgttctag cgaaaaaccc
    10381 tgtgtatgga aatagaacat tggatctcct tcttccacat ctaacaaatc gctgatcgta
    10441 tcatccgctt tgattacttc tagttttctg gtgaccgttt taattggata acccatttct
    10501 tcaaaaactt gatacatttt ttcttttctg atttgtcaaa ttaagctaga caatatgtct
    10561 ttatctatgc agctcaaaaa tacttccaat ggtgttcgat aatttaatga ttttcttgga
    10621 atatggtttc ttttcgaggc aacggattga ataaaggttt catccatgtc cctaaagtcc
    10681 attgttttag gtaagccatc tcgtctaagc aagccgtttg agtgctcgtg ctcatttagc
    10741 cctctttgcg aaggtgttcc tggatctgcg aaaaagatgg caatatcgtt cgagttactt
    10801 atagatcgcc agttggaaaa ttcttttcca caatcaaatg tcatggattt aaacaagtgt
    10861 ttaggcatgg atttgaacca atcatttaac ctgttttcga tatcttcggc tcgtctgcct
    10921 gtcggtttca aagtaataat aaccttggat aaacactcaa ccaatgtaat aacagcgctc
    10981 ttgtgtttaa ctcccacaat ggtgtcgcct tcgagatgac caaattcttt agtcaggtgt
    11041 ggatagagac tgttccgttc atgaatcgtt ctgcgaaagg cctgttttcc acgcttttct
    11101 tgatgattat tcggtttacg tttccccttc ataggtaacg actgtgtgtc aaaaaggcct
    11161 tttttaaaca tccgatagag tgtacgaaca gaacaatcaa ttggaaactc gccacggccg
    11221 ataatcacat cgggtgacca cccttgagct accttttctt gaacatatgt tgattgttca
    11281 tcagataaaa cggttgaacg tcgtccacaa cgagatttat tttgtttgta ttgacggtaa
    11341 taatctagca cttttttcct tgtttgagaa acgaataaac attataaatg gtttgtctag
    11401 agcgatttaa actttgtgcg acaagagcga caggtttctc gatattgaaa taagactcta
    11461 tgattactag ttcgtctgtt gtaagatggg tgtaggtcat ttgtactcac tccttattaa
    11521 ttctttggtc ggaactattt tgagtgtagc acaaatgatc tttttagttg tctagcttaa
    11581 ttttacaatc gacgcctcaa aaagaatacc cgaaatttaa acagcaaaaa aagatagcgc
    11641 ttaccattgt gtttaagata aaacgctagc taagttggct agcgctttat taatctaagc
    11701 aagctctaaa attgcttcaa tacaaaatca actgctcctt taggatcacg tgttaaattg
    11761 atatattctg cacctttagt tgcaattact tttatcccta atttgtctgc atctttacgg
    11821 agatcatcca aataggaatt cacttgaggt gctagaacga ttaaatcaaa atttttcatc
    11881 atatcataat gagaaccata agctcctgct ttagcagaaa gattaacacc tgtctctttt
    11941 gcgccctctt ctaatgcatt agccaacatt gcactcgttc cggcaccagc acaaagtact
    12001 aaaacgcgct ttccttcacc aaccgatgct tttgcttcat caactataac tgccgcagtt
    12061 gcaccaactt cttggatttc ttcgaccaat tcttctttta cttcttctgc agcaatgttt
    12121 tttaactctt gttctactaa ttcagaatca taggccttaa caaaaggtaa ataaataatc
    12181 gcatcgacaa caatcaatac taccgctaat acaatcgcca acacactaaa cccggttcct
    12241 aaaaccaagc caatcggtgc gggcgtcgcc caaggcaata cggatataaa gctattcatc
    12301 gacaaaaagt ttgaatatcc atacgttagc gattggtgct aataaaaatg gaattagaaa
    12361 gtacggattc aaaataatag gagtagcaaa tagtaacggt tcatttaccg caaacgaaac
    12421 tggaataaat gaagctttac caactgcttt caattgtttt gatttagcga acaacatgaa
    12481 cagaaaagga acaactagtg tcgctccagt accacccatc gttccaacaa agtttcctag
    12541 accaactgtt aatacttttg aggcttgtgc accgttttta aataattcaa agttagtttc
    12601 tacgttcgca taaataattg cagcaatcgc tggttcaaca atcgatggtc catgaacacc
    12661 aacaaaccag aacattgcca tggctcccca aatgattgca ataccgacat atccatcagc
    12721 agcactaaat aatggctgca ataattgaat tacacctgct gcaaatgatg ttccaaaaaa
    12781 ttgacgaact cccgtatcaa tcaaaacggc aataaatact gcaattgaaa aggggataat
    12841 atctctaaac atctgtgtga ttgttccggg aacttctttt ggcatacgga tcgtcaaatc
    12901 tcttttaaca cagaatttat acacatttac agtaacaaac gcagaaacga aagctgctaa
    12961 taatcccttt gtccctaaat aatcagtagc tatcccacca tcaatttgat cagctgccat
    13021 cattacaaaa ccacaaattg acgctaacat aacagaaact tcattaatct gcttacctga
    13081 atcagataat tgacggttca ttgaattagc taaacatcga gctgttgtag caacaaccaa
    13141 aagaccaact aatcccattg aataaccata aactttccaa agccacgtcg agaatgcctc
    13201 tggtaacgtc acgttaaata cttctggaac agccgcaatc ataataaaaa tacttgaaaa
    13261 tagaatagct ggcatagca
//
LOCUS       DAHUVV010000073        13255 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.075, whole genome shotgun sequence.
ACCESSION   DAHUVV010000073 DAHUVV010000000
VERSION     DAHUVV010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.075"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(314..386)
                     /locus_tag="NK767_002061"
     tRNA            complement(314..386)
                     /locus_tag="NK767_002061"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(351..353),aa:Lys,seq:ctt)
     gene            767..1336
                     /locus_tag="NK767_002062"
     CDS             767..1336
                     /locus_tag="NK767_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HCH6695046.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1323..2183
                     /locus_tag="NK767_002063"
     CDS             1323..2183
                     /locus_tag="NK767_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCH6695047.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2373..3077
                     /locus_tag="NK767_002064"
     CDS             2373..3077
                     /locus_tag="NK767_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6695048.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3082..4917
                     /gene="walK"
                     /locus_tag="NK767_002065"
     CDS             3082..4917
                     /gene="walK"
                     /locus_tag="NK767_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCH6695049.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4914..6230
                     /locus_tag="NK767_002066"
     CDS             4914..6230
                     /locus_tag="NK767_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695050.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6231..7100
                     /locus_tag="NK767_002067"
     CDS             6231..7100
                     /locus_tag="NK767_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCH6695051.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7155..7964
                     /locus_tag="NK767_002068"
     CDS             7155..7964
                     /locus_tag="NK767_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCH6695052.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8067..8696)
                     /locus_tag="NK767_002069"
     CDS             complement(8067..8696)
                     /locus_tag="NK767_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCH6695053.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8866..10158
                     /locus_tag="NK767_002070"
     CDS             8866..10158
                     /locus_tag="NK767_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6695054.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10234..10896)
                     /locus_tag="NK767_002071"
     CDS             complement(10234..10896)
                     /locus_tag="NK767_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCH6695055.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11121..11405
                     /gene="groES"
                     /locus_tag="NK767_002072"
     CDS             11121..11405
                     /gene="groES"
                     /locus_tag="NK767_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCH6695056.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11456..13081
                     /gene="groL"
                     /locus_tag="NK767_002073"
     CDS             11456..13081
                     /gene="groL"
                     /locus_tag="NK767_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCH6695057.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4408 a         2285 c         2844 g         3718 t
ORIGIN      
        1 agcaagtaaa tttagttttt ctattaaata ttcttcactt tttaaccttt tatagtactt
       61 agatttaatc ctatatgttt agagagttca ttggcagcat gattttgcaa agcttctcga
      121 attattgtta tattagaaac tttactgttc ataattatgc ttctttttta actcaaagtc
      181 caaagggcta cagtggactt tgagttaaaa ataaaaagcc ctagttattt tgaaaaataa
      241 aaaaagaaca gcgtaaatgt tttcgaaatg ctttcgcacc aacatttacc tgttccgtgg
      301 gtttggcacc caatgggccg tgaggggctc gaacccgcga cccgctgatt aagagtcagc
      361 tgctctacca actgagctaa cggcccgtac aaaaagtact taataatagt agcactaaga
      421 attttaaaat gcaagactac atttaaagaa aatcagaaaa atctttactt ccttatagga
      481 aatcattcat agattattca catttgctcg ctattatata agcataccaa caaacaaagt
      541 tcttgcaaga gaacaaaccc taacactttt ttcatttttt actcctttcc cgccgtctcc
      601 ccggcgggtc tcctgttctt aacgataaaa gacaagaagg cttagccttc ttgttttttg
      661 ttttcatgga atcccgctaa tcaaatcacc tagttaaagc aactagattg aatgtttatc
      721 ataagtctga tatcatgttt atgaaatgaa aaaaggagcg gttatgatgg aaaaaatgcg
      781 tcagcaatta atggagtggg ggaaggaact agaaacattc cgtctgcctc actgggaaga
      841 actccctgac ttagaacttt atatggacca agtcagaacg ttagttgatc gttatctttc
      901 acctgtgata caaggagaaa agcatccatt gcttacctct tctatggtaa acaattacgt
      961 taaactaggt ttgataccag caccagttaa aaaaagatat aacaaagaac acgtagcatt
     1021 tttacttgca atcactacgt tgaaacaagt attgactatt cctgaaatca aagaagggat
     1081 attgttccaa ggaaaaacag taggaattcg tgaagcctat aatctatttt gcgatgaaca
     1141 agaagcagct gtgtggatgg tcagccaatt agcacaaggt aaatctcatc cgcaaaaatt
     1201 cgaccaggca acccctgtag aatatattgc tgtaagagcg gcaactttat cgtttgcaat
     1261 gaaacttctt gcggaaaaaa cgattgttct agaaaccgaa tatttaaaag aggagaaaac
     1321 aaatgaaaaa caataaaatc gcaattttag tagattcagg tacggatgtt ccagaagaat
     1381 tgataaaaaa atacgagatg tatatgatcc cattgaaaat tatttataaa gatcaggtat
     1441 atacagataa aattgacatt acccctgaag agatctatca acgtttgcca gaggaaatcc
     1501 ctggtacatc tttgccggat ggtgagtcga tcacagaaat ctttgagcaa atcaaaaaag
     1561 acggttatga aaaggtactt gcggtaacaa tttccagtgg attgagcggg acatataatg
     1621 tggttcgttt aattgcacaa cagcaagaag aattggaagc atatgtacta gatacgaaaa
     1681 atattggtat tggtgcaggt ttttctgcga tccaagcagc aaaatggctg gaagaaggag
     1741 tagaatggga tcaactgatc agtaatctga atgagttagt cgaaagaaca aaagttttct
     1801 ttaatgttgc gacattggaa tatcttcaaa aaggtggacg gatcggttta gttgcttcta
     1861 ttgtaggaac cgctttaaag cttaacccaa tcatctcatg taatgaagaa gggatttatt
     1921 acacagtagg aaaagctagg ggacgtaaaa aaagtttgga taaaacggtt gcttacgtca
     1981 aagaaaaaat cggtaatgca aaggtcttca acttagctgt tgcccatggg gatgcgaaag
     2041 aagaagcaat tgaaatggaa gcgagactta aaagagagtt cccacaagca aaccaaatct
     2101 actttggcca gatttctccg gcattagtag tccacactgg tccgggatta ttgggtgtcg
     2161 gtgtacaagt tttagaagga taaatcaaaa aaaggaaaca ctgaggtgaa aatcagtgtt
     2221 ttcctttttt attgaagtta gaacaaaaaa taataaagta ttttgcactt tcattttaat
     2281 tttctctttt gttttatagg caaagatgaa acaaaagcag aaatacggta taatgagaag
     2341 gtatgacaat aatatataag aggtggctct ttatgaaaaa agttttagtt gtcgatgatg
     2401 agaagccgat ttcggatatc gtcaaattca atttagccaa agaaggctat gacgtttata
     2461 ctgcatatga tggggaagaa gccttggaaa aagttgctga ggtagaacct gatttgattt
     2521 tacttgattt gatgctacct aaaatggacg gcttagaagt tgctcgtgag gtacgcaaaa
     2581 cttatgatat gccgatcatt atggtaacag ccaaagattc tgaaattgat aaagtcctag
     2641 gcttagagtt aggcgccgat gattacgtga ccaagccatt ttccaaccga gaattagttg
     2701 cacgtgtcaa agcaaatctt cgtcgcggtg ccacggctgc gaaggaacca gaagaggcag
     2761 cacctgcgga attgacgatt ggtgatttga cgatccatcc agaagcttac atggtgacga
     2821 aacgaggaga aacaatcgag ttaacacatc gtgaattcga actactcttt tacttggcga
     2881 agcaccttgg acaagtgatg acaagagaac atttattaca aacggtttgg ggttatgatt
     2941 attttggaga tgtacgaaca gtggatgtaa ctgtacgtcg tttgcgtgaa aagatcgaag
     3001 ataatccaag ccatccaaat tatttagtga cacgtcgtgg tgtcggttac tatttgcgta
     3061 acccagaaca ggagtaattg gatgaaaaga aaagtccgct tttttcgatc agtcaatttt
     3121 aagatcgcta ttacctttat tctgatttta ttgatctcga tcgaaattat tggtgcctac
     3181 tttatacgtg gtttagaacg ttccacgatc aatacattta ttaaagatat gaatcaaacg
     3241 gtggaatcat tagcaacaac aatcagcccg gaattgaatc gaaaagataa tgcagatgat
     3301 gaagaggtaa atgcgaatat caaaagattt attgaaaaca gtgcgactag cgatatcatc
     3361 gaaatacggg tcgttgacga aaaagggatc atccgaggaa caaccgatgt caatgaacaa
     3421 agcgctgtgg gaaagaaaaa tgattatgtt gatatcaatg atttcacgac taaacgttat
     3481 gttgctttag ataacgacga taaacgggtg aatatcaatg ttcaaccgat cctgtccccg
     3541 accggcgatg ccgttatcgg ggctctttac gtcaaaagta atatcgaaca aaaatattcg
     3601 gaaatcaata acacagcagt cattttcttt actgcctcac taattgcggc atttatttca
     3661 atgatcgtat ctgttttagt tgctcgttcg attacacagc cgatcggtga aatgagagaa
     3721 caagccattc gaatcgctag aggagactac agtcggaaag tcaaagtgca tggacaagat
     3781 gaacttggac agctggctga taccttcaat cagttagctg aacgtattga agaagctcaa
     3841 gatacaatgg aagctgaacg caatcgtttg gatagcgtgc tatcccatat gacagacggc
     3901 gtcattgcga cagatcgcag gggtaaagtc attacgatca atgatatggc tgtttccttg
     3961 ctagatatca aaaacgagga agcaatcgga caatcgattt tgacactttt agatatcgaa
     4021 gaagaatata cgttgcggaa attattagaa gatccaaacg aaatggtact tgaccgttca
     4081 accagtacat tggaaagtga tcagatgatc ttgcgagtag acttttctat gatccgtcgc
     4141 gaatcaggtt ttatcagcgg attagtagct gtcttgcatg atgtgacaga acaagaaaaa
     4201 acagaacggg aacgtcgtga attcgtctct aatgtttccc atgaacttcg tacaccgttg
     4261 acaagtatgc gtagttatat cgaagcattg agcgaaggtg cgtggaaaga cgaggaagta
     4321 gcaccaaatt ttttgaaggt aacgctagat gaaacagacc gaatgattcg aatgatcaat
     4381 gacctcttaa atctatctcg catggataca ggtaataccc agcttcaatt agaatatgtc
     4441 aatttcaacg agttagtgaa ttttgttctt gaccggtttg atatgatggt gggaaatcag
     4501 gaaaaaaatt ataagatccg tcgtgaattt acgcaaagag atctttgggt cgaagtagat
     4561 acagataaaa tcatccaagt agtcgataat atcatgaata acgcgatcaa gtattcacca
     4621 gatgggggaa cgattacttg tcgcttatta gaaacgcata ataacgtgat tttaagtatc
     4681 accgatcaag gattggggat tcctaaaaaa gatctgaacc gagtgttcga gcggttctat
     4741 cgagtagata aagcacgtgc aagagcacaa ggtgggacag gacttggatt agctatttct
     4801 agagaagtga ttaaggcgca tcgaggcgct atttgggcag agagcaagga aggaaaaggg
     4861 tcgacttttt atatctcgct gccatatgaa ccttatgagg aggaatggtg ggaatgagaa
     4921 tctctgaaaa agtcgttcgt ataggactca ttttgatggt tgcgttaagc atatacttct
     4981 cttatgcaat ctggctgagc ccagcaggaa aaagttcaat caacgtagat gagacaaaca
     5041 ctcaaatgat cgagtctcaa agcaatcgaa aaacttccga agtctttctt ccgcttcatg
     5101 ttacatggca ccattcggga atgatccaag aaaccaacag tgaaaatctc gtgtaccgat
     5161 tgcaattgat catcgataaa gcaagatttg gaaagctttc tgaagtagtt tcaggagaca
     5221 aatcacagtt tgaacaggtg aaaaatctga actctggttt tgagttggcc tataatgcgc
     5281 cttttttgct aagagaatac aaggaagcat ttgatttaga tttagatgtg tcgaacttgg
     5341 caaaagaaag cgaaaatatg tattttacta gaatacaatt cgatcaagga aataaaaaaa
     5401 tccgattttt gaattattcg aaccatctcg tatatgaagc aaatatttcg ttgaattgga
     5461 cagatatcaa taaagaatta acagcaaaag acacaaaatg gacagaaatg acaacagaac
     5521 catcgacgat cgacacgcaa tatgctacga aacattcgat caaactgaag aagtatagtt
     5581 atatcctgtc acagcaaccc tatacacttt ttagaaatgc ttttttccag aaaccagata
     5641 gcgtgaaaaa taatgatgaa agcaatgaat tgatttttta cggtgggaat gaaacaatgt
     5701 ctatccattc ggaaacgcaa gtagttgatt ttcgtggaga gctgccagag aaaaaagaaa
     5761 atgctagtgt attttctgaa agtttcccct atattagcaa attaggcagt tctttaggga
     5821 acctcagata ttttgaccgc agtgacaata cgatcgatta tcggatattc gtggaaggct
     5881 tcccggtttt tgggacagac agcagaggac agattggtgt ggaagttggc ggtgaaacga
     5941 atggttctgt gcaagtgaat atccaaacca gtttgaatac gattcaagtg ccgatacctt
     6001 cagacgaaga agtagaactt gcaagtacga atgaaatcat ccagcagtta aaagctaagg
     6061 gagcagatag caacaagatt ggttcagtga tcgttggcta tacatggcaa aatgtcaaag
     6121 aaacgaatcg tgtcgttgat ctcttacctg aatggtatgt gaaatataaa gataactggt
     6181 attcagctaa cgaccttctg gaaaaattac ctggattgga ggcgaattaa ttggatttca
     6241 aaaaaatcga atggatattt ttcgtcgctt ttttagggtt gaatgtgttt ttattcagca
     6301 gttatcatga cgcggtatac caagaaaaca atgtcattcg ctctaatcag actgaatcaa
     6361 tccaacaacg tctggctagc gacgatatca cttacacggg gaagatttcg tctgagaaca
     6421 agcaaggcta ttatcttagc gcggagcaga ccaatcttag cacttcatta gcgaattatc
     6481 gcaaggctgg taaaagcggc ttgctggcaa gtggtgtgtt tttagacggt gatgttttga
     6541 cgaaatcttt atccagttca gatgctgaaa agaacgtgat cgatccagat aaaattagta
     6601 gttccttgga agatttttta tctaacaaag acaatatttt attcggtaat gattatgtgt
     6661 acacgaaaaa cttttctcat ttagaagaag aaccactaga aatccaagca agccaagagt
     6721 atgaaggcat cccattcaat gatgatacag caaaaatcgt tctttcttta gaaaaaaacg
     6781 ataatgatta tagcatcacg aaatacatgc agacgcattt atctgatatc gagcagctgc
     6841 gcgaaaagac ggaactgtat acggaagagg aagcaatcaa tacattgtac atcaataaca
     6901 aaataccaag aggatcaaaa attttatggc gtcagctagc ttattcctgt attctgaaag
     6961 tccgggaaaa aaatgtgtat gttcctgttt ggcatgtagc gatcgaaaca agcgataaag
     7021 tggtccaaat tgagagcgtc aacgctttca gcaatacgat cgtgacaaac aatactgtac
     7081 caaaagtgga agatcaataa aaaagcgagt ataatgaaaa aagtttacaa agtaagaagg
     7141 gaagtaggag tcaaatggct gaagaaaaac cagcttttaa aatcagtatc ctagcaagtg
     7201 gaagttcggg taattcactt tatatcgaaa gtgaaaaaaa acggcttttg gtcgatgcag
     7261 gacttagcgg taaaaaaatc acttccttga tggctcaaat tgatcggaca ccggaccaat
     7321 tagatggaat acttgtgacg catgaacatc gagatcatat acatggcgtg ggtgtcttgg
     7381 ctcgtaagta tcatttggat gtttttgcca atcagcagac ttgggaagcg atggaaccac
     7441 tgattggaaa tgtaccagtg gaacaaaaac atctatttga gatggggaaa gtcagaacat
     7501 tcggcgatat cgatattgag agtttcggcg tctcccatga cgcagcggct ccgcaatttt
     7561 atcgtttcca taaagaaaat cggtcattcg tcatgttgac ggacaccgga tattgcagtg
     7621 attatatgcg aggtgtcatt gaaaatgccg atggttattt gatggaaagt aatcatgatc
     7681 ttgagatgtt acgcatggga ccttatccgt ggaatttaaa acagcgtatt ttaggagata
     7741 gaggacatct ttccaacgaa gacggtgcac ttgtcatgac tgatgtgatc ggtaatcgga
     7801 ccaaacgaat ctatcttggc catcttagca aggaaaacaa tatgaaagaa cttgctcatt
     7861 taacaatgga aaatgttttg aaagaaaaag attttggcgt tggacatgat tttgaaatct
     7921 atgatacaga tccagatgaa gcaactgagc tattttcgat ttaaacagtt caatcaataa
     7981 aaatataaaa agcagaagat aaaatcaatc agggttggtt ttatcttcta tttttatttt
     8041 tttagcatat taaaaaaata accgtattat cgactgacta cactgatgtg ctgcttttca
     8101 tgtttctcat ccagtatctc atctactaat gcaatcgcgt aatctgccat gctgatcgta
     8161 ctgtcgctag cagcgtttct ttgtaaataa tcatcactta acaaataagt acctgtacgt
     8221 ttgccatgtg gatcaaataa agaagctgga ctgatataag tccaatgtag atttttagat
     8281 tgtttcaatt ttaacaatcc ttcgtacata ttgaaagctg ttggatagaa gggtgcattt
     8341 ttaggtactt ggtcaatcat acgagtcgtc ttttctgtat ctaagaataa cgaagaggca
     8401 cccccaacga caattaagcg tgtatcggtt ccttcaagca tctcaatcaa atgggacaaa
     8461 gaagtttgat gcatttcttc ttttcctttg ggagcgttga aagcatcaac gacaacatca
     8521 aaagatgcaa gatcagtagt tgtcaactca aataaatcct tttgtataaa cggaacatct
     8581 gatggcaatt tttctggatg acgcacgatt gccgtcacgt ctaaatctct ggacaatgct
     8641 tcgtctaaaa taaatgaacc tgcatggcca gtagctccaa tgagtgcaat tttcataaaa
     8701 aacctccttt tttattcctg tccattgtaa gcagttgctg aatattcgtc aaagaatccg
     8761 atttttgaaa agctcagtgc gcattccttt catggttcgt gatcattggg tatagttaaa
     8821 ttaagaaaac tgattgataa aaatagaagt tgaaggagat aagaaatgaa agagtggtta
     8881 gatcgtgcct ttttaccaat gataacatct gttgtgaatc ttagaccctt aactgctttg
     8941 cgtaatggaa tgacttacac gatcccaata attattgttg gctcgctgtt tttcttattt
     9001 gctcattttc ctgtggatta catcgctcag tggatagaca aaacaggatt ttctatgtat
     9061 tttacacaag tataccaggc tacatttgat gtgatagcct tttggatcgt ttttgccatt
     9121 gcatacgctt atgcaaaaga agaagacatc gatcgcttac cagtcggcat aacagccttg
     9181 tctgcttatc tactgcttat tcagccacta aatactcaga tagatactgt ctggacaggt
     9241 gctgaaggga tactaggagc agttacaact gggctcatca tcggatgggg ctatgcacgt
     9301 ttagtaagac ttgcgaaagg gtggaagcaa agagagaaga tacctgaaaa tgtttggcag
     9361 tcttttactt cattgattcc aatcattgtt atagtcactt ttacgcttat cggttcagcg
     9421 gtttttcaac atttgttcag aatgactgtt gttcaaggag tatggacata tgtccagctt
     9481 cccattcaat cgttcataga cacgttgttt ggtgtgcttt tattaggttt ttttgttccg
     9541 tttttatggt tatttggtct tcatggatct gcgatggtca atgggttggt tagtcccgtc
     9601 ttgcaagcga attcattagc aaatgcagaa atcttagcct caggacaaga gttgacggtg
     9661 gcaaatggcg gacatatcgt tactcagcaa tttctcgatc agttcatgac ggttacgggt
     9721 gctggattga cgttaggggc tgtatttttt atgacggttt ttgcgaaatc gaagaaatac
     9781 agagaattag gaaatcttgc tcttttacca gcctttttca acatcaatga atcaattatt
     9841 ttttccacac cgatcgtgat gaacccgatg atggctgttc cttttatttt cgcaccggtc
     9901 gtttcaggat tgattaccta ttccgcttta tattttggat tcgttccttt atttacagca
     9961 gtccaagtcc cttggacaac accgccgatc ctctcgggtt tcttagtagg cggagtgcct
    10021 gcagcgattt tacaaggtat cgtgttgtca attagttttt tcatttattt cccatttttg
    10081 aaaaaaatag attctgcaaa ctgcaaaaag gaaagacaga caaaaaatga tggagcagca
    10141 gaaaagatga acgactaaaa aacaaaaata ctcctgtcgc tttagattgg gaaaatcacc
    10201 aatctaggat gacaggagta tttatagtca tgtttatact agcccttttt ggacagctaa
    10261 ttgaacaaga acaactaatg tgttcattcc tacgtgtgtg atcatcgatg tccagatccg
    10321 accagttgct ttgtactcta ggctgaagaa gaaacctagg aaaaagtaga ttagcagatg
    10381 accatcatta tgagcaaacg caaacagcaa agagctgatc actgctccga cccaaaaatt
    10441 tgaatatcga ctgatgatcc ccagtatact gcggcggaat acgaactctt ccatgatggg
    10501 tccagcaaca gttgtcgcca aaataaaagc gggtgcttcc atgatcatct ggatgatatt
    10561 ttgtgtattc tcggaagggg ttgctttgcc aaacaataca gattctaaca tgacagcgat
    10621 gctttgcaga atcaatgcga caaagattcc aaccaatcca taaataacaa tctttggagc
    10681 agaagctttt tttaccgctg attcgatcgc taagggttca cgttgcttaa aataaaaaat
    10741 agctaggaca acggcgccta gtatatacat gactgcagtt gctgtgatca attcagacgt
    10801 agattttaca agtcctatcg aagctaggat caagggtgag ataaaaatta gaccatagca
    10861 aaaaatgctg aggaagctat atttttttaa agacatgtaa ttcctccttt taagggataa
    10921 caagcatgca aggcaagctt atatcgattg atttcaatta tatcataatt tcaataagca
    10981 gctgtcagaa taaaatttct tttctttgat ttaaaagctt gcaaaaaaga gggagaatga
    11041 gtattattaa aaatgtaggt tagcactcga aggttaagag tgctaacaga ttaaaaaaag
    11101 agttatcgga gggatttatc gtgttaaaac cattaggtga tcgcgtcatt atcgaagtcg
    11161 caaaagaaga agaaaaaact gtcggaggaa tcgttttagc ttctgctgct aaagagaaac
    11221 cccaaactgg gacagttgtt gcagtcggtg aaggccgttt gttagaaaac ggtgaaaaag
    11281 ttcctgccgc tgtaaaagca ggcgaccaag ttatgttcga aaaatacgcc ggtacagaag
    11341 taaaatacga aggaaaagaa tatctgatcg tcgcaggaaa agatatcatg gcgattgttg
    11401 agtaaatttg gaagataaaa aaataatcaa aaataatcaa tgaggtgaat caatcatggc
    11461 aaaagaattg aaatttgcag aagacgcacg cgcagctatg ttacgcgggg tagataaatt
    11521 agcagataca gtgaaagtaa cattaggacc taaaggccga aatgtcgtac tagaaaaatc
    11581 ttacggctct ccattgatca caaatgatgg tgtgacgatc gcaaaagaaa tcgaattaga
    11641 agatcatttt gaaaacatgg gtgcaaaact tgtatcagaa gtagcatcaa aaacaaatga
    11701 tattgcagga gacggtacaa cgacagcaac tgttctgaca caagcaatcg ttcgcgaagg
    11761 tttaaaaaac gtaactgcag gtgctaatcc tctaggcatt cgtcgcggga tcgaattggc
    11821 aacaaaagca gcagtcgaag aattgcataa tatctcaact gttgttgatt caaaagaagc
    11881 cattgcacaa gtagcagctg tttcttcggg ttctgataaa gttggacatt tgattgctga
    11941 tgcaatggaa aaagttggaa acgacggtgt catcacaatc gaagaatcaa aagggatcga
    12001 aacagaacta gatgttgtag aaggtatgca gtttgatcgt ggttacttat cacaatatat
    12061 ggtaacagat aacgacaaaa tggaagctgt tctagaaaac ccatatatct tgatcacaga
    12121 caaaaaaatc tctaacatcc aagatattct gccattgtta gaacaaatct tgcaacaatc
    12181 acgtccattg ttgattattg ctgacgatgt agatggtgaa gcattgccaa ctcttgtatt
    12241 gaacaaaatc cgtggaacat tcaacgtagt agctgtaaaa gctcctggtt tcggcgatcg
    12301 tcgtaaagca atgcttgaag atatcgctat tttgacaggt ggtacagtaa ttactgacga
    12361 tctaggttta gaattgaaag atgcaacaat cgaaaactta ggaaatgctt ctaaagttgt
    12421 tgtagataaa gacaatacaa cgatcgtaga aggttctgga gaaaaagaag caatcgaagc
    12481 tcgtgtgcaa ttgatcaaaa accaaattgc tgaaacaact tctgattttg accgcgaaaa
    12541 attacaagaa cgcttagcta aattagcagg tggtgtagct gttgttaaag ttggtgcagc
    12601 tactgaaaca gaattgaaag aattaaaatt acgcattgaa gatgcattga atgctacacg
    12661 tgccgcagta gaagaaggaa tggtctcagg tggtggtaca gctctagtga acgttatcag
    12721 caaagtttct gctgtagaag cagaaggaga cgtggcaaca ggtatcaaga tcgttgttcg
    12781 tgctttagaa gaaccaattc gtcaaatcgc tgaaaacgct ggttatgaag gatcagtgat
    12841 tgttgataaa ttgaagaacg tggaactagg cactggtttc aatgctgcta ctggagaatg
    12901 ggtaaacatg gttgaagcag gtatcgtaga cccaactaaa gtaacacgtt ctgccttgca
    12961 aaatgccgct tccgtttctg ccttgttatt aacgacagaa gcagtcgttg cagataaacc
    13021 agaaccagca gcgccagcag ctcctgcaat ggatccatca atgatgggcg gtatgatgta
    13081 agaaaaagca acttgcttat ctacaactga tcattagtga aagacagata aaaagaggct
    13141 ggaacagaag cgtttaactc caagaaataa gaagaaattc acggaaattg cttttcaaat
    13201 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgctgt ttata
//
LOCUS       DAHUVV010000074        13212 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.077, whole genome shotgun sequence.
ACCESSION   DAHUVV010000074 DAHUVV010000000
VERSION     DAHUVV010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.077"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            147..1016
                     /locus_tag="NK767_002074"
     CDS             147..1016
                     /locus_tag="NK767_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HCH6695058.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="NK767_002075"
     CDS             1225..2250
                     /locus_tag="NK767_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCH6695059.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="NK767_002076"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="NK767_002076"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCH6695060.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="NK767_002077"
     CDS             3333..5036
                     /locus_tag="NK767_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCH6695061.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="NK767_002078"
     CDS             5038..7071
                     /locus_tag="NK767_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCH6695062.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="NK767_002079"
     CDS             7082..7522
                     /locus_tag="NK767_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCH6695063.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="NK767_002080"
     CDS             complement(7722..8849)
                     /locus_tag="NK767_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6695064.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="NK767_002081"
     CDS             complement(8852..9529)
                     /locus_tag="NK767_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6695065.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="NK767_002082"
     CDS             9793..11559
                     /locus_tag="NK767_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCH6695066.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="NK767_002083"
     CDS             11574..12011
                     /locus_tag="NK767_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCH6695067.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="NK767_002084"
     CDS             12024..13175
                     /locus_tag="NK767_002084"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCH6695068.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DAHUVV010000075        13013 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.078, whole genome shotgun sequence.
ACCESSION   DAHUVV010000075 DAHUVV010000000
VERSION     DAHUVV010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.078"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            50..1717
                     /gene="yfcC"
                     /locus_tag="NK767_002085"
     CDS             50..1717
                     /gene="yfcC"
                     /locus_tag="NK767_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCH6695069.1"
                     /translation="MIFGVVIFAWILTFVVPAGKFSTQDIEYKDANGETSTRTVLRQD
                     SFRYAYELDKSYVFDQLEELQDHPAEREKLDVPEKGLEKVIADGEKNLTQEKLDEISL
                     TDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGGFGFLNFVFEGLVSGDKYGSAVGIA
                     ALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLERLALPLLFFAFSFGGATFGMAEEV
                     IPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNATSWMSPFSVAVAQGIAGIPVLSGA
                     TFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLTYKSDDYFRSHIKKTSDENKKFMLG
                     HKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFFVMGLVAGIIAVLFKLDGMKVNDIA
                     SSFQSGAADLAGTAIVVGMAKGILLVLGGSDASVPSALNTILHGIGTALTGVPAVIGA
                     WAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADLVGVSRQVAVLAYQLGSGFMDAFTP
                     VSASLIGVLGVARIEWAKWARFQIKMQGFLFVLGTIFIMIAIAIGLQ"
     gene            1874..3058
                     /gene="iadA"
                     /locus_tag="NK767_002086"
     CDS             1874..3058
                     /gene="iadA"
                     /locus_tag="NK767_002086"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCH6695070.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3169..3372
                     /locus_tag="NK767_002087"
     CDS             3169..3372
                     /locus_tag="NK767_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCH6695071.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3473..4135)
                     /locus_tag="NK767_002088"
     CDS             complement(3473..4135)
                     /locus_tag="NK767_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6695072.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4148..5650)
                     /locus_tag="NK767_002089"
     CDS             complement(4148..5650)
                     /locus_tag="NK767_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6695073.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6198..7877
                     /locus_tag="NK767_002090"
     CDS             6198..7877
                     /locus_tag="NK767_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCH6695074.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8004..8483
                     /locus_tag="NK767_002091"
     CDS             8004..8483
                     /locus_tag="NK767_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HCH6695075.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8702..9382
                     /locus_tag="NK767_002092"
     CDS             8702..9382
                     /locus_tag="NK767_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCH6695076.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9390..10721
                     /locus_tag="NK767_002093"
     CDS             9390..10721
                     /locus_tag="NK767_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCH6695077.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10736..11698
                     /locus_tag="NK767_002094"
     CDS             10736..11698
                     /locus_tag="NK767_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HCH6695078.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11809..12147
                     /locus_tag="NK767_002095"
     CDS             11809..12147
                     /locus_tag="NK767_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HCH6695079.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12226..12933
                     /locus_tag="NK767_002096"
     CDS             12226..12933
                     /locus_tag="NK767_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCH6695080.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4121 a         2307 c         2867 g         3718 t
ORIGIN      
        1 aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg
       61 tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca
      121 agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga
      181 ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact
      241 gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt
      301 cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga
      361 tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa
      421 aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt
      481 tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct
      541 tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta
      601 tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc
      661 tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt
      721 gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta
      781 tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc
      841 tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt
      901 agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa
      961 accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac
     1021 atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt
     1081 tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc
     1141 ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga
     1201 tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg
     1261 tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc
     1321 atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt
     1381 tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt
     1441 tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt
     1501 agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga
     1561 cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg
     1621 ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat
     1681 tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa
     1741 agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga
     1801 gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa
     1861 agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg
     1921 aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg
     1981 cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg
     2041 tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg
     2101 aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg
     2161 tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct
     2221 ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac
     2281 agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga
     2341 aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt
     2401 agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt
     2461 aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc
     2521 gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt
     2581 ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg
     2641 ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga
     2701 tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga
     2761 tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat
     2821 caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag
     2881 taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc
     2941 agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga
     3001 aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc
     3061 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
     3121 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
     3181 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
     3241 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
     3301 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
     3361 gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat
     3421 atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt
     3481 cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt
     3541 caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa
     3601 gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga
     3661 ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg
     3721 aaagtttggt cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat
     3781 ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga
     3841 cgttgtctag agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat
     3901 catttttacg ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct
     3961 ttccttcttt tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac
     4021 cacttgctcc taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat
     4081 aaagcgcatc ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat
     4141 tcctccttta gtttaatagg atttttcttg ggtttaatct taatatccct atagaagcta
     4201 aaaagacaga aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag
     4261 gtttgacatt gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg
     4321 ttccttgttg accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac
     4381 cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc
     4441 ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac
     4501 aaatagccac ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata
     4561 gcaagacgcc taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta
     4621 tgataatacc ggctacagtc actagtaaaa caacattttt agaaaaactt gccacgttgt
     4681 ttaaaggttc caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg
     4741 tgtcgatcag cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag
     4801 tgtaaatagc agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag
     4861 tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc
     4921 cgcttgtttc gctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat
     4981 cttcagtact cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag
     5041 tagaatcaat gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg
     5101 tgatttttgc tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga
     5161 tttgaaagtc tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc
     5221 ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc
     5281 catcttttgc catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt
     5341 cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg
     5401 gacgttcttc agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt
     5461 ctcgattggc acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt
     5521 cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca
     5581 cggcaaacaa taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca
     5641 taaaattcat attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta
     5701 aagctagctt aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa
     5761 aagtcactat agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac
     5821 gaagaagaaa tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac
     5881 gtgcatagtt gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa
     5941 tggggaacag ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat
     6001 tttgttaatg aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc
     6061 tggtaatttt cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat
     6121 tggaatggct catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat
     6181 aagaagaggt gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa
     6241 atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc
     6301 taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt
     6361 tagaagaaaa cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga
     6421 tcggggcact tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga
     6481 cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt
     6541 tccatgtggt agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc
     6601 gtaccactca tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta
     6661 tcgtttatac gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt
     6721 atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa
     6781 atgcagaaaa tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata
     6841 cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag
     6901 tgcaacaagt attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag
     6961 atttcgaacg aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag
     7021 atctgcttgt tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag
     7081 aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc
     7141 gtaatgtcaa gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga
     7201 tggaaaccat cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt
     7261 tgatctctga gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga
     7321 tcaatttgat gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg
     7381 ctcatgcaga tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac
     7441 gcggagacgt gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag
     7501 aaaatgtaat gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc
     7561 gaaaaatctt gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa
     7621 agaaaatcat ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc
     7681 gtgatctgat gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa
     7741 gcaatgattt tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact
     7801 tatttgaaca aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac
     7861 gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg
     7921 ctcttttttt gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac
     7981 gatagaataa agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag
     8041 aacaaaagat cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag
     8101 aagaactaca aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag
     8161 gaaacaaaac tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa
     8221 aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa
     8281 tgcatacagg atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg
     8341 attattgttt tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg
     8401 gtgaaatcgg gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta
     8461 tttacgctga tttaattagt taagggacga gaagagaagc taagcagagc agagacctca
     8521 aacttgagat tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt
     8581 tgtacatact gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga
     8641 tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa
     8701 tatgaaaatt acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg
     8761 cgtgatgatt gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt
     8821 aaagacagat tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc
     8881 tattgcaaaa gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa
     8941 cagcagagga gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg
     9001 cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca
     9061 gtatatgggc gagcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc
     9121 aggggatact gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt
     9181 cgctttcttg ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc
     9241 gcaaacaatt cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga
     9301 acaagatccc gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt
     9361 ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat
     9421 atattgaaag tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga
     9481 atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt
     9541 caacaattca acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac
     9601 gtttgacatt tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag
     9661 gattagacaa ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac
     9721 gctatattac gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag
     9781 cattggaaga tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg
     9841 caaagctagc tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag
     9901 tcgcaaccat gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg
     9961 tcagtgatat ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt
    10021 ctgattatca gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc
    10081 atcatcgttt gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga
    10141 tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct
    10201 ctgttagcca tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact
    10261 tgacattgga aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc
    10321 gacaaccgca gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg
    10381 atactgaccg tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac
    10441 aaatggtcaa tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg
    10501 tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc
    10561 tacattattt gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg
    10621 aaatcggcag acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt
    10681 ccaaagcaat cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac
    10741 agtacagcaa gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca
    10801 tttgcgccct gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga
    10861 aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt
    10921 agcagaaatg caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga
    10981 tacagctaat tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa
    11041 aatcgaccat catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc
    11101 aagtagctgt agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat
    11161 gaacgttaag gctgcacggt tactttatgc aggaatcgtc ggagatacag ggcgcttttt
    11221 gtatccctcc acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt
    11281 tgatgcaagt gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc
    11341 cggttatctt tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc
    11401 gcaagaaatt cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact
    11461 tcctggagtg attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta
    11521 ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca
    11581 tggaggaggc catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga
    11641 aatctataaa gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata
    11701 cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc
    11761 tttttccgtt acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca
    11821 aattgcccag aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca
    11881 gaatgcggga acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg
    11941 gtattagatg caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac
    12001 ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt
    12061 ttagtagaag gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg
    12121 aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct
    12181 actactaatg ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga
    12241 atgtatggtt ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga
    12301 attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac
    12361 aatgtgactg gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc
    12421 ggtgttcaac aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg
    12481 agtttgtctc aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat
    12541 gaatgcggac atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca
    12601 cttgttccag cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta
    12661 ttgcttagct ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta
    12721 cttttccaat tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt
    12781 ttgagtgaag gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt
    12841 gcagcagcac tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg
    12901 atcttattgt tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt
    12961 tgatctgagc taataccgag agttagctga ttgaatacca tttatttgga ata
//
LOCUS       DAHUVV010000076        12738 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.079, whole genome shotgun sequence.
ACCESSION   DAHUVV010000076 DAHUVV010000000
VERSION     DAHUVV010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.079"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="NK767_002097"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="NK767_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCH6695081.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="NK767_002098"
     CDS             complement(322..891)
                     /locus_tag="NK767_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCH6695082.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="NK767_002099"
     CDS             1077..1979
                     /locus_tag="NK767_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCH6695083.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="NK767_002100"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="NK767_002100"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCH6695084.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="NK767_002101"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="NK767_002101"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCH6695085.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="NK767_002102"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="NK767_002102"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCH6695086.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="NK767_002103"
     CDS             8547..8732
                     /locus_tag="NK767_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCH6695087.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="NK767_002104"
     CDS             complement(8794..9990)
                     /locus_tag="NK767_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCH6695088.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="NK767_002105"
     CDS             complement(10160..11596)
                     /locus_tag="NK767_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCH6695089.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="NK767_002106"
     CDS             11889..12698
                     /locus_tag="NK767_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCH6695090.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4012 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattt
//
LOCUS       DAHUVV010000077        12619 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.080, whole genome shotgun sequence.
ACCESSION   DAHUVV010000077 DAHUVV010000000
VERSION     DAHUVV010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.080"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            119..2002
                     /locus_tag="NK767_002107"
     CDS             119..2002
                     /locus_tag="NK767_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCH6695091.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2077..2361)
                     /locus_tag="NK767_002108"
     CDS             complement(2077..2361)
                     /locus_tag="NK767_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCH6695092.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2351..3085)
                     /locus_tag="NK767_002109"
     CDS             complement(2351..3085)
                     /locus_tag="NK767_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCH6695093.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3214..3837
                     /locus_tag="NK767_002110"
     CDS             3214..3837
                     /locus_tag="NK767_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCH6695094.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            4029..5150
                     /locus_tag="NK767_002111"
     CDS             4029..5150
                     /locus_tag="NK767_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCH6695095.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5157..8285
                     /locus_tag="NK767_002112"
     CDS             5157..8285
                     /locus_tag="NK767_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCH6695096.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8304..11498
                     /locus_tag="NK767_002113"
     CDS             8304..11498
                     /locus_tag="NK767_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HCH6695097.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11644..12351)
                     /locus_tag="NK767_002114"
     CDS             complement(11644..12351)
                     /locus_tag="NK767_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCH6695098.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4300 a         2217 c         2658 g         3444 t
ORIGIN      
        1 ttaaatttat gggatgatag aaaaaaatag tagagaaagg acacgattct acttgctatt
       61 ttcacttaat acccgtatat tacgaatgaa aaaagtttat gaaaagttgg aatattgaat
      121 ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact gggtttgatg ggctccgttc
      181 attagctgtg gtgggtgtta tcttctatca ccttctgcca actagtatga aaggcggcta
      241 tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg atcacggatc tgctgcggca
      301 ggaatgggaa caaaatggaa aaatcaatat ttggcaattt tatgtcagaa gaatgaagag
      361 actctatccg gcacttgttt ttatgcttgt gacagcttcg gcttatatca ctttgtttca
      421 gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa
      481 ctggtggcaa atcaataacg gtttatctta tttcgatcga tttgctaatg agtcaccgtt
      541 tacgcatatc tggtctttag cagtagaagg tcaaaactat ttggtttggc cgatcttgtt
      601 tgtattgttg atggtatttg ttcgtaaaag aaaatggatc ttttatactg tgttaggtac
      661 atccttcctc tccgccatct tgatgatgat cttgtataca ccaggaggcg atccaacccg
      721 tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt
      781 tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa caagcgaaaa atattctgaa
      841 tatcgctgga ggaatctcgc ttttggcact gattctttgc ttcttctttt tagatgatca
      901 ttacaatttc atctattacg gaggaatgct cctagtgagt ctgttatgcg tagtgctagt
      961 tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg ctgacaaatc cagtatttac
     1021 ttggattggc aaacgaagct atggcattta tctgtatcag tttccagtta tgatttttta
     1081 tgaagcaaag gtgactgata ttgccgatca tgttttgttg catactttag tggaaatcac
     1141 attgattcta atcatcagtg aactttctta tcgatatgtg gagcgtccgc tagcacgttt
     1201 tgattatggt cgtacattcc aagtgatgaa aggatggttc agcaaaccga tcatcagtaa
     1261 gagcaaacca tgggttgttc caggcacatt acttgtagca attgcactcg ttggtttcgt
     1321 tacggcaccg aaaaacgctg taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga
     1381 aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa aacaccacta gttcggttga
     1441 ccaagcgatc cttgataagt acgagttgtc caaggcacaa gggaataagg cacaaaaatt
     1501 agaactaacg gcatttggtg attcagttat gttggatgct gcaacggatc tacaagaact
     1561 ttatccaaaa gtagtggtag atggagatgt cggacgacaa ttatatgcca gtgtaccgta
     1621 cattgaaaaa ttaaaggaac aagatttgtt gaaagacact gttctgattg gactaggaac
     1681 gaatggctca ttctcggaag ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa
     1741 agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat
     1801 gcttgaacag atgacgaaaa aatacgacaa tatgacgctg attgattggt acagctacag
     1861 taatgaccat agtgactggt tttatgatga tcaagtccat ccaaatcctg aagggatgac
     1921 acattacatt cacttagtca gcgaagcctt gctaggagga agttcaagta cgaacagcag
     1981 tgagacaaac gtgacagaat gatagctaca aaaaacttca ggcaaaaaca tttgtttttg
     2041 cctgaagttt ttttatattt accgtgtttt taacattcat atagattcag gaagcaaaac
     2101 ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc
     2161 ttctgctttt gttgcttcgc ttcttgaaga aaatacttct gcatggatca tcttaacagg
     2221 tcttcttttt tgaagacgag tatatttagc accagtacca ttgttatgtt cctctaatcg
     2281 tctagaaaga tcggttgtat atccaccata aaatgtctga tctttacaga ataatacgta
     2341 aaaataatgt tcatttgcca tagagcatct cacgaatctc tgcagtgtat tctccatcca
     2401 tattgtaagt gatcaatggt ggagcgactt tgaatccgtc taatttgcct tctttgattc
     2461 cttcaactaa caaaatattt gcctcttttc caggttttgg gtaaacaaat tggatccttt
     2521 taggtgcaat tcgatagtct tccatgacct gaatgatttc taagaaccga tcaggacgat
     2581 ggaccatagt aaagcgtcca tttgttttta gtagcttact tgaaatatcg atcacttctt
     2641 ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg tttattaggg ttcgttttat
     2701 ttgttggcag ccctttgaaa taaggtggat tacagacgac taagtcacaa gaattatgtc
     2761 gaacgacaga taagctgtct ttcagatcga tggtgtggac agtcatttgt tcatttaaat
     2821 ggttaagcgc gatactacgt cttgccatgt ctgctagacg aggttgaagt tcgatagcgt
     2881 cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa
     2941 ggtcaacaat cttccctttt ttaggtacac gggaaaagtg agcaagcagc acagcatcta
     3001 aagaaaaaga aaacactttg gaactttgga taatcttgat atcttctgcg tataattggt
     3061 cgattcgttc gtcttcgtga agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt
     3121 attatactat ttttattaaa aagaaccaag gtggcttgtc aaacctctat tttttatgca
     3181 tactatgacc ataagaaggg aaaggaagag actatgtttt atcgatttat gcgcggggta
     3241 gttcgagtac tattagcctt agttaatgga aatgcccgct atgagaataa agaagtttta
     3301 ccgcaagatg agaattacat actagttgct ccccatcgta cttggtggga cccattatat
     3361 ctggcggtag ctgcagcacc caaggaattc agctttatgg caaaagaaga actgttcaaa
     3421 aatccagttc tgcgatttat attaactcga tccaatgctt ttccagttaa aagagaaaat
     3481 ccaggaccaa gcgtcattaa aacacctgta aagattttga aatcgacaaa tttgagtttg
     3541 atcatgtttc ccagtgggac aagacatgct acagaactta aaggcggaat ggcattgatt
     3601 tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc aaggtccgtt gacacttggg
     3661 gatctgttca agcgtcagag agtgacagtg cgtttcggcg aaccaatcga cttatcagat
     3721 atcaagaaaa cggataaaga aggtatggcg gagatcgaac gacgaacaca agcagctttt
     3781 gacaaattgg acaaagaagt gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat
     3841 gataagttta tgcgtgggcg gtcttttgaa tctactgtcc tgttcagttc agactttttc
     3901 aatgaaaacg agggagagac gtcaagtctg ccctcttttt gcgttccaat atgtaaaata
     3961 gaagacaggt gtcatgtaat ggatcaaagt gttataataa ttagaaggac attttttgag
     4021 gtgattgaat gcgtttttta catacagccg attggcatat cggaaaaaaa ctacaaggtt
     4081 atgacttgat tgaagaccag cgtcatgtac tatcttctat actagatatc gcgaaagaag
     4141 aaaaagtaga ggcaatcgtg attgccggag acttatatga taggagtgtc ccagctgttg
     4201 atgccgtaga attatacaat gaattgatgg tcgattggaa tttaaaggaa aagatcccga
     4261 tttttgctat atcaggaaat cacgatagca gtacgagact ttcagcgggt acgccatggt
     4321 tcagttattc gaactatttt ttgcgaacac agctagcaga agcatttgaa ccagtcgagt
     4381 ttaaaaacgt acaatttttc tttcttcctt actttgaacc aattgcagcg agattatatt
     4441 ttgaagatga caatatccga acgatcaagc aagcggtagc caaggtgata gataaaatgc
     4501 aggagctatt tgattcagaa aaaaaacaag tattgattag ccattttttt gtaatgggaa
     4561 gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg gttggattcg atccctggtg
     4621 aactacttac accgtttgat tatgtagcac taggacattt gcataatcaa gctgctttga
     4681 gacaggataa tgctcgctat agcggttccc cattgaaatt ctctttatct gagatcaacc
     4741 aaaaaaaagg cgtatggatc gttgacctga caacaggcct ggagttgaat tttagggaga
     4801 tcgaacccct gcatgatatt gaagaaataa cagcgagttt caaagaattg ctggatcctg
     4861 ccttttatca gtccgtcaat cgggagaatt acatctatat ctatctcact gatagaacga
     4921 tcatcccgaa tatgatgaac cagcttcggc aaatatatcc tcgtattttg gggattgaac
     4981 gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa agtactagaa atgaaagatc
     5041 ctcaaaaatt agcagaagca ttttttagtg aaatgacgga agagcagcta actgcaaacc
     5101 aagaaaaatg gctcggagaa acgttgcaag aaatcatgga aatggaatga tcaagaatga
     5161 aaccgaaaaa attacaactt aaaaattttg gtccttttat caatgagacc gtagattttt
     5221 cacgtttgac agaagcaccg ctttttttga tcagcgggaa gacaggagct ggcaaaacga
     5281 cgatttttga tgggatcacc ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg
     5341 gaaaagagat gcgttctttg tttgctacac cagacgaaga aacaagcgtg actttttctt
     5401 ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac
     5461 gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac gttgaccatt tttgatggga
     5521 aaggaaaaga aataaaacaa atcacaaaac gtacagaagc agatcagttg atcagagaac
     5581 tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt actaccccaa ggagaattcc
     5641 gtaatttttt ggtttcatca agcagtgata aagagactgt tttacgtcat ttattcggta
     5701 ctcaattttt ccaacagttc aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa
     5761 aattggatca tttagagcaa gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag
     5821 aagaggaacc agcagcaggg tttgaagccg ttttagccca atggacaaac catcaggcaa
     5881 tactcgaaaa acaaatcacg gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa
     5941 agcaactaga aaattcctac tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa
     6001 cgatattaat aaacaagcaa aaggaattag ctgctcaaaa agacgaaata gaggaaaaga
     6061 aacgatggat tgggtattat gaatttacgc aacgattagt cgaaccaatc cagcgcctac
     6121 aagaaataaa agttgaaaaa atagaactgt cacaaaaaag tagtgagcaa acaaagaaac
     6181 tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc
     6241 gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa caaacagatg atggtgccaa
     6301 ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca attaacaatt tcattggata
     6361 aagaagaatt gaatgccgag aagctgtatc aaaatctcgc tatctatcag gaaagaactc
     6421 gaattcttca agaaaattta ctggatcagg aaaaactgca agaagatcgt cttgcttttg
     6481 gacagttaaa atatctagat gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca
     6541 ctgggtcgct cgcacagata agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa
     6601 atagtcagct attgaaagaa cagacagaac aagcagaaca gctgaaaagc caatgggcaa
     6661 agcagcaaat tgctcgtctt cagctttttt tgcgtccagg ggagccatgt cttgtttgtg
     6721 gatcaacgga acaccccata caacaattaa aacacgaaca gccaaataag gaagatatct
     6781 taaaagtaga acaacaactg gcagaagctg agtctgctgt agaaagaact cagcaaatca
     6841 ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca attatcagat ttagaacaaa
     6901 aaatcatggc aacgaatata caattggctg aacaagaaga acacttgcaa caggctttga
     6961 aaaaggcatt tcctgagata acagaacaaa aaacaaaaac aactcttgta gtaatcgaac
     7021 aatctttaga tagacgaaaa agcaagatgg ccaatgcaag aatagagctg gaagaaataa
     7081 gacaacaagc agaaaagctc gaacaacaat tagctgtcta caaacagcag cagacagaaa
     7141 aaaataatca gctccagcaa ctgcaaggag aaatcaaaag tttagaacaa caattgcaag
     7201 atacggatag taactgttta gaagaggaga tacgacgact tgaaaaacag ttagaagaag
     7261 actcgaatca actagctgtt gagcagaaag caggagtgca attgttgcaa gatcttcata
     7321 cgcttcggac acagcttgat cttcaacatg aacaaataga aaaaatagat gaaaaaatag
     7381 aaaaggaaca agaaagaata gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg
     7441 aagaaatctg tgcatttgca caacagcaag cacaatataa agaaaaccaa cgtacagtta
     7501 cttcttatca tgaagagcaa ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc
     7561 agctaactga gacattccca gatacagaag agatcagcca acagttagaa gaaataagac
     7621 gtatgatcga aataaagcaa gaaaagatat acggtttaca agaacaaaaa attagtaatc
     7681 aaaaaatcta cgatgaatgt ctttcgattt atcacacaag ccagacgaaa ttagatgagc
     7741 ttacgcaatt acagcagctt tcgcaaacat tcaacggaga aaatcctaag aagactagtt
     7801 tggaacggta tgtgcttcag gtttatcttc aagaagtgtt acaagtagca aatgatcatc
     7861 tccaacgttt aacgaaaaat cgttatcaat ttgagctagc ggataccatt ggcagttatc
     7921 gagggaaaac cggattagaa atcaatatct atgatgatca ggcaggtatg tctcgaagtg
     7981 cgcataccct gtctggcggt gagagtttta ttgcagcact ttctttagca ttagctcttg
     8041 ccgatgtgat ccagacacaa gctggtggtg tagcaatcga tgcattgttt attgatgaag
     8101 gatttgggtc attagatgaa gaagctttgg aaatggcaat ggaagcttta gaaacgatcg
     8161 aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag agaattgaaa gaacgagtat
     8221 tacaacaaat aagaattgat acagaaggta gcggacaaag caaaatacgc tatcaacttg
     8281 gataaaaagg gagattggta actatgaaat ggagtattcc agaaaaagtc attgcacgtg
     8341 gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg
     8401 tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt tgatttggat ggtacagcaa
     8461 aagagataga ttactgccaa tgtccctatt gggaggaaca taaatattgt aagcatacag
     8521 ttgctgtgga actttattta cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc
     8581 caatgctaga accggtaaaa gcaacaagtg agggagaaat cattgcaaaa ggtttttctc
     8641 gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt gattattgaa taccaagtag
     8701 aaacgattga gactaatcag tatcacccag agctttctat tttggcactt tacttgcgta
     8761 tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa aaacatctat gattttttac
     8821 aagcttacac agaaaaagaa acttacactg ttaataaaca gtaccagttt cgtctagata
     8881 aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca tcttgcagga agtgctcaga
     8941 cgcatcaatt attaggtacg aacggcttac aagtcaaagg aaaactagat aaaagatatc
     9001 tgcttctccc agtagaacaa gcccgcttcc tgttagagca gatgaatcag actacacgtt
     9061 tgcatctgca gataaatgaa caaaacgatc aaggaataca attttccgat caaaacaaac
     9121 ctttatcatt caaagtggaa aaatttggag aagattattt gctgcaagca ttgaatgatt
     9181 ttgatttttt ctttatgcat tatcagtggg gagttatcca agggacattt tattctctga
     9241 cgaaatatca acaaacggtt tatcaaactt ggaaacaatt gattcgccga ttggagaaac
     9301 cagaagtagt gttcaagaaa aaagaattac cgtctttgtt taaagagatt atccctctgc
     9361 tgtcagagat cggcgacgta gctattgccc ctgcagtaga agaggaagta gcagatatcc
     9421 ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt
     9481 tcgtctatgg tgaagtcatc tattcgactg acccgaagca tgaggtctcg acagaacagg
     9541 cttttcaggt gttacgtgac acagtagaag agaaacgagt acttgatcta ttcagaatgt
     9601 atcgctacca gaaaaatgaa actggttatg aacgtccatt gccaagcggg gaagaactat
     9661 atgccttttt ccgcacagaa ctatctgtct tccgtcagtt tggtgaagtc agacttggaa
     9721 gaaaattgcg agagttgtat ttagatgcgc ataaacatca accgaaaatc gaagtaaccg
     9781 agaccggttc atggctcgat attcgattcg atgtgacagg gatagaggag caagaaatcg
     9841 atcatgtcct gcaaagcttg ctgagaaatg atgcttttta tacattagaa aatggtcagg
     9901 ttctttcttt tgattcagaa gaatttcaac aaacaagcca agtgttgcaa caattacggg
     9961 aatcgatacg tacagaagaa ggaacgatcc atgtacctaa aaatcaagga ttgatcattc
    10021 aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc gttccaaacg atggtacaag
    10081 atctcattca tccagaaaga taccaggcac agcttcccaa aggattgaat gcaacgatga
    10141 gagattatca aaagcaaggt tttcgatggt tgaagatgtt aggtcattat cagtttggcg
    10201 gtattttagc cgatgaaatg ggacttggga aaacactaca gacaattgcc tttttacttt
    10261 cggaaaaaga agaacggaaa agcttctctg ctttgatcgt agcgcctgcg agcttaattt
    10321 acaactggca ggcagaggtg agaaaattcg ccccttcttt aagtatccaa gtaatcaatg
    10381 ggaataaaaa agaacgtgaa gaattactgg ctaaagacac agatatccga gtgacttctt
    10441 atgccagctt aagacaggat ctagctaatt atcagtcgca aaaaattgat tatttgattc
    10501 ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac tgcacaagca ttgagagaat
    10561 tagctgtacc tcagcggttt gcactgagtg ggacaccaat cgagaataat ttagaagaat
    10621 tgtggtcatt gtttgcaacg attatgccag gatttttccc gactaaaaca aaattcaggg
    10681 aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt tattttacgg cgagataaac
    10741 aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa tctatattct gcacttacag
    10801 aagaacaaaa aactgtttat ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa
    10861 tggattctga agcattcaag aaaaaccgga tcgggatact tgctggtctg acaagactgc
    10921 gacaaatttg ctgcgatcct cgtctgttta ttgaagatta ccaaggcggt tccggcaagc
    10981 tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa caaacgaaga gttttgctgt
    11041 tttctcaatt tacagggatg ctgactatct tgcaagaaga gttagccgag ctagggatct
    11101 cgacatttta tttaagaggc agtacgaaac cacaagatcg gttaagtatg gtagacgcat
    11161 ttaatgctgg cgaaaaagat gtttttctga tttccttaaa ggctggggga actggattga
    11221 atctcacggg agcagatacg gtcatcttgt atgacctttg gtggaatcct gccattgaag
    11281 aacaagctgc tggacgtgca catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta
    11341 tgatctctga aggaacgatt gaggaacgca tggattcact ccagcaagaa aaacgcgaac
    11401 tgttccaaaa agtgatccaa ggtaatgaag aacagttgac gaaactgact gaagaagaca
    11461 tccgcatgat tttaagtgta ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt
    11521 aatgggtaag aaagcctttc taaagaagcg attgtttatt tgatttaaaa aatagcaagg
    11581 aggctgggat tccattttat cccagcttcc ttgctatcat ttttttattt ctatttttaa
    11641 aggttataca aagattgtct tcattgtctc taacagctga tcaaaagaag aaatctcgat
    11701 atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca
    11761 gttagcactt tttgcaccta gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc
    11821 ttctggaagc atatgaaatt ctttctcagc cagctgaaaa atctcttttt ctggtttttg
    11881 gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga atccaattgt tcaattgaag
    11941 ttgcttcagt ttttttgtct gatggtcagt cggcccattt gtgataattc ctaatgggac
    12001 attcttttcc ttcaagaaat cgagggtttt tttgacctcc tcatgcaagc agatattatc
    12061 aagttcttcc tcgtaaattt tttgaaataa gaggccatct tcttccgtaa tatgagggta
    12121 gtctaaatct tttagtgatt ggctgatccg atgtgctcgc atatattcta gtgtccaatc
    12181 tcctgccatt acttttggga agttttcatc actatgatgg cggaaccgga tatatagtgg
    12241 atgcatatct gcatctgaaa caagcggaac gactcttttg accgcgtttc gaaatggctg
    12301 ctgctgatca tacatcgtat catcaacatc aaacacaact gatttaatca aaattttaca
    12361 cgcctttcgg ttttcattga ttttttcaca aatccttgac tattgtaaca gaaaaaattg
    12421 tctaaaaaag ctgataaata aaggtttttc ttactatatt tctatttcct tgacagaata
    12481 aggtttttat atttcagttt ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg
    12541 aacaaattaa tcattatggt agaagtcgat tgatttattt taatgcaatc tctgtggaat
    12601 tttcgaaaga tttattttt
//
LOCUS       DAHUVV010000078        12595 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.081, whole genome shotgun sequence.
ACCESSION   DAHUVV010000078 DAHUVV010000000
VERSION     DAHUVV010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.081"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            52..904
                     /locus_tag="NK767_002115"
                     /pseudo
     CDS             52..904
                     /locus_tag="NK767_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1001..2887
                     /locus_tag="NK767_002116"
     CDS             1001..2887
                     /locus_tag="NK767_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCH6695099.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFETPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(2928..3830)
                     /locus_tag="NK767_002117"
     CDS             complement(2928..3830)
                     /locus_tag="NK767_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCH6695100.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            4060..5463
                     /locus_tag="NK767_002118"
     CDS             4060..5463
                     /locus_tag="NK767_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCH6695101.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            5460..6896
                     /locus_tag="NK767_002119"
     CDS             5460..6896
                     /locus_tag="NK767_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCH6695102.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            7041..7967
                     /gene="trxB"
                     /locus_tag="NK767_002120"
     CDS             7041..7967
                     /gene="trxB"
                     /locus_tag="NK767_002120"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCH6695103.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            8181..9908
                     /locus_tag="NK767_002121"
     CDS             8181..9908
                     /locus_tag="NK767_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCH6695104.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            9924..10274
                     /locus_tag="NK767_002122"
     CDS             9924..10274
                     /locus_tag="NK767_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCH6695105.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(10369..11229)
                     /locus_tag="NK767_002123"
     CDS             complement(10369..11229)
                     /locus_tag="NK767_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCH6695106.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            11321..11749
                     /locus_tag="NK767_002124"
     CDS             11321..11749
                     /locus_tag="NK767_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCH6695107.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            11911..12087
                     /locus_tag="NK767_002125"
     CDS             11911..12087
                     /locus_tag="NK767_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCH6695108.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            12114..12560
                     /locus_tag="NK767_002126"
     CDS             12114..12560
                     /locus_tag="NK767_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HCH6695109.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         4119 a         2171 c         2775 g         3530 t
ORIGIN      
        1 ttttcttttt gttttggata cagccctata tatcatggaa aggagcaaaa catgctgatt
       61 gagaaagtac tgaataacaa cgtcgtaata accagggatg aaagaaatca ggaaatgatc
      121 gtgatgggaa aaggtttggc attcaaaaag aaagtgggtg acccgatttt tgactcatgc
      181 atcgacaaaa aatttcagtt gacaaatgac gggctttcac aacagttcca agaattggca
      241 caagcgattc ctcttgagta cttagaaatt tcttgtgaaa tcattgaata tacaaaagag
      301 gctctacaag tccagttgaa tgattctgtg tatattgcat tgacggatca tttgtatacg
      361 gctatcgaac gtcacaaaat gggaatattt gtaccaaatg tattgctgta tgatatcaaa
      421 caactatttc ctaaagagta tgccatcggg aaaaaaaaca gtgaagaaaa tctcggataa
      481 ataccacatc aagttatcag aagatgaagc cggatttatc gcattgcatt tagtcaatgc
      541 acaaggcatt caaagccaac aagacaatac ctacctgttc actataacaa tacaggatat
      601 tctaaatatc gtccgctatt actataagat tacatttgat gaagattcag tccatttttt
      661 tcgattcgtc acacacttac gtttttttct gacacgcgtc ctagatggag aaaaatcaga
      721 agtgggagaa gtttctgagg aattgtttca ggttattcaa aaaaaatata aggatacgtt
      781 tggttgcgtt gagaaaatag cagacttttt agccgagacg ttcaactacc agatgtccac
      841 ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta gtgcatgaga atcgttgtaa
      901 ataaataaaa aaaggaggat aaattaacaa gaacgtatga aataagggta gtgacattca
      961 gttggctaaa ccttccacta ataaattgga gggaatagaa atgggaaaat accaagaatt
     1021 agcgagaaaa atcgtagaaa atgtcggcgg gaaggaaaat atcaatggtt tgacacattg
     1081 tatcacaagg cttcgtttca aattaaaaaa cgaagaaaaa gcaaatgatg agatcttgaa
     1141 aaatatggac ggtattgtaa cggtaatgcg agcgggaggg cagtatcaag ttgtcatcgg
     1201 gaatcatgtg ccagtcgtgt ttgaagaggt tataaaagca ggaaacctta cttttgatga
     1261 ggctgtttca acagaaaaaa tgagaccgtt tgatatgctt attgatggaa tatcgggctg
     1321 tttccaacca ttccttgcta tcctagctgc tgacgggatg attcgtggac tgacagcatt
     1381 ccttgtcttc ttaggcgcgt ttgaccgcgg ttctggcaca tttgttatgt ttgataatat
     1441 cggtgattct gtattccaat ttatgcctgt tattatcggt ttaacagctg cccgtaaatt
     1501 taaagtaaat gaattcgtag ggatgttgct tggcgcagca ttaatgaatc ctagcttgtc
     1561 gctggaagct ttgagtggtg cagcagaagc accactaacg accatatttt ctggcactat
     1621 ttttgaaaca cctatttatc aaacagtatt cggcattccc tggattgcgc gtaactacgc
     1681 ttcttctgtc attcccatta tctttattgt gttgttagct agccaaattc agaaaccaat
     1741 taaaaagtta gtacctgaga tgatcgcgaa tttcttcgtt ccattcttca ccgttcttat
     1801 tacaatgcct ttaggttttt tactcgttgg accagtattc acattcgcaa ctgatatttt
     1861 aatggctgga tttgaaacac ttttagcact ttcgccagtt atttatggag caatcgtagg
     1921 cttcttctgg caaattctgg tgatgtttgg tctccattgg gcaatcgtac caatgggatt
     1981 gatgcaattt tctgtgaatg gttggcaaaa tattatgaca cctgttgcag tagtgtcttt
     2041 tggacaaact gcagcactta ccgcactcta ctttaagctt agaaatccaa aagacaaagc
     2101 aattgcaata ccagccattg tttctggtat tgtggggatt acagagccag ctatttatgg
     2161 gtttacattg ccacgtaaaa aaatatttat ttttacctgt gtcggtggtg cgttaggggg
     2221 cgcgtatagt gggttaatga atttaacttc ttggaatcaa ggtggactag gcatctttac
     2281 aattcccaac tatattagac cagatggtga tttaacagat gtgattaatg tattgattgg
     2341 gatcgccatt gcgatggtgg tatcctttgc gctgactttt ttcttttgga aggaggaagc
     2401 agatgaaaca gatgatagac agaaaagatc aggtaaagaa attgtaaaaa caccgataca
     2461 aggacaaatc gctccactga atgcagcaaa ggatgcagct tttgcacaag gaacactagg
     2521 tcgaggaatc ttgatctacc ctgaaaaagg agaagtacgt gccccttttg acggaacagt
     2581 catgtccctg tttccgacga aacatgcaat cgggatggtt tctgaagcag gtctggaatt
     2641 gctgattcat gtcggattgg atactgttca gttggaaggg aaatattttg aaagcttggt
     2701 gcagcaagga gaaagagtga aacaaggcga tctgttgctt cgcttcgaca aaaagaaaat
     2761 cgaagaggaa ggatacagtt tagaaacacc tgtgattgtc atcaattaca tggattattt
     2821 ggatattttg gaaaatcaag gggaaacggt cgggccagac gatgcactta tcaccgcact
     2881 gacctgaacg gttaggtgaa agaatggact aggcttaact attcttttta gtccacttct
     2941 ttattttcaa aattgaaagg ctctttcatg atggctgagc tgatagggcg tttatcaaat
     3001 ttttgtccta cagaaattag atggactaaa ttctgttgat aattttctgc aaaaacacaa
     3061 ctatataaca cgtctagaag ataacaaatc gatgtattga tcgtaaaatt accaattttc
     3121 gaatacagtc gttcgcgtgt cgtaagtcgc aaagcggctg tactcattga agatagcgtt
     3181 gaatcaccga tactggtcaa agctaaaatt ggcacatgat tttgtttcaa tatttctgcg
     3241 gtacgaagga tcacattgtt ttcacccgta taagaaatca gaatcccaca catatcactt
     3301 gtgcaattgt acgcttcata gaacgattca ccaaaagtgg tgacaatcgt gacatccatt
     3361 ttgatgcgct tcattttcag catgaaatct tgagaaatca aagtattcga gttactggcg
     3421 aaaatcacga ttttttttgc ttgaagcatt agttgtttgg ctttttgcag ttggtcatga
     3481 tgaatgagag aaagtgtatc ttgaatcgtc atctgttcta aagaagccat tttatttgcg
     3541 atcgtcataa taccgtctgt ggtggaaaaa ggaagattcg catctacgtc ttgaaaattc
     3601 ccttcaagat aggctgtttc agctaaaaaa gcattacgaa aactgaccca gccatcaaaa
     3661 cccatttttt ttgctacacg gatgagtgtg gacgggtgga cataacatcg ctcagcaatc
     3721 tcttggatgg tcatttcgcc aagtgctgga gaatcgctta agagaaactg aatcacttca
     3781 ttttctgaag gagaaaagga gatttctttg attttttcac gtaataacat gtagatacct
     3841 tcttcttgtt tttttacgta aattataact tttttgaaaa tgatttgtaa acggatttat
     3901 gttatatagc ttaataaaga cattttttga caagatattt acaaaaatcc agaagaaaac
     3961 actaactttt gtaaatgtta ctggaaagaa acgaatttct attgtttgtt ttgtttacaa
     4021 aaaagataat aaaagtgtaa aaagtaaagg agtgagaaca tggcaaaagg aatcaaaatc
     4081 gctacgattg gtggaggcag cagttacacc cccgagttga tggaaggatt tatcaaaaga
     4141 tatgaggaat taccgatacg agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag
     4201 cttgagatcg tcagtcagat gtctcagcgt atgtgggatg cttctccgta tgaagtcaaa
     4261 atctatgcaa ctctggatcg ggaagaagca ttgaaagatg cggattttgt tacgactcag
     4321 tttcgtgtcg gtcaattaga tgcacgaatc aaagacgagc ggatccctag ttattatggg
     4381 atgattgggc aagaaacaaa tggtgctggc gggatattca aagcttttcg taccgttcca
     4441 gtggttttgg caatcgttga agacatgaag cgtttatgtc caaatgcttg gctgattaat
     4501 ttcgcgaacc caagcgggat ggtgacagaa gcagttatca gatatggcaa atgggataaa
     4561 gtgatcggat tatgcaatgt gccagtaatg gcgatgatga cagaaccaac aatgcttggc
     4621 aagaaaaaag aagagctgat ctacaagttt gccggtctaa atcacttcca ttggcacaaa
     4681 gtagcagata ataaaggaaa tgaccgcacg gcagaattga ttgatcgttt atacgatgaa
     4741 gacaatggat tgccgaaaaa tatctttgac atcccgtttt tcaaagaaca attaaaacaa
     4801 atgaacatga tcccttgtgg ctaccaccgc tattactacc gtgaagaaga aatgctggaa
     4861 catgctttag aagaatatca aacgattggt acaagagcgc agcaagtgaa agaaacagaa
     4921 gcagagctat ttgaattata caaagatcca gaattagacc ataaacctga acaattgcaa
     4981 aaacgaggtg gcgcttatta ttcagacgct gcctgtgaaa cgattgcttc aatctatgga
     5041 aataaagaaa cacagatcgt ggtatctaca aagaatgatg gagctgttcc tgacctgccg
     5101 gcagattgtg tagtagaagt cactgcctat gttggcgcac aaggagcgag aaatgtagcg
     5161 tttggttctc ttcctgctgc tgaaagaggc tggttgcaag taatgaagaa tatggaattg
     5221 ctgacgatcg aagcagctgt gactggtgat tatgggacag ctttacaggc gtttacgatc
     5281 aatccgctta tacgatcagg aaagacagca aaacggatca tggatgagtt gtttattgca
     5341 caccagcagt atttaccgca attcaaggaa acaatcagcc ggttaaaagc agaaggaatc
     5401 gaagtccaag atgaactggc aagagaatta tcaagcaata cacatgtaat ggaggaagta
     5461 tgatgggatt cagaaaagat tttttatggg gcggagctac agcagccaat caatgcgagg
     5521 gcggttatga tgaaggcgga cgtgggttag cgaacgtaga cgttgtgcca atcggagaag
     5581 accgttttcc tgtcattact ggaaaaaaga aaatgtttga atttgatgaa aatcattttt
     5641 atccagcaaa aattgcgatc gatatgtatc atcattataa ggaagatatt gctttatttg
     5701 cagaaatggg gttcaagact tatcggatgt ccattgcttg gagtcgtatt tttccaatgg
     5761 gcgatgaaaa agagccaaat gaatctgggt tgaaatttta tgaggatgtc tttaaagaat
     5821 gtcaaaagta cgggatcgag ccgcttgtga cgattacaca ttttgattgt ccgatgcatt
     5881 tgatcaaaga atatggtgcg tggcgttcca gagaactcgt tggtttttat gaaaatctgt
     5941 gccgagtcat cttcaatcgc tataaaggac ttgtcaaata ttggttgaca ttcaatgaga
     6001 tcaatatgat ccttcatgca ccattcatgg gcgcagggct gtattttgaa gaaggagaaa
     6061 atgaagaaca aatcaaatat caagcggcac atcatgagtt ggttgcaagt gccatcgcaa
     6121 cgaagattgc tcatgaagta gatccggaga acaaagtagg ctgtatgctg gcagcaggag
     6181 catattatcc atacacttgc aatcctgaag atgtttgggc ttctagaaaa tcagatcgag
     6241 aaaattattt cttcattgat gtccagtcac gaggagaata tcccgcttat gcattgaaag
     6301 aaatggaacg aaatggcatt cagatcgaaa tggaacctgg ggatgccgca ttgctgaaag
     6361 aacatactgt tgattttatc tccttttctt attattcttc tcgagtttcg actactgaca
     6421 acgaactctt ggaacagact gcaggaaata tctttgcttc tgtcaaaaat ccatatctgg
     6481 aagcaagtga atggggttgg caaattgatc cattaggctt gagaataacg atgaatgata
     6541 tttatgatcg ttatcaaaaa ccactattta ttgtagaaaa cggcttaggt gcagtcgata
     6601 cgcccgatga gaatggttat gtggaagatg attatcgaat cgactatctg gcagcacata
     6661 tccaagcaat gaaagatgca gtagaattag atggagtcga tctattaggc tatacgactt
     6721 ggggatgtat tgatttagta tcagctggta caggtgagat gaagaaaagg tacggattca
     6781 tttatgtcga tcgtgacaac gagggaaatg gcacattagc ccgttcgaag aagaaatcat
     6841 tcaactggta taaaaaagta attgattcaa atggagaaaa tctttcgaat gattgatcaa
     6901 tgatttataa aagtctattg tttttcagcg gatttttcga gtataataaa gctattcgag
     6961 aaacgcaagc agaacaatag actttttttc gcattggctg taaagtatgc ttacttgatt
     7021 aagtaaagga gaatctacat atgtatgatg taatcgttat tggcgcaggt cctgctggga
     7081 tgactgctgc tttatatgct tcacgttcaa acttatccgt attgatgatc gaacaaggcg
     7141 cccctggcgg ccaaatgaac aatacggcag aaatcgaaaa ctatcctggt ttcgattcaa
     7201 tcatgggtcc tgatctagct tataagatgt atgaaggtgt ttcaagattt gggacagaaa
     7261 atgcttatgg aatcgtgcaa gatatcaagg atcacggcag ccacaaagaa gttatttgtg
     7321 aagataaaac ttacgaagga aaaacagtca ttatcgcaac aggctgtatc caccgtaaat
     7381 taggcgtacc tggcgaggaa gaatttgctg gacgtggcgt ttcttactgt gcagtttgtg
     7441 atggtgcgtt tttccgtaac aaacgtctag tagtgatcgg aggaggagac tcagcagtag
     7501 aagaagccat ctatttgaca caatttgctt ctgaagtagt catcgtccac cgtagagatg
     7561 aattacgtgc acaaaagatc atccaagacc gtgcatttgc gaatgaaaaa atttccttta
     7621 tgtgggatac agtcgttgac gaaatcgttg ggaatgaaat ggtagtgaca ggtgtgaaag
     7681 cacgtaatgt caaaaccggc aatgagtttg atttggcagc agacggagta ttcatctatg
     7741 ttggactgga tccattaact gcaccattta gagaagcagg gttgacgaac gaagctggct
     7801 ggattccaac agacaacgaa atgaagacga atataccagg cgtttttgcc atcggagatg
     7861 ttcgtgaaaa agatttgcga caaatcacta ctgctgttgg tgatggtggg atcgctggac
     7921 aacaagtgta taaatacttg gaatctttag aagaaacaac tgcataagtt taaaaagagt
     7981 ggaagaacgg tttcaattgt tcttttactc ttttttttcg cgtgaaatct actgaatccg
     8041 atttatgcga atacttgcaa ccgtgcttga taaaagaatc ctttcttgat ttaagcgttt
     8101 tttggtcctc atacttttat atctaataag tgaatgctat acttattaag aaccatattt
     8161 cagaaagagg tgttattttc atgtcttggg aacaagtgta tgaacaatgg gtacatgaag
     8221 aaaatttaga agaaaactta aaaaaacaat taaatgatct gggtgaagat cctgaaaaac
     8281 ttgaagatgc tttttatgca ccgttggaat tcggtactgc aggcatgcgg ggaatcttag
     8341 ggcctgggat caatcgaatg aacatctata cggtaagaca agcaacagaa ggacttgcac
     8401 gtttcatgga tacacaaggc gaagaaacaa aacgccgagg tgtcgctatc gcttatgact
     8461 cacgccatca atcaccagaa tttgccatgg aagcagccaa aacattagca catcacaata
     8521 tcccatctta tgtattcgaa agtctacgtc caacaccaga gctttctttt gcagtccgtc
     8581 atttgaagac gtttgcggga atcatggtaa ctgcatccca caatccagca gcttacaacg
     8641 gatataaagt ctacggcgaa gatgggggac aaatgcctcc tgcagacgca gatgcattga
     8701 cgaaatatgt ccgtgaagtc agcaatccgt taaaagttga agtattatct gacgaagaag
     8761 ctgaacatag cggattgatc acgatcatcg gagaagaagt ggatgccgct tatctagaag
     8821 aaatcaaagc agtaacgatt gatcatgaat tggtggaaac aatggggaaa gatttgaaac
     8881 ttgtgtacac accacttcac ggaacaggaa aaatgttggg cgaacgtgcg ttgaaacaag
     8941 ctggattcga acaattcgta ctagttccag aacaagctgt cgcagaccca gatttcacta
     9001 cagtcaaatc accaaatcca gaagaacact ctgcttttga gtatgcgata cgacttggcg
     9061 agaaagaagg cgctgatttg ctgattgcaa cagatcctga tgcagaccgt ttaggtgcag
     9121 ccgttcgttt gccaaatggc tcctatgaag tattgaccgg aaaccaaatc ggagcactgt
     9181 ttgctcaata tattttggaa gcacaccaat cgcgaggcac tttacctgaa aacgcagcgg
     9241 tattgaaatc gatcgtttct agcgaattac ctacagcaat tgcggaacac tatggggcta
     9301 ccatgttcaa tgtattgaca ggattcaaat tcattgctga aaaaattcaa caatttgaag
     9361 aagatcattc ccatactttt atgttcggat tcgaagaaag ttacggttat ttagttaaac
     9421 ctttcgttcg cgacaaagac gcgatccaag cattgctctt attagcagaa gtagcagctt
     9481 actataaaaa acaaggcaaa acgttgtatg acggcttgca aacgattttt gaaacctatg
     9541 gctactacgc agaaaaaacg atttctgtga caatgagcgg acaagaaggt tctgcgaaaa
     9601 tctctgcttt gatggaaaaa ttccgtgaag aagcaccaac agaatttgcc ggagcaaaag
     9661 tcatccaaac agaagacttc aaagagttga cgagaacaga ccgtgaagga aacgtagaaa
     9721 aactaacgac accgccatct gatgtattga agtatacgct ggaagaaaat ggctggatcg
     9781 ctatccgtcc ttcaggaact gagccgaaaa tcaaatttta catcggtgta aaaggaaact
     9841 cttcagaaga cgcagctgaa aaagtagccg cttatgaagc gattatccaa aagatgacag
     9901 aagaataaaa aaacttgaga ctgatggaaa aagtggtaaa ctttagaagt cagtcttttt
     9961 tcaagttgga ggtgaatgga ttgccagaca cgcaaaaaac aaaagtagag aaagtcagcc
    10021 aactattcaa aatgttgagt gaccccactc gtctcaaaat tctgctgtat ctgaaagacg
    10081 gggaacaaaa tgtgacagca atcacccaag cagtggaaat ggaacaatct gctgtatcgc
    10141 atcaactgcg tttattaaga gaaaaccatg tcgtcaagtc ccatcgtgag ggaaaagcga
    10201 ttttgtatag tcttgatgat tctcacgtac tggatatttt gaaccagaca ctcaaacatg
    10261 tggaacatca ataacgatag gtgcaaatag agacaaactg gactgtgtca acgaatcttc
    10321 ttcgctgaca cagtccagtt ttttttaaga aaaagacaag aaaaactgtt agtctgtttc
    10381 ttcggtggtt tcagttccat aataactatg atcttctagg tctaaagcat tcaaaataag
    10441 cgaagccgtt tcttcaatag ataaagatgc cacattgatc actacacatc ctagtttttc
    10501 ataaagatca ttcgcaaaat cgagttcagc tctaattttt tcgatatcgg aataagcagt
    10561 atcagggttt aatccgtaag agcgcatccg ttctttacgg atattattta aaatatctgg
    10621 gttattcgtt agtcccacga tttttttagg atcgatttcc cacagctgtt tgggaatatg
    10681 tgcttgggga atcaaaggta aattagcaac ttttaaattt ttatttgcta aaaacaagct
    10741 caaaggcgtt ttggaagttc gggatacgcc tagcaaaacg acatctgctt ctaaaaaacc
    10801 acgaggatct ttcccatcat cgtattttac agcaaattcc attgctttga tccgtttgaa
    10861 atagttctca ttcaataaat ggagggcacc aggttgccga attggcttga taccagttaa
    10921 acgttcaatt tctgatatcg gtggcgtcat cacatcgaaa ctaaataatt gatgttcttc
    10981 acaaaaagat cgtgttatct cgatcagttc ttgattgatt aatgtatgca ggacaagtgc
    11041 tttttcttct aatgcatctt gcaatgcctg ccttaatgtt ttttcatctg tcacgaacgt
    11101 tctgcggaat aggttgaact caacggtagg atactgagcc atagtagctt gcgctagttt
    11161 tgtcgcagtt tctccagcag agtcggagat gacaaaaaaa gtaacgattt ctgtagaatc
    11221 ggaagacatg gataaaacct cacttgcatt ttttttatgt ttattatacc atactctaga
    11281 atgcgtactt cgtaatcatt ttgatttgga ggaataagtc atgaaaaaac agacattgat
    11341 cgaacaatca ttagaaacat tgaaaaataa cggttttaaa tatactaaaa aaagagaagc
    11401 attgttaact tatttagtga agcgtaaccg ctacgtatct gctaaagaag tttttgacta
    11461 tatgaatcag gaattcaaag gggtaagtta tgatacgatt tatcggaatc tccatgattt
    11521 tgcagagatc gatttactgg aagaaaccga tttaaatgga gaaatgaagt ttcgttttca
    11581 ttgctgtcag ggggaaatcg gccaccacca tcattttatc tgtacagtgt gcggaacaac
    11641 gaaagaaatc catatgtgtc cgatgaatta tttcgaagaa cagctaggtg gatgcacgat
    11701 cgaagggcat agattcgaga ttttcggaag atgtgaaaat tgtcaataag gatttaaata
    11761 aaataacatt tgataattta aaaaaaaagc aagatttcct tgacggtcaa aacagcatta
    11821 gatataatat ttacaggaca gtcttgtttt ttgagatata tttcaataac aattacggtt
    11881 actttttaag ctcggaggga gggaattaac atgtcaaaaa cagtcgttcg caaaaacgaa
    11941 tcacttgacg atgctcttcg tcgcttcaaa cgttccgtgt caaaagcggg tactttacaa
    12001 gaatctcgta aacgtgaatt ctacgaaaaa ccaagtgtaa gacgcaagaa aaaatctgaa
    12061 gcagctagaa aacgcaaaaa attctagttc tgtgatgaat tggagttggt agtgtgtcac
    12121 tactaagtag attaaacgat gacatgaaag cagcgatgaa agcgaaagat aaggaatctt
    12181 tacaagtcat ccgaatgatc aagtcatcta tacaaaacga gcagattaaa gaaggtcacg
    12241 acttgacaga agaagaagaa ctaacggtgt tgtctcgcga aatgaaacag cgacgtgatt
    12301 ctttgcatga gtttgaagaa gcaggacgtg acgatttagc tgaaaaagta aaaagtgaaa
    12361 ttgtaatagt cgaaaaatat atgccggaac aactttccga cgaagaaatt cgacaacttg
    12421 ttcaagaagc aatcactcaa actggtgctt catcaatgaa agaatttggc aaagttatgg
    12481 gtgcaattat gcctaaagtc aaaggaaaag cagatggaaa ccaagtgaat gcgattgtga
    12541 aagaactatt acaatcttaa ttttatcatt taaactgcgg acagtgattg tccgc
//
LOCUS       DAHUVV010000079        12587 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.082, whole genome shotgun sequence.
ACCESSION   DAHUVV010000079 DAHUVV010000000
VERSION     DAHUVV010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.082"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            366..1946
                     /locus_tag="NK767_002127"
     CDS             366..1946
                     /locus_tag="NK767_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCH6695110.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            2144..2368
                     /locus_tag="NK767_002128"
     CDS             2144..2368
                     /locus_tag="NK767_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HCH6695111.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(2573..2893)
                     /locus_tag="NK767_002129"
     CDS             complement(2573..2893)
                     /locus_tag="NK767_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCH6695112.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(3041..5275)
                     /locus_tag="NK767_002130"
     CDS             complement(3041..5275)
                     /locus_tag="NK767_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCH6695113.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            5754..6020
                     /locus_tag="NK767_002131"
     CDS             5754..6020
                     /locus_tag="NK767_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCH6695114.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            6020..7747
                     /gene="ptsP"
                     /locus_tag="NK767_002132"
     CDS             6020..7747
                     /gene="ptsP"
                     /locus_tag="NK767_002132"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCH6695115.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            7911..8957
                     /locus_tag="NK767_002133"
     CDS             7911..8957
                     /locus_tag="NK767_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCH6695116.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            8974..10197
                     /locus_tag="NK767_002134"
     CDS             8974..10197
                     /locus_tag="NK767_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCH6695117.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            10286..11173
                     /locus_tag="NK767_002135"
     CDS             10286..11173
                     /locus_tag="NK767_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCH6695118.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            11259..11489
                     /locus_tag="NK767_002136"
     CDS             11259..11489
                     /locus_tag="NK767_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HCH6695119.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(11546..12511)
                     /locus_tag="NK767_002137"
     CDS             complement(11546..12511)
                     /locus_tag="NK767_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCH6695120.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         4100 a         2190 c         2566 g         3731 t
ORIGIN      
        1 taaacaagca acacgtcttt tcttttagtc agctgacaaa aaaatatttg actcttatgg
       61 tttggtggct ttttcattcg ctgaatccat gctatactga ttcagttgta gtagttgaca
      121 aaggttgtga aaaagctatc ctgcattaag taataagagc agccaagcaa aaacagcttt
      181 tcgtattttt ggcttgggtg ggcttattgc tgataggctg tctctagaac gccgtttact
      241 cggaaaaaga gactgctaca cggcttttgt cacagcccta tatgaaattg tctttcttta
      301 agagatcgga caatctgtgc ctagagaaaa tataaataat tgaatggaag aaatggagtt
      361 aggttatgaa taatccaaaa ttaaagcaac aagtggacaa tcgcagaact tttgcgatca
      421 tttcccatcc ggatgctggg aaaacaacga ttacagaaca attactattg tttggtgggg
      481 ctatccgtca agccgggacc gtaaaaggca aaaaaacagg taattttgcc aaatcagact
      541 ggatggaaat cgagaaacaa cgtgggattt ctgttactag ttctgttatg cagtttgatt
      601 atcaagggaa acgagtaaat atcttggata caccaggaca tgaagatttt tctgaagata
      661 cttatcgtac tttgatggcc gtggatagtg cggtcatggt aatcgatagt gcaaaaggga
      721 tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa acgtggcatc ccgatattta
      781 cttttatcaa caaactagat cgtgatggtc gtgaaccatt ggaattgtta gaagaattag
      841 aagagctatt agatatcgaa tcttatccga tgaattggcc aatcggtatg ggtaaagggc
      901 tagaaggtct ttatgatatc taccataatc gtgtagagtt ttatcgtccg gaaaattatc
      961 aggatgaacg attagtagaa ttggatgaag atggactgct tcccgaaaat catcctctaa
     1021 ctgaaaattc cttatatgaa caagtcttag aagaagtaga actaatcaaa gaggctggag
     1081 atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac accagtattt tttggttctg
     1141 cattgactaa cttcggtgta cagacttttt tagagacttt cgttgaattg gcaccagctc
     1201 cttatggaca caaaacacaa gatgatcaga tggtaagtcc ttatgaggaa gaattttcag
     1261 gtttcgtatt caagatccaa gcaaatatga atccagccca tcgtgaccga atcgcttttg
     1321 tccgaatctg ttctggaaca ttcgaacgag gaatggatgt ctggttggaa cgtacaaata
     1381 aaaaactgaa attaagcaat gtcacacaat ttatggcaga ttcgagagaa aacgtcgaaa
     1441 aagcagtagc aggcgatatc atcggtgtat atgatacagg caattatcag attggggata
     1501 cattgttcga aggaaaacta aaagttgctt atgaagaact cccttcattt actccagagc
     1561 tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa atccttccac aaaggaatct
     1621 atcaattagt tcaagaagga gcgattcagc tttataaaac atatctaacg gaagaatata
     1681 tcattggagc tgttggtcag ctacaattcg aagtattcca atatcgtatg ttaaatgagt
     1741 ataacgccga agtaatcatg tctccaatgg gacacaaaat cgctcgctgg atcgatccag
     1801 aagatttgga tgagaagatg agttcaagtc gcaatatctt ggctcgtgac cgtttcgacc
     1861 aaccgttgtt cttattcgag aaccaatttg cagaacgctg gtttgcggat aaatatccca
     1921 atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac aagctgccct gcatcaagta
     1981 ataagaatag ccaaacaaaa acagcctctc atattttgtt tagagccgtg acggattttc
     2041 cgtgacggct ctttttacta tattaaaatc atagaaatgt ctaatcaaaa aaatataaaa
     2101 attattttgc gtacatatga ttaaagaaag gagagtgtga cacatggaaa cagaatggca
     2161 aagacgtgaa agaaaaaaac agaaaaaaga agagatgtta cgattatgtc gattattaga
     2221 tgtaaaagac tgggacaaaa acgagcaaat tgcacaaaaa gtacgatcaa tcatccataa
     2281 agaggaaaaa gttaaaaata aagacacgcg tagaactgaa aatgaaaaga gaaaagtaca
     2341 aaagaaaaga gaaaaatcaa aaagctagaa agaaaaagag gctgagacag aagcgtttaa
     2401 ctccaagaaa taagctggaa ttcacaaaaa tttgaaaagc aattttcgtg aatttcagct
     2461 tatttccgaa ggagttgctt ctgctttcgc cgtttatacg tgtttagagc gtgagacaaa
     2521 agtgttcttt acttttgtcc cacgctcttt aattattatg ttcaaaattt gtttacacgg
     2581 ctaatgtagc gcctaattca tgttttggaa tcgagatttc aacaaggcct gccaattcga
     2641 ttactttgat ttcatcctcg tggtaaacgg cgtctttgat tttatctaag aaataatttt
     2701 tagcatcttc gatttctttc ggctggattt tacgtttgtc attgatcagc agaatttcat
     2761 tatgttcagg ggcttcagtg tagacaatag tggtatttcc ggctgtgtag accttgacta
     2821 gatgggcatc agtgttttca agctggttta atacaagacg ggaatggctg tttgttacat
     2881 ttaccaattt catggcgctc acctctttta tgtattttta attgcttgtt tctcttatta
     2941 taatttcttt tttataaaat gaacagtatt ttgacttttc tgaaaaaaga atgagacggc
     3001 atttctcaaa gaaataccgt ctcacgctat aaaactggat ttattcagcc gtttcttcgg
     3061 ctgattcttt agccaaacgt tctggtttag aagtaacgat gatttttcca tctttatcca
     3121 gttttgcttt caactcatgg atagaagggt gatcaagata aaattcagcg ataccatctt
     3181 cgatttgttc ttggatcgtg cgtcgaagag gacgagcacc catagatgga tcatagccta
     3241 agtcaactaa tttttctttg acattcgtag gaacttcgat gtgaagttgc tgtgctgcca
     3301 gcatttggtt gacatcgtct agcattagtg tgacgattgt catcaagttt tcttttgata
     3361 atgcactgaa ttcgatgatc ccgtcaaaac ggttcagaaa ttctggtgta aagtagttgt
     3421 ttaattggtt caatacagaa cgagtaacac cttcgcgtgc cgcaccaaaa ccaacgtttg
     3481 cttctacttt accagttccg gcattacttg tcataatgat gatcgtatct ttaaagctga
     3541 ccgttcttcc ttgtgcatct gtcaatcgac catcatctaa gatttgcaag aacatatgca
     3601 aaacatctgg atgagctttt tctacttcat ctaacaagac aagactgtaa ggatttctcc
     3661 gtactttttc tgttaattga ccagcttctt cgtatccgac ataacctgga ggagaaccaa
     3721 tcaatttaga gacactatgt ttttccatat attcagacat atcgaaacga atcattgaat
     3781 cttgtgaacc gaatagttcg taggcaagtt gtttcgcaag ctctgtttta cctacaccag
     3841 ttggtccaac aaataagaag gagccgattg gacgattctg tttgtttaat ccaacacggt
     3901 ttcggcgaat cgctttcgct actcgatcta cagcgttatc ttgtccgatg acatgagctt
     3961 tcaagtcatc tgctaagttt ttcagttgtg tctgttcttt ttcttttaat tcacccactg
     4021 gaatacctgt ccgttgttca acgatttttt ccatatcttt ttcggtaatc acaggtgttt
     4081 cctcttcagt gagctgacgt tctttcattt tttgaagttt attgatctga tcgcgataat
     4141 aagctgcttt ttcgaaatct tcttctcgag aagctaaaac tttttgttct tctgcttctt
     4201 gcagtttttt ctcgattgtt ttaggatcaa cgatttggat cgtcaagttt ttcttagaac
     4261 ctgtttcatc tagcaaatcg atggctttat ccggcaagaa gcgatcttgg acgtaacgat
     4321 tggataaagt tgcggctgct tcgatggcct catcagtata ttttacatga tgatagtctt
     4381 cataacgttt ttgcaaacct ttcaaaatac tgatcgtttc ttcgacagtt ggttcatcta
     4441 cccgtacagg ttgcatacga cgttctaaag cagcgtcttt ttcgatgatg cggtactcat
     4501 ttaaggtagt agcaccaacc atttgcaatt ctcctcgagc taaagctggt tttaaaatat
     4561 taccagcatc catatttcca tcaccagcag aaccagcacc gacgatttcg tgaacttcat
     4621 ctatgaatag gatcacattt tctgcttgac ggatctcttc aatcaatttt tgcatacgtt
     4681 cttcaaattg tcctcgtata ccagtacctt ggactaaaga tacgacatct aaacgaatga
     4741 cttctttgtc cattaatttt tgcggtacat caccatctac gattttttgt gctaaacctt
     4801 caacaactgc tgttttacca acacctggtt ctccaatcaa gacaggattg ttcttggttc
     4861 ggcgatttaa aatttcaatg actcgtttga tttcgtcatc gcgtccgatc acaggatcga
     4921 tatcaccttg tcgtgcttgt tctgtgatat tgattccgta ttctcccaat aaaccttctg
     4981 tattgccttg aggtggctga ccaccattga agttgttgtt tccgccaaat tgtgttggtg
     5041 gtgtttgctc ataatttcct tgctgttgca tttggcgaga catggaacgg aaaagatcgt
     5101 ccaaactgcc aaatccaaat ggatcatttt gggccatatt agtcagacct ccattttgtt
     5161 gatttttgat tttttgataa cagctctgac agtaatcgag ttgtgtccgc tgaccattga
     5221 cagtggcata caagtgaatc gttgcctcat tcttgccgca gttttgacaa agcatgaatc
     5281 attcacgtcc tttcacaaaa atgggtacat ccatatttta catctcgttt ttcatgaggt
     5341 aaaagaataa cccctagttt tttaagagat ttatgaggta gaaaaggtga aatctctatt
     5401 gaccattcct gacctttgac tcttattata ctaaaaataa aaagggaagc aagtaattcg
     5461 tttcatatga aaacaaagag ggtcaaagga aagaatgata aacgtcagga gaagagaaag
     5521 cgacaagaaa aaggaaataa acacgtttgt tataaaatgt gaagaaaagg atgaaaaaga
     5581 atccggaata atttgaatac ttttatctga aattgaatga aaactgttgt atctaacgaa
     5641 aaaaaatgtt aatctttaca gatgaaaggg tggcatttgt taggattctc tgaattacta
     5701 aaaaagaaaa ccctcacata aaaacaacac tcacttaaag gagaccgatt aatatggaaa
     5761 agaaagaatt tcacgtagta gcagaaactg ggatccacgc tcgtccagct acactattag
     5821 tacaaacagc aagcaaattt aactctgatg taaacttaga gtataaaggt aaatcagtaa
     5881 acttgaaatc aatcatgggc gttatgtctt taggtgtagg tcaaggttct gatgttacta
     5941 ttactgcaga aggtgctgac gaagctgatg caatggcagc aatcgttgaa acaatgaaga
     6001 aagaaggctt atctgaataa tggttgaaat gctaaaaggg attgccgcta gtgacggtgt
     6061 agccgttgca aaagcttacc tgctagttca accggattta acattcagta aggcaacagt
     6121 agaagatact gcggctgaag aagctcgttt ggatgctgca ttagctaaat ctactgaaga
     6181 attgcagcaa attcgtgaaa aagcagcgca aagcttaggt gaagcagaag cgcaagtatt
     6241 tgatgcccat ttgatggttc tttcagatcc agaaatggta ggtcaaatca agcaaaatat
     6301 taaagacaac agcgtaaatg ctgaatcagc gctaaaagaa gtaacggata tgtatatcgg
     6361 tatgttcgaa gcaatggaag ataatgctta tatgcaagag cgtgctgcag atattcgtga
     6421 cgttgctaaa cgtattttag cgcacttatt aggcgttact ttaccaaatc catcaatgat
     6481 caacgaagaa gtggtcgttg ttgcacatga cttgactcca agtgatacag cacaattaga
     6541 tcgcaatttt gtaaaagcgt ttgtaacaga cattggtgga cgtacttcac attctgctat
     6601 catggcacgt tctttagaaa ttccagcaat cgttgggaca aaagaaatta ctgctaaagt
     6661 caaagaaggc gatattttgg ctgtcaacgg gatcgaaggc gatgtaatca tcgatcctac
     6721 agatgaacaa aaagctgaat ttgaaaaagc aggtgcagat tacgcagcac aaaaagcaga
     6781 atgggaaaaa ttaaaaaatg cagaaactgt cacagctgac ggcaagcatt ttgaattagc
     6841 tgcaaacatc ggaacaccaa aagatctagt tggtgtccat aataatggtg gagaagcagt
     6901 tggtctgtac cgtactgaat tcctgtatat ggattctcca gacttcccaa cagaagatga
     6961 ccaatatgaa gcttacaaag ctgtattaga aggtatggaa ggaaaacctg tagttgttcg
     7021 tacaatggac atcggtggag ataaagaact accatatctg caattacctc atgaaatgaa
     7081 tccattctta ggatatcgtg cattgcgtat cagtttatct gaacaaggcg acgaaatgtt
     7141 ccgtacacaa atgcgtgcat tgcttcgtgc atctgttcat ggtaatttac gtatcatgtt
     7201 ccctatggta gctacactca aagaattccg cgcagcaaaa gcgatctttg aagaagaaaa
     7261 acaaaaattg ataagtgaag gcaaggaagt atctgatacg atccaagtcg gcatcatgat
     7321 cgagattcca gcagcagcag tcttagcaga caagtttgca aaagaagtag atttcttctc
     7381 tgtaggtacg aacgatttga ttcaatacac aatggcagca gaccgtatga acgaacgcgt
     7441 ttcttacttg taccaaccat ataacccatc aattttacgt ttgatcaaaa atgtaattga
     7501 tgcagcacac gctgaaggaa aatgggcagg tatgtgtgga gaaatggctg gagatcaaac
     7561 agctgttcca ttgctagtag gtatgggtct agatgagttc tcaatgagtg cgacatctat
     7621 tcttaaaacg cgtagtttaa tgaaacgctt ggacacagcg aaaatggctg aacttgctga
     7681 tcgtgcattg aaagaatgcg atatgatgga agaagtcgtt gaacttgttc atgaatatgt
     7741 aaaataaaca acgtatcaaa agctggtgtg gcttgtaaaa gagctacgcc agtttttttg
     7801 ttctttttta agaaaacgtt tttgtcagta agaatagaca aaaatagggc ttaagtttga
     7861 gtgacagttt gtaggctatg ttatactata taagtgaagg gggacgtaag gtgaaggtat
     7921 tattatattt tgaaagtgaa aagatgcttg caaaatctgg aatcgggcgt gcgcttgatc
     7981 atcaaaaacg tgctctgacg gaagtcggta tctcttatac actagatgaa aaagaagatt
     8041 atgacatcct gcacatcaat acatacggaa tcaatagtca taatatggtc aataaagcca
     8101 gaagagaagg aaaaaaagtt gtctatcatg cgcattctac agaggaagat ttccgaaatt
     8161 cctttatcgg atcgaaccag ctttctccta tcgtaaaaaa atatctggta ggattgtacc
     8221 aaaaagcgga ctatctgatc acgccaacac cttattcaaa acagttgttg gaaagctatg
     8281 gtatccgtgt gccgattcaa gctatttcca atgggatcga cttagagaaa tatcaccctg
     8341 atccaatgaa agaacaaaag ttcagagaat atttcaaact gtctccggat caaaaagtaa
     8401 tcatttgtgt cggtttgttt ttcgaacgta aaggaatcat tgatttcgta gaaattgcta
     8461 agaagatgcc cgaatacaca tttatttggt ttggtcatgt tcccatgtac tcgattcctc
     8521 ggaacatccg gaaaattgta aaagaagatc atcctgaaaa tgtcctgttt cctggttaca
     8581 ttcgtggaga gatcatagaa ggtgcgtatt caggtgcaga tctattcttt ttcccttctt
     8641 atgaagagac agaagggatc gttgttttag aagctttagc aagcaaacaa aacgtacttg
     8701 ttcgagatat tccagtgtat aatggctggt tagaagatag caagaactgc tatatgggga
     8761 agacaaacga agaatttatc cgactgatcc aacagattac caatcaagaa ctaccgagta
     8821 cgacacaagc cggttatcaa acagcaaaag agcgaagtat caataaaatc ggtgaagaac
     8881 ttaaaaatgt atacgaaagt gtactgaatg aaaaagtctc tagcaaatta aaaaaagtaa
     8941 atcaggtcaa ggactagttt ggaggcgaaa aacatgaaaa tcggcttttt taccgatacc
     9001 tactttccgc aagtcagcgg agttgctaca tcgatcaaaa cattaaaaca agaattagaa
     9061 agacatgggc atgaagtcta tatttttaca acgaccgatc ctaatgcgga taagttggaa
     9121 aaagatgtga ttcgaatgcc aagtgtacca tttatttctt tcaaggatcg aagagttgtt
     9181 gttagaggca tgtggtatgc ttatttggtt gccaaagaac tagaactaga tcttattcat
     9241 acccatacag aattcggagc agggatactt gggaagatgg tagctaagaa gttgaaaata
     9301 cctctgatcc atacttacca tacgatgtat gaagactacc ttcattatat cggtaaagga
     9361 aaagtgatca ggcaatccca tgtaaaatac ttgtctagat tatttgcaaa tcatacgaca
     9421 ggtgtagttt gtccaagtga acgtgttatt gatacattac gcagctatgg tgtaatagca
     9481 ccgcttcgcg tgattcctac tggtatcgac attgaaaaat tcaaacgttc tgatatcact
     9541 cagcaggata tcagcaaatt aagggagtca ctaggtcttc aggaaaacca tttgatgatt
     9601 ctttctttaa gcaggatttc ttatgagaaa aatatccaag ccttgattaa tgggtttcct
     9661 caggtcatct ccgcttatcc taacgccaga ttagttatcg taggaaaagg accttatgta
     9721 gaagagttag aggaattgat ctcagaactg cagttagaag aatttgtgca gttcacagga
     9781 gaagtggaca acaatgatgt agcactttat tataaagcgg cagattattt tgtcagtgct
     9841 tctacttcag aaacacaagg gcttacttat acagaagcga tggcagccgg aacaccttgt
     9901 gtcgtggaag gaaatgctta tttgaaccga ttgttcgatc atccttcatt agggaaaact
     9961 tttgaaaccg acgaagactt tgcaccagca cttattgatt atatttccag tgaagttcct
    10021 aaagatccaa aagttttaga agctaaactt tatgatattt catctacaca tttcggcgaa
    10081 gctatgatcg aattttatca agacatggtt gtttatcatg aagaagagat gcgaagaaaa
    10141 gaagaagaag actcgattga acgaatcaaa ataaaattaa attcatttaa aagataaaat
    10201 tcaaacattt tttatgaaaa aatccttgtt tctagcgtat actagcatag aagcaaggat
    10261 ttttgatttt taggaggtcg aaaaaatgaa acttggtttt atcggaacag gcgtcatggg
    10321 aagtgctgtc gcacgccatc tactagaagc aggacacgaa gtagctgtct ataatcggac
    10381 aaaagcaaaa gctgatcctt tagtaacaga aggagctatc tgggcagata caccaaaagc
    10441 ggtagcagaa caaagtaata ttctctttac aatggtaggt taccccaaag atgtagaaga
    10501 aatctactat ggacagtcgg ggattttttc tgcagatatc tcaggacata ttttagttga
    10561 tttgactaca agtacaccaa gcctggcaga aaaaattgca aaaacagcaa aagaaaaagg
    10621 cgcagatgcg ttagatgcac cggtatccgg tggtgactta ggagcaaaga atggtacact
    10681 gacgatcatg gtcggtggag aagaagccgt ttatgatcaa gtactgcctt tattcaaaga
    10741 atttggtacg acatttacat tgcatggttc tgctgggaaa ggtcagcata caaaaatggc
    10801 taatcagatc atgattgcag gtacgatgac aggaatgacg gaaatgcttg tctatgcaca
    10861 aaaaaacgga cttgatttaa agaaagtgat tgagacgctt tcaggtggaa gtgcagctaa
    10921 ctggtcttta agcaattatt ctcctcgtat tttaaaagaa gattacacac ctggtttttt
    10981 tgtcaaacat tttatcaaag atttaaagat tgcattagaa gaagcagaaa aaatggatct
    11041 tgtattgcca gcaactaccc aagcattgaa actatatgaa gagttggcag ataaaggttt
    11101 tgagaatgac ggaacccaag ctttgatcaa actttggtgg cctgaaggaa aaataccaga
    11161 aaaaaaatca taaaaaacga gtttttttag agaaaatcaa tgcaagtagg ctatgctttt
    11221 gatacaatat aacagaaata agaaaaggag gacctccgat gacacattca caattagtcg
    11281 caatcataaa acgactggag gctatggtcg agtcagcaga taatgaactg caagttcgcc
    11341 gttttgaaaa agaaggcgta gagaagtgta cagtaactta tgataaagcg accgatacat
    11401 ttgaattaac tgaaacagat actcatcagc aatacgaatt tgataatatc gatatcgtag
    11461 cgatggaaat ctatgactta atccaataaa ttttaggata attgacattt gaagttgaag
    11521 atgcctaaaa gaagccgaga agcaactaat ttagttgctt ttcagcttct tttttgttta
    11581 acagttcaat ttcaaactgt tctttttcta attttttgcg gtaactgatc aagatttgta
    11641 caggatcgat ttcttctttt gacaattgac gtttcaaaga taaatgagct tctttctttt
    11701 cataatagaa gtcaggagaa acgtcaccag aggtaatcat tgtttctgca agttgaccat
    11761 aacaaacgag gtagtgcttt ttctgactct catctgctgc taatggcatg ctgaaggtga
    11821 gcgatggtgt tcttaaaaac tgcttttgtt cctggatccg tctaataaaa taagtaacta
    11881 attcgatatc tttttgaagc ataccggctt tttcgaaatt caactgatag atgaaatgtt
    11941 gttgtctttt tttcaagtaa gtcagtagct ttttatttcg gccttgaaac agcccttgga
    12001 gttccttttt tttctgttca aaagtcattt cctgtaggtc aggaagctgg actcgcaaag
    12061 ctaacaaact aatttcagac aaccaaggca tttgataagt ttcttccatt atacgcaaaa
    12121 tagacggcat ttttttatag agacgaaaag gcccgtatgt tcgagccgta ggagtatctg
    12181 ttaatacaaa tcccttattg gtaatatgta cataattata attactgaaa taattcattt
    12241 gtcggttata aaaaggacgg taatgttgga tcaattgaca ctcgagcaat aaagcatcta
    12301 attccgtgtc tactacgacg aaatcaaaat ctgttatatg atgaatcata cgtaatactt
    12361 tttttgaatg ctgtttattc tgatgaaaat agctgcttac tctatttttg agccgttttg
    12421 ctttaccaac atagatgacc ttaccatctc ggtctttcat aagatagaca cccggcagca
    12481 tggggagagt tttcattttt tcttttaaca taggatctcc ttttacgctt tgaataaaaa
    12541 gactataaca aattttgaaa taatggacaa gttttaggga attctat
//
LOCUS       DAHUVV010000080        12340 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.083, whole genome shotgun sequence.
ACCESSION   DAHUVV010000080 DAHUVV010000000
VERSION     DAHUVV010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.083"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..154
                     /locus_tag="NK767_002138"
     CDS             <1..154
                     /locus_tag="NK767_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCH6695121.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(235..1218)
                     /gene="istA"
                     /locus_tag="NK767_002139"
                     /pseudo
     CDS             complement(235..1218)
                     /gene="istA"
                     /locus_tag="NK767_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1613..2455)
                     /locus_tag="NK767_002140"
     CDS             complement(1613..2455)
                     /locus_tag="NK767_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCH6695122.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2780..3196
                     /locus_tag="NK767_002141"
     CDS             2780..3196
                     /locus_tag="NK767_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCH6695123.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="NK767_002142"
     CDS             3193..3663
                     /locus_tag="NK767_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6695124.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="NK767_002143"
     CDS             3677..4450
                     /locus_tag="NK767_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6695125.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="NK767_002144"
     CDS             4464..5276
                     /locus_tag="NK767_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCH6695126.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="NK767_002145"
     CDS             5330..6295
                     /locus_tag="NK767_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCH6695127.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6432..9155
                     /locus_tag="NK767_002146"
     CDS             6432..9155
                     /locus_tag="NK767_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCH6695128.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9168..9800
                     /locus_tag="NK767_002147"
     CDS             9168..9800
                     /locus_tag="NK767_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCH6695129.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10005..10232
                     /locus_tag="NK767_002148"
     CDS             10005..10232
                     /locus_tag="NK767_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCH6695130.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10301..10567
                     /locus_tag="NK767_002149"
     CDS             10301..10567
                     /locus_tag="NK767_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCH6695131.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10557..10826
                     /locus_tag="NK767_002150"
     CDS             10557..10826
                     /locus_tag="NK767_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCH6695132.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(11005..12306)
                     /locus_tag="NK767_002151"
     CDS             complement(11005..12306)
                     /locus_tag="NK767_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCH6695133.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4031 a         1999 c         2422 g         3888 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcaa gatgcagcga ttaaatgact atctttatca actaacattg cttttgaaat
     6481 ctacgccttc agataattat tcagctcgca gtatttcgca agctctacag ttgaatagaa
     6541 gtacaatcag tagctactta aatgaaggtg ttcgtgaagg gcttttcata aaagtaaaaa
     6601 gttacccggt tttattttta catagaacag ctcttgaaga actacatatc acactgaata
     6661 actccgaaat agaatcaatt gaaagcttgt taacaatttc tcaaaaaccc gcattagatc
     6721 aagtaattgg ctctaaagga agtttgaaag aagcaattga tcaaattaaa acggcagtct
     6781 tatatcctgg aaaagggttg cctttattat tgatcggcgc tagtggatca gggaaaacct
     6841 ttctagcgaa taaaatatat gaatatgcag tagaagaaaa ggtcattcac acatcggcac
     6901 ctttcataga ttataattgt gcacaatatg tgagcaatcc agagttacta tcctctgctt
     6961 tatttggcta cacgaaagga gcatttaccg gagcaagcca ggagcatact ggtttgctag
     7021 aaaaagccga tggaggagta ttgtttttag atgaagtgca tcgcttatct gaagaagggc
     7081 aagagaaatt atttacattt atggatacgg gtgaattctc tcctatgggg gataatagta
     7141 ttcggaaaaa agccgatgtt cgattagtct ttgcaacaac agaaaatata tatacgactt
     7201 tcttaccaac ttttcttagg agattgccag ttattgttaa tctacctaga tttcaacaac
     7261 gtccgtcgtt tgaacgtctg tcattgattg atgaattttt tgtttctgag agccaaattt
     7321 tagctaagga actatctgtt tctgatgcat tgattcattt cttaatgaat agtgattttg
     7381 aaggtaacgt ggggaatatt aaaaatatta tcaagtatgc ttgcggaaac gcatatatcc
     7441 accagaagag tcagcaaaca attcaagtta aattattgga tatgccttta gagtacagtt
     7501 ataaattcaa ggagcaacta aataaaccca gaaagaaaag agcagatcga atctactatc
     7561 cagaagaaac acgacaagtg caactagaaa gaaacaatca gctttttgtt caattttttg
     7621 accaattact tactggattc tctgaagtga ttgaaaataa caaggatatt aaactatttt
     7681 tagaagaaat ggttaccaag gtaacacaag taatggatac gcttattttt caagaggatt
     7741 acgaaaaaga gcagtcttta tacgccattc taaactatca tattcgtcaa agtattgatt
     7801 ttatgcgaga aagctacggg tttgaacaag atggtaaccg ggtagtcgct ttggccaact
     7861 atctatatgt caaggataat caagtgattt ttgaaaatag tcttcaagaa aagaaaacca
     7921 aagatcagct taacgaattt tttgctaatg ttcttgaaac cccttattgg tatgcaaaaa
     7981 aaatcttaag ccttttatct gagtgtttag atcaacctgt gtatgaagag gacattttct
     8041 ttattacgct atatttttat agtttgaatg tcacgaatgt taaaacaggt attaagagta
     8101 ttgtgctagc tcatggttat tctacagcta gtagtatggc gaatgttgcc aaccgtatgc
     8161 tgaataagaa cgtttttcaa gcttatgata tgcctttaga tattacatta gagaaaattg
     8221 aagcaaaatt gattcaattc cttggagatt atcgaacaga ttcaggcttg attttattgg
     8281 tagatatggg ttcactaaac caactaggcg gtttattagt gaatcatcta aatggaccac
     8341 tgttaatcat tgatcatatc aatacaccgt tattattgga agtcggccaa caagtattaa
     8401 acaataaaag tatcaccgaa ataggagcag aaattgaaac aagtcttact ttcaaaaagc
     8461 agttactact tcccaagaaa aagaaaaaga aagccatcat tacttgttgt tatacaggga
     8521 ttggtagtgc gacccagatt caggagattt taacgaaatg cttgaaagat gcagttagag
     8581 aattagtaat tattccgtat gattacaaaa agctgttaga aaacaagaat tttgagactc
     8641 cttttcaatt atacgatgtc atcatgattg taggtacaga agatcccaaa attaatcaga
     8701 ttccatatat tggattagat cgcctgatta atgggggaag cattggcgaa tttgttcatt
     8761 acttggaaaa agagctgact attgaagtga acgatttaca aaaagaatta attttcaatt
     8821 tttcaattag taaaattgtc gaaaatttaa ccattttgga tccagataag gtgctatctt
     8881 ttgtacaaaa agcaattaaa gaattatcga agttattaaa ttttgatttt tctaataatc
     8941 agctgttttt gttgtacctt cactgtagtt gtatgattga acgaattgta cgtaaagaaa
     9001 gtgtggatga gcaagaggac attgaagaat atatgcaagc aaatggagaa ataatggata
     9061 aaattcatag cgcttttttt gagattgaaa gggaatacac aattgaaata ccagatttag
     9121 agttgcgatt attgagcgat attgttcaag gataggagtg aattcgaatg acaatgggag
     9181 taatttttga tatggatggg gtactcatag atagcgagac attttatttt gagagaagaa
     9241 tgaacttctt tcaagaactg gagatagatc caggatcaaa ccagattgat gattatgtgg
     9301 ggaaaacaga agctgcaata tgggaaacat tagttcctga ggacaaatca ttaagagagg
     9361 aactttatcc gaaatatgta gattatcgaa aaacgcatcc aattgatttc gctaaagctt
     9421 tacgaaaaga agtgaaaccg ttacttgact tcctcaagaa gcgggatata ccattggcac
     9481 tggcatcttc ttctccagct gtcgagatag agagtatgct cattcaatgt gaacttactg
     9541 aatatttttc ctttgttata agtggtgagt cattaaaaca aagcaaacca catcccgaaa
     9601 tatatttaaa agcgaaacag caattagact gtgacttctt tattgcagta gaggattcaa
     9661 cgattggtat taaatcagca aaggcagcaa atatctatac ggcggcacta aaacaaccaa
     9721 tgcaaatcga tcagagtgaa gcagacatac aaattgaaag tctacaagaa ctggaacaaa
     9781 ttgttttaga aaaaggttag tgaattaatg gaaataaggc tatatatctg tgctagctat
     9841 tttgactaac caaaaaaact tgctataatt caggtgtagt aaaaggtgct ggtaacacct
     9901 tttacgaaaa tgcttcggca gtgataatgg gattggcttt ttaagttttt tattttgttc
     9961 gcaatcccta aggtcatgtg aatgatccta gggattgcta gaatattttg caacagaacc
    10021 aaaatacgaa tagttatcaa gcagccatat tgactgcggt caatttagga ggggatactg
    10081 atactactgc tgccgtaact ggtgctcttg cgggtattct ttacgggggt gaagtgatcc
    10141 ctgaaaattg gagaaaatcg cttcaaaatg accagcttct taaaacgatc agtcagagat
    10201 tttgcgatac cgtgctatct caaagaattt gaataatgga tatgatgcaa atcttgtttg
    10261 tactaacgtt gtattgacaa aaaggatttt atttagtaca atgttagtac aaatgatgaa
    10321 cggaggtaga atcatgtctc aatcaaccat tacatttagg attcccgacg atgaaaaaga
    10381 actggtatta gaatatgcaa aagcacacaa ttcttcattg accgaacttt atcgaaatgc
    10441 tgtactggat aaaatcgaag atgaaatcga tctacggact ttacggagtg caattgaagt
    10501 ttccaaagag aaaaaagaga gtggtatttc tcaagaggaa atggagaaac tgctaaatga
    10561 attataaagt catctacatg ccgcaagccc agaagcaatt aaaaaaaatg gacagaaacc
    10621 aagctaaaat aattgtcgcc tggatcagaa agaatttaga aaacacgact gacccgcgtc
    10681 agcacggaaa aggtttgact ggaaataaga gtggggaatg gcgctatcga attggaaact
    10741 atcgaatatt ggctaatatt tgtgattctg aaattgaaat ccagatattc tcaattggac
    10801 atagaagtac aatatataaa agataaccgt ttttggaacg aagtagagtt tcgaaagtca
    10861 atgtataatc ttttgaattt tgcataatac cacaggtaga aactacccga tactatcggt
    10921 tggtttctgc ttttttatgg ctctttgtca aataggactg atagagtgag ataagaacat
    10981 ctaaggtaga attccttttc tactttagat gttttttgat tcataggtaa tcagattatt
    11041 tagtaagaaa attctggtct tcattttccg aaagcttcga aagccataag cgactcgttt
    11101 caatgctttg atgtgggtat gcagattttc tattttggca tttgaataag gcaattcgag
    11161 ggctaaacga atgccggatt catagtttat aaaagctttt tttatagaat gcagttcttt
    11221 tggaacggaa ttgggcatcg ttcttacaag tttaaagaaa gtgtccgcct ctttgtcgtg
    11281 aaacgcttga agaatttctt gatagtaaaa ataggcgatt cgtaattcgt cagagatcga
    11341 aagcagacga tccaccactt cactttcggt gagatacgcc caaccaaaat tgcgccattt
    11401 ggtccatttg tcataaacaa gcttgtcctg tcgtttgatc agaagccgag acaacgcctt
    11461 taattgtcga taatatttgg cttgtgttgg atcgtttcga tcaaagcttt tcatgattcg
    11521 tacgcgaata tcctcaaatt tacgaatgag atgtttgaca atgtgaaaac gatccacaac
    11581 gagttttgcc tttggaaaac attcctttat cagagaggca taagaaacat tcatgtccgt
    11641 cacgatgacc tttaccttca atcgtgcgcg tcgttcaaat tgcataaaat aagaaacgag
    11701 agcctttttc ttccgtgaag gaagaatatc cagtaatttt ccagtatcgc catccataca
    11761 agaaaaggag tagggaccga cttgattttt cagtgaacga aattcatcca ttaataaaca
    11821 acgcggtaac caatttcttt ttacttttgt catggggatc agagaagcga gtgtccgaga
    11881 aacggtcgac caagaaacgc cgcaatgctt agcaattgtc gtcattgctt gtttttcagt
    11941 tagctcttct aaaattttat agcgcacttg tttagcgata aagcagtttc tatcgacaat
    12001 gtaggtattc gccgtaaaat aggtgtggca gccacgacag tggtagcgtt gtttcgctaa
    12061 agcaagataa gtcagttggt tcgcacattt attaagttga atgagcgatt ttttctttcc
    12121 gttcttaacg atcatgcctt catgattcat ctgtttacac aaaggacagc gacgaatttc
    12181 tttacttaac gtcgcagaaa caacaagact cttgatattc ttaatgctct tcgattccac
    12241 atttgtgatg aaaatgtttt tatctttgat tccaagtagt agtttgatat gatgaagatc
    12301 aggcatacag aacgctcctt tgattgtgtt gtggtgattc
//
LOCUS       DAHUVV010000081        12129 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.084, whole genome shotgun sequence.
ACCESSION   DAHUVV010000081 DAHUVV010000000
VERSION     DAHUVV010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.084"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(7..564)
                     /gene="frr"
                     /locus_tag="NK767_002152"
     CDS             complement(7..564)
                     /gene="frr"
                     /locus_tag="NK767_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCH6695134.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(567..1289)
                     /locus_tag="NK767_002153"
     CDS             complement(567..1289)
                     /locus_tag="NK767_002153"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCH6695135.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1425..2306)
                     /locus_tag="NK767_002154"
     CDS             complement(1425..2306)
                     /locus_tag="NK767_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCH6695136.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2405..3187)
                     /gene="rpsB"
                     /locus_tag="NK767_002155"
     CDS             complement(2405..3187)
                     /gene="rpsB"
                     /locus_tag="NK767_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCH6695137.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3546..4025
                     /locus_tag="NK767_002156"
     CDS             3546..4025
                     /locus_tag="NK767_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCH6695138.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4242..5333
                     /gene="phnW"
                     /locus_tag="NK767_002157"
     CDS             4242..5333
                     /gene="phnW"
                     /locus_tag="NK767_002157"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HCH6695139.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5326..5454
                     /locus_tag="NK767_002158"
     CDS             5326..5454
                     /locus_tag="NK767_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCH6695140.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5458..6003
                     /locus_tag="NK767_002159"
     CDS             5458..6003
                     /locus_tag="NK767_002159"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCH6695141.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6457..8148)
                     /locus_tag="NK767_002160"
     CDS             complement(6457..8148)
                     /locus_tag="NK767_002160"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCH6695142.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8568..9515)
                     /gene="arcC"
                     /locus_tag="NK767_002161"
     CDS             complement(8568..9515)
                     /gene="arcC"
                     /locus_tag="NK767_002161"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCH6695143.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9630..10649)
                     /gene="argF"
                     /locus_tag="NK767_002162"
     CDS             complement(9630..10649)
                     /gene="argF"
                     /locus_tag="NK767_002162"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCH6695144.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10740..11969)
                     /gene="arcA"
                     /locus_tag="NK767_002163"
     CDS             complement(10740..11969)
                     /gene="arcA"
                     /locus_tag="NK767_002163"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCH6695145.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3314 a         2519 c         2193 g         4103 t
ORIGIN      
        1 attttattaa acttccaata attctttttc tttatcagca gcgatcttat ctacttcttt
       61 gatgctgtta tctgtcaatg tttgtacgtc tttttctaat ccgcgcaaat catcttcggt
      121 aatatcacca tttttttgtt gttttttata ttcatcgatc gcatcacgac ggatgttacg
      181 aatcgcgatt tttgcatttt ctgcttcttt tttcacatct ttagctaatt cttttctgcg
      241 ttcttctgtc agttgcggga taactaagcg aatgacattt ccatcattgg ttggactgat
      301 tccgatatca cttgccataa tagctttttc aacatcttga agaatgctct tgtcaaaagg
      361 tgtgatcatt aaaacacgtg cttctggaat ttggattgat gccatttggt tcaatggtgt
      421 tggcgcaccg tagtagttca cagtaatacg gtccaaaagg ctagcgttcg cacggcctgc
      481 gcggatttgc ccaagttcac gttgaagatt ttgtgctgct tttttcattt tttcttttgc
      541 ttctgtcata attgcatctg ccatttttat ttccccctta cagttgttcc gatattttcg
      601 cctaagattg cacggcgaat atttccgtgt tcatttaaat tgaagacaag cagtggaata
      661 tcattatcca tgcttagtga acttgcagtt gaatccatga cttgtaatcc tttggcaata
      721 acttccagat gagtcagttc ttcaaatttt actgcatttg catctacttt cggatcagca
      781 gaataaacac cgtctacatt gtttttagcc atcaggataa catctgcgcc gatttctgcc
      841 gcgcgtaatg ctgctgttgt gtctgtcgag aagtacgggt tgccggtacc gcctgcaaag
      901 ataacgatcc gtcctttttc taaatgacgt tctgctttac ggcgaatata tggctctgcg
      961 atttgacgca tttcaatcga agtttgcacg cgtgtcggta cacccacgtt ttccaacgta
     1021 tcctgtaaag ctaaggcatt cataactgta gctaacatac ccatgtaatc agcttgagca
     1081 cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga tatttccgcc gcccacaacg
     1141 attgccattt caacgcccag ctcgtgaact tctttgattt cttggatgat ttctttgatt
     1201 actggtggtt tgattccaaa tcctgcgtcc ccagctaaag cttctccact caattttaag
     1261 actacacgct gatatttcgg tgtgaccatg ttcattcctc caatattttc tattgccatt
     1321 ttaccatact tagatgattt cgacactcta agactggttt ttcttgtttt gcttaaaaaa
     1381 agggagcgca caaaccgtgc gttcccgtct gttattcaac agaattattt tttcacttgg
     1441 ctcataactt cttctacaaa gttatcttcg cgtttttcga taccttcgcc aacttcgaaa
     1501 cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt atttttcaac agtcatatca
     1561 ggatctttaa cgaatggttg gtccaccaaa gcgatttctg ctttgaattt cttcaagcgt
     1621 ccttctacca ttttttcaac gatattcgct ggtttgcctt cgttcaatgc ttgttctgta
     1681 agaacagttt tttcatgttc taattcagct tctggaattt gtgattcgtt tacataacga
     1741 ggattgatag ctgctacgtg catagctacg tctcttgcaa ctgtttcgtc agttgttcca
     1801 tctagaacag tcaatacggc aatacgtccg cccatatgca agtatccacc aaatgcagca
     1861 ttgtcgtctt tttctacaac ttcaaagcga cggaagctga ttttttctcc gataacttga
     1921 gttgcttcga tcaagtcaga ttcaattgta cctttatctg ttttgatttt cattgcagct
     1981 tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa tttcttttac taaatcttgg
     2041 aacatttcgt ttttagaaac aaagtctgtt tctgagttta cttcaacgat tgctgcagtg
     2101 ttacctttca cagcaactga agccaatcct tcagctgcaa tgcggtcatt tttcttcgca
     2161 gcctttgcca ttcctttttc acgtaaaaga tcaactgctt tttccatgtc gccttctact
     2221 tcaactaatg cttttttcgc atccatcatt cctacgccag tcatttcgcg tagttctttt
     2281 accattttag ctgtaacgtc tgccatgttt aactcctcct gtgtgttttt ctttttaaaa
     2341 aaagctgctt caaaggagag tgaggcttgt cgcccagcct ttgaaacagc tccatcattc
     2401 aaaattattc tgctgaagaa ttgtcgcctt caacaacatc tacgatttct tcgattgatg
     2461 ttgctgtgtt ttcttctgtg aaatcttctt caactacttg atcttcacct tggtttcctt
     2521 cgatgaaagc atcagccatt tttgaagtaa tcaatttaac ggcacgaatt gcatcgtcgt
     2581 ttgaagggat tactacgtcg atttcatctg gatcgcagtt tgtatcaacc atagctacga
     2641 ttgggatatt taatttgtgt gcttcttgaa cagcaatacg ttctttacgt gggtcaacga
     2701 tgtacattac atctgggatt cttggcatat cagcgatacc gcctaagaat ttttcaagac
     2761 gttcacgttc tttgttcaaa ccagcaactt cttttttagg tagaacttca aaagttccat
     2821 cttcttccat tgcattgatt tgtttcaaac ggctgatacg tttttggata gtatcccagt
     2881 ttgtcaatgt tccgcctaac caacgatggt taacgaagta ttgtcctgca cgagttgctt
     2941 cttctttgat tgcttcttgt gcttgttttt ttgtacctac gaataatgct acgccgcctt
     3001 cttcagcaac gctcttcatg taatcataag ctgcatctac taatttaact gttttttgca
     3061 agtcaatgat gtagattccg tttctttctg tgaagatata tttcttcatt tttggattcc
     3121 agcgacgagt ttggtgacca aagtgtacgc cggcttctag taattgtttc atagaaatta
     3181 ctgccatgtt tgtttcctcc gatttggttt tatttttcct cttcttatct tctaccttgc
     3241 cggcaaactt tttcaagcac ctaccgacaa tccaataaga atgtggattt gctgatgcat
     3301 aaagcaccct cagctattat acagacaaaa gatccgtatc gcaagagcaa atatactttt
     3361 ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa ttcaagaaaa acagtagatt
     3421 tctctttgtt tctttattat cctttttcca taaaataatt ttaggtataa aagacgaata
     3481 ttattgggca atccttcttt tttttgttat gatacatgtg aaatctttcg taaaggagaa
     3541 agaaaatgcg caaagctcaa agacatttgc tgattaaaca agtcattgaa gaattcccga
     3601 tccgtacgca agaagaacta ttaaacaaac ttgggcatta tggcgttacc gccacgcagg
     3661 caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa ggcacctgat gaaaatggcg
     3721 tttcccgctt cgtattgttt cgcgaaaaag gaactcctga gactaggact gaggaagagg
     3781 atcggctggt tcaaatgatt gaagacattg tattgaaagt cgaacgagtc cactttttga
     3841 cgatcgtcaa taccttgcca gacaatgctc atttatttgc atctgtttta gatgagatca
     3901 aacctccctc tattgtcagt acaattgctg gattcgatac gatcatggtc atctcggaaa
     3961 atgaggagga cgcaaaacga gtagaagaat ttcttcacca tccaactgaa gtatcacttt
     4021 tttaatcaga gcacgtattc ctaatatgga atgcgtgttt ttttattttc aatattttcc
     4081 ttattgaata aacctttaca ctctttacaa aaatacatca ttttttattc aaatttttca
     4141 acaccttgat tgaatcacaa taatcatcat ttatattgga tgttaacaag agcagcttct
     4201 tgctctttgt gtatttcaaa aaagaaatga aaggaaatca tatgtataaa ttattaactt
     4261 ctggaccatt gacgacttct gaaacagtga aaaatgctat gctgatcgat cattgtactt
     4321 gggatcaaga atacaaagaa attacacaat ggataagaaa aaaattattg gatcttgctc
     4381 aagtatcgga aggaatatac acaacggtct taatgcaagg tagcagctcc ttcggggtag
     4441 aatcggtatt aagttctgtg atcaggccag aagatactgt actgatttgt tcaaacggcg
     4501 catatagaca acgaatgatc aacatgtgcg aatgtctgaa actgaactat accgtttatg
     4561 aggtatcgga acatcaaatc ctccaagctc atgtgattac acagctgatt gaagagaatc
     4621 cgtctatctc cgcactagca atggtccata gcgagactac ttccggtatt ttgaataatt
     4681 tggaagagat tgctgctgtc acacaagaaa gagatctttt gtttatagtg gacgcgatgt
     4741 caagctttgg tgggataccg atttgtgtag ccgaactagg gattgatttt ttgatttcca
     4801 gctcaaacaa atgtattcaa ggtgttccag gcttttcttt tgtacttgct aataaagcgg
     4861 aactggaaaa acgaaaaaat gttagccgaa gtttgtcact tgatctatat gaccagtacc
     4921 gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc ccctacccat acggttttgg
     4981 cttttcaaca agcgctgaag gaactagagg cagaaggagg aatcgaaact cgatattcac
     5041 gttatctcgc caataatcat gtgctacgac aaaaaatgac agagatgggg ttccagcctt
     5101 tcattcaaga atatcagggg ccattcatca cctcttttct ttaccctaag gactggcgat
     5161 ttgattttaa tcatttttat cagtatttaa agcaaaatgg atttgtgatc tatcccggga
     5221 aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg cgagatctat ccggaagata
     5281 ttttggaact gacagcaatt atcaaagact ataaggagaa cgttaatgat taaaactgta
     5341 atttttgatt gggcaggaac aactgtggac tttggctgta tggctcctgt ccacgctttt
     5401 agaaatgctt ttctagaaaa aggaatccaa ctgactgaca aagaaatccg ctaaccgatg
     5461 ggaaaattaa aatgggatca tatccaacag cttctttccc tcccttctgt caaaaaacaa
     5521 tggatagcta tttatggaca actacctcag gaaaaagagg tggaggaatt gtatcaacaa
     5581 tttgaacatt atctgtttaa agatttggtc aagcattcta ccttgaaacc cgacacattg
     5641 gaaacgatcg aacggttgag aaacgctggg atcaatattg gttccacgaa tggatttaat
     5701 gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag aaaggtattc accagacttc
     5761 atggcaactt ttgaagacgt cgaagggtat ggtcgaccgt ttccttatat gatccataaa
     5821 aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa tcaaagtcgg agatacggtc
     5881 tcagatatcc aggaagggaa aaatgccgga gtgttgacag taggtgtaat tgaaggtagt
     5941 tcgctaatgg ggatttctta tgatgagtac caaaaattag agatggggaa aaagatcgtt
     6001 taaaacagtc tgttgctctt agattccaac aagccggcgc tgatatttgt gtaaacaatt
     6061 taaaagagtt aacagaatac attttgtacg ctgacaagaa tccagaatga cttttataca
     6121 aaataagagg ttgtgaaaaa gctgtcctgt atcaagtaat aaaaacagcc aagcaaaaat
     6181 agctcctcat atttttcgtt tggttaggct tattaccgca gatgcaagct tttgaacacc
     6241 gtttattcga aaaaaaaaag ggagctgtga cacgactttt gtcacagtcc ccttatttgc
     6301 aaacaaacag tgtttaagag acaacctctc cactaaacgg tgttcattca gctgactctc
     6361 ggtatttgct caaaaaacga taaaatttga aaagctattt tctcttttgt ttctctaata
     6421 ctcggatcag aacgctgatt tcacattctc tttttcttac atcttatctg gtgcatgaag
     6481 tcctagcaaa cgaaggtctt ctttcaataa tacagtcact gcataaacta gagctaagcg
     6541 tgcttctttt tgttcatcat cagctaaaat cttcgtatga gcatagtatt tgttgaatgc
     6601 ttgtgctaat ttgattgcat gtttcgcaat cactgacggt tcgtattttt ccccagcaga
     6661 caagaccgtt tcagggtatt tttgaactaa tttaaccact tcccagctgg tatcgtcatt
     6721 caatgcatag tctgctgttt ctgaagggac aaatcctgct ttttccaaca ggctgactgc
     6781 acgagcatgt gtatattgca cataaggtcc agtttctcct tcaaatcgaa caacttcttc
     6841 caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa tcgtggaaaa tgactgcccc
     6901 aaccccaact tgtttagcta cagcatcttt gttttccaaa tcagggtttt tctcgctgat
     6961 ttgttcttta gcagattgga ttgcttcatt taatacttct tctaataaga cgatctttcc
     7021 tttacgagtt gataattttt taccaccttg cgtgatcaaa ccaaatggaa tatggtgcat
     7081 gtcgtcagac cagtcaaagc ccatttcctt caagacagct ttcaactgtt taaaatgata
     7141 gctttgttca ttccctacta catatagaga ttgtttgaag tcgtaagtac gtttacggta
     7201 caatgcagca gctaagtcac gtgtaatgta tagtgtcgca ccgtctgact tcttaatcaa
     7261 cgctggattc aggtcataag cagataggtc aacgatctct gcacctttat cctcgttcag
     7321 caaatgtttt tcttctaaaa gtttgacgat ttcatccatc ttgtcattgt agaaagcttc
     7381 accgttcaac gaatcgaaac gtacttccag cagatcatag attttattga attctttcat
     7441 cgattcatca cggaaccatt gccataattg aatagcctct ttatcgcctt cttccagacg
     7501 tttgaaccaa gcacgagctt cttcatttaa ttctggttct gtttcagcta cttcatggaa
     7561 ttggacgtat aaacgaagca attcattgat tggttctgct ttaacagctt cttctgtacc
     7621 ccattttttg taagcgacga tcagttttcc aaactgtgtt ccccagtcac ctaaatggtt
     7681 gatgcggatt ggctgataac cgattttttc cataatgaaa ccgatcgaat taccgataac
     7741 tgtcgaacgc aaatgtccca ttgagattgg tttggcaata ttaggagaag acatgtcgat
     7801 tggtactgtt ccttgattcc cgatgttgct atccccataa tgttcttttt ctttcactac
     7861 ggtttgcaaa acttttttgc tgataagttc tttattcatg aaaaagttca agtaaggtcc
     7921 gacaacttcg attttttcaa agttggcaga atcgattttt tctgctaaat ctgcagcgat
     7981 ttgctgtggc gcttttcggt aaacttttgc taatgaaaag gctggaaatg caacatctcc
     8041 gtgttcagct gattttggat tttctagtaa ttgctcgact tgttccagtg ttaaatcatc
     8101 tttgacagcg tcatagacag cttttgcgac aagatcttta ttattcatct ttattcctcc
     8161 taaagtgttc ccttgattgt taaacaaaaa aaatctctag cgacggcata cttagtatgc
     8221 catcactaga gacgagattt cccgcggtac cactccaatt gtcctaaaaa aggaccagct
     8281 ccatctgata tcggtagacg accgtctgtt taaaacagaa ttctccaagt gcgcttcgtt
     8341 gtatcttctt cattcggctt ccaccacttc cgaattcgct agccaagaat gatacaagta
     8401 ctctcttgtt caacaaatct tagtcttatt aaactttctt gaattatatc agaaaaaaaa
     8461 acgaaaggct agtcgtttca taaaaaaaaa atccagatgg cctaaaggag aagaaatacc
     8521 acctggatag ctataaatca tttcgtaaat acgaaagact taacaaatta atcagctacg
     8581 ataatcgttc cttcttcaga agctaaaaga ttctcgatat tttctaaaga agtgatgatt
     8641 gccttagcgt ttggttttgc ttcaacaaat gcaatagctg cttctacttt cggaagcatg
     8701 cttcctggag cgaattgctt ttcatcaatg tattgtttca tttctgaaac agtaacagtt
     8761 tctaatttct tttgatctgg tttttggtaa ttgacataca cattgtctac tcctgtcaat
     8821 acgatcagca aatccgcatc gatgatttcg gctaattttt ctgaagcaaa atctttatcg
     8881 attacggctt ctcgtccttc taatccagta gctgtttcta caacaggaat cccaccgcca
     8941 ccaacagaga ctgtgataac tccttgatcg accagcgttt cgatgacacg tgcttcttta
     9001 atcgagatgg gtttagggga agcgactact tttctccatc cacgtccagc atcttctttg
     9061 aatgtcacag tggagtcagc attcatttgt tctttggctt cttcttccgt gtagaatgga
     9121 ccgactggtt tacttggatt tttgaatgaa ggatcatttt catccacaat aacttgagtg
     9181 actaaagaaa ccacatcttt atcgatccct ttttccttca gtacttcgcc catggcgttt
     9241 tgtaaccagt aaccgataga tccttctgtc attgcaacac atgtatctaa aggcattgcc
     9301 ggtgttttct cagaatcagc agcttgctgc tgaatcaata ggttaccgac ttgcggacca
     9361 tttccgtggg agatgatcaa ttcatctcct tgttcgataa atttcactaa atactttgct
     9421 gtttccatta acgcttcttg ttgggctttt gcacttgcat ccgtagacaa gatggcattg
     9481 ccccctaaag cgacaactac ttttcgtttg gccatttcat tcgtcctttc ttcagttcta
     9541 ccgaattttc tagataacaa aacgagccag cgttttgtga ttttccctca atgctgactc
     9601 gttttttcgt gagatgaatg tggttatgct taaacgcgag ggataaacaa gttacctaat
     9661 gttgctgcca tgatcgcttt gattgagtgc atacgatttt ctgcttggtc aaattgacga
     9721 gcatatttgc tgcggaagac ttcatctgtt acttccattt cagtgatgcc aaagcgttct
     9781 ttcatttgtt caccgtaaac agtgtttgta tcatggaaag ctggcaagca gtgcaagaag
     9841 atcaaacgat ctgtgttgtg tgttttttct accatttcca tattgatttg gtaaggtttc
     9901 aacaatttga tacgttcttc gaatttatct tcttcaccca ttgataccca tacatcagaa
     9961 taaaggacgt ctgctccgtc tacaccttta tcaacatcgt cggtaatcat caattgtgca
    10021 ccggattttt cagcaaagcc ttctgccatt ttgacgattt cttcttcagg ttgtaattct
    10081 tttggtgcaa cgatacgcat attcgctcct aaaattgcac ctgtgactaa taaagagtta
    10141 gccatattgt ttcgaccatc tccgcagtaa gcaacagtga taccttctac tgttccaaag
    10201 ttttcttgga tagttaaaaa gtcagcgatc atttgtgttg gatgccattc atcagtcaag
    10261 ccattccaaa ctggaacacc agagaattct gctagttcct ctaccatttt ttgactgaat
    10321 ccacggaatt cgattccgtc aaacatacgt cctagtactt tagctgtatc ttctgtggat
    10381 tcttttttac ctaattggat atcatttgca cctagatatt caggatgtgc acctagatcg
    10441 atcgctgcag tagtaaatgc agaacgagta cgtgtagatg ttttttcaaa taaaagcgca
    10501 atattttttc cttctagata gtgatgtggg ataccacgtt ttttcaagtc tttcaagtgt
    10561 tctgaaaaat cgatcaaata ttgtaattct tcttttgtaa agtctttttc tgctaataag
    10621 cttcttcctt ggaatactga ttctttcata ttaaacattc tcctttagat tgtttttatt
    10681 taagattaaa gaggaaatcc cgacggcagc tgacagtaag actgccgtca ggaaacaaat
    10741 tacttcaagt cttcacggac aagtggttgg ctcatgcagc gtggaccacc acgacctcgt
    10801 gacaattcac ttgaattgat ttctaacact ttgatgccat agctacgaag caattcgttc
    10861 gatacataat ttcggttata agtgactaca acacctggag cgattgccaa tgtgtttgat
    10921 ccatcattcc attgttctcg aggtgcaacg atctcatctc cattaccagt tgggatcagt
    10981 acaagatcat ctaatccaag agcagctttc atcgtttcgt gtaagttatc tgaatgagta
    11041 atcttgatat catcaccatc tggttcgatt gtaaagacat ccatcttacc gtctttactt
    11101 tgaattgctg gatggatagt gaatttatcg tgatctacca ttgtaaatac ggtatccaaa
    11161 tgcatcattg cacggttgtt cggaattttg attgccaaca ctttttcaaa tcctgaattt
    11221 tttgcgaata ggtggcgtgc taatttttct aaagctttcg cattagttcg ttgagaaatc
    11281 ccaacagcta ctactttatc gcttaagatc aattcgtctc cgccttcgat atgatctgga
    11341 ttttcacgat ccaaccatac ttcgattcct ttatttgcaa atcttggatg atgtttcaaa
    11401 atatattctg taaacatcga ttcacgacga cgtgcttcaa aagtcatctt attgactgtc
    11461 atcccatttc ccattgatgc ggaagggtcg cgtgtaaagt acaagtttgg cattgggtcc
    11521 atgtaaaatg gatagtcatc gtctgctgac agatcatata gactgctaga acgtacttcg
    11581 atatcttttg tacgaacccc agccatgatt ttatctacca tttcttgcgt ttccattgat
    11641 agaaggaact catgaagacc ctctcttaca gcattcgatg caatatgaga ttcatccaac
    11701 attttattca agaattgttc tttgacattg cctgcgtcaa tcgcttctgc cgctaatttt
    11761 tccaagtaaa gagtctcgac gccttcattt tgcaatgtct tcgcaaagtt gtcatgttct
    11821 tcttgagcaa tcggaagata tggaatatca tcgaacaaca gacgatccat aatatctggt
    11881 gtcaaatttt ccacttcttg tccaggccgt ttcagtaaaa ctgtctttaa tttccctatt
    11941 tcagagaaaa cgtgaatagg tttatccatg ttgtgttgcc tccttctttt tgattacatg
    12001 tataggatat cctccttttg taatcacttt cataattttg tctcattcaa aatgagacat
    12061 tcctcacatt aatattcgat aattaaataa ttaaacgttt ttctattttt aagcgtaatt
    12121 tattcattt
//
LOCUS       DAHUVV010000082        12068 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.085, whole genome shotgun sequence.
ACCESSION   DAHUVV010000082 DAHUVV010000000
VERSION     DAHUVV010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.085"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(116..703)
                     /locus_tag="NK767_002164"
     CDS             complement(116..703)
                     /locus_tag="NK767_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695146.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            878..2200
                     /locus_tag="NK767_002165"
     CDS             878..2200
                     /locus_tag="NK767_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6695147.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2355..3131
                     /locus_tag="NK767_002166"
     CDS             2355..3131
                     /locus_tag="NK767_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCH6695148.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3124..3693
                     /gene="rnmV"
                     /locus_tag="NK767_002167"
     CDS             3124..3693
                     /gene="rnmV"
                     /locus_tag="NK767_002167"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCH6695149.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3686..4570
                     /gene="rsmA"
                     /locus_tag="NK767_002168"
     CDS             3686..4570
                     /gene="rsmA"
                     /locus_tag="NK767_002168"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCH6695150.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5021..5650
                     /locus_tag="NK767_002169"
     CDS             5021..5650
                     /locus_tag="NK767_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695151.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFGLIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5774..6883)
                     /gene="ugpC"
                     /locus_tag="NK767_002170"
     CDS             complement(5774..6883)
                     /gene="ugpC"
                     /locus_tag="NK767_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCH6695152.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7092..7514
                     /gene="mgsA"
                     /locus_tag="NK767_002171"
     CDS             7092..7514
                     /gene="mgsA"
                     /locus_tag="NK767_002171"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCH6695153.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            7837..8373
                     /locus_tag="NK767_002172"
     CDS             7837..8373
                     /locus_tag="NK767_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCH6695154.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8413..10176)
                     /locus_tag="NK767_002173"
     CDS             complement(8413..10176)
                     /locus_tag="NK767_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6695155.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10179..11894)
                     /locus_tag="NK767_002174"
     CDS             complement(10179..11894)
                     /locus_tag="NK767_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6695156.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         3771 a         2222 c         2461 g         3614 t
ORIGIN      
        1 atcactttat gttcaattag tttgtgctct tatatgtgtg attgaaaata tagataaaaa
       61 aacaatcatc ggctttgact tcgatgattg ttttctttat tttgtaaaaa tcctatcact
      121 tgattttatg gtaacctttt atttttgtct tcaaacggct tggtaagaga gcagtaagca
      181 gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg tccgatacca taatgttgat
      241 agagctttgt caacatcacg tgtaacaaga tcaaccctaa aagcaaggag ccagctataa
      301 accaatgatt atcccaatgc atgacttcac taagtgccat cactccaaaa ggatagaaat
      361 aaaaatagat cccgactaag gtcatccgcc agttctttgt aaatgttccg atattcaata
      421 aaaagaaaat cgctgaaagc aaaataccga taggcggaaa aagcccaacc aaaaaggaat
      481 acaacatccc ccacaagatc atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca
      541 aagtcagcaa tattagtttt tgtgataacg ttgcaacagt cattaatcca aataaaatta
      601 atgtgaacca atgccaatgg gactgctgtt gttctttacg ggaaaaatcg acccggttgg
      661 ataaataatc tgctaatttc ggataagaac gcttaaaatc cataaactgt tcctccttcg
      721 tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga
      781 gtttttcact gaaagtggag aaaaaagaat ggtaaattaa aatattctca gttatactga
      841 aaattatacc aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac taaaacgaga
      901 aatcaatctg tttggcgcat tagctaccgt tatgggaacc gtgatcggtg caggcgtctt
      961 cttcaaaact gctgctgtta ctgctagtac tcagtctaca agtttgacat tgctagcttg
     1021 gcttttgggt ggtttcttaa cgatctgtgc tggattgaca gtggctgaat tagctacagc
     1081 aatcccggaa actggtggag ctgtaaaata tattgaagct gcgtatggaa aattgcccag
     1141 ctttttatta ggatgggcgc aaagcttgat ttactttcct gctaatattg ctgcattatc
     1201 gattatcttt gcgacacaat tgacgaactt attacaatta tctaccgatt atcttttgtt
     1261 gattgcagtg atcaccgctg tttctgtgac agggttgaat ttgttaggga caaaagtcgg
     1321 tgcttctgta caatcagtga cgttgattgt taaattaata ccaattgctg tgattgtcat
     1381 atggggatta ttgaccccag gacaaggaac tgtccagtta tttccaatcg aagctggaaa
     1441 agatgttact tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt ttgcttatga
     1501 cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa aagatttgcc
     1561 taaagcaatt attcttggtt tgagttttgt aacagtcgtt tatctattga tcaattttgt
     1621 ttttttgaaa actttgccga ttgatcatct tgccggtaat ttgaatgcag cttctgaagc
     1681 atcagacgtc atttttggag gaatcggtgg aaagctggtc actattggga tcttgatctc
     1741 ggtatacggt gcattgaacg gttatacact aactgggatc cgcgtgcctt atgcgatggc
     1801 tttagaagac gagctacctt tcagtaaaca gctgacaaag ctttcaaaga agtttacggt
     1861 tccttatgtt cctgcggttt tccagttagc agtagcttgt attatgatga gccttggttc
     1921 tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt tactgatttt
     1981 tatcggtgtc tttattttaa gaaaaaaagc gccggaattg ccacgtccat ataaagtacc
     2041 actttatcca atcgttccga ttattgcgat tctcggtgct attttcattt taggtatgac
     2101 gatgatgacg caaacgaaat tagcattgat cggtataggt gttaccttga taggtatccc
     2161 agtctattat caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa caacagacag
     2221 aaaaagtacg aactaggtcc tctagatctt cgtgcttttt ctatttcaat aaaacaagtt
     2281 cttctatttt gatttttgct acttctgcaa gtagtgctta tgtgctaaag taagaatgaa
     2341 aaggagagat aaacatgata tttgattctc atacccattt aaatgctgaa cagttcaatg
     2401 aagatattcc agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg
     2461 tgggcttcga tacgccaacg attgaaaagt ctttgcaatt aagccatgac tatccaaata
     2521 tatacagtat tatcggctgg catccgacag aggctggcag ttatacgaaa gaaatcgaaa
     2581 ataaattaca agaacaattg actttaccaa aggttgtagc attaggggaa attggcttgg
     2641 attatcattg gatggaagac ccgaaagaag tccaagataa agtatttcgc agacagattg
     2701 ctatcgcaaa agaaatgaac ctaccaatca gtattcatac tagagaagca cttgaagaca
     2761 cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg tggaatcatg catagtttca
     2821 gtggtgatta tgaatgggca aaacgatttc ttgacttagg gatgcatatt tcatttagcg
     2881 gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat gtaccaatcg
     2941 aacgattact tgtggaaaca gatgcccctt atctggcacc agttcctttt agaggaaagc
     3001 gaaatgaacc aggctatact cgttatacag tagaaaagat tgctgaactt cggaatcttc
     3061 cgacagaaga agtggctgcc caaacatgga caaatgcgca ccgattattt ggtctaagcg
     3121 aacatggata agatgaagat ccaagagatt atagtcgttg aaggaaaaga cgatacaaga
     3181 aggctgcggg aagtagttga tgcagatacg atagaaacaa tagggtctgc aatcaatgaa
     3241 gagatcctga tgcaaattga acatgcccaa gagactcgtg gagtgatcgt ttttaccgat
     3301 cctgattttt caggagaaaa aataagaaaa atcattatgg aagctgttcc tcaggcaaaa
     3361 catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag
     3421 catgcaagtg atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc
     3481 caaacggctg tttctgaaat cactagacaa gaccttttag actatggttt aatagctggg
     3541 gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac
     3601 ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga actcgcccaa
     3661 gcaatgaaaa tagtggagga acaacatgag tgaatatcgt gaaattgcga caccctctag
     3721 aacaaaagag attttattga aacatggttt ttcatttaaa aaaagtttag gacaaaattt
     3781 tctgactgaa ccaaatattt taaggaaaat cgtagaaact gcgcaaatag atgaacatac
     3841 gaacgtgatc gaagttggac caggaatcgg tgcattgacg gaacaattag ccaagcatgc
     3901 aaaacaagtc gtagcatttg agatcgatga tcgtttgatc cctgtattgg ctgatacgat
     3961 gcaaccctat gacaatgtga ctattgtaca tcaagatatt ttaaagaccg atttgtcaac
     4021 agctgttaga gaaacatttc atgaggagct tccgttaaaa gtcgtagcga atttacctta
     4081 ctatattacg acaccgatca tgatgcattt tcttgaatca gatttggttg tggatgaact
     4141 agtcgtcatg atgcaaaaag aagtagctga tcgtatctct gctgaaccag gaacgaaagc
     4201 ttatggttca ttatcgattg ctgttcaata ttatatggaa gccagtttag cattcatcgt
     4261 accaaaaaca gtatttgtcc ctcagccaaa tgtagattca gcgattttga aactgacaag
     4321 aagagatata ccggcagtcg aagtgacaga cgaaaaagca tttttccgat tgacaaaagc
     4381 ggcatttcag caaagaagaa aaacgctttg gaataacttg cagcattcgt atggcaaaga
     4441 tgaccagaca aaagcatggt tagcaaaaag cttggaaaca gctggaatcg atccaaaacg
     4501 tcgtggggag actttgtcat tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa
     4561 tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa
     4621 taagaacagc caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc ttattaccgc
     4681 agatgcaagc ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa
     4741 gtcacaagga acaatttcaa aaaacagctt ctcatgtttt tgagttagtt ggtcttatta
     4801 cttgatgcaa gcttttgacc actattatgc cgaacaagga gatgtgacac aaattttgtc
     4861 acatctccta tttttgtttc ttgatcaaaa aattcttata cttgatactc acactcattt
     4921 tgtatggatt tagaaaagaa tatgtgtctt gacagttatt agtaagtgag ttatactaag
     4981 tcgacattta tgtgtcaaga catattagga gagagaaaaa atgagaaaaa attcagttcg
     5041 aaaactaacg atttctgctt tattgattgg gatgggggtc attatcccaa tggtcatgcc
     5101 taaaatcatg attccaccag cttcctttac tttggcgtct catgtgccat tatttattgc
     5161 gatgtttttt acaccaggag ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt
     5221 tttaacaacg ccggttatca tcgcattgcg agcattgtca catcttgtgt ttgggttgat
     5281 tggggcttgg tacttgcaaa agcatccgag tatcgtttta aaaaatggac agtttacttt
     5341 tgctaattgg cgtttccaag ggttcaattt tgtgatcggt gtgattcatt ctttagcaga
     5401 aatgctagta gtcagcatct tttactttat tggtaatatg cctgaaacat attattctca
     5461 aggatatttt tatacagtat tcatcttgat ggggattggt gggcttatcc acagtttagt
     5521 tgattataat attgcttatt ttatagcagg aacactcagc aaatattttg atatccccgt
     5581 atttacggca gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg cgaagaaaac
     5641 gacatattag acatgatctc tcaaaaaaaa aactctcatc tagtaatgga taataaatcc
     5701 tctaagatga gagttttttg tcctaaaaag agctgcacca acatcttctg gcacagctct
     5761 aacagggggc aatttatgga agtttgatga cttgttctgt ttctttatca aagaagtggc
     5821 ctttgctgat gttgaaggca aggtcgacta tttcgcctgg tttatggaaa tctcttgcat
     5881 ctacttttga gatgaactca gtagaaccag tttttgtgta aagcatcgtt tcagctccaa
     5941 gtaattcgga aacgacaact tctgatttca ctacggcttc aggtgaagct tcaagggcta
     6001 cttgttcact gtggatgtct tctggacgaa taccgaagat aacttctttt ccttcataac
     6061 ctttttcaac taatactttg ttttttcctt caggaatacg aagcttaagc ccatgtccat
     6121 ctgtgataac accatttttc aatgtcacat taaagaagtt catggcagga gatccaataa
     6181 agccggcaac gaacatattg acgggtgtat cataaacttc tttcggtgta ccgatttgtt
     6241 ggataaatcc gtctttcatg ataacgattc ggtcagccat agtcattgct tctgtctgat
     6301 cgtgagtaac atagatggtc gttgtttcaa gacgctggtg aagtttggca atctcagcac
     6361 gcatggctac acgtaattta gcatctaaat ttgataatgg ctcatccatt aagaagactt
     6421 ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca ccagataaag
     6481 cagctggttt tcgtttcaag tattctgtca gacctaagat ctctgctgca ttgtccacac
     6541 gttttttgat gtctgcttta tcatatttac gtaatttcaa gccaaaagcc atattatcaa
     6601 aaacagtcat atgaggatac aatgcgtagt tttggaaaac catggcaata tcacggtctt
     6661 taggtgccac gtcattcatg acttttccgc cgatatttag ttctccttca gagatatctt
     6721 ccagtcccgc gatcattcgc aaagtagttg actttccaca accggaagga ccaacaaaaa
     6781 cgataaattc acggtctttg attgatagat taaaatcagt tacagaataa ttttctgcat
     6841 tgtcatattt tttatatacg tttttcagag ccatttctac cataatacgt tccacacttt
     6901 cttttttagt tttattaact atttaaagta taaatgaaag cgttaactaa cttcaatgtc
     6961 atgttgaaca agaatttttt cttttttttg tcaatgtgct agttttaagc tttaataaat
     7021 atccataaga aataaaaact gatacactgg agtaaataag tatgttaaca atatcaaaag
     7081 gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct atgatggtga
     7141 aattagcaac agcgtataaa acaatattgg aaaaacatga actttttgct accgggacga
     7201 ctgggcagag gatcacggaa gcaaccggct tgccgatcca tcggtttaaa tccggaccgt
     7261 taggcgggga tcagcaaatt ggagccatga tttcggagga tcagttagat ttggtgattt
     7321 ttcttcgaga cccgctggct gcacagcccc atgaaccaga tgtaacagca cttatccgtt
     7381 tatctgatgt ctatgagatt ccgttagcta cgaatatcgg aacagctgag gtgctactaa
     7441 gaggactgga agccggcttc ctagattttc gaagaatcgt ccatgaaata gacaagcatc
     7501 cactttcatt ttgatggata agcgtatgga aaaataacgt ttaatgttcg cctatatgta
     7561 gctataaaag ctaaacaaac tgataatatg cgatataata cgaacgtatt ggataatata
     7621 aacaataata ttagtttttc gtatttgcag taaatgtgaa aatatgctat agtttctgtg
     7681 ggaaaagatc ccaaaaataa tacagagtag aaaataaagc gttaagtgct aagaggatgg
     7741 gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat
     7801 gatacgatgt agcagctctt aaaggagatg ctaaaaatga agaaacaccg gttagacgca
     7861 cgtatgattg ctattatggg cttattgatt gcattgatgg tcactttgtc ccgactggta
     7921 gcaatcgaga ccccatttat taaaatcagt gtcacattta ttccacaagt gatcatgggc
     7981 attctttttg gtcctttttg gtcagggata ggcgcagttt tagctgattt agtaggaatg
     8041 gccctcttct cgaaatctgc attctttatt ggatttacat tgaatgcatt tattgaagga
     8101 gcaatctacg gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc cattctggct
     8161 acgcttagtg tcacgctgat catcaactta tttttgacac cattatggct tgcgttgatg
     8221 taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata
     8281 tggttaccga ttcaatcaat tgccatttat tacgtaggac gtagtattcc ttacaaaaaa
     8341 attttaagaa gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc catcccagaa
     8401 gactgcttgt ttttattgtg ctgtgaattg tgcttgatag agccgtcggt aataaccatc
     8461 tttttgaagc agctcatcat gggtgcccat ttcgacaata gctccctgat ccatcactaa
     8521 tatctggtcc gcatttttga ttgtggacag gcgatgggca atcacaaaac tggtttttcc
     8581 ttccatcatt gctaagaagg cttgttggat ttttttctct gtcaatgtat cgactgagct
     8641 ggtagcttca tctaagatca acataggggg attactgatc atcgttcgag caatcgtcaa
     8701 cagttggcgt tgtccatctg atatctttaa cccttgaccg ccaatcttag tatctaattt
     8761 ttccggcaaa cgttctacaa attcatacat ataagaggct tttagggcat cattgatttc
     8821 tgcgtcactt gcttccggat tcccaaaacg aagattttct cgcaatgtac tgtcaaatag
     8881 ccaagtatct tgtaacacca ttccaaaact tttacgtaat gtatcacgtt ggatagagcg
     8941 gatgtcatgg tcatcgactg taattgttcc ttttgttgtg tcataaaaac gcatcagcaa
     9001 attaacaagt gtggattttc cggcccctgt ttttcctact atagcaatgg tttgacctgg
     9061 ataagcagta aaattgaaat cttcgatcaa acgctgcttg gggtcataag aaaaagaaac
     9121 atgatcaaaa gagacttttc ctttgatatt tttgggttcc aaggcatagg catctggcaa
     9181 atcaggcttt tcttctggct gatcgatcaa atcaaatgct cgttctagac cagaaaaagc
     9241 ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat ggtttagaaa actgacttga
     9301 gtagatcgta aagctggaaa tcacaccaac agtgattgac gaaccactct tcaatgccaa
     9361 taggccgcca actaaaccaa tcgataaata agcaaggtga tctacaaaac gagaagacgg
     9421 gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt tcatatagat cagcatttag
     9481 cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa gctttcacga ttttctggtt
     9541 accgattcgt tcggtcacaa agcccgagat atccccaata atcctttgct gtctatcgaa
     9601 atttgtttgt gaagcacgag caacgatcca actcacaagg aaaataattg gtgtagaaca
     9661 aagaacgacc aatgtcagaa gtggacttag acgcatcata aaaataaacg aaagaatgat
     9721 gattgccact ccagaaaata attgattgaa ggccgcagaa attgcaatgg aaatattgtc
     9781 catatcattt gtaaatcgac tgacgatatt tccgtgggag ttttgatcat aataacgaag
     9841 tggcaatcga ttcagatgag caaatgcatc ttttcttaat tttgcaacgg ataagtaagc
     9901 taaccgattg cttaacactt ggatgagcca ttgactgact acgttgatta aaacgatacc
     9961 accgaataga ataagtaaat ggaacagtcg attgaagcgg acctgtcctt ctccaatcat
    10021 ttgatcgata ctttgaccaa tttgtaacgt catcacaact gttgcaatcc cattgatgat
    10081 tcctagtatc acagctagcc aaatgtcttt tgcataggca gtaaggtaag gaatgaatcg
    10141 gcggagcgtc tgcaaggata atttttttac tttcataact attcttcctc ctgtgatgcg
    10201 acgatttcct gatattctgc tgattgagca agtaattctt catgggtacc ctgagcagca
    10261 atcttacctt gatccatcac taagatatga tgtgcttctt gaatagagcg gacacgttgg
    10321 gagataataa tcactgtact gccaagataa gtctgtagcg ccttacgcaa atccaagtct
    10381 gtttgataat ccaacgcact taaagagtca tctaaaatca gtaatgctgg cttggcaatc
    10441 aaagctcttg caatcgtcag tcgctgtttt tgtccgccag agaaattttt cccattttcc
    10501 atgacttttg tatctaaacc atttggaagc tgacggacaa attcttctgc ctgagcgatt
    10561 ttcagagctt cccaacattc tttttctgtg gcgtctttct taccccattg aaggttttca
    10621 cgaatagtcc cggtaaatag tacagaagtt tggggaacaa gagcaatttg ctgacggaga
    10681 tgatccagct gccaagaacg aacatccatt ccgtccatta taactgtacc ggaactgaca
    10741 tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc cgcttccggt tggaccaatg
    10801 attccaagga ctgtatttcg cttcagctga aatgtaatat tttggagaga aagaccagaa
    10861 tcaggagtat aacggaagtc aacatggtca aaagcgataa ttgcttcaga agaaaaatcg
    10921 ggagtagtac tgacctcagc ttcaagaatc gaattttctg tgtctagcac ctctttcacc
    10981 cgatttcctg aagcttcggc acgtgtgaag atcacgacga ggtttgaaac aacgatcagt
    11041 gccaaaagca tttgattcat ataattgatc aaagcaagaa cttgtccttg ttgcaagcta
    11101 ccaatattta ctttgatccc gccaactgtc agcaaacaga tgatcccgac attcatgatc
    11161 aaggttgtag caggtgataa tagggcagag atattggaaa cacgtaaata attgttcgct
    11221 agatcatttg tgcttttatc gaacttctct gtttcttttt ttgtctgcgc aaatgcgcgg
    11281 atgacacgaa caccgctaag attctgacta acgtgttcat tgagctgatc tagtttttct
    11341 tgcacttttt ggtagagagg aacggttttt ttgatgataa aaaataaaat gattgagaaa
    11401 ataggcaata atgctaagaa aaacagacct acttcccagt cgatcacaaa agccatgacc
    11461 actgacccga tacttaaaaa cggtgctctg atcaataaac gaatgaccat tgctaaagct
    11521 agctgaagct gattgatgtc gtttgtcatt cgggtgatca aggtatctgt tccaaaatga
    11581 ttcaactcgt tgtgagagaa agtattgatt tttttgatta gctgattacg taactctgta
    11641 ccaaatcctt gtgaagctac tgaagcatag tattggcaaa tgatagcaca aatcagacca
    11701 atcacagaca tagcgaccat ccataagccc atttcaatga tttttcctcg gtcattcatt
    11761 tttagtccat tatcgatcaa ggaagccatc attaaaggca ggacaagttc gaagacagct
    11821 tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac gatagtcttt tgcataacgt
    11881 aataactgaa tcatatgatt cctcctatta tttatttgtc cacaattgaa aaaacaagta
    11941 ctaccacatt gtagcttagg aaatttagaa tgaaaagaga acaagccttt tcataaatga
    12001 aagttttatt atgaagaagc tttagaaatt gaatagacta ttttttttaa aaaaaagaaa
    12061 atgagaaa
//
LOCUS       DAHUVV010000083        11801 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.086, whole genome shotgun sequence.
ACCESSION   DAHUVV010000083 DAHUVV010000000
VERSION     DAHUVV010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.086"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            306..575
                     /gene="rpsN"
                     /locus_tag="NK767_002175"
     CDS             306..575
                     /gene="rpsN"
                     /locus_tag="NK767_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCH6695157.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            590..769
                     /gene="rpmF"
                     /locus_tag="NK767_002176"
     CDS             590..769
                     /gene="rpmF"
                     /locus_tag="NK767_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCH6695158.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            801..1178
                     /locus_tag="NK767_002177"
     CDS             801..1178
                     /locus_tag="NK767_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695159.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1199..1348
                     /gene="rpmG"
                     /locus_tag="NK767_002178"
     CDS             1199..1348
                     /gene="rpmG"
                     /locus_tag="NK767_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCH6695160.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1372..2355
                     /locus_tag="NK767_002179"
     CDS             1372..2355
                     /locus_tag="NK767_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCH6695161.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2385..2507
                     /locus_tag="NK767_002180"
     CDS             2385..2507
                     /locus_tag="NK767_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCH6695162.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2525..4048
                     /locus_tag="NK767_002181"
     CDS             2525..4048
                     /locus_tag="NK767_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCH6695163.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4072..4311
                     /locus_tag="NK767_002182"
     CDS             4072..4311
                     /locus_tag="NK767_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6695164.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4504..4965)
                     /locus_tag="NK767_002183"
     CDS             complement(4504..4965)
                     /locus_tag="NK767_002183"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCH6695165.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5160..5378)
                     /locus_tag="NK767_002184"
     CDS             complement(5160..5378)
                     /locus_tag="NK767_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6695166.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5496..7112
                     /locus_tag="NK767_002185"
     CDS             5496..7112
                     /locus_tag="NK767_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCH6695167.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7353..8693)
                     /locus_tag="NK767_002186"
                     /pseudo
     CDS             complement(7353..8693)
                     /locus_tag="NK767_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8969..9124)
                     /locus_tag="NK767_002187"
     CDS             complement(8969..9124)
                     /locus_tag="NK767_002187"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCH6695168.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9420..9752)
                     /locus_tag="NK767_002188"
     CDS             complement(9420..9752)
                     /locus_tag="NK767_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6695169.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10266..10457)
                     /locus_tag="NK767_002189"
     CDS             complement(10266..10457)
                     /locus_tag="NK767_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6695170.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10551..10937)
                     /locus_tag="NK767_002190"
     CDS             complement(10551..10937)
                     /locus_tag="NK767_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCH6695171.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            11631..>11801
                     /locus_tag="NK767_002191"
     CDS             11631..>11801
                     /locus_tag="NK767_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HCH6695172.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTK"
BASE COUNT         3937 a         1834 c         2254 g         3776 t
ORIGIN      
        1 ttatagtttc tttcatattg ccgttttaag tagaggaaaa gctacttaaa acggtatttg
       61 tgtttgattg gactaataaa agtgaattct tatttttcag agcctaaact gcatattctt
      121 ttaaatgatt tgattatctc tatgtaccga taaattcgtt ttcaaatagt tatctattca
      181 tatgagtttt atttcatgtt atttattgtt gacaagagca actcttgttg gtaaagtatc
      241 agtgtcaaaa cgtaacgatt acgatttgag gtgtctaaat caaaataaaa ggagtataaa
      301 ggtatatggc aaaaaagtcg aaaattgcta aagcaaagaa acaaatggcg atgattgaaa
      361 agtatgcaga taaacgtcag gaattaaaag ccgcaggaga tcgcaccgct ctcgctaaat
      421 tgccaagaga ttcaaatccg aatcgtctga ggcttcgtga tcagacggat ggtcgtccaa
      481 gagggtatat gcgaaagttc ggtatgtccc gtattaagtt tagagaactt gcccatcaag
      541 ggttgattcc tggagtaaaa aaagcaagtt ggtaaaagga ggaaatatca tggcagtacc
      601 agcaagaaaa acatctaagg cgaagaaacg gttaagaaga actcatcaaa ctgtagtaaa
      661 accagaaatt tcatttgacg aagcgaatgg tgattataga cgtagccatc atgtatcttt
      721 aaaagggtac tataatggaa agaaagtaat taaaggagct tcaaaatagt ttaatatata
      781 acttttagga gggaatcaaa atggaaatta tttacccacc tttagttgaa gaaggattaa
      841 agtattatct tgaaacgacc cagcaatcgc tagataaatg cactttttat cgttcgatgg
      901 tagagagagg aattattact gaaacgggtt taccgaccca acaggcaatt gaaaatggat
      961 tagtcaaaga ttactatgag gaccaaggct tatcatttga tgatttttta agaatctatc
     1021 caatttttga agagtatgac gaggagttat ttcaatgtat cgatggttat tgggagatac
     1081 ctgttgatat gaaagaaaat ttagtttcac aattggaatc tggcgagtta acttttgaag
     1141 atgcacaaca aattcaagca tatcttgaag atcgataagt attgaaggag gaagatttat
     1201 gcgtcaaaat attattttgg aatgtgtcga aactggagaa cgtctgtatc tcaccagcaa
     1261 aaataaacgg aataaccctg aacggttaga attaaaaaag tattcgccaa aattgcgcag
     1321 acgggcaatt tttaaagaag tgaaatagct aagtggtaaa aaggtgacaa aatgggcata
     1381 ccagttacaa tcgtttctgg gtttttaggg gctggaaaaa caacgctaat caatcaagcc
     1441 ttgcaagtct caccctaccc cagggaagaa attattataa ttgaaaatga gtttggtgaa
     1501 gttgggattg atcacaaatt attagttcat agcgaggaac gggtgttaca actaaataat
     1561 ggatgtatgt gttgcagtct tcgcagtgat ttattagcaa tattgatttc tttactggaa
     1621 acaatagaag aacatcaaca accaatttca caaatcatca ttgaaactac aggtatagca
     1681 gatccccaac caatcattca aacactttta acagcgcctc aactaagagg aagagtatat
     1741 atcgatagtt tattgactgt tattgatagt gacagttttg aacttattga aagagaacca
     1801 caagccctta agcaacttgt catggcagat cgcatttttg tgtctagaaa acaaaacttg
     1861 aatttatctg cactaaaaaa attacagaga gaaatcaaaa gtattaaccc tttatccgat
     1921 attcgaatgt tcacacatag tgaaacgatt caaaaaaagg atttttataa tttgaaaaaa
     1981 tttaatcatc ccttagtttt tgaaagtgat tcatcagaaa atcactataa tggcaaatac
     2041 tatcatcaac accatcaagt acatgagttt aaatcaattc tcttaacttc agatcaagac
     2101 ataaaaaaag attttttact ccaatggatt gactggctga ttttaaccaa ccaagggagc
     2161 ctgtatcgtt ttaaaggatt gattgcttta caagagcagg attttatagt tgccatgcaa
     2221 gaggtaaacg aacaggttaa ttttcaatac accactcgac aaaaagacca aaccactatt
     2281 attctaatcg gaaaaatgtt ggatgaagaa aggatcaaac agagttttga taaattactg
     2341 gcggaaagca aataatgata agtagtgatt gggggtaatt tctaatggag aaaacggata
     2401 tatctagtgc atatcgacga cttaaaagcc cgaatattaa aactagaaaa agggcattaa
     2461 aaattatcaa agaacataaa cgaaataaaa tgaagaaatt agcataatta aggaaggaaa
     2521 aagaatgaag aaaatcagtg taggacttat tgggatagca gctctaggat tattgggagc
     2581 ttgctccagc actaatgatg caaaagtatc gaatgataaa gatggaaaat tagaaattgt
     2641 aacaacattc tatccaatgt atgatttcac caaaaacatc gtcggcgatg aagcgaacgt
     2701 ggatttaatg gttccagcgg gatcagaacc tcatgactat gagccctcag ctaaggatat
     2761 ggcaaaagct catgatgctg atgtttttgt atatcataac gaaaacatgg aatcatgggt
     2821 accaaaagct aaagaaagtt ggaagaaagc tgggcctaat gtagtagaag gaacaaaaga
     2881 catgatttta ttgccaggta gtgaagaaga agatcatgat cacggagaag aagatcacca
     2941 tcatgaacta gatcctcata cgtgggtttc acccaaaatg gcaattaagg aagtttcaaa
     3001 tatcaaggat cagttagtta aattgtatcc gaaaaaagca aaagtatttg agacaaatgc
     3061 tgaaaagtat ttgactaaac tgaaacggtt ggatgctgac tataccacta gtcttaaaga
     3121 agcgaaacaa aaaagttttg tgacgcaaca cgcagccttt ggatatttag ctttggatta
     3181 tggattgaaa caagttccta ttgctggact aagtcctgaa gaagagccat cttctggtcg
     3241 attagctgaa ttaaaagagt atgttaaaaa aaataagatc aattatattt actttgaaaa
     3301 gaatgctaat gataaaattg caaaaacttt ggccaacgaa gctgggataa aattggaagt
     3361 actgaatcct cttgaaagtc ttacaaaaga acaaatggat aatggtgaag attatgtttc
     3421 agtaatggaa gacaatctaa aagcgttaga aaaaacaact atggttgctg gtgaagaggt
     3481 agttccagaa aaagaagcga aggatgaaaa gacagttgct aatgggtact ttaaagatgc
     3541 cgatgtgaag gatcgcgaat tgagtgatta tacaggcgaa tggcaatctg tttatccgtt
     3601 gttaaaagat ggaattttgg atgaagtttt cgattacaaa gcaaaaatca ataaagacat
     3661 gacagctgcc gagtacaaag attattatac tactggctat aagacagata ttgatactat
     3721 caacattaaa gacaacacca ttgattttgt agtcaacggt gaacatcatc aatacacata
     3781 caaatatgtt ggatacaaaa ttttgaatta tgaaaaagga aaccgtggtg ttcgttttaa
     3841 ttttgaaact gatgatgcag gtgctggtcg ttttaaatat attcaattta gtgatcatgg
     3901 aattgcacca agtaaggcag agcatttcca tattttcttt ggtggagaaa gtcaagaaaa
     3961 actctataat gaattgcata attggcctac tttctatcca gcttcattgt ctaaacatga
     4021 aattgctcaa gaaatgatgg ctcattaaaa ttcaattttt ggaggaaata catggtaaga
     4081 aaagtattat tgtctatgga ttccaagaat attgatgctt atcggcgtta tcgatttgca
     4141 aagcaaatga gattaaatgg gagtacatta gctgatattg ctgcaacttt acatgttagt
     4201 attgacacag ttcatagagt attaaaacgg gatcctgatt gttgttttat caaggtcaat
     4261 actggaagaa gtagacgttt agcaaaacgg cataatagct tggccaatta attgataata
     4321 ccagagtaga tcatttttcc cttatttgga tttgatctgc tttttattat gtcttgaatt
     4381 gtcaaatcaa gcgcaacact ttatcaaaga agacttcata aggtgttctc caatctaaac
     4441 actttctagg tctgttattc agtttttcaa tcatctgatt tatcgttgag ttatctaccg
     4501 tagctatctc tttcgatttt ggcaagtatt ctcgaatgag tctattcgta ttttaatttg
     4561 ttcattgttg ccaagggcat gaggatctgg aaaataaaag ggcatattat tcaatgcttc
     4621 tgtaactgcg aaatgattgg agaattcttt tcctctatct ggagtgatcg ttttgaccgc
     4681 ctcatttgga atactgctta aaagttcaat cattttctcg ctcacataag cggctttctt
     4741 tctggggacc ttctttcctg ctaaaaggaa gcatgatttt ctgtctgtca aagtcactaa
     4801 acaagtacca ccagttttgc cagctacaat atcggctttc caatgaccaa aatcacttct
     4861 atcatctgcg gattttggac ggccatggat cgaatgagta aggcaaatct ttccacgaga
     4921 ctcgacatga ttttttgtat gtcttgtttt tcctcgatgg tgcaatgaac ggatcaggcc
     4981 tttattgccg tgtgagaggg gttctgtatc gaaaagatca ttgcaaattg cacgttagat
     5041 cgtgataaaa ctaattgaaa agtccatatg ttcatgcttc aaacgattcg agatttcctc
     5101 tggcgaccac tgaaaatcga caaataaatg acgaacaatt gaccaaactc acggatcatt
     5161 tagaatgtga taacgtccgc atttttttct tcgataagca taattttttt ccgctttgga
     5221 aggtgaatag tttttttctt gtttcttctc agttcatgta aaatagtaaa cgggctatgc
     5281 tgaatgtcgt tgccaattat actaatagaa aatccttctt cgtgaagcag aaagattctt
     5341 tctcgttcga gtatagtaag atgacgatac tggctcataa ttattttcct tctaggagtg
     5401 ttctcgacaa acttatacta gttggatatc gattatgact catttttatg tgttgcactt
     5461 aaagtgtaaa ttcaaggtat agaaagaggt tccctatgaa aaaaatacct attacaatct
     5521 taactggcta tcttggttca ggaaaaacga tacttctaaa tcatattctg tacggtgatc
     5581 acggaaagga aattgctgtc attgtaaatg acatagggga agtaaatgtt gatggggtat
     5641 tagtcgacaa gaaaggtttt aaaagaactg gagaaaaact tgtttcgatg ccgaatggat
     5701 gcatttgttg tacattacgc gaagatttga ttgttgaact agatcaatta gtgcaatttt
     5761 ctgacttgaa tcaaattgtt attgaagctt caggaatcag caaaccaatt ccaattattc
     5821 aagcaattat attagctaaa aaccctttag acattgattt gacccaacga ttagagattg
     5881 gtgccattat tatagtagtg gatgtaaaac gtttttggga taatttcgaa acgggtgaaa
     5941 agattcagga aagaaaagct gaaggtaatg tagttgaaga agaacaggat attcgtggct
     6001 tgttaattga tcaattcgag tgttgtaaca tattactttt aaataatagt gacttagtgt
     6061 caaccaagac gatttacaaa attaacggtc taattgctaa gttacagtcc aatgccaaaa
     6121 taattaggac aattaaaggt gaagtagact tatcgaaaat tctaaataca aatcttttta
     6181 atatagatga gacttcctat tcagtgggct ggttcaaaga gttagagctt ggctatcaaa
     6241 atcatattcc tgaaacagaa gaatatggta tctcaagttt tatctatcga aatcggttac
     6301 tccttgacgc acaaaaattt gcccatctta cggataaaaa ttttcctggc aatattacaa
     6361 gagcaaaagg gtttctttgg tttgcccaac atagtgattt ttcgacgatc tttgaaacat
     6421 cgggtagcta taaaagtatt gaatcattaa attattgggt agctagttta cctaaaattg
     6481 agtgtcaaat gattatacaa aaaaatcccg aattgaagga agcctggcat ccgttgtttg
     6541 gtgaacgcat taataaacta gtttttattg gtatgaagat ggatcaacaa tgtattaagg
     6601 aacaacttga gggttgttta actgattctc ttaatgcaga taatttccga aaaatttcac
     6661 catttccatg gaaaaagtct aatatgttta gccagatatc gttaatgaaa tttttaagaa
     6721 aagctggtgt caagataggc gatgtcataa ttattggcat tttgattatt acctctttta
     6781 ttcccttatt tgtattctct atagaaaagg agaatgaaac aactgaagat gctatcaaat
     6841 atgccatcgt tcgaattgat ggcaaggaaa tggatcgctt cgatttggat aagatcaatc
     6901 ataaactcgt gaattatcat cctgcgaagg gacaatataa tattgttgag attaaagatg
     6961 gaagaattcg agttaaagaa gataatagtc ctgatcaaat cgcggttaga actggatgga
     7021 tctcaaaacc cggtcaaacg agtatttgtt taccccataa gttagttatt agtattgaga
     7081 aaaaagaaag cacagattac tacatttact aatctggaaa tagatgagga ggtttttaat
     7141 aagaaaaaga tattattatt aataatagca ggggctttat taagtgtgtt aagcgcttgt
     7201 ggacaagaaa aagaagcggt gacaacaggc acaagtatct ttaacgggac ctgcaatggt
     7261 ttagttaaca gtcggttaga ttacttattt tggctcttcg tcaatgagtg ttggtgaaga
     7321 aattcaatag aataatcgag atactcacac ggctagacag ctatcttgtc tagccgtgtt
     7381 gttgttctgg ctttagtcag ttgggtagta ttcttagttt tcttaaaata ttttcctcga
     7441 tataaaaaga ttcgttctcg gtagttttga aagtttctat agccataggc aattcgtttt
     7501 agtactttga tatggttatt caaacattct atagggccgt tggaataggg aacgcgcaac
     7561 gctcgtttga tttctgtttg gtatttcttg aaggtcgtga acactggttg gtagtgctct
     7621 ggcaactcct tgtagtcttc ttttaggagc gcgacgaatt caggaacatc ttgtcttctg
     7681 ataacggaaa gaaaatcctg gtagacttcg tagcctcgct ttaaggaatc atcataagat
     7741 aatagccgat cgactatctc tgcttctgtc agatgatctc gaaatgacgg acgccagtag
     7801 cgatgctgac ctttcaattc ccacgcattc ttttgaagta gtttccagta tttctttaag
     7861 tgcttcgacc gacgatcccc ttttttaaag gtcttcatta cttgaattcg gtggttggta
     7921 aaagcccgac caatgtgttg gctaatatga aagcgatcgg cgacgattcg ggcattcgga
     7981 aaacactctt tcaccaatgt gcgatagggt gtatagaaat ctgaaaccac tagacgcact
     8041 tgttcacgga cttttatagg gtagcgcaag aaatagtcgc gtaaaggatt gagttgccga
     8101 ttttccacaa tatcaatcag ttgatgggtc ttgccgtcca tcatgacaaa gctcatggct
     8161 cccgagacat ttttcactga tttgaattca tcgaaacaca ccacttctgg taacagctgg
     8221 ctcaaagaaa tggttttagg ccgaaggctg cgaagtattc gaatgacagt ggtcggagaa
     8281 atgtgcttca tacgtgcgat caaagacata gaaagaggtt cgcttaatag ctcaagaatg
     8341 gcttgtttga ctctgcgagc aatggagcag cgacgatcac taactgaatc ttctgctaaa
     8401 aaagtctttc cacagtgttt acatttgaag cgctgtttgt taatccgcag aatggtcttg
     8461 tagttactga catcattcaa taagatggat acttttttcc agccctattt aatgatcgtt
     8521 tgttcatttt gatagtggcg atgaaagcat tcctccggtt gataggtcaa tgtgccatca
     8581 aaaacaaaac atgtttcgtc attgattcgc tctttgcgaa aggcatcttc atgaaagata
     8641 agatttaggt ctagaatatc tagtaactct ttggtaaact ctgaatagga catcttgaaa
     8701 cactcctttg aatggttttc gtcgacttta ttctaaagga ttttaaggtg tctgtttgcg
     8761 tttattagaa aaaagaaaaa gtattggcaa cagaaggcgt caccaacact agatattata
     8821 gatcccttat tttcttcgct tcaaattgtc aaggagtcat cataaatttt gtatggattc
     8881 ttttgtttgg gtagtagtct tctaaataat cttggattaa caccttcatc tctattttac
     8941 ttttatattt gttggagtcg attaattctc atttgattga tcgatgaaac gcttcaatta
     9001 ctgcattgtc aacaggagtc ccagtttggc tcactgaatg tgtgatgtgg tggtcgttca
     9061 acagctcttg ataaataaaa gaccgatgct ggctacctct atcggaatga attaccgtag
     9121 gcaattctcc ttaataaatt tgtaatggac ggacgacaat ctcttttttc atgtgtgaat
     9181 ctaccatata gtaacgtaga atacgagttg ctaagtcaat ataggtggac aaatagaggc
     9241 gaccgtcatt acatggaatg taagtaatat ccatcaccca aacagcatca atttggttct
     9301 gttcaaatta tcgattcaac atttttttac catttctttg attgtttttt ctctaccatg
     9361 agaagatttg cgtcgatgaa atcctcttgt gcataaattc atttcacgca taagaacagc
     9421 tacaacacgt ttattgattt cgattccttc atcgtaaagt ttttgagtga tccctggaga
     9481 tccgtatcga tctttgtgtt cgtagaacac tcgtcttcct cttttagtcg tttattttcc
     9541 tgttcaagaa tgtagaaatc tgctttatca ggtaatcttt ttccttgtcc agcaaaagca
     9601 tttttcccgt atcgacgata ttctgaaacc catcgggaaa ttgttggacg agagatattg
     9661 tattcttcag caattttctt ggtagttact tttttctcaa ggatatcctg tgaaatcttt
     9721 agtttaaatt tacgatcata tgtttttctc aatagagttc attccttccg gtaacgtaac
     9781 tgatagtcaa gaaaagtgta tcatagccca acttagtatc agggtttgtc aactaaactg
     9841 tggaagttga tagataagag tttttaacaa ccaaaattct cttgactatt tttgaaatca
     9901 gacaaatttt gattggacac atagttgaat aaatctaccg tcgttacgga aggtaaatcg
     9961 catgcttttc aattctagag gagaagtcct cgttgaagta ccatagccca gttctggatt
    10021 cgacctcctg cccacttcat acgtaaataa agtaatttta ggagtgagac tgtgacaaaa
    10081 gttattttta agctaaaaac gaattaaaaa acgttgataa ttaaaatcat tcgttttaga
    10141 ttctatgaat cattatagaa cgctgtaaaa tcgatacttt ataattataa agaagatagt
    10201 ttcaaattta ttgttcgaaa tttgaaacta tctttttact tatgtcacag actcccttag
    10261 ttgagttatt ctttaatttc gaatttcgag atagctagat atattgcaag catacattct
    10321 tgataacaat cttgatcata ttttccagtt ctccaggatt gcttatgaat caaagagcta
    10381 aatcttttga gaattgcctc aacagcttct tcatttccag cctttgctaa aatgaaatct
    10441 tcttttaaag aattcataaa tctaccttct tcccatggac ttataatcta tttctttggg
    10501 aaggaggaga acgagcaaag aagcatacaa aattagttat tatgcagata ttaaaccagc
    10561 attttttgaa tttttttgta aagtcgatgt tttaaatttg tcgcaccctg tcttgtaatc
    10621 cccagaacct ttccaatttc tttatcagtt ttatcaaaga taaatttttc gataagaaaa
    10681 atttgttcag gttctttcaa caaaggaagg atttttgcta attttatatc ttcaacaagg
    10741 agagtcacat ttaaaacatt aatcggttgt ttaaatttta tagtatcttt agtaaacaaa
    10801 tatggtggga gttgatcaag ttgttcatgt ttttgaattt cttcttgttt tttataataa
    10861 gtattagcgg tgttcaaaat gaccttttca aagaatcgaa tcaactgggc atttacgcgt
    10921 tcgtcgttat ctgacatatt gcacacctcc tccattaaga tttaaaggga aaagaggcaa
    10981 aaaacacgaa aagaagctaa tgcaattatg ctgcgctgcg aataacttct ataagctcac
    11041 atgcattgat aggactgctg atgtttattt tcaaaaatgt ttttttagtt gataatggat
    11101 agttatcttt atacttagaa aagaaaattc atatatcttc ttaaatatca aacatgtcct
    11161 tatggctaag aaaatgtctt gagcttttta cgtataataa atcttttcta aaacctcctt
    11221 ttggataata ttcttattcg tgtatagatt gtatttttta tttcttcccc cttcataatt
    11281 aacaactaga aaagagttcg aatagacacg tatttgaacg aaaattttta ttggattccc
    11341 aaattggaat atttagaaac gaaaaattcc tattatctgt atagtgctta gataataacg
    11401 gatcataata attattgtct ttgaactaga tatatgtaaa tagtattcat catgatgaaa
    11461 aatgatgagt tctcggtgat aaaatgtact ttttacggtg gaatatacat gaaagcgata
    11521 agtggatatt ttataatgaa gtaagttagc ttattttgac agaggaatag tattttagat
    11581 aacgtataaa atactattta atgatgtaga aaggaaagag agaaacaaaa gtgttttcag
    11641 ataaagaagt cttattaaag tctcttcatg tttgtttaaa gttacctatc gtggttttta
    11701 attccgaatt cagtattata aaagagtatc gctcagatag aactacttat tttttttatg
    11761 attataaaaa gttactaact gataatatta aaaaaactaa a
//
LOCUS       DAHUVV010000084        11786 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.087, whole genome shotgun sequence.
ACCESSION   DAHUVV010000084 DAHUVV010000000
VERSION     DAHUVV010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.087"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(284..2068)
                     /locus_tag="NK767_002192"
     CDS             complement(284..2068)
                     /locus_tag="NK767_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6695173.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(2068..3819)
                     /locus_tag="NK767_002193"
     CDS             complement(2068..3819)
                     /locus_tag="NK767_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6695174.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(3961..4185)
                     /locus_tag="NK767_002194"
     CDS             complement(3961..4185)
                     /locus_tag="NK767_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCH6695175.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            4436..6022
                     /locus_tag="NK767_002195"
     CDS             4436..6022
                     /locus_tag="NK767_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCH6695176.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(6088..7863)
                     /locus_tag="NK767_002196"
     CDS             complement(6088..7863)
                     /locus_tag="NK767_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HCH6695177.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8074..8976)
                     /locus_tag="NK767_002197"
     CDS             complement(8074..8976)
                     /locus_tag="NK767_002197"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCH6695178.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            9255..11600
                     /locus_tag="NK767_002198"
     CDS             9255..11600
                     /locus_tag="NK767_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCH6695179.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT         3585 a         2408 c         2195 g         3598 t
ORIGIN      
        1 acccaaaaag ccgtccatgg aaccatggga gactcatcag gtttagcttg catcaggcaa
       61 tcactatcca agttgtatcg ttttctttat attatcgaaa gcaaacgctt ttgtcaacgc
      121 tttcttagtg gttattagcc gcttttgact agctgtcgtc gtctttttta aatcgggtga
      181 ctacgccact aacgctcaca gatgaagaat ctgcgaagct ctgttttatt ttcactctag
      241 cgcttagggc aaaatgctac tatgctactg tcacaacctc tttttacaca ccactgcttt
      301 gcagtttgta catatctgca tacaaaccat tttgcgctag aagtgtttca tgattcccac
      361 gctctactat atgacctttg tctaaaacta aaatcaattc tgcatcccga atcgtggaca
      421 gccgatgggc aatcgctatc gttgtccgtc cttgtcgcat ttttgccaat ccttcttgga
      481 tcaatccttc ggtttcagta tcgatatttg ctgttgcttc atccaatact agtattttag
      541 gatctgtcac gattgttcgc gcaaaagtga tcagctgtcg ttgtccacta gaatagctgg
      601 caccgtgttc gatcactttt gagtgatatc cttttggtaa ttcatcgatg aacttgtttg
      661 cttgaacaaa ctcagctgca tggcgaatct gttcgtctgt gatagctgga ttcatcatcc
      721 ggatatttcc tgcaatatct ccataaaaca agaaggcatc ttgcaagacc aatcccattt
      781 tcttacgtaa ctcttccatt ggatattctc gaatgtcatg atcatcaatc aagatttggc
      841 cttcataaaa ttcgtaaaac cgcatcaata cgttgatgat cgagcttttt ccgcttccgg
      901 tgtgccccac taatgcaacg gtctcacccg gattagcgac aaaagagata ttgttcaata
      961 cattgtgctc tccatcataa gaaaagctta cattgcgaaa ctcgatctta cctgaagtaa
     1021 tcgcttgatc tgctccagga ttttgttgcg gtgcatattc ttctgtatcc atgattttca
     1081 aaatacgact accggcaacc atcccatctg taaaggtgct caaaaagtcc atcatttgag
     1141 acattggatt aaaaaattcc tggacatagg tcgtaaatgc atagatcagt ccaacttcta
     1201 cgggagaatg caacgcatcg atcccaaaca aagtcaacgc caacgcaatc gccaatgcat
     1261 agagaaaatt gatgattgga cttaacagaa tcgaattcgt tttgatcatc gcatagcgcg
     1321 tgtctaaata ctcgttgttc acttgttcaa attctttagc taaacgagct tcttggcgaa
     1381 actgctggat aatacgcatg ccggaaatcg attcattcaa tttcgtgttc agctgactta
     1441 atttttcccg catatttcgg taaatacgag aactgaattt ctggtaatac cagatgacga
     1501 ttcctaaaat cggcaagaac accaaagtcc acaaggcaat ttctccattg atttgaaaca
     1561 tggcaacgaa agaagaaacg actgcaaaga tcccagtcaa taccatcaaa aagacatacc
     1621 aaaattcaaa tagcgtttct gtgtcattcg tcactcgaga aacggtcgat cctgccggtg
     1681 tctggtcaaa ataacgcata cctagtgtat gcagcttttc aaataatctt acccgaacat
     1741 actgataggt cttcaatgaa gccatggaat acaaatacca ttggaaaaac caaatgatac
     1801 ttttgactat gactccaaac aagtacacac cagcaaaaaa caaaataact tgtgtcgtag
     1861 ctgactgttt agccagatga tcatccataa aagtttggat gatccttggc aataggacat
     1921 tgatcacaga cagggcaaac gcaaataaga tagcaacgat gaacgttctt ctaaaaggtt
     1981 tggcgaactt catcagtcgt ttgatgatct tcttctgttc tttaagtgga atcgattttg
     2041 tccattccga ttgataattt tcatccatca ggcctcgcct ccttctatct tcgcttctaa
     2101 ttgctgtttt tcccacattc gttcatacca gccacctaat gcaagcagct cgtggtgggt
     2161 tcctcgttca atgattttcc cttgatcgac aacaatgatt tcttttgcat gcatcacgct
     2221 gcttaaacgg tgagctgcaa tgatggttgt tttctctttt cttatttctt ttaaattatg
     2281 caagatagct tcttctgttt tggcatctac tgcagaaagt gcatcatcta agatcagcaa
     2341 ttcaggattt ttgatcaatg ctcgagcaat cgaaatcctc tgtttctgtc cgccagataa
     2401 agagactcca cgttctccaa ctaaggtatc ataacctttt ggcaattcct tgatttcatt
     2461 gttgataaat gctaaagaag ccgcatgctc tacttcttct tctgaaagtt ttggattaga
     2521 aaaacggata ttatctcgaa tggtcatcga aaacaggaag tgatcctgag gcacataacc
     2581 gattgatcct aataaagcat tcaatgaata ttctttgatc gaacgtccgc caaattggat
     2641 tttcccttga taatcatcgt attcacgcat cagtaatttg atgattgtcg tttttcccgc
     2701 accagttttc ccaacaatac ctaatgtttc tccttcttgg atcgcaaatt tgatttgttc
     2761 taatgctggt gattcttctt tcggatagtt aaaagaggaa atagctacat tcagctcgcc
     2821 ttttgccggg gtctgcactg cacccagttg ttccacgata tgcgtctttt catgcaaaag
     2881 ttcattgacg cgatcataag aagcatttcc tctttccaat acgttgaaca atcgaccaat
     2941 cgcaaacatc ggccaaacaa gcataccgat atagctaata aatgagacca tctgcccgat
     3001 tgagatcgtg ccttccaaga catactttcc acctaaaata attgttgcaa cataagacag
     3061 accgataatc aaggtaatga atggatcaaa taaggagtct aaaaaattga ctcgtttgtt
     3121 tttaataatt gcatcttcaa tcttttcttc aaaatccgcg agatcttctt tttcttgtcc
     3181 aaatgtttta atgactttga tcccagtgat actttcttgt gtcttatcat tgatactaga
     3241 aaaagcttct tgcgaatcac gaaaagcatc gtggagtttt gaccccagca ctcgtgaagt
     3301 aacggctagc aaaggcagag gaatcagcgc aatcagtgtt aagcgccaat caataaacaa
     3361 aatcatcgca atgatcgttg ttccgcccgt aatcactgaa tctgcaaagg tcaagattcc
     3421 agctcctgct acattctgga ttgcattcaa atcattcgtt gcatgagcca tcaaatctcc
     3481 agtccgatat ttttggtaaa agagattgtc catttctgta aaatgtttga atagctgctg
     3541 ccgaagttct ctctccaacc gagctgcact tccccaaata ttcgtccgcc agatataacg
     3601 aaaaagatat tgtaagaaag ctgcaaggat caataataag atccaccagg aaatgatctt
     3661 tattgataaa ttcttatcgg cgatctcatc aatgatgatc ccaatcactt ttggaggaac
     3721 taactgtacc aaggccactg cgaataggga gaaaatccca attaaataat gtttcttttc
     3781 ctgacgaaaa aaccaaccta attttctaaa aatcgacatt ttactcctcc tttgtcttta
     3841 tcaagagcat aggattgtct gtgcttgtct tttttgctga atagtctgaa tagtctaaca
     3901 aattacgaaa taaaaaacgg actgaaaaaa tcagcccgca aaaagtcttt gggtcagaac
     3961 ctattttttc ccttggttct tcatttgctg catcatctgg cgaattttct tctcagatgg
     4021 tttttgcccc attgacatca tcattgatcg caacatgtcc tcattaacgg gaggattttt
     4081 ttctaaatag tctttcatat atttacgcgc taggaaaaag ccacctactg cgccgattag
     4141 caaagcaata atagcaataa gtacgacaat acctgttgat accatataaa tctctccttt
     4201 ctatttccga attgctctca atgatttatt ttactaaagt ttattgaaaa aagaaaggcg
     4261 tattctttag tttcatgcaa aaaatagtgg tttttttaga gaagaacaaa atttctttca
     4321 ttaaccagca agacacacca cgaaaaaaaa ttaggcatac cgaatctttt tctttaattt
     4381 tttctttctg aggtgtagaa tgtaatcgta gataaatagg aaaggtggtt ttttcttgaa
     4441 taatcaatcc aacaaacaat ttcataagca tcgacttatt gaacatacaa atggtttatc
     4501 tttagctgaa gtaaatggaa cagtggatgt accaaaggga aaaagttttt ttaaaacact
     4561 tcttgcttac tctggaccag gtgcattagt agcagttggt tatatggatc caggtaactg
     4621 gtccacttcg atcactggtg gacaaaattt ccaatattta ttgatgtcag ttattttgat
     4681 gtctagtcta attgctatgc ttttacagta tatggcagca aaattaggaa tcgtgaccca
     4741 aatggatttg gcccaagcaa tccgagcacg gacaagtaag acattgggtg tcattttatg
     4801 gatcttgact gaattagcca ttatggcaac agacatcgca gaagtcattg gtgcagcaat
     4861 cgccctctac ttactttttg atattccatt ggtgattgcc gtctttatta ctgtattaga
     4921 tgttttcgtt ctcttactac tgacaaaagt cgggtttcgt aagatcgaag cactggttgt
     4981 ctgcttaatt ttcgtcattt tattcgtttt tgtctatcaa gttggcttgt ctaacccaga
     5041 ttggcacagt ttatttgctg gctttatccc taacagtcat acattctcag actcaccggt
     5101 catcggcggc gaaactccgc tgactggagc tttagggatt attggtgcaa cagtcatgcc
     5161 gcataactta tatcttcatt cagcagtttc acaaactcgg aagatcgatc accaaaacga
     5221 agaagatgta gcacaagctg tacgtttttc tacttgggat tctaatatcc agttgtcaat
     5281 ggcttttcta gtcaatgcac tgctattgat catgggtgtc gctgtcttca aaaccggttc
     5341 tgtccaagat ccttcctttt ttggtctgta tgaagcttta tctgatccat cagctatgag
     5401 caatggtatt ttgattgctg tagcaaaatc tggtatcttg tccactttat ttgctgtagc
     5461 acttcttgct tctggccaga actctactat tactggtacg ttgaccggtc aagtcatcat
     5521 ggaaggattc atccatatgc gtatcccgat ttggctaaga agactggtca cacgattgct
     5581 ttctgtgatt cctgtattga tttgtgtttt gtttacaagt acaaaaggaa cgatcgaaga
     5641 acatgccgct ttgaataact taatgaatga atcccaggtg ttcttggctt ttgcgttgcc
     5701 attttcgatg attcctttac tgatgatgac caacagccaa gcggagatgg gtagccgatt
     5761 taaaaattca ttcatcgtaa aacttcttgg ctggttctct gtgattagtc taacttactt
     5821 gaacttacga ggattaccag gacagatcga aagtttcttc ggcgagcaag ccacttccgc
     5881 tcaactagca ttggctgatc acatcgctta tgtaatcatt gttcttgtcc ttctcctgtt
     5941 ggcatggacg attggtgatt tatataaagg aaacaaaaaa tatgaacagc ttttgcagga
     6001 aacggtagaa gaaatcgaat aaaaactgat ttttccacac aaagaccctt gatagttagg
     6061 ttaaaaatct agctaccaag ggtcttttta tttagaagat aaagcttttt tcagatttaa
     6121 tttactgaaa actagcatgc ttgttactac gatgaattgc gttaatggca cgcctgcacc
     6181 agtgtagaca cttaaaaaat agtccttttt ttctggcgca taagaacaaa accatgagac
     6241 accgaaaaga aaaaccaatt gtgtcagtat taatccggtg accacaatca aagaggaact
     6301 ataataggca cgatcagctt ttgcaaatac ggttcctagt atcataagta atatccctgc
     6361 tattgaagca atcagcacgg gaatagccag aacttggtaa atttttatga tgacattcgc
     6421 gacttttgct gattttgtta attggaaatt aggtgttctc ggttcattac tatcgatcca
     6481 attctccatt agcggaaggt ttaataattc ttgagcctgt ttgacttgtt cttgcgttcc
     6541 gaaactataa tttcctcctt gatggaaatc tttatatcgc catgcataag cgtatcctga
     6601 ttgaaggaaa tcgaatacga ttggcagatc actcatcgtc tttccatctc ctgatcccat
     6661 cagatagaac tcttttttct ttttgatggt tcccttgtga taggcttctt ctaattcttg
     6721 gttgactttt ttccaaaaat cattggtttc ttttgcattg ttttggtaat aaccagattc
     6781 gcttaccaca tctcgcaatg cccaaaagat gatatctccg ggtaattctt ttacttttcc
     6841 accttgagtc cacgcgtgag tcgtatataa ttcgtcaata cgggtagaaa cagtatggaa
     6901 tgtaggagat gcggctaatg ctttatttag tgcagcatgt gagacccata tctcactgtt
     6961 ttctttattc aagttcgttt catcatctat tcggatcagt tgtttcatca aacgtccaaa
     7021 ttctccactg gaacggtcat tgatggttgc aataccgtat gttgtctcat ttttatattc
     7081 gatccactga tagacgatgg aaaagccaac tataggaagt aaaaacaatg aaagtttcag
     7141 ccattcccgt ttcgagagaa gaaagagtcg tttttttaac ttctttactt ctttgaaaga
     7201 aaagtcttgc cctagcaaga tctggacgaa actgataccc aaagctacta cagtaaaagg
     7261 aagaagccag atagaatctt cgcgaatgaa ccaaaagaaa ggaaaagcga cagaaagccc
     7321 taaaaaccat ggcagcaatt ttctgctaga accgtatttg cggtaataaa tagctaataa
     7381 acaagcgata atgatctcaa cagccggaat cacgagggca ttacgatatg ttcgagtgga
     7441 atattcatgt gtaaaactaa tcggagaaaa caaaaaataa aaatagatac ttaaaagaag
     7501 atacttgttc ttcacaatcg gtttaaacgc gagtgcaacc aatatactgg caaaaatact
     7561 gaacatgccg aacaaaactg cataagaaag atgcagttta tgtgcaagta ctaaaaataa
     7621 gctgtaggag attcctttgg aaagtgttgt agtcgtatac tcccctagcc atttaccatc
     7681 caaaatattt tgagcatagt gaatcagcag ctgatcatca taggtcgccg acatatctgt
     7741 tccatagatt gctttactat ataaatacaa ccgaataatt gtaaataata cggctatgcc
     7801 aagaataaag tatgtatgtt tcttttttag ttggtcgatc aaggggttta aaatgttcag
     7861 catcttttcg tcctttcaat cacgttttgc tacttgtttg tgaaactttt cgttttttac
     7921 ttttctatca gtataccctg tttgtctgaa tttaaaaaga gggggcaaaa ggagcatggc
     7981 gaaaacgaat gcaaaaagca ctcctttgtt tcttaaacaa aaaaggacaa ggcagactgc
     8041 ccaatccttt tttgttgctg tcaggtagaa aacttaaagt gtgacttctt ctgctaataa
     8101 ctcagggaac acttcgggat cgaatgcttg cgctttgaac atggaaattt cttttccata
     8161 tggtgcgtaa gctttctttt tatctttacc gacatatggg gtttctaata ttttcgaaat
     8221 gttgtcaaac attggatcat ggacgatttt gttcaatgtg tcaaatccaa ttgttccaaa
     8281 accgatattg gcatgccgat ctttatgaga accttgtgga tttttagaat cattgatatg
     8341 cagtacttta agccgatcta atccgatgac cttatcaaac tcttccatta cacctgaaaa
     8401 atcttctttt atattatatc cggcatcatt cgtatgacat gtatcaaaag taacggataa
     8461 tttttcattc aattttactc catcgatgat ttgcgctaat tcctcaaatg ttcgaccgat
     8521 ttccgtacct ttccctgcca tcgtctccaa agcgatctgt gccgtctgtt cttttgtcaa
     8581 aacttcattc aacccctcaa taattctagc aatcccagct tctgcccctg ctcctacatg
     8641 agcgcctggg tgaagggtga tttgagtagc acctaaagct tccgcacgga caatctcttc
     8701 tctcagaaat tgtacagcaa atggaaacat ttctggtttg atcgtatttc ccaaattaat
     8761 gatataaggc gcatgaacga cgatatttgt tagtccgttt tctgccataa atgcttttcc
     8821 ggcatcaata ttcatctctt cgatagcttt tcttctagta ttttgaggag cgcctgtata
     8881 gatcataaag gtcgaagatc cgtaactggc tgcttcttct gccgcgccta acagcatttt
     8941 ctttccgctc atgctgacat gtgagcctaa taacatggat ggattcctcc ttgaaaggca
     9001 attgtttcga acaattcctg tatattgata agatgatgta caaagaaaat ggtttgtttt
     9061 tctttgccct gttctccata gtaactgaaa ctttccctaa agaaaagctt aagaactctt
     9121 tcataaaaaa gatcatttcc tcacgtttta tctcattcga aaaagaaatt cctgtcaaaa
     9181 acagccttgt ctgttttttt gtcctattat ttgctataat tagcgaaata aaaccattag
     9241 ttgaggtgac ccttttgcca cgcaaaaata ctcgaaaaaa acagaataga agaaaaaaag
     9301 aaaaatggtt tgttcctaag attattttta gagtgtttca atcattgact gtttttatta
     9361 ctgttcttct tttgttgttc gcagctctag gtatcggaat aggtgctggc tactttgcct
     9421 atcttgtcga agacacacaa ttgccgacta aaaaagaact gcagacagaa ctaggaaaca
     9481 ttacggaaac gtcaaagatt gtttatgcag ataatacaga gatatcaaaa atccagacag
     9541 atctgatgcg gacgactatt tcatctgata agatttcgcc tttattgaaa accgcgatta
     9601 tcagtacaga agatgaatac ttcgataaac atcaaggtta tgtccctaaa gctgttttac
     9661 gagcactagt ttcagaagcg actggcatcg gctcgtctgg aggatccact ttgacacagc
     9721 aattagtcaa acaacagatc ctaaccgatg aaacaacatt caaacgtaaa gcaaacgaga
     9781 tcgttctttc tgcacaagta gaaaaacact tttcaaaaaa tgagatcatt actacgtatt
     9841 tgaatgtctc accatttggt cggaataata aggggcaaaa tattgctgga gtccaggaag
     9901 ctgctagagg aatctttggt gtagatgcaa aggatgtgac tctgccacaa gctgcttata
     9961 tcgctggttt gcctcaaagc cctatcacgt atagtccata cacgaataca ggcgcgctaa
    10021 aagaagatct ttctgccggt cttgccagaa aagattttgt tttattcagt atgtaccgtg
    10081 aaggacaaat tacgaaagaa caatacgaag aagcgaaagc ctatgatctt acaaaagact
    10141 ttctcccaca acaaatcgcg gaacagactg acagagaatt cttgtattat accgtgatga
    10201 acgaagcaac aaggattatt gcacaacaat tggcagaaaa agataatgct gatatgtcag
    10261 attcatcggt cagtgaagct tattatcaaa aagcacagca aacgatccaa aacaaaggat
    10321 atacgatcca ttcgacgatc gataaagata tctatgctgc attacaaacc ggcgtagaaa
    10381 attatggcta catgttagat gatggtgctg gtagccaagt ggagactgga aatgtattga
    10441 tggataacag aaccggacgt atctatggct tcgtgggtgg tcggaattac agccagaatc
    10501 aaaataacca cgcctttgat acagaacgtc aagctggttc atccatcaaa ccggttcttg
    10561 tttacggtcc tgcaatcgat atgggacttg tcggatcaga atcccgtgta tcagattatg
    10621 caacgacttg gcaagaagga aaaaatgctg gtgagaaaat cgtcaatgcg accaatgaag
    10681 ggtcgaacac cttccaaacc gtccgtgagt cactggaatg gtccaacaat atcccagctt
    10741 atcacttata tcaagatgtg ttgaattccg gtggatcgaa acaatacgct tacgagcact
    10801 acttggcgaa gatgaactac ccagcaaatg ataattgggg cgttgaatcg gctcctttag
    10861 ggacagtaga tgtaacgacg cttcaacaga caaatggatt ccaggcctta gcaaatggtg
    10921 gagtctacga agaaggctat atcattgatt ccatcacaga taatgcaggt aatgtgattt
    10981 ataaacatga aagtaattct gtccgtattt attccgaagc aacggcatcg atcatgaatg
    11041 acatgatgcg tagtgtgatc aatgaaaaaa tcactacacc attcaaagat gtcatctcaa
    11101 gcttaaacgg aaatctagga aatgcagatt gggtcggaaa aactggttct accaatgaat
    11161 accgagattc atggctgatc gtctcaactc catctatcac tatcagtagt tgggcaggac
    11221 acgatgacaa tactggaatg gacagccaag caagaatacg ttcggctaat tatcttgcca
    11281 atctaatcaa tcaagcttac caagcaaacc ctgagatttt cggcactgat gagaaatttg
    11341 acttatcatc cgatgtgatg aagaaaaaag tatctgggtt cacaggacaa acagctggaa
    11401 aagttactgt caataaaaaa tccatccaaa cgccaggaaa aacagtggag tcactttggg
    11461 caaaaaatgg tccgaagaaa agcgccttta aattcggcgt aggcggtact gatgaaaact
    11521 atacagatta ctggaacacc gcttcagctt atgctagagc gaatccagag accaaagaat
    11581 cagacgataa agatgattga tctaatagat taatcagaat taaagggagc tgcgacaaaa
    11641 atcttgtcac agctcccttt ttccgaataa atggtgttca aaagcttaca tctacgatat
    11701 taagacaaat ttatgtaaaa atgtgaaaaa ccagagagta taaaatattt tgtgtaaatg
    11761 aaaaaatcca tacaaaaaag gaagtc
//
LOCUS       DAHUVV010000085        11591 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.088, whole genome shotgun sequence.
ACCESSION   DAHUVV010000085 DAHUVV010000000
VERSION     DAHUVV010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.088"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(322..2331)
                     /gene="metG"
                     /locus_tag="NK767_002199"
     CDS             complement(322..2331)
                     /gene="metG"
                     /locus_tag="NK767_002199"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCH6695180.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2599..2877
                     /locus_tag="NK767_002200"
     CDS             2599..2877
                     /locus_tag="NK767_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695181.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2974..3795
                     /locus_tag="NK767_002201"
     CDS             2974..3795
                     /locus_tag="NK767_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCH6695182.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4080..5090)
                     /locus_tag="NK767_002202"
     CDS             complement(4080..5090)
                     /locus_tag="NK767_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6695183.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5102..6055)
                     /locus_tag="NK767_002203"
     CDS             complement(5102..6055)
                     /locus_tag="NK767_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6695184.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(6045..7115)
                     /locus_tag="NK767_002204"
     CDS             complement(6045..7115)
                     /locus_tag="NK767_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6695185.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(7121..8164)
                     /locus_tag="NK767_002205"
     CDS             complement(7121..8164)
                     /locus_tag="NK767_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6695186.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(8165..9106)
                     /locus_tag="NK767_002206"
     CDS             complement(8165..9106)
                     /locus_tag="NK767_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6695187.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            9190..9537
                     /locus_tag="NK767_002207"
     CDS             9190..9537
                     /locus_tag="NK767_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCH6695188.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9607..11268)
                     /locus_tag="NK767_002208"
     CDS             complement(9607..11268)
                     /locus_tag="NK767_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCH6695189.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3287 a         2340 c         2100 g         3864 t
ORIGIN      
        1 tatagatgta ataaagtcat tgcattcaaa ctttatgaat gtaaggacaa taaaaaagag
       61 taccagatga atcaattaaa atctgcccga ccctttatca ttatgctttt tttctgtttt
      121 tacgattcct attagtatta ttctaaatta acaaaaatca gcgaagctcg tacttctttt
      181 tctaggattt attttggata gttctctgtt tctgtattat atgattggct tcaagtaata
      241 agaaggaatt tacgaaaatt gcttctcaaa tttcggaaaa agggggtatg acacaactag
      301 tgtcataccc cctttgaatt tctatgcgat ttctgagcca tttggcatgg attttggtgc
      361 ttcgattact tgtaaagagc catctggtgc ttctgcagaa aggatcattc cttgactgat
      421 ttgtccacgc atctttcgtg gtttcagatt tgctacaatg acgacttttt tcccgatcaa
      481 ttcgcttgga tcaggataaa attcagcgat tcctgacaag atttgacggt cttggctgtc
      541 tccagcatct agtcggaatt gcagcaactt atctgcccct tctacttttt gacagttgat
      601 gacttctgct acttttagtt ctactttttc aaatacatca aatttgattt gtttttcttt
      661 cgttgaaaca agttctgttt cttctggatc ccatttgact gtatcttcgt ttgtttgagt
      721 accttcagac attttttctt gaataaaagc aacttcttct tccatatcta aacgtgggaa
      781 gattggtgta ccttttgcaa ctacttttgt tcctgaagga aattcgccaa agtgtaatcc
      841 ttcaagattc attgtttcag agtccaatcc tagttgattg aagatcttct ctggcgtttc
      901 tgtcatgatt ggctgaagta atatcgctgc gatacgcaaa gattcagcta aatggatcat
      961 gacactattc aattcagctt tcttttcttc gtcttttgct aatacccatg gttctgtttc
     1021 atcaatgtat ttgtttgcac gagaaatcag cacccagatt tcagccaatg ctgtattgaa
     1081 ttccattttt tccattgcat catggtaacg tccgacaacg ttagcagccg tcgtagacag
     1141 ttcactatca aatggagtga caagtgaggc atagtcagga acgagtccat cacaatattt
     1201 gttgatcata gcaatcgttc gattcaataa gttccctaga tcatttgcca gatcataatt
     1261 gacacgggaa acaaaatctt ctggcgtaaa gacaccatct gacccaaacg gaacagcacg
     1321 aagcaaataa taacggagtg catctaaacc ataacgttcc acaagcattt caggataaac
     1381 gacatttcct tttgatttag acatttttcc gtctttcatc aatagccagc catgcccaaa
     1441 tactttttta ggtagaggca aatcaagggc catcagcatg attggccaat agatcgtatg
     1501 aaagcgaacg atttctttac cgaccatctg aacatctgca ggccagtatt tttcgaatag
     1561 actttcatct tcggagccat atcctagtgc agtaatataa ttggataatg catcgatcca
     1621 tacatacacc acatgtttcg gatcactctt gactggtact ccccaagtaa aagttgttcg
     1681 tgaaactgca agatcctcta atcctggttt gataaagttg ttgatcattt cgtttttacg
     1741 agattctggc tggatgaatt cgggatgttc ttcatagtat tccaatagac ggtctgcgta
     1801 tttgctcatg cggaagaagt atgattcttc ttttaccaat tctacttcgt gtccacttgg
     1861 cgctttacca ccgatcacgt taccttcgtc atctttgtac acttctgcaa gttgtgtttc
     1921 tgtaaagaat tcttcgtcag aaacagagta ccagccttca tattcaccta agtaaatatc
     1981 tccctgttct aataaacgat caaatatttt ttgaacagct gctttatggt aatcatctgt
     2041 cgtgcgaatg aatttatcat aactgatatc aagcgttttc cataatttct tgacatcagc
     2101 cgccatttta tctacatatt cttgcggttt tacacctagt tcttctgctt ttgtttcgat
     2161 tttttgacca tgttcatcca ctccagtgag ataaaacaca tcaaagccca tcaaccgttt
     2221 ataacgagca attgcgtcac atgcaatcgt agtatacgag ttgccgatat gaagtttgcc
     2281 gcttggataa tagattggcg tagtgatata aaaagtttcc ttttctgcca cggggctctt
     2341 cctcctttat cgcatgatgc gaattcgttt ccttgctagt ataccataaa aaaagcatgc
     2401 tgttagcttt ttttcaaaat gaaaaagact ggataaacga cttctatcta cgttcaatct
     2461 atcctttaaa aggatgaata ggttgaataa atgcgggttt ttgttttgtt tcatatctac
     2521 taaaaaacgg ctttttattt ctcccctcac acgtggtata ataaagacta tctatcttag
     2581 taagggactg gaacaatcat gcagaagaaa catctttttt ttacacttag tattgctttt
     2641 ctttcattag cacatttaat attcagctat ttttatattc gtatgtatgg ttattttaac
     2701 ctccatgggc atttgaacag ttttatgaca gcagcttgga ttttgcgttt tatcattgac
     2761 gtatatatcg ttatttgtgg cttctttgcc attcgtgaag agcggtataa agtattgccg
     2821 ttttatctgc ttttcttttt attcaatctc atacttccat ttatttttca tatttaaatg
     2881 ataacctaga tttttcaagt aaatccttgg ttttttttca gagtctgcta caataaggta
     2941 aaaagtactt ttactgtcaa tggaggtggt cagatgaacc gttttgcttc taaagaagaa
     3001 gaaaaattat attatgaaca taaagcaagt gaagaagaat ttcttaactg gtatcaaaaa
     3061 caggaacgtc cagaatatga caagccctct ttgactgtcg atatcgtact gatgtgctat
     3121 aacaaagaag aagatcagct gaaagttttg ttgatccaaa gaaaagggca tccttatcga
     3181 aattcatggg cattgcctgg tggctttgtc caaaaggatg agtcaactgg agaaagcgtc
     3241 ttaagagaaa caaaggaaga aactggtgtg gttatttcaa aagaaaatat tgagcaactg
     3301 catacattca gtacgcctaa tcgagatccg cgaggctggg tcgtcacagt cagctactta
     3361 gcctttattg gagaagaacc gctaatcgcc ggagatgatg ccaaggaagt acgctggttc
     3421 acactagaac gtcatagtaa caagatccat ttatctagcg gagaagtggc tatctcgctt
     3481 gatttagtca ctggagagtc ttctggcaaa gatactttgg cttttgacca tagtcaaatc
     3541 atcttgaaag cattcaaccg tgtagtcaat aaaatggaac acgagccgca agttctacaa
     3601 gttttaggta aagatttcac gattacggaa gctcgaaaag tatttgctaa attcttaggg
     3661 atcgattata aaacgatcga tcattccaat tttaagaaag ctatgctcca atatttcgat
     3721 gagatcggtg aacgtcctgt aggaatcggc cgtccttcaa aaatctatca gctcaagccg
     3781 gggcaccatg aataaggttg tgagcaaaag attcagacct aatcattcaa gaaaaaacgc
     3841 aaaaacaact ctttgtgagt tacgtttttt gtttcagaat tttgatgtat tcgtaaactt
     3901 tctgaataaa aaattaacaa tagcagaaaa agctcgtacg aactttttct gctattgttt
     3961 tattggtttg tcataattag tgagcttttc ttcaaaagaa aaattcctag aaacaaaagt
     4021 cattaagact ttttattaac aaggctaacc ctaacgctcc tatcagtttc attcattttt
     4081 tatcccttga taaccgaatg tctgcccatt ttttctacta cttggctctt tcttacggaa
     4141 ttgctttctt ctgctccgtc ggtattgatc aaaagaaatt ttttattata ccattgggac
     4201 ttataacgca attttgttat taatttatgc cagtcgtgaa tagaaaaatc agcatgtgcc
     4261 tcttcttttg gaaaagattc tacaaataag tagtcatatt gtttgaccaa tttcccagat
     4321 aattgttcaa gataatcttc tcgctggttc ttttgtttca gataaagatc cattactttt
     4381 ctcttttgtt tctgataatt gcttgcctga tctaacctga tctttctatg gtgagcggag
     4441 cggactttca cattcaattt ccgctgtgca tgttgcagct tttcttctgt ctggtgaaat
     4501 tttggtaaag ttacttctag aggtcgagat gtttggatca atgtcttcgg atgataggcg
     4561 atcccgatcc atttagctaa agattctttt tttactggcg aaacatcaca aagtagtgcg
     4621 acataaaatt cttcattata tcgagcagaa atcgtagcgg agcgaatcga tccttcaact
     4681 ggccgatgct gatttatctt gattagttct ttttgttttg gtaactttaa gtagccattt
     4741 tccagataga tcgtacgggt ttggttgttg gtcgtataag attgccacat actttttttc
     4801 gttttaagct ttgggaagct ggcacgtccc ttgaaataat tgcgaaaagc ccgatccaaa
     4861 ttaagctgtg catttgctaa agcaagactg tctacttctt ttaagaatgg gtattctttt
     4921 tttaatgtag cgggtgtgag cgaaaaattg gctactcctg tttgttgcca acttttctgc
     4981 cgctttttca gcaaatggtt ataggtaaat cgagcacatc caaaagtacg aatcaattgc
     5041 tgtttttgtt cttctgtcgg atacatacgg aatttaaaag cttttagcat ctatgtccct
     5101 cctagttagc aaccagaaga gattcctttt gtcgttttag tttttttgcg taatatggga
     5161 cttcctgctc agttgcatag acaaaatgtt ctggtgcgat ttcacgaagt tgacgtactt
     5221 gtccgtcatc tggttcaggt tgatatttga ttcttctgcg ttttctctca tggtctggat
     5281 ctggtagagg aatagcagat aacaaacttt ctgtataagg atgaacaccg tgattataga
     5341 tttcatcact tgttcccatc tctaataaca agccgttatg cattacaccg attcggtcac
     5401 taatatgttt aaccatcgac aaatcatgtg caataaatag ataggtcaat tgatgttctt
     5461 tttggagatc ttgcaacaaa ttgaccactt gggcttggat cgacacatct agtgcagaaa
     5521 ttggttcatc acaaatgata aaacgcggtt ttactgccaa tgctcgtgca ataccaatac
     5581 gctggcgttg cccacctgaa aattcatgag gataacgagt accgtggctt ggattcaatc
     5641 caacagttct caatagatca tcgacttttt ttgcacgctc ttcagatgat ttagctagtc
     5701 cattaacatc aattccttcg gcaatgatat ctctgacttt catacgagga ttcaacgaag
     5761 cgtatggatc ttgaaagatc atctgaacat catgccggaa tttcgtcaaa ccttttttat
     5821 ctttgatctt cgttatatct tgaccgtcga ataatatctc accatcagta ggttcattca
     5881 agcgaatgat cgtacggcca gtagtggatt ttccgcttcc agattctcca actaaaccga
     5941 atgtttctcc ttcataaatg tgaaagctga tgtcatttac agcatgtact tcattctttt
     6001 ttcctacatt gaaatactgt ttcaatcctt taacttcaag tagtttattt ggcatcagca
     6061 attcctcctt gaccgtaagt atgtttttgt ttctctttaa agatagccca gcgacgttgg
     6121 atttcttctg gcggtgtcac tttgggtgcc tgcggagcaa gtagccaagt tgctgccttg
     6181 tgtgtttttg atagttcaaa aaatggtggt tcttgttctg catcgatttt catcgcgaat
     6241 tcatttcttg gatagaatgc gtctcctttt ggcgggttca ataaatccgg tggagaacca
     6301 ggaatagcat acaatcgatc gttcgcacta tctaatgtcg gcattgaacc aagcagtccc
     6361 caagtatagg gatgttgcgg gttgtagaaa atttcttccg atgttccaac ttcaacgatt
     6421 cttcctccat acatgacagc gacacgatca gcgacattcg ctactacacc aaggtcatga
     6481 gtgataaaga taattgatgt atcgattttt gtctgaattt ctttcagcag ctccaaaatc
     6541 tgtgcttgaa tagtcacatc aagcgctgtt gtaggttcat cagcaatcag gatttccgga
     6601 taacatacca gtgcaatcgc gatcacgatc cgttgacgct gacctcctga gaactgatgc
     6661 ggataactgt tgatacgttt ctcagcttcg ggtattccaa ctaatttaag caattctaat
     6721 gcagcttttt gtccctcttt ttttgaaact ttattatgct ttctaaggga ctcagcgact
     6781 tgcttcccaa tgggcatcgt cggatctaaa gaggtcattg gatcttggaa aatcatcgct
     6841 atttctttac cacggatttt ttgcatctct ctttctgatt ttccaacgat atcgttacct
     6901 ttaaagagga tttgaccagc atctatattg gcgttgcttg acagcaagcg catgatgctt
     6961 ctggtcgtca ctgattttcc acttccagat tccccaacga tcgccaatgt ttctccttta
     7021 ttcaaatgaa aattgacacc gcgaatcgct cgtacctttc cagcatacgt atcaaaagag
     7081 atttctaagt ctttcacttc taaaattttc gtcatcgtct tcactctttc attttaggat
     7141 caaaggcatc acgcaatcca tcagcaagta agttaaagcc aatcatgata accgataaag
     7201 ttactgccgg aatccataat agataaggca gatacaggaa tgtcttgtac ccttcgctca
     7261 acatcgttcc taatgaagct gacggaggcg taagacctaa accgataaag cttaaaaagg
     7321 cttcgaagaa aatagcagat ggaatactaa acatcatttg aataataatg acactagaga
     7381 tattcggcaa aatatgtttg aatgcaattt tcattcttga ttcacctaat gtcaatccag
     7441 ctaaaacata ttcttgatct tttaatttca ttgtttgtgc acgaacgata cgtgccattg
     7501 gtatccagtt tgtgatggcc atagcagcca cgattgaaaa aattcctggt tcaaaaacga
     7561 ccaacatcaa aatcatgaca acgaggtttg gaattcctga taagatttcc aaaaaacgtt
     7621 gcatcacgtt atcgacacgt ccgcctaaca aaccagaaac caacccgtaa gctacaccaa
     7681 tcgttacatc tagtaatgca gcaataaatg cgatcagcaa cgagattctc attcccataa
     7741 ataagcggct taatacatca cgtcccaaac cgtctgttcc taaaaagaaa tttacgtttg
     7801 aagggacgtt tgcttgagca tatttatcaa caagttcacc accaaccatt gcttttccat
     7861 tcaatccatc gatattgatt cccggaatac gaggaggcaa attgatataa gccacgtttt
     7921 gggcagttgg atcatgagga gaaacaaaga ttgtgatcac agagatcaaa atgatgacca
     7981 atagtaaaac cattgaaata acggcagctt tgtttttctt taaacggcgc catgaatctt
     8041 ggataaaatt caaagaaggt gcagtaatct gttcacgttc ttcttcggta tttttttgta
     8101 atggaagaaa ttcatccgca gggatatctg caactgtttc ataacgcttt ggtatcattt
     8161 ccatttatcc tcggctccct tctgaaacac gaattcttgg atcaactaga ccgtacaata
     8221 gatccacgat caaaataaca aaaatcagca taaatgagta aaggatcgtt acagccatga
     8281 tcgtaggata atcatttgtt gtgattgact taacgaattg ttccccgata ccaggaatag
     8341 caaagatatt ttctaccacc agtgatccgg tcatcaatgc tacagctaac ggtcctaata
     8401 aagtcatcaa tggaatcaaa ctatttcgaa gaccatgttt aaaggcgatt tcccaacggc
     8461 tcaatccttt tgcttttgct aattccacat aatcactatg cagtacttcc accatttccg
     8521 tacggataaa ccgagcagag tcagcaagcg gcgacatggc caacgcaatt gttggtaaga
     8581 ttgtgtacac aaagccatcc cattttgcga ttggcaaaac ttgtaatttg attgcaaaga
     8641 tatattgtaa taaaacagca aatacaaagt ttggaatgga acgacctaat atcgctacga
     8701 gtgtagaaga tgtatctgcc catgtatttt gcttcatggc agagatcgtt cctaaaatcg
     8761 tccctaggac tgttccaaag ataattgcct gtaaccctaa ctgcaaggat ggtccgattc
     8821 ttccagctaa caaatgtgcg actggctggt ttttaaattg gaaggaaatt ccaaaatctc
     8881 cttgaagcaa attagaaaga taaatcccat attgtacgat aactggttta tccaaaccga
     8941 cttgtttatt tagcatctcg attgtttcag gactcaaacg ttcctgattc gtatatggcg
     9001 tgcccggcag caattgcatc aagaaaaagg tgattgttgc aattaaccaa agcgtgatga
     9061 tcatgaaaaa gacacgtttt aatacatatt tgatataact attcactaat gtttccccct
     9121 agcttttgac ttctgacaga tgtttcgcta cattattaga taatgaacca aaaataaagg
     9181 agcaatcaaa tgaaaaaaaa atctatcccc tacgcagtag cttttctact gattctggtt
     9241 attttaataa aaaatctcat caaccattcc tttacactca ttcaattatc taatgatcta
     9301 ttcttatggt cattgccgtt tctgattatc ggcggttttt tatgggtctt ttcttcgggt
     9361 ttcttcgatc attttcagcg atcggtccat cttgcacgaa caaggaaccg aaagaaaaaa
     9421 ccagaatttt cctcgctctc gtctgccagc tacggtatgt acagcttttg gctgatcatt
     9481 gccggaatac taattgctct ttctgctatt ttcatgcttt tttctttgtt aggataagtg
     9541 aatatgagaa cgaggttgcg acatttgccg cagcctcgtt ttttctcatg ctcacggtat
     9601 taacgattat tcttcaacgt atgtccattt gtaatcccat gaagcgcctg ccgcatggtg
     9661 aacgatacct ttgatttttt cattcactag gtgaccttct gctttttggt acaccggaac
     9721 gacacccata tcatcagaga tgatcttgat tgctttttgg aaatcatccc aacgggcttg
     9781 ttcatcgcca gcgtctgttg atgaagctgc tttaactgct tcgtcatatt ctgcattgct
     9841 ccagcgacca cggttgtatg aattacctgt gacaaacaaa tcagtaaagc tgcttgggtc
     9901 tgcatagtca gcagcccatc cgcctagtac tgcgtcaaaa tcacccgaag ttgagcggtc
     9961 aagacgtaca gagaatggaa caggtgttgg tttgactgtt acgccatcca agttttcttg
    10021 gattgagttt tgaacatatt cgattacttt tttcgatgaa tcatcatcag aagccatcaa
    10081 atcaaattct aaagaatcaa cgcccaattc ttttttcgct ttttcccaat attcttttgc
    10141 tttatctttg tcataagaaa ctaattttcc agcttctttt gcaaagtctt cgttcgttgt
    10201 tggacttttt accatatctg atgggatcaa tcctgtagag acaactgatc cgtcacctag
    10261 tacttgtttt actaaagctt cacgatcgat agaataagaa atagcttttc ttaggttcac
    10321 attgttgaat ggtgaattct tttcacgttg attgaattcg atatagcttg tccgcgcttc
    10381 tttaacagaa gtataagctg gatcgtttgc gttttgctgt gctaactcac cagacaagat
    10441 cacgtcatct gcttggccat ctttgaataa gttcaatgca gtagaagatt cttttactac
    10501 actgactttg atttcagaaa gttttactgt atctttgtcc cagtaatgat catttttctt
    10561 ataagtccat tctgtatctg ttcctggtcc atcaaaatct tctaatgtga atggtccatt
    10621 ataaacagct ttatcacttg cagatgcata ggcatcccca ttttcttcca ctacggattg
    10681 tttttgtggg aaaaatgatg ggaaagcaag cagatattgg aaatatggtg tttgtttcgt
    10741 caatttgatt tctaattcat agtcaccaac tgctttgatc cctagttctg atttatcttt
    10801 ctttccagct gtaatgtctt ctgcattttc aactggcgca tacaggtaag catattcaga
    10861 agctgtagct gggtctactg ttcgttgcca gccataaacg taatccgctg ctgtaacagg
    10921 atcaccattt gaccattttg cgtcttctcg taatttgatt ttgtaagtta atccgtcttc
    10981 actgacttct gccaactcgc tagctcctgc cggctgtggt ttgctgtctt catcaagacg
    11041 gtaaattcct tcataaacgt tattcaatgc tgtaaaactg attgtatctg ttgcaactga
    11101 taagtcagct gtaggcattt cttgaggcac gactaagtta aatacgcctc ctccatctgc
    11161 tgaaccgctt tttgctgaac tactgcttgt ggctgaactg ccgccataac atccagcaag
    11221 tacaaggccg cataccgcaa ccaaaccaaa tgtaaatttc tttttcattt tacgtacccc
    11281 tttattggaa ttttcagaat aattaatatt ttctgaagtt gtttaagagt ttaatgaata
    11341 attattaaaa aatcaacact tttttttgtc tttttcataa aataatgatt ttttcgtgag
    11401 gtttcaagga atttttcaga aaaaatagct ttgtacaagg ttttccatgg tcttccaaaa
    11461 gccattaaat aattttcgtt atattgtcta ttattgccat acaaagataa aaaagaataa
    11521 acaaaaagaa actgtgacaa aagtcttgtc acagtccctt atctgaataa acggtgttca
    11581 aaagccagtt t
//
LOCUS       DAHUVV010000086        11562 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.089, whole genome shotgun sequence.
ACCESSION   DAHUVV010000086 DAHUVV010000000
VERSION     DAHUVV010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.089"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(62..2137)
                     /locus_tag="NK767_002209"
     CDS             complement(62..2137)
                     /locus_tag="NK767_002209"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCH6695190.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2139..3056)
                     /gene="glyQ"
                     /locus_tag="NK767_002210"
     CDS             complement(2139..3056)
                     /gene="glyQ"
                     /locus_tag="NK767_002210"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCH6695191.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3440..4252)
                     /gene="recO"
                     /locus_tag="NK767_002211"
     CDS             complement(3440..4252)
                     /gene="recO"
                     /locus_tag="NK767_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCH6695192.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4395..5294)
                     /gene="era"
                     /locus_tag="NK767_002212"
     CDS             complement(4395..5294)
                     /gene="era"
                     /locus_tag="NK767_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCH6695193.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5309..5704)
                     /locus_tag="NK767_002213"
     CDS             complement(5309..5704)
                     /locus_tag="NK767_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HCH6695194.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5688..6164)
                     /gene="ybeY"
                     /locus_tag="NK767_002214"
     CDS             complement(5688..6164)
                     /gene="ybeY"
                     /locus_tag="NK767_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCH6695195.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6181..8373)
                     /locus_tag="NK767_002215"
     CDS             complement(6181..8373)
                     /locus_tag="NK767_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HCH6695196.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8397..9368)
                     /locus_tag="NK767_002216"
     CDS             complement(8397..9368)
                     /locus_tag="NK767_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCH6695197.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10073..11452
                     /gene="brnQ"
                     /locus_tag="NK767_002217"
     CDS             10073..11452
                     /gene="brnQ"
                     /locus_tag="NK767_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCH6695198.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3111 a         2429 c         2122 g         3900 t
ORIGIN      
        1 aaataaacca gagaaacagc ctattttgtg cctgttctct ggtttattta catattagaa
       61 attattttgt gatgatttga tctaaacttg ctagtgacaa agccatggca gcaatttgac
      121 ttaattgcgt taatcggttc ttacgtactg ctggttcttc tgccatgacc atcgtctgat
      181 caaaataatt ttcgatcagt gggcgaaggc ttacaagctg atcataattt tcgttgatcg
      241 tttggtcagc aaagcttgtt ttcattgcct ctactgcttt gtgcagttct ttctccgctt
      301 cgttttcgaa cagttcaggg tctacttctg attgtttttc aagctccgct tttttcgcta
      361 agttcacgac acgtgtcaaa gcttccatcg atggtttgaa gtcttcatct gcaagatgct
      421 gcttgaacaa ttgtgctgct gcaaatactt ttgtcagatc tttttgttct gcgttcaaaa
      481 cagcttcgat aacatcatga cggatattct tcgttgtcaa taattggcgc agacggcctt
      541 tgataaagtc gatcacctgt tgttgtcccg ctgacaagcc aatgccaaaa cgatccgtat
      601 ctttactgat aacttcacta atcgtttctt gcagaacaga caaaggaaag gcccattttt
      661 tcgattcgat gattcgaacg acaccatatg cttgacggcg caaagcatat ggatcgtttg
      721 agccacttgg gatcatccca actgcaaaga aagaaaagat actatctaat ttatcagcaa
      781 tcgctaaaac agaacctact gtagtctcag gtagatctcc ttcactggat ataggcatgt
      841 aatgttcacg gattgctgtt gctacggctg gtttctcgcc tttaagcaga gcatattttt
      901 ctcccataat cccttgtagt tcagggaatt ctcctaccat gttggttact agatcaaatt
      961 tatagatttc agcagcgcgt tgcagatcca tcaattcttc ctcagatagt cctacttgtt
     1021 cgcctatgat gcgagcgatg acttgtaccc gttgcatttt ctcatagatg ctaccgattt
     1081 tttcatggaa agtaacagtt tttaattttt caacgtattg gtcgattgaa gctttcttgt
     1141 cttcattata aaagaattcc gcatcttcta aacgagcagt caatactttt tgattgcctc
     1201 gtaccacatt ttccaatttt acccgatcgc cattacgagc agaaataaaa tgaggaagca
     1261 acataccttg atcgttccgt acttcgaaat aacgttgatg ttctttcata gaagtgacta
     1321 gtacttcttc aggaacagaa agatattttt cctcgaagtc tccagaaaag acagttggat
     1381 attcgactag attgttgact tcttctagta aatcttcatc tagatcaatg atccagtgat
     1441 ttttttctgc taattcgttt gcttgtgtaa cgatcatttc ttgtcttttt tgcggtgaag
     1501 caataacaaa ttgttcattt aatttttctt cgtattcttt tgcatgcgca aatgttacat
     1561 cttcgcctaa gaaacgatga cctcttgaag ctctgtctgt tttgacatcc aagatctcaa
     1621 acgggattat ttcgtcatct aacaatgcca cgatccagtg aatagggcgg atatattcaa
     1681 aatcgtattc tgcccaatgc atcgttactg ggaaagtgag gcttttaatg acatctgcta
     1741 aacctgtcaa tacttctttt gcttctttgc cgtgagtgta tttcgttaca tagacatatt
     1801 ccacgccttt taattctcta aaagtgatag catctgtcgt cagaccttgt ccgcggacaa
     1861 atccttcagc tgctttcgtc cagtttccat ctgcgtccaa agcaattttt ttggctggtc
     1921 ctttgacttc ttcttcgctg tccgcttgtc gttctggaat atttgttact ttgattgcta
     1981 aacggcgcgg tgtggaaaat gtttcgatcg tatcataagt caattggttt tcatctaaaa
     2041 attttgctgt tttttcttct aattgtttca tgcttggtgt gactacatga gcaggcattt
     2101 cttccagccc gatttcaagt aataagtctt ttgccatctt attcagcctc cttcacgttt
     2161 tctggatttg ctgtgttttt attcaataaa gggaatccta atttttcgcg ttctgccaca
     2221 aagattttgg ctacagcacg agccatgttg cggatacggg aaagataacc tgctcgttct
     2281 gttacagata ctgcaccgcg agcatctaat aaattgaatg tatggctgca tttcaaaata
     2341 taatcatacg ctggatgaac aagattttca tcgatacagc gtttagcttc tttttcaaaa
     2401 cggtcaaaat tgcttagtag taagtcttgg tcgcttactt caaaagaata tttggaatgt
     2461 tcatattctg gttgtttgaa gatctcgcca tatttcacgc catcagccca ttctaaatca
     2521 tagacacttt caacttcttg gatataagaa gcaagacgtt ctaatccata agtgatctca
     2581 gaagtaactg gtttacaagg tagtcctccg acttgctgga agtacgtgaa ttgtgtgatc
     2641 tccatcccat caagccatac ttcccagccc acaccggcac aacccatcga tgggttctcc
     2701 cagttgtctt caacaaaacg aatatcgtgt tctaatggat caattcctaa aaggcgcaag
     2761 ctttctaaat aaagctcttg gatattttca ggtgaaggtt tcattacgac ttgaaattga
     2821 tgatgctgat acaaacggtt tgggttttct ccataacgcc cgtcagctgg tcggcgggat
     2881 ggctctacat aagcagcatt ccatggttcc ggtccgattg cccgtaagaa tgtatatggg
     2941 ctcatcgtac ctgctccttt ttctgtatca taagcttgca tcagcatgca gccgtttgat
     3001 gaccaaaatt tttgtaatgt taaaatcata tcttggacgg tcaatttttt actcatctgt
     3061 tttgcccctt tcaattttta agcgttgctg tcaataagaa tacaggaaag aaaacgacaa
     3121 aaagtcccta tgtctgatta cagacatagg gacgaataat ttcgcggttc caccctactt
     3181 ccgaataaag attcggcagc ttcatttatc gtactcgcaa gtgcctcttc aaaaatcgtt
     3241 tgggtccggc ttccactctc ccgaactcgc taaaccacgt gttttttact gatcttggtc
     3301 aatgtacgta ttcattttgc agttttcttg tttgtataat agatcctctc agtgaacgat
     3361 ctatttaatc tacggttaat aatagacgga agtccctgta tttgtcaaat agttttgatt
     3421 tttttgttgt atctttcatt tattcttctt tttcaggtat ctctttttca ggcatatttt
     3481 tctctggcgt ctttaatacg tgtgcccaag acttcatttg atcaatgaac tttttgcttt
     3541 ttagatgaat acctacatat tcatcataca actggtcaat gaggtcacgg attgcctgct
     3601 tcgtttcttc tttcaagtgg attccactga tcttatcata agaaatagcc gaaaacattc
     3661 gaacgaaata aacagcgcga ggatctgcat gataacggtg ttcgtccatt ggccagtgtt
     3721 tctcgcatat cacaccgcca tatttggaag aatagtcaaa ttttcctttt gtttccccgc
     3781 atacgacaca gtgcgtccaa attggcgcga ttccgaagcg ttgcagtatt tgaacttcaa
     3841 agatattcgt gatgatttct gcatcatctc cttggtccat ccgctgcaga gcttgttgcg
     3901 taaattggaa taaatgcggg tcatattgat gatcttcgat tgctgcatct actagattta
     3961 gcaaatacgt accataaccg ctcaaaaaga tatcttcttg gatgttccga aacgcatgga
     4021 cgtctttgac actgcttaaa aacgacaaac cttcacttcg aaaattaccg atatagactg
     4081 cttcggtaaa ggggagcaaa gccggagcta atggctgatt ttgccgatgg attcctttga
     4141 tataaaacat cattttgcca gaagattccg taaagatttt gactaattta tctttttctt
     4201 tgtaattacg gctagataaa atcaagcctt tcgattctgc aagctggctc atattctccc
     4261 tcctaaaaaa taaatctgga aaagcagatt ctgccgatcc agattattta tgttacgatc
     4321 ttccgctgtt tttctcaacg tacggatttt tatccagttt atttctatac ctaacctata
     4381 agaagtccag aatatcagta ctcgtctttt cggtagccga agtcttgcag atagactttt
     4441 ttgtctcgcc agtctttttg gactttgacc caaagttcta gaaatacttt gtctcctaaa
     4501 agattttcga tatcctttct tgctttcgtt cctacttgct tcagcatttt tccgcctttg
     4561 ccgataatga ttcctttttg gctatctctt tcgacgatga tcgtggcctg gatatggatt
     4621 ttatcattct cgtttcgctt catcgaatca acaacgactg cgacagaatg aggcacttcg
     4681 tctcgtgtta aaaacaagac tttctcacgg atcaattcag aaacgatgaa gtattcagga
     4741 tgatcagtca cttgatcatc tggaaaatat tgtgggccct ctggcatttc actgactaat
     4801 acatccatta agcgttcgac attgtttcct tctgtagcag agatcggaat cacttgtgca
     4861 aaaggcatct gcgtcgaata atcttcgatg attcccagca actcgtctgg atggatctta
     4921 tcgattttat tgataatcag ataaactgga gaatgcacgt tttttagacg ttcaataata
     4981 aaatcgtctc cccggccgcg tttttgatca gcactgatca taaataagac tgcatccact
     5041 tctttcaacg cactgtaagc tgcttctacc ataaaatctc ctagacgatg ctttggttta
     5101 tggattcctg gtgtatcgat aaaaatcagc tgagcttctg gtgtagtata tactccttgg
     5161 attttatttc tagttgtctg tgccttgtca ctcataatgg caattttttg acccacgatt
     5221 cggttcaata atgtggattt tcctacattc gggcggccga cgattgcgac aaaccctgat
     5281 ttatgttctg gcatatacgt tctccttttt aattaaacaa caaatgatat attttaggga
     5341 caaataaaat caacccaatg atgacagcaa aacaagcggt cactaaaact gctccagctg
     5401 ccatatcttt gatttttttc ccaatcggat gaaaatgaaa atccgtaaac atgtccacta
     5461 cattttcaaa aacagtattg atgatctcta ctatccaaac gagaaaaaca gctaaaagca
     5521 gccataacca ctctatccga tcaagctgga aaatcacgcc cataaataaa gcaaggatac
     5581 cgaacgcaac atgtttcctc atattgcgtt cttcctcaaa aacggttttg actccttgga
     5641 cagcaaattc cacggacatc aggaaatgtt tgtttttctc tacgttttta tcttttgagt
     5701 ccataggcat ccaagatctc tttctgtaaa ccaaacattt ctttctcatc ttctggtgtc
     5761 atatggtcat acccgttgat atgcaagaat ccatgtaccg ctaaaaagcc aagttcccga
     5821 tcatatgaat ggccgtaagc ttctgcttgt tcagcagcac gttcaataga gatcatgatg
     5881 tctcctagta cacgaggcaa tggattctct ccatcttcaa aaatcactgg gatttcgtct
     5941 tcgccttctt cttccagagc aaaactgatg acgtctgtcg gtgcatcttt tccacggtaa
     6001 tcacgattga ttacttggat tgccgcattg tccatgaaag taacagacat ttctgtttcc
     6061 tctggcagct ttaaataaga tgctgcaaat tgcagcaagt catcgatttc tttcattttc
     6121 tcttctgaga cttggtttgt ttcatccata aaggtaatat ccattataca tcgcttcttt
     6181 ctattcttct tgttcttcct ttatcttctc aacttctgtt ttcattttag gatatttgat
     6241 ccgagaatgg aaggtgctac ttaaaccgct gatcaatgat ttttccactt ttcggatttc
     6301 tttcaaggtc agtccactat cgtctaattg accatcggaa atacgctctt cgataagatt
     6361 atggacaaaa ctttcgattt tttcgctcgt cggatgatcc attgcccgta ctgcagcctc
     6421 acaactgtca gcaatattga cgatccctgc ttcacgactt tgcggttttg gtccaggata
     6481 acggaattgc tcttctgtta cttccggatt gcgttctttc gctttgatgt agaaataacg
     6541 cattagcgtg gttccatgat gctgtttgca aatatcgatc accatttgcg gcatattata
     6601 atcttccaat attttggctc catctgtcac atgtccaaag ataatatgtt tactgtcttc
     6661 tggcaataag aaattgtgcg ggttttccgc tcctgaaggc aagttctcaa cgaaaaagct
     6721 agcatgtttg atcttgccga tatcatgata atagcaagct actcgagtta gcaaagaacg
     6781 tccgccgatt tctgctacag cgttagcact tagattagct accatcatac tatgatgata
     6841 agtacctggt gcttcttcca gcaactgttt caataaagga tgatttggat tgctgagttc
     6901 atttaataca attacactgt catcattgac catcaattca atatatgggt gtagaccgat
     6961 cgtcagcaag aatgagaaga gtgcaccagt gaacccgcag acgagcatta accaagtcgt
     7021 tgcatcacca aagctcattc cttgatagac gatcaaaatg acatccatca tcacaggaaa
     7081 aataatgacc cacatggcag cagataatcc ttgttctgag atccgtttgc gtctgacaat
     7141 cgttgccatg agcccagaga aaacataaga catcaagata atcgttagaa agttcgtgcc
     7201 aatcgaatca taaaaaatga aaagtgcgga tactgcttgg aaaataccag ccatgatccc
     7261 tgctcttcga ttcaagaaaa agcttaaaac aagtggtgca aaagcagcgg gataaaacag
     7321 cgggatataa gtagcttgtt ccgtttggaa taattggaaa aatttcatca gcactatgct
     7381 gactgacata gctgtcacat agaaaagcac gtattttgtt ctttctcctg cattttggaa
     7441 ttgaagtgaa ttataaatca gtacgccgat ttgaagcaaa acagctaaaa ccatggcaat
     7501 tagcggaaaa atcgaagttg tttgattagt cagtcctagt aattccaatt ttttcatagc
     7561 tgaagcatcg atttgattac cttcacgaac aatgatttct ccttggaaga tcatcactgg
     7621 ttgtacagct tctcgtgcgg attgtttcaa ttcttctgtc tttttctcat tcaatacgtc
     7681 attcactacg ataccttgat ccactaagta gcgaatcatt tgttgttgtg ctggtgtgac
     7741 attcaaatat tggatttgct cttctgcttc ttgtttaaaa gcttccaatt cactttcacg
     7801 aacttgtttg ctcatctgtt cgtcaatgag cttcagactc tcatccctca cagtatggat
     7861 ctcagtttca gtcatttcaa atatcgtcga ataaaaattg ttcggtagtt tttgataaaa
     7921 cgcaacattt tctgcattca cattttcaaa atttttcttc agcgcggcga tccgctcttc
     7981 tgtagtaggc tttgggatat tttcatcttc ctttgcctta tcaatctttt cgtcataaga
     8041 tttgttgatg gcatcattcg tcttatcgat caattcaaat agttgcttga tccgattgtt
     8101 ctggtcatct gcaagatctt tttggtaagt gtattctgga gtgactgctt ctgctgccag
     8161 ttttcttttt tgttcggttt cttccgtgtt ctcgatcgtt ttgttcgcac ggatactttc
     8221 ttctgctact tggccttctc tgaaatcatt ggccttttgt ttgacactac taaatgtaac
     8281 gccgcagata atgataaaaa acaagaacag caggaaaggc aatgctgcct ttccaagttt
     8341 ttcttgtagt ttctgcagtg ttttattcaa catgtgctta tcctcctaag caaagcttat
     8401 tcttgatgga gatctgcctt ttcataagct ttgatgattt cggcaactac agggtgccga
     8461 acaacgtcac ttgcttcaaa attaacaaaa tcgattttct tgatttgctt taatgttcgt
     8521 tctgcatgaa caagaccact tgttgtccct cgaggcagat caatctgact cgtatctcca
     8581 ttgacaatca tttttgattg ataaccaaga cgtgtcagga acattttcat ctgagcaact
     8641 gtcgtgttct gtgcttcatc caaaataacg aatgcatcat ccaatgtccg cccccgcatg
     8701 taagctaaag gagcaatttc gatcacgcct cgttccatca atcggttcgt atgatctaat
     8761 ccaaaaattt ggtataaagc atcataaaca ggacgcagat aaggatctac tttttctttt
     8821 agatccccgg gaagaaagcc tagattttcc cctgcttcca cagcaggccg cgtcaaaatg
     8881 attttttgca cttcaccttt tttcaaggca gcgattgcta atactactgc aagaaaagtt
     8941 tttcctgtcc cggctggtcc tacgccaaat acgatatcat gtttggctac cgattcaaca
     9001 tatttctttt ggccgctgtt ttttacccgt ataggttttc cattacggtc tttgacgatc
     9061 tcttcctcat acatctcaac aaaataatct aatgtgcctt tttgccccat tttcagtgcc
     9121 gtcacgacat ctggtgtact tacatggatt cctcgtttga tcaattcctg caatgtccga
     9181 atgactgagg cagccaagga aatctgggat tgttcaccaa tgatctgaat gatttctcca
     9241 cgtgtatgga tggttgtttc tgttgtttct tcaatcagtt ttatatgctt gtcgtgtgca
     9301 cctaaaagca tgctgacatc atcagcagcc gtcaatctaa tctctaaaga actagactct
     9361 tccgtcaaaa atagacatcc cctttttttc gattcattct ttcctatttt accatactca
     9421 gaagaaacta tatagcaaat aagaaaaggt tctaaaagag ttgcttaacc ataagtacca
     9481 agaaacgtta aaaaatagct gcttccattc tctaaaggtc tcgtttgaat aaaaaaagct
     9541 gatcatgtaa gctatgcata agatagagga caagtattcc gtctaactta gcaagtattc
     9601 tctgggtatt tttcttgttt caaaaaaaga aaacaaaata tttgaaatat tcttgcattt
     9661 caaaaaaaac aggtgtatta taacgtcaac aaattaatat acagcaaagc gtcgaggaaa
     9721 acagtaggct attcagcgat ttattcccag tgagtcatgt aagtgagaaa tgacaataaa
     9781 tcgaatagac gaacatcatt tccgagtcaa tgtcgaaaac gtagatcatt gtggctgcct
     9841 ccattatcag gctagcggat aactttatcg aaaagcgtcc gtgaatgaag aagctttttt
     9901 aagcgaaaaa aggtggtacc gttctagaat ctagagccct tttcccaagt ggaaaagggc
     9961 tttttttata ttttaaggtg gtggttttca tggaatctga tattagtggt atttctagtt
    10021 tctgcaacgt ttattttgtt actcaaaatg ataattattg gaggaaaaat ttatggaaga
    10081 aacaaaaact tattcatgga agcagttatt gatggtcagt tcacttatct tcgggatgtt
    10141 cttcgggtca ggtaatctga tctttcctgt ccacttaggt caaatggctg gatcaaactg
    10201 gatcagcgct ggtgtaggat ttgccatctc aggcgctctt ttcccattat tagctatctt
    10261 agctgtagta gtgacaaaaa gtgatggtct gtatgactta gctaaacctg tcggaaaatg
    10321 gtatgctgcc ttgttcttga tccttgttca tttaacgatc ggaccattct ttggtacacc
    10381 gcggacagca gcaactgctt ttgaaatggc tgctaaaccc tttttgcctg aaaaatatga
    10441 ttcattaggt atgtttttat tctctgctct tttcttttta cttgcttatt tccttactgt
    10501 acatccaaca cgtttggtca aatatgtcgg aaagtattta aactccttgt ttcttgcatt
    10561 attagctatc atttttgtcg tttctttatt ccaacccatg gggaatctaa accaatctgc
    10621 tgcagattct tatcagacga acgctttgac aaatggcgtt ttagaaggat acaacacggt
    10681 tgatgcagta gctttacttg ctttaagtgt cacttttgtc catgcagttc gtgatcttgg
    10741 ttttaaagat aaaaaagtca cagaattaac agcaaaagct ggtagcctga gcgttgcgtt
    10801 agaagtcatg atttattttg gtctggtcct tctcggtgcg atgagtttga ataagatgtc
    10861 tttatcagaa aatggcggaa ctgcactttc tcagatcact aatttttatt taggtaactt
    10921 cggtgctagc ttcttagggg taatggtcac attaggtgtc ttcactactg ctatgggact
    10981 cgttgtttct tttgcacaag atttccataa actgttccct aaagtcagtt atatgacatg
    11041 gctgcggtta acgacttttg tctcttttgt cgttgcgaat gccggattgg ataatatcat
    11101 ccaatggtct ttaccagtat tgatgctttt gtatccattg tcacttgcgt taatcttact
    11161 ttccttaaca gcaaaattct tccaaaagag tccatttgtc tatcaagtaa cgatgttctt
    11221 ttctgccgtt ccagccgtat tggatatgtt ggcaaattcg cctgcattag tgagtcagca
    11281 aactgtcgta gcaagcttac taggatttta tcatcattat gttccttttg ctgcattagg
    11341 tcttggctgg gtcgtcccaa cgttgcttgg ttacgctggc agcttgctct tttattatgt
    11401 atatcgcgta tcaggatata aacaagaagc aagtgagtta ccagaagaat aaccaaatca
    11461 aacacaattt aacataagag atattaaaca ggaaaaaaaa cagctagaag aaagaataag
    11521 attctttctt ctagctgttt tttagttctt tttttacgaa ca
//
LOCUS       DAHUVV010000087        11526 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.090, whole genome shotgun sequence.
ACCESSION   DAHUVV010000087 DAHUVV010000000
VERSION     DAHUVV010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.090"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(129..734)
                     /locus_tag="NK767_002218"
     CDS             complement(129..734)
                     /locus_tag="NK767_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCH6695199.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            930..1853
                     /locus_tag="NK767_002219"
     CDS             930..1853
                     /locus_tag="NK767_002219"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCH6695200.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            1912..3477
                     /gene="guaA"
                     /locus_tag="NK767_002220"
     CDS             1912..3477
                     /gene="guaA"
                     /locus_tag="NK767_002220"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCH6695201.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(3968..5599)
                     /locus_tag="NK767_002221"
     CDS             complement(3968..5599)
                     /locus_tag="NK767_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HCH6695202.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(5613..7286)
                     /locus_tag="NK767_002222"
     CDS             complement(5613..7286)
                     /locus_tag="NK767_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCH6695203.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(7299..7616)
                     /locus_tag="NK767_002223"
     CDS             complement(7299..7616)
                     /locus_tag="NK767_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCH6695204.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(7641..9041)
                     /gene="lacG"
                     /locus_tag="NK767_002224"
     CDS             complement(7641..9041)
                     /gene="lacG"
                     /locus_tag="NK767_002224"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCH6695205.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9279..10064
                     /locus_tag="NK767_002225"
     CDS             9279..10064
                     /locus_tag="NK767_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCH6695206.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            10067..10759
                     /locus_tag="NK767_002226"
     CDS             10067..10759
                     /locus_tag="NK767_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCH6695207.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(11033..11404)
                     /locus_tag="NK767_002227"
     CDS             complement(11033..11404)
                     /locus_tag="NK767_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCH6695208.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         3494 a         2474 c         2104 g         3454 t
ORIGIN      
        1 tactatttct ttttgaagaa aaaatagttc tttgttttta aaaacttctc aacaaaaaaa
       61 gaactgatac gtacccgtac atgcacgccc agttccactc tttcgtcgct ccactatttt
      121 tcagctagtt attctttcac acttttgatg atataatatc ctttatcttt tgtgacgatt
      181 tctgcattac caaatgtttc ttccattttc ttttgcgcac tcggcgctcc ttgtttcttc
      241 tggatcacaa ttgtcagtgt accgcccttc ttcaaccgag gatatgcgcc tgtcaaaatt
      301 tcatgtacaa ctttttttcc agcacggatc ggcggattgc ttacgatagc tgcataggtt
      361 tcttcatgaa ctgcttcata gatattcgat tgatggatat caacatgttc gatttggtta
      421 cgctttgcgt ttccttgcgc tagacttacc gctcgttcat taatgtcgac catttcaaca
      481 aaccgctgtg tcgcaaatgc caaagctaac ccaatcggtc catacccgca tcccacatct
      541 aagatctttc cttcatcagg taactcttcc caatcgaaag catcaatcag gacacgtgaa
      601 ccataatcca ctgtttcacg tgaaaataca ccagaatctg ttacaaattg aaaggttctg
      661 ccttttaact caaaagccca ttgttcaaaa tcgtgcgccg tatctgggcg ttcagaatag
      721 taatgatttg tcatgtcttc catccttctc atttctttca tgacaacagt ctatcctttt
      781 atgtatccgt gcgcaagtca agaataagac tccgtttctt tctattataa agattataca
      841 aaaagatcgg caatctctct taagtggtgg ggtttgcgtt tattttgtgt taaaatagtt
      901 ccattgatgg gagaacgcag gaggctaata tgaatgatcc aatgaactac taccgtgtgg
      961 caagagaaga atggcgagaa ttttatcgta atgggcaagc acctttgaca caagatgaac
     1021 tagataatat caaaagtttg aatgaccgca tttccatgca ggatgttcga gatatctatg
     1081 tacctcttgc tcatctgatt tatctttata tgaaagaatt tgaatctttg ttcttgagta
     1141 aaggtttatt tcttcataaa tacgtagaag taccgccttt tattatcggg atcgccggaa
     1201 gtgtagccgt tggaaaaagt acaacagcca gactgctgca gacgattttg tctcgtacct
     1261 tcaaacgaag aaacgttcag ctgatcacga ctgacggatt tctttatcca aatcgtatat
     1321 taaaagagcg tggaatcatg gaccgtaaag gatttcctga aagttatgat atggagatgc
     1381 tgatcgattt cttgaaccgt gtaaaatctg gtcaggaaga gataaaagta cctgtttact
     1441 cacatgatgt gtatgatatc attccagaca agtacgaatt gatccagcag cctgatatct
     1501 tgatcgttga ggggatcaat acgcttcaat tacctgcgaa tcagcaaatc tacgtcagcg
     1561 acttttttga tttttctgtc tatgtcgatg ccgatcccgt gctgatcgaa aaatggtatt
     1621 tggatcgttt tggtgcgtta ctggatacgg ctttccaaaa tccagagaat tattattatc
     1681 gttttgctat cggagatcgt gaagaagcct ttaaaatggc aaaagatgta tggaaaaatg
     1741 tgaatttgaa aaatttacag gaatatatct tgcctacacg aggaagagcc gatataattc
     1801 tccataaaac tgaaaatcat atcatcgatt cgattttcat gcgaaaatat tagcatttta
     1861 aaatagtttg actattttga gataaaattt aaagggatag gggtaataaa tttgacaaac
     1921 gttgccgata tgacgaacgt tgaaaagatt atcgtattag actacggtag tcagtataac
     1981 caattgatca caagacgtat tcgtgatctt ggagtctttt ccgaattatt gagccaccgc
     2041 attactgcag aagaagtaaa agcgatgaat ccaaagggaa tcattctttc tggcggacca
     2101 aacagtgtat atgatgagaa tgcatttggg atcgatcctg agatcttcaa tttaggtatt
     2161 cctgtattag ggatttgcta tggtatgcaa ttgatcacgt acaaacttgg cggttccgta
     2221 gagcctgcta aaaaccgcga atatgggaaa gccttgttag aagtaacaag cgatcaagct
     2281 gtcttgttta aagaaacacc aaaacaacaa acagtatgga tgagccatgg tgacttggta
     2341 actaaagttc ctgaaggctt tgaaacagtc ggaacaagca aagattgtcc gattgcttct
     2401 atccaagaca caacacgtaa tttttatggt atccaattcc atgcagaagt acgtcattcc
     2461 gaatacggaa tcgaattgct gcgccacttt gcctttgatg tttgtcactg tgaaggtaac
     2521 tggacaatgg ataactttat cgatatgcaa gtagaaaaaa tccgtgaaca agttggtgac
     2581 aaaaaagtct tgcttggtct ttcaggtggc gttgattcta gtgtcgttgg ggttctttta
     2641 caaaaagcga tcggtgacca attgacatct atctttgttg accacggtct gctacgcaaa
     2701 ggcgaagcgg aacaagtaat ggaaagtcta ggcggaaaat tcggcttgaa catcatcaaa
     2761 gtcaatgcaa gagatcgctt cttagacaag ttgaaaggtg tatctgaccc agaacaaaaa
     2821 cgtaaaatca ttgggaatga attcgtatat gtttttgatg acgaagcaac aaaacttgct
     2881 ggtgaagaag gcgtctcttt cctagcacaa ggaacgttgt atactgacgt tatcgaatct
     2941 ggtacagaaa cagcacagac gatcaaatct caccataatg taggtggact gccagaagat
     3001 atgcaatttg aattgatcga accattaaac acattattca aagacgaagt ccgtgaatta
     3061 gggactcagt taggcatgcc tgattcaatc gtttggcgtc agccattccc tggtcctggt
     3121 cttggtattc gtgttcttgg cgaaatcact gaagacaagc tagaaatcgt acgcgaatct
     3181 gatgctatct tgcgtgaaga aatcgcagct gctggacttg accgtgatat ctggcaatat
     3241 ttcactgttc ttccagggat ccgctctgtc ggtgttatgg gtgacggacg tacttacgac
     3301 tacacagtcg gtatccgtgc tgtaacttca atcgacggaa tgaccgctga ctttgcccgt
     3361 atcccatggg aagtcttgca aaaaatctct gtacgtatcg taaacgaagt agcccacgtc
     3421 aaccgtatcg tgtatgacat cacttctaaa ccaccagcaa cagtggagtg ggaataaaga
     3481 gccaacataa tatggagggg gaatccctat aaatcaacat tcttcaatag ctaaaatgct
     3541 tgattttcat taaaaataag atggttccct accaaaagag actataatat aagttaattt
     3601 gtttcattct tatcataaaa agaagttctc gaaaaacgaa ctgttttccg agaacttttt
     3661 tctatccatc taagtgctca aatcatattc acgacggaat tctaaggtta ccctttctta
     3721 aacaaacttt atgttatctg cacaactttt tgcaattttt tcattgcttc ttacgaaatc
     3781 gttgaactct ctttagcata atacaaagtg tagatgaagt ctggtgcttt actcgttttg
     3841 gtaaaaatga ctggaaatcg tttctggtta tttttggcaa aggcgaggtg caggttcgat
     3901 tcctgccttc tacgctaacg atgtggctga agggaacctc cgatcttgaa tcgaaacgat
     3961 ccgcagtcta ttgtttcacc aatgaaacaa aatcaacgtt cgtccaagca tttcctgaag
     4021 ccttgctata aaccccaatt tcacaaacac ctgttgtaat tggaatgtct ttgattgtaa
     4081 tttttgtcca ttcacttgtt ttaacaaccg cagtatttag ttccgctcca ccataatttt
     4141 tgatatataa ctgattagta tcaaactctc ccccagacat tacataggca ctcaatgtgt
     4201 aagttccttt gcttatgcca ctaacatttt gataagctga tgcctcgtat gcttgatcct
     4261 gccaaaaagt tagtttgtag tccccaaaaa aagtgcccgc ttccgttttg atcgtaccct
     4321 tagcggttcc ttgttgaaac caggtttgcc aattggaagg actggtcgtt ggtccatcgc
     4381 tttcgaaact tgcattttta actagattta tttgcgcccc tgcttgttta aacgcacgca
     4441 agcttggcaa ggcgtttcca gcaaaatcaa acatcgcttg gttgtcccat tcgtttccca
     4501 tagtggattg aacactgaga taattcatac ctgcttgtgt tgcccaactc acatttccgt
     4561 tgtaccaaag aggttcccaa tagaagaagc ccgccccgcg attattcggt actgcctgaa
     4621 tccgatcggc aagatccagc aaatactctg cttgtcctgt cggtgttggt tcatatcccc
     4681 ccgcagctgc ttcggcttca ccaaaagcat ttggcatagt atcggcattc gctgttgtat
     4741 gcccataagc tgtttcaact acgatcactt ctttaccata ccgttggctg atcgcattca
     4801 tattggtttg aagttcattc attgaaccgt gccaatacgg ataatacgac actccaatta
     4861 aatcaaatga cacacctcga ctcgtgattt catcaaacca ccaagtaaat gctcctgtat
     4921 cgccaccatc agctaagtgg agcattactg gagttgtttt aggttgagtg tctcgaacag
     4981 cttggatacc agacttcaaa aaagcagcca atcgatcaaa ttctcccccg ccttctcccc
     5041 aagacttgcc ataaggccag agcattccgc tattcagttc attgccgatc tgaaccatat
     5101 caggataaac gtccagctgc ttcatttccg acagaacgtt ctttgtatag tcgtacaatg
     5161 ccgtattcat ttcttcaaag gtcagacctt gccatgcttt aggaagattc tgtttcccag
     5221 gatcaaccca aaaatcactg tagtgaaaat ccagcagcac cttaagtccc agattttttg
     5281 ctcgttttgc aaggtcaatc gtcgtttcca gatcatttgt ccctgctcca taactttctc
     5341 cattactatt ataaggatcc tgccacaaac gcagtcgaac atagttgaca ccattatttt
     5401 ttaaaatctc taaggcatct tttgaaatat cgtcttcata atattttgca ccagattttt
     5461 ccatatctga caggatcgaa atatctgctc cgcgaataaa ttcgctggca gatgcttcaa
     5521 cagtttttga aacagccccc aaagccccta acacagctgc aaataaaaca gctttacgca
     5581 accaaattcc cattttcatt atggctcctc ctttattgaa attggttcag aataaactga
     5641 acagcttttt caggatctct cgttaatccg atatattctg ctccttttgt cgcaaccagc
     5701 ttgattccta agcgatcggt gtcttctttg atatcatcat agtaagaatt gacttgcggt
     5761 gctaagacca ccatatcaaa atcctgcagc atttcgtaat gagaaccata agcacccgcc
     5821 attgcactga tcggtgcttt cgattcagat gcaccttttt ttaaagcatt agctaacatc
     5881 gcacttgtcc ctgcgccagc acaaagtacc aacacattga tttctttatt tgcatcgctg
     5941 ataacttgac tcgctgccac aggtacttcc tcacgtacat cggcagaagc accttcctcg
     6001 gttgcgatca cctctgcttt ttgtgcttct ttctctacta agacagcatc gtatgctttg
     6061 caaaatggta gatagatcag gaagtcaacg acgattagaa ccgctgctag aacaaaagct
     6121 aagataccca tgcccgttcc tagaaccaaa ccgatcggtg ctggagttgc ccaaggcaaa
     6181 acgtacataa agctgttcat gcccaataca tcgacaaaaa acttgaacag gcaaacgttt
     6241 acaacaggtg ttaacaaaaa tggaataaaa aaataagggt tcaaaatcat gggtgccgca
     6301 aatagcaatg gttcgttgac agcgaaactt actggaataa aggaagcttt cccaactgcc
     6361 ttcaactgct tagacttagc aaagagtagg aagataaaag gaacaaccaa tgttgcccca
     6421 gttcctccca tagttcctac aaaattccct aaacccaccg tcagtacttt agaagcttgt
     6481 tcaccggaat tgaacaattg cagattggtt tccacattgg catagataat tgccgcaatt
     6541 gccggctcca cgatcgacgg tccatgaaca ccgacaaacc aaaacagcgc catcgctccc
     6601 cagataatag cgattcctaa gtacccgtca gctgctgtaa atagcggctg cagcaccgta
     6661 atgattgctt ctgcaaatga gatttggaag atttgtcgaa tgcctaaatc aattaaaacg
     6721 gccgcaaata ctgaaaaaga gaaaggaaaa atatctcgga aggtttgcga aatcgttcca
     6781 ggaacttctt taggcatttt aatggtaata tctttcagca cacaaaactt atataaatta
     6841 actgttaaaa atgcagcgac aaaggaagcc agtagtccct ttgtccccat atattccgtt
     6901 gaaaatccac cttcaatcgg agagacgctt aacaacagga aacttaccac agaggccagc
     6961 atcacagagg tggtgttgat tactttgttt ttcggtaagc gacgattcat cgaatcagcc
     7021 aagcagcgag cagtggttgc tgaaacgagt aaccctacaa cacccatcga ataattatag
     7081 actttccata gccatgtgga gacagattca ggccattggt aaccaaaaat ctcaggaacc
     7141 gcagcaatca aaatgaaaat gcttgaaaat aaaatagctg gcatcgctgt taaaaatccg
     7201 tcacgaacgg ctcctaaata actattacgt gagatttttt caaaaaatgg tttccctttt
     7261 tcgatttgtt taatgattcc atccatagcg ttcttctcct atccttgatt ctgtctgtac
     7321 agtacaataa agtcttgaat cagatctctc agcaataaag tggtcattaa atgatcctgc
     7381 ccatggataa aaatgaatcc catctccatc tctttcccag atgcttcttc cgctaaaatt
     7441 tttgtttgcg aattatgtgc aagggttaaa ttttcgtcgg cttctttcaa cgccgaatca
     7501 acatgttcaa agtttccttg tcttacttct ttcaataatt tcaataatgt actgcgagcg
     7561 tcgcctgaat atgcaacgat ctcaaaacca agcatttgta attcctcttt attcatcttt
     7621 tcttcctcca ttatcactta ttaaataatc gttttggttt cactgaccaa cttgtaccag
     7681 taagctgatt cttttgggta acgcttctgg gtttcaaaat caacataaaa caagccatac
     7741 cttttgttat acccatttgt ccatgagaaa agatccataa gcgaccacaa aaaatacccc
     7801 ttcacattta ccccggctgt tatcgccttg cttaaggatt ccaaatagac tctcaaatag
     7861 tcgattctcg gctgatccat aataatgcca tcttcaaact gatctttata ccccatcccg
     7921 ttctcagtga tatagatttt gttgtaatgt gggtaatcgc ttttaattcg cagcaacaag
     7981 tcatatagac cttctggata aatcagccag tcccaatcag ttcgaggaat tccttcttta
     8041 taaatacgtt ctccgattcc cttcacttta taaacagagg tccctttttc acccgtaccg
     8101 ttatgatgga ttgcattttc tccatcgtaa gctttgacaa aatggcattg gtagtgattg
     8161 atccctaagt agtcattacg cgtggatgct tttttcaatt caacgaaatc ttcttcagga
     8221 aaatgatagc tggcctgatt tgcttcacat atctcatcaa gagcagtcaa ggtttcagtt
     8281 gaataatagc ctaaataggt agcatctaat aagaaacgga tcgacaatgc atcgtctaaa
     8341 aaggcagcat gcttatcttc cggcgcgtct gttgccgcat atttcgtctc taatgagtga
     8401 actacaccaa tttcacccgg aagttcgttt tctttgaaat agttcactac acgggcatga
     8461 gcaaccatca tattatgaag acaggcgacg atctttgtaa aatcatattt gattcctggt
     8521 gggaaaacac ctaacaaata ttgattggtt gcaactggat agatttcatt aaaggtactc
     8581 cacaccttga cttctttgaa ttcatgaaaa caaaaaatgg cataggaaac aaatgcttct
     8641 attgtttctc gattcaaaaa atctccatgg tcaaacaaac gtttaggggt atcaaaatga
     8701 tgcaacgtca caaacggcgt tacgtttctt ttcttacatt cttcgaacac acgatgataa
     8761 aaagcgattc cttctggatt tggtttccca gctccatcag gaaaaatgcg gctccaagca
     8821 atggataatc ttagaccatt tacaccaaaa cgttcacaca attcaatgtc ctttgcatat
     8881 tgatgataaa aatcacttgc cggatcggga ctgaatcgcc cttgttcctc taaaaagtca
     8941 tcccaagcaa cagcgccttt gccaccttcc ttagtggctc cttcaacctg ataggctgct
     9001 gtggctccgc caaaaataaa atcttctggt aatttaagca tgtacatctc tcctcaaatc
     9061 tataatcagt tttcgctctt tctaactgat tgcattgtaa acgcatccaa aacatttgtc
     9121 aacaaaaaac aaacacaaat aatcataaaa accagaaaac agcaataaaa tctattgtaa
     9181 caaacataaa aaccacccta aatgtttact tttgttcgaa ttagattgac tgttgtccgt
     9241 ttatatgata gattatcctt aaattagagg tgaagacaat gttgaaaaaa gaaagattgc
     9301 ttactatcgt agaaatggtc aataaaaaag gaatccttac cgttaatgaa atcatcaatc
     9361 aattagatgt ttctgatatg accgttcgca gagatttaga tgaattagaa aaatcgggca
     9421 agctgctgcg tgtccatgga ggggctcaaa gtctttccta cactttagat caagaacttt
     9481 ctcacactga aaaagccact ctgcagattc aagaaaaaaa agaaattgct gccacagcag
     9541 cctccttgat tcacgaggga gaaacgattt tcttaggacc aggtacaaca atcgaactat
     9601 tggcgacgca tttactgaac aagaaaattc ggatcattac aaataattat ccagtttttg
     9661 atattcttgc tcaagcagat ggaccagaaa ttctactgat tggcggtgat ttccggaaaa
     9721 ataccggcgc atttgtcgga ccgatcacca acgacaattt aagaagacta aagtttacaa
     9781 aagcatttat cagttcaaat ggcgttcaca acgaagcgat ctctacttac agtaccgaag
     9841 aaggagaagc acagcagatc gctctaaata attcccgtaa gaaatatcta ctcgttgata
     9901 ataaaaaatt caatcgagat gatttttatg tgttttataa tttgcatgat ttcgatctgc
     9961 tcattacgga tcgccagatc tcgcgggagg ttcaagagca ctatgcacaa tatgtcgatc
    10021 tgcaaattgc tgattcaacc gaaaatacca ctataggagc gtaactatga aagggattat
    10081 atttgatttc aacggaacaa tgtttttgga ctctcatctg catgaagctg cttggctgca
    10141 tatgatacat gatcatacaa aagatggttt atcagacgag gatattttaa aaaatattca
    10201 tggtcggacg aatacggaga ttttgaaaca tttcatttca gagcatttga ccgcctctga
    10261 gatcgccgta ttgtcagaag aaaaagaaag ctattatcgt atgctttgct tgcaaaatga
    10321 aaacgagctt cagttgacaa aagggcttga agcgactctt gaccagctga ctgaccaaca
    10381 gattccatta acgattgcca cggctactgt caaagagaat gttgcttttt attttgatat
    10441 tttcgattta gcccgctggt ttgactggga caagattgtc tatgacgacg gctctttccc
    10501 tgggaaaccg caaccggata tttttcttaa agcggcagac aaactgagct tagctccggc
    10561 tgattgtctt gttatcgaag atgcttactc aggattactt gctgccaaac gtgcggggat
    10621 cggaacaatt attgcaattg atccattcgg taaaaacaaa acagtatttg aacaggcaca
    10681 gctaaataaa gatggaacca tcaaagattt tactggtttt tggacagagc acctgtctgc
    10741 cacatccgtt aattcttaaa cgattcaact aactaccaaa gaaaagcaac tgaaatgcac
    10801 acctcctttt ataaaaaact gccagtgaaa cgacatgaaa gatacactgc tatatcctta
    10861 gtgaaatata gcagtgtatc tttgactttt tcattctttt aaccatatga cgcgttcatc
    10921 tctatctata attaacatta aaaatagtgt tttcacatta tttctcaaac caatcttaaa
    10981 aatcatcagg agtacttttt tgatatactt ttattaatgc tgggcgtaat ggttaaaaga
    11041 tttccactag ttcctctcca ccaagctgag ttgaaaaatg cttatctgta aacaacatag
    11101 acaaagagga aataaacgtt tccgtatctt tctctgtccc aaggatatta attaattttc
    11161 ctactaggta agtatcatta aacttaagat ctgccttcac atcaaaagtt ttgataaaag
    11221 cttttatcat gcgtacaatt ttgtctttat cgccataatt gatttcacca ctcatatcaa
    11281 tatagtcact aatccctgaa gaaataatgg caactgcttc ttctttcgcc ttatcagata
    11341 attcatttaa tgactgggat tgattgatga cttcttgagt tttatctgtt acgtaatctc
    11401 tcattatcct atcggcatac tccgcaaagt aatcttgaaa aaattcttct acagggtaac
    11461 caacttcgtt ttcccaacca ggagaaattt cattatcagt acttattaaa tctattaaaa
    11521 accaac
//
LOCUS       DAHUVV010000088        11495 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.091, whole genome shotgun sequence.
ACCESSION   DAHUVV010000088 DAHUVV010000000
VERSION     DAHUVV010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.091"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            3..464
                     /locus_tag="NK767_002228"
     CDS             3..464
                     /locus_tag="NK767_002228"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCH6695209.1"
                     /translation="MLILLVVLVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNE
                     ILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIF
                     QIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            592..1275
                     /locus_tag="NK767_002229"
     CDS             592..1275
                     /locus_tag="NK767_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCH6695210.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1584..1657
                     /locus_tag="NK767_002230"
     tRNA            1584..1657
                     /locus_tag="NK767_002230"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1618..1620,aa:Arg,seq:tct)
     gene            1816..2175
                     /locus_tag="NK767_002231"
     CDS             1816..2175
                     /locus_tag="NK767_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HCH6695211.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2298..3929
                     /locus_tag="NK767_002232"
     CDS             2298..3929
                     /locus_tag="NK767_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HCH6695212.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(3991..4632)
                     /locus_tag="NK767_002233"
     CDS             complement(3991..4632)
                     /locus_tag="NK767_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCH6695213.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4818..4894
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4914..5222
                     /gene="rplU"
                     /locus_tag="NK767_002234"
     CDS             4914..5222
                     /gene="rplU"
                     /locus_tag="NK767_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCH6695214.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5249..5593
                     /locus_tag="NK767_002235"
     CDS             5249..5593
                     /locus_tag="NK767_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCH6695215.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5606..5899
                     /gene="rpmA"
                     /locus_tag="NK767_002236"
     CDS             5606..5899
                     /gene="rpmA"
                     /locus_tag="NK767_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCH6695216.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(5992..7140)
                     /locus_tag="NK767_002237"
     CDS             complement(5992..7140)
                     /locus_tag="NK767_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6695217.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(7152..7463)
                     /locus_tag="NK767_002238"
     CDS             complement(7152..7463)
                     /locus_tag="NK767_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695218.1"
                     /translation="MIYTEFKEWLEKNTTGYETFIVKATNYQIEKNKNRPPKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(7517..7912)
                     /locus_tag="NK767_002239"
     CDS             complement(7517..7912)
                     /locus_tag="NK767_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCH6695219.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(7948..8295)
                     /locus_tag="NK767_002240"
     CDS             complement(7948..8295)
                     /locus_tag="NK767_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6695220.1"
                     /translation="MTTFERVKMLADKRKISIVELEEKLNFSKNSLYAWKKSKPSIDK
                     LNAVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREIL
                     SAMIEAYLEKKDN"
     gene            8593..8814
                     /locus_tag="NK767_002241"
     CDS             8593..8814
                     /locus_tag="NK767_002241"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695221.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPREHQKNSFATG
                     EKKTQKRSEKVLRIIQFLLEKIRHFGNLF"
     gene            8940..9716
                     /locus_tag="NK767_002242"
     CDS             8940..9716
                     /locus_tag="NK767_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCH6695222.1"
                     /translation="MNTPQIFNFGQQEVRTVLLNDEPYFVGKDVAEILGYERADNAVR
                     NHVDEEDRLMHRISASGQNRNMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEER"
     gene            9719..9931
                     /locus_tag="NK767_002243"
     CDS             9719..9931
                     /locus_tag="NK767_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6695223.1"
                     /translation="MSQDFILKVRVALATHNKSQAWLAEKLDISTAYMSDIMNGRRKP
                     DKQIKPIEAVLAELEKEKKHANNNSR"
     gene            9909..10193
                     /locus_tag="NK767_002244"
     CDS             9909..10193
                     /locus_tag="NK767_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695224.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVRKVLGIGDDKLTHWINLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            10214..10402
                     /locus_tag="NK767_002245"
     CDS             10214..10402
                     /locus_tag="NK767_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695225.1"
                     /translation="MSYTLQQEHQILRLIKQRRKQLQDDREALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            10728..11069
                     /locus_tag="NK767_002246"
     CDS             10728..11069
                     /locus_tag="NK767_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695226.1"
                     /translation="MENELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            11062..>11495
                     /locus_tag="NK767_002247"
     CDS             11062..>11495
                     /locus_tag="NK767_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCH6695227.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGDFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIP"
BASE COUNT         3676 a         1895 c         2230 g         3694 t
ORIGIN      
        1 taattcttat tcttctcgtc gtcttggttc cccgaatttt tggtttgact ccttatatta
       61 ttgaggacaa agaaatgagt ccatattatg cacaaggaaa tctgatatat gtacgaccga
      121 aaaatacaaa tgagatttta gtcggagata ttattacata ttatgaaaat tcaggaaaaa
      181 aaatctcgac gcgtcgcgta atagccgttg aaaacaacca cgaagatttt tatacaaaag
      241 ctgatacaaa gacttatatt gagcctggag tagtaaaaaa gagaaatatt atcggtagtc
      301 ccatctttca gattccatat attggactag tcctttcaaa aacggcattt gcatggattc
      361 aggggttatt ctttacgatt gctttttgcc taacaggaat cacagcatgg aatacctttg
      421 tagagttgaa aaagaagcgg agacgaacac agaaattttt gtaagaatgt taaaaacata
      481 aatgtattcc agagatccaa cgactcttct gaacatgaaa cacgagaaaa cactcatgca
      541 gcttgtagat ttagtcagtt gttcgtataa tgtgtgaaga ggaggagtag tatgcgcaat
      601 gaaatgatgc atcgtcctgt agtcaaagaa gaactattgg attttatgcg tacccagcaa
      661 aaaaaattac cgggtgagct aggcaaactg gaagaagaag ctcatgtagc agaagtacca
      721 gttattcccc atgaaacagt tgtatttttg aaatttttac ttggacagct tcagcctgaa
      781 cgaatcttgg agatcggagc agctatcggt ttttcatcaa gtgtcatggc cacgacaatc
      841 ggagaaaacg ctcatgttac gacaattgat cgtttcgatg tcatgatcga gaaagctaaa
      901 aaaaactatg aacgtctagg actgacagat aaagtgactt tgcttgaagg tcaagcagca
      961 gatatcttgc cggagttgtc tggtccgtat gactttattt ttatggatag tgcaaaatca
     1021 aaatatattg aatttttacc tgaatgctta cgtttgttaa gaaaaggtgg cgttttgatg
     1081 gtagatgaca tcttccaagg aggaacgatc ttgctcccag atgaagagat tccccgtggc
     1141 aaacgagcta tccatcgtaa attaaatgaa tttcttcgtg ttgtgatgga tcatcccgat
     1201 ttgacatcga caatcttgcc attaggtgat ggtgtgatct taatgacaaa ggaatcagaa
     1261 acaatcgagt tgtgatacat gattatggca gcctgtagat aatcgattga atcgagggct
     1321 caattcgaac ttgatgtaaa agtttctata tttgtaagtc aattaaagga tacaggaaat
     1381 ctaaataaac aggagtagca tgaagtcaat gtagctcctg ttttcaattg ataaccttag
     1441 aacagctgaa ggagaatgtc tcgttttaaa agattgattt agtagttggg ccaaaataat
     1501 tcattatttg ctaattaaat aactatttcg tattgacaga agcaatttaa ttatctataa
     1561 ttattgatgt tccgctgatt cttgtcatag tagctcagct ggatagagca tccgccttct
     1621 aagcggacgg tcgggggttc gaatccctcc tgtgacgcta aaaaaccgtt agaaactgag
     1681 gtttctaacg gtttttcttt gttttttgtg ccaatattgt ttgttttcaa aggcaaataa
     1741 aataaaaaaa catccttatc gagtacaatt ataaggagca aaaaaataaa actcatcaga
     1801 aaagaggaag aagatatgac gatactatct agaattttag tgactctagt tgcaattgaa
     1861 tttttttata tcatgtatat cgaaacggtg agaactgatt ctgacacgac aagtagggtt
     1921 tttaaaatga gtaaagaaga gttaagcaga aaatctgttc aaacattgtt taagaaccaa
     1981 ggagtgtaca atggactatt aggagtggga ctactttatg gagcttatct ttcttccgca
     2041 tctaaagaaa tcacaagtat gctattaata tctatttttt ttgttgcatt atatggcagt
     2101 ttgactagcg atcgttggat tttactcaaa caaggtgggc tagctctgtt ggcattgctt
     2161 tctattttct tataaatata gaaaaaataa gaatttatct aacaggaaac tttgtgaaaa
     2221 atattacttt atctcatgaa atatgatatt ctaatgaatg gtattttaga aaatttcatg
     2281 aaaaaggagt tagataggtg ttcaaaaatt atgtttcctc actaaaaaaa gaattttcag
     2341 gttatgatct ttcaagattt cagaaagatc ttttggctgg gatcacagta gctgctgtag
     2401 cattgccttt agccttggct tttggggtat caagcggagc tgatgcggcc gcaggtttga
     2461 ttacggctat cattgccggt atcgttattg gcggtttatc aggcggtttt tatcaaatat
     2521 caggtccgac aggtgcgatg gcagctatct tgatgtctat tgcggcaaca aagggaatgc
     2581 aaggtgtatt gcttgctact tttttagcgg gtctattttt acttgcagca ggacttttcc
     2641 gattaggaac actgacttct tttatcccag cacccgtaat tacggggttt acttcaggta
     2701 ttgctatcat tattgcatta ggtcagatcg ataatttatt tggtgttcac tcggaaggta
     2761 caaacgttat tgaaaaacta agtagttatc aaacgatagg atttccgata aacacagcct
     2821 ctttgatcat gggagggcta gtcattttag ggatgtttat ctatccaaaa aaatgggcga
     2881 aacgaatgcc tagttcgctg ctggctatca ttttggcaac agcagttatg ctattgttcc
     2941 atttaccagt agcaactgtg ggtaagattc cacaaacatt gatcagtagt aatcgtttga
     3001 atatgggaga ttttagcttt tctgccttgc aacaagtggc agttccagct attagcattg
     3061 ctttattagg aatgattgaa agcttgctat gtggtgcttc ggctggccga atggcaaata
     3121 agcccttaga tagcaatcaa gaattaatgg cgcaaggaat tggaaatctg ttgcttcctt
     3181 tctttggcgg gattcctgct actgcggcca tcgcccgtac aagcgtggcc atcaaatctg
     3241 gtgcacaaac tagattagct ggtatgatac atgcagttgt cttactgctc tccatgcttg
     3301 tttttgcgcc agtgatgtca cagattccaa tgccagcact tgcaggtgtt ttgattgtga
     3361 cggcttggcg tatgaatgaa tgggaaacga tcaaggagct atttactaat aaatattggt
     3421 cagcagttct attgtttatc cttacgatga tttgtacagt tatctttgat ttaagtattg
     3481 ctatcgtgat cggggtgatc ggcggctgtg tattttttgt tgtcaaaagc gctgctatca
     3541 cgatttcatt agaaacgatc gattggcaaa gaatggggct gccagaaacg gaaaagctga
     3601 ataactggac agtagtgtat attagtggtc cactgttttt tatgtcttca gaaaaactca
     3661 aaaataaatt ggaagaacta aatcatcacg atggtgtcat tttctccatg cgaggcgtac
     3721 caagtattga tttgacggca cgtgcagttt ttgatgaatt tcaagaaaaa gcagagcaaa
     3781 gaggacaaac gattatctat acttccttgc agccagaagt agaaaaacaa ctttctcact
     3841 tgtgggaaca tacattcacg catcaacatc aaacagttgc tcatgcgttg acagctttac
     3901 atgcgaaata tactgcaatt agccattaaa acaatgaaaa agaacagaca agactgtaaa
     3961 ctgaagattt tatggtcttg tctgttcttt ttaatattgc tgtaaaagtg aagctagtac
     4021 ttgccagtct tgatggataa caggtacaag ggagcgattg tagaaaatag ctgctggatg
     4081 aaaaagtgga agaattcgat agtttttttc tgaccattcg taggcatcct tttgttctgt
     4141 taattgtaag acaggccctg tatagagttg accatgattt ttggtaaccg tatattcttt
     4201 gccaagtaaa cgttgaagac caatgtttcc aagtgtcgca attaattttg gctggacctt
     4261 catcagttcg taatccaata ttggtgcatg cgcaaatact tcggaacgag taggtgtccg
     4321 atttggataa acagtttctg actgttgtgt ccgtttattg atccgatgag tcacgcgata
     4381 cgggcggctc cttacagcac tggtgatata cacctcttct cgtgttaagc cagttgaaga
     4441 aagtgcctgc atcaattctt ttccagcttg accgctaaaa ggaacatgcg tatcaatctc
     4501 tgttttccct ggggcttctc ccacgagcat cagtttagga tgaagaggtc cttgcccagc
     4561 gataaatcct tctacaggac ggccgttcat tcttaaagaa gcttcttgta tcagttcttt
     4621 tgggtattcc atttatgctc ctcctttttc ttttattata agaataaaag aaacacaatg
     4681 cgactatttt atttggtaga actgtgagga tagaaaagca gaagtattgc ttgaattttt
     4741 gttttacttt ttgattttaa atttagttga ctaacggttg ctgttctggt aagctagtac
     4801 tgttgtaatt gtggactcca cagctacaac cgctcagaac aagtaataag cacagcatat
     4861 gctgtcactt cggatggcga gtctaagtat atgagaagga ggtgctttat agcatgtacg
     4921 caattattaa aactggtggt aaacaagtaa aagttgaagt gggacaagca atctatgttg
     4981 aaaaacttga cgttgaagca ggagaaaaag ttgtttttga cgaagttatc ttagtaggcg
     5041 gagaatctac aaaagtaggc gctccaactg ttgcaggtgc aactgttgaa ggtactgttg
     5101 aaaaacacgg caaacaaaag aaagtcgtta ctttcaaata caaacctaaa aaacattcac
     5161 accgtaaaca aggtcatcgt caaccatata caaaagtggt tatcgacgcg atcaacgctt
     5221 aatagcgttt gagaaagaag gcctatatat gatcaaagga acatttaagc gaaatgacgc
     5281 aggtcagatc gtttcattca ctttgaccgg gcatgctgat gctggtcctt atggaagtga
     5341 tatcgtttgt gcgggtgttt ctgcgttagc gatcagcact gtcaacggga ttgcctcatt
     5401 agccggtttt gagccgattg ttgaaatgaa tgaagaagaa ggcggttatc tttataccga
     5461 agtcacttca ggaatgacac aagaacaaaa caatattgct gagattctat tagaaaatct
     5521 tttattagga ttacaatcga tagaagctga aaactcagaa tatatccaaa ttaaaacaat
     5581 taatgataaa taggaggtgc agaccatgtt gttatctatg aatctacaat tatttgccca
     5641 caaaaaaggt ggcggttcta catctaacgg ccgtgactca gaatcaaaac gtctaggtgc
     5701 taaacgtgct gatggacaaa cagttacagg tggatcaatt ctttaccgtc aacgcggtac
     5761 aaaaatctat ccaggtgtaa acgtgggtat cggtggagac gacactttat ttgcaaaagt
     5821 tgatggcgta gtacgtttcg aacgtaaagg ccgtaacaaa aaacaagtgt ctgtttatcc
     5881 agtagctcaa gaagcttaat aaataaactg ctctatctct tgtgtggcaa gggatggagc
     5941 tttttttgct aagtaaggga tttttttgac catacttttg accatacttt cttagaagtt
     6001 aatataattt gttagttttt gtgttgtttc ttcttttttg ctgttagtaa tgtgtgtata
     6061 tgtgtccata gtagttttta tatttgcgtg gcccattctt tcttggactt ctttgtgatc
     6121 tgcgcctgcc tcatatagca ttgatgcatg tgtatgtcta aaaccatgca gaccgatata
     6181 aggtaagccg gcacgctcac gatatatatt gtatctttct gccactgact gattaattgt
     6241 gaaattattt tcgtcattgg taaacaaata attagcttta gtaaatccta attgaaataa
     6301 gtactttctt tgttcgagct tccaaaattt tagttgcttt atagtttttt catctaaata
     6361 aattattcga tttgattttt ttgttttagg agtttcagat atataatatt tatctgaacg
     6421 agctacagtt tttttaatgg ccatttcatt tgttttaaaa ttaatattgt cccaagtgag
     6481 tgctaatatt tcgccgattc tacatccgct gaatgctaat agtctaaaga gcgtatagtc
     6541 tctgttttta aaatatggat tctgttcttg agatacttca tttagaaata attccaactg
     6601 ttcttttgta tataatttta atttcttttc tgttttaatt tgtggctttg gaataagtaa
     6661 atttaatgtt ggattatcag atgttaaacc aatatttata gcatacttaa aaactttatt
     6721 catatagttt atgaaaagag ggtactgctt tgaagttcct ttttcactcc aatcatttac
     6781 taccttttga caatatgccg tattaatttt agaaagctta atatttccaa atttgggata
     6841 tatatgttta ttaaagatta ttttagtcct tgaatatgaa ctttctttta cagttttttt
     6901 gtagctttct agccataatt ctgctgcttc tttgaaagta gtatttgtac ttgtgggcat
     6961 tcctgttttt tgtaattcta gttctaacct agaaagtgct gtttttgctt ctttttgtgt
     7021 tctaaatcct cgtttagtag tatatttttt ctttccagtt agcggatcaa taccgagata
     7081 agttttaaag taccatgctt tttcgccatt tttcttttta tattgtttta tcattgccat
     7141 caatattacc cctattcaaa ttctaattct gccatgctat cattgaaaaa ttcgatcagt
     7201 ccttgttcct ctataaattc aagccatatt tccttcccgt taattaatgg aactcctttt
     7261 tctttacgaa ttgtttgata caagttagtc acgacttgtt tccacatttc taatacagct
     7321 ttatctattt tcttatcatc ccagcgtttt tttgggggtc tatttttgtt tttttcaatt
     7381 tgataattag tagctttgac gataaatgtt tcgtatccgg ttgtgttttt ttctaaccat
     7441 tctttgaatt ctgtatatat catttttgtt cacctttcta tttttgaaca tatgttcttt
     7501 ttttgcttaa aaaattttag agagctgcat aacaatagat gcgagctttt aataattctt
     7561 tcacaagtgg ttcgaagcga acgcttaaat tgttatcttc tataaatttc atgtaattga
     7621 tattgtgtgg ttctaattca gactttgcaa tatatctatc taataatttt tcaatcatga
     7681 agcgatcagc ctcatattcc atttttgaat ggagagagaa tgctaagtta tataaaaaat
     7741 aattatcatg atgtttagaa gcgtgtccta attcatgtaa taaggttctt tgtcttctat
     7801 acttagataa attagctttt attactatgg tattcataca agcaacataa tggccatcgg
     7861 catcaaggtt ttcacgctct tctaccttaa cgccgagttc attaattatc atttcaattt
     7921 gactgtccaa atgactcacc tacttattta gttatctttt ttctctaaat atgcttcaat
     7981 catagctgat agtatttccc tatcattatc ggtgagcggt tttccgtcgc tactcataac
     8041 agatgctaaa gcttcttcca cggttagttt tctttcctct ggtgcaactc ccgcgttagg
     8101 atcatctgtg cgtcctagca aataatctgt tgaaacatgg aaatagtcag caactgcatt
     8161 tagtttatca atggatggct tactcttttt ccacgcataa agtgaatttt tactaaaatt
     8221 aagtttttct tccaattcga caatagatat tttgcgttta tctgctaaca tttttactct
     8281 ctcaaatgtt gtcataataa tgattctccg atcatgccta aaacacatct taaaaacttt
     8341 gtagataatc gttgacatct acaaaacttt atagtaatat atgttcgtaa gctaatttaa
     8401 taagctaaca agcaataaga aagataccta ataaagtaaa caaattcaag gtcgggaaac
     8461 ttagaatgtt aatttatagg gtaattctgt gcttatttaa ctatgcactt attctacaaa
     8521 attttgtaga aactgtcaac aaaattagct aattttgtta gcttacatat ttaaagaaaa
     8581 ggaggaaaca tcatgaaaca aaataaaaaa actccttccg caaaaggagc tttaataact
     8641 ttaaatttta aagaccaaaa gtttagtgct gttgttgatg aaattcatcc acgagagcat
     8701 caaaaaaatt cttttgcaac tggcgagaaa aagactcaga aacgctctga gaaagttctt
     8761 cgaataattc aatttctgtt ggaaaagatt cgtcattttg gaaatctatt ttgaattctt
     8821 tattaacagt cttgaatacg gtttgtttga ataaggcgtc gacatccata ttaaattctt
     8881 ccatactatt ttcacctcgc tttctattca aaattatacc aaagaaagga atgagcaaaa
     8941 tgaacacacc acaaattttt aatttcggac aacaggaagt aagaacagtt ttattaaatg
     9001 atgaaccgta ttttgtaggt aaagatgtag ctgagattct tggatacgaa cgtgcagaca
     9061 acgctgtcag aaatcatgtt gacgaagagg ataggctaat gcaccgaatt agtgcatcag
     9121 gtcaaaatcg taatatgaca atcatcaacg agtcaggcct ttacagttta atcttaaaat
     9181 caaaacttcc ttctgccaaa aaattcaaac gatgggtaac aagtgaagtg ttgccagcaa
     9241 ttagaaaaca tggaggttat ctaactccag aaaaagtaga agaagccttg cttaatccag
     9301 atacaatcat ccaattagca actaagttga aagaagaacg tactggaaga ctaatcgcag
     9361 aacaaaaaat tgcagaatac gaacccaaaa tttcgtattt ggatagtatc ttatcttcta
     9421 cagattcagt aacaattagt cagattgcag cagattatgg gatgtctcca caacagatga
     9481 acaaactact tcataaacta ggtattcaga aaaaagtagg caaccaatgg ttattatgca
     9541 agaaacatat gagacaagga tatacaaaat ctcatacaac tgagattccg aaatctgatg
     9601 gtggaacaaa agttgtgatg aatacaaaat ggactcaaaa aggacgtttg ttcatttatg
     9661 agtcgctaaa aaaagaagga tatattccag aaattgatct attagaggag agatagttat
     9721 gtcgcaggac ttcattttaa aagtgcgtgt ggcgctagct acgcataaca agagtcaagc
     9781 ttggttagct gaaaagcttg atatttcaac agcctatatg tcggatatta tgaacgggcg
     9841 tagaaaacca gataaacaaa tcaagccaat tgaggcagta ctagcagaat tagaaaagga
     9901 gaaaaagcat gcaaataaca attccagata atttagtagt ttcagaatta actacacaaa
     9961 ttacgaatgc tgtgcttaat tcattggacg aacgattgca ccttatgaac aaatcagtag
    10021 agcttcctcc atatccaaac aaatcagagg taagaaaagt tttaggaatt ggtgatgaca
    10081 aattaacaca ttggataaac ctaggcttaa aaacacagca gtggagcaag ttagacatca
    10141 gaattgaacg atcggaactc caaagatttt tgaaagaaaa ctttgagttc taaaggcaaa
    10201 ggagaatgat tttatgtcct acacattgca acaagaacat caaattctcc gtttgattaa
    10261 acaacgcagg aaacaattac aagatgatcg tgaagcgctt agaaaagccg atgagctatc
    10321 agatagacaa gctgaactaa tcgcttctga acttgaggat ttgagaatgc tagaaataaa
    10381 aaatagggag attagattat gaagaagaca gacacacttt ttataggatt catcctaggg
    10441 ctattagtga tagtagtaca ccaaagtatt atcgggggaa gtttgttcgc agcattgatg
    10501 gttttaatca atctgcttga ttcaaaagaa aggagcaact atggcacgag aagaagcgct
    10561 aaaaatcggt aaagtgattg ctgataattg gtgggcaaat agccgtccta ttattttaag
    10621 caagcaacat atcgaaaagc aaaaagcatg gcaacaaata aaaaagtgac tccgccgacc
    10681 aaagcaatga gtcactcaaa aaatcataac taaggagatt ttaacatatg gaaaatgaac
    10741 tttccactct agatcaatat ttgactgatc ctagttgggg caaatcgaat atcaaggaaa
    10801 caaataatcg aaaaatcaga cgaaatctct tgacggatga agaactagca tgtgatcaag
    10861 atgacttggg aaattttgtg agtatttggg atcatgtcta tcttatccat ttatcgagga
    10921 agtccaaaaa acctgagtat atctatgtca tcgaagatgg cttgattgat gcgctagaag
    10981 agtacgacag agataacttg attgatatct cttattacgg accaggtaag aaatacattg
    11041 ctgaaatgga ggcagaattt gatgagtgaa atcaaaggga caacgaactt tgaaaaactt
    11101 tttagtcgta agttaaataa aattctcaag aaaaaaggag attttgatta tttatcttgg
    11161 gctcacgcgt gggagattat gaaaaagaat gatccacagg caacggtaac tattaatgag
    11221 tacaaacact acagggttgt ttctggaact catcaagact ttcttgttga ggaatataaa
    11281 ccttttctta tggatgaaac tgggacttat gtatctgtct cagtaacggt taaaggacac
    11341 acggaaaccg aattatttcc tgttttagat tatcgaaacc aaccagttgt taaaccaaat
    11401 gcaatgcaaa tcaataactc attgaagcga tgctttgtga aagcattggc tctacacgga
    11461 ctgggattat atgtatttca aggggaagat attcc
//
LOCUS       DAHUVV010000089        11206 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.092, whole genome shotgun sequence.
ACCESSION   DAHUVV010000089 DAHUVV010000000
VERSION     DAHUVV010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.092"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            6..305
                     /locus_tag="NK767_002248"
     CDS             6..305
                     /locus_tag="NK767_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6695228.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="NK767_002249"
     CDS             378..1739
                     /locus_tag="NK767_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCH6695229.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="NK767_002250"
     CDS             1761..2021
                     /locus_tag="NK767_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695230.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="NK767_002251"
     CDS             2035..2787
                     /locus_tag="NK767_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCH6695231.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="NK767_002252"
     CDS             complement(2881..3486)
                     /locus_tag="NK767_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCH6695232.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="NK767_002253"
     CDS             complement(3539..4513)
                     /locus_tag="NK767_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCH6695233.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="NK767_002254"
     CDS             complement(4891..5610)
                     /locus_tag="NK767_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCH6695234.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="NK767_002255"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="NK767_002255"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCH6695235.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="NK767_002256"
     CDS             7161..8558
                     /locus_tag="NK767_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCH6695236.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="NK767_002257"
     CDS             8639..9079
                     /locus_tag="NK767_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCH6695237.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="NK767_002258"
     CDS             9227..9553
                     /locus_tag="NK767_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCH6695238.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="NK767_002259"
     CDS             9554..10303
                     /locus_tag="NK767_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695239.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="NK767_002260"
     CDS             complement(10383..10838)
                     /locus_tag="NK767_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCH6695240.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3554 a         1960 c         2300 g         3392 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagt
//
LOCUS       DAHUVV010000090        11125 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.093, whole genome shotgun sequence.
ACCESSION   DAHUVV010000090 DAHUVV010000000
VERSION     DAHUVV010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.093"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..1352
                     /locus_tag="NK767_002261"
     CDS             <1..1352
                     /locus_tag="NK767_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695241.1"
                     /translation="KFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAV
                     LYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDI
                     KFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALN
                     NDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPI
                     SLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYA
                     KVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGK
                     YKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGEN
                     KIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1339..2973
                     /locus_tag="NK767_002262"
     CDS             1339..2973
                     /locus_tag="NK767_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695242.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3148..3981
                     /gene="msrA"
                     /locus_tag="NK767_002263"
     CDS             3148..3981
                     /gene="msrA"
                     /locus_tag="NK767_002263"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCH6695243.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4094..5050
                     /locus_tag="NK767_002264"
     CDS             4094..5050
                     /locus_tag="NK767_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCH6695244.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            5339..6037
                     /locus_tag="NK767_002265"
     CDS             5339..6037
                     /locus_tag="NK767_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCH6695245.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            6187..7503
                     /locus_tag="NK767_002266"
     CDS             6187..7503
                     /locus_tag="NK767_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6695246.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            7520..8935
                     /locus_tag="NK767_002267"
     CDS             7520..8935
                     /locus_tag="NK767_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCH6695247.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            9400..9720
                     /locus_tag="NK767_002268"
     CDS             9400..9720
                     /locus_tag="NK767_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6695248.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            9746..10063
                     /locus_tag="NK767_002269"
     CDS             9746..10063
                     /locus_tag="NK767_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCH6695249.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            10092..>11125
                     /locus_tag="NK767_002270"
     CDS             10092..>11125
                     /locus_tag="NK767_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6695250.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLV"
BASE COUNT         3702 a         1765 c         2229 g         3429 t
ORIGIN      
        1 aaaagttttt taaaaagata gatgctgctt ttttatttag ttgtttatat acttttgcaa
       61 cttatcgaac agttgatatc tattatcgat ccgcaattgc agaagcaatc gctcttacga
      121 ttatcccagc tgttttatat tatgcttatg gattgatatt caatagagaa aaaaaagtca
      181 tcgctttatc tgtcagtata agtctacttg tgtatactca tgtcttaact acactattgt
      241 gtagtgcatt gattgcttgt atgattatag ttcgtttgtt tttaagcaag caaataaaaa
      301 acgatataaa atttgtggtc gtgaatttag taaaggctgt ttttttgact tgtgcattaa
      361 ctagctattt ttggtatcct atgttgcagc agatgaatta tcaagaaata cacagtccct
      421 tcaaaacaga tttgcagcta gaagcacttt ctatttcaga tagtatcatt caagcgttaa
      481 ataacgatat tacaacatac actatggggt tattaggatt ggttgcgttg atttttcctt
      541 tattcatact gaaagaaatg gagaaaaaag aaaaaatcat ctatgttggc gtagtttcta
      601 catgggtatg cgtaactaat ttatttccat ggtttttgtt tcaaaaaaca ccgataagct
      661 tactccagtt tccttggcgt atcttaggta tccaaatctt tttttcttca atgatcataa
      721 cgcttgtttt tatgaaaaag actatgaata aaaaatattc atggttaatc attggcgcta
      781 ctgtggtgtt tctactcgtc acgtctatag cagcaaaaga aaattacgca aaagttatcc
      841 aatcaaaaca cggtcatatt gttatcaatg aagaaacact cccacgttat acaacatcag
      901 atatgggagg attatacgat tacgccccaa cagaagcgat aaaagaaagg cctcacttag
      961 aaaaacatga agtaaaagta ggagaatcgt gggaaaaagg gaagtataaa gctgcagata
     1021 ataacattac ttacacagtg gcaagcaaca aaaagcaaaa agtgacagta ccagtatatg
     1081 cttatttagg tacacaggta aaagtaaacg gtaaagtcgt aaatgactct tataaaaaac
     1141 atggcttagt gaatgtggat gtaaatgctg gagaaaataa gatagaaatt acaacaaaat
     1201 atactccaac tgctctcatg gctttatgga tcagtattct gacttattgt ttagtggtgt
     1261 attcaagttt taaacgattt gcacctaata agttgaaaga ttctcgatat gtgactaaaa
     1321 aggagataaa caagaagaat gaaaataaat aaaaaagtag taatattgag tggattaatt
     1381 atagttagta gtatatattc tttcatcttt ttcaaaaata cacagagtat ttataccact
     1441 ggagatcttt cttttcactt atctcgaatc aaggggttat cgactatttt taccagtccg
     1501 attaattatg agacattcaa ttatactgga tatggtgtaa attattttta tccctttctg
     1561 actttttttc cagctgtaat gttatattgg atgacaaaaa atttgattgt aagctatatt
     1621 atttacgttt ggttattgaa cttgtgtaca acactcgtag cttatcatta tggcgagcgg
     1681 tttttaaaac aaaaaaaagc ggcattttta tttagttgtc tctatatttt ctctgcttat
     1741 cggacagtag acatctacta tcgttccgca attgccgaag ctatcgcaat aacacttgtt
     1801 atccctgtat tattttatgc ttatcaaatc atctctggaa aagaagaaaa gtatccgtct
     1861 gtaaagctag cattgagtat gagtttgcta gtgtacagcc atgtactatc aacattaatg
     1921 agtacagcat taattatcat ttttattttt attcgtttgg tttctaaagg tttcaagaat
     1981 gcagatttta ttgctatatt taaaaaatta tttagtgcgg caggaatgac attggttctg
     2041 acgagtgctt tttggtatcc gatgtttgaa caaatgctct accaaaaaat caacaaacct
     2101 agcgtgacaa atttatatgc tcatgcatct aatgtgttcg atagtctgac tgaagctatg
     2161 aacaacgact taactactta ctcaatggga ctagttggtc tgttatcttt atgtattccc
     2221 ctgattttat ttaaaaaatt aacaaacata gaaaaaaaga tttattatgg tacgtgtttg
     2281 acttggctag ctacaactag tttggtgcca tggtatcttc tgcaaaatac gccagccaaa
     2341 ttgttacaat ttccttggcg gatcttaagc ttgcaaataa tattctcttc tctgatatta
     2401 acgatgatat ttttcaaaaa tagaagatac aataagacaa gagaactatt ttatttattt
     2461 atgactgttt tgttattaat cactttaact gtttctagta aaaaaaattt caatcaaaaa
     2521 ataatttctc agccgggaca tattgttgta aacaaagaaa cagtagaggc gtataccact
     2581 aaaggaatgg attatttttt atatgattat tctcctatcg cttcattgga gaataaggag
     2641 catataataa aacacgaagc aaagattggc agtcaatggg taaaccttcc ttataaaaca
     2701 actggtagtt ctatcatata tagagtaact aatgagaaga gccaagtaat cacacttcct
     2761 gtatatgctt atttaggaac gaatgtagaa ataaatcatc aacctgtaaa ggagcaggtg
     2821 aatgctaatg gattggtaca aataagcgga gtcaaaggag ttgctgatat taaagtaagc
     2881 acaagttata cagtaacgac tattatttct tatcttgtta cattaactgc atatttatgt
     2941 ttatttttta aaatagtttt ccataaaagg taaattaact tttcatttat aaaataaatt
     3001 gattaaaaag gagaatctgt cgataaggat tctctctttt ttatacgaaa aatctgtagg
     3061 gggatgaagt aatcgcttat ttcctcactt tatgataaat tacagctaaa gtcactagct
     3121 tgtcttcttc ggaaaggagg agaatctatg gatgaggaac aaatattcat tcaaacactt
     3181 tataatctga ttttgaatcc gaatacacga gattgggaac gaaaagtatt gattcagaca
     3241 aagaatgaca taagagaaaa tatatccgtt aaagaacagt tatcaaaact agaagcaaca
     3301 ctacgtccat tagctatccg aatgaatctg acgcctgatg taatggactt ttatttattg
     3361 attacggaag gatttgataa ggagcaaaaa tatgactttt ccaagcatgc catgcaagat
     3421 gctgactatc aagaacgtgc agtatttgca gggggatgtt tctggtgtat ggtcgagccg
     3481 tttgaatcga aaaaagggat cctatctgtt ttgtcaggat acacgggcgg acatgttgaa
     3541 aaaccaaatt atgatcaagt aagtggtggt tacacaggtc atgtggaagc tgtggagatc
     3601 atttatgaca caagagaaat aagttatagc gagttgctga caatctattg gcaaattact
     3661 gatcccacag atacatttgg acaatttcaa gatagaggga agcaatatcg tccagtgatt
     3721 ttttatcaag tcgaacgaca aaaagaatta gcggaacaat caaaacagaa actagacagt
     3781 tcaggaactt tccaccaacc aatcgtcaca aaaatcgaac cagccggtac gttttggcca
     3841 gcagaaaact atcatcagca attttacaaa aaacaaccga aacgttataa aaaaatccag
     3901 caagcacgta atcaatttct gatctatcaa cgagtaaaaa acaaatggca aaaaaatata
     3961 cgaaaaaatc attttgatta actccttttc tttaaaatgt agtaaactaa aaatagaaaa
     4021 aagaaagcgt tttaaaaaga tttgatgagg aggagaaaaa ttgtatattg cagatagtgc
     4081 acgttatgaa aaaatggaat atcgtaaatc cggtcattca ggtttgaaat taccgatttt
     4141 atctctcgga ttatggcaaa attttggtga ttatgatcct atccataatc aaagagagat
     4201 tttaagaggc gcattcgaca tgggaattac ccatttcgac cttgcgaata attatggagg
     4261 ccctgctgga gctgcagaga aaaactttgg gcgtattttt agagaagatt ttcaagccta
     4321 ccgtgatgaa ctgattattt ctagcaaagc aggttatcat atgtgggaag ggccatatgg
     4381 ggaatggggc tctcgtaaat ccatcatttc aagttgtgac caaagtctcc aaagaatggg
     4441 gctagattat gttgacatct tctatcatca tcgcccagac cctgatacac ctttggaaga
     4501 aacagcagaa gctttgatgc aacttgtaag acaaggaaaa gcattatata ttggtatatc
     4561 caactataat ggagaagata caaaaaaaat gactgaaatt ttaaaaagaa aggaagcacc
     4621 gttcattatt catcagatgc gctacaatat gttttctcgt gcactactag aagacgatct
     4681 atctccagta ttagaagaag aagggctagg ggcaatcacg ttcagtccgc ttgctcaagg
     4741 gttgctgaca aatcgttatc tacatggtat tcctgaagat tctagagcac atcgtaagga
     4801 aataccattc ctttcagaag aacaagttgg atcaactctg gaaaaaatca aagcactgca
     4861 aacaatcgcc gtatcacgag gacaatcatt agcgcaaatg gcccttgcat ggaatttgcg
     4921 acaaaaatct gttacaagtg tactggtcgg agcaagtcgg ttaagtcagt tacaagaaag
     4981 tgttcgaatg atggataatt tagatttctc accagaagaa gaattgagaa tcgaccaaat
     5041 tctagaatag aaacgatgta atcaaacttt ttatccatat attaaaaaaa acaaaagatg
     5101 acatcaagaa tcgaatgaag atcgtctgtt attgaacatc atattttttg tcgttcaaaa
     5161 taaaaatgat cgaaggctag gagtgaattt tatcctagtc ttttttatgg taaatatctt
     5221 cttcgtttgt atgtctatga acggattaat caattttgta tacaaaaaat catcagatga
     5281 ttcaaacgtg ttataataat tacgaaaaaa tacatcagat gaaaaggagg tatgtggttt
     5341 ggaagaaaat aaaaccctag tagaagtaac tactgacaag atccttagat atttaaagga
     5401 aatgcagttg caagtaggag ataaactgcc gaatgaatat gaattgtccg attatttaga
     5461 ggtggggcga agcacggtgc gagaagctgt gcgggctcta gcttccagga atatccttga
     5521 agttcggcaa ggagcgggga catttgtcag tgataagcga gggattgcca atgacccact
     5581 cggtttttct ttgattgaag atcaggatcg gatgatacga gacttatttg aactgagata
     5641 tttattggag cctaagatgg caggtttggc agcggaatat gcaacgaaga atcaaatcgt
     5701 ccaaatgaaa aaattaacgc aaaagattga aacttctttt cgagaaggca cacaagaaca
     5761 tgttcaatta gacatcgatt ttcatacgat gattgcagaa gctagcggaa acgtagcctt
     5821 gctgcatatt atcccaatca ttaatgaatc aattgctttg tttaataaaa attatgatat
     5881 ccagcatctg aaaatcgaga cgatcgatat tcatacagag atcgttcagg ccatcgaaag
     5941 gcggaatgca cttgctgcgt ttgatgcaat gactgtccat atggcaataa atcggagaga
     6001 attacagaaa ataataggag aaaaagaaac taattgacag ttcatctgat gtattttatg
     6061 ataaatacat cagatgaatt tttgttttat atttggtaaa aaagaggagt aaatagtttc
     6121 tgttcatcga aatgtaagcg ctgactaata gacaaaaaga gatgacgtca aggagaagaa
     6181 gcaggaatga ggggatctgg aatgagtttt atggataaat tagccaatgt tttagggaac
     6241 gtggcaacga gaatcaataa tttaaggtat atcatggtga taaaaaacgc atttgctgca
     6301 ttgattccgg taattattac gggtgctttt ggcacattgt tttcagcaat ggtctttgac
     6361 tcggaaaatg gcttagcaca gataagtttc ttgcgttttt tagaagaatt aaagcctatc
     6421 gcatctgcca tcagctatgt cacactaagt ttcttgacca tctatgctgt ttttctgata
     6481 gggatcgaat tagccaagct gaataaagtg gatggtgtat tccctggtat catcgcagtg
     6541 atgtcttatt tatctgttaa tccaactgtt tatgaattag tgaatgaaaa tcagactgtt
     6601 atagtagaaa acgtattggc taaacaatat acagatacga aagggttatt tttgggaatg
     6661 ttggtctcca tcctttctat tgaactttat tgctggttga ggaaacagaa gaaactacag
     6721 ctgagaatgc ctgataccgt gccaacgaat gtttctgcta gtttttctgc tttattcccg
     6781 acgattctga ctgtatcggg tatcgcggct gcgggattta tcatcaaaga acttacagga
     6841 atgtaccttt atgatatcat ttacaatgtc gtacaacgcc cattagaagg tgtcgtacaa
     6901 gggttaccgg gtattttgct attgatgttt attgcgcaag tcttttgggt gattggtatc
     6961 catggaaatc aaatggtgaa gccgatacgg gaaccgttgc ttttagcggc aattgctgta
     7021 aatacggaag cttttgaagc tggaaaagaa gtaccgaaca ttattactat gccattttgg
     7081 gatatgtata tgagtatggg cgggtctgga gtcacgatcg gtttactgat tgcagtattg
     7141 ttagttggaa aacgggaaga tatgaaacaa atcaccaagt tatcccttgc tcctgggatc
     7201 ttcaacatca atgaaccagt tatttttggt atgccgatca tgctgaatcc aattttagct
     7261 attccgttta tcttaacgcc tcttatcact ggaacgattg gttattttgc aaccgcttta
     7321 ggatttgcgg cgaaagcagt tgtaatggtc ccttggccaa tgccgccgat cgtcaatgct
     7381 tatctggcaa cagcaggaaa tatcggtgca gttattacgc aacttgtttg tattattgtt
     7441 tctattttga tctatctgcc atttgtaaaa gtagcaaatc gtgtggaaaa tgatgggatc
     7501 taaaaaacag gaggaaatca tgaaaattac tattccaaaa aattttattc ttggtgctgc
     7561 atcttctgct tggcaaacgg aaggttggaa aggaaaaaaa gaaggacagg attcgtttat
     7621 agatagctgg tataaaaatg aacattttgt ttggcataat gggtatggcc cagccgtagc
     7681 aactaatttt atggaacaat accagcagga tgtggatctg atgaaatcca tccatctgac
     7741 acattatcgg acttctatca attgggctcg tttttttact gattatgaga actgtatagt
     7801 agatgaagaa tatgctcagc atataagtga aatgatcgac tctttgatcg aagcagacgt
     7861 agaaccgatg atttgtttgg aacattatga agtcccagct tttcttttgg aaaaatatga
     7921 tggctggtct tcaagaaaag tagttgattt ttatgctaag tatgctcaaa ttgcctttga
     7981 tcgttacggt gatagggtaa aaaactggtt tacattcaac gaaccaatcg ttccccaaac
     8041 gcgttgctat ttggatgctg ttcgctggcc gcatgaacaa aacacaaaaa agtggatgat
     8101 gtggaattat cataaagttt tagcaacggc taaagcagta gagatttacc gcagcggaaa
     8161 ttatggtgga aggattggct gtattttaaa tcctgaaatg gtctatgctc gatccaattc
     8221 tccagaagac caaaaagctg cttatatgta tgatttgttt tataatcgag tattttttga
     8281 tccgatggta aaaggatact acacagaaga gttcctggag ttgtgtcaaa aacaatcgat
     8341 ttatttcgat cctgcaacag aggatttaga aacgattcag catcatacag tagatttcct
     8401 tggaatcaat caatattatc ctaaacgcgt tcgcgcccct cgttatcaat ggaatgaaga
     8461 gacacctttc catcctgaac gatattatga agtctttgat ttacctggga agaaaatgaa
     8521 taattccagg ggatgggaaa tctatccgaa aatcatgtat gatatagcca tgtatctaaa
     8581 agaaaattat cataatatcc cttggctgat aacagaaaac ggtatgggaa gagaatatga
     8641 agaacagtat atggatgaga aaggaatcgt gcaagatgat taccgtatcg aatttatcgg
     8701 acagcatttg cattacttga tcaaagcaac agaagaaggc gcaaattgcg aaggctatat
     8761 gttatgggca ttcactgact gtgtgtcacc aatgaatgca tttaagaatc gatatggatt
     8821 agttcgaatc gaattgaacg atgaacgaag cagaacattg aaaaaatctg cttcgtggta
     8881 tcagactttg attgataaga gagaattgga tttgcctgac gaaatctata aataaataga
     8941 agttttttgt agatgttcat tgtatttact ggttatttga aataaaagag gttgtgaatc
     9001 cagcgttttg agctaatacc cagggtcagc tgattgaaca ccgtttattg agaggttgtg
     9061 aaaaagctgt cctgcatcaa gtaataagaa tagcaaagta aaaacaacat cttatgtttt
     9121 taacttggtt ggacttatta ccgcagatgc aagcttttga ataccgttta tttgaaaaaa
     9181 gttgtgacaa gttttgtgtc acaacttttt ttgtgcacgt taaaagaaaa aaggtaataa
     9241 aaattaattt gtgtgtttag tgtgcgcttt ctttttttag tgtgtatgca acttggtata
     9301 gaccttaaat gtgatgatat caacttttta aagcgctttt aaagttggca cgctacttgc
     9361 ttatatacaa gtgtaagtat agaaaggaac gtgtttaaaa tggaaaagaa aacaatcatg
     9421 cttgtatgtt cagctggaat gagtacaagt ctcctagtca caaaaatgca aaaggcagca
     9481 gaagcaaaag gaatggaagc agacatcttt gctgtatctg cttcagatgc ggataatcaa
     9541 ctagaatcta aaaatgtaga cgttttactt ttaggaccgc aagtacgctt tatgcaaaat
     9601 caatttgctg aaaagctagc acctaaaggc attcctttag atgtaatcaa tatggctgac
     9661 tatggcatga tgaatggtga aaaagtactc gaccaagcag aaaaactaat aaacaaataa
     9721 taaaaattat cggaggatac aataaatgga agatcaaaaa aatctagaag ccatcatggg
     9781 tctgatcatg tttggcggaa atgcaaaaag cgatgcaatg gaagcgattg ctgctgcgaa
     9841 aaaaggtgat ttcactttag cagatgaaaa aatcaaagat gcagaggaat ctttagtcca
     9901 agcacatcac tctcaaacag agatgctgac acaagaagca caaggaaatc acgttgaagt
     9961 cacgcttttg acggtccaca gtcaagatca tctgatgaca tctatagctt ttacagattt
    10021 agcaaaagag atcattgatc tgtatcgtcg attgaacgcg taatctacag taaagaaaag
    10081 agggaagatt tatggataaa tttgttgcat ttatggagaa acattttata cctgtagcat
    10141 caaaaattgg tgcacaacgg catttagttg cgattcggga ctcatttatg gtgagtatgc
    10201 cgttaatgat cttaggagca ttagcagtaa tgatcaataa cttaccgatt cccgggttcc
    10261 aagaattgat gaactcaatt tttggtggtg aatcatggaa aggattcggg gctgctgctt
    10321 ggaacggtac atttgccatt ctttctgtct tgattgcttt cttgcttgct tatcatttag
    10381 ctaacggcta ccgaaaagac ggtgtttccg caggtgtgat ttctttaggc tctttcttcg
    10441 ctttaggcgg agcattaggt atgagctcaa acggtttgtt tattgctatt attgttggga
    10501 ttatttcaac agaaatcttt gtacgtttaa gcggaaatga aaaattgatc atcaaaatgc
    10561 ctgatggtgt gccaccggca gttgcaaaag catttgcttc attactacca gcaatgatca
    10621 ctatctctgc gtttgcttta gttgctgcaa tttttgctgc attcggtgtt gatgatatcg
    10681 taggttcttt ctatacagtg gtccaagaac catttatggg acttgcaaac tcttatccat
    10741 ctgccttact gcttgcattt atcacaccat tcttatggtt ctttggttta cacggagcga
    10801 atatggtcga tcctttgatg caaacaatca atgcgccagc cattgaagca aacgttaatg
    10861 caatcgcagc tggacatagc gcaccgttta tcgtgaacaa acctttcttt gacagctttg
    10921 tcaacttagg aggaacaggt gcaactttag gtttactgtt agctatttat ttagtaggac
    10981 gtaaaaacaa accatatatg gttgtaacaa acttgtcgat tgcgccaggt gtcttcaata
    11041 ttaatgaacc aacgatgttt gggttaccta tcgtgttgaa cccgatcatg ttcattccat
    11101 ttattttgac accaatggtc ttagt
//
LOCUS       DAHUVV010000091        10809 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.094, whole genome shotgun sequence.
ACCESSION   DAHUVV010000091 DAHUVV010000000
VERSION     DAHUVV010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.094"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            90..1349
                     /gene="lysA"
                     /locus_tag="NK767_002271"
     CDS             90..1349
                     /gene="lysA"
                     /locus_tag="NK767_002271"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCH6695251.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1336..2136
                     /gene="dapF"
                     /locus_tag="NK767_002272"
     CDS             1336..2136
                     /gene="dapF"
                     /locus_tag="NK767_002272"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCH6695252.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2139..3020
                     /locus_tag="NK767_002273"
     CDS             2139..3020
                     /locus_tag="NK767_002273"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCH6695253.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3017..3733
                     /locus_tag="NK767_002274"
     CDS             3017..3733
                     /locus_tag="NK767_002274"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCH6695254.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3739..4434
                     /gene="dapD"
                     /locus_tag="NK767_002275"
     CDS             3739..4434
                     /gene="dapD"
                     /locus_tag="NK767_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCH6695255.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4450..5451
                     /locus_tag="NK767_002276"
     CDS             4450..5451
                     /locus_tag="NK767_002276"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCH6695256.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5448..6809
                     /locus_tag="NK767_002277"
     CDS             5448..6809
                     /locus_tag="NK767_002277"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCH6695257.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6769..7920
                     /locus_tag="NK767_002278"
     CDS             6769..7920
                     /locus_tag="NK767_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCH6695258.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7895..9064
                     /locus_tag="NK767_002279"
     CDS             7895..9064
                     /locus_tag="NK767_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCH6695259.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9239..10618
                     /locus_tag="NK767_002280"
     CDS             9239..10618
                     /locus_tag="NK767_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCH6695260.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3585 a         1896 c         2426 g         2902 t
ORIGIN      
        1 tttgtttaat attattccaa taaaattcaa atctttatca aatgaaattc caataaaaac
       61 ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag
      121 caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga
      181 gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc
      241 tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat
      301 tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta
      361 gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg
      421 aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta ttagcagaat
      481 taagccaacg ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag
      541 cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg
      601 aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag
      661 aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa
      721 tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg
      781 gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa
      841 aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat
      901 tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg
      961 gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg atgacagata
     1021 atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg
     1081 aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa
     1141 ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg
     1201 gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt
     1261 ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa
     1321 ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta
     1381 ttagattacg cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa
     1441 aaatatgata tagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac
     1501 aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac
     1561 gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca
     1621 agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca
     1681 gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata
     1741 gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat tcataccgtt
     1801 gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat
     1861 catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa
     1921 ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc
     1981 tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt
     2041 cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca
     2101 gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg
     2161 cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga
     2221 tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat
     2281 cttctacgct cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg
     2341 ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa
     2401 aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca
     2461 acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga
     2521 ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat
     2581 tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg
     2641 cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg
     2701 atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga
     2761 atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc
     2821 aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca
     2881 atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc
     2941 tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag
     3001 aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt
     3061 cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga
     3121 agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga
     3181 taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac
     3241 gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt
     3301 gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc
     3361 agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa
     3421 agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct
     3481 gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag
     3541 tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat
     3601 atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc
     3661 agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga
     3721 caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct
     3781 aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt
     3841 aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg
     3901 gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac
     3961 agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt
     4021 atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat
     4081 attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga
     4141 gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa
     4201 ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg
     4261 attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta
     4321 gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt
     4381 gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga
     4441 tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa
     4501 acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct
     4561 tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa
     4621 ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta
     4681 tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca
     4741 gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat
     4801 acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt
     4861 aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt
     4921 tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgaggaacca
     4981 gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa
     5041 aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag
     5101 cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc
     5161 tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc
     5221 cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt
     5281 cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag
     5341 aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc
     5401 gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc
     5461 tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc
     5521 gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac
     5581 gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc
     5641 atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag
     5701 atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg
     5761 aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac
     5821 tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact
     5881 atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat
     5941 atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat
     6001 tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa
     6061 gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc
     6121 atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct tatatggggg
     6181 ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa
     6241 taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa
     6301 aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc actttattca
     6361 aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac
     6421 atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag
     6481 cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg
     6541 tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata
     6601 aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca
     6661 catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc
     6721 aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa
     6781 tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa
     6841 tatttatatg aacaagtaaa aaacagcgag gccgttatcc atgaaatcgg cgaaacagga
     6901 ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat
     6961 gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg
     7021 catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact
     7081 aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt
     7141 tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca
     7201 atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga
     7261 cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt
     7321 gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt
     7381 tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc
     7441 caaggtggaa cgatccgaaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt
     7501 tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag
     7561 tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac
     7621 catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca
     7681 gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt
     7741 ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt
     7801 ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa
     7861 atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag
     7921 tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc
     7981 gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt
     8041 caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg
     8101 attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta
     8161 tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt
     8221 gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata
     8281 tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg
     8341 gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa
     8401 agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt
     8461 gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata
     8521 tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac
     8581 aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat
     8641 ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc
     8701 cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag
     8761 cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc
     8821 tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc
     8881 tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc
     8941 gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc
     9001 tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca
     9061 gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag
     9121 acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta
     9181 atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat
     9241 ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga
     9301 actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat
     9361 ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga
     9421 tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca
     9481 cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa
     9541 cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa
     9601 tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc
     9661 aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc
     9721 gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt
     9781 gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt
     9841 agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt
     9901 tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct
     9961 agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt
    10021 agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta
    10081 tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc
    10141 tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt
    10201 accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca
    10261 ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt
    10321 gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa
    10381 agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt
    10441 atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact
    10501 gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg
    10561 gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga
    10621 aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag
    10681 ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag
    10741 cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc
    10801 aaaagttag
//
LOCUS       DAHUVV010000092        10574 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.095, whole genome shotgun sequence.
ACCESSION   DAHUVV010000092 DAHUVV010000000
VERSION     DAHUVV010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.095"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            12..84
                     /locus_tag="NK767_002281"
     tRNA            12..84
                     /locus_tag="NK767_002281"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="NK767_002282"
     tRNA            88..160
                     /locus_tag="NK767_002282"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="NK767_002283"
     tRNA            168..249
                     /locus_tag="NK767_002283"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="NK767_002284"
     tRNA            267..339
                     /locus_tag="NK767_002284"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="NK767_002285"
     tRNA            351..422
                     /locus_tag="NK767_002285"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="NK767_002286"
     tRNA            439..524
                     /locus_tag="NK767_002286"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="NK767_002287"
     tRNA            535..608
                     /locus_tag="NK767_002287"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="NK767_002288"
     tRNA            618..691
                     /locus_tag="NK767_002288"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="NK767_002289"
     tRNA            711..783
                     /locus_tag="NK767_002289"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="NK767_002290"
     tRNA            811..884
                     /locus_tag="NK767_002290"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="NK767_002291"
     tRNA            898..971
                     /locus_tag="NK767_002291"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="NK767_002292"
     tRNA            995..1084
                     /locus_tag="NK767_002292"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="NK767_002293"
     tRNA            1097..1170
                     /locus_tag="NK767_002293"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="NK767_002294"
     tRNA            1189..1264
                     /locus_tag="NK767_002294"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="NK767_002295"
     tRNA            1266..1336
                     /locus_tag="NK767_002295"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="NK767_002296"
     tRNA            1350..1423
                     /locus_tag="NK767_002296"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="NK767_002297"
     tRNA            1448..1535
                     /locus_tag="NK767_002297"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="NK767_002298"
     tRNA            1557..1628
                     /locus_tag="NK767_002298"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="NK767_002299"
     tRNA            1651..1740
                     /locus_tag="NK767_002299"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="NK767_002300"
     tRNA            1753..1826
                     /locus_tag="NK767_002300"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="NK767_002301"
     tRNA            1845..1917
                     /locus_tag="NK767_002301"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="NK767_002302"
     tRNA            1921..1993
                     /locus_tag="NK767_002302"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="NK767_002303"
     tRNA            1998..2068
                     /locus_tag="NK767_002303"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="NK767_002304"
     tRNA            2081..2154
                     /locus_tag="NK767_002304"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="NK767_002305"
     tRNA            2166..2237
                     /locus_tag="NK767_002305"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="NK767_002306"
     CDS             2503..3696
                     /locus_tag="NK767_002306"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCH6695261.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="NK767_002307"
     CDS             3826..5298
                     /locus_tag="NK767_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6695262.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="NK767_002308"
     CDS             5381..6169
                     /locus_tag="NK767_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCH6695263.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="NK767_002309"
     CDS             complement(6236..6904)
                     /locus_tag="NK767_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCH6695264.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="NK767_002310"
     CDS             7019..8731
                     /locus_tag="NK767_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6695265.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="NK767_002311"
     CDS             8734..10506
                     /locus_tag="NK767_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6695266.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         3033 a         1926 c         2453 g         3162 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggca
//
LOCUS       DAHUVV010000093        10247 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.096, whole genome shotgun sequence.
ACCESSION   DAHUVV010000093 DAHUVV010000000
VERSION     DAHUVV010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.096"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            278..787
                     /locus_tag="NK767_002312"
     CDS             278..787
                     /locus_tag="NK767_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCH6695267.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="NK767_002313"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="NK767_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCH6695268.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="NK767_002314"
     CDS             2322..3302
                     /locus_tag="NK767_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6695269.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="NK767_002315"
     CDS             3424..4842
                     /locus_tag="NK767_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HCH6695270.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="NK767_002316"
     CDS             4913..6382
                     /locus_tag="NK767_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCH6695271.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="NK767_002317"
     CDS             6392..6859
                     /locus_tag="NK767_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCH6695272.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="NK767_002318"
     CDS             6895..7767
                     /locus_tag="NK767_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCH6695273.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..>10247
                     /locus_tag="NK767_002319"
     CDS             7849..>10247
                     /locus_tag="NK767_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCH6695274.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     K"
BASE COUNT         3562 a         1478 c         2032 g         3175 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaaga
//
LOCUS       DAHUVV010000094        10208 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.097, whole genome shotgun sequence.
ACCESSION   DAHUVV010000094 DAHUVV010000000
VERSION     DAHUVV010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.097"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(122..1888)
                     /locus_tag="NK767_002320"
     CDS             complement(122..1888)
                     /locus_tag="NK767_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6695275.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1848..3602)
                     /locus_tag="NK767_002321"
     CDS             complement(1848..3602)
                     /locus_tag="NK767_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6695276.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3712..4281)
                     /locus_tag="NK767_002322"
     CDS             complement(3712..4281)
                     /locus_tag="NK767_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HCH6695277.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4299..5714)
                     /locus_tag="NK767_002323"
     CDS             complement(4299..5714)
                     /locus_tag="NK767_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCH6695278.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5725..6393)
                     /locus_tag="NK767_002324"
     CDS             complement(5725..6393)
                     /locus_tag="NK767_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCH6695279.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6523..7107)
                     /locus_tag="NK767_002325"
     CDS             complement(6523..7107)
                     /locus_tag="NK767_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCH6695280.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7438..7671)
                     /locus_tag="NK767_002326"
     CDS             complement(7438..7671)
                     /locus_tag="NK767_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HCH6695281.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7686..8636)
                     /locus_tag="NK767_002327"
     CDS             complement(7686..8636)
                     /locus_tag="NK767_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCH6695282.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8633..9475)
                     /locus_tag="NK767_002328"
     CDS             complement(8633..9475)
                     /locus_tag="NK767_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCH6695283.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(9477..10196)
                     /locus_tag="NK767_002329"
     CDS             complement(9477..10196)
                     /locus_tag="NK767_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCH6695284.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT         3436 a         1881 c         1318 g         3573 t
ORIGIN      
        1 attcaatcat actgattctt tcgtgtattc aaaattagat gctgtttaat aaaattattc
       61 tttttgaaaa agaattataa ctatatccca aaaattgtag gttaatttaa agcctatttc
      121 atcatcgtga ttgagcttct ttttcataga cgaatttctc agatagttta gttttcttta
      181 tgagattttt gtatatttct gaccttcgat acaactcatt atgagtacct tgatcaacta
      241 gttttccttg atccatcact acaattttat ttaccttttc aatagacttc aagcgatgcg
      301 atataattag tactgtctta tcttgtatta aacgatttaa actttcctga atacgttttt
      361 cattttccgc atctaaactg gcagtaattt catctaaaat taaaattggc gcatttttca
      421 aaaaagcccg tgctatggat aggcgttgtc gctctcctcc agaaagtgaa cttccgtttt
      481 ctccgaccaa tgtatgataa ccgtcaggta gtttttcaat aaagtcttcg cagttagcta
      541 aacgagctgc ttttttcact tcctcatctg acgcttgact atttccaata cgtatatttt
      601 ccaaaatact tgtattaaac aaagttacct cttgaaaaac aatagcgatt ttgctaaaca
      661 aagaagctgg agatacacgt ttaatatcat acccatcaat taaaatacag ccttcatcat
      721 aatcatacag tctagacact aatttcaaga tactagtttt cccggaacca ctagcaccta
      781 ctaacgcagt cacttctcct tgtttagccg taaaagagat atgatctaaa attggggtat
      841 tgttatcata tgagaaagag acatctcgca actcaatgtc aaaagatttc agaggactat
      901 ctgatccctc ttggatactg gtctccttca tagctctaat tcgctgtatt ttaggagcta
      961 aataaaatat ttcaagcact gcttctttca tagaatcgaa ggaatccttt atttttattg
     1021 cggctagtaa gtaaccaacg acgtagagta tagtcacttc acccgttaac agtaagtgaa
     1081 cacctactag aatgactact gctaaactaa catatgagaa aatagaggat aaggccatga
     1141 ctgaaaaagt acttaattct acctttaaat gaatcctttc actctcttcc atttttttga
     1201 aaagtctgtc ttgaactttt ttagatagat taaagctatt aatctcttgc tgcaattcta
     1261 tagtttcttg aaattcttcc gagttttctc tcaaagtatc gtaatattta gtattggctt
     1321 tcgtttggga tttctttgat aacaagatta agacaaaact aaataacgtt ggcaaaataa
     1381 cagctaatcc cattttgaca ttaccaacca aaagcatcac tgaaataaaa gggaaaaaca
     1441 gagccatacc gccggcttta ggtattgcat gactcatcgc atgctcaata ccttcaacgt
     1501 cagacatgat agtttgagat aaatctgata aattatgttt tgaaaaatag gataatggta
     1561 aatttgacaa atcgtctgca atttgcactc ttaaatgcgc actttcttta taagtactat
     1621 tatataagtt ttcatactcc ctatctaaca accaatacat gaaaagcaaa gtaacaaacg
     1681 atacggcaaa ataaagccaa cggggctttg tattttctaa aactaattcg tctatcagca
     1741 tcattataat gaatgctggt accatattaa tgcagtatac aaaaaaacta gaaatagaag
     1801 ctttagttaa agcttctgcg ccctgatcac ttaaagcaaa tttgttttta taaaaacttt
     1861 tcattgtcaa gcctccaatc atttgcattc tcataattag tgaccaattg tttatacact
     1921 tgatgtttag ccattaattc attatgattt ccacgttcta ctattttccc ttctgacaag
     1981 acaagaattt catctacaga tttaatgctg gataatcgat gagctatcat aataactgtt
     2041 ttttctttta tcagatgttg aaaagccttt tgtagttcat actcattatc tggatcaata
     2101 gatgcactgg cttcatccat aataacaatc tttgcatctt ttaaaattgc tctggcaata
     2161 gctatacgtt gtttttctcc tcctgacaga taaactcctt tactaccaat taaggtatgc
     2221 tctctttcgg gaaatttttc taaaatcgat tcgcaaccgg ctaatcttag cgcgtcaaat
     2281 actttacttg ctggcgcttg aggatttgct atagaaacgt tatcgtaaat actctgattg
     2341 aatagtttcg tatcttgaaa gacaaaagca attgtgttaa taattgtgtc ttttgaatag
     2401 tgatttaaag gcttaccacc aattttaatc tgtcccttat ccacattgta gaaacctgaa
     2461 attagtttag ctatcgtact ctttccgctt cctgattttc ctactaatgc ataaattctt
     2521 ttttgttcta aaactaagtt gaaattttct aaaacacact cttctttgta tgaaaagctg
     2581 acattttcaa atgcaatgtc acaattttta atctgtgtct cgttcccata ttttagttgg
     2641 tcttgcttca tttctaaata aagggagtct agtgtttcga ctgcatatct tgcttcaaac
     2701 aagtgcatac ttacatacat tattctcatg aaagaaacaa aaataattcc acttaaaaaa
     2761 aacaacatca acaagtcaac agctaatatc ttgggagtgg taagagaagt tataaaaaag
     2821 gttataggaa ctatcaaaat gcaaactatg ccaaaaaata gccattgaaa aagaacaaaa
     2881 ggtgttttcc cactcaaaga atacttatat gcaaacttag cataatcgtt aattgcttta
     2941 ttcaaggctt taaatgatgt tactttagca ccaaatatct ttataacttg aattccgcgg
     3001 atatattcta ccgtttcaga gcttaacaca tctaacgatt cttgatattt ttgaataaaa
     3061 tttgtattct ctgtcatttt ctttaacaag aatacaccaa ttactaacat ggcgattaga
     3121 accaatccaa ttttaaagtt aatgaaaaag cctaaggcaa ctgataaaac aggtgttact
     3181 atggcttgag cactatctgg aatcaagtga gcaattgcca tatgtgtttt agccgcgttg
     3241 tcatcaattg tctttcgaat atatcccgaa gaatttaaat caaaaaaacg aaaacttgct
     3301 tctgttaaac cgctaattcc cttttttctc aaattagtct ctaagcgaaa cccaaggata
     3361 tgagaaaata agccagaaaa aatataaaat aaagctccta aagttaacca agcaacagtt
     3421 tgaaccgcat aactcttagc taaatattca tctcctacaa taattagaga ctgaagaaat
     3481 ttgtaaatac tgtaatatcc atagactgtt aatacgacag aaaaatcaga aaataaagtg
     3541 gctaaccatc cgtagtattt cattttaggg acataactaa ataatttttt gtatactttc
     3601 attttttcct cccccgcatt caatttcttc tcttattcgt ttacaactgt taatgctttc
     3661 cgtaatagcg taaattagag atgtacaaac agataaaagg taaactacgt tctatttttt
     3721 gtattttttt aagacttttc ttccaatcaa tacaccaatc atagataaac ttgctacaac
     3781 taacactgaa atgattcccc atatacctac cgcatagtgg atcattgcat ccattgttgc
     3841 agcagattgt cctttatcta tccaatcttg acgaaaacga tctgtaaaga accatatagg
     3901 aatccactgt ccggcagcac tgcctaattg cattagagcc caagacgcaa cttgaggaat
     3961 tgctcgatca tatccaaata cagatagaat aatatcagct aagatcgcca tactaatcga
     4021 actgataaac caaggtaagt acgccccgcc cattaacgta aaaaataaca ataaaacaat
     4081 tgtgtaaata gtaaaaattc cttttctagg gtagttatag ctaatataaa caaatatgat
     4141 tccagataat aatccaaaaa tcccggggga tatgctgtga ccaaacgccc caccaatact
     4201 actaaccatt attgaaaaaa agtaaattac tagggaaata actgtagcta aaccaataaa
     4261 aatataatct tttgttttca tatatattct cctcaattct atttcgttat gtcattaaaa
     4321 atctgttcta tcaaagttga atcttctact ctaagtttta tatctttttt tattccacca
     4381 ttttttaaat aaataattct attgcatgtt ttataaagaa attccggatc atgagtagct
     4441 ataactactg cacccttttc ttttaagtct ttaataaggt cagcaatcct ttccatatta
     4501 atataatcca acccacttgt aggttcatca aatagaaata aattagtttt tgacaatgaa
     4561 gctatagaaa ccaataatct ttgtttttct ccaccggata aatcaaacgg taattgatct
     4621 ttcattttat aaagttccat tttttttaaa attttttctt gctgatcttt actaactatt
     4681 tcattcccca gggctaactc gctcctcact gattctgtga aaaattggta atccatttct
     4741 tgcattacta aaaacggcga tgacaaattt tttttatttt cgattatctt tcctttggta
     4801 gggcgaataa tcttcatgag tattcttagt aaggttgttt tccccacacc gtttttccct
     4861 accaaaccaa taacatctcc aactttgaca tcaaaattta tgtcttttag aatattttca
     4921 taagtaattt cttcaacttt taatacatct gcacttacct tttcgatata gggaacttca
     4981 atcgaactat taaaaatgtc aaatttcctt aatgcgttat tgttttcagt ttttaacaac
     5041 tgaatagttg tagtttttac agttctattt ttaacaaata gaacttgatc aattaattct
     5101 ttcaaatagt aaaaacgatg ttcagctata acaatagtaa atcctttatt ttttaaatca
     5161 caaagtaagt cagcaagttt cttagtcgct ttgaagtcca aatttgaaga cggttcatcc
     5221 aatataatta atttagagtt tagcgataaa ctacatccaa tagaaatttt ctgccgttca
     5281 ccacttgata gcgaaaaaat gtttttatct aatatatttg aaagatctaa taattcagct
     5341 aattcattga ttcttttatc catatgctct tttgtaaatc caaagttttc catcgaaaat
     5401 actaactcac tcgttgtatt tgttgtaaag aattgtcctc taggatcttg acttacgtag
     5461 ccaatatttt ttgatatact ttcttgtgtt aaggattgta gcgagttatt ttcaaataaa
     5521 atttccccaa ccaattttcc gtcataaagt cttggaataa gtccattcat taactttaat
     5581 agtgtggatt tcccactccc agaagaacca gtaatcaatg taagactgcc tttttttatt
     5641 gaaaaattaa ggtcaacaag cgcttgttcc ttaacatcct tatatgaaaa agaaacattg
     5701 ttaaattcaa acaattcttt cacctcaaat caaaatactt aagaaaaaaa tagtaacgga
     5761 aaacaggatg atatagtcta ataagctcat gtttgtttta tagtaagaac ttcttttcac
     5821 aggagattct atcccctttg ttattgcatt agcggtcatt tcttcagcaa tcatagttgc
     5881 acgtataaga acgggaacta acagaaattc caaatattgc agaggctgaa aaaaagacaa
     5941 gcgaatgttt ctcattttta aagattcttt aatcattctt atttcagaac gataagtcgg
     6001 aaagaatctg atcactactg taatacttaa cattattttt ttcggtaaat gtattttttc
     6061 taaagaggat aataattcat tgctagtata gacactaacc aacaaccatg agatcattac
     6121 gaccggcaac attcgaaata caatatataa ggatgtcgca aaaaatatag taaaaaactc
     6181 tatcttgata gcatcaataa ttttcattac taaaaacaga actgaataaa tcaagatgaa
     6241 ataaatacat gtctttattt tacgaaacat caatactaaa actgtagcaa taatgaaaca
     6301 ccccacttga aatatatttc cctttcctaa aaaaataatg accggaaaaa aaatatttaa
     6361 agtaatcaag gttaacggat tgatttttga cactctttca cctccaaaaa ataagatcgc
     6421 actgttatct taagctgata aaaatgcacc gttattcaga taacagtgcg atcttatcaa
     6481 aaagcataac tgagaacaat tctcaatgca agcttttttt aattaactca gtaattcaat
     6541 ccacccagga acagaaaatt gatacaatct tctggttaat agcatagtct tttcttttga
     6601 atagctatgc ttcaatatat catagaatgt agcgtagaat gagtaaatgt agaagtggat
     6661 ttcttcttca ttaaagacta aattttgatt gatcattccg atagtcttta acttatctag
     6721 acaacagact gtctttttcg tcacaaatga tgtaattttt tcaacaatat cttgatattt
     6781 ggttccagct gattggaata aaatcaaatc gaatatacgt ttattctcaa aaatataatt
     6841 aataaatgga tctaattcat tgaagttctc tctccaactt ttctctatat ctatattttc
     6901 taagtttaag aggtatttgg taaactcttc attatacata ttaataagcg cattaatatg
     6961 cggctgaata atttcttcaa ataacgcttc cttagaagta aaatgtttat aaaaagcacc
     7021 cgtagttacg cctgcggtca ggcacagttg ccttatctta gtttgctgaa atccttcttt
     7081 ttcaaaatgg cttagtccac tttctattaa cttttggtga gttataatat aatctctggt
     7141 ttctatgggt acttcctcct aataagatga attaacttta ttatcttaca caggacttga
     7201 agtttcaatc gttaacgttt attactgaac tcattcctta gagctaaact aatgagaaag
     7261 agcagaataa ctgatacaaa aagcaatcct acaagaatta atagactcat ctaaatcatt
     7321 caccttttct aaaataatcc gagaaagatt taaaaaaatt atatccagct attaggataa
     7381 gtcctatcat aatcactcca atagccattt tcttatcctc cctaaaaata aagtctttta
     7441 cttaaaacct tttttgtaat cgtcaataaa ttttttccct ttatcagtta attgaaatcc
     7501 tatatctaaa tagtcctcat atctaagttt agccagcatc tcacttacag atgctggact
     7561 aacacttaga acttttgcta aatcattatt ttttaatttc ttttttcttt ttgataattc
     7621 ctgaattgct agtaaataaa ttagtatagt agtcgtttta atttgtacca tacctaccct
     7681 ccaaattatt tcgaaaggcc ttcagcaatt ttatccaggt tccatttcat catcgcatag
     7741 taactatcac catcttgtcc tttttctgca attgaatcag taaaaatcgt tgaatagata
     7801 ggaatattgg tatcttttga tactgttttc attggtctat catccacact gctttctacg
     7861 aataaggagg gaacttttgt tgtgcgtaat ttttcaacta agtgttttat ttgatctggt
     7921 gttccttctt cttcagtgtt gatttcccaa atgtaagcag aaggcacatt atacgctttc
     7981 gagaaatatt taaagcatcc ttcacttgta acaatcattt ttttatcatt cggaattgaa
     8041 gcaaatttag atttagcttc tttgtctaga gaatccaatt tttcaatata cttatctaga
     8101 ttttctttat agaatttttt attatcagga tctttttccg ctaactgttt ttcaatattt
     8161 ttagcgtaaa taataccgtt ttctaaattt aaccaagcat gaggatcttc cttccctttc
     8221 tcactctgac cctctaagta aataacatct atgccatcac ttgctgcaaa atagtctttg
     8281 ttttcctctt tgttcgcatt tttaactaat tttgtaaacc aagcatttcc tccagtttcc
     8341 aagttaacac cgttataaaa aatcaaatct gcttttgaag ttttttgaac atcttcaggc
     8401 aaaggttcat attcgtgggg atctttccca ataggtacga tactgtgtaa atcaatttta
     8461 tcttttgcta tattttcggt aatatctgct aaaatcgaat tagtaactac tacttttaat
     8521 ttttcgttcg aagccacttt atctttgtct ttactattta ttgaacatgc cccgaatata
     8581 aaaagagcaa tcaaaatccc cagacttccc agtactgctt tccattttct catcataacg
     8641 atctctcctt ctttcttaaa aatttttgtt ttggtgagat tagaaaacta attacaaata
     8701 agaatgccgc tgttaaaaca atacttgatc ctgctgctat gttaaaacta taaccaataa
     8761 acaatccaag caatgatgaa cttgctccga ctgttgcaga aataaatatc atagttttca
     8821 agctatttgc atataaatat gctgtagctg ctggtgtaat taacattgca acaattaata
     8881 tagttcccac actttgcata gctgttactg ctactaaagt taatagaacc atcaatagat
     8941 aatgataaaa actgacattc atccctattg cttttgccat taatggatca aaagaagtga
     9001 ttaataattc tttaaaaaat agaactacta taaccaacac taaaaccgaa acgccaattg
     9061 taatccactt atcaatatct tgtacagcta aaatatttcc aaataaaata tgaaataaat
     9121 ccgttgagct atttgcaact cctataagga taactcctag tgctaaaaaa gaactgaaag
     9181 taataccaat tgctgtatct ccttttatta cactattgct ttttataaaa gtaataatga
     9241 tagaagctaa tagaccaaat acaatcgcac caataaaaaa attaatacct aaaatataag
     9301 aaattgctac tcctggcaaa acagcatgag aaatagcatc acccataaga gacattcctc
     9361 ttaaaataat aaaacaacct actgttccag aaacaatccc tataactaca gatgtaatta
     9421 gagcattctg taaaaagtga aagtcataca gaccattaat aaaattttga agcatattat
     9481 tccccctctc caataaatat agtatctcca taggcttttt ttaaattttc ttcattaaat
     9541 acagattcta cacttccgtg agcaattaga tttcgattca aaagaataat attatcaaaa
     9601 tacttcttta ccttgcttaa atcatgatgc acaattagca ctgtttttcc ctgatgtttc
     9661 aattttttta acgtatccat aattattcgt tcactaacta agtctatccc tacaaatggt
     9721 tcatctaaaa atatgaaatc cgcattttga gcaagacatc ttgctaaaag tacacgttga
     9781 aattgtcctc cagataattc tcctatttga tgattagaat attttcccat atcgaccttt
     9841 tccaaagcct ttgaaactct ttgccattct gcatttttaa ttctttgaaa aactttcatt
     9901 ccggcatatg ttcccataga tacacattct tttactttta ttggaaaagt aaaatcaata
     9961 tcagcttttt gctctacata tgcaatcttc accagttttt tattgatatc ttcattatta
    10021 atggagacct ctccctgatg agggataata tttaacattg ctttaagcaa tgtggatttt
    10081 ccagcaccat ttggaccaat aatacctgtt aaagcacctt cttgtatttt taaagaaatc
    10141 tcgttcaatg ctaaaaaatc gttataacaa acattaagct ttttcacttc taacataact
    10201 ttctcctt
//
LOCUS       DAHUVV010000095        10032 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.098, whole genome shotgun sequence.
ACCESSION   DAHUVV010000095 DAHUVV010000000
VERSION     DAHUVV010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.098"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            255..734
                     /locus_tag="NK767_002330"
     CDS             255..734
                     /locus_tag="NK767_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695285.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            767..2995
                     /locus_tag="NK767_002331"
     CDS             767..2995
                     /locus_tag="NK767_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCH6695286.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3124..4767
                     /locus_tag="NK767_002332"
     CDS             3124..4767
                     /locus_tag="NK767_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCH6695287.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4840..5385)
                     /locus_tag="NK767_002333"
     CDS             complement(4840..5385)
                     /locus_tag="NK767_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCH6695288.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5408..5893)
                     /locus_tag="NK767_002334"
     CDS             complement(5408..5893)
                     /locus_tag="NK767_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCH6695289.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5883..6695)
                     /locus_tag="NK767_002335"
     CDS             complement(5883..6695)
                     /locus_tag="NK767_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HCH6695290.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6834..8366)
                     /locus_tag="NK767_002336"
     CDS             complement(6834..8366)
                     /locus_tag="NK767_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCH6695291.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8475..9506)
                     /locus_tag="NK767_002337"
     CDS             complement(8475..9506)
                     /locus_tag="NK767_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCH6695292.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3344 a         1850 c         1929 g         2909 t
ORIGIN      
        1 tacaaaaaag atgttggtga agttcaccaa catcaaaaag tatagagcca aaatattcca
       61 acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga gatggtgttt
      121 ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct ctataatgtc
      181 gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa ataaaaggat
      241 gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta atcgttgtag
      301 aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa gggctttcac
      361 tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac
      421 atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt attttagatt
      481 tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt tatgaaaaat
      541 tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac cgaatgacgg
      601 tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat tacgatacac
      661 tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt cttttagatg
      721 agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg agaatttctc
      781 actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga agaaaccgaa
      841 aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag ggaattggca
      901 agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt cggggaagac
      961 acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag tgacctgttt
     1021 tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag attctaataa
     1081 ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg aagctgtact
     1141 gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa aattagcaga
     1201 gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct attacccttc
     1261 tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc cgactgtcat
     1321 ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa
     1381 tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca ctacaaatcg
     1441 aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc aacatgcgga
     1501 aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga aaactatgat
     1561 ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg atgaaactgc
     1621 aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt gggcaaatac
     1681 ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt ataaattatc
     1741 cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg gattgctact
     1801 ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg agcatacaaa
     1861 agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga
     1921 aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga
     1981 gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa aagaacgcag
     2041 tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat
     2101 gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga ataaagaagc
     2161 gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc accgaccagc
     2221 tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc caatagtaga
     2281 atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga cgtaccaaaa
     2341 aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg atttgagaaa
     2401 ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg tacggatcga
     2461 tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc
     2521 cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag atacagcaga
     2581 ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag acggatttca
     2641 atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg atggtgctgc
     2701 acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac tgttgcttaa
     2761 tattagtgac ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag caaaagaaga
     2821 tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct ccgcagctat
     2881 tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg tagatattac
     2941 tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc tatgataaaa
     3001 taaagctcag cgaacgcact tagcagattc gttgagcttt tcgtttctag ttaccggtaa
     3061 caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa ggagtgaaac
     3121 gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat ctatcctgct
     3181 agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg tgaaaaactt
     3241 aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta tcaatctcca
     3301 tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag attcggaacg
     3361 atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa agtgattatg
     3421 gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc gaaaaaatct
     3481 aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg caaagaaccg
     3541 aataattgga catcgatttt cggcggttct gcttgggagt attctcatga aactggacag
     3601 tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag cgagaaattg
     3661 aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga tggatttcgt
     3721 ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac agagaaccct
     3781 ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt
     3841 tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc agaagaagga
     3901 ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga tcatatccat
     3961 atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca tgcattaagc
     4021 gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga aaaccatgat
     4081 gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc tatgtctgca
     4141 aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta tcaaggacag
     4201 gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc agtagattcg
     4261 aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc attggatatc
     4321 attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc ggaacaatat
     4381 gctggttttt ctacacatga accttggctg atcactaatc caaataccaa aacgatcaat
     4441 gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa catgatccgc
     4501 attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct tcatgaacat
     4561 ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat tatggtaaac
     4621 ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc ctggacatta
     4681 ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc accttatgaa
     4741 gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta gttggacttt
     4801 gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg ctctaggacc
     4861 accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa
     4921 acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg tgtcaccgat
     4981 atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga actggtcaaa
     5041 agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga caatttcgaa
     5101 attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat gttcacaacc
     5161 ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac ggatgatcca
     5221 ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt cgcttgtgct
     5281 gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata tgtcattaac
     5341 tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct aaaaccttct
     5401 ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg
     5461 caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc actccagccg
     5521 gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact aagcttccta
     5581 ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca gcaacatatc
     5641 cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat
     5701 cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca taaagaaaag
     5761 cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc ggtacaggga
     5821 tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag atcgttaatt
     5881 gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac acttccggta
     5941 tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt actttctgct
     6001 actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga agaaaaggca
     6061 caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac ctcataatga
     6121 tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc gcctttgatc
     6181 acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat ttctgttaac
     6241 gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc
     6301 gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt gatcacagct
     6361 tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg ttttgttttt
     6421 atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat tttgatacca
     6481 tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc caaagatata
     6541 gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa ggcgcttagt
     6601 ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc accagcaaat
     6661 ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat gaataagagt
     6721 ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct catccaattt
     6781 aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa tagttaggac
     6841 ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt agctgttcgc
     6901 gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc atggacttgc
     6961 tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat cggttgtgga
     7021 gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga atctaataaa
     7081 aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg ccgatctttc
     7141 aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca ccaatcgcca
     7201 taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg tcctttagac
     7261 agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga atctgtaatc
     7321 cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact tgttttcaaa
     7381 ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc atatacttga
     7441 agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt
     7501 atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg atgctcctta
     7561 gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat tggccggtga
     7621 gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat
     7681 cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg agagatgtga
     7741 aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc
     7801 catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt accagaagta
     7861 tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga caaagcaggt
     7921 tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga aatttctctc
     7981 actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg atcgataaat
     8041 acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc accaaggtaa
     8101 gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat aataactttg
     8161 tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac actttcgcag
     8221 cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag
     8281 ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa taagttcaaa
     8341 ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa gtaatataca
     8401 aattcaagat aatcgtaaca taatccttta tataataaca tagtttattg tgaaaaaaag
     8461 atcatacatt tgttttatat ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa
     8521 aaatcaacca tcttttccca gatatcttga aaagaatggg atgagaggta tcgattacca
     8581 cataaataat ttaccgcgtc agcatcatct aaatatagtt ctgcataata acgatatgtt
     8641 tttctgcata gttctggcga tacgttttta tatactgtaa ttttgctcaa aatttggcga
     8701 atagtccgag gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc
     8761 gtattttgtg ctgttaactt aaaatagttc tccagaaatg gcatcgcttc ttttggtatt
     8821 ggtactttat tcagtctgtt ttcttttcta ttcagattaa gaatctgctt ggtcaaagat
     8881 acatctttac gtttaagttc tactacttca tggatcttta atccgcaata cagaagaatg
     8941 gcagtaattg caaggttcct cggtctattc ttttcttcaa agctagcttc tctgcttgtt
     9001 tgaaagcctt caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata
     9061 tatcgcggag ctgctttggt tgtttccggt aaacttgtac ccaaaagaga aatctcttct
     9121 tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt
     9181 gaaatcccgt aatagctatt ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg
     9241 aacgaacgag tatgtcgata gattttagct aagaaattct gataatcttg tttttctaaa
     9301 tggataatca gctgatcatc cacttcttca gaaaatactt ttctcaaata atcagtaaac
     9361 agatgaaaat cttttgcgta ttcatagcga ctgcctaatg aaagggaaga acgattttct
     9421 tctaaaaact gttgaaagac ggaagggagc aagtgtgtac gttgctttaa cttttcttgc
     9481 agttgttttc tagttttgct gtccattaca aattccctcc aatagtgttc attttctata
     9541 ttttaccgca tttcatcaaa tgaagataga cagtttgtct tgatcttagg aagcaaattt
     9601 ttaactaagt actagctaaa agatatgaag taagaagata catttttagt tattttatta
     9661 gtgcagtcat tcttttgatc ttttaattga tttatcctct ttttagctat gtggttactt
     9721 catgagcaat gaaatggatt tggttcgttc taatggcagg aggctctttc ttttatttcc
     9781 gaaaaattag aaaacatttt ttcagaatcg attggctctc catttattca ataatggttt
     9841 gtctaacgat attgttggga taattcttaa agaagtatta gaaatagttg gtacttcatt
     9901 actttacatg aagatttatt ctatggtagg agcaattata ttgagaatta atcctttttt
     9961 atataagttg tttgcttatt gatggattgg ataaattaaa ccatcacaac gagtggaaat
    10021 tatatgaaat tt
//
LOCUS       DAHUVV010000096         9735 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.099, whole genome shotgun sequence.
ACCESSION   DAHUVV010000096 DAHUVV010000000
VERSION     DAHUVV010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.099"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..205)
                     /locus_tag="NK767_002338"
     CDS             complement(<1..205)
                     /locus_tag="NK767_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695293.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(221..835)
                     /locus_tag="NK767_002339"
     CDS             complement(221..835)
                     /locus_tag="NK767_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCH6695294.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(982..1665)
                     /locus_tag="NK767_002340"
     CDS             complement(982..1665)
                     /locus_tag="NK767_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCH6695295.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1774..2232)
                     /locus_tag="NK767_002341"
     CDS             complement(1774..2232)
                     /locus_tag="NK767_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCH6695296.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2316..4079)
                     /locus_tag="NK767_002342"
     CDS             complement(2316..4079)
                     /locus_tag="NK767_002342"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCH6695297.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4300..5760)
                     /locus_tag="NK767_002343"
     CDS             complement(4300..5760)
                     /locus_tag="NK767_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695298.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="NK767_002344"
     CDS             complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="NK767_002344"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCH6695299.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6917..8344)
                     /locus_tag="NK767_002345"
     CDS             complement(6917..8344)
                     /locus_tag="NK767_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6695300.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8375..9589)
                     /locus_tag="NK767_002346"
     CDS             complement(8375..9589)
                     /locus_tag="NK767_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCH6695301.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2880 a         2032 c         1619 g         3204 t
ORIGIN      
        1 atttgttaaa gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac
       61 catctaaagt aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact
      121 gcccttgcag aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg
      181 atggtttata gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg
      241 tgaaaatttc tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt
      301 ttcttggatc gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct
      361 gtacttctag caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt
      421 catataagga tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta
      481 ctgaatcagt taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg
      541 ctgctgtttt atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc
      601 ttgtctcaag aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca
      661 aggataagat gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca
      721 ctatatcttt tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa
      781 tcagcaactt gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt
      841 cgccttcttt ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg
      901 cttttttcat cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag
      961 aatctgtgcc gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt
     1021 agtgatcaat tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc
     1081 atatgctttc tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc
     1141 gacttgttct atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata
     1201 ttctgtatag cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag
     1261 cgcttcctca aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg
     1321 gacaagttca gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat
     1381 gatttctgct tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac
     1441 ttctgaccca tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt
     1501 gatttcattg atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc
     1561 agccaaagcc attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg
     1621 acaagaaacg acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt
     1681 aatgttatat aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa
     1741 taatgagcta tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc
     1801 gtatacgacc agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt
     1861 aaaaaactca gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt
     1921 ccaagcatgc tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat
     1981 gatacaaact ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa
     2041 aggctcattc tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt
     2101 tacaactagt tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc
     2161 taatttttct gattgataac taggatattt ttcttggaac tttttgacat attcttctac
     2221 ttgctgatac ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta
     2281 gtataaacaa ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga
     2341 ctatcttcaa cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga
     2401 acgctatccg tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca
     2461 taataacgac tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg
     2521 actaattcgt gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt
     2581 cctggattga tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt
     2641 cctaattcaa gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt
     2701 tgataggctg gaatactata atcatttgcg atagctgtac catgttctac accatgtgta
     2761 ggaattcgga ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc
     2821 atatcctctt ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga
     2881 atgtcaaatg ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa
     2941 ataatcactg gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc
     3001 aagaaagtac ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg
     3061 aaagtgatag agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct
     3121 tggaatgttt tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct
     3181 gtacttgttt gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca
     3241 gaagttactt gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc
     3301 attttattat cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca
     3361 atcggatgat caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt
     3421 ccattatcca cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta
     3481 ccgttggttt gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata
     3541 gacatctcat tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc
     3601 aatccttcaa aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt
     3661 cctgattctt tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta
     3721 gatgtgtgcc ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc
     3781 acttcacggt aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca
     3841 taagactgat gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga
     3901 aacgcaatcg ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg
     3961 tccttttgca agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat
     4021 aattttttga catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata
     4081 atacattctc tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct
     4141 attctactac ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc
     4201 ttgttatata agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa
     4261 ttaaaaagct ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa
     4321 taggtgaagt tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg
     4381 atagatcttt gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac
     4441 cactcttttt ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat
     4501 aggatttatt tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa
     4561 ttgtttgact gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc
     4621 actgttgatc aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag
     4681 ttggatttga tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag
     4741 ttgttctttt tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca
     4801 caacgtacgg acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat
     4861 ttcagcgata ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa
     4921 atttttataa acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt
     4981 tctactgttg aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc
     5041 tttttgctct tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt
     5101 cttatcttct tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc
     5161 tcgtatgata aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac
     5221 ataatcaatc aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc
     5281 ccaattgaca taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat
     5341 gatttcttta tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat
     5401 tgtatatact ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat
     5461 accattaaaa taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt
     5521 gagctcagaa agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac
     5581 ttgttctttt atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt
     5641 tggtgtagtc acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa
     5701 taattgttca cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat
     5761 tcctgataca cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat
     5821 ttttgtttcc tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga
     5881 caaaagtcaa acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac
     5941 ttttacccca gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac
     6001 tacatctttc aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct
     6061 gaagacacct atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct
     6121 ttttgcaggt acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt
     6181 tgtcataatt gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca
     6241 aggagcaatt cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac
     6301 attgagggag cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat
     6361 ttgattacct ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga
     6421 atgatagatt tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga
     6481 caagattcgc tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat
     6541 cgctcctgca ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat
     6601 gatagaattg aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat
     6661 tccgttgtct ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa
     6721 gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat
     6781 acctaattgt ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt
     6841 ttgataatag tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat
     6901 aaccagcttt cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg
     6961 taaatggaat aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat
     7021 gctgtccagc tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag
     7081 tcaaaagacc taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg
     7141 ctctttcaag actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt
     7201 acaacatcag gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc
     7261 ccccgccgct taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca
     7321 tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt
     7381 cttctgctgc tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga
     7441 tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa
     7501 tgtgtcttaa taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt
     7561 gaggtactgt agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa
     7621 ccaatacaaa cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac
     7681 tggcagatgt ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag
     7741 gcaatttcga aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc
     7801 ctgaagcgac ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag
     7861 ctacaaaacc gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta
     7921 aaatagccca gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag
     7981 caccaatacc cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc
     8041 caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg
     8101 tgcgactgac ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg
     8161 ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta
     8221 agctgaagcc tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg
     8281 tcatagccaa aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt
     8341 gcatgaatct tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga
     8401 aaaagtccaa tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg
     8461 ccgattttaa aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa
     8521 tcacttgaat ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg
     8581 caaagatccc tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat
     8641 caagtgcatc atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac
     8701 ccgcaatgat cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa
     8761 tggctgccaa tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt
     8821 ttacttttgc aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac
     8881 caaatgcatt gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc
     8941 agccaaacca aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc
     9001 tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc
     9061 cagctgtcgt gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa
     9121 atccgccacc ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa
     9181 caagtataat atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa
     9241 cggtaatcac gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac
     9301 catgaaacat gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc
     9361 caataacacc gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa
     9421 tcgttgttat cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc
     9481 gtgctaatcc gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac
     9541 ataaaatcat gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct
     9601 cctttctacg attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga
     9661 attaatactt gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt
     9721 tttcgttttt cacac
//
LOCUS       DAHUVV010000097         9638 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.100, whole genome shotgun sequence.
ACCESSION   DAHUVV010000097 DAHUVV010000000
VERSION     DAHUVV010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.100"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(345..1052)
                     /locus_tag="NK767_002347"
     CDS             complement(345..1052)
                     /locus_tag="NK767_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695302.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1117..1953)
                     /locus_tag="NK767_002348"
     CDS             complement(1117..1953)
                     /locus_tag="NK767_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCH6695303.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1971..3380)
                     /locus_tag="NK767_002349"
     CDS             complement(1971..3380)
                     /locus_tag="NK767_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCH6695304.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3394..5217)
                     /locus_tag="NK767_002350"
     CDS             complement(3394..5217)
                     /locus_tag="NK767_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCH6695305.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6060..6386)
                     /locus_tag="NK767_002351"
     CDS             complement(6060..6386)
                     /locus_tag="NK767_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695306.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6432..6794)
                     /locus_tag="NK767_002352"
     CDS             complement(6432..6794)
                     /locus_tag="NK767_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCH6695307.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6831..7046)
                     /locus_tag="NK767_002353"
     CDS             complement(6831..7046)
                     /locus_tag="NK767_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6695308.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7173..7508
                     /locus_tag="NK767_002354"
     CDS             7173..7508
                     /locus_tag="NK767_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6695309.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7584..9581)
                     /locus_tag="NK767_002355"
     CDS             complement(7584..9581)
                     /locus_tag="NK767_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695310.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
BASE COUNT         2983 a         2014 c         1437 g         3204 t
ORIGIN      
        1 ataaaaaaac agtactgtct agtagtataa aatatacaaa tttcttaaaa attttttcac
       61 taaaatcact acctgaaaca gtctaaaaca cttatatatc aacgtttaat gaaactatct
      121 ccgtttcatt tcagatacaa ataaatatag tcgggctcca aatccgaaaa aaaatacccc
      181 cagtagctta tcctaagcta ctggagatgt ataagtacgg tcatataaca acggggtagg
      241 gtaaataaat gaaaaagatg gaagaatcat tattcaataa cttttttaaa tacttcgttg
      301 taatcagtta cctgtccgtt cttaaatacc acagaataat ctttttaccc attttcaagt
      361 aaccgaagtg gaatgtcttt accaacttgc tctgtcactg cattactgat acctgttaga
      421 ttagctaata aatctgataa agattgagca tctgtagaaa tctctgtatc agtatttatc
      481 gtgattcctt tatcttcatt tacaagcttc caagtaggtg gtatagctgt gtatgtttcg
      541 atgaatgaat caattctttc atctatcgtc ttcccagtat ctacttctgt ctcattactc
      601 ttagatgaat cgattctttc atccatcgtc tgctccgtaa ctgcttctgt ctcattattc
      661 tcaccggaaa ctctcttcac acccaaactt atatattgtc catctttagt gtgcatagtt
      721 aaaattccca cgcttttact aataaattca ctataaccag ttctttcaat agagacttca
      781 atgtatttca cactatcata ctcaaaagaa aaatagacat gtaaaagcat tttgttgttt
      841 acatctacat aataatcctt tgactctact tgagaccttc ctatacggaa tacagcttta
      901 ctgtctgata tctctatacc agcatcctta ttattaatct gccaatctcc aagtagctct
      961 tctggcaagc tagtagtttc ttgtgaacac tttaccactt cactggtaga ggtcgaaaca
     1021 ttcacctccg cactgttaga agaattttcc aaggaatcag gagccttagc acaaccaaca
     1081 agtctttata tattgtgcag ccgatatttt cttcattcag tacacccttg aaatatgcag
     1141 tgttagataa agcagttcgt cctgatttgc tgaggcaccc atctcttctt gaacgtactc
     1201 cttgattttc aaggccgccc gatactcttt cggaaaacgc tgcttcactg aagccagcaa
     1261 aaaatcatcc ttctccggca actcttcctt cttaatcatt cgggcaataa aaaactgtaa
     1321 atgcgtcaca aatcttaagt aaagcatcga atctttatca accttttttt cgggaaacag
     1381 atatcctagc atagccaaga tatcctttag tgctcttacc gagagcatgg tttcttccat
     1441 atttgcttgt tcactttggg catttacaat gtgaaacgca atgtttccag cctcctcttc
     1501 tggcaactga acattcattt ttttgttgat caattggata gcatagcagc cgatctcaaa
     1561 ttcttttggg taatactttt gaatttcaat cagcaggcga ttttgaaaaa caatgccttt
     1621 ttgactgcgt tcaatggcat gcttcaagtg atcaaccaag gtaaaaaata gacttttatt
     1681 cagcttaaag tcaaacttgg cgttggcata atcgataatt cgcttgacgc aaataattag
     1741 atcatccgga gcagcctcaa acagtttaat gtaatccttt tttagagcat cagaaagtga
     1801 atctaattta aatacttttt ggattttgtc agccggaacc aaatcgcctt tctttagttg
     1861 aaatccaatc cctttcccca tcaaaattac ttctttgcct tgcttatcta agcaaagaat
     1921 taaactgttg tttaacgttt taattactct cattagattc accgttctct ctaagctaag
     1981 ccatttcttg cgattacttt ttgataccaa aagaagcttt ctttttttat tcgttttaat
     2041 tctttaagat tctcatcggt tcggtcaaca tatataaaac cataacgctt attgaagcct
     2101 tgatgggtac taacaacatc aatcgctgac caaggtaaat aaccaaaaac atctacacca
     2161 tctgttaaag ctgcttgaat agccagaata tgtttggcta gataatcaat tcgatcctgg
     2221 tcattaattc gtctatcttc ctcaactatg tcgggtgccc catagccatt ttcagtaatt
     2281 aaaataggta aatcatagcg atcgtacaat ttcctcaatg ttaatcgtag tccagtaggg
     2341 tcaactaccc agccatatgg cgttttatct acataaggat tttcagctgc ccggtagaca
     2401 ccttcttctc ccaacatgat ctgttgatcc cccgctctag cggaaacatc tgatccgtca
     2461 tttttgctgg cagcgatggt ggcagttgaa taataattaa tcgcaataaa atcgggttta
     2521 ccggaggata aaattgcctg atcctctgcc tcaattttag gatataatcc acgatcattc
     2581 aaatagtttt caaacaaata attgtatttt ccccgaactg ctacatctaa gaaagaccag
     2641 cctctcaatg tttcccaatt atgggctgca attgcatctt ctggactgga ggtagcttga
     2701 tacatagccg ttaaattgat tgctggaccg atttttcctt taagattcaa ctgatgatac
     2761 aagtgaatta ctctagcttg agctaataaa acatggtgat taatttgaaa gagttctttt
     2821 tttgaaggca gctctccact tggtggcaaa ccaatcgctc caggatgaag gatcatcgtg
     2881 ttttgctcat taattgtcag ccaatagttc accttgtcac catactcagt aagcaatacc
     2941 tgtacatagt ccacaaaata atcgatagtt gctcgattta accaaccgcc ctgctcctct
     3001 agaaaataag gtaaatcgaa atggtacatg gtgacaatcg gttcgattcc ctgattcagg
     3061 cattcatcaa ttacttgatg ataaaaagaa attccctttt gattggtttt attattttca
     3121 atatccataa ttcgcgtcca agcgatggaa aagcgaaaag cttttaagcc taattcttta
     3181 aacagctgta tatcttcttt atagtgatgg taaaaatccg aggcaacttc aaaattagct
     3241 accccttctg tatgctgata cttgtcaata atagacggtt gtttcccatc ctcctgatag
     3301 gcaccctctg cctggtaggc actaatggag gcaccccata aaaacttctc tggaaatgct
     3361 gaaagctgtt tgtattgcac aaaaatcctc tccttaacta atttgcatca atggttcgtg
     3421 ttcctttaag cttccgcttg cttcactaat tcctgcgtaa ttttcactgt tggtcactac
     3481 cacaatcact gtatcatcgt agccagcgtt tctaatggca gcgaaatcgg cgttgattaa
     3541 taattgtcct gctgacacct gatctcctgc ttttacaaat acttcaaagg gttccccttt
     3601 taaattcacg gtgtcgatcc ccacatggat caataattca ataccttctt tagaacgtag
     3661 accaatcgca tgtttagatt ctaaaatagt aatgacttct ccctcaacag gtgaataaat
     3721 gctgttttct tctggatgaa tggctacgcc ctttcccaat agtcccttag aaaagacagc
     3781 atcgttcact tcagaaatag gacacacggt tccttttacc ggattttcaa caatgactgc
     3841 ttcggctatt gccgtttgct ctgattcatc agaagtatcg tcaaatccaa ttagataagc
     3901 aatgataaag gttgcaacaa aggcgattac acacgtaata attgcgtgaa caatattcat
     3961 tggattttct ccaataaagg ctggtaaggc tgctaagcca ggggaaacaa aggcatatct
     4021 gactaaaccg ctcaagccag catacaaacc agcaattgca ccaccaatca tggaagcaac
     4081 taatggtttt ttcagcttca aggttactcc atagagtgaa ggctcagtaa ttcccattaa
     4141 agcagtgatc ccagtggaca cagctagctg cttcaactct ttgtttttag ttttcgcggc
     4201 tacagcaaag gtagcacctg attgagcaat attagaagcc agcattcctg gcccattgat
     4261 catttcagaa cccgtactgc tcaattgcat agtagcgatt ggcgtcattg cccaagctgt
     4321 acccgtaacc accaaaaatg gctgcaaacc acccatcaaa aatggaatta accaagctgc
     4381 tttgccgtta atccattcag cccccattgc tataagatcg ttcagcaacg ttccaaacgg
     4441 tccaatgaca accaacgcta aaggccccat aattaaaaat accagcatcg gcttagtaaa
     4501 gaatttaata aacgaaggcg aaattcgatc cgcaaagggc tctacatagg acatgatcca
     4561 aacagtaaag ataatcggta aaactgatcc cgcataatct gcagctaata ccggaattcc
     4621 tagaaaagaa acggcatcgc cagtagaaaa aagctgtgaa atatttggat gaagcaaaat
     4681 gccagcaaca gtaatcgcca aaattgggtt gacatcaaac tttatggctg ccccataggc
     4741 taatagtact ggcataaaat aaaaggctgc atcagcaata gtattcaaaa tgatgtaagt
     4801 ttgactactg tctgatagta caccagtcaa agtcaagacg gccaaaatgg cttttaccat
     4861 cccggcacct gttatagctg gaatcatcgg tgtaaaggtg gtagaaatga cactaatcaa
     4921 tcttgaaata atactttctt gattgtctga agaggggccc tttttaattg aagttcccac
     4981 ttccttttga atttccttga aggctgtttg gacttcattg ccaatcacca cttggtattg
     5041 tccaccgcta tccaccacac taattacacc aagtgttttt tctaaggctt tcgtatcggc
     5101 tttcctttta tctttgagtt caaaacgcag tctggtggca caatgggtta agctgaccac
     5161 attttctttt cccccaacct gttgcaatac atttcttcct acttctctgt aatccattat
     5221 tctctctcct taaagcagta aaaaagacca agtcaaacca cgccccaatc aacattgaag
     5281 tgttagtttg tcttggtctt gcctgcataa tcagtaacat gccaataacg ttattttgct
     5341 gtaatccaat aatagcacat tatgatagcg caatcaacag ggaatttaat tttttatctt
     5401 ttcgtttgac cgtatagggt aatataacag ttagatcaca tcaactaggg tatatctgcg
     5461 tatacttact gctaaaataa gtatatctaa tattaactat accagtaaaa ccattcctac
     5521 ataaccagcg ggaaattccc aaaatcgtga aagcatcaaa aatgcatata acggatcaca
     5581 caacttcaac aattctgctc ttgggaaaaa ttgatgaatt tggtgagtaa aggatggaat
     5641 aaacagcatt ccaacaatat aggcaacaat agtgatactt gtttttttgg taaaaatcag
     5701 taagcaatag ccaatttcaa tcattccgag atataaaggt atcgatagaa tgatttgggc
     5761 tagtattctt aaaaattaac tagcaaatgt ttaaactgat ttatttctaa tctacatgta
     5821 gtaagtattt caattccacc aattatcaat aaaaatacca aacatatcca taataatttt
     5881 ttctttttca tttcttttat attgttataa cagtgaataa cataaaaatt cccgacaatc
     5941 attgtaatat ttttttgtca cctgtattag tatttaattt ataggaaact ataaaaactc
     6001 cctagtgaat ttacaccttt gggagttcct gtttggacgg tatgcataat acatttaaat
     6061 caatttaaca ttaacacctc agcatatggt atctccacat atatacggtc tttctttgga
     6121 tagtactcaa tatttatgtt gatagcactt ccattgtcta atatagcttt agcccggtat
     6181 gtgttatctc cattttcatg agtaatcgtc acatcatcac atgatcgatt taagttgtac
     6241 tgttcttgta aaatctgtgt taccaatggt tttgatgact tctctaatcc agatgtacct
     6301 gtcatttttg aaaaaataat acctataata gccaagacaa caatacctat aatgacaaac
     6361 ttcttgttca tatttacttt attcaacttt ttatctccta tctccaaaat aatttgttct
     6421 tatccctctt atcagtacat tgacatccct aaaagtacac caaagattgc tccagctacc
     6481 cctgctatca tcaaaatcgt acccattttc tcatcttttt ctcgatataa gtagcccata
     6541 ataacagcaa cagcaccaaa tacaacagga ataaatgcta acgatactac catagaaatc
     6601 caaccaataa tcaaatacag cttccctgtc tcttgtccat gtacgatttg ttccttcatt
     6661 tcattaattg tctgctcagt acttttcttt actgtgtctg ttccggttgt ctcatctgtt
     6721 tcttgtagtg ctccacaaaa ctggcaatac ttactttcat ctgttatttc tttgccacat
     6781 tttctacaat acatgtcttc tcctcctaaa atcattcaat tgcttattag ttaactgttt
     6841 actttattaa atacatcaga aaacagctca ttcattggta cacttaatat ctcagaaagc
     6901 atcttcattt gatagggttt aggaatcgtt ctccctgttt gccacgaact tacacattgc
     6961 tgtgtaacat tcattttttt tgctaactcc gtttgagaaa gtcctttttc ttttctgtat
     7021 cggctgagat tacttacttt tttcatgatt ctcccctcct gttttattat atacaacaat
     7081 atgatgtatt tcaactcata aaatataaaa aacttcaaat accattacaa atacaacaac
     7141 ttgttgtact atactgagat gggggtgtat taatgaaaca gaaacaatat gcaaaggtca
     7201 ttagagaaaa gcgaaaagct ctcgggtgga cacaaaaaga attagctgaa aaaatcttct
     7261 ctacacaaca ggcagtagca agatgggaaa attctgtaac agagcctaac ctcgatagct
     7321 taacagctct ttctagagcc ctaggaaccc ctgtaagcca ttttctagac aatgttgtcg
     7381 tagattatga ggaagagttt ttggctctct atcgttctct tagcactgaa gatgcagttc
     7441 gaacaattga ctatatgaag ctattaaaaa ggcaagaaaa tgaacgcaat cagctattga
     7501 aagattgaaa aataaataac ccaccgaact ttaacggtgg gtgtattgag atgaaataag
     7561 attaagagct atggataacc tgtctagttt aatacaattg ggtcttttct cttccttact
     7621 ctccgtccat catctcttgt tacacttgta aattgataca gaggaactat cccagctcct
     7681 gataatatca attcttttat tgtttcatct atccctgccc ctttcgctaa atatattaaa
     7741 aaactactac ttgctttcac attgtatagt ttcttaatat ccttagaagc tcgttcaaga
     7801 gtgaatcctt gtttaacaaa gtagcctctg ttttttatta aagcattttt cacgctgtta
     7861 tccccaacat tacgtaggcg ttcacaaaca gtgttaataa gttctttctc tgtcattaga
     7921 tatgtccctt gccacgataa gccctgtaaa acttcatgtg cttcatctaa aatcaattgt
     7981 ttttccttgc tgagttctga tcgggaattt tgaggaaata ctcttttagc ctctttttcg
     8041 ttaaaacata tcttaaccca ttcataagaa aaatattgat atgtaaaatg aattttttcc
     8101 agaagtggaa ggatatcttc attcaatttt tgaatcacag aagttgcatt aaaaacgtct
     8161 aatcgataga cattgcttct cttatgatta aagtttccta acgctttctc ttcctttaac
     8221 ctatttaggt attcgagcat cttcaaagga acatcttcat catctaattt ttttatcaat
     8281 cctataaaag tcataagtga tagtaactta ttacatttat ctatgtcata agttttacca
     8341 tagtaagaac tcattttttt agctaattgt tcccccgaca aaatacaagt cacactttcc
     8401 ttactatcat caaaaaaatt aacatcagaa agcaagacat catagacacg tcgcatttcc
     8461 tctagataat tccttattgt cctgaaaagt ttgtctttca ttgattcaag agtgtcaaaa
     8521 agtacttttg attcctcttt caattttcga aaatgtgtag gatacaagtc taggttctct
     8581 aatagccaat taaatgtttc tacccgactg tctttcttta aaattagcat cacaactcca
     8641 ataaaatttt cactgatatc acaattactg gaatgacaat aaaaaatgga atgcccagtt
     8701 ttactggtaa aaatactagc agagggatta tgatcgttgt gaaacagaca tcttaagttt
     8761 ttacttgact taagtaattt attcatatca atggttaaca atctttcata aatctcatta
     8821 aaagacatgt cggaggaatc aaacaggcag tctttaaacc actgtttcat ttcttctttg
     8881 tcgtcatttt ttatcaactt aacaaaatcg gtttcattga ctagttcata ctctctccct
     8941 gaatcacttt gacaattttt agaacatgtc atggtaaaat caatcggaag ctcagtaata
     9001 tctaacatgt tgtcttcatt aattattatc actgcatctg acttatatcc tccaaaaaac
     9061 attctattcg aagcatttac attgtcatca cacccgggga tgttcttttt caagtattcg
     9121 taaacagcaa ccattttacc atagtcagta attgcttctt ttaatagaaa gacaatcttg
     9181 tatttttcta gattttcaga gtaccgaata cttttttgta tcgcaaacgc agaattatga
     9241 actaaattga tagataaaac cttttctatg cttaagtatc tactatcatc tttactcaaa
     9301 tctactacct tccccttctc atctttaaca gtgttatcga catccagaat aattactctg
     9361 gtttccacaa aactctcctt gtcattgcct ttattctttg tcttgcttgg aataaatgat
     9421 tgtgatgaat ttagtatctc tacaaactca tctcttgtaa cttctctata gttatctaaa
     9481 cacctatctc ttacatcagt tttttctttt tttgtaactg gcttctctaa taatttttct
     9541 tgatctacat aaattggaat attaatcatt gatgtttcca tttgtgtttc ctccttataa
     9601 aattctgtta aagactaaaa catacagata ataaaaac
//
LOCUS       DAHUVV010000098         9538 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.101, whole genome shotgun sequence.
ACCESSION   DAHUVV010000098 DAHUVV010000000
VERSION     DAHUVV010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.101"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            3..608
                     /locus_tag="NK767_002356"
     CDS             3..608
                     /locus_tag="NK767_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCH6695311.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            628..990
                     /locus_tag="NK767_002357"
     CDS             628..990
                     /locus_tag="NK767_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695312.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            993..1175
                     /locus_tag="NK767_002358"
     CDS             993..1175
                     /locus_tag="NK767_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695313.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            1192..4611
                     /locus_tag="NK767_002359"
     CDS             1192..4611
                     /locus_tag="NK767_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCH6695314.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            4662..5399
                     /locus_tag="NK767_002360"
     CDS             4662..5399
                     /locus_tag="NK767_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCH6695315.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            5409..7700
                     /locus_tag="NK767_002361"
     CDS             5409..7700
                     /locus_tag="NK767_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCH6695316.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            7724..>9538
                     /locus_tag="NK767_002362"
     CDS             7724..>9538
                     /locus_tag="NK767_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695317.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGNPNLMANINADSFS
                     QGSGALSVVDDGDEVVVTLDPNNKLEIFKAKSQPALVVGKTYTMSVEIMLEGDFTGDP
                     SKIGLRYIKMPNWVSGLYTRNTLTATKGVWQKLTGTVKITAASDNAESWFIMLQNKDA
                     NNSLSGKLRLRHAKLEEGSTATPYQPNLLDAPYYLSKVALGENIADPAVSFPIKTSAY
                     RLYGVNMLEEFKVGQRYTITIKGTKPATQDFWAYNGGNISLERMTPVEGLVDVWTCSF
                     TILKLDSSSPSLLSIYQTPQSTVGSCQIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTG"
BASE COUNT         3342 a         1466 c         2032 g         2698 t
ORIGIN      
        1 caatggctaa aacaacaaca gtaacaactt ttgacaatat aagtattaaa agaatttctt
       61 ttaattttaa gaatgcaaca aatgcaattt caactgattg taatggacaa ttagatggcg
      121 aaacagaaat gcagagaatt gtaaaaaaat gcggttcaac agaagtaaaa tcgaaatcta
      181 aaccaatcaa tatgacggta acaattactg cacatgtacc gatggaagtt tatcgacgct
      241 tcaatgggtt gaaacaagat gaacgtatta aatcgggaat ttactcttat ggtcctgatt
      301 ccgtagggga agatttctct cttgctgcag agatcgtgga tgatttcgaa gaaaaaaata
      361 agctgattgg tatgttagca tgcacttcga atacaggatt aacattctct attgaaaatg
      421 gtgcggatga agtagctgcg ttagaactag aaacaaaagt tatgcaagat gaatttggta
      481 aattttatca tgaagcaatt gttgcagaac ttgaagaaga cttaacagat caatggatga
      541 cgaatctatc tgctgatgtg attaaaaaaa cttcagttgt gacaactacg gccactcaat
      601 cacagtaaaa aaaacggagg tagcgaaatg aacgaagatt actcaaaaat tgaactaaac
      661 gatggaacaa ttttgaattt agaacctaaa ctgaatatca agaaattatt gatgatcaat
      721 agagatttta acacagacga gtttgcaaaa atgactgtgg gaaaaggatc catggatatt
      781 tctgttattc aaggtgcaaa ggctgtgtat attgcttacc gccaagcgaa catgactgat
      841 tatatttcat tcgatgaatt tatcgataaa tgggattttg atatggctac tgccagctat
      901 atttatcaat tgatgatgtt caaacaagca cgcgatgctt atcaaaaaga atttgaaaaa
      961 gcaaataagg aaaaaaagct tcaaaagtaa aaatgccaaa gctcttagtt gaaacgtggg
     1021 tcgatgtcta ttcgatgttg accgacgttt tttctatgcc ttcagatttg gttttaagcg
     1081 atatctgttt agatgacatt ttgcaaatgg cttacaacaa gagtgcttat gaaggatgga
     1141 aaaactatgc aataaaccaa tcccagaaaa actaaagaaa ggaggtaaaa aatggctaaa
     1201 aagagaacag aagcagaagt aacattcata gctaacgatg acggattgaa atctacgtta
     1261 aaagaaatca gtgctgaatt aactaaaaat agagcagaat taaaactaga acaagctcaa
     1321 ttacaacaga ctggttctga atcagacaag ttaggaagta aattatcttc tttagaaaag
     1381 cagtatgaat tacaaagtca aaaagttgaa gtaactagcc aacgtttagc caatgccaaa
     1441 aaatattatg gagaaaattc caccgaagtt cagaaacttg agagagaact gattaaccaa
     1501 caaacagcgc aacaacgttt gtcaaacgaa attgataaaa cgagtaatgc actagctcaa
     1561 gcaaaaggcg aaatacagac gtacgagtct acaatgcaac agttggatag tgaacaaaaa
     1621 aatgttcaag ctagtgcttc tctgattgaa tccgaataca aaaaatggca agcaactgct
     1681 ggtcaatcag cttctgaatc cgagaaatta gcgaaagccc aagaatatgt ttctcaacaa
     1741 tctgaaaatg cagagaaaac gatagatatc ctgagacgac agttagaagc tacacagtct
     1801 gagtttggcg ctacatccac agaagcaatg cagatggagg cgaagcttaa tgatgctgaa
     1861 cgtgaatttg aagagttagg acaagctgct aaaaatgtag atacaactaa cttggacgat
     1921 atcggaagca aaatagatat gaataatcta atggaagctt ctgacgtttt aagcgacatt
     1981 ggcgataagc ttacagaatt agggaaacaa gcagtggact ctgctaatag tgtaggtagt
     2041 tcccagagta aaatacaagc taattttggt ttgactaaac aagaggctga agaattaacg
     2101 aatgtagcca gagacattta ttataaaggt tttggagaat cgttagatca gtccacagat
     2161 gcattgattt tggtaaagcg taatttaggc gatttaaata atcaagattt acaaaatatc
     2221 acggaacaag ctatggtcct agaaaacacc atgggcgctg atatggatga aacgttacgt
     2281 ggtgtaaatg gcttaatggt caatttcggc ttgagcgctc aagatgcaat ggatttaatg
     2341 gtttcgggta ctcaaaacgg tttagataaa acgcacgaat taggcgacaa tatggcagaa
     2401 tatagccaat tatggagtca aatgggatat tcagctgatg aaacgttcgg aatgcttcaa
     2461 aatggtttag atgcgggtgc ttataacctt gataaagtca atgacttagt taaggaaatg
     2521 ggaatatcgt taacagatgg tcgatttgag caaaacatgg atatgtttag tgaaagtact
     2581 agaaaagctt ttgaagagtg gaaaaatggc ggaggaacac aaaaagacgt tattaattcc
     2641 atgattcaag attttagcaa tatggatggt caatacgacc aattaaataa agcttctaca
     2701 atttggtctg cacttggcga agataacgcg atgaaagttg tccaatcttt gactgatgtt
     2761 aaccatacat ttgatgatgt tagtggatct gcacaaaaaa tgaatgaaga ttctactact
     2821 ccgttacaag aattaaacgg aaaaatagct gaattaaagg attcattagc tcctataggc
     2881 aacacaatca tagatgcact cgaaccagta attgattttc taggaaagat ggctgatgcg
     2941 tttaataatc ttccacaacc agtacaggat tatgccgtag cgattggcgg attgactgct
     3001 gcatttactt tattaatgcc aataatagtt ggcttcatgg ctctaggtgg tcctactaca
     3061 ttaataatag gagcagttat tactgttatt gctggagtta tagcaattat aaaaaactgg
     3121 ggcgcaatta ctgactggtt taagggaata tggagtaaat tcactgattg gttgggtggt
     3181 acttgggaaa gtataaaaga aggtgcctca tcagtttggg atggagttaa agaaacctgg
     3241 tctggatttg tagattgggt tcaagatatt tggcaaggag tttctgattg gtttggagag
     3301 ctatggagcg gattagttga aggagcttcc aacatctggc aaggagtcca agagacttgg
     3361 caagcattcg ttgattgggt ttcaaatatt tggaacggag tcaaagaagt atggtcgatt
     3421 atttgggcag acattgtagg aattgttcaa ataccatgga ccttaataac gtcattgatt
     3481 caagccggta ttaatattat cgtgggtatt tttgatgtag ctggacagtt attaggcgca
     3541 gcttggcaag ctgtttggac acctatttct gatttcctta aaaacacttg ggatactatg
     3601 acacaatggg taagcatcgc ttggaatgga attgtaacta cattccatac tatatttgat
     3661 ccagtagtgg catggtggaa tggtatatgg acagctatta gtactacggc ttcaaatatt
     3721 tggaattcaa ttagtgcaac agcttctagt atttggaaca gtatcaagaa tacaatcact
     3781 agcttggtac aagcagctgc tacagtaatt caaaatattt ggtcaactgt atctagttgg
     3841 ttaggtggaa tttggaattc aatcagctct acagcatcaa atatctggaa tagcgtgact
     3901 agtagtataa gcaatgctat aaacgcagct aaaagtgcca ttcaaagtgt ttggaatagt
     3961 atatcttcgt ggatcagcgg aatttggaac ggtatcaaaa acactgcttt gaatctttgg
     4021 aatggaatta caagcactat tagctctaaa gtaaacgatg gaaaaaatgc aatttcaagc
     4081 ggttggtcca atctaacagg tattgtttcc gacatattca ataatgttaa aagtacaatt
     4141 gctaacattt gggaaggtat caaaaagact gttagcgctc caattgattg gatcagagat
     4201 aaaatcagta gtatctttga taatttgaat atttctatac cacatattcc gttaccacat
     4261 tttaaattga gcggagaatt caatccattg aaggggaaaa tcccaacgtt gggtgttgat
     4321 tggtatgcga aaggtagtgt gtttaattct ccgaatatta tcggtgtcgg cgaagcagga
     4381 cctgaagcag ttttaccttt gaaaagatct gtgctgcaag aaattggtga tcgtatcttg
     4441 agtagcacat cagtttcatc tagggcacaa acgattcaac ctgtgaataa ctacgaattc
     4501 aatttcacaa ttgatggtaa cgcagatgag gttactatga agcaaacaac tcaacaaatc
     4561 attgatagca ttacaaaagt tcaaaatgat aatgcttcgg catggcgtta aacaggagag
     4621 tatttctcct gtttttttag tattaaaaag gatgtgaaaa aatgactgat tgtatacatt
     4681 ctataatcga tggatttcct gattatttgc ataaattggc tttagctgaa agaccaacca
     4741 taccttctcc aaaaagacag agagttgaaa cttctgtttt aggaaggtta ggtggcttag
     4801 tacaagatta ctcgtttgaa gacatgtcgt ttacattgca ctataactat ttagaggatg
     4861 tggaagacca tcaagcgttc aagcaatcgt tttatatcat gcgtcattgg ttaaattatg
     4921 caaagaaatt agaattctct gatgatccca acgtctatta tgttatccag actatcgata
     4981 ttggggatgc agaaaacgat attgttgaat ggggagagtt cgatgtaaat attactgcga
     5041 aaccattcgc aagagttcaa gaagatgtac ctataaccgt agataaacca cagtcattta
     5101 acttgctgaa taatagttta gaagaaagtt ttccaaagat tatcatcact ccttcagcta
     5161 cttcatgcca gttcatctta aatgattatg tgtttagttt tgaaggctta gtagtaggaa
     5221 ctgacgtagt cattgatagt gatttgatgc tttgctacga agagcaatcg gacggagata
     5281 ttttagatcg gtccaacaaa atgaagacca tgcaatatcc gacattgcaa gtggatatta
     5341 attattttaa ttgtactggt ttgagcaaaa tacaaattta tcgtaatggg ttaaggtagg
     5401 tgaaatagat gatcgataat ttaataacta tttacgataa aaacgacgcg aataatttag
     5461 ctgaacattt atatgatacg caaggtttag gcgctttgtc agactggtta acagctactg
     5521 ttagcaataa attaaacgga gccgagatat ttcagggtac ttatccaata agcggaacta
     5581 atgcagattt gattgtagaa ggacgtatta ttcagtgtta tgtagatgaa aatcgagcaa
     5641 aacagcgtct acggatttat tatgcaaaga cttctgtaat aggcaatacg atagaagtaa
     5701 aagctgaacc tattttcaat gatataagaa aatcggtgtt gaataaatat gacagtggaa
     5761 cagaaaagat cactgctagt caggcatggc aaaacgcaaa aactttagcg aaaccagtta
     5821 ttccttcaca gttttctttc tcgtcattag tagatacgct tgctaatgtg aagatagaaa
     5881 aggcgaattt tttagaattc tttggtggaa aagagggatc tattctagat cgatttcatg
     5941 gggaatttct aaaagataat aacacattac gtcatgaaaa aaggctaggc acggatcata
     6001 aaatcaaagc gatttatact aaaaacttaa ctggtcttga cttagagatt gatgctcaaa
     6061 gtgttttagt tggagtttat ccattcatta gcagctcttc agaaggagaa gacgagatca
     6121 ctctaccaga agaagttatt ttcacggatt acgtggatga ttatcctgct ggatatgttt
     6181 cttttgttga ttttaaagac aaagcgactg atgtagccac attaagggaa gctgctaaag
     6241 actggttgaa aacaaacata gataaacaaa aaccacaagt gagtggttcg attgaattag
     6301 taccattgag gcatcaaaga ggctatgaaa aatttgttga tctagaaaaa gtttcgatgg
     6361 gtgacggagt agatgtgtat catccacagt taaaagtgaa tatgtcagcg agaattgtgg
     6421 aatatacgtt taatgttcta accaattcat acgataaatt agttgtagga aacgtcaaaa
     6481 caaacttctt agaaaacaca gagaataatg tcagcaattt gattaatgat gccattgatc
     6541 aattgaaaaa cggtggcgaa atcagtgatt tactcaatga tattgtagat catcaaactg
     6601 atatgattac tggtcaaaat ggtggttatg ttttattaga tcctaaagaa gcgcctagtc
     6661 gtattttgat tatggacaca ccagataaga ataccgcaag gaatgtttta caaatcaaca
     6721 atgctggtat tggtttttct aaaactggca ttaatggaac atatgaaacg gcatggacgt
     6781 tagatggcgg attcaatgcc tcgtttatta cggctggtga aatagtagga attactatta
     6841 gaggtactac attaattagt gatggtgctg attatagaac aagtattgct aatggcaaaa
     6901 tgacttggta ctcaaaaaaa gttaacaaag atattatgga gctagaagca cgtgattatg
     6961 taagtgctga tgccggtatt gtatcataca ccatgaaaac tggtggtggt ttcatgatta
     7021 gaaatccaca gggtaacttg gtttttagta cgtgggataa tggtaataac agaccgtttc
     7081 tatcttttgg tgcgcccaat ttcaggtata gcaatgctag ttatgtaact tctggcgacg
     7141 gtagttcttt aagcattaat ggtagtgcgg gtaactcatg ggaatttaag gtagctggta
     7201 ggactatgaa atttactagt gatggtatgc taacgttacc aggttgtttt tttggttcat
     7261 gggaagatgg gaaacttgct aggtttgaac aatcaacggt acaagtatat aaagatttta
     7321 ctgttagagg tactaaaaac tcaactgtac caacagaaca ttatggacaa cgactattga
     7381 acgcttatga aactccagaa tattatttcg ctgattatgg ggaagccgtt acaggtgaca
     7441 atggtaaagt tcgtgttgat attgacccca tgtttgctga aacagtaaat ctaagtcggt
     7501 atatgacaca tgtgacacct acagaactag ttttgtgtgc tgttactcat gaagatattg
     7561 accatttcat cattgaaact agtaagccaa acgtattagt tagatggaat ttagtggcac
     7621 accgtctagg gtatgaagat attagattaa aagaggatac agcatatgat agcacagtgc
     7681 ttgaccaaaa acgtttttaa aacgaagaca aggaggtata taaatggcta gcagtttata
     7741 taatttggct ttagatttca gcaaagaatt aaactacacc aaagctatta tggctcgtca
     7801 aggtgataaa gggattacgg tgacggttaa accgtttcta aatggcttgc agatggatac
     7861 gagtggcgga acatttactt taaaaggaac aacaccatct aaccgttacg tagataatgt
     7921 tgcaactagt gtaactagtg aagaagtcac gttttctctt gatggcacat ttatgagtga
     7981 agcaggatat tataaacact gctacgtaga atatagaaaa gacaatcaaa ttttaacaac
     8041 gcaagatatc atttttttct cactaggagt gtctgacatt tcgcaaggcc aagccgatga
     8101 atatgtttcg caattagaag agttgattcg aaagtacaac gaaacttttg atgcttttat
     8161 ggctgaaatc aaaggtagag tggatagctt aaatcaacag attactgatt taactggtca
     8221 agctaaaacg ctacaagaca agttagatgc tctgaaagaa gaaatttcta agttaggtaa
     8281 cttacaagtg atgtacagta acagcatcga cttcgggggc tacgattata gcgggaatcc
     8341 gaatttaatg gctaatataa acgctgacag cttctcgcaa ggtagtggtg ccttatctgt
     8401 tgtagatgat ggtgacgagg tagtagtcac gcttgaccca aacaataaat tagagatatt
     8461 caaagcgaaa agtcaaccag ctctggtagt aggaaaaaca tataccatga gcgtagaaat
     8521 tatgctagaa ggcgatttca ctggagaccc tagcaagata ggcctaagat atattaaaat
     8581 gcctaactgg gtatcagggc tatatacgcg taatacatta actgctacta agggtgtatg
     8641 gcaaaaacta actggcaccg ttaaaattac tgctgcgagt gataacgctg aaagctggtt
     8701 tataatgcta caaaataaag acgctaataa cagcctatcc ggcaaactac gtttgagaca
     8761 cgctaaactc gaagaaggct caacagccac accatatcaa ccaaatttac tcgatgcgcc
     8821 gtattatttg agtaaggtgg ctttgggtga gaatattgct gatcctgcag ttagcttccc
     8881 aattaaaacg agcgcatacc gtttatacgg tgtgaacatg ctagaagagt ttaaagttgg
     8941 tcaaagatat acaattacaa taaaaggaac taaaccagca actcaagatt tttgggctta
     9001 caacggtggg aatatttcat tagagagaat gactccagta gaagggttag ttgacgtgtg
     9061 gacttgttca tttactatat taaaactaga tagtagttca ccaagtctat tgagtatata
     9121 tcaaacacca caatcaacag taggctcatg tcaaattgac tggctcaaga tcgaaaaagg
     9181 cgacacacga accccgaata ttgagcaata taaataccga ggaatcggca tgcgagactc
     9241 aaacaatcca aaagattatg tttgggatct agcaccagaa tatgtcgaag ataatttggc
     9301 cacagatatt aaaatttctg aaattactgg taaagcaaac aattataccg atgggaaagt
     9361 atcggagatt aattcgcagt tgactgcttc aattaatgaa gtagacacca cagctaagga
     9421 tgctcaaaca aaagcgaatg ctaatgcgac tgctatagat gaattagaca ataagatcga
     9481 tgaacgcatt aatgatacag ctactaccac attaacagtt acaaacggga ataccgga
//
LOCUS       DAHUVV010000099         9457 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.102, whole genome shotgun sequence.
ACCESSION   DAHUVV010000099 DAHUVV010000000
VERSION     DAHUVV010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.102"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            14..916
                     /gene="gnd"
                     /locus_tag="NK767_002363"
     CDS             14..916
                     /gene="gnd"
                     /locus_tag="NK767_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HCH6695318.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            918..1919
                     /locus_tag="NK767_002364"
     CDS             918..1919
                     /locus_tag="NK767_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HCH6695319.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1916..2200
                     /locus_tag="NK767_002365"
     CDS             1916..2200
                     /locus_tag="NK767_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCH6695320.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2233..3030
                     /locus_tag="NK767_002366"
     CDS             2233..3030
                     /locus_tag="NK767_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCH6695321.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3044..3877
                     /locus_tag="NK767_002367"
     CDS             3044..3877
                     /locus_tag="NK767_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6695322.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3895..4752
                     /locus_tag="NK767_002368"
     CDS             3895..4752
                     /locus_tag="NK767_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCH6695323.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4774..5283
                     /locus_tag="NK767_002369"
     CDS             4774..5283
                     /locus_tag="NK767_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6695324.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5320..5721
                     /locus_tag="NK767_002370"
     CDS             5320..5721
                     /locus_tag="NK767_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695325.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5833..6186
                     /locus_tag="NK767_002371"
     CDS             5833..6186
                     /locus_tag="NK767_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695326.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6220..6663
                     /locus_tag="NK767_002372"
     CDS             6220..6663
                     /locus_tag="NK767_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCH6695327.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6680..7765
                     /gene="uxuA"
                     /locus_tag="NK767_002373"
     CDS             6680..7765
                     /gene="uxuA"
                     /locus_tag="NK767_002373"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCH6695328.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7758..8471
                     /locus_tag="NK767_002374"
     CDS             7758..8471
                     /locus_tag="NK767_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCH6695329.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8678..8827)
                     /locus_tag="NK767_002375"
     CDS             complement(8678..8827)
                     /locus_tag="NK767_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695330.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9223..9423
                     /locus_tag="NK767_002376"
     CDS             9223..9423
                     /locus_tag="NK767_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCH6695331.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3172 a         1492 c         2103 g         2690 t
ORIGIN      
        1 tggaggcgct atcatggaaa ttggtgtaat tggtttggga aaaatgggat tgaatttagc
       61 tttaaactta aaagagcacc atcacaaagt acaaggactc gacatttcaa gtacagctgt
      121 tagtcaggca agagaaaatg acattcctgc ttatcaagag atctctgaat ttctagctac
      181 ttttgagcag aaaaaaatca tctggttaat gcttcctgct ggagagataa ccgaaaacat
      241 gttaagaaaa ctttttccaa aactcggacc aggagacatt attattgaag gcggaaactc
      301 ttattataaa gattcaatcc gtcgagcaga agagtttcag aaaaatgaaa tcggttactt
      361 ggattgtggg acttctggag gaatcgaagg agcacgacat caagcctgct taatgattgg
      421 aggagataaa gaaacttttc tatccgtgga acaattattc aaagatgtag caattgaaag
      481 aggatatctg tatgctggtg catccggaag cggacacttc ttgaaaatga tccataatgg
      541 aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc caacttgtac aaaaaagtga
      601 gtatgctttt gacttggaac aagtcgcacg tgtttggaat catggttcgg tgattcgcgg
      661 ctggctgatg gaaatcatgg aagatcagtt aaacgaacat cccaatttaa gtaattatcg
      721 gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt gaaacagcgc tagaaatggc
      781 tgttccagtt ccagtaattg caactagttt gttcatgagg aatctttcac aggaagaaga
      841 tagtttttca gcaaaagtag tttcagcact aagaaatggg tttggcggac atgaaatagt
      901 tgaaaagggt gagtaaaatg ccagattatc atatagcaat cgttaattcc agcagtttcg
      961 gaagagtctt tccacaccac ttggaaagat taaagtcagt tggagacgtt catttcttta
     1021 ctgttgatca agagattcca ggaaaagaac tagcggagtt acttaaagga ttcaatatca
     1081 ttattgcaag tgttacgcca ttttttcgac gagatttttt tgaaaacaaa gatgaactgc
     1141 tattgatttc ccgacatggt attggttaca acaatattga tttaaaagca gcaaaagaac
     1201 accagacaat cgtatctatc atacctgctt tagttgaacg aaatgctgta gcagaaaata
     1261 atataactaa tttgttgtcc atgcttcgac gcacgacaca agcacaagca agagtcaagg
     1321 acgatcgatg ggaagatcgg gcggagttcg tcggtaggac gctgtttaac aaaacagttg
     1381 gtgtgattgg tgcaggaaat accggaagcg gagtaatcga aattttgcgt aacggttttc
     1441 gctgtgatgt tctagcttat gatccttaca aatcagattt atacatcgaa agctttggtg
     1501 caaaaaaagt agaacttcaa gaattactgg aacaatcaga tatcatttgt ttatgtgcga
     1561 atttgacaga agagaactat cacatgattt ctgataaaga agtcgatcag atgaaagaaa
     1621 atgtttatct atctaatagt gcaagaggcg ctctagttga tgaagaagcg atgattaatg
     1681 gtctgaaaag cggaaagatc gcaggatatg ccacagatgt tttagaagaa gaaccgggac
     1741 gtcaagatca tccgtattta gcgtttgaac aagtagtgat tacgccacat atatcggcct
     1801 acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt cagagatgtc gaacagatca
     1861 gtcagcacct tcttcctgaa cgttctgtcc agacaaagag caactatata aaaggatgat
     1921 gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct caattttatt tatatgaaga
     1981 aggtggtatt tcaaaagttt ctgatctctt gaaatcatac ggcgcgaaaa gagtactggt
     2041 cacccacgga acggtatcat gggaaaaggc gctacctaaa ttagtatttt tgaatgacga
     2101 aacgattcaa tttttttatc atcgttattc tggagaatgt agttatgctg aagccagacg
     2161 aatagctaca atcatcaaaa aaatgaaatc gatttcgtag ttggcgttgg cggaggaaaa
     2221 ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa tcgatttcgg agtcatccct
     2281 actttagcta gtaattgtgc tccatggaca cctcttgctg tcatgtataa ggagaatgga
     2341 caagcagaag ggaaaacaga acacttttac agacaagctg ctttttttat aacagatccc
     2401 caattagtga ttgattcccc tgttccatat ttcattgccg ggttagcaga tacactagca
     2461 aaatggtatg aatctgagct catcttagaa caagattttt tgcaagatga atcatttttg
     2521 aagctagcac aagatactgc gaagatctgc aaagaagaga ttttgaatca ttctgctaaa
     2581 gcaatccaag atatggcaca aagaaaactg acatcggagt tcaaacagct ttcagagatt
     2641 atctttgctg ttgctggttt agttggagga ttaggagata aatacgcaag aaatgtggca
     2701 gcacatgcta tgcatgacgc tatcagtaaa tatattccgg aaagccatcg ttttctacat
     2761 ggagaaaaag tagcgtatgg gattttctat caactggcac tagaaggtcg ctgggaaaaa
     2821 atagacgagt tatatccttt gtatcgggaa ttagaactgc cggtttctct agaacaaatg
     2881 ggattatttc cgaaagataa acagataata gataaaatgg ctgaatttat tgattcgaaa
     2941 gagaaagtgc atttgattcc tatagaggta acacagaaat cactgataca gtcaatgatg
     3001 gaactggaag gttatatcag aaagatctga aggaggagga cacatggaaa cattaagtgt
     3061 agtacaaagt ttgttgattg ctttatgggt agccgcggtt atgtcccgct ggttaggtgg
     3121 aggagccacg ttgaccttac gtttttctcc tttgatgact ggattagtcg tcgggttgat
     3181 catgggggaa gttgcacaag cgatgattgt tacagccgct ttgcaaatga tttatatggg
     3241 ggttttttct ccaggaggat caatgccagc tgaaccatct attgcagcag ctatcgcagt
     3301 accagtcgca ttattagggg atctgtcgcc agaagcagcg attgccgttg cggtgccagt
     3361 cggattgctc ggaagttact tgtatcagtt tcgttttttc atcaatactt ttttaggtaa
     3421 atatacagac aaagctgtag aagatttgaa tacaaaagct atggcccgat caattattct
     3481 ttatccaacg gttgcctcat ttttattatt cgttccgctt gttttcattg ctttatattt
     3541 aggggcgcca ttgatcgctg atgtaatcaa agctttagag ggaacagtcg ttatccacat
     3601 tcttgaagta gtaggtggag ggttagcggc aatcggtatc gcgacaactg tatacgttat
     3661 cggtcgaaaa gattatctgg tctttttctt ccttgcttat tttatgagtg ttgttttgaa
     3721 atctttagat attacgatgg ttacttatgc catttttggg attattatcg ctttgatctt
     3781 tgtccagtcg caaaaaggga aacaagtaca gcctgccaca agcaataaca attcagcatc
     3841 ttttgatgac gaagatgact atgatgacgg attttaattt aggaggaatg ggaaatggct
     3901 gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga tgtaacaaaa
     3961 gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg ttacttggcg
     4021 ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga tgaagactct
     4081 ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag ctggggtggc
     4141 ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga agagtatgaa
     4201 gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg attgatgggg
     4261 gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat ttttatcgca
     4321 atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc ttttatctgc
     4381 tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt cactatgggc
     4441 cgtaatgcta caggcctaaa gcaaaacact ggtgttcaga cagcgattga aatgctatca
     4501 gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt agaatcttca
     4561 ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga tcaaatcgtg
     4621 ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac gaaaaaagga
     4681 ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct agcaggaatc
     4741 ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt agcgcgtgtg
     4801 gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag tggagtgaat
     4861 tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga tctttacaaa
     4921 aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa agctaaattc
     4981 tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa aacagtccaa
     5041 acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat tggagggatt
     5101 gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt aaataaatca
     5161 gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat ttattttcaa
     5221 gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt tgatttgtca
     5281 taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa cgagctgttt
     5341 cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa aaagagtaaa
     5401 cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga tgtgacagtc
     5461 attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga tatcgaaaaa
     5521 gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg aagttatttc
     5581 atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt gtccttcatt
     5641 ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc tcatcacgta
     5701 ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag ctactttttc
     5761 ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg aaatacttca
     5821 tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt agcgtatgcg
     5881 tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc gggtatgaaa
     5941 aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct acagttttcg
     6001 ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt gaaagaaaaa
     6061 aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta tttgaccaaa
     6121 acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat tgctcttttt
     6181 cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat catgatcgtt
     6241 acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt cgggacatcg
     6301 agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt cggagagaag
     6361 gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt tgttgatcta
     6421 attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac tgaagaaaag
     6481 aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga agcaatcaat
     6541 catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa acaaggaaaa
     6601 gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga ggacgatttt
     6661 tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa aaggggattc
     6721 gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg gaactttgct
     6781 gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga aagcatctgt
     6841 tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg atgcaatcaa
     6901 ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa tcaaaaactt
     6961 gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg gatgggcgaa
     7021 aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg atcagaaaat
     7081 cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt caaagggatt
     7141 caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg aaaaactgta
     7201 tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag aaaaagtgat
     7261 tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc cgccatggga
     7321 actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa ttctaaatgc
     7381 agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg caaatccgga
     7441 aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg tccacttcag
     7501 aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct ctacagaagg
     7561 ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg acggtcctat
     7621 acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat atggcctgta
     7681 tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa gtaaggaaaa
     7741 accactggga gagcattatg aataaaatca agctcttcat ctttgatatg gatggccttt
     7801 tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa aagtatgatt
     7861 atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg gcgataagag
     7921 aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga gaagcaatca
     7981 atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa aaaggagcag
     8041 aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct tcgtctaata
     8101 cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg tttgatcaaa
     8161 tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt ttgaaagctt
     8221 gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg attgcaggga
     8281 tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat ttgatagggt
     8341 tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat gaatccaata
     8401 aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa agtaaagggt
     8461 tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc tgcacgactg
     8521 agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta tttgtagtga
     8581 acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg cgtttaaaga
     8641 gaagaacgag aatcgtccat atgagggttt ccactcctta tgcagatttt tttaattctt
     8701 gcactgcttg ttcgatcgta acacctgtcg ctacatgatt ggtattttct ttgtcaaaaa
     8761 cgacaaatag attcaaatct gtatccaagg ttactcgata ttgtatatct ttgttataga
     8821 atgtcatagc cattctcctt tctttaaata cttgctgaaa ggcaacgaac gcgttgataa
     8881 cgaaattcca tttataagta taccaccaat tgaaaggttt tcatagttaa gaaattttaa
     8941 agtcaaaagt tgcagataaa agcaaataaa caagaaaaca aaaagtgaat caatcgcggt
     9001 tattcaactt tttcttcgat tcgaagacaa tagaagacaa tgaattttat atttcattgc
     9061 attatccttg tgttattaga aaaagcatga tatattaaat gagtaatcta ttttgaaacg
     9121 taatttgagc gatatatcta catagataaa actccttttg cctatcaaat atttattgca
     9181 acgaaaaaac gtacattgat acgtatcagg aggaatttat ttatgaataa cggtacagta
     9241 aaatggttca acgcagacaa aggttttggc tttatcactg gtgaagatgg aaatgatgta
     9301 tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga aggccaagca
     9361 gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag caacaaacat tgttaaagct
     9421 taataaaaat tgacttttaa ccgtcttttc gaaagaa
//
LOCUS       DAHUVV010000100         9183 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.103, whole genome shotgun sequence.
ACCESSION   DAHUVV010000100 DAHUVV010000000
VERSION     DAHUVV010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.103"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            61..2838
                     /locus_tag="NK767_002377"
     CDS             61..2838
                     /locus_tag="NK767_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCH6695332.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            2919..3338
                     /locus_tag="NK767_002378"
     CDS             2919..3338
                     /locus_tag="NK767_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HCH6695333.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            3350..3820
                     /locus_tag="NK767_002379"
     CDS             3350..3820
                     /locus_tag="NK767_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6695334.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            3837..4616
                     /locus_tag="NK767_002380"
     CDS             3837..4616
                     /locus_tag="NK767_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6695335.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            4603..5424
                     /locus_tag="NK767_002381"
     CDS             4603..5424
                     /locus_tag="NK767_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCH6695336.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            5441..6490
                     /locus_tag="NK767_002382"
     CDS             5441..6490
                     /locus_tag="NK767_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCH6695337.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            6503..7507
                     /locus_tag="NK767_002383"
     CDS             6503..7507
                     /locus_tag="NK767_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCH6695338.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8140..9159
                     /locus_tag="NK767_002384"
     CDS             8140..9159
                     /locus_tag="NK767_002384"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCH6695339.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3058 a         1540 c         1942 g         2643 t
ORIGIN      
        1 tcaattcttt attaaagatt ttattgacaa aatgaatccg ttttctatga tggaggaggg
       61 gtgaataagt tgttgaagaa ggaaatttta tgttacttag agaaccagac agcctttttc
      121 gatttagaac atatgaacga ggtctttaca gcacaaaggt tagctgatca ttttggggta
      181 aaaagaaaca cagtcagtca ttatttgaac cagttaaatg atgagcaact actagtcaaa
      241 atcaattctc gaccagtgat ttatttccac aaagaagctt ttgaaaaaca attttttaag
      301 ttacgcacga atttttattt ttctatcgat gatctcaaag aggaacagcc ttttttcgcg
      361 caaccaaaag acttattctc ttttatgatc ggatacgacg ccagtttgaa agaaagtatc
      421 gaacaaatca aaacagctct ttactatcca gatggcggat taccactttt gattacaggc
      481 gaaagtggca caggtaaaag ttatttagtt aatttggtct atcagtattg tctgcttcat
      541 gatctgctag aagattctgc accattcatc acttttaatt gtgcacaata tgcagataat
      601 cctgaacttt tgactagcaa tttgtttgga catgttaagg gagcgtacac tggtgcagaa
      661 gaaaataaaa aaggggcttt tgaagcagca gatggtggga tcttgttttt agacgaggta
      721 catcgcttaa caccagaagg tcaggaaaaa ctgttcacct atcttgatca aggtgtgatt
      781 tatcgtatgg gagaacctaa tttgtcacgc cggatcaaaa cacgtctctt ttttgctacg
      841 actgaagaga ttactagtca tttcttaaca actttcattc gaagaatacc tatccgaatc
      901 gaactacctg cacttaatca gcgtagtcgt aatgaacggc tagaactgat ttactctttt
      961 tttctcgaag aacaaagaaa aatggggagc cctttgtcag tcagtggacc agctttgtca
     1021 cttttaacaa gtcagaaatt atccggaaac ataggagagc tgaaaaatat cgtcaaagtt
     1081 tcagcagcaa aagcatatgc tgaacagcgt gaacagcctg aaatccatgt cacgatccat
     1141 catttgcaaa aagaactact tgcgcagcct attactaaaa atacagcaca gcaaggaatc
     1201 tatattacgc aagaccagac cttagagcaa ttaatggaga agcaacagcc cgaacagcag
     1261 cgaatcgtta aaagttttga gcaaatccta attgatttca agaaaaataa ctgtctgcta
     1321 tattcttgcg aaggaaaatt aaagcaagaa gtgattcaat tatttgattt tttgctgttt
     1381 gagaccagta gacaagaaaa acacgagctg ctggtttatt ggacacaatc tatccgagag
     1441 acctttcgac aaatggaaac tgcctatcaa atcaaattcg atggaaacag tgtctatgcc
     1501 ttgagttact atctctatca gcgaagcact atcaaatggt taccggaaga acatgatatt
     1561 atccaactaa tcaaagaatt agaacaacaa gtttctcagt cttatccatc tagttatcac
     1621 tatgtccaac gaattctgga attaagtaag atcaaattag atattgaaga cacaagtatg
     1681 gatcggattt tactgacgat ctatctaaaa aaagcgaaat ggtcaaaaga aaaacgactg
     1741 ccaaaagcga tgattgctgc tcacggttat gcaactgcta gcagtatggc aaatgtagta
     1801 aatcgtttat tacaagagga tctatttgaa tcatttgata tgccactaga tgtcacacca
     1861 cagcagattg cttcagaaat ccttgattat tgcgaaaaca gcgatgtttc aaatggattg
     1921 atcatattag tagacatggg atctctgaaa gaaatccatc agttttttaa aaaacagtta
     1981 tccgtaccgc ttttgatttt gaataatgtg acgacacctt tagcaatcac agtaggggaa
     2041 tgcctgcaaa aaaatgtatc attagaagag atagcagaag agactgttcg acagatccag
     2101 ccagaatggc gactgctcta tccagaggaa aacaaaccca aagcactcat tactacttgc
     2161 ttcactggaa tcggtacggc ggtccactta agtgaactgc tagaaaaaag cttacctacg
     2221 gcttgtcagt tgaaaatcat cccatatgaa tatcagcaat taaaagataa gaaaaacagt
     2281 gacccattat tttccatata cgaagtcgta ggaatgattg gcaccacaga tcctgatatc
     2341 accaatatcg actatctttc attagaagac ctcatctcgg gggaaaagat gtctgtacta
     2401 gctgaatggt tggacactac tatgaatgct tcagagaagg aaatattcaa tcagcgcatc
     2461 attcgtaatt tttcattgga aaaagtactg gattccgtga ctattctaga tacagagaag
     2521 gtgatgggag aaatcgatca ttttatgaga gaattagagg tgcagttgaa tcgaagcatc
     2581 agcaatccca aaaaattagc gttgtatgtc catgtgagct gtctcattga acgattgatc
     2641 cgacaaatgc caatcgaaac ctatcaagga ttagagaaat ggaagcagtg tcaaaaagaa
     2701 gggttatctg ctattaaatc tgcgtttagt gtcattgaag agcattatag tgtcatgatc
     2761 cccaattctg agattgccta tatctatgat attttgagtg aagatacaga gtttctctcg
     2821 atagaagaag aattttgaac ataagcaagt tgttgccact aattttggca tgcaacttgc
     2881 ttttttagta tgtgtaagtg aaaagaggag atgaaaaagt ggatagagaa attatcttag
     2941 cttcccatgg aaaatttgct tcagggatat tagattcatt agagctgatc tatgggaaaa
     3001 atcattctat tactgtattc gattgctata ctgatgagaa ttttgattta gcggaaacag
     3061 taaggcagct ttttatgagg tacaaagata aagaaatcat tgttctgacg gatctttttg
     3121 gcggaagtgt caacaacgaa ttcttacaat atatcaatcg ggatcatgtc tatctcgttg
     3181 ccggattgaa tttgccacta ttgattgagc tagtatctcg gttggacgct gaagtagaga
     3241 caactgtttt gatccagcag gtgctaactg attcaaaaaa aatgatccag ttttgtaacg
     3301 agcggctcaa tcagaaaata gaagaagacg aattttagga ggaagaaaaa tgattgtatt
     3361 gacaagagtc gaccatcggt tattacacgg acaagtagct ttttcatgga cacaaacaat
     3421 aggcgcagac tgtattttga ttgctaatga tgatgtacct acgaatgaga tacgaaagac
     3481 aacgatcaaa ttagccaagc cacaaggagt caaactagtg atcaaatcaa ttgatgactc
     3541 gattgccgcg ttgaaaagcg gggtgacaga taaatataag ttattcatcg tagtggaatc
     3601 gatcgaggat gcatataagc tggcagaagc gtatccagaa atcaaaacca tcaacttagg
     3661 agggatcaag gcaaaacctg gtactcgcag catcggaaaa gcggtaaata ttttaccaga
     3721 agaggaggaa cgcttgaaac gattaattga caaacaaata gaagtagaaa tccgtcaagt
     3781 accagcagat aaaaaagtgg atgtaagaga agttttgtaa gaaaggaaga acctcaatga
     3841 ttgtacaagc agtcttatta ggaattgttg catttgtcgc acaatcagaa tatgcactag
     3901 ggacatcgtt gctttctcgt ccgattgtga cggggctgtt tacaggaatc gtgttaggtg
     3961 atgtaaaaac tgggatcatt atgggcgcaa ctttagagtt agcatttatc ggatcgttct
     4021 cagtaggtgc ttcgattcca cctgatgttg tgaccggtgg gattttaggg acggcttttg
     4081 ctattacagc tggagccggt acagaaaccg cgcttttact aggtttgccg attgctacgt
     4141 tgacattgat attgaaaaat atttacttag ggcttttgat tccaattatg aaccataaag
     4201 cagacaatta tgcagaagaa ggaaattata aaggagtcga gcatatgcac ttgcttgccg
     4261 gtatcggtct ttccttgatg cttggtgttg tggtaatggt ttcttatatg gtgggaagca
     4321 atacgatcgg aaatctatta aatatgattc ctgacttcgt tcaaaagggg ttgtcagtag
     4381 ctacagggtt gattccagct cttggttttg cgatgcttgc tcgtttgctg atcaacaaac
     4441 aagttgcacc atattttttc ttaggttttg ctatagcttc ttacttagag attcctgtga
     4501 ccggagtagc tatttttggt gcgattttag cagttgtggt agtcaatatt atgaatgtcc
     4561 gtcaactacc acttcaaaca actgcagggg aggtagaaga agatgaagaa ttctgagatc
     4621 aaaaacagca aaataacgaa aaaagagcta aatcacgtat tttggcgttc ttttcaaatg
     4681 gagttttctt ggaattatga acgtcagatg aatatggctt atgtttatgc aatgattccg
     4741 attttgaaaa agttatatca gtcgaaagaa gaaatggcag cagctttgaa acggcatcta
     4801 gagtttttta acacgacccc acatatcgtt acactgattt taggaatcac ggctgcaatg
     4861 gaggaagaaa ataaggatga tcctgaattt gatgtgactg cgatcgatag tatcaaaaca
     4921 tctttgatgg gacctcttgc agggttagga gattctttct tttggggaac tttgcgattg
     4981 attgccacag gtgtgggtac ctctttagcc atgcaaggaa atattttagg ccctatcttg
     5041 ttcattttga ttttcaatat tccgcacatt ctgtttcgct atctagcaac aggttgggga
     5101 tatcgcttag gaacgggttt tttgaaaaag atccaggcaa acggtatgat ggaaagtttg
     5161 acattaggcg cgtctattat tggactgatg gtcgttggag caatgactgc cacgatgatc
     5221 accatcaata ttcctttaaa aatcggttca ggagaaaatg ctgtgactgt tcaaagtatt
     5281 tttgatgata tcgttccaaa tatcttatgt cttggagcat ttggcatagt gttttatcta
     5341 ttgaagaaag aagtgaaacc attaacgatc ttgatcggac tagctttctt tggaattttt
     5401 ggttcgtgga tagggatttt ttaaaaaagg agcagagaaa atgtttacga tgcaagatta
     5461 tatctatgaa gaaaaggaag tactaagtac gatcttgaaa aagaatgatt tttcgacaag
     5521 agaacatatg aaaaaaacag tgaatttact gatcttagca acgggttcat cttataacgc
     5581 ttgtttagca gcgaagccag cattggaatc ttatggtgat cttactgtcg atattcaaga
     5641 acctttttat tttttcaatt atggaaaact ttctccttca atcgatactg tgattgctgt
     5701 gtcacaaagc gggaaaagtg cttctactgt tgaagcagta aaaatgatac aaaaacaagg
     5761 gttacctgtt gttgctatca caaatgacgt gcaaagtcca cttgctttag aagctgcaca
     5821 aattattgat ttaggtgccg gggtagaatc agtaggcttc gttacaaaag gctattctgc
     5881 gactgtttta cagttactac tcttagggat aggaattgga ataagtaaaa ataaaatcag
     5941 caaaaaaata gaacaagact acatgcaaca gttgcgcaaa ataatcaacc atttaccagc
     6001 gattattcaa aaaacggaag aattttttga tggatatcaa agtttatttc gtttagcaca
     6061 gcgtttcgtc accattggat atggtccaaa ctggggaaca gctaaagaag cagagacgaa
     6121 attgactgaa acaatccgcg ttccgtcaca aggatttgag ttagaagctt atatgcacgg
     6181 cccatacctg gaggcagatg cttcgcattt gctattcttt attgaagggg aaagtgtaaa
     6241 taaggaacgt tcgcaaaagt tgcaaagata tatgagtcgg tacgttggag agactctcac
     6301 cattaccaca aaaaaagcaa gaaacgagaa aacacttgga ttagcaatag aatgtgatga
     6361 atatctgtct gtattagcgc ttgtcgttcc gtttcaacta ttcgcttata aaaccgcagt
     6421 agctaaaggt attgacttga acaaaaagat ttttgaagac tttgatacag tcttgaaaag
     6481 taaattataa gaggtggaaa tcatgctgaa attcaatgaa gaagaacaaa tcaaagcaat
     6541 aaaaggcgca ctagctttac gtccgcaagt agaagaaatc atcgataaac tttatgcgga
     6601 aaaatttgac gctgtctatt atctaggaat cggtggaacg tatgcttcct cgatgcaagc
     6661 tgtcacttat atgaatggga aaagcaactt gcctgtcttt gtacagcatg ctgcagaata
     6721 ttacactact ggaaacaaaa gattaacaaa agattctatc gttattttgt cctctgttac
     6781 cggtacaact caagaagtag tgaaagcagt agaacaaatc aagaaagtag gagcgacact
     6841 gattggattt atcgataaag cgaacagtaa gttgtctcag ctatgcgatt ttgtggtcac
     6901 ttatccagct cctggaacag aacaaatcaa attttttatg gcagcagatc gattgatgta
     6961 tttgaatgga gaattcgaag cttattcaga atactatgaa cagctcgaac gttatttacc
     7021 aacagggtta gtggaagcag agaaaaaagc agatgcattt ggcttatcat ttgcagaaaa
     7081 acatcgacgt gatagtatgc attattttat tggagcaggg aatcaatggg gagctgttta
     7141 ttcatatgcc atgtgttact gggaagaaca aagctggctt ccatcgaaat ctatccatgc
     7201 tgccgagttt cttcacggga cgttagagat cgtagaagaa acaaccccag tcacattatt
     7261 tttaggcgaa gatgaacaga gagtgttatc tgaacgtgtg gctaaacttt tgccaaaaat
     7321 ttgcagcaat tatacattga tagacacaaa agactatcca gtagaaggta tcagtgaaaa
     7381 atatcgcgga cgtgttttgt ctttcttatt gatgcatgta gtgacacaac gcatcgatgc
     7441 tcatgtggag cagttgaact gccatccatt agagatacgc cgctactatc gtcagtttga
     7501 ttattaggat agactggata aaaagaatat aaaaaataaa gagagcagcg ataactgctc
     7561 cctttatttt tttaaaatat tggcatcaat tacgttataa aacacatttt ttgcattatc
     7621 tacagttcaa cttataatct gtttaatggg acatgtgggt gccaaccatt ttttgttatg
     7681 caatagtact atttagttgt tgcatggatc cgtagtaaaa gtccaagtac aacgagttgc
     7741 gccagtttac atagcgtttt gtatccagaa tgttgactaa aacaaaagtt cctatgtcaa
     7801 ccgtcaacag agacaatctg tccgttgcag gacctgacag atttagaaat gtccaatctg
     7861 tctcttatgt attttaaatc aatactcact aggagaagtt tcctaacctc ctaatagtaa
     7921 tgctggtagt aacattgtca aaactgcttc ctcttaggaa caagcaaagc ataacgagtg
     7981 taaaaataaa atgtagacta ttgtaacata tcattgatta tttcaagtaa aaacgataaa
     8041 agaagtaaac aaacacttaa aaacaatgga aattagtccc aactttaaga aatgtatact
     8101 ataatggaaa ttatcaatta tttatttgga ggaatatcca tgaaacaaaa aatcgcagtt
     8161 cttggtcctg gatcttgggg cactgcttta gcacaaacat tggcagaaaa cgggcatgat
     8221 gtccgtattt ggggaaatgt acctgaacag attgatgaaa tcaataagta tcatacaaat
     8281 cagcattttt tacctgattt gacgatccca gaatcgatta tcggatataa agaattggca
     8341 gaagcagtgg ataacgcaga tgctatttta tttgttgttc caacaaaagc gattcgttca
     8401 gtcgcacagg aacttattgg taaaatgaat actaagccaa tcattataca tgcaagtaaa
     8461 ggactagaac aagatacgca taaacgaatc tcagaagttt tggctgaaga aattccagaa
     8521 gaaaaaagac aggcaattgt tgtattatct ggtcctagcc atgctgagga agtggcagta
     8581 catgatatca caacaattac agctgcaagt attgatcaaa aagcagcagc ctatgtacaa
     8641 aaactgttca tgaatgatta tttcaggatt tatacaaata atgatgtgat tggcgtagaa
     8701 actggtgcag cactgaaaaa tatcattgca attggtgccg gcgctatcca tggattagga
     8761 ttcggagatg atgcaaaagc agcaatcatg actcgaggat tggcagaaat cagccgtctt
     8821 ggtgtcgcga tgggtgccaa tcctctgaca tttatcggtt tgagtggtgt gggggacttg
     8881 atcgttactt gtacaagtgt tcattctcgc aattggcgtg ccggtaactt gttaggacag
     8941 ggacataagc tggaagaagt tctagaaaac atgggaatgg tcgttgaggg tgtggcaacg
     9001 acaaaagcag cagtagagct agctcaacag ctaggtgttg agatgccaat tactcaaaca
     9061 atctataatg ttttgtataa tggagaagat atcaaaaaag cagcaaaaga aatcatgtta
     9121 cgtgacggaa aaatggagaa tgaattttct cttcgttaaa tagatgatca atagttaatc
     9181 gtc
//
LOCUS       DAHUVV010000101         9122 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.104, whole genome shotgun sequence.
ACCESSION   DAHUVV010000101 DAHUVV010000000
VERSION     DAHUVV010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.104"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(193..411)
                     /locus_tag="NK767_002385"
                     /pseudo
     CDS             complement(193..411)
                     /locus_tag="NK767_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            652..834
                     /locus_tag="NK767_002386"
     CDS             652..834
                     /locus_tag="NK767_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695340.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            818..1003
                     /locus_tag="NK767_002387"
     CDS             818..1003
                     /locus_tag="NK767_002387"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCH6695341.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            1246..2766
                     /locus_tag="NK767_002388"
     CDS             1246..2766
                     /locus_tag="NK767_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCH6695342.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(2953..3525)
                     /locus_tag="NK767_002389"
     CDS             complement(2953..3525)
                     /locus_tag="NK767_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCH6695343.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(3532..4194)
                     /gene="rpe"
                     /locus_tag="NK767_002390"
     CDS             complement(3532..4194)
                     /gene="rpe"
                     /locus_tag="NK767_002390"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCH6695344.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(4210..4725)
                     /locus_tag="NK767_002391"
     CDS             complement(4210..4725)
                     /locus_tag="NK767_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCH6695345.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(4727..5011)
                     /locus_tag="NK767_002392"
     CDS             complement(4727..5011)
                     /locus_tag="NK767_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6695346.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(5045..6373)
                     /locus_tag="NK767_002393"
     CDS             complement(5045..6373)
                     /locus_tag="NK767_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCH6695347.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(6387..6842)
                     /locus_tag="NK767_002394"
     CDS             complement(6387..6842)
                     /locus_tag="NK767_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCH6695348.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(6845..8791)
                     /locus_tag="NK767_002395"
     CDS             complement(6845..8791)
                     /locus_tag="NK767_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCH6695349.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         3014 a         1687 c         1440 g         2981 t
ORIGIN      
        1 ttttaccaaa tgataagctt taaaaagtat tcagaattta gtcatgttac ggatccaata
       61 aagtagatat ggaatattta ttttctcttt tctaatttat aagaactaaa tgatttcatc
      121 attattttga cacttttagt tgggagcgtc aataattttg tgtcaatggt tcttccctac
      181 tgtagattgt ttctaattct ccatcggttg actcagcatt tgttcgagct cgccttcggc
      241 ctgttggaaa cctttatgac tgcgacctag gaatttttgg ttgtaagtat caaaagacga
      301 gactaagaaa cgttccatcg actcttcatt ttggaactgt tccttgcggt ggctgtattt
      361 cttgatttgt ttattgaaag attcgattaa attagttgag taaatgcttc gtacgaacat
      421 agttgattta ttcaccatac ttcccattac atcgtaaaac gctatattta agatttcaaa
      481 caactatatt aataggaaga aaacttcatt tttcctctta gttctataaa aagtgtggct
      541 aatttgaggc aaaatattaa attaaatgta agcgtgtcat aggatagtat ctcgtacgcc
      601 gattatcttt cttctataat taaactatca aatatagaag gagaaaaacg aatgaataaa
      661 aaagatattg taccagttca attttcaagt gcggctcaaa aatcgcaaat tgacaccaag
      721 ccaaaactag cttgcagaat caaatcggaa gataaggaaa tatttattta taatggaatg
      781 gataaatatc ttttgtatac cttgttaaaa gagttgaatg ccaatgctca ttgatgcaac
      841 tgcattgaaa catattttta tcgtttgtgg aaaaacagat cttcgaaaag gaattgatgg
      901 actagcagct ttcgttatag aagaatatga ccttgatgtt tacgatcaag cgcttttctt
      961 attttgcggt acgaagaaag atcgcttcaa ggctctttac taggatcaaa atggatttgt
     1021 attaatgtac aaaagatttg aaagtgggta ccttcaatgg ccgcgttcca gaaaagaagt
     1081 cttactgact ggcctttcaa tcgaagaaaa atgtacgcta aaagaaacga aaaaaggtgc
     1141 gatatattag acgaaaacct tgttaaatca aggattttgt ctgtttttcg ttgtcataaa
     1201 atggtataat agatgtaaga aaatcgctag aatggtggtg taaaaatgac aaaaatggaa
     1261 tctgaacttc ttgtacaaat caagcaatta acacagttgc ttgaaatcca gcaacaggaa
     1321 aatactcttc ttagagaaca aattgctgaa atgaatcgac gcttgtttgg acgaaaaaaa
     1381 gaaacaccac ccgtagatgg gcaaatcgat ttattagacg actcaacttt taatgaacca
     1441 gagcacactg gacaagaaag ccaggaaccc attacggtat cttcttttaa acgcagaaaa
     1501 cgcaagggac tcaaagcact ttcattagaa ggtctgcctg aagttcctat tcattatgaa
     1561 ttaaaaggtg ccgattgttt gtgtgaagac tgtggtcata ctttacatga tatcggtgcg
     1621 acaaggctta gagaagagcc attgtatgtt cctgcccata tagaaaaaca agtcatctac
     1681 caacatgcct atcattgcag agaatgtgaa gcattaggag aaacaaaaat caaaaaagcg
     1741 cctgttccta aaccgttgat caataatagt ctaggttcct cttcaattgt gacacagagt
     1801 atttgggaaa aatttgttaa aaaggtgccg gcatatcgac aagaaaaaga atggcatgca
     1861 cttggtttcc ctcttgatcg taaaaaaatc actaattggc atatcaaggt aagtgaaatg
     1921 gttttagagc caatctacaa ttgccttcat caagaattaa gcaatcagga ggtcttacac
     1981 gcggatgaga caagctatcg ggtattgaat actcataaag aaaagacctt ttactggtta
     2041 tatgcttcag gtaaaagcga agaaaaacaa gttgtgctct acgagcacgc aaattcgcgt
     2101 tccgttgatg agccaaaaag ttttttagaa tcatttagcg gtttcttgca tactgatggt
     2161 tacgcggcct atggaaagct gccggtaaac aatgtttatt gttgggcgca tgttcgcaga
     2221 aagttttttg aagcattggg taaacaaccg tccccaaaaa gtcatgccaa aataggatta
     2281 gacttctgtg acaagatgtt tcgaatcgaa cggaccttta ataagcttag cgcagaagaa
     2341 aggtttgtag cgaggaagga aacgttgcag cctgtgatga ctgaattttt tcattggtgt
     2401 gagagttttg cttatttgcc aaaatcgcag cttggaaagg cggttcaata cgcagtcaat
     2461 cataaagacg gacttcagat tgtgttgctt gatggacgct tggaactttc aaataaccga
     2521 gcagagcgtg ccatcaaaga gttagtgatc ggtcgaaaaa actggctatt ttctaaaagc
     2581 ttaaaaggcg ctagatcaaa tggaattata ttgagtatta tccagacagc tgttgcgaat
     2641 gggttaaaca tccgcaaata tttaaatcat ctttttacag agattcccaa tctgtcttcg
     2701 atgacacctg aagcgctacg cgcttatttg ccatggaatc agcagataca agaaatctgt
     2761 aaataatata agccacaaat aacctgattt gagtgtatca aatcgggcta tttgtgttta
     2821 ggtaccattc tatgacacgc ttacgctcaa aactactgaa ttattaagtt atgtctaatc
     2881 aaataaaaaa accattctca tatttgctga ataattagca tttagagaat ggttttttat
     2941 ctttaaaatc tactactcaa aatctgcatg tcgagcaaac atcgtttctg agctttcacc
     3001 tgttttatcc attaaagata acactaaact atcaaaggtg agaaatgcta attgttcaaa
     3061 acttgaaccc ataggttgtg aaaaagtatc ttcttctcgt tgattttcat ctttggtaat
     3121 acctggtaat acaagaacag aatctgctaa ttgaccaatc gacgagtctt ttttcatggt
     3181 aactaaagca atgcttacac cactttgatt tgctttggcc gctaaatttt ttaaactacc
     3241 tgtttcacct gagccagaac agataattaa taaatctcca ggttgcgaat gtggtgaact
     3301 aatttcaccg acaaggctga ctgaaaaacc taaatgcatt aagcgattcg taaatgcttg
     3361 catggcaatt ccagagcgtc ctgcaccagc tagaaagatg tgtttagctt gaacaatttt
     3421 ctcactaaat tgttctaact ctacagaatt aatacgtttt gagttatttt gtaattctaa
     3481 gataatcgct tctaaatagt ttttattttg gtctttattg gtcatgatct tttacttcac
     3541 ttcctctaat gattgattta gtttatcaat ttgttcttgc ggggtatttt tcccattgaa
     3601 gatgaaaccg cctgatacaa aaatatccgc ccctgcttta gaacaaactt taatcgtttg
     3661 attatcaatc ttgccatcta cttcaatatc atacgtataa tcattttgtt tgcgtattac
     3721 atctaactgt tcgattttaa ctactgtctc ttgaataaaa tgcttattct ctgttcctgg
     3781 attgattgtc attactaata cttgatcgac taaagataat actggttcaa taaaactgac
     3841 tggtgttgca ggattaaaag taatccctac ttttgctcct gcttccttga ctaactgaat
     3901 gaccttcatt gcgtgtttgg tagcttcaat gtggaaggta acaatatctg ctcctgcttc
     3961 tacaaatctt gtgacaattt tttctggtgt atcaatcatc aaatgaacat caaacacaac
     4021 gtctgttagt ggacgaatat ctttaataac acttggtcca aaagccatat ctggtacaaa
     4081 acagccatcc attacatcaa tatgcaaata atcaacattc ttttctttta agatttcaaa
     4141 taactcttta acttctgcga taggtgcatt taacaatgat ggagcaattg tcattctcta
     4201 ttcccccgtt tattctccca taatcatgag attgattgtt ctagttcgct ctcttagttg
     4261 atcccaatcg acaaaataga cacggcccat ttctcctaat aaaagtttac cttcttgaac
     4321 aattaaagtc attgagttgg ttccataaat agatgaacgt aaatgtgcat cggtatttag
     4381 catgacccat ttttctgcag ggtaattggg atcagataat cttgttccaa aatcaatgtg
     4441 ttttggcccc ggactgttgt attgattttc actcgtcatt tttggaataa tcgtttccat
     4501 tacattgttg atgtctacat gtaaataatc atcaccaaaa aagtttgtgt catgcatcgt
     4561 ttcttcaaaa aagagtgagc aagttgtatg cgggctcgaa attactaaca ttccattctc
     4621 aatacctgct gtatcaactg tatctaaaag ttcttttcga atatcatgat acgaaggacg
     4681 ccctgaaact gtttcaatag tcaaagtgtc atgatgaaaa cgcatattaa acacctaatc
     4741 tttcttttac atctgcataa atggcatctg taccaatacc tgttaataag cccaagccat
     4801 tcgttacttt agctttaatt acatcaggga caattgttgt acttactaca gcatcatatt
     4861 catcaatttt atttagctca tcagcaattt tagattgata aattttcact tgatctgtta
     4921 atccttcttc ttccaaccat tttgataatt ttgctactgc aattgtagaa gtagcatgac
     4981 ctgctccaca aataactaat aattttttca tattgatctc ttctttctta ttttaatttt
     5041 atgattaagc tttcttcact ttgttcatgt agatagaagc acctactaaa acaactccta
     5101 atccacctat tccaattaca ccaaagtaag atgctaataa aacaaacaac cctactggcc
     5161 ataatccaca taacaacata gttactaatc ctgctgtccc cacatcgaaa cttgctaatg
     5221 taaacacatc agtgataatt ggtgccaacc atgttgccat atataatgtt gtaacaacat
     5281 acaacgaaca tcctactaat gatctcacaa tatttccttt aaaataagga atcattaaac
     5341 agatggcgaa agcaaagaat gctaaatctc caaaaggtaa tactttattt cctggaagaa
     5401 ttagtgctag caataatgag attggtacaa gcaatagaga agttgacata atagttggat
     5461 gacccacagt taacgctgca tccattccaa tgtttacttg gcgaccattt aactttttat
     5521 tcgtaaattc tttcgccgct tctgcaattg gcattaaacc ttccataaac attgctacca
     5581 ttttaggcat aatcttcatc actgccgcag tcataattcc taagtttcca attcctgcta
     5641 cgtcatagcc tgccataata cccactacaa taccgataat taggccaata acaactgtgt
     5701 cacctaaaac accgaaacgt tttgttaatg tttctgcatc tgcatctatt ttattaaatc
     5761 caggaatacg atcaaaaacc caatttactg gtttggcaaa tagtactcct gataatccca
     5821 tcatatgtgt gatagagatt cctggatagc cgaaaaattc gctaacagtt ttttgtgtac
     5881 gatctgctaa gaaccattgt aaaattactg cgacaatcgc gccaattaag ccaaaccaaa
     5941 agtttcccgt aacagattga gtaatcatac ctaggaaaat tgcgaaccaa aagttccaaa
     6001 tatcaacatt caatgttttt gtccaaccca aaaccacaaa tatgaagtta agaattaatg
     6061 aaattggaat cattaaaatt cccaatgatg tagagtaagc caaaggtcca ccaacaccta
     6121 cgccaatatc taggatattc aggccaccac cgtattgttc ccccatttgt tgaattgctg
     6181 ttcctaaacc atctgataat aaacttaaaa ctaatcctaa accaatagaa ccgataccaa
     6241 cagtaacgcc ggaaacaaat gctttaccag gttttaatcc aaataatatc ccgattacaa
     6301 aaataatcag tggtaaaaag acgtttgacc ctaatccaat aaaccattca aaggcattta
     6361 atacaatatc catctctcta ttcctcctaa taaactcctg cagaatgtac taactcaata
     6421 aatgcttctt tcgtggtaca tgcaagtaat tcatttactt tttcttcatt ttgaaatagg
     6481 ctaactaagt tttgtagtgt atcaatttgt tcatgaggtt gtgacattgc caacataaag
     6541 actaaacgta caggtatttc atgagattta tctgtcatat ctgaaaatac cagtggttcc
     6601 tttaatgagg caaaacagat ttgagagcta tttacatgtg cggaatctgt atgtgggata
     6661 gcaaacccaa tttgatttac ttctaatcca gttggaaatt cttgttcacg gttggtgata
     6721 ccttctaaga aactatcttt cacgcaacca gcgtttttta attgttcaga cattacttga
     6781 aacaattctt tttgatcttt cacttctaat tgaaataaac ataattcttt atctagatac
     6841 atttttattc tcctttttgt tgaaaaattc ttagtaattt ttcgctatca tgaacttgta
     6901 acaagcgatc gaccatttct ttttgcccaa taaattggta aagttcttca tatactcctc
     6961 taatctcaga tacatcttct tcagctaaac taaccataat gactaatttg acttttcgat
     7021 cgccccaatt aatcgcattt tttaatgcta caataaaaat gttggattca atgaccattg
     7081 atggattcgc atgcggtaaa gcaacaccac tttctaaatt agtattcccc attttctctc
     7141 tagagaagat agaagcacga aactcttcag taactaatga attggcttct agttcggaaa
     7201 tcatcaaatc caaacattct tctttgcttt ccacctgtac tcctgttttt actaaagatg
     7261 attgaacaaa tttagatatg gtgggaactt taaccgcttt aggaatcagt tcattttgaa
     7321 taaatcgcga aggttcttct attaaatatt tagtataagc ttccataata ttcatataat
     7381 ctgttttact taccaatgga gataccctta ctaagggtat accacctgca tcaaaggaaa
     7441 gagaggaaat aatcaaatca acttccccaa tttcagctaa atgaaactta tctaaagttg
     7501 atatttctac attatctttt gctggtaaaa accgtcgaac tttattgtaa attaattgcg
     7561 cagaagaaat accgtattgg caaataatga tgatattatt tgctttggac tgatcttcaa
     7621 tagcaatctg aaaatgaatc aaaattaagg aaacttcatc gtccgttaaa gtcacatcaa
     7681 attcttcttc aaggatggac atggcgtacc atactatgct aaagagttct gaatattgag
     7741 atttgatatt ttctaataac ggattttgaa ttttaatacc tttttttaaa cgaaggacca
     7801 ttgctggcac atgatacaat aaggattgat ataatcgatc attttgagtc aaatcaattt
     7861 tttcaatctc actcattctt tcaatcaatt tatgaacgct catagcgtac tcttcattgc
     7921 tggtttttaa attatttgtc acacgatgtg caaatagttg acgacataga tattctttat
     7981 ctgcgtcata aaatggaaga ttcaattttg ccttcaattc ttcaattaaa ccatttgcca
     8041 caatgtacgt ttccatatag cgaatgctat tgaagaggaa ttcttcttca acagttaaat
     8101 gatgaccttt cagtaaacgg ttacattgga taactaaagt agaaatcaat gaccgaacat
     8161 agtaatcaga aaccttttca gtcagttcac tgtaatcatt gaatagcaaa tcatgaatga
     8221 cactaataac ttttgaatca aacaacatat ttaaaacttg aaaaaatgtt tttgattcca
     8281 tgtcattcgc ataatagaat acaagttgct tgattgctcg ttgaattgct tgttcattac
     8341 cagtaaaact aattccttct gcagatgact gcaacttaac ttctccactg ccaataattt
     8401 tattgataat tctcaggtca ttgtataacg atgttttact aaccataaaa ctttgagaca
     8461 aatgagttaa agaaacaggc ttatcaccaa acaaaacttc tttaatgatc tcgacacgtc
     8521 ttttttctgg agaataaata tctttttcaa gataattttc taccgatgaa atcaaatgtt
     8581 caacgttttt cctatcacct atgagtttga tacccaaaga aggtaatcgc tcaattgtta
     8641 cttggtaatt aactacataa tcttccaaca attcaatatc ttgataaatg gtcttggtcg
     8701 acactgccaa tttatcacta aaaaaagaag cgggtttaaa gtcttcttgt cgacttaata
     8761 attttaataa atttacatgt ctttcaatca aacttgttca cctcttaacg ctagcatttg
     8821 ttacctcttt tcattattta ttctagcact ggaaccccct tccctcaatt acatctatgt
     8881 caataatact tgtggaagtt tgttgtaaag gaaatccaaa taatcttaaa aaatgagaaa
     8941 caaaaaagga gaaatcctta aaagagctaa gatattccaa tagaaaatac tccttattat
     9001 ggaagtaata tgaaatatta caattgaaaa ataaaaacaa tataatggcg tcactaaaat
     9061 tcgtattttt ttattataat ataacttaat tacaaatgct catctatgaa caattgagtc
     9121 ct
//
LOCUS       DAHUVV010000102         9019 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.105, whole genome shotgun sequence.
ACCESSION   DAHUVV010000102 DAHUVV010000000
VERSION     DAHUVV010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.105"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(92..1141)
                     /gene="recA"
                     /locus_tag="NK767_002396"
     CDS             complement(92..1141)
                     /gene="recA"
                     /locus_tag="NK767_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCH6695350.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1250..2491)
                     /locus_tag="NK767_002397"
     CDS             complement(1250..2491)
                     /locus_tag="NK767_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HCH6695351.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2731..3309)
                     /gene="pgsA"
                     /locus_tag="NK767_002398"
     CDS             complement(2731..3309)
                     /gene="pgsA"
                     /locus_tag="NK767_002398"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCH6695352.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3518..4441)
                     /locus_tag="NK767_002399"
     CDS             complement(3518..4441)
                     /locus_tag="NK767_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6695353.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5180..6466)
                     /locus_tag="NK767_002400"
     CDS             complement(5180..6466)
                     /locus_tag="NK767_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCH6695354.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6459..7727)
                     /locus_tag="NK767_002401"
     CDS             complement(6459..7727)
                     /locus_tag="NK767_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCH6695355.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            7979..8440
                     /gene="mscL"
                     /locus_tag="NK767_002402"
     CDS             7979..8440
                     /gene="mscL"
                     /locus_tag="NK767_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCH6695356.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8499..8945)
                     /locus_tag="NK767_002403"
     CDS             complement(8499..8945)
                     /locus_tag="NK767_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6695357.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         2486 a         1956 c         1604 g         2973 t
ORIGIN      
        1 aataactcac gatttcttta ttcaataaaa aaacggcagg tcaaggagag tacctactct
       61 tcagaccagc cgtttttgtt tatcataatt tttattcttc tactaaaggc aattcttctt
      121 gtcctttttc atcttctggg acagcgactt cttcaccaat accataagct gctctaacta
      181 gagcagaaac ttctgccatc atttcaggat gattagccat atagatttta gcgttttccc
      241 gtccttgacc aatccgatct tctttataag aataccaagc accgctttta tcaacaatgt
      301 ctttttctac tgccatgtct agaagttctc cttcttgtga gatccctaaa ccatacatga
      361 catctacttc agcgattttg aaaggaggtg ctactttgtt ttttacgact ttgatctttg
      421 tacggttacc aacaatatct gtaccttgtt tcaattgttc tgcacgacgt acttccaaac
      481 ggatcgttgc gtagaatttc aatgcgcgtc ctccaggagt gatttctgga ttcccaaaca
      541 tcacgcctac tttttcacgg atttggttga tgaaaatagc gattgttttt gttttgttga
      601 tcgaaccaga gagtttacgc aatgcttgag acatcaaacg tgcttgtaac ccgacatgtg
      661 agtcacccat ttcgccgtcg atttctgctc gtggaactaa agcagcaact gaatcgacta
      721 ctacgatatc tacagcccca cttgatacta aagcatcagc gatctctaga ccttgttctc
      781 ctgtgtcagg ctgtgaaaga agtagttcat cgatattcac acctaatttt tgtgcatatt
      841 gcggatctaa cgcatgctca gcatcaatga aagcggccgt tccgccattt ttttgtactt
      901 ctgcaatagc gtgtagtgca actgttgttt tacctgaact ttcaggtcca tatacttcga
      961 tgatacgtcc acgggggtaa ccgccgacac ctaaagcgac atctaacgca agagagccac
     1021 ttggaattgt agagatttgt tggtcgattt tttcgcccaa tttcataata gagcctttac
     1081 catagctctt ttcaattttt ttcagtgcag catctaaggc tgctttacga tcatctgcca
     1141 atacgataat cctccttata tttacaaatc agagcaacat ttcattatcc ttatcatatc
     1201 ttttttagtg aaaaaaagca agaaaaaacc gaacaaatat tcgcatttat cattttctta
     1261 aaagctgttt tctcaaaaga tcaaatcctt gcataaccgc attatatctg atagcagcac
     1321 gatctctggc aaagtgatat ttttccgaaa ccacgccgtc tttagatgaa atagcgatcc
     1381 atactgttcc tgcagattga ccttctaacg tgtccggtcc agccactccg gtgaaagcta
     1441 cactgtaatc tgttcttgct atctcacgtg cttgaatggc catctgctcc acacattctt
     1501 tgctgactgt tccatattct gctaatagct ccggatcaat ctccagaaaa ccagcttttg
     1561 tttggagcga gtaggtaacg aatccgcctg gaaatacttc agatacacca gcaatgtcgc
     1621 ctaatgcggc ttggaaggcg ccggctgtca aactttccgc tgctgttaca gtctgcttgt
     1681 tttctttcag tagttccact acgacttttg ctagcgaatt atcatcgccg tatccataaa
     1741 aatattcccc tactcgttct tggatttttt cttctaatgc taaaagagct tgattccctg
     1801 cctgaacatc atttgttttc actgtcagtc gtaatgtcac ttcatttggt ttggcatatg
     1861 gtgcgatcgt tggattgatc tgtgtctcaa tcagatcttt tagctcagtg accagtcgcg
     1921 attcaccaat cccataaaag cgcaaaactt tagaaattag tttttcttca gaaggaaaac
     1981 gttcctctag taaaggacgt acttgttcca caaacattgg tttcaactca ttaggcggac
     2041 cgggaagaag gatatacgca tgtgtatctg tttgataaaa agtccctaaa gcaagccctg
     2101 tacgatttgg tagaggaacc ccgccttcga taatctggga ctgttgcaga ttgtttttgg
     2161 tcatttttcg atgggttgtt tcaaaatatg ccaataattt tttgtatcct tccgtatttt
     2221 gtatgagaga cttaccgagg tgagccgcag ttgcttcttt tgtcaggtca tcttctgtcg
     2281 gtcctaatcc gccacataag agtatgagtt cacttcttgt ttcagctaga gcaatcaact
     2341 cttctaaacg ctgctgatta tctcctacaa ctgtttgata ataaacttcg atacctagat
     2401 cagctagctg ttctgacaag aaagtggcat tcgtattcac gacttgtcct agtaaaagtt
     2461 ctgttccgac agctattatt tctgctttca ttacttcccg ctcccttata tagtatacgc
     2521 tgtattttta tattttttta tttaaaaaga gggcaaggag ctgtgacaaa aatcttgtca
     2581 cagctccttg ttccaaataa acagtgttct cctgccaaaa cctcggtcat aagtcacaat
     2641 atacataaaa catgaaaagc tgttttttga aattatccct tgtgactcag agttagacgg
     2701 caggctcaca acctctccac taaacgatac ttacattgat cctttgaaca caccgctgtt
     2761 tttgatgaag taatccacac ctgaatagat agtaaagatc agacaagcat aaagcatgat
     2821 ttgatctact ggaataccga tcaggctgaa tgggatatta ttgataaaca gcaagataat
     2881 agcaaccatt tgtgtagccg tcttgacttt tcccggccaa gctgcagcca tgacttctcc
     2941 atgttcgaca agtagtaaac gcaagcctgt gacggctaat tcacgacaga caatgatcgc
     3001 tactacccaa ccaggggcct ttccttggtc aaccaacatg ataaaagcag tcataactag
     3061 cattttatct gccaacgggt ccgcaaactt gccaaaatta gtgacaaggc ctcgcgcacg
     3121 ggcaattttt ccatctaacc agtctgtgat actagctaaa gcaaaaataa ctgcacctac
     3181 tagctgtgtg acagccagtg tggtgtctcc tacgttaaat tgtccccaat ctaaaggtaa
     3241 acctacaacc aaaatgaaaa tcgggatcat acaaattcta agtacagtta atttatttgg
     3301 taaattcaca ctatcgactc ctttcttccc tattctacgt gactatcagc gaagtttcca
     3361 ccgatattat ttacttttcc aaatgaattt attcaaagga gtatttttat tgaaattgat
     3421 ctttttgaat taacgaagaa ggaccgcgac acagatttgt cacgatcctt cttttttata
     3481 cctacaagta aacagatgcg taaactaatt tatacgatca ttctgcgtat tgaagagtaa
     3541 ggttcacatt ttttggactc tgattgtttt cccctggatt aactggtacg gattctccat
     3601 tcgctttgat cgttgcattg cttgctgcac caatcgtgat ggtcgcattt gttgctgttt
     3661 ccggtaacac ggtactctgt gtttcatttg ctgccagtgt cccttgataa acataggcat
     3721 tgtctactag cacacctaac catacacgat tgacagcagt aaattcaagg gttacaggct
     3781 tggtagcgtc tgtgatattt actgcgatgg cactgccaga atcttctcct gtagctattg
     3841 ccattttctt ttcttcactt gattttgttg ttgattctac tgtagaagac tctttggtct
     3901 gcgtgctttg ctctgaggaa gaggactcct cgactgatcc ttctacttta agcgaagaag
     3961 ctgtgtctcc gattattgga tcagcttgac gatcttgcca agtcatatag cccacgacaa
     4021 cgacaatcgc caaagcaacc aatcctaata aaatgacagg aagacttgtc caaaagcgac
     4081 ttggatgcga ttcttcctga tgcattgctt ttctagaagt acttactgtt tcaggctgcg
     4141 gacgttcagg gaccgatcct gtcaatttgc ttttcccatc aaaaacatcg actaaatggt
     4201 ctccatcttc tcctacagct tcagcgtatt gccgaataaa tgcccgcacg taaaatttac
     4261 caggcaattg gtcaaaatca tcattgtcga ttgcttccaa ataacgtttt tgaatttttg
     4321 taatctgctg taattcatct aaagatattt ttttattcag gcgtgcttgc cgtaattttt
     4381 ctccgatggt ttcaaaagcc actcgttcat cttctccagt ctgtttgatt ctccgaagca
     4441 tctgctgttg caacgaaaga cgttatgttt gggtgctgtg acaaaagtca tgtcacagct
     4501 ccttttttcc gaataaacat tgttctcctg ccaactcctc ggtcacaagt cacaatatcc
     4561 aataaatata agaagctgtt ttttgaactt gttccttgtg actcggagtt aaacggcagg
     4621 ctcacgacct ctttcttaaa cggtgttcaa tcagctgttc ctcggtatta gctcaaaaac
     4681 cggcaaaata taaggagata tttttccgta tgtttctcta atactcaggt caaaacgctg
     4741 atttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagtccaa
     4801 ccaagttaaa aacattaaga agctgttttt acttggctgt tcttattact tgatgcaggg
     4861 cagctttttc acaacctctt ggtacacggt gttccagaag tagcttcttc ggcaataagc
     4921 cgaaatatca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct
     4981 gaccacttct gtcacaacct ctttgattac ttcgtttgat tttattttaa tataagttga
     5041 ttaaaagaga gcatgatttg ttttattttc tcactaattt ttccaacaca actaagaata
     5101 ccataaaagc tgattttgga ggaggttcct actacaagtc taaaacgaaa tttaagactt
     5161 ttcctcataa aatactggtt tattttggtc tcatgtaaaa ctcgctgaag gcctcttcac
     5221 ttataaatgc ttccccagct gccagtacgt ctttcatttg gatcgaatcg atgatttcag
     5281 gaagatcaaa taaagttcga tcgccaaata aactttgcgt aaattgattg gcaatatatt
     5341 cgatcgaatt taatgattgg aaatattgtc caagcatttt cttcttcagc aaatcgagat
     5401 ttgtttctga cacttccgga tcatcagcga atccaagaat gatttctttg actttttctg
     5461 ctgctttctc tggttcgtcc gtatctccgc tgaagtccgc aaagtgaaat tcacgatcca
     5521 agctgaattc aaaaccaaag ctgtcatcga taattccttg attgtacatc gctagatagt
     5581 ttcgagaagt attgcctaaa atcatttgga acaacagatt gattgctgtt ttatagcgta
     5641 acagttctgt tccttcttgc ggcaatgtgt ctaatccttt aataccaagt acaaatttat
     5701 ctcgtgtaat ggctgcttcc aatgcacttt gtttgatgat ttcttttgtg ttttcaggga
     5761 aataacgtac aatttcttgc tttggaggga aattttttgc ttcttggttt tcccgaataa
     5821 gtttcatcaa tttctcaggc tccatcttac caactacaaa taaaaccata ttgcttggct
     5881 gataaaacgt ccgatagcac gtatacaagt cttgagctgt gattttgtcg atactttcaa
     5941 ccgtgcccgc aatatcgata tgcaatgggt gtgtaggata caagttattt agtatgccaa
     6001 aaaacatccg ccagttaggg tcgtcttcat acatttggat ttcctgaccg atgattcctt
     6061 tttccttgtt tacagtttct tctgtgaagt aaggtgcttg tacaaaatcg atcagagtcg
     6121 tcagattctt ttctacctga tccgtcgtag aaaacagata gctggttttc gtaaaacttg
     6181 tgaaagcatt ggcggaagct ccttgtttcc caaataattg aaagacatca ccatcttctt
     6241 tttcaaacaa cttatgttct aaaaaatggg caattccatc tggaactttc accttttctt
     6301 tttcaccata gggaataaat tcgttatcga tcgaaccata attcgtactg aacaaaccat
     6361 atgtcttgtg atagtcattt ttaggcaaca aatacacggt caaaccgttt ggcagtactt
     6421 catgataaag tgtttcattg atttgctcat attctgtttt attcatttgc atttcctcct
     6481 gctaaaaaga agattgcctg caattcgatt tgctcagcta cttgttgtat ctctttgatc
     6541 gttacgctct ccatacgttt caaccattct tcatcagata attttgtttc cggcagccag
     6601 ctgtccaaaa aagaagcttc gattgccgct tgcggagagt ctagagaaag cagatattgg
     6661 tttctcagca ttgcttttgt ctgagacagt tccaactctg ttatttcacc atttcgcaaa
     6721 ctttctaatt gctcgtgaat cagatgaagt acttgatttc ggttcttttc atcgataccg
     6781 gtttggacac tcatgaatcc tcggaacgta tccacgctac tagaggcata ataagctaga
     6841 ctttctttct ctctgacatt catgaaaagt ttagagtgag gaaaaccgcc aaataaaccg
     6901 ttaaagacca ttagcgcaaa acgttctggt tcatcgtaat acacatttgt ctgataagct
     6961 aggttcagct ttgcttgaac gattggctca cgtacttgtc gttccttcac gatattcact
     7021 tgaggttggg tataaaacag atctggataa actgtttccc gcgactcaaa tggtaattgc
     7081 ccaactgctt cagctgcttt gtccggatca acatcgccga caacaaaaat gtcgatttga
     7141 tcatttgcca tcatttcttg atagtaggac gctagcgaac tagcagtgat cttgtccaaa
     7201 tcttcgacta cgcccaaccc tgggatcttt tgttccgggc tgttttcaaa ataaagttct
     7261 tgcaaggcat aggaagcaaa agtttgttta tcttccttca aactttccaa atatagacgc
     7321 agattgttct tttccaagtc aaacgtcaac tgatcaaact gatgattttt aatatttgga
     7381 taaaataaaa cctcttttaa aaaatctacc gcttgagaaa aaaggtttgg atcattgaca
     7441 tattcaccat tcacaaaact gatccctgcg tttagccaat gtaaatttcc tttttttcta
     7501 actgataatc caaagcttgc gccataaagc tctgcaagtt gcgcacttaa ctttgtctga
     7561 tccgaataat tcaaactatt tgtttccaac atgctgctga gaagtgaacg tttcgtgatg
     7621 acttcttgtt gcagacgcgc agtaaaacgg ataaaaatcc ggatcgtttt gtattgtttt
     7681 gttggtaaga catgaagatt cacgccttct gccaattgat acaccatatt tatatgctcc
     7741 ttttcacagt tgattgcccg cttactcttt taatgggtca acctgccttt tttacttatt
     7801 cctctgcttg aaatttataa gttcaggcaa ttcgccgttt tttctaaatg atgttacact
     7861 ttatagtata gcatagatgt tttttatcaa aagaaacaga cggctaaaag cgtatattgt
     7921 ttttttattt tttatttttc gatatactaa agacaaataa aagaagggag ggtcattcat
     7981 gatcaaagaa ttcaaagagt tcatcatgcg tggagatgtc ttggatcttg ctgtcggcgt
     8041 tgttattggt agtgcgttta caagcatcgt aaaccaagtg gtgcaaggct tgatcacacc
     8101 actaataggt tggtttgtcg caatgatcac aggtacttct gatttagaag gagcactttc
     8161 aattcttgac tggtcgccga caaaaggcgt cacatttgcg tttggtaacg ttattagtgc
     8221 tatcattaca tttctgatca ctggttttgt cttattctta gttgtcaaag cggcaaaccg
     8281 tgcgaagaat ttacgctcca aagaagagca agtggaagtg gaagaaactc ctactgcaga
     8341 agattatctt agtgacatac gcgatcttct tgccaaacaa gtggcagaag acaatacaca
     8401 tccagtagag caaacaaatc tttccgatac ttctaaataa taaagaatct aaaaaaaaga
     8461 aagccgattt tcagtgtcgg ctttcttttt ttagattctt aacttgctac ttgttccttt
     8521 ttcatgtcga tatgtggaat atcatcttcc aaatacacat ctgagattgc cttgaatcca
     8581 aacgactcat aaaaagcttg taggtattct tgtgcgccga tcacgattgg tttttctgga
     8641 aaatttttct ggatcatgtt aatcgtttct tgaacaatct ttcttcctaa accatcttta
     8701 cggttgtcct gatgaacaag gacacgtcca aaatggatac tctccgcttc ttcatatact
     8761 cgcgtgtagg caaggatctt cccatcagta tcttgaaaaa acgtatgcca agcttctcca
     8821 tcaatgtcat caacttcttg ataaggacac tgttgttcca caacaaaaac tgcgattcgt
     8881 tctttgacga tcgcgtaaaa ctctgctgtt gacaattctt caaatttctt tacctgatat
     8941 tccactaagc aaacgccccc tgctttttta ttcttagcat accgattcgc acacaaaaaa
     9001 gcaaacgaac cttacgaat
//
LOCUS       DAHUVV010000103         8986 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.106, whole genome shotgun sequence.
ACCESSION   DAHUVV010000103 DAHUVV010000000
VERSION     DAHUVV010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.106"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="NK767_002404"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="NK767_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCH6695358.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="NK767_002405"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="NK767_002405"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCH6695359.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="NK767_002406"
     CDS             complement(2169..2906)
                     /locus_tag="NK767_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCH6695360.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="NK767_002407"
     CDS             complement(2997..3524)
                     /locus_tag="NK767_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695361.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="NK767_002408"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="NK767_002408"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCH6695362.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="NK767_002409"
     CDS             complement(4401..4886)
                     /locus_tag="NK767_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCH6695363.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="NK767_002410"
     CDS             complement(5039..5236)
                     /locus_tag="NK767_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695364.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="NK767_002411"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="NK767_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HCH6695365.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="NK767_002412"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="NK767_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HCH6695366.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
BASE COUNT         2537 a         1990 c         1502 g         2957 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgt
//
LOCUS       DAHUVV010000104         8942 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.107, whole genome shotgun sequence.
ACCESSION   DAHUVV010000104 DAHUVV010000000
VERSION     DAHUVV010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.107"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            51..260
                     /locus_tag="NK767_002413"
     CDS             51..260
                     /locus_tag="NK767_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695367.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="NK767_002414"
                     /pseudo
     CDS             467..667
                     /locus_tag="NK767_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="NK767_002415"
     CDS             complement(1379..1984)
                     /locus_tag="NK767_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCH6695368.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="NK767_002416"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="NK767_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2666..2893
                     /locus_tag="NK767_002417"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="NK767_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="NK767_002418"
     CDS             3019..3990
                     /locus_tag="NK767_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6695369.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="NK767_002419"
     CDS             4130..5443
                     /locus_tag="NK767_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCH6695370.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="NK767_002420"
     CDS             5486..6352
                     /locus_tag="NK767_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6695371.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="NK767_002421"
     CDS             6349..7197
                     /locus_tag="NK767_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6695372.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="NK767_002422"
     CDS             7211..8683
                     /locus_tag="NK767_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCH6695373.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..>8942
                     /locus_tag="NK767_002423"
     CDS             8700..>8942
                     /locus_tag="NK767_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCH6695374.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2830 a         1515 c         1831 g         2766 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aa
//
LOCUS       DAHUVV010000105         8761 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.108, whole genome shotgun sequence.
ACCESSION   DAHUVV010000105 DAHUVV010000000
VERSION     DAHUVV010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.108"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(942..1355)
                     /locus_tag="NK767_002424"
     CDS             complement(942..1355)
                     /locus_tag="NK767_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCH6695375.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="NK767_002425"
     CDS             complement(1811..2296)
                     /locus_tag="NK767_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695376.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="NK767_002426"
     CDS             complement(2435..2650)
                     /locus_tag="NK767_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HCH6695377.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="NK767_002427"
     CDS             2874..3674
                     /locus_tag="NK767_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCH6695378.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="NK767_002428"
     CDS             3693..5561
                     /locus_tag="NK767_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCH6695379.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="NK767_002429"
     CDS             5554..6045
                     /locus_tag="NK767_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCH6695380.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /gene="srlA"
                     /locus_tag="NK767_002430"
     CDS             6033..6587
                     /gene="srlA"
                     /locus_tag="NK767_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCH6695381.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..7600
                     /locus_tag="NK767_002431"
     CDS             6605..7600
                     /locus_tag="NK767_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCH6695382.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(7742..>8761)
                     /locus_tag="NK767_002432"
     CDS             complement(7742..>8761)
                     /locus_tag="NK767_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCH6695383.1"
                     /translation="HEDEKLKKLNTLVTIENPFRVKQFSRMFDENKTDRNDALRIADF
                     LRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHFLENLTYKCNTLAREMRDE
                     STSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITM
                     SYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGK
                     VYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLV
                     EAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPR
                     SVIEK"
BASE COUNT         3024 a         1414 c         1670 g         2653 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctga gtttagtcta atggtgataa
     7621 gtcaagatga aatttaagat tttctttatt gagtaatatg ttttgaagcg cacgaaacta
     7681 gacccgatca aaatcaaatt tttttcgggt tgattcaagg atttgcgtca ataacggatt
     7741 gttatttctc tatcacactc cttggtggcg tatagagttg gtgtttacgt agtagcgtat
     7801 ccaccagacg cgtaaatttt cttgcggtta ggacgagtgc acgtttgtgt tgatgctttg
     7861 gaacctcctt atatttactt tgataaaaag ctttgtattc aggaagataa ttttttacag
     7921 agttggcagc ttcaactaag tagtatctaa aatatcgatt tccttgtttt gtcagaggtg
     7981 tattttgcga ctgatagttt ccagattggt tcactttcca acttaatcca gcgtattttg
     8041 ccaattttgt ctgatcttca aatctttcga tttgaccaat ttcagcgatt aaaccggcag
     8101 catagacttt accaacgcct ggaatactgg ttaagcattg atattctgga atgacaacaa
     8161 ttacttgttc gatggcttta tctaattctt tgatattttt ttcaagtgct cgtatttctc
     8221 gtacgagaac acttaaaaca acattaattg attcttgtgc aagagcacct aggcggtaac
     8281 tcatggtaat tgcacgttga attgctttag cgattttttc tggttgcttg aaacggcctt
     8341 ttcctttttc ttgaagtaaa tcacagaaag cttctaaagg aagctcagca agttcatcca
     8401 atgtgaaatc ttccgtcatg agtgaaatga gggttgcact aaaaagactc gtcgattcgt
     8461 ctcgcatttc tcgagcaagc gtattacatt tgtacgttag attttcaaga aaatgttgct
     8521 ttgtacgaat caattgctta ataagttgat agcgagtacg tgttaaacgc tgtaaggcca
     8581 tgtatttctc ttctttgata ggggaagttg tgaatcgttg aatacgcaag aaatcagcga
     8641 ttcttaatgc atcgtttcga tcagttttat tctcatcaaa catgcgacta aattgtttca
     8701 cccgaaaagg attttcaatc gttacaagtg tgttgagctt ctttagtttc tcatcttcat
     8761 g
//
LOCUS       DAHUVV010000106         8549 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.109, whole genome shotgun sequence.
ACCESSION   DAHUVV010000106 DAHUVV010000000
VERSION     DAHUVV010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.109"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(238..915)
                     /gene="nth"
                     /locus_tag="NK767_002433"
     CDS             complement(238..915)
                     /gene="nth"
                     /locus_tag="NK767_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCH6695384.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(922..1620)
                     /locus_tag="NK767_002434"
     CDS             complement(922..1620)
                     /locus_tag="NK767_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCH6695385.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1888..3072
                     /locus_tag="NK767_002435"
     CDS             1888..3072
                     /locus_tag="NK767_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HCH6695386.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3078..3521)
                     /locus_tag="NK767_002436"
     CDS             complement(3078..3521)
                     /locus_tag="NK767_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCH6695387.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3747..4034)
                     /locus_tag="NK767_002437"
     CDS             complement(3747..4034)
                     /locus_tag="NK767_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695388.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4205..5557)
                     /locus_tag="NK767_002438"
     CDS             complement(4205..5557)
                     /locus_tag="NK767_002438"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HCH6695389.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5830..6276)
                     /locus_tag="NK767_002439"
     CDS             complement(5830..6276)
                     /locus_tag="NK767_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCH6695390.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6512..7438)
                     /gene="cysK"
                     /locus_tag="NK767_002440"
     CDS             complement(6512..7438)
                     /gene="cysK"
                     /locus_tag="NK767_002440"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCH6695391.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7578..8465)
                     /locus_tag="NK767_002441"
     CDS             complement(7578..8465)
                     /locus_tag="NK767_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCH6695392.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2325 a         1754 c         1421 g         3049 t
ORIGIN      
        1 tcctttggaa tactggactt attttatagg tatattggaa ggcattattt tctacatcgg
       61 gtattttccc aataaaaata acaactttta tttttatcgc aataaattgt aataagttac
      121 catcctagct actgagtatg tacatttact ttttcatgca aaaaagataa acgaaacagc
      181 ttttctccat aataacaaga taattcatac atttatttgc aattaaagaa accacgtcta
      241 caggcgtggt ttctttttta gcgccttttc ttttagacgc atccgatttt tcccatcttg
      301 acatattgat agcaaaggac atacctcgca cttgggattt cttgccgtgc aatgatatct
      361 accaaaaaaa atcagtgtat gatgtgtctt tacccataat tcttgtggta ctttccgcat
      421 cagtgtttct tctacttcca tgaccgtagc atctagtttg cagatcctca ggcgttttga
      481 gacacgttcc acgtgggtat ctactgcgat tgctggtatc ccgaatgcat cccccaatac
      541 gacatttgct gtctttctgc ctacaccagg aagagacatc aattcttctc ttgacgttgg
      601 cacttgccca tcgaaacgtt caatcagctg ctgtgcacac gctttgatgt tttttgcttt
      661 atttcgatac aaaccaatcg tttttatttt aagaatgatt tcatcgattg aagcttctgc
      721 taacgcatcg ggtgtcggaa aagaggcaaa caaatctggc gttgctttat tcactgatac
      781 atctgtcgcc tgggcactta agatcactgc aatcaataac tcaaatggat tattgtgctt
      841 gagttctcca tgtgcttcgg gaaacattcc atacatcgtt tctagagctt ccattgtttt
      901 ttgtttgctt aacatattcc gctactcctc caaccaattt ttcagtgaga ctttcggcaa
      961 ttcttcttgt tgagctgact gttctacttc tctttgaaga agctgctgtt ttcttcgttt
     1021 ttggtcttct tccacttgtt gttttgtacg tagatttttt ctttcccacg atagcaaaat
     1081 gcggtcaacg taattaaaac tataagcctg atttaatacg gcttcccgca gtgctagctt
     1141 caaaatttcc ggctgataat ggtcttcttc tagccattgt ccgatccgct ggaactcgat
     1201 cgaagaaagc gggcgtccaa attcttgttc aaataattga tacactgact ggatttcttt
     1261 ttcttgtgtt ttttcttcgt attttttttc ttgtgttttt aaatactcac ctaatagatc
     1321 atagatgggg taaagattat atcgatctgc ttttttaccg ttgacaatcg tcgtttctat
     1381 cttgataaat ccatttgtta cgagacgatc cagtatttga aaaatctgat cttgtttgat
     1441 tcccatatcc atagcaatca tctgcaaatc agggaaagta tctccttcaa gctgtgccat
     1501 atgcaactgt agaacaaata aaaattcttc agattgtaga ccgatcctat gataattttt
     1561 aagtaaaaga ttagatatga ctgtttgtcc agcgcttaag taattttcta aattcaacat
     1621 gatttctcct ccgctcaatc acagtatacg taaaaaaaac atgggaagca agaacaggtt
     1681 tcttttctat ttacttcact gatcggtcag atttacaatg tcgtctactt gatttttgtt
     1741 tgttcatgtt tttgatgcgt atcttcactt ttcggaataa ataaattaag tagatttcgc
     1801 tgttccttag aatgttgaca aggaaacaaa attactatac actatttttg tttccaagtt
     1861 aacattattg cataaaggag tttttacatg tacagtttca caaataaaca ttctaaagtt
     1921 tttggatacg tctctgtctt attcactgga ttaggattta ctatcgtcag tcctgtactt
     1981 ccgtttttaa cgctgcctta ttctcactcc atacatcagc aagctttcta tatcactttg
     2041 ctcatgtctg tttatgctct agctgccttc ttatctgccc ccattctcgg tagcttaagt
     2101 gatcattttg ggcgtcatcc gatccttatt atcagtttat taggatcaag tatcggatat
     2161 ttgctttttg gtttaggaaa ttcgatatgg atgttgttcc ttggtcgtat cattgaagga
     2221 ttgacggcag gagaaatctc caccttatat gcttactttg ccgacatcac ggaacctaat
     2281 gaaagaacca aagtttttgg atggatgggt gctttagtcg gtataggaac aacgctgggt
     2341 cctattatag ggggtctatt agctgagttg gggaattctg ttcctatatt tattggagca
     2401 ctctttactt tcttaaatgc cgtttatggc tacacattta tgctagaaag ccttccaata
     2461 aaaaaacgtt cagtagattt aagtttctcc catgtaagac cttttcacca attacaacaa
     2521 ttattcaaga tttcatccgt tattccttta ttgacagcag gttttgctgt ttggttggca
     2581 gctggctctc ttcaatctat cttttctcaa ttttcgattg acgcctttca gtggaaggct
     2641 gggttggttg gtttaagctt ctctttgatt ggaatcttgg atactgtctc gcaattgttc
     2701 attatgcctc gattgctcaa aaaattttca gaacagcaga tcacaagaat agggatgttc
     2761 agtgaaatcc tcgcctatct attcatcaca ttatctggaa ttcttctact gccgattttg
     2821 tttttatttg ggattatctg ttatggtttc ggtgattcta tttttactcc tgtattcaat
     2881 ggccaactat ccaattctgt ctcagataac cagcaaggtc ttgtaatggg cggtacccaa
     2941 agtatacaat ctctatcgcg cgtcatcggc ccgctaatcg caggacagct ttatgctgca
     3001 gcaccatttc ttccgacggt attcggattt ttgcttatga tttttgccta ttatcactac
     3061 aataaaaaat agtagcttca tttgctactt cgtataaatt ctctgtatcg accgataaaa
     3121 cgaaatactg tgtcaaattc ttcttttgac atattttcaa atacagtagc atctcgctgt
     3181 acccattttt gatggagttg ctcgtgtcta ttccttattt tttgtccgat tggcgtcaat
     3241 tgaaaataga tttctttttt attctcttcg ctttgataag tgtttatgat ccctctagct
     3301 atcagttttt ttgttagctt actgatggct cctctagtca tgtaactagc attcgctaat
     3361 tttgtcacgt taggatcagg gattttcccg atatgttcaa tcacttctag ttcatttaga
     3421 gtaaatcctg caaattcttt ttccatttct ggtttattac gatgaataat ctcattagct
     3481 aaatccagat attcttctat tagcagttgt cttttatcca tttctacacc ttctttttct
     3541 ttcattatat tgtttttttg tttcctattc aacaaagata aaaatatttt gtaggaaaac
     3601 ccaatctgaa accaagaaaa aaaggggttg acaaagcttg tcataacccc tttttctgat
     3661 taaaagatag taaagagaca gtcatcggta attgtgatga ttttttgtca tcacttaagg
     3721 atgagcggcc ttttcaaaaa ctgtctttaa agatcatcat cagtttctag cacatatggc
     3781 tgaccttcat atgtgtactc tgtattcaat gtgccgtcat tttcatctga gtagatgcgt
     3841 ctactgaaag gaaaacgatg agaaacatta tctttggcgt catttgctgg catcgtgtaa
     3901 tattcctctt gcgtttcatt caactgagag agtacttgat ctgccaactc atcaatattg
     3961 atcgttaatt tcttcgaagc acttttataa tcttctaaaa atgcatcttg ttggacaaaa
     4021 tattctttat tcatcttctt tctcaccttt cttttcaaaa tcaaataaag ctgcttgttc
     4081 ttttatgtaa cagctttatc tttgctgctt attgtgacaa aaaattcgaa gaaagacaac
     4141 taattgtact tttcacaaaa aaagacagag ggtcgtgaca aaattgtgtc acaacccttt
     4201 ttctttattg tgcagtttcg atttcgccta gcgctgcttg tgcaagtagg ttcaagtaat
     4261 tccatgggcg gtcaaaatgt ggttggaaga agaaatctga aagtgcgaga tcttctacag
     4321 tcattttatt ttgaacagct aaagacatgg tatttgctga ttgtgtaatg tcgtatttgg
     4381 acatgaactg agcacctacg atacgattcg ttcctttttc atagactagc tccatcatta
     4441 ctttttcagt agttggcatg aactctggac gataattatc ttcaaataca gtcgcttgga
     4501 cttctagatc gttcaaagtt gcgctttctt tggtcacacc tgtcgaaccg atcgtccagc
     4561 caaataggta aagtcctgaa gttccttgag taccacgata cgccatttta tgatctgtca
     4621 agttatttcc cacaagcaaa ccttgacgaa cggcatttgt tgccaaagga atatagtttt
     4681 gcgtgccact tggattgtag ttgactactg cagagtctcc agctgcaaaa atgtctggat
     4741 tacttgtctg catgtattca ttcactttga ttgccccgtt tggcagtgta tctacttttc
     4801 cttcgatcag ttttgtattt ggacggaagc ccacacataa aatgaccata tctgtatcaa
     4861 atgtatctgt tggtgtagct acttgtttca cttttccttc ttcatctgtg ataaattcag
     4921 taacattttc gcctagcgca agagtaacac cgcggtctgt caattctttt tctaagatat
     4981 ctgtgaatgg tttatctaga tatttattca agatgcgatc taatccatca atcaatgtca
     5041 cttgttttcc agattcgaca aaagcttcta ctaattcaat accgatatat ccaccaccaa
     5101 caattactac acgttttgct tctttcgctt gtgcaataat ttcatttgct tgattgtagt
     5161 ttttgcatag gagtacattt ttcgcatcga ttccactaat cggtgggata ataggccatg
     5221 aaccagttgt catcactaat ttatcataag aaaccgtttc tgtttctcct gttttcagat
     5281 cttttgccgt tactgtttta ttttctgtat cgacgtctgt tacatcgtgt tccattttaa
     5341 ctgttgctcc taatgaagat aattcttcag gatttgagta gaacaagcct gctgggtcct
     5401 ttactactcc gccgacataa agtgcgatac cgcaagataa gaaagaaata ttgtcatttc
     5461 tttcatatac tgtcacttct gcttctggat gattttttaa aatacttttt acagctgcag
     5521 tacctgcatg agtacagcca attactacta ctttcattta tggtccctcc gattaacatt
     5581 ttgtccttcg acaaaactac tataacagaa ataaaacgga tacaacagtt taatgcttgt
     5641 gaattcacaa aggtttgttt gtagtttcat tccttttcaa gcataagaac ccaatatcta
     5701 caagattctt attttaataa tattcacttt tatatctaag attgtgaata aaagacaaaa
     5761 aaataaaaaa gctagcgttt tttttacaca gctagctttt tttaggtata acacttgtag
     5821 aaactgtttt tacatttgcg ccttttttgc tgactcttta caatccggac agagtccata
     5881 caattccaac cgatgttcat taatctcaaa atcagtcagt ctgcttgcag ccatttctat
     5941 atcttcaagc cctgggtaat gaaaatcaac aatcttaccg cagttttcac aaattacatg
     6001 ataatgacgc tttgaactga aatcaaatcg gctagaagca tcgccgtact tcatttcttg
     6061 aacaaatcca atcgaagtaa ataagcgaag gttattgtaa acagtcgcaa cactcatatt
     6121 tggaaaccga tgttcaagtg ctttataaat ctcatcagcc gttggatggg aatgatgttc
     6181 aatcaaatat tctaaaaccg cgtaccgttg aggtgtgatt cgaatgttct ctttttttaa
     6241 cttttcgata acctcttcga ctaacttatt ttccatgtaa acaccccacg ttgttattct
     6301 cgtcttatca tactattttt acattttaaa atccacttaa catgaaaaga aaattcaatt
     6361 ttattgtatt ttaatgaaaa aagacgtaaa acagtcgcgt tcactattct acgtctctat
     6421 tcaaataatt tttctcatca taaatttttg tgtgttcttt tcatctttaa aagtttttta
     6481 cataaaccta tctggagaaa aaccaagtgc cttatatctc ttggtaaagg gctgtagata
     6541 aataacgttc accattgtcg ggaaccacag ctaatacctt cttgcctttt cctaattctt
     6601 ttgcgacttt tagcgcggca gcaattgcag cgccagatga gatgcctact aaaatccctt
     6661 cttttcgccc aacttctcta gctgtttcca tcgcttcgtc tccactaata gaaaggactt
     6721 tatcatacac agaagtatcc agtgttttag gaacaaaacc tgtaccgatt ccttggattt
     6781 tgtgaggtcc cgcttctttc ccttctaaaa tagcagattc tgctggttct accccgatta
     6841 attcgatttt cggatacaca cgttttaatt ctcggccagc gccagtgatc gttcctcctg
     6901 taccgatacc tgcaacaaac gcgtccaagc catttactcc aaatgcttga tgtatctcag
     6961 gaccagtcgt cttctcatgc acaagaggat ttgcttcatt ttcaaattgt aatggtaaaa
     7021 agtagccgtt ttcttttgcc aaacgttctg cttctctgat tgatccagaa ataccatccg
     7081 caccaggagt caaaatcaac tctgcgccgt accctttcat caataaacgt ctttcgatac
     7141 tcatcgtctc aggcatcacg atgattactt tatatccttt tgcaactcct accatcgaca
     7201 atccgattcc tgtatttcca gaagtaggtt caatgatagt atcacctggt ttcagtagac
     7261 catcgtgttc tgctttttct atcatactta aagcaatacg atcttttaca cttcctcctg
     7321 gattaaaaaa ctcaagcttt acaaaaacat ctgctgaatc ttctggtacg attttattta
     7381 atttgacgat tggcgttttg ccaatcaatt cggtgactga gttatagata gctgtcatct
     7441 tcaattcctc cttgtctttg ctcttcttac ttatcataat acaatctgtt catttatggc
     7501 gagtgattcg tctttgacta gcaaaaaaaa gacaaagata taccatatat ggtaatttct
     7561 ttgccttttt atctaaatca aacgtttagt tcttgaccaa ctaataaaga ataactggac
     7621 atagacatgc catttttcgc cattagatca tcaacagaca tgccaaattt ttgtgcaata
     7681 tcccatagcg tatcacctga cacgactgta tatgtctgac cattatttgt atttacatct
     7741 gttgttgaag atgatgacgt tgaagtcgtc gtagtagtcg ttgttgttgt acttgtggtt
     7801 gttgtagccg aacctgttga tggcgtatca aaacgggtta aattataagt attaatcaat
     7861 gagttcaatt tggccccata atttggatcg gtagcataac gacctgtcaa gtacgctgtt
     7921 gcatcataaa aacttgttgt attgcttttc cacacgccag cataatgata atcgcccgtg
     7981 gaaaaactgg ttgaacgaag aacattcgca tggtcttgga aggattccca ataagaagaa
     8041 tattttcgga atggctcatt catcgttacc cattgtccgt ttacatattc tttggttgcc
     8101 atataaacga cctgtccgtt atagcttcct ttcacaccga atagattata atttggcgca
     8161 ctagctaatc cggaagtgcc atatgcactt tccaacaacg cttgagcgat catgactgaa
     8221 gcatacaagt cattacttgc agcaacttct gacgcagacc agccgatttg atcgataaac
     8281 gcttgaggat cagcgactgt ttgagatcct tgttcatctg catgaacatg ttgcggtacc
     8341 gctgtaacca tcatcggccc aactaaaaga ctcgttccaa ttaaaggagc aatcgctttt
     8401 ttattgatca ccatttttct tttaggtcta tggtgccggg cactgcgggt cttcagctct
     8461 tgcattcaaa ttcctcctca aaataaaaaa acttaataaa aagttctctt cttaaaacaa
     8521 acttaattaa ttaaattata cttttaatt
//
LOCUS       DAHUVV010000107         8511 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.110, whole genome shotgun sequence.
ACCESSION   DAHUVV010000107 DAHUVV010000000
VERSION     DAHUVV010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.110"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..665
                     /locus_tag="NK767_002442"
     CDS             <1..665
                     /locus_tag="NK767_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCH6695393.1"
                     /translation="EQQLKRKELVYLKKYTDSLEKDQEKFARFRHDYKNLLLSLKEIA
                     NQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKNEYLKSLLIAKLYQANNNKICC
                     KFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISN
                     SCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVIL
                     LF"
     gene            680..1432
                     /locus_tag="NK767_002443"
     CDS             680..1432
                     /locus_tag="NK767_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6695394.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2135..2350)
                     /locus_tag="NK767_002444"
     CDS             complement(2135..2350)
                     /locus_tag="NK767_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HCH6695395.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(2369..2605)
                     /locus_tag="NK767_002445"
     CDS             complement(2369..2605)
                     /locus_tag="NK767_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HCH6695396.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2863..5016
                     /locus_tag="NK767_002446"
     CDS             2863..5016
                     /locus_tag="NK767_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HCH6695397.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4985..6400
                     /locus_tag="NK767_002447"
     CDS             4985..6400
                     /locus_tag="NK767_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCH6695398.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            6433..7467
                     /gene="sppA"
                     /locus_tag="NK767_002448"
     CDS             6433..7467
                     /gene="sppA"
                     /locus_tag="NK767_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCH6695399.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            7469..7990
                     /locus_tag="NK767_002449"
     CDS             7469..7990
                     /locus_tag="NK767_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCH6695400.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7987..8373
                     /locus_tag="NK767_002450"
     CDS             7987..8373
                     /locus_tag="NK767_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCH6695401.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         3058 a         1216 c         1519 g         2718 t
ORIGIN      
        1 atgaacagca attgaaaaga aaagaattgg tttatctaaa aaaatatact gattcactgg
       61 aaaaagatca agaaaaattt gccagattta gacatgacta taaaaattta ttgctgagtt
      121 taaaagaaat cgctaatcaa aaccatgatt cgatattgga aaaagaaata aaagaattag
      181 agatctattc taattcttat ctaaccaata agtttgaata tcgttattta aaaaatataa
      241 aaaatgaata tttgaaaagt ttgttgatag cgaagctata ccaagcaaat aacaataaga
      301 tttgttgtaa attcgaatgt ccaagtacta ttattgaagt tcctgttcca atatttgact
      361 gtattagggt gcttggaatt attctcgata atgcaattga agcatctata gagagtcaag
      421 atagagaact gtctctagct atttaccgag atgataaaca gatagaaatt tcgatatcga
      481 atagttgtag agagatacta ctctctatag atactctaat gaaaaaagga ataacaacaa
      541 aaaagggaca tcaagggtta ggtttatcta gtatagagga aatcaataaa aagaatccca
      601 atatgtttgt gaactatcaa aacgatccgt atcaatttac tactcaagtt attttgttgt
      661 tttgagggaa ggaggattta tgagttatcc aataatatta tgtgaagatc aaattataca
      721 attaaatcag ttagaacgaa ttattgataa tttcattctt tttcacgata aagtatttaa
      781 gatagtattg aaaacacaaa gtccgttaga agttaaaaaa tacctcaaac aattccgacc
      841 aaagcaagga atatatttct tagatattga tttaaatcat gaagttaacg gtatagaatt
      901 agcagaagta atcagaaaat atgatgttca agcaaaaatc atttttacaa ctactcatga
      961 tgagatgtta cccgtaacaa taaaaagaag agttgaaacg ttaggatttg taacaaaaga
     1021 tcaaacacta gatgagtatc gaaacgagat tgttgagtta ttgttattag cgcaagaaag
     1081 gatagatgca acaaaacagg ttacgaatca agcatttgtt ttttccatag gttctcaaac
     1141 atttactttt gatataaatg acatttattt tgtagaagct tcaaaattac cacatagatt
     1201 atcattgtgt actaaagatg ggcagtacga attttatgga agaattagtg agcttgaaaa
     1261 gaaatatcct atgttaacaa gaattagtcg agcgtgttta gtaaatatct ttaacgttaa
     1321 agaaatagat tttaaaaaac gttcactata ttttgattca gagttggcca gaaactttac
     1381 tttagggaaa gcacaaaaaa ttaaggagaa gttgaaagaa agcactgttt agtggttttt
     1441 tctttaggga gattgcattt ttctaataaa atagattcct tcactagtgc aaatttttct
     1501 gaatcctatt aaagttgtac caatttttat agctgttatt agttacgtgc ataacagcaa
     1561 tattagttac tggaggaata tttctatggt ctggaaccaa aaaagggcca aatgaatagt
     1621 tcttttcttg tttttattaa gtaccataaa ggggaattaa aattaaatgt atggtggaag
     1681 atgcgaaaga tttggcaaat tctactaatt tttagaaagt gtttttcatt ttttagttaa
     1741 aaaatttgtc ctgtcaagtg caatcctatg attgtacttg ataggatttg agataattac
     1801 tttcctgttt ttataaattt aacttgcagg aacaggcttt aatgcacatc cgacaagtac
     1861 attaccaata cctcctgtaa cggccgaaat aggtcctcct gcagcaccaa ctacagcgct
     1921 gcaagtcctt ttgtaaaaat atctctacca ttacatttcc aaagaatttt tatctgactt
     1981 taataaaaga taaaaattta atataattat aaaaactagc acaagaagct ttagtaaaaa
     2041 gttctgtcca ttccaaactt ttgaaaagat aaagaacata cctatgttcg taatcgacac
     2101 aaaccaatct tgaggtttga tattttttaa ttgtttacgc accggtccac atgctgaatc
     2161 cagcacctaa taaagctcca cctacaccac aagcagctat tgcataaggt cctccggctg
     2221 aacaaagttt tactcctgct gttgccccag aaaaagtagt gcctaaaact cccacggcac
     2281 tcccaccttg aatatttttc aactcttctg aagataatat aaatgttgtt tttgtgtttt
     2341 taatatgcat actacttatt ctcctttttt atttttttcg agcatcccag ccagctgcag
     2401 cacctccagc gacagctcca tataaccctc caacaacacc gcaagctggt ccgcaaattg
     2461 ctgttcctaa acctgcacca cctactgccc ctgtggtaag tccacctaca acttgcccca
     2521 attttcccca agcattattg cctccaccaa cagtttgtgc atttccacaa tacttagttc
     2581 ctttacattt tgcatgttta aacacgcttc tttaaataat attttttacg gtgctactgt
     2641 aacatacgta tttgattaac agtaaaaatc ttcctgaaac ttatcaagtt tattcttgaa
     2701 tagtaaaatt tccaaaataa atttgattca ggaatgaaaa agttagtgtt caagaactgt
     2761 tttcaaagtt tttgtggaat tatgagacaa tattttcact gtattaaagc tatattgaga
     2821 atttttctat aaaaattttt tagaaggagt tgtgttagat aagtgataaa atcatttcct
     2881 tttgtgcagc aacaagatga aaaagattgt ggtgttgctt gcctttcgat gattttaaaa
     2941 tattttaaaa ctgatatccc gattcataaa ttacgggacc tatctgggac tgatttagag
     3001 ggtacttcag catttggatt gaaaaagaca tttgaaaaat tgtcttttga ttgtatggct
     3061 attcaagcag atagtgagat ctggaaagaa agagatctta tcttaccatt aattgcacat
     3121 atattggttg ataacagcta tatgcactac gttgtaattt ataaaacgaa gggaaacgta
     3181 ctatggattg cagatcccgc taatggaaaa aatagaaaaa cgattgaagc gtttcaaaaa
     3241 gaatggacag gtattctttt attacctatg cctagagaaa cttatattcc tattaaagaa
     3301 aaagtagctg gattaagttc ttttttgcct ataatttgga aacaaaaagg attagtattc
     3361 cacatcgttt tagcatcgat tttcattacg ttttttggca taggaagctc atattatttt
     3421 caaggtattc ttgatttttt catccctaat caagcacgtt caactttaaa tataatttcc
     3481 attggcttga tcgctgtcta tttatttcgg gtattgtttg aatatagtcg tagttatctt
     3541 cttattattt taggacagcg aatgagtatg gcaattatgc ttcagtattt caaacatgtg
     3601 ttaactttac caatgaattt ttttgctacg agaaaatcag gggaaattat ttctcgtttt
     3661 cttgatgcca gtaagattat tgatgcttta gcaagtgcta cgttatcaat atttttagat
     3721 attggtatgg tgttgcttgt tggggttacg ttagctattc agaatagtac gttgttttta
     3781 atcactctat gctctttgcc tttttatgta gttgcaatat tcgctttcgt gaaaagctat
     3841 gaaaaatcga acagggaaga aatggcagcc ggtgcagctg taaattctag tatcatagaa
     3901 agtcttaaag gaatagaaac aattaaagca tatagcggtg aagagaaagt atataaccgt
     3961 atagaccatg aatttgttaa attaatgaaa aaatcatttc gtacggttac gcttgataat
     4021 attcaacaag gaattaaaca tttgattcaa ttaattagca gtgcattaat tttatggcta
     4081 ggttcttatt ttgttatgga taatagaatt agtctaggtc aacttatcac atacaatgct
     4141 ttactagtat ttttgacaga tcctttacaa aacattatta atttacaagt gaaaatgcaa
     4201 acggctcaag ttgcaaataa tcgtttaaat gaaattttcg cgattgattc agaaaaatcg
     4261 aaccagtctt ttgaatatat gctttctgaa caattatttc aacaagatat cactgtaaaa
     4321 aacgtttcat tctcgtatag catgaaggcc ccaacgttga acgaaatttc atgtacaatt
     4381 ccttcgcaca gtaaaacggc tatagttgga gttagtggtt ctggaaaatc aactcttgca
     4441 aagttatttg ttcgttttta tgaagcatca gaaggatcga tccattatgg aaacattaat
     4501 cacttagaca ttccttttca gatattacgc gatcgagtga catatttgcc tcaagagtca
     4561 tttttcttta gtggaacaat cattgagaat cttatattcg gtttagatag cccaccaagt
     4621 ttcgaacaaa taatagaagt ttgtgagata gtccaactaa atgaatttat taatcaacag
     4681 ccattacgtt acgagacatt gttggaagaa ggtggaaata atctttcagg agggcaacga
     4741 caacgattag ccttagcacg ggctttatta aaaaatccgg acatattaat tttagatgaa
     4801 gcaactagtg gattagatac cttgttagaa catgcaatta tgaattatct cctcaattta
     4861 aaagaaaaaa cgattatttt tattgcgcat catttaccaa tagcaaaagc atgtgatcaa
     4921 attttagttc tccatgaagg gcatttggta gaaaaaggaa cgcacgatga acttcgtttt
     4981 gccgatggta tctatcaaaa attatgggaa atctaggaga gggaaacaat ttatgcagaa
     5041 taataattgg ttcgaacatt ctagtatcta tactaaacaa catcatagct tctaccgttg
     5101 ggtgttatac cctgtaattc tttttttatg tattattgga ttgtttttaa cctttgctaa
     5161 aaaagaggtt gttattcgga caacggctca attgaacccc gaaaaaatag agaagttaca
     5221 agtgccatta gaagctaaaa taatggaaaa cttcctaaaa gaaaatcaat ttgttcataa
     5281 ggacgatgtg ttggttagat tggattgttc tttaatagag aatgaaaagg ctcagataga
     5341 acaagaaaac caacgtatta ctcaacaaat aaagatggct cagctattta ttgaaagtat
     5401 aagtaaagga aaaaatttgt tttcaacgga tgacagtttt ggctacagta atcaattaaa
     5461 gagcatgttg tcagaaaaag aatcactccg ctacgctttg aagcaaagtg aattaaatga
     5521 tcaaaagcaa ttagaagtat acgaaaagac aaaaagacaa ctagaaaaac aaattgagag
     5581 ttcagatagt aaattacaag aatggcaaca agtacaggta gcttggagta ataatcaatc
     5641 attaaaagat ttttcaaaag aaatgatggc aaactatgag aattggcaag aacaactaaa
     5701 taatgtttct gatgatcaaa aaaatcaagt gaaactgaca atttcagcaa gcataaatga
     5761 acaaattgag caactaaaaa aagaagtaga acagtatcag tcagaaaaag ctaaattagt
     5821 taaaccaact acttctgaga atgacagaat tagtcaaacg gaaaaaggaa agcaagagct
     5881 agaacaagca ataacaactg taaaacaaac agttacagag ctaaatgaaa aacaagagaa
     5941 gaaccagtca gttatcaaaa gtttggacga tcaactttca aaaggaatac taaaagctcc
     6001 tgtatctgga acggttcatt taaatgaaga aacaaaggga caagcagaac tagccaaagg
     6061 aacagtgctt gcagaaattt atccgatcca cgaaaaatca aaaatgaaat ttacagcact
     6121 tttacctgcg aatgaaagta cctatattaa agaagggatg aaggttcatt ttaaattgga
     6181 tcaaaaaggt ggggctccga ttacgatcga cggaacttta gatgaaattt cagaaaattc
     6241 tactgccact gagaaaggtg ttttttatgc agtgaaagga acgttaaaaa caactcaatc
     6301 gtttccgttt cgttatggat taactggaga gttgtcttta attgtaggag aaaaaacata
     6361 ttggcaacaa attgtcgatg tgtttctaaa taaaaaataa ttatttttac taagaatata
     6421 ggaggagttt atatgaataa aagaagatgg attgctgtgg gagtagcgtg cggattattg
     6481 ttactatcca ttatcgtcgc gctaattcca ggaaaagata aagaggaagc atcaaataca
     6541 accctaacag gaataaataa gattttttac ggatctaacg aaatcacaga agaaacgtta
     6601 gaagaaggcg catcaaataa aaagattgtc aaactatccg tcaatggagt tattgcagat
     6661 acaggtgaaa gcaatctttt tagtagggaa caatataacc atcagaattt tttaacacag
     6721 ctaaagaaaa ttcaagaaga caaagcggtt aagggagtgt tattagaagt aaattcacct
     6781 gggggaggaa tatatgaaag tgctgagatt gctaaagaaa tggcaaaaat taaaaagcta
     6841 gatattccta tctatactgc atttaaaaat acagcggcaa gtggagggta ctacatttct
     6901 gctggttcag ataaaatatt tgctacggaa gaaacaacaa ctggatcaat tggtgtcatt
     6961 atttctggtt taaattattc tggcttatta gaaaaacttg gcgtaactga tgcgacgtat
     7021 aaaagtggtg cattaaaaga tatgatgtca cctcaacaca aaccttcaga ggaagagaat
     7081 aaagttatac aggaatttgt gatgagtgct tatgatcgtt ttgtcaatgt tgtagcaaaa
     7141 ggaagaaata tggatactaa tgcagtgaaa gaacttgcgg atggaagaat ttatgatggt
     7201 aaccaagcag tagaaaatgg gttggtggat caaattggtt actctgaaga tgcattagat
     7261 tccctaaaaa aagagaagaa attaactgat gcgacggtta tagaatataa aaatgacaca
     7321 actggctttg cttcttcttg gttaggtaat aagattgctg aatggcaagg gctaaaggca
     7381 actacatcag atcgtgtaat ttcagttttt gaaaaacttg gattttctga tactccaaaa
     7441 ccaatgtatt actatggagg gctataaaat gaataagaag agtggcattg aaactttgcc
     7501 taattatgta tttgcgggat tttggttacg attttttgct tttcttattg atttaatttg
     7561 tattagcatc atcacccatg ctactatagg tctcttttac aaaatcatgg gactggaatc
     7621 tgacaaatcc attattactt tttatggtat agcgtcatta gctatttatt taacctattt
     7681 tgtacttttg actaagctga attcaggaca aacaattggg aaaatgattt ttgggataag
     7741 agtagtgagc tttgaggaaa aagagctaag ttggcaaact atctttattc gtgagtgtat
     7801 atgtaggttt atattaaaaa attttgtatt tcaaattggg tatttggtgg ctgctttcac
     7861 gcccaagaaa caacatattg gggattattt ttctaacacg tcagtggttg ttattcgttc
     7921 attagaagct attaactatg gtcaaaaaat ttctaattct aaaacactta atctggagag
     7981 aattaaatga ataagagaaa aacagagttt attttgattt taattagtgg aataacaggg
     8041 attttatgta gtcttttagc attgatgggt ggtatgtttc tatcttctat tgattcaact
     8101 actttaaata ctacagaact aaccagtaag gatatagata tgttagtcaa tctaagcggg
     8161 aatgttgttt tatttggagg aattacccta gttttttcga taatgatttt cataataggt
     8221 tttttagtaa gaaaaaatca aaaaacagtg gtctatggta ttagtttact cattttatct
     8281 attattccat tttttctttt aacattattg tgggtaattc ctggagtttt aggtattatt
     8341 gcgggattga tgcttctaat aagaaaatct taattaggca atgtgtagac aacgtgaaac
     8401 gattaatcaa ttaaaagcta aatacgtttt gagaaatgat aacttgtagt cgatggaaga
     8461 catgtagata aaaaaactac gtgtctttta tttattctaa ttttttcgaa a
//
LOCUS       DAHUVV010000108         8494 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.111, whole genome shotgun sequence.
ACCESSION   DAHUVV010000108 DAHUVV010000000
VERSION     DAHUVV010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.111"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..173)
                     /locus_tag="NK767_002451"
     CDS             complement(<1..173)
                     /locus_tag="NK767_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6695402.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="NK767_002452"
     CDS             complement(593..1714)
                     /locus_tag="NK767_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCH6695403.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="NK767_002453"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="NK767_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCH6695404.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="NK767_002454"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="NK767_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HCH6695405.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="NK767_002455"
     CDS             complement(4304..5215)
                     /locus_tag="NK767_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCH6695406.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="NK767_002456"
     CDS             complement(5232..6239)
                     /locus_tag="NK767_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCH6695407.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="NK767_002457"
     CDS             complement(6236..8365)
                     /locus_tag="NK767_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCH6695408.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="NK767_002458"
     CDS             complement(8370..>8494)
                     /locus_tag="NK767_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCH6695409.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAHUVV010000109         8374 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.112, whole genome shotgun sequence.
ACCESSION   DAHUVV010000109 DAHUVV010000000
VERSION     DAHUVV010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.112"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..570)
                     /locus_tag="NK767_002459"
     CDS             complement(<1..570)
                     /locus_tag="NK767_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCH6695410.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKILRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKE"
     gene            complement(560..1642)
                     /locus_tag="NK767_002460"
     CDS             complement(560..1642)
                     /locus_tag="NK767_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013554917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCH6695411.1"
                     /translation="MSEEGYEQEYVKEAFDTNWVAPLGANVNGFEKELAEYVGASHAA
                     ALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPVFVDSDYETWNMK
                     PEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIEDAAESLGTTYKGK
                     YTGTFGDYGIYSFNGNKIITTSGGGMLVSNEKEKVEKVRFWATQSREKARHYQHNEIG
                     YNYRMSNIVAGIGRGQLKVLNERIEKKRYIHNFYKKELSKLSNIQFMPENNWNEPNYW
                     LSVITIKGINTNHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGDDVAETLFDLGVC
                     LPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            complement(1680..2312)
                     /locus_tag="NK767_002461"
     CDS             complement(1680..2312)
                     /locus_tag="NK767_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCH6695412.1"
                     /translation="MKAIIVGAGGFGKEIAFLLQSVSRYELVGFVDDSLKMQNQELLE
                     KPILGTIDLLIELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVAPSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSVFPSVNISGHVILGD
                     KNEIGVGTKIIQNLSIGNTNIIGAGSVVIKNINNNTKNVGVPTKVIERWD"
     gene            complement(2323..4140)
                     /locus_tag="NK767_002462"
     CDS             complement(2323..4140)
                     /locus_tag="NK767_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCH6695413.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAVFGSLSASAVSSIIILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYVETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAARVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(4153..4917)
                     /locus_tag="NK767_002463"
     CDS             complement(4153..4917)
                     /locus_tag="NK767_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HCH6695414.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            complement(4945..5643)
                     /locus_tag="NK767_002464"
     CDS             complement(4945..5643)
                     /locus_tag="NK767_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HCH6695415.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQVLAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(5655..6434)
                     /locus_tag="NK767_002465"
     CDS             complement(5655..6434)
                     /locus_tag="NK767_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HCH6695416.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(6450..7394)
                     /locus_tag="NK767_002466"
     CDS             complement(6450..7394)
                     /locus_tag="NK767_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCH6695417.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7440..8219)
                     /locus_tag="NK767_002467"
     CDS             complement(7440..8219)
                     /locus_tag="NK767_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695418.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2265 a         1739 c         1416 g         2954 t
ORIGIN      
        1 ctctttcttg attacttttt tacatgtctg aattataata ataagatcat taaagaaact
       61 aattttatca acataataaa catctaaatc aaacttttct tcccaactaa tcgcatttct
      121 tccattgacc tgagctagtc cagttaatcc tggtcttacg ttgtgtcttt tccgttgctt
      181 ctcactatat agtggcaaat actctactaa taatggacga ggtcctacga tcgacatatc
      241 acccttcaaa atattccaaa gttctggtag ctcatctaga cttgttgctc taagtatttt
      301 cccaaattta gttagtcgaa tttcatcagg taaatattct ccattgtggt ctctttcgtc
      361 agtcattgta cgaaatttat acatggaaaa aattttttca ctttttcctg gtcgttcttg
      421 tttgaagata attggagaac caagtttaat tcttactaaa aaacctacaa ttaagattat
      481 cggcgataaa acgatgagag caatgcctga caataggata tctaaaattc ttttcccaaa
      541 aagctgatag aaagtttttt tacgcatcat tgatcactct tttaatcgta gatactacat
      601 attttaaatc ttcatctgtc atctttgtat ctgatggtaa acatactcct aagtcaaata
      661 aagtttctgc tacatcatcg ccaatataat cataattttt gaaaacaggt tgtaaatgca
      721 taggtttcca tactgggcgg gcttctatgt tatttttatc taactcatca ataagatgat
      781 tcgtatttat cccctttatt gttatgacgc ttaaccaata gttcggttca ttccaattat
      841 tttctggcat gaattgtata tttgacaatt tagacaattc ttttttataa aaattatgaa
      901 tatatcgttt tttctcaatt ctttcattta aaactttcaa ttgtcctcga ccgatacctg
      961 caacgatatt gctcattcga taattatagc caatttcatt atgttgataa tggcgtgcct
     1021 tctcacgaga ttgtgtagcc caaaatctca ctttttccac cttttctttt tcattagaca
     1081 caagcatccc gccacctgaa gttgtgatta tcttatttcc attaaaagaa taaattccat
     1141 agtcgccgaa tgtaccagta tattttcctt tatatgttgt tcctaatgat tctgcagcat
     1201 cttcaattag agtaacatca tattctttgc atattttagt aatttcgtct aagttagctg
     1261 ataaaccgta caaatgtact actaatacag cctttgtgtt aggatatttt tctagcgctg
     1321 ttcgcaaatg ttcaggtttc atgttccaag tttcgtaatc actatcaaca aatacaggta
     1381 tagctccctg atagagaatt ggattagcag tagctgagaa cgtcaaggac tgacaaatca
     1441 ctatgtcatc tttaccgacc ccagccgctt tcaaagctaa atgtatagct gctgttccag
     1501 aagataatgc ggcagcatga ctagctccca cgtattctgc taattctttc tcaaatccat
     1561 tcacattcgc acctaaagga gccacccaat ttgtatcaaa tgcttctttt acatactctt
     1621 gttcatatcc ttcctcactc atatgaggag atgctaataa tattcgttta ttttccaact
     1681 taatcccatc tttctattac tttggtaggt acgccaacat ttttagtatt gttattaata
     1741 ttttttatta ctacacttcc tgcacctata atattcgtat ttccaatact tagattttgt
     1801 attattttag tacctactcc aatttcattt ttatctccta atataacatg tcctgaaata
     1861 tttacacttg gaaaaactga attataatta ccgatttgag tatcatgtcc gatagtagag
     1921 ccgatattga tcatattgaa atttcctaaa actacgtcag cagtgtaagt cgtatagggc
     1981 atcaaaatat tgccaattcc taactgaaca tttatgccca ctagtgcact tggtgcaact
     2041 aaattaggga aaatcaattt attattttta tttatcttct gatatatttt ctctttgata
     2101 tctggcgaag caattcccag aaaaatagca gtttcttctt ctaattctat taacaagtct
     2161 attgttccta atataggttt ttccaataat tcttgatttt gcatttttaa cgaatcatcg
     2221 acaaaaccaa ccaattcata tctagagaca ctttgtaata gaaaagcaat ctctttgcca
     2281 aatccaccag ctcctacaat aattgctttc actttactcc ccctacttat ttgaagcatt
     2341 tgcaaaagtg acaatgtctt tagccagttg ttcatcgtct tgcggcaggc tcttgacaaa
     2401 atccatcact ttttgtattg agtaaccttt gatgtttcct acaaagattt tctcataaac
     2461 tgcttcatca tttctttcct tatccaacaa tagttcttca tatagttttt cccctggacg
     2521 gatacctgtt tcaatgattt cgatttcgtc ttcgctatac ccgctcaatc ggatcatgtt
     2581 tttggctagg tctacaatct tcactggttc actcatatct agaataaaaa tttcgccacc
     2641 ttttgctaat gcgccagatt gaataactaa acgactcgct tcaggaatcg tcatgaagta
     2701 tcgggtcata cgaaagtctg ttacagtgat tggaccacct tgagcaattt gttctctgaa
     2761 tactggaatc acactaccgc gagaaccaag gacattccca aatctgacag ctgagaattt
     2821 cgtacagcct tcttcattca atccagtcac gatcatttca gcgattcgtt ttgttgaacc
     2881 catgacgttt ggaggattgt tagctttatc tgtcgatacc atgacgaagt ttttcactct
     2941 tgctgctttg gctgcttctg ctacattttt tgtaccgaaa atattgtttt tgactgcttc
     3001 ttttggatta tattccatca gtggtacatg tttatgagca gctgcatggt agacgatatc
     3061 tggatgatat tgttccatga tctcattgat tttctcgcga tcttggatat ctgcgatgat
     3121 cggtacgatt tctgtaggac aattttgatg atgcgtgcgt aactcacggt caatcaagta
     3181 gatcgagttc tccccatgtc ctaaaagcaa tagctttgca ggattgaatt gaatgatttg
     3241 ccgacagatt tctgaaccga tcgatcctcc tgcgccggtg acaagaatga ctttgtctgt
     3301 gatctgatct ttgatggatt caatatccag ctttacctca tctcttccca gtaaatccac
     3361 tacgtcaatt gtttttaatt ttgatacgct aatttttcct gaagcaagtt cttctataga
     3421 aggcatcgtg ttgactttca cacgagcgga ttcgactaac tcgaagattc ttcgtaattt
     3481 ttttcttgat aaggaaggga tagcgatcgt taccatttga atgtcatatt tttcaatcaa
     3541 ttccggaata tcttttaatg cacctaatac tttttttcct gaaagatacg tatttctttt
     3601 atttggatca tcatctacaa acccgacaac gtggatatct tgagccgttt tggatccaag
     3661 aaagctgttg tataaaatac gcccgccttc gccagcaccg acaatcaatg tatttttagc
     3721 agaatctgtg gatagatatc tcatattttt tgtttcgaca tagatacgcc agatcaatcg
     3781 gctaccaata atcaataata gcgaaagcag gtaagcaaaa atgactaaac gtaaactgta
     3841 tcgccgattt atgaacagca atatgatgat acttgagaca gcacttgcag atagtgagcc
     3901 aaaaaccgca atgatttctc ttaaatttgt atagcgattg atccgagtga atactttgaa
     3961 taaactacca tagaataaat aaaaaccaat cgacaaaccg atagagatta aaataaggtc
     4021 cattggtata gctacaaacg gcttcatgaa tttagctgct gcaatattcg cagcaatcaa
     4081 caacaagctg tccacaaata ccaatgtggc aatttttctt cttcttgtta atacaaacac
     4141 cctacattcc ccctaaaata aaccaaactt cttctttttt acctcttcat agattggata
     4201 actgatttca tccccgttga tcagatcctt tgcgacttgt ttcatctgta ctactttttc
     4261 cttaccaaag tccttggcaa tctgttcata gcactctttt aagtaaaagg tgcgcttaga
     4321 gaccccatgg gcatcagaag cagccatctg cacaagatta tgtttgacca tcgttttggc
     4381 tgttttctgg atgtctttgc caaatacccc aacataactt ggcgcagtga gttgggccaa
     4441 acatcccata tctaggaacg gaagcaaatg atttgggtct tcgcgaaaat gcgcgttacg
     4501 ttctggatgg acgatcactg gtgtctttcc tagttctaac aactcaaaaa aaagccgttc
     4561 tgcatataac ggcacttcca gtgtcggaaa ttcgatcaga agatatgtat catctaaatc
     4621 tgtaaataaa atttcatctc ggtggatgtc ttcgatgagc gttccggtaa tacgtacttc
     4681 ttgtccttcc aataacgtca aaggcaactg tcttttctct aattcggctt gtaacgaagc
     4741 gactagagaa atgacttgtg atttttcatt gctgtatttt ccattattgt ggtgtggtgt
     4801 gcataaaatg tgcgtgatgc cttgctgaat cgctttttca gccattgcaa tacttgcttc
     4861 taagttttct gccccgtcat cgattcctgg taggatatgg caatgtagat cgatcatttc
     4921 tcttgtctcc tctcttctta ttccttaatt tccgtagtaa taaccagcat ctttgctatg
     4981 ttctgctccg ttgtacacaa cacctaagac tcgtgcttgt accatctcca gcagttcttt
     5041 tgctttagtc aaggattctt tacgtgacat attttcacgt acgaccaata aagtgccatc
     5101 cgtttttgaa gacatgatct gtgcatctgt tacggctact actggtggca tatcgaatat
     5161 caccacgtca aataactgtc ttgcttcggc tagaacctga tccattcttg gcgagcttaa
     5221 caattctgat gggttgggtg gcttaggtcc actaggcaaa attgacaagt tctcgactgg
     5281 cgttcgttgg atcacatctg ctacacttcc tgatgaacta agtgctgtac ttaatccact
     5341 ggcattgttc agctggaatg tcttatagac gacgggttta cgtaaatctg catcgactag
     5401 tagaactcgt tgacctgatt tagcaaaaac gactgcaata tttgctgcag tggtggattt
     5461 cccttctcca ggacctgatg aagtcacgac aatcgttttg atttgctgac cagctgcgga
     5521 ggcaaactgg atattcgtcc ggatcgtacg atattgttcg gcgattggtg aagatggatt
     5581 tgttaatgtg atcaaactga ctgcatgagt ttgtgctgct ttttgtttct gtgttcgtgc
     5641 catttttctt cctcctagac tcttgagcgg cttctgcgtg atggggctgt tttatcgttt
     5701 gtagcttttg gtcgtgcagg agctgttcct tttttacgga taccagcgtt caattctttc
     5761 gctgacattt ctggaactgt tcctaagatt gggaatccta atgtatccgt cacatatttt
     5821 tcatctttca ctgtccgatc aagtaattcg aataagaatg ccaaacctac gcctaccatc
     5881 attcctaaca ctaagccaat cactagattc aatttattat ttggcgaaac aggtgtcgta
     5941 ttggcaactg cacctgaaat aatcgagatt ttatcgacat tcatcacgtc tttggcgttt
     6001 tcttggaaaa cttgtgaagt tgtgttagcg atctgttgag cagtcactgc attcgtgctt
     6061 attgcctgga tcgagaacat ttgtgaattt tgtgactgat tgacggagat cgcatctttg
     6121 atttgtccag cagtcatttt cacattgtct tctgtttcta aacgatcttt gacttcattt
     6181 agtactaaat cactgacgat catatcttta tacgtattga tcatttgcag gttcgtattg
     6241 acatcattga catttgctgt gtctgattgt ggcagtgtca cgatcaattg tgcttgtgaa
     6301 ctgtattttg gtgtcaagat gaaaaacgtc acgattcctg agatggccaa gccagcaaac
     6361 attgagatga tgattgttac caagtgtttg cgtaatgttt caaaaatttc tttcaagctg
     6421 attgtctctt ccattttttc tccactttcc tatttattct ctagattcat ttgatctttt
     6481 aattcttttt ttacacgatc aagttcttga tcactgactt tttgatagga gatattatct
     6541 tgcatgaagc cttctccttg cagctgatct tgcttgatcg taccaaatgc ctcccgataa
     6601 tctaaagcaa tttttttcat ttcatcaaag ctcatgtctg ttttcatatt cgactctaga
     6661 gcattcaaaa cggattgata gttgctgaca ccgtttagac tcaatacttt ttttgctacg
     6721 ccttctacga tctttctttg acgctcttgt ctaccatagt caccgtttgg atcttcataa
     6781 cgcatacgag aataagataa tgcctgatcc ccgttcaaag taattttacc gattgtaaag
     6841 tcatagccat cttgtgagaa agtgaggtca ttattgactt cgataccacc aactgcatca
     6901 acaagttctt ttagcccttt catgttgata gagacatagt gatcgattgg gatgtctaca
     6961 tatttttgta cggtatccat cgccatggca gctccgccga atgcgtaagc gtggttgatc
     7021 ttgtcttttg tgtcacgacc tacgatgtcg acatacgtat ctcgaggaat gctgacgatc
     7081 gttgtctgtt tgtcttcagg actgactgtg gcaaccatca tcgtatctga tcttccttga
     7141 tcgacccgac ctaaatctcc agtatcaatc cccatcaata gaacagagaa agattcttgt
     7201 ttgtccagat caactggttt cgttcgttct ccttgggttc gttccactga ttcgtaagtt
     7261 ttctcaatag aatgtgagag atccagatat aattttgctc ctactcctac aattgctaat
     7321 actaatacga taagaactgc taaaataatc agaaatattt ttttccctct tgattttctt
     7381 cttcgtcttg ccatagtttc ctccgtttgc taaccttgct tgaatcaacg ttttaactgt
     7441 caatagctta tattgctata gcccatttgc tgagcaacag tttgtgcttt tgttgcgtat
     7501 gctgcgcgta ttttttcatt ccggatctgc tctatttgac tgagcaccgt caaatattga
     7561 tcgtaagtag cggtggcagt cacttgacca tccttcaaca tcgtatcgaa taactgatct
     7621 agttgcgtga cttgcgcgat ctgagtatca gcgaatccta gatattgcat aacaaccgtt
     7681 ttccaaggac tatctttaaa aaagctcata ttttcacgta cacgagcgac atctgcactt
     7741 gtcacctttt ctttgatgat gacatcgtct acagcttgct gccagtttga tacattttta
     7801 gtaaataaat cgttgacagc tgtctggatc ttcagttttg attctgcact atctaactgt
     7861 gtcacgattg ctttcttctc atcttggatt gcaacagctt ctttaggcaa ttctgattct
     7921 ttgatgtcgt aatcatctgc tgaaagtttg acagaggata gcttatttga caaacttgtg
     7981 attgtttctt ctgttatttc agggtttaga aaaatttttt cttttgtact atacagcttg
     8041 tctacttctt ttgataaaga agtcaaggtg tctttttgat tggtgatggc agattctgca
     8101 taattgatcc gttgctgatg ttgtcgctga ttattgatat ggtaaccaaa caaacagatc
     8161 gcaatagcac ctgcacctac cgccacagca gtcaatgact tgacgaatgg cttttccata
     8221 gattcactcc taagtattga tgatagataa attaagatat aattcactta cataaaaatg
     8281 acagcttttt attttaaaaa acataattaa actaatatat tcacagaaat atttgtaaac
     8341 aagttttccc tatataatca taccacaaac tgtt
//
LOCUS       DAHUVV010000110         8236 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.113, whole genome shotgun sequence.
ACCESSION   DAHUVV010000110 DAHUVV010000000
VERSION     DAHUVV010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.113"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            13..486
                     /locus_tag="NK767_002468"
     CDS             13..486
                     /locus_tag="NK767_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HCH6695419.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            495..722
                     /locus_tag="NK767_002469"
     CDS             495..722
                     /locus_tag="NK767_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6695420.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1358..1729
                     /locus_tag="NK767_002470"
     CDS             1358..1729
                     /locus_tag="NK767_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695421.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1985..2227)
                     /locus_tag="NK767_002471"
     CDS             complement(1985..2227)
                     /locus_tag="NK767_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCH6695422.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2259..3161)
                     /locus_tag="NK767_002472"
     CDS             complement(2259..3161)
                     /locus_tag="NK767_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCH6695423.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3174..3362)
                     /locus_tag="NK767_002473"
     CDS             complement(3174..3362)
                     /locus_tag="NK767_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCH6695424.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3376..3939)
                     /gene="amaP"
                     /locus_tag="NK767_002474"
     CDS             complement(3376..3939)
                     /gene="amaP"
                     /locus_tag="NK767_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCH6695425.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3977..4870)
                     /locus_tag="NK767_002475"
     CDS             complement(3977..4870)
                     /locus_tag="NK767_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCH6695426.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4948..5871)
                     /locus_tag="NK767_002476"
     CDS             complement(4948..5871)
                     /locus_tag="NK767_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCH6695427.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5920..6270)
                     /locus_tag="NK767_002477"
     CDS             complement(5920..6270)
                     /locus_tag="NK767_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCH6695428.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6303..7205)
                     /locus_tag="NK767_002478"
     CDS             complement(6303..7205)
                     /locus_tag="NK767_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCH6695429.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7198..8055)
                     /locus_tag="NK767_002479"
     CDS             complement(7198..8055)
                     /locus_tag="NK767_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCH6695430.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
BASE COUNT         2257 a         1587 c         1397 g         2995 t
ORIGIN      
        1 agagggataa aaatgaaatt taaaacattg ctattaaaat ttgtcgtttt aggtataagt
       61 gccgtattta ttttgttcgg cgtacttttt tatactcaga tcgctacaag cgaaaaagga
      121 ttcaattttg attggcagac cacattattt gtttctgtca tttactttgc ccttctccta
      181 gggatcgtta ttgcttattt cttattgaaa ctattgaaaa aaatcgaaga taaccaagct
      241 ttttccactc aaagcttaaa catactaaaa aaaatcgaac gaaccatttt gtttattggt
      301 ttcgcctttt ttggcttact tcctaaattt atgaaagtag cggatttaga cgatgctcca
      361 ggtttgatgt tggtaggaat cgctgtcgta ttcattcctt ttgcagtata cacattaaca
      421 agcgtgctgg aaaaactgct tgaacatgcg attttactta agacagatca tgatttgaca
      481 gtgtgaggtg cgacatggca attatcgtta atttagatgt agtattagct caaaaaaaaa
      541 tgagtgtgac tgaattgagt gagaaagtgg gcattacgat ggcaaacatc tctattctaa
      601 aaaatggaaa agcaaaagcc atccgttttt ccactctcga aaaaatctgc caagttttgg
      661 agtgtcaacc aggtgatttg ttgacctata aaaatgactc gaatactata ccagaagaat
      721 aaaataaagt agaacaaaca gtaaataaaa aataatctga atgatagatg tgtgagactt
      781 ttagaagtta tttacgtcta ttattcagat tttttatttc gattgaatac cgttttatct
      841 ataaataaga tatgtactga ctcatacaaa gggggccttt tcttctccat gtactatcac
      901 ttttcctgat acaactggtt tcctgttacg ttgtagttgg atctgctttt agtataatac
      961 tttattcatt ccaccagact gatagcagtt tgagcagtct aaataatcat gtagtattac
     1021 aggccattct gtttcatacc ttgaagtgct taaaacttca gctaatggcc cattgtgtta
     1081 actcttactc cattcttttt tagtaagctc ccatatatac tgtaaagcct tctatatatt
     1141 atgtcatgtt gctttttaac atttactatg tagttttgat aaaatgattt gaataacagt
     1201 gatggacaat tcttttttat ttttaacatt attttgataa taatgattat ttatttaaaa
     1261 atacattaga taacatttgt tacgacacga aaaagcttca caaacaaata tcgttatttt
     1321 aaaatgaggg cgaactcaca caattaggag gaatagtatg ccatttattg tttacttttt
     1381 tctttcatta ctcgtgatct atattccatt accaacattt ttcctgctcg tcaatcgtct
     1441 agcagagcaa catgatacga ttgctgctct tacgaaactt tgtttacttc tatttgtttg
     1501 tctagattac tttacgagct attattggct aaaacagaca agagtaaaaa agagatatta
     1561 tttcttcctt gtctttttca atctctttga gtttctgatc cattgctttt tacttcatca
     1621 gtatcatacg atccatttgt ggatcatctg ggttttcaaa gtggtcgtat tattttgtat
     1681 gttcaccttt cctatctcaa gaaaatttag ggaggcattg ttttattaaa gaagcttctc
     1741 tgtatcaata aagacacatt taaggaaaaa cttttaaaaa tctaattggg aatttgcccg
     1801 cataaataag aggctgtgac aaaagtcttg tcacagcctc ttattccgta taaacagtgt
     1861 tcaaaagctt gcatcttcga taatatgccc aaccaaatga aaaatatgag aagctatttt
     1921 tgtttggctg ttcttattac ttgatgaaga acagcttttt cacgacctct ttttaaaagt
     1981 ctttttacag ttttcgagca aagaatgata cgattagtac taaaatgatg gcaccaatca
     2041 atgatggcac gattgccatt cctgcaatac ttggtcccca agaacctaat aatgcttgac
     2101 caagtgctga tccaactaaa cctgctacga catttgcaat aatccccatc gaagaacctt
     2161 tattcgtgat tgctcctgca attgctccga taattcctcc gacgataagt gaccaaataa
     2221 atcccatgtt tcgttcctcc taatgtttta cttccttatt actgaacccg tggttctgta
     2281 ttttctttta ctttttgtgc gccgcttcca gctgctgatt ttactttatc tgtttgattc
     2341 gaagcaaatt caccggtttt tttcgctgcg tcagttactt tgtcttgcac cgtttcttga
     2401 tctttttcaa attcttcttt tgttttgatg tccactacgt tagcgttgac ttcgatcact
     2461 tctaaatgtg tcatattttt tacttctttg ccgattactt cttgcatttc ttcaaagatt
     2521 ttttcgatat ctttgccata ttccacaatg acatccatat caactgctac ttgtttttta
     2581 ccgacttctg tttcgatccc agccgttaca ttatctgtat tcacaagttt tcctgccaca
     2641 ttagagaaga aaccaccgtt tgcagataat aaaccgtcta cttgttcaat tgcgattccg
     2701 atgatttttt gcacaacttt atcttcataa ttcagctcac cgcgcattga atcttcctgt
     2761 tcagaatcat gctttccttg gttatttgta tgatcagaac caagttttcc ttgtccgttt
     2821 gtgtggtcag aactaagttt ttcttgtgct ttcgcatggt cagaaggatg tttttgctct
     2881 tccacaggtt tagaaggatc gattgcatca tgttgttctg gaactttaga ttctggagtt
     2941 cctggatgat gaggtgcatg cttttctgca tcatgtttct tctcttctac tccttgttga
     3001 acgttaggga ctactggatc tggatgtgca gatactggtg ctgttttgtt cttttcatcg
     3061 ttcacaccat ttgattggtt tggtgtcgtt gggtctggat gtgcagacac tggtttttgg
     3121 ttgttcattt tattgttatt atttttgtta ttgttattca tggatgtttc ctcctaagat
     3181 ttaaaatagt gatctaagat tttcgtttgt ttcagataaa aaccgatagc gacgccgatc
     3241 gctgccatga tcaagatcaa taaggtcttg aaaaagccca cggtgatcca taaaacggct
     3301 aatatcaatc caagtacccc aaagatgata gggagcttgt atgtcgaaaa tagttctttc
     3361 atttttggtc cccccctatt ctactcgttt gcggtcattg cttcgtttag ctggttcttc
     3421 aaagcctgtg tatttcaccg atactttcac tggctctttt gttcccaata tgtgttgtac
     3481 ttcattttcg atttctttca tcaatgttcc tgttttacca atgagtgctg aagttcgttt
     3541 caactcccct tttacattga cggcgatctt gtttttcgtt gctttgacat ttacttttgg
     3601 tgaatggatg aattcatctt catgcaagtg agaacgaacc attccttcaa ttgctttttt
     3661 atccaacgtc aactccccat caccatgttt cagtagaaag tttccgattg tttttggata
     3721 aaacaggaaa acaagtaacg tgataattgt caaaatagca aatgcaacgg caagccaaaa
     3781 tacatattgt tgcacgtatg aattagtgat ggaaaagaat cgaaacttct ctagctgaaa
     3841 aggcaatgga taggttgcac ttaatagtat cgcaaacaaa agcacagata gcaaaagtaa
     3901 tgttccgatc actccgatcg tttttacacc tttattcatt ttcgcacctc ctcatagggg
     3961 tgaaacgatc aacttcttat aagttgatcg cttctccacc agttactccg taaatttggg
     4021 ctgtaacata acttgcttta ttcgatgcta agaaaacata aactggtgct aattctcctg
     4081 gctgacctgc acgttctagt aaactttgct gaccaaattc agggatcgaa tcagaaggtt
     4141 gtccttcgtc tagttgtaat ggtgtccaga ttggaccagg tgctacacca tttacacgga
     4201 ttcctttagg tgcaagctgt tttgctaaac cgatcgtaaa gttagcgatt gaagcttttg
     4261 ttgctgcgta atctaataaa tgttcgcttg gattgaatgc ttgaacagaa gttgttgtta
     4321 cgattgctcc tcctgctggg atatgcggaa cagcttcttt cactaaagca aacatagcga
     4381 tgatgtttat cataaatgtg tctttgactt gctcaagtgg taactcagca atagactgac
     4441 aagaaatttg ctgtcctgcg tttagtacca tcgtatctag cccgccaagt tcactcacgg
     4501 ctttatctat gatttcttta ggtgcttgtt cgtcacgaag atcaaaaggt agtaagacaa
     4561 tcttacgtcc tgcaactttc acgtattttg ctacttcctc tgcatctttt tcttctcctg
     4621 ggaagaattg aagagcgata tctgcccctt ctctagcaaa tgcaatcgct gctgcacgac
     4681 cgatccctga atctccacca gtgattagta cacggcggtt ttctagttga ttattgcctt
     4741 cataggattc ttctccacaa tcaggaacag gaatcatatt ctgctgtaaa gctggtgtat
     4801 cctgatcttg tttaggaaat ttctccgtat ggtacaagtt tcgcgggtcg attaattttg
     4861 gttctgtcat tgctgtattc ctcctagttt ttaaattgta ttgccaaaac aaatttcttt
     4921 gtttgatacc tgttttaaat agattcttta atttgtataa tctttccggt ataaatggta
     4981 acctaaaact actgctgcta tcaaagagcc aaagactacg agtataaagc ctagagcaga
     5041 acttttaccc ggtaatcctc ctgtattcat tccccaaatc cctgcgaaca acgccggaac
     5101 ggaaatgatt aatgcagctg aatctagata tttcatgata tgatttagat tattgtccat
     5161 catcccgttg aatagatcac caatactctc taataaatct cgatagatct cgatcatttt
     5221 ttctgtttgt cgctgtctca aatggacatc atagagtagc tgttcatcgt ctaaacgttt
     5281 gatgaaagtt tcagaattcc ataattgatc cattgtttcc cgttgatctc ttaaggtatg
     5341 atcgatatat acgatgtctc tttcaagatc tgcctgttta tacagttctt cattttccgt
     5401 tgttttacga gctgctttat ccaaagcgtc aattcgcttt ttcatctctc ttagttcttt
     5461 cacgtaatgg gtgtaaatat ctaataatgt atacgtgatg atcttttcaa acgagtttaa
     5521 atgatttcca tgctttgtca gcagccgatc cacaaattcg gtttttctat cgatacaagt
     5581 aatcaagaga ttatcggaaa gtacaaaaga tattggcgtt aatttttttt cgatacgatt
     5641 atctgactga tctagattaa tcactaccaa agaaaatgga tgctctagtt tcgtctcata
     5701 aaggcgctcc atccgagaaa cttcttccgg atattcggtt cctacgaaaa tatcatcagg
     5761 aagctcgaat tcttcgattg cttgctcaat ctcttcgggc tttgcactat caagtacgag
     5821 ccaattatat tttttttcat ctgtttctat tacattcttt tcttcatcca caaagtagta
     5881 tctcaatgta tacctccttt ttggactttc tcgaaactat tagctgaaga agcagttctt
     5941 taatttgata ttataatcga gtactgcgat ttcattttct aatgaaaagc tgtcaagctg
     6001 tttgttgaag tccatgttca atcgtttacc gaaataattt tgctctaatt tagatgtata
     6061 gatgtccaat aatggatgct cgattttagt cgcatattct ccaaattccg aaacaaaagg
     6121 aatgatttga aaaaagctgc cttttgcaaa agcacctttg tcccttagaa atggatcgga
     6181 atcattgata agcgcgagtg cgatttttgt attttcccca accttttcaa ttaacgtctc
     6241 tactgcattt ttagacattg taatttccat tcctactccc cctcttcagt tgttgtcttc
     6301 tattatttaa caactacatc tgtttttggt tgaacttttg catattttgg atatttaggg
     6361 aatttagcta cacaacggaa agccgtcatc cctgcatatt tttcgtaata tttcttcgtt
     6421 ggcccttcaa aatccttgct ccacggtttc acatgtcctg aaaaatggat aatgtaagga
     6481 ttgttgcgag tttcttcgta ttcacgttca cctgctgctg ttggatgttt ttttgctttt
     6541 gccatgatat agccttgtgc attccaacgc ggatgaagtg gtaaccaacg gtcatgtaag
     6601 atagcattta atgcatcttg gtcatggaat cgtaattttt ctggattatg ttcgatgaaa
     6661 tctaatactt tttgcgtaat attttcatct aaccattttt tcacattgat cagcatcaaa
     6721 cctgagttga aataacgcat tgatttagct gaaattttca ttttttccag tcgttgatgg
     6781 aacccagcgt cttcaacagc tgctacgatt gaatcaccga agtcaagacg ccataattta
     6841 gagatatctt gtaaagcgat catgtcgcaa tccatatata agatacgttc aacttctgtg
     6901 ccgcggaaca attccggaat cgcaatacga taataagctg tttctggaat acgatcgctg
     6961 attaccacat ttgtaaagaa ttctttattg attttcaaga attccacatc tgagttcatt
     7021 cgtgcgtttt tcactgagaa gcgtaaaagc tgtttacttt caaaatcgat atcatcgtca
     7081 atcacataga accgaacaaa tttagacggg ttgcaattat ctaatactga gacaaataat
     7141 gcagctaaat gtggtacgaa ttttgtgtta caacttgcga ctactgctat ttctttttta
     7201 ttcattgatc tcgcctactt tcgttagagt actgtgtgct aattttttta agtataaatt
     7261 ggtatacgga tgatctttca acgtattcca aggtttatca tgtcctgtaa agtgaacgat
     7321 cgaaggtttt tcatttcctt ctttatactg acgttcatat ttttcatttg gtgctggatg
     7381 acgttcaaaa atcagcgaag tctgcatgtt ccattttgga tgaagttgtt cccaatcttc
     7441 ataaagtaca gcattcaaag catcttggtc gtgatagatg attcgatcgc cattttcact
     7501 gaggtaatgg atagtttttt ctgtaatttc ttttttattc cattgatcaa tgtcaatcac
     7561 catcactcct gaattgaagt acaagtcatc ggaatcgatt cctaatcttt ccaaagcttt
     7621 cacttggcct ggatcgataa tagcgccgat tgtttttcca tttaacggct catcatatag
     7681 ttttacgata tcgtctagta ctaacacatc tgaatctaag taaaggactt tcttataatc
     7741 ctctttagct aataagtttg gtaaagaaat tcttaagtaa gcggtagttg tgatatggtc
     7801 actgactagg aagtcttcgt agatatcttt ttccactgtc aggaattgga tacttgcatt
     7861 acttgaatat ttatttaccg tttcttccaa tccttcttta ctatattctg atagaccatc
     7921 gtcaatcaca taaaatttta tgcgtcttgt tttattgcaa ttttctaatg cagttgcgat
     7981 catcacgctc aagtaaggag cataattttc gtctgaagca gttaccacag gaactacgcc
     8041 gtatctcttc tccatcaaaa tcactccttt ttctaatctt tttctaacct gttctacatc
     8101 tagaatattc ctcatttggt tttaaagcaa aatatatgct ttgttttcat tgaatatttt
     8161 aaaatttttt tatcacaaaa cgcatcggta agcgctaact attcgttttt catggctttt
     8221 tattcaaagg tattga
//
LOCUS       DAHUVV010000111         7535 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.114, whole genome shotgun sequence.
ACCESSION   DAHUVV010000111 DAHUVV010000000
VERSION     DAHUVV010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.114"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            15..1415
                     /locus_tag="NK767_002480"
     CDS             15..1415
                     /locus_tag="NK767_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCH6695431.1"
                     /translation="MLRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLNDINNIKKEF
                     EEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDEPDYLQLTEC
                     EFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRLGGFNSWELP
                     ESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTIDSKFIEEIKK
                     RPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDFQQLHQVFID
                     NTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQTN
                     LYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYIVTNSDVHYL
                     IYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIPI
                     SINTIDRAIISAVQNK"
     gene            complement(1507..1872)
                     /locus_tag="NK767_002481"
     CDS             complement(1507..1872)
                     /locus_tag="NK767_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCH6695432.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1869..2234)
                     /locus_tag="NK767_002482"
     CDS             complement(1869..2234)
                     /locus_tag="NK767_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCH6695433.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2426..2674)
                     /locus_tag="NK767_002483"
     CDS             complement(2426..2674)
                     /locus_tag="NK767_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695434.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2673..2801
                     /locus_tag="NK767_002484"
     CDS             2673..2801
                     /locus_tag="NK767_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HCH6695435.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2858..4552
                     /locus_tag="NK767_002485"
     CDS             2858..4552
                     /locus_tag="NK767_002485"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCH6695436.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4556..4675
                     /locus_tag="NK767_002486"
                     /pseudo
     CDS             4556..4675
                     /locus_tag="NK767_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            5203..6336
                     /locus_tag="NK767_002487"
     CDS             5203..6336
                     /locus_tag="NK767_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCH6695437.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6367..6687
                     /locus_tag="NK767_002488"
     CDS             6367..6687
                     /locus_tag="NK767_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HCH6695438.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6716..7180)
                     /locus_tag="NK767_002489"
     CDS             complement(6716..7180)
                     /locus_tag="NK767_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6695439.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7312..>7535)
                     /locus_tag="NK767_002490"
     CDS             complement(7312..>7535)
                     /locus_tag="NK767_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6695440.1"
                     /translation="VSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAA
                     VNLVLSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         2459 a         1456 c         1358 g         2262 t
ORIGIN      
        1 atattgagaa aaatatttta agacagctgt ttttatgtgg gcaattctat gtaaataaag
       61 aagtcaatct agaaaagctc tccaatctcc tgcatgtttg caaaaccact ttactcaatg
      121 atatcaataa cataaaaaaa gaatttgaag aacagatcgc ttatacacat cgggaaaagg
      181 accgttacac tttatatttt tcagaacata tacctcgatg caagatcatg cagcaattgt
      241 ctcaaaattc gttgttttta aaaacttgtc tgctttattt ggaagaagat gaacctgatt
      301 atcttcaatt aactgaatgt gaattcatct ctgtcagcaa agcttattca ttaaaaaaac
      361 aagtattagc ttattttaat gactgcggga tcgaaatcga ccgctactcc cctcgattca
      421 ctgaaatgga aagacgtctg ctgcttctta atgtgtcgta tcgtttaggc ggttttaatt
      481 cttgggagct acctgaatcg ttttttgaac gtgccgatcg tttcattgaa tccgtaacgg
      541 aaaatagtgg cagattttat gataaagaaa ataaagaaat cttatcgatt ggttttgcta
      601 tcagtttcct acgccagcaa gtgtgtgctg tgacaataga ttcaaaattt atcgaagaaa
      661 tcaaaaaacg tcctatttac aattatgtcg aatcagcatg ggaaaacact gatttccaaa
      721 cctattataa aaaagaagaa ttcgccttta ttttgacact attcaatctt tgtaattatg
      781 gtttccattc ttatcagttg attgcggaag actttcaaca gctacaccaa gtgtttattg
      841 acaatactcc cgaaataaaa gaactagttg ctacctttga atctcacttc aatcaagaat
      901 tattcggaaa ccaaccgttc gaacgtgcgc tgattcatct gatgcgttcg gcttgggata
      961 actatcaatt attcatgcca gaaaaatttt atcttttaaa tgaggaacag acaaatttat
     1021 ataaagaagt ccagacaatt ttttccagct ggtccagcca gttaccttat gatctacggt
     1081 tgaatcctaa ttgcatgcgg gcttttgtga tcgaattgag cggaattctc cgtctgacaa
     1141 aagaacatct gactatctat atcgtcacaa attcggatgt tcattatctg atttatcggg
     1201 aagccttgga agctgtaact actttcgatt ttcaagttgc accaacaatt tattcaagta
     1261 tatctgatat aaagaagtat gcgcagcagt cctcaaaccg ggtattatgt gaacgtacat
     1321 tatatacacc agatgcggtc caatatgaaa atattattcc tatttcgatc aatacgatcg
     1381 atcgagctat catttcagct gtgcaaaaca aataaggggc tgtgacaaaa atcatgtcac
     1441 agcctcttat ttgttgaaga tgcccatttc tgtaacaatc tcttgataca cgtcaggctc
     1501 acaacctcac ttattttgga catctacggg tccgatctct ttatttaaaa aataagaaat
     1561 gatggttcgg ataactacca acgccgctag ttggaaaata tcctgcaagg taggtttgat
     1621 aatcgattcg atgatcccag ctgaaacaag aatttccaaa cttagaagta tatagcctcc
     1681 caacatcttt ttaatagcat tgttctcgct gactcggtcg ctttgcactt ttgtatggac
     1741 aaataaatat ttcaaaaaat ccttgattga tagacaaaca ccccaaacca ataaacagat
     1801 agcaaagata tcaagtccca gtacaagttt ttcgaaatat ggaagaagaa actgcatcaa
     1861 ctctgtcgtc atcctacctc tcctcttctt tgtttttctt tgtcttctat ttcctgattc
     1921 aagaaataag aaatgactgt tcgaatgata ataataagtg caagcttcaa aatatctgtc
     1981 caagtcggct tgataactga ctcgatgata tcagcaacga ctagtacttc caagcttaac
     2041 aaaatataac ttgctagcag ttttcttgta tctgcattac gagtattacg ttctgcatag
     2101 tttttcttga catgagacca atcaaaaagt ttgaatagaa caagaacgat cccgacaatt
     2161 aagataccga tagaacaaat atttaaaata gcaatgatcc attctagaaa ttcaattaaa
     2221 tttgagcgaa acatatacgc tacctacttt cttttaattt gttcctattc ttttattgta
     2281 aaagaaagtt accttgttcg tctatttaaa agttgttcta atgataacgg ttcgcttaaa
     2341 tttttctttt tcactctccc atttttctcc ctccatccat aaatcgtgga agtatccaaa
     2401 acaacgtagc aagttaaaat aaggcttagt ccgtcatcag cttttcaaag aagcactgtc
     2461 tcggtgcttt ttttgtaccc aaatttctaa aaatagaacc atacacctcc tcaaatcttt
     2521 tcttcttacc taccctattt ttatttacag gaaaatcaga cacttcttct tttttgtcaa
     2581 aatttccttt ttcttctcgt cctatcattt ggcattctaa taaaaacgaa ctatccttgg
     2641 atatagaaag agaaaacaag gaggcgtttt tcatgagtaa aaaattaatc aaagtcggca
     2701 tcggtttagg tttgcttgcc cttggcgctg catatctagg aaaaaagaca ggactattcg
     2761 aagatgacag tcatttatat gatgaattcg aatcaatcta gcatcctatt tagttgaatc
     2821 actacggaca tacttggaag acattggagg aatcacaatg agaaaaagta aagcaataat
     2881 gattggtgcc ggtattgcca atatggcagc ggctgtttat ttgattcaag aaggcaaatg
     2941 gaaaggggat cagatcacct tttattcatt ggatgatcac ggttcaaatg atggtgcacc
     3001 gaccgacact gtagtggatg aatattggaa taaaaaccat ccattagaaa accaaaaagg
     3061 ctatattgct cgaggcggtc gcatgcttaa ttaccgtacc tatgtagatt tgatggatct
     3121 gttaagtcga ataccttctg ttactgagcc agggatgact gctgaagaag atacgagaga
     3181 ttttgatgca aaacaccgaa cctttgacaa agcacgtttg atggaaggtg gcaaaggaat
     3241 cattgatggc ggaaaactgg gattcaataa taaagaccgt attcttctga ctaaactgat
     3301 tgctatgcct gattcagaag aagaaaaact agacaatatc actatcgaag aatatttcaa
     3361 agatgatcca cacttttttg aaacaaactt ctggtttatg tgggaaacaa catttgcttt
     3421 cagaacacgt agttctgctc aagaattgcg tcgctatatg catcaaatga tttatgaatt
     3481 cactcaaatc gaacatctag tgggcgtcaa tcgaacacgt tacaatcaat acgaaagtat
     3541 tatgcttcct ttgatcgagt acttgaaaga acaaggatgc aaaatcattt tgaaccgccg
     3601 tgtcactgac tgggaattca aagaaacacc gatgcaagat gaaatcactg tgactggttt
     3661 gaaaattacc aatacactga ctcaagaaga agaatacgtt gtggtcgatg aagatacagc
     3721 agttattttc actaatgggt ccattacaga ttcagctact ttaggagatt acgaaacacc
     3781 agctgtagaa aatatggatt atggtgcagc ttctagtctc tggaaaaaag ccagtgaacg
     3841 tttctacaac ttgggaaatc ctgacaaatt ctttgctgat cgagatgcaa gcgaatgggt
     3901 cagctttacc ctgacgacga aaaaccattt gttgttgaat gaaatcaccc gtatcacgac
     3961 tcaagaacca ggaaatgcct tgaattcatt tatttcaacc acaccgatca cgccgttagg
     4021 ccaaaaagat gtcaatatgt ctattgtcgt gcatcatcaa ccgcatttta cgtctcagaa
     4081 accaaatgaa acagttattt ggggttattt cctttatcca agacgacgtg gcgaatttgt
     4141 ggataaacag tatattaaaa tgaatggaaa agaaatgcta gaagaactga ttggacaact
     4201 ttcaaaagta gatcctggac cagtgaatat tcgtgaaaaa gaaacagaaa ttttcgacag
     4261 cgtaataaat aatatccctg tctatatgcc ttatgcttcc gcgttgttca ataatcgtgc
     4321 caaaagcgat cgaccaaaag tcattcctaa acattcgacc aatctggcct ttacaggaga
     4381 atttgtcgag cagccttatc agatgatctt tactgaacaa agtgctgtac gttctggaga
     4441 aatcgctgct ttccattttg cgggtattcc tatgtcccgt cttgtcaaaa caccgcgtta
     4501 cgacaaagat attcctactc tagctcgtgc cgcaaaaaaa atgtttgaat aatcaagagg
     4561 ttgtgaaaaa gctgccctgc atcaagtaat aagaacaacc aggctaaaac agcttcttat
     4621 gtttttgatt tggttggact tattaccgca gatgcaagct tttgaacacc gtttaccaag
     4681 aggttatgaa aaagctgccc tgcatcaagt aataagagca gccaagctaa aacagcttct
     4741 tatgtttttg acttggttag acttattacc gcagatgcaa gcttttgaac accgtttacc
     4801 aagaggttat gaaaaaggcg tttagtttcg agtatgaaga aaaattttgt gaaaatggtt
     4861 cctcacgttt ttgcataaat ttgacttaat accgagaaac tgatcccttc tgtcataacc
     4921 tctttttatt tatgtctgtt tctttttgaa aacaactatt ttttcaacgt ttactctctt
     4981 gccaatccaa catcatctaa cggatacaac tatttcaatg attaccatta cactaaaaaa
     5041 tcggaaacac acctctccga acacaaatac ataagactat agaatgtttc atttaagaaa
     5101 aaaagcgttt cattatcaaa aaggtaagat ctcctttaaa atgaaattat ctgtagtact
     5161 aataatgcag acaattttat gaatctgaaa ggagcatctc caatgaaagc agtcacttgg
     5221 caaggaaaac agaaaatgga aatcagaaac gtagatgatc caaaaatatt agaaccaaca
     5281 gatgcaatca tccgtatcac agcaacagcc atttgcggct ccgatctgca tctttaccat
     5341 catggtgaag cagttttgga aaaagggtat gttgtaggac acgaaccaat gggaatcgtt
     5401 gaagaagtgg gatctcaagt tcaaacattg aaaaaagggc aacgtgtggt tatccctttt
     5461 aatattagtt gtggtcattg tcatttctgc ctgaatcaca tggaaagcca atgtgatgaa
     5521 tcaaatccag atcagttata tggcgggctt ttcggatttg gcaagttgaa cggtaatcat
     5581 tggggtggtc aagcggaata tctaagagtt ccttatgcag actctacttc atttgttgta
     5641 ccagataatg atcttcctga tgaaaaagtt ttatttctat cagatatttt gcccacagct
     5701 tattggagcg ttgaaaacgc aggagtaaaa aaaggcgata cagttgtgat tctaggttct
     5761 ggtccagtcg gtctattcgc tcaaaaattc gctgttatgg ctggagcaga gcgcgtaatc
     5821 gcagtagatc cagtacagca ccgtctagat aaagcggcta attacaatgg ggttgaaact
     5881 tatttcttag aagacacggc acaagctgga acagaattgt atgaactggc taaaggtggc
     5941 gcagatgtca ttatcgattg tgtaggaatg gacggattag agcctgtcaa agaaaaagcg
     6001 aaaaatttag taagcttaca aagcggcaca atctctccat tacagatggc atcgcaagct
     6061 gtcaaaaaat ttggaacagt ccaagtaact ggtgtttata tgactccagc ttcttcttac
     6121 ccgctgcaag aattttttat gcgggatatc gctgtcaagc atggacaggc accaattatc
     6181 catctaatgc caaaaattta cgcaatgatt gcagaaggac tattcgatcc tacacaaatc
     6241 attactcatt cgctgccact atcagaagca gcacaagcat atgagatctt tgacaaaaaa
     6301 gaagataaaa atataaaagt tgttttgaaa ccttgaattc agtaaaaaat acaggtgatt
     6361 ttctttatgc ccaaaatcaa acgaaatacc catgagatcg aaataggaaa agaagaagat
     6421 atcgaaatac gaggacgtcg cttccgcttc tatttccaaa atcgttacac attgatctca
     6481 ctggctgtcg atttattaac cgggattttc tatatttttg gaagccttgc ttccttaact
     6541 aaaatccccg atcgatatgg cacctatttt tatttggctg gtgctatttt cttgacgatt
     6601 cgtcctattc tcagaatcgt aaggaacatt tttatttacg acgaaaaaaa agcaatgaag
     6661 agaacaagca aaaaaaaaga ggcatgagaa tttttcaaat ctcatgcctc ttttttcacc
     6721 ctatcgtatc actttgatac agacgagcgg tcgtcccact ttcttccttg accattgtca
     6781 aaaatgtcca ctcgtatcgt tcataaaacg tggtatgatc tgtaattaag taaagttttt
     6841 cgtacccaag ttgatgcatt tcttttctga tttccgctaa tatccgcttg gctatcccct
     6901 gcttacgctt ttccggttca acaaacaggg cacaaagatt tggtgtcaga tcagggcggt
     6961 tatggaagtc gttttcaatg acgcccgctc ctccgacgat ggtttctttt tcatctaaga
     7021 ccacatacca ttgaggaata ctttccttat tagcgattcc agtacgaata ctttcctcat
     7081 acacaacagc aggaactccc cattttgatg aaaaccaatt cgcgctttgt tttactaatt
     7141 ctgggtgttc tcttaatttg atccagttat atgtaaccaa tgtttaaacc tccgactaaa
     7201 ataaaacgtt tacaaaaatt atacaagtct ttatgaaaat tagcaatgac aacaatagtg
     7261 atgtaaaaaa aaaaacggaa aacaatcact tgttctccgt tttttgatta atcaagcatc
     7321 taaagcttct tttcttcttt cttggatcgt agctaccttg acgaacggta gatagatcaa
     7381 aatagaaaga accaaattga cagcggctag tacaccacct gcaatacttt ttgttgccaa
     7441 tacaccgcca ataattggtg gagttaccca tggaggcata acggtagctg ctggcactaa
     7501 acctgtagaa gtcgcaaagt aagcaacact aacta
//
LOCUS       DAHUVV010000112         7503 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.115, whole genome shotgun sequence.
ACCESSION   DAHUVV010000112 DAHUVV010000000
VERSION     DAHUVV010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.115"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            14..1141
                     /locus_tag="NK767_002491"
     CDS             14..1141
                     /locus_tag="NK767_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6695441.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="NK767_002492"
     CDS             complement(1359..2501)
                     /locus_tag="NK767_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCH6695442.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="NK767_002493"
     CDS             complement(2506..2691)
                     /locus_tag="NK767_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCH6695443.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(3045..3290)
                     /locus_tag="NK767_002494"
     CDS             complement(3045..3290)
                     /locus_tag="NK767_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6695444.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3284..3685)
                     /locus_tag="NK767_002495"
     CDS             complement(3284..3685)
                     /locus_tag="NK767_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCH6695445.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3872..5680)
                     /gene="uidA"
                     /locus_tag="NK767_002496"
     CDS             complement(3872..5680)
                     /gene="uidA"
                     /locus_tag="NK767_002496"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCH6695446.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5703..7469)
                     /locus_tag="NK767_002497"
     CDS             complement(5703..7469)
                     /locus_tag="NK767_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HCH6695447.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
BASE COUNT         2403 a         1438 c         1038 g         2624 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca
     2821 tcgcttttta ttgactataa aaattatttt agaatattta tcgctccttc tttacagcaa
     2881 gaaatcatgg cgcaactaaa atatgctcct taaagtgtta acgtactgat agaatctatt
     2941 taattttcaa tgaacaatag gttttctacc taaaagaatc tactaattcc ttaactatta
     3001 gaagattctt ttaaacagaa aaagtggaaa ggggattttt ttaattagtt aattttaaat
     3061 tttaagatcg cttctattaa tttaatttct agctgactta tcatatattc atctacatag
     3121 gtaacgaatt gacctacctc gttaaatcca tttgtcaaac ataacttagc catataagaa
     3181 tgataatgtt tgagtatcat cttgatcgaa attggatctc cttctagagc agataagata
     3241 atggatagtg gaactaatag gtactgccgc tttttagatt tatctaccat aggtcatacc
     3301 ccattttcaa ttgttcaact gccttctttc gttgatacca gataccactt gtgctcatat
     3361 tgagaatttt accgatttca gcatcattaa aacctgcaaa ataataaagt aggataatct
     3421 ttcttcttat ctccgataac tgatcaattg ctgctgataa cttttgatct gaaattaata
     3481 tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg gaatacctgc
     3541 cttcatttga atctgccaat ggttcctcat tatcctttat gttagcgttt cgttttcgaa
     3601 tagacttagc ttcgtttcgt aatacttttt tacagtaggc atcaaattgg tgttgtatag
     3661 atatgtcatt gtaatgttgt tgcatgataa tatgctcctt tttccccctt tccactaata
     3721 caacaatcag aaagcccctt tttttcgaaa aaactagaaa aaaaacataa aaatatttat
     3781 cattctttaa acagaaaatc tttctataaa aaacaaaaaa aaacgattag gctaggctaa
     3841 tcgttttttg aacattctta aaaaatccaa attatttttt ataactgaaa ttgggtattg
     3901 atgaccatcg tttctgtaga tagtaagcaa tcattttagg ttttctatct ctagtaaaga
     3961 ttcctttctt atttccttgc actcttaaaa tcccttgact tgttgcaaaa tctgcaaaat
     4021 tccacacttg ttcaccaaca aaattttcac atgtatctat tacttcgtga tttgcttcgt
     4081 aataagccac ttgatactct tctgtaaaca ttgttggtac tgtatcatga aatccagcta
     4141 ctgtatctgc tccatattct gtaaacatta ttggcgtatc aggacatctt tccatccatc
     4201 catctagctc ttttttcaac agatattttg catgtgccag atcccctccc gaaacatacc
     4261 aaccataata acgatttaaa cataaaacat cgacaatatc tcctacctta caaacatctg
     4321 gcgttgattt tagataagta acgattgtta caggtctttt ctgcggatca agttctttag
     4381 ttaattcaac taaaggttca aaatattctt tagcaccttc ggaatccgaa tctggttcat
     4441 ttgcaataga ccacattaca acacttgggt ggttcttgtc gcgttctatc aattccttga
     4501 tcaccgtttt atgagaattt tctgttcgta tctctttcca cgtattatgt ttttccacat
     4561 tatcatctaa taaagtggcc atgaaattta aatgtaaccc tactgcaggt acttcatcaa
     4621 taactacaat accttctcga tctgctaatc gcataatttc ttctgagtaa ggataatgtg
     4681 atgttctaaa agaattagca cctatccatt tcattaaatt aaaatcttta atatttacag
     4741 cctcattcaa accacgtcca ttaacatacg aatcctcatg cttaccaaat cctttaaaat
     4801 aaaaaggttt attattaatc aaaaatttgc cattggtaac ctgaacactg cgaataccaa
     4861 attcttctgt ataggtatcg atcactttat tatcatcaat taaactaatt ttcatactat
     4921 ataaataagc gtctagaggt tcccaaaaaa ttggattctt aagctgaatc attcctttct
     4981 ttccttcagc ttcagctaca agttgagcct cttcgtcata tatcgctatt aagatttttc
     5041 cggtccattc tgtatcgata ttgaattcta cacttgcatc caaatcagtt attcttgtat
     5101 taatagtaat atcacttata tagtctttcg gagttgtata taacttaaca ggtctatgaa
     5161 tacctgcata attgaagaaa tcaaaattaa ctaagttttc cttttttata tccccatttt
     5221 cctgtactgt ttccttcata atccccacag gtaaagtact atcgtcaata atattgttta
     5281 cagcaactgt aattcgatta ttcccgtctt ttacaaaccg attaatttca gactcaaatg
     5341 gtgtaaaccc acctttgtgt tgagtaataa actttccatt caaaaaaact tttgcttcat
     5401 gtgttgcaga tccaaaacga agaactaatc tttcttctaa taatctttta ggaagagtca
     5461 cttctttttc ataccacacc catccaacat gatttctaat gttctctgtt tcaaataaat
     5521 cattataaga agagggaact gaaatcggat aactctcttt ttcatttatt ggttctaaag
     5581 caagttcttc ctgtaagtca tcatttgtat ctaatttaaa tctccagaga ccgcttaagt
     5641 caattacttg cctcgtctct gtcataatgg gatacaacat ataaaccact cctatctaaa
     5701 tattaaataa atgtatcctt taaaccacaa taagcatcta ttggacttac tcccttaaat
     5761 tcagaaagac ccattagttt gtccaccaat atttctaaag tttcttgttt tccatcataa
     5821 gtattaatat aagtctttac ttgaggtaca tcagccaaat gaaacggaca ctgtacagat
     5881 acaaaaattg ttggaatttc atgaatgtaa aacggtatat ccggagttcc tttacttgct
     5941 ggccagacaa ttcgttgaac tgtgcctgga ttaacattag ctatattaat gatcaagtca
     6001 tattttttag ttaaattttt aattggtctc ttagcagcat atattccagc tatattttta
     6061 attttttctt cttgcgataa tcctttcaat tcttcttcag gtgaaatgta tttttcaact
     6121 tcaaatcctt ttgctgacaa aatctgtttc aataaatcaa tggcttgagg tctatttcca
     6181 gcaactactt caccaaaccc accttttacc ccatgtacat ctactagtaa aatcctttta
     6241 tatttttctg gtacaatagg ccaaatatct tgtttatctt taactaatgt aattgcttgg
     6301 tcagccacct ctttaaaaat tgatttattt tcaattaaac caattttttc cagagcttct
     6361 tgcttaggta ataaaatttg ttcttttgct tttttgtgta atcccaattt agcttttaac
     6421 cctaaaattc tttttaaggc atctaataac cgatcatcag tgataattcc tttacgatag
     6481 ccttccatca tataattaaa gtcttcatct ggatcattaa aaaatagaaa tagatcacat
     6541 ccagctgcaa tagaagtggg caataattct gatcgtttca ttgccccagt cattcctaac
     6601 atatgactag catcagtaac tactaatcca ttaaatccga gttttcctct aagtaagtca
     6661 gtgattaatt cttttgataa agttgcagga agtaaatctt catctttcag atcaggatta
     6721 aaatgttttg aataagctgg catatggata tgacctgcca taattgaagg taatccagct
     6781 tctattaagc ctgagtatac ttttccaaaa gaattatccc attcttccgt acctaaacta
     6841 ttaatactaa aagatagatg ctgatctcgt tcgtcaatac cgtcaccagg gaaatgttta
     6901 gctgcaggca aaattccact ctcctgaata cctttcatat aagctaaact aagttctaaa
     6961 actttatcag ggttttgtcc aaaagatcga ctagaaatga taggatttct ccaatttcta
     7021 ttaatatcta caataggagc aaaactccag ttacatccaa tagccgatgc ttcaatccca
     7081 gaaactctcc ctaattcata agcatatttc gcatcatttg ttgcaccaat ttttacttct
     7141 acaccaactt cagtaccatc tgtacaagct ccgttaccac ctgcttccgt attagcagca
     7201 attaatagag gaattttact ttctgtttgt aagatatagt tttgatcata tacctcatca
     7261 gcagttcctg gattatatcg tacagctcca atatggtact gattgaccat attactcaag
     7321 taatttttat cacgactaga tcccatatta acaaacaatt gacctatttt ttcttccaaa
     7381 gtcatagaat caatcgtctc ttcaatccaa gaaatagcat cttcatctaa attatatggt
     7441 ttttttcgta aatctacttt tatagtcatt ttctttctcc tctacttatt agtaagattt
     7501 ttc
//
LOCUS       DAHUVV010000113         7397 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.116, whole genome shotgun sequence.
ACCESSION   DAHUVV010000113 DAHUVV010000000
VERSION     DAHUVV010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.116"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..142)
                     /locus_tag="NK767_002498"
     CDS             complement(<1..142)
                     /locus_tag="NK767_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6695448.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="NK767_002499"
     CDS             complement(183..437)
                     /locus_tag="NK767_002499"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCH6695449.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="NK767_002500"
     CDS             complement(548..802)
                     /locus_tag="NK767_002500"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6695450.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="NK767_002501"
     CDS             complement(995..1270)
                     /locus_tag="NK767_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HCH6695451.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="NK767_002502"
     CDS             complement(1242..2195)
                     /locus_tag="NK767_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCH6695452.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2807..4300
                     /locus_tag="NK767_002503"
     CDS             2807..4300
                     /locus_tag="NK767_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HCH6695453.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4435..4731
                     /locus_tag="NK767_002504"
     CDS             4435..4731
                     /locus_tag="NK767_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCH6695454.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4755..5714)
                     /locus_tag="NK767_002505"
     CDS             complement(4755..5714)
                     /locus_tag="NK767_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCH6695455.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5897..6748
                     /locus_tag="NK767_002506"
     CDS             5897..6748
                     /locus_tag="NK767_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695456.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            6769..7052
                     /locus_tag="NK767_002507"
                     /pseudo
     CDS             6769..7052
                     /locus_tag="NK767_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2442 a         1199 c         1330 g         2426 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     2761 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     2821 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     2881 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     2941 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     3001 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     3061 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     3121 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     3181 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     3241 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     3301 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     3361 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     3421 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     3481 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     3541 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     3601 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     3661 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     3721 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     3781 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     3841 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     3901 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3961 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     4021 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     4081 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     4141 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     4201 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     4261 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     4321 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     4381 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     4441 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     4501 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     4561 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     4621 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     4681 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     4741 tttactctta attgtcaaat taagttagac aaatcatcaa tacttacgta actcaaaaat
     4801 acttccaaag gtgttcgata attcagtgat tttctaggaa tattatttcg tttcgatgcg
     4861 acagattgaa taaaagattc atctactgaa ttgaaatcca tagatttcaa taaaccatct
     4921 ctacgtaaca atccgttaga attctcgttt aggcctcttt gagacggcgt tcctggatca
     4981 gcgaaataaa tggcaatatc attggcatta ctgatctgtt tccaatttga aaattccttt
     5041 ccacaatcaa aagtgatgga tttgaataga tttttcggta cagattcaaa ccaatgattt
     5101 aatttgtttt caatatcaat cgcttgtcta ccacacggtt tcaatgtgat gataactttt
     5161 gataatcgct caactaaggt aattacagca cttttatgtt tcaaacctac gatagtgtca
     5221 ccttcaaggt gaccaaactc atttgagaat tggctataat ctttttcacg ttcatgaata
     5281 gagcgacgga aagcttgttt tccacgtctt tcttgatgtc catttggttt acgcttgcct
     5341 ttcatcggta agtcagaagg attaaatagc ccctttttga acatacgata aatggtacga
     5401 gcagaacaac gaataggaaa cgctgcacga ccaacaatca catcgggtgt ccatccttga
     5461 acaacctttc tttgaatata ttctgattgt tcctcaggta aaacaagcgg gcgtctacca
     5521 cagtttgatt tgtttttctt atactgttga tagtactcta aggctgattt tccttgcttg
     5581 aagaacaggt atactttatg gatcgtttgt cttgaacgat tcaagaaatg cgcagttttc
     5641 gcaacagatt gatttatttt gaaataagac tctatcatta caagttcatc cgttgtaaga
     5701 tgggtatagg tcatttgtac tcactcctta taattctttg gacggaacta ttttgagtgt
     5761 agcacaaatg acttttttag ttgtctagct taattttaca atcggcgtac ttaaaaaatt
     5821 ggttcattga gcaacctatc gctgctaaag tagcaaagga aaacgttaaa ggaaagaatt
     5881 tagggaagaa atcgaaatga aatcggacga ctggattgaa cacacctggc ttcctctgaa
     5941 aagaaatagc aaacgaaaga aattttgggt attgcaatta tggccattag gaaaactttg
     6001 tagttacggg gcacgactta ttcttgattt aaccgtttac tcgattggag tatttggaac
     6061 ggcttattac ttgataaatg atcccaatag gattgcagca tcagacattt ttacagtgct
     6121 aatgacgttg atttttgtca tgctattgtc tatttttact caattagtca tgcaatttac
     6181 caggcaaaaa ttaacagcag gaaaactttt caaaagccta ttcgatttaa ttgttgtaaa
     6241 tgcaattcta gcgactttaa taaaacaaat tgaacaatct ggaagaacaa caaaaggagt
     6301 gaaggatttt ttattgacgt ttattcactt tgtaaatgga cacatgtatt tgtttgaagt
     6361 gggtattatt ctcttttttg tcgggtatgt atccacgaaa cttattaaac gttcaattga
     6421 aaaagaaatt gaaattgacc tgattccgac acttcttggt tatgagtaca atgttgattt
     6481 tggaagatat gacgaattga tcaatacgga tatcgttcac acgagtatcc atgaatctac
     6541 tgttaagggc atcagatcta ttttaaaaaa tggccaggaa atgaaagcaa tagatcgaag
     6601 aaaagagatc tacaattttt atgaagtcga agcaagccta cgatatggca tcggaaaatt
     6661 gtggttagat ggaagtaata ttcgtcaaaa caaaccatac attttttgga acagtagtga
     6721 tttagaactt ccaatgaaag ataaataatt atggagaatc gtttgaaaat gattcctcta
     6781 tacaggattg tgcttatagt tggcatggag ttaaaaacct taaagaagga gcagttattc
     6841 aaagtgaaag aacgtcataa tcagttacta tgtagtaaaa cgggtacgat gaacattgaa
     6901 taccgaacag aatatttaga attaacgaat caaactgatt ttttgttgat tcgaaagcaa
     6961 caagagcaag aaaaaaatta atggctactt atagccaatt gattgatgaa tgggaacaat
     7021 tcaaggaatc taaaattcca gaacgtctct aaaagccttt atttgtaaag agcataagga
     7081 aacgtgggaa cactttttaa aaaaagtgtt ccaataggct gtgcctatta cgtttctcaa
     7141 aaataaatgt gggaacaaaa tatttaaaaa taggatagtg aaaggatagt gaaggatagt
     7201 aaaaggaggg tagtaggata gcaaaaagga taggctcctt ttcgctatcc tttttaaatt
     7261 ggaggaatta atatggaagt aagaataaga aatgtggatg agaaaatcgt aagagaaatt
     7321 gatcgaagat gtgacgaacg gttctgttgc aaagttttaa atctactatc aaataaggta
     7381 gaataataga aaaagat
//
LOCUS       DAHUVV010000114         7283 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.117, whole genome shotgun sequence.
ACCESSION   DAHUVV010000114 DAHUVV010000000
VERSION     DAHUVV010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.117"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(170..3319)
                     /locus_tag="NK767_002508"
     CDS             complement(170..3319)
                     /locus_tag="NK767_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCH6695457.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3333..4550)
                     /locus_tag="NK767_002509"
     CDS             complement(3333..4550)
                     /locus_tag="NK767_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCH6695458.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4540..6135)
                     /locus_tag="NK767_002510"
     CDS             complement(4540..6135)
                     /locus_tag="NK767_002510"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCH6695459.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            6260..7045
                     /locus_tag="NK767_002511"
     CDS             6260..7045
                     /locus_tag="NK767_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HCH6695460.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         2132 a         1477 c         1199 g         2475 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcctagtaa ttgatcaata taagtagaat caatatcata caacttagtg gtattttcac
      661 tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc tccatcaatg
      721 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
      781 aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac gattcatacg
      841 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
      901 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc ttgaatcttt
      961 ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct tctgaataca
     1021 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa gtaacaatta
     1081 gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg atcaatcctg
     1141 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtca
     1201 atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg aattccgctc
     1261 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctagtgacc
     1321 aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     1381 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt gggaaatcag
     1441 gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt attaaccaat
     1501 cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata tcaatggaac
     1561 tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat tttgtatagg
     1621 cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct gcttcaaaaa
     1681 cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc atacgattga
     1741 tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     1801 tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct aaaaccgagc
     1861 catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac tgatcatggg
     1921 tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc ggtgtacctg
     1981 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     2041 ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt aatttttgga
     2101 agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc gcatctagtt
     2161 tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga gtttcactta
     2221 actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta ccagaagaaa
     2281 ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga ttatctaaat
     2341 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     2401 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     2461 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa ggatgtaaca
     2521 ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca atcaagcgat
     2581 gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct acctttttat
     2641 tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt aaggcatttg
     2701 caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     2761 tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc tgataaaaac
     2821 catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca ttgatcaata
     2881 aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt gcaatttgat
     2941 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     3001 agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc ccattttcac
     3061 cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat tcggttttga
     3121 tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga ttatttgaga
     3181 tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc ggacgatacg
     3241 tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact tccgcttcct
     3301 cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg caagaagcct
     3361 tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
     3421 tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc
     3481 ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt tgattcttta
     3541 attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa ttccttagat
     3601 aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt ccacatttca
     3661 ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata cttgtcttcc
     3721 tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc tactcttaaa
     3781 accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc attcaaaaaa
     3841 actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg aaaacgaact
     3901 tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt tttcgtttcc
     3961 ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat
     4021 gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt tttaccacta
     4081 tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata cttctgaatt
     4141 ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc attcttgaac
     4201 caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa taaggggaca
     4261 cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc tgaatttttc
     4321 gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc ctcatttttt
     4381 gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt ccaatctgat
     4441 tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg ctcttcccaa
     4501 tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat gtttgaacac
     4561 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
     4621 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4681 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc gtgggatatt
     4741 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4801 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg ataatttatt
     4861 ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     4921 acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga acaagttcgc
     4981 aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac gaatgacccc
     5041 ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag tccctgtttc
     5101 cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5161 tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg accaattagc
     5221 cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5281 cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc catgtaaaat
     5341 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5401 gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg aacccatggt
     5461 tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga tttgtgccat
     5521 catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5581 aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt gacctaaaac
     5641 ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt gttgctcatc
     5701 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5761 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5821 tgcttgatcc gctaagacgt tgaaaagata ctctggtgag atcgaatacc ccaaaatatc
     5881 aacgattgtg gcaatcaggt cttcttttga ttcttcatct gaaagtaatt cacgatacaa
     5941 cattgtttgt ttgcttactg tgtcatattc ctctaaggat tcatctgcta aaagaaccac
     6001 ttgttccaac aaacggtcgg ataaatattt atagaaaatc agtcccaata aataattttt
     6061 atattctgac gcatccattt tactacggag attatctgcc gcactaaata atttttgatt
     6121 taattctgca cccaaaaaga tacactccta tctcttattt aaagttgcta tataccctct
     6181 tattttagca tactcccttt tagaaaaagc aaaaaaaaaa gaaaaaatct tattcttgat
     6241 aacaagctac gtgacgtata tgcctaacaa aaatagaact tttgaatcaa tcttaaatga
     6301 aggtttaacc acagaaccgg catccattat ttttgatgaa gttatcgatg acgttgaaga
     6361 tagtaagtat cataaaaatt taaaagaact tttttatttt gcactcaaca atggaccaag
     6421 tttgttcact ggtatatcag tacctgatag tgataatcca ttaattatgg caaaaattta
     6481 cttaacaaaa tggtgtaata aatacatcag ggataggaat aacccagcat taaaaaaacc
     6541 tcttaaaaca tttggcgaaa aagatgctgc tttaactact agagtagcag caaatgctaa
     6601 tattgatgat caaaaagtat tgaatgacta cttgcgtgga cattttttat atatgagcgc
     6661 tgagaacatg aacggaagta ttttagagga atatttagcc gaagtgttag aacctgaagg
     6721 ttggatttgg tgcgccggct ctgtatatcg agctgttgat ttctgttatt taggaacctc
     6781 accaattctt ttacaagtga aaaataaata caatacagaa agcagttcga gttcagctat
     6841 aagagtagga acaacaatta gaaaatggaa tcgtttaaat aagtcgacaa aaatttctgg
     6901 tttggatagc cctatcccca attggaaagc attaatagaa atgactgaag ctagtaagga
     6961 actggcagct aagttaactg aaaatagtta tttagcttat attaatgaaa aatccacacg
     7021 agaattatgg acactcgatg actaacaata tccttattag tatctatcca cgatacatgg
     7081 aaaaaattat aaacaaacaa aaaaactttg aatttagaaa tttctatctt acctcagatc
     7141 cttataatat cttttggatt tacgaaacaa tccccaccaa agctttaaag tataaattga
     7201 ttgtaaaaaa tccaattact aaacttaaga aaaatcaaca ctacttttta ggagacgatg
     7261 agtttgaaca attagtttaa aag
//
LOCUS       DAHUVV010000115         7278 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.118, whole genome shotgun sequence.
ACCESSION   DAHUVV010000115 DAHUVV010000000
VERSION     DAHUVV010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.118"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(27..779)
                     /locus_tag="NK767_002512"
     CDS             complement(27..779)
                     /locus_tag="NK767_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCH6695461.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1143..3809)
                     /locus_tag="NK767_002513"
     CDS             complement(1143..3809)
                     /locus_tag="NK767_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCH6695462.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4150..5601
                     /gene="cls"
                     /locus_tag="NK767_002514"
     CDS             4150..5601
                     /gene="cls"
                     /locus_tag="NK767_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCH6695463.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(5930..6745)
                     /gene="thiD"
                     /locus_tag="NK767_002515"
     CDS             complement(5930..6745)
                     /gene="thiD"
                     /locus_tag="NK767_002515"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCH6695464.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            7039..>7278
                     /locus_tag="NK767_002516"
     CDS             7039..>7278
                     /locus_tag="NK767_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695465.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFP"
BASE COUNT         2133 a         1520 c         1244 g         2381 t
ORIGIN      
        1 tataataaaa tcataaaaag aacccttcac gagtcgtgaa aggttctttt tttatctgcg
       61 gaccaaagga gcagtctata ggtcagctta gtcgtactaa ggatttctcg tttttcactg
      121 aatagtttgc gaatgtacct ggtcacaaca ggatcttttg cgtacgcgtc atagtatagc
      181 ttcatcaatt cttcagtaat tgattcctgt gtaaaataag taaaatcttt tttcttcact
      241 ttgaatcctt catcaattaa gtatgtttca aattgatcca ttacaactgg cgatgtatta
      301 ggatcttctt ctatcattcc tagttgcttt tcgataggat aaaaaacatc atcgattgat
      361 tccactactc tgattatcac gcaccatttg cttgactttt tcatcaattt ttctaattga
      421 ataagatgta ccaatgctgg atttgctgct gtaaaaacca tttcatatgt ctcatttgat
      481 tggataaatt cttctaacga ctgtttgata aaggaaacat ttggcaactc atttttttct
      541 gcttcttttt ttgcattttc tagcattcct gaagaaaaat cgatcaaatc atagcgatta
      601 atttcttttg ctaaggaaag aaatctgcca gatcctccag caacgtctgc tgctgtttca
      661 gcaggcagga ctttctccgt tttcagataa ttttttacat cttcagcaat agttacttgc
      721 gattcttgct ggatttcata atattcttca gcaaattcat cccattcttt ttcattcatt
      781 tgatttcctc taattttttg ttatatgttt tcacttttcg gaataaatca ttctcctctt
      841 ttatctattc tatctctttt gatgaataat aaaatgattg gctttccgtc tatcataaat
      901 aaaaaaaggg actgtgacca aagtcgtgtc aaagtccctc tttccgaata aacagtgtcc
      961 aatcagatga ccctcagtat tagctcaaaa tgctgatttt acaacttctt ccttaaacgg
     1021 tgttctcctg ccaactcttc ggtcacaagt cacaatattc aacaaacatg agaagctgtt
     1081 tgttgaaatt gcaccttgtg actcagaatt aaacggcagg ctcacaacct cgttgattcg
     1141 ttttatttat ttccttgtac tcgtacaatg atcaatttag tgatttccat caaaacaatt
     1201 gtcacaagtg cgattcccat acatgtaaag acttctgtca atccaaatgc tgatgggata
     1261 gagaagactt ctttcagtcc tgggatcatt gttaagcagt aaagcgcgct acatacaact
     1321 acagctagta agacacttct gttagagaag aaccctgctt ggatagctgt ttgtgtgttt
     1381 gaacgtgctg ggaatgtttg caatgtccga caccaaatca atgttgaaaa tgacatggct
     1441 acaccgaatt caggtgaatg ctgcatgcca agccattgag caaagataac agcaatccca
     1501 atcagcacac cacgatacgt tacagaaatc aatgttttgt ccgtaaagat tcctgaattt
     1561 ggatctcttg gcgggcggtt catcgtagca ggttcagctt tttctgtacc taatgcaatt
     1621 gctggcagtg aatcattgac taaattgatg aacaacagtt gtaatgctgt aaatggattg
     1681 gaccagtcag caactaaggc aaagatgatc gcaatgatcg cacctaaatt tcctgcaaat
     1741 agataagaaa tagctttctt gatattatca aacacattac gtcctacttc tactgcgctg
     1801 acaatagaag caaagttatc atccgtcaat accatcgctg ctgcatcttt tgccacatct
     1861 gtacccgttc ccatagcaat tccgatgtct gcttgtttca atgctggtgc gtcatttaca
     1921 ccatcacctg tcatagctga gatatgacct ttattttgcc aagcacgaac gatacgaatt
     1981 ttattctcag gtgaaacacg tgcataaaca gtgacttttt ctaaaacttc gtttagttga
     2041 tcttctgtca attcatctag ttccgtcccg gtcaaagcca ggtttccttc ttcaaaaatc
     2101 ccgatttgtt ttgcgatagc aacagctgtt gttttgtggt caccagtaat catgattgtt
     2161 ttgattcctg cacttcttgc ttctgttacc gcctgagtca cttcagcacg tggaggatcg
     2221 atcatagcaa gcaaaccaac aaggatcaaa tcgtcttcat cttcaaatga taaatcaaaa
     2281 ttatcaagtg gacggtaagc aaaagctagt acacgtaatg cgcgtgttga aaaagcttca
     2341 ttttgctctt ggaatttttt catcaattca tccgtcatcg gaacgacttc accatttagc
     2401 aatacttttt tactgcgttg gaagacgata tctggtccgc ctttaacaaa caagtaattt
     2461 ttgttatcaa ttgtgtgtcc agtagacatt aattttcgat ctgagtcaaa aggaagttct
     2521 gcttgtcttg ggtagttgtt tcggatttcc tgataaggct ggtttaattt ttcactaaag
     2581 tcgataaacg ctacttccgt agggtcgccc aatttagtac cgtcttcact gatcgatgca
     2641 tcattcgcta aaacactgat ttcgatcaag cgacgttcgt cagctgtcca ttttgaaggg
     2701 tcatttgtga aatccccagt atcgccatta cctaaaaagt agtcaacaac tgtcattttg
     2761 ttttgtgtca atgtaccagt cttatctgta cagatgatac ttgtcgaacc aagtgtttca
     2821 actgctggaa gcttccgaat aatggcatga cgttttgcca ttttattcgt tcccaaagat
     2881 aacacgattg ttacgataga ctgtaatgct tctggaatag cagctacggc tacggctacg
     2941 gcaaacatca ctgcgctgac gatatctgca gtcatatctc ctgtaccttc tccaagataa
     3001 atacgaaaca gttggatacc taaaatcaat aaagacaatg ccaaaatagc caaacttagt
     3061 tttttactga atttatccag acctcgttgt aacggagtga cttgctcagt tgttgattcc
     3121 aaaaggccag caacttgtcc gatctccgta ttattcccag ttgaagtcac taagaattta
     3181 ccgcgtccgt aagttacgat cgttccactg aataccatat ttgtacgatc tccgattggt
     3241 accgtacttg tgatatccgt tacttctttt tctgcagaag tcgactctcc tgtcagcata
     3301 ccttcatcta cttttaatga tcccgcatct agtaaacgtc catcagcagg tacataatcg
     3361 cctgcgtcta acaacacgat atctcctacc acaagttcgt ttgccggaat cgttttttcc
     3421 ttaccatcac gaatcactcg agcatcgggc gctgaaaggt ttttcaacgc ttctaaagaa
     3481 ccttctgctt tttttgtttg gacaacactg attatagaat tcaacatcaa tactgcaaaa
     3541 ataactaaga attcgatgta gtcacccatg accatctgaa tcagtgcgac aatcagcaat
     3601 acgataacca ttgcatcttt gaaagtttct aagaacaatt tccaagttgg cttcttcggc
     3661 ttttctttta gctggttcaa gccatctctt tctaattttt ccttcgcttg cgtggtgctc
     3721 aatccatcgg ttgagctctg cgtttgctgc atcaacgagt cgatcgtttc tttatagacc
     3781 gcttgttgtg cttgttcctt gttttccaaa tttcatcccc cattttttga tcttggccct
     3841 agtcctgtct gaaaaaaaag agacctaaga caaatacgca tttgtcttag gtctcaccat
     3901 ttcatacaat accagaaaac catttggtta acttactgtt gatattatcg caacaaacgt
     3961 tgccggttac tcccctaagg agatattcaa ctgtactaat atcctacctt attaacctac
     4021 aaaaagcaac tactttagcc cgatttacct acattttcat gaaaggagtg aaaagtatat
     4081 tcaccttggt aggcttcttt cttctatcat gctatactaa tgttattgta tgtaaggagg
     4141 aacatttttg tggtatctag tattataacc gccctttatc tattaaatgc acttattgct
     4201 ttggtggcta ttttgatcaa accccgagat gtagcagcca tttgggcatg gctcttagta
     4261 ttgtttgccc ttcctggggt gggctttgta ttatatttat ttttcggacg tggattaacg
     4321 gataagaaaa aattctatct ccgacaaagt gacttgaaag aattagaaaa ctttcagtct
     4381 tttagaggag ataccattga acattacgat cctgacatgg gcgataaaga caaacagcaa
     4441 tttgttgact tcttctcttc attaaatcgt atgccgctga caagaatgaa ttctgtcact
     4501 cttctcacag acggacaaga gaaattggat tcactgcttc aagatctaaa aaaagccaaa
     4561 cattcgatcc atatcgaata ttacgcattt gtgacagata atatcggcca gcaagtctta
     4621 catgttttag aagaaaaagc cgcagaaggc gtggaagttc gaatattata tgatgcattt
     4681 ggctctcatg gcacaaaagc aaaagatttc aatcgtctaa tcaaaaatgg tggacatgtc
     4741 catacatttg ttacctcaca aagggcatta cttcgtttcc gattgaatta ccatgatcac
     4801 cgaaaaatcg ttgtgatcga tggaaagatt agttataccg gtggtttcaa tattgccaat
     4861 caatatgtaa atacaacaaa aaaattcggc tattggcgcg atacgcatat acggattttc
     4921 ggtgccgctt cttctttgct ccagcttcgc ttcttaacag actggaacgt ctcggtacct
     4981 gaagaaaaaa aggtcggcta tcatttgaat tatttcttta aaaaagcaga tcgagatgaa
     5041 tctaagcttg ctgatacatc catccagctt gtttcaagcg gaccgaataa cgaaagggaa
     5101 caaatcaagc tttcatttat caaattgatt acttctgcta aaaaacgtgt ttggatacag
     5161 acaccttacc ttgttcctga tgaaagtgtc attgctgctt taaaaatcgc aactgcctct
     5221 ggtgtagatg tgaaaatcat gattcccaac aaaccggatc atccttttat ttatcgagca
     5281 acacaatatt atgctcggca gctgatcaag gaaaatgtac aaatccttgt ctatgagaac
     5341 ggcttcctcc atgcaaaaac attgataatg gatgatgaaa tctgcatggt aggttcagca
     5401 aatcaagata ttcgtagcta ccgattgaat tttgaaacaa gtgctgtcat ttacgatcct
     5461 gagtttttag aagaacttgc tactcagttc aaagaagatg agacacattg ttcatccatg
     5521 acaactgaaa cagtcaagga aatgtctaac tggctattat tcaagcaaca aatttctcga
     5581 ttattttctc caatcctata atttttttat tcaagtagag aaaaatatac taagattaaa
     5641 gggactgtga caaaaatctt gtcacagtcc cttcttccga acaaacggtg ttctcctgcc
     5701 aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgtttt ttgaacttgt
     5761 tccttgtgac tcggagctgg acggcaggct cacgacctct ttcttaaacg gtgttcaaaa
     5821 gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca taagaagctg tttttacttg
     5881 gctgttctta ttacttgatg cagggcaact ttttcacaac ttctttcttt tactctaatt
     5941 caccagctgc ccaatgattg atggggccat atttgtgccc tacatttatt tcttcacgaa
     6001 ttgcacggtt tacatagtct tttgccaatc ggatcgcttg ttcaactgtt tttcctttcg
     6061 ctaactcagc tgcaatacat gcagatagcg aatcgccagt tccgttgatc cggtcggtag
     6121 gataaaatgg tgcatttaac caaaatgatt ttcctgattc gaacaatacg tagtcattga
     6181 ctgtttcttg ctgcgcattt ttagcatgat gtcctttgat cataacgttt tttgcaccca
     6241 tatcttgcag ttcttttgct gcttgcacaa aatcttcttc agctgctaat gtccgtccag
     6301 tcagcttttc tgcttcataa aaattaggtg tcagtacttc ggccaaaggg acaagctttt
     6361 ctttcaagct ttgcaaagca gattcttcta gtaaaagatt tccatgtttc gttacgatta
     6421 cgggatccac gactaatggt ccaaaatcat atttttggta attttttaca actgtctcaa
     6481 tcaactttga atctgctagc atacctgttt tagctgcacg tatttgaaag tccttcgcaa
     6541 tcagttcaaa ttctttgtcg ataaaatctg ttggcaatgt cacacttgct ccgattccgt
     6601 aagaattacc tgcaacacat gcggtcatga cagaaacacc gtaaacattt cttgtaaaga
     6661 atgtatgcat atctgcttgc atccctgcgc ttccatcgct atctgatcct gcgatcgtca
     6721 ttgcttgtac atactgatct tccatcaaat cgattcctcc atttttataa aaagattttt
     6781 cataatactg ttactgtttc tatcatatgt ttttcaaagt atccctcagt aaatatacca
     6841 ctaccttttc ttggtaatca aatttatttg aaaaaacatt tgcaataaat cagaaaatag
     6901 tggtcgttta tttgtattcg ctattctaat aataaataac cactatttga tttatatata
     6961 tttcagtatc cttcttgttt tcatataacc aaaggcgcta tgatttccat atgaaaaaaa
     7021 taaagtcagg tgaaaaaaat gaaacttgag gataaaaaag tcattttgaa gaaaatcaaa
     7081 cgactaccag accaaataga tggctatacg atctgttctt tcaaaaaaga caggagtata
     7141 aaagcttata cgcaaaacac agaaaaagga ataatctatt tcctacatga agaaggttac
     7201 gatacccaaa cctttatgtt tactgaaaaa aaagaatttc ataaacagat gaaaaaactg
     7261 attgaaaaag aatttcca
//
LOCUS       DAHUVV010000116         7242 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.119, whole genome shotgun sequence.
ACCESSION   DAHUVV010000116 DAHUVV010000000
VERSION     DAHUVV010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.119"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            67..1698
                     /locus_tag="NK767_002517"
     CDS             67..1698
                     /locus_tag="NK767_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCH6695466.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1701..2780
                     /gene="uxuA"
                     /locus_tag="NK767_002518"
     CDS             1701..2780
                     /gene="uxuA"
                     /locus_tag="NK767_002518"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCH6695467.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2854..4281
                     /gene="uxaC"
                     /locus_tag="NK767_002519"
     CDS             2854..4281
                     /gene="uxaC"
                     /locus_tag="NK767_002519"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCH6695468.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4365..5012
                     /locus_tag="NK767_002520"
     CDS             4365..5012
                     /locus_tag="NK767_002520"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HCH6695469.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            5132..6508
                     /locus_tag="NK767_002521"
     CDS             5132..6508
                     /locus_tag="NK767_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6695470.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            6518..>7242
                     /locus_tag="NK767_002522"
     CDS             6518..>7242
                     /locus_tag="NK767_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCH6695471.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKC"
BASE COUNT         2491 a          947 c         1487 g         2317 t
ORIGIN      
        1 cggttacaat gggttttaaa atataaaatg tatcaaaact aacatatcag tttgaggagg
       61 ttaattatgg tttcgattaa aaattggcaa aattctatcg aagaattcaa tgaaatgcag
      121 attctactgc cgtgttttga tgtcaaagaa gttaagcaag aaggccagca acatccagta
      181 tggcttcatt ttggtggagg aaatctttat cgaggatttc atgcagaaat tgctcaatgc
      241 ttagcagatc aaaatgaatt agaaacagga gtagttgtgt gtgaaacatt tgatgaagaa
      301 gtagtggaaa aagcttatca tgactataac aatgacattt tggaaattgt tatgagtgga
      361 gatggtgaac tcaaaaaaag acttttggca gtaacggctg atagtttata ttgcaatccg
      421 aagaatatag cggactatca aagagtggtt aaatattttg aagatcctaa attacagttt
      481 acaacgttta ctattactga aaaaggttac agtttaaaag attctaaagg gcaattcttt
      541 tcggtagtca aagctgatat caaatctgga cctagcaatc ctcaacatac gatgagtatt
      601 gtaacatcct tgttatatag cagatttaat gctggagagt tgccgattgc gatggtttct
      661 actgataatt tctcacagaa tggtcaacgt tttcaagata gtatttttac gattgcagaa
      721 gcttggcaaa aagaaggata tgtttctgaa aaatttattg cttatttaaa ggataaagaa
      781 aaagtgactt ttccttggag tatgattgat cgaattacgc caaatccttc agagaatgta
      841 aaggaagatc tattgatgat ggggctcgag gatttagata tcattcacac aaaaaaacat
      901 accaatattg ctgctttcac taatactgag gtagttcatt atttagttat tgaagatagt
      961 tttccaaatg gccggcctaa attggaaaag gctggtgtga ttatgacaga tagagagacg
     1021 gttgataaag cagacatcat gaaggttacg acaagcttga atccacttca tactgctttg
     1081 gcagtaacag gttgtctgct aggatatcaa tcaatttcag cagagatgca ggatgatgat
     1141 ttggttggtt taatcaaagg aataggttat ttagaaggtt taccagtagt tgaaaatcca
     1201 ggcattattg atccaaaaca atttattgat gaagtaatta ataaacgttt gcctaatccc
     1261 tatattccag atacaccaca acgaattgca acagatactt ctcaaaaagt agcgattcgc
     1321 tatggtgaaa caattaaaaa gtatgtcgaa aaagacggaa atgcagaaaa tctaacattt
     1381 attcctatcg ctattgctgc ttggttacgt tatttactgg cagtcgatga tcaaggggaa
     1441 aattttgtgc caagtcctga tccgttattg gtagagttgc aggataaatt atcagtattg
     1501 aaattaggtg aacaagatcc ttcaaagatt cattgtgcgg tacaggaaat tttagaaaat
     1561 aaagaaatct ttggtttaga tttatatcaa gttggggtag gaaagaaaac tgaaaaaatc
     1621 ttttgcgaaa tgctagtagg tcctcaagca gttcgtaaaa caatcaataa atatataaca
     1681 gaagttggag gaaagtaact atggaaatga cttttagatg gtatggggca aatgaagaaa
     1741 aaatctcact acaagaaatt cgtcagatcc cagcaatgaa aggaattgtt ggaacattaa
     1801 tggatatacc agccggagag gtttggccga gagaacgaat taaatcttta aaagatgaag
     1861 tcgaagccaa tggacttacc ttgaaagtta ttgagagtgt taatgttcat gatgatatca
     1921 aaattggttt accaacacga gatcaataca ttgagaatta taaacagacg attcgcaatt
     1981 tggcagaatt tgggattgag gttatctgtt ataattttat gccagtattt gattgggtaa
     2041 aatcggattt ggattatcgt ttagatgatg ggtcttctac gttagcctat atttcaaaaa
     2101 atatccctac tgatccggag gaaattatta gagaagtaga aaattctact aataattttg
     2161 aattgccggg ttgggaacct catcggttat cacaaattaa gcaattatta gaagcctata
     2221 aagaagttga tgaagagaaa ttacgagaga actttgcata ttttctcaaa gcaattatcc
     2281 cgacatgtga agaagttggg attaaaatgg cagttcaccc agatgatcca ccatattcca
     2341 ttttcggatt acctcgtatt gttaaaaatc gtgacgattt agattggttg tgtaatgtag
     2401 tggattctcc ttctaatggt attacattat gtactggaag tattgcagaa cgaccagata
     2461 atgatgtcta tgaaattcta tctgaatttt gtcaacggga tcggattcct tttgcacatg
     2521 ttcgtaatat caaatattta agtgacaatg gactcgattt ttacgaagcg ccgcataaat
     2581 cagaatatgg ttctctagat atgtataaga tattaaaaac aatgtacgat aatggattta
     2641 atggctatat tcgtccagat catggtcgga tgatttgggg agaaaccggt agacctggtt
     2701 atggattata tgaccgagct ctaggtgcag tttatttaaa tggtatttgg gaagcgcttg
     2761 aaaagggggc gagtaaataa ttatataagg gagtttattt agaggaaaaa ttttctcttc
     2821 taaaaactaa cggaaggaaa ggagtgttta aatatgtttt tagataagaa ctttttatta
     2881 gagaatgaaa tagctaaaag actttttcat aaatttgcaa aacaccaacc aattatcgac
     2941 tatcattgtc atttagaccc agcatatttg ctagaaaata agcaatacaa gaatttgact
     3001 caggtatggt taaatgataa aggagctgga gatcattata aatggcgatt gatgcgagct
     3061 aatggtatag aagaagagtt gatcagtggt ccaggtgatg attatgaaaa atttttagct
     3121 tttgtaaaaa cagtggagaa ggcgccaggt aatcctattt ttgaatggtc tcatttggaa
     3181 ttacaacgtt actttgatat tgatttgaca atttctgaga agaatgcacc agaaatttgg
     3241 cgacgtgcta acgaaaaaat tcaaacggat tcctatcgcc cgaaagaatt gttggagatg
     3301 atggatgtga aagccatctg tacaacggat gatcctacag atgacttgtg gtatcatact
     3361 gagctttcta aacaggataa ttctttcaag gtattaccta ctttccgtcc agataaggtc
     3421 tggagtatca aatcatcagg cttttcgaac tatattttaa aacttagtca aacagcggct
     3481 ataccaatca aagactttaa tgggttgaag caagcattga gccaacgagt ggatcatttt
     3541 catcaaattg gctgtcgatt agcagatcac ggtttgaata cttattttta tgaaatagca
     3601 gatgaaaaaa tactcgacaa tctgttaata aaagctttag caggaaaaac ggatttccat
     3661 ttatcagaaa ttgcacaatt tgttacaggt attcaattac atttgatgtc tgagtataca
     3721 agatatggtt ggacgatgca aatgcattta aatgctttaa gaaataattc aagtatatta
     3781 aaggaacaaa ttggagtaga tgtcggtggt gattcgatgg gggatcaagc tagtttgaca
     3841 caaaatgttg tagcattgtt cgataccgct gaaaagagtg ggtgcttacc aaaaacaatt
     3901 ttatattcat taaatccaac tgattggctt ccactagcaa cagcaatgca aagcttccaa
     3961 gggggtatga ttcaaaaaat ccaattggga tgtgcttggt ggttcaatga tacagctgaa
     4021 ggaatgcgtc aacagttgac tacgatggca caacaaagtt tgttgtcgaa ttttacaggt
     4081 atgttgactg attctcgaag ttttctaagc tatcctcgcc atgagtattt tcgtcgagta
     4141 ttatgtcaat tactaagcga gtgggttaaa gcaggccgaa ttccagatga tgaaatgtat
     4201 cttagtcaga tagtggagaa tatttgttat cgtaatgcgt ataattattt taattttttt
     4261 gaaatcggaa gtagaaaata atacttgaaa taaaagctta aatttattct ttgtaagtta
     4321 gtacagctag acaaagtatg tatccataaa ggaaggagat agatatgaaa aaagtagaaa
     4381 ttttaagtaa gttagaagcg gctggagtca ttgcggttgt acgtgggaaa aataaggatg
     4441 aggccattaa agcaataaat tcaattattg cgggtggaat taaagggatt gaactaactt
     4501 ttacagttcc taatgctgaa gaaataatca aagaaattgc taaaaaatat gctgatcaaa
     4561 aagatgtaat aatcggagcc ggtacggttt tagatgcgtt tactgctcgt atcgcaatta
     4621 tggctggggc agaatatatc gttagtccat cttttgatcg acaaacagct gagatttgta
     4681 atttatatca aattccttat ttaccaggtt gtatgacaat tactgagata aaggaggcac
     4741 ttactgctgg cgtagatatc attaaactat tcccaggttc tgcttttggt ccatccattg
     4801 taaaagccta tcaagctcca ttaccacacg taaatataat gcctactggc ggagtaagta
     4861 tagagaactt agaagattgg tttgaagtag gagttgttgc agttggtgta ggtgggaatt
     4921 tattggcttc tgttgaaaaa ggtgattttg aagaagtaag tcgagtagct agtaaatata
     4981 tagataagtt ttatgagata aagaattatt aggagaaaaa acaaataata ctctgaaatg
     5041 taaaaaataa atactgactc tttacgcttc agagtatagt acgtactttc atcagaatga
     5101 aaacgcttaa caacagaagg gagagaattg tatggaaaga aaatttggta taagagacaa
     5161 aataggatat atgtttggag attttggaaa cgattttact ttcatttttg ctagttcttt
     5221 tttaatgatc ttttatacaa aagtattggg gataagcgga gctgccgttg gaacattatt
     5281 tttagtagca agatttatcg atgcagttac tgatgtaact atggggcgta ttgttgatag
     5341 atcgaaagca actaagcagg gtaagtttaa accgtggatt ttaagaatga gtggtccagt
     5401 cgctttagca agttttttaa tgtatcaaac ttctatgatt tcagcttcta tgagcgttaa
     5461 ggtagtatac atgtatgtta cgtatatatt atgggggagt attttttaca catctataaa
     5521 tattccttat ggatcaatgg catcagctat tacttctatt acagaagaaa gaacaatgct
     5581 atcgacattc cgaaatgttg gagctacttt agcaagtttg gtcattggtg tattaacacc
     5641 attatttatt tatacaagag atgattcagg cgctcaaata gttcgtggtg gatcaacttt
     5701 tacaattgtt gcgggaatat tttcgatatg tgcacttatt tgttatttaa tatgttataa
     5761 actaactact gaacgagtaa gaattgacca atctcaatac aaagtttcat tgttaaaatc
     5821 tttaaaaggg ttagttaaaa atagggcatt actttctata attggtgcgg ctattttctt
     5881 actccttgct caattattaa ttatgagtat gaataattat gtatttcctg attactataa
     5941 tgatgcacag ggaatagttt taatgaattt tatcaatcct attttggttt taatagttgt
     6001 ttcgccttta actttgtttt taagtaaaaa atatggaaaa aaagaactgg catcagtagg
     6061 aatgttcttt tcagcaatag tatatttgat cttatttttg ataaaagcta gtaatatgta
     6121 tgtattctta attcttagtt cggttggcta tatgggtttg ggtgtgttta atactgttat
     6181 ttgggcaaat attactgatg ttattgatga tcaagaagta aagtctgatc aaagagaaga
     6241 tgggaccgta tatgcggtat attcttttgc tagaaaaata ggtcaagctc tagctggagg
     6301 cgtcggaggt tggactcttt ctattatagg ctatgactct ttagcaaatg tacaaacggg
     6361 agatgtttta gaaaagttat ataacgcttc aacattaatt ccatcaattt gttttctgat
     6421 tgtaggatta attttatttt tcctttatcc attatctaaa gataaagtct tagaaaattg
     6481 cgagctttta aaacaaaaac ataaatagga gagaaatatg aataattgga tattttgtat
     6541 tgattcagat ggctgtgtta tggatacgat gacttataaa cacaatctct ttttcggacc
     6601 aattgctgca gagatttttg atgttcatca tagagaaaaa ttcctacagg attggaataa
     6661 tattaattta tattcagaga ctcgtggaat aaacagattt ttaggtttag ttaaaacatt
     6721 agaaaaaaat gatgtaacat cgattaatcg attaaagcac tgggtatctc taacaaaaga
     6781 attgtctaat ccatctttga agagggaaat agaatattct cagtcagagg atttattaaa
     6841 agctttaaaa tggtcggaag aagtcaatca acagattagt caagcaaaag gattagataa
     6901 acccttttta agtgctagag atactataaa agctctgaaa aaatatggga agataattat
     6961 tgtaagctca gctaataagg aagctgttca agaagaatgg gaaagacatg aattattatc
     7021 attagttgat gaactgtgtt gtcaagataa aggaaaaaaa gaagatatta ttcgaagtgt
     7081 aattgaaaat ggttgtgatt tggataagat attaatgatt ggtgattctc ctggtgatct
     7141 agaagctgca aataaaaata aagttttctt ttatcctatt ttggtcaata aagaaaaaga
     7201 atcatgggaa aacttaagaa ctgatgtttt atttaaatgt tt
//
LOCUS       DAHUVV010000117         7088 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.120, whole genome shotgun sequence.
ACCESSION   DAHUVV010000117 DAHUVV010000000
VERSION     DAHUVV010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.120"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(581..1597)
                     /gene="tsaD"
                     /locus_tag="NK767_002523"
     CDS             complement(581..1597)
                     /gene="tsaD"
                     /locus_tag="NK767_002523"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCH6695472.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1594..2058)
                     /gene="rimI"
                     /locus_tag="NK767_002524"
     CDS             complement(1594..2058)
                     /gene="rimI"
                     /locus_tag="NK767_002524"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCH6695473.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2065..2607)
                     /gene="rimI"
                     /locus_tag="NK767_002525"
     CDS             complement(2065..2607)
                     /gene="rimI"
                     /locus_tag="NK767_002525"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCH6695474.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2591..3313)
                     /gene="tsaB"
                     /locus_tag="NK767_002526"
     CDS             complement(2591..3313)
                     /gene="tsaB"
                     /locus_tag="NK767_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCH6695475.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(3504..4484)
                     /locus_tag="NK767_002527"
     CDS             complement(3504..4484)
                     /locus_tag="NK767_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6695476.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4508..5992)
                     /gene="galT"
                     /locus_tag="NK767_002528"
     CDS             complement(4508..5992)
                     /gene="galT"
                     /locus_tag="NK767_002528"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HCH6695477.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6004..6993)
                     /gene="galE"
                     /locus_tag="NK767_002529"
     CDS             complement(6004..6993)
                     /gene="galE"
                     /locus_tag="NK767_002529"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCH6695478.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         1930 a         1551 c         1258 g         2349 t
ORIGIN      
        1 taaatatcag ggtcactgct tattttcctc cctaaaactt ctgaaagatc cggcagtttt
       61 agatcactgg acaagactca aagattttct atgtgtgttt cagttatgta atattcactg
      121 tttgcaaaaa atactgttag attaactaga tgaagcgtct tgtcctcatc cgatctagat
      181 tattcgatat tcctcgacaa taaacgatta ctcagtgagg gcaatcagag gaacggataa
      241 tgataaataa gctgtaaaaa atagcaaaat aacaataatg attaaaagta tgtaaaaaat
      301 agggctttga caaaaacgtg tcacagccct atttcgatta aacggtgtgt acctgtcaac
      361 tcttcggtca caagtcataa tgttcataaa catgaggagc tgttttttga aattgttcct
      421 tgtgaatcag agttaaacga caggcttacg aactcttgat taaacggcgt tcaaaagctt
      481 gcatacgcgg taataagaac agccaggtca aaaacatgag aagccgtttt tcctgggctg
      541 ttcttattac ttgatgcagg gcagcttttt cacaacctct ttattcagat aaaaccaagc
      601 ttggatcagc attcaactga tagctagcaa aatttctttt ttgcatttcc acatgtgctg
      661 ctgctccaat cattgcagca ttatctccac aaagtcgaag agggggaatc actagttcta
      721 cctcagggag tttagttgcc aaagcatcct gcaatccttc acgcaaccct tgattggccg
      781 ctacacctcc agctataatc agttgcttga ctggataatt ttgacaagct cgaagtgttt
      841 tagtaatcaa tacatcgatc acacttgctt gaaagctagc tgctaaatca tttttgtcta
      901 gatcttctcc acgctgttgt gcattatgca ccaaattgat aaatgcactt tttaatccgc
      961 taaaactgaa atcataatta tcttcatgga tcatcgctcg cggaaaatgg taattatctt
     1021 ttccctgatg agctagctga tcgatttctt ttccgcttgg gtaagaaaga cctaatactc
     1081 ttccaacttt gtcataagct tctcctgctg cgtcatctct tgtctcccca atgatttcat
     1141 atgatccatc ttcttgcata tagactaatt ctgtatgtcc tccgctaaca agaagtgcca
     1201 tcaatggaaa ctggaatggt ttaaccagac gggctgcata gatatgccct gccatatggt
     1261 ttacaggaat aagcggcaat tggtgtgccc aagcaaaagc cttagctgca gaaataccaa
     1321 tcagcaacga gccgacaagt cctggtccat aagtgacagc gactgccgat aaatcttctg
     1381 ccgacacacc agcttctacc agtgcatctt ccaagcataa ggtgatttgt tctacgtgat
     1441 ggcggctagc tacttcaggt actacgccac caaaacgttt atgactgttt atttgagaag
     1501 caacgatgtt agataagatc tctgttccat ttctaacgac cgctacactc gtttcatcac
     1561 aacttgattc aatcgccaga atcaattctt cattcatttc atcgtctccg tttttaactt
     1621 tctgctcata atgatcgcat cttccgtcgg attgcgataa tatgatttcc gtttacctaa
     1681 tatacgaaac ttttctgatt cataaagttt tcttgctgct tcattagata gacgtacttc
     1741 taaaaaaaca tgcagcgaat cttttatttg ttccgtaatc agtaattgta acaaatgccg
     1801 tgcatgccct tttccttgtt ctgaaactgc aacagcaata tttgtgatct cgacttcatc
     1861 tattactttc gagtatccaa taaagccaca gatctggtta tcttcagcta aaagcagata
     1921 atcagagtga ggctgttgga tgtctgccaa aaactgcatt ttcgtccaag gtgaaccatg
     1981 tggataagca ttttcgctaa tcatccatac gcattctgcc aagtacgctt catcaaaatc
     2041 tttttttctg caaatcaact aatctcactc attttctaaa agttttatca tatccaacgc
     2101 atcttctttt gtttcagtgt aatatctttt tttgatcgtt gttgcttgaa accccaattt
     2161 ccgataaagt ctttgtgcat cttgattact catacgcact tctaatgaca tcgtctcaca
     2221 gcgattcatt atagcaaagt tctcgatttc gtcaataagc agcgaaccga ttcgtttacg
     2281 ctgatatgcc ggtcgaacgg caatattcgt gatatggcaa tctaaaccga acgctcttga
     2341 accgataaaa ccaatcattc ttccattatc aaagatacct agatatagat gaggaagcgg
     2401 tgacttcaat tctgatataa aggcactttt tgtccatggc aattcgccat tatagacttc
     2461 tcgttcgaca tccagcagct ctttgatatc ttctgtagtc aattctctta gtacgtaacg
     2521 acgatcgatt tgttcgactg ttttttctgt ataaatcatt ttatcttgaa aaaagcttct
     2581 ggcatacgct ctaaattttt tcaacataac tttcgtctcc aggtgtgtgc gtctccaacc
     2641 atttttcttc tgcttctact cgttttaaat agcgaggaag aaaagcttgt ttctctgcaa
     2701 ctggttcaac taaagcacct aattgagcca cagttacacc acttggaata tcccatgctg
     2761 gaactgtatt aatcatcgca ctaggcaata tttcctttat ttcagagaag aatttgtgac
     2821 aatcactccc gacgaaataa atagcttcat tcagcttttg tagttgtgtg aacaaatcag
     2881 aagcagaaat atgtacatct tcaagtacag tttctaatct attttctttc cagcggtatg
     2941 ccccagcgta tacatttttt cttcgagcat caaacatggg cacaataata ttatccacac
     3001 cgatgcaatt agccgcgatg gttttcagac tagatactcc tactagctct ttatctaatg
     3061 tataagcaat cgtttttgct gtcgttacac ctatacgaag ccctgtataa gaaccgggac
     3121 catcagaaac cgcgattcgg tcaatgtctt ttggacttat ttgaagatct gcaaacaatt
     3181 ggtcgattgc aggcatcagt gttacactgt ggttgcgttt gatatttgtt tgtatttgtc
     3241 caagaatttg ttcgtcttct accacaccga tcgccaaagt ttggtttgca gtatctattc
     3301 ctaatgtaat catatatttg atcctttctg ctatgacatt ctcgccttcc attgtagcat
     3361 acttttttat tctgaggatg aaaataccaa aggcaaaact agtacaataa tacatgatta
     3421 tctgctggcg ggcaaatttt gccccttcct gaaacacaaa aaagctgcgg tatcactcca
     3481 ttttcccaca gcttttccat cttttatttc gttgattctc gaataatcaa ctctgtttcc
     3541 acaataatct ttcttgaaac cggtgctttg gaattactta attgaagaat cgtctccact
     3601 gctagctctc ccatccattc ggtataaact tttacagtag aaagcgaggg cgttacatat
     3661 ttcgctacac tgacatcatt gaagcccatc acagaaatat cttctggtac cactaaacct
     3721 ttttcttgaa tcgctttcag tgcgccaatc gctaatgcat cattagctgc aaaaagcgca
     3781 tcaggaacat gcggattcgt ttttaaatac tgcttcactg cttgatatcc tgactcgaca
     3841 gaaaaatctg cttcgataat tggaccttcg tcgtagcctt tttctgccaa aacttgttga
     3901 aatgccataa ttctgggatc ttttagccgc tgatggtttt tcttcgtata ttctgttcca
     3961 gcaagcatag cgatatgttg cgctttatgg gagacaaagt gttcaatgac tttttgaaca
     4021 cttgacttaa agtcgacaac tagagagtcg attccttgag aagtgccgtc agaatctacc
     4081 aaaagcaagg cattttggga tcctttcatt tctaataact gagcttcgtc gaattttcct
     4141 agtgcgatcg ttccatctgc cggaagatca ctaatggaat cccacgtttc tttcaataaa
     4201 gtaacgtttg cttcttctgc ctttttttcg attcctaggc gaatagataa ataataaaga
     4261 tcttcaagct cttccgtatc gtcgtaccac tggatcaggc gaagcgtttg cttagtattt
     4321 gctgcatttt tacgatgttt cgtataattt aagcgttctg ctgcttcaaa aattttttgc
     4381 tttgtggcta agccaacgga aagatcagga tcatagttga gaactcttga aacagtagca
     4441 ggagaaactc ctgccaactg cgcaatgtct ttaattgttg ccatttgtct ctcctccttt
     4501 tttacgttta taaaatcgca gtaaatcgtt caaacgctgc ctgtcctttt tcatctcgct
     4561 tgaatactcc agcgtcttcc agtacacgtg caaaaacctg acctacagat ttttgtacga
     4621 catcggtgac cgtatctttc gtcaaatcag gatgttcttt ttttaattgt tctgcccacg
     4681 gacgatgata ttcagccatc tggttttctt ctcctaataa gaaatgctct acttctacta
     4741 gttctggttt caaacgaggt ggtaaaacag caagccccat tacttcgatc aacccaatgt
     4801 tctccttttt gatatgttgg acatctttat gtggatgaaa aatcccatca ggatgttctt
     4861 ctgaaacatt gttgtctcga aggacaaggt ccaactcaaa gtaattgtcc cttcttcgtg
     4921 caattggcgt aatcgtatgg tgtggtgtac catctgctga aaaagctctg atagatactg
     4981 tttcatccga atatgctttc catttttcca atactgagtt agctgcttca ataagcaagt
     5041 ttttctctgt tgattgcaaa cggatcacag acatcggcca tttcacgact cctgcgatga
     5101 cttgcggata atcttctagt ttgataagtt tttctatcgg tgcttttgcc ataggaaatt
     5161 catgacgtcc tgcctgataa tgatcatgag aaagaatcga tcctccaaca attggtaagt
     5221 cagcattcga accaacaaag taatgaggca agacttcagt aatccgaagc agtctagcaa
     5281 atgtttcctt tgtgattttc atcgggcggt gttgttctga taaaatgatc gaatgttcat
     5341 tgtaataagc ataaggggaa tattggaatc cccagctttc accgtctaga ttcattcgaa
     5401 taatccggtg attggtgcgg gctggatagt tcaagcgacc tctatatcct tcgttttcca
     5461 tgcaaagcat gcaggtagga tagtctactt ttaccgcctc gcgttctgct gcgatttgtt
     5521 ttggatcttt ttctggctta gataaattga ttgtgatttc cagttcacca tactccgact
     5581 ctgccggaaa tttgatgttt tttgcgatgg ctcttgtttt gatgtagtca tttttcttac
     5641 ttaaattaaa gaaatactca gttgcttcct ctgggctttt cgaatagtgc tgtgcaaaga
     5701 atgcattgac gactgaaggt ggcggtgtga gaaaatccat taattgcgct tcaaaaatct
     5761 cttgttctgc tggcaattct gaaagaacac cattttctct tgccttagcg acaagctgat
     5821 ccaataagtc tacagattct tccttaactg gtcttacttc aatgctttct agagcatctt
     5881 ctcctaccat ccctaaaaga cgattctgta aatataagcg gtccatttcc atccatccgc
     5941 cagcttcgat cgcaagtgtg gcaaaatcac aaatcgtttg actgattgac atatctcatt
     6001 cccctattct tcataaccat ttggatggct gacatgccaa tcccatgctg ttttgataat
     6061 cgctttgata tctgtgtatt caggtttcca gcccagaatc tctctcgctt tttcacttga
     6121 agcgactaat cggctaggat ctccagctct tcttggtgct atttctgctg gaatttcttt
     6181 tcctgtcact tctctagctg cttctagcat ttccttgact gagtatcctt tgctgctgcc
     6241 taaattgaag aagttgcttt catttccttc tttcaagtat tccaaagcta aaatatgtgc
     6301 agcaatcaaa tcttctactt gaacataatc tctgatacat gttccatctg gtgtatcata
     6361 atcgtctcca taaacagcca aggcttttct ttgaccaaga gctacttgta aaatgattgg
     6421 gacaagatgc gtttctggtg tatgatcttc tccgatagat gcatctgctt ttgctccggc
     6481 tacattgaag tagcgcaaag cgacatagcg catgccatat gcttggtcac accacttcat
     6541 cattttttcc atcatcaatt tactttcacc atacggattt ttaggattag ctggtgtatc
     6601 ttctgtgatt ggagactctt tcggctctcc ataagttgct gcagtagaag agaatacgat
     6661 tttattcacg ttgaactcgt gcatgacttc aagtaagatt tgcataccgt atacgttgtt
     6721 gttgaaatac atcaatggtt tttcgacaga ttctcctact aaagagcttg cagcaaaatg
     6781 gattactcct tcaatcggtt ccttttcaaa tacggaacgt agaaattctt tatcccggat
     6841 gtctccttca taaaaatgag catctggatg tactgcttgt ttgtgacctg tgagcaaatt
     6901 atctaccaca gctacttgat accccttttg aactagctgg tctacagcgt gcgaaccgat
     6961 ataaccggct ccgcctagta ctaaaattgt cataaaaaac actcctttga ttagtaaaat
     7021 tgtgtttgtt gattctatat aatttaaaaa acgaagttta cttttatggt gaaaacaaac
     7081 aaagtaaa
//
LOCUS       DAHUVV010000118         6911 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.121, whole genome shotgun sequence.
ACCESSION   DAHUVV010000118 DAHUVV010000000
VERSION     DAHUVV010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.121"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..182)
                     /locus_tag="NK767_002530"
     CDS             complement(<1..182)
                     /locus_tag="NK767_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCH6695479.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="NK767_002531"
     CDS             517..951
                     /locus_tag="NK767_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCH6695480.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="NK767_002532"
     CDS             974..1294
                     /locus_tag="NK767_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695481.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="NK767_002533"
     CDS             1435..1626
                     /locus_tag="NK767_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695482.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="NK767_002534"
     CDS             complement(1697..1954)
                     /locus_tag="NK767_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HCH6695483.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="NK767_002535"
     CDS             2112..2375
                     /locus_tag="NK767_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695484.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="NK767_002536"
     CDS             2399..2587
                     /locus_tag="NK767_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695485.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="NK767_002537"
     CDS             complement(2670..2855)
                     /locus_tag="NK767_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695486.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="NK767_002538"
     CDS             3070..3441
                     /locus_tag="NK767_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCH6695487.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="NK767_002539"
     tRNA            complement(3591..3663)
                     /locus_tag="NK767_002539"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="NK767_002540"
     CDS             complement(3709..4137)
                     /locus_tag="NK767_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695488.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="NK767_002541"
     CDS             complement(4197..4955)
                     /locus_tag="NK767_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCH6695489.1"
                     /translation="MKKKTFKKLLFLGGTTALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="NK767_002542"
     CDS             5250..5447
                     /locus_tag="NK767_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695490.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="NK767_002543"
     CDS             5553..6035
                     /locus_tag="NK767_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695491.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="NK767_002544"
     CDS             complement(6093..6689)
                     /locus_tag="NK767_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCH6695492.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2342 a         1061 c         1357 g         2151 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagt tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt atttgaaaaa
     6901 aacagctgat a
//
LOCUS       DAHUVV010000119         6798 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.122, whole genome shotgun sequence.
ACCESSION   DAHUVV010000119 DAHUVV010000000
VERSION     DAHUVV010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.122"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            68..3295
                     /gene="fss3"
                     /locus_tag="NK767_002545"
     CDS             68..3295
                     /gene="fss3"
                     /locus_tag="NK767_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCH6695493.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3408..3722
                     /locus_tag="NK767_002546"
     CDS             3408..3722
                     /locus_tag="NK767_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCH6695494.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3735..4109
                     /locus_tag="NK767_002547"
     CDS             3735..4109
                     /locus_tag="NK767_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCH6695495.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4119..4454
                     /locus_tag="NK767_002548"
     CDS             4119..4454
                     /locus_tag="NK767_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HCH6695496.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4537..5877
                     /locus_tag="NK767_002549"
     CDS             4537..5877
                     /locus_tag="NK767_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCH6695497.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            5955..6629
                     /locus_tag="NK767_002550"
     CDS             5955..6629
                     /locus_tag="NK767_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCH6695498.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6622..6786
                     /locus_tag="NK767_002551"
     CDS             6622..6786
                     /locus_tag="NK767_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695499.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
BASE COUNT         2303 a         1129 c         1503 g         1863 t
ORIGIN      
        1 tttataaaca aagatgttca agcgagcatc ttattttttt gtgcttaatt tgaaaggaga
       61 acatttcatg tataaaaaac aaaaaaagaa attatgtgta ttaactattt tgagtttggc
      121 tgttcaagca attgttggag caattgctcc tacgattact tttgctgatg aaattaccca
      181 tccacaaacg gtaaccgtag aactagattt agcacatcaa tatgctgttg aaggaacttt
      241 tagtgatggt cgtccaatgt cagaagtcac tgttccgcat tatgcagtgt acaacggctt
      301 taaacaagat attttctgta ttgaaccagg agtcccgatt tacaatgagt tcactcctgg
      361 ctatgagaaa aacccgttac cagatatgtc cgataaagcc aaattagttt ccgttttatg
      421 gaaaaaagta ggtacagatg cagatactca tattgtggca cagaagatga tctggcaaga
      481 agttaatggg tatacccttc actcaataaa acgatcagat ggtagtgccg taaatatcgc
      541 agccattgag gcaaaaataa atcaagccat tgacgattat cagaagaaac caaccttcca
      601 taatagtacg gctaaaacag tattagggca atcgactact gtgacggata cgaataatct
      661 gaatttgtca gagttcgatg aggttgtgga aaatgccgca aacattgatt atcgtgtgaa
      721 tggtaatcag ttgataatca ccccaaatgc caattcaaaa gaaagtggtg tgctgacgct
      781 taagaaatct gctggtaccg gaacacctgt cgcctataaa aaggctggcc aacagacttt
      841 aatggccggg gcgattgata aacctaatac ctacacaatc aaaattgatg tggaaacgga
      901 gggttcttta aagatcaaaa aagtcgataa agaatcgggt gatgttgtac caggaacggt
      961 tttccattta gactttggaa aaactttacc tgcaaaagac gtgactactg acaaagaagg
     1021 cattgctaca ttggatgaga ttcctcatgg tacaaaagta accattaccg aaaaatcagt
     1081 accggcgcct tatacgattg ataccacccc catgactacc accattaaag caggcgaaac
     1141 catttacgtg acttcaaaaa acgcacggga aaaagggcaa atcattctgg ataaaagcgg
     1201 agttgaaaca gggagtgatc tttggaatga caattattcc ttagctggta atacgtttgc
     1261 cattcgcaaa gacagtccaa ccggtgaaat cgttcaagaa atgaccacgg atgaaaacgg
     1321 tcatgcggaa acaccaaaag agattgctaa tgcgttggaa ttgggaacct attatgtgac
     1381 cgaaactaaa gctagtcatg ggttcgtgaa tacgttcaaa cctgtaaaag tcgaattgaa
     1441 gtatgccaat caaaccgtag cccctgttac cagtaacgta aaagggcaaa atcaagaagt
     1501 cactggcgaa accactttga cgaaagaaga caaagatact ggggataaag cacaaggtaa
     1561 ggcagtcttt gagggtgctg aatacacctt gtttactgct aaggatggaa aagctgttaa
     1621 atggagtgag gcttttaaac cagagttagc gaagggaacg aaaggttctg atgaaacggt
     1681 cactttgact ttagacgaaa agaaccaagt tgccgttaaa catttagcta tcaacgagta
     1741 ttattgccaa gaaacaaaag cgcccgaagg atatagcctt gatgaaacaa agtatcctgt
     1801 atccatcaaa aaggtggatg atagcgaaaa aaatgccgtg attacccgaa atgttacggc
     1861 aaaagaacaa gtgattcgct ttggctttga tttctttaaa tttgctggat cagctgcggg
     1921 cactaccgaa actggcttca atgatctgac ctttaaagtg tcgccattgg aagggaccaa
     1981 tgaaatcaca ggtgccgaag atgaagcgac cacagcttat aacgagcaat tagggtttga
     2041 tggttatggc aagtttgaaa atcttcctta tggggattat ttacttgaag aagtagaagc
     2101 cccagaaggt tttcaaaaga ttacgccatt agaaatccgt tctactttta aggaaaacaa
     2161 agaagacttt gtgaagagtg agtatgtctt taccattacc gaacaagacc aaaaacaacc
     2221 aattaaaacg gtgaccgttc cttacgagaa gctgacgaac aaagcatttt ctgttagttt
     2281 gaaccgttta atgctctatg atttgcctga ggaagaagat agtttaactt ctcttgcgac
     2341 ttggaaagac ggaaacaaag aattaacttc tctggattct accgagctag ttgataaatt
     2401 gagttacaat ctacatgaaa tcaaagaaga ctggtatgtg gtagcgcaag ccattgatgt
     2461 ggatgcgaca aaagctgccc aagaaaaaga tgaaaaagcc aaaccagtgg tgattgcaga
     2521 aacaagcgca acgttggcga acaaagagaa aacaggaact tggaaaattc agcataaatt
     2581 aaccgccgaa caagttttaa ataaaactat tgtcttattc aactatgtgt atgaaaataa
     2641 ggaagctttt gaagcaggcg ataagccagt agcgaaggat gttagcttga ataaccaagc
     2701 ccaaactgtc agttgtacag tggaacacca tgtttctatc caaacaaagg cccacctaga
     2761 aaacggttct cagaccttta ctcatggtga tgtggtggat atgtttgatg atgtgtcgat
     2821 tactcatgat gtactggatg gctcaaaaga agctttcgaa acaattctgt atgctttatt
     2881 accggatggt acaaacaaag aaatttggaa atctggcaag attgattatg aagtaaatga
     2941 taaagaattc accaaaactg tgcttgcgaa aaaagtagat accggaaaat atccagaagg
     3001 aaccaagttt acttttgcgg aaatcaatta cgataaagat gggaatatca atggaaaaca
     3061 caatgaagat ttgaaagaaa aatctcaaac cttaacacca aaagaagttc caaccatact
     3121 aagtacgccg aaacaaccgg aaacaccaac tgttccaagt ggctctcaag aatctagtcc
     3181 cacagtgaag accttccctc aaaccgggga gaaaaattcc aatgttttac tgttcattgg
     3241 ttttactttg atttttgcga cggcgggcta ttatttctgg aatcgccgga actaaggtga
     3301 tgagatgata caagtaaatc ctaagaacca acgtgcgcga ccgcctccta aaaaagtagt
     3361 cgcgctattt ttgtatccaa aactaacaat gaaaagagga aaagaagatg gaattgaaat
     3421 ttgtcgtgcc agatatggct gaaacctttg gaaagttaag ttatgccggt gaaggagaaa
     3481 ttataacaga aggatatgga cggaatacta cggtgattgg tcgcagttat catttgtatt
     3541 ccagtaagca acgggccgat gatattgagg tggtagtagc tgcggaagcc ggtgaaaagg
     3601 attttgatca agaccaaccg ctaaaagccg tgaatcccca tttggttgcc aaaggctatg
     3661 agattgagaa tcgtgggttt actgattacg tgttgtatgt tgatgattta gtgaaagcat
     3721 aggaggaaaa gaagatgaga ttaacagaag gtattgtcgt agattcaggt ttaacatttg
     3781 ggaagttgcg tttttccgca ttacgccgag aagtacggaa gcaaaatgag gatggaacaa
     3841 ttggtaatga ggtaaaagaa cgaacctata atttaaaatc ctctgcacaa gggcgaatga
     3901 ttcaagttag tatcccagct aatgtaccct tacgcgagtt tgcttatgat gcagaggtag
     3961 agttggtcaa tccaatcgtg gataccgttg ccaattatgt ttttcgagaa ggaactaccg
     4021 ttaattggtt cattaaggcg gatgatttag tcttgaagcg gcagccgaat caaggaaatc
     4081 ctacgaacca aaatgaaaga aagaaatagg tgaagattat ggaaattaat gttgaacata
     4141 ttttagattg tctcgatcaa tatggaaaag gcgagctgac tgaggaacaa ctcgttaaag
     4201 cattaacctc tgatgaaaaa atgtttctga ttatacatca aggtttactt ggagaaaata
     4261 gcgacagtga agaagactct gatgtactag actggttagg agaagaagag tctttcttta
     4321 tagttgtcga agtaaatgaa aacttatgtc ggcaggcaga atccattttg gaagaaattg
     4381 gtgtggagat gccggatgct attgaaggct ttttaaatca attggttgaa acgaaacaac
     4441 tcccctcctc gtagcagtaa atgactagca taactttttg aaattgccta atgaaatcct
     4501 attgggcttt ttcgattgga ggtggaaacc atcaaaatgt acaaaggaca tcgcatacgc
     4561 gcaggagatc agcacttggt ttatcacttt gttcttggct ggctactcgc tctgttcatc
     4621 ggttggatga gcgtctttta ttttcaagag tttagacaat ttgatatttc taagctatca
     4681 ctttctacta ttgaaatcgt ttggtctata aaagatttag tatgtctgct tggaagtctc
     4741 gctttttcag gagctatgat attgctttat attcactttt ttcttgatca ttggcgaagc
     4801 ttgtggcatc gacaaaagct agctcggatg attctagaga accattggta tgaagtgaaa
     4861 caaactcaaa gtgaaggctt tttcaaagat ttgaatagta gccgaaccag agagactatc
     4921 agttacttcc ccaaaatctg ttatcgaatg aaagatggat tactgtctat tcgcgttcaa
     4981 atttcacttg ggaaatacca agatcagctt ttaaaattgg aaaagaagtt agaaagcgga
     5041 ctgtattgtg agttagtaga aaaggaactc aaagactcct atgtggagta tactttactg
     5101 tatgatatga tcgccaatcg aattgggata gacgaagtag tggcagaaaa tgggactctg
     5161 cgattgatga aaaatcaagt atgggcttat gattctttac cccatatgtt aatcgctggt
     5221 ggcacgggtg gagggaagac ctatttcctc ctcaccatta tcgaagcact attgaagtca
     5281 gatgcagaat tgtttatcct cgatcccaaa aatgcagatt tagctgattt aggtacggtg
     5341 atgcctcatg tttattcaca aaaggaagaa atttctgctt gtgtggaaga tttctatgaa
     5401 cgtatgatgg ctcgtagcaa ggcaatgaaa gaaatgtcta actacaaaac aggagagaat
     5461 tatgcctatc ttggacttcc acccaacttt ttaatctttg atgaatacgt ggcttatatg
     5521 gagatgttaa cgacgaaaga aagtgcggtg attttgaata agctcaaaca aatcgtgatg
     5581 ttgggtcgtc aatctggttt ctttctgatt ttggcttgcc aaaggccgga tgcgaaatat
     5641 ttaggagacg gaatccgcga tcaattcaac tttcgcgtgg ctttgggtcg tatgagtgaa
     5701 ctcggatatt ccatgatgtt tggggaggtc gaaaaaaact tctttatgaa gcacatcaaa
     5761 ggtcgaggtt atgtggatac aggtggcagt gtgattagtg agttttatac gccacttgta
     5821 ccaaaagggt atgatttctt agattcaatt agtaaattag aaaagatgaa tgattgagtg
     5881 atcttcaata ctgactttat acttcaagta acgtataata cttttatata gtaggaagaa
     5941 aggggataat taatatggaa agtaatgatt cttatttaga gtatgcaaaa gcaaataagg
     6001 ataagtttat ttcttatatt atcaaaggga aatcccccga agttggacga gacgcagttt
     6061 ttatggcagg gagtcctggg gctggaaaat cagaggttgc cattggatta gctgagaatt
     6121 atgataacca tgtgattatt gatgcggatt actttcgcac ccagtttcct gattataatg
     6181 gaaaaaactc tagtctcttt caaaaagcat cttcttggtt ggtagagcag gcatttagat
     6241 atgtcttaga tcatggctat tcttttattc tggatgcaac ttttgcaata ttaagtgctg
     6301 aaaaaaatat ctcgcgggcg ttgaaaaaac attttcgagt gacgattttc tatgtttatc
     6361 aagaccctaa aatcgcttgg aaatttacac aagaacgtga aattgcagag ggacgtcatg
     6421 tgccgaaaaa ggcatttatt aacgcttttt tcaaatcaag agagaacatt gcaaaagtaa
     6481 agagtaaaca tccagatgta ttgcttcata tcataataaa agattatacg aataatattt
     6541 ccgaagttca ttatgctacg gataatctag aattattttt accactaggg tatagtagga
     6601 aagatttgga ggttcaatta catgactgag aaagaacaag taacgaaaat tgtaaagaaa
     6661 tacaataaga gcattgcgga tttatcggaa aatgctactg caaaagagtt caaaacggtg
     6721 attaaatatg tagccgatca ggccaatgaa aaacaacgga aattagttgg attaaataaa
     6781 aaatagtagg acaaatta
//
LOCUS       DAHUVV010000120         6605 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.123, whole genome shotgun sequence.
ACCESSION   DAHUVV010000120 DAHUVV010000000
VERSION     DAHUVV010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.123"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..1338
                     /locus_tag="NK767_002552"
     CDS             <1..1338
                     /locus_tag="NK767_002552"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCH6695500.1"
                     /translation="ILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFN
                     GLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASE
                     AGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNV
                     KYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPY
                     SANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRG
                     AAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFV
                     KGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEE
                     PIDMVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1328..2545
                     /locus_tag="NK767_002553"
     CDS             1328..2545
                     /locus_tag="NK767_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCH6695501.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2558..5707
                     /locus_tag="NK767_002554"
     CDS             2558..5707
                     /locus_tag="NK767_002554"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HCH6695502.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         2303 a         1085 c         1346 g         1871 t
ORIGIN      
        1 attttgggat atgcaattgc accagagtat cttttcaacg tcttagcgga tcaagcaaaa
       61 caagcaacgt tccaattgaa cgatctaaac aaagcctttg tgcaattggc atctacctac
      121 aatcaattta atgggttatt tgatgatgtg gatctccaat caaaaaaact aggaacagat
      181 gagcaacaac gaaatgtgac gattaccgaa gtcatcaaga aattgaatga tgtagatgtt
      241 ttagaacacg atggggacgt gatcggggat gcgtatgagt ttctgatcag tcaatttgct
      301 tcagaagcag ggaagaaagc cggagaattt tacacacctc atatggtttc tgatatgatg
      361 gcgcaaatcg taacgctcga tcaaaaggaa cgacgattct ttagtgtttt tgatccaacg
      421 atgggttcag gttcattgat gttaaacgta cgtaactact taacacaccc agataacgtt
      481 aaatatcatg ggcaagaact aaatacaacg acatacaatc tagcaaaaat gaacttgatt
      541 ttacatggtg tcgacgccga agaaatgaat ctccgcaatg gggatacgtt gaataaggac
      601 tggccaacag atgaacctta cacatttgat gcagtagtaa tgaacccacc ttattcggct
      661 aattggtcag cagatacgac tttcttagat gattctcggt ttaatcgtta tgggaaatta
      721 gcgccaaaat ccaaagcaga ttttgcgttt cttcttcatg ggttctatca tttgaaggaa
      781 acaggtacca tggccatcgt cttaccacac ggagttttat tccgtggcgc tgcagaaggg
      841 gtcatccgtc aaaaattact agaagatggc agtatttatg ctgtgatcgg tatgcctgcg
      901 aacttgttct ttgggacatc gattccaacg acggtgattg tgttgaagaa gaatcgtcaa
      961 actcgagatg tattattcat tgatgctagt cgtgaatttg ttaaagggaa aaatcaaaat
     1021 aaactatcgg aagaaaacat tcaaaaaatc ctcgaaacct acgctgaaag aaaagatgtc
     1081 gagaaatacg ctcatttggc aacctttgat gaaatcaaag aaaatgacta taacttaaat
     1141 atcccacgct atgtagatac tttcgaagaa gaagaaccaa ttgatatggt tcatgtcgga
     1201 aatgacatca agaagatacg acaagaacag caagtgctag aaaaaaaact tttagaagct
     1261 atctcttccc tgcaaacaac acctgaaaat gaagcgtggt tacaaggtgc gctggaggtg
     1321 ttcaaacatg agcaatgata cacaaccaga aatccgtttc ccaggattta cagatgattg
     1381 ggaacagagg aagttggggg acttcgttgt agattatgtt gaaaagacaa gtgtacaaaa
     1441 tcaatttcct atgctaactt catctcaaca aaaagggata gttttgcaag aagactattt
     1501 tgcaaatcgg caagtaacta cagaaaataa tattggttac tttgtactac ctagaggata
     1561 tttcacgttc cgtagtcgaa gtgataatga tgtgtttgtg tttaaccgta atgacattat
     1621 tgatagaggg attataagct atttttatcc tgtatttact ttaaaatctg ctgattccga
     1681 cttcttctta agacgtatta acaatggaat ccaacgacaa ttatctattc aagcagaggg
     1741 aacaggtcaa catgttctgt ctctaaaaaa gtttaaaaat atagtagcaa tgtttcctag
     1801 tgaggaagaa caacaaaaaa tcggcacctt cttcaaacaa cttgacgaca cgatcgctct
     1861 tcatcagcgt aagttagatt tgctgaagga aacgaaaaaa ggattcttac aaaaaatgtt
     1921 ccctgaaaac ggagcaaaag tgccagaaat ccggtttcct ggttttacgg aagattggga
     1981 agagcgtaag ttgggagaat taggtaaaac tcaatcggga attggatttc ctgatgccga
     2041 gcaaggtggt tctgaaggta ctccattttt taaagtttcc gacatgaata acattggtaa
     2101 tgagtatgaa atgagaaatg caaatcatta tgtatctaat gagcaaattg agcgtaaaaa
     2161 atggaagcca ataaaagatg tgccagcgat tatatttgct aaagttggcg cagcaataat
     2221 gcttaataga aaacgtttgg tcacaagccc tttcttaata gataacaata ccatggctta
     2281 tttatttaat aatacttggg atatctattt tggtaaaata ttgtttgaaa ctataaattt
     2341 gccaagatac tcccaagtag gggcattgcc ttcttataac agttctgata ttgagaatat
     2401 cagtgttaag gttcctgtaa aggatgaaca acaaaaaatc ggtactttct tcaaacagct
     2461 agatgatacg atcgctcttc atcaacgtaa gttagattta ctaaaagaaa cgaaaaaaag
     2521 cttcttacaa aagatgtttg tctaggaggg aagaagaatg aaaattagtc accgtgagga
     2581 agcagaagtc gaagaacagt tgattcgtgt acttggagaa gggcataatc agtggacgta
     2641 tcgtccggat ctaaaatcag aagaagacct ttgggtcaat ttacgccaaa aaattatctc
     2701 aaataatcaa gcagaactga atgattttcc tttgaccgat aaagaatttg aaacgatcaa
     2761 aacggaatta ctgctgcgaa cgaaaacccc atttgatgct gcgaaatggt taaaaggtga
     2821 aaatgggatg gctcggatca cgattgaacg ggaagattcg caattgggtt ctgtctcact
     2881 tatcctttat tccaatcaag acattggtgg aggaatctct acttatgaag tggtccatca
     2941 aattgcaaaa cgtggaagta acatagaagc acgggatcga cgttttgacg tcaccttatt
     3001 aatcaatgga ttaccgattg tccaaatcga gctgaaacaa gtaactgcaa aagatggtgt
     3061 ttatcaggca tttaaccaaa tcaaaaaata tgcagaagaa ggtatgtttc ggaacaatat
     3121 cttttcgacc ttacagttgt ttgtggtttc caatgaacaa accactcggt attttgcaaa
     3181 tgccttaccg aaagacttgc accctaagtt tttatttagt tggcgaacga aagacaatga
     3241 aaaagtagaa aacttatatg agttttgtaa acaagtgtta aatatcccgg atgctcatcg
     3301 tttgattgcg gattatacaa tcgtcagtga agatcaagac aataaaaccc tcatggtgtt
     3361 acatccttat caagtacatg cgattcaagc cttgtttatt gccgcaaata aacatcaatc
     3421 agggtatgtg tggcatgcga ctggttcagg aaagacgttg actagttttg tatctactaa
     3481 gcttttagca cgaaaatcgg ggatcgatcg aacgatcatg ttagtggacc gcaaagattt
     3541 agataatcaa accaccacag agtttacaaa atttgcatcg gaatttaata ctgggatttc
     3601 ttccgggaac gccaaagcga atagcttgat cgttggaacg ggaagtgcca aagaattaag
     3661 tgaaaccctg ttggcagatg ccaatgcaaa tgttgtaatc attacaaccc gtcaaaaact
     3721 agatgcggcg ttgaaatatg caaagaaaca agaagagaaa aaaggaacca atcgcttcca
     3781 aaaattaatg ggccaacata tcgtgtttgt agtagatgag tgtcatcgag cgttaagtgc
     3841 tgaaaatatg gaagaaatca aaaaaatgtt tccgaaatcc acttggtttg gctttacagg
     3901 tacaccgatt tttcctgaga atcggaaaca agccaaaggg caattggcac gtacaaccca
     3961 tgatcagtat ggagaggtct tgcataccta taccattaag aatgcgctag aagatggctc
     4021 tgtcttagga tttcaagtag agcatgaaaa caccgtggag ccgacttcac tagagaataa
     4081 aatctatcgg aaattaaaag aagtagaaac atatgcagag tattcatcag aacagatcaa
     4141 tcgtatgatt gatcaaatgg aacctgtcaa aaaagaaagt tatttggacc cagctgtttt
     4201 tgaagcagat gaacatatcc aaaaagtcat tcataaaatg ttccgtccag ataatgccta
     4261 tacaaaattt gattttcgaa atggcaggcc aacgaaatct gcaatcttaa ccacgagttc
     4321 cattgacatg gccaaaaagt attatcgggc catcaaagag atgacgaaag agcctgattg
     4381 gttaacaaaa gagtttccca atcagctcat tcgtgaagga cgaacgatgg aggatcctga
     4441 ttttccacga atcgctatta cctattcatt agaagaaaat agccgagatt caagtgccca
     4501 acaagaagaa atgcaaaaaa ttattgagga atataatcag tattatgata cttcctggtc
     4561 attagcagac attgagcgtt ataacggaga catcaataat cgtttggcac gaaaaagagc
     4621 ggaattcaaa caatttggga aacaaatcga tttagcgatt gtagtggatc gtctattaac
     4681 aggttttgac gcccctacga ttcaaaccct atttgtggat cgtaatctag agtatgcagg
     4741 attgatccaa gctttttcac gtacgaatcg tacgtatcct gataagacaa aaggattgat
     4801 tgtgacgttt cgtaagccag cgacaatgga gaagaatgtc gaggatgcga caaaactgta
     4861 ttcaaaagca aaagaagaat caggtttgat ttatccaagt tatcaggaat ccaaaaaaag
     4921 attcaaacaa gcatatggta agttaaaaga ggtttcatcc atggaatcca ttgatgaaca
     4981 tacgccactt gaaatccgta tcgagtacgt caaagcattt caagaattga atcgtgctta
     5041 tgaatcgtta gtgacttatg acgattacaa tgatgacatg gtgaaatctc caactttgaa
     5101 caatcaaatt caacttttgg aagaacaaat tggcgtgtac gagaccgtga agggttcatt
     5161 aatagaggaa gaaccagaaa aagaaggaga agatttttca acgatcgaat tttatagtga
     5221 aaataccact aagttgtatg atattgattc aacttatatt gatcaattac taggaactta
     5281 tgctgcgaat agttcagatg cacgagaaga aattgaaaaa gctttggcta aattgaataa
     5341 aacagaagga gtcaaagaag tttaccgaca aattttaaat gcatttgatg tggggacttt
     5401 agatagtaat gaagatatct ttgtcattaa acgtcgctat tttacacaag cgagtgatga
     5461 tttgattcat gtcttttcca atgagtggtt tgtttctgag aaagaattac atgcttctaa
     5521 tctgcaatat ctaccaggtg aagatccaat tcctaatatg aaagcaatta tcaacagtaa
     5581 ggattacgaa ggatataaag ccaaacatcc agaagcgaag ccatttaaat acccacaaga
     5641 aatgaagcga gcatggcgta aaatgttaga tgatgagttg attccacttg agaatgaatt
     5701 gaggtaaacg agaagagtta agaaaattgt acgattcaaa gatggtagtc aagtggaagt
     5761 gtagaaaata gggatagatg tcttttattt gttcacattt tataacgaag tatttaagac
     5821 agctattgaa taatgtttcc tccacctttt tcattcattt accccacccc gttgttatat
     5881 gaccgtgttt tgagaggaac caataaaaag cataggctaa ctaggggttc ctttttcttt
     5941 tgtttttttg gaatccgctt aagtgaaatg tatctgaaat gaaacggata taaagtgatt
     6001 ttttgctata atgacaaggt tttagctgtt tcagatacca tgtaacccaa aatagtttat
     6061 tgagaaaata ggagagtatt tgatactaac tagtaggaaa aaatgagata aaaattaaat
     6121 ggaatcattt caggtaatct caaataaaaa aatatacagt cctgtggcac tttacctatg
     6181 ggactgtata ttttttactc tcagcgggct tgttttgact ccgtgtctga ctccgtcgta
     6241 ttgattttaa atgatattcg atgaccattt aacaggtcat ttttgaaatt tttgtctcta
     6301 tatgactcag tttgataatt attgatagat caatttgggt ctatatcgaa caagggaata
     6361 atccaataaa aaatgattta gaatatcaaa ataacaaaaa agaggactac ctgtattcgt
     6421 cctctttttt gactagattt tgaccagtct aatttacatt ctaatatatt catatgagct
     6481 aattttttga ttaaatggta agtttttcct tgtttgtttt agtttaaact agtgtgtaat
     6541 ttaaaaatct atatggaagg gtctgtgggg taatacttaa ataaaggcta ggaacctgat
     6601 aacat
//
LOCUS       DAHUVV010000121         6422 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.124, whole genome shotgun sequence.
ACCESSION   DAHUVV010000121 DAHUVV010000000
VERSION     DAHUVV010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.124"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(4..249)
                     /locus_tag="NK767_002555"
     CDS             complement(4..249)
                     /locus_tag="NK767_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695503.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(266..1117)
                     /locus_tag="NK767_002556"
     CDS             complement(266..1117)
                     /locus_tag="NK767_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCH6695504.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1283..2287
                     /locus_tag="NK767_002557"
     CDS             1283..2287
                     /locus_tag="NK767_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HCH6695505.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(3070..3627)
                     /locus_tag="NK767_002558"
     CDS             complement(3070..3627)
                     /locus_tag="NK767_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695506.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(3806..4417)
                     /locus_tag="NK767_002559"
     CDS             complement(3806..4417)
                     /locus_tag="NK767_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695507.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(4530..4787)
                     /locus_tag="NK767_002560"
     CDS             complement(4530..4787)
                     /locus_tag="NK767_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695508.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(5384..5662)
                     /locus_tag="NK767_002561"
     CDS             complement(5384..5662)
                     /locus_tag="NK767_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695509.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(5696..5947)
                     /locus_tag="NK767_002562"
     CDS             complement(5696..5947)
                     /locus_tag="NK767_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695510.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1991 a         1231 c         1021 g         2179 t
ORIGIN      
        1 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
       61 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
      121 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
      181 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
      241 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
      301 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
      361 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
      421 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
      481 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
      541 ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa
      601 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
      661 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
      721 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
      781 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
      841 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
      901 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt
      961 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
     1021 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
     1081 catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
     1141 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
     1201 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
     1261 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
     1321 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
     1381 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
     1441 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
     1501 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
     1561 acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt
     1621 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
     1681 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
     1741 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
     1801 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
     1861 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
     1921 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
     1981 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
     2041 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
     2101 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
     2161 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
     2221 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
     2281 tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt
     2341 gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa
     2401 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag caactccttc
     2461 ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa atggtgtcca
     2521 aaagcttgca tctgccagat tatctcattt cttatgacaa gctaagcaaa ttatctccac
     2581 caaataattt attcatattt ttgacttgcg catactaact aattcttaaa ttttcataca
     2641 aaatttctca atttccctta acgagtaata tttatgtacg gtgattctca ctagtatccc
     2701 aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc ttttatgtat
     2761 gctgttagtt gattagatgg tcatttgttg agctaggtcg tttattttaa acaagttgtt
     2821 tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa atgcatacgc
     2881 atctgtattt gccactttga ttcgtataaa gctaacaact attaactcct ctcagctaat
     2941 agttgctctc ttttttattc agattcattt ttgctgatca cagcctagta tttcaatggt
     3001 tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa atggattagt
     3061 agatttcgat tattcaaagc gattttctat tggtggatcg tgttcccaac taataagacg
     3121 gattccaaac ctatcgtaca gatcttctaa accacttata aattttgcac ctatttcagg
     3181 tgttttattg cgtggatatt ctattgttac gccattaaat ggataccaat taggattagg
     3241 ctctcttttt ttctgtacat ctcccataga atagttagca ctttctggga cataatatat
     3301 tcttcgacca tcctctaatt catctaataa gatatttcgt gatcgtctat cacttgctaa
     3361 atgtagacgc cacgtgcgat accatgccat gctacctgca tctgcagaaa atccatcaaa
     3421 taatgcaaca attgtatctt ctatcacaat cctttcccca tctacttcat attctaatct
     3481 aaaatcaaac tcccctcggg taatttctgg tcttggtggc ttaggaccca atattattct
     3541 acttaaaacc gcattcgttc ccagatgaaa cacagctata acaacaataa tgattaatat
     3601 tttgtttcta tttgataaat ttctcattat tcgcacatca cctctacatt ctcacatatt
     3661 agttcgtaat actcctttaa tatcaaaata tctactggtc gtaataatat tcgctatgaa
     3721 ttcctactac tcgtataaga aaggccaaat catcagcttt tacttgctga tgatttggct
     3781 tgtttctatt tatcgtaatt gttttctaat caccaaaatt gttaacaatc ggttcagata
     3841 tttcccagtt gattaaggta attccgtatt gtgtcaaaac ttcttgcgct tctccgaaat
     3901 taagtacagt tcttatgtat ccactttctc ctaatataaa gtctacctct cttttaaaac
     3961 cttcctcgag atttctaagg gctatatgtg gttttaatga cattgattgt acattttccc
     4021 ccatataata ttgcgcactt cctcgactaa aatagattcc tagatcgtcc ccaaaaggta
     4081 gatcttctct accactagct aatctacttc tccatcttcg ttctcttttc atagaacctg
     4141 cgctgaactc acacccgtta aactcagcta tgacagtatc ttcaattatg ttaatctgtc
     4201 catctagttc gtattcaatc ctaaatggaa attctccata ggtgatttcc ggttcgggta
     4261 ttttaggtga gattaatgca aagaaaggaa atattaagct tggcaacatc aaaaaaactc
     4321 ctcctatgat cacaattccc gatgtaatcc aaaatataat ttgtacaatt ctcattgatc
     4381 cattgtattc cattttattc cccctattac tattcatatc cgcctatcta tatcgatctt
     4441 ccaatgcaga gataattgct tccatacctt gattttttac tgagttacct atgcgagata
     4501 tcattctcca ctaaggtgca tcatattgtc tatctaacat gattaattct ccaataggtg
     4561 tatactcctc caaaaacaac atttaatgca tctccctcga caacataagc taccatgttt
     4621 gctgtatatg tgtttgagtc taattcataa gcagataaat ttactgtcgc tggattaaat
     4681 ataatcgcat tcgtatttat agctactgaa tttgctgctt cagcaccacc ttttgaatgt
     4741 ccgatcattg tgatttcaat agtaggattt acgtggacag ccctcaccgt ttaccctgac
     4801 cattatagaa atcgcttact tttttgaact acgcaacaaa ggatacaatg ctcctaactt
     4861 cactgtctcc atttttgtcc actaaggaat ataaaaaata ggcattaatt catttaattc
     4921 ggtcacaaca cgttaccttt taaatcatta tactgtatct catacattat tatcaataaa
     4981 ctattataaa aatgcaaaaa atgctttatc aatctatttt gtaattggtc ataatatcat
     5041 tgaaatcata ccctgtatcg atttttccta tgaatctcgt ctatttgtga ttgacgaaaa
     5101 agaggcaaga cagacactgg aatggtaatt ttacccacta ttttgctcca actatttgta
     5161 atcgtaccca cttttgaaat accgagatga tcattttaat tgatatgctg attcccaagc
     5221 aaaaccccaa gaaattaaat gctctatcgc atctagcttc ttgggttttg cttatataaa
     5281 ttccaacatt taaatttttt atgctgttcg gtctcagaaa tgccgttttt gcttatatca
     5341 caatatcaag atgtcaaatt attcgtgttg ccgatgactt tgattaattt ttttcaaatt
     5401 gtttattaag actaatgctg tctttttgca gataccaatg ttcgaacttt ttaacattat
     5461 catatgatcc tgcaagtttt aacttcttac cattagatgt tatcaaagtt ataattaacc
     5521 ttgccttttt cccattagga tcttttattt tatgaatttt ctgcaggtaa tctctcctaa
     5581 tggcagctat ttttacctca tcagtgctga taaaccattg atcatccaca tacatactgc
     5641 ctgttatgtt gtgtttttcc atttcttccg agaacgaaaa gtttaaggct cgttcttact
     5701 tccaaagttg aatgagtcca tggattggac ttagtgtgag aattgagaac aacaataaga
     5761 atccatatcc tcccgcaaac atccctaagg ctatactgtc tctccatcca taagtaggat
     5821 tagaagagaa aagagtcacc gtttgtaata atatgaaaag gaataatcca gcacaaacac
     5881 caaagataac aactccaaac attgaatttt tatacaatga ccatttattt ataggctttt
     5941 ctttcatttt acttccccct actgttctgt tcacacggaa tgaattcaaa gttcataagc
     6001 cctcctcttt gaacatatga atacgacttg ctaactatac tttaaaaaag cttgttttaa
     6061 gcggttatag gcgagttgta atctctattc atctcattag caaataactt gccgaaaagt
     6121 ctactgaaat taaagcagat agtggaagaa ttatttagcc aggatagaca ctatttgtga
     6181 aacacatgtc tttacttttg aaattctatg atgaaaagcc ttggaataca gtagctctta
     6241 attggtaata ctttttccat tataaataac ctcttttcat tttatattta tcccctctaa
     6301 aaataatatc taatctaata ttttaaactt atatttccct cgctgtgcta atagagcatt
     6361 actaaataaa tagaaactag aaaaaagcat tacgcctgcc agaataaacc aattatttgt
     6421 at
//
LOCUS       DAHUVV010000122         6280 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.125, whole genome shotgun sequence.
ACCESSION   DAHUVV010000122 DAHUVV010000000
VERSION     DAHUVV010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.125"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(74..262)
                     /locus_tag="NK767_002563"
     CDS             complement(74..262)
                     /locus_tag="NK767_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCH6695511.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            532..894
                     /locus_tag="NK767_002564"
     CDS             532..894
                     /locus_tag="NK767_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCH6695512.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            946..2628
                     /locus_tag="NK767_002565"
     CDS             946..2628
                     /locus_tag="NK767_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HCH6695513.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2745..4781
                     /gene="recG"
                     /locus_tag="NK767_002566"
     CDS             2745..4781
                     /gene="recG"
                     /locus_tag="NK767_002566"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCH6695514.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4830..5831
                     /gene="plsX"
                     /locus_tag="NK767_002567"
     CDS             4830..5831
                     /gene="plsX"
                     /locus_tag="NK767_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCH6695515.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5953..6195
                     /gene="acpP"
                     /locus_tag="NK767_002568"
     CDS             5953..6195
                     /gene="acpP"
                     /locus_tag="NK767_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCH6695516.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2091 a         1094 c         1427 g         1668 t
ORIGIN      
        1 aaaagtccca cccttaaaaa tttttattaa ataaaaaacc gttcactatc tgaacggtcc
       61 aagtatcatt caattaaacg cgttcaactt ttcctgattt caaagcacga gttgacaccc
      121 aaactttttt aggttttccg tcgaccataa cgcgaacttt ttgtaagtta ggtttaacag
      181 tacgttttgt tgagttcata gcgtgtgaac ggttgtttcc actttttgtc ttgcgacctg
      241 taaagtaaca aacttttgcc atggtaattt cctcctttgc cttgatttgg agcaatagaa
      301 taattcagct cataccagac taacatatca tatagaatgg gaatttgcaa gtacattttc
      361 atagatacaa ttgacaattg agaagaataa ttgtaaatta aacaaggtta tacctgaaat
      421 tttgcaaggt ttttgagtac tttgggtaac tgccttttat ttcaagttgc gctatgataa
      481 aatatgatag aagaaaaatg ggagtttttt acccgttaag gaggctcatt catggctgta
      541 aaaattcaaa cgtcagctgg aaccattgaa attaccaatg atgtaattgc aacagttgtt
      601 ggtggcgccg cgacagatgt gttcggtgtt gtaggaatgg ccagcaaaaa tcaaattaaa
      661 gataatatta ctgagatttt gcgtaaagaa aattactcaa gaggagtagt tgtccgccaa
      721 gaagataatg gtattgctgt agatgtttat acgattatca gctatggtac gaaaatctct
      781 gaagtttccc gcaacgtcca agaaaaagtg aaatacaatc tagaggcatt attaggcgta
      841 actgccaatt cagttaatgt ctttgtacaa ggtgttcgtg tactgccaga ctaggcagga
      901 gactggctgg taactcagca gtcgatctga aggaggatta tctaggtgag tgtaacagaa
      961 ataagtgcaa gccagttcca ggaaatggtc caggcaggtg cgaatcgtct gcaagtaaat
     1021 gcagaatacg tcaattcatt aaatgtattc cctgtgccag atggcgatac gggaacaaat
     1081 atgaatctat ctatgactag tggagctaag gcagtagtag attcaacctc agaaaaagta
     1141 ggagaattag cagcaatgct ttcaaaagga ttgctgatgg gtgcgcgcgg aaattccggc
     1201 gttattttgt ctcaattgtt tcgtggattt tctaagcaaa ttccagatgt tgtctcatta
     1261 aatgcttctg atcttgctgc agccttcact catggagttg aaactgcgta taaagcagta
     1321 atgaaaccag tagaaggaac gattttgaca gtagctagag aagcagcgaa agctggagaa
     1381 aagaaagcaa aaagtactga tgatgtcgtt gaagtaatga cagctgttgt aaaaggagca
     1441 aagcgtgctt tagctaaaac tccagacttg cttccagtcc taaaagaagt cggtgtcgta
     1501 gacagtggag ggcaaggact gctgtttgtt tatgagggct ttttaagtgc attgaatgga
     1561 gaattccaag cggatgaaac atatgaacct tctccagcag aaatggatga tatggtcaat
     1621 gcagaacatc atcgcagtgt ccaaggtcaa atggcgacag aagatatcca ttttggttac
     1681 tgtacagaaa tcatggtaaa aatcggagaa ggacctacag tcgatagtac attcgattac
     1741 gaagaattcc gtaactattt agatggtata ggagattctt tacttgtggt caatgatgac
     1801 gagatcatca aagtccacgt gcatacagaa aacccaggag aagtcttgaa ttatggacaa
     1861 aaattcggtt cattagtcaa aatcaaagtc gacaacatgc gcttgcagca tgagacgatt
     1921 ttggaacacg atgaagaagt gaacgagttt gcgaaaaaag aaataccacg tatcccgtat
     1981 ggtatcgtag cgatcgctgc tggtaaaggc gtccaagagc tgtttgaaag tttaggcgct
     2041 aattatgtta tcagtggtgg acaaacaatg aatccaagta cggaagacat tgttaaagcg
     2101 attcaagaag taaacgcaga taaagtgatt gtcttgccaa acaataagaa catcttcatg
     2161 gcagctgatc aagcagcaga agtagcagat atcccagtag cagttgttcc ttcaagaacg
     2221 atttcccaag gattgacagc tatgctttca ttcaatgatc aagcttcttt agaagaaaac
     2281 aaagaaacga tgacagaaat gttgtctagc gttgtgagcg gtcaagtcac tcacgcgatc
     2341 agagatacct caattgacgg tgtagagatc aaagaaggag actttttggg catgatcgat
     2401 ggaaaaatcc tgatctctaa cccagatatc acagcaacag cgttaagtac actagaacaa
     2461 atgatcaatg aggacacaga gatcgtaacg atcttggcag gtgatgaagg aacagttgaa
     2521 caagcaaatg aatttgctga gaaactaaca gaaaaacatc ctgatttgga aatcgagatc
     2581 caccaaggag atcagcctgt ctatccttat ctattttctg ctgaataatt tctgtgtcag
     2641 aatatctttc taacacagta ttcattcata aaaggccttc ggttagtgat caggtatgat
     2701 cgctgaccaa gtcctttttt tgttaaagga gggaggaaaa atttatgcgt tctttagatg
     2761 atccaatcac tatgctggca ggagtcggag aaaaaagagc agctagtctt gccagcttgg
     2821 gcatccaaac gattgaagac ttactgactt attatccttt tcgctacgaa gatattcagg
     2881 aaagaaatct aaacgagatc caggatcaag aaaaggttac tttgaaagga atcgtagtct
     2941 cgcctcctgt gatgaatcga tttggttata aaaaaagccg attgcaattt cgtatgatgc
     3001 aagaacatga tgtgttcaac gtttcttttt ttaatcaacc ttatctaaaa gaaaaagtca
     3061 ttttatcgga ggaaatcgcc gtatacggta aatgggatgc aaagcgtaaa gccttgaacg
     3121 ggatgaagat tttaggttct caaacaatgg atgacttttc gccgatatat cacgtcaaca
     3181 aatcaattcg tcaaacgaca ttagttgatc taatcaaaaa agggtttcaa gaatacggca
     3241 cattgattga agaaaatctg ccaaacaacc ttgtggagaa atatcgactg cttgaccgag
     3301 cttctgctgt aagaagtatg cattttccaa aagaccacga agaaaaccat caagccaaac
     3361 gacgtgtcgt ttttgaagag ttctttttat ttcaaatgcg tcttcaagga ttgaaaaaag
     3421 cagaaaaagc agaaacgaat ggtttggaga tcctttatga tgtccaaaaa ttgaaacaat
     3481 ttactcaaaa gctacctttt gaactgacaa atgcacaaaa acgtgtaacg aatgaaatct
     3541 gtcgggatct tcgcagtccg cagcatatgc agcgactttt gcagggagat gtaggtagcg
     3601 gaaaaacagt ggttgccgcg attgcgttat acgctaccgt tacagcaggt ttccaaggtg
     3661 cgttgatggt gcctacagaa atcttggcac aacaacatat ggaaagcttg caacagctgt
     3721 ttgatcccaa tgaagtaacc atcgcattga ttacggggtc aacgaaagca aaagaacgac
     3781 gtgagctcct tgaaaaacta gagcaagggg aaattaacgt cgtgatcggc acacatgcat
     3841 tgatccaaga tggagtcgaa tttcagaatt taggtctagt tattacggat gagcagcacc
     3901 gttttggcgt gaaccagcgg aaagtacttc gtgaaaaagg ctggcgacca gatgtcttat
     3961 ttatgacagc gactccgatt cctagaacat tagctattac agcatatggt gagatggatg
     4021 tatctatcat tgatgaactt ccagctggac gtatcccgat cgaaacacgc tgggtaagaa
     4081 cacctcaatt ggacagtgta ctggattgga cgaaaaaaga actggcaaga ggacatcaaa
     4141 tgtatgtcat ctgtccgctg attgaagaat cagaagcatt agatgtaaaa aatgcagtgg
     4201 aaatctatga aaaacttcgt gaactatttg caccaaaata tgaagttggc ttgttacatg
     4261 gtaaaatgaa gaatcaggaa aaagaagtaa tcatggaagc cttcaaagac aaccagatgc
     4321 agatattagt ttcgactact gtaattgaag taggggtaaa cgtgccgaac gcgaccgtca
     4381 tgctgatcat ggatgcggat cgatttggat tggcacagct gcaccaactt cgtggacgtg
     4441 ttggacgcgg aagtgatgct tcctactgta tcttagttgc taatcctaaa aatgaactag
     4501 gtgtggaacg gatgaaaatc atgacagaga ccaccaatgg ttttgtacta agcgaaaaag
     4561 atctggaatt acgtggtcct ggtgaagtat ttggcttccg tcaatctggt ctgcctcaat
     4621 ttgcgattgc tgacttggtg acggatggaa atgttttgga ggtagccaga gaagaggctg
     4681 cagccctttg gcaaataaag gattggcaga tgcttcctga gtatcagcct ttagcgcaaa
     4741 gcctccaaat agaaaatcag gataatcgtt atttcgatta gcagactgtg ctatacttta
     4801 tactgatgac ggaaaaagga gtggaaacaa tgaaaattgc cgtagatgca atgggcggag
     4861 atcacgcacc tcaagcaatt gtagaaggcg tggctttagc gcagcaagat tttccagaag
     4921 tagaattctt actttacgga aaagaagcag aaatcaagaa atacttaaca aatgaaaaaa
     4981 acatcacgat tatccataca gacgaaaaaa tcaatagtga tgatgagcca gtaaaagcaa
     5041 ttcgacgaaa aaagactgca tcaatggtcc ttgctgcaca agcagtgaaa aacggtgaag
     5101 cagatgctgt attttcagca gggaatacag gcgcgctact agcagcaggg ttgttcatcg
     5161 taggacgtat caaaaatatc gaacgccctg gcttgatgtc taccttgcca gtagtaggga
     5221 aagaaggtgc cggtttcgat atgctggatc ttggcgctaa cgcagaaaac aaaccagaac
     5281 atttgttgca atacggtatt ttaggttctt tttatgcacg taaagtccga ggtatcggac
     5341 gtcctcgtgt cgcactctta aacaacggaa cagaagcaac aaaaggtagt gaagtgacga
     5401 aaaaagccta tgaactcctt cagaatgaaa caagcttgaa ttttattgga aacgtagaag
     5461 ctcgtgagct actcaatggt gtggctgatg tagtggtcac agatggattt acaggcaacg
     5521 ctgttttgaa atctattgaa ggaacagcaa tgaatatgat gaatctatta aaatctgcta
     5581 ttctaaatga agggatcaaa ggaaaaatgg gcgctatgct tttaaaagac ggcttacgtt
     5641 ctttgaaagc agaaatggat tattccaaac atggcggtgc cgttttgttt ggcttgaaag
     5701 caccagtcat taaaactcat ggagcaacag gccccgatgc tgttcgctat acgatccgcc
     5761 aaatccacac aatgcttgag acagatgtag ttggacaatt agtcgaacaa tttgaaaaaa
     5821 gcgaagaata gtatttggat atctgaagcg atccgttcca ttgaaaaaaa ggtggacatt
     5881 cctgaaaaat gccagtaaaa ttagccatgg tgtaggtaat tgacaagtgt atgattcttg
     5941 gaggtgaaaa agttgactcg cgaagaagta tttaacaaag tagcgaaaat tatttcaaat
     6001 cattttgaga tagataccga taaagtgacg gatgaattga acatcaaaga tgatttaaaa
     6061 gctgattcaa ttagtattat ggaattcgtt ttagagcttg aagatgaatt cggtacggaa
     6121 atttcagatg aagacgctga acaaattgaa acagtcggtg gcgcagtaga ttatatcagc
     6181 agccatctta aataatttca aataaaagca aagaaggata aaaagctgca tgcagctttt
     6241 tatccttctt tttttaatct ttttgacacg taaaagtcat
//
LOCUS       DAHUVV010000123         6188 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.126, whole genome shotgun sequence.
ACCESSION   DAHUVV010000123 DAHUVV010000000
VERSION     DAHUVV010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.126"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..404)
                     /locus_tag="NK767_002569"
     CDS             complement(<1..404)
                     /locus_tag="NK767_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6695517.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTT"
     gene            647..1102
                     /gene="tnpA"
                     /locus_tag="NK767_002570"
     CDS             647..1102
                     /gene="tnpA"
                     /locus_tag="NK767_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCH6695518.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1367..1465
                     /locus_tag="NK767_002571"
                     /pseudo
     CDS             1367..1465
                     /locus_tag="NK767_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2148..2966)
                     /locus_tag="NK767_002572"
     CDS             complement(2148..2966)
                     /locus_tag="NK767_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCH6695519.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3127..3816
                     /locus_tag="NK767_002573"
     CDS             3127..3816
                     /locus_tag="NK767_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCH6695520.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            3830..5332
                     /locus_tag="NK767_002574"
     CDS             3830..5332
                     /locus_tag="NK767_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCH6695521.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     GYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5345..5821
                     /locus_tag="NK767_002575"
     CDS             5345..5821
                     /locus_tag="NK767_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCH6695522.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(5918..6028)
                     /locus_tag="NK767_002576"
                     /pseudo
     CDS             complement(5918..6028)
                     /locus_tag="NK767_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2075 a          932 c         1116 g         2065 t
ORIGIN      
        1 gttgtagatt ttttcttatt tttgggttca tttttcttag acaagatagg tggacatcct
       61 ttcgtttttg agagtccatc gatcccaccc tcacggaatt gacgcatcca actagcgatc
      121 aaattagggt tgttcatgtt aagtgtgttg gcaacttcac gataggacag ctcacttgtt
      181 agatataact ctatcgcatc cagcttaaat tgaacagaat attttttctt ttgtcgcttt
      241 cttactagac cctcttcacc aaattcttga taggcattga tccagcgctt cacttgtgat
      301 ggagccttaa tggaatattt ctttgccaga taatcacttc cgcctttacc atccaagtaa
      361 tcatgaacga tctttaattt aagttcaaaa gtatacttag ccataaaaaa taccgacctc
      421 caaaagttag attttttggt ctaacttttg ggggtcggta caaaacttaa aagttttaga
      481 cccttttatt ttcttaatga cttcagtcag ttttgtgcgt aaagcgtgcg aaacaagaaa
      541 accgcgcgtt tctacgcgtg gaagaagtat ctttagcgat aaaaactcct ttcgatatac
      601 tagtgagtgg ctaccaaccg ctcatcagat cgaaaggagt tttttcatgt caaatgacga
      661 taaaagttta gcatatactc gttggaattg taagtatcat attattttta caccaaaata
      721 tcaacgaaaa gtaatctatg ggcaattacg aaaagatctt ggaaaaatac ttaggaagtt
      781 gtgcgaaatg aaagatgtgg aaatcataga agcacatgca atgctagatc atattcacat
      841 gcttgttcga gtaccaccaa aaatatgtat atccagtttt atggggtatc tgaaaggtcg
      901 tagtgcggta ctaatccacg agcagcacgc aaatctcaaa tataaatacg gaaacaaatc
      961 cttttggtcg aagagatact atgtgagtac agtcggattg aatcaaaaga caaatgaaaa
     1021 atacatccgt gaacaagaag ctgatgatcg cattcgatat attataagta aaagagagta
     1081 cgtagaacca tttaaaaaat aaagagaagt aagtgagtga agatgagcag ttggcggtct
     1141 tttaaaaatc cccttttaag gggcagcgag tgtcgacccc ttctaggggg attaaaaata
     1201 cacccgtcct cacgggtgta tgggttactg atagacttga ttttaactgg tgtcagaaac
     1261 ttatgtccag aaagggtgat gaaattttgg caatttacgg ctattcaaga attagcacaa
     1321 gcaaccaaga ttacaaaaca caaattcaaa cattagaaaa tgctggcgca gaaataatct
     1381 tcctagaaaa tcactggatt atcgaacacc tttggaagta tttttgagtt acgtaagtat
     1441 tgatgatctg tctaacttaa tttgacaatt aagagtatat ttacttcaga tggtaaggca
     1501 acaaaataaa atacaatagg cagatgaact aataaataaa atctgtatta aatacagata
     1561 aattaatata aaaatcaaaa ggatcataaa cttttaacaa gtgatgaaat gggtggaaat
     1621 tgattcttat agacagtttg tctcataaca ttgaagtatt ttgcaatttt agcattgaaa
     1681 cactttttta atcttcaacg ttgttcttat aaacaagatg attgggaatc ttttgaatta
     1741 acactgtata gcttagaaca ccctttatag aaaacattcg cttagcaatc ttaataattc
     1801 cttgtgcccg atgtcgttta gattcagtag gttcctgctc atcgatcatg gaaaaaattt
     1861 gaaggatcag atccttaata aatttattta agagaggatg atccattgct tcatccatac
     1921 tcgataaact agtgataatg agctacacgt ttttctttca agttgatcaa tagcttccac
     1981 gataaaaata tctttagata aaatcaagaa ccgtaggaac tacagggata tttggtcaat
     2041 aagagccacc gctattagta aaaaataagc tatcaagtat gctctcatcc caagaattgc
     2101 ctacttcaat cgttaaaaac cgttaggttt agctaagtgg gggtagttca ctcaatactt
     2161 ttatcaataa tagagcgcaa aactttttct cttaattcaa ctgaatcaat attattgttt
     2221 tgttcataac catgaactaa tagatcacaa atataaagtt gtgatatttt tccagctaaa
     2281 gatccaccat taagaaattc ttcaatggct gtttgtaaaa ctaagtctgc agactttcct
     2341 ataggtgaat gaatatagtt agttatagca ataatttttg cgccattttt tttagctatt
     2401 ttcaatgaat cataagtatc tttcgttttc ccagataaag aaaaaatgat tactaaatca
     2461 ttaactgtta ataaagaagc gacttgggct tgaaaatgag ggtctagtac agcttttgct
     2521 tgaactccta cgcgaagaaa catattttct aaatcaagac tagtatttcc actagatccc
     2581 actccaaata tatatatgtt ttttgattga gtgatcattt taatggcttc attcaatttt
     2641 tcttcgtcta aaagcctaat agtggaatag agagcttctg ttaaactatt agcaatttca
     2701 tcatagtact ttacactagt cggatgttct tttttttgac taaagtcttc ctttgcaatt
     2761 tcaattttta aatcagaaaa accagaaaaa ccaatttttt gacaaaagcg aataatagta
     2821 gcatcgccaa cttttgtgtt tttcttaata tcactcatcg tactataaat aatagttttc
     2881 ccagaattta aaataaaatc agcgactttt ttttcagact tcgttaaaaa tggataattt
     2941 tctttaatta aatagatata actcatacaa actcctctac aatttcaact tttctccatt
     3001 atactccaaa ataataaaaa aaactcctaa atactccatt tttatcttta cttttatgaa
     3061 agcggatgct atattgtttt tggagttgtt atccaaaaat aataaaaaat tggagtgttt
     3121 aggataatgg ataaaaagag gtttttagat cttatttcaa aaggtttgat tgtttcctgt
     3181 caagcattac ctggtgaacc attttacaaa gaggaagaaa gtttaatgcc ttattttgct
     3241 ttggctgctc aaagggctgg agcagttggt atacgtgcaa attctgttcg agatattatt
     3301 gaaattagaa agtgtgtatc attaccaatg attggaatta ttaaaagaga ttatccacca
     3361 gaatctccat tcattactgc cactatgaga gaagtagatg aattagtaga aaccggtgtt
     3421 gaggtaatcg ctcttgattg cactcttaga aatcgacatg atggactatc tataaattct
     3481 tttattacta aaataaaaga aaaatatcct catcagttat ttatggcaga tatttctaca
     3541 tttgaagaag gcaaaaatgc atataaagca ggaatagatt ttgtaggaac tactttaagt
     3601 ggatatactg aggaaagtaa aaaacaagat gggccagata tagatttgat taaaaaattg
     3661 gtagaagaag ggatttgtgt aattgctgag ggaaggattc atactccaga acaagcaaaa
     3721 gagataaaaa aaataggagt tgcaggtatt gttgtgggtg gtgctattac tcgaccacag
     3781 gaaattgcat caagatttgt taaagcatta agttaggggg agaaataaaa tgtttaaaaa
     3841 attttcacaa ttaggacgcg ctttcatgtt accaattgct atattaccag tagccggatt
     3901 actattggga ctgggaggag cgttaacaaa tgagagtgct ataaatgctt atccattttt
     3961 agatcaaccg tggttgcata caattttaag tattatgagt tatgctggaa atgcagtttt
     4021 tgcaaaccta gcactaattt ttgcaattgg tattgctgta ggacttgcaa atggagataa
     4081 aggaacagct ggtttagctg gtggagtagc ttatctagta tatactgcaa ctattagtgg
     4141 gtttcttgaa cttttttcag caaaagatgc aacactagat acaggtgtag tgggatcaat
     4201 tgttgttggt ggtactgttg catttttaca caatcgttat agaaaaattg aattaccaca
     4261 attcttaggt ttttttggag gatcaagatt tattccgatt gtttctagtt tagctgcaat
     4321 cattattggt tctttctttt atttaatttg gccaccaatt caaaatggtt tagtagttgc
     4381 tggtgaacaa attgcccaaa tgggtagttt aggaactttt ttatatggtt ttttattgcg
     4441 tttaacaggt gctgttggac tacaccacac catttaccct ttgttctggt atacatcttt
     4501 aggtggtacc gaaactgtag ctggatcaac aattgctgga gcccaaaata ttttctttgc
     4561 ccaactagct gatccaaacc acactggtct gtttacttat ggtactagat tttttgcagg
     4621 aagatttgca acaatgatgt ttggattacc agcagcaggt tatgcaatgt atcgtgcaat
     4681 tcctaagaaa aatcgtaaaa agaatggtgg gctttatttt agcggggccc ttacatcatt
     4741 tttaactgga attactgaac cagttgaata tatgttttta tttgttgctc cttggcttta
     4801 tgtgattcat gcattcttag atggattatc tttttatttt gctgatatct taaatattcg
     4861 tgtagggaat tccttttcag gtggtttgat cgacttttta ttattcggtg tacttcaagg
     4921 gaatgacaaa actaattggt taaaattaat tccttttggt attgcatggg catttatata
     4981 tttctttgtt tttacattct ttatcaaaaa atttaaggta gctattcctg gaatggaaga
     5041 agaatcagaa gttcattctg aaaaagtaat aaacaaaaat acatcattac atgaagagtc
     5101 tcttatcatt attgaagctc ttggtggaaa agaaaatatt gagtctgttg aagcttgtgc
     5161 aactcgtttg cgtgttgctg ttacgaatgg ggagattatt caaaaacaaa caataaaaga
     5221 cactggtgca actgctgtct tcgaagtgaa gggtggcatc caagctgttt ttggagggaa
     5281 agctgactta ttaagtcaag aaatcaatca aatattagga aaagataatt aggaggtttt
     5341 taatatgttt gaaattttca aaaagaaaaa acaagaaact ttttatgcac cttgttccgg
     5401 aaaaatcatt tccatcacag aagtaaatga tcctgtcttt tcacaaaaat tactaggtga
     5461 tggttttgcc gtcatacctg atggttcaaa aatttatgct ccattggctg gtcgagtaac
     5521 aaatatattt cctactaaac atgctattaa tataaaaagc aactcaggca ttgattattt
     5581 gattcatatt ggaattgata cagttgaatt acaaggagag cctttttcga tttgtgttca
     5641 agaaaatgat gaggttactc ctgaaacctc gttggttgat gtggattttg acaaaataat
     5701 aaaatctggt aaagatactt ctgttatagt tgtttttccg gagaaaaatc agttcaatga
     5761 tttaataata gataatggtc gaattgctca tgggaattca tgtggaaaaa tcacttttta
     5821 aaaaagtgta tataaaaagc tataaaataa gctaaatagg atatttgtca aatgaagttg
     5881 gtgaacagaa agaactgttt tctttctgtt cattttttta ttttattagt aatgattccc
     5941 catgccaaat catgatccgt aatttaaagt tataaaagct tcgaaatcca ttggcatttc
     6001 gttggaatac cttgatatga ttattcaaat ataaaatata gagagaagag agagagggaa
     6061 gatatttata aagggcaaag ttatgtttac tcaacttagg aaatagaaat agatcaattt
     6121 atattctctt gtcaatcgaa tatgaacctt agcactatat accaaatata gtaaaatgct
     6181 gaaagatg
//
LOCUS       DAHUVV010000124         6173 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.127, whole genome shotgun sequence.
ACCESSION   DAHUVV010000124 DAHUVV010000000
VERSION     DAHUVV010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.127"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            725..811
                     /locus_tag="NK767_002577"
     CDS             725..811
                     /locus_tag="NK767_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCH6695523.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="NK767_002578"
     CDS             complement(932..1621)
                     /locus_tag="NK767_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695524.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="NK767_002579"
     CDS             complement(1866..2780)
                     /locus_tag="NK767_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCH6695525.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="NK767_002580"
     CDS             complement(2762..3142)
                     /locus_tag="NK767_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCH6695526.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="NK767_002581"
     CDS             complement(3592..3879)
                     /locus_tag="NK767_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCH6695527.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="NK767_002582"
     CDS             complement(3899..4123)
                     /locus_tag="NK767_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HCH6695528.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="NK767_002583"
     CDS             complement(4578..4748)
                     /locus_tag="NK767_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695529.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="NK767_002584"
     CDS             complement(4763..5296)
                     /locus_tag="NK767_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCH6695530.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="NK767_002585"
     CDS             complement(5317..6159)
                     /locus_tag="NK767_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCH6695531.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAHUVV010000125         6164 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.128, whole genome shotgun sequence.
ACCESSION   DAHUVV010000125 DAHUVV010000000
VERSION     DAHUVV010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.128"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            38..1195
                     /locus_tag="NK767_002586"
     CDS             38..1195
                     /locus_tag="NK767_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6695532.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1221..1661)
                     /locus_tag="NK767_002587"
     CDS             complement(1221..1661)
                     /locus_tag="NK767_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCH6695533.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            1821..2327
                     /locus_tag="NK767_002588"
     CDS             1821..2327
                     /locus_tag="NK767_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCH6695534.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2495..4408
                     /locus_tag="NK767_002589"
     CDS             2495..4408
                     /locus_tag="NK767_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCH6695535.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4413..5414
                     /locus_tag="NK767_002590"
     CDS             4413..5414
                     /locus_tag="NK767_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCH6695536.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHFDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5616..5822)
                     /locus_tag="NK767_002591"
     CDS             complement(5616..5822)
                     /locus_tag="NK767_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HCH6695537.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2287 a          941 c         1217 g         1719 t
ORIGIN      
        1 aatccttaga aaatcttagt caaaaaatta tataagtttg cgtacgattc aaagaaaatt
       61 aagagaactt aatactattt tgaaaaacta tcatttatct tgcagcttga aaaaaggtaa
      121 tccgatagaa ggagaagaat accgtatccg atactttttt cacttgatgt attggcagtc
      181 attcgacgaa aatcattctg atatattatc aagtataaaa aataaacgtc aactattaga
      241 aaaatttgcg aaacatgcgc catatacgcg tagtatagat agaaagaaat ggatcagcct
      301 actaagaatt tcgttgcaac gaatgaaaca aggccactcg attacgtatg tcccagaaga
      361 gatgaacaaa tttgtgcatc cattgataac aaaagaagag tttacgcatt acatactaga
      421 gccttttttt gaagcaaact tctggtcgat gaatcacatc actccaacag agcaagctta
      481 cctttatttt atgtttggtg taatgaacac ctatttggaa gaagatttgt tggatactcc
      541 agataaagta caagaaagtt ggggctggat tgaatcgtct attgtagaaa attttgaaaa
      601 aaacttcaag atttgtttta caacttcaga aaaaaattat ctatatgtca atttggcaat
      661 gatccatttg tattcaaggg tttttgggac aaaaaagaag gtggatgctt ttggaaaatc
      721 agcagaagac agagattttc agcatatatt tccagtgatg tatccaatca tgcaacagtt
      781 ttttagtggg gtagctcgca gaattccaga gcttaatact tattatgaaa agaacagacg
      841 gttgattttt caatactgta tgttagtcag ggatattttt ataaaacacg aacaacctgt
      901 catattctat attcaatcaa agtatggtaa aacacaggaa ttatgggcaa agaaaaggat
      961 attatctatg actgaacgcc caataaaata tagtgcatcc gctgatcagt tacctgatat
     1021 tgtcattagc gattatccta tcgacaaaaa ggcatatgat caaacgaata cacagatatt
     1081 ttactggaat ggacaaccta caaaaaatga ttgggcgcgt ctgaaagctg aaatcataaa
     1141 aatcaggaat gagaaaaata gatccatatt ggaaaaattg gatttcgtta attaagaaaa
     1201 gcagattgtt tatgccaaaa ttaagacatt gcaaaaagat tattttcttt aaaatcgaca
     1261 agatgaccag aaagcaagag ttcgttgagc tcttgctttc ttttagtttt agaaaggaac
     1321 atcctgattt tatcttcgct aaatccagtc aaaagttttg cagattggtc gtagataatc
     1381 ggttttttca ataaagaagg ggatgtacga atctcaatat atagttcact caaactgagt
     1441 tcatctattc tgtgaacaag atttttataa ctaatagaat gagtagagag catttcggtc
     1501 acgccgttgt ttgtcaattt tagtaagtgg ataaattcat ttttggttag tggttgacga
     1561 attaagttct gttcgataaa cggtatatca tggacgataa gccaacgttt tgcgcgtctt
     1621 gaagacgagc agccagaacc tgtatacaat ttaatcatca tacattcctc caatagctaa
     1681 gtatttatga aataataaca tgaagaaata gtcgaaaagt gccatttaga ggtgtaaaat
     1741 aagaaaatgt tgtcacgtat aatggaacta gctagataag aaatactatt aaatcaagaa
     1801 aaaagaaaga aggcacaagt atgtcaaaca cagccgacgc aatagtagtg atcgatttac
     1861 aaaaaggggt atgttttgat tcttcgccga tacacgaatt agaaacagtg acagcattag
     1921 tgaataaaag aatcaaaaca tacgaaacga ttgggaaacc agttattttc gttcaacacg
     1981 aagatgctca attagtgcca caaacaaaac catgggagct attgtcggaa ttatctattc
     2041 ctgagcacag ttattttgtg agaaaaaccc atgcgaattc attttataag actaatttga
     2101 gtgttttatt aactgaattg gactgtcatt cgatcgaatt atgtggggca caagcagagt
     2161 attgtataga tacgactgtg aagtttgctc atggattagg ataccagctt atgatgtgta
     2221 gaggagcaac gacaacttat gataatcctt ttatgaaagc aaaggaaaca atcgcttttt
     2281 acgagaaaat ttgggataaa cgatttctga catttttgga gcaataagtt gtttcttcgt
     2341 ttaaaaaaag cagaatggat actggtttat ccattctgct ttattacgta aaatgagaaa
     2401 ataatcctgt tctattttta tgggataaac catttatttt ttgtataaaa aatgtttgcg
     2461 tttacatata attctcctag aaacataaaa aggagtgaat agcttgagaa caaaacaagt
     2521 attaaaggga gcgttggtca tcttatttgc tgtaagtaca agcgtcccgc aatggacagt
     2581 atatggagag agcatttctt ccgaatcgga atctcgggag gtagaaacca cagaaactac
     2641 tgatgctgga aatgaaacga aatcgagtac ttcacaaaca gaaacaaata ctgaaacaac
     2701 ggttgagaca tctgttacta gcacagatca agaaacgacc gctaccacag ataaccaaca
     2761 aacagaagaa gcaacagcta caaaaatgtc aaacgacgaa tcctcttctg ctaaagtaag
     2821 tgacaaagag aatgggacag aaaatgatga tatcggtaaa ggtgaaaatt tcaatggtaa
     2881 agaatatttg aattacttcg gtaaacagat gacagaaata cttaatggac caacagaaga
     2941 catcaagtca aaattaatag agttattagt cactgagctt gctgttaatg attatggtgt
     3001 agataaagag attatcaaac atctgattgc tacttatcta ttgtacgata tcgcttttgc
     3061 aaaaaacagt gtgttgatgt cgatgcttgg aggtacagat gatgggacaa cgcctacaga
     3121 aaacacaggc ttctatccaa atacaaaaca acttcatcaa actgtactaa acaaacaaga
     3181 tggcaaaatg atggatgtat atgcatatta tgtcgatcaa ggttcagata aaacagtcat
     3241 gatccatggc ggatttcgag ggaattggaa taacgggatc gttacagaag aatacaatga
     3301 tttttataag gccgggtata atttattatt tgttgattcc agagcgactg gtaatagcgg
     3361 aggcgattat gtgacctatg gacaatatga gtcagatgac gtcctttact ggataaatca
     3421 agaagttcga gagcggccat ctcaaaagat tttactttat ggtggttcga tgggagcagc
     3481 tacgatgatg tctgtattag cgaaagatat tcctgtcaat gtcaaaggaa ttattgaaaa
     3541 ttgcggattt gcatcaattg atgaacaact acgttttacg tacagccaga cagtagcccc
     3601 cgcactaccg gatgccataa aaaatcagtt agacatcatt ggggatcaag agcatgaaga
     3661 tttatttatg ggccttttga aacaatacta ttttgatcag gagatgcatt tggatacaaa
     3721 agctgctcta cctacgattg gaatgtcaga ttcattgcct aaattgatta tccatggaac
     3781 agcagatgat gtagtacctg tgtctaacgc gcaaaaatta tatgagttat caggagggta
     3841 taaagatctt ttattagtag aaggagcagg gcatggaaag gcacaggaag ttgaccatgc
     3901 tgcctacacg aaacatgtaa cggacttttt gaaagtagtg ttccatgatc agataaacgt
     3961 aaagtatgtg gatgaaaaca ataaaagtct actcaaagac caggacgaaa tccagcttta
     4021 cggagcttat ggagaaaatt atatgacaga gcaaaagaca ttcgaaggat acgaattagc
     4081 aaatgttgaa ggaccgactg aagggatttt taatgagacg ataccaacta ttattttcta
     4141 ttacaaaaaa atcccagtag ctccaccaaa aaagcaagat ccgacagaga atgcagacaa
     4201 taagggaaag aatgaacctc gagaggatca atcaagtgat gatacgcaac cgattcagat
     4261 gacagtgaaa gtacaaaaaa agacagatgc aaaaaaaaga atgttgccga aaactggaga
     4321 aaagaaacat tcgcttctca tttattcagg tggattttta tctgttagca cgattatcac
     4381 atggatttgg cgaagaaaga gagggtaaat gaatgaaatg gttcaagcga atagtgattg
     4441 cagtaccagt tatcatcgta gttattttag tcactgtatt tttgattaat caaatcacac
     4501 caaagccgat tagcttgctt gttagaaaac aatttgacac aagtgaaaaa gaacaagtat
     4561 atgcccgacc agatgatttc cagcaaaaaa tcgaacaaat cgatataaaa aaagaaatat
     4621 cctatccttc aaagtacaaa aataataata tggatatcta cacaccgaaa gtggaaaaga
     4681 aaaaattacc gattcttttc tggatgcatg gaggcgcata tgtaggaggg gataaaaatg
     4741 attgtcgtga ttatttggaa tatctatgtt cagacacgca gcaaatcatc gtaaatatag
     4801 attatgaacg atcaccggaa gcaaaacatc ctacaccagt tatccaattg aacgaagcca
     4861 ttcaagcagc taaaaaagag ataaaagatc aagcagattg gtcgaatatt tcgattggtg
     4921 gaaattcggc aggtgcacaa attagtggag aatatttgtt agcacttcaa aatgaagaaa
     4981 ttaaaaaagc tgatcatttt gatccgactt tggagaacaa gcagattacg aagtttattt
     5041 ctttgtctgg tctgttggat ccttctgcac ttactcaagt aagtgataaa atctctagct
     5101 ttctttatga aaaatgtgga tgggcttact ttgataataa ggactttgaa caatcagaga
     5161 aggtcaaaag tttagccttg gtcagacatg cggatcgttg gacgcaaaac acattcttaa
     5221 cggatggaaa tacaaatacg tttacgaaac aaatggaaga agtaacttct gtatttgaga
     5281 aagcgggggt gaaagtcaca aaagtagatt atgcggagaa aaatgctgtt ttgaaccatg
     5341 aatatcagtt tgatttcagc aataaacaag cgcaagaaac atatcaaaag ttagtaatgt
     5401 ttttcaagga gtaaagtctt atgatgcaaa agaggttgta atctgtcact tagctccaag
     5461 tcacaaggag caatttcaat aaacaagctt ttcatggttt atgaatattg taacttgtga
     5521 ctgaggagtt agagatggca aggggcacct tttattcgaa atttgggaat atcacacgat
     5581 tattgtcacg atctctttta cgtaaatttt ataagtcaga tttgttgttt tgatcgttta
     5641 aaaaaagcaa caaatccgaa gcctgcggag gcaagaaggg acaaaaagcc gagtggtaag
     5701 caaaagaatg ctggttccac taatattccg ttgttattga tataaggctg ggaacaaaga
     5761 tgaatagcaa ataaaatcaa tgcactagcg aaaaaaagca aagaaatatg gatagctttc
     5821 ataagcagca cctccttttt tataattgta tgataaatag attaagaatc tataaatgtg
     5881 acaagtttgt taggatgtga ataaaatgat catctagcga acttgaaaca aagatggcta
     5941 atgataagca attaatgatt cagcaatcag ataggtacaa agtttaccat catacgtcta
     6001 gaccaataat agaaaatatg ggaatataat agaaaagatt taggataccc ccaaaagaat
     6061 ttccccaaaa attcgatgta tcctaaactt ttttatttta cataacaatc atatcaactg
     6121 tgaaataaaa ttacaaatga taacctaaaa tgaggccaaa agcc
//
LOCUS       DAHUVV010000126         6114 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.129, whole genome shotgun sequence.
ACCESSION   DAHUVV010000126 DAHUVV010000000
VERSION     DAHUVV010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.129"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            62..907
                     /locus_tag="NK767_002592"
     CDS             62..907
                     /locus_tag="NK767_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCH6695538.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1188..2408
                     /locus_tag="NK767_002593"
     CDS             1188..2408
                     /locus_tag="NK767_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HCH6695539.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2405..5125
                     /locus_tag="NK767_002594"
     CDS             2405..5125
                     /locus_tag="NK767_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCH6695540.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5109..6053
                     /locus_tag="NK767_002595"
     CDS             5109..6053
                     /locus_tag="NK767_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCH6695541.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2284 a          959 c         1196 g         1675 t
ORIGIN      
        1 tgttttgtga gaaatttaga cttacaataa gcagtgaatt tataatcaac gtataggaga
       61 catggatgaa atgaaactaa ttggtataga tttagacggt actcttttga attctgacca
      121 gagcattagt caaaaaaatg caagaacaat gaaacattta ccggaagact gttttccatt
      181 catttgttct ggaagagaag tagaagacat taataatatt ctcaaaaaat ctggtttaag
      241 gataccagca gtagggttaa atggttctgt tggatttgat ggagaaaaaa agatatttga
      301 gtcttccttc gataaaaatg atgtaaaaaa aattgattct gtcctatcga aatttccaac
      361 caaaatatat acaaatctcg gaagttatga atctcagaat tataaaaata aaatgaaaaa
      421 aatttttcac gaggtaggga gtgaattttc tatcaaagag ctaaactatg agcttgagta
      481 tgaaaaatct atttgttcaa tcccttataa taatattgag gaaattatag aaaaggatgc
      541 tatcagcatt tacaaatttt ttatttttat tcctaatcgt caattgaaag ctaatatatt
      601 taaccatttg aaaaagatgc taaatgtgtc tgttactgaa tcggcacctg taaatattga
      661 aatcgtacct caaaatgtat ctaaaggtgt ggtgtttgat cgtttggaat caatctacaa
      721 tttaaagaaa cctttgagaa tcgcgatcgg agatagttta aatgacttat ctatgtttga
      781 aagtgcagat attagttttg caatgggaaa tagtcatcaa gaaatcatga aaaaagctga
      841 ttatgtcact gtttcaaatg ataaagacgg agtatctcaa gcatttgaaa gaattcttac
      901 catataaact aattaaggta gtatagaaca tagaattcta aatacggaaa aatggagcat
      961 aaattccagt acaaatagta cttcttcagt gtcaaacgaa gcgaatataa catagatatt
     1021 taggattgtg acaaaaatca tgtcacaatc tttttttcga ataaacttat tttttgaagt
     1081 cggccattta tcgtaatttc tttgcataat acgaacaaat tttcggttat tgatgaaaaa
     1141 aaacattgaa aatggtatga taaaagaaaa aaaggaggaa atttttaatg gtgaaattta
     1201 tccatgcggc agatttacat atggatcggt catttgaagg actgacaact cttgataaaa
     1261 ctgtacagga aaaactattg aagacgaatc tgaccgtttt atcaaatatc attgaacagg
     1321 caataataaa taacgttgat tttgttttac ttgcaggaga taattttcat caaaatcgtc
     1381 cttcattaaa aatacagaaa catttttcag aacagatgaa acggttagaa aagaaccata
     1441 tacctgtatt tataatattc ggaaatcatg atttctatca aaaagagcgg tattggttct
     1501 cctttcctaa aaatgtccat ttatttacat ccgaaacggt agagacaaaa aaaataacga
     1561 taaaaagtgg agaaactgtc tctctcagtg gatttagtta tcgtcaaccg tggattcaaa
     1621 aagacaaagt gatggaattt ccttctagag aactcacaga ttaccatatt gggttgtacc
     1681 acggggaacc tggcgtctct caaaaaggaa actatgctcc tttccaaccc tcaaagatgc
     1741 aagaaaaggg atacgattat tgggctttag ggcatatcca tgtgccaacg gtcttaaatg
     1801 aaaagcaaac aattgtttat ccaggtgcgc ctcaaggtca tactaaaaag gaacaagctt
     1861 ctacatctat tctactagtt gaattaagta aaggaagttg ccaaatacat ccgattaaag
     1921 tagcagaggt ctattggaag acaaaggaaa tatcattgag gaaagcaaga acgacaaaag
     1981 aggtcattgt tcacattcgt cagcagcttt caaaagtaga ggatggattt agtttaattg
     2041 agataaaatt aaaagattat gaccatctca atacagatgt tttggaacgg atacaatcag
     2101 gagagctatt agactatttg ttagaggaat tctcagatag gataaatgat ttctttattt
     2161 ggcgtatttc tctcatagag aatacttttt atacgaagac cccacttgct gcttctgcta
     2221 aattgatgga acagttattt caatactatc agacacctca agatttccaa caaattttaa
     2281 atgaagtata tagtcatcaa gaagcggcta gaatattgag cgaactgccg gaatatcaag
     2341 aagaaacact tgaaaaagcc aagcagctgc ttaatcaaga ttttttattt gaggaggatc
     2401 aggaatgaag attttaagga tcgagattgc tgcttttgga aaatggcgtc aaaaaagttt
     2461 tgacttttat tcaggcaatc agctgatcta tgggggaaac gaagcaggaa aatcaacgat
     2521 ttatcaattt attcaagcaa tattatttgg tttcccttca aaaggccgta aaaaaaagga
     2581 ttatacacca aaagacggct cagcctatgg cggcaaaata tggcttaaac atcctgtata
     2641 tggtgaattt gctgtcgagc gctacaagca gcaaaatcgc ggaaaatcga aggtgtggct
     2701 gggggatcaa gtgggaagtg atgagttact agaaaaactg atcgcacctt tgacgaaaga
     2761 gttattccag caggtattta ctttccagca agagcagttg atggaactag atcatttgag
     2821 agaaaaagag ctacatgatg cgctgatttc tttaggcatc acgggcagtt ctcaagtatt
     2881 ccaaaagcgg caagaatatt atcagaaagc acaacaatta ttcaagaaaa aaggccaaaa
     2941 attactaatc aatcaaaaat taaatgaatg gaaagagctg caagaaaaaa ttcatcaaaa
     3001 acaagaacag gaagcttatt ttggcaagtt gatccaacaa gaacaggaat acgagtcgca
     3061 acagcagcag ttattggcac aattaaaaga ggcgaacaaa caattttcat tagccagaca
     3121 gcaagaactt aatttttctt tatatgaaga atggcaatct ctcaaaaaaa acagcactac
     3181 aggtcttcct gatgagcaac ttcttttaga tctggagtct ttctccaaac ggtatcagca
     3241 aacaaatgag cgtttggaag aattagaagc aacactggaa aagcatagcg ggatggatca
     3301 gcagtcagcc agatattatt tttatttaga gcaggagcag gaactaaaag atctccagca
     3361 acttcaattt acagcggaac aattaaacga agaatggcaa agattacaaa ttgagactag
     3421 agaaacaaat tcagaattga agattttgga acaaaggtgg cattggcaga aaaatccgtt
     3481 accggaagaa tttttatatg aagaagaatg gaagcatctt ctggctgaag aaagaaggat
     3541 tgctgatcta gaaagccaga tttctgtgcg tctccaaata gctaaagaac agcaagagat
     3601 gattgaagca gaagtgaatg acttggaaga gacctatcca gcattattac aaaatcagtc
     3661 agagatagca ccagtctctt catctaaacc gtatatgctg atggctggcg gagcaataat
     3721 cattgcaggt atatttgtac caatgccctt gaaagtgatc cttttactaa ttggtgcagg
     3781 catgattggc tatttcttct acaaaaggca accaaaaatt aaaactaaaa atgatttctc
     3841 tgagataaaa ggaatgtggc aagaaaaact aggacaactt gatctagcct tagctcaatt
     3901 agctgaagca gaagaaaaac aaaagagaac tggagaagaa aaacagattc ttcagcaaga
     3961 agtatatcgt tttgcggatc atcggcactt aggaaagatg gatacgctca aactcatgaa
     4021 agaatatgga caggaagtca tagattatca gaaattatat ggcgtatttg agcggaaaca
     4081 taaaagagaa caggaaatac aagaaaaact acgacaaatc gaccaaaaat ttgagtttgt
     4141 taaagaatgg ttaccgatac aagataagac aatttcagaa aaaatggtat tgctttcccg
     4201 ttttatacaa gagatggaac agatgaaatt tgccagaagc tatcagcaaa atactttatt
     4261 gaagcaagaa atcaatcaat tgaaaaagaa acaaaaagta tggatacaag aatacagcga
     4321 acaattgact caagcaggct tatcttatcc ttcggaagta cctgtctttt tacaaaaagc
     4381 caaacaagcc gaacagcaga tgaaaagata caatgaatta tcggaaatgc ttactccact
     4441 atttcctgaa gagctgacaa aagagaaatt atctgtaaga ctgcgccaac tgaaagaaaa
     4501 gcaatcccaa ctccagcaag aagaaaaaga agcaagtgaa aatcggcaac gactccaact
     4561 tcaagtagaa gagttgcaaa gagacggcac attggacgaa ctttatcaac aagctagccg
     4621 gcaaaaagca aaattacagg aattactgga ggaatgggga gcacttgaaa tcgccgggac
     4681 attcttaggt gatctttcaa cagagatgag tgaaagacaa ttaccccagt tgttaagagc
     4741 agcaggtgat tatttaggta ttttaacaaa aggaaattat cagaagatat tactgaaaaa
     4801 tgatacactg actttaacag acggatatac agaatttccc atctatgagt tatctacagg
     4861 tacgaaagat cagctgatta tggcgatgag atttgcttac ctctcattag aagctaaccg
     4921 ttcgatatgc ccgatcatca ttgatgatgg ctggcttcat tatgatcatc agcgaaagta
     4981 tcaattagct gaacttttag ctaaatttgg agaaaagtat caaataattt gtttttcttc
     5041 tgataaagaa atggtaagct attaccatga gatgaaccaa atcgtaaaac aattagaagg
     5101 agcaaataat gaaaaaaatt cgtgatttag taatcgatga gcagtttgaa caatttgtcc
     5161 tgatcaaaag tgcagatgta agaatcgcta aaaacggaaa aaaatttatt gcttttactt
     5221 tccaagatac ttcaggtact atcgatggta aatactggga tgcttcggag gaggaaatca
     5281 ataaatttac agccggcaca gtcgtttttc taaacggtaa aagagaggtt tatcaaggaa
     5341 accctcaagt aaagattctt catatgcgct taacaagggt agatgagcca ggagagccta
     5401 gtttatacat ggaaagagcc cctttgaaga aagaagaaat ggaagaagaa atcaatcaat
     5461 tgttgtttga gatcaccaat gcgcattgga atcgtattgt tcgctatcta cttaataaat
     5521 atcaaaaaca attttttgag tttccggcag caaaaagaaa ccatcatgca tttgccagtg
     5581 gattagctta tcatactttg acaatgcttc acttagggaa atcaatcgcc gatgaatatc
     5641 ctgagctgaa tcgagcattg ttgtatgctg gtatcatttt gcatgattta ggaaaggtga
     5701 ttgaactttc cggtcctatg tcgacagaat atacgcttgc aggaaatttg atcggtcatc
     5761 tggtattgat tgacgaagaa ataactaaag cctgtcttgc gttgaaaatc agcgaagctg
     5821 acgaagatgt tcttgtatta cgtcatatgg ttttagcaca ccatggctta ttagaatatg
     5881 gctcacctgt acgtccaaga ataatggaag cagaaatatt acatcaaatc gacaatatcg
     5941 atgcttcgat gcagatgatg ttgacttcga ttcgtcaaac agaaccagga aaatacaccg
     6001 atcggatttt cggtatggac aaccgaagtt tctatgtacc aaaagattta tagcaaaaat
     6061 ttattttttt cattcaaaac aaaaaaagcg agacataagt cttcgctttt tttg
//
LOCUS       DAHUVV010000127         5863 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.130, whole genome shotgun sequence.
ACCESSION   DAHUVV010000127 DAHUVV010000000
VERSION     DAHUVV010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.130"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(99..1229)
                     /locus_tag="NK767_002596"
     CDS             complement(99..1229)
                     /locus_tag="NK767_002596"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCH6695542.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="NK767_002597"
     CDS             complement(1878..3080)
                     /locus_tag="NK767_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCH6695543.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="NK767_002598"
     CDS             complement(3140..3343)
                     /locus_tag="NK767_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HCH6695544.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="NK767_002599"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="NK767_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="NK767_002600"
     CDS             4880..5692
                     /locus_tag="NK767_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6695545.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1734 a         1210 c          939 g         1980 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaagctaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttc
//
LOCUS       DAHUVV010000128         5827 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.131, whole genome shotgun sequence.
ACCESSION   DAHUVV010000128 DAHUVV010000000
VERSION     DAHUVV010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.131"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(53..511)
                     /locus_tag="NK767_002601"
     CDS             complement(53..511)
                     /locus_tag="NK767_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCH6695546.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(524..1855)
                     /locus_tag="NK767_002602"
     CDS             complement(524..1855)
                     /locus_tag="NK767_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HCH6695547.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1866..3308)
                     /locus_tag="NK767_002603"
     CDS             complement(1866..3308)
                     /locus_tag="NK767_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCH6695548.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3295..4059)
                     /locus_tag="NK767_002604"
     CDS             complement(3295..4059)
                     /locus_tag="NK767_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HCH6695549.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4163..5038)
                     /locus_tag="NK767_002605"
     CDS             complement(4163..5038)
                     /locus_tag="NK767_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCH6695550.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5293..5377)
                     /locus_tag="NK767_002606"
     tRNA            complement(5293..5377)
                     /locus_tag="NK767_002606"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5341..5343),aa:Leu,seq:caa)
     gene            complement(5395..5466)
                     /locus_tag="NK767_002607"
     tRNA            complement(5395..5466)
                     /locus_tag="NK767_002607"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5432..5434),aa:Gln,seq:ttg)
     gene            complement(5476..5548)
                     /locus_tag="NK767_002608"
     tRNA            complement(5476..5548)
                     /locus_tag="NK767_002608"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5513..5515),aa:His,seq:gtg)
     gene            complement(5610..5694)
                     /locus_tag="NK767_002609"
     tRNA            complement(5610..5694)
                     /locus_tag="NK767_002609"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5658..5660),aa:Leu,seq:caa)
     gene            complement(5715..5785)
                     /locus_tag="NK767_002610"
     tRNA            complement(5715..5785)
                     /locus_tag="NK767_002610"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5751..5753),aa:Cys,seq:gca)
BASE COUNT         1766 a         1247 c          892 g         1922 t
ORIGIN      
        1 ttttgtaaat tcacccataa atatcaccct atttaacttt tttcatcgta cactaaaatt
       61 caaaacaact atctttaatc tttacttctt ggttttgttt taataaattt ccttcaaaaa
      121 tgcctggaga aactacaatg atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat
      181 ctataatagc taacagtgtc ccttctttta cctcctggtt actttcaacc cgtatctgaa
      241 acggacttcc ttcgagttca actgtatcta aacccatatg gatcagtatt tttctgccag
      301 attcactttc gatcgttatg gcatgtcggg taggaaatat attatcgatt attcctttaa
      361 ctggcgcatg gatatatcca tcatgattcg ttatagcgta tccaactcct atcatttttg
      421 aagaaaagac ctcgtctttt actgctgtta atggaataat ttgaccacta gctacagctt
      481 ttagaatgtt tcttgtttta aagaaatgca ctttttatca cctctacttt aatgctggcc
      541 aatatttttt gtttactttg atcatttcat ctagtatttt ttttgcgact ctggcatttg
      601 gtacggtttg attcaatgta aatgcttgta aagctttttg ataagaacct tcaaagtaag
      661 catctactag taacttctct actgcaactt gtgcctccat caatccttta tgaaaatcac
      721 tgattggttt tcttagtgta attggctcca caccagttgc accaacatat gccggtattt
      781 caacaactgc atctgaccgt agattcggaa tcgccccttg attaggaacg attagcataa
      841 agcgacgacg atcatcattc aacaaggaaa tagctatatc gacgatatat tgtccatgct
      901 ccccaaaatt aaagttcaat acttcttcca caataccgcg gcgtatttta tcagccattt
      961 ctttggtatt tttctctcgt ccatccatga ccatattagc acgtgtatac tctttgtcag
     1021 tatgctcaac gaccatatca ggatataagt aatactctaa gtaattattc gggatattac
     1081 ttggaaaatt tttcgtgatg actgacatca tttgaaaggt tttttgccaa gttttatctc
     1141 ctatattaaa atcggcgact tgcatttctt ttgttattag tttttcaata atctctggca
     1201 taatttctcg ttgcaattct ttatcataaa tcgatgtata ccagccaaag tggtttaaac
     1261 cataatacgt aacgatccaa ttcttacgat cataaccgta attcactgca atcgtttctt
     1321 caattgaaat agtcatatca caggcattaa tcatttttac attcggatat ttacgacgaa
     1381 cagcttcaga aacgatcgac tctggatttg tataattcaa aatccatgct ttgggggcat
     1441 agtcttgtat atgatcgaca atttccaata aacctttgat tgagcgcatc ccataagcaa
     1501 atcctccgag tccacacgtt tcttgtccta ctaatccatg tttaagtgga atcttttcgt
     1561 ccttttctcg catctccaat cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg
     1621 taaaagcaag ctttggatca tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt
     1681 tcagcataaa tttaacaatc aagtacatat ccatatttct ttcttcatca atatcatata
     1741 aacgtatttc agataatggc aaccgttttt cattgttcaa aatggcttga acgattcctg
     1801 gtgtatacgt actcccgcct ccagctatag tgataatctg ttttttcatc atcatctttt
     1861 cctttctatt cttctaataa tttattgaat tctgccataa cataagtcac ttctaagccg
     1921 atgataattt gataattttt ttcagttggt ttaatgatcc cgcttatccc taattgcttg
     1981 attcgctgtt cctcgaccat ggtgggatcc actacctcta cccgtaggcg tgtattacaa
     2041 ctatagacat ccaggatatt ggcttttcct ccaaacgctt gtagcatttt tgttgccata
     2101 tattcataat ttttacttgc taaccctaac tctgtttctt ctaaggttac ttcattgcca
     2161 aactctgcct tagcttcacg tcctaatgtc tgaatgtttc tcttattgat caaataataa
     2221 aaagtaacgt aatacaaggc aaaaaagaaa attccgattg gaagaatcaa aatagcattg
     2281 gtagcgattc gaaaattgat gagataatca acaatacatg ctccgaatgt aaaaccaatt
     2341 cgtatattga ataagtaaca gacaatccct gctaaaccgg caaaaagtgc atggatcacg
     2401 tacaacatag gagaaagaaa catgaaacta aattcaacag gctcagtgat atttgcaata
     2461 aatgaagttc ctgcaccgct tcccatgatt cctttgacca tctctttatt ttctttataa
     2521 gctgctttat agatagctag acatatagct ggtataccaa acatcattac tacaaaaaaa
     2581 actgataaaa acagtccagc ctgaggatct ccgttgataa agcgtgtcat ctctcctctg
     2641 aaaacttcct tgctaggaga tgtatagctt cctaaatcgt aataaatata cgtattcaac
     2701 acatggtgca gtccaaaggg gatcagcaaa cgattcaaaa acataaatat ccccacacct
     2761 aatgctccca tatcaacgag cagttttgca aaattatcaa tcccaatttg gatgagcggc
     2821 cagataaagc ccaaaaaaac agctgcgatt gtttgtagaa tcatcaccat agtcaatggg
     2881 aatttttcgc ccgcaaaata agaaaaaact actggtaatt tttgttcttt aaagcgatta
     2941 tacgtccatg ccgctactaa accggaaata attccaccaa aaatccccat attcacttct
     3001 gggtccataa tttctaagcc ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca
     3061 ggtattcctt tgtccttatt tttgacaaaa ccgattgcaa ctcctatagc aaataagata
     3121 tccaagtttc caaaaacggc atttccagct gcaccaatta ctgggatgtt cagcatatcg
     3181 tcagctgata aacgcatgat caatcccgct aatggcatcg ctgcaactgg aagtagcatc
     3241 gctcttccaa gcttctgcac atttgattta acattcattt ttattcctcc tctattaaat
     3301 atcctcatat gttactaact caatcaaagg atcatttttc actttttgag caaatgctga
     3361 atgaattaaa aattcgactt ggtgggtacg aggatacaca aaacttgaac catttaacaa
     3421 ttcctgatcc aaatacgctg cgtgacacat gacttcgatc gttttatctt ctctcaatat
     3481 catgagtaaa cttgccaaat attcttccac actcgtattg atttgtcgtt ttttttcatc
     3541 agtttctcct acgacatcga aacaaggctc aaaataatcg acatgacgaa cttcatcttt
     3601 tcgttcaaag ttgctccgaa ccggcaaatc atattttttt gctaatgtaa caactacctc
     3661 ctgattttca tccaaagtat gagtatgatg atgactgtct aaatgagttg gtatgatccc
     3721 cgcatgataa attttttgaa tctgagcatt ccattcctga tacagttgat ctttgtcaat
     3781 gttaaatgga tgctgataat aagataagga tctgaactct ccgctttcga taagagtatc
     3841 gacagttttg aggagtggtt tgccacacgt caatgttaaa tggactccta cacctagcct
     3901 aggcatttcc tttcttagtt tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt
     3961 agttgatgtc aaaattcctc gctgataagc atccataatc ccataattca caccatggct
     4021 atagccaaaa tcatccgaat taaaaatcac ttttcccatt tcattctcct tcttttttct
     4081 actaaaatag gcgcaaccaa ataatcatag tccatttact atgtgtcatt tggttgcgcc
     4141 tatagatagt tacgccctta ttttaataga ttcttgctag atgaattgcc aaatagatga
     4201 tttcattttc ttgaatggca agttgaaatt gctgttcgat gatatcttta atctttagtg
     4261 ctgtacaata cgctttgcta tgtttaaatg tttcagtaaa aaaaccaata ttttctactg
     4321 gcgtttcttc ctgataaatc aagcgtttaa ccaggaattt gatatgaacc attaagcgat
     4381 gataagtcag tgattcttta tcataaacgt ttgaatagga tttttctaga tgggacaaaa
     4441 tcgtggtcgt catttctact atttttaagc tctttaaact attaggctct cctagctcag
     4501 cattaatgat atgcatagtg atgaatccta cttcatttga ccctaactcg gtttgatatc
     4561 gatgattgat tcgctcgacc acttctactg ctatatcata ttcatttgga taaaggaatc
     4621 tcatttctgg caacataatt gctgtgacat ctctatcttc tctttttcgt tgaatagcaa
     4681 aataaatatg atctgtcaat gtaatataga tatttgaatc taatggagag gagatcctag
     4741 ctgttatcag ttcaatcact tcttccgcaa aatccataaa ttcgattgga atctgcgaga
     4801 ctatctcact gaatcgtttg atcttttctt tagaatctaa tacaaataat ttgtcagcat
     4861 cctcaacatc aaatggctta ctcactttag cagaaaagcc aatccctttc cccatcgcaa
     4921 ttttttctat tccatcgtca tcgatcaata cagcattatg attcagcttt ttcatcacat
     4981 acaccgttaa ttcacctcct ctatatgata aaacaaaaca acttgcagac tccttcatac
     5041 atatttttgt ctccaaacaa tttttaaaga caaaaaacga cctaagaaag aaccctttct
     5101 taggtcgtgc ctaattgaat agtagcaccc tacataattt acaaaaatag tttaacacat
     5161 aataaaagcg ttatcaatat cttttttact cgttttatga taatacttgt atcgaattga
     5221 tttggaggta attacataaa aacaaaaaaa atatagcaac tctctataag aaagttgcta
     5281 tattgataat tataccggcg gccggggtcg aaccggcacg tcctcaacgg acactggatt
     5341 ttgagtccag cgcgtctgcc aattccgcca cgccggcaaa tttaaaaata ataactgggg
     5401 tagctggatt cgaaccaacg catgagggag tcaaagtccc ttgccttacc gcttggctat
     5461 accccaagaa taaagggcga atgatgggaa tcgaacccac gaatgccgga gccacaatcc
     5521 ggtgcgttaa ccacttcgcc acattcgcca tcataaaaga tgttatttaa tttctcaata
     5581 aaaaactaag atgaagacat cttagtcaat accggcggcc ggggtcgaac cggcacgccc
     5641 tcaacgggca ctggattttg agtccagcgc gtctgccaat tccgccacgc cggcaagttg
     5701 atttattagt actaaggcgg taaccggatt tgaaccggtg atgaaggttt tgcagacctc
     5761 tgccttacca cttggctata ccgccattat actattatca atctataaaa taaagaataa
     5821 attatag
//
LOCUS       DAHUVV010000129         5797 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.132, whole genome shotgun sequence.
ACCESSION   DAHUVV010000129 DAHUVV010000000
VERSION     DAHUVV010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.132"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..259)
                     /locus_tag="NK767_002611"
     CDS             complement(<1..259)
                     /locus_tag="NK767_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCH6695551.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSL"
     gene            complement(400..1002)
                     /locus_tag="NK767_002612"
     CDS             complement(400..1002)
                     /locus_tag="NK767_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCH6695552.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            1247..1453
                     /locus_tag="NK767_002613"
     CDS             1247..1453
                     /locus_tag="NK767_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695553.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            1765..2484
                     /locus_tag="NK767_002614"
     CDS             1765..2484
                     /locus_tag="NK767_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCH6695554.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(2565..3248)
                     /locus_tag="NK767_002615"
     CDS             complement(2565..3248)
                     /locus_tag="NK767_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HCH6695555.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            3466..5652
                     /locus_tag="NK767_002616"
     CDS             3466..5652
                     /locus_tag="NK767_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCH6695556.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2170 a          971 c         1110 g         1546 t
ORIGIN      
        1 aaagcgacgt caataaccca aaaggaagcg cgtaaaccaa ttgttgacgc gaagtcgaaa
       61 agaagaaaaa agtaagcatc agcattccta tagcaattag agcagtaaaa atccctaaaa
      121 tcgaatagac ccatccaatc aaaatacaag aaatcagaat gactcccgca aacagatatt
      181 tgtatcggat gtgatggtca atcaataaga tacaaattgt gtaaatcaaa tattgcaacg
      241 ctatggacca taaaaacata aagtacagct cccattgatt tttaccaaat tatagcacaa
      301 ttggcgtatt atttttttaa tttttcaggg attttgaatt cagaactcat accaaagcag
      361 cagccagata caaaaaagtc agataagtta gttaattgtt taatgattgt ttttttcatg
      421 aaagattcct ccaaaattca tcaagtagtt tgtaatagga agcaacgtag ctatttctaa
      481 aacaatccca agtagcaaat aggtagaaaa gtaagaagct gagaaaaata acgtaatgag
      541 agaaaaaatt agaaacatgc tgatcgcctt ctttaataat gggggacgtt tctcgggttc
      601 aatgggattg attgctgtac ctcttgatcc atagcgaagc agtaacaaaa agcagctgat
      661 taacaaaaag accatcattg ggaaggagat agtcaattgc agatgtttca tcaccaaggg
      721 aacgccccat aaataaagta gtgtccacag cagacaaacg aattcagagt cggaatgcaa
      781 tccataagca tatctgcgga tgcagatata agaaagaaag gtgattcctg tttccgccca
      841 tgagcctgtc accagagaaa agactagaac gaccgctagt tttccaagat tattcaataa
      901 aacttctaag acaaatttta cttggacata caccatatct tcttccgatt tgtctccgct
      961 gaataaggct tctaataatt tgttcacaac tttatctttc atctcatacg tacctccacc
     1021 acaaatttta tgctaaaaaa aataaaaaac gtcttttgtt tcataaaaag tggttttgac
     1081 atcaaaaccg tcatgatctc ctaaaaaaat cttcgttttc caagaacctt accaaaaaaa
     1141 gtgacagttt tgatacgaaa agtacttttt atgacaagtt tgtgcttttt cgagtgattt
     1201 atgttaaaaa attactgacc ttataaaagg tgatggaggg attcttatgg aaaatgaaca
     1261 attgtttgaa gtcggcgaaa catatgcatt taaatctgat ttatttgaat caccaataaa
     1321 aggaaaaata gaaaaaatct atgaaaatag tgtgatgata cgtgtcatga aatgcgcagt
     1381 agaagacaat gataccgctc ataatctgca ctggcagtta gttgtacgca aatcaagtac
     1441 aatagaagaa taaaaacaga taaaatgttg agagcataaa gaggttgtga gtctcccgtt
     1501 tagctctgag ttacaaggaa taatgttaaa aaacagcttt tcatgtttta tgaatattgc
     1561 cttgtgaccg aggtgttggc aggagaatac tgtttatttg aaaaaagggc tgcgacaaga
     1621 cttttgtcac agcctttatt ttcatgaata ggagttactt ttggctactt ttagagaagt
     1681 agcttttaag gagcagataa agtcaaggct tttatttcta taaatgaagt agtaaaatga
     1741 agaacagatt ttattacagg aaggatgccg ataattgtga tcattgttgc aggaacgatc
     1801 ggtgcaggga aaagcacact aactgaaatg cttgcccaag atttagaaac aaaacctttt
     1861 tatgagaacg tggaagataa tgaagtattg ccgctatttt attctaatcc agaaaagtat
     1921 acttttttat tacagatttt ctttttgaac aaacgctttt tggcaatcaa agatgctttc
     1981 agccatgatg acaacgtact agaccgctcc atctacgaag atagtatgct gtttcatttg
     2041 aatgcagatt tagggcgtgt ttctgaagta gaagtaaagc agtatgaagg tcttttagaa
     2101 acgatgctga aggagttaga agaaatttcg ccacaaaaga aaccagatct actggtttat
     2161 attcgtgtat cctttgaaac tatgttagcg agaatcaaaa aaagaggccg ggaatatgaa
     2221 cagctagaac aagatcctga actctattcc tactataaag aactcaatcg acgatacgaa
     2281 gagtggtatg aacagtttga tatatgtccc aaaatagtga tcgatgggga caaatatgat
     2341 ttcgtagccg atccggcatg tggagaacaa atcgtacaag aaatcaagat gcgggcaaag
     2401 aaaatgacag aagaggcaga tgcccagtcg atccgctaca ctacaaataa cccgaaagca
     2461 aagggaatct ttacaactaa ataatataaa aactaagcaa attgctcact cgcagatatt
     2521 tctctgtgag cagcatcttg cttagttttt ttgattcatt gagatcaaat agtgaaccga
     2581 ttcgtatgat aaaattgcca taaatgaaat gtctgagtga gggccatgat caaaaataaa
     2641 atggaaacga tcaaaatcga atgggcgcca aacagatgat gacagaaaac aaggacaata
     2701 gctcctgcga taaaggcaag aacgacactt agatagtgga aaaatcgatc aaaatctgct
     2761 ttttctttag aaatacaata atcatataag ttcgttgcta ccccttttaa atttccagtt
     2821 gtaaataaat tagtgtaggc acgcccgttg attttatcaa aagctaccca ctggatcgat
     2881 gcagtaaaag caatcaaaat cgttactaat gcgggatgct catggacggg tgtcaaacta
     2941 acaaagaaaa aaatcactgc ttcataatat agaacaaaca agcgccagaa atgtttttct
     3001 ttttttctaa aataatcaat tagatacttt gttagaagaa tccctaagaa aaagaaaagg
     3061 gtagacaata cttttttacc aacacttagc cattctccgt caaatgcttg gatgatcgat
     3121 agaatgatat tgccagtttg agcagaggca aaagcaccat attggatata agtataactg
     3181 tccatggctc ctccgataaa tgtcagcaaa agtcctattg tacgcccctc gtgaaaggaa
     3241 gtctgcattt ttttcatcac cttttatgaa tatttttgtc ttccttatct tagcataaac
     3301 gattgaaaaa aaggaaaaat tttcagaaaa ctaaaatcat atcgcttccc attttcattt
     3361 ttagaaaggt ctgttagtat aaagaaaaaa tagacaacga aactttaaat aaccaacttg
     3421 aaataaacaa attcaaagaa gaaagatggg aaggaaatca atcagttgaa tgaaaaagaa
     3481 attctccgat tacaaaaaga attgaaacgc tgggagaaca agtataaaaa gcttcaacaa
     3541 gtctttcggc tacaagaaga aatcattgct acctatgaag cggcttatgg cgaagcaccc
     3601 ggacttattg aggaagaagt acaagtaaaa gaaagtgaag gaaacagctc tttctttaaa
     3661 atgatggaga aacgttccta taatcgcttc aatcaagaac aaaaagcagc tatcacatat
     3721 gatatgaaga aacatcttcg aatcatagca ggagctggga gcggcaaaac gcaaacaatc
     3781 tgtgcaaaag ctgtttatct gatgacgcaa aaacaagtag acgaagaacg catactaatg
     3841 atcactttta caaggaacgc tgcaaatgaa ctaaaaaaac gagtagataa tttcagccag
     3901 agaaaaaccg ctgtccatat cggaacattc catagtatct tttttaggat atacaatgaa
     3961 atttgtcgaa aatttcctga agtagcaatg caaggaatac aaggagactt ttcaaaggat
     4021 actgcacaaa aagtgaatgc tgttttgcaa cagctgatac gtaaatataa tctttatata
     4081 tttgatcaat atggagaaaa aacgattgct tctcggttag actattggca gaatatgaac
     4141 ctttcagtag aagaaatggt tcagttagtt aaagaaaaat atgattccat tgataagaat
     4201 gcacgacagc cgatatcaga acgactgtat ggattgctca cggaactcca agaacaaaaa
     4261 agaagccagc aattgctaga gttcaatgat gtactccaaa atctgaaaag cgctttggag
     4321 aatgaggaga tccagcatta tatcggccaa aaatatgatt atctattcat tgatgaattt
     4381 caagatacta atccacttca atgggaaatc gtgaaacgga tgacaaagga aaacaggatc
     4441 aagttgattg ttgttggaga tgatgatcag agtatctatg gattcagagg atcagagcca
     4501 gcatacatca agaactttga aaaagaatat cctaccaaaa ctcttttctt attaactaat
     4561 tatcgttctc gggcagctat tgtccaagga gcaaatagat tgatcagcta taataaaaat
     4621 gatcggatac caaaggcgat gattccagca caaaaagaag caggtgttat ggaagcctat
     4681 ttgttttctg atactaaagc agaaagtcag tggctaatca aacaaattca gacatttatt
     4741 caaaaaaatg ggaaatataa ggaatcaatc atattatacc gatcagcttc acaaacgcag
     4801 cagcttgtcc aatctctgct gaaaacgaat attccttttg tactggaagc agatagtcca
     4861 tatgaaggta tctttggtat caagtctttc caacatttct atcaaaaaat tgttcattgg
     4921 caaacagcac tgaatctgca aaagaaaaat caggcctacc agcaattgct ccggcagctg
     4981 atggcagact gctacctgaa aaaaagtgaa tgcgatcaat acttttctcc taaatatcaa
     5041 aatatagccg ttgcagatta tattttgtcg ataagcccca atttaaaatc gaaatctgca
     5101 gaaatccgag agtttgagga agcgttgcgt aaaacagacc agcatcaaga aatctattcg
     5161 cttgttcaaa gctatcttca tctgccacgt atagctaagg aaatcgacca gagtgatcaa
     5221 gaatggatac gagaagaagt aaagcagcat gctactttca gaagactcca agccttatcg
     5281 caagaaaccc aagcgacagc taaagaatta aaaacccgct tgattgctta cagacaagga
     5341 aaacttgatg cactatgtat ccagagcatt cacaaaagta aaggtcttag ttaccgaaat
     5401 gtctttttga ttggttgtaa cgaaggaagt ttgccttata acggagcgac agaaaagaaa
     5461 gtaatcgact ttaatgaaat aaaagcagaa ccagtaacga cgatagaaga agaacgaaga
     5521 ctattttatg ttgcgatgac tagagcaaga aatcgtctgt atctatgtgt acctcaaatg
     5581 aaaaattcaa gaaaattgaa agtatcgaga tttatcaaag aaaccgggag tcgtgtgaaa
     5641 aaaggaaagt gatagaagag agggtgtgac agaagtggtc agtttcaaga aagaagaagg
     5701 aatgtacgaa aattgcttct caaatttttg tgaaatttca gcttattttc gaagaagcta
     5761 tttctggaac acagtttatt cggaaaaggg aggtgtg
//
LOCUS       DAHUVV010000130         5691 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.133, whole genome shotgun sequence.
ACCESSION   DAHUVV010000130 DAHUVV010000000
VERSION     DAHUVV010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.133"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..1128)
                     /locus_tag="NK767_002617"
     CDS             complement(<1..1128)
                     /locus_tag="NK767_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCH6695557.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
     gene            complement(1164..1874)
                     /locus_tag="NK767_002618"
     CDS             complement(1164..1874)
                     /locus_tag="NK767_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCH6695558.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1942..2757)
                     /locus_tag="NK767_002619"
     CDS             complement(1942..2757)
                     /locus_tag="NK767_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCH6695559.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            complement(2768..3736)
                     /locus_tag="NK767_002620"
     CDS             complement(2768..3736)
                     /locus_tag="NK767_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCH6695560.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            4499..>5691
                     /locus_tag="NK767_002621"
     CDS             4499..>5691
                     /locus_tag="NK767_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCH6695561.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLN"
BASE COUNT         1751 a         1169 c          908 g         1863 t
ORIGIN      
        1 attttttata acagttgaca tgtaagcctc ttctaacgtt tgattcaagc cgagaatttg
       61 tggatatacc tctgctacta cttgtccttc gttcattttt tctgctgtct tttgagtaac
      121 catttccaaa tcactaatcg aattaaaaac gccttcaggt aaccgctttc caaattcgac
      181 aagaattgac gcagctttat aacccgcttt atctccttcc gttgtcaaac agcaaggaat
      241 ctctacttca ttgaatgcag caacttctgc tttaattgcc tcatagtcaa tctcgggtga
      301 agctactcct gactgataaa caccgtaaat atctccactt ctatccgcaa caacaccatc
      361 taccatgatc ttcactccac caaagcggac cttttcaggg aaaacttttt ccttatactc
      421 atatagaagt tcatgctgta gaggttcttg gactgcctgc acatataggt tactaagtat
      481 tttcctctga ggtatattag aaagaaattc tgcgtcatca aaaacgattt ctttcattga
      541 tataacacct tttgaaagca gtaatgcttc aaattcagcg taaacctctg ctaaaagctt
      601 tttgtttgct agcatttcct taattaataa tactcgactt tcagcactgg tttctttttc
      661 tgtaaaacca taagtctgct tagcaatagc attcatccaa caatggctgc gatttttgtc
      721 gatcgctgtg attggaccaa agtggtttat ttcattcaat agctcttctg atgggtcagt
      781 tttccacagg gcagcatccc agccatgagc ataaattggt tcatctgctt ttttatggtt
      841 cattacagct ttaatacgct ctacagcctc gttagctgaa gatgtatcgc ttaaatctat
      901 gccagcattc atagccaaat aaccactgaa aaatacatga ttatcatgaa ttgctggcat
      961 aacgaattga tcatcataag caattaattc cgtgttttca tttaatagat cactgtaatt
     1021 ccagttgttt tccacctttt caatgatccc ttcattgatt acaatataac ctttgaaagg
     1081 tggctgctct ccatcaaaaa tcatttttga ccgcaatatt tgcttcataa ataaaatcta
     1141 ctccttgaaa tccaaacagg aaatcataag taaaaacgat atacttctcc caggtaatat
     1201 tgattaccta catgaattgg tatgttgttt tgatcgttga ttaaagtatc cataaagaaa
     1261 aagggcgtcc caacagctac ttccattatt ttggcaattt ggtcatcagc tgtgacaatt
     1321 tcaagtgcag tttccccagg attgatggct aaggtacctt ggactttaag tacttcatac
     1381 aaagaatcag tcagctcttc ctccattaag aaactatatc tttcataaga aaaataattg
     1441 ttttctaaca taatggggat gttatccgcc attcgctttc tctggatata taaaacatca
     1501 tcaccttgag cgatatcgag tttttttgcc atcccttcag ttgctggaat gatcttgcgt
     1561 tccaaaacaa tgctagtcgg tgtaaaacca ttagatttac aactctcact gaaacccata
     1621 atatattcaa ttttacgaaa aactttatgc cggttgacaa aagttccttt cccttgtttt
     1681 ttaagtaggt acccttcttc taccagttct tctactgctt ttctcactgt gattcggctg
     1741 acctcataac tttctatcag ctctgattca gcgggaattt tcgtattctc gggatactcc
     1801 cctgtattga ttttattttt taatatttct tttaattgta agtacaatgg taattgtgca
     1861 tttgtatcca gcattttttt aacctccact ttaccgggat ttctttcttt aggttatcaa
     1921 atgcaggaat ttaaaacaag ctcaatcgta atgaatacca aagccgaacg aaccgtcaac
     1981 cagacaagac ttcccagcaa attcattccc ctttttcatc gcggtgacta tatcgtcccc
     2041 ttcgagaaaa taaagtagga aagctgttaa gaaagaatct cccgctgcca ttgtatcaac
     2101 tggttcttct agcacggggt tcacttcata aaactttgct ccatcataac caatagccgt
     2161 tttttctcct cgggttgcaa tcaatacatc atttcgctca caaaaatgct tcatcaaaaa
     2221 ctgctttact tcttcatcgg aaaagtcttc tatagaaaag aagccaaaat caactttatc
     2281 aattgcccgc tgaacctctt cttccgtaaa gtcgtcagaa aaatcataag aaatctttcc
     2341 agaaacattt tgtaactctt ctaatagatg attgacatgg ctatataatc ccatatgaat
     2401 caattcaaac tggtttaaat attctctgtc ttcttcagta agctgaatcg ggtattcacg
     2461 agtaacacct cccgaattgc tgccaaggaa aatccggtcg ccctcatcaa tcttcactct
     2521 ggcacagcca ttctcgcctt tttcaaagcg agagtgactg ttgtccagtc ctatttcttt
     2581 tattgctgat aaaacatgag ctgcctcctc atctgagcca aaaactccca taaagctact
     2641 ttcatgtccc agtttttttg caaaaactgc aaaattcaat gaatttcctc caggatacat
     2701 aattcccaga ttttcatatt tatcgacgac gttatcacct aagcctaata ccttcatttt
     2761 aatcctccta gtactccact ttccccatat agcgacgaac atctaaatca tgattgcgta
     2821 catctgctaa agctgaacga tattctgaaa gaatactgta gaataaaatg ggattgaaga
     2881 attcagaaac ttcatctgat atcatatcca atcctagttc ctttgcatca acgatttcta
     2941 ctctttctgc atatttatca aggaatcgtt ttgcccgttc atctaaagca cgagtgcgtc
     3001 caatgctcat caagagaata aaattcgtat ctttgtctgt tacttcaaaa ggtccatgga
     3061 agaattctcc agaatgaaca gaagttgaat caagccattg catttccatc aaagagcaaa
     3121 ttgaaaaacc ataagcatgt ccatatgatg gtccactacc taagatataa aacattgttt
     3181 catctttgta cttatcagca aacactttaa tacgatcctg cacctgctta atgccatttt
     3241 caattacggt atcgataacc ttcatgcctt ctctgaattt accgtaatgt tcaaatgttt
     3301 cggtaacatt gaccaaagat acagttaaat caagaatgat ggccatagga ttattgacta
     3361 tttttgcatc atctccccat tcgtaaagga aactatgatc agaaaccttt aataaatccg
     3421 cagttttatt atgagtcaga ccaacagtca cagctcctct agcttgagcg acttctcctg
     3481 cttttaccgt ttctgctgta tttcctccat gagaacatag aatgacaaca gatttatcat
     3541 ctaattgctt tggggttaca tggttaaatt cgttcgctgt atataagcct gtaatacact
     3601 tcgatgcttc ggctttaaga aaataatctg agacatacat atctaccagc gaaccgccac
     3661 aagccaccaa ataaattcca gaaatctctc catcattttt ttctacaatt gcgttaaccg
     3721 tttttgctac atccatcaac atttcctcct taaaatttta aaaatgttaa ttttatccaa
     3781 aataaaagta actgattaaa tgtaaagata tcacataacg tcatataacg tcatgtgaca
     3841 taattgcatt gtatccgctt tctttgtgtt tgtcaatagg aaaaatttat ttctctaaaa
     3901 aatataaata atctgcagtg aaacttgagt acatatacta atttcgaaaa atgcctatga
     3961 tttaaataat tatggtgctg ggatgaaggt ttataagttt gtcttgattt gtttagaaga
     4021 aatcgttttt gcacacagtt gaattaggaa actactggag tggaaaataa agtgatttga
     4081 cgggtagatg ggtgcactta aatttgaaaa tggcttaaaa tgctttattt tgaattgcag
     4141 aatttctttt agtacgcaat ttatggtctt aaaactgatt aaaaagtgat acattgattt
     4201 taatacaatt tatgatacaa aaaatgacct cctttcttaa ataaaagagg tcattttgtt
     4261 ttgtctcata atcgattgtt tttgatacag tagttatatt cagaaaaaat aaagactctt
     4321 ctgagtttat aatcctttgg attatatacg gatacagatg tagacataaa acaagctcaa
     4381 ttttcaaaaa tggcattact cacctttttg gtgaattatt tgtataaaaa aaatatagaa
     4441 aagacaccct gaaatccttt agaattaatt tggctaccaa ttcataggag gattcaagat
     4501 gtctcatact cagcttatca aagataccct aaatattcta gacctaaata tctatttcga
     4561 ggaaaattgt ttaactaaag aaaaatacaa agggcaaatt tgcttgattt acaaaggaac
     4621 tcttctgtac aagccagaag agtgttccca ttgtctttgt gtcgtcccct ctaggatcat
     4681 tcgttggggg acgacaaccg ttcggttgtt actgaatgat gtgtctgagt atcgcactta
     4741 tctggagctg aagaaacaac gatttaaatg taagtcttgc caaagaacat tcgtagcgga
     4801 tacctctgtt gctgaaaaac actgtttcat tagtcaaaaa gttcgttggt ctgtcattgc
     4861 tcgcttaaaa gagaatactt ccatgacgga aatcgctcgt cagaaaaata tttccacttc
     4921 ttcggtctat cgtgtcatga agcgattcta ccgcccattg aatcctttca aacagacttt
     4981 gccaaaggtc ttatgctttg atgaattcaa atcggttcga agtgttgcca gtgcaatgag
     5041 ttttatcatg atggacggac aaagccatgc attattggat attgtcgaaa atcgacgctt
     5101 gccttattta gaacgctatt tttctcggtt ttcattagcg actcgtgaag ccgttcaatt
     5161 gattgttatt gatatgtatg cgccttatgt ttcgttggtg aaaaagcttt tcccgaatgc
     5221 tcagttgatc atcgatcgtt tccacatcgt ccaacatatt ggcagaactt tcttgaatca
     5281 tcgagtgaaa gaaaccacgg tacgacttaa aggaaattcc cactctcaac gagggttagg
     5341 gaaaaaattg aagcgctatt ggaaactcct acaaaaagaa gaagcaaagt taaactatga
     5401 aaaacgggtt tggcgtgcca gttttaaaga ttatttgact gaaagtgaga ttgccgatcg
     5461 actgttagct gcttgtccca acttacgcca aggctatcag ttataccaag acattcttta
     5521 tgcggtaaag aaaagagatc agtcactatt tgaggattgt ttaactagag acattagcgg
     5581 tctgccagaa acctatacaa ccactttacg aacgtttaaa aagtttctgc cacagatcca
     5641 aaatgcgctt cactacagct attcaaacgg accattggaa tgcttaaaca a
//
LOCUS       DAHUVV010000131         5597 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.134, whole genome shotgun sequence.
ACCESSION   DAHUVV010000131 DAHUVV010000000
VERSION     DAHUVV010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.134"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(146..625)
                     /locus_tag="NK767_002622"
     CDS             complement(146..625)
                     /locus_tag="NK767_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCH6695562.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(641..1393)
                     /locus_tag="NK767_002623"
     CDS             complement(641..1393)
                     /locus_tag="NK767_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCH6695563.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            1510..1580
                     /locus_tag="NK767_002624"
     tRNA            1510..1580
                     /locus_tag="NK767_002624"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1542..1544,aa:Gly,seq:ccc)
     gene            1946..2671
                     /locus_tag="NK767_002625"
     CDS             1946..2671
                     /locus_tag="NK767_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCH6695564.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            2925..3191
                     /locus_tag="NK767_002626"
     CDS             2925..3191
                     /locus_tag="NK767_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCH6695565.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            3188..3619
                     /gene="ruvX"
                     /locus_tag="NK767_002627"
     CDS             3188..3619
                     /gene="ruvX"
                     /locus_tag="NK767_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCH6695566.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            3645..3950
                     /locus_tag="NK767_002628"
     CDS             3645..3950
                     /locus_tag="NK767_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCH6695567.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(4035..5180)
                     /locus_tag="NK767_002629"
     CDS             complement(4035..5180)
                     /locus_tag="NK767_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCH6695568.1"
                     /translation="MATIKQYEKKDGTKAWLFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKKETNETFKEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTASRIFKYAMTLGIITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLESLFQYLESKRSTYRADYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(5231..>5597)
                     /locus_tag="NK767_002630"
     CDS             complement(5231..>5597)
                     /locus_tag="NK767_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCH6695569.1"
                     /translation="DYCLDIIELDLEDFINKRHSISSDKFKNRFLSSFHEGVSNSGYK
                     YGDTDLIYKYGIALLEKFDDSYKLNNPYESIDAYFSAMTVNTEDTEKLIYTICKSLGI
                     PFQKGSNTAQILSKNHNNF"
BASE COUNT         1801 a          979 c         1098 g         1719 t
ORIGIN      
        1 agaagctatt tttacaaaac tttttttaat actcataatt acttcttatt gctcctgcgg
       61 ttacttgtcg caatgtttgt gagatatttt gtcatcgctc aaggctgaac gtctctttca
      121 caaccttttc tttgttacac ttcgtttatt taaaaagccg tttttttcgt ttcatcatga
      181 ttgcgtcacg ctttactttg aaattacgga tgttatggtt aaagacttct gataatacaa
      241 gacctaaagc gattgctcca gcaatcataa ttgcttggac cgcataatga gccgcttctc
      301 gataggattc agtaacgaga tttcgaaccg cttgataagc taaaccacca gggactagag
      361 gaaccatccc gggaatattg aaaatcgtta cgggcatttt caaacgttta gagaaaagat
      421 cgctgatgaa tgctactcct aaagaaccaa gcaatgaacc aaaagcagca gatccgccta
      481 tttgaacaca gatccagtag atcatccagc caaaagcacc agaaagacca caacaaatca
      541 atgaacgcct aggtacattt gtaataacgg caaatgccgc agtcgctaaa aaactaaaag
      601 aaaactgaat aaatatcgtc cccatacttg tgttcctttc ctaataaaaa agctggaaaa
      661 taaaagcaat cgcaaaaccg atcatcgtag cagtcatcat cgcttctgtt ccgcgagaaa
      721 ctcctgagac ataatgtcca gctagcaaat cacggatagc gttcgtaatt tgaactccag
      781 gaactaaagg catcacacat ccgataatga ttagatcctg attgcttcct aagccaaaac
      841 gtacgctgta gatagcggct atgccaatca ataaagaaga taaaaattcc gataaaaatt
      901 tgattctaaa aaatttcaaa ctatatagat aagctgcgta tcccaatcct ccaatgacta
      961 aagtcacagg taaatcggtc atctttccgc caaataacac cataattgtt ccactgatca
     1021 ctgccgcgct aacgatttgc aaccagacag gaaagaaaga acggtctcgg tcgattgctt
     1081 gcagttcgca gtaaagttct tctaatgtta gctctgaagc aacatattta cgtgaaagat
     1141 catttacttt tactactttt tctaaattaa tcgaacggtt ggtaatttgt tccattcgga
     1201 tggtcgaggt acgatcaaga ccaataaaga gaccggtttg agtgacatag ctgaccaaac
     1261 ggaaatttcc agaagctaaa gcaattcgac tcatcgtatc ttctacccga tacatctccg
     1321 cattgctttc catcatgatt ttcccagcga gtaaacaagt gtccagcaac agatctaatt
     1381 cttttcccgt catagcatct tctccaataa ttgattgatt agttttattt tactacaaaa
     1441 catatcggaa gcgtaaagaa taaaaaaact tgaggaagga gtgaaagagg cgtatactgt
     1501 ttttgtatcg cgggtgtagt ttagtggtaa aatcccagct tcccaagctg atgtcgcggg
     1561 ttcgattccc gtcacccgct taaaagaggc tgtgaaaaag ctgccctgca tcaagtaata
     1621 agaatagtca agcaaaaaag cttcttatgt ttttgacttg gttggactta ttaccgcaga
     1681 tgcaagcttt tgaacactgt ttaccaagag gtcgtacaga agccgtttag ttttgagtat
     1741 taagaaaaat tttttgaatg ataataaata ccttgtaaat agtgttttgt gattgagtag
     1801 agacgatgaa tccgtctctt tttttgtatt cttaagccgt ctaaaaactg gttttatttt
     1861 tttactgttt ctatttcttt ccagtattta tttatagggg aacatatgtt aagatttagg
     1921 gaactgttag taaaggagta aagaaatgag caaaaaaaca accaaggcga tcgtggggat
     1981 cgtcgctgct gtgattgcag gggcactcgg tatttccacc acacaaaaag attcagatat
     2041 cttccaacaa attacaacca tttttgattc cacaccgcaa gcttctcaag tagcggatgt
     2101 gccagaatat gatggaaaac atcaagaaat agaaatcaat gggaatcagc cggagtttac
     2161 aaaagaggat cttagtcttt ccaaagggac atgggaaagc tattcaaata tagacaaatt
     2221 gaacagagta ggacaagcca atgcgatgtt gggaaaagac ttgtttccta ctgaaaagag
     2281 agaacctttg tatattgatc cgacaggttg gaaacaaaag aaattaagtg acggacaatg
     2341 gttatacaac cgcagccatt tgattggttt ccagttgact ggtcaaaata ataacattaa
     2401 aaacttaatg acagggacac gttcgttgaa tgctccgtac atgcttgccc acgaaaatga
     2461 tattgcggca tatatcaagg aaacaaatca tcatgtacgc tatcgtgtca ctcctcattt
     2521 cgagggaaat gaactggtag caagaggtgt gcagttggaa gcagaaagca ttgaagacaa
     2581 aaaaattgaa ttcaatgtct ttatctacaa tgttcaagat ggctacacca tcaattatga
     2641 gacagggcaa gcaacgaaaa cgaaaaatta gtagcagcaa ttcgaatttt ttatgaactt
     2701 tatgaaaaaa ttgtgtaaaa acatactttc aaagaaatat agcgtatact ttatggaaag
     2761 agggcttttg aaaaagccac agaaaaaagg aaaaatcaga caaatcgtgt ttcttaattt
     2821 attcagaagt ttggaatatt tgtttgtagt tttccctttc ttcgagtaaa ataagggtgt
     2881 agaaggtaag ctggaattac cttgcgggaa agaggtgtag aatcatgggt tttacagatg
     2941 aaaccgtacg ttttggtttt gacgatagtc gcaaaaaaga agtgagcgaa acattagcga
     3001 ttgtttatcg ggctttggaa gaaaaaggct acaatccaat caatcagatt gtcggctact
     3061 tgctttctgg agacccggcc tacattcctc gttatcgtga tgcacgaaat ttgattcgcc
     3121 gtcacgaacg cgatgaaatc atggaagtaa ttgtcaaaga ctacctctca aatcatggag
     3181 tgaatttatg agagtaatgg gcttggatgt cggttctaag acagtaggcg ttgctgtgag
     3241 cgatcccttt ggctggacag ctcaaggagt agaaatcatt cgaatcaatg aggcagaaga
     3301 agagtttggt tttgatcggt tgaaggaatt agtggcgcag tacgaagtag accgttttgt
     3361 agttggatta cctaagaata tgaacaattc catcggacca agggcagaag cttccatgtc
     3421 atatggagag aagatcaaag aactattctc cttgccggtt gattatcaag atgaacgact
     3481 aaccactgtt caggcagaac gtatgctagt agagcaggca aatacttcaa gagcgaaacg
     3541 aaaaaaagtc atcgataaat tagcagctgt gatgatttta caaaattatt tagacggctc
     3601 aggtaagcaa ttattttaat aataaaaaga aaagaggtaa tcttatgaca gaacataacc
     3661 attcccacga acacgaagga cacgaacaca tcacattggt ggatgaccaa ggaaatgaaa
     3721 cattatacga aatcttgcta acagttgacg gacaagaaga atttggacgt aactatgttc
     3781 ttttatatcc agctggtgtt tcagaagatg aagatgtaga attacaagct tatgcataca
     3841 tcgaaaacga agatggaaca gaaggcgagt tagaacaaat cgaaacagac gcagaatggg
     3901 atatgatcga agaagtattc aatacattca tggcggaaga agaagaataa gctttaaatg
     3961 gtagctgaac ccttggtaga taagggttca gcttttttgt attttgaatt gaatacgaga
     4021 ttgaatacgt tccgctagaa atttaggtgc tctgcaagct tttcaacggt tttttctttg
     4081 gtttctttag ttagatgagc ataggtatcc attgttaatt tgattgatga gtgtctaagg
     4141 cgttcttgaa cttccttcat ggagactcct gactctaaca gcatactagc atgagtgtgt
     4201 ctggttacat ggttacctag aaatggaata ccagaattct tgcagattgt tatcatacga
     4261 gaataaatag cgtgtttagt aatgatttcc ttgtagattc cacaaaaaat cattgttgga
     4321 tcagtaatac caagggttaa catatatctt ttttgattga tttgccattt ctttagccaa
     4381 ttaatggttt tattgtctaa agatagagaa ccatagctat ttctcgtttt tggacttgaa
     4441 attttgtacc catattttgt ttggcttaag gttttgttta tagtgacggt gtttgaatca
     4501 aagtcaatat ctgaccaatc taaagctaaa gcttcgctaa ttcgacaacc actaaaaaat
     4561 agaaagcgaa gtagcgtttt atcgtagtcg gctctatagg tgcttctttt tgattccaag
     4621 tattggaaga gactttctag ttgatcccgt gaataaattt tgacagtatc tttctttttt
     4681 tcaacgtttc tattgggcat gattacaagt tcacaggggt ttttagtaat tatccctaaa
     4741 gtcattgcat atttgaaaat acggcttgca gtagaatgta ggactttata ttgatctgtt
     4801 gttttagccc actgattcaa tatcttctga acatgttttg tagtaatttc tttaattcta
     4861 aactgcccat attttggaag aatccattta ttaaacttaa tctcaatttt taattgagtg
     4921 acttccttta cagtagtttt atagctctca taccagagat catacacttc tttgaaagtt
     4981 tcgtttgttt cctttttaag tccgttctct tcaaagtcta caagtagtct attaagctcg
     5041 atttgagctt ctttttttgt tttaaaattt ctacgtgtag tacggacttc tttaccagtt
     5101 aatggactga ttccaaggta ggtctgaaat agccaagctt tggttccgtc tttcttttca
     5161 tattgcttaa ttgttgccat tgtttttcct cctatcagtt gcgtggcgtg cttttgatag
     5221 aaaaaatgaa ttaaaaatta ttgtggtttt ttgaaagaat ctgtgcggta ttgcttcctt
     5281 tttggaatgg aatgccaagc gatttacaaa tggtatatat aagtttttcg gtatcttcag
     5341 tgtttacagt catggcagaa aaatacgcat ctattgactc atatggatta tttaatttat
     5401 aagaatcgtc aaatttctca agtaatgcaa ttccatattt ataaattaaa tccgtatcgc
     5461 cgtatttata tccgctgtta gatactcctt catgaaagga ggataaaaac ctatttttaa
     5521 acttgtcaga ggagatggaa tgtctcttat ttataaagtc ctccaaatcc agttcaataa
     5581 tgtctagaca gtaatca
//
LOCUS       DAHUVV010000132         5481 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.135, whole genome shotgun sequence.
ACCESSION   DAHUVV010000132 DAHUVV010000000
VERSION     DAHUVV010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.135"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..288)
                     /locus_tag="NK767_002631"
     CDS             complement(<1..288)
                     /locus_tag="NK767_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695570.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGY"
     gene            complement(586..1560)
                     /gene="sgrA"
                     /locus_tag="NK767_002632"
     CDS             complement(586..1560)
                     /gene="sgrA"
                     /locus_tag="NK767_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HCH6695571.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1756..2595)
                     /locus_tag="NK767_002633"
     CDS             complement(1756..2595)
                     /locus_tag="NK767_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCH6695572.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNRTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2780..3667
                     /locus_tag="NK767_002634"
     CDS             2780..3667
                     /locus_tag="NK767_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCH6695573.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEGAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(4261..4956)
                     /locus_tag="NK767_002635"
     CDS             complement(4261..4956)
                     /locus_tag="NK767_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCH6695574.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(4940..5338)
                     /locus_tag="NK767_002636"
     CDS             complement(4940..5338)
                     /locus_tag="NK767_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCH6695575.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1745 a         1097 c          805 g         1834 t
ORIGIN      
        1 ataaccaatc gttccctcta aaagtataga ataaacttta ctcagtaaaa actgagaaga
       61 aaactcgact tttatcatct tgttttctga aagaaaagat aatttatatt ctggatacct
      121 ttcgcaaaac gattctgctt gttgaatcac cgaattcatc ttagccaggg tgtagtttag
      181 atctaaagcg atatccttca ccacatataa tttctcgtct ctttcataac aatagttcaa
      241 aaattgatat acttgccttt cattgatatc aataaattct tcaaacaaag tacatactcc
      301 tttttatcta ttatttagac ttattccgac agctacatga ataacgtaca aatagattcg
      361 ttactgattt ttcagagata ctaccatgcg cagtgtgcat tgtcaattat tcttgctctt
      421 attttccatt tgtgttcctg ttatttcctg tttctcaaat atgttagtaa acaaaaatag
      481 atctggcaaa gctacaactt cgccaggcaa tacagcactc tcgaatgaaa tattcactca
      541 aaaatctatc gttttattca attttagatc tgttgctttt attctttatt tcactcgatg
      601 gaagagagaa cgaaaatagc ttgctcctac aatcaaaatg agtaacacac ccatccatgt
      661 aattagcttg ttcaaggttc tactaccagt ttctggtaaa tcgctttttc tattatctgt
      721 ttctttcgct ttttcttgat gatcaattcc acgagaaaca aaatcaggct gttttagcac
      781 actttttagg ttgctccctg catttctctt ttgttcctta gttggtatga taggtaccaa
      841 atcaagttca ttatttactt ttcctgtgct gctttcgctg ctcgtgcttc ctgtgctact
      901 ctcactactc atgcttcctg tgctactttc actactcgta ctctccgtac tgctttcgct
      961 gctcgtgctt cctgtgctac tttcactact cgtactctcc gtactgcttt cgctgctcgt
     1021 gttttctgtg ctactttcac tgctcgtact ctctgtactg ctttcaccgc tcgtgttttc
     1081 tgtactgctt tcactacttg tgctatccgt actgcttggc ggtccaacaa ctgtccaact
     1141 gcttggtttc aaattctccg tgatcgtcat ctgtccatca taaaaaacaa tcaaatcaac
     1201 tttatcttta ctcttgatct catcaatcga tagttctgca gaaatcatta ttgtatactc
     1261 aataccagcg gtccattgta cgtcttttaa ataaacttcc ccaataactt tttctgaact
     1321 tccattacca tcccaaccat atgttagaac agcatgtaca tcactagcat tcacgctttt
     1381 gtttggtttg actggaatag taatgacttg ttgtgtcatg tcataaacaa tccgatcagt
     1441 tttcacagta aatgaaagat tctcatttgc ccgttcattt gcagaagtac tgattccaat
     1501 cattgctgca attatactca gcccaagaat aacaaataat acggtcgctg ttttcttcat
     1561 ttctatcact ctccatataa ttaccttcta tttgataaag gaattatata aaatgaaaat
     1621 gatgagaaca cgacagtcag ttgtgaaaat ctaacaaaag atgtcgtgta ttcgcttgaa
     1681 tttgtttact ttttcgttta atcaatgaca gacttaaaaa agcaagaccg atatcagcct
     1741 tgctttcttc atcttttatt gataaacagc agtgtatttc ttcatccgct ttgttttata
     1801 tgcatagagt aacaaaccaa caatcaagac taacaagaat aatgtagttg atatagatgc
     1861 ttcagcccca aagtttcccc cagtaattat tgtattttgc gattggatca gagtggagaa
     1921 aaaaccttgt tcttgcgttc cgctgactgg tatacccaaa atagctcctt gtgtccgatt
     1981 ccaagtaata tgagcaccaa tagccgtaaa taaacttcct gtactgtaga taagtgcgct
     2041 gaaaaagcaa cctgctaaaa agatattgaa aactgccaga aatgtcatgt taggattcaa
     2101 tccgtgcaaa agagcaaata atgcagcaga tccaatcatt gctattaacg gaccaaatct
     2161 aagcatctga tgctgaaaaa agccacggaa taaaagctct tctgatgccc ccactgcaat
     2221 aaataaaata aaactcgtta tcagcggcac aatgtttgcg tttcctgaat ctaaccgata
     2281 cgcccccgtc aaatataatg gactagtaat aagcagaaac actccaattc caacaacgag
     2341 acctcccgct aaataccccc agtcttttcc tcgaacagaa acaccataat cttctattct
     2401 ttttccttcg accttactag caagaaataa agctagtaaa aaaacagcta aatgtccgac
     2461 cgtaccggca attgctccaa tccattcact atttccaata aacatatccg caagtgcatt
     2521 attgacaaga attcctaaaa caaaaccaac taagactatt aaaacagatc caatcaaccg
     2581 aataatcctg atcatctttc atccctcctt tatattaagt ttaacaaccc ttttctttct
     2641 tgtctcgtga aaactatgta aatgttctcc atcatagaga gaatagattt ttacgaacta
     2701 agaatatcta cagcaacctt acgaaaatga aagactgtct ccctaattct tctgttagac
     2761 tgtttttaat aaggaaagga tgggaaattt tttgaaaaaa caactactaa ttatgtgcct
     2821 ttcagcatgt gcaattttac tattctcagg ttgtagcaag tccaatgaaa cgattgcaac
     2881 gtttaaaggt ggaacactta cagttcagga tttttatgat aacccaaaag ttaaatcaaa
     2941 taatcaaaca ctattaaaaa aaatgattgt cttcaaagca tttgatgatg tatacggaaa
     3001 agagatttct tcaaaggaag taacaaaaga atataatgaa caaatcaaaa aactcggtcc
     3061 aaactacaag gaacaattaa aagcagttgg ccaaacagaa gagacatata agaaactctt
     3121 taaacagatg ctggctttcc aatacggttt gaaagccaat gtaaaattaa cagataaaga
     3181 tctagacact gcttggaaag aattctatcc agaagttagt acacagatca ttctattttc
     3241 gactaaagaa ggagcagata aagcaaaaaa agaagcaaat gaaggagaaa atttctccaa
     3301 gcttgttcaa gcttatggaa aaaataaaac attaaaggaa actgatggaa aaatgaattt
     3361 tgattcaacc aatccagaga ttcctactga agtaaaaaaa gcagcattta aactaaaaaa
     3421 cggggaagtt tctgatatca ttcctgtaac agatccaacg acttatcagc aaagctatta
     3481 tctagtaaaa atggtaaaaa aacaggataa aggttctaat aaagacaaat acaaatctga
     3541 gctagaaaaa attgctaccg aagcaaaaac tcttgatacg gaattcatgg ataaaacaat
     3601 ccgtaaagtg atgaaaaaag acaatgtaac tattaaagat ccttatgtaa aaaatatatt
     3661 caaataaaca aatttgaccc aattggggat gtggcaaaat ttgagaagtg atttttggaa
     3721 attccttctt atttttgagg caaaccactt ttgccacaac ttcatcgggg ccttttttta
     3781 tttgtaccaa aacaaatcac tggatttgtt acaatcatga ggatataatt taaaaaagtt
     3841 ttttatttct atcacatgaa tcaatgcgcc attaagtaga ttctttcatg cttccctatt
     3901 ttttcacttg ccgtatcgct tcaaaaacaa ctatcataaa ctcaaactcg actgaaaagt
     3961 tattttaaaa aagactaaaa atatcccttc tggacttgtt cttcttattc tgttatacta
     4021 gagaaacacg aaagatattt ttatgcgaca cttttcattt gtttttatga aatttattca
     4081 aaactggcgt aacttcagag ttacaaggat gtattagagg ttagggaata catcgtttaa
     4141 atgagaaata agtaatagtt agtaatagtc acccaaactt taaattaatg aatattgaat
     4201 cgtcagatag cctcctcgag ttttcgagaa ggctatcgtt tattttatgg agaaattgat
     4261 tcagaagtaa tcagcgacct tttttagttc accaatcgtt tctgcttgga aagtttgcac
     4321 ccaatcttct tggaagcccc tcactgcttt atcaatcgag ggcatctcta atacttgatc
     4381 tagtagctct ttacctaacg ttatcgcatg agctccagat cgaatggctg tagtgacttg
     4441 ttcacaattt ttgaaactag ctgctaatat ttttgttggt ctttgcgtac gttcgatctc
     4501 agtccgcaac gaatcaatca ctttcatagc atcaatgcct tgattttgca tcctgttata
     4561 ataaggtgca atataatctg ctccattatt aatagctaaa tacccttgta attctgtgta
     4621 aatagctgta ccggtaatca aataaccttc ttttttcaac tttttgatcg cttgaaggcc
     4681 tgcttgattc accggcacct tgataaacgt atcttttccc agttcttcta tgataactcg
     4741 agcatcctct atcatttctt cttccgttct tcctattgtc tgaatatgga taggaatctg
     4801 tccgattatt tttttgattt ctttcatatg cgagaagaag tttgacgctc cttctttttt
     4861 cagaattgta ggatttgttg ttacgcctgt taaattcaaa aactgttgac aagaataaat
     4921 actttttgta tttgctgtat caagcatgat ttccatatag ctttccccca ttctgatcct
     4981 cttgatcgat tttaatgatt gtcccctttc ttaaatgcgg catttcctta tcttctacat
     5041 acattgttcc tggaagttcc ggctcatgag atccatcaaa ttttatcgtg atatgtccta
     5101 attcattcaa attagtttgg acaacatctc ctactgctgt gattacatac atttgtttat
     5161 caaaacagag cttcatatca caagttattt cttttcttac ctgattcaat tcaatcttat
     5221 aacaataatc tgttaatgtt tccggtgctt catctccaaa taaaatcatt attttttctt
     5281 tcctgaatac ttctgcttct ataccaacat ctgttacaac cgtttcaaag atcgtcatct
     5341 ctgttcctcc tcgtaaaata tcatttgtct aatagttttt attcaaaatg tttctagatt
     5401 ataaaattta ttgaataaca cattcctaac ggaatttgtt tcgatattgt gtgatttctc
     5461 agagtacaat ggatgtatgg a
//
LOCUS       DAHUVV010000133         5270 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.136, whole genome shotgun sequence.
ACCESSION   DAHUVV010000133 DAHUVV010000000
VERSION     DAHUVV010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.136"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..723
                     /locus_tag="NK767_002637"
     CDS             <1..723
                     /locus_tag="NK767_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HCH6695576.1"
                     /translation="DFLSTPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGL
                     PVLLSTGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPV
                     GYSDHSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTE
                     IFLGDGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQ
                     YIGKKVKRPISADSVLLEEDVE"
     gene            720..1868
                     /gene="neuC"
                     /locus_tag="NK767_002638"
     CDS             720..1868
                     /gene="neuC"
                     /locus_tag="NK767_002638"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HCH6695577.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            1841..2518
                     /locus_tag="NK767_002639"
     CDS             1841..2518
                     /locus_tag="NK767_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCH6695578.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            2508..3650
                     /locus_tag="NK767_002640"
     CDS             2508..3650
                     /locus_tag="NK767_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCH6695579.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            3663..4640
                     /locus_tag="NK767_002641"
     CDS             3663..4640
                     /locus_tag="NK767_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCH6695580.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            4640..>5270
                     /locus_tag="NK767_002642"
     CDS             4640..>5270
                     /locus_tag="NK767_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCH6695581.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYIL"
BASE COUNT         1930 a          660 c         1013 g         1667 t
ORIGIN      
        1 gattttttgt ctactccttt tgatgaagaa agtgcagact ttctaaaaca aattggtgta
       61 caaggattta aaattggctc aggagattta acaaatttac cttttcttag aaaaatagat
      121 atgtatgggt tgccagtttt attatctaca ggtatggctg atattcatga ggtcagtgag
      181 gctgtagatt gtttcataga tagtccagtt actattttac attgtacttc aaattatccg
      241 gctagttctg aagatattaa tttattggcg attaacacat taaaagaaat gttcgggata
      301 cccgttgggt actccgatca ttcattggga tatgatgttg gtatttgtgc tgttgcgatg
      361 ggtgctaagg taattgagaa acattttacc ttggataagg aactacctgg tccagatcat
      421 aaagcttctt tagatcctca ggaatttgca gattttgtaa agcatattag aaacactgaa
      481 atatttttag gtgatggtgt taagcgagca atgccttctg aacttagtac aaaagaagta
      541 tcaagaaaaa gcctagtggt cactgagaat ttaaatgtgg gagatattct tacagagaaa
      601 tctcttacaa taaaacgtcc tggaaatgga atagctccta agtttttata tcaatatatt
      661 ggcaaaaaag ttaaaagacc aataagtgca gatagtgttc tgcttgagga agatgttgaa
      721 tgaaaaaaat acttatagta agtggaacac gagctgatta tggaatatat aaacctgttg
      781 ctgagaattt actacaagat aattgggatg tatcatttgt tgtttctgga atgcatttat
      841 cccataaata tggatttact aaagaattga tcttagctga cggatttaga attagtggag
      901 aagttcaaag tttgttttta gagaacaata agggaaacat ggctcggtct gtggcattag
      961 agataaatgg tttcacaaat atatttgaac aaactagccc tgattttgta ttattactag
     1021 gggatagagg agaaatgttg gcagctgcga tagcttgttt gtatcttgga atcaaaacag
     1081 cacatattca cggaggcgaa gtgtctggta ctgtagatga atcaataaga catgccataa
     1141 cgaaaatagc atctgttcat tttgctgcta cggaaaaaag caaagagcgt ttaataaaaa
     1201 tgggggaaga ttcttggaga gtttttcccg tcggagctcc tagaatagat actattttaa
     1261 ataatagatt acctagtttt agagatataa aagaaaaata tcatctaaaa tttgaaaaga
     1321 aaaaatacgt tttgcaagta tttcatccag ttgtaaccga actagaaaat atagaaaaac
     1381 aaattttagc tttgatcaat tcaatggaac aacaatctca accaattatt tgcattttac
     1441 ctaatagtga tgctggtagc gaaattatca gaaaaatgta ccagaaatat ttaaaaacgg
     1501 atattattta tattacgaat ttagagccgg aggaatacct gactatatta aaagagtgca
     1561 aatttatgat cggaaattct tcttctggga taattgaagc tgcatcattc aaaataccag
     1621 taataaattt aggaaccaga caaaatggac gagaacaatc taaaaatatt ataaatgcaa
     1681 atctaaatga aaatgaaatt gaaactgctt tgaataaaat acaagatcct cagtttataa
     1741 aaagcttgaa aaatgttaag aacatctacg gtgacggcaa cagttctaga agaataacaa
     1801 aaacattgaa tcaaatattg aatgatgggg aagatattaa gtggattcaa aaacaaatag
     1861 cttattaaaa tatactagtg atccatacat tattattggg gctggcggac acgcaaaagt
     1921 cattattgaa accttaagat tagaaaataa gttaatatat ggattttttg atgataagca
     1981 aaataaaatg caagatctga aatatcttgg gaaaataaac gaattgaata gctgggcgga
     2041 tagcaaaaaa aaaatctata aatttattgt agctattggt gactgtgtag taagaaaaaa
     2101 gattataaaa aaattgaata tgccaagtga gcaatatgga atagctgttc atccaagtgc
     2161 tatagtggca aaagaaattt caattggtta tggaactgtt atttttgcga atgctgtaat
     2221 taacccagat gctgtaattg gagagcatgc tataataaac acaggtagca ttgttgagca
     2281 tgattgtaga attaataact atgttcattt atctccaggt gtatgcttag cgggtggagt
     2341 acatgtaggt gtaggaactc aaataggaat tggttctcaa tgtatccaga tgaagaaaat
     2401 tggatcttgg tgtatgatag gcgcagggag cactattgtt aaggatattc cctcacattc
     2461 cttagcttat ggcaatccag ctaaaattaa gaaagaagga attaattttg aattctagaa
     2521 ttttattatc aagtcctcat atgggaggaa cagaagaaaa atatgttgct aaggctttta
     2581 aagataattg gatagcgcca ttaggtgaaa atgtggataa atttgaagaa agtattacaa
     2641 aatatactgg ttctaagggc ggagcttctg cgttaagttc aggtacagct gccatacatc
     2701 ttgccttaag actgcttaat gttggaaaaa acgatattgt ctttgctcct tcatttactt
     2761 ttatagctac agtaaatcct attttatatc aaggggctgt accagtgttc attgactcag
     2821 aacctaatac atggaatatg agccctgttg cactagagag cgcattagaa gaagctgaaa
     2881 gaaacaacca attacctaag gcaatcatcg tagtgaattt atatggtcaa atggctgatt
     2941 atgaacgaat tttagctatt gcaaaccaat ttaatgttcc agtgatagaa gatgcagcgg
     3001 aatcgttagg ttctacttat aagggaattt cgtcaggctt atttgggaca attgggatct
     3061 attcatttaa cggtaataag atcatcacaa cctcaggtgg aggagctcta gtgtctagtg
     3121 ataaaaaata tgttgagcat tcaagatttt tagcaacaca agcaagagat caagcaccgt
     3181 attatcaaca tagcgaaata ggttataatt accgtctaag caatatttct gccggtatcg
     3241 gacgtggaca aatggaagta ttgaatgata gagtagctaa acgaagagaa attttcaatc
     3301 gatataagga agctttttct ggaattgata gaattgaaat gatgccagaa gtggatggca
     3361 tggtgccaaa tcgttggttg tctactttaa cgatcaatga ttataattct attgatcaag
     3421 tagttaacgt aataaataaa ctaaatgaaa aaaacattga aacaagagct ttgtggaaac
     3481 cactacattt gcaaccagta tttcaagggg taaaaatgta ttaccatgat aataatctta
     3541 gtgtgtcaga aaagttattt aatagcggct tatgcttacc ttctggaagt aatatgacag
     3601 atagtgaaca agaatttgtt attgatgagt ttaaaaaaat attcaagtag gtgtgatatt
     3661 ttatgaacat aggaattatt ggtttaggtt ccatggggaa aagaagactt agactcattc
     3721 aagaacactt taatgatatt ttactatgtg gtgttgatac taatgaggcg agacgaaagg
     3781 aagttgaaaa ggaatttgga ataccaactt ataatagtct aacagaggca aaaaatttgt
     3841 ttgaaataaa ttcagttttt gtttgtacat cccctataac ccatgaattt attataaaag
     3901 aggctttaaa tttagattgt aatatcttta cggaaatcaa tttattaaat gaatattatg
     3961 atgaaataat cgaagtagct aaaaagaaag atagattact ttatctatct tctacatttt
     4021 tgaagagaaa agaaattcaa tatattaagt ctaaagtgac gcataataaa aaaatttctt
     4081 atcaatacca tgtaggacag tatttacccg actggcatcc ttgggaagat tatcgcaatt
     4141 tttttgtttc aaacaaaaaa actaatgggt gtagagaaat attcgctatt gaattacctt
     4201 ggattataga tacatttggg aaagttaaat cgtttactgt aatgacccag aaattatcca
     4261 atcttgatat tgattattcg gacagtttca ctcttattat tgagcatgaa actggtatag
     4321 ttggaacttt aagtgtagat gttgtttcaa gagtagctaa aagaaattta actataattg
     4381 gtgaaaagat tttaatagaa tgggaaggta acccggattc tctattttca tggaatgaag
     4441 aaacttctag tatgacagat attcaattgt acgatgaggt tatgcatgat aataattatt
     4501 cacatactat tattgaagat gcatatttag aggagattaa agaatttttc acgcttttgg
     4561 aaaagaaaat ggaaccccaa tatacttttg aaaaagataa gtatattatt aacttaatta
     4621 acgacattga ggtaaattaa tgatgaaagt aatttttttt gggttagggt caattggaaa
     4681 gagacatcta aaaaatctaa taagctatgg aaaaaagaat tctattaatt ttgatataac
     4741 agcttatagt agcagtggaa aaaaagagtt tgaagaaata acgtatattc aaaatagaga
     4801 cgatttggat acacattatg atatagcttt tattacaaat ccgactttta agcatctgga
     4861 aacactaaat gtaattgttg aaaaagttga ttacatattt ttagaaaaac ctgctttttc
     4921 tgagagtatt gatcttactc aatataatta tgattacaaa aatatttatg ttgctgctcc
     4981 actgaggtat aaaaatatca tggaagttgt tcgtaatagt attcaaagta aaaaagtata
     5041 ttctgtaaga gctatttgtt ctacttatct tcctgattgg agaaaaggta ctgattatag
     5101 aaataattat tctgccttta aatctatggg gggaggagta gaattagact gtatacatga
     5161 attggattat attataaacc tatttggttt ccctaaatca gtaaaaaaac agtttggtaa
     5221 atattcgaat cttgaaatta acagtaatga tttagcaaca tatatattga
//
LOCUS       DAHUVV010000134         5192 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.137, whole genome shotgun sequence.
ACCESSION   DAHUVV010000134 DAHUVV010000000
VERSION     DAHUVV010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.137"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(44..502)
                     /locus_tag="NK767_002643"
     CDS             complement(44..502)
                     /locus_tag="NK767_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCH6695582.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKLTRV"
     gene            complement(809..1906)
                     /locus_tag="NK767_002644"
     CDS             complement(809..1906)
                     /locus_tag="NK767_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCH6695583.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(2014..4041)
                     /locus_tag="NK767_002645"
     CDS             complement(2014..4041)
                     /locus_tag="NK767_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCH6695584.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4186..4917)
                     /locus_tag="NK767_002646"
     CDS             complement(4186..4917)
                     /locus_tag="NK767_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCH6695585.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1469 a         1049 c          818 g         1856 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcgtcaact
       61 ttctcttttc aaaaaaaatt tgatattttt ttagatttct ttttagtctt tgtaatactt
      121 tataagccac agaataactc acgtgatgct gtttagcaaa attttctact gagatatatt
      181 tttcctgaaa gaaagccaat accattttca tagataaaga atccgttata taacgagata
      241 acagagcttt gctagaacat tttccattaa tttccagaca tatatccaat tcactgacgg
      301 ttactcgcaa atttttctct agatcgaact tttgtaaatc atgtttcagc ttatcgatcg
      361 tttttttcaa gagaaatgtc gatagcgaca actcttcttg aacattcgat attgactctg
      421 ataaatttga gcttgcttct aaatatttaa gtaatgaata acttctgtga tcttctttgc
      481 ttaaaaaaaa ctcataattc attagtcatc agctctttct acgtatatta ctttccatgc
      541 attcaccaac tagctgggga taaaactgat ttcctggatc cgagtcactg ttgtcagcga
      601 ttcttgtttg tataatatac atcgtcctgt ctttttttta acattttgga gagcgtttta
      661 tttggccttt ctcgcgatca aacctgttat tagaattata tcatttacta gtatgcattt
      721 ttattaataa aatggtatat tttatattgt tataaaaaga gcccaaccat cctacaataa
      781 taaaaattta ctatttgtta tcttaacctt acttttcttt ctcttctttt tttctcaaaa
      841 caaacaaaag tatgcataat aaaataaaca ataagaaaag tgaagctact aagatataaa
      901 tccaattatt gtctttttta atcgtaacat ccgtcgtgtt caattccttg gctacttttc
      961 cgtctattgt aaaatctttt tcaaataccc attgattggt atagttgatc gtagtatttc
     1021 ctttttttct cgaaaatttt ccattttcat tttcatttcc taaaacagtc attgtaaggt
     1081 gatattcacc tggggttagc ggttgaccat ttaaagcagt tggaaaagaa aaatttgtat
     1141 tcggcgcaat ctgcaatcct tctttttcct cttgatataa aacttcagaa tgtccttttt
     1201 ttgtaatttt tgtttcaata actacttgat tgatataggt tttttgatca ttttgaacgt
     1261 ttgctaagat gacatttctt gcattgattt gatctggttt aacttcatga agagtaaggt
     1321 tcggagcaac ttcgttcagt gtctgttgca tcaacaaagc aataatatag gtgtattcat
     1381 tctcaatcga taagctttga tccttagttt ctgtttggtc cttttccgtc tggatttctt
     1441 ggaatgtgat cccaccagcc aatacaccat caaatcgctc attcggcatc ctgacatgaa
     1501 aagcaacggt ttggacactt ttaggtttta gcgtcactat ctgtggatag ctcacataat
     1561 ctaccagatc aaagatcagg ctcatgtctt tttcaatctc gttttttccg tattcaacca
     1621 tcacatttga attggtggtt gcgctattca cagaaatatc gattttcact tctttttctg
     1681 tatcatttct caattccgct tttacttcta cttcctgatc cggatcaagt tgaagatcga
     1741 agtaagtttt tctcttgtcg atttgattct ctgaagttat cggcgtcact gaaaaattaa
     1801 atgcactagc tgacactgat atattgttaa aaagtgcccc taaaataact aggagtgtta
     1861 taaataattg ttgttttggt ctcaagaaaa tcgctccttt aatcatatgt aagagaagac
     1921 tataacaaaa tcttgtctct gtgggacaca agagcctatg gtacctgtgc ccttcttgaa
     1981 actcaataat tttgttatat tcttctcttc ctgttaattt cctgggacat ctgcaagtat
     2041 ccaagatagt ttcccctcaa aggttccgat tctttggttt tctacaggaa tatttattct
     2101 gattccacga tgagctgtag aatctaaaag agaggacaaa tcgctttcac ccttttgatc
     2161 actttcgaaa atcgtaacag cggatgtccc tataaccgtt gtattttcag aggtaacaaa
     2221 ggaaagagct tctggaagct caaaatctcc atttgtaaga actttctctt gtttcaattg
     2281 taatttccaa cttccaaaag cccgttcatc tataattttc aacccacctg aaaactgtcc
     2341 gaattcactt aatggtctac tggatatttt atgttttcca aaattgacat attgaggagc
     2401 agttgagaga taaagtttgc cttttctgta ataaaaatag atatcctctg tattttcgga
     2461 gaagatgcct tctgtattcg ctgaatccga gtcaaaaaca tacccttcaa tatcaatagg
     2521 agtttcttta tacttctctc ctattttccc agtgattgtt ttcatttcag caatatcaat
     2581 ctgactgtct ttatctttat aataaacatg gatatctatc tccttcagct ttgcgatctt
     2641 taggtttatt tcattcgatt gctcaatcaa attcccgttt tcaaggtatt caaaatgcat
     2701 agtgagtact ttttcaggaa ttacttcctg caaagttgtc tccaattcga ttattaacgt
     2761 atctccagca ataaaataat ctgtcagatt tagcgtgact cgatttccgg ttatcaagat
     2821 atctttggca tcaatttgat ttccattgat caagatatgc tcgggaatag ttaattcttg
     2881 tgggaggtcg atactagtct gcattttctt atattcaaga aaatccccct cgaccttcaa
     2941 tgtatttctg atagttatct tatcgtttcc tccagcttca gcgatggccc cataatcact
     3001 atcgttgact tttacatcag ctgttgatgt gacattcgat ggaagtcgcg tgacagcgac
     3061 agcattttct tgaaaaaaag tacctgttga agaagtaaac ccccaattta cttctgtaga
     3121 accaaactgc ctaatgagat caggtataat ccgagaagag cgaccgacac cttccaaatc
     3181 ataagtaaga cttctttgct ccgcgtccca tttaattatt aacaagcgcc atgtgccatt
     3241 agaaagaaat gtaggagcta ccgtcacgcc agtatgctct ccagaataat tattattatt
     3301 tggtttaggc gtgacaaaag cgacatgtcc atgccctcta ttatcagcag ttatctctcg
     3361 atccattcga tcagaagaac cactattttt atacgtatca aactcaatcg agagcgcatt
     3421 cgcaatgtaa ggttggccag aacgagtaga atatgagcca attcccatac ctggcgatcc
     3481 aataacttga ctgggattac tagccattct aggatcgcca gtcaatgtaa aggtcatgcc
     3541 atctcccgct ctatctcgtt gatttcctaa gtataaataa gatttaaatg taaaatccga
     3601 gagtaaacta atcttcttat ttgaccaaat agctcctctt tgagaccttt ctgctgggtt
     3661 caactgcagt aacttcccat tttccaatat ggacgtgcta gtaccgatag gtgtttgaaa
     3721 aattttatcg atagaaataa tattgggatc gtcaggcagc tcaaccgcag aaattggtgc
     3781 atcttctagt ccttgtttgt ccgaaccctc agcttctacg ttgtcagtgg tctctccact
     3841 actcgaaata cttaaatcct cactgctttc ttgtgtctgg ttggattcta ttaaactgtc
     3901 gaatgtcgaa gtgagtttgc tttcttcgtc tcttctattt ccatgctcca actgattcaa
     3961 ttccaaagag taccctatga tcggaaagat attagtcact aagagactgc ttaaaatgag
     4021 cacgatcctc ctctttttca atatatcttt tcctcctgat cgttaaattc ctagatgaac
     4081 tattcgtatc caaacaaatt tagcctagcc tttgacaatt tttgctcgat tacctcgcaa
     4141 gacaatatga ctacgtattt atttttaaca gttcttttca gtaaactagt tgttaggtgt
     4201 atcttctaat gtccaaacta atttagtgga atattgttgc gctaacttga cagatttacc
     4261 gggtaccgaa agctgtaccg catcttgatt ttcattttca tttgcaccaa atcgatagac
     4321 ccaagtcccc acacctgttc ctgatttagc attaattgct aacgtattgt ttcctcccgg
     4381 tgtcaacgtt aattcagaat tcaccgtttg cggcgtatat gttggatcaa ccaaagaaac
     4441 agcctgccct ttcgtaaatt tcagctgtgc tccaactagt tcatccccac tggcagtttt
     4501 aaactgttct ggttgagaga cacttaattt ccacccagat aaggtacttc tcctatctgt
     4561 cacttggaca tatgtcggtt ttctagaccc atcgttcatc acatcagcag ctgcataata
     4621 cgttttatcc gcagtagaaa ttttttgagt cccaaatttg aattttgatc cataatcaat
     4681 cgataatgcc cctcctgttc cagggcttgg aggatctgat ggatcgacag gtttattggg
     4741 atcaggattc tctggatcaa caggaggagt tacaccctca tctcctgctt caaatgtaat
     4801 cgttccttct gtgttatatt cctttgggta tacttcagtt gcagaaacaa aagtcgtact
     4861 tgctaccatg ctgaataaca tcattgctgc tgttaatacg atttcttttt tcatcattga
     4921 cattcctctt tctttattta ctaaacatat cagtcgattg gattaatcca tagcaaaatt
     4981 ctgttttacg atacttatga aataaaagaa catagaccta tatttggtcc tatgtatcga
     5041 ttagttataa atgattatca gcccgatttg atttctagtt tttattgcaa cttgatgcaa
     5101 acacaatcta gaaatacagc tgtcatagcc tttaaaataa ttcgtaagtc cccctcctat
     5161 ctatcaaaat gtgacttgac gagagtgtaa aa
//
LOCUS       DAHUVV010000135         5191 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.138, whole genome shotgun sequence.
ACCESSION   DAHUVV010000135 DAHUVV010000000
VERSION     DAHUVV010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.138"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            137..748
                     /locus_tag="NK767_002647"
     CDS             137..748
                     /locus_tag="NK767_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCH6695586.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="NK767_002648"
     CDS             821..1750
                     /locus_tag="NK767_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCH6695587.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1819..2645
                     /locus_tag="NK767_002649"
                     /pseudo
     CDS             1819..2645
                     /locus_tag="NK767_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            complement(2693..3871)
                     /locus_tag="NK767_002650"
     CDS             complement(2693..3871)
                     /locus_tag="NK767_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCH6695588.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3969..4067
                     /locus_tag="NK767_002651"
     CDS             3969..4067
                     /locus_tag="NK767_002651"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6695589.1"
                     /translation="MKERGLSGVKMVISDAHIGLTKAVKEYLLCAS"
     gene            4098..4499
                     /locus_tag="NK767_002652"
     CDS             4098..4499
                     /locus_tag="NK767_002652"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6695590.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4706..>5191)
                     /locus_tag="NK767_002653"
     CDS             complement(4706..>5191)
                     /locus_tag="NK767_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCH6695591.1"
                     /translation="LKEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKE
                     QQVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFS
                     NNQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVI
                     K"
BASE COUNT         1641 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttta a
//
LOCUS       DAHUVV010000136         5123 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.139, whole genome shotgun sequence.
ACCESSION   DAHUVV010000136 DAHUVV010000000
VERSION     DAHUVV010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.139"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            107..1248
                     /locus_tag="NK767_002654"
     CDS             join(107..335,335..1248)
                     /locus_tag="NK767_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCH6695592.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="NK767_002655"
     CDS             1483..1935
                     /locus_tag="NK767_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695593.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="NK767_002656"
     CDS             1949..2638
                     /locus_tag="NK767_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695594.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="NK767_002657"
     CDS             2763..5114
                     /locus_tag="NK767_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HCH6695595.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1743 a          781 c         1047 g         1552 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg ttt
//
LOCUS       DAHUVV010000137         5004 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.140, whole genome shotgun sequence.
ACCESSION   DAHUVV010000137 DAHUVV010000000
VERSION     DAHUVV010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.140"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..104
                     /locus_tag="NK767_002658"
     CDS             <1..104
                     /locus_tag="NK767_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HCH6695596.1"
                     /translation="LLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            792..1613
                     /locus_tag="NK767_002659"
                     /pseudo
     CDS             792..1613
                     /locus_tag="NK767_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
     gene            1756..1980
                     /locus_tag="NK767_002660"
     CDS             1756..1980
                     /locus_tag="NK767_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6695597.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            1995..2864
                     /locus_tag="NK767_002661"
     CDS             1995..2864
                     /locus_tag="NK767_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCH6695598.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2845..3579
                     /locus_tag="NK767_002662"
     CDS             2845..3579
                     /locus_tag="NK767_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCH6695599.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            3612..4520
                     /locus_tag="NK767_002663"
     CDS             3612..4520
                     /locus_tag="NK767_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HCH6695600.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4517..4765
                     /locus_tag="NK767_002664"
     CDS             4517..4765
                     /locus_tag="NK767_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HCH6695601.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
     gene            complement(4768..>5004)
                     /locus_tag="NK767_002665"
     CDS             complement(4768..>5004)
                     /locus_tag="NK767_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCH6695602.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFGGTLRYSLGY"
BASE COUNT         1690 a          757 c         1084 g         1473 t
ORIGIN      
        1 ctttgctatt agttatggat ttgcaaagtc accttattga acaatacaac ttaaaagtta
       61 cacatgacat tttagaaaga ttgttgaatt acattagtga ataggagagt aacgttattc
      121 acagtattgg gtactgaaaa ttaacagcat acctcagttt ttcaaaaaac agcgaagcct
      181 atggatatgt tcccgtctac cgatagtgca aaaagtgtcg tggatatgtt tccgagaacg
      241 gacatacaca aatgacattc attctgtcct ctcaatccat gtggaagcag tatgtctgtt
      301 aacaagaaaa taattattgt tcgtcctgac gagttgttag actcgaaaag gataaacatc
      361 atttttagaa ccggtagccg atttttggct agcggttttt tgggctcttt ggcaaatagt
      421 ggtgattgaa tctcgcgcta tcctatggaa aaccgccttg tgtgaggggc tgcgagaccg
      481 cttcgatcct agaattgctg aagcacgaat ctagcgtcac tcacatttca tcttttgacg
      541 tagctgagac aaggtgtttt tcttcgatcc gctagtggtt aatctctttt tggtgcaaat
      601 atggtttaga atttctttgc atggcttaag ggagtggaga atcggatatt agtaggattt
      661 acatccacag ataaaaatag gcttgatttt aacagcgaaa ctaaaatacc ctacttaaat
      721 gaagtgatag attagatgtt gggttgttag gagatgctta aaaatcctat gcaatcaata
      781 gtttttagtg tttgttgact tgatccctaa atcgcctgag tactaaacgt tgagtgtgtc
      841 tgcttactgt cactagccag taagtgagac tgtagttaat ctctcatttt ccccagtatt
      901 atgttgaaaa tagccatgaa gcgattattt ctaaagagtt atttttgcag gctcaagaag
      961 aaatttatcg gagaagtaat atctacacag gagaaggcaa gaacaaaaga atttatagta
     1021 gcaagtacgc tttaagtgcc atcaccttct gtggatattg tggcgatatt tataggagaa
     1081 cctattggaa tattcatggc agaaaagaat ttgtctggcg atgcgtgact agaatcgagc
     1141 agggtcccga ggtatgtaag aaccgaaccg taaaagaaga tgaactctat ggtgcggtaa
     1201 tgaccgcaat taataagcta cttgctggtg gcaacaatat gataaagaca ctggaagaaa
     1261 atattcatgc tgtgattgga gaaaccacag aataccaaat ttcagagatt aataccctac
     1321 tagatgaaaa acaaaaagaa ctaatcaagc tggccaataa aggtcaagat tatgaatacc
     1381 tggtagatga gatcgatgag atgcgagata agcgtcagac tcttttagta gaagatgcct
     1441 ccctcagtgg cgagaacgag cgcatcaatg agttgattga atttatccgc aagaacaaat
     1501 tccgcacctt agaatatgat gataagcttg taaggaagat aatccagaac gttaaggtct
     1561 atgaagacca cttcgtcata gccttcaaac caggtatcga aatggaaata tgaaaaccag
     1621 aagtaaatag cccatgactc tgaagtagag ttgtgggttt tcttttaatt gctgattata
     1681 ataatatttt aatgactatt gcatttatca accaaatggt ttatgttatt cttagcataa
     1741 acttggaggt gctgtatgac tacggcagaa atgattaaag aactgtgtga gcaaatgaat
     1801 ataagtgttt ccgaacttgc tagacgtatt ggccagactc cacagaattt taataaaaaa
     1861 ttacaacgag aaacagtaac cttggatgag ttgaaggcca ttgctgatgt gctaggtgtc
     1921 aagtttgtgc aggcatttat tttaccagat ggagatgaaa taaaaatatc taacgaataa
     1981 aggaggatgt ttatatggta ggtaatgtta ttcaaatagt aacagagaaa ttatcatctt
     2041 tgccttttat agaaggcatt gtattagggg gctcacgtgc aagaagcacc catacagaaa
     2101 attctgatat agatatcgga atctattaca attcagactc atttgacctg acagcgatta
     2161 atcaaattgc tacagagttg gatgatgaga atagaaacaa ccttgttgta cctcccggag
     2221 cgtggggtga ttgggttaat ggcggtggat ggttagttat aaacgggtgt catgttgact
     2281 tgatattacg tgatataaaa cgggtggaac aaataatcaa agatacggag caaggaattg
     2341 ttactgccaa ttatcagacc gggcatcccc atggttatat aagtgctatg tatcgtggag
     2401 aattagcgat tagtaaaata ctatatgcta agaacgaaag cttatgcgaa ttaaaaaagc
     2461 aggcagaaat ttaccctact gctctaaaga aaagcttgat gaactttttt atatttgaag
     2521 ctgaattctc tttaatgttt gtaaaagcaa atgcgggggc agaggataaa tattatattg
     2581 caggccatgt ttttcgtatt atttcatgct taaatcaagt actatttgca tgtaataatg
     2641 cttattgcat taacgaaaag aaagctataa aactgcttga aacttttgaa tataaacctg
     2701 aaaaatatgc cgaaagagtt aatcatattt ttgaagtact cggtctttct ctttttgaat
     2761 gctacgacat gaccgagaaa ctttataaag aagtgaatga aattgtatcg gagataaata
     2821 actttttaaa cgaggagagt tcagatgaaa gaaaacaaat atgatgataa tatatttttt
     2881 caaaaataca gtcaaatgag tcgctcgcag aaaggactgg ctggtgcggg agaatgggag
     2941 actttgaaaa agatgctacc tgattttaag ggtaagcgtg tgcttgattt aggatgcggc
     3001 tatggatggc actgtatata tgcgatggaa aacggtgctt cctctgtagt aggtgttgat
     3061 atttctcata aaatgctcga agtagcaaaa ggaaaaaccc attttccaca gattgaatat
     3121 gaatgctgtg ccatagaaga tgtggatttc ccagaggaga gctttgatgt aatactaagt
     3181 tcgcttgcgt ttcattatgt agcagactat gagaatttaa taaaaaagat atataggatg
     3241 ctgaaggctg gtggcaattt agtttttaca gttgaacatc ctgtttttac tgctcatgga
     3301 acacaagact ggtattataa cgaaaaagga gaaatactgc atttcccggt ggacaattat
     3361 tattatgagg gcaaacggac agctatgttt ttggaagaaa aggttacaaa atatcataga
     3421 acactgacca catatctaaa tacactgctt tcaaatagtt ttataataaa tcagattgtg
     3481 gagccacagc cgccagagaa catgatggat attccgggga tggcggatga aatgcgacgc
     3541 ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat aatatagaaa aaataaacga
     3601 ggagtatgta aatgagatca gaaaaagaaa tgatggattt agtactttct ttagcagaac
     3661 aggatgaacg tattcgaatt gtgacccttg aggggtcacg cgcaaatatt aatataccta
     3721 aagatgaatt tcaggattat gatattacat attttgtaag tgatatagaa ccgtttatat
     3781 ctaatgatga ctggcttaat caatttggga atataataat gatgcaaaag ccggaggata
     3841 tggaattatt cccacctgaa gaaaagggat tttcctatct tatgctattt gatgattaca
     3901 ataaaattga tcttacctta ttgcccttgg aagagttaga taattaccta aagggcgata
     3961 aattaataaa ggttctaatt gataaagatt gtagaattaa aagggacata gttccgactg
     4021 atatagatta tcatgtaaga aagccaagcg caagggagta tgatgattgc tgcaatgaat
     4081 tttggaatgt aacaccttat gttattaaag gattgtgccg taaggaaatt ttatttgcta
     4141 ttgatcattt taatcagatt gttcgccatg agctgctgag aatgatatca tggaaggtcg
     4201 gcatcgaaac aggctttaaa ttaagtgtag gcaagaacta taagtttatt gaaaggtata
     4261 tatccgagga tttgtgggag aaacttttgt ccacctaccg gatggattcc tatgaaaaca
     4321 tatgggaagc attatttcta tgccatcaat tgttcagggc ggtatccggt gaggtggcgg
     4381 aaaggcttca ttatgcctat ccggagtatg ataggaatat aacaaaatat accagggaca
     4441 tgtataaaaa atacactggt aaaaccggct gcctggatag cacatatgcc gctgatatag
     4501 aagagaggcg ggaacagtga ttacagaaat gaaagcaggg cacctgaaag atatcgataa
     4561 acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa acgagaattg
     4621 gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag acgaagagga
     4681 tgaagaggat gaggaggcag attgccttga atatattgac aatactgata agataatata
     4741 tcttttaacc cgtggtgcta gttaatttta atatcctaaa gaatatctca aagttcctcc
     4801 aaaacgtcgt tgtgctcgac ttaatagaac actgtaagcc agtaatatgc tttcaaatct
     4861 actctctagc cctttaacag aacgtgagtt gaaattttga caacctaatt tttctaagga
     4921 agaaaaaaca gtctcaactg tttttctttg ttttccaagt aagctgtggt ctagtttttc
     4981 tgatttatcc atgtttttac gtgg
//
LOCUS       DAHUVV010000138         4998 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.141, whole genome shotgun sequence.
ACCESSION   DAHUVV010000138 DAHUVV010000000
VERSION     DAHUVV010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.141"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            213..353
                     /locus_tag="NK767_002666"
     CDS             213..353
                     /locus_tag="NK767_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695603.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            651..908
                     /locus_tag="NK767_002667"
     CDS             651..908
                     /locus_tag="NK767_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HCH6695604.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(879..1187)
                     /locus_tag="NK767_002668"
     CDS             complement(879..1187)
                     /locus_tag="NK767_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695605.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            1265..2038
                     /locus_tag="NK767_002669"
     CDS             1265..2038
                     /locus_tag="NK767_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCH6695606.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIRK
                     HGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSST
                     DSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPKA
                     DGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            2053..2256
                     /locus_tag="NK767_002670"
     CDS             2053..2256
                     /locus_tag="NK767_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6695607.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            2272..2592
                     /locus_tag="NK767_002671"
     CDS             2272..2592
                     /locus_tag="NK767_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCH6695608.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            2597..2767
                     /locus_tag="NK767_002672"
     CDS             2597..2767
                     /locus_tag="NK767_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695609.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPVGRD"
     gene            complement(2774..3556)
                     /locus_tag="NK767_002673"
     CDS             complement(2774..3556)
                     /locus_tag="NK767_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695610.1"
                     /translation="MDISDTPYYQKLIASSKLIGETIAPSLDVVNAMQPAIEQAKKLI
                     AIQDTFIASQAILNDSLIQIVEKRNKSIANMIPPSLFAIQKQLESPSFNIAKKMAATM
                     PKPYFSDISKITERFAVDMSKYDFRIGVATQELEKAFSSEPEVSTKNLLFKDTSNPSD
                     EFEAKFFDILNEQRKVLDDSIKLFEEINTAKNKDITNEPSEKQQASPAFYKDKMWYLE
                     QTGAALIGLVVTEIVNITIGVDPNNTISLALFLRYLLQFLIN"
     gene            3607..4314
                     /locus_tag="NK767_002674"
     CDS             3607..4314
                     /locus_tag="NK767_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HCH6695611.1"
                     /translation="MQELVILKNKEAVTTSLQVAETFRKEHRAVLKAIDDLKDGLAQK
                     YADLFWEDVYIHPQNKQAYRIVYMNRDGFTLAVLGFNNTQKVLEFKLKYIEAFNEMEK
                     AIRKNTAFDTIEDMMIYQLEEMKDVKKDVSMLKNTMRISGQQEFEIKQKGNTKVMEVL
                     GGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRM
                     EIDQLNRQQRLFGDENE"
     gene            4492..4833
                     /locus_tag="NK767_002675"
     CDS             4492..4833
                     /locus_tag="NK767_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695612.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            4826..>4998
                     /locus_tag="NK767_002676"
     CDS             4826..>4998
                     /locus_tag="NK767_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCH6695613.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHY"
BASE COUNT         1872 a          780 c          941 g         1405 t
ORIGIN      
        1 atcattcttg taaacaagtt taacaactaa aaagacaaca aaaaacacta ttgattaata
       61 aatgccaacc gccaagaaag cttttaaaat caatgtttat atgtcttatt taactatgtt
      121 ctgattttaa cactgtatgt taatttagtc aacgcagttt tttaaaaagt tgttaaattt
      181 gtttacgaat ataaaagaaa ggagagaaga atatggaaaa aacagtctcg gcaaaaatcg
      241 aagatttgaa attagacata ctgaaacaag caaaagtggc gatggaacac gcggtagata
      301 aagaagactc tgccatggtt gcagccatag cagagatttt agctcacgtt tagtcatcgt
      361 ttttacggtc ttctggaagc tgaccataga taacagcata atgaatatct aaataagctt
      421 gaacaatttc ttttggcgaa attgattcgc ctttagtaga tacttgtgat tcgtgattta
      481 gccatgcaac aactaaatcc gcagcgattt caaacggcgt tacactttgt gtcagttgcc
      541 ataaagaatt tcacaatatg tatggtcgtg gtaataatac caaagagcaa tttgaggaat
      601 ttatggaaat taaaaaatac aaaaaatcat ttattaaagg aggctaaaca atgaacaagg
      661 aaatgagcca aacaatcaag gtacaaaaaa tgatagatga tctaacacat ggaattgata
      721 gccaagccga taaaatcatc aaggaattgc aaggacaaaa agttaaggat gcaaaaatgc
      781 ttctcaagac tatcaacttt gaaatgaatc caacaaaaag aaagctcgcc gatgtgttgg
      841 aagaaaagtt ggcttccgct atcaacgagc aagaattact atttgaaacc gatactttta
      901 acagctgatg ttttatagat gacagtttca tccgaatcta tatcatcgcc gagagaaaag
      961 aatgttgctt ttgacaaaaa gccgataaga cctacaagcg gtcttgatgt tgcaagagta
     1021 gataccccaa tatcaccttg caaggtattc acgtaaattt gagaagaata gaacattcca
     1081 tctttttctt cgatatttga cgcatcatcg tatccaacga ccaaagtatc ggcaaatatt
     1141 ttgatttttt ctccatcaag catagtaatt acaaattctg gtttcataaa ttttcacctc
     1201 cttaacaatt atttcagcct gtcacactga taaggaaatt ataccaaaga aaggaatgag
     1261 aaatatgaat acaccacaaa tttttaattt cgagcaaaac gaagttcgaa ctattttagt
     1321 aaatgatgaa ccatattttg taggaaaaga tgtagcagac gttctaggat attcgaatcc
     1381 gcaaaaagct attcgagatc atgtggactt ggaggataag acgcagaacg attcgttcac
     1441 cgtcaatgga acagcagttg ttttgattaa cgagtcaggc ctttacagtt taatcttaaa
     1501 atcaaaactt ccctctgcca aaaaatttaa acgttgggta acaagtgaag tgttgccagc
     1561 aattagaaaa catggaggtt atctaactcc agaaaaagta gaagaagctt tgcttaatcc
     1621 agatacaatc attcaattag caactcaact aaaagaagaa agaactggaa gattaatcgc
     1681 agaacaaaag attgcagagt acgaacctaa aatctcctat ttagatagca tattatcttc
     1741 tacagattca gtaacaatta gtcagattgc agcagattat gggatgtctc cacaacagat
     1801 gaataaatta cttcataaac taggtattca gaaaaaagtc ggtaaccaat ggttattgtg
     1861 caaaaaacac atgaaccaag gatacacaaa atctcataca actgagatcc cgaaagccga
     1921 tggtggcact aaaattgtaa tgaataccaa atggacacag aaagggcgtc tatttatcta
     1981 cgaattacta aaaaaagaag gatattaccc tcaaatggat ttagaggaaa ttggttagaa
     2041 aggagtttta gtatgactga cattgcagaa atcactcaac gagatagaga aaaaatcaaa
     2101 gaatatgtcg aaagttcaaa gttcttaact tacaccatgc tcgctgaaag attcggcatt
     2161 agcaaaagct acttatcttt aattttaaac ggtaaaaaga cttctgcaga agcaaataga
     2221 atcatagatt cgattattac tatgtacgaa ttgtaaaggg aggaacagct aatgcaatat
     2281 ctagaagcga aaatcccagt tccagaaggc tatgtaatta tctcccaagt ggattatgag
     2341 gagttaaaaa aagctgatga tactggtaga tggatgacgt tgccagaagt gctagaacgg
     2401 attaacagaa aatatgattg gtttacttct agagttttaa agaacccaag atatagaaat
     2461 attatcgata tcgaaaaaaa caaaaatgga tttgtctatt atccagttga aggaagagac
     2521 acatatctat ttttaagaag taaaacactt gaatttttag aaacaaattt ttcggaaatc
     2581 ttaaggaggt aagcggatgg gtaaatttaa tagagcacta gtattcagcg caccactaat
     2641 tatctacgct ttaggtcttt ggggaagcag acaagcatta atcggcacaa ttgtatatat
     2701 ggtctggatc tttatggggc ttgatgaagc tgaatataga gcgaaaaagc cagtcgggag
     2761 ggactgactt aacctaatta attaaaaatt gtaacaaata acgcaaaaat aaagctaatg
     2821 aaattgtatt gttaggatct actccaatag ttatatttac aatttcggtt actactagtc
     2881 ctatcaaagc tgccccagtt tgctctaaat accacatttt atctttgtag aaagcaggtg
     2941 aagcttgttg tttttcagat ggttcattag ttatatcttt gttcttggct gtatttattt
     3001 cttcaaaaag ctttatagaa tcatcaagga ctttcctttg ctcattaagg atatcaaaaa
     3061 acttagcctc aaactcatca gatggattag aagtgtcttt aaaaagtaga ttttttgtac
     3121 taacttccgg ttcggaagaa aatgcttttt ctaattcttg ggtagctacc cctattctga
     3181 agtcgtactt agacatgtca acggcaaatc tttctgtgat tttagagata tcagaaaaat
     3241 aaggttttgg catagtagct gccatttttt tagcaatatt aaaacttgga gattctaatt
     3301 gtttctgtat tgcgaacaaa gaaggaggaa tcatattagc aatagatttg ttacgtttct
     3361 cgacaatttg aatgagagaa tcattgagta ttgcttgcga agctataaaa gtatcttgaa
     3421 ttgcaattaa ttttttagct tgttctatcg caggctgcat agcgttaacg acatctagag
     3481 aaggagcaat ggtttcaccg attaattttg aagaagctat caatttttga taataaggag
     3541 tatctgaaat atccaattat atacaccacc tttttaccta aattatacca aaagggagag
     3601 aaaaaaatgc aagaattagt aattttgaaa aataaagaag ctgtgactac gagcttacaa
     3661 gtcgcagaaa cattccgaaa ggaacataga gcagttttaa aggcgattga cgatctaaaa
     3721 gatgggcttg cacaaaagta tgcagaccta ttttgggaag atgtgtacat ccatccgcaa
     3781 aacaaacagg cttatcgaat tgtctatatg aatcgtgatg gctttactct tgctgtatta
     3841 ggattcaaca atactcaaaa agttttagag ttcaaattga aatacattga agcgttcaac
     3901 gaaatggaaa aggcaattcg caaaaataca gcttttgaca caattgaaga catgatgatc
     3961 tatcaattag aagaaatgaa agatgttaaa aaagatgttt ccatgcttaa aaatactatg
     4021 cgaattagcg gacaacaaga gtttgaaatt aagcaaaaag gaaatacgaa agttatggaa
     4081 gttctagggg gaaaagaaag ccgagcttat gaagaaatca gcaaaaaagt attctcaaaa
     4141 ttttggtctg aatttaaacg taccttttca atcccaagat atggcgagtt acctcgtaag
     4201 agattcgatg atgctgtttc atttattgaa atgtggttac cagaaactgc gattcgcatg
     4261 gaaatcgatc aactgaacag acaacaaaga ctttttggtg atgaaaatga atagagctga
     4321 agcgctaaga atcgggacgg taattgctaa tcgctggtgg agacacaata aaccaatcat
     4381 cctaagccaa caacatattg acaagcaaaa agcatggcaa caaataaaaa agtgactccg
     4441 ccggcaagca aagagtcaca aagaaaacac atcataagga gattttagca tatggaaaaa
     4501 gaactttcca ctctagatca atatttgact gatcctagtt ggggcaaatc gaatatcaag
     4561 gaaacaaata atcgaaaaat cagacgaaat cttttgacgg atgaagaact agcatgtgat
     4621 caagacgatt tgggaaattt tgtgagtatt tgggatcatg tctatcttat ccatttatcg
     4681 aggaagtcca aaaaacctga atatatctat gtcatcgaag atggcttgat tgatgcgcta
     4741 gaagagtatg acagagataa cttgattgat atctcttatt acggatcagg taagaaatac
     4801 attgctgaaa tggaggcaga atttgatgag tgaaaacaaa gggacaacga cttttgaaaa
     4861 actttttagt cgcaagttaa ataaaatact caagaaaaaa ggaaattttg attatttatc
     4921 ttgggctcac gcatgggaga ttatgaaaaa gaatgatcca caggcaacgg taactattaa
     4981 tgagtataaa cactacag
//
LOCUS       DAHUVV010000139         4954 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.142, whole genome shotgun sequence.
ACCESSION   DAHUVV010000139 DAHUVV010000000
VERSION     DAHUVV010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.142"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..1153)
                     /locus_tag="NK767_002677"
     CDS             complement(<1..1153)
                     /locus_tag="NK767_002677"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCH6695614.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1338..3779
                     /locus_tag="NK767_002678"
     CDS             1338..3779
                     /locus_tag="NK767_002678"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCH6695615.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(4109..4813)
                     /locus_tag="NK767_002679"
     CDS             complement(4109..4813)
                     /locus_tag="NK767_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695616.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1552 a          915 c         1039 g         1448 t
ORIGIN      
        1 atattttgta gtaacgaata tcattttcga tctttgtaat cgaaaaaggt acatctgaag
       61 tatattctgc acattctccg tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt
      121 caacagtgat ccgtgttcga gaatctagca tctcttgatg agattgtgcg aagagatatt
      181 cttgatagtt ttcttccaga taacctgtaa aaaattcact tactgcacca gaaccgtagc
      241 tgaatagacc gatgcgatct cctggctgta gtgatttcga attttccagt agagaagtta
      301 gccccaggta taatgaacca gtgtaaagat taccgattcg tcggctgtaa cggatgcttt
      361 cttcatagcg agccataaga cgttcctgat tttcttcgtc tgtttggtct aatacgcttt
      421 gcaatgcctt ttttcccatc ttagtatacg gaatatggaa agcaatcgcc tgataatctt
      481 cgagtcctcg acccgacaat tctttatgtc gattccaaac tttttggaat gattcgatat
      541 acgtagaatt agataaagga ccatcaacaa caggaaattc gctataatct ggacgccaga
      601 aatcatagat atcttctgtc agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt
      661 tttgagtgat catcatcgca acagcaccga caccttgcgt cacttcacca ccgcttgcca
      721 agccgtaacg agcaatatca cttgctatga ctagtacttt tcgttctgga tgatttttga
      781 catattcttt cgccatgtgc aaagctgcgg tgccaccgta gcaagcctct tttacttcaa
      841 aagaacgagc aaaagattgg attttcagta aatggtggat aattaccgca ctcgcttttg
      901 aatgatcaat cccggattct gttccgacga tgaccatgtc gattagctca cggtcttttt
      961 ctgttacgat tttgctggca gcatttgctc ctagtgtaat gatgtcttca tttgcacgat
     1021 tgactgccat ttggtcttga ccaatcccta tatggtattt tgcaggatca tccccacggc
     1081 tttctgccaa ctctgtcata tctaaatata aattaggaat aaaaaaggaa agacgatcaa
     1141 tccctatttt catttcaaca cctctttact tatgataaaa aacttacttt actaaggtat
     1201 cataaaaaga ttaaaagaaa tataaaaaag agaatattta aggattgtta aaatttaatc
     1261 ttagaaaaaa ataactggaa caagagctat ttgtttttac agcaaagaat ggtatatttt
     1321 tgtaggaggt ggcttacttg aaagaagtcg ttatgataga tgctgcaaga acgccgatag
     1381 gaaagtatcg cggaagttta agtccattta ctgcagttga attaggcaca cttgtgacta
     1441 aaggattatt agataaaaca aaactgaaaa aagataaaat cgatcaagta atatttggaa
     1501 atgtcctgca agctggtaat ggtcaaaatg ttgccagaca gattgctttg aacagcggtc
     1561 tccctgtaga tgtcccagca atgacgatca atgaagtttg cggatctggt atgaaagcag
     1621 tgatcttagc acgccagctg atccaattag gtgaagcaga acttgtgata gccggcggaa
     1681 cagagagtat gtctcaagca cctatgctga aacagtatca gtcagaaaca aatgaatatg
     1741 gtgaaccaat ttccagtatg gtcaacgacg gattgactga cgcattttca aatgcacata
     1801 tgggattaac cgcagagaat gttgcaacac aattttctgt gagcagagaa gagcaggatc
     1861 gctatgcctt gtcgtcccag ttgaaagcag cacatgctgt cgaagccggt gtattttctg
     1921 aggagatcat cccagtcaag atttctgatg aagacgtgtt atctgaggat gaagcagttc
     1981 gtggaaatag tacattggaa aaactgggca cgttacgtac agtattctca gaagaaggaa
     2041 ctgtaacagc aggaaatgct tccccgttga atgacggtgc ctctgtggtg atccttgcat
     2101 ccaaagaata cgcagaaaat aataatctgc cttatttagc aaccatcaaa gaagtagcgg
     2161 aggtcggtat tgacccaagt atcatgggaa tcgctccaat caaagccatt caaaaattga
     2221 cagatagatc aggaatgaat ttatcaacaa tcgatctttt tgagatcaat gaagcatttg
     2281 cagcttcttc tatcgtagtt agccaagaac tgcagttaga tgaagaaaag gtcaatatat
     2341 atggaggagc aatcgcatta ggccatccaa ttggtgcaag cggtgcacgt atcttgacta
     2401 cattagctta cggattgctt cgtgaacaga agagatacgg tattgcttca ttatgtattg
     2461 gtggaggttt aggcttggca gtcttgttag aagcgaatat ggagcaaaca cataaggatg
     2521 tgcaaaaaaa aaagttttat cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa
     2581 aaaatgtatt gacacaagaa actgcgttga tttttcagga acaaacactt tctgaagagc
     2641 tctccgatca catgatcgaa aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc
     2701 agaattttca aatcaatggc aagaaaaaat ggattccgat ggcaacagag gaaccatctg
     2761 tcattgcagc tgcaagtaat ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc
     2821 agcggctgat gcgagggcaa atcgttctat caggaaaatc ggagtatcaa gcagtgatca
     2881 atgcagttaa tcatcgaaaa gaagagctga ttctttgtgc aaatgaaagt tatccttcaa
     2941 tcgtgaaacg cgggggcggt gttcaagata tctctactcg ggaattcatg gggagtttcc
     3001 atgcatactt atctattgat tttttagtgg atgtaaaaga tgcaatgggc gctaacatga
     3061 tcaactcgat tcttgagagt gtggcaaaca agttacggga gtggttccct gaagaagaga
     3121 ttctattcag tattttaagt aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa
     3181 tcccattcga acgcttaggg agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc
     3241 aacaggcagg ggaatatgcg aagcttgacc cttaccgtgc agcaacgcac aacaaaggga
     3301 tcatgaatgg tatagaagct gtagtggcag ctacaggaaa tgatacacgt gcggttagcg
     3361 cttcgatcca cgcctacgcg gcaagaaatg gattatacca aggattaaca gactggcaga
     3421 tcaaagggga taaactagtt ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg
     3481 gagcttccaa tatcctgcca aaagcaaaag catctttagc tatgttagat attgattcag
     3541 ccaaggaact agcacaagta attgctgcag tagggttagc tcaaaacctg gctgctttgc
     3601 gtgcattagt tacagaagga atccaaaaag gccacatggg acttcaagct cgttcattag
     3661 cgatctcaat tggggctatt ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag
     3721 cagaaaagat gaaccagcag acagctatac aaatattgga aaaaatccgg gaaaaataaa
     3781 aaagcaatat ctttagaaga ataaagcaag atttacagtt attttattta ctgcagatct
     3841 tgcttttttc tctatcagaa aatatgttta ttattagaat tttttatagt taaggttagg
     3901 catcttaaga aatcaggcaa ctccaagata tcttcaggta gaagagaagt cgcctaacat
     3961 tgtaggatca taggtgattt gttggacagg tcaagctgat atttgagcct gatgttagaa
     4021 ggaatttctt tttacttgct gtagaaatta ttatatgact aaaaaggctg ggattttaaa
     4081 tcccagcctt ttatgacaaa tagaattttt atcgatcagt aatcgctgtc gctgcaaatt
     4141 gttcgatcaa aggaatcaaa gtcaatttga ctttgtattt ccgttcgagt gctttttcga
     4201 taacgaactc attttcagta gtcatagaaa tagttgctaa tttacgaggt tcatcagcat
     4261 ttttttcatt aataactact tcatgttcat tttgttctgt gtaaaattta ggatactcag
     4321 caacggcttt tgaaaaatca gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat
     4381 tttcagttgt gtccgctttt ggtttttccg ttttttcttc tggcacactg actgtttctt
     4441 ctgtaactgg tgtcgcaaca gcttgcgcag ttggttcatc gtttttcaat cgttgatcat
     4501 tccagtaaga cacgatttca ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt
     4561 ctcctgaacg aattctcttt aatacagtat tgaagttgtc atcgattggc acgttgatcc
     4621 gtgtatcttc atttgttttc ccagctaatc cgatcgttaa gatttgacgg tttttgggat
     4681 caagcatgac ttgtaacggc aaaccataac gtcgcaagct ctcttcttct agagaacgga
     4741 ataattgtcc tcttacacca tccaatgaag gacggaattg atttaataat ttttgtttct
     4801 gttttttctt catatgttct ctccttcgct catacccttt cgattataag aaaaaaacag
     4861 ccaaatgaaa aggagtaatg caaaaaaact tgcgtatacg caaattacaa cagaaaaatt
     4921 tgcgtatttt cccgtttttt gaaagaaaaa taaa
//
LOCUS       DAHUVV010000140         4797 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.143, whole genome shotgun sequence.
ACCESSION   DAHUVV010000140 DAHUVV010000000
VERSION     DAHUVV010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.143"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            63..296
                     /locus_tag="NK767_002680"
     CDS             63..296
                     /locus_tag="NK767_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695617.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(455..577)
                     /locus_tag="NK767_002681"
     CDS             complement(455..577)
                     /locus_tag="NK767_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCH6695618.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            767..991
                     /locus_tag="NK767_002682"
     CDS             767..991
                     /locus_tag="NK767_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695619.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            1434..1640
                     /locus_tag="NK767_002683"
     CDS             1434..1640
                     /locus_tag="NK767_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCH6695620.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            1640..1891
                     /locus_tag="NK767_002684"
     CDS             1640..1891
                     /locus_tag="NK767_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695621.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            1907..2062
                     /locus_tag="NK767_002685"
     CDS             1907..2062
                     /locus_tag="NK767_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695622.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIE"
     gene            2072..2344
                     /locus_tag="NK767_002686"
     CDS             2072..2344
                     /locus_tag="NK767_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695623.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            2789..2968
                     /locus_tag="NK767_002687"
     CDS             2789..2968
                     /locus_tag="NK767_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695624.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            3027..3383
                     /locus_tag="NK767_002688"
     CDS             3027..3383
                     /locus_tag="NK767_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695625.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
     gene            complement(4352..>4797)
                     /locus_tag="NK767_002689"
     CDS             complement(4352..>4797)
                     /locus_tag="NK767_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCH6695626.1"
                     /translation="IHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQSVPK
                     NLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRKDGLPKEM
                     EFNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT         1655 a          750 c          930 g         1462 t
ORIGIN      
        1 gatgatctac cgttttagtt tgagaaagaa ggagtaagca tggcaaaaaa attagtctga
       61 cgatggatct attgataccc gtaattgcat ttatagacgg gatttctttg ggcgtcactg
      121 aaaatccttt ggtaaataga attgcgaaag gatcactatt tcttgtattc gtgtatcttg
      181 tctgttctta tcttttaaaa agagcaggaa aggataattc cttttggcga ttgactaaag
      241 tcacagtagc agcgacagct gttatactta cggctattct accgctttat atgtagagtt
      301 aacgggaaat cgttgaactt cttgcgaaat attgctataa tgaacatgaa aaagaggagt
      361 tgcgctaaca acccctcatt aacagcaccg attaaagaac ggtggcagaa tattagttat
      421 ttggaaataa ccgctgacct gctaaagtag acggttattt ctttttgtca tttttaagca
      481 tatttacaat caaactaatc aaagcaatgg taaacatacc aaaaccaaga atggtttgaa
      541 ttgtctcata tgcggacaaa aggcttactc ctttcataga ttttagcact tacattcata
      601 agcaccacca cctttccaga agtcgccacc gtcttcaaac tttactgtct tgactattat
      661 agcatatcta aaatcgaata aaaattctca attttagctg tttttgctat aattttagcg
      721 aggtgatcgt gatggaacaa tatattggtt tactcgataa attagggtga tcaagacaca
      781 gccattgctt gtgcgattta cattacgcac gagtcatgaa ccaatcaatt gtgtagtggc
      841 caatatagaa atagcggata aattgctgat tttggaagaa ggcaagtata ctatttcagt
      901 gacaggtcac ttcaataaac gaaagcaact agtcattgaa tcaatgcaaa ttagaaaccc
      961 tgatcatttt acgcgatcga tgagcatata aaagaagagc tgcagtccta aatgaggatt
     1021 gtagctcttt ttgttttaca tagaaacagt aaaaatgaaa aggcctgttt aggaaggcac
     1081 aagcgccttc tcaattgaaa gacctttctc tcactccaac cgccaccgcc gttcgagaag
     1141 aaaggaagtg aacgctacgc gttcactaag ataaagggtc ttgcatagca agacaaaata
     1201 gaaaaaattt cctctcacca aaaggttcgc tcctgttcgt tatctcttta ttgatttgtc
     1261 agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg taaatggcac
     1321 gctgcgcgcc cttgtcttgt cgcttttcgt tgattcattc tggccaacaa atcaataaag
     1381 aaagaactaa cgaaaggaag taaaaccttt cctaagagga ggcagaatca atcatgcgaa
     1441 agaaatttga ttattggggt gttcccttca gcgaattatt cccaaactat catgcaccac
     1501 atactgtcga atgcgattgt ggagaaagag cgaaatgtat caaaagctat tgtttgtatc
     1561 aatgtccaac atgcgggaaa aaatatacct tgtcttatgg cgattatatt ttaattgatg
     1621 agaaaggaaa gaaacgctga tgaaaacaca acacaaatta ttgactgtaa aagaagtcgt
     1681 tcaaattttg gtggacaaag aagtcatttc tgaaaaagta gcagaagttt tacttattcc
     1741 gcggtactgg acgatagaaa cccctttagt agaggatgca ccgactatgc tatttgaaga
     1801 cctagtgaag attgcaggat atgaactgca ttatcaagaa aacttggaaa cttatgaaaa
     1861 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaaatatgg ctcaaaatta
     1921 ctatgacgaa tttgtcaaat tacctttgga taaaatggca caaaaaatgg aggatatgac
     1981 gttcctatac aacgaaacac gtgttccaaa aaaacattat aaagaaaaat tgtcggtagc
     2041 tgtggaagaa atgattgaat aaggtgtcga aatgaatctt attgcgacgt actatcgaac
     2101 actggaagaa ctgaaaaagc aaaatgcaaa atggtttttc caagccttac tatgcttgga
     2161 agtaggtgtc aaaccaagca ctattaaacc ttctgaatac caagctttag aactaacgta
     2221 tgcgaaattt atcgaaacga aaaaagccaa gactgtttca agtgaatggt tagattattt
     2281 tgaaaatatc aataagtatg gcgcatatta caccatgaaa aaggaagaca acgaaaatga
     2341 ataaggtgat ttatcaacta acaacggatt ccatatcaac gatttgaaag aaaaaaaggt
     2401 caataatcat ttataccgaa aagtgagaat gtgtgaaaca aggacaaaaa aagcctagta
     2461 actaaggaaa tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg
     2521 tgcgaaataa acgggcaaag cccgataact taagagaaag aaggtgatcc atttgaacaa
     2581 gattattccc ttattgttag tactgtttgt tggcgaaata cttatgcgag atagtgcagc
     2641 tgtacagtta aacctcgctg tatttttggt aatactggtg ttttgtttga tcttttggcg
     2701 gtttgtatta gtcctcgtga gtctactatt caagatgatt gcagccatat ttttcatagg
     2761 gttatttcga tgaaaggagt gagtaccaat gccaattgca ataggaaata aacggttacc
     2821 cgtgacatta gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtatggcaa
     2881 aagtgaatct aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg
     2941 atttgatgta ccagcgctaa aaaagtgacg tcacttttta atttgatcta tataagtgat
     3001 atcactttta ccaaggaggg aattaaatgt taaaaactaa aaagaattcg tacaaattaa
     3061 agcttagtat actatctatt atttgtcttt taggcatgat aattagtggt catttgtata
     3121 gagtggcata tacgcagttt attgagtaca aggttcactc tttaggatta gctggggatc
     3181 gatacgttga atatgtccat tattcattaa aatgttcttc atatattctt ctttggctta
     3241 ttttttcatt gtttgaattg ctaaatttaa tagcaatatc ttcatatcac ttagataagc
     3301 taaataccaa atctagccgt tataacgaag tgtttaagga aaatgtgaag ttagaaagtg
     3361 acttaaacaa gctaaaaaaa taaaaagaag aaaacataag ttaactaaaa ttattttatt
     3421 aagaatgatg gaatataagg ataaggttac caacataacg atcattactt ctctgatatg
     3481 ctatggactt ctatcaatca gtattttgtc tatagattat ggagtaacta tactgcaagc
     3541 cttagttttg gctatgccta tgtatctatc tttgtttatc atgcatgaaa tatataagct
     3601 atttgtttta tcaaaaaatg tagaaatacg ggtaaatatt ttggtgaata gttatcaaaa
     3661 agaactcaaa gaaagaagac ggtggcggta tttattgaaa ggatgaaaaa gtaggatttg
     3721 acatactggt attcagaaag tgccgtcact tttaaaacaa tggaaaaggt ggagataaga
     3781 tgaagaaaaa acaaactaaa ctatcattag cagatattta acacagttaa ccgcaactga
     3841 agatggtgtt gttgaatttg aacaatctga aattgcggta gtagatgatc gttaccttaa
     3901 aatgccttac ttctttgacc aagcaaaggt catttgcctt tgtcactacg atggggtacg
     3961 tgattttagg cgttaaaaag tagtgattat atcaacttta cgagtaaata taaatttaga
     4021 catgaaactg aattatccaa tggaaaatca tggtttataa tctagaaaaa ttatagtaat
     4081 gattggatta tatatattct ttcgtatttt cgtacaactg tagcttgtca aaattgagaa
     4141 atttaaccga ttaggtactt tacatcgtta tttgggtaat ttctaaaaca tgaagcttac
     4201 caacgaataa actgagacta tcttattaat tgatcaatat aagaaaatat ctttgaggag
     4261 atgatgtact atgttttcgt ctatccacga ttggttaggt ggtcttttcg gcggtggagg
     4321 taaaggtttt taataaatat tatttgtcaa attaagcgag acaaaacttt ccatttacgt
     4381 aactcaaaaa aacttctaat ggtgtttggt aatttagtga ttttctaggg atatggtttc
     4441 ttttggacgc aacggatgag atgaatcctt gattgacttg gttgaattcc atttcttttg
     4501 gtagtccatc ttttcggagg agtccgtttg aatgttcatt taatccccgt tgggaaggcg
     4561 ttcctgggtc tgcgaaataa atatcaatat cattcgtatt actaatactt ttccaattag
     4621 agaattcttt tccacaatca aaggtaattg atttgaaaag atttttgggt acggattgaa
     4681 gccattcatt aatcgaattt tcaatatcaa ctgccttacg gccttctggt ttcaaggcga
     4741 taatggcttt tgaaagtcgc tcaacgagtg tgatgacggc acttttatgg tggatgc
//
LOCUS       DAHUVV010000141         4720 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.144, whole genome shotgun sequence.
ACCESSION   DAHUVV010000141 DAHUVV010000000
VERSION     DAHUVV010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.144"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(117..1079)
                     /locus_tag="NK767_002690"
     CDS             complement(117..1079)
                     /locus_tag="NK767_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6695627.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1081..2520)
                     /locus_tag="NK767_002691"
     CDS             complement(1081..2520)
                     /locus_tag="NK767_002691"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCH6695628.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2705..4651
                     /locus_tag="NK767_002692"
     CDS             2705..4651
                     /locus_tag="NK767_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HCH6695629.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1499 a          806 c          776 g         1639 t
ORIGIN      
        1 tctaactttt gggggtcact acaagttgac aactcctttt tgtttacaaa tcaattggat
       61 atctacaaat tgtattttct aacgaatttt atagacatta aaaaattaat ttaacgttaa
      121 atacttcctc cagaaagtat tgagattggt aaaatataat ccttatttgt cttttcgcct
      181 ttaatttttt tcagaagaat atcaacaata agacttgcaa tttcatcaat tggttgtctt
      241 attgttgtca attgtgggaa aaatttctca ataaaagagg taccatcgta tccaataatt
      301 tttaaatctt ctgggatatt aagtttaagt tgatgagcaa ttttcatagt gagaatggct
      361 gttaaatcat cagaaacaaa aataccatct ggtttgtgat ttataatagt tgactttatt
      421 tccatttcac gacggatagt agacagatta ttaggaattt tgaaaatttt tccatttgga
      481 ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag aatccgtgtt atcatttcct
      541 gtaatcataa tggtattttg acaaccattt ttttgtaaag tcttagctgc aagttttccc
      601 ccttcaaagt tatctgaaga aacaatggaa acattaggag ctaaatttct atcaaaagca
      661 acaataggtg ctctcacacg ctcataatca gcaattccaa gattatggct tgatgagatt
      721 atgccatcaa cttggttggc ttcaagcatt tcaatgtatt ctctctcttt agcaggatca
      781 ttttgactat tgcaaataat cgttttatat ccatgtttaa ataactctat ttcgagatac
      841 tcaatgagtt cagagtaaaa gatattacta atattaggaa aaataagacc aataagctga
      901 ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt tataaccaag agttttcata
      961 gcttcatgaa ctttttgttt tgttttttct gataaatatc ccttattatt aatgacgcgt
     1021 gaaacggttg ttgggctaac tccagctaat tcagcgacat cagttaattt tgctaccatt
     1081 ttaatatttt aattcaaagt attttccagt aggttcgcct gatttgattt gaataccatt
     1141 ttggtgattt tcagggaata cacgactagt aaacactttt tctcctttat taataaaaat
     1201 ctcaatgatt gagttatcta caaaaatgtt agcagttgtt tctttagtat caattgagca
     1261 ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct gctttgctgc gatctaaaat
     1321 aatttttcct tctttggtat ctacaatcaa gctaagtcca tttcctttac tatcagagaa
     1381 aagaagtaac tccgatttct gatttggagg aaaagttagt tcaagctcat aagtattatt
     1441 tgtttctgct tttgctgtaa attgttcaga actagcacgt aatgatgtaa tagcttctac
     1501 tggatattgg taaagtttac catcttttat tgaaagttct tttacaagac tcattgctcc
     1561 ctgataatca tacttatcag ttggataatc gatatcaggt agtccaatcc aactgacaat
     1621 cagagcacga ccatcaggag tattgaatcc ttgagtcgca tacgcttcaa atccataatc
     1681 aagattttga atttctgaag cgcccacaag ttttactttt tctatgtcaa gcgattgaca
     1741 gatcttaaaa gtattcggat agatattccc ataatttagc tcagatttat ccaacccttg
     1801 aggacaataa atgagaacag gttttttgtc tacaaatact agatttggac actcaatcat
     1861 gtattctgaa ccagtgccgc caaaatcaag gttgccaact tcctcccatg tttccacgtt
     1921 attgtcatcc gctttgtaaa gtttgataaa gcctgatttt tctaaatttt gtgctccaac
     1981 aatcgcatac aattgtcctt cataattaaa catttgtgga tcacgaaaat gatcagttac
     2041 atcagcaggt ttatgaataa gaacattctc acctttcgta atgttgtagt ctttatccat
     2101 ccatgctccg atttgaagtg tatcacgttc ccaattttca tttcgaacgt ttcctgtata
     2161 gagtaagaat agattcccct ctatttcata tgcagaaccg gaataagcgc cgtggctatc
     2221 tcgttttgtg tctggaagaa gttctgctcc tgttacatga aaatgaacta aatcatctga
     2281 ttcattgtgt acccattctt tcaacccatg agcagctcca aatggccaat tttgataaaa
     2341 aagcgtatat tttccgttaa aaaaagaaaa accattagga tcgtttaaca atcctgtttt
     2401 aggttcgata tgatagctag cacgccaagg tgatcgtttt acattatcct taattttatc
     2461 catctcatct ttagtccaat cagaataagc acgatatcgt atttctcgag gtaagttcat
     2521 attttactcc ttcaatttat ctttttacaa ctagtatggt aaacgttttc cgttgaaaaa
     2581 tcaatcattt catttaaaaa aataaactat atgacaaacg tttgacatat ttttaagata
     2641 tgctaaaata aatttgcatt tcagtgaacc gttttcacgt aaaaataaaa aaggagtgat
     2701 tcgaatgaat tacagtaaag tagcttccga cgtaatacaa gcggtcggaa aagataacct
     2761 tattgcagct gcacactgcg ctacacgtct tcgtctcgta ctaaaagatg acacgaaagt
     2821 gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc acatttaaaa ttgatggaca
     2881 atatcaagtg attatcggtg caggtgatgt taatttcgtt tatgacgaac ttatcaaaaa
     2941 aactggactt tcggaaattt caacagatga tttaaaacaa attgctagta aagataaaaa
     3001 attcaacccc attatggcat taattaaatt attatcagat atttttgttc caattatacc
     3061 tgcccttgtt gcaggtggtt tattaatggc attacaaaac tttttaacag ctgctggact
     3121 ttttggacca aaatcaattg aagaaatgta tcctgcgatt gaaggtattt cctcaatgat
     3181 ccaactgatg tccgctgcac cattcatgtt cttaccaatt ttagtaggta tttcagctgc
     3241 taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt ggtatgatca tgacaacacc
     3301 agatcttggt ggctcaactg aatattggaa tatttttggt tatcatgttt cacaaacaaa
     3361 ctatgcttat caagttattc cagttttagc agctgtttgg atattatcta tattagaaaa
     3421 attctttcat aaaaaattgc catcatctat tgactttaca ttcacgccat tattatcagt
     3481 aatgattact ggatttttga ctttcacagt tattggccct gtaatgttat tgttgtctaa
     3541 tggaattaca gacattattg tttggctata taacactact ggatttattg gaatgggtat
     3601 ttttggagga acatattcac ttatagtcat gactggttta catcaatcat ttccagctat
     3661 tgaaactcaa ttactttcag cgtggacaaa tggtatcgga catggagact ttatctttgt
     3721 agttgcatct atggcaaacg ttgctcaagg tgctgcaaca tttgccattt ggttcttaac
     3781 taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt gtctcagcac ttcttggaat
     3841 tacagagcct gccttgttcg gagttaactt aaaataccgc tttccattct tttgtgcttt
     3901 aattggttca ggaattgcta cagcagtagc aggattactt aaagtagttg ctgtttcact
     3961 tggttcagca ggtttcttgg gatttctttc aattaatgca acatctattc cattttacgt
     4021 attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt acttacttct acggtaaaac
     4081 aaaagctgct ggtatcttta aagcagaaga aattgctgaa caaacaccag ttaattcttc
     4141 aaaagtagca acaacagatc caatatctac tgatgttgaa tcaacacaaa caaaagtttc
     4201 atcagaaaca attgttagtc ccatttctgg taaagtgatt agtctttctt cagtaaatga
     4261 tccagtcttt tcatcagaag ctatgggaaa agggattgcc attaaaccaa atggaaacac
     4321 ggtccattct ccagtagatg gtactattca actcacgttt gaaactggac atgcttatgg
     4381 tattaaatca aataatggtg ctgaagttct aattcatatt ggtattgata ctgtatcaat
     4441 gggaggaaaa ggatttacac aaaaagttac tactaatcaa aaagttagaa aaggcgatat
     4501 tcttggagca tttgaccgtt caatgattga gaaagaaggt cttgatgata caacaatgat
     4561 tattgtgacc aatacttcgg attattcaga agtaaatata gtagctgaaa atacagtcac
     4621 tgagggtact gatttacttg aaatcaaata aatatatttc cctgagtaaa gatggaattt
     4681 tctttccaaa atcaaataat atagattaat caaatatttg
//
LOCUS       DAHUVV010000142         4711 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.145, whole genome shotgun sequence.
ACCESSION   DAHUVV010000142 DAHUVV010000000
VERSION     DAHUVV010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.145"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            107..358
                     /locus_tag="NK767_002693"
                     /pseudo
     CDS             107..358
                     /locus_tag="NK767_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            451..1245
                     /locus_tag="NK767_002694"
                     /note="aph(3')-IIIa"
     CDS             451..1245
                     /locus_tag="NK767_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCH6695630.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1655..1738
                     /locus_tag="NK767_002695"
                     /pseudo
     CDS             1655..1738
                     /locus_tag="NK767_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1787..1870
                     /locus_tag="NK767_002696"
     CDS             1787..1870
                     /locus_tag="NK767_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCH6695631.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1995..2732
                     /gene="erm(B)"
                     /locus_tag="NK767_002697"
     CDS             1995..2732
                     /gene="erm(B)"
                     /locus_tag="NK767_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCH6695632.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2995..3858)
                     /locus_tag="NK767_002698"
     CDS             complement(2995..3858)
                     /locus_tag="NK767_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HCH6695633.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3860..4132)
                     /locus_tag="NK767_002699"
     CDS             complement(3860..4132)
                     /locus_tag="NK767_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCH6695634.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4149..4358)
                     /locus_tag="NK767_002700"
     CDS             complement(4149..4358)
                     /locus_tag="NK767_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HCH6695635.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4456..>4711)
                     /locus_tag="NK767_002701"
     CDS             complement(4456..>4711)
                     /locus_tag="NK767_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HCH6695636.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          796 c          865 g         1605 t
ORIGIN      
        1 tttcttggga gttttgagac tactatatgc gtttttttgt tgatttacca acatgtttag
       61 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
      121 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      181 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      241 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      301 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      361 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      421 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      481 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      541 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      601 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      661 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      721 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      781 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      841 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      901 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      961 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
     1021 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1081 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1141 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1201 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1261 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1321 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1381 gcgcctacga ggaatttgta tcgataagaa atagatttaa aaatttcgct gttattttgt
     1441 acatttaact tgacggtgac atctctctat tgtgagttat tagtggtaca gttttcaacc
     1501 gttttaatta taaaaaagtg gtgcattttt aaattggcac aaacaggtaa cggttattgc
     1561 aggtgtattt cttatctatg ggtttaacat ggattttatc attaaaatca tgagtattgt
     1621 ccgagagtga ttggtcttgc gtatggttaa ccctaaagtt atggaaataa gacttagaag
     1681 caaacttaag agtgtgttga tagtgcatta tcttaaaatt ttgtataata ggaattgaag
     1741 ttaaattaga tgctaaaaat ttgtaattaa gaaggaggga ttcgtcatgt tggtattcca
     1801 aatgcgttat gtagataaaa catctactgt tttgaaacag actaaaaaca gtgattacgc
     1861 agataaataa atacgttaga ttaattccta ccagtgacta atcttatgac tttttaaaca
     1921 gataactaaa attacaaaca aatcgtttaa cttctgtatt tgtttataga tgtaatcact
     1981 tcaggagtga taacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa
     2041 aagtactcaa ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa
     2101 ttggaacagg taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt
     2161 ctattgaatt agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaatactc
     2221 gtgtcacttt aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata
     2281 aaattgttgg gaatattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg
     2341 aaagccgtgc gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg
     2401 atattcaccg aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta
     2461 agctgccagc ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta
     2521 cccgccatac cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa
     2581 aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga
     2641 aacacgccaa agtaaacaat ttaagtaccg ttacttatga gcaagtattg tctattttta
     2701 atagttatct attatttaac gggaggaaat aattctatga gtcgcttttg taaatttgga
     2761 aagttacacg ttactaaagg gaatgtagat aaattattag gtatagggca cctctaataa
     2821 ctaccaatta attcacctca aaatgaaatt agaaaaaaac agcgaaattt ttaaatctat
     2881 ttcttatcga tacaaattcc tcgtaggcgc tcgggacccc aacatgagga aatgttttta
     2941 taaaaaaaga gtagttaccg ttttttggta actactcttt tcctttttta aggtttaaat
     3001 acctggaagt ttaggtgttt tgggtattgg tggtgtaggt ggctgcaagc tttccaattt
     3061 ttgttgaatt gctttaaatt caggtgtctc ttggtgttta tttagaacca ttttttgctc
     3121 tattcgttct aaagtcggtt gaatttcttt ccctgatact ttacgattca attctttttc
     3181 taaggtttct tttggactaa tagaaggtgt ttccaaactt gaatagagtt ttactccttc
     3241 tctgttatat agccttatat cgctaaataa gcccgtttta tgaagggttt ctaaattggt
     3301 cggtaagttt ttgacaacaa tatcatgcgc ttgttttggt gttgccctgg ctgtcattgg
     3361 atcatctgca tacatggttt catatcgttc aattgttcct aaatatgagt tgattttagg
     3421 tactgccatg acatacattt ttgtttcata acctttggct tgaagcattg ttgcggtttg
     3481 aataggaacg tctgttgttc gtcctgtacc ttcgatcacc aaattatacc cttgatcgct
     3541 caaacggctt atgatcgctt ctgtcatgcg attagaataa ggggtaacgt gttttactac
     3601 gtctttttca taaagtttca ctagttcatc aaaattaggg tgctgttgtt taaaggtatc
     3661 attatcaatg acaataacat tcccttgtgt ttcttcaaaa attgctgatc gcaaactggt
     3721 tttccctgac cctggttgcc caccaagtaa aaaagcggtt ggcgattcaa ccgctttttt
     3781 tccttgaatc aattcttcta aattatcatt taagcgattc tcaaattgtt tgtcagtaaa
     3841 attgactata tttgccatat taagccactt tctctttatt caaaacttgt tttgagtaac
     3901 gattcattga ccgccaatac tcatgaacgg cttgtaattc ttctaaagaa taatcaaaaa
     3961 gatttacacg ttttgcaagc tttaattggt ttaataaatt gacttccaat aattgagagt
     4021 catttttatt taattcatgg ttcaaaacat agtttaatac tcgattataa acgcttgccg
     4081 ataattcgtt aatgatctct atttcaaatg ttttttcata cgtaactgcc atttcatttt
     4141 cactcctttt aaagtttgtc aggtaaatat ttccgtatat attcttctaa ggcttgatcc
     4201 ataacttctt ttacgtttcc gccattcttt gctgtttcga tttttatgat atggtgcaag
     4261 tcagcacgaa cacgaaccgt cttatctccc attatatctt tttttgcact gattggtgta
     4321 tcatttcgtt ttgctttttg tgcgcctaaa tttcccacaa tcactcactt ctttctattt
     4381 cttcttactc ttattttatc atcaacaatc acaaatcaca agtgatttgt gattaatcac
     4441 ttgtgatttg tgattctatt ctttttcgtt ttctaattga ataattcgct caagcatttc
     4501 aaaaaatacg ttcttataca tggataaaac ttttttgtca tagcctttat gctctgtaat
     4561 gccgttttta gaccaggtac ttactttatt acttcgcttg ataatatttt ggaaaaccaa
     4621 gttatcttct ttatgttgct tatacagttc ttccaggttt gattttatcg ttgcgctgtc
     4681 cgtatcaact aaataaggaa caaaaccgat c
//
LOCUS       DAHUVV010000143         4591 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.146, whole genome shotgun sequence.
ACCESSION   DAHUVV010000143 DAHUVV010000000
VERSION     DAHUVV010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.146"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            148..1137
                     /locus_tag="NK767_002702"
     CDS             148..1137
                     /locus_tag="NK767_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCH6695637.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELIESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1134..1553
                     /locus_tag="NK767_002703"
     CDS             1134..1553
                     /locus_tag="NK767_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695638.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1532..1870
                     /locus_tag="NK767_002704"
     CDS             1532..1870
                     /locus_tag="NK767_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695639.1"
                     /translation="MVAILEVRCDMDKIDLREETVNRIEVMYERLADYLNLSDEDNYS
                     EMLERMMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQI
                     SVINSKEKGM"
     gene            1874..2236
                     /locus_tag="NK767_002705"
     CDS             1874..2236
                     /locus_tag="NK767_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695640.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2459..3865
                     /locus_tag="NK767_002706"
     CDS             2459..3865
                     /locus_tag="NK767_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HCH6695641.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
     gene            4314..4433
                     /locus_tag="NK767_002707"
                     /pseudo
     CDS             4314..4433
                     /locus_tag="NK767_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
BASE COUNT         1598 a          651 c         1042 g         1300 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt attttatagt tttaattgac tagaaaaaaa
       61 ctaaactaga aaggagagca atctattttt tgttatcggc ttttcagcct tttttgtata
      121 caaaatttgt agattcagta tagcaagatg aaaaagaaaa agcaagattt gattaacgga
      181 acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg catttagtga taatcgaatt
      241 tctcccaaaa tgcgtgaatt gatgacaggc tgtggttcat atttagaatt tattgcaacg
      301 attgataagg agaaaaagaa attggtccaa gctcactttt gtaaaaatcg tttctgtccg
      361 ttatgtgctt ggcgtaaagc tcgtaaggat tcgatgatgt tgtcgattat gatgcaagct
      421 attgcggaag aaaagcagta tcaatttatt tttatgacgt tgacgacacc gaatgtaaaa
      481 gcggaatgtt tgaacgatga aattgatttg tttaatcatg cattgaaaaa gatgtttatg
      541 cgcaaacggg ttaagacttc gattaaaggc tatgtgcgaa aattggaaat cacgtacaac
      601 aaaaaacgga atgactataa cccgcatttt cacttgattt tggcagtaaa taaatcttat
      661 ttcacggata aagattatta tatcaagcaa tcggaatggt tagatttgtg gcgggaagtg
      721 accggaaaaa cgggaattaa tccggatggt accgacgaaa ttacccagtt ggatattcgc
      781 aaggtcaaag ggttccaaca agaaaaagca gtacaggaag ttgcgaaata ttcggcaaaa
      841 gattttgaac tgatcgaaag tcaggaagtg tttgacacat tctatcatgc aatgaaaggt
      901 cgtcagttga ttacgtttaa cggtgtattc aaagaataca agaaaaaata tgagtcgggt
      961 gaccttgatc gttataagaa aaaagatgag aatgaatatt tttggttttt gactgcttcg
     1021 tggatgagga aagaatcgaa atatgcggtt gattatcgtg agatgagtgc tgatgaaaga
     1081 aattattttt caggaatgta tgtcgatgaa aaatctgttg agggggaaaa tgaatgactg
     1141 attcaacgga taaaatgaca attaaggcgc ttgctgatga actaggaata accaaaaata
     1201 cagtgagaaa ttatctagtc aaagccggat atgatcttgc tcggtacaag aaaaatggaa
     1261 ttatcttgct agacaaagag ttgattggtg ttgctcggtc aatttatcgg tcaaaaggtg
     1321 gtcaattgac tggtcattta ccggtcaata gcacgggcag tgattttcct ttggaaaaag
     1381 agaatgagtt tttgcgtgat caattagctg agaaggacaa acatatttct gaattaacag
     1441 aaatgattcg tgatcaacaa aaattgaccc tgcttcagca acaaaaaaac gatcagttgt
     1501 tactagaaaa tcaagaatta aaaaatcgaa aatggtggca attctggaag tgagatgtga
     1561 catggataaa attgatttga gagaagaaac agtcaataga attgaagtga tgtatgaacg
     1621 gttagctgat tatttgaatc tttcggatga agataattat agtgagatgc ttgaacgaat
     1681 gatggatgtt ctcaactcgg ttatcaatgc tgatctagca aaatcttcag ttttgactaa
     1741 tgaatttgtt cataagcatt tagcgcaaac aactgatgaa gctgaaaaag aattttggtt
     1801 gaaattaatg agtcatatta tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa
     1861 ggggatgtag tttatgggaa tgactttagc agatattaaa gtgcaagaag aaaaattaaa
     1921 aaaagaacag gcgaaacttc gccaacagaa gaaacgattg attgagaaag aaaatgctgc
     1981 aatgggcaag gctcttcgca agcgttttgg gtttgaatct tttgctgatt ttgagcggtg
     2041 gctatcgtcg atttctgacg atcagcaaca cgaaaaatca gtgggtaatg agttgcgagc
     2101 tgatgaaaaa tcagcaccaa gcgaacggaa ttaccctaac gaatctgacg gaatattcgg
     2161 agggataccg aatagaaaaa gtcagatgag tgagagtgga tttgcgtcaa tgtttagtga
     2221 cgtaaattca cgataagcga atattttgca tgaagcaaaa taaagcgtaa aaggagttga
     2281 gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacggcga aaaccctcgg
     2341 agagcgtttg gaattgtgac agtcatgtca caattaggaa aacgtttact ctttgacaaa
     2401 gagtaacaga aaggtttaca ctctttttat tagcgacagc gcgaaggagg ggtttaactt
     2461 ggggtttagt atttcgttca aaaagggaac aaagaataca agcatttctc ataataatcg
     2521 tgagttaact gatagccaaa aaatcaatga ctggcataag catattgatt tcacaaaatc
     2581 ggatgaaaat atctatttgg aacaaataaa tattcgtgaa aaatatgaag aattattcgg
     2641 tgaagccgta gaaaaatata atgcaaagca gaaaagagcc gatagaaaaa ttgaggacta
     2701 tttagcaaag gttcgcaagg ataaaaaaat ggaaccacag cgagaattta tcgtgcaaat
     2761 tggtactctt gatgattttc gtactactcg tgatgatggt tcttctactg gcatatcgga
     2821 acaacaagca gaacaaaatc gagtgattgc caacaaaatt ttagttgagt atttcaaaga
     2881 atttaaagaa cggaatccta gtttatccgt ctacaatgct gttattcata atgacgaagt
     2941 aagtccgcac ctgcatttga atattgttcc tgttgctgaa gggtataaac ggggtgttca
     3001 aaagcaacca agttttaaca aagccttgca acagcagggt ttacgagcag ataaagagaa
     3061 tggtcgagtg atttggaata attttaggaa tcaggaagtt gagtcaattg aacgtttgat
     3121 ggcagatttt ggttgggagc gtgagttagt cggtactaac aaaatcaaag atattcatga
     3181 atacaaagaa atcatgagcg aaatttccga attgcgtcaa acggccgtaa aagagcgtga
     3241 gagcgtttct gatgagttga ggtatatttg taggcaaaga gaagaaaacg agcgtgagaa
     3301 ggctctggtg gcttctgaga gagcgaagat tgaagaagag aagaaaaata tgctgaatgt
     3361 aaaaaaagag atggcggtca ttttccaaaa agatatggtt cctttagaaa gtttcaagag
     3421 gcttcctgat ggttcgtatc atttatctgg gaatgatttt gttgatttgt atcaacgagc
     3481 tacgagagca acaaatgcca agagaaatgc tgataacatg agtgagcaat atttacagat
     3541 gcttgagcaa aatacagaat taaatgagta tgtagctgca ttggaagaag agcgtcattt
     3601 gtctggtaga aaacaattag cagatttaca agtggaaaat aaaaagctta aagaagaaaa
     3661 tcgctcttta cgccagcgtt tagagcgatt aaaaaacgca tttcaaaaat cgatcactcg
     3721 tttgtctatc cgttttgggc ttgcagaaaa ggatatgggg ctttctgagg aattagaagt
     3781 attagagcag gagaaaacct tattttatcg tcaaaacgtc tcacagcata aattagagcg
     3841 ttctgaagat gagatcgagt ggtagttacg atgtgcagtc aacggtaccg gaggcggttt
     3901 gtgccgagga taaggagttg acggctaaac cactagccta ttaagctgac atttgctaaa
     3961 aaatggccag tccatttttt ggccaactta gcgttagcga agtttggggg cgcccttttc
     4021 aaagggtgcg gttttatgtt tttggatatt tgcatagcta aagagaagtg ctattattga
     4081 gttatcaaat aaataaactg aaaaaagagc aaaaaaataa agtcgagcaa cgctggacac
     4141 gttctcgacc tttacggaaa ctaatcacac tagctccgta actcaaattc attatacgaa
     4201 taatttcagg tttcttcaag actagagatt gctggttatg aattttttag agttacgtga
     4261 actctggaaa attctttcca ccaatctctt ttttctttga ggaggaacta aatttgagta
     4321 aaaaaggcac gatcataaat gagtgggaaa aattaacagc cccctctaac aggcggttaa
     4381 atgtaaaaac tttttacacc cccttaaaaa tgcagtggtc gattgatcga attttatgtc
     4441 tttgggtttt ggggttttaa aacgaaaaaa agggggcgat tttcgccctc ttttttgatg
     4501 ttttttctta tcttgatact ataggcaact atttctgcga agaaatcggg gctatagtat
     4561 gggtctatcc cttgtggctc taaggaagaa c
//
LOCUS       DAHUVV010000144         4533 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.147, whole genome shotgun sequence.
ACCESSION   DAHUVV010000144 DAHUVV010000000
VERSION     DAHUVV010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.147"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            512..1069
                     /locus_tag="NK767_002708"
                     /pseudo
     CDS             512..1069
                     /locus_tag="NK767_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1127..3166
                     /locus_tag="NK767_002709"
     CDS             1127..3166
                     /locus_tag="NK767_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HCH6695642.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3275..4426)
                     /locus_tag="NK767_002710"
     CDS             complement(3275..4426)
                     /locus_tag="NK767_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCH6695643.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1652 a          829 c          873 g         1179 t
ORIGIN      
        1 tataaaaaaa cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt
       61 tgcaacggga ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta
      121 aatttcacta tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac
      181 tcgtaaatag aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt
      241 tgcccagaaa cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt
      301 tccaatagct aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg
      361 atgctaggtc agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac
      421 tgacttattt gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag
      481 gagatgcggt gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca
      541 atacaaccag aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga
      601 tattccaggt tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt
      661 attaaatcaa acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac
      721 atttcaagat tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc
      781 agaaaaatct ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg
      841 taatacttta ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat
      901 cagaaggcag cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat
      961 attcaagcta cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga
     1021 aagtgtgctt ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt
     1081 ataaatagtg aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga
     1141 caagcacggc aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc
     1201 tgcgggtggc ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg
     1261 agaaaagaca gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc
     1321 agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga
     1381 tcaataccag aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa
     1441 agtagataaa aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc
     1501 attaggtgaa ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac
     1561 cacgatcaac tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag
     1621 cctgaccaca caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc
     1681 aacaacaggc aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga
     1741 aaaaacagcc aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa
     1801 tcaggcgatt tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga
     1861 tggagcaacg ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta
     1921 tcctttgggt gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga
     1981 tattgataag aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat
     2041 ggcttttgat aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga
     2101 cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt
     2161 gacaatcgat gcgaaggcac aaaaaacagc ttttgacagt ctaggaggaa aagctggatc
     2221 aactgttgcg acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta
     2281 tgatccaaac aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa
     2341 tcctgaacat ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa
     2401 aataattaca gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac
     2461 gatcaacggg cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt
     2521 cagtagtgat gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata
     2581 tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt
     2641 catttttggt gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga
     2701 agatagcttt aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct
     2761 aattaatcct atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt
     2821 ctatcctaaa ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac
     2881 agcagtacaa acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc
     2941 acattctctt tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa
     3001 agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa
     3061 ccaaggttat atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa
     3121 acgagcatcg gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta
     3181 agtacagtaa aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc
     3241 acgatgtctc tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa
     3301 aatatttgag aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg
     3361 aatagtactg tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca
     3421 tttctccacg cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg
     3481 caatctgagc accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata
     3541 tagaaataaa gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga
     3601 caaaaaatac tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt
     3661 ctttaacctt tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa
     3721 gcccaacaag atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa
     3781 tagaagaatt tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga
     3841 ctaataagaa aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag
     3901 cattgctaga agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta
     3961 aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt
     4021 ttagttcttt taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa
     4081 agaaaatcgc ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa
     4141 ccgggaagat gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa
     4201 gagataaatc actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa
     4261 agtcatggac tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac
     4321 caacaagtaa ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa
     4381 tcgttgttga aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt
     4441 tgtataaaat atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata
     4501 ctcacaaaaa aaccaacaat ccaaaagatt ttt
//
LOCUS       DAHUVV010000145         4435 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.148, whole genome shotgun sequence.
ACCESSION   DAHUVV010000145 DAHUVV010000000
VERSION     DAHUVV010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.148"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            59..913
                     /locus_tag="NK767_002711"
     CDS             59..913
                     /locus_tag="NK767_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCH6695644.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="NK767_002712"
     CDS             1010..3259
                     /locus_tag="NK767_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCH6695645.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4435
                     /locus_tag="NK767_002713"
     CDS             3262..>4435
                     /locus_tag="NK767_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6695646.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
BASE COUNT         1559 a          629 c          812 g         1435 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actagagtaa cccgtggtgc tagtt
//
LOCUS       DAHUVV010000146         4396 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.149, whole genome shotgun sequence.
ACCESSION   DAHUVV010000146 DAHUVV010000000
VERSION     DAHUVV010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.149"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..93
                     /locus_tag="NK767_002714"
     CDS             <1..93
                     /locus_tag="NK767_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCH6695647.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(151..1371)
                     /locus_tag="NK767_002715"
     CDS             complement(151..1371)
                     /locus_tag="NK767_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HCH6695648.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1391..2002)
                     /locus_tag="NK767_002716"
     CDS             complement(1391..2002)
                     /locus_tag="NK767_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCH6695649.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(2045..2977)
                     /locus_tag="NK767_002717"
     CDS             complement(2045..2977)
                     /locus_tag="NK767_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCH6695650.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3398..4258)
                     /locus_tag="NK767_002718"
     CDS             complement(3398..4258)
                     /locus_tag="NK767_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6695651.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1257 a          882 c          727 g         1530 t
ORIGIN      
        1 cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact
       61 gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag ggactttatt
      121 ctttatttaa aactttgcaa cagaaccttt ttattttaca gaagcactcg aaactgcatc
      181 tactgcagtt tcatcgtgtg tgctggcgct ggttactgca tctgtttttc caccatgaac
      241 agatgcactt gaaaccgcat cttcatttcc aacaagttgt tgacccgttt gttgtaaata
      301 ccactcaatg attggtttga aatcaagaat gtattcattg attccagcat actctccatt
      361 ttcatcatgc attgcttggt aattatgcac aatatatttt tctggcactt gttttggtac
      421 atgaacacgt accgcatctg ttgctccgct tcgcagttgt tgaattaccc actctacatt
      481 tttatacgat ttttctgggt gacagttggc taatggattc cctacctgac cgggcacacg
      541 agcagctaac atctctttcg cttcttggcg caaattataa tataaaaatt ggttattatt
      601 atctgcaaaa gttaattcca tcggcatcga atctaaaaag taattgattt gatccactgt
      661 caataaccca cgatttaatt tgacgtaagt atttccagaa acggcaccga ctgcttcaga
      721 agctttttcc aaccaatgat catctgacat atcaacatcg ggtattgtcg tagcaattgt
      781 tcctgtcgca tgcaaatctt ctggtggaag ctttttaggc cctgctaagg tagacacgac
      841 tttaatgaat tttacaaaac ttgctaatgt actgcctaaa aagtcaacag ttcgtggatc
      901 aatcaagcga tcttcttggt caaatgcttc ttttgctttt cccaataaaa attcatttcc
      961 gggcatcact aatgcattca cgcctggtgc gtccaaaatt tggcgcaagt gtaattgcgc
     1021 gcgtgaagtt ccttgatcgt agtaagaagc acccacaacc attacaggtt tattttcaag
     1081 tggatggatt ttaaatgata gccattcaag tgcacttttc aatcctgctg gaaccgtatg
     1141 attgtgttct ggcgtagcaa taattacccc atcagccatt aagattttgc gatttagctg
     1201 ttgaatagca ggactatttg tttgatcatc atcttgattg aacagaggta aatctttgat
     1261 ttcaatgatt tctaaattaa atagttcatg atattgtttt tggataaatg ttaataattt
     1321 acggttatat gaaagttcag ctgctgatcc gacgatacct attaatttca tctttgtttg
     1381 ctcccctcga ttaaatcgtt tcccatgaaa agctttcggc ttcacgttta tttgcttggt
     1441 gcgcatgttg caattgacta gtgattgtga caaagagtaa gaaatctttg aatagttcac
     1501 gtaattttgc gacttgttct tcatcaatga gtcgtccttg ctcatcaaag gcttggagtg
     1561 aatgacccaa taaaaattct gagcttggca taattcgtgc ctttaactct ggtgaatcca
     1621 atatttggcg caaatgtgct tgtgcgcgtg aagaacctaa cgtgccataa gacgctccgg
     1681 taatcatcac tggtttatcc acaaacggaa aaataccata tgaaagccaa tttaatgcgt
     1741 taatcaatga agcaggaacc gcatggtcat actctggtgt gctgatgatt acgccatctg
     1801 cttgttcaat ttttccagca atttctttaa caacatttgg tagttctgtg ttttcaggtt
     1861 tgttaaatag tggcagctcc ttgatttcga caatttcgat ttctgcctca tttacaaaat
     1921 aatttttcat aaactgtaat aattcacggt ttgttgaccg cgttgaattt gttcctacta
     1981 atccaataaa tttcatcttc attctctttc ctttctaaca tcgatttctg actgttcgca
     2041 cgtactaaat tttttcaaat ccttttgtat acaagaattg attctctttt gttacaactg
     2101 ctccctcgat acccggctgt tcttcaagcg ctttcaacac ttctaaagaa gatgctccaa
     2161 ataaacgtgt agtccagatt tctccatcta cagaagaatc agacaaaatc gtcaggcttg
     2221 ctacgtcatt tttaactgga aagccagttt tcggatcaat gatgtgatga tacactgacg
     2281 aaccattttg tagataacgt tcgtagattc cagaggtaac taaagattgg ttgtttagtt
     2341 taaccaccgc aagcgactct tcacgtgaac ctttaggatt tcgcaaacca atacgccaag
     2401 caccatccga acgtcttggt gattcaccaa aaacaacgat atttcctcct aagttaacta
     2461 aagcaaacgc tgcatttcgc tctcggcaat gtgtaattaa tttatctgca atgtaacctt
     2521 ttgctaacgc ccccaagtca atcatcattc ctggtttggt taaaaaaacg gttttcattt
     2581 tttcatctaa ttgaatttgg tgtggatccg ttagcagaag taactcagtt atctctgctt
     2641 ttgttggaac caatgcatct tcaaatccaa tgtgccattt ttgtacaacc ggaccaatag
     2701 caatattcag ataactattt cttgcacaac tatgtgcttt tcctaatttg atgagctcaa
     2761 aaagttcagg atgtacaatc acagcttgtc gtccagcagc ttgattgatg gccattaatt
     2821 cagaagttgc atcatgtgcg ctgaagcgat gttcataaac gtgcaaccat tcgcctaact
     2881 ctgctagtaa tcgactaggc tcttcatgcc caacttgtaa tgtaattgtt gtccccatca
     2941 tttgccagat tttcgtttct tcgatcacct gattcatttt gtaccttctt tacttgaact
     3001 acggattaca tgagtaatta gtttttattt ttgttactgt tttcacaacc agatgatacg
     3061 cctgctagat ttatttgtca accgatttta tatatttttt tatataataa ggtaatttta
     3121 atataaaatt ttatattgta aaaaaacaaa taagggaaac aaacaacaca tttttgtgtg
     3181 ttgcttattt ccctctaact atcgttttct atttgatggt taacctactt aaaataaata
     3241 ctgcaacgac aaacggagat caagcatgct ttgtaacgta ttttacgata attacgactt
     3301 tattttatta ctctatcttg ctgccatacc cgtctatccc taatcatctt tggtgaattt
     3361 tttcaaagtt agcataacaa aaataagtat tttttcctta gttaatcaat agtatcattt
     3421 ttttcatatg atttaattca tcaattaatt ttttgttttt attaatttta gcaaaagcta
     3481 gtgcatcttg aatcaattcg agcacttctg ccttcgactt atttttagcc tgtgcgttct
     3541 tcgctaattg ggcagcagct cgtgctaagt aataagtcaa atggttatcc gaacaaatat
     3601 cgattgcgta atttagtagt gcatcacttg tttccaaatc accgtcttgc gcaaaaaaaa
     3661 caccacaatg aaagacgata tttaacaccc gccagatatc ttctgttgta cgaatcggat
     3721 acgtataaat tttttcaaac actcgattga aataaacttc tgctttttcc aactcttttt
     3781 ctctagcata gaccattcca atacctgtat atgccaataa acgaaacaac gtgttggctg
     3841 tttctgccat caacaactga tcaaaatgga acaacacatc cgtaattggt gcattcgtaa
     3901 aaatcatttc aaatcctttt agatagtagt aacgtgcaat cagctcagac tcgtcaatta
     3961 ccttgggatc aatggctgct aaaatctgtg tcaattgttc atattcactc gtaatcagtg
     4021 aaaactctgc tttttctagc tgttcatcaa gttgtgtatt gatcacgttc atcgaagaaa
     4081 aaagttcaac taacggaaga ttgattcgat tacataattt gattaaaatt tttaacgaag
     4141 ggacttgacc attgttctca aatttactta atgttgcttg tgtacaaatt ccttcagaca
     4201 cttctttttg agataagttg gcagcctttc ttgccgtaat aaatttttca atgttcatta
     4261 ttttctctcc aatcagacct cacacgaaaa tcatttctgt ctcgatttta gcatgttttc
     4321 tttgggaatg ctttaggttc tgttgcaaag ttttaaataa agaataaagt cccttacggt
     4381 atctatgatt taagct
//
LOCUS       DAHUVV010000147         4217 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.150, whole genome shotgun sequence.
ACCESSION   DAHUVV010000147 DAHUVV010000000
VERSION     DAHUVV010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.150"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            75..503
                     /locus_tag="NK767_002719"
     CDS             75..503
                     /locus_tag="NK767_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCH6695652.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="NK767_002720"
     CDS             490..732
                     /locus_tag="NK767_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6695653.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="NK767_002721"
     CDS             complement(1032..1247)
                     /locus_tag="NK767_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695654.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="NK767_002722"
     CDS             1354..1668
                     /locus_tag="NK767_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6695655.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="NK767_002723"
     CDS             1772..2950
                     /locus_tag="NK767_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6695656.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            3353..3646
                     /locus_tag="NK767_002724"
     CDS             3353..3646
                     /locus_tag="NK767_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HCH6695657.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1446 a          627 c          820 g         1324 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
     4021 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
     4081 cattttttaa gtgaatacaa gatcagctag ttgaacaccg tttattcgtg atgagggaca
     4141 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
     4201 gaaaaatagt agataat
//
LOCUS       DAHUVV010000148         4143 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.151, whole genome shotgun sequence.
ACCESSION   DAHUVV010000148 DAHUVV010000000
VERSION     DAHUVV010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.151"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..397
                     /locus_tag="NK767_002725"
     CDS             <1..397
                     /locus_tag="NK767_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase WbuB"
                     /protein_id="HCH6695658.1"
                     /translation="INTNNIKNTILIDSLSKNEYKKVESIADIGLVFLDKKFTIPNIP
                     SRILGYMESKKPILAAVDKHTDLNEMIKTGKFGYCSEHGDLNAFLSNMERLKDEKIRN
                     ELGKNAFIYLKNNFDVKRSYSIIMNHFEL"
     gene            413..1435
                     /locus_tag="NK767_002726"
     CDS             413..1435
                     /locus_tag="NK767_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCH6695659.1"
                     /translation="MFKDKILLITGGTGSFGNAVMKRFLDSDIKEIRIFSRDEKKQDD
                     MRKKYNNDKLKFYIGDVRDINSVKNAMYGVDYVFHAAALKQVPSCEFFPMEAVKTNIM
                     GTDNVVTAAIEMGIQKVICLSTDKAAYPINAMGISKAMMEKVFVAKARTVTDERTMIC
                     GTRYGNVMASRGSVIPLFIDQIKNGKPLTVTDPGMTRFLMSLEEAVELVVFAFQNAKA
                     GDIMVQKSPASTVGDLAQAIKELFHADNEIHIIGTRHGEKRYETLLTKEEYLVAQDMG
                     DFYRVPADTRDLNYDKYFVEGSKELTQEGEYNSDNTQRLSVEQIKERLLELDYVRKEL
                     KEWQNQ"
     gene            1432..2541
                     /locus_tag="NK767_002727"
     CDS             1432..2541
                     /locus_tag="NK767_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HCH6695660.1"
                     /translation="MNILVTGSNGFIGKNLVAELKNEGFTDILSFDRDTPVHLLDEYC
                     EKADFVFHLAGVNRPKDDAEFMTGNFGFTSELLEKLKEHGNTCPVMISSSIQAELNNS
                     YGKSKKAGEDLLKKYQKETGAPVYIYRFSNLYGKWSQPNYNTVVATFCYKIARDEEVT
                     VNDPSAIIELCYIDDVVKELLHCLNGQPYTENGYCAVRETDEVTVGELKELITSFKNS
                     RTNLSVPNLARRVEKNLYSTYLSFLPEDEFSYFLKMNVDNRGSFTEFLRTKDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGEGVIRFRKVDSNEIIEYFVSGEKLEVVDIPVG
                     YTHNIENLGSTDMVTVMWVNEPFDPEHPDTYYVEV"
     gene            2545..3669
                     /gene="wecB"
                     /locus_tag="NK767_002728"
     CDS             2545..3669
                     /gene="wecB"
                     /locus_tag="NK767_002728"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCH6695661.1"
                     /translation="MKKLKVMTVVGTRPEIIRLSAVINRLDRSEAIDHILVHTGQNYD
                     YELNEVFFEDFKLKKPDYFLNAATGTAIETVGNILIKIDPILEEVKPDAFLVLGDTNS
                     CLCAIAAKRRHIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINLTYSDIAREYLLRE
                     GLPADRVIKTGSPMFEVLNSRKDDIQHSDVLNRLGLKEGQYFVVSAHREENISSETNF
                     LHLVDSLNTIAETYQLPIIISTHPRTMKMIEAKEIKFHELIQTMKPMGFNDYNKLQIN
                     AKAVLSDSGTISEESSILNFKALNIRQAHERPEAMEEASVMMVGLEKERIMQGLEVLE
                     TQQKDTLRHVADYSMPNVSEKVLRIILSYTDYVNRVVWGR"
     gene            3666..>4143
                     /locus_tag="NK767_002729"
     CDS             3666..>4143
                     /locus_tag="NK767_002729"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCH6695662.1"
                     /translation="MRIVVNDIAASEGGAMSVLRDLHSDIISSGDENEWIFLLGEKFI
                     EESDNIKVLTFPKIKTSWIKRLDFDFISGKKIINKLQPDIYLSLQNTATIGVKAPQYV
                     FLHQPLPYQKEKNFSFIKKKEFKMATYQHVIGKIYSILFKKTKATIIVQTQWMKENV"
BASE COUNT         1536 a          627 c          806 g         1174 t
ORIGIN      
        1 tataaatact aataatataa aaaatacaat tctaattgat agtttatcta aaaacgaata
       61 taaaaaagtt gaatccattg cggacatagg attggtattt ctagataaaa aatttactat
      121 ccccaatata ccttctagaa tattaggata tatggagtca aaaaaaccta ttttagctgc
      181 tgtagacaag catactgatt taaatgaaat gataaagact ggaaaatttg ggtattgttc
      241 tgaacatgga gatttaaacg catttctaag taatatggag cgattaaagg atgaaaaaat
      301 aagaaacgaa ttaggtaaaa atgcttttat ttatttgaaa aataattttg atgtaaaaag
      361 aagctattct ataattatga atcattttga attataaaaa ggagcaattt aaatgttcaa
      421 agataaaatt ttattaatca caggcggcac gggttctttt gggaacgcag tgatgaaacg
      481 ctttttagat tcagatatca aagaaatacg tatcttttct cgtgacgaga aaaaacaaga
      541 cgatatgcgg aaaaaataca ataatgacaa attgaagttt tacattggag atgttcggga
      601 catcaacagt gtcaaaaatg cgatgtacgg cgttgattat gtcttccatg cagcagcact
      661 taaacaagta ccttcttgtg agtttttccc tatggaagct gtgaaaacaa atatcatggg
      721 aacagataat gtcgtaactg cagcaatcga aatggggatt caaaaagtta tttgcttgtc
      781 cactgataaa gctgcttatc caatcaatgc tatggggatt tctaaggcta tgatggaaaa
      841 agtatttgtt gctaaagcaa gaaccgttac tgacgaacgc acgatgattt gtggcacacg
      901 ttatggaaat gtaatggctt ctagaggatc agttatacca ttattcattg accaaataaa
      961 aaatggtaaa ccattaacag tgacagatcc tggcatgaca agatttttga tgagtttgga
     1021 agaagctgta gagttagttg tttttgcctt ccaaaacgca aaagcagggg atatcatggt
     1081 tcaaaaatca ccagcttcta ctgttggcga cctagcgcaa gccatcaaag aattattcca
     1141 tgcagacaat gagattcata tcatcggaac gcgacatggc gaaaagcgtt atgaaacact
     1201 actgacaaaa gaagaatact tagttgctca agatatgggt gatttctatc gtgttcctgc
     1261 agatacacgt gatttgaatt atgacaagta tttcgttgaa ggaagcaaag agttgacaca
     1321 agaaggagaa tacaattcag ataatacaca acgtttatct gtagaacaaa tcaaagaaag
     1381 acttcttgaa ctggactatg taagaaaaga attgaaagag tggcaaaatc aatgaatatt
     1441 ttagtaactg ggtcaaatgg ttttattgga aaaaatttag ttgctgaatt aaaaaatgaa
     1501 ggattcacag acattcttag ttttgataga gacacgcctg tacatttatt ggatgaatat
     1561 tgcgaaaaag cagactttgt ttttcattta gctggagtga acagaccaaa agacgatgct
     1621 gaattcatga caggaaattt tggttttact tcagaattgc tagaaaaact aaaagagcat
     1681 ggcaatacat gtcctgttat gatttcctct tctatccaag cagaacttaa taattcttat
     1741 ggcaagagca aaaaagccgg agaagatcta ttaaagaaat accaaaaaga aacaggagca
     1801 cctgtatata tttatcgctt ctcaaactta tacggtaagt ggtcgcaacc aaattataat
     1861 acagttgtag ccacattttg ttacaaaatc gcaagggacg aagaagttac ggtaaatgat
     1921 ccgtctgcta tcatagagct ttgttatatc gatgatgtcg tgaaggaact actccattgt
     1981 ctgaacggac aaccttatac agaaaatggg tattgtgctg ttcgtgaaac ggatgaagta
     2041 acggtcggcg aattaaaaga actaattaca tctttcaaaa atagtcgaac gaatttgagt
     2101 gttcctaatc ttgcgagaag agtagaaaaa aatctgtaca gtacgtactt aagcttctta
     2161 ccagaagacg aattttctta ttttctaaaa atgaacgtag acaatcgagg atcgtttact
     2221 gaatttttaa gaacaaaaga ccgcggtcaa gtatctgtca atatttccaa accgggaatc
     2281 actaaaggaa accattggca tcatacaaaa aatgaaaaat tcttagtagt aagcggagaa
     2341 ggtgtaatcc gttttagaaa agtagactca aatgaaataa tcgagtattt tgtttcagga
     2401 gaaaaattag aagtagttga tattccagta ggttatacgc acaatatcga gaatctggga
     2461 tcaacggata tggtgacagt gatgtgggta aatgagccat ttgatccaga acatcctgat
     2521 acttattatg tggaggtcta atcaatgaaa aaattaaaag tgatgacagt agtaggaacc
     2581 cgaccagaaa tcattcgttt gtctgctgtt atcaaccgtt tggatcgatc agaagcaatc
     2641 gatcatattt tagtacacac tggccaaaac tatgactatg agctaaatga agtattcttc
     2701 gaagacttca agctaaagaa acccgattat tttctaaatg cagcaactgg tacagcgatt
     2761 gaaacagttg gaaatatttt gatcaagatc gatccaattt tggaagaagt aaaaccagat
     2821 gcattcttag tattagggga tacaaatagc tgtttatgtg caattgcagc caaacgtcgc
     2881 catattccta tttttcatat ggaggcagga aatcgctgtt tcgaccaacg agtaccggaa
     2941 gaaacaaatc gtaaaattgt tgatcataca gctgatatta atctaaccta tagcgatatc
     3001 gcgagagaat atctcttacg tgaaggatta ccagctgatc gtgtaatcaa aacaggaagt
     3061 ccgatgtttg aagttctcaa ttcaagaaaa gatgacatcc aacattccga tgtccttaat
     3121 cgcttagggt tgaaagaagg acaatatttt gttgtttctg ctcaccgcga agaaaatatc
     3181 agctcagaaa caaatttctt acacttggta gatagcttga atacaatcgc tgaaacttac
     3241 caattgccaa tcatcatcag tacgcatccg cgaacaatga agatgatcga agcaaaagaa
     3301 atcaaattcc atgagctgat ccaaacaatg aaaccaatgg ggttcaatga ttacaataaa
     3361 ttgcaaatca atgcaaaagc tgtactaagc gacagcggaa cgattagcga agaatcttcc
     3421 attttgaatt tcaaagcatt aaacatccgt caagcacatg aacgaccaga agcaatggaa
     3481 gaagcaagtg tcatgatggt aggattagaa aaagaaagaa tcatgcaagg actggaagta
     3541 ttggaaacgc agcaaaaaga tactttgcga cacgtcgcag attattcaat gccaaatgtt
     3601 tcggaaaaag tattgcgtat catcctttcg tatacagact atgtgaatag agtggtgtgg
     3661 ggaagatgag aattgttgta aatgatatcg ctgcttcaga aggtggggca atgtcagtat
     3721 tgagagactt acattctgat ataataagta gtggtgatga aaatgaatgg atttttttac
     3781 taggtgaaaa attcatagaa gaaagtgaca atataaaggt actaactttt cctaaaataa
     3841 aaactagctg gattaaaaga ttggattttg attttattag tggaaaaaaa attatcaata
     3901 aattacagcc tgatatctat ttatcattac aaaatacagc tactattggt gtaaaagctc
     3961 ctcaatatgt ttttttacat caaccactac catatcaaaa ggaaaaaaat ttttcgttta
     4021 taaaaaaaaa agagtttaaa atggctacct atcaacatgt tatcggaaag atatattcta
     4081 tattgtttaa aaaaactaaa gctactatta tagtgcaaac tcaatggatg aaggaaaatg
     4141 ttt
//
LOCUS       DAHUVV010000149         4107 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.152, whole genome shotgun sequence.
ACCESSION   DAHUVV010000149 DAHUVV010000000
VERSION     DAHUVV010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.152"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..888
                     /locus_tag="NK767_002730"
     CDS             <1..888
                     /locus_tag="NK767_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HCH6695663.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            complement(930..1622)
                     /locus_tag="NK767_002731"
     CDS             complement(930..1622)
                     /locus_tag="NK767_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCH6695664.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            1617..1883
                     /locus_tag="NK767_002732"
     CDS             1617..1883
                     /locus_tag="NK767_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCH6695665.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(2059..2628)
                     /locus_tag="NK767_002733"
     CDS             complement(2059..2628)
                     /locus_tag="NK767_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCH6695666.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2702..3025)
                     /locus_tag="NK767_002734"
     CDS             complement(2702..3025)
                     /locus_tag="NK767_002734"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695667.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVITDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(3179..3991)
                     /locus_tag="NK767_002735"
     CDS             complement(3179..3991)
                     /locus_tag="NK767_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695668.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
BASE COUNT         1277 a          765 c          702 g         1363 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc acaaagaggg ggggattttt tatcgttgtg gaatatttaa ataccaacgc
      961 ttgtcatgaa aatcttgtgc tccaccctta gtatttcctt ctggatcgtt tgtcgcacgc
     1021 atcatgacgt atactttctt attagggaaa tcacgcatat tgaaagatac atgatagcca
     1081 acatttccta aagtattata agcttgattt acgtctggtc tataaatccc atcagctctt
     1141 actctagcta gttcttttcc agtattgtag tccataatga aaatatactc gtatttataa
     1201 ttagcaatgt gccatccagc tacatgcaag tttgcgtttt cgatttctcc gaactgatca
     1261 atgtgggcgt aatttgttcc atctgtcagc gtagaatttg cagcacctgc tcgagttgga
     1321 tcaattactg gtttatcctc cgaagtagtt ggattatcat ccgtaaaacc atgagccaaa
     1381 tcataggcta gtttttcttt acttacaccc atttcagaaa gataaccgta aggatctgta
     1441 tgatcgcccc aaatattttg tgttacccat aaatgcgatt tgattcctgg ttggttataa
     1501 ggagtgtcta atgttaatgg aataccatat ttcattgctg aatctcttgc caattcaacg
     1561 tatactttat agttcttttc aaaagtttct ttatcatgtg tgtgttgtaa ctcgatttgc
     1621 acaggacttt tggctcacga ggttgtccta ctctacatct tgctgataga tatgctgaaa
     1681 ggaaaacacc aacattatgt tttgcttttt cagctagaac gagacaagca ggaatacctt
     1741 tagaagatat caaagatttt ttaggtcata aagatgtttc cactacacaa gtctatgccc
     1801 atatttctcc cgaagtgaaa aaaagatcaa tgaatcaact agaaaactat atagaagagc
     1861 aaataaaaaa gcactcaaat tgagtgcttt tttaagctca ctatcactaa aactatcact
     1921 aattttattt ataagcgaac atgtttctat cactgatttt ttaaatctag cgaaaaaccc
     1981 ttatcttaaa ggcgttcgtt caattctttg gcaaggtctt caaagcctgg cttgcctaga
     2041 agtgcgaaca tggttttctt atagctcatg tagaaaaatt ttcttgatga tgattttatc
     2101 catcaattga gattgactgt tttgctctcc tatgatttca gctatgattg tccgattttg
     2161 cggttttctt cttaactggt agttgatttt cccagtttcc atcaactcgc taagcatatc
     2221 cacgactccg ccaaatccgg tctttttcgt aataaaacct agtgctacca ttcgttcatc
     2281 tagatttttt gctgtaatcg tcacaccagt aatgagcgag tgctgtaatg tttgggccag
     2341 ctcttccatc ccttcagcat tggatacgac ggaagctgtt ttgttaatag gttcttcgtt
     2401 tatttctttt ggttctttcc tctccacttt cactgtttga cgaaggacat ctagttcttg
     2461 caagagcgtt tcgtattgtt cttgggtcag catcacgcca gctacttttt cacgattaaa
     2521 tatatatacg ccagttcccg cgtctctcgc ttgagcaaat acatccatcg gcgatcgttt
     2581 cacttctgtg atcgaagatg tgggaacttc taatttcttt aattccaatg gatttcctcc
     2641 tttactaatc gctttttatt atagcacaga ctgacttgtt ttgatctttt ttccaaaggt
     2701 tttatcggtt tttgcctaac cagtaattga tatcggtaag aagtctttcg ataccaaatg
     2761 aaggatggta cagatacgtt ttttgatctt cgttatggtc tagtggaaaa aaatgatcgg
     2821 tgatcactag atctgcatac cgaggcggct tatttcgagt acatacatgt atagagatcc
     2881 gttttcgatg tcttagcgtt ttgttcagta ccccagcaag tcttcttgct tcttggtgtt
     2941 catagcgaaa aaaaaagacg attttcactt caggagggtg gacatctaaa taccctcgtt
     3001 cctggagtgt tttcagtaaa agcatccccc actctactgc ggggtgtgca agtgcttgtt
     3061 ggatcgctag ccgaaattga tatagtttat ctttggaatt gggaaatttt tctaatgttt
     3121 cctgataata acgaacttct tctatcagcc ggtcatggat accaattacg taacgaagtc
     3181 attcttggta aagtactttt gtcagctttg atttgctttc gctctcaaat tcgctattca
     3241 gccaaaacag cgaatcaagc ttttcagtca caatcgtcaa tttccgatag tcttgtgtct
     3301 cttctatccg tttccaccgc ttttcagtaa tctggttaca gctcaagcgc aaaattgcta
     3361 gataattttt ttcttttatt ggcaaaatct gctcctctct aaatagcaat tcaggaaaca
     3421 attcaaagaa tagtgttcga ctgtctccta aaatctcgat ttctgccgga tcctttcttt
     3481 gcttcaataa ttggatgaac atctcagaaa aggactgctc tccatcatct gcaaaaaaat
     3541 gcggaaaatc tttcaaaaag caaaacagca gatatcttac ttttctttct gaaccaaaga
     3601 tctgtgcttt tttataagaa aaagaaaggt ggtactcgcc aaaccacttt ctcaatcgct
     3661 cttgcgtaga acgatcgtac ttctgctcgt aaggttcatt caatagcaga tttttcagta
     3721 aacaaaacgg gggtgattgg atcagaaaaa caaacaagag ataaggttcg ctatatcctt
     3781 gatcataaac taactgaacg gttttcttat gaagaatcag ctgaatggga cagtcgcttt
     3841 ccgccaaacg tttcagatgc cgatccactg ttttaacatc gattttcagt tcttcagcta
     3901 aatcagcttt tcgccagatc aaatctttca ttagattgat tagtgaaaat aaattctgtg
     3961 cattttttga taatagtttt tgccatctca tcgatcattt ctcacctgat ttctaaacat
     4021 attgtttgat caaaatgggt tcactcctaa aaagataaaa aactcccttt ctatttattt
     4081 aaatacgcaa atcttcctca ttatttt
//
LOCUS       DAHUVV010000150         4103 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.153, whole genome shotgun sequence.
ACCESSION   DAHUVV010000150 DAHUVV010000000
VERSION     DAHUVV010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.153"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..382
                     /locus_tag="NK767_002736"
     CDS             <1..382
                     /locus_tag="NK767_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HCH6695669.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
     gene            complement(776..1783)
                     /locus_tag="NK767_002737"
     CDS             complement(776..1783)
                     /locus_tag="NK767_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6695670.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            2250..3656
                     /gene="lacG"
                     /locus_tag="NK767_002738"
     CDS             2250..3656
                     /gene="lacG"
                     /locus_tag="NK767_002738"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCH6695671.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            3679..4002
                     /locus_tag="NK767_002739"
     CDS             3679..4002
                     /locus_tag="NK767_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCH6695672.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
BASE COUNT         1362 a          607 c          801 g         1333 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccttcta acaattcctg gggccatgtc gcccacagag atattcaaaa tgtttgagat
      121 gggtgctgat attgttaagg tgttccccgc caactgtctt ggaccggaat atttcaatca
      181 agtgcaagcc cctttaggaa gtttaccttt gatggctgta ggaggggtag atcagactaa
      241 tgcacaaaac tatttgaaca atggagcctc ttatgtagga attggttcaa aattttttga
      301 aaaaagtgct gttcaccaac taaattacga gaggctaatg gagttggctg aatcttttat
      361 agatagttta agagttgaat agaacccatt tgcggaatac aacttgtttt ataatatggc
      421 gttatctgta taaaacatag tatcacagtg aatgagattg aactggtaga ttgatttttc
      481 gaccagttct ttttgcgcgc tctgatgctt gatatattcc caacgagata aaaaatcccc
      541 caagatacta agatgaagcg tttatccaac ctcatactta gataaaaagg aggaaatgtt
      601 ctgaatgata aacaaaaatt agcgcgcaca atttggcgat gaaaaaatca tattgtattt
      661 acgtcaaaat atcaccatca aattatttat ggaaaacata ataggcataa acccgattgc
      721 tcggctacta tgtggacaca attatagttt taaagataag tttcaattac ttattttaca
      781 cctcttcttt agggataaaa cttttcctaa cggtcaattt tgcgccgatt aatgttttct
      841 ttgatatgtc ccgcgaatat accagttgat cacttaataa atctacacag gttttagcta
      901 tctcttcgat atctatctta aaagttgtaa gtggtggtgc aacgaatttt gcgatgtcgt
      961 catcattaat acttattact gccatatctt tagggatgtt aatacccaat tcatggcagc
     1021 cctgaagaac gccggcagct atagtgtcag atggtatgaa acatgcctcg ggcatatctt
     1081 catttaactc agctttcatt ttaagagtta attgataccc ttcgtcaaca gtaaatgatc
     1141 cttgggagaa aatatacttt tcattgagta gtccgctctt ttctaaatga tatcggaagg
     1201 catattcacg gctatcaatc tcttctttac ccgtttctag gttaaagaaa tttcctccaa
     1261 taaatccaat ttttgtgaaa ccttcattaa taaagtagtt aattgcttgt ctggccattt
     1321 cgtggaaatc aggttctaca gtatcgaagt gttcaggtaa tgggtttatt cctcggatta
     1381 cccccttata ccctttttga tttaagtagt acaattcctt ttttgtcaat tgtcctattg
     1441 cgataaatcc agtaatatcg tcaggaatgc tttttacacc atcgttttta gagatgaaaa
     1501 tcaaatctag attagctttt ttactataat ttttcaaatc atctttgaca tgttgatagt
     1561 agacatcctt ttttaactgt tcctctgaga tccaaaaaag aatggcaatc ttgtacagag
     1621 tagtcttaat ttttgtcaga tcatatccta aagatttggc tgtaacgatt attttgtttt
     1681 tagtatcaga tgtaatagag agtgactcat cacctttaaa aagtcttgat atagtagccg
     1741 ttgagtaacc agattttttt gcaatatctc taatagtaac cattgttcat cctcctaggg
     1801 atataataat ccttttcttt gttaaagtaa atcgaaactg ctcttttagt aacttaaatt
     1861 actttttcta gcaggaaacg aaactctgat atcagcagag agcctaatcc attaacagag
     1921 aagattgaat cgataatgga tagccacttg gagaatgatt agtaggtggg attgatttta
     1981 gtgggaatca gtccgttttc ttgtttcgtg taaattaaat tagtaaaaag ataaatttat
     2041 tttacaaaaa aacgtttaca aaacggtttt tgaatggtat atttatttta catcaaggga
     2101 tataagtgct tgtatatcgc aataaaatag atattcgagg tcggtttttc gttgtgtaaa
     2161 aaaatttact tatacatgtt aaaattatgc tgaggtgatt tatggattat tggcatattg
     2221 atcaaactaa ttttcaagga gagattaaaa tgaattataa aactttacca gaagacttta
     2281 ttatgggagg agctacggca gcttatcaag cagaaggctc tacaaaagaa ggtggaaaag
     2341 ggcaagtagc gtgggatact ttccttgtag aaaagggacg ctatactgct gatccagcta
     2401 gtgattttta taatcaatat ccagttgatg ctgaattatg caaaaaattt aaaattgata
     2461 gtattaggat gtctattgca tggagccgta tttttccaaa aggctatggg gaagtgaacc
     2521 aaacaggagt ggaatattat cataaggtat ttgctgaatt gaataagcat ggtattattc
     2581 cttttgtaac tcttcatcat tttgataccc ctaaagaact atttgataat ggagattttt
     2641 taaatcgaga aaatgttgaa catttcgtaa actatgcaca gttctgtttc gatgaatttt
     2701 ctgaggttca ttattggagt acatttaatg agatttatcc agttgcttca aatcaatatt
     2761 tgatcggatc gtttcctcca gaagttcaat ttgatataga gaagactgta acttgtttac
     2821 acaacatgat gtatgcgcac gcaaagacag taaacatttt taaagatggt ggatataaag
     2881 gggaaattgg tgttgttcac tcgctagaga caaaatatcc agcaagtgac aaagaagaag
     2941 ataagcatgc tgcgaaatta gatgatgcgt tatcaattaa gttcttatta gatgctactt
     3001 atttagggta ttactcagat gaaacaatgg cacatattaa tgagatttta gcagcaaatg
     3061 ggcaatctgc agatattgat tctaatgatt ttagagagat gaaaaaagca gcaaatcgaa
     3121 atgattttct tggtatcaat aactatggta gccattgggt taaagagtac aaaggagaaa
     3181 gcacacttca ctacaacact accggagaaa aaggtagcca agtctattgc attaaaggga
     3241 ttggcgagag aatcgtgcgt gaggatcttc cgaaaaccga ttgggattgg atcatttatc
     3301 caaaagggtt atatgattta cttgtacgaa tccaagacga atatccaaat tataataaaa
     3361 tatacattac tgaaaacggg ctagggtata aggataattt tgaagatggg attattctag
     3421 atgatgatcg aatcgattat ttacgccagc attttaatgc gatttccgat gctatcaaaa
     3481 tgggagttaa tgtaaagggg tatttcattt ggtcattaat ggatgttttc tcttgggtta
     3541 atggatacac taagcgttat ggactattct atgtagattt cgatacacaa aaaagatatc
     3601 cgaaagagtc tgcatattgg tataaatttg ttagcgaaac aaaaacgata atataaaaat
     3661 agttaggagg gcaaggaaat ggataggaaa gaattgcaat tattaggatt tgaaattgtt
     3721 gcttatgcag gagatgccag aagttcatta ttaacagtgt tgaaggaagt gagagaaggt
     3781 aattttgata atgttgaacg attattagcg gatgctgatg aaaatttaac cttagcgcat
     3841 aactcacaaa cgaaatgttt agctgaagaa gcgtcaggga acgaaatgga agtaggcgta
     3901 atttttattc atgggcaaga ccatttaatg acaacgattc tattaagaga acttatcaat
     3961 gattttgttg aattgtacaa agaaaaattt gctaaagcat aggagaagaa aaatgaatac
     4021 gattattaag caaattgaga agggaaaacc gttttttgaa aaaatctcac gtaatattta
     4081 tttaggggca attcgcgatg gct
//
LOCUS       DAHUVV010000151         4092 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.154, whole genome shotgun sequence.
ACCESSION   DAHUVV010000151 DAHUVV010000000
VERSION     DAHUVV010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.154"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..153)
                     /locus_tag="NK767_002740"
     CDS             complement(<1..153)
                     /locus_tag="NK767_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCH6695673.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="NK767_002741"
     CDS             complement(475..696)
                     /locus_tag="NK767_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695674.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="NK767_002742"
     CDS             complement(794..1039)
                     /locus_tag="NK767_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695675.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1279..1893
                     /locus_tag="NK767_002743"
                     /pseudo
     CDS             1279..1893
                     /locus_tag="NK767_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2263..3264)
                     /locus_tag="NK767_002744"
     CDS             complement(2263..3264)
                     /locus_tag="NK767_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HCH6695676.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3423..3689)
                     /locus_tag="NK767_002745"
     CDS             complement(3423..3689)
                     /locus_tag="NK767_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6695677.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3679..4035)
                     /locus_tag="NK767_002746"
     CDS             complement(3679..4035)
                     /locus_tag="NK767_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCH6695678.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1296 a          744 c          541 g         1511 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     1201 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     1261 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     1321 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     1381 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     1441 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     1501 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     1561 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     1621 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     1681 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     1741 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     1801 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     1861 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     1921 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     1981 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     2041 aaggcattta acattgacaa cttagtataa tatatcttat gtaaactaat aagatttgaa
     2101 ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat ttacgattgc
     2161 acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca ttaaaaaagt
     2221 ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg gtaaatctga
     2281 ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct ccataataac
     2341 agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt cctctgaaat
     2401 caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct ttaaaattcc
     2461 cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac tccttaaatt
     2521 gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg aaaagataga
     2581 attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa attctattgg
     2641 atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt ttttgttaat
     2701 tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt ctaaattatt
     2761 ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga tattgtcttt
     2821 gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct taacttctat
     2881 atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc ctacctcgcc
     2941 tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat aattatcaac
     3001 tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt ctacaatttg
     3061 tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg cattcgtttc
     3121 ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat tccatctata
     3181 tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg ctataaatac
     3241 ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc tttaaagtaa
     3301 tttttgtata aaatttattg acttttacac tataggacaa cttttatcta tttagtacaa
     3361 aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt ttcaactcgc
     3421 ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc atcaagttta
     3481 tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt tgtatgtgct
     3541 ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata aagagcaacc
     3601 gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt gtctttttgc
     3661 ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa gttcttttgc
     3721 atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat gggtaatgac
     3781 ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc ctaccttact
     3841 tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt gagctactag
     3901 catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat ctttttttgg
     3961 taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa atgtttcaaa
     4021 agatattttt tccataccca attatatcat atattgattt tatattatag tatataacat
     4081 aaaaattcga at
//
LOCUS       DAHUVV010000152         4048 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.155, whole genome shotgun sequence.
ACCESSION   DAHUVV010000152 DAHUVV010000000
VERSION     DAHUVV010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.155"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(158..1663)
                     /locus_tag="NK767_002747"
     CDS             complement(158..1663)
                     /locus_tag="NK767_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCH6695679.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1660..2346)
                     /locus_tag="NK767_002748"
     CDS             complement(1660..2346)
                     /locus_tag="NK767_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6695680.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2539..3960)
                     /gene="gndA"
                     /locus_tag="NK767_002749"
     CDS             complement(2539..3960)
                     /gene="gndA"
                     /locus_tag="NK767_002749"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCH6695681.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1116 a          857 c          692 g         1383 t
ORIGIN      
        1 tcgttcatct aaaagatact tacatttaat taacggatgt tattctattt aattgttcgc
       61 tagttttttt ataatgacat ccctgaattt ctcacttgct tgtattttgt accttattct
      121 ttgttggata tataaaaaaa caagtaaagc acatgcttta cttgtcttct gcctttgcga
      181 taggaataaa gattctaaaa attgtccctt gccctactac actttctgct aagatacgtc
      241 ctttatagtt ttcaaccagt tgtttagcga tagacaaccc cagaccattt ccacccttcg
      301 ttctagctct tgcattatcc actcgataaa aacgattgaa gatcttatta aggtcttctt
      361 tgggtattcc ttcgccaaag tcctgaatcg cgatctcaaa ttcattcaga gtggaagaaa
      421 tagagatatg gacttctttt cgtgtagttg aatactttac tgcgttatcc aatatgataa
      481 tgattaattg ttcaaagtga ttacgataaa tttgcagggt cacttctttt gtcaaatcat
      541 catctaaagt gatcacatag tctggatata atatcttgaa attattataa acttgatagg
      601 tgacttcttt tcctttagtt gtttcattcg cataatgaac atctacttgt tctgctcttg
      661 ataagtctag catctcttga actaagcttt tcatacggct gatttcttgc atgctggcgc
      721 ttaatgattc ttctaatatc tcaggatcat ctttccccca acgatttagc agactaagat
      781 gcccttcaat aattgcaaca ggtgtcctta actcatgaga tacgtcttcc acgaattgct
      841 cttgttgttc aatatacaaa cgcatacggt caagcatctc attgaatatt tcagctaaat
      901 ctgccaattc atcatttgtg tcgatttcag gcatgtggat atctgattgc ggatctttac
      961 gtatagtatc cattgtatct ctgagtacct tgagtggttt taagaaataa gaagataaaa
     1021 agaaacctaa aatactactt aagatcaaag aaacaacttc taaaacaata agagtcaata
     1081 atagcttatt tcgaatatca tagaaagaag ataattcata gaagctttgc gcatagccga
     1141 tcttttctct tgtaccttta gaatatatag gctggataga taaaaagcca gtttttcctt
     1201 ctagtgtaac gatcgttgga actttacgtg tagtctgaat caggtttcga ctgttttcat
     1261 gggttttaaa aatcaagttc tcgtccaaat cataaacata aagatccatt gatggctgcc
     1321 ctagctcagc tataaaggaa tcgatcttca ttaagtttcc ttcgaccagt gaagtatgat
     1381 ctacgacctc atctccgtca tttgtcgctt tcgtcagatt acgatacgta tttactaatg
     1441 tcagttcttc atccgagtta gccaaacgag aagctacttc tgaaatcgta cgctctacat
     1501 tgtttctttc ttttgccaca atcagattga tagatgattt ataggtaatg acagcgaaaa
     1561 ttgtaaacac aacgaatatg aagaaagaac tcgcaaaggc ccacttgatt gtcaaggagg
     1621 gccctttcag ttctcttttt tccgattttt tcggctttat cacgagcgca tcacataacc
     1681 tgttccgcgt acagtttgga tatagctttc ctccccggga acgtcaattt tgttgcgaag
     1741 ataacgaata tatacgtcca ctacgtttgt ttcaacttct gtttcgtatc cccacacttt
     1801 atttagtaaa acgtcacgtg ctaataccac gttcacgttt tccattaaag taagcagcaa
     1861 ctcgtattct ctttttgtta gttcaattac ttcatttccg cgacgtacga cacggttttc
     1921 tttttcgata gtcaaatcgc gataagtaat agtagtttgt ttagccacat ttttatcgcc
     1981 ttcaatatcg attcgacgca acaatgcacg aagacgtgct aataattctt cgatagcaaa
     2041 tggtttgacg atgtagtcat ctgctccgtg gtcaagtcca gatacacgat cgatcacaga
     2101 atctcttgca gtcatcataa tgattggtgt atttttgact tggcgtacac gtctgcagac
     2161 ttctaaaccg ttcaattctg ggagcatcag atccaacaat atggcatccc actcattatt
     2221 taatgccgca tctaaaccag ttcggccgtt gtagtggact tctgcgttat acccttcatg
     2281 cttcaactct agctcgacaa atcgtgccaa gttcttttca tcttcaatga ttagaatatt
     2341 gctcatttat tattttatcc tttctattct actggctttt taaaaactct atcattatac
     2401 cgataatatg cttaaaaatc tatagtcatt ttatcatgga tagcaatttt aaaaaagagc
     2461 tgaagtctac tcataacaat cgacttcagc tctttcgtcc tctttttatt ttctcatttc
     2521 ctaacagccg aaagactatt attcttcatg gtaccatgag taatggaaga ttccttcttt
     2581 gtcgacacgc tcataggtat gtgcgccaaa gtaatcgcgt tgggcttgga ttatatttgc
     2641 aggcagacgt tctgctctgt atgaatcgaa gtaagcgatg gctgatgaga aagtcggtac
     2701 aggtacgcca gcttggacag ccaaggcaac gacgtctctg actgattgct gatatttttt
     2761 cgtgatttca acgaagtaat catctagcaa taaattttcg atctcaggat ttttgtcata
     2821 ggcatccgta attttttgca agaatcgagc tcggatgata catccagcgc gccagatttt
     2881 tgcgatttca ccaaatggca agtcccaatc aaattctttt gaagcgacac gtaattgagc
     2941 aaatccttgg gcataactca tgattttact gaaatacaac gcttctcgga ttttttcgac
     3001 caattctttt ttgtctcccg tgaactcaaa atcgttcgta cgagacaaaa tcttgcttgc
     3061 ttgtacgcgt tcttctttat aagcagaaat atagcgggca aatacagatt cagtgatcaa
     3121 tggaagtggc acacctaagt caagcgcgct ttggcttgtc catttacctg ttcctttatt
     3181 cccagctgca tcaaggatca catctactac tggtttacct gttccttcat cgtctttacg
     3241 tgtcagaatg tcagcagtga tttctatcaa atagctgtct aattcgcctt gattccattc
     3301 tttaaagata tcagccattt cttctactga aaggtttaaa atattcttca ttaagtcata
     3361 agattcagca atcagttgca tatctccata ctcaataccg ttgtgtacca ttttcacata
     3421 atgtccggct ccatttgggc caatatatgt gacacatggt gctccgtctt ctgcttttgc
     3481 agaaatttgc tctaagattg gtgcaactag cgcataggct tctttttgtc caccaggcat
     3541 gattgaaggt ccttttaaag ctccttcttc accaccggaa acgcctgtac cgataaagtt
     3601 gatgccagag ttggctaact cttcattacg acgcatcgta tctttaaaga atgtatttcc
     3661 tccgtcaatc aaaatatcgc ctttatcaag gtgaggaagt aactcttgga ttgttgcatc
     3721 tgttgccggt cctgctttga ccattagcaa gatccgacgt ggtttctcga tagattctac
     3781 aaattcttcg attgtatatg ttgctttgaa atttttatct gggtgttctt ctactacagc
     3841 ggttgtttta gcccctgtac gattgaataa agccactgaa tagccgcggc tttctatgtt
     3901 caaggctaga tttttaccca taacggccat accgacaacg ccgatttgtt gttttgacat
     3961 atgaactcct cctacatacg ggatttctcc ctaatctctt acattatagc ttaacacggc
     4021 tacattgcaa actaaagaac aacatatt
//
LOCUS       DAHUVV010000153         4039 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.156, whole genome shotgun sequence.
ACCESSION   DAHUVV010000153 DAHUVV010000000
VERSION     DAHUVV010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.156"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            179..775
                     /locus_tag="NK767_002750"
     CDS             179..775
                     /locus_tag="NK767_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCH6695682.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="NK767_002751"
     CDS             899..2698
                     /locus_tag="NK767_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCH6695683.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="NK767_002752"
     CDS             complement(2976..3188)
                     /locus_tag="NK767_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695684.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3650..3799
                     /locus_tag="NK767_002753"
     CDS             3650..3799
                     /locus_tag="NK767_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695685.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1187 a          689 c          712 g         1451 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaata
//
LOCUS       DAHUVV010000154         3914 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.158, whole genome shotgun sequence.
ACCESSION   DAHUVV010000154 DAHUVV010000000
VERSION     DAHUVV010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.158"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..1530)
                     /locus_tag="NK767_002754"
     CDS             complement(<1..1530)
                     /locus_tag="NK767_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695686.1"
                     /translation="MKIIKKKIAGRKINWLALSLLALMFIVIFTGNVAHASIFNWFDD
                     TESQTEFLNNPVYQPYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFVESAGLNKVYQSVLSTIVVGLMILSLVIIGYKMVTGKGTVDLKSVGINVLMSIVLI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     MIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENSTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISIGLIALAIAYVFSAFVIITAILELAFKRV
                     LGVLVFATDLETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRA
                     KGSGNGNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMAS
                     ER"
     gene            complement(1517..1735)
                     /locus_tag="NK767_002755"
     CDS             complement(1517..1735)
                     /locus_tag="NK767_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695687.1"
                     /translation="MIENRLIDQICCLDQNVDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQEMAQKLSESLKKWAEGGNNENN"
     gene            complement(1728..2693)
                     /locus_tag="NK767_002756"
     CDS             complement(1728..2693)
                     /locus_tag="NK767_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HCH6695688.1"
                     /translation="MKTLFDYILTLTGGSVEFMAEKRMLSKTFLNNASFMKMSDSAKL
                     LYVYLNVNADDDGIVEAYPYVRMLGTPDDDVKILLAKEFLYMLNEDLLVFLPHWLQNQ
                     TLRTTRYTPSIYRFKLLNSNPDFFTRLKQISIEKILKAGQTEMLRNDLIEKWYPVGLP
                     KDDHGHAQVRLGQVSSDQSRSGKVSLGEIREDQSINLSDDMEVEIMRQKIKNQIGYNA
                     LKSNPCYKRIVEDIVNIIQSIYSEKSKTYRINREEKSANEVIEKVEQLNQFDIESAID
                     SFNAIDHEIHNLNLYWITTLYNCKNTVSSRAYNNFVTQTREVLYD"
     gene            complement(2883..3167)
                     /locus_tag="NK767_002757"
     CDS             complement(2883..3167)
                     /locus_tag="NK767_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6695689.1"
                     /translation="MDVSSVQVILPPDLDQSLRKYFYQVAKEAVDQVRIETSLSKEIF
                     RLGEACEYLSCSRSTLKKFINQRGLKVSIIGDCQFIRKEELARFIKEYEI"
     gene            complement(3180..3473)
                     /locus_tag="NK767_002758"
     CDS             complement(3180..3473)
                     /locus_tag="NK767_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6695690.1"
                     /translation="MTDNEINAILSLVKTKKDEVVRLDSPVRKYRRFCGYRQKELAEL
                     LEISELTYRNKEKGRVPFKDYEIERIVKLFHKDGIHLDIVDIFFSDLKTKKDE"
     gene            3542..>3914
                     /locus_tag="NK767_002759"
     CDS             3542..>3914
                     /locus_tag="NK767_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6695691.1"
                     /translation="MNLNKTSVGNRIRLVRKELGLSMKEFGKKITPTAADSIVSRWER
                     GISLPNNDRLTQIAKIAHKDINWLLWGTFKEFISSLLDKEGYTNFLNDFPETVDTIYN
                     DLMPISENLQFPDYENSILRLF"
BASE COUNT         1215 a          781 c          535 g         1383 t
ORIGIN      
        1 tctttcacta gccattgtag ctccatctga agctaaacct gataatccac gttcatgggc
       61 atatcccaaa gctcttccag ttttgccagc tttttttgat agagtttcgc cgaaattctt
      121 ttctggtttt cgatcttctc cttctccgct gtctgatcca tttccatttc cgcttccttt
      181 agcacgatta aatccatttg atccttttct aaacagcatt gcgcccatac caaaagcaga
      241 agcaagttgc ccataacctt ctttgagtcc aatatcaaca ccaaagtatc tcatgactgt
      301 tccactgcct ttgattaaaa caaaaacggc acagatataa gcaattgttt ttataaaaat
      361 atttgttgag ataccttgtc ctgagttcag aaaattgata aacatagcaa acatggatag
      421 tccaaatcct tgaaagccta cagtcaaata gcactgtaga atatctgata gaacaacttt
      481 tgaacgctgt cctgtttcta agtcagtagc aaatactaaa actccgagta cacgtttaaa
      541 tgctaactcg agtattgcag taataatcac aaaggcgcta aaaacatagg ctattgctaa
      601 agctatcagc ccaattgaaa tgccccatag attcgcttga tagcgataat atcctgattt
      661 gataccatca acaaaggttg acaaaaaatt atctgaaaat ttagtagcaa caaattcatt
      721 atcagaattt tcaactaatt cataacgcag gttatcagct gttgagttct ctttttccat
      781 tttatcaatt acattatctg tcaatacttg cgtaagatcc gttttccaaa aattttcttt
      841 tgttagattg ttccgatcat tcgttttatc gatcatggaa tattgatccg ttttattgat
      901 ataagctaaa tcagtaaccc cttgttcaat cagcgaccat gccacaccgc catcttgatt
      961 gttaatacta gttgcgtcat tataaagagt ctttgcaaac attattcctg aaagtttttc
     1021 tgttgatcca atcaaagtcg gcattaaaag aattaatact atcgacatta aaacattgat
     1081 tccaacagat tttagatcaa ccgttccttt tccagtcacc attttataac caataatcac
     1141 tagtgaaagt atcattaagc caacaacaat agtgcttaaa actgactgat agactttgtt
     1201 caatccagca ctttccacaa agctaaacaa atctaacact tcaggaatca tattttgaat
     1261 accatctaca attgcaaaca tcgattttat aacagaccac cctatccaca atccaaattg
     1321 actgtaaaaa tttccgttat atttttttaa atacggttga tagacaggat tattcaaaaa
     1381 ttcagtttga ctttccgtat catcgaacca attaaaaatg cttgcatgag caacattacc
     1441 ggtgaaaata acgataaaca ttaaagcaag taagctcaga gctaaccagt ttattttcct
     1501 accagcaatc ttttttttaa ttattttcat tgttacctcc ctcagcccat ttttttaggc
     1561 tctcagaaag cttctgagcc atttcttgat taattatgat atgagttaca caattgtcat
     1621 tttcagctct taaatcgaga accacggtgt taaagcagat actctcgatt aattcgagag
     1681 tatctacatt ttgatcgaga cagcagattt ggtctattag acgattttca atcatataac
     1741 acctctcttg tttgagtaac aaagttatta tatgctctgg aacttacagt atttttgcag
     1801 ttatataaag ttgtaatcca gtaaagattg agattgtgta tctcatgatc aattgcgtta
     1861 aatgaatcaa tagcagattc aatatcaaat tgattgagtt gttctacttt ttctatcact
     1921 tcatttgcag atttttcttc tctatttatc ctgtatgttt tgctcttttc agagtatatt
     1981 gattgaatta tattaacaat atcttcaaca attcttttgt aacaaggatt ggattttagc
     2041 gcattatatc caatttgatt ctttattttt tgtctcataa tttcgacttc catgtcatcc
     2101 gatagattga tagattgatc ttccctaatc tcacctaaac taaccttacc tgacctactc
     2161 tgatctgaac taacctgacc taatctaacc tgggcatgcc catggtcgtc ctttggtaga
     2221 ccaacgggat accatttttc aatcaaatca tttcgaagca tttccgtttg acctgctttt
     2281 aaaatttttt caatactaat ttgttttaat cttgtaaaaa agtctggatt actatttaat
     2341 aatttaaatc gatatataga gggtgtatat cttgtagttc ttaacgtttg attttgcagc
     2401 caatggggaa gaaatactaa taaatcttca ttcagcatat agagaaattc tttagctaaa
     2461 agaattttca cgtcatcatc aggggttcca agcatccgta catatggata tgcttcgaca
     2521 atcccatcat cgtctgcgtt tacattcaga tatacatata ataattttgc tgaatccgac
     2581 atcttcataa aactcgcatt atttaaaaaa gtttttgaca acatacgttt ttctgccata
     2641 aattccacac ttcctcctgt caatgttaaa atgtagtcaa ataaagtttt cacagaactt
     2701 tattattgag tgttcttccg gtctgcaaac tgtaaggagc actctttttt tgtatattca
     2761 ccttttatta aatcaaatag aaaataaatc ttgatttctt ttaaactcaa ctttgcttct
     2821 tcatcatcgt tcaaacaaac acttcctttt tttcatatca aaagcacacc acgcaacttt
     2881 ttttatattt catattcttt gataaatctt gctaactctt cttttctgat aaattgacag
     2941 tctccgatga tactcacctt taaaccacgc tgattaatga atttttttag tgtacttcta
     3001 ctacaactca aatattcaca cgcttctcca agccggaaaa tttctttgct taaagatgtc
     3061 tcaatacgaa cttgatcaac agcttcctta gcaacttgat aaaaatattt acgtaatgat
     3121 tgatcaagat ctggcggtag aataacttgt acactcgata catccatgat gcccactcct
     3181 cattcgtctt ttttcgtctt caaatcacta aaaaaaatat ccacaatatc taaatgaatt
     3241 ccatctttat gaaatagctt tacgattctt tctatttcat aatctttaaa cggcaccctt
     3301 cctttctctt tgttacgata cgttagttcc gatatttcta ataactccgc aagttctttt
     3361 tgtctatatc cacagaacct gcggtatttt ctaactggag agtccaacct taccacctcg
     3421 tcttttttcg tctttaccaa cgatagaata gcatttattt cattgtccgt caatacttac
     3481 ccgtcgtttt tcgtagattc cattccgttt ttgtgttaca ttactgtcgg agggatataa
     3541 tatgaacctt aataagacat cagtaggaaa ccgtattcgt ttagttcgta aagaactcgg
     3601 gctttccatg aaagagttcg gaaaaaagat aactcctacc gccgcagata gtattgtttc
     3661 gcgttgggag cgagggatta gcttacctaa taacgacagg ctaactcaaa ttgctaaaat
     3721 tgctcataaa gatattaact ggttattatg gggaactttt aaagaattta tttcatcatt
     3781 gttagataaa gaaggataca caaatttttt gaatgatttt cctgaaacag tagatactat
     3841 ttataatgat ttaatgccta tttcggaaaa tttacagttt cctgattacg aaaattctat
     3901 tttacgacta ttca
//
LOCUS       DAHUVV010000155         3701 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.159, whole genome shotgun sequence.
ACCESSION   DAHUVV010000155 DAHUVV010000000
VERSION     DAHUVV010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.159"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..1012)
                     /locus_tag="NK767_002760"
     CDS             complement(<1..1012)
                     /locus_tag="NK767_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HCH6695692.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIE"
     gene            complement(1016..1426)
                     /locus_tag="NK767_002761"
     CDS             complement(1016..1426)
                     /locus_tag="NK767_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCH6695693.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(1444..1935)
                     /locus_tag="NK767_002762"
     CDS             complement(1444..1935)
                     /locus_tag="NK767_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6695694.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(1945..2736)
                     /locus_tag="NK767_002763"
     CDS             complement(1945..2736)
                     /locus_tag="NK767_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCH6695695.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(2729..3502)
                     /locus_tag="NK767_002764"
     CDS             complement(2729..3502)
                     /locus_tag="NK767_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6695696.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1146 a          778 c          503 g         1274 t
ORIGIN      
        1 cttcgatttt aggaacaatt actctttctt tttttccaaa ttctttctct aacttaaaac
       61 ttgaaaatgg agtgatttct ttaaaattct caattatttt ttcaggcagt ttaacaccaa
      121 gagttttgtc tgtcttttga ataatttgat gtggaacaag tgcaccgccc caatcccagt
      181 tattttgatc gttctcccct tcttttgttg ccacccagcc agttagaaaa cggccagttt
      241 cattagttcc gactgttctt gctgcataat aggcttttcc gtcgaaagct tcgtctactg
      301 gtgatttcca atctgaaaat aatcctgctc cgactcgata tttagtcttt ttatcttcac
      361 aatactctga aaaaagtaaa taccacgttc catcaatttc aaatatatca ggcatttcaa
      421 tcatgttaaa ttcattatta gcccagtagt ctcctttgaa attccaatct tttaaatctt
      481 cggattcaaa atagaccaat ctccccgtcg gctttttctt acttgctggt tttctagctc
      541 ctagtatcaa aacgtatttt ccatgatctt gatcaaaaat tgcaaaagga tcgcgccaat
      601 cattcttatc atatccttct tgaggaacaa gttttgctgc ttcgccatcc tttttccaat
      661 gaattaaatc tttagaactt gcaatcatta acacttctga agtttttcca tccaacttag
      721 ctttgcgatt atgtccagta tacaaagcta aatactcatt gattccttgg acaatacttc
      781 cagcgtaaat gtattgatca atgtcatcgt tgccaccacg atgaattact tccccgtatt
      841 cttcaaattt cacaaaatca gtggttgtaa ctagtgacca cccaaaagga tcagttaaag
      901 gtttaggatt tctcgtatct cgttgatgaa atagataaaa agttccatct ttataaaaag
      961 gcatgcaatc cccaaaccaa aaatctttag gcttgtaaaa aattttttgc atttatcact
     1021 catcctcttc gttagtatgt ctttgcatat ctgcaagaca cgcaattgta ttcatatttc
     1081 ttagattttc gatgatatca tcgctttttt ttccttgtaa tctcaaattg acagcctcaa
     1141 tcaaaattcc taaattcatg ccaccaagaa gttgaaaccc attttgaaat acttcacttc
     1201 ttttagcaaa ttcgttgaaa ggtgttccac caatcaagtc agcaaatacc ataacttctt
     1261 gattttttac ttgagaaact attttttcaa tgttttcacg gaattcttca atgcctgcat
     1321 ctccaagtgt taccacatga caatttaaat tatcgccaaa aaacattttt aatgtatcta
     1381 caaatccaat ggcaatattt ccatgagatg taattataaa ctccatccac tcatcctctg
     1441 ttcttaataa ataaaatcct ttaaaaattc ttttgaatct ctaggagttt gtatcactgt
     1501 cacttcaact ccatcactaa ttagttcttt aaaagcctct ctctcttctt cagtaatatt
     1561 tatcgtcgga cggatgcgtt ctgttccatt ccttcttgat aaatttccta cgtttacact
     1621 tgttattggt aaaccgttat caatcaaaaa tttaacatcg attggagaaa ctgcaacaat
     1681 aagaactttt tgtccttcat atttaccaga aagaatattt ttaactgcta cttcccttgt
     1741 aattattgaa gttctaacat taggtggtgc agcgaggcga actacagctt tttttacgtc
     1801 atcattggct acttcattat taataaccat aattctgtta gcactcaatt gatttgtcca
     1861 ttgtgttgct acttgtccat gaatcattcg atcatcaata cgaacatgta taataccttt
     1921 cattttttca tccatattca acacctactt caaaatattt aacgctctta acgcaataga
     1981 aactactaag attaatagaa ttacataaac tggtttaacc tttcgtccta acaaataata
     2041 cacaattgct actacacatg caggtaataa accaggcata acttgatcaa agatttcatt
     2101 tccggtcatt gttaagtcgc cttgtttaaa ttcaaagcca acattcactt ttacaactgt
     2161 ggcaatgagt gcaccgacca ccattaaccc taaaattgat actgcatcag taatagactt
     2221 taattttgtt tcaatttgag ttagtaggct cgcacccgat tctttcccag caaggtataa
     2281 aggataagca gtaatcctta ataaaattga tgccagtatc cacaaaataa ttccaacaaa
     2341 actaccttcc aatgccatat tagctgcaat tgctccaaaa atagtccatg gaataactac
     2401 aaatacacta tctccaattc ccgctaaagg acccattaga cctgttttca gagcacttac
     2461 tgcttccaaa gacttatttt tttcttcttt ttcaattcca acattcatac caataatgta
     2521 aggcgctgta tacggcgtag tgttaaaaaa ttgtaggtga gctaaaacgg attgtttcat
     2581 tccttcagga tcatctttat atgtgtcctt gataaaagga agcatagaat acgcataacc
     2641 caacccttcc attttttcat aatttaatga agttgcacca ttgagaatcc accggaaaat
     2701 tacttgtttg tcgatttttg tcagtttctt attcatcttc gtcaaaacct cctgttgcag
     2761 ctggtgctac ttgagtatta tttgatctat ttttatatac gattagtgcg attgcagcgc
     2821 caagtaacga aatccctaaa attggtattt ggaaataaac tgccaaaacg aaaccaacta
     2881 acaaataggc aaagtaatct tttgtaggta aatatttaag tagcattcct atcccaacag
     2941 caggtaatag tccccctgct acctttaaac caccggtaac ccattcagga atataattga
     3001 ttgcacttcc cactaacgtt gggcctaata tcacagttaa caaaacagga attccagata
     3061 caaagcttgt attaattgat gccaaatatt gcatccactc taatttttga taatcttctt
     3121 tttgtgcgaa tttttctgcc ttttgttgaa aaaaaatgtt tgttgaccac tttagaacat
     3181 ctagttgtac aattaacatt gctaccggta ttgctaaagt tattcctata gaagcatcct
     3241 ggttagttga gattgctaaa taagtaccga tcaatgctgc tgtttgataa tccggtactg
     3301 atgcaccacc aaaactagta atacctagtg tcattaattg caaagtccca ccgatataaa
     3361 gtccagtttg aatatctccc ataataagtc cggtcaagaa acctgcaacg acaggttggg
     3421 aggttccgat tttgggccct tccttatccc agttaatgaa aaatccatac aacacaacta
     3481 aaaatgtttg ccaaaacgcc attttgttct ctcctttctt cttatacttt tatataagca
     3541 attactgtgc caactaacat tatccaataa agcgggaaaa tacagtaaat attaattaaa
     3601 tgagcgtcta aatatacttt ggacaccctt tcattttttt agacgctagt atgctattta
     3661 gaaaatagtc tgctcaaaaa ttgtatagat taactcatac t
//
LOCUS       DAHUVV010000156         3663 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.160, whole genome shotgun sequence.
ACCESSION   DAHUVV010000156 DAHUVV010000000
VERSION     DAHUVV010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.160"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..276
                     /locus_tag="NK767_002765"
     CDS             <1..276
                     /locus_tag="NK767_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HCH6695697.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="NK767_002766"
     CDS             419..640
                     /locus_tag="NK767_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6695698.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="NK767_002767"
     CDS             656..1498
                     /locus_tag="NK767_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695699.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="NK767_002768"
     CDS             1561..3363
                     /locus_tag="NK767_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCH6695700.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3422..>3663)
                     /locus_tag="NK767_002769"
     CDS             complement(3422..>3663)
                     /locus_tag="NK767_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCH6695701.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1306 a          519 c          746 g         1092 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaataga tacccgtggt gctagttaat
     3421 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
     3481 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
     3541 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtccca actgtttttc
     3601 tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg
     3661 gaa
//
LOCUS       DAHUVV010000157         3617 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.161, whole genome shotgun sequence.
ACCESSION   DAHUVV010000157 DAHUVV010000000
VERSION     DAHUVV010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.161"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            574..>3617
                     /locus_tag="NK767_002770"
     CDS             574..>3617
                     /locus_tag="NK767_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297336.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HCH6695702.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVT
                     FTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEF
                     VSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVS
                     AAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT         1344 a          556 c          733 g          984 t
ORIGIN      
        1 attataaaga aaaagaatca ataatttaat gaggctgtga caaatgtcat tttttgtttc
       61 gctaaaaacg agtgaaaaaa tgttgataac tccaatcatt cgtttaagat tctaggaatc
      121 attcagaaat gctgtgaaac cgatacttta taattataaa gaaggtgatt tcaaattccg
      181 aagaaggaat ttgaagtcac ctttttactt atgtcacagt ctcattcagt aatgcgggat
      241 aaggctcctt attccgttat ttgaatactc tgaagtttaa tctaagaaag ggtaatattt
      301 tgaaaacatg cttcatcgca tagaaatgaa cttaatttca gaaagaatac atacatatct
      361 ttttatatgt tttcagctgc tgaatggtta aaaagcacaa atatccgaaa aaaacatgaa
      421 gaaatgtact ttttttgttg taatgttcat gaaattctct aagtaaatta gttaacataa
      481 ggttagtaag attcctattc tgtgaataat acgtatcaga tttatctttt attcttacta
      541 ggaaagtaat tattaaggga gttgatttga ataatggtta gcaagaataa taagagagta
      601 tttctagaaa aaacaaagaa acgagtattg aagtacagta tcaaaaaatt aagtgttggg
      661 gttgcatctg ttttgattgg tgttggtctt gtcttgggaa caactgaact agttaaagca
      721 caagatgaaa tttctccaag cagcccactt gaaacagcga taagctccgt acaagtaggt
      781 gacaaagtag cttcggggaa tacttttcag gagaatcctg gttatacgaa aaattataac
      841 ttttcggatt tacaatttag tcctcaagaa ctaactggag acacactaaa aggcaataca
      901 attggatttg aagtttacgg aaaacataat attgcagcat caactaaaaa ctgggaaatc
      961 cgtcttcaat tagatgaacg attagctaaa tatgtagaaa aaattcaggt cgatccaaag
     1021 aaaggaattg gatcttctag acggaccttt gtaagaatta atgattcgct gggtagacct
     1081 acaaatattt ggaaggtcaa ttatattcgt gcaagtgatg gattatttgc tggggcagaa
     1141 acaaccgata cgcaaaccgc tcctaatggg gtaattacgt ttgaaaagag cctagatgaa
     1201 atttttaaag agataggcat agacaatctc aagaccgatc gtttaatgta tcgaatctac
     1261 ttggtaagtc atcaagacga tgataaaatt gtaccgggta ttgatagtac gggctacttt
     1321 ttaacagatt cagatgattt ctataatagc ttagatgtat ctgaaaataa tcctgaccag
     1381 tttaaacatg gatcagtaag tgcaaaatat gagaaaccta atactcaaac aaaagatggt
     1441 tcaggatcca caggtgctaa tggagctata atcttagatc ataagttaac gaaaaattat
     1501 aatttctcat attcatcttc cgcaaagggc acgccttggt atgctaatta caaaattgat
     1561 gaacgattag ttccatatgt agcgggtata cagatgcaca tggttcaggc tgacaaagta
     1621 acatatgatg tttcgtttga atccggaaca aaagtagctg atttagcaat cgagcgacgg
     1681 gaaggtcatg aaaattatgg cataggttca ataactaata atgatttaac taaacttatc
     1741 gattttgcta atgcaagtcc acgtccagtc gttattcgat atgttttaca attgactaag
     1801 ccattagatg aaatcttaga agatatgaaa gcaacagcac aagttgaaga aaataaacca
     1861 tttggtgaag atttcatctt tgattcttgg ttgtcggata cgaataaaaa attaatccag
     1921 aacacttatg gaacaggtta ttattatttg caagatattg atggtgatgg aaaccctgac
     1981 gataaagaag agagcggaga cacgaatcca tatatcggga aacctgaatt agaagaagta
     2041 tatgatgttg acacaacagt taaggggaaa gtattcatcc acgagttagc gggaacaggt
     2101 cacaaagccc aacttgttga taaagaaggt actgtattag cagaaaaaac tatcgctcca
     2161 aatgaaaaag atggggctcc aatttcagat actgtagaat ttgaatttac gggtgtagat
     2221 tcaagtaaat taatcgcgaa agatgaatta aaaatccaaa tcgtttctcc aggttttgat
     2281 aaaccagaag aaggttcaac cgttattaag gaatcaccaa aagcggttga taaacaaacc
     2341 gtggtagttg gatttaaacc agatgctaaa gaatcaattc ggaataataa aaacttacct
     2401 gaagatgcag agtattcatg gaaaacagag cctgatactt ctaacgttac tgatagtacg
     2461 aaaggtattg taactgttaa gatcggaaat cgaactttcg acgtggatgt agagtttgct
     2521 gtaaaagctt ctcaagctat ggaaaatgat gcaacatacg tacctataac aacaactcca
     2581 gaaacgacag ttcaaagtgg aaaacctaca tttgataaac cagatgttcc tttagctaaa
     2641 gatgcctttt caatttcaga tgtctataat aagtacttcg gtaatgcaag tgttgatgca
     2701 aacactggta ttgttacatt cactccagct aaaggtgtag gagaatcgga gccgattact
     2761 ggaacaattc ctattaaaat tgtttaccaa gatggttctg taggcacgac cgatttagca
     2821 gtaactgtaa gtaaagatat ttatgaaaat ccaggagaaa acattcctgc aggctaccac
     2881 aaagtaacct tcaccgcagg ggaaggaaca agtattgaaa gtggaacaac agtctttgca
     2941 gtgaaagacg gcgtaagctt gccagaagat aaacttccag tgttgaaagc aaaagatggt
     3001 tatacagatg cgaaatggcc agaagaagca actcaaccaa ttacagcaga tgatacagaa
     3061 tttgtatcaa gtgcaacaaa actggatgat atcattgaaa atccaggaga aaacattcct
     3121 gcaggctacc acaaagtaac cttcaccgca ggagaaggaa caagtattga aagtggaaca
     3181 acagtctttg cagtgaaaga cggcgtaagc ttaccagaag ataaacttcc agtgttgaaa
     3241 gcaaaagatg gttatacaga tgcgaaatgg ccagaggaag caactcaacc aattacagca
     3301 gatgatacag aatttgtatc aagtgcaaca aaactggatg ataaatctga cgctgacaaa
     3361 tataatcctg aaggtcaaaa agtgactaca gaattgaata aggaaccaga cgcatttgag
     3421 ggaattaaga acaaagaaga tttaccaaaa gatactaagt atacttggaa agaaaaagta
     3481 gatgttagcg cagctggaaa taaaaaaggg actgttgtag tgacatatcc agatggatca
     3541 tctgatgaag ttgaagtaga tgttacagtg acagacaatc gttctgacgc cgataaatat
     3601 gagccaacag tagaagg
//
LOCUS       DAHUVV010000158         3578 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.162, whole genome shotgun sequence.
ACCESSION   DAHUVV010000158 DAHUVV010000000
VERSION     DAHUVV010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.162"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            4..1194
                     /locus_tag="NK767_002771"
     CDS             4..1194
                     /locus_tag="NK767_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCH6695703.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            1298..2053
                     /locus_tag="NK767_002772"
     CDS             1298..2053
                     /locus_tag="NK767_002772"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCH6695704.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            2169..3467
                     /gene="eno"
                     /locus_tag="NK767_002773"
     CDS             2169..3467
                     /gene="eno"
                     /locus_tag="NK767_002773"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCH6695705.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         1244 a          677 c          753 g          904 t
ORIGIN      
        1 aaaatggcta agaaaacagt gaaagatatc gatttaaaag acaaaaaagt tcttgtccgt
       61 gttgacttca acgttccttt gaaagacggc gtgatcactg acgacactcg tatcaaagca
      121 gcattgccaa caatcaacta cgttctagaa caaggcggaa aagcaatcct tttctctcac
      181 cttggacgtg taaaaacaga agaagataaa gaaggaaaat ctttagctcc agttgcaaaa
      241 cgtctaggcg aattattagg taaagaagta acattcgtac ctgaaactcg cggagaacaa
      301 ttagaagaag caatccgcaa catgaatgac ggcgacgttg tcgtattcga aaatacacga
      361 tttgaagata ttgacggtaa aaaagaaagc aaaaatgata cagaactagg taaatactgg
      421 gcttcattag gcgatgtatt tgttaacgat gctttcggta cagctcaccg tgcacatgct
      481 tctaacgtag gtatcgcttc aactggtatc ccaacagttg ctggattctt gatggaaaaa
      541 gaaatcaagt tcatcggtga agcagtagaa gaaccaaaac gaccaatgat tgcaatcctt
      601 ggtggcgcaa aagtttctga taaaatcggc gtaatcgaaa acttgattcc taaagcagat
      661 aaaatcttga tcggtggcgg aatgacttac acattctacg aagcaaaagg aatcaagatc
      721 ggtaactctc tagtagaagc tgataaagtg gaactagcaa aagaattaat cgaaaaagca
      781 ggcgacaaac ttgtcttgcc aatcgacaat gtctgtgcac cagaattttc aaatgatgtt
      841 gaaacacaag taatcgaagg agacattcct gatggtttga tggctttaga catcggccca
      901 gcatcagtaa aattatttgc tgatacatta caaggtgcaa aaacagttgt ttggaacgga
      961 cctatgggtg tatttgaaat gagtaacttt gctaaaggta caatcggtgt ttgtgaagca
     1021 atcgctaact tagaaggcgc aacaacaatc atcggtggcg gagactcagc cgcagcagca
     1081 gaacaattag gatttgctga caaattcaca cacatctcaa caggtggcgg cgcaagcttg
     1141 gaattgcttg aaggtaaaga attaccagga ctagcagcaa tcaacgataa ataaacgacc
     1201 aagtttgctc aattctgacg agtaatcgat caaaaggtga agggagtttt catgactccc
     1261 tagccatttt tcaataaatt aaggagtgtg ttcctcaatg cgtaaaccta ttatcgcagg
     1321 taactggaaa atgaacaaaa ctgcatcaga agcaaaagaa tttgctgaag cagtaaaaaa
     1381 caaaatccct tcaaatgaca aagttgattc agtcatcgga tctcctgcat tattcttaca
     1441 agaattaatg acatcagctg aaggtacaga actaaaaatc tcagcacaaa actgctactg
     1501 ggaaaactca ggtgcgttca ctggtgaaac ttcaccagca gctcttgctg atctaggtgt
     1561 tcattatgtg atcatcggac attcagaacg tcgtgaatat ttccatgaaa cagacgaaga
     1621 catcaataaa aaagcgaaag ctatctttgc aaatggtatg acaccaatcc tatgctgtgg
     1681 cgaatcttta gaaacctatg aagcaggcaa aactgctgaa tggatcgaag gacaaattac
     1741 agctggttta gctgatcttt cagctgaaca agtgtcaaac atggttattg cttatgaacc
     1801 aatctgggca atcggtactg gtaaatcagc tgacgcacaa atcgctgacg aaatttgtgg
     1861 agttgtacgt caaacagttg aaaaacttta tggaaaagaa gtatcagaag cagttcgtat
     1921 ccaatacggc ggttctgtaa aaccagaaaa tattgctgaa tacatggcaa aagaaaatgt
     1981 tgacggtgct ttagtcggtg gcgcaagctt gcaagcagat tcattcttgt cattgttaga
     2041 cgctgtaaaa taattgttga aaatttgcga aaaatgcaag aaatgtttgc actaatgagc
     2101 aaaattaact aaaatcgtat atgagggaaa tcccttagga aaaaacaaac tcaaaggaga
     2161 gacaaaacat gtcaattatt actgatgttt acgctcgcga gatcttagac tcacgtggta
     2221 acccaacaat cgaagtagaa gtatacacag aaagcggagc ttttggccgc ggaatggttc
     2281 cttcaggtgc ttcaactggt gaatacgaag cagttgaatt acgtgacgga gacaaagctc
     2341 gttatggcgg taaaggtgta actaaagctg tcgacaacgt aaacaacatc attgctgaag
     2401 caatcattgg ctacgatgtt cgtgaccaaa tggctatcga caaagctatg atcgctttag
     2461 acggaactcc taacaaaggt aaattaggtg cgaacgctat tttaggtgtt tctatcgctg
     2521 ttgcacgtgc agctgctgac tacctagaag tacctttata ccactaccta ggcggattca
     2581 acacaaaagt attgccaaca ccaatgatga acatcatcaa cggcggatca catgctgata
     2641 actcaatcga cttccaagaa ttcatgatca tgcctgttgg cgctccaaca ttcaaagaag
     2701 ctctacgtat gggtgctgaa gtattccacg cattagcttc aatcttgaaa gctcgcggat
     2761 tagcaacttc tgttggtgac gaaggtggat ttgctccaaa ccttggttca aacgaagaag
     2821 gttttgaagt aatcatcgaa gcaatcgaaa aagctggcta tgtacctggt aaagacgttg
     2881 ttcttgctat ggatgctgct tcttcagaat tctacgacaa agaaaaaggt gtttacgtac
     2941 tagctgattc aggcgaaggc gaaaaaacaa ctgacgaaat gatcaaattc tacgaagaat
     3001 tagtttctaa atatccaatc atctcaatcg aagacggatt agacgaaaac gactgggacg
     3061 gattcaagaa attaactgac gtattaggcg acaaagttca attagttggt gacgacttgt
     3121 tcgtaacaaa cactcaaaaa ttatctgaag gtatcgaaaa aggaattgct aactcaatcc
     3181 ttatcaaagt gaaccaaatc ggtacattaa cagaaacttt cgaagctatc gaaatggcta
     3241 aagaagctgg ctacacagca gttgtatctc accgttctgg tgaaacagaa gattcaacaa
     3301 tctctgatat cgctgttgca acaaacgctg gccaaatcaa aactggttct ctatcacgta
     3361 ctgaccgtat tgctaaatac aaccaattac tacgtatcga agaccaactt ggtgaagttg
     3421 cagaatacaa aggtttgaaa tctttctaca acttaaaaaa caaataattc ttttctaatg
     3481 aagatacatg atcgactatt gaaagatagt cgagtatgat cacttagtta agtaatcgaa
     3541 ctctctactt gctgaaatat cagcaggtag agagtttt
//
LOCUS       DAHUVV010000159         3566 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.163, whole genome shotgun sequence.
ACCESSION   DAHUVV010000159 DAHUVV010000000
VERSION     DAHUVV010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.163"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..248)
                     /locus_tag="NK767_002774"
     CDS             complement(<1..248)
                     /locus_tag="NK767_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6695706.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIY"
     gene            complement(364..930)
                     /locus_tag="NK767_002775"
     CDS             complement(364..930)
                     /locus_tag="NK767_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HCH6695707.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(1066..1719)
                     /locus_tag="NK767_002776"
     CDS             complement(1066..1719)
                     /locus_tag="NK767_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695708.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(1769..2197)
                     /locus_tag="NK767_002777"
     CDS             complement(1769..2197)
                     /locus_tag="NK767_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCH6695709.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(2202..2576)
                     /locus_tag="NK767_002778"
     CDS             complement(2202..2576)
                     /locus_tag="NK767_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6695710.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            2880..3065
                     /locus_tag="NK767_002779"
     CDS             2880..3065
                     /locus_tag="NK767_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6695711.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            3078..3326
                     /locus_tag="NK767_002780"
     CDS             3078..3326
                     /locus_tag="NK767_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695712.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            3357..3527
                     /locus_tag="NK767_002781"
     CDS             3357..3527
                     /locus_tag="NK767_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695713.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
BASE COUNT         1032 a          640 c          623 g         1271 t
ORIGIN      
        1 ttgtatataa ctaaccattc ttcaacaagc tcatgaaatg ttagatcagg cttatgaagt
       61 gttttttcgt ttaattttat atcaattttt ttatttaact ctagcaatgc cactttctga
      121 gtttctcgag atttattttt ataagtaatg ctcacttttt ttctttttct agtttttgga
      181 tcaatatatc gctcattata tttatatact ttttttccgt ttttatcagt tttagtttcg
      241 atccacattg ttttcatctc ctatttgttg ctataatagg aatagataag taagccgatt
      301 atagcaggtt tatttttcat cacgtccaca aactttggcg agggaggggc gtgtttttgt
      361 tagttattgt ggtaggttat ctaaagcgta ttgtgcttgc tcatttgtaa aaccttcaaa
      421 tataagttga tcgtacaatt gcgaatctga cattgatgcc caatcatcgt aactttccgc
      481 tttagccaaa gcttgttcgt tgtaatcgac atcaacatta gccagcgcat aatcgattgc
      541 atcacttgga tattcttcaa actctagttg ttcacgcaaa ccttgttcag acataccagc
      601 ccaacctaga tagctttcag ctttgctcaa tgcatttctg tattcacgag gaacactttc
      661 ttcttgagta acttcttcta tagtagtttc agtagtttca gtttcttctt caacgactgt
      721 agattctgct accgattctg cagaagacac ttctgtcacg ctttctttag ttgtttcagc
      781 gacggtattt ttagcatcat cactgcctcc ttgcgaacct atagcaataa acaccacaac
      841 aaccgctaac aaccaaaacc atacccgttt ataaaacggt ttcttaactt tatacatttt
      901 cccatcttga cccataactt tttttgccat ttaaatatcc ctcgcttctt gttataatat
      961 atttgtgatc tcagaaatga ggtatgagtc cgtgttgcag cacgggcttt tttctttata
     1021 acttttttag agattatagg caaaatagta gggcataaaa atgtattatt gaattccgta
     1081 tttagaaaat cctaattgaa cttcaccgga agtcttttgc tgtgttgtac gcaatgcttc
     1141 ttcttcagac attccattct gtactttcca tgcaacaggc gacatcccgt atttgttaac
     1201 aaaatcagta agtgataaag tgtcagcgtc ttgctgagcg cttgtttgtt ggtcttctgg
     1261 attctgttga gatgctgctt gttgttcttt ctgatcttga ctgataatat tgccagcatc
     1321 atctgtagtc aatccatttt cataaagggc cacgccgaaa gcttcccact ctttgttgga
     1381 ccaatttgca cgatcagctg gagttgactg taaagtgcgt tgtttcatct gttcatatgt
     1441 ttcttcttga ggtgcggttt ggattgtact ctgactggag cttataactg ttgggctagg
     1501 ttccgccgta gcttggttgg agcttgtaac tgttgaacta gaatctgttt tagatgtaga
     1561 tttgctagta gatgaactgg tttcagttgt ttcttttgtt ttacttattt ttgtttcttg
     1621 gttagaagtg gtatctgttg attcagcttt tttattattt gaacaagctg aaagtagcag
     1681 agcagtactt aacaacaaca taacgctaac ttttttcatt ttataattcc tctttctcgt
     1741 tgtaatatgt gtgctaacac gggctttttt atataagaaa acgataagcg ctttctggaa
     1801 gcccgtaaag attctttaat tcctcgattt ttttaggata ttgatcatta tcttctttat
     1861 aaagagaaac aatgagatta gcagcaaagc aattagcttc gctttcagat ttgcttctag
     1921 atgttcttgt tgatacataa tagctggata agccacgatg aaaaatagcg tgacctaatt
     1981 cgtgagcgca aatgtagaat ctttcctcag agtctcgcag ttcatcattt aaaaagatta
     2041 tcgcacgatc tctaatttct tgaaactgcc ccttgggatt ctcgataaaa ggaacgtatt
     2101 gaattttaat gcccatcttt tcacaaatat aaaaaggatt agcggactgg tatttccgct
     2161 tcaactcctc gactaaatta atcgtatcca tctccataag ctcacatctt tttgcctttt
     2221 tctttgtctt ctttcacaat atcccagaaa gtcgctatca ggatatcttt tacgcgctgt
     2281 atttgttcgg gtgtcaatgt ttccccacca taagacatat taacatttga gtctagtagt
     2341 ttatcaagtt caaccacttc ctcttttgta gcccacttgg gaacattatt atttcccaat
     2401 aaataatcag ttgtgacacc aaaataatca gcaactttct ttaagttctc agattttggc
     2461 gaggctttat cccatcttct tatttgtcca ttagagatgc ccacctgtct ttctacttct
     2521 gctatagtca catgcttttc gtctgctaat tctttaatct tagtaactaa actcattatt
     2581 atcaaccttt caaagctgaa aagaaaataa atagcttaaa agttatattt ttggttgaca
     2641 attagctttt aagctagtat atttaattcg taagctaaat tgttagctaa ataagagcaa
     2701 caaaaaactc tactaattta aaacattctc tcggtcgcca aacttagaaa tgttatttta
     2761 gaggcttttt ataagtctta tttaactatg tattcataat agcttaaaag ctaataggtg
     2821 tcaacgattt agctaatttt ttagcttaca aattatttgt ttagaaagga gctattttta
     2881 tgtctgagaa tttagactta aaaattcgag cggagatgag aaaaagaaga atgactttca
     2941 aagaactagc tgcgcttatc ggtatttcag gagcttattt atcagatatt ctaaacggca
     3001 atcgtgatgg aaagaaagca caacagcata tcgaaacagt gaaaaaaata ttggacatcc
     3061 gatagggggg gtaggcgatg acaaaactaa aaaaacaaga ttttgtaaaa aaatataatt
     3121 attctccatc tacttatcaa cgtcgaatgt cggaactaaa aaatacagca attttctcag
     3181 cggcgtatga acgggtcaca ggacaagaag tttggatcaa tacagaatta tacgataaat
     3241 ttttgtcttt caaatcctat aacaggttac gcacaagaaa ggtaacacct aaagaattta
     3301 tcgagaagca tttagttgat ttataaaaaa cagaacattt tgagaggtga aggttaatgg
     3361 gtaaattcaa cagagcatta gtattcagcg caccgctaat cgtctatgct ttagggcttt
     3421 ggggaagtag acaagcgttg ataggaacga ttgtgtacat ggtttggatt tttatagggc
     3481 tggatgaagc tgagtacaaa acaaaaaagc cagtcgggag ggactgacta atgagtaagg
     3541 acgatattgt atttctgtta attatg
//
LOCUS       DAHUVV010000160         3561 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.164, whole genome shotgun sequence.
ACCESSION   DAHUVV010000160 DAHUVV010000000
VERSION     DAHUVV010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.164"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            238..558
                     /locus_tag="NK767_002782"
     CDS             238..558
                     /locus_tag="NK767_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCH6695714.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            719..1624
                     /locus_tag="NK767_002783"
     CDS             719..1624
                     /locus_tag="NK767_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6695715.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1621..2394
                     /locus_tag="NK767_002784"
     CDS             1621..2394
                     /locus_tag="NK767_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCH6695716.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(2571..3482)
                     /locus_tag="NK767_002785"
                     /pseudo
     CDS             complement(2571..3482)
                     /locus_tag="NK767_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT         1119 a          693 c          671 g         1078 t
ORIGIN      
        1 tctatgaaat tgcaaagcat tcatcttctt ttctgctaac gatatatccg tcaatgcttg
       61 ttacacttta ctaaaaagta tgagattgcc gatatctgga taggtgtgct atactacaga
      121 aaaagatata cgatctgcca ccgggtagta aaaaagagga tgatcattcc attaggtctt
      181 tgaagggatg attctcctct ttttgtgtat tttttatatt gaaggaggaa cgtatgtatg
      241 gcatggatgg aattaatcat agcaggaatt ttggaagtat tctggtcaac gatgatgaaa
      301 tggagtgatg gtttttctaa aatcaattat tctttttaca cagtgattgg aatgattgct
      361 agtttttact ttttgtcaaa ggcaatcaaa agtttgccta tgagtctctc ttatcctatt
      421 tggacaggga ttggcgcagt tggttcggtc attattggtg tggtcttgtt tcatgataag
      481 ctgaatcttt ccacttggtt ctttgtcggg ttgctgctca tcagcatcat tggcatcaaa
      541 atcactagcg gtcactagat acttagacaa gcagacaagg actgatcaga aaaagcgggg
      601 ctttttattt gatattcctc tttctatgaa aaaacaagaa aataagacta atgacctagc
      661 gacttctcta ctcatcaggt gtatgcttgt actatctata gaaaaggagt gaatattttt
      721 gaaaagtctt tcggtccaag aattatccaa gaactttggt cacaaacaag ccctcagcga
      781 tgtcagtttt agcttatctc ctggcaaaat cgttggatta gtaggtccta atggcgctgg
      841 aaaaacaacg atcatgaaag ctattttagg actgatccat tactcagaag gaacgatccg
      901 tattggtgaa aatgagattt ctccctctag tcataaagga ttagaacaag taggagcatt
      961 gatcgaatat ccgggtatct atccattctt aacaggttat gatcatctga agttattctc
     1021 tgaaacaaac gatgtatctg ctatcgacac tatcgtgaaa cagctcaaga tggaaaaata
     1081 cattcacaaa aaagcgaaat cctactcgct gggaatgaaa caaaaattgg gaattgctct
     1141 tgccttactg aaccatccgt ctatcgttat tttagatgag ccgatgaacg gtttggatcc
     1201 acaagcaaca agagatgtgc gaaaaatcat taccgacttg gctactcagg gcacctcttt
     1261 cttgatctcc agtcacttac tcagcgagct tgaaaaaatg gtcgatgagc tgatcctgat
     1321 caatcaagga aaaatcatca aacaatgtac aatggctgaa ttgaaccaaa cagcgcaaga
     1381 tttcatcgtt atctctacga caaatgatca agctgctcga acgatcttga cagacgctgg
     1441 atatcccttg gaagatcctg ataaaatcaa gcttctaaaa aaaaacgaag agttattagg
     1501 aactgttatc cagttattgt ctgcagaaaa tgtccatgtg acagatgtcc agcatctgac
     1561 gaatgatttg gaatcttccc tattggaaat tctggattca gtaaaggggg aagaagaaaa
     1621 atgacgttgc ttataaaaca agaattattt aaattgatca agaaaaagtc aactgctgtt
     1681 ttatctgttc tattagttgc tttgctcata ggaacagcac ttctagccaa aaaatacacg
     1741 acgatcattg atccagttga aatgactgct cagcttttca gtgccacatc ttggattgtc
     1801 tttatcatga ttgctgctgc aagtacgatc atttcaatgg aatcacaata tggtacattg
     1861 aaaaatctcc tttatcgaaa gtattctcga ggagaaatat tagtaagtaa atggatcaca
     1921 ttagttattt attctattta tttatacctt ttagctatta ttgtcaccgt gctgatgaaa
     1981 ctgatccttt tcccttcgat ttcctttacg gagaagattt ctactggtca gacactgatc
     2041 caaagcttat tcacttatac tttgggtagt tatatcggtt tatggctaat tttaagttta
     2101 gtgttgatga ttgcttgttt tatcaatagt tccggtgcct ccatctcagc agggatcgtc
     2161 ttttattttg cttcatcaat catatctggt atcctcatcg cactcattca aaaatgggag
     2221 tggatcaaat ggaatccaat cagtatgctg aatctgcaaa accaaattgg caacgaagag
     2281 attatgaagc aattgactca tctttctact aatcaaatgc tgtttggcaa tttagcttac
     2341 attgctttgt tcttagcatt gggatacctc gtattcaagc gaaaaaatgt atgatggttc
     2401 attaacaaag aagttgtgac agaagtggcc agtttcaaga aatttcggct tatttttaga
     2461 agaagctact tcttgaacac catttattca aaaaaagaag cgatgacacg aatgttgtca
     2521 ccgcttcttt tttgaataaa ccacaggctt cacagcctct ctttatactt ctatacgtga
     2581 agcatcccag tggcaaataa aaccattgcg attactccga ccatcgtcac accgataagt
     2641 aaaatcttat ctaagtggat atgttgtaca ggttcgtttt gttctttacg tgctttgagg
     2701 taaaaataaa tacctggtaa gaaaagaaca agaacaagca tcacttcatt aaaggctaca
     2761 actagcatgg cccatacttg aaaagcactt gctaaaaacc ctactaaaaa ttggacccac
     2821 tctttccgct gataagaaag cttcatttgg tacagcccta agaaaagcca cgtgaagaaa
     2881 atcgctgctg taccaagtgt ataagcaaag ttatatgcac tgtcagtgaa taataatgaa
     2941 agtaaaaaga cattcgtaca taacgtcgtc aatacgatag cataagttgg tgccccattt
     3001 ttgttgagtt ttccccatgc tttaggcaaa agtccgtcct cagccatatt ccccattgtt
     3061 tcaatcggca agattgtcca tgagagccaa accccaacta aagaaatgat caaaccgccg
     3121 ttgatcacga ccgcccccca tttaccaacc atcatttcaa atacatatcc catagcaggt
     3181 tgcgctttga tcgtttgcag ctgattcatc gtcattgtgc catatggaag catggaaacc
     3241 aagacgtaaa taatgatcaa accgaatatt cctagaatcg ttgctttccc aacatccgat
     3301 tgtttttttg ctcgtgaacc tacgacagaa gctccttcaa taccaacgaa tacccagagc
     3361 atcaccatga tgcaactcaa tacttgacta gaaaacggga tcggctgccc attcgcattc
     3421 aatatgatat cgctagaaaa gttattaatg aattgttgga tcgaaaaggt gctgatagct
     3481 acgtaagcgc cccatagaac agtaccttac taacaaatgc acctcaagat atacttgagc
     3541 tatcaagaag tctggaggtt t
//
LOCUS       DAHUVV010000161         3476 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.165, whole genome shotgun sequence.
ACCESSION   DAHUVV010000161 DAHUVV010000000
VERSION     DAHUVV010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.165"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..2232)
                     /locus_tag="NK767_002786"
     CDS             complement(<1..2232)
                     /locus_tag="NK767_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695717.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="NK767_002787"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="NK767_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          976 a          769 c          549 g         1182 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataa
//
LOCUS       DAHUVV010000162         3449 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.166, whole genome shotgun sequence.
ACCESSION   DAHUVV010000162 DAHUVV010000000
VERSION     DAHUVV010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.166"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(150..1337)
                     /locus_tag="NK767_002788"
     CDS             complement(150..1337)
                     /locus_tag="NK767_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HCH6695718.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            1447..1824
                     /locus_tag="NK767_002789"
                     /pseudo
     CDS             1447..1824
                     /locus_tag="NK767_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            1872..3272
                     /gene="yfcC"
                     /locus_tag="NK767_002790"
     CDS             1872..3272
                     /gene="yfcC"
                     /locus_tag="NK767_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCH6695719.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            3305..>3449
                     /locus_tag="NK767_002791"
     CDS             3305..>3449
                     /locus_tag="NK767_002791"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695720.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          978 a          652 c          684 g         1135 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact tttacacaat tatatggacg
     1441 taatcaaaaa atgtttccac agaggacttc tttaattatt gtcgattaga ggaaagtggt
     1501 gtaacagtca ctagcggtac agatttaccg ctgtttatta ctgatattcc tgaatcgatt
     1561 ataagagcag tattccgtcg ttttcctgaa ggagaagaag tctgggagaa agaaacaagc
     1621 ttcacgacaa ccgagctgct gaaaagtttt acaagcaaca gcttcaaagt aaatggttta
     1681 tcggagtacg gagaaataaa agtagggaaa agcgccagct tagctgtttt cagcgaggac
     1741 ctctgtactg atgactggca aaaagtgatg gctgcggaag ttatcgctac ctatcttgat
     1801 ggtgacttag tttacaagaa ataatgcttt tcgatataac gttataaaac gaattaaaga
     1861 gaggttaaaa aatggaaaca atgaaaagaa aattcacctt cccacatagt tatacattga
     1921 tttttatctt aattattttg gctgcaattt taacttggat aattcctagt ggtcagtttg
     1981 agacagttgt tgaaaacgtc gcaggtatgg aaaaaacaac ggttgttccg ggaacctatc
     2041 accaaatcga taaggcagat tatggacaag gtttttcctc tatactaatg gcaccagctg
     2101 aagggattat ttcagctgtt gaggttgttg cttttgttct catcgttggt ggtgccttcg
     2161 ggattatcct aaaaacggga gccatcgatc gagggctgtt taccttagca aaagttctgg
     2221 gagacaaggg gatcttagtt attccgatgt cgatgattct attcagctta ggaggatcaa
     2281 cctttggcat gagcgaggaa gtcattccct tatttgcggt gtttatttct ctgatgtttt
     2341 ctcttggcta tgattccatg actgcgatct tgattctatt tttaagtaca caagcgggat
     2401 atgttgggtc gacgattaat cccttcaacg ttttaatcgc acagggagta gctggcgttt
     2461 acggaaatcc cctgctttgg tttcggatgc tatcctggat tgtcattact gggatttcaa
     2521 ttgttttcac catgctttat gctagcagag tcaaaaagaa tccactgtcc tctattgttt
     2581 atgagagtga tttaaaggtt aagcataagt ttcaaaattt tgacactgaa aaagagtttt
     2641 caacacaaga taaactaatt atcgccggtt ttatcgtagg tttaggaatc atgatttggg
     2701 gtatcatttc caagggatgg tatatggttg aaatttctgc actattctta ggattaggtc
     2761 tttttgctgg gggcatcgct agattttctc aaaaggaaat tgctgaagct tttgtcgaag
     2821 gctgttcaga tttcgcctat gctgctgtaa tcattgggct agctagagga gtcttggtaa
     2881 tcttggaagg tggaatgatt attgacacta ttctaaacag cttggctaac cttttaggtg
     2941 gtcttccaaa attcctgttc actacgattc tactcatttc tcaagtaatt gtttcctttt
     3001 ttgttccatc ctcctcagga gctgcggcat taacgatgcc ggttatggca ccattgggtg
     3061 atctaatgaa tgtaagtcgt gatgttgttg ttctaagtaa ccagtttgga aatggtttaa
     3121 tgaatattat ctccccaact agtggtgtat tactggcagg attagccatc gcagagatta
     3181 atttctctaa atggtttaag gttggcttca aaatatttgc catcttaacc cttgtttctg
     3241 ctgttttgtt atacattgct acactctttt agtcttaatc atccattaat aggaggcaat
     3301 agttatgatt aaaataaatc aattagcagg aatgaatatc cactatttat tttattctct
     3361 caaagaattt ttcgcttatc aacagaaaat tggtatttct tctgttgaac tttggggagg
     3421 tgctccgcat tttttcatag atgctttta
//
LOCUS       DAHUVV010000163         3411 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.167, whole genome shotgun sequence.
ACCESSION   DAHUVV010000163 DAHUVV010000000
VERSION     DAHUVV010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.167"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            160..1200
                     /locus_tag="NK767_002792"
     CDS             160..1200
                     /locus_tag="NK767_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCH6695721.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2024..2356
                     /locus_tag="NK767_002793"
     CDS             2024..2356
                     /locus_tag="NK767_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCH6695722.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2975..3316
                     /locus_tag="NK767_002794"
     CDS             2975..3316
                     /locus_tag="NK767_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCH6695723.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1267 a          461 c          646 g         1037 t
ORIGIN      
        1 ctttaaaggt agtacataat tcattttatt tctgatttta ggtagtattt aattaaaaaa
       61 cagttttaat gcgtttgcat tttttattta tctttgctat tttcgaaaag agtaggtaat
      121 tttacataaa taaaacaacg tgaaagtaga ggatctacaa tgactgattt taaatttttt
      181 aaggcggata gagtttacaa cgaattattt tatcaatttc caaaagtctt tattgtttct
      241 gacgaataca aaaaaatgaa agattcaact aagattgcct atatgctttt aaaagcaaga
      301 ttagagatcg caatcagcaa acggcaaatc gatgaagaag gtaatgttta ttttacttat
      361 acgacaaatg aactatgtag agtattaaac tgccaaaaac aaaaagcgat agcaatcaaa
      421 aaagagttgg aatcctttgg tttattatta caaaagcaga tgggatttaa caaacagtta
      481 gggaaaaata atcctaatag actatatcta gcagaattaa aagtctcaga aaatgatatc
      541 tacttactcg aaaaatttga tagagagaat agggaaaacg ttgataaatc agagggtatg
      601 aaaatcatac ccaccctcga cgaaaaatca gacgctgaat cccttggggc tcaagagggt
      661 atgaaaatca taccgtgcca aaacgttgat aaatcagagg gtatgaaaat cataccagaa
      721 cttaataata atatattaga cactaataga cacaatatag acactgaaaa agaccgccta
      781 caagatcaat tgttgttaga caattttgag acaattatga caaacgacag cattgctacg
      841 tttgtccctg aacgagtatt aaatttgata aaaacatttt cttcaagtta cagtgaagct
      901 caaaaaaccg tccagactat tcataatgca aagaaaaaag ctgaaataga aagtgatatt
      961 tcgatagttt ttgaagaact cgatagttat tatgtcaatg cagaacaaga attatacacg
     1021 acactgttaa aagcctatca aaaattaaaa accgaaaaag tcgaaaatat ccagaacctg
     1081 atttttgtct atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa agtatcaagt
     1141 gaaaaacgtt tgaattatgc aagctcccca agcactatta cgaaagactg gttagagtga
     1201 aactttactg acaaagtgat tgttgcttca tggagtaaca ttaaaattaa aaatttttta
     1261 ttattctaat tatattacga atattacaaa ataatagcta ttttaataga atttataagt
     1321 taaaatatta tgataaaaat cacaaacgta gccttttacg tcataagtgt taaaagaaat
     1381 aattaatttc aaatatagaa aagggaacgt atgtttgcat actccttttt tttgagttat
     1441 aataatacgc gaagagagat aatttcatta tttaaacaag ctaaaaatct tgatattact
     1501 tcttttcagg caatacgaat attattcgct tacatttttt gtataaattt aattgtctgc
     1561 tatgcttgtg atgaattgat tttcgttttg cacttttgga tgataaattt ggacaattga
     1621 aaatacaaaa aaaagaaagg cggtatgaac tcggaaggct ggtaaccttc gtcagcgttc
     1681 atacacggca ttaagatttg gcccttaatg cgttaacctt tcagtgttac ggtattaccc
     1741 gcaatggatt tagtataaca gatattcaaa agtgatacca ctttttttgg ctgtgttaag
     1801 gttaaaaata cgtgatatta cgatgaatct atgcctaagc ataatgttat aaaaatgtcc
     1861 gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt ttggatgcgt
     1921 tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa aataacaaga
     1981 aaaacttgtt aaaaatattt aaagaaattg ggtggtttat tttatgtcat atacaggaat
     2041 tttgagtctc aaagatattt gtcactacgg taaacggtgt accgctacag agaaaatcac
     2101 gaagaaacta tcaactggcc aaagtaaaac cgttgtgcag tgtaagaaat atataatcca
     2161 gaaagacaaa gtctcagagg aaatgattta ttacattggg aaacaaaagc aaataatttt
     2221 aaaagatcct atccccttaa aagagctata tccaacaatc aaacatgttt atgaccaaaa
     2281 cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa aagggatggg
     2341 gcgtttgatc ggctgaattg atcattctca taacttgtat tttatcgttt ttcgcttgct
     2401 tgaaaatatt gtcgagactt ctaaaggaac ttagagtcac tagaaaaata aggagtagac
     2461 ttgatctatc aacaattccc ttcggggagt gacggggtag gttttaacga ttggtaacgt
     2521 aaaaaattgg tcaatcgagt aaatgcgctt gtttggatag tggtgaaatg aatttgtgta
     2581 aataataagc gcagagtaaa caagagggaa tttagaagtt agacaagtta gaatagcgtc
     2641 tagattaatc atgagcagct actaagggat ctagactttg acgcaaactc atggaataag
     2701 gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg ctgcagtaca
     2761 tgtagatact tgcgaggacg ttgattattg gatcaatcga gcatttaccg ctgtctacta
     2821 gggtttctcg tttcggacgc cataaacgag tcgaaatttt ttatgtattt ttaagtaaac
     2881 gaaaaacgat aaaatatgaa caagtcctga ataaagtgaa aaaattaata taaagcatag
     2941 agaaaatgaa atttacgtaa tggaggaatg acaaatgaaa aattatgatc cgaatatacg
     3001 attgggcaca catactatta aggtttcttt ccaacgttgg gattacaaag gatttgtgac
     3061 gtttagaagg ggcggaaatt gtaaaggatt agatgtttta gcattagacg aggatgattt
     3121 atacgatcaa acattgactg ataatccaat tggattcggc ttgctacctg aagatgatga
     3181 aggtaatgaa tggtttaaaa tgacgcttat gaatgataat ggagacgaat tatctgttga
     3241 agatacttgg tcatatttga gtgattatat tgtcagcttt gaaatcattg aatttgtagc
     3301 ggataaagaa gaataaaaac tggaggaata aattttacag ataaaggctt agaaggagtc
     3361 acgacgatta ataaaaaggt agtattacct taggcattga gtaaggaatt t
//
LOCUS       DAHUVV010000164         3289 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.168, whole genome shotgun sequence.
ACCESSION   DAHUVV010000164 DAHUVV010000000
VERSION     DAHUVV010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.168"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(451..1761)
                     /locus_tag="NK767_002795"
     CDS             complement(451..1761)
                     /locus_tag="NK767_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCH6695724.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="NK767_002796"
     CDS             complement(1773..3191)
                     /locus_tag="NK767_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6695725.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         1005 a          671 c          510 g         1103 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatct
//
LOCUS       DAHUVV010000165         3200 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.169, whole genome shotgun sequence.
ACCESSION   DAHUVV010000165 DAHUVV010000000
VERSION     DAHUVV010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.169"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(552..2165)
                     /locus_tag="NK767_002797"
     CDS             complement(552..2165)
                     /locus_tag="NK767_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCH6695726.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..>3200)
                     /locus_tag="NK767_002798"
     CDS             complement(2306..>3200)
                     /locus_tag="NK767_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6695727.1"
                     /translation="TNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANLYAEFA
                     HKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFSIDQEI
                     TSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCLQKIQL
                     SSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLLEQLAQ
                     IFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGFHSIHH
                     FSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1029 a          548 c          570 g         1053 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt
//
LOCUS       DAHUVV010000166         3082 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.170, whole genome shotgun sequence.
ACCESSION   DAHUVV010000166 DAHUVV010000000
VERSION     DAHUVV010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.170"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(299..913)
                     /locus_tag="NK767_002799"
     CDS             complement(299..913)
                     /locus_tag="NK767_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCH6695728.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1519..1896)
                     /locus_tag="NK767_002800"
     CDS             complement(1519..1896)
                     /locus_tag="NK767_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695729.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2196..3077)
                     /gene="galU"
                     /locus_tag="NK767_002801"
     CDS             complement(2196..3077)
                     /gene="galU"
                     /locus_tag="NK767_002801"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCH6695730.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          933 a          644 c          476 g         1029 t
ORIGIN      
        1 gtattaagag acaagaaaga atccatatgg attctttctt gtctcttttt ccactacttt
       61 ttatttttct gacactacat attcttataa ctcatttctc ataactgtga aacaacttat
      121 aaaaataaca aactttaaac aaaacaagct taaaatcaaa gatgatccat cttaaaataa
      181 ctaaaacaaa aaaagtagtc gttgttaatc ctcattaata atcgttttct ttttttcaaa
      241 cgctccattt ttatgtggaa cattattttt ttaaggaact ttgtcaacat gtggataact
      301 attttccact tgtgtcctgg actagctgtt ttgctgtaaa atacaaaact tgctgcagca
      361 aaaagatcgt tgcaagagag gctgttccgc agatcgctgg atctagattt ggcaaagtca
      421 agactgcatc atgtaaattg gcgtggttgt ccaaaaccaa atcagcaaat tcaaataatc
      481 gcaaaccact gctatgttgc gatttgatac ttcgtgacaa atcaaatcca gtaacaatga
      541 ttaatggatg accattttct ttgatccact gagctagttc gatgattgcc gggtttcttc
      601 cttcattcga aagaagaaga aaaatgtctt ctgatttgac agaaagctga tcaataattg
      661 cctgtcctgc accttctagc gtttcataga cacctccggc tggattttca atcaatatac
      721 ttggtacaag atccggggtt tgatcccaaa attcaaatgc tacaccactt agtgtgcggc
      781 tagcatatat ctgcaatcta ccaccttgat taatttgttt cgcgatccaa gtgccagctt
      841 gaagcatcag cggctcttct atccttaatt tctcttccaa ttggtgataa tagtcaaaat
      901 aacataaact catttatctc tcccgcctag ctatacttga catgaataga aacagctgcc
      961 taatctaagg aagtacataa aaaaacagta gttagacagt aacacatgtg gtctttgctg
     1021 tttcaaacac aaaaaaggca ttgaacgaat acgcccaata ccttgacata gcaaataaaa
     1081 caacgtgcag accatggaaa atatctacat aggggtagaa ttttcaccct agattaggta
     1141 caataaatta ggtcgatgca ctgcattgac tatggcaaaa tgatcggtca gggtgtatgt
     1201 gtgtgactgg tcattccctg attcttagcg ccaactaagg gtcagggagc ctttttttat
     1261 tcagctgttt ctatctgaat ttattgtaag taatctctta tccaagtgca agcaagaaaa
     1321 tacaaaaaaa cattggtatg atttatatat atataaataa cataaaaaaa ggaatggctt
     1381 atcccgatac aacaagcatt cctttaaaaa agattctttt atataagtga aaaaagcttt
     1441 tttccaccaa ttaaataaat ctctcatctc aatagtttat ccattatttt atcttttttt
     1501 gcgtatacct actaaatatt accgtcttct tctcgttctt ctactttgtt cgagagaatc
     1561 tgtactagaa aaagtagttg tatgtctacc ttttctgcct atccgagact ttcttttcat
     1621 atatgggata taaatgatta gatacagtac agcaaaaaat aaagcaaata cgcaagcttc
     1681 ccaactacct aacgccaatt caaccatcaa ggtagcaacc ccaacggatg ctgcatacca
     1741 gataaaaatc agaaacgttt ctttttttct gaccatgcga aacatgataa atagactaat
     1801 aagcaatagt ggcaacacaa atatccaatt gcttccttcc atcagccact tgagtatatc
     1861 aagcagcgac tgtgcaacag cgactaattt tcccatagtg aacacacacc tttttttata
     1921 attaaaaaat tcgccttttt attctactat gaatcttctg gcttggcttt catttctctt
     1981 atgtaatcat tttcttttat acaacttaaa aaaaaattaa accattagca taatattttc
     2041 tgaatctatt cacgaaagtc tttttctatc tttcgttttc cacgactcaa ctgatctaga
     2101 gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa gctgccgctt taataacaaa
     2161 tagcctttca ttctcagcag gccacttatt tccagttaac tttctgttct tagcttttta
     2221 ctcaactgga taatatatgc acttaatgaa gtcccaattt ccgaatgttg aagtccatat
     2281 tcaatatttg tcttcatata tcctaactta tcgcctacgt cgtatctttt tcctttaaac
     2341 tctctcgcat agaccgcctc tgtctggtta agagtctgaa gtgcatccgt tagctgtatc
     2401 tcgtttcctg ctcctggttc ttgagtttct aatatctcaa atattttagg agtcagtaga
     2461 taacgtccga taatggctaa attacttggt gcttttccca tttcaggttt ttctacaaac
     2521 ccttttactc tgcaacgacc cttttcgcta tctgttttag gatcaataat tccgtattta
     2581 gatgtttcat tttctggtac acgcatgacg gcaacagcgg aagcttgtgt ttcttcataa
     2641 tccatcatca attgttgagt caatgggata tcatctttca tcaaatcatc ccccagcatg
     2701 acgacaaacg gttcatttcc tataaatgct tttgcttgaa gcacggcatc ccccagtcct
     2761 cttggatgag attgacggat gaagtgaaga ttgacttgag tcgtttcttc aactaaagcg
     2821 agtaattctg ttttttcctt ttcccgcaaa ttgttttcca attcgatatt tgcatcaaag
     2881 tgatcttcaa tgggtctttt ttcttttccc gttacgatca agatgtcttc gattcccgaa
     2941 tccaatgctt cttccacaat aaactggatc gttggtttat ccacgattgg aagcatttct
     3001 tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta gcccagcggc aggaatgact
     3061 gcttttttga ccttcacttg ct
//
LOCUS       DAHUVV010000167         3073 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.171, whole genome shotgun sequence.
ACCESSION   DAHUVV010000167 DAHUVV010000000
VERSION     DAHUVV010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.171"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..221)
                     /locus_tag="NK767_002802"
     CDS             complement(<1..221)
                     /locus_tag="NK767_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695731.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITG"
     gene            complement(218..379)
                     /locus_tag="NK767_002803"
     CDS             complement(218..379)
                     /locus_tag="NK767_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCH6695732.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(394..753)
                     /locus_tag="NK767_002804"
     CDS             complement(394..753)
                     /locus_tag="NK767_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HCH6695733.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            complement(750..1601)
                     /locus_tag="NK767_002805"
     CDS             complement(750..1601)
                     /locus_tag="NK767_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCH6695734.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(1618..2448)
                     /locus_tag="NK767_002806"
     CDS             complement(1618..2448)
                     /locus_tag="NK767_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HCH6695735.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(2451..3053)
                     /locus_tag="NK767_002807"
     CDS             complement(2451..3053)
                     /locus_tag="NK767_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695736.1"
                     /translation="MNIDRLKTMYDGYDGDRVIEIRFIDPRRFTVQQRNFIYALIGDI
                     FIDTGMPTDFWKEFFYFRFEGVTGRKISLKDESNTTVSDANILANIILDFIFEHHIPF
                     KEGYEILPANQEYYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAA
                     LCRIHHTEKHKIGVINFKNKYQIKGIKLNQETIKKLRIGG"
BASE COUNT          742 a          670 c          486 g         1175 t
ORIGIN      
        1 aatccagtaa tttcagataa ctgttcagct gtacctgtta ctagaattcg atcaccatgc
       61 cagattgcaa tttttctcgg cgttttccgt ttaggctttt cagtccacat tgatttaccg
      121 agcttttgga cttctgcaac tatttctttg tcttcctgcc aagattctga cttggttaat
      181 tcagcaattc gtttcattgt cgctttctta tccacgctca ttcctccaat ctacgaattt
      241 cccttcttaa gttctctatg tgcaaatcga ttgccttcct cgccgtttca ttgaccatca
      301 ctgcctttgt tcgttccaga tcgtcaatct cacgctgaag gcttcgaata cgcatttgaa
      361 tcacttcttc tgttgtcatg atggaccacc tcgctaaaaa cgctcttcct tgaacgtatt
      421 ccgatatttt ttagctaata tcaacggcgc ttgatattga tgacagaaca actttgcctt
      481 gatcttaaag tcttttgtct gcattccttt gacatctacg actttgacaa gtttgccgtt
      541 tttataaaat gtgaagtcgg gaatatactc gatcttgcga tacttctttc cgtctagttc
      601 aaatttcggc atcagctcaa atctttcctg aagttttact ttccagccat ttgcttcagc
      661 ttgccacaag gctagatcgt aatactctgc ttctgcgata gaatcaaact tgatacctcg
      721 atgaacagtt tttttattac ggtatttatt catgcgatac tacctttcac tggttttatg
      781 cgcttgtctg ctgtttgttg gaatttcagc gcataacctt ctgaattctt aaatatccta
      841 gaaacaattc tttcaccgta ggcttctctt agttcaggac cagataagtt tgttgtgatg
      901 atcgttgcct tgttctgtct ggcttctaag agcgtgttta acgtgttgtt tgtaaactgc
      961 ctactatttg ataccccact acctaattca gctccaatat cgtcaaaaac caccaaatca
     1021 gttgttttaa tatcggctat aagcgatcct tcaatttctt ttctcagttc agtattgtta
     1081 taagaaaact ttatttgctc taataattct tgatagctta taaaaagtat tttcttgtca
     1141 taatttgagc gctcaagtat ttcccaagct gtggccattg ataagtggct ttttccgctt
     1201 cctgatttcc ctgatagaat gaaatgtgca ggatggttca gcagaacctc attcgtatag
     1261 ctttttgctt tttctaaagc gatttttgtt tcttgatcca ctacgtgata attttccatt
     1321 ttgcatttaa acaaagtttt atctgttaat accgaaccat tttgaaaaaa actcaacgct
     1381 cgtgctttta agctatcgtt atatatccgt tcggtctgta tatcctcttt cacacgtaac
     1441 gctttataac cacaactcat gcatgttggt ttacaacgtt ctgaaccatc cttattttta
     1501 gctcgccaac tatacaaagg ttcgctacat tctggacatt ttccgctttg cactaatact
     1561 cttcttatta gtttctccat agcatttgct aggctttcca tgtgatgcat ctccttttta
     1621 aattggcaag tcgtcatatt cactaggatt gctgtactgt agtttttgac tttgcttttt
     1681 atgattcttc ttgtctgctt tgatttcgaa tttgagcttc tcaaattttt ctctcaattt
     1741 cttagcactt ctaatatttc caaaccaaaa ttcatttgta ggtagccaat tgatcacata
     1801 ctcaatcgct tctatagatg ctttatctct ttcttccatc aacctgattg tgtctgccca
     1861 tttttcgata tctactttgt tcatttcttt tggaaaatct tcagttaaat tactttgcaa
     1921 ctttttagca aggcgtaagt gttcgtcaga atacttacct ttcttttctt ctttatctat
     1981 atctttatct tcttctatat ctttatctgt accgtcacgt gacgtcacgc taacgtcatt
     2041 ttccaatttg agacgttcct gtctctttct ttccctgtat ttacggtttc tttcagcatt
     2101 ttttagcctt actttatcca taccctcgat attttgatgt ttttcccaat tactgatggc
     2161 aattagtcca tcactgctta gatcaatcat gttgaaattt gccaatgtag ttagcgctaa
     2221 gcgaaccgta tttacgtttt tgccaaacaa tgtagcaagc atttcttcgg tataaggcat
     2281 gttcctctgg atatatatca gaccatcgtc gttagtcttt cctgctaaaa ctagtaatcg
     2341 aatccatata acgatgatgg catccgactc aggaacagct tggattaatc gtattttttc
     2401 atcgtcaaac atagtagttt taagtttgat ccaacttatc tcagccaaat ttatcctcct
     2461 atccttaact ttttaattgt ttcctggttt aacttgatcc ctttgatttg atatttattt
     2521 ttgaaattga tcacacctat tttgtgtttc tctgtgtgat ggattctgca gagtgctgca
     2581 aatgtgtact ctgaatgatc aacttctttg cgctttcgtc ttcccagcgc tttgtcaaag
     2641 tgatcgatat cagctcctgt tttgccacag atgcagcaga ctctttttgt aatgcatttg
     2701 tagaagtaat actcttgatt cgctggtaaa atctcatatc cttctttgaa aggaatatga
     2761 tgttcaaaga tgaaatctag gatgatattc gctaagatat tggcatcact cacggttgta
     2821 ttcgattcgt ctttcaggct tattttgcgc cctgtgacac cttcaaaacg aaagtagaag
     2881 aattccttcc agaagtccgt tggcatgcct gtatcgataa aaatatcgcc tatcagcgca
     2941 tagatgaagt ttcgttgctg tacggtgaaa cgtctaggat caataaaacg aatttcaatg
     3001 actcgatcac catcgtagcc gtcatacatc gtctttagtc gatcaatgtt cacttcctca
     3061 ttgatggttg cgc
//
LOCUS       DAHUVV010000168         2803 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.173, whole genome shotgun sequence.
ACCESSION   DAHUVV010000168 DAHUVV010000000
VERSION     DAHUVV010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.173"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            387..575
                     /locus_tag="NK767_002808"
     CDS             387..575
                     /locus_tag="NK767_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCH6695737.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            653..1666
                     /locus_tag="NK767_002809"
     CDS             653..1666
                     /locus_tag="NK767_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCH6695738.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1733..2767)
                     /locus_tag="NK767_002810"
     CDS             complement(1733..2767)
                     /locus_tag="NK767_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6695739.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
BASE COUNT          869 a          536 c          544 g          854 t
ORIGIN      
        1 ttttagttag tctacattat tttggcgatt tgccaataag gactgttgag ttgtgcaaaa
       61 tcaaatctgg gtcagaatga acgtcattct gtctcagatt tttcgttatt tcaatttgat
      121 taactgattg atcaaaaaga tttttatttt catattctca aatgattcaa cactaaatat
      181 acatgtttga cgcggaaatt attattcgat tttggcggca tcagggggga tgacgacgta
      241 gcagacccat tattttactc aagaaaatgt ccgtgcctat tatttagaat acgatagtga
      301 acgtgctggg agttttgttc ttttagagaa agtctctgaa gataaattag ttgtcttaga
      361 ttttgactgt aacgaaaaat ggcgaaatgg aagagcacca gcaagtgatt gaccggatct
      421 acgaagcgac acgatatctg cctttggatc gtttgtgctt aagtcctcaa tgtggatttg
      481 cttcaacaga agaagaaaat gttttgtttg aagaacaatg gaaaaaattt cttttgtcaa
      541 aagcattgca gaagaagttt ggggaaaatc attaaatcat ctaggagagc ttgataggaa
      601 ataacactat ggtataatca gaatgcttaa tactaactac tgagggagcc ttatgaaaaa
      661 gaaaattctt aaatttatcg gatggttctt cggaatcatt cttgggatcg cgtttgcaat
      721 ctttcttgcc ttccagcttt cccccagacc tggagcattc ttgatcaacc atatgttttc
      781 aaatactgtt caaatcactg ataaaaaaac gttcgatcaa gcagaaaaaa acgtggcgaa
      841 aaaaacagat ttgacgtatg agtccaagta taaagagaat acttatgaca tctactatcc
      901 aaaaaacagc aaggggcctg ttcctgttct attttgggta catggtggag gattcgtcgg
      961 cggagataaa tcgggtgtca aagaatttgc gacaaaatta gtttcagacg ccaacatcgc
     1021 agtggttgcg atgaattatg aattagcgcc tgattcagaa taccctaatc aagttcttca
     1081 agtgaatgaa ctgatcaaag atttattagc tgaagaaaaa gacgacaaat tgctagacat
     1141 ggaacaatta ttttttggtg gagatagtgc aggttcacaa atcgcattgc aatatgcagc
     1201 tatacaaacg aatgatgcgt atgctaaaca aatgaaaatc aaacagctac ttccaaaaga
     1261 tagtctgaaa ggaaccattt cttattgcgg acctgttgat ttgaagctaa cagcacagca
     1321 gcattcggat gatcgattta tgaagttttt cgtcaaaacc gttgcgtggt cgttgattgg
     1381 aacaaaagat tggaaaacga gtgagcaatt gaaagaagct agtgtggctg accatgtttc
     1441 taaggaattt ccgccaacgt atataactga cggaaatgca tactcattcc aggagcaagg
     1501 tcaagcatta gaaaatcggt tgacagaatt aaaggtaccc gtgcaatcct tgttttatac
     1561 agatacaaaa aaagagatta cccatgaata ccaatttgat tacacgctaa aagaatcgca
     1621 aaattgttac cagcagacat tggattttgt gaataagtat aagtaagagg ttgtgaaaaa
     1681 gccgtttcgt tttgagtatg aagaaacgaa gcggcttttc acaacctctc tttcaatacc
     1741 tttttgttct tgggataaaa ctttccctaa caatcaatcg tgcaggataa gtcaaaatga
     1801 ttggttcgtc ccgtataccg tcaattcgct ccaatgccat tcgaacagct gcttgcccaa
     1861 ttgatttcgc attgatttga acactggtca agccagggga gaggtttgcg attgtctcga
     1921 tattatcaaa gcttgctaca acaatctctt ctccaatttg gatatttttt tgtttcaatg
     1981 cccgatagac accaattgcc attggatcgc tcccgacaag aatggcagta ggaagagggg
     2041 attcattttc taatagatta gtcgcaagta cctcaccttg tttttctgtc cagccaccta
     2101 gatgataaga catatatttt tctaagtgat gatcgatcat aaaatgctga taggcacgca
     2161 tcctcttttc atttatagaa atctgcttcc caccgaattc atccatatcg actttatatc
     2221 ccccgatata ggctattctt ttatgaccat tttctaagaa caattccagt acggttttag
     2281 tcatccgttc aagatctgca tacaccatat cgacctcttg ctggatgtca gggttatcca
     2341 cgacgactat ttgcatgttt tgttttaaca aggcggtaat cgattctttc gtaacagtcc
     2401 caacaacgat caccgcacct aactgatcta gatcttccca ttgtttagga ttatcagata
     2461 gattatagat tcgattcatc cctaaatgca gacgtcgagc ttcttcttcc atccctactc
     2521 gcaaatgttt gtaatagata tcctgttctt cgctcgcttt gccgatcgta gagatcacac
     2581 caatcgaagg cattctcttt ttgatatatt tacgagtact gatgtcataa tccaacgcag
     2641 cagctgcctc tactatccgt tgccgagtag tctgagccac atggatcgtc tgatcgttat
     2701 tcaacacacg ggaaactgta gcaggagaga catttgcctt tcgtgcgaca tctctaattc
     2761 caaccatttt tacacactcc tcaaaaaaat aaaaaatttt tat
//
LOCUS       DAHUVV010000169         2771 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.174, whole genome shotgun sequence.
ACCESSION   DAHUVV010000169 DAHUVV010000000
VERSION     DAHUVV010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.174"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(61..303)
                     /locus_tag="NK767_002811"
     CDS             complement(61..303)
                     /locus_tag="NK767_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCH6695740.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(335..892)
                     /locus_tag="NK767_002812"
     CDS             complement(335..892)
                     /locus_tag="NK767_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCH6695741.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(905..1093)
                     /locus_tag="NK767_002813"
     CDS             complement(905..1093)
                     /locus_tag="NK767_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCH6695742.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(1106..1672)
                     /gene="amaP"
                     /locus_tag="NK767_002814"
     CDS             complement(1106..1672)
                     /gene="amaP"
                     /locus_tag="NK767_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCH6695743.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            2089..2262
                     /locus_tag="NK767_002815"
     CDS             2089..2262
                     /locus_tag="NK767_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695744.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          807 a          531 c          404 g         1029 t
ORIGIN      
        1 agtgtcgcta cggttctcaa atttgttctt catttcaaca taagtttttc ttcaatttgc
       61 ttataattta cgtgcaaaga atgaaacgac taatactaaa atgattgcac cgatcaatga
      121 tggaatgata gccattcctg caattgttgg tccccaagtt cctagtaaag cttgtccaat
      181 tgctgaacct actaaaccag caatgatgtt tgcgatgatc cccattgatg atcctttatt
      241 cgtgattgct cctgcaattg ctccaatgat accgccgacg attaatgccc aaataaatcc
      301 cattttaaat tcctcctaac tgacttctta cttcttattg tacacgtggt tcgttatttt
      361 ctttgacttt ttgtgcacct ttgcctacag cagattttgc tttatctgtt tggcgtgaag
      421 caaattgtcc tgtggatttt gctgcttctg ttactttgtc ttgtactgtt tcttgatctt
      481 tttggtattc ttcttgtgtt ttgatgtctg caacattcac attcacttcg ataacatcta
      541 aatgtgtcat attgtttact tcagtactga tcaattcttt gatttggttg taaatatctt
      601 cgatgtcttt accgtattct acgatcacat ctagatccac agctacttgt ttcttaccta
      661 cttctgtatt gatacctgct gttacgttat ctgtgttgac taatttttca gccatatttg
      721 agaaaaatcc gccatctaat gtcaatagac catctacatc tttcaatgca taaccgatga
      781 ttttttggat gactttgtct tcataagtca attcaccatt catcatgcgg ttttctcctt
      841 tttcatttgc caaaactgat ccatttgttg tattcatgtt tttgttttcc atctttgttt
      901 cctcctatga tttaaaaaat tgatcaacta ttcctgactg tttcaaataa aaaccgattc
      961 ctgcgccaat tgctgtcatg attatgatca acaatgtctt gaaaaatcca atcgttaacc
     1021 ataaaacagc tagcagtagt ccaagacccc caaagataat agggagcttg tatgctgaaa
     1081 atagttcttt cattatttgg ccctcctatt ccactcgaga gtgcttgtag tctcttgtat
     1141 cttcttgttc ttcataaccg ctgtatgtga ctgccacttt gaccgtttct tttgttccga
     1201 gtactttagt tacttcttct tcgatatctt gcatcaatac acctgttttc cctactaatg
     1261 aagaagttcg tttcaagtct ccgttgacat ggatatggat tcgatttttc gttgaacgta
     1321 tccgtacttt aggtgaatga atgaactctt cttcgtgtaa atgggaacgt accaaccctt
     1381 cgatcgcttt tttgtcgatt gttagtttgc catcttcccg tttcataacg aaagttcctc
     1441 ttgactttgg atagaaaaga acgactagca agatgataat agcaagaacg gcaaatacta
     1501 cagctgccca gaaaatatat tgttgcatgt agtaattcgt taaagtaaag aagcgaaatc
     1561 gttccaattg ttcaggcata atatacggcg cattgatcaa cgcaatcgct agaaatactg
     1621 aaagtagcag taaactcatg ataacaccga ttatttttaa ccccttattc attttccacc
     1681 tcctataaac ttatttttac cctgtaaata actctaccga caatcacagt atagatagcg
     1741 aacagttagc atagcatcag taaataaagc gctaccaaac attgctggat cataacggcg
     1801 ttttattgcc agtctgatac tgaaataaca attgcttact tattctctat ttacttttga
     1861 ttgctggcta tacttaaagg atattattca agattaaaaa ttgcaaagat tatgctcagt
     1921 tattattaca agattctatt tatttattaa attttttcgg agaattaaaa aatacaataa
     1981 ataactttag ttactttaat gcgttcttac tttattttta tattatcatt tttcttattc
     2041 acttctttgg tttatactat tcttataaat aaaaacgagg tatgtcctat gatgaaaaga
     2101 ttgtactatt cattgatcat aacgattggc tatttgattg tatcaaatct aggaaatatg
     2161 gtatttggta tcagtaaaga attcagttgg actactacat tatgggaatc tctattcttt
     2221 ttcatcttcg tatttttgtt acaaaattat cgaaaaaaat aaacatgtgc catctatctg
     2281 ttggcacatg ttttaggatt tatggtactt tttattcggc aatccatttt atttttctac
     2341 ttcttcttaa tacctctaac acttttgatt tataaactaa tccaatgcat ctacttgcgc
     2401 ttattaagct atttcagcta gatagtacca ccccatgcgt caatgcttga ttttcctttt
     2461 gtataacatt gtataagcaa taaaggacaa ataagtggtg attacaccaa accattgaat
     2521 aagaaaaatt ctgatacgta aataaacatg aacttgaaaa aattaatgaa tcggttagct
     2581 actacgtagt tgtctttttt ctcatacagt tctgttaact tacacttctc ttcgctagat
     2641 aataaaaaat cctccaactg tattgtccaa tataatccca ggcatctaaa ctagcattat
     2701 agaagagaca cacatagaat ctaaaataac tggttttaaa tgtttcttac aatcaaaaaa
     2761 agctgttaaa t
//
LOCUS       DAHUVV010000170         2730 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.175, whole genome shotgun sequence.
ACCESSION   DAHUVV010000170 DAHUVV010000000
VERSION     DAHUVV010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.175"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(41..1417)
                     /locus_tag="NK767_002816"
     CDS             complement(41..1417)
                     /locus_tag="NK767_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCH6695745.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(1545..2663)
                     /locus_tag="NK767_002817"
     CDS             complement(1545..2663)
                     /locus_tag="NK767_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCH6695746.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
BASE COUNT          853 a          557 c          438 g          882 t
ORIGIN      
        1 gtttattcaa gcaacacgtc tttcataacc aagtttccaa ttatcttctt ggctcgtcat
       61 cttcgaattc ttttctgaag tatcttcgtt cttcttcagg ttccgcttct gtttcttccg
      121 gatcatgaaa aatgacacgg attttaagga ttcgagaacc ttccatttct tcagaaacca
      181 gcgtaatatt atcgacatca aacgataact tttcgccttc atccggaatc atgcctaatg
      241 ctgtaatcaa ataaccagcc atcgtatcta catcactcat atgcagatca ctgtcaaaag
      301 cttcattgaa ctcatcaatc agcatacgtc cttggatgat atattcgtgt tcatcgattt
      361 tttcataaag attctcaact tcatctgatt catcatcgat ttctccgacg atttcttcta
      421 aaaggtcttc tagtgttgct aacccaacga cgccgccata ttcatctaat aaaatagcca
      481 tctgattctg cgtctttttc atttcatata acagatcatc aataaagacc gtttcaggaa
      541 cgaataaagg ctcctgcatg attttcttga tatctaaatt atcaaagccg aatttatggg
      601 cagcttttag taaattcttt gtatgaagga ttccgactac tttatctttg tcttcgttat
      661 aaacagggat tcttgaatag ttttcggata aaacttcatt cacattttct tccacagtgt
      721 cgttgatatc aatcataaat gcatccgttc gtgggaccat gacttctcgg gcaactttcg
      781 tatcaagaga aaaaacccct tggagcattt ctaattcttc attattcaat acgccttcgt
      841 tttcaagcat ataacgcatt tcatctctgg tcatttttga atcttcatca tcaaaagtca
      901 tcggcgtgat acgcgccaat aaacttgtgg aagcagaaag cagccagacg aagggcttag
      961 ctactacacc aatcaagcgt atcagccctg aagtgaactt tgcgacttct tctgatttat
     1021 ttaaagcaat acgtttaggg tacaattctc caaaaacaat cgatacatag gtcaaaatag
     1081 ccagcacaat gatatttgca atattttttg ccgctgtgcc gccacctaat accggagcta
     1141 accgttccga taaagtatta gctagtgaag caccagacaa aatgttgacc aaagtgattc
     1201 ctacttggat tgtcgatagg aaattatttg gattctgcaa tatttttaaa agcttttgag
     1261 atttcacatc tccttcatca gctttttgct cgattcgatt cttattgatc gaaacaacag
     1321 caatttcgga agctgctaaa aaagcattga tcaatgtcaa aacgatcaat aataaaatct
     1381 gcgctaacag cgactgactc tcagggtcag cattcataat cgcattctct ccttataaaa
     1441 ttaattttta gtttacgata gaaaaaaacg tggcactata gccacgttaa aatggtatca
     1501 caaagcaatt ttctttgcaa taaaaacaag tgtactaaaa ctacctacga ggtcgtagca
     1561 attttcttct tgttttcatt ttgcttgtct tctttgacga ttttgattat ataggaaatg
     1621 atcgtcttgc agattgcgta aaaaggaaca cctaagaaga taccaagcaa tccggcaata
     1681 tttccagcta ctagaagaac gataatgatc gttaatggat ggattttcag tgatttccca
     1741 attacattag gataaatgat attcccatcc aactgttgga cgattagcac aaccacacaa
     1801 caaagtaatg cttttaccgg atcattgaag actgttacta gaaatgcagg tgctagtcct
     1861 agataaggtc ctaaatatgg aattaggttt gtcaatcccg caatgacccc aaataagaaa
     1921 gcataatcca caccaattac catatatcct aaaaatgtga aggtaccaac gaataaacat
     1981 tcgattgctt gtccgctaat gtaattagcc aatgttttat tcagttgccc caataactcc
     2041 acaatttggt ttcttctttt ttctgggaaa aactgttgga tgtttggcac tagtttttct
     2101 ccgtctttca acatatagaa caagatgaat ggtgctgtaa tgattactac ggttgccgaa
     2161 gcgatcgttc caacgatcga acctaaacta ttggatactc cactaaggaa ttgctgaatg
     2221 attgtcccgt aagaaatgtc taattgctca aaatatttat tgatatctac ttccttgaac
     2281 aaaggcaatt cggctacctc ttttaaccag ctttctacct gtttgatgaa atcaggcata
     2341 ttagatgcca atgaggaaat ttgctgtact agacttggga tgacactaag taaaatccaa
     2401 ataattgctg caactaaaag gagcagcaca attgcgacag cccatattct tttgattttt
     2461 gctttcatca acagcttgac aataggattc aataaatagt aaagaaagcc tgcaatcagt
     2521 acaggtgcaa ataacgtgga aaaaaatgtc ccgatcggtt cgaaaataaa gtttatttct
     2581 gatgaaatca tgatcaatgc agctatgatc aataattcaa gcgaccaaaa cattagttta
     2641 gactgtctaa gtttttcaaa caatagattt cctcctttct ttcttactag tatatattat
     2701 attcgttaaa aattctttta ttcttttttt
//
LOCUS       DAHUVV010000171         2710 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.176, whole genome shotgun sequence.
ACCESSION   DAHUVV010000171 DAHUVV010000000
VERSION     DAHUVV010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.176"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            170..514
                     /locus_tag="NK767_002818"
     CDS             170..514
                     /locus_tag="NK767_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCH6695747.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="NK767_002819"
                     /pseudo
     CDS             515..934
                     /locus_tag="NK767_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1026..1376)
                     /locus_tag="NK767_002820"
     CDS             complement(1026..1376)
                     /locus_tag="NK767_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695748.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="NK767_002821"
     CDS             complement(1342..2673)
                     /locus_tag="NK767_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HCH6695749.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
BASE COUNT         1003 a          491 c          522 g          694 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gtaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 atggacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt
//
LOCUS       DAHUVV010000172         2670 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.177, whole genome shotgun sequence.
ACCESSION   DAHUVV010000172 DAHUVV010000000
VERSION     DAHUVV010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.177"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(893..1411)
                     /locus_tag="NK767_002822"
     CDS             complement(893..1411)
                     /locus_tag="NK767_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HCH6695750.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            2529..>2670
                     /locus_tag="NK767_002823"
     CDS             2529..>2670
                     /locus_tag="NK767_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6695751.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
BASE COUNT          883 a          483 c          398 g          906 t
ORIGIN      
        1 atactaaagt tatttgttct tttattaaat acacctacaa cttataaatt gatggtattt
       61 agataacacg aaagaataaa aataacagct gatagtaaag tttgttgacg attgctactc
      121 aatcgaaagt gagttagtgc gtatgaactt gcttctgaaa acttatgaaa tgaaagacct
      181 tttacatctt gcagaaatcg gtactgggat taaactaagc aataaaaact aatgttcccc
      241 tcattctttg aacttctact atttgattta tttattctga taaatgacga tttcaccttc
      301 aggatcttca aatcccgtat agaatttatt taagatctct tcagtatcag taataggata
      361 ggcgataggc cacataccct ttgatggtta gaataaactg ctcaaaatct gtatcgtatg
      421 ttggctttag catattaaca aaggctatct ccaaattata aaccataagc gttgaatcgt
      481 tcttaacaat ttcaattctt cctaacccaa agtcagaaga tataattacc ggacttgcct
      541 tacccttcat gtgggttgtt gagattccat atttaaaggg aattgtcggt actatcggcg
      601 gtctgaaaaa catcccaaaa ataaaaaaat ctttcccctg ctagtctcct aagtcccttt
      661 gtagcggata tggcaccata atctcatttt atcgaagtga gtcatggttt atctgttggc
      721 caaccaaaat tgatacaatc taacgatgca ccctctacgg cattctaggg gagtgcactt
      781 gcattcaccc aataaaaacc ggcagttagc aaagaactta tgtagtcgta tgttcttact
      841 gttaacatac aagaggtctc ctttccacaa ttgcttagca gtaaacggga atttacaaag
      901 ctaatctctt ccattttctt atctttgttc taaaagtccc aaatggagca actgtattta
      961 catgaataaa tttatatact tcccatacag ccgttttggt tgcctcgtct gcccattttc
     1021 ttttgtgtgc agtgaataga tcttcatcac tcattgaatc aatcatcatg ttaatatcat
     1081 caatattacg attgagaatt tcctctaatt ctttcaatgg tagatgtgca taggtatttg
     1141 taaaccactt atataactct cctaactgat tccatttaaa ttgatcagaa ggcgttttaa
     1201 cagataatcc atttctttca tcattttccc attttagaat taaattcgtc caaccaactt
     1261 gatatgctaa attttcagca ggtgttctat ccacttcctc aactctttta tccttcaatt
     1321 cttcaggaat agtttcaaat tctaaaatat atttagtata agtcctattt atttcttttt
     1381 ttaattcttc tttattttta tatgttctca ttatattacc tccataaatt cctaattgtc
     1441 tcctttaatt catctataat ttcatccacg atattttaat ccgttctaca atgcatatct
     1501 gatatgcatt gactgtctaa ctcatatgtg aatgatttat aaaatatccc aacgtctcat
     1561 ctaaagaaat gttgatcgtc agcaaggagc taaaaaatac gaatatattt cttttgtagt
     1621 aaaatcggac tggtttgcat atcattttct tcgtaaatat caaaatcatg ttaactacta
     1681 tcaattacta ataaaaacta ccactttaag cgttaagaac tgttaagttt agttaagtga
     1741 ggatagttca caaaatccat tgttaaactt cacgaaagtg gtaaaactca aaatgcttta
     1801 tataggatta cagcatcgct ctttcttcga ttgcgcgttg ggtaaaaaat attatgaagt
     1861 aaaaattgac gatgacacgt tccttacggc tcatcagatg aaacagctac aaaaacgaaa
     1921 tgatcaactc gaagaaaatt taattctaaa aaagcgattg ccatattcac gcctcgctcg
     1981 acgaactatt aaaggtcaag atttacctga agatcatcct ttttaggaaa ttgaagcttt
     2041 ttatgttgca caatgcattt ataatactac aatgatgttt tcaccagata ttcttctagg
     2101 gtgcggtgta atgaaacaga tacacttgca aaagaaaatt caccgcgaat ttactaaaat
     2161 tatgaataat tatgttaatc ttccgaaatt tttttccaaa attaagggat caagctggtg
     2221 ttatagattg tttaaggttg gcaaaaaaat aacttctttt taaacaatta tagaaaaccg
     2281 atactctgtt aagttggttg acacggtgtc gatttttatg actttcttat tctgaaactt
     2341 cactaacgga cagtacctaa aaagtctata gtatggacgt ttacgagtac tattttatca
     2401 aaaatgctgc tcatttcaac ttatatgttt tagtgaacta cccccactta gcttggatag
     2461 gctacaatta actagacaga aaaattaagg tgtgtagact agaagaaaac ataccaggag
     2521 gaatttttat gtctaagaga acacgaagaa ctttttcaca agaattcaag caacaaatcg
     2581 tcaatcttta cttagctgga aagccacgtg tagaaatcat tcgagaatat gaactaacgg
     2641 cttcagcatt tgacaaatgg gtaaagcaat
//
LOCUS       DAHUVV010000173         2601 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.178, whole genome shotgun sequence.
ACCESSION   DAHUVV010000173 DAHUVV010000000
VERSION     DAHUVV010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.178"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            21..623
                     /locus_tag="NK767_002824"
     CDS             21..623
                     /locus_tag="NK767_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695752.1"
                     /translation="MNIDRLKTMYDGYDGDRVIEIRFIDPRRFTVQQRNFIYALIGDI
                     FIDTGMPTDFWKEFFYFRFEGVTGRKISLKDESNTTVSDANVLANIILDFIFEHHIPF
                     KEGYEILPANQEYYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAA
                     LCRIHHTEKHKIGVINFKNKYQIKGIKLNQETIKKLRIGG"
     gene            630..1481
                     /locus_tag="NK767_002825"
     CDS             630..1481
                     /locus_tag="NK767_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HCH6695753.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            1493..1762
                     /locus_tag="NK767_002826"
     CDS             1493..1762
                     /locus_tag="NK767_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695754.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1767..1931
                     /locus_tag="NK767_002827"
     CDS             1767..1931
                     /locus_tag="NK767_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695755.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1924..2226
                     /locus_tag="NK767_002828"
     CDS             1924..2226
                     /locus_tag="NK767_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695756.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            2223..2384
                     /locus_tag="NK767_002829"
     CDS             2223..2384
                     /locus_tag="NK767_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCH6695757.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEG"
     gene            2387..>2601
                     /locus_tag="NK767_002830"
     CDS             2387..>2601
                     /locus_tag="NK767_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695758.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGL"
BASE COUNT          989 a          423 c          581 g          608 t
ORIGIN      
        1 gtgcaaccat caatgaggaa gtgaacattg atcgactaaa gacgatgtat gacggctacg
       61 atggtgatcg agttattgaa attcgtttta ttgatcctag acgtttcaca gtacagcaac
      121 gaaacttcat ctatgcactc ataggcgata ttttcatcga tacaggcatg ccaacggact
      181 tctggaagga attcttttac ttccgctttg aaggtgtcac agggcgcaaa ataagcctca
      241 aagatgaatc gaatacgact gtgagtgatg ctaacgtctt agcaaatatc atcttagatt
      301 tcatctttga acatcatatt cctttcaaag aaggctatga gattttacca gcgaatcaag
      361 aatattactt ctacaaatgc atcacaaaaa gagtctgttg catctgtggc aaaacaggag
      421 ctgatatcga tcactttgac aaagcgctag gaagacgaaa gcgcaaagaa gttgatcatt
      481 cagagtacac atttgcagca ctctgcagaa tccatcacac ggagaaacac aagataggtg
      541 tgatcaattt caaaaataaa tatcaaatca aagggatcaa gttaaaccag gaaacaatta
      601 agaaacttag aataggagga taaaaaatat tggctgacaa caaacgatac tactatttaa
      661 aactaaaaga gaactttttc gatagcgacg agatggttct tttagaaagt atgccagatg
      721 gatacattta ttctaatatt cttctcaaac tttatttaag aagtctgaaa catgaaggca
      781 aactaatgtt taatgacagg attccattca attctacaat gcttgcgact attacaagac
      841 actctgtggg agttgtagaa aaagcagtac aaatatttcg tgatttacaa cttattgagg
      901 tattagataa cggagcaatt tatatgtctg atatacaaag ttttattggg aaatcttcaa
      961 ctgaagctga taggaagaga aaatacagaa aagaaattga ggaagcaaaa cagaatttaa
     1021 taacaagtgg acaaatgtcg gacaaatgtc cggacaaaac tacaccagag ttagagatag
     1081 agttagagaa agatatagag ttagagaaag atatagagaa tgtaacgcct tcgaaaaaat
     1141 cgaaggctaa gcccgtccgt cataaatacg gagagtataa aaatgttctt ttgtcagatg
     1201 agcagatgga gaaactcaaa atagaattcc ctaatgacta ccaagagcga atagaacggc
     1261 tatctgagta ttgtgaatca tctggtaaga cttataaaaa ctatttggca actattcgaa
     1321 gctgggcaag aaaagaaaaa agtgaatcta agaacgcaag cagtggatac aagcgcacag
     1381 gaagacgaga gaagcttcct gagtgggcaa tcgaccaaga agcctatctc aagaaaaaag
     1441 cgctagaacg agctaataga caatcaaaag caccattcta agaggtggga aattgaaaat
     1501 cgattatcta gaactaatta atgaaatagc aaagtataaa actggtgagg aaatagaaat
     1561 cctgagagac gtatatgatc aactcgaaga agctggaatc gaaggaatta agaatgatcg
     1621 ttcgagttgg agtaagctca gatactattt cgcactttat atcgatacaa cacaattaag
     1681 aaatttagca tataccaaat tactatttgt tgattgtgtt aaaggcttgc aaaaacatct
     1741 taatgaactt gagcaggtgt aatcagatgg acctaaagac atttacagca cagattgaac
     1801 taatgcatca agaagcttta agacaaagtg tatcgtacga agacaagtgg ctcaacacgt
     1861 tccacggcgg acgtgagagc gcacttgatc aagtactcaa attactgaaa ggagaatgtc
     1921 gggatggata agaaagcggc aatgcagcga attatcgaat tgacttattc agaagattgg
     1981 caaaatgaca aagaagctgc ttcagaagtg atgaggcttg gaagagcgat gtgggcagac
     2041 aagagcaaca agccaagacc acgaaaaatc gcaatttggc acggtgacaa acttctagtg
     2101 atagggacag ctgaacagtt agcaagtctc acaggcttgc acgagaaaat cgtgaggaaa
     2161 agagcaaggt gtggctacac agacgctaag aagagaacgt ttagatacgt ggaggaatca
     2221 tcatgacaac agaagaagtg attcaaatgc gaattcgaag cattcaacgt gaaattgacg
     2281 atctggaacg gacaaaggca gtgatggtca atgaaacggc gaggaaggca atcgatttgc
     2341 acatagagaa tttaagaagg gaaattcgta gattggaggg gtaaggatgg ataagaaagc
     2401 agcaatgaaa cgaatcatcg aactgacaca ttctgagaat tggcaagaag acaaagaaat
     2461 agttgcagaa gtccaaaagc tcggtaaatc aatgtggact gaaaagccta aacggagaac
     2521 gccgagaaag attgcaatct ggcatggtga ccgaattcta gtaacaggta ctgctgaaca
     2581 gttatctgaa attactggtc t
//
LOCUS       DAHUVV010000174         2497 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.179, whole genome shotgun sequence.
ACCESSION   DAHUVV010000174 DAHUVV010000000
VERSION     DAHUVV010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.179"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(526..1866)
                     /gene="glnA"
                     /locus_tag="NK767_002831"
     CDS             complement(526..1866)
                     /gene="glnA"
                     /locus_tag="NK767_002831"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCH6695759.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1894..2286)
                     /locus_tag="NK767_002832"
     CDS             complement(1894..2286)
                     /locus_tag="NK767_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCH6695760.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          720 a          550 c          418 g          809 t
ORIGIN      
        1 tcgtgcctcc ttatttctaa ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
       61 caatatccat caaacatgag aagctgtttt tgaaattatt tcttgtgatt cggagttaaa
      121 tggtaggctc acaacctctg gataaacggt attcaaaagc cagtttctcg gtattaagcc
      181 aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      241 aacgtctttt tcaaaacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa
      301 gttcagccaa ttcaaaaaca agaaaagctg tttttgcttg gctattctta ttacttgttg
      361 cagggcagct ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat
      421 tagctcaaaa aacggcaaaa tatgaggaac tatttttccg ttagtttctc taatactcag
      481 gtcaaaacgc tgatttcaca gcctctaatt cacggtattc ctgttttaat aaagttctag
      541 atattgttct ctttcccact cggaaacagt ttgtctgaat gccgcccatt cgagacgttt
      601 tgcttcaaca aaatttgaga agatatgttg tcctaatgcg tcaatcatta cattatcctt
      661 acgcaattct ttgatagcat tatggatagt tgaaggtaag tcctggatat gtgcagcttc
      721 tctttcttcc tcatccatta cataaatgtt acggtcaaca gctgctggcg gtgtgatctg
      781 attacggata ccgtctagtc ctgcttgcaa caatactgcc attgccaagt atgggtttgc
      841 agatggatca actgaccgca attccaaacg tgtggataaa ccgcgagatt ctggtacacg
      901 tactaatggc gaacgattac ggccactcca tgcgacataa acaggcgctt cataacctgg
      961 aacgagacgt ttataagaat tgactgttgg attgcaaacg gctgtgtagg cacgagcatg
     1021 tttcagcaaa ccacctaaga aatgataagc tgtttcactt agttccaatt caccgtcttt
     1081 atcatagaaa acatttcctt catcattgaa caaagacata ttgcaatgca taccagaacc
     1141 attgatccca aataacggtt tcggcatgaa agtcgcgtga agtccatgct tgcgtgcaat
     1201 cgttttgaca acgagcttga aggtttggat attatcacaa gcatctacca catctgcata
     1261 tttaaagtca atttcatgct gtcctggcgc tacttcatga tgtgaagctt ccacttcaaa
     1321 gcctagactt tctaattcca acacgatgtc acggcggcaa ttttcaccta aatctgttgg
     1381 tgcaaaatca aaataaccac ctttatcatt caaggtcgtt gtgatcccac cgtcttcgtc
     1441 taatttaaat aagaagaatt ctggttcagg tcctaaattg aaagaggtaa aaccaaggtc
     1501 gcgcatatct ttcaatgctc gtttcaaatt cccgcgagga tcgccggcaa atggtgtccc
     1561 atccggatta tagatatcac aaattaaacg agcaactttt ccatgagcac tttcccacgg
     1621 gaagatcatc caagtggaga gatctggata caaatacata tcgctttctt caattcgaac
     1681 aaatccttca atcgaagaac cgtcaaacat cattttattt tctaatactt tgtcgatctg
     1741 gctcactgga acttctacat ttttgatcgt acctaagatg tctgtgaaca tcaatcgtaa
     1801 aaatcgtacg ttttcttcct caatgattcg tttgatttct tcacctgtta tctgtttctt
     1861 taccatggta tccctcttcc ttttttaatt tattcagtca aagaattgtc tttgcaatcc
     1921 ttgcgactga taatgacctg ttttgcttaa cccaccttgc gccaaaatct catcacgaaa
     1981 aatttgtcga acgtcatgat ctgtcaacgg cttcttgttt tttgcttctt gttttttcgc
     2041 ctgtgccatt tcgtatacac gtttgatccc agccatattc aatccctctg aaagatagtc
     2101 tttaatctca agcaacatat caatatcatt cagcgaatac atacgtcggt ttccttcgct
     2161 tcgttccgga tgaactaaat cttgttcttc ataatagcga atttgccgag cagacaaatc
     2221 agtcagcttc ataactgtac caattggaaa tacggacata gatcggcgca attccttttc
     2281 tcccatcaga cgtccccttt cgttttataa gttggctaaa gtgtaccaat taaaaaaaat
     2341 attgtcaact gcatatgtta ggttttctaa catgagatct ctatttttta taaaataaca
     2401 aaaaaataag aacacaaaaa aggtatctag ttgattttcg taccagatac cttttatttt
     2461 ttattatact gtactatcct ttattagtgt ttttact
//
LOCUS       DAHUVV010000175         2459 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.180, whole genome shotgun sequence.
ACCESSION   DAHUVV010000175 DAHUVV010000000
VERSION     DAHUVV010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.180"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            6..1150
                     /locus_tag="NK767_002833"
                     /pseudo
     CDS             6..1150
                     /locus_tag="NK767_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1321..1779
                     /locus_tag="NK767_002834"
     CDS             1321..1779
                     /locus_tag="NK767_002834"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCH6695761.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="NK767_002835"
     CDS             1938..2408
                     /locus_tag="NK767_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCH6695762.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          823 a          460 c          460 g          716 t
ORIGIN      
        1 gaaacatgca cattcaaaca aaattgatcc acggaggcat cagtgaagat cccacaacag
       61 gagctgtcag tgtacctatt tatcaaacat ccacttatcg gcaagatggc gtcggccagc
      121 ctaaacaata tgaatattca agatctggta atcctactcg ttttgcattg gaagaactga
      181 ttgctgattt ggaaggcggt gttcgaggat tcgctttttc ttctggtctt tctgggattc
      241 atgccgtttt ttctcttttt caagctggcg atcatatctt attaggagat gatgtctatg
      301 gaggaacatt ccgtctattt gacaaagttc ttactaaaaa tggtctcgag tacacgatca
      361 tagatacgag taatcttgat aaaatagaac aatccatcaa acccaataca aaagcacttt
      421 atcttgaaac acctagcaat ccgcttctaa agattactga tttagaaaaa agtgccactc
      481 tggctcatca acatggattg atagttattg ctgacaatac atttgctact ccttattttc
      541 aaagaccttt agatctcggt tctgatatcg ttgtccatag cggtaccaaa tatctaggcg
      601 gacacagcga tgtagtagct ggtttagtga cttctaacca aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgcctt
//
LOCUS       DAHUVV010000176         2406 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.181, whole genome shotgun sequence.
ACCESSION   DAHUVV010000176 DAHUVV010000000
VERSION     DAHUVV010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.181"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..103)
                     /locus_tag="NK767_002836"
     CDS             complement(<1..103)
                     /locus_tag="NK767_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCH6695763.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
     gene            complement(177..458)
                     /locus_tag="NK767_002837"
                     /pseudo
     CDS             complement(177..458)
                     /locus_tag="NK767_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
     gene            920..2302
                     /locus_tag="NK767_002838"
                     /pseudo
     CDS             920..2302
                     /locus_tag="NK767_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          875 a          412 c          400 g          719 t
ORIGIN      
        1 acaattcctg aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa
       61 taatgacgtc ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt
      121 cttttttctc aattttacac taaaatagat tttttggaaa actttgcaac agaaccgaaa
      181 tgagtatagt atgaattagt aatttggttt tcatctaagt ttagctttga tttactaaaa
      241 ttgatatctc gatacatgtt caatacatct aatacaaatc gagaatcttc tttcggaaat
      301 ggctcagaaa gatcactcca aatttcatca tcataatgat actcgtaacc ttctctcaat
      361 atttcgattt ttctttcata ataagatctg ttttggtcct caaatttagc caataactca
      421 aattgattta ctagtgacaa tctctgaaaa ctgtccatta aatgtttctt cctatcttgt
      481 tcattaacaa aaaattataa gcccttacaa cgaatattat atattaaatt taattgtagg
      541 tgaacacgaa ttttataaat atttatggtg ttaatgacaa atgatatccg actcctcttc
      601 atacgttctg gactatttgc taatagatta aaatttcagc atctatttct gaccataaat
      661 aaatgagctg ccgaaaagct gccaaatcag acttgaaaat aaaggatcaa aaacttttaa
      721 gtttctgacc ccgtaactca cacttcaaaa tcttacttaa aaaggagtct gaaagaaaaa
      781 aagagtattc aaaatttttt tctaactagc tacatcacat ctatgcattg acactcaaag
      841 aaccagaata agtcatcaga aagcaagggg gtgcggacat aatcctggac gaaaaaataa
      901 acttaggagg aatgtccgtg tgttttccca caaagagcgt gtaagggcaa ttcagttatt
      961 tttaaaatac gattgctctt atgctgcaac cattcgagaa ttagggtatc cgtcgattgg
     1021 tgcactacgg aaatggtata aagagtattt ggaatccgga gagctccatc aggagcaccg
     1081 aaaaaaatca aagtattctg aagaacaaaa acgaatagcg gtgaatcatt attttgaata
     1141 tggtcaatgt tatgcacgaa ccattcgcat gcttggttat cctaatagag agtccttgcg
     1201 gcagcggtgt gaagaacttg caccaggagc tagaaaactc cgaaaaagtg cggtaaagtt
     1261 aacacaagat caaaaagaag ctgtgttgaa aaaattctat aaaccgcaaa ctaataggaa
     1321 aaatttagcc gaatctggag gaattagtag agttactcta tatcaatgga aaaacacggt
     1381 tcttggaaag gattttccat tgtgtatgac tttaaaaaat caggaaaaac aaaaagagct
     1441 acttttaaag gaagtagaag acttacaaaa acaagtacac caattacagc tcgaaaaagc
     1501 attactcgaa ggagcggctg aactgctaaa aaaagaaaaa ggcgtcaatc ttctttgtct
     1561 atcaaatcaa gaaaaaacga tattgattga tgccttacga aatcagttca ccttgaaaga
     1621 gcttcttcaa cagctccagt ttcctaaaag cagctatttt tatcaaaaac aagcacttga
     1681 gaaaccagat aagtattaca aagaacgtca aattaattat cacgattttc aatcataact
     1741 tctgtgcgta tggttaccgt agaattcacc aagctttaaa aaatatgggt aaaaagcttt
     1801 ctgaaaaagt tgtacgtaga ttgatgactg aggaaaatct atttgttaaa ttttctagac
     1861 gaaaaaagta ttcttcgtat gctggggaaa tttctcctgc acaccccaat ttattaaatc
     1921 ggaattttaa agcaaagaag ccaaatgaaa aatggttaac agatattact gaatttagaa
     1981 ttctcgcagg gaaaatctac ttatcaccac ttgtcgattg ttacgatggt gctattgtta
     2041 gttggactat tggaacaaag ccaacttgct tcactctatg tctaaaaaga gttattcacc
     2101 agataattca gcgtgcgaag gtttctttgg acgattaaaa aacgagatgt tttatcaacg
     2161 tgactggaag aatacaacaa tcaatcagtt tataaacaaa ttggatgatt atattcattg
     2221 gtataataat caacgaatta agttatcttt aggaaggcta agccctcttc aatatcggaa
     2281 gaagcaaaga tatatttgtt aaaaaggtcc aaaaaaaata tccgcacccc cttttttttg
     2341 taaaaaacta ttgcacaata caagtagcct acggctatcg aaatttccat aactttcgag
     2401 caagaa
//
LOCUS       DAHUVV010000177         2397 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.182, whole genome shotgun sequence.
ACCESSION   DAHUVV010000177 DAHUVV010000000
VERSION     DAHUVV010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.182"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            206..2068
                     /locus_tag="NK767_002839"
     CDS             206..2068
                     /locus_tag="NK767_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCH6695764.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2397
                     /locus_tag="NK767_002840"
     CDS             2068..>2397
                     /locus_tag="NK767_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCH6695765.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          353 c          466 g          714 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataat
//
LOCUS       DAHUVV010000178         2381 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.183, whole genome shotgun sequence.
ACCESSION   DAHUVV010000178 DAHUVV010000000
VERSION     DAHUVV010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.183"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            38..433
                     /locus_tag="NK767_002841"
     CDS             38..433
                     /locus_tag="NK767_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695766.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            758..1375
                     /locus_tag="NK767_002842"
     CDS             758..1375
                     /locus_tag="NK767_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCH6695767.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1416..1715
                     /locus_tag="NK767_002843"
     CDS             1416..1715
                     /locus_tag="NK767_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HCH6695768.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1749..1916
                     /locus_tag="NK767_002844"
     CDS             1749..1916
                     /locus_tag="NK767_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCH6695769.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1961..>2381)
                     /locus_tag="NK767_002845"
     CDS             complement(1961..>2381)
                     /locus_tag="NK767_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCH6695770.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          842 a          351 c          453 g          735 t
ORIGIN      
        1 atatacgata aaataagtat atgaattgta aaaggacatg atgattatgg aagaaataga
       61 atggaataat ctgactcaaa ataataaaaa taattctgga tgtggaatta tctttatgtt
      121 tgttatagtt ttgggaatag tagtactttt aataactgtc aagatagtag attactttgc
      181 gaatactcct gaagaacaaa tacagagaaa acttcctgag tttgaaagag taccacctta
      241 tgatccaaga gataattcat ttcaagtaaa aaaattaaaa gagtctccaa atgaaatgtg
      301 gtttgaagtt acagttgaat caagcgactt caaagaaaaa aagaaagaat acaattatat
      361 tgtaaaggca aaagtatcaa aaagatttat ttttaaccct aaattagaaa tatatagtgt
      421 tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa agaagtcaaa ttctgatgtg
      481 aaaaataaaa tataccatgg aagacataaa agaaaaactg gataactagt ttatttaatg
      541 gcagaccgtt aaggcctgcc atttttatgt attttagcgg aataaatata atcatgcggg
      601 agtgtaaact tactgtttca aaaagtatac tttttgacat gccttgattt tctttgttta
      661 tgtatggcat aataaccaca taatacaatt tgaaacgttt ctgatttaat ctgattgttt
      721 tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg agaaaatttt tttatatccg
      781 ggtcagtagt tatgatcaat cgatcgaacg acaactgatt tccgcgaagg aattaggaat
      841 ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa gattttaaac gccctgaata
      901 tcaattaatg aaacggatgt ttcgaaaagg agatttactc tatctacaat cattagatag
      961 actaggcaga aataaacaaa tgattttaga agaatggcga gaattaacta aggaaaagca
     1021 gattgatatt tctgtgttag acatgcctct gttagatact gctagttaca agcaactaga
     1081 tgggttagag acgcttatct ctgacttaat tctgcaatta ctcagctata tggccgaaga
     1141 cgagcggaaa cgaattaata gccggcaaag agaaggcgta gaagctgcga aacaaaaggg
     1201 tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa tttcctgcga tctattctga
     1261 ctggaaagca ggaaagataa ctgcagtcta tgctatggat caattgggca tgaaacgaaa
     1321 taccttttat cgacgggtga aagaatatga acataccttg caccactttt cttgatagat
     1381 ataacagcta ttagacggga agggagagtt tgaaaatgga cttaacgaat caaagggtcg
     1441 tttttactgg aacgctgcag caaatgacac gaacccaagc catcggtttg gttcactctt
     1501 taaacggcca ttgtcaatca agtgttactt caaaaacaaa tttcctggtt tgtggtcagt
     1561 attctaagtc gttgtttgat gattctctct atacaaaaaa ggaacaaacc gcgcgggact
     1621 tgattgcgtt aggtcatgag attaccattt tatcagaagt cgaattttat gcgttaatta
     1681 gtcaacagtt gaaagaatgg caacggtatt tataatcgtt agtaaaattt acaaggagga
     1741 aaatttttat gaagcgaaaa tcttatgatc gttggggcat tgaaataact agttttaatc
     1801 gaaatattgc ttttcacgtg gagtgtgact gtggggaatt agcaaaaagt gtttatcaaa
     1861 aaaattactt ttactgtcct aaatgccatc gccgatacgg ggtacatgga acatagaaac
     1921 gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt ctacaaatca ttcgctttct
     1981 ttattttata aatagttggt cgagagatcc cgttttcttt tgcaatctct gcaactggaa
     2041 tgccttgctt taacatgtcc accacggttt taaaaactaa gcgtttttgc ggatctgttg
     2101 agttaataga atactcgatt ggtcgtccct tatatcggcc ggctttttga gccaataata
     2161 ctccttgccg ctgacgttca cgaattttct cacgctcgtt ttgtgcaata taacttagca
     2221 gtgataaaaa catatcgaac atagcggtat ccaggagttc atttccagta ttaaaatcta
     2281 aaaactgtgc atctaatatt tttaacgcta cttttttctg tttcataaaa gcaactgtat
     2341 tttttatatc ttcataatct cttcctaaac gatctagtga t
//
LOCUS       DAHUVV010000179         2351 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.184, whole genome shotgun sequence.
ACCESSION   DAHUVV010000179 DAHUVV010000000
VERSION     DAHUVV010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.184"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..544
                     /locus_tag="NK767_002846"
     CDS             <1..544
                     /locus_tag="NK767_002846"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCH6695771.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="NK767_002847"
     CDS             complement(1017..1589)
                     /locus_tag="NK767_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCH6695772.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /locus_tag="NK767_002848"
     CDS             complement(1605..2210)
                     /locus_tag="NK767_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HCH6695773.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          741 a          468 c          359 g          783 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc agttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta g
//
LOCUS       DAHUVV010000180         2341 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.185, whole genome shotgun sequence.
ACCESSION   DAHUVV010000180 DAHUVV010000000
VERSION     DAHUVV010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.185"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            134..346
                     /locus_tag="NK767_002849"
     CDS             134..346
                     /locus_tag="NK767_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695774.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            508..1026
                     /locus_tag="NK767_002850"
     CDS             508..1026
                     /locus_tag="NK767_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCH6695775.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1033..1545
                     /locus_tag="NK767_002851"
     CDS             1033..1545
                     /locus_tag="NK767_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695776.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1816..>2341
                     /locus_tag="NK767_002852"
     CDS             1816..>2341
                     /locus_tag="NK767_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCH6695777.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIV"
BASE COUNT          892 a          298 c          385 g          766 t
ORIGIN      
        1 taaaaacact atatattccg aaaagtgagt atctccattc ataaaataat caacatattt
       61 aaaaaatcta attagctttt ttagtcaatc taaagttctt tattctatga tataatgtta
      121 ctgtagaaag tttgtggcgg tttggcaaat ccaattgaag gagcgtggtg cctatgggct
      181 tattagctta tcggacgtac tctgtagaaa ggaaatgcct gatttgttct cattggatca
      241 aatcattgct attatcggag cagcagcagt agttattact gccgtagcga aagctagcga
      301 ttcgatttta gcttggttaa agttcaaaaa agaacaaaaa aaatagagcc gtctcaactt
      361 ttggcgaggt agacgagctc taaagcttta tttacatgcc atccgctttg aacggactac
      421 actgggcatg tgacagcatg ccctttcatt tatattatat ctcattcttt tatataatga
      481 aagtaaataa atcatggagg ataaaatatg gataaattta atagattaac attaataaat
      541 cagtttgaaa ttttaaaagc tttaaatcct aacgaagaaa aatactatgc tgaaaaaata
      601 gaaatactaa cagaaggtta cgaatatcac tatagtgaaa tatttgaaaa tatttctgat
      661 ccattgccag aagaagattc gcggtttgtt ctagacatat taaatatgta tagagatatt
      721 acttttagta aaaataaatt aaaagatata aattcaattg ataattatta tatacaattt
      781 aaaggttttg actttaatag ctctaccgaa tttaagttag cactttatgc acaatttttt
      841 attgaaaaat taaataggtt tcaagaaata gttgaagata gtgattttaa cacttttaat
      901 agccataaac cgatgagagg tacttatata aaatttctcg aaaattataa tgatttaaaa
      961 tctaccaata attatcaatt tggaaacttg tcttatgaga tgataagtaa agttctaagt
     1021 ttgtgatgat aaatgaaatt aaatagtata aatgatttat ttgctattat aggtgttatt
     1081 gctaacatac ttttttcgtg tttgaatttt agatttactc aaaagaacaa aaagaacaaa
     1141 ctcagtatag aacaaatgaa aaaagacatt gaaactgatt taaaaaatga ccataaagaa
     1201 agaagaaaaa ttgaagaata tacttcttta atggaaaagt gtaaagcaac tgatgcaatt
     1261 tttagctttt taagaaaaag tgatgagata atcacacgtg aaaatataga tttagaaaat
     1321 gagattagta aacttgaatc tatcttttcc gatataaatt ctcttaagta tttaatcgtc
     1381 gaagaaaatt cggatagctt tttagaattt gaaaaatatc taacagatct tatagataat
     1441 agaaataatg aattaatgag acaaaacatt gcaagattcc ttactaaatt agtttctgaa
     1501 tggaaaagta ttatttctca aaatatggat aaattaagat attaaggatg ttagttttaa
     1561 aataaactaa tgtccttatt tttgtgttca catttctgtt acctaagaaa tacttaaagg
     1621 aggtgatgta gccagcaacg actacaactt gaacccgcta tctacagttt atttgttgtt
     1681 tcttttgggt atactctaat ggtacaaatc catatttgct taaagcgttg atttttcagc
     1741 ggtttttctt tattgcaaag tgtaccctaa gacctgtata ctaatagtaa atagattgga
     1801 taggaatgat ggcaaatgta tttagcttat ttaagagttt cttccgacga tcaaagttta
     1861 gctcggcaac ataagctaat tgaaaattgg tcagtcaaaa ataatattgc tgaagacgat
     1921 ttaaaaattt tcgaggaaaa agtctctgga aaaaatattg aaaacagacc agagctgcaa
     1981 gagcttattt ctttcatacg tgaacgagat acgattatta ttcctagtct tgatcgatta
     2041 gcaagaaata gtaaagatac aaaagactta cttcaattgt ttcgtagtaa aggtgcttct
     2101 attgaaatat tagacctacc gtcgtttaat ggggtaactg accctgcatt aagagacttg
     2161 ctaacaaatt tagtcataga agtgtttagc tatgttgctg aaaacgaacg gaaaaaaatc
     2221 aaagagcgtc aaaaacaagg cattgaaatt gcaaagaaaa atggtcgtta tactggtaaa
     2281 aagattcaat accatgccaa tgcaacagga aaaaacaaat tgatttacga tgaaattgtt
     2341 g
//
LOCUS       DAHUVV010000181         2311 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.186, whole genome shotgun sequence.
ACCESSION   DAHUVV010000181 DAHUVV010000000
VERSION     DAHUVV010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.186"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            25..702
                     /locus_tag="NK767_002853"
     CDS             25..702
                     /locus_tag="NK767_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCH6695778.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
     gene            complement(839..1255)
                     /locus_tag="NK767_002854"
     CDS             complement(839..1255)
                     /locus_tag="NK767_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695779.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1258..1542)
                     /locus_tag="NK767_002855"
     CDS             complement(1258..1542)
                     /locus_tag="NK767_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695780.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(2242..>2311)
                     /locus_tag="NK767_002856"
     CDS             complement(2242..>2311)
                     /locus_tag="NK767_002856"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCH6695781.1"
                     /translation="CSLLVGLLSALFSYWLNNRNKK"
BASE COUNT          748 a          355 c          416 g          792 t
ORIGIN      
        1 ttaacagtca aatatcaaaa tgaattgaat ttagtaccgc tgaaaaattt caatgctaag
       61 cagatggatt tattttttgc attatgcgct cgtatgaaag atcgtggttt gaaaaaagtt
      121 agattttctt tcgaggaatt aaaagagtta agtgattata agatgactgc tacaaaagcc
      181 tttgtcggtg atttagaaaa gctctataaa gatatgctta atcttactta ccgtactgaa
      241 gatgaggatt ctattcgcta ctttgttttg tttactggtt ttgaaatcaa taaaacaaaa
      301 aagtttgttg aagtcagtgt taatccggac ctagaatata ttattaatgg tttaacagct
      361 gaatttagta gatttgaatt atctgctttc acgtcgttgc gttctacgta tgcaaaaact
      421 ctattccgac tgcttatgca gtaccgttct actggttatt atgtagttcg tattgaagag
      481 tttaggagct taatggatat cccagaatac tatcaaatgg gtaacattga tcaaaaagta
      541 ttaaatcctg cattacgaga actaaaaaaa tacttcaatg atcttaaagt cacaaaaata
      601 aaagcaaaaa agggcaataa aatcgccaaa ttagaattta ctttttcaag cctaacgtca
      661 gatttgccaa agatttctct gcgtaactgg ttagaagaat aaataaaaaa gaactcgccc
      721 cccttacatt aagcaagtag acaagtccct tacaaccgac taagattgct agtacattct
      781 agcatattgt atggggcttc taaataaaaa acagatagga ataactccta tctgtttttt
      841 atcgttttag aaaatttgtt gggtcgaaat ttctagaagt gactgtttga ctggtttctt
      901 tttgatgatt ctttggtcga tttcgatttg atggatttag tttagcttcg attttttctt
      961 tcacttcttg atatacttta gaatttgtgc caataagaac gcctgtatct tttagatata
     1021 aatcagcaaa ttctgcgatg atttcagtca tgatggaaat ttcttttccc atagcattta
     1081 gttttgcctg attgctggta gtggtcgtgt tggtatcggg ttcataactc atacataaaa
     1141 agcgaatggc ttccgagtag ttattgatat ctggattttt ttcgatgatg gaatctaaaa
     1201 cgtcaacatg actagggtac aaagtaacat gtttttggac agaagttttt ctcatggtta
     1261 tatcattcct ccgataagca tttcaatgat ttgattattt gtgtagacat actcgcttaa
     1321 tatttgagta ttttctgttt ggatagcaat aaatttatcg agcaataaat cgaattggtc
     1381 ttttttgtaa gtcgtaagtt gacggtcaaa atcactttcg attaaaactt ttagatattt
     1441 atttcgactc catttttctc ctgttttatt cgtgagttct tggcaacgtt tgtctattgt
     1501 tgcaacaaac tttggatcaa tattccgaac taaaatgtcc ataaaaaatc tccttttgca
     1561 attatgtata atttatgtat accatacaaa tactatattt gtatagctgt tttttgccaa
     1621 aatgctatac ataagctacc cacgatggcg aaaaagtgat atcaaatttt tttctgagaa
     1681 acgtgatagg cacagcctat ctacccactt tttttagaaa gtgggtacac gtttccttat
     1741 gctctttaaa attcgggggg gagtgctgca cgtgtaatcg ataaccaaat ttaacagacg
     1801 aaaaattgtt ggaatataaa aatatgacac ccgaaagaaa acgatcgttg gaaaaattaa
     1861 tgcaactagt gaacgaagac ggcggaattt cagacgcagc ttttgacgca tggagcgaat
     1921 ttcatggaga atataattaa gtgggtttaa atggctctaa aatcgcttct aagagttttt
     1981 tagcttggac caatgaaata caccaaaaac taaataaaac gtcttaaatc gaaaaatacg
     2041 agctctcaga gcgactttat ttgtaaatgt tgcatataag aaataattac ggattttaga
     2101 cttttgtagg atatttttga aataaatatt tatttctgtt ataatttgat tgcaaataag
     2161 tgaacacgaa acacacgaaa tccccagaga ggtcggattc tctggggctt tttttgtggg
     2221 ctgagtcgcc ttaattaagt tttacttctt gtttcggtta ttcaaccagt aactgaacaa
     2281 agcgcttaaa agcccaacaa gaagggagca a
//
LOCUS       DAHUVV010000182         2284 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.187, whole genome shotgun sequence.
ACCESSION   DAHUVV010000182 DAHUVV010000000
VERSION     DAHUVV010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.187"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            139..2136
                     /locus_tag="NK767_002857"
     CDS             139..2136
                     /locus_tag="NK767_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCH6695782.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          782 a          413 c          507 g          582 t
ORIGIN      
        1 aaaaaaatat taaatacttc ttggttagaa aaaataagaa aaggtgcaca agaacctaag
       61 aacaaaacgt ttcgccggtc ccatgttcct tttcgcttaa attttctttt tttcattata
      121 tttacgttat ttgttgcgtt gattgctaga ttgggttatt tgcagatcgt gaacggcgaa
      181 gagatggagg cgcgagtgaa gtctacttct acgattacgg tccaagaaag tactcctcgc
      241 ggaatgatct acgactccac aggaaaagct ttagttacga ataaagcgaa tcaggccatc
      301 acatatacga gaggaacaca agttacggct aaagatttgc ttgaaatcgc aacgaagctg
      361 aactcgctga tcgatgttcc ggtagatgaa aatctgacag atagagataa aaaagatttt
      421 tggctggctg atcccaaaca tttgaaagaa gccacaaaaa gactttcggc aaaagaaaag
      481 caattaggga acagcgaaca atatagtgcg atggtcaata aagtcacagc tgatgagatt
      541 aatttaaacg aggagcaatt aaaaattgct accatcttta aacggatgaa tagtgcatat
      601 gaattaaaca ctgtctatat caaaaactct ggtgttactg atcaagagtt agctgttgtg
      661 gcagaacgcg cttctgaact tccaggagtt tcgactggca cagactggac acgagaatat
      721 aatgcagcaa gttcactgaa aagtattctt ggcagtgtga cgactgaaaa gcaaggactt
      781 ccagcagatg aagcagagaa atacctagca aaaggttatt ctcggaatga ccgtgtagga
      841 caaagttatt tggaaaaaca atatgaagat gtcctgcaag gaaccaaaac ccaatatgaa
      901 gtttcccttg ataatgaagg aaatgtttcg aatcaaaaag aaatcttctc tggggaaaaa
      961 ggttctaatt taatgttatc tatgaatgca gaattccaat caaaagtaga agaaattttg
     1021 aaaaggaatt atcaaacatt gatcaacaac ggcaaagcac agtattcgcc tggtgcttat
     1081 gcagtggcta tgaatcctca gacaggagaa gtgctagcaa tgactggttt cagtcatgaa
     1141 caaggttcca aagaaatcac cgagaatgct ttaggcacga ttactagcgc ctttgatcca
     1201 gggtctgtag tcaaggctgg tactttgaca gcaggctggg caagtaacgc gatcagcgga
     1261 aatcaagttt tgattgatga gccgattcgt ctgcaaggtg cttcagaaaa aagctctgtc
     1321 tttaatcgaa gcggacaagt tgctcttgat gcagtcaaag catcggaatt gtcttctaat
     1381 acgtatatga tcaaagtggc gttaaagatg ttaggactag actatacacc tggaatggga
     1441 ctacctagtt tagatgaaga agctaaagct tatcagcaat tgagagactc tttcaaagag
     1501 tttggtcttg gtacaacgac tgggatcgat ttgccgaatg aatctcctgg gatctcacgt
     1561 tctgttgatt atatgaagaa gttcaatgcg gataatggca aagaatggta tgctccaggt
     1621 aactttaccg atttggcatt tggacagttt gacacatata caccaatcca attagctcaa
     1681 tatgcttcaa cagtcgctaa tggcgggaaa cgagtacagc cgcacctagt caaagcgatc
     1741 tatggcaatg acgaaaatgg aaacttgggt gaagtgaaga aagaaattgg cacaacagta
     1801 gaaaatacgg tcaatatttc tgcagaaaat atggcgatcc tccgtgaagg attccaccaa
     1861 gttgtccatg gtaccgatcc ttacacaaca gccaaaccat tagccagtgc gaaaatggac
     1921 ctttccgcaa aaacagggac tgcagaaacg gttgcggaag gtcatccaga tattgcgaca
     1981 gtcaatagta atatcgtggc atacggacca acagacaatc cggaaattgc catcagtgtt
     2041 gtcttgccga acttattaga cgaacaagac cacataaact tgacgattgc taaagaaata
     2101 atggacgcgt attatgatat gttcatggca aaataaaaaa gaggtcgtga aaaagccgct
     2161 tagtttcgag tatgaagaaa aattttgtga aaatagcttc tcacattttt gcttaaattt
     2221 gacttcatac cgagaaactg gcttttgaac accgtttact cggcaaaaaa ggttgtgaca
     2281 aaaa
//
LOCUS       DAHUVV010000183         2269 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.188, whole genome shotgun sequence.
ACCESSION   DAHUVV010000183 DAHUVV010000000
VERSION     DAHUVV010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.188"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..914)
                     /locus_tag="NK767_002858"
     CDS             complement(<1..914)
                     /locus_tag="NK767_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCH6695783.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="NK767_002859"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="NK767_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCH6695784.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="NK767_002860"
     CDS             2006..2176
                     /locus_tag="NK767_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695785.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT          767 a          390 c          427 g          685 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaa
//
LOCUS       DAHUVV010000184         2268 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.189, whole genome shotgun sequence.
ACCESSION   DAHUVV010000184 DAHUVV010000000
VERSION     DAHUVV010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.189"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(1015..1305)
                     /locus_tag="NK767_002861"
     CDS             complement(1015..1305)
                     /locus_tag="NK767_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695786.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1513..2204)
                     /locus_tag="NK767_002862"
                     /pseudo
     CDS             complement(1513..2204)
                     /locus_tag="NK767_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          647 a          432 c          331 g          858 t
ORIGIN      
        1 catacttgca aagtaatact acaattccta acataatcgt agaatttaat aatttccata
       61 gcataaagtg tttagaaaag aacattaaga atagttctat aataattctt gaactccatg
      121 tcttataatg ttctatcgaa gcacccaaat agccaagttc atctaatcta cttgcataaa
      181 ctacgtcatc cgctctcata gtcggcatat acatattaaa gagtaaaaag ataccccaaa
      241 tccctattag taacaagatc agttctttgt tattcgttat ttttctatta tgtttgttca
      301 tttttatact ccttcttata tttcctaata gagtataaca tagctttaag cattacttaa
      361 agataactta aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa
      421 gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta
      481 aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc
      541 atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt
      601 taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact
      661 tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa
      721 aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat
      781 ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata
      841 tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt
      901 atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc
      961 atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt
     1021 tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac
     1081 aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc tattattata
     1141 ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg
     1201 ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac
     1261 ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat
     1321 accattatac gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact
     1381 ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt
     1441 actaaaaaaa agaggcagca gtatagatta ctgttgcttc tttactaatt taggtttact
     1501 tgttaagaaa gattatttac gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt
     1561 atcttccact tcatcttttg ctttttctaa cttttgaata tattgtttca agcttgagtt
     1621 ttcagcagtt gcttcgtcta atttcttttg tgtatctgtt aactgatttc tacatttgtt
     1681 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1741 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1801 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1861 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1921 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1981 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct
     2041 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2101 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2161 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2221 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtca
//
LOCUS       DAHUVV010000185         2035 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.190, whole genome shotgun sequence.
ACCESSION   DAHUVV010000185 DAHUVV010000000
VERSION     DAHUVV010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.190"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(547..912)
                     /locus_tag="NK767_002863"
     CDS             complement(547..912)
                     /locus_tag="NK767_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695787.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(988..1233)
                     /locus_tag="NK767_002864"
     CDS             complement(988..1233)
                     /locus_tag="NK767_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6695788.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1303..1557)
                     /locus_tag="NK767_002865"
     CDS             complement(1303..1557)
                     /locus_tag="NK767_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695789.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1721..2026)
                     /locus_tag="NK767_002866"
     CDS             complement(1721..2026)
                     /locus_tag="NK767_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695790.1"
                     /translation="MPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVITLLLLV
                     MMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINPLIFSR"
BASE COUNT          752 a          353 c          364 g          566 t
ORIGIN      
        1 acaaaaaaag aaagcgttat tattttagtc tattttcagg gggggaacct tatgataaaa
       61 aaactatttt tactatcaat attaacgcta gcaatggcag gttgttctga ctctggctta
      121 gcaactgaac tgatcacaaa atcaactgaa gccacgacag aggagttaaa atgtggaaac
      181 gatcacagtg actacagagg cttaaatcga cgatgcatgg gaatattagg cagaagcaat
      241 tgtaagcatc gatgtagtaa tcgaagaagg tgtagaagaa gcaacagaaa aatcaaaaga
      301 aagatcaaaa gaagcaatct gtgctgtgca tcgtatacag acgtcgaaga tgcggtactt
      361 ttaaaccacg tcaggagatg ttcatttatg tctagggaag ttggcgatga accaaaacta
      421 gagtacgctt ttagttttgc acgtgaggat gaagataacc ctaaaactta cgacatttta
      481 gcttgatcta agatggttaa agctttttaa tgaagtaaga ggagatagaa ggcttttcta
      541 gttatattag gtaagataaa ttcctttttg ctccaaattt gctaaacatt ctgctagata
      601 aacttcatcg tgttccggat aaatcggcac gttgcgctct acagtaggta aatcaaaaaa
      661 tgcttcgcat gatttgccac gatacatagg atttttccac tctggtgctg ggctgtaacc
      721 acgtttttcc atttcatcca tgacaagctg atgatattgg aataatctat aaggagaata
      781 gtgaaaaaca taatccacag tagcatgttt ctttccccag ccatttcctc ttaatgcaca
      841 gcattcccga tgttgtccta aaagctgttg acgaggaagc tttgaaatca atttttcatg
      901 ccataatcgc atagaaatgt ctcctttata tatagattgt taaccttaga cgttaaatga
      961 aaaaaccagc accaaggctg gaaaaaatca cttcagtttg cttgctgttt ctgagcgacc
     1021 aattaggtaa tcaacggaaa tatcgtaaaa gtcagcgata tagattaatt caatgatgct
     1081 tggaatttct tctccttctt cgtacttacg atatttgaat aaaggaatac ttaacgcgct
     1141 agaaacagct acttgagata aagatcggcc agttcttaag aaattcagac gacaacgtaa
     1201 agtatccagt aaaatagctt cttcgatact catttaatca cctttcatta tagtagaata
     1261 gttataatga ctatagaaag ctacacatgc aatcaatagt actcaatgtt ttttatttaa
     1321 acgtatcaaa taaagaatga cgatcaataa agacaaaacc agtcctgata attcttctat
     1381 aaaaacacta tcgggttcaa agtagttgta attaaaaact ttccacataa agtagatagc
     1441 cggaagagtc aaaatagcta aataaaaccg ttctttgttc caattcataa gataaacaat
     1501 acttgcgacc agtgcgaccg caggcaccca aaacaaaact ctcaccaacg taatcatgta
     1561 acggactcct caacgattca tataataaaa gataacatgt tattgcatac atcacaaatg
     1621 aaatgctagg aaaataaaag aagaatagat caatgaaaaa tgaaaaagca gaacagtttt
     1681 ctgctgataa gcaggaactg ttccgctgaa tgtgtcttat ctatcgagaa aaaatcaatg
     1741 gattgatatc taagtaattc aatacatata aaaagaggac gatcgcattc actaaaaagg
     1801 aaagaatgat gatcccatag cgaatggaaa catatttaac aaatactttt gtttgaatac
     1861 gggcatatct ttttccttta tagctgagca tcatgacaag aagcaaaagg gtaatcacac
     1921 cttgaaaaat cattccaata aacagaattc ctgaggtttg ataatttaat tggagaaaat
     1981 aagctgctgc cgcaactaaa tcgaataaaa tcgcaataaa tggcaaaata atcac
//
LOCUS       DAHUVV010000186         2020 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.191, whole genome shotgun sequence.
ACCESSION   DAHUVV010000186 DAHUVV010000000
VERSION     DAHUVV010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.191"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            100..570
                     /locus_tag="NK767_002867"
     CDS             100..570
                     /locus_tag="NK767_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HCH6695791.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="NK767_002868"
     CDS             792..1895
                     /locus_tag="NK767_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCH6695792.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          546 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatact
//
LOCUS       DAHUVV010000187         2010 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.192, whole genome shotgun sequence.
ACCESSION   DAHUVV010000187 DAHUVV010000000
VERSION     DAHUVV010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.192"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            91..420
                     /locus_tag="NK767_002869"
     CDS             91..420
                     /locus_tag="NK767_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCH6695793.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            410..697
                     /locus_tag="NK767_002870"
     CDS             410..697
                     /locus_tag="NK767_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCH6695794.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            981..1838
                     /locus_tag="NK767_002871"
     CDS             981..1838
                     /locus_tag="NK767_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCH6695795.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            1839..>2010
                     /locus_tag="NK767_002872"
     CDS             1839..>2010
                     /locus_tag="NK767_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695796.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQ"
BASE COUNT          748 a          269 c          396 g          597 t
ORIGIN      
        1 acgagtcaca gtaattactt gtaaatacat tatctcgtgg tataatgtat ttactaagta
       61 attacatact gttagaaatg agggatttaa atgattacaa aaacaagaaa acaaggcaat
      121 tcaatcatgt tgacagtacc taaagatttt aatgttccta atggagtcga agttgaagca
      181 aagttggtag agaatggtat tctttatgaa tttgttgaac cacaaaaaga attttatgat
      241 tttagcgaag atattttgtc tgatataatt gcagaaggct acgataaaga tgagattctt
      301 gtagaattta agaatagaaa aaacaaaatg cattcttcct ttagagatat tgctgaagat
      361 acacttacta attctaaagt aatgactaaa gaggagttgg caacagaaat tggcttatga
      421 aatcgttcct tcaaaacatg tcattaagta tttaaaaaag ctaaaagaga agccattgaa
      481 agaaaaattt ttgaatttaa tttatgatga aattgcgatt gatccatata agggtgaaca
      541 aaaaactggg gatttatcag gtatttggtc tagtgggttc aaatatgcgg gcacaacata
      601 tagagtagct tacgagatta tagaaaacaa ggttattcca gtattgttat gtggaacgca
      661 tgaaaacttt tatgaacagt tgaaaaaatt aatctagtga tttgttgtcg ttcttaagaa
      721 ggacggcttc tttttatcga aaatctgatt cgtctacatg actaaagtcg cgagtattct
      781 ctgcttctcg ataaattaaa taacgtcatt actttgagga aagctataaa tcaatatatt
      841 tcagcttttc cttatttatg tactaccttt tacttattaa ttataaggta gtacatatat
      901 atcttttttt ggtagtaact aaaaataaat atgttataat agtatcaaat gaatattgct
      961 aaaagaaaag aggtaggaac gtggtagcga agaaatatgt aatcggtaat tttaaaggtg
     1021 gcgtaggtaa aagtacgtgc gcgcaaatgt tagggtttga atcagcagtt aatgaaggaa
     1081 aaagaacctt gataattgac ttggatatgc aaggaaatac ttctgatgtg atgaacttaa
     1141 cgcatatgaa cttcactaac gaagaaggag gcggagaagg tgaaccgatt gtttacgaaa
     1201 atactgtgac tgacgtgttg ataaacaatc ttccacctaa agaggctgtc tataaagtaa
     1261 tcaataacct ctatatactt ccagcagaca tgtcctttga aatgtatgat gattggatca
     1321 aagaccaata taatgattca ttatcccaat tcaaatatat gaaagaaaag ctagaaccat
     1381 tatttgatga gtttgacgtc atctatttag atgtcccacc ttctatttct gtatatagta
     1441 aatccgcgat gtacattgcg gattgggcaa tcgtcgtctt acagacccaa gtaaaatcga
     1501 tgagaaatgc gatgcaatat cttgagtata tggacttttt tgtgaaagaa tttgatacaa
     1561 atttatatgt tgctggtgtt attcctttca tgttggaaag cggtgacgca gtagatcagg
     1621 aaatgtataa acaagcccaa gagatttatg gggaacattt gattaaaaat gttgtactca
     1681 aaaatgcgcg cttaaaacga tacgacggat caggtattac gatggagaag acaaagaaag
     1741 gtctcttaaa acaatgggat cgcaaaagcc acgaattatt tatcagtatt ttgaaagaat
     1801 tggaagaaca cgagaattgg tatgcggagg tggagtaatt ggctgacatc aaaggacttg
     1861 atatttttgg gaacaacaaa ggactcgcca aaataaaaaa agaagcagaa gcaaagaaaa
     1921 agcagcaagc aattacacca tctgaaaata agcaattaga agtgcaccaa gttcaagaaa
     1981 taaaaacttc ctccaatgtt gaaagacaga
//
LOCUS       DAHUVV010000188         1999 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.193, whole genome shotgun sequence.
ACCESSION   DAHUVV010000188 DAHUVV010000000
VERSION     DAHUVV010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.193"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(42..1469)
                     /locus_tag="NK767_002873"
     CDS             complement(42..1469)
                     /locus_tag="NK767_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCH6695797.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            complement(1462..>1999)
                     /locus_tag="NK767_002874"
     CDS             complement(1462..>1999)
                     /locus_tag="NK767_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCH6695798.1"
                     /translation="HDYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHG
                     SQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGP
                     DLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSD
                     KLLNRIDKEILKVGNIDE"
BASE COUNT          753 a          325 c          240 g          681 t
ORIGIN      
        1 tcaaatgtaa aggagtccaa gcaatgaaag ctgtcttcat attaattctc cttttttaaa
       61 tacaagtaag aaatgcttcc caaataatac cagaaaacaa ttgaaatgac atcgcgctta
      121 ttgaaaagta catgagcctc caccatttca tctataaaaa tagatagtat aaaaattaaa
      181 acaataccaa gaaacgcata attcacttta cttctaaaaa atgttttgag taaactaaag
      241 atatttaaca catagaaagc atagatacat ataaaagcaa ttataccact tgatacaagt
      301 acttgaagaa atccattatg catgtttcca tgtgctcttt ttagttcatt cacatctgta
      361 gtatcaagat ctactttttt caaaaatttt gcatcgctat tcccatagaa atcggtctct
      421 ccataaccaa ataacaaagt tggtcttata actttaagcg cagctttcca aatcgtaaaa
      481 cgaccagctg aaatttcgga gttatcacta gaatgctgta atattacttc tttttctgga
      541 agtggagttt tatctgattc atttgactct ataacttcta cttttctatc aatcgataca
      601 ggaattgttg ctaaaaaatc cttagcataa ttagtaccaa aagaaaaata taccaataga
      661 actaataata tgcttaccat ccatttgatt tttacaggta gtacttctct aatgctcttt
      721 ctaaattttg aattggcaaa caacaaaaga aataatacta aaaaagaaca aattactacc
      781 tgggcacctc tagacgaaga aagtgtaata taaaatattt gtacgataaa attcatcata
      841 taaaaaacac gaatcgtttt tgttattttt gaaattaata cactaattaa acttaaaata
      901 atagaagtat atccaaacat agctccaaca tttggacttg tatagacacc aaataaccga
      961 tgttcaataa aaccttgtct tgcaagtaat cctggcgttc ctgttgggac atgataagaa
     1021 atcaaaaaaa taaattgata gatagatatt agcgcagcta aaaaaattat gataatgaaa
     1081 acattattaa aatacaccat ttttttcttt ttttcctctt cactttgtga aaagtctgca
     1141 ggaaaaatta ctatcaaagt aatcagcaaa tatccaaaat tataaataga tggaattaaa
     1201 aaattttctc tgttaatgat tatagttaag ataaaactaa tcattgctcc aatcagtagc
     1261 caagaaaaac gcattttaaa aaaatttttc tttataaaaa cctcatacaa aactataatt
     1321 ccaccccaag caaaagctat tttcatcggg atattagtta cattaaaagt actaggcata
     1381 aaataaagca gacaaatgat taaatttatt actcgaaacg catctcgact taataaactc
     1441 ttctctaaaa caggtactat tctattcatc tatatttcca actttcaaaa tttctttatc
     1501 aattctattt aatagtttat cagagtaatc cgatttaact tttcttacaa ataatttttc
     1561 actttctttt atttgatcta tatccctttc atcaaaccat ttgttcatgg aaggatcaat
     1621 aatatgaata tttgctaatt tcgaagtttg taaagtagtt tcttcaatat ttttcaattc
     1681 aagtggtaaa tctggtcctc caataacatt tttatttttg aacgaggaat taaaaaaaag
     1741 tgtatgaaaa aatttttcat ctggggcaaa catacctatt ttaaaattca ggtagtcttg
     1801 ttgattttgt tcatacattt gtattaattc tgttaggcat tctttcgaca aagcccacca
     1861 ttgagaacca tgatacaaat caaatttatg gtgtctaatt tttgggggaa gtttaatcat
     1921 cctcagacaa cgattaatta tagctctaaa aattttaaat gcagttgtgt tattcctatt
     1981 aattctagga taatcatgt
//
LOCUS       DAHUVV010000189         1933 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.194, whole genome shotgun sequence.
ACCESSION   DAHUVV010000189 DAHUVV010000000
VERSION     DAHUVV010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.194"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            99..272
                     /locus_tag="NK767_002875"
     CDS             99..272
                     /locus_tag="NK767_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695799.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILHLNNS"
     gene            complement(460..1779)
                     /locus_tag="NK767_002876"
     CDS             complement(460..1779)
                     /locus_tag="NK767_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HCH6695800.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          593 a          354 c          379 g          607 t
ORIGIN      
        1 gcaaaacatt tttaacgtaa gcgccccata gaacagtacc ttactaacaa atgcacctca
       61 agatatactt gagctatcaa gaagtctgga ggtcttctat gtataaatta aatgagattg
      121 ttcctgtcca attaggaaac gaaaagagtg caccagaaaa aggtaaaccc gcggctgcca
      181 agttagcgat gcgcatcaaa agagagaatg gttctgaagt tttgatctat aacggtatta
      241 acaattatat tcttcacctg aataattcat agctttaatt ctttggcaca ttttattgaa
      301 atatgtatca ctaatatccc tatcaactga ttttgactaa aaagttactt caaacattca
      361 ctgttcaaaa atattgagga ataaattttg ggtgtaaggc attttgagca tacttctccc
      421 tggataactt cccagtaaaa aaatcgttag attgttggat tagatccatt gactggctcg
      481 cctcaggcga gcaaagaccc tgtagaattc attctgatac acatgataac tagataattt
      541 gagatagact cgtctacccg tttggactaa tttcccagca actttcagaa acttcaatcg
      601 aatagaatga atggtcattc ccttttggac ttcttcaaag ccaatcgttc ttaagaagtt
      661 gactaagttg taagctatca agctgagcat cattcggaca tgattctcca aaaagcgagg
      721 actgtctgtt ttgtcaaagt aaaagccagc tttcgcttct ttaatgaagt tctccattgt
      781 gccacgtttg gcatagatag aaaagatggt atcaggagaa acattttctg atagattcgt
      841 cacgataaat tcatgtcgaa agagtagttc gcccgcttca cgtgttgagc gtatacacac
      901 gcgacgactt tgtgaccagg attgtgcttg ataagtggtc gagaagtact gaacttctcg
      961 ttcttcccac ttttgattat cgccataaag tactgatttc tcagctattt gacctagtct
     1021 acgattattc ttcagtcgaa taacataatg actctttttt gattcacacg aatcatacac
     1081 accaggtgta gcgaacccgc tgtctccacg aaccaagatg tcagtgtttg gtagagagtg
     1141 attatagtgc tctaataaag gtgtgagaaa ctcctttacg ccttttgatg tatattgatt
     1201 tcctgaacgt agttcagctt ttaagaagtc accagtcaat ccgtcaaaag ctaccaatgg
     1261 atggtaacca taggtttggt agtgtgtatt ataattcgtt tgttcttggt gaccaaatgt
     1321 atctgaatgg gtcgaatcta aatcaatgat taattccgta tcattacgga tgaggcgtgc
     1381 tttatcgata agttcttggt tcaaagcttg aagttcatgg atattctcct cagatagtcg
     1441 atctaaaaat cgggaaagtg aagattgaga agcgagttct ttcctatcta aaacagcttt
     1501 aaacacagga tcttgtctca ggagattagc tgcagaatca gctgagtaac cagcaattaa
     1561 ttgcataatg aactgctcaa gtatggataa gttgtcatga gtaaaatacg ctcgttcgtc
     1621 ttcaatgtga atgtgttgct tagccagttc agaaaaacca aagatgttca tcagctcttt
     1681 cactaggacg agacccgaat cactcgataa ttgaccacct gtatgagaaa tagtgatatt
     1741 tgaattgaat tttacttggt ttttgtgtaa gctagtcatg aaaagaactc ctttcttttg
     1801 gttggtttag tcactttaac catagcagaa aggggttctt ttttcatcac ttaacgggtg
     1861 aagatgaaaa agtaattgta agctgccgtg aggcagtttc acatggtttt aattaaaagt
     1921 atgaattatt cag
//
LOCUS       DAHUVV010000190         1932 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.195, whole genome shotgun sequence.
ACCESSION   DAHUVV010000190 DAHUVV010000000
VERSION     DAHUVV010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.195"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..596
                     /locus_tag="NK767_002877"
     CDS             <1..596
                     /locus_tag="NK767_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCH6695801.1"
                     /translation="KQFIHDLFLDAHFSRADIACDILGVPDDFITQYRIVDPVSFKPI
                     YGRSGKLETAYWGSRASERQVRMYNKKLEQERKKQIVPKEIETWWRIEMQLRRGKATD
                     WHAMVRESLDSFASPHYLPGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEE
                     SQNDELTNHLRETFKESANDLKIELDTWLLGLDVTEK"
     gene            796..999
                     /locus_tag="NK767_002878"
     CDS             796..999
                     /locus_tag="NK767_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695802.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            1559..>1932
                     /locus_tag="NK767_002879"
     CDS             1559..>1932
                     /locus_tag="NK767_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCH6695803.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLSVDDKVHPLKLSWSIDRITIVG
                     KLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQLFDQYGEDIAYIEMLKF
                     HPDKGRIDFNPNKIGEFLKGSM"
BASE COUNT          627 a          293 c          455 g          557 t
ORIGIN      
        1 tgaaacaatt cattcatgat ttgtttctgg atgcgcattt ctcacgtgca gatattgctt
       61 gcgatattct cggtgttcct gatgatttta ttacccagta tcgaatcgtt gaccctgtgt
      121 cttttaagcc aatttatggc cgttctggca aattagaaac agcgtactgg ggtagtcgtg
      181 ccagcgagcg acaagtgcgg atgtacaaca aaaagcttga acaggaacga aaaaaacaga
      241 ttgtaccaaa agaaatcgaa acatggtggc gtatagaaat gcagctaaga cgtggcaaag
      301 caacagattg gcatgcgatg gttcgggaaa gtttagatag ctttgcaagt cctcattact
      361 tgcctggaga tgtaaaacct gttgatagaa ttatgataaa agggctgaat caagatcatt
      421 cagagtgggc gtatattagc agaaatttga aatatagatt gcgcaaattg ttgaaagaag
      481 aaagccaaaa cgatgagttg acgaatcatt tacgagagac gtttaaggaa tctgcaaacg
      541 acttgaaaat agaattagac acgtggcttt tgggtttgga cgtgacagag aagtgatacc
      601 gaataagaaa agtcagatga gtgagagtaa atttacgtca atgtttaatg acgtgagttt
      661 acgataagcg attattttgc gtgaagcaaa ataaagcgta aaaggagttg agagcgtgac
      721 acgctttcgg ctcctttttt cgttgtcact tgtgacagcg aataccctcg gagagcattt
      781 ggaattgtga cagccatgtc acaattagga aaatgttact ctttgtcaaa gagtaacaga
      841 aaggtttaca ctctttttat tagcgacagc gcgaaggagg ggttcacctt agggtttagt
      901 atttcgttca aaaagggaac aaagaataca agcatttctc ataataatcg tgagttaact
      961 gatagccaaa aaatcaatgg tttgtctggt agaaaataat tagcagattt acaagtggga
     1021 aataaaaagc tgaaagaaga aaattgcatt tcaaactaca ttttctacaa tagctctaaa
     1081 atggcatatt ttgcgttctg agcggtttta gcgtttattt cgtttagtta tcggcacaat
     1141 cggtgaaaca cgttagagga gcgaaaatgg agcctgtaag gcgactggta gcggaacggt
     1201 cgtgttagat tgtgtagccg atagctgaac gaaataaatg ctggcagcgc cattctattt
     1261 cggcaggagg aggctcaagg ggttcgggga atgaaattcc ctgaggtctt tttatttttt
     1321 ctagaggagt gctataatca aagaaaatga gaaaaaagag taaaaaaata gtcgagcagt
     1381 gttgacgcac tctcgacctt tacggaaact acttcaccta gttccgtaac tcaagttcat
     1441 tatacgaata tttttaggtt tcttcaagct agagattttt ggatgtggat ttttagagtt
     1501 acgtgaactc tggaaattct ttccaagaat ctcttttttc gttgaggagg agctattttt
     1561 gagtaaaaaa ggcataatcg taaatgagtg ggaaaaactt aaccgtattt ctaacaggcg
     1621 gttaagtgtt gacgataagg tacaccccct aaaattaagt tggtcaatcg atcgaattac
     1681 aatcgtcgga aaactgaatg ataccatagc ttattggacg caaaatggag ttgtgtttgt
     1741 cgattttgag caattaatgc gtttgaatga aaatcaaggg tatttgaagt cggttggtac
     1801 aaatggttgg caattatttg accaatatgg agaagatatc gcctatatcg aaatgttaaa
     1861 atttcaccct gacaaaggtc gtattgattt taatccaaat aaaattggcg agtttttaaa
     1921 ggggtcgatg aa
//
LOCUS       DAHUVV010000191         1924 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.196, whole genome shotgun sequence.
ACCESSION   DAHUVV010000191 DAHUVV010000000
VERSION     DAHUVV010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.196"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..245)
                     /locus_tag="NK767_002880"
     CDS             complement(<1..245)
                     /locus_tag="NK767_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCH6695804.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQ"
     gene            483..1652
                     /locus_tag="NK767_002881"
     CDS             483..1652
                     /locus_tag="NK767_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCH6695805.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          682 a          323 c          298 g          621 t
ORIGIN      
        1 tcttgccata cctcctgaat ttgaccatca ggagtcgtaa tgaaaatagt atcattttca
       61 tctactaatt ctcttaagtt taaatattgt ttactagatg taaaattcga agctgctaat
      121 gacgagtctt cgcttttact ataaaatcct gttacttgta ttttatgttc ctggaaatac
      181 ttcccgaatg aacaacctac cttgccggct ccaataaaac caacgttcat aattatcacc
      241 tccataaaga agtgagacta caagtcccat tcttacgata tgagcttaaa aataaaaatt
      301 aaatcaaata tacagtatgt ttttttcaaa tatcatccat agttaattta tcataatatc
      361 ccatatttta aaataaaaga tagcgtaaaa tattttgtgt aaatagaaat ttagggtaga
      421 aaaaaaagtc cattctgtaa aattaaagtt accacacaga aattttatag gaggactttc
      481 tcatgactaa atttactata gaaattatgc aaacactact caataaaggg gatttagatg
      541 aattatttca ggatcattta gagcgagcga ttaattcact tctacaagca gagctaactg
      601 cttttttaga ttacgaaaaa tatgatcgaa atggattcaa ttcaggaaat tcccgtaacg
      661 ggaattattc acgtacattt aaaacagaat atggcgagtt gaatttaact attcctagag
      721 atcggaatgg cgaattttct caacaaacac taccagcata taagcgaacc aatgattcat
      781 tagaaacgac cattatccaa ttatttcaaa aaggcattac tatggctgaa atctccaaat
      841 taattgaaaa aatgtatggt caccactata cgccacaaac aatctccaac atgagtaaat
      901 tggtggctga agatgtttta gcttttaaag aaagaacgtt agaagccaat tattccgtta
      961 tatttatgga tgcgacgcat atccctgtga agcgacagac tgtttcgaag gaagcagtgt
     1021 atattacgat tggtattcgt ttggatggaa ccaaagaagt tcttgggttt actattgccc
     1081 caactgagtc tgcttatatt tggaaagaag ttcttcaaga tcttagaaaa cgtgggttag
     1141 aagaagtttt attagtagtg acagacggat taagctgtat tgaagaaagt atccatagtg
     1201 tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt atctagaaat atcgctcata
     1261 aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt caaattggtt taccaagcga
     1321 attcaaaaga agaggcattg gatcacatcg actttatgac taggaaatgg aaaaagcagt
     1381 atccaagagt cgtcaattta ctcttgaatc ctgccctatt aaccttttat aatttccctc
     1441 acgccatcag acgaacaatt tattcgacga acctgattga aggctttaat aagcagctaa
     1501 aacgatatac tcgaagaaaa gaacaattcc ctaatgaaga atctctagag agattcttca
     1561 tttctcaatt taatcaatat aaccaaaaat ttttaggtag aattcacaag ggatttaaag
     1621 aaattcagga tacattagag tcgatgattt aactgtaatt aacagaatgg attttccatt
     1681 tacatataat tcttgacgct accataatct aaataaccat catattcatc tactacttat
     1741 ataattgagt tttattaaac tttttaagtt tctattctat caaatattta aacaatctct
     1801 gcatccgaaa agactacctc gtatagtttt actaacaata tttttattag cgtgttcaaa
     1861 tcctgatttc ttgtatcaat tattttctgt tcactttcaa taagaaaaaa gtctttactt
     1921 ccat
//
LOCUS       DAHUVV010000192         1871 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.197, whole genome shotgun sequence.
ACCESSION   DAHUVV010000192 DAHUVV010000000
VERSION     DAHUVV010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.197"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(413..910)
                     /locus_tag="NK767_002882"
     CDS             complement(413..910)
                     /locus_tag="NK767_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCH6695806.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(950..1759)
                     /locus_tag="NK767_002883"
     CDS             complement(950..1759)
                     /locus_tag="NK767_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCH6695807.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT          539 a          348 c          328 g          656 t
ORIGIN      
        1 gtcgtaggtt cgagtcctac aaggtacata agaggttgtg aaaaagctgc cctgcatcaa
       61 gtaataagaa cagccaagta aaaacagctt cttatgtttt tacttggctg ttcttattac
      121 tgcagatgca agcttttgaa taccgtttaa ggaagaggtt gtgaaatcag cgttttgacc
      181 tgagtattag cttaacaaac ggaaaaatag ttcctcatat tttgccgttt gttaagctaa
      241 taccgagggt cagctgattg aacaccattt aagaaagagg tcgtgagcct gccgtttcac
      301 tctgtgtcat aaaaaagcca atctactttg atagaaaaag cacgtaggac taaaacagtt
      361 ctgcgtgctt tttttattgt gtgttaaata tcgtaaatga agatagatat ttttatgatt
      421 cgacgataga gacgttgaaa tctttcaaca gctctaaagc ggattctccg tgttttctac
      481 tggagactaa cattggatca acaaaaaagt ctgcttcagg aaaagcactc tgcaggccta
      541 tcagattaga gagtacacac aagtttgttt caatcccgac tacttcgatc tctttttgtt
      601 cttctttgct ttcaaataaa gtttcctgga tttcagctaa agtttcaggt ggaatcgtaa
      661 agtaatactt tttaatcact cgttcatttt cttgtggaga aagaagaggg atcaattgca
      721 aatcttctcg ttcttcttct ttgtccttca gttcaactgg tatatcccgt gtgaagagaa
      781 tatattcatt attttggcgt gctttagcta atctttgagc aatacgttct gccaattgat
      841 ctgactctgg aatataaaat tcgctgtttg gatcaagaat atgattttgc atatcaataa
      901 taactaacat tttctttcct cctacattca tatcgtcatt ttattctttt taatagaagt
      961 tttttaaaat ataagcaaca ccagcttctt cattcgtcag tgttacttgg tttgcaattt
     1021 cttttacttg tcgctgtgca ttccccatcg caatactcaa cccgctgtaa ttcaacatag
     1081 caatgtcgtt ttcttgatcg ccaaaagcca tgatgtcact cgattcgatt ccaaggtaat
     1141 ccgccagaaa aacaagtgaa tgagctttag tgatacctgt aggcgtgatt tctaagaaat
     1201 gcggatcaga gaatactgat tggataccat ctgcttgcag atctctttgt agcttctgta
     1261 gtttttcttc actcttatct gttaatgcta atttaagata gctaagagaa ggatcttcca
     1321 acatatttaa aatttgatca aaagaatggt tctgtaaatt gaaatgttca taataagggt
     1381 catctttgac tagatttgat tcatcgaatg tcagctgatc atcttgggtt tctcggctag
     1441 caatgatgga tattttcgct tcttgagcat agaagaaggc ttttctcagt gtatcgctat
     1501 ctatttttga acgtctgaga actttcgcca tttttccatc cttcatttca gcaatcagtg
     1561 aaccattgga agaaatcaaa tagccaccga ttcctaattc ggaaaaaata tctttagcag
     1621 aaatgctgtt ccttccggta gcgacggtta cgattccgcc attttcatca aatttccgaa
     1681 gtgcttggat attttcttct ggtatctttc catttcgatc aagcaaggta ccgtctaaat
     1741 caatcgctgc tagtttcatt ttatcttcct actttctttt tacatatcta ttttcctgtt
     1801 accttaagat taaatgagaa tttaaaaaaa tgaaactaac ttgctttgtc attatgaatt
     1861 ttttcaaaaa a
//
LOCUS       DAHUVV010000193         1854 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.198, whole genome shotgun sequence.
ACCESSION   DAHUVV010000193 DAHUVV010000000
VERSION     DAHUVV010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.198"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            3..674
                     /locus_tag="NK767_002884"
     CDS             3..674
                     /locus_tag="NK767_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCH6695808.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(1399..1734)
                     /locus_tag="NK767_002885"
                     /pseudo
     CDS             complement(1399..1734)
                     /locus_tag="NK767_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          682 a          300 c          235 g          637 t
ORIGIN      
        1 gtatgacaat taaaactagt ctccaaaatc aagcttacca atctattcgc cgacaaatta
       61 tctatgctga cttagaacct ggaaaaaaag tatcagaaaa aaaattagaa gaaacacttt
      121 ctattggtcg tacaccaata cgtgaagcac tgattcaact ccgacaacaa ggtcttgttg
      181 atacaattcc tcaaagcgga aattatatct cattaattga tttagattcg gccaaaaatg
      241 ctcgatttgt cagagaacat ttggaacgac aaattatgtt agagtgttgt gccaaaatga
      301 ataaccaaaa aaaacggatt ttagaaacta taatttcaga acaagaaaaa gctgttagtc
      361 aaaaaaatgc tcgtagtttt tttcataatg ataatttatt ccataaagct tgttttgaaa
      421 ttgcagagcg agaagaagta tggcaatggt tagaaaataa caatacacac ttagaacgat
      481 ttcgatggct acgagtaaaa acggaaggat taaagtggga aaaaattatg gaacaacatt
      541 atcaactatt tgaggcatta acaaaacaag atccagaaga agctaacttt ctaacatcgt
      601 tgcatcttca tttaatgctt aatgagcaaa atattgtttt agctactcat ccaaaatatt
      661 tcaaaaatat ataactttta actcttttta aattaaatgg ttgttctgat cagaaaaata
      721 tcgcttgttt tcttattatg taatatattt cataaactat tctgcataac aactattgtt
      781 tgtacttttt acattggata actaattatg actgctgata acttttgata ttaaactagt
      841 atctccaata caaataaaaa atagatatct tgttcatcaa aattagttcg tggatactta
      901 ccttcattta catcattcag cggtttccca ttttctttta ttctattatt tcattttcta
      961 atagacttgg cttcatttcg caatactttt tttcaatagg catcaaattg gtgttgttta
     1021 gatatttagt tgtagttttg tgacatgata agtatgctcc tttttctctt ttccactaat
     1081 acaacgatca aagggtgctt ttttcaaaaa actagaaaaa tcaaaataag attcgtattt
     1141 tgattctata tatcctgttt caactacatc ttatatttca caatagaata aatagccttt
     1201 tgcatagctg cttaacttat aagaaattgc aatttacttc ttcaaagtga aaacgaatat
     1261 gagtaaaata aaattattct tcttacagat aattaaaaat agcgcgtaac aaaaatcggc
     1321 attcaatcac ataattagga ttgatgccga ttacttatat tctacgtata gttttagtat
     1381 tcttattttt tatcaccagt aaccaattga gaacagccct aaagcagaat agtcttttat
     1441 ctcaaaacgc ttaatttcag gcttaggaat atgaataatc ttttgagtat attctttctc
     1501 ttttaatctt actgaagaaa taccactgcc tatctttttt acaaatattt catttggtgt
     1561 acagactaat aaaacaatat ttgtaatcaa tccttgcact aaagaattaa aaggaagact
     1621 atttaaaaca gggtgtgcat aactataaca tgtgaagtag ttattttgaa ctttaacttt
     1681 taaaaacttt aaaaataatc ttatttgttc ttctaaacaa tcatctatac tcatataatc
     1741 actccactaa ttaatctccc caaaaagaca cagcattaag agatatacca acacaacagt
     1801 gcaaataact ctacatcttc cttcgcaacc ttatttcaat aaaccataaa aatt
//
LOCUS       DAHUVV010000194         1778 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.199, whole genome shotgun sequence.
ACCESSION   DAHUVV010000194 DAHUVV010000000
VERSION     DAHUVV010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.199"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            78..1775
                     /locus_tag="NK767_002886"
     CDS             78..1775
                     /locus_tag="NK767_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695809.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT          575 a          334 c          301 g          568 t
ORIGIN      
        1 ttatattatc attgttattt ttaacaggaa ttgcttattt cttttatttt attttttctc
       61 ctgcggacag caatcaaatg aaagaaataa aagattcgtg ggttttctat acaaaagatg
      121 atcctgattt taagtttgac agcaagtaca ctcgtacgat tcctaccgtg gataaagacg
      181 aaacattcat tatggaaacg acacttgaaa aaccgttgga cgaagcaaat ttactaatca
      241 aaggaaacca tcaatggatt actgtatatc taggcgaaaa gattttatac caacggaaag
      301 attatcagga agaaagcaat cccggactct cattggctgt gatagattta ccagcaaatt
      361 atatcggaaa aaaattgacc cttacagtct catctcctta tcagaactat gctggactgc
      421 caccgaaagt ttatctagga acaactggac cattgattag ttttatttat tcacaatcag
      481 tgccgcaagt attgacgatg ataatcgctg tttttatcgc tatagggacc tttttctata
      541 cgatctactt gatgtataaa caaaaacgtc ttgaatactc acttattctc ttaagttgct
      601 ttgctttggc attaggattt gaagctgtat cagaagatat tttaagtggt attctttttg
      661 aaccattcgt tcattctttt ttatctcatt tatttattat cttaacgtca gctttcatca
      721 tctgctacta ttggagccga atgttttact ataaaaaatg gtacggaatt ttttgtacgt
      781 tccaactttt gatttttatc ggcgttctgc tttatgcaac ttttactcct gcagaacttc
      841 ctgaattaat gccgattgct aatattgcta gtgttataag cactttagtc acttcacttg
      901 catgcattgg cgaagcatat aagaataacc gtttttatgt agcttgtaca ccttggatcg
      961 tcttggtagc cattatccat tgcttgattt acatccaaac ggcattaggt atctatcaaa
     1021 caacgatcaa ttggtctacg attttatata ttatcattct aatcgttatt atcagctaca
     1081 atctaatcga tcacatctta aataccgaca aagatcggag acaagtagat ttcttaaaaa
     1141 tcaaaaatga tttgttggag caacattatg agcagcttcg acagcatatc caagaagtaa
     1201 atacacttcg cctggaattt gtccaagaga tggagaatct gcaagattta atgcatacgg
     1261 accaaacgaa agctaaaaac tatgtggaaa agatattaga agaagcaaaa aatttcgaaa
     1321 tgctgtgttc tttttgcaac caccaggtga caaatcttat cttagcgcgt taccagaagt
     1381 tagctgccaa acggaatatt cagataacct tccaagcaga tcttcccgaa gaacttccta
     1441 tcaacgatga cgatctggct caacttttga tccacgcttt agagcattcc tttagagaaa
     1501 ctcatgccat cgaaaatcca ctgtatcgaa aaatttattt gtcgatacac gaacaagagg
     1561 aacatttcgt catttgttgc gaacatagtg cacattacga tacaaaccta tttagtcgag
     1621 gcatcacaga agaattagat gatcaggaac gttttgactt aatgatgatg aaagacgtag
     1681 cggatcgtta cacaggaaca ctaacgcagg aaaaagatac tttcattgat cggatcactg
     1741 ttcaactctc tcgaaattac ccgaatcctg tataaaaa
//
LOCUS       DAHUVV010000195         1770 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.200, whole genome shotgun sequence.
ACCESSION   DAHUVV010000195 DAHUVV010000000
VERSION     DAHUVV010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.200"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..153
                     /locus_tag="NK767_002887"
     CDS             <1..153
                     /locus_tag="NK767_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCH6695810.1"
                     /translation="VGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWY
                     LNIPKR"
     gene            268..1476
                     /locus_tag="NK767_002888"
     CDS             268..1476
                     /locus_tag="NK767_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695811.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            1460..1630
                     /locus_tag="NK767_002889"
     CDS             1460..1630
                     /locus_tag="NK767_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695812.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT          675 a          228 c          300 g          567 t
ORIGIN      
        1 gttggctatc atgtatcttt taacatgcgc aatttcccta ataagaaagt atacgtcatg
       61 atgcgggcaa cgaatgatcc agaaggaaac actaaaggcg gtgcgcaaga tttccatgac
      121 aaacgttggt atttaaatat tcctaaacga taaaaatagc tcctcgttga ggagcagtac
      181 atagaattga aaattaacgt taaatcaaaa aaatatttac aagatgttta tttaagtaga
      241 taataaaaga aaaaagcagg tgaaattgtg acagcagaaa ttggtattat gaataaaagt
      301 ggaatcgtgt tagcttcaga ttcagcatct acgattggag atagcaaagt atataatact
      361 gcaaaaaaat tatttacttt ggattccatg cattctgtag gtatcatgat atatggtaat
      421 gctgaattta atggtattcc ttgggaaata atcattactc aatataagaa aagcattggt
      481 agttctgttt ttaatacttt ggaagaatac gcggataatt ttatagaatt tgtcaaaaca
      541 gcatctttta ttagaagtga acagaccgag caagaacaaa tgattggggt ttttcaaaaa
      601 ataatttcag gtttatttga gagtatagaa gtagatatta actttttaat aagccaaggt
      661 acacaaatag acaaagatgt cctagtgaag ttattacaaa caaaaatgaa taccaatttg
      721 tctcaacagt cccagacatt cattttagat attgaaaagg cgttatttct tagtaactat
      781 ggagaaatat taaaagatat tcttaatagt atttctacaa tggaaggtgt ttcagaagct
      841 atatcagagg aaattcaaag ttacgtttat gaaattataa ttagagacga tgtttattct
      901 tcccctacag gaatagttat agcaggatat ggaagaatgg atattttccc taaactatat
      961 tcttataata tgtttggatt tgttatgaat attctgaaat actcagaata tgaaagtgct
     1021 caaataggaa atgacaatgg ttcattaaga tctactatat taccatttgc tcagtctgat
     1081 gtggtaaata ctgtagttca aggggtagat ccacaaatta ctaattatct gtcgagtcaa
     1141 gtcgatagtt ttgacgataa cggaaaaaat acctatatta atattataaa gaatatttca
     1201 gagtttcaac aaaatcaatt catatttccg ttactaaaca tgattgcttt actcccagtt
     1261 gaagaaacag caattatcgc agagacttta ttaaatttaa ccagttttaa acgaaaatat
     1321 actacttctg tagaaacagt aggtggccca attgatgttt tagctattac acctaatgat
     1381 ggccctattt ggattaaaag aaaacattat tttgatattg ataataatat tggatacaga
     1441 ttgcgaaagg agagagcgaa tgattacaat aattaaacct caggaaaaat cccctgtttt
     1501 gaaatttaat gagagcaatc tagcccataa taaaatgagt ataactgata cagtatttga
     1561 tgtaatggtt gagcaacaaa aaaaaattaa taataacaat agacaaagaa aagaaattaa
     1621 ccaatattaa tgttttttct gttgcgtttt ttgcagtcat aatttaaaac ctcgctcaat
     1681 cttgggcgag gtcttttttt atttttctga aaagttaaag taaataatag cataaaataa
     1741 tatattttac aaagaataag tacaatctag
//
LOCUS       DAHUVV010000196         1727 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.201, whole genome shotgun sequence.
ACCESSION   DAHUVV010000196 DAHUVV010000000
VERSION     DAHUVV010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.201"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..582
                     /locus_tag="NK767_002890"
     CDS             <1..582
                     /locus_tag="NK767_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695813.1"
                     /translation="PELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIF
                     FDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEV
                     LLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNI
                     VLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            569..>1727
                     /locus_tag="NK767_002891"
     CDS             569..>1727
                     /locus_tag="NK767_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCH6695814.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGII"
BASE COUNT          573 a          221 c          277 g          656 t
ORIGIN      
        1 ccggaattgt tactttatga tgtaaaaaat ataaaaaaaa ttcccaaaat agatgaaaac
       61 agtaaattga tgaaaaacat acaatcaatc tttgggatag aagaagtttt tgtcggagat
      121 aaatgtatct tttttgatca acctttatta tctgatggtt atggcataga tgaaacagat
      181 attttcgcta aagtaaatag tatattttct ggaaaaaaag aaactttaat taagcttcat
      241 cctagagcaa ggacaaatcg ttatggtgaa aaaaacatat tgaattccag tttaccgttt
      301 gaagtcttgc tacttgaaaa tgacttctct tcaacacttt tgattagtcc tatatctaca
      361 atttcttttt caccatctat gatgtttgaa aagacgatca actctattat gttggctaaa
      421 ttaattttaa atgaatatac agcattatct gaagataaaa aagaaacatt gacaaatatt
      481 gttcttttct gtgaacatta taaccgtcta tcagaagaaa aaattttact cccagaaaac
      541 tgggcagaat taagaaagtt ggtagagtat gggattattt aacacacttt ccaagtcaaa
      601 attagttaag tccagtttct ggtatactat agggaatttt tttattaagg gaatttcatt
      661 cataactatt cctatttttg taaatattac tactcaagca gaatatggtt tgattaataa
      721 tttcacttca tttgtatcaa ttttttcgat tcttgttggg ctaagcctaa attcggcaat
      781 taataatgca agttttgact ataaagatga cataaatgaa tttatgtcat cggtactttc
      841 tctagctaca ctttcgttaa ttgtgttctt attaataggg aacatatatt ttttgttttc
      901 aaatgctttt tttgatatga accaacttgt atttaatttg atgttattac aaagttttgc
      961 tagttttgta attagttata tccagtctta ttttacgatt aactttaacc attttaaata
     1021 tttgatttta tcttttatat ctactttttt gaatattgca atctctgtag ctttgatgca
     1081 gacattatat tttgatgata aatatgttgg cagagtagtc ggcggaacac tgtcatttgt
     1141 gttgataggt ttagtaattt atttaagaat aatgataaag ggtgctcatt tttttgataa
     1201 aaaacagtgg agatacgctt taaaaatctc tttgccgcta atcccacatt ccttagcaaa
     1261 tatagtttta gcacaattcg atagaattat gattaattct tatttaggtc ctgctgcggg
     1321 gggaatatat tcctacatta gtaacatagg tattattcta agcgtaatat ggatgtctac
     1381 taataacgcc tgggttccat ggttctatgg tgagatgagt aaaaataatg aaaaaaaagt
     1441 gaagaaagtt agtaactatt atttagtagt ctttactttg attacgatga ttgtaatggt
     1501 agtttcgatt gatttagcaa aatttatggc tccgaaagac tattattctg ggttatcttt
     1561 agtggttccg ataaccttag gctatttctt tcagttttta tatagtttac cagttaatgc
     1621 tgaattttat gagaaaaaaa catcgtttat tgcgattggt acagtagcaa gtgctatagt
     1681 taatatacta ttaaacatga ttttcatacc taggttagga ataattg
//
LOCUS       DAHUVV010000197         1655 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.202, whole genome shotgun sequence.
ACCESSION   DAHUVV010000197 DAHUVV010000000
VERSION     DAHUVV010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.202"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(188..1207)
                     /locus_tag="NK767_002892"
     CDS             complement(188..1207)
                     /locus_tag="NK767_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCH6695815.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1218..1424)
                     /locus_tag="NK767_002893"
     CDS             complement(1218..1424)
                     /locus_tag="NK767_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCH6695816.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            complement(1557..>1655)
                     /locus_tag="NK767_002894"
     CDS             complement(1557..>1655)
                     /locus_tag="NK767_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCH6695817.1"
                     /translation="KRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT          469 a          338 c          279 g          569 t
ORIGIN      
        1 ggttgttgcc acataaagcc ataaacaatc aaccagtaga atgtgtggca acaaacctgt
       61 tatcgcatag cttggagtgt gactattatg ggtgatagtg aagatatgcg ataacatcac
      121 tattttatcg aatgattttt atagttgtca atatagttat gtaatgctcc tcaacgagga
      181 gctattttta tcgtttagga atatttaaat accaacgttt gtcatggaaa tcttgcgcac
      241 cgcctttagt gtttccctct ggatcattcg ttgcccgcat catgacatag actttcttat
      301 tagggaagtt acgcatgtta aaagatacat gatagccaac atttccagaa gtattataag
      361 cttgatttac atctgatcta taaattccat cagctcttac tcgagctaat tctttcccag
      421 tattgtaatc cataatgaag atatactcgt atttatagtt agcaatgtgc catccagcta
      481 catgcaaatt tgcattttcg atttctccaa actgatcaat gtgggcgtaa ttcgttccat
      541 ctgtcagtgt aggattagct gcaccagctc gtgttggatc aatgacaggc ttgttttcag
      601 aagttgttgg attttcatcg gtaaatccat gagctaaatc atatgctaat ttttctttac
      661 ttacgcccat ttcagaaaga taaccgtaag gatctgtatg atcgccccag atgttttgtg
      721 ttacccataa atgcgatttg attcccggtt ggttataagg agtgtccaac gttaatggaa
      781 taccatattt cattgctgaa tctctagcca attcaacgta tgccttgtag tttttctcaa
      841 acgttgcttt atcatgtgtg tgttgtaact caatctgcac aggactgttg gcattagcat
      901 acgaaccagc accgtactgt acataaccag gttgaccgac ttgataaaca attccgccgt
      961 ctcccacaat ataagcagta taagcgctag tccatgaacg ttgcatatac tgcgcttcat
     1021 tgcgtcctgt tgctgtttca ttagccgttt catgcagtaa aatatactga ttatttgcta
     1081 cttgtgagct accttcattt gcgcccaaat taaattcatt gttgatagta taggcaaatc
     1141 cgttaattgg caataaaaaa agagccgtta acaggctcat cgcagtaata gtaattttct
     1201 ttttcatatc tatcctccta tttatctgat gaaattttct tatacgttcg atttgataca
     1261 cctaaaatac tccctagaaa tgctccaaaa cctgtaataa tcatcaccgt tatatctgtg
     1321 taccgccagt taacaccttt tccgacaaga ccgacaaaag tcgctaaagc ggggataact
     1381 actatagcga tccatttcaa aatatcaaag gttctattgt tcatactatc cacctccctt
     1441 cattggttgg gctactttct ttttcaatcg cttgttttct tctatcaata tttctctttc
     1501 tgaaagcaag gcatcaattt gcccgtccag ttgttcaatt ttatctttct taattgtcaa
     1561 attaagttag acagatcatc aatacttacg taactcaaaa atacttccaa aggtgttcga
     1621 taatccagtg attttctagg aatattattt cgttt
//
LOCUS       DAHUVV010000198         1653 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.203, whole genome shotgun sequence.
ACCESSION   DAHUVV010000198 DAHUVV010000000
VERSION     DAHUVV010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.203"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(68..1546)
                     /locus_tag="NK767_002895"
     CDS             complement(68..1546)
                     /locus_tag="NK767_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6695818.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          528 a          301 c          227 g          597 t
ORIGIN      
        1 accttttctt tgaaaaaaac ataaatcagc taataaatat gtgagacaaa gctgccatcc
       61 ataaatacta gctgattttt tcagttaaat gtctattttt ttctctgatg atggtatttc
      121 caaaatattc ccagtcatct ggagtcggtg gatttttcca aattatctgt tctttattaa
      181 atttttcaag taaacaatca gataaataaa tattcgtacg agaagtgatt cgactctcaa
      241 tcacaatatt gtaattttta aatcctttca cttgagaaat aatgtaatcg gtataagcag
      301 atccttgcga aaaatcaata cagacatgaa tcggtgcttc aaaatatgat accggacaaa
      361 taccgaccaa taaaaagaga taatcataaa ataagcgaat catgaaacgt tcatcttttc
      421 ccaagaaata gcgtttattc tcttgtatga ccttttggat tgctgtactg aatattggat
      481 acgtctcgaa gaaaaactgt agctgctttt tagacgtaaa gctagtcatc tcaaagtcga
      541 atatccaatt ttttcgattg acatgcatca tacccatacg aagttctttt cgtatatata
      601 gtctttcttc ttcagagatt tcaatcgaaa atggtaattg atcaatcaat tccactgaca
      661 ttttttgact aatcatgtgg atatcatgaa aatgagcttc cgcaagtcca aatgcgccat
      721 ctaattcaaa ttcttcttcc gtgatcaaaa acaacatgat ataagaaatc tcatcaatga
      781 tctgctcttt agggatatga agaaaagaaa tatgtgataa atccttgatg atcttcattc
      841 cttctgagct cttaaagtat tcatctgaaa agaaacgttt tccgattggt tgattcccat
      901 aggccctcaa caaactaata gtggttagca aattgaattt gattcgtttt gttgcagaca
      961 gttttaaatt gaacagatat tcaaaatatt gcacgatact ctttgcttca ttcatgacgc
     1021 tttctctaaa tggcatagaa aaaccattaa agaccccgaa atatacccca aataatacgt
     1081 tccgtatgtt tatttcttcc ccaactaata tgtttttctt taactttatt tgatgctgtt
     1141 caagaaaaga gactaattct cttcttttta cataggctct tgatatgctt aaaaaatgat
     1201 tctctgcaaa tttttcgatc gttacttttg aatccacgat ctccgttaac agtcgagcaa
     1261 tttttgatcg ttgaaagtag attcttctta aatttttgat atccgtcaaa ttcagacgtt
     1321 cgacatggat ctctgcttct tctgaaattt ggatctctgg ctttgttttg aattcagtaa
     1381 attctttatt taattctatg atataatttc ttgttttaaa tttagacaaa cctaatgttt
     1441 catataactt aggcagcact acataattct ttttctcaaa ttctaaatat ctagctagca
     1501 gcagtttttc tctatcttcc tcttctataa ggtaatccaa cttcatatcc tacacctcaa
     1561 aaaatgcctc ataccaatta cttgatatgc agatgaaagt tcatcttttc acctatactg
     1621 attttatgat ggattatgct tgttttttca tca
//
LOCUS       DAHUVV010000199         1648 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.204, whole genome shotgun sequence.
ACCESSION   DAHUVV010000199 DAHUVV010000000
VERSION     DAHUVV010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.204"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            132..1598
                     /locus_tag="NK767_002896"
     CDS             132..1598
                     /locus_tag="NK767_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCH6695819.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          461 a          296 c          338 g          553 t
ORIGIN      
        1 tgatattgcc attaaaaatt acttgctttt atagaaaatt agtttatatt tcttaaataa
       61 gttttttagt taaatttttc ggtaaagatg tataatattt tctgaaaatt taaagaattg
      121 gggtatagat tatggaaaaa cagcaagaca agggcttctt cgggcagccg aagggattgt
      181 caactttgtt cttcactgag atgtgggaac gatttagtta ttacggtatg cgtgcattac
      241 ttatctatta tatgtacgat acagttgcca acgggggact tggattacca cgagcaacag
      301 cattagctat catgtcgatc tacggatctt tgatctacat gtctagtatc gtcggcggat
      361 gggtatctga ccgagtgtta ggagcaaaac gcactgtatt cttcggtgga gttttcatta
      421 tgcttggcca catcgtcttg gctttaccat ttggtgtaag tacattgttt gtttcgatgg
      481 cattgattat tatcggaacc gcctttttga aaccaaatgt ttctggcatg gtcggacatc
      541 tttattcaaa gactgacttg cgaagagatg caggattttc gattttttac atgggtgtca
      601 acttaggggc attattagca ccactaatcg ttggtacaat cggtcaagaa tataactatc
      661 atttagggtt ctctttagct gctattggga tgttctttgg attattacaa tacatcatcc
      721 aaggacgcaa gacattagac ggagtgggta caacaccgcc gaatcctttg acaactgaag
      781 aacgtaaagg attcatccgt aacttgatca tcgcacttgt tgttatcgct gcagtattcg
      841 gcggggcaca actaactggt catctgacaa ttgacttttt tgtcaataca atcagtgtat
      901 tggggattat cttaccgttg tattacttta tcaaaatgct gacttcaaaa gatgtaactg
      961 ctgaagaaaa accaagagta tgggcttata tcccattatt ccttgcagca atcattttct
     1021 ggtcattaca agaacaaggt tcatctatct tagctttatt tgctagtgaa cgtacacaat
     1081 ctacgctatt tggtgtaacg atccaagcaa gctggtatca atcattgaat ccaattttca
     1141 tctttattct gacaccagta ttcgtaacga tgtggacgaa acttggtgaa cgtcaaccat
     1201 ctacagtagt gaaattctct cttggacttt tatttgcagg attatcgtac ttgctattga
     1261 tgttgccggg tatgctttat ggtacaagtg gaaaagtaag tccattgtgg ttgttgggaa
     1321 gtttcttcat cgtggttatt gctgaattgt gtctttcacc agttggtttg tctgtgacaa
     1381 caaaattagc accacgagca tttgaatcac aaacaatcgc tatttggttc ttagctgatg
     1441 cttcttcaca agcaatcaat gcacaaattg cacgcttcta cacaccggga acagaatctg
     1501 cttacttcgg tattatcggt gcagtagcaa tcgtcggtgg tatcttgcta tttactgtga
     1561 aagaaccaat caaaaaacta atgggctcta ttcattaatc aatgaacata aaaacaggaa
     1621 ttgcttattt gcagcagttc ctgttttt
//
LOCUS       DAHUVV010000200         1612 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.205, whole genome shotgun sequence.
ACCESSION   DAHUVV010000200 DAHUVV010000000
VERSION     DAHUVV010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.205"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(62..280)
                     /locus_tag="NK767_002897"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="NK767_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="NK767_002898"
     CDS             complement(546..740)
                     /locus_tag="NK767_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695820.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="NK767_002899"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="NK767_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          570 a          242 c          214 g          586 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt ta
//
LOCUS       DAHUVV010000201         1561 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.206, whole genome shotgun sequence.
ACCESSION   DAHUVV010000201 DAHUVV010000000
VERSION     DAHUVV010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.206"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..451
                     /locus_tag="NK767_002900"
     CDS             <1..451
                     /locus_tag="NK767_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HCH6695821.1"
                     /translation="NLVTAARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSG
                     LVERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVF
                     KGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            696..>1561
                     /locus_tag="NK767_002901"
     CDS             696..>1561
                     /locus_tag="NK767_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCH6695822.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKLNL"
BASE COUNT          615 a          184 c          317 g          445 t
ORIGIN      
        1 aaacttagtg actgctgcga gagataacga taggttaaaa cagcatgtta gaaatctcat
       61 gagtactgat atggcgagag aatataaaaa attaagtaaa gaacatgggc aagttaaaga
      121 aaaatatagt ggtcttgtag agcgatttaa tgaaaatgta aatgattata atgagttgct
      181 tgaagaaaac aagtctttaa agtctaaaat aagcgattta aagcgtgatg tgagtttaat
      241 ctatgaaagc actaaggaat tccttaagga acgtacagac ggcttaaaag cctttaaaaa
      301 cgtttttaag gggtttgtag acaaggtaaa ggataaaaca gcacaattcc aagaaaaaca
      361 cgatttagaa cctaaaaaga acgaatttga actaactcat aaccgagagg taaaaaaaga
      421 acgaagtcga gatcagggaa tgagtttata aaataaaaaa agcacctgaa aaggtgtctt
      481 tttttgatgg ttttgaactt gttctttctt atcttgatac atatagaaat aacgtcattt
      541 ttattttagt tgctgaaagg tgcgttgaag tgttggtatg tatgtgtttt aaagtattga
      601 aaacccttaa aattggttgc acagaaaaac cccatctgtt aaagttataa gtgaccaaac
      661 aaataactaa atagatgggg gtttctttta atattatgtg tcctaatagt agcatttatt
      721 cagatgaaaa atcaagggtt ttagtggaca agacaaaaag tggaaaagtg agaccatgga
      781 gagaaaagaa aatcgctaat gttgattact ttgaacttct gcatattctt gaatttaaaa
      841 aggctgaaag agtaaaagat tgtgctgaaa tattagagta taaacaaaat cgtgaaacag
      901 gcgaaagaaa gttgtatcga gtgtggtttt gtaaatccag gctttgtcca atgtgcaact
      961 ggaggagagc aatgaaacat ggcattcagt cacaaaaggt tgttgctgaa gttattaaac
     1021 aaaagccaac agttcgttgg ttgtttctca cattaacagt taaaaatgtt tatgatggcg
     1081 aagaattaaa taagagtttg tcagatatgg ctcaaggatt tcgccgaatg atgcaatata
     1141 aaaaaattaa taaaaatctt gttggtttta tgcgtgcaac ggaagtgaca ataaataata
     1201 aagataattc ttataatcag cacatgcatg tattggtatg tgtggaacca acttatttta
     1261 agaatacaga aaactacgtg aatcaaaaac aatggattca attttggaaa aaggcaatga
     1321 aattagacta tgatccaaat gtaaaagttc aaatgattcg accgaaaaat aaatataaat
     1381 cggatataca atcggcaatt gacgaaactg caaaatatcc tgtaaaggat acggatttta
     1441 tgaccgatga tgaagaaaag aatttgaaac gtttgtctga tttggaggaa ggtttacacc
     1501 gtaaaaggtt aatctcctat ggtggtttgt taaaagaaat acataaaaaa ttaaaccttg
     1561 a
//
LOCUS       DAHUVV010000202         1554 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.207, whole genome shotgun sequence.
ACCESSION   DAHUVV010000202 DAHUVV010000000
VERSION     DAHUVV010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.207"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            192..446
                     /locus_tag="NK767_002902"
     CDS             192..446
                     /locus_tag="NK767_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCH6695823.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            447..764
                     /locus_tag="NK767_002903"
     CDS             447..764
                     /locus_tag="NK767_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCH6695824.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1323..>1554
                     /locus_tag="NK767_002904"
     CDS             1323..>1554
                     /locus_tag="NK767_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695825.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTD"
BASE COUNT          550 a          222 c          298 g          484 t
ORIGIN      
        1 ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat tttgatatcg tttgtatatg
       61 ttttcagcgt tctaagtggc ttattttgtc caataataat atggagtgga atcccttaag
      121 aaacaccgtt ggcaattgtt gttataacat gttataattt attcatcaaa aactaaaagg
      181 agtgtgataa catgggaaaa gaactaaagg ttagaaagat cggcaatagc gtgggagtga
      241 tccttccaag ctctcttgga ttaaaaagcg gagacattat ccaagccaaa caagaaggca
      301 acttattcat attagatact actcaaatag caaaagaaca cgatcgcaaa ttgattgaag
      361 aaagtttcca agactttgag aaaggtttaa cagtttcaga aattgaaatg gtaaaagctt
      421 tcggaaaata cggctggagt gaatagatga aagagtacac gctagtttat tctaaaagtt
      481 ttcagaccag ccttgaaacg ataatagaag aatgggaaaa tgagcttgct ctttctccag
      541 ccagcataaa aagatttgta ggtgcgatcc agaaggcttt agaactaacc aaaaattttc
      601 caactatgca tgaggaagtt tccgaaatct atggaatgga tatcccaaca tatcggatcg
      661 tgatcggcaa aagctatgcc ttattttatc ggatcgacca tgaaaaaaaa gagatacttg
      721 taggacgtat tttccaatct aaacaaatga aactaggctt ttaacaataa ttaggaaaaa
      781 gtcgctcatc ctctacaggt gggtgattaa tgccgctcga tatggttgtt aaccgtcggt
      841 tactcgaaga ttgatatata agacttagta cctttttatt ttgctccatg ttatggccga
      901 aggtctaaag atacaatcat tagtctagag aaaaattaag aaccgtagga actacgggga
      961 tcgcttggtg aataagagac actgctatta gtgaaaaaat aagatatcaa gtacgctcta
     1021 ttcccaaaaa tttcgtgact ttactcatga gaggttcaag tagtatattt ttaatcaaaa
     1081 ttgacgtaac ttttaataaa tactacctaa ataaggtagt atttattttt gtgttttaaa
     1141 agaggaagtc caatatatct atgggaaact tcttaaatat tatgttggta catgaaaata
     1201 tacatccctg ctagactatt tacaacattt tactttgtac gtattcttgt tttaaaagga
     1261 atgggataaa atataggtac aataactatt tgatgctaaa tataattaat ggaggagaga
     1321 tagtgaacat acttaatgaa aaaagatatg tatggtttgc tggtaaaaaa tatattacta
     1381 ctttcaaaag attatccgag ttaatgcagc agactgttaa aagtgctatg cctgaaggat
     1441 acaagtttaa ttttgttcaa tcttcagtat ctactgaaag tatctatttt tcaattcttt
     1501 ctgagagtag gctttttcat tttagaataa gttctcatgt aaagaaaact gatg
//
LOCUS       DAHUVV010000203         1549 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.208, whole genome shotgun sequence.
ACCESSION   DAHUVV010000203 DAHUVV010000000
VERSION     DAHUVV010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.208"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            235..1404
                     /locus_tag="NK767_002905"
     CDS             235..1404
                     /locus_tag="NK767_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCH6695826.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          569 a          221 c          250 g          509 t
ORIGIN      
        1 attactatgt ttgggtttta tagcaaagaa agtttaataa gagtttggac ctagcaaaca
       61 atattaatga ggtcattaaa tcttattatc tttagtgata taacgaaata tctacacaga
      121 caaaatgata gcgtaaaata ttttgtgtaa atagatattt agggtagaaa aaaagaaagt
      181 ccattctgta aaattaaagt taccacaacc gaaattttat aggaggactt tctcatgccc
      241 aattttacta cagaaattat ggaaacacta atcaataaag gtgatttaga tgaattattt
      301 cgatgtcatt tagaattagc tgttaattca ttattgcagg ctgaattaac agcttttctt
      361 gattatgaaa aatatgatcg tgctgggttc aattcaggta attcccgtaa tgggaattat
      421 tcacgttcat tcagaacaga gtatggagaa ttaaatttgg taattcctag agatagaaat
      481 ggtaagtttt ctcaacaaac tttacctgct tataaaagaa ccaatgactc tttagaaacc
      541 actattattc agctatttca aaaaggaatt actatgtctg aaatctctga attgattgaa
      601 aaaatgtacg ggcatcacta tacgccacaa accatttcaa atatgactaa aatagtttct
      661 gaagatatta ttgcctttaa agaaaggtcc ttagaatcca gatactctgt catttttatg
      721 gatgctactc acattccttt aaaaagacaa actgtttcaa aagaagcagt ttatattgta
      781 attggcattc gattagatgg aaccaaagag gttcttgggt ttagtcttgc tccaactgaa
      841 tctgcttatg tttggaaaga gattcttcaa gacctaaaag accgtggttt aaaagaggtt
      901 ttattagtcg taactgatgg tttaagtggc attaacgata gtattcatag tgtttatcca
      961 aatgctcaat ttcaacaatg ttgtgtccat atatctagaa atatcgctca taaggttcgt
     1021 gttagtgatc gacaagaaat ctgtagcgat ttcaaattag tttatcaagc aagttcaaaa
     1081 gaagaagcaa ataatcaaat aaggtttatg atagataaat ggaaaaagca gtatccacga
     1141 gtggttaaat tgctcatgaa tcctgctata ttaacctttt ataatttccc tccgtcaatc
     1201 agaagaacta tttattcaac taatttgatt gaaggtttta acaaacaatt aaagaaatat
     1261 accaaaagaa aagagcaatt tcctaatgaa gaatctctgg agagatttct agtatctcaa
     1321 ttcaacaact ataatcaaaa gtttctatgc cgagttcata aaggttttaa agaaatacat
     1381 gatacattag aatcaatgtt ttaacttgat caacagaatg ggcttttcca tttacacaaa
     1441 attattgacg ctcccgacaa aattatgatc ttgcgtaaag ttataaaata ttaataaaaa
     1501 tattttatta atgaaaagct tggtagcacg gttattcata tgctaattt
//
LOCUS       DAHUVV010000204         1540 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.209, whole genome shotgun sequence.
ACCESSION   DAHUVV010000204 DAHUVV010000000
VERSION     DAHUVV010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.209"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..1490
                     /locus_tag="NK767_002906"
     CDS             <1..1490
                     /locus_tag="NK767_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCH6695827.1"
                     /translation="QQQTIQNLTTEIQLLNEKVNYLTNKLFGRSKETLFEETNGQLNL
                     FSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLLPLEEQFCEHCGS
                     QMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSL
                     GSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKAVQNALDPLGER
                     LRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPK
                     TFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQ
                     LDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACK
                     LRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAE
                     LNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          520 a          280 c          318 g          422 t
ORIGIN      
        1 cacaacaaca aaccattcag aatcttacaa ctgaaatcca actattgaat gaaaaggtca
       61 attatttaac caacaaatta tttggacgct ctaaagagac tctctttgag gaaacgaatg
      121 ggcaattgaa tctttttagt gatgaagaaa tttctgtaag tgttccagaa gcagctgcca
      181 ccattattcc agtaaaagga catcaacgag ttgtaggaac gaaagcagac aaaatcaaac
      241 acttacctat aacagagaaa gaacatctcc ttcctttgga agaacaattt tgtgaacatt
      301 gcggttcaca gatgaaagat atcggacgga caaaagtacg agaagaaatt cgtttccatc
      361 aggctatgtt ggattgtctc acccattatc aacataccta ttgttgtaaa agctgcgaaa
      421 aagaaggact ttcttctttt aaaaaagcga tcgttcctaa acctttgatt tctaatagct
      481 taggttccaa tagtttagtg gcggaaacga tccggatgaa atttggtcag aaagttcctg
      541 cctatcggca agagaactat tggaaacaaa cacatgggtt ggatatttcg agagataata
      601 tcaccaattg gcatattaaa gcagtgcaaa acgcgttaga ccctttagga gaacgattaa
      661 gagtctatct taatcaagaa gagattctac acggggatga aacaagttat cgtgtgattg
      721 aaagtgccaa aacagatacc tattactggc agttttgtac aggaaaagac agtcagcatc
      781 ccatcgttta ttatcaccac gacgaaagtc gtgctggaga tgttccgaaa acttttctca
      841 aggagtttac agggtattta cactgtgacg gctatagcgg gtataatgcc gtggaaagtg
      901 tacgacttgt ctattgtttc gcgcatgttc ggcggaaatt tttcgaagca attccaaaag
      961 gaaagaaaaa cacggatatt cccgcagctc aagcagtcaa acaattggat aaatggtttg
     1021 tcctagaaaa gaaatggaaa gacttttctc ctgagaagcg attaagctgt cggcaacaag
     1081 aactgcgtcc attatttatt gctttttatg agtggatggc aacgatagat cctgttgcaa
     1141 aatccaagtt ggatgcagcc gttcaatatg cgtgtaaact acgaagtggt tttgaaccta
     1201 tttttgaaga tggtcgttta gaactcacaa ataatcgtgc ggagcgaaat atcaaagagc
     1261 tggtgatagg acggaaaaat tggttgcatt ccacaagtct tgaaggagca cgaacttcag
     1321 gaatcattct gagtgtatat aaaacagcag aacttaacgg attgaatcct gtaaagtatc
     1381 tagagtttct atttgataag ataccaaatc ttcccgtact ttcagcagaa actttagatc
     1441 aactgttgcc ttggaacaag gatgtacaac aacatttttc tcgtaactaa aaacgccaaa
     1501 cattcccgat tctattctat aggtcgggtt atttgacgta
//
LOCUS       DAHUVV010000205         1507 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.210, whole genome shotgun sequence.
ACCESSION   DAHUVV010000205 DAHUVV010000000
VERSION     DAHUVV010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.210"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..1412
                     /locus_tag="NK767_002907"
     CDS             <1..1412
                     /locus_tag="NK767_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCH6695828.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
BASE COUNT          595 a          233 c          330 g          349 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagat
//
LOCUS       DAHUVV010000206         1503 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.212, whole genome shotgun sequence.
ACCESSION   DAHUVV010000206 DAHUVV010000000
VERSION     DAHUVV010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.212"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            268..867
                     /locus_tag="NK767_002908"
     CDS             268..867
                     /locus_tag="NK767_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCH6695829.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            931..>1503
                     /locus_tag="NK767_002909"
     CDS             931..>1503
                     /locus_tag="NK767_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage abortive infection AbiH family
                     protein"
                     /protein_id="HCH6695830.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT          529 a          232 c          299 g          443 t
ORIGIN      
        1 gaattttcag aatagaataa cttaaaagta ctgacaaaat tttttaaagg ggcaatatta
       61 atgttctgag ataatcaaat cctaccgaaa atatcaaaaa tccaatgtga cacaatcaga
      121 ttgtgtctca ttcgagggaa tcacacaatc ccacgtggct ctaggcagaa ttgaattttt
      181 gcttggggct ttttccttat attgattttg aatgagactt attatatgtt ataatgcaat
      241 taagtatcat tggagagggc gaagaaaatg aagacggtag aacctattcg agacaaaaaa
      301 aagatcgatg cgatgaaggc aatcttggct tcgggaaaat atggccaacg aaatttggtt
      361 cttttttcga ttgggatcaa caccgcctat cgcatttctg atttgcgtca attgaagctg
      421 gccgacgtgc tggaaatttc acgcggtcgc gtggtggtca aagatcgatt agcgatgaag
      481 gaacaaaaaa cagctaaaca taattcggtc tatatttcca acaaattgcg aaaaacgatt
      541 ttggagtacg ttcaacagga atttcctgat caactccttc agcaagattt tgacggttat
      601 ttgttcccta gtcgaaaagg gacgaatgaa ccgctcaacc gccaaagtct gtggcgaatc
      661 attcatgatg ctggcacggc aatcggattg aaagacattg gcccacattc catgcgaaaa
      721 acgttcggtt actttctcta taaacagggg accaaaacag aaatcattca gtccttgttg
      781 aaccattctt ctcagcgcga aacattgcgg tatattggta ttacacaaga agacaaagat
      841 acagctgtaa aaagcttaga tttataatat aaaagttaag tttaaacatg gatagtttta
      901 aattagtaga ttgagtgttg ggaggaaaga atgagtacat tatatattat aggaaacggt
      961 tttgatttaa gtcatggttt aaaaactaga tacactgatt tttcaaaatg ggttgaaaaa
     1021 agtcatagtg aaatattcga taaaatagac acaatgataa atgaaaataa tgaggtgaga
     1081 agtacagctg atgataagat aggttggttt aattttgaaa gtagtttgca tgagttaatt
     1141 caaagtccag tctttgaaga ttatgtagaa gagtctaagg attatctcat aggttatgga
     1201 catgaagatt ggtcagattc tgcccatcat gattttcaaa atatggttta tgattatgtt
     1261 gtttctctag aagaattaca acaacttttc aaggaatgga tacgcaaaga aatacagccg
     1321 acaatagaac ataaaattcc aaaataccaa ttttcgtccg atgtactatt ctttacattc
     1381 aattatacag atgttttgga aattctatac aagcaaccga aagaaaacat atatcatatt
     1441 catggcagtt atgacgatat aataatcggc cactccgaga attttgacaa ctttatagga
     1501 gat
//
LOCUS       DAHUVV010000207         1481 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.213, whole genome shotgun sequence.
ACCESSION   DAHUVV010000207 DAHUVV010000000
VERSION     DAHUVV010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.213"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            38..577
                     /locus_tag="NK767_002910"
     CDS             38..577
                     /locus_tag="NK767_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCH6695831.1"
                     /translation="MNKQELIDKLASLVGSIEDFKGISTFHDGKYAGLEHALELIKQL
                     DEPQKPFVPKFVADYIEEYRGGGATLYEMMDSSEDEVYTWLFGNPRIKTENERQLLFA
                     QAYAVGYEVEKEPLYEVIIGNLFLVKKFNNRNDSCFVNKFELCWWEKSAYQLTEPEIK
                     AIDERYWPFAVPVEEVAEG"
     gene            574..960
                     /locus_tag="NK767_002911"
     CDS             574..960
                     /locus_tag="NK767_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695832.1"
                     /translation="MKSLQQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLES
                     EDLRQIEKSIQLNDNQQIVFDFLKNEFKRNDLQYTLWSFTEDVYERLEIGVSSLPYVK
                     AWGKLKEKQQFEVLTAFAQWGLEQEE"
     gene            963..1157
                     /locus_tag="NK767_002912"
     CDS             963..1157
                     /locus_tag="NK767_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695833.1"
                     /translation="MKAYTIKFYGNKTFVYILSGLRLDFRGYSKDDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEA"
     gene            1157..1444
                     /locus_tag="NK767_002913"
     CDS             1157..1444
                     /locus_tag="NK767_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695834.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKEPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAVIRIENTMDCDKELAKSKANIAVVRLVDMKAI"
BASE COUNT          547 a          218 c          327 g          389 t
ORIGIN      
        1 tataaagaga tattagacga atacatggag gacagcgatg aataaacagg aattgattga
       61 taaactagca agccttgtag gtagcataga agactttaaa ggtataagta cttttcatga
      121 tggaaaatat gcgggtttag aacacgcatt agaactaatc aaacaactag acgaaccgca
      181 gaagccattt gtgccgaagt ttgtggcgga ttatatcgaa gaatacagag gcgggggagc
      241 aactttatat gaaatgatgg acagttcaga agacgaagtt tatacatggc tttttgggaa
      301 tcctcgaatc aaaacagaaa atgaacgaca actgttattt gctcaagcat acgctgtagg
      361 ctacgaggtc gagaaagagc ctttgtatga agttatcatt gggaatttat ttcttgtcaa
      421 gaagtttaat aacagaaatg attcctgttt tgttaataaa tttgaattat gttggtggga
      481 aaaatctgct tatcagctaa cagagccaga aatcaaagca attgatgaaa gatactggcc
      541 gtttgctgtg ccagtggaag aggtggcgga aggatgaaaa gtttgcaaca actcatatct
      601 gaaaaagaaa gcgaactcca aaatttagaa gatagtttgg gattggggtt tccaattgtt
      661 gagcaagcta aaatgactca aatcagtcat ctacgacttg aatcggaaga tttaagacaa
      721 atagaaaaat caattcaact taacgacaac cagcaaatcg tgtttgattt tctaaaaaat
      781 gaatttaaaa gaaatgatct tcaatatacg ttgtggagtt tcacagaaga tgtctatgaa
      841 agattagaaa taggcgttag ctcgttacct tatgtaaaag catggggcaa actgaaagaa
      901 aaacagcaat tcgaagttct aacagcattc gctcaatggg gattagaaca ggaggaatag
      961 aaatgaaagc atacacaatc aagttttatg gaaataaaac ctttgtatac atcctaagcg
     1021 gtctcagact tgactttaga ggatattcta aagacgacct tttagaatcc ttatatggtt
     1081 acggatattt aaatgaagat gaactaataa gcttagagaa atttacaaat gcttggacga
     1141 ttgaggagga agcataatga aactaaaaga cggattttac gctagtagcc acggcatcgg
     1201 cggtttaatg ctagatatgc cgacaaagaa ccctaaaaca cgtaaggaac caaaattcaa
     1261 agtcggtgac atggttcgct gcgaagcaga aggattcatc tatccatttc gtggatatgt
     1321 agaacacgtc tacaatcatt cagcagttat tcgtattgag aatacgatgg actgtgacaa
     1381 agagctagcg aagagcaaag cgaatattgc agttgttcga ttagtggata tgaaggctat
     1441 ataaacagcg aaaaaatgta cttctaagag aaaaatttat t
//
LOCUS       DAHUVV010000208         1405 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.214, whole genome shotgun sequence.
ACCESSION   DAHUVV010000208 DAHUVV010000000
VERSION     DAHUVV010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.214"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            20..430
                     /gene="spx"
                     /locus_tag="NK767_002914"
     CDS             20..430
                     /gene="spx"
                     /locus_tag="NK767_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCH6695835.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            444..800
                     /locus_tag="NK767_002915"
     CDS             444..800
                     /locus_tag="NK767_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCH6695836.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          517 a          209 c          263 g          416 t
ORIGIN      
        1 attaaaaagg agtgagaaaa tgataaaagt atatacttcg cctagctgta cttcctgtag
       61 aaaagcaaga gcatggttgc aagcgaatca acttgaattt gaagagaaga atatttttgc
      121 cgaaccgtta acagaaagtg agatcaaaaa gatcttggca gcgactgaag gaggagttca
      181 agatattatt tccactcggt cgaaaattta cgaaaagctg ggcatagatt ttaacgaatt
      241 aactttaaag caaatgatcg tcttattcaa agaatatcct tcattattga gacgacctat
      301 tttgattgat gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc ggtcatttgt
      361 ccctcgtgaa attcgatgtg ttgccagaaa tctgatctat ttacaaaaca ataacatctt
      421 aaattcatag ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg cactgtgtaa
      481 caagtctgaa ggcgaaacaa tggttagaac accatcaatt gccttatcaa tttgttgact
      541 tagaagaacg cgatctaagt acagaagaag cagaagaatt atgcagtttt gaagaagtgc
      601 aagcagaaca gctatttgcg acttggtctg aggcttttca caacattgcg attgaccttt
      661 cctgtgtagg aaaaaaacaa ctgatttttt tatgcacaca acataccaat ctcctcagac
      721 gtcctttgat tattatcgat gaagttctat ttattggata caatgagcag ttgatggaac
      781 agcttatctt aaataaatag actttaactg ttttaatcaa gtcactgagc atttcataca
      841 gttgtaacag tcgaacactt agcatcaagc gtgttatgtg ttcttttttt gactttattt
      901 ttctactata gagacatgct caatttatgt ggtcaaaatt caaatctagg tggggatata
      961 gagttaaaaa tatattgaca tgtgtaaatt tacatgatat attcatttct gttgttttaa
     1021 gtagcaaagc aataaaatag tattttaaaa aacatataaa atagttgttg acatgttaga
     1081 aatgacatga taagataact gagttgttaa aaaagaacca cttgtgaagc ttgattctat
     1141 cgaaaaaaac ttcaaaaaag ttcttgacaa ataacaaatg ataagttatg ataaatgagt
     1201 tgctgtaaga tttcgaaaaa cgaaatcaaa aaaactttta aaaaagagtt gacaattact
     1261 tgtctctctg atatgatata aaagttgcta cggcagccaa aaacgaatcg gggaacactc
     1321 ctgatgaagt agacctttga aaactgaaca aagtaagaca aaccaaatgt gtagggcgtc
     1381 ttgattcaat tcaagacaac aaaca
//
LOCUS       DAHUVV010000209         1380 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.215, whole genome shotgun sequence.
ACCESSION   DAHUVV010000209 DAHUVV010000000
VERSION     DAHUVV010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.215"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            50..982
                     /locus_tag="NK767_002916"
     CDS             50..982
                     /locus_tag="NK767_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HCH6695837.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1054..>1380
                     /locus_tag="NK767_002917"
     CDS             1054..>1380
                     /locus_tag="NK767_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695838.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVN"
BASE COUNT          606 a          160 c          219 g          395 t
ORIGIN      
        1 ttcaaatata gaagaaactt catcttctga aataaaaaat aagctttctt tgttaaaatt
       61 aaaagaaaag gaatatatta ttgacgatgc tgtttctgtc cctcttattt taaataaacg
      121 agagaaaaat aatttaaaga aaaaaatgct cattccattt gattcagaag tgaaaggatt
      181 actcaataaa ctaactattt ctaatataga ctcgactagg gaaaaaatca taaaaatatt
      241 tgataaaatt aataatttat tcaagaatga caatgctgaa ttaaggaagg gtcttttaga
      301 gtttaaactt caagaactag aacttcatta ctcttattta gtgaaaatta ccgaagaaaa
      361 agaacaacaa aaagcaataa aagaacagat gatagaagag gaaaaagttc gtagagaaat
      421 tgatcgtgaa aagaaacgta ttgataaaga acaacgtcaa ttcaattctg aaatttccaa
      481 attaatcgca tatatgcaaa aagctaatgc agatgtggaa aaagaattat atgcaaataa
      541 aattcaagaa ctggaagaaa aattaaaaga attagaagtt atacgtgaaa atgtattaca
      601 aagagaatta aatactagag ccggttatgt gtatgtaatt agtaatattg ggtcttttgg
      661 tgaagacata tataaaattg gtatgactag aagattagaa cctatggata gaataaaaga
      721 attgagtagt gcatcggttc catttgaatt tgacgttcat gcgatgattt tttcagagga
      781 cgctccgtct ttggaaacta aattgcataa tcattttaga aaacaagaag ttaataaaat
      841 taatcaaaga aaagaatttt tcaaagtttc actagacgaa atagagaaag ttgttttaga
      901 aaattataat ggaacagtaa catttacaaa attagctaaa gctgaacaat atagacgatc
      961 tttagaattg tcaaaagatt aaatatataa aaagccttcg ggctttttct tttaacaatt
     1021 aataggaacg tatattcgaa aggaatagaa ataatgtggt tagaaaaaat aaatgacaaa
     1081 aaatttaagt acaccgaacg ttatacggat cccttaactg aaaaaaagag aaaagtgtcc
     1141 gttactttat ctagcaattc acgccaagct tggaatcaag ctaacttact tttgaatgaa
     1201 aaaatcgcag aaaaaataaa acgaaatgaa gaattaccac taacttttgg agaattaaaa
     1261 actaaatggg atgaaaaata caagccaact gtaaaagaaa gttcctatcg aacaactcag
     1321 gtttatctat cattaatttc aaaatatata aaagatgatg tacttgtaaa aaacgttaat
//
LOCUS       DAHUVV010000210         1368 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.216, whole genome shotgun sequence.
ACCESSION   DAHUVV010000210 DAHUVV010000000
VERSION     DAHUVV010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.216"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            82..>1368
                     /locus_tag="NK767_002918"
     CDS             82..>1368
                     /locus_tag="NK767_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HCH6695839.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DAHUVV010000211         1246 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.218, whole genome shotgun sequence.
ACCESSION   DAHUVV010000211 DAHUVV010000000
VERSION     DAHUVV010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.218"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            200..742
                     /locus_tag="NK767_002919"
     CDS             200..742
                     /locus_tag="NK767_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695840.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="NK767_002920"
     CDS             898..>1246
                     /locus_tag="NK767_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HCH6695841.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAHUVV010000212         1244 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.219, whole genome shotgun sequence.
ACCESSION   DAHUVV010000212 DAHUVV010000000
VERSION     DAHUVV010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.219"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            13..1020
                     /locus_tag="NK767_002921"
     CDS             13..1020
                     /locus_tag="NK767_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCH6695842.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1032..>1244
                     /locus_tag="NK767_002922"
     CDS             1032..>1244
                     /locus_tag="NK767_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HCH6695843.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEM"
BASE COUNT          490 a          160 c          239 g          355 t
ORIGIN      
        1 gaggagatat aagtgtttaa aggaaaaaca atattagtaa ctggcggaac tggttccatt
       61 ggaagtgaaa ttgttcgtca gcttttaaaa tatgaaccaa aagctatcag aatttatagt
      121 agaggtgaag aaaaacaatt ttatatgcaa caagagctct caaatcacac taatttgaga
      181 tttttaattg gtgatatcag agactctcaa aggattgatt atgcatgtcg tgatgtggat
      241 attatttttc atgctgccgc aatgaaacac gttccagcaa gtgaatataa tcctatggaa
      301 gctgtaaaaa caaatgtgat gggaactcaa aatgtaatag atgcagcgat caaaaataat
      361 gtaaaacatt ttgtaggtat cagtacagat aaagtagtga gtcctactaa caccatgggt
      421 gcgacaaagt tattgagtga aaaattaata atagctgcca atcagtataa aggaaaaaca
      481 gaaacaatat tttcttgtgt aaggtttgga aatgtcatgg ggtcaagtgg atctgttatt
      541 cctcttttta gtaaacaaat aaaaaacaat aaagatatta ctttgacaga tgttgatatg
      601 acacgattta tgatgtcaat accggatgct gcaagattag ttctgtctgc tgctatgaat
      661 tcaaaatttg gagaaacata tgtcttaaaa atgccggcaa tcaatatttt aactttagct
      721 caatcattaa taaaaatata taatgataca catgatgaaa aatatagagg tgaaataaaa
      781 gttgtaggta tcaggccagg agaaaaatta tatgaagagc taatgactac agacgaaatt
      841 gaacgctcat atgaaaatga taaaatgtat atcattccac cacactatca tgctactgac
      901 gaactgtaca aagattatga aaaaattaaa atcactgcat actcttcaga tgcaacagaa
      961 ttaatgagtg gacaggaatt tattagaatc gcaaaagaga ataatgtcgt gacttggtaa
     1021 ggagttttat gatgaaatca gaagtatatg taattgcaga aattggtgta aaccataatg
     1081 gaagtctaga tttagcttta gaaagtattg ataaagctaa agaatgtgga gctaacgcgg
     1141 ttaaatttca aacatttaaa acgaataagc tagtttccaa aaaggctcca atggcaaaat
     1201 atcagaagga taatcttaaa gaggacagtt ctcaatttga aatg
//
LOCUS       DAHUVV010000213         1235 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.220, whole genome shotgun sequence.
ACCESSION   DAHUVV010000213 DAHUVV010000000
VERSION     DAHUVV010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.220"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..>1235
                     /locus_tag="NK767_002923"
     rRNA            <1..>1235
                     /locus_tag="NK767_002923"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          324 a          280 c          375 g          256 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc cattt
//
LOCUS       DAHUVV010000214         1235 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.221, whole genome shotgun sequence.
ACCESSION   DAHUVV010000214 DAHUVV010000000
VERSION     DAHUVV010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.221"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..113)
                     /locus_tag="NK767_002924"
     CDS             complement(<1..113)
                     /locus_tag="NK767_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCH6695844.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSS"
     gene            complement(110..529)
                     /locus_tag="NK767_002925"
     CDS             complement(110..529)
                     /locus_tag="NK767_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCH6695845.1"
                     /translation="MEKYLNTLSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(599..739)
                     /locus_tag="NK767_002926"
     CDS             complement(599..739)
                     /locus_tag="NK767_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCH6695846.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            complement(732..1079)
                     /locus_tag="NK767_002927"
     CDS             complement(732..1079)
                     /locus_tag="NK767_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695847.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKSGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
BASE COUNT          357 a          266 c          172 g          440 t
ORIGIN      
        1 gaactacctt cgtttgcgcc caaattgaat tcattgttga tcgagtaagc aaaggcgttt
       61 attggcaata aaaaaagagc tataatagct cctacaaaga ttttcttttt catttggttc
      121 ctcctcggtc tttattttca gtatcatttc gtatttgttg gaatacctct tttaacttat
      181 ctggcaaagg aaggaatacc gaaatgtttt ctaaaaaact aatcccttca tttgcaacat
      241 agaacataat aactatttct cttagaggta tagaattccc aacgattttc tgtacgtcaa
      301 ccgcaacggc aatcacaacg aagattaaga ctttcttcaa caatccttcg aaacctatct
      361 cactacttat ttctttcaat ttccatgcct tagcaagtcc agttaagaag tccacaccca
      421 caagaaaaat taaaacatgt agtatattgt ccatccctcc caataaacca acaattgtcc
      481 cgcctatcac ccctgtcatt actgagagtg tatttaaata tttttccaat atctatttct
      541 cctttatgca aagaagcgca ctcgtttgag tacgcttttt atttaacttt gtgcttaatt
      601 aaatttcatt tccagtaatc tgcttatatt ggtcttttgt taaacagttc atgtttacat
      661 agtcaagtaa atcttggtcc gtataacatc cccaatcata aaattgtttt atattttcaa
      721 agcccggaaa actattcatt acttttccct ccattcttaa tacttgcgac ttccatcgtc
      781 aattgagcta acatttgttg cattttttct tgttcctgta ccgatatcgc caattgtttt
      841 gtcagtagag catttaattg cttcaactga tcgacatctt caatatcgtc aaccgctgtg
      901 ccactcttct cttcttcagt agctaactca acccactgtt ttttatcaaa atcaaatctt
      961 ggtctccagt taggaactgg tggtttaatc tctgtacagt tttcagggat attttcttga
     1021 ttgttcaaaa tgatttgctc gaatccgtaa ggcttgattg atttgtaaac tactttcatt
     1081 agatttcctc ccttaaattg aataagtgat aacaaatgaa taagctgatc cataacttga
     1141 atttcttctc catttaatgg ctccgtctgc gccaatagat agttgagcac tgttcaaagt
     1201 agaacggtct atcgagccaa ccagttgctc aaaac
//
LOCUS       DAHUVV010000215         1164 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.222, whole genome shotgun sequence.
ACCESSION   DAHUVV010000215 DAHUVV010000000
VERSION     DAHUVV010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.222"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            307..1101
                     /locus_tag="NK767_002928"
     CDS             307..1101
                     /locus_tag="NK767_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HCH6695848.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKGPSEKRKLNISDYTTQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKREILLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISAINSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
BASE COUNT          467 a          154 c          170 g          373 t
ORIGIN      
        1 taaacttcgt agcctccact acaactttta tttttataaa caaaaaaggc tgtgacaaaa
       61 gtcttgtcac aacctcttta atcataagat tcaattaagc ttcttgagct actggataaa
      121 cagacacttc aattatactg tttcctgata agctctcaat gcttctgaac ccatgatatt
      181 agtgcttttc ttctattatt aaatatcttg taattttcta ttctttctaa aaagtatggt
      241 caaagtatgg tcactattga tagaatatta atagtagtag aaagctgata tctaggagga
      301 actaatatga gtaaacaatg tgcaaagtgc ggaggtaaaa ttggcttaac ttcatataaa
      361 atcaaagata aacaaattgt ttgcggcaat tgtatgaaaa aagctggtta cggtatgact
      421 actccttata aaataataag atctttgaat ttagaagatt tagataaggg tccatcagaa
      481 aaaagaaaat taaacatatc tgattatact acacaaaaca atcatgatat aggtaatgat
      541 agaatgagaa ctactgaaga gatggtagaa ttttgtctta aatacggcta tggtaaagga
      601 atgactaaaa aatggacaac tcatcatttt aacttaatat cagatcaact taacagtgac
      661 gaatacgtaa tcttctgctt tgtaggattg cataactata tctcgtcaac taagcatgac
      721 aataattatg catacgcatt aactaataaa cgtttaattg tagctcaaca aaaaatgata
      781 ggcaataatg tacaaagtat tatattagac aatttaaata atattagtaa aaaaagagaa
      841 atactgttgg gtacattaac tattgatact cttggtaaag tattgaatgt tgcagttgat
      901 aaagatactg ttgataggat aagtgattct ttaaacgaaa ttatttataa tttaaaaaga
      961 agaaatattt ctgctataaa ttccaataca tcaacaattt cctcagcttc agaaataaga
     1021 aaatataaag aactattgga tgacggaatc attacagaag aagaatttaa taaaaagaaa
     1081 caagaattat tagatttata atatctatca aaaaagatga gcatttaatt tagctcatct
     1141 ttttctattt acaatacaaa catt
//
LOCUS       DAHUVV010000216         1151 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.224, whole genome shotgun sequence.
ACCESSION   DAHUVV010000216 DAHUVV010000000
VERSION     DAHUVV010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.224"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(405..779)
                     /locus_tag="NK767_002929"
     CDS             complement(405..779)
                     /locus_tag="NK767_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCH6695849.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(802..>1151)
                     /locus_tag="NK767_002930"
     CDS             complement(802..>1151)
                     /locus_tag="NK767_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HCH6695850.1"
                     /translation="TKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQMFD
                     KGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGAIKV
                     DKLDYSINYECGF"
BASE COUNT          391 a          216 c          139 g          405 t
ORIGIN      
        1 taggagtgag catttttcga aaaatactaa aaatttttca aaataaaaaa aggcatacag
       61 acaataaact gtctatacgc ctcacagact ctattctata catctaattt tggctgtcct
      121 gcaaaaaata tgtgttagcc tccatcgaaa gatcaaactt tggaaaacat cattataact
      181 ttgcttggta agagctttgc taaaacatga tatgacttgt atacaaaatg tggagtgtat
      241 tccaataaat aggaaggatt acaaaatttg aaaccaaata agtaaaaaat actgtcactt
      301 tgaattaatt tgaaatagta aattctcatt aaactattga aatgctgata ccaaaaaatg
      361 aagtactttt cgttcatacc aacttagagc acttcgttat ctatttattt cttcatttgg
      421 cttatctcta taatctcatg atttggtccg tagaaattaa agtaacggat tccattttcc
      481 cagaacggta attccataat atttttatct aataattcat atcctaaatc ttgaattgaa
      541 ttgaatacct catctatgtc atccgtatct aatgctagat gatcaatggc tcctctacac
      601 tcagtagttt tatcttccca tatttctatt atcgttccat tcaactcgaa aaagccgact
      661 ttacttccca cattcatttc ttcattaatc agactaaacc ctaaggattc ataaaagatt
      721 tttgaagctc caaaatcttt tgttggaatt ccaatatgtt gtaaaccatt taatttcact
      781 tacatcatcc cttcaattgt tttaaaatcc acattcataa tttattgaat agtctagttt
      841 atctaccttg attgctccct ccgtaacagt catacttaat gctgttccga ctttatcata
      901 cattctagta cttaaagcaa cattatcaat atataaaaca gctatcgtat catcaacaat
      961 taactttagt tcatattctt tgcctatttc caaaaatagt ggtcgttgca gccccttatc
     1021 aaacatttga ggccaaggat agttcggagt ttttccgaaa gtaagctgat tattaatcaa
     1081 tgaaatacga aattcgtatc cttcttgagt atcactatct acaaataatt ttaaagcaaa
     1141 ctcctttgtt a
//
LOCUS       DAHUVV010000217         1137 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.225, whole genome shotgun sequence.
ACCESSION   DAHUVV010000217 DAHUVV010000000
VERSION     DAHUVV010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.225"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..355
                     /locus_tag="NK767_002931"
     CDS             <1..355
                     /locus_tag="NK767_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695851.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="NK767_002932"
                     /pseudo
     CDS             615..910
                     /locus_tag="NK767_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1137
                     /locus_tag="NK767_002933"
     CDS             985..>1137
                     /locus_tag="NK767_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCH6695852.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          431 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggta
//
LOCUS       DAHUVV010000218         1115 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.226, whole genome shotgun sequence.
ACCESSION   DAHUVV010000218 DAHUVV010000000
VERSION     DAHUVV010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.226"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            283..522
                     /locus_tag="NK767_002934"
     CDS             283..522
                     /locus_tag="NK767_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695853.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1090)
                     /locus_tag="NK767_002935"
     CDS             complement(884..1090)
                     /locus_tag="NK767_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695854.1"
                     /translation="MKQKVVGEVISKLENCVVICVDEYHDVDRNEILEKCGKVVAKYT
                     HVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          360 a          208 c          160 g          387 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttt
//
LOCUS       DAHUVV010000219         1106 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.227, whole genome shotgun sequence.
ACCESSION   DAHUVV010000219 DAHUVV010000000
VERSION     DAHUVV010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.227"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            1..999
                     /locus_tag="NK767_002936"
                     /pseudo
     CDS             1..999
                     /locus_tag="NK767_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          391 a          199 c          222 g          294 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      481 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      541 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      601 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      661 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      721 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt agctgaatca
      781 acttataagt cgttgaaagt agaatttgtc tatcaataca catttgaaac cttacaacaa
      841 ttggatttgg agttatttga ctatgtcaat tggtggaacc accttcggtt gcacggtaca
      901 cttggctacg agacaccggt tggttaccgt aaccagagat tggcgcagcg aatccttgat
      961 aatgagctcg gatgtgctaa cgctagcgag gcagtctaac tttaactgtt agctcctgcc
     1021 gaagatcgtc acatccgagg aggctcattg tcaaggacaa tcggaatagc atacggaaag
     1081 aagttctgca ccttataaaa tttgtc
//
LOCUS       DAHUVV010000220         1100 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.228, whole genome shotgun sequence.
ACCESSION   DAHUVV010000220 DAHUVV010000000
VERSION     DAHUVV010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.228"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            66..1013
                     /locus_tag="NK767_002937"
     CDS             66..1013
                     /locus_tag="NK767_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCH6695855.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          354 a          168 c          235 g          343 t
ORIGIN      
        1 taaattttta gaagattctt cggagcttca agatatctat aattatttat atcggaggta
       61 aaaggatgag ctgttatctt atcaccggtg gggctggctt cattggttct accttagcca
      121 atcatttagg agaagaacat gtggtaatcg tcgtcgatga cttatcaatg ggaaaagtag
      181 aaaatcttga tatgacgaaa aatatcactt ttattgaggg agatgtcgct gagaaatcct
      241 tgatggaaga gatcatgaaa gctaataaat ttgattatat ctttcattta gcagcagttg
      301 ctagtgtggc agattcagtt gcccgaccag tagaaacaca tcgtgtgaat tttgaaagtg
      361 tattaatgat tttagaattg atccgcaagt atcaacctga tttaaaacgg atcgtgttta
      421 gttcttcagc ggctgtttat ggggatgaac cgactttacc aaaaaaagaa gagtccgtga
      481 ttcgcccatt gacgccttat gcggttgata aattcgctgc agaacaatat gtgctagatt
      541 attgtcattt atatgatgta cccggtagcg cagttcgttt tttcaatgtc tatggtccaa
      601 atcaaaatcc gaattctcca tacagtggtg tgatttctat tctcgtagat cgctataaga
      661 aacaaatcgt aggagaagca acttcattta cattatttgg tgatggaagc cagtctagag
      721 actttgttta tatagatgat gtaatccaag cattactttt agttgcgaat gaggaagctg
      781 cactggggaa acaattcaat gttggaacgg gtaaagcaac cacacttttg gaattgattc
      841 atgcaatcaa tgaaattctc ggtgtagaat tgacacttga atacaaagaa gagcgttcgg
      901 gtgatatcca tgactctcta gcagatatta ctaagatcca gtcaattggc tatcaaccga
      961 aatatggtat tttaagtgga atgactaatt atttgaaaac tgaaatcaat taaaacatac
     1021 taagataaga ttagagggta aattcaggct ctttgtcaat aaggactgat gagttttgta
     1081 aaatcaaatc tgggatagaa
//
LOCUS       DAHUVV010000221         1066 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.229, whole genome shotgun sequence.
ACCESSION   DAHUVV010000221 DAHUVV010000000
VERSION     DAHUVV010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.229"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            29..877
                     /locus_tag="NK767_002938"
     CDS             29..877
                     /locus_tag="NK767_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCH6695856.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT          365 a          192 c          185 g          324 t
ORIGIN      
        1 aatcttatta tgaaaatgag gaattctcat gagcgtctcc ataagactac aaatcaaaac
       61 aatgtacaac gacttcagcc caaaagaaca ggctatcgcc gattatattt tagaaaatcc
      121 aagcaaagta agtcatagtc ctattagcaa tttagcaagt gaactgggca ttgctgactc
      181 tacttttttt caatttacaa aaaaacttgg ttataatgga tttaaagatt ttaaaatggc
      241 catgttgatc caagaaaatg atctatctgc tatttctatc cacgaaaata ttcagaaaac
      301 agatactgag ctcactatgg cgcaaaaggt gttcgagtct agtatttcaa ctttaaatga
      361 cacaaaaaag ttgttacaac aaaaggatct aaaaaaagcg gctactatta tcaatgaatc
      421 taaacgtgtc tattttttcg gtgttggcgg ttcagagatt gtagcaactg acgcttatca
      481 taaatttttg cgttccccta tcgcaacttc tcacagcacg gattaccata ttcaattgat
      541 ggaagcttct ttattgacgg aaaaagactg tgctgttttg atttctcaca cagggcaatc
      601 aaaagaaaca atccatattg ctgaaaccgt aagaaaaact ggagcaaaaa caatcgttat
      661 tactagccaa gctaattctt cccttgctaa acttggcgat gtcgttttta tttctatttc
      721 tgaagaaaca gaattccgat ctgaagcttt agcttcccgt atttctcaac taagcatttt
      781 ggactctcta tatgtgattt tgatgtttta caacgaaaaa gaatcacgtc gttctatcgc
      841 taaagttcga catactattt ctgaaatcaa agaatagcac cataacagaa aaagcgaaaa
      901 atccataaaa caaaaaaagc cactaccaaa tatttggtag tggctttttt ggttctgcaa
      961 gggtggtaca gaacatcaat agtcggtggg ttagaacgta tgtcatcgca tctttggaga
     1021 aggttgtatt ggtattattc gagttacttg ttagatgcga taacaa
//
LOCUS       DAHUVV010000222         1061 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.230, whole genome shotgun sequence.
ACCESSION   DAHUVV010000222 DAHUVV010000000
VERSION     DAHUVV010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.230"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(36..992)
                     /locus_tag="NK767_002939"
     CDS             complement(36..992)
                     /locus_tag="NK767_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695857.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DAHUVV010000223         1034 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.231, whole genome shotgun sequence.
ACCESSION   DAHUVV010000223 DAHUVV010000000
VERSION     DAHUVV010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.231"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(169..>1034)
                     /locus_tag="NK767_002940"
     CDS             complement(169..>1034)
                     /locus_tag="NK767_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HCH6695858.1"
                     /translation="SLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLS
                     KTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSG
                     DTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSG
                     IVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNE
                     MHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQ
                     LGLVDNFNNIS"
BASE COUNT          358 a          179 c          120 g          377 t
ORIGIN      
        1 actttgtcat attcatttaa cacactatta ttaccattac ttagacgctc agattctacg
       61 aaacaactca aatcagaaat ttcccaaaaa aattttttgt taaaatccaa tttcctcaac
      121 tcctccaaac tacgtaaatg atatagtctc ataataaaat aaaacctctc agctaatgtt
      181 gttgaaattg tccactaaac ctaattgata ttgcttagga gatacacctg cgtacttttt
      241 gaatactctc gaaaagtgtg aagaatctga aaatcctaaa cctaaagaaa cttcactgat
      301 agtatggttt gatttcaaca ttattttagc cgtagaaatt tttcgcttag agatataatt
      361 ataaatcgta atatgcattt cattcttaaa gctttctctt aatttagttt cacttacaga
      421 aaaataatta gctatttcct ttacagtaat tcttttatag atatttaagc cgatgaattg
      481 aacgacagaa gaaattaagg gagaaagatg ctctatctta gtttttccga tttccttagt
      541 ataataaaca atcccagaat ccctcacttt taagacctca ggtaacgata cagctaattc
      601 gttttcttgt atcaaagcat ctctactttg atacgcttct attatatcca ttcctaatgt
      661 tatagacgtt tgtgctaact tttcaaaaac aatgatagaa taattttttt cagaccgaat
      721 cgtatctcca ctaacacttg gaattattcc attactcaga ttaaaaacca tgttttctaa
      781 atttcgaata tctccatttt taacatattc cagtatttta ttttcataaa gaaacagata
      841 tatttctgga tcgtaatttt gtgataacag attatctatt ttgatttgct cgatagtttt
      901 acttagattt tctgtatatt caattaacgg ttcatggaat agatcttcta tttttccagt
      961 aaaaaaataa tgaactaaaa tcagaatatc tcgaatgtct cctaaagaat acaaagtcaa
     1021 attttgcaat gaat
//
LOCUS       DAHUVV010000224         1017 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.232, whole genome shotgun sequence.
ACCESSION   DAHUVV010000224 DAHUVV010000000
VERSION     DAHUVV010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.232"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(602..675)
                     /locus_tag="NK767_002941"
     tRNA            complement(602..675)
                     /locus_tag="NK767_002941"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Ile,seq:cat)
     gene            complement(865..>1017)
                     /locus_tag="NK767_002942"
     CDS             complement(865..>1017)
                     /locus_tag="NK767_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCH6695859.1"
                     /translation="VGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWY
                     LNIPKR"
BASE COUNT          339 a          178 c          175 g          325 t
ORIGIN      
        1 gactgacgtg ccggacgcac gtagcgatca tcggcgttat gactatataa agtatacttt
       61 cgttgatcca gaaaggtata aagattaaag taggctacct aaaaaggtag cccggaacga
      121 attttatcac catacttgtg aaaggagata ttttttaagt tagtattaag attgtgtaat
      181 atgatgatat ctatatttta tagtatcagt gctataaaat caaaaataag tcactaatta
      241 actaccactc caattgtaag ccttttttct cacttttttt caaaaatatg gtatgctttt
      301 taatggcttc aaatataaaa gagtttaaag cgtaacacac ttatggggaa gtggtttggg
      361 gtgcgcttta aactcttctt tattattatc tcacaattta acccaaatgt ctttctattt
      421 aaaaatcaaa gtaaaacttt tcaaatatac agaagtataa ctatgtgaaa catcctttca
      481 ttaatccata aaaggataca taaaaaagcc actcatttga gtggcaatga agaaaagctt
      541 tagcttgtat aatactcttc aaaaaaattc taacacagaa cgattcaaat ggctacgtta
      601 atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg ctctgaccaa
      661 ctgagctaag ggtactggtt gttgccacat aaagccataa acaatcaacc agtagaatgt
      721 gtggcaacaa acctgttatc gcatagcttg gagtgtgact atttatgggt gatagtgaag
      781 atatgcgata acatcactat tttatcgaat aatttttata gttgtcaata tagttatgta
      841 ctgctcctca acgaggagct atttttatcg tttaggaata tttaaatacc aacgtttgtc
      901 atggaaatct tgcgcaccgc ctttagtgtt tccctctgga tcattcgttg cccgcatcat
      961 tacatagact ttcttactag gaaaattacg catattgaaa gatacatgat aaccaac
//
LOCUS       DAHUVV010000225         1004 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.233, whole genome shotgun sequence.
ACCESSION   DAHUVV010000225 DAHUVV010000000
VERSION     DAHUVV010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.233"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..870
                     /locus_tag="NK767_002943"
     CDS             <1..870
                     /locus_tag="NK767_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCH6695860.1"
                     /translation="LIEVNKLLKKESFSIVHNQVYFGGGINLWLAKKNGIRQRIAHSH
                     ATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLEL
                     YSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILV
                     GEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQ
                     AAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSY
                     TTEQMESIYSRDH"
BASE COUNT          388 a          112 c          190 g          314 t
ORIGIN      
        1 ttaatagaag tgaataagtt actaaaaaaa gaatcttttt ctatagttca caatcaggtt
       61 tattttggcg ggggaattaa tttatggtta gccaaaaaga atggaatcag acagagaatt
      121 gctcatagcc atgcaactga agatggaaaa tctcaaaata ttgtaatgaa tgtattgcgt
      181 aaatttttaa ccaaattgtt gttacaaaat gcaacagact accttgccgt tagtcaagaa
      241 gctggtgaaa gtctgtttca aaaccatccc tttgagatag ttcataatgg tattgattta
      301 gagctttata gtaaaaattc agaagctaag gttaataaga gaaaagaatt ggatatctca
      361 atgagtactt ttgttgtggg aaacattgga cgattagaga aacaaaaaaa tcaatcttat
      421 ttgttagaga tttttttaga aatactaaaa atgaattctg attcttactt gattttagta
      481 ggtgaagggt cacttagaaa taaactgaaa gccaaagcag tagaattgga tatagagaaa
      541 agagtaaaat ttttaggaga aagaaacgat attcccgagt tattatctgt gatggatgtt
      601 tttgtaatgc cttctcttta tgaaggtctg ccaatggttg gattagaagc acaagcagca
      661 aaattaaaat tagtcttagc agatactatt tccaaagata caaagttaac aaataatgtg
      721 gattttgtat cattagatga aacaccacaa gaatgggcaa aaaaagtaat aaaagagccg
      781 tatgctaata aaataacaca agaactaaag agttatgatg tttcgtacac tactgaacaa
      841 atggagagta tatatagtag agatcattaa aaatgttcaa gatattttag gagtgattat
      901 ttttgaaaat atgttatttg atattggcat acaatgatat tggcaactta aagagattgg
      961 tcaatcgatt gaacgaaaat gcagttttta ttattcatat agat
//
LOCUS       DAHUVV010000226         1003 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.234, whole genome shotgun sequence.
ACCESSION   DAHUVV010000226 DAHUVV010000000
VERSION     DAHUVV010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.234"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            370..1003
                     /locus_tag="NK767_002944"
                     /pseudo
     CDS             370..1003
                     /locus_tag="NK767_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          295 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DAHUVV010000227          943 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.235, whole genome shotgun sequence.
ACCESSION   DAHUVV010000227 DAHUVV010000000
VERSION     DAHUVV010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.235"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..>943
                     /locus_tag="NK767_002945"
     rRNA            <1..>943
                     /locus_tag="NK767_002945"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAHUVV010000228          937 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.236, whole genome shotgun sequence.
ACCESSION   DAHUVV010000228 DAHUVV010000000
VERSION     DAHUVV010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.236"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(246..319)
                     /locus_tag="NK767_002946"
     tRNA            complement(246..319)
                     /locus_tag="NK767_002946"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(283..285),aa:Ile,seq:cat)
BASE COUNT          341 a          150 c          147 g          299 t
ORIGIN      
        1 ttggggaacg ctttaaactc ttctttatta ttatctcaca atataaccca aatgtctttc
       61 catttaaaaa tcaaagtaaa acttttcaaa tatacagaag tataactatg tgaaacatcc
      121 tttcattaat ccataaaagg atacataaaa aagccactca tttgagtggc aatgaagaaa
      181 agctttagct tgtataatac tcttcaaaaa attctaacac agaacgattc aattggctac
      241 attaatgtac cctgtaggac tcgaacctac gaccggacgg ttatgagccg tctgctctga
      301 ccaactgagc taagggtagt ggttgttgcc acataaagcc ataaacaatc aaccagtaga
      361 atgcgtggca acaaacctgt tatcgcatat cttggagtgt gactatttat gagtgatagt
      421 gaagatatgc gtctccatat atgtatggtt gagacagata aacatgtatg aaaaacgttg
      481 ttaaatcaac gtttttttat tttggtcaag gagagaaaag tagagaaata caaaattttt
      541 tgcattgatt ttgcattcaa aaaaacattc aaacatttag aaatccccaa tacgtattca
      601 ggaaaatcta ttttaaggtt tacaaaaaga acttttgttc gtacactctt tttgaggtga
      661 acttttatgc tgatggaagg aaaaacacaa ttatggttta agcatgttcc ttcaaataag
      721 ttttttaatt ataaactcta gagaaattaa ttcatcctaa cataaaataa ttgttcactc
      781 aaaagttttt ctagaacaat taaaacagca ggcttcacat tcgctataca tttgtaagaa
      841 gtaataattc ctgtactaaa aggcataatt gctttaatta ttttttgact tctcttcaaa
      901 cacaagaagt agttgtgtaa taattgcaga aataagg
//
LOCUS       DAHUVV010000229          926 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.237, whole genome shotgun sequence.
ACCESSION   DAHUVV010000229 DAHUVV010000000
VERSION     DAHUVV010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.237"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..254)
                     /locus_tag="NK767_002947"
     CDS             complement(<1..254)
                     /locus_tag="NK767_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCH6695861.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
     gene            complement(254..517)
                     /locus_tag="NK767_002948"
     CDS             complement(254..517)
                     /locus_tag="NK767_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCH6695862.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            751..888
                     /locus_tag="NK767_002949"
                     /pseudo
     CDS             751..888
                     /locus_tag="NK767_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
BASE COUNT          294 a          180 c          130 g          322 t
ORIGIN      
        1 gtccttgctg aataaatttc aaccacgtgt tcatcttcat ctaccttata aacaacccgg
       61 tgttgtctat tcaatcttct agaataccta ccttcatgtg taggttttaa cttctcaaat
      121 gattgggtag ggagataggg atcttccttt aaagtctgaa taaccttctc aaattgaggt
      181 ttcaaattag actgtttgat tttctttaaa tcagctttag ccgagttttt aatcatcaca
      241 gaatagttat tcattataag ttatcccaat caatttcatc aacattagta aatccactat
      301 catctttttc tcgcttacga acaacaccca aagtgcctat ttgctcaagt aatagcgttt
      361 ctttgattgc tttccaatca tccaaaccaa taagcaccgc attattttca ctgcgctcac
      421 taatgatttc aatttccgta tgattctcat ttacatcttt caacaactta taaaaatttt
      481 tccgtgctga gctaggattt aagactgttt ttgccatatc tactccccct ctaatttata
      541 tctccattct aacgtactta attaagtacg taaattcgat tgttttgttt aaattggagt
      601 accaaaacag cattgtaaag tgttatccgt ttcctttata cccgtggtgc tagttaattt
      661 tttcctagag gaaaatgtct aaacatgttg gtaaatcaac aaaaaaacgc atagagtagt
      721 ctcaaaactc ccaagaaatg aggactacat atgcgttcat caaaatatac tgaacattat
      781 acagatactt ttataacatt caaggaaatt atgagaactg tttctaacat ataccacaat
      841 tgtgtaccag ataagattaa aaaccgaaga aatactgatc aactgaagta acgcgataca
      901 gtgattattg cttgtgttat atgggg
//
LOCUS       DAHUVV010000230          897 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.238, whole genome shotgun sequence.
ACCESSION   DAHUVV010000230 DAHUVV010000000
VERSION     DAHUVV010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.238"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            4..>897
                     /gene="bsh"
                     /locus_tag="NK767_002950"
     CDS             4..>897
                     /gene="bsh"
                     /locus_tag="NK767_002950"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCH6695863.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAID"
BASE COUNT          305 a          123 c          162 g          307 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact attactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tggactaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgat
//
LOCUS       DAHUVV010000231          887 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.239, whole genome shotgun sequence.
ACCESSION   DAHUVV010000231 DAHUVV010000000
VERSION     DAHUVV010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.239"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(74..146)
                     /locus_tag="NK767_002951"
     tRNA            complement(74..146)
                     /locus_tag="NK767_002951"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:His,seq:gtg)
     gene            complement(149..222)
                     /locus_tag="NK767_002952"
     tRNA            complement(149..222)
                     /locus_tag="NK767_002952"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(188..190),aa:Trp,seq:cca)
     gene            complement(229..309)
                     /locus_tag="NK767_002953"
     tRNA            complement(229..309)
                     /locus_tag="NK767_002953"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(273..275),aa:Tyr,seq:gta)
     gene            complement(311..386)
                     /locus_tag="NK767_002954"
     tRNA            complement(311..386)
                     /locus_tag="NK767_002954"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(351..353),aa:Phe,seq:gaa)
     gene            complement(422..494)
                     /locus_tag="NK767_002955"
     tRNA            complement(422..494)
                     /locus_tag="NK767_002955"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(459..461),aa:Asp,seq:gtc)
     gene            complement(504..576)
                     /locus_tag="NK767_002956"
     tRNA            complement(504..576)
                     /locus_tag="NK767_002956"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(541..543),aa:Val,seq:tac)
     gene            complement(592..663)
                     /locus_tag="NK767_002957"
     tRNA            complement(592..663)
                     /locus_tag="NK767_002957"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(628..630),aa:Glu,seq:ttc)
     gene            complement(678..767)
                     /locus_tag="NK767_002958"
     tRNA            complement(678..767)
                     /locus_tag="NK767_002958"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(731..733),aa:Ser,seq:gga)
     gene            complement(807..880)
                     /locus_tag="NK767_002959"
     tRNA            complement(807..880)
                     /locus_tag="NK767_002959"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(844..846),aa:Asn,seq:gtt)
BASE COUNT          248 a          243 c          203 g          193 t
ORIGIN      
        1 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
       61 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
      121 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
      181 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
      241 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
      301 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
      361 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
      421 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
      481 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
      541 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
      601 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
      661 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
      721 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
      781 ctcaatactc tttctcaaaa tgagaactcc ggcagtagga ctcgaaccta cgacatcatg
      841 attaacagtc atgcgctact accaactgag ctatgccgga ataacat
//
LOCUS       DAHUVV010000232          859 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.240, whole genome shotgun sequence.
ACCESSION   DAHUVV010000232 DAHUVV010000000
VERSION     DAHUVV010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.240"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            124..825
                     /locus_tag="NK767_002960"
     CDS             124..825
                     /locus_tag="NK767_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCH6695864.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          312 a          150 c          145 g          252 t
ORIGIN      
        1 ataataacaa attatatttg taaagtatta aaacttgttt ctaatgttgg tatgttaaaa
       61 ctttacaaat tctttaaaaa aagtgacaat aaatatagtg aacaataagg agaggacttt
      121 ttaatgcgtg ttaataaaat cgattttcac ttttcaaaac tcagatatca acctgatttt
      181 gaacatttct cggatgaaga gttccaacag atcaaggaac atactgttct tcgttcttat
      241 aaaaaaggac agatcctttt cgacgatggt gatcccagac aacggttgta tatcttgacc
      301 aaaggactcg ttcgtttaga acgttatgat gaaagtgcaa cttattacta ctatgactat
      361 gtgaatacaa ataacctttt tcctatgtca ggaatgtttg gagaagagac ctacacttat
      421 acagctcaag cgatgactga tattgaaaca ttctaccttc ctgtttccta ctatgaaaaa
      481 ttagcacgaa aaaatcccga acagttgatc tatatgatac aaaaaatttc taaagtagtt
      541 gaaatacatg aattgcgtat ccaaatcgga ttgacctcta gcgcctttga ccgggtaaaa
      601 caaaatttat tcattttgaa agaagaattg ggcgtaccaa caaaagatgg tcatatcacc
      661 attccttatc cgatcacctt aaaagaatta gcagtgaata gcggcacgac acgtgaaact
      721 gctggacaag taatcaaaca actacgtgat tccgggctgt tagattatga caaaaagcgt
      781 tttactttct gtaagacagc tactactcca gctatgagag aataacgcga acaaaaataa
      841 gcagtgaccc tgataaata
//
LOCUS       DAHUVV010000233          856 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.241, whole genome shotgun sequence.
ACCESSION   DAHUVV010000233 DAHUVV010000000
VERSION     DAHUVV010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.241"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            134..772
                     /locus_tag="NK767_002961"
     CDS             134..772
                     /locus_tag="NK767_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCH6695865.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          279 a          136 c          132 g          309 t
ORIGIN      
        1 aagtagtata atcaaaatga atatataaga taaattttta gcaatacaaa tatccataaa
       61 aaaacattgc ctattttaac tcttcatgta tactgactct atcttcaaaa actgaatagt
      121 aggagttatt atcatgaata acattttagg aaaaaaaata gaacttaaaa aagggatatt
      181 gggcactatt gtcgttgtac tcggtttgtt agggttgcta tttgcgccat ccattattag
      241 cattttttct ttaactatca ctggatttat agcatttaca cttgtttttg gtaaaaaaga
      301 tacgaaaaaa atgttctcga aacctgtcgc tcctgtcaaa aatatagcca aatatttttt
      361 tatcaatgta gtaatcagtg ccgcagtctc ggtatttttg caacaaattt tgaaatgggg
      421 tttaacaggc aatccaataa atgaggcttt cagcccgtta ttatttatta ttttgcctat
      481 catgatactt ggagaagaat tgttctcagt gtattttctc gctatttttt ctagtaagtt
      541 cagcattcct gtcgctagtt tcttgtcagc aatcatcttt ggatttatcc attacagtac
      601 ttatgataat ggaaatattt tacacactgt agcacacatc cttttgatac aaggggtggc
      661 aagattgtta ttcaaccaag ctgccattaa aagcaactcg atcattacaa gttgggcagt
      721 tcatgtgata tttgatttca cagctatatt gctagtcgtt ttattctcct aaatttttat
      781 agaaataata catgctctat tgctaagaaa gcatacttta tttcctttaa acaaaaagac
      841 agatgtatca atggat
//
LOCUS       DAHUVV010000234          816 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.242, whole genome shotgun sequence.
ACCESSION   DAHUVV010000234 DAHUVV010000000
VERSION     DAHUVV010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.242"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..427
                     /locus_tag="NK767_002962"
     CDS             <1..427
                     /locus_tag="NK767_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HCH6695866.1"
                     /translation="IYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLL
                     DGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEY
                     QDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(557..>816)
                     /locus_tag="NK767_002963"
     CDS             complement(557..>816)
                     /locus_tag="NK767_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6695867.1"
                     /translation="KYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          309 a           79 c          146 g          282 t
ORIGIN      
        1 tatttataat gatttaactg atatagaaaa agattatata gaaagtttta tggaaagact
       61 aaatgcaaca acagtttttg agggtaaaaa gtgtttatgc cataatgatt ttagttgtaa
      121 tcatctattg ttagatggca ataatagatt aactggaata attgattttg gagattctgg
      181 aattatagat gaatattgtg attttatata cttacttgaa gatagtgaag aagaaatagg
      241 aacaaatttt ggagaagata tattaagaat gtatggaaat atagatattg agaaagcaaa
      301 agaatatcaa gatatagttg aagaatatta tcctattgaa actattgttt atggaattaa
      361 aaatattaaa caggaattta tcgaaaatgg tagaaaagaa atttataaaa ggacttataa
      421 agattgatta tataatatat gaaaagctat tataaaagac attagtatta aatagtttaa
      481 aaaaatgaaa aataataaag gaagtgagtc aattccagac tcctgtgtaa aatgctatac
      541 aatgttttta ccatttctac ttatcaaaat tgatgtattt tcttgaagaa taaatccatt
      601 catcatgtag gtccataaga acggctccaa ttaagcgatt ggctgatgtt tgattgggga
      661 agatgcgaat aatcttttct cttctgcgta cttcttgatt cagtcgttca attagattgg
      721 tactctttag tcgattgtgg gaatttcctt gtacggtata ttgaaaggcg tcttcgaatc
      781 catcatccaa tgatgcgcaa gcttttgaat attttg
//
LOCUS       DAHUVV010000235          801 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.243, whole genome shotgun sequence.
ACCESSION   DAHUVV010000235 DAHUVV010000000
VERSION     DAHUVV010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.243"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..672
                     /locus_tag="NK767_002964"
     CDS             <1..672
                     /locus_tag="NK767_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6695868.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          296 a          125 c          149 g          231 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac c
//
LOCUS       DAHUVV010000236          784 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.244, whole genome shotgun sequence.
ACCESSION   DAHUVV010000236 DAHUVV010000000
VERSION     DAHUVV010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.244"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(479..703)
                     /locus_tag="NK767_002965"
     CDS             complement(479..703)
                     /locus_tag="NK767_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695869.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          282 a          110 c          142 g          250 t
ORIGIN      
        1 ttcttgcaat cttttttgct acgtaatttt tgtaaaaaag tgggttgaga gaaatctaat
       61 tgattttcaa agaaggagta tgaaaatgag aaaaatattt attcgtttat tgtgggtact
      121 atcttagagg aataaataat agtggagaaa actatggttt ggcgcagatt ctatcaggtt
      181 tgatggaacg aagacaacct cccgacatca agtcaaaagc tacttgagat tttgatctga
      241 aaataaaaaa agaaaacaag gtaggaacat caatgtttct accttgtttt cgtaatcaat
      301 gggaaaaaac taagagtggt gtacgtaatc aataaaaagg atggtgacaa cagtacctct
      361 ttactctctg aaagcatcat aactcgtttt tcctcattat gccaataaat tgttgttata
      421 ccacatataa ggatttgtaa tagctaaaaa cagacaggaa agttgaaatc tgtgaatctt
      481 attttgtatg gaaggattta tagaacgcaa aaatcaagat aaagatgaat aaaaatcctg
      541 tttcggctat atattgcgta tctacgccaa taaaacgagt ttgttctgtc atagccatat
      601 aaagcaaagg caaaaataca actacagcga gataagatcc gatgatttct cgaaaagttt
      661 tccaggaagt atgtttcctg tttttatatt tatccgattt cattgaatag tcatagcctc
      721 cttattcttt catatgttga aattccagta taaaaactgg aaggaactgt acgtttttta
      781 gaat
//
LOCUS       DAHUVV010000237          768 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.245, whole genome shotgun sequence.
ACCESSION   DAHUVV010000237 DAHUVV010000000
VERSION     DAHUVV010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.245"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            23..>768
                     /locus_tag="NK767_002966"
     CDS             23..>768
                     /locus_tag="NK767_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HCH6695870.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT          277 a          143 c          133 g          215 t
ORIGIN      
        1 tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc aaactctagt
       61 cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga taaacacact
      121 cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca ctggttttaa
      181 ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat atggtgaatt
      241 aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt taccagccta
      301 caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa aaggtgttac
      361 gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata ctccacaaac
      421 catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat ccagagcctt
      481 aaatgataag tatgtcgcta tttttatgga cgctacttac attccactaa aacgtcaaac
      541 cgtatccaaa gaagcgattt atattgccat tggtatacga gaagacggca ctaaagaagt
      601 actgagttat gcgattgctc caactgaatc aacatacgtt tggaatgagc tgctacagga
      661 tattaactcc agaggagttc aagaagtctt gctttttatt acggacggct taaaaggcat
      721 gaaagatact atccatcaaa tttatcctaa agcaaaatat cagcattg
//
LOCUS       DAHUVV010000238          762 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.246, whole genome shotgun sequence.
ACCESSION   DAHUVV010000238 DAHUVV010000000
VERSION     DAHUVV010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.246"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(109..>762)
                     /locus_tag="NK767_002967"
     CDS             complement(109..>762)
                     /locus_tag="NK767_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HCH6695871.1"
                     /translation="GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPL
                     EAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDV
                     KLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          212 a          175 c          145 g          230 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga cc
//
LOCUS       DAHUVV010000239          737 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.247, whole genome shotgun sequence.
ACCESSION   DAHUVV010000239 DAHUVV010000000
VERSION     DAHUVV010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.247"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(66..465)
                     /locus_tag="NK767_002968"
                     /pseudo
     CDS             complement(66..465)
                     /locus_tag="NK767_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(462..698)
                     /locus_tag="NK767_002969"
     CDS             complement(462..698)
                     /locus_tag="NK767_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCH6695872.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          225 a          132 c          117 g          263 t
ORIGIN      
        1 taaaaaaatc agcatattcc ttaatgacat gctgatctaa ttgagaaata aagtttttaa
       61 ttcgttcatt ttattgcggt ttgtcgtaaa taactggtta tcttggactt tagtgaatcg
      121 aaatccaatg aactagtggt aatcattatg ataaaatcaa cagcttcttg acgagaaaaa
      181 gcaactggac aattattcaa ttgaagaaac aacaacatgg caacaagagc agttcgttta
      241 ttgccattat gaaaaggatg ttttttagct aaattaattg ctaaaatcgc cgctttttca
      301 tagatttcag gatagagttc ttgattgaat actgcttgtg aaggctgttt gacggccata
      361 tctaatgcat ttgcatcttt tacaccgatc atttcgcctg gcgagtgacg gtaaaataac
      421 tttcgcgttg attttgataa tttctttagt cgaaagatac ctcataaatc aaccaaatct
      481 tttaacgcgt cttcatattg attcatgatc gaatcaacta atgaatcaat atcaggttct
      541 tcttgtgctt tgatcattcg tacagaacca tcagattgaa aaacataact gattgcgtca
      601 cctgtctgca cacccatgaa ttcttttact tctggtggga tagttgttac atcgctattg
      661 ccagattttc taattttata ctttttcatt tttttcatat ttcgccaccc tctataccat
      721 tctcattcta tagtaag
//
LOCUS       DAHUVV010000240          717 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.248, whole genome shotgun sequence.
ACCESSION   DAHUVV010000240 DAHUVV010000000
VERSION     DAHUVV010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.248"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..231
                     /locus_tag="NK767_002970"
     CDS             <1..231
                     /locus_tag="NK767_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCH6695873.1"
                     /translation="VPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEK
                     ELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            232..>717
                     /locus_tag="NK767_002971"
     CDS             232..>717
                     /locus_tag="NK767_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCH6695874.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VT"
BASE COUNT          276 a           82 c          129 g          230 t
ORIGIN      
        1 gttcctacaa gaaaatttca attgtttact ttagatgaat gtatcaatgt tgacttatta
       61 aataataaaa taagttttga agaaaacatt gttaaagaaa tgcctgaatc tgctaatgat
      121 atgtatgaga aggagttgaa atatttcttt gaaaacgtag taacaggcaa agaaaactgg
      181 aatgatttat atcatgcaaa cgaagtattg aaaatagctg aggagaaata aatggaaatt
      241 ttatttacta tttgtggtag agctgggtct aaggggttta aaaataaaaa tttgaaatat
      301 ttggcaggaa agcccctagt ccatttcgct ttagcaatga tagatatcta tcaacaaaaa
      361 catcctgaac ataattgcac gacttgtttg aatacggaca gtaatgagtt agtagaattg
      421 acgactaagt taaatccgtc tgtgaaaatt gtagaacgta aacctgaatt agctacagat
      481 actgtaggga aaattgaagt gatacgtgat tcattcttac aactagaaag agaagataat
      541 atatttgatt taattataga tttagatttg acttcacctt tacgtgaagt ttctaatctt
      601 gaagaattaa tagatgaaca tctaaaaaat ttagataaag acgtctactt ttcagttgtt
      661 tctgcaagac gtaatcccta ttttaatatg gtaaaaagaa ttgaaaatga agttact
//
LOCUS       DAHUVV010000241          695 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.249, whole genome shotgun sequence.
ACCESSION   DAHUVV010000241 DAHUVV010000000
VERSION     DAHUVV010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.249"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..675
                     /locus_tag="NK767_002972"
     CDS             <1..675
                     /locus_tag="NK767_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HCH6695875.1"
                     /translation="HFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTT
                     IYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYL
                     NNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKV
                     LLGIPA"
BASE COUNT          241 a          122 c          139 g          193 t
ORIGIN      
        1 cattttaaag gaaagcaatt tcagcaggat gtgattattg tagccgtggg ctactatctt
       61 cgttataacc ttagctatcg tgaagttcaa gaaatcttat atgatcgtgg cattaacgtt
      121 tctcatacga cgatttatcg ttgggtgcaa gaatatggca aactactcta tcaaatttgg
      181 aaaaagaaaa ataaaaaatc cttttattca tggaaaatgg atgaaacgta catcaaaatt
      241 aaaggaaaat ggcattattt gtatcgagcc atcgatgcag atggtttaac cttggatatt
      301 tggttacgta aaaaacggga cacacaagca gcctatgctt ttcttaagcg gttagtgaag
      361 cagtttgatg aaccgaaggt tgtagtcaca gataaagccc cctctattac aagtgccttt
      421 aagaaactaa aagaatacgg cttttatcaa gggacagaac atcgtaccat taaatacctg
      481 aataatttga ttgaacaaga ccatcgtcca gtaaagagac gcaataaatt ctatcgaagt
      541 ttacgcactg cctctaccac gattaaaggc atggaagcca ttcgaggatt atataagaaa
      601 acccgaaaag aaggcactct cttcgggttt tcggtctgta ctgaaatcaa ggtattattg
      661 ggaatcccag cttaaatcat agataccgta aggga
//
LOCUS       DAHUVV010000242          692 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.250, whole genome shotgun sequence.
ACCESSION   DAHUVV010000242 DAHUVV010000000
VERSION     DAHUVV010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.250"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(14..400)
                     /locus_tag="NK767_002973"
     CDS             complement(14..400)
                     /locus_tag="NK767_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695876.1"
                     /translation="MKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRFEVLKIQKNE
                     LLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTDVVMITDIYV
                     EDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          218 a          129 c           81 g          264 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg ttcctttatt tttattaaca agttttatca
       61 caggcttcat atatttgatt atatcttcat cttctacata gatatctgtt atcattacga
      121 catctgtact atttcgattg agtactttca cttgatctcc agcattgatt ttccctctaa
      181 atcttttagg aatgaaccaa ctatacatat gattagcatc accaaaatgt gcttttatcg
      241 cattctcagg atataactta ttcaatagtt cattcttttg aattttcaaa acttcaaatc
      301 tatacttatc atcttctgct aatctaaata aatcttctaa acgcatgttt tctaactgta
      361 agttttgaaa atcttccggt ataaattttc tatatttcat taataatgaa cgcccagccc
      421 gatttattag aatctggcca ttttggtcat ctctataaag aattccgtaa cccatcaacg
      481 ccaatagcac acgtgacgtt ttgttacttg taatattcat atttccttga ttacctgagg
      541 caatgaaaga cttatctact tgaattaggg tgaaattctc attcaaatat ggtagtaaaa
      601 ttaatatatg tgaaaatcga atcttgagat agctattccc cttgagatat tcactgatac
      661 ttctactcaa atcatttaaa tttttataat ta
//
LOCUS       DAHUVV010000243          661 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.251, whole genome shotgun sequence.
ACCESSION   DAHUVV010000243 DAHUVV010000000
VERSION     DAHUVV010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.251"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..>661
                     /locus_tag="NK767_002974"
     CDS             <1..>661
                     /locus_tag="NK767_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCH6695877.1"
                     /translation="ILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQ
                     PGYVQYGAGSYANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTP
                     YNQPGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPV
                     IDPTRAGAANPTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKE
                     LA"
BASE COUNT          227 a          132 c          137 g          165 t
ORIGIN      
        1 atcctattgc atgaaacagc aaacgaaact gcgacaggac gaaatgaagc gcagtacatg
       61 aaacgttctt ggtacaatgc ctatacagcg tatattgttg gcgatggtgg gatcgtttac
      121 caagttggac aacctggtta tgtacaatat ggcgctggtt catatgctaa tgcaaatagt
      181 cctgtgcaaa tcgaactgca acatacgcac gataaagtaa cgtttgaaaa gaactacaag
      241 gcatatgttg aattagcgag agattccgct aataaatacg gtattccact tacattagac
      301 acaccgtata atcaaccagg aatcaaatca catttatggg taactcaaaa catttggggc
      361 gatcacactg atccgtatgg atacttatca gaaatgggtg taagtaagga aaaactggct
      421 tacgatttag ctcatggatt tacagacgaa aacccaacga cttctgagaa caagcctgtc
      481 attgatccaa cccgagcagg tgcagcaaat ccaactttat cagatggaac gaaccattct
      541 cacattgatc agtttggaga aatcgaaaat gcgaacttac acgtcgctgg atggcacatc
      601 gctaactata aatacgagta tattttcatt atggactaca atactgggaa agaattagct
      661 c
//
LOCUS       DAHUVV010000244          642 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.252, whole genome shotgun sequence.
ACCESSION   DAHUVV010000244 DAHUVV010000000
VERSION     DAHUVV010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.252"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            73..>642
                     /locus_tag="NK767_002975"
     CDS             73..>642
                     /locus_tag="NK767_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695878.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFL"
BASE COUNT          230 a           92 c          125 g          195 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccgagaa ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt
      121 ttaaagtgga aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt
      181 atttttactg tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa
      241 ggtcgtttag ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc
      301 ctatatgact atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa
      361 gtaaaagttg gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc
      421 tatacagtag aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga
      481 acagttgtga aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct
      541 attaaaggta cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg
      601 attctatctt tattaattag ttcaataact tttatattct ta
//
LOCUS       DAHUVV010000245          622 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.253, whole genome shotgun sequence.
ACCESSION   DAHUVV010000245 DAHUVV010000000
VERSION     DAHUVV010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.253"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..398)
                     /locus_tag="NK767_002976"
     CDS             complement(<1..398)
                     /locus_tag="NK767_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3'-phosphotransferase/choline
                     kinase family protein"
                     /protein_id="HCH6695879.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECT"
BASE COUNT          203 a           97 c           57 g          265 t
ORIGIN      
        1 gtacattcac taatatctgt ataatctaaa ccgtgcattt gtcttaaaaa actggcaata
       61 tctcgtttta acaaattttg ttcttcttct gacatagtag aataaatttc tggtgttaaa
      121 aaagttcctt taatttcttt ataacctagt atagataatt catcactaat atacgaatat
      181 tcaatattag gaatttttac attagtttct aaatttgtat ttaaaaaatt atatattgct
      241 ttttcttttg cataaccttt tttcttatta gtactaaatt ttgttttaaa aatgtattca
      301 ttattaacta aatatgccac actatcataa ccactaccga ttatttcaat actatctact
      361 ttgaaattat caaagtaatg ctcaattaaa tatttcattg ccttaacatt tgtggcatta
      421 tcatcatatc tatattccat taaataacaa tcttcttttt tgccctcgtg taattcatgt
      481 tctggcaaat cttcaataat tctaaaacca gatttttggt atgcccttat tgctcttgga
      541 ttatttttat gagggtctaa aataactgca ttagcatttc tttctttttt caaaaattca
      601 aaaatcaatt taatatatct tg
//
LOCUS       DAHUVV010000246          618 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.254, whole genome shotgun sequence.
ACCESSION   DAHUVV010000246 DAHUVV010000000
VERSION     DAHUVV010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.254"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..>618
                     /locus_tag="NK767_002977"
     CDS             <1..>618
                     /locus_tag="NK767_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HCH6695880.1"
                     /translation="SGKSLEALELEQEEIRRFQNCSLYPVYHGSAKSNIGIEQLIEVI
                     TNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKIT
                     EMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTV
                     EPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLGKVQM"
BASE COUNT          223 a          100 c          123 g          172 t
ORIGIN      
        1 tccggtaaat cattagaagc attagaactc gaacaagagg aaatcagaag atttcagaat
       61 tgctccttgt accctgttta tcatggaagc gcaaaaagca acatagggat tgagcagctt
      121 atagaagtga taacgaataa attttattca tcaacataca gaaagaagtc tgaactttgc
      181 ggaaatgtct tcaaaattga atattcggaa gaaagacaac gtcttgcata tgtacgcctt
      241 tatggcggaa tcctgcattt gcgggattcg gttagaatat cggaaaagga aaaaataaaa
      301 attacagaaa tgtatacttc aataaatggt gaattatgta aaattgataa ggcttattcc
      361 ggggaaattg ttattttgca aaatgagttt ttgaagctaa atagtgttct tggagataca
      421 aagctattgc cacagagaga gagaattgaa aatccgctcc ctctgctgca aacaactgtt
      481 gaaccgagca aacctcaaca aagggaaatg ttacttgatg cacttttaga aatctccgac
      541 agtgacccgc ttctacaata ttatgtggat tctacgacac atgaaattat actttctttc
      601 ttagggaaag tacaaatg
//
LOCUS       DAHUVV010000247          615 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.255, whole genome shotgun sequence.
ACCESSION   DAHUVV010000247 DAHUVV010000000
VERSION     DAHUVV010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.255"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            136..534
                     /gene="spxA"
                     /locus_tag="NK767_002978"
     CDS             136..534
                     /gene="spxA"
                     /locus_tag="NK767_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCH6695881.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          215 a           93 c          123 g          184 t
ORIGIN      
        1 ttcatttttt taaaaaaatt atgaaaaaaa ggtagctatt taaaagttga ttatgtataa
       61 tagaaacaga tgggagatta ttatgatctt ctgttacaaa aaagtgattc ttatcacgaa
      121 aaggagtggt gcacgttgtt aacattatat acttccccaa gttgtacttc ctgcagaaag
      181 gctcgtgcgt ggttgcaaga acatcagatt ccatttgttg aacgtaatat tttttcagaa
      241 ccattgaaca gttctgaatt aaaagcgatt ttacaaatga cagaagacgg aacagaagaa
      301 atcatctcga cacgttccaa agtattccaa aaactgaaca tggatttaga tgatctgccg
      361 ttacaagaat tattggaact tgtgcaaaac aatcctggat tgcttcgccg tccaatcatg
      421 attgatgata aacgtctgca agttggtttt aatgaagatg aaattcgtcg gttcctaccg
      481 cgcgatgtac gacaattaga attacgtcaa gcacaattga tggctggatt atagaaggat
      541 aatacgaaac ttggaaaaga ctggctcttt gtcaaatgat gttggtaaag agaaatctta
      601 gaataatttg gacag
//
LOCUS       DAHUVV010000248          604 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.256, whole genome shotgun sequence.
ACCESSION   DAHUVV010000248 DAHUVV010000000
VERSION     DAHUVV010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.256"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(318..581)
                     /locus_tag="NK767_002979"
     CDS             complement(318..581)
                     /locus_tag="NK767_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HCH6695882.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          226 a          104 c           79 g          195 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt
       61 ttttcatgtt gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa
      121 cactaaatga aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg
      181 aaataaggga aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta
      241 tttgtttccg atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag
      301 tcgcctttag tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat
      361 gatttggctg tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca
      421 atagaaatga ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct
      481 gcataagaag aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca
      541 aaacctaaaa ttggtaaaac taataataaa ttctttttca taataattcc tctatttttt
      601 tatt
//
LOCUS       DAHUVV010000249          571 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.257, whole genome shotgun sequence.
ACCESSION   DAHUVV010000249 DAHUVV010000000
VERSION     DAHUVV010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.257"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            74..345
                     /locus_tag="NK767_002980"
                     /pseudo
     CDS             74..345
                     /locus_tag="NK767_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          207 a           86 c           99 g          179 t
ORIGIN      
        1 tatgcgtttt tttgttgatt taccaacatg tttagacatt ttcctctagg aaaaaattaa
       61 ctagcaccac gggttaaatc tatattttga tatacaaatt acagcttcag atactaagtt
      121 gcataaacct tttccggacc ccatacaaaa ggcaattgat gaattgggca taaaaaataa
      181 gaaacgattt atattggtga ttcattatat gacatgcaaa gtgcgaaagc ttgtgggata
      241 gcatttggtt tagctaaatg gggagccaaa aatatagaaa attttcaaag tgtagatata
      301 attattgatt ctcctcaaga aatacttgat ttgttagaaa aatagacaaa gagcgtgaga
      361 taaggatagg gtctcacgct ctttgttttt cgccatttaa agattgaaat ttttgccatc
      421 gccgattgta aaattaagct agacaactaa aaaagtcatt tgtgctacac tcaaaatagt
      481 tccgaccaaa gaattataag gagtgagtac aaatgaccta tacccatctt acaacggatg
      541 aacttgtaat aatagagtct tatttcaaaa t
//
LOCUS       DAHUVV010000250          531 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.258, whole genome shotgun sequence.
ACCESSION   DAHUVV010000250 DAHUVV010000000
VERSION     DAHUVV010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.258"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..455)
                     /locus_tag="NK767_002981"
     CDS             complement(<1..455)
                     /locus_tag="NK767_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6695883.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKGIIKS"
BASE COUNT          150 a           87 c           84 g          210 t
ORIGIN      
        1 gatttgataa tcccttttgg ttgtctatga agcttaattc ttactaatga cttcaattta
       61 ggaactttga tgaatttact atcaatcaaa gcaattgtac cattttgatt attcgttgta
      121 taactctgaa caggattttt cttacttttg aaacgtggaa atccaacgga tttatcccga
      181 aaaaaattct tgtatgcttt atctaaatta agttgggcat tggctaaagc aaggctatca
      241 atttctttca aaaacggaaa ttctttcttg tatttagctg gtgtcggaaa agtcattttt
      301 ttagaagaat catttttaac ttcttcatac gccttttttc ggtcatcaag cattagattg
      361 tagaccttac ggacacaacc aaaagatttg gcaaagaaga tttcttgttc ctctgttgga
      421 tagattctga atttgtatgc ttttagttgt tccataaagt tcacctactt tctatttatg
      481 attgtttcct tgattttgga tatacttctt aatcacatca attggtgcgc c
//
LOCUS       DAHUVV010000251          504 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.259, whole genome shotgun sequence.
ACCESSION   DAHUVV010000251 DAHUVV010000000
VERSION     DAHUVV010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.259"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(105..404)
                     /locus_tag="NK767_002982"
     CDS             complement(105..404)
                     /locus_tag="NK767_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCH6695884.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          157 a           97 c           60 g          190 t
ORIGIN      
        1 tttatttaga ataaaaaaat acagactctg ttcaaataaa tgaacctagc ctgcattatg
       61 agattgacta tactttttaa ttcattgaga tgaataactc gtttttataa atgcccaaaa
      121 gtaatcggtg ccgaagccat tcctcgacct aattcttttt caaattttcc tattgttgat
      181 atctccatat aaaacggtga tactggttta gacaattctc ccctgattgc taacggacgt
      241 aaactacgtt ccaaattatt tatgacttta ggataatact gacctttttc taagcctgtc
      301 tttgcgtcta tcaaaagtat acgttccttt tctttgatct ctggatttaa aacgagatca
      361 tataatttta gtaccatgtt ttctatttct tcacttttag acatcacgat ttcctcccaa
      421 aaatatattt cttcccacta cattctataa acaaagtata cgctacatct agattttttt
      481 aaatcattac tggattgtta aaac
//
LOCUS       DAHUVV010000252          502 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.260, whole genome shotgun sequence.
ACCESSION   DAHUVV010000252 DAHUVV010000000
VERSION     DAHUVV010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.260"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="NK767_002983"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="NK767_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCH6695885.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          157 a          110 c           85 g          150 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gt
//
LOCUS       DAHUVV010000253          499 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.261, whole genome shotgun sequence.
ACCESSION   DAHUVV010000253 DAHUVV010000000
VERSION     DAHUVV010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.261"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            85..438
                     /locus_tag="NK767_002984"
     CDS             85..438
                     /locus_tag="NK767_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6695886.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
BASE COUNT          185 a           83 c           87 g          144 t
ORIGIN      
        1 ataaacctat aaaccatata acacgttttt ctatacctgt ttttaattca gtaggaacaa
       61 taaaatgtat agaggtggtc tactatgcgt aaaaaagaag ataaatatga ttttagagcc
      121 tttggtttag ccattaaaga agctcgattg aaacgaggtt taactcgtga acaagtggga
      181 gcattgattg aaattgaccc acggtactta actaatattg aaaataaagg gcaacacccc
      241 agcatacaag ttctttatga ccttgtatcg ttacttcatg tttccgttga tgaatttttc
      301 ttacctgcta ataacttggt aaaaagcacc cgacgattac agatagagaa atacatggat
      361 agctttacag acaaagaact atccttaatg gaatctttag ccagcggtat caacgaagca
      421 agaaacatcg aagactaatt aaaagaatcc atacataacg gaaagagccg ataaaatgag
      481 attgtattaa tctcatttt
//
LOCUS       DAHUVV010000254          495 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.262, whole genome shotgun sequence.
ACCESSION   DAHUVV010000254 DAHUVV010000000
VERSION     DAHUVV010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.262"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
BASE COUNT          165 a           93 c           64 g          173 t
ORIGIN      
        1 taattcgctt gtggcgaaac ccgacctgga gtttgtctct gtattttgaa ccctaaaaat
       61 taatcaaaca gactgatatt ctcataatca gcgcgaaata agccattaag tacagttttc
      121 ctacctattt ttgataaccc gctggttagt taatattcct tttccgagag agataaatga
      181 ttggttaaat ctatatacat ctcaaccttt aaaaatgttt tcgaaatact tcatcagtac
      241 atgtcataca aattaccagt gcattctcaa attctagttc tttaattgat ttcagttgag
      301 gagcagttac ttttggcgga aatttccatt tttgtccaaa cctcttcccc cgatgaaatt
      361 tctttgttgc aattattgca atgaaaaatt tttgtttcaa aaatatacat aattacttcc
      421 tccaataaaa actatctacc tctcaaatta tttttctcta ataagaaaaa cagaaaaggt
      481 aataagatac aacag
//
LOCUS       DAHUVV010000255          465 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.264, whole genome shotgun sequence.
ACCESSION   DAHUVV010000255 DAHUVV010000000
VERSION     DAHUVV010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.264"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
BASE COUNT          151 a           91 c           56 g          167 t
ORIGIN      
        1 cggcgaaagc acaaaaaata aaaaaattat tacgttatta aaaaaacaga tgaaagatca
       61 ggttttatta gatctcattc tttcatcact tgaagggatt taatgaacta ctttttcctc
      121 tttgtcgttt ttatatattc gtattccttc aaacagtagg atagcctctt tcaatgcttg
      181 atatttgaac tgtccgttac ttaaaaattt agatgtgccg ccctcttcct agaacatatt
      241 cttttttact ttttcattaa ttgacagacc aatcgaacga aactgtaaat ttcgatagga
      301 taatgattgg tcaaactcat tcattttcaa gacatctcga ttcatattca agataggaat
      361 ctttttatca acatcacttt attcgtattt tcctctacca taaaagcttc acgctccaca
      421 aaattgtttt atacactact attcagccca ttcaacgacc aatga
//
LOCUS       DAHUVV010000256          452 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.265, whole genome shotgun sequence.
ACCESSION   DAHUVV010000256 DAHUVV010000000
VERSION     DAHUVV010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.265"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            73..234
                     /locus_tag="NK767_002985"
     CDS             73..234
                     /locus_tag="NK767_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCH6695887.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..>452
                     /locus_tag="NK767_002986"
     CDS             253..>452
                     /locus_tag="NK767_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6695888.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNV"
BASE COUNT          173 a           47 c           80 g          152 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gt
//
LOCUS       DAHUVV010000257          451 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.266, whole genome shotgun sequence.
ACCESSION   DAHUVV010000257 DAHUVV010000000
VERSION     DAHUVV010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.266"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(36..272)
                     /locus_tag="NK767_002987"
                     /pseudo
     CDS             complement(36..272)
                     /locus_tag="NK767_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          148 a           75 c           77 g          151 t
ORIGIN      
        1 atagaaggac tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg
       61 atcaaaaaaa tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc
      121 agtgtcttga tatggggaat cttggcctct attttgccat tagaataagg gtaaatcata
      181 gcattgtaaa taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca
      241 tcaagtgttt caggaagatt ttttaataac gccgtttgta taattaagcg agacaaataa
      301 aaaagccatt tatgttacac tccaattgta atacccgcta aagcatacaa ggagagtgaa
      361 cataaatgac ttatacccat cttacaccaa acgagcttgt aatgatagaa gcatattttc
      421 atcaagaaac tccggttgct atcgttgcga a
//
LOCUS       DAHUVV010000258          430 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.267, whole genome shotgun sequence.
ACCESSION   DAHUVV010000258 DAHUVV010000000
VERSION     DAHUVV010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.267"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..>430
                     /locus_tag="NK767_002988"
     CDS             <1..>430
                     /locus_tag="NK767_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCH6695889.1"
                     /translation="QRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIK
                     KLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKT
                     GFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          145 a           81 c           74 g          130 t
ORIGIN      
        1 caaagagcca cgtatagagc ggtttgttcc gtcttatttc ctaatggtga ctttcctagt
       61 aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat tattcgatac
      121 tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag ttttcctagt
      181 ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat agccaaagtt
      241 ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat gatcgttact
      301 aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga cgtgaaagtg
      361 ttagagactt tatcggaaga agcgaatctt cccaatatcc taggggacaa aggatatatt
      421 agccataaaa
//
LOCUS       DAHUVV010000259          411 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.268, whole genome shotgun sequence.
ACCESSION   DAHUVV010000259 DAHUVV010000000
VERSION     DAHUVV010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.268"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..>411
                     /locus_tag="NK767_002989"
     rRNA            <1..>411
                     /locus_tag="NK767_002989"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DAHUVV010000260          399 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.269, whole genome shotgun sequence.
ACCESSION   DAHUVV010000260 DAHUVV010000000
VERSION     DAHUVV010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.269"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
BASE COUNT          111 a           98 c           76 g          114 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttgg
//
LOCUS       DAHUVV010000261          398 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.270, whole genome shotgun sequence.
ACCESSION   DAHUVV010000261 DAHUVV010000000
VERSION     DAHUVV010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.270"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..390
                     /locus_tag="NK767_002990"
     CDS             <1..390
                     /locus_tag="NK767_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6695890.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          156 a           72 c           60 g          110 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattat
//
LOCUS       DAHUVV010000262          394 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.271, whole genome shotgun sequence.
ACCESSION   DAHUVV010000262 DAHUVV010000000
VERSION     DAHUVV010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.271"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..285
                     /locus_tag="NK767_002991"
     CDS             <1..285
                     /locus_tag="NK767_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCH6695891.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          117 a           73 c           92 g          112 t
ORIGIN      
        1 gaggttgcag aagatgatcc ttattgggaa atcgaagcct cctatattgc tcaatgtgcc
       61 tacaatacaa ccttgatgtt ttctccagat gtgatcattt ttggcggtgg tgtgatgaaa
      121 caacgccata tgctgcaaaa cgtccacgac gcatttgcaa aattggtcaa tgggtatgtc
      181 gaaacacctg agttaagcaa atatatcgtg accccagcat tagaagacaa cgctggaacg
      241 ctcggttgtt tggcattggc tcgagaagcg gtcttacatt cataaacgaa aaaaggctct
      301 tcgacattta gtgttggtag agaaagatac aactgaaaat ttagtcataa ccagttaggc
      361 agagtgctta actggttttt gttttacttg gaag
//
LOCUS       DAHUVV010000263          382 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.272, whole genome shotgun sequence.
ACCESSION   DAHUVV010000263 DAHUVV010000000
VERSION     DAHUVV010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.272"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            140..>382
                     /locus_tag="NK767_002992"
     CDS             140..>382
                     /locus_tag="NK767_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCH6695892.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT          138 a           62 c           73 g          109 t
ORIGIN      
        1 attttaaaaa atgataaaaa ttcaaaaaga aaaaatagta gttatttact tgtcatgtta
       61 ctaaataaaa aaataattta aatatgatat atagtcattt atttagtgat ttcattctaa
      121 aataaaggga gaaatgtcta tgctatacgg tgcaattgaa gcaggcggca caaaatttgt
      181 gtgtgccatt ggcgatgaag aaatgacgat caaggaaaga gtgagctttc cgacaacgac
      241 acctgaggaa acaatgcctt tagtcattga cttcttcaaa caataccaag cagatttagc
      301 agggattggg attggctcat ttgggcctat cgatatccat cgcgattccg ccacttatgg
      361 gtacattacc tcgaccccga aa
//
LOCUS       DAHUVV010000264          373 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.273, whole genome shotgun sequence.
ACCESSION   DAHUVV010000264 DAHUVV010000000
VERSION     DAHUVV010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.273"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..>373
                     /locus_tag="NK767_002993"
     CDS             <1..>373
                     /locus_tag="NK767_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCH6695893.1"
                     /translation="FHQETPVAIVAKQLKRGRQTIYNVYNFLKCGGTALEYFEQYKEN
                     KRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFSCSVSTLYRRFKTGEFNV
                     LHLPMQGKRKPNGYKEKRGKQA"
BASE COUNT          131 a           71 c           79 g           92 t
ORIGIN      
        1 attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt ggacgccaaa
       61 caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa tactttgaac
      121 aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct gctgaggaaa
      181 aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt attggccgtg
      241 cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag acgggagaat
      301 tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat aaggaaaaac
      361 gtggaaaaca ggc
//
LOCUS       DAHUVV010000265          365 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.274, whole genome shotgun sequence.
ACCESSION   DAHUVV010000265 DAHUVV010000000
VERSION     DAHUVV010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.274"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="NK767_002994"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="NK767_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HCH6695894.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          120 a           54 c           59 g          132 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atgggacaaa ataaaaaggc
      361 aatac
//
LOCUS       DAHUVV010000266          362 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.275, whole genome shotgun sequence.
ACCESSION   DAHUVV010000266 DAHUVV010000000
VERSION     DAHUVV010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.275"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..>362)
                     /locus_tag="NK767_002995"
     rRNA            complement(<1..>362)
                     /locus_tag="NK767_002995"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DAHUVV010000267          341 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.276, whole genome shotgun sequence.
ACCESSION   DAHUVV010000267 DAHUVV010000000
VERSION     DAHUVV010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.276"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..>341
                     /locus_tag="NK767_002996"
     CDS             <1..>341
                     /locus_tag="NK767_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCH6695895.1"
                     /translation="LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQS
                     VGLYTKTEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRR
                     NGTLFGFSVST"
BASE COUNT          115 a           71 c           70 g           85 t
ORIGIN      
        1 ggttacgaaa gaaacgggat acgcaagcag cctatgcttt cttaaaacga ctccataaac
       61 agtttggtga gccgaaagca attgtgaccg ataaagcacc ttctcttggc tccgccttta
      121 gaaagttaca gagtgtgggt ttatatacta agacagagca ccgaactgtg aagtatctta
      181 acaatttaat agaacaagac catcgaccta ttaaacgacg aaataaattt tatcaaagtc
      241 tccgtacagc ctcttccacg attaagggca tggagaccat tcgaggaata tataaaaaga
      301 accgaagaaa tggaacgctc ttcggctttt cggtgtctac t
//
LOCUS       DAHUVV010000268          334 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.277, whole genome shotgun sequence.
ACCESSION   DAHUVV010000268 DAHUVV010000000
VERSION     DAHUVV010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.277"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..>334
                     /locus_tag="NK767_002997"
     CDS             <1..>334
                     /locus_tag="NK767_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCH6695896.1"
                     /translation="GKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIV
                     GLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKE
                     FSNWKQISN"
BASE COUNT          124 a           55 c           65 g           90 t
ORIGIN      
        1 ggcaagcgta aaccgaatgg acatcaagaa agacgtggaa aacaaacttt ccgccgctct
       61 attcatgaac gtgaaaagga ttatagccaa ttctcaaatg agtttggtca ccttgaaggt
      121 gacactatcg taggtctgaa acataaaagt gctgtaatta ccttagttga acgattatca
      181 aaagttatca tcacattgaa accgtgtggt agacaagcga ttgatattga aaaaaaatta
      241 aatcaatggt ttgaatctgt accgaaaaac ctattcaaat ccatcacttt tgattgtgga
      301 aaggaatttt caaattggaa acagatcagt aatg
//
LOCUS       DAHUVV010000269          319 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.278, whole genome shotgun sequence.
ACCESSION   DAHUVV010000269 DAHUVV010000000
VERSION     DAHUVV010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.278"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(<1..>319)
                     /locus_tag="NK767_002998"
     CDS             complement(<1..>319)
                     /locus_tag="NK767_002998"
                     /inference="COORDINATES: protein motif:HMM:NF025072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCH6695897.1"
                     /translation="KGIGVLDSFFEKFGIEDAWTFAGDYSDSKKFYEKYEDKVQFVGH
                     VSKIKMFELYRDNDVLVFPSLADGFGLAVIEALSNGMYVICSDSTGAKDIIMEGKNGD
                     VFLT"
BASE COUNT          115 a           72 c           25 g          107 t
ORIGIN      
        1 tgtaagaaac acatctccat ttttgccttc cataataata tctttagcac ctgtagaatc
       61 tgaacatata acatacattc cattgcttaa tgcttcaata acagctaaac caaaaccatc
      121 cgctaaagaa ggaaatacta aaacatcatt atccctgtac agttcaaaca tttttatttt
      181 tgatacatgt cccacaaatt gtaccttatc ttcatatttt tcataaaatt ttttagaatc
      241 tgaataatcg ccagcaaatg tccatgcatc ctcaattcca aatttttcaa aaaaactatc
      301 taaaacccca attcctttt
//
LOCUS       DAHUVV010000270          308 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.279, whole genome shotgun sequence.
ACCESSION   DAHUVV010000270 DAHUVV010000000
VERSION     DAHUVV010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.279"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            55..>308
                     /locus_tag="NK767_002999"
     CDS             55..>308
                     /locus_tag="NK767_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HCH6695898.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQ"
BASE COUNT          124 a           44 c           75 g           65 t
ORIGIN      
        1 gagcttatgg attgattcaa aaggacgaca gtttaggtat gtggaggaga gataatggat
       61 ctcattacac aatacagtga catcatcctc aagaaaatca tgatgaagat tcagaaagac
      121 aaaaaatcaa aagaacgagc tgaattagtt aagttagaaa tggctgaaac aggagcagga
      181 gtgcgaagta gcaggcattg gaaagcagca gcaaacattg aattttatta caacgaaatt
      241 caaaaagggt tcgatcagat gcgtgagctg gatcggcaaa caaattggag caagaaactt
      301 catcaaga
//
LOCUS       DAHUVV010000271          303 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.280, whole genome shotgun sequence.
ACCESSION   DAHUVV010000271 DAHUVV010000000
VERSION     DAHUVV010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.280"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(53..>303)
                     /locus_tag="NK767_003000"
     CDS             complement(53..>303)
                     /locus_tag="NK767_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6695899.1"
                     /translation="FVKDFYKVWDSEVFFLAIEDSLLINLYYSNKNYFKIPATKTRMK
                     KDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT           97 a           59 c           46 g          101 t
ORIGIN      
        1 ataagtatgg tgataaaatc cgttccgggc tacctttttt aggtagccta ctttaatctt
       61 tatacctttc tggatcaacg aaagtatact tcaaatagtc ataacgccga tgatcgctcc
      121 gtgcgtctgg cacatcagtc acgatatcaa acaaaaaata cacatctttc ttcattctgg
      181 tttttgttgc cggaatttta aagtagttct tattagagta atagagattg attaataagc
      241 tatcttcgat tgctaaaaag aaaacttctg aatcccacac cttataaaaa tctttgacaa
      301 atc
//
LOCUS       DAHUVV010000272          303 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.281, whole genome shotgun sequence.
ACCESSION   DAHUVV010000272 DAHUVV010000000
VERSION     DAHUVV010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.281"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
BASE COUNT          111 a           36 c           57 g           99 t
ORIGIN      
        1 taatcttctt tatagagaat tgggctattt ttaatggcaa agaggggaag aagatgtttc
       61 aaaacaaagg acaacgctat gttgtaaaag gagtaaagga atcttactgg aatcaaaaat
      121 ctttattgag aaaattatct ttgaatttaa gcacaaagaa atgaaggtac actagatagc
      181 aatgaagata tctttgtcat taaacgtcgc tattttacac aagtaagtga tgatttgatt
      241 catgtctttt ccaatgagtg gtttgtttct gagaaaaaat tacatgcttc taatctgcaa
      301 tat
//
LOCUS       DAHUVV010000273          287 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.282, whole genome shotgun sequence.
ACCESSION   DAHUVV010000273 DAHUVV010000000
VERSION     DAHUVV010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.282"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(23..268)
                     /locus_tag="NK767_003001"
     CDS             complement(23..268)
                     /locus_tag="NK767_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCH6695900.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT           79 a           63 c           53 g           92 t
ORIGIN      
        1 gaatctcagg ctcgtttgct ttttaaactg cttcagttaa aaattcgatc tcaactgtcc
       61 gagtaagtac atctgaatag ctgtaagata ctcgttcaaa agaattttct tcaggatcca
      121 aatcaacaac gaatactgaa ggataggttt ctgttaaaac gccgcggcgt tctgtttgac
      181 gctttcttcc tgtctgagca accaacatga ttgggctgcc gatacgacct tctaaatctt
      241 tcttgattga agctaaagtt gttggcatac agttcacctc tactatc
//
LOCUS       DAHUVV010000274          279 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.283, whole genome shotgun sequence.
ACCESSION   DAHUVV010000274 DAHUVV010000000
VERSION     DAHUVV010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.283"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            1..279
                     /locus_tag="NK767_003002"
                     /pseudo
     CDS             1..279
                     /locus_tag="NK767_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          107 a           56 c           55 g           61 t
ORIGIN      
        1 caaatcgtcg atctctatct cgctggtaag cctcgcgcag aaattattcg agagtatgag
       61 cttacgcctt cttctttcga taaatggatg aagcaagcac aatcaacggg ctcattcaaa
      121 gaaagagaca acttaacacc agaacaagca gaattgatcg cactaagaaa gaaaaataag
      181 caactcgaaa tggagaatga tattttaaag caagcggcgc tgatattcgg acgaaaagac
      241 aagtaattga tgccaacaag cataaatatt ccatatcag
//
LOCUS       DAHUVV010000275          270 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.denovo.284, whole genome shotgun sequence.
ACCESSION   DAHUVV010000275 DAHUVV010000000
VERSION     DAHUVV010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.denovo.284"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..>270
                     /locus_tag="NK767_003003"
     CDS             <1..>270
                     /locus_tag="NK767_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCH6695901.1"
                     /translation="MKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVG
                     GGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMA"
BASE COUNT           65 a           55 c           77 g           73 t
ORIGIN      
        1 catgaaaaaa gaatttccga tcccgatttc ttggacaaca gatgtcaatg cggctgccta
       61 tggtgagtat gtcttcggca gtggcaaagg attgagcagt gtcgtttact atacgatcgg
      121 tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt atcgaagggt ttagtcatcc
      181 agaaatgggg catatgctcg tcgtccctca tccaaaagat gactttgctg gcagctgtcc
      241 atttcatggc aactgtttag aagggatggc
//
LOCUS       DAHUVV010000276        20043 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.guided.047, whole genome shotgun sequence.
ACCESSION   DAHUVV010000276 DAHUVV010000000
VERSION     DAHUVV010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.guided.047"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(695..1630)
                     /gene="whiA"
                     /locus_tag="NK767_003004"
     CDS             complement(695..1630)
                     /gene="whiA"
                     /locus_tag="NK767_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCH6695902.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(1664..2662)
                     /locus_tag="NK767_003005"
     CDS             complement(1664..2662)
                     /locus_tag="NK767_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCH6695903.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(2659..3543)
                     /gene="rapZ"
                     /locus_tag="NK767_003006"
     CDS             complement(2659..3543)
                     /gene="rapZ"
                     /locus_tag="NK767_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCH6695904.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(3678..4409)
                     /locus_tag="NK767_003007"
     CDS             complement(3678..4409)
                     /locus_tag="NK767_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HCH6695905.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(4413..5678)
                     /locus_tag="NK767_003008"
     CDS             complement(4413..5678)
                     /locus_tag="NK767_003008"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HCH6695906.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(5941..8757)
                     /gene="uvrA"
                     /locus_tag="NK767_003009"
     CDS             complement(5941..8757)
                     /gene="uvrA"
                     /locus_tag="NK767_003009"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCH6695907.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(8767..10761)
                     /gene="uvrB"
                     /locus_tag="NK767_003010"
     CDS             complement(8767..10761)
                     /gene="uvrB"
                     /locus_tag="NK767_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCH6695908.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            11020..12522
                     /locus_tag="NK767_003011"
     CDS             11020..12522
                     /locus_tag="NK767_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HCH6695909.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            12524..13261
                     /locus_tag="NK767_003012"
     CDS             12524..13261
                     /locus_tag="NK767_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCH6695910.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(13432..14625)
                     /locus_tag="NK767_003013"
     CDS             complement(13432..14625)
                     /locus_tag="NK767_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6695911.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(14704..14910)
                     /locus_tag="NK767_003014"
     CDS             complement(14704..14910)
                     /locus_tag="NK767_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCH6695912.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(14903..16045)
                     /locus_tag="NK767_003015"
     CDS             complement(14903..16045)
                     /locus_tag="NK767_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCH6695913.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            16557..17192
                     /locus_tag="NK767_003016"
     CDS             16557..17192
                     /locus_tag="NK767_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6695914.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            17346..17423
                     /locus_tag="NK767_003017"
                     /pseudo
     CDS             17346..17423
                     /locus_tag="NK767_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            17668..17784
                     /locus_tag="NK767_003018"
     CDS             17668..17784
                     /locus_tag="NK767_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCH6695915.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            17800..19719
                     /gene="tet(M)"
                     /locus_tag="NK767_003019"
     CDS             17800..19719
                     /gene="tet(M)"
                     /locus_tag="NK767_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCH6695916.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            19838..20005
                     /locus_tag="NK767_003020"
     CDS             19838..20005
                     /locus_tag="NK767_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCH6695917.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
BASE COUNT         5858 a         4082 c         3637 g         6466 t
ORIGIN      
        1 ataaatgtta tgagacctgc tcgaagtaaa acccgatata ataaaaatgc tgtatttcgc
       61 tttgctatat ctagaaaaat aaaaagtact gcaatcataa tgatagtaaa agttgtaaaa
      121 aagcacattt tttgccctcc ctttataaac atcgattctc aaattctaaa ccaaatcttg
      181 atagttcaag ttcaggtata acgtatacca accatacatc ccaattacta tttatgaact
      241 tatgattatc actgaataaa tcactccatc cccttgttaa aacttctaca ttaattacac
      301 ctggttgttt tcgactagct aatccaatga acttatcaac catccttatc caccaaccac
      361 gagaatcttg accagaagta agaaaaacag ttctcaaggt tgatcgagca gttccttcca
      421 gtttttaatt aatctccagt tatagataaa tacttgatcc tatctatatc aggacttcta
      481 tattaagcaa aagtttatgt ataaaactaa aatcttcatt cagttgtctc cccgcttttt
      541 atacgattgt attaaaaaac aatagaagcc tttcaacatt atgttactcc agtaaagttg
      601 gaattatagt ttgacggaag aaaagcaagt atagctttta aaaaaaactg atacattcgc
      661 atctcctgct tcatgtatca gttttttctt tggattaaac tgcttcttca cgcagttttt
      721 ccgcaaattc atttattttg cgaattcggt gattaattcc cgattttgat attgcacctg
      781 agggaatcat ttcccctaat tctttcaagc tgacctctgg atgttgaagc cgaagttctg
      841 cgatttcctg aagtttttca ggaagggcat gtagaccgac ctttgcttcg atcaattcga
      901 tgttttcgat ttgtttagaa gccgcatcaa tcgtcttgtt cagatttgct gtctcacaat
      961 tgactagacg attgaccgaa tttctcatat cccgtacgat tcgaacatct tcaaatcgga
     1021 gcattgagtt ggttgcacca atcaatgtta agaaatcagc aattttttct gctcctttta
     1081 aatacgaaat atagccattg cgccgttcaa gcgttcgagc atgtagaccg taataattta
     1141 acatatcgca aatgtcttgg ttatgctgtt cataaataga aaatatttcc aaatgataac
     1201 gactcgtttc cggattattg actgaacctg aggccataaa agcaccacgt aagtaagaac
     1261 gcattttttg cgaatttccc ataatctcgt ccgaaacgtg tccattgaac atcataccat
     1321 ccatgatatc aagatccatc aacacatctt ttgtgccttg cttcaaccgg acgatataga
     1381 cattgttctt ttttaatttc atttttttac gaacgagtag ctcacttcgt acctgataat
     1441 gatctttcaa aagagaatag atacgtctgg caattgcagc attttctgtt tggacgttca
     1501 aaataaactg ctggttcaca atacctaaag agccgttcat tcgcagcaaa gcagctaatt
     1561 ccgctttcgc atgttctcga tgaacttcca aaccagttag ttctttcttc acatctgcag
     1621 caaatgacat cctattctcc tcctttctcc aacaataaat tgcttaatat tttgaaccga
     1681 aaactaaacg gaacaattct tctactactt tgtctccatc gtggaagaca ccaccatctt
     1741 tcaattctaa aaagtcggtg gaaatcactc gacatccttc atttctcaat ccgttgaagt
     1801 cgtgtgcgac ttgtactaga tactcatcat agatctctgg gtccatataa tcttcaggca
     1861 ctttttccgt attgaccaat actgtatcga taaaaggctg attcaaatgc ttgtgtaaaa
     1921 cacgtatatg atcggcatct gtgaaatgtt ccgtttcccc tttttgggtc atgatattac
     1981 agatatagac ggtttcagca gttgtcttga gcattgcttc gccgatttct ggtatcacta
     2041 aatttggaag gatactggtg aataaacttc cgggtcccaa aacgaccatg tcggcgtcca
     2101 taattgctga aacgactttt cgagccgctt tggcttcact tttatcattt gtgttgcaga
     2161 catacacacg gtcaatggtt ttacggttga tcgcgatttt agattctcct actgcagtcg
     2221 ttccgtcttt aaaaacagca tgcaaggtca aaggagtttc tgaagaagga taaacatgtc
     2281 catctacatg catcattttt gctaataatt gaatcgcctc ataagtgctt ccccgcatct
     2341 cggaaactgc ggcaatgatc agattaccga tcgtatgatt ggctaaaaac tgatcttcct
     2401 ttttgaaacg gtattggaaa atatcttcat agaattgtgg catatccgat aacgccacta
     2461 agacatttct caagtcacca ggaggagcca tagcgatcga ttctctgatt gcaccgctgc
     2521 ttccaccatc atctgcaaca gtcactactg ctgtgatatc tgctccttga tttcgtaaac
     2581 ttttcaggat aactggcaga ccagtgccac cgccgaccac tacgatcttt ggttttctta
     2641 tccggtaggt tttcattttc acgaccggtt caccgtctct ttccttttat ccttgtcccg
     2701 atgtgtcaca ttgactttat agtttttccc taaagcattt gccaaacgtt cagtcaatgc
     2761 cacagaacgg tgttgaccgc ctgtacagcc gattgcaact gtcaagctgc tttttccttc
     2821 tttaatgtat ccaggcaata cagtttccag aagatccaaa aatttatggt agaatgattg
     2881 cgtttcatca aaggacatca cataatcata gactggctga tcttttccgg tcaatggtct
     2941 tagttccggg atataatgag ggttgggcaa aaaacgcaca tccatcacaa tatccgaatc
     3001 gatcggcaac ccgtatttaa aaccaaatga tacaaattcg atccgaaatc cttggtctcc
     3061 ctttgatttg aatgactcat tgattttttc acgtaattgt cgtggtgtca atgttgtcgt
     3121 atcaatgatc acggaagctt gggctttcaa gtcgtctagt atcgcccgtt ctttacgtat
     3181 cccttcagtg atcaaaccgt ccatcgccat tggatgtgct cgtcttgttt ctttgtagcg
     3241 agccactagt tcttcgtctg aagcatctaa aaataagata gaagtgtcga tgaagttcgt
     3301 attttcgatt tcgatcagca tgttttggat ttcttcaaag aaggaacgag aacgcaaatc
     3361 cactcctaaa gccatttttg tgactttacc tgattctttg atcaattccc agaattttgg
     3421 aatcaatcga ggcggcatat tatcgataca aaaatagccc atatcttcaa aactttggat
     3481 agctactgtt ttccctgcac cactcatacc tgttatgatg actaattgca aattatctgt
     3541 catatttacg aacccctctc acattgtgca taataaaatt atatcatccc gggcgtatca
     3601 ttaccagttt tctttttctt ttacaaaaag atttggacca aatatctttt aggatatttg
     3661 ccccaaatcc aattttttta cttttccgat gctgtatcca atgcctcgct tctttcagct
     3721 aatgcacgtt cgatcgttcg atccgctaaa atagattgag catcgatcac tgggtaatga
     3781 ttatcttcag caaattcatg catcaaagat aattctgtgc aacctaggat gactttttca
     3841 cagtttaggc gctctaccgc ttcctctaag atttcgtaat agagctcttg attcaaataa
     3901 tccgattcct tgatctcatg atagatcagg taattgattt tttcttgaag cgcagtatca
     3961 ggaatcactg tttcgaaacc aagatttttg acttcgcgtt catagattcc agctttcata
     4021 cttccttctg ttcctagaat cgctactctc cctgtcgtat gctgacggac aagttcattt
     4081 gctgcttctc ttggcatatg caagatagga atatccgttg ccgcttgtaa ctcttcaaaa
     4141 aagtagtgtg cggtgttaca agtcaacaca atgaagtttg gtcgcaataa attttgtttc
     4201 tcaatatcat ccagcagaaa cggcattgga ttttcttctg atcgatctaa aatataagct
     4261 gtacgatcag gaactgttgc atgattgaat aagacataat tcaaatattc ttgatctttt
     4321 gtagctttcg ttcggtggtt gatcaagcga acaaaacttt ctgtcgccat cgtgcccatt
     4381 ccgcctaaaa tactgaaaaa attctccatg aactagcctt ctttcacatg aaaatacttt
     4441 ttgaatcttg gaatatagtt atggaacata tatttcatca acaaccagcg cttgattgat
     4501 cggtcttttt cataaaatac agtagtaccg taacgatttt ctttgatcat ttgctcagct
     4561 agttttttgt cttcattttc ccgagcatat tctaaaaaga tttttttggg cacacccatc
     4621 cataatcttg ctttatctga ttgattcgtt ccataggtcg tttcaacaaa gggtttgttg
     4681 aacactcgat cttctgtgac aaaacgagcc agattcaagc cgttcaatgt cacaaagaaa
     4741 ctgctacgac cttgtctcaa attgatttca aataatttat attctccatc tctaggatca
     4801 tacttcatat cgaaattggc aaagcctgta tattcgattt tttccaaaaa tgccttaatg
     4861 gtttgataga ttttttcgtt ataatctggc atgatcacaa cgtaattacc aatcgatgct
     4921 ggtgtaggat cttctaatag tggatgacca aggcacatca tccgaacttg atgatcttca
     4981 tctacatacg catttagcac acgcatatta ctatcatcac ccgggataaa atcttgtaca
     5041 atcatttcac ttttataacc ggcttcgtat atccggccta aaattaaatc gaactcttct
     5101 ctagtctcta aaataaaagc tttttttctt ccttcaaatt ggacagaaag atactccaca
     5161 ctgtttgcag gtttcaaagc aactgggaat tcaaaaggca attcctgttc caaatgtcca
     5221 tttactaaca tttcttctct gacgatcaat gttttaggat acggaagatc atattcttca
     5281 caaacttcat aaaagcttac tttattgatc agcctttcaa ataaagaata atcaatatat
     5341 ggacagataa aatattctga cagctcttgt ttatgttggg agatcaattc tgcatatcca
     5401 tcaccacaag caatcaaaag atatttttta ctcttatcag aatatctttc ttttgccaaa
     5461 cgaagcatcg tctcgataaa tacagggtct ttatcaaatc ctggaataac ttctacattg
     5521 acaattttac tgtagcgggt aggggctaac tgatccgaag catatgcttg gcagattttg
     5581 ccgtatgctt cattaaaaga acgtgccata ccataaacgt tcatgtcgct tcctaataat
     5641 atagggataa attcttcatt ttcaatactg ttcatcatta tctctcactt actcataatc
     5701 tcttgttttt cttttccaac tacacaagga atatcatacc ataaagaaaa aagcaatcaa
     5761 aggaaagtcc tccgattgct caattctttt ttgaacggat tgaacttata ggaagttttt
     5821 atctatacca atcagtgtag aaagaactac catgtaatga ttctagaaca ctgatattaa
     5881 taaaacaagc aaaaattcat ttttttatgt ttttttaact atctcagcta tctatcctct
     5941 ttataccatt atttttttca aatagtgacc tgtataactt tcttttacgt taatgatttc
     6001 ttctggtgtt ccagtcgcaa ggatcgttcc tccaccttct ccaccttctg gtcctaaatc
     6061 aatgatataa tctgctgttt tgattacatc tagattgtgt tcaatcacta aaactgtgtt
     6121 accagcatct actaatcttt gtaatacatg caacaagcgg gcgatgtcat cgctatgaag
     6181 tcctgtcgtt ggttcatcta gtatatagaa attctttcca ttagagattt tgtgcaattc
     6241 actggcaagt ttcatccgtt gtgcctcacc accggacaat gtcgttgctg gttgccccat
     6301 cgtcacataa cctaagccaa catcaacaat cgtttgcagt ttgcgatgaa tctttggaat
     6361 gtgcttgaag aattctacag catcctctac tgtcatttcc aaaatatcag caatgctttt
     6421 tcctttgtaa tgcacttcta atgtttcaga attatatcgt ttaccatgac atacttcaca
     6481 aggaacatag acatcaggca aaaagtgcat ttcgatctta ataattccat ccccgcgaca
     6541 agcttcacaa cgaccgcctt ttacgttgaa actaaaacgc cctttcttat aaccccgcat
     6601 ttttgcctcg ttcgtttgag caaataaccc acggatatca tcaaatacac ttgtataagt
     6661 cgctggatta cttctcggcg tccggccgat tgggctttga tcgatatcga tgatcttttc
     6721 gatactttcg tagccggaaa ttgtcttgaa tttacctggc ttagcagaat tcttatttaa
     6781 tttttgcgct aacgatttct ttaagatact attgaccaat gtactctttc cagaaccaga
     6841 cacacctgtg actgctacaa attctcctaa tggaaattca acactaatat ttttcagatt
     6901 gttttctgaa gcaccagtga tcttgattgc tttcccatta cctttacggc gttcttttgg
     6961 tacagggatt gattttttcc ctgaaagata tttccctgtc aaagaatttt catcattagc
     7021 tacttcttca ggagtgcctg cagccacgat ttgtccgcct tgttctccgg ccccaggtcc
     7081 gacgtcaatc aaataatctg ctgcccacat cgtatcttca tcatgttcga ctacaacgag
     7141 ggtgttgcca agatcacgca ttttctttaa tgagccaatc agtcgatcgt tgtcgcgttg
     7201 atgcaatccg atcgatggtt catctaaaat atacaaaact ccagaaagat tcgatccaat
     7261 ttgtgtagcc aatctgattc gctgcgcttc tcccccagat aatgttccgg aagcgcgact
     7321 caaagttaga tagtctagtc ccacattttc caagaatgaa agtctgtcat ttacttcttt
     7381 taaaatcggt ttggcaatta ctttctcttg ttcagataac gttaagtttg agaagaaatc
     7441 tactgctttt tgaatagaca actcatttgt ttgcccgata ttcgttccat tgatttgaac
     7501 agatagagct tggggattca atcgataacc tttacatgtc tgacaagtta attctgtcat
     7561 ataaagacgc atttgctcac gcgtgaaatc actgttcgta tcatgataac gacgctcgat
     7621 attcttcaat accccttcga aagtcgtttc tacatcgcga acgccaccaa aatcattttc
     7681 ataatggaaa tggaaaagat catctgtccc attcaagatg atctcctgat gctcttccgg
     7741 taattcttcg aatggtgtat ctaaatcgat accgaaactt tcacaagcct gcgctaacat
     7801 ttgaggataa tactgagagc tgattggatt ccatggaacg atagcacctt gactaagtgt
     7861 cttcgttcta tctggaatca ctaaatcttg atcgacttct aatttgacac ctaaaccatc
     7921 acagtctgga caagctccaa aaggcgcatt gaatgagaac agtcttggtt ctagttcgcc
     7981 aacagtaaat ccgcaataag gacaggcata gtgttcgcta aacaacattt cttcttcacc
     8041 gatcacgtca accagagcat agccgtctgc taatcgcagg gccgcctcta aagaatcaaa
     8101 taggcgagaa cgaatccctt ctttcacaat gatccggtcg atgacgatcg cgatatcatg
     8161 ttttttgttt ttctccagct caggcacttc acttaaatca tagatctcac catcgactcg
     8221 aacgcggacg tatccctctt tttggattcg ttcgaatatt tttttatgtt gtcctttttt
     8281 ctttaccacg atcggtgcca acacttggat ctttgtgcgc tcaggcaaag ccattacttg
     8341 atctgccatt tgatctactg attgactggt aatctcgata tgatcgttgg gacaaatcgg
     8401 atgtcctaca cgtgcaaaca acaagcgtaa atagtcattg atttccgtca cagtacctac
     8461 tgtcgaccga ggatttttac ttgttgtttt ctgatcaata gaaatcgccg gactcaatcc
     8521 gtcgatgcta tctacgtccg gcttatccat ttgtcctaaa aactgacgag cataagcaga
     8581 aaggctctct acgtatctcc gctgcccttc agcgtacagc gtatcaaatg ctagggaact
     8641 ttttccagaa ccggacaaac ctgtaacgac cacgaattta tctcgtggaa tcgttacatc
     8701 tatatttttc aagttatggg cacgtgcacc atgaatcgta attttatcgt tagccatgtt
     8761 gtcctcctac tttgaagctt ttaattctaa aatcatatct cgcacattcg cggctgtttc
     8821 aaaatccaaa gcttttgccg catctttcat ttctttttct aatttcatca ataacgtgtc
     8881 tttttcttcc ttcgtcattt cttcgtacga gacttgtacc gcttcttttg tatcatcttc
     8941 cgattctttc gtaatagaaa tcaaatctcg gatttcttta atgatcgttt ttggaacgat
     9001 cccatgttct tcattgtact tctgctgaat cgtacgacgt cttgacgttt catccatcgc
     9061 tagacgcata gaatctgtta ctttgtctgc gtacatgatt actttacctt cttcattccg
     9121 agcagctcgt ccaatcgttt ggactaagga acgttcacta cgtaaaaagc cttctttatc
     9181 cgcatccaag atagcaacaa gggaaacttc aggtacgtct aatccttcac gaagcaaatt
     9241 gattccaact aacacatcaa attcacctaa tcgaagatct cgaatgattt ctgttcgttc
     9301 cagcgttttg atatcactat gcaaatattt gactttgatc cctaattctt tgaaataatc
     9361 tgtgaggtct tctgccattt ttttcgtcaa tgtcgttaca aacacccgct ggtctttttc
     9421 gactcgttca tttatttcgc ctactagatc atcgatttgt cccatgattg gacgaacttc
     9481 gatcactggg tcaagcagcc ctgtcggacg gatgatttgt tggataacgg tatctgtttg
     9541 ttcatgttca tatggtcctg gcgtcgcaga gacataaatg atctgattga catgtttttc
     9601 aaactcttct aaccgaagtg gccgattgtc taaagcagat ggcaaacgga aaccgtaatc
     9661 tactagcatc tgttttctcg ctcggtcacc attatacatt cctctgattt gaggcatggt
     9721 tacgtgcgat tcgtccgcca cgatcaaaaa atcttcagga aagaaatcca atagcgtata
     9781 aggcggttca ccttctttac gtccgtccat atgccgagaa taattttcaa ttcccgacgt
     9841 gtagcccatt tctaacatca tttcaatatc gtaattggtt cgctgttcta aacgttgagc
     9901 ttccagaagt ttgttttcat ttcgcaagac agttaaacgc tgttctaatt cctctttgat
     9961 attcgctacg gcatgctcca tatgttcatc attcgtcaca aagtgagtcg ccgggaagat
    10021 tgctacgtgt tccgtttctc ctaaaacttc acctgtcagt gcattgactt ctcggatacg
    10081 ttcgacctca tctccaaaaa actccacacg taaggcacgt tcatctcttg aagccggaaa
    10141 aatctccacg acatcaccgc gcactcgaaa acgaccgcgc tgaaaatcta tatcatttct
    10201 ttcgaattgg atactgacta agtcacgaat caactgattg cgatctagtt ctgctccctg
    10261 tcgaatcgaa actacttgtt tttggtactc aaaaggcgag cctaatccaa aaatacatga
    10321 aacggatgca atgacgatca catcatttct ttctaataat gaactagtag ccgaatgtcg
    10381 taatttgtcg atctcatcat ttacacttga atctttttcg atatacgtat cgcttgaagg
    10441 cacataagct tcaggctgat aataatcata ataagaaaca aaatattcaa cagcattgtt
    10501 agggaaaaat tccttgaatt ccccgtacag ctgaccagcc aatgttttgt tatgggcaat
    10561 aatcagtgtt ggtttattca ctttctcaat aagattggaa atcgtatatg ttttccctgt
    10621 tccagtcgca cctaataaga tttgtgcttt tttcccgccg actacgccat ccactaattg
    10681 gttaatcgct tcaggctgat caccagccgg ttggtatttc gagacaagct caaagtgtcg
    10741 ggaggtatct ctctcaatca ttcttcgtcc tccttttcat tcattccttt tcattttacg
    10801 accaataaaa agaaaaggaa agcaaaggca ttccttacta aaaaattata ccacaccgaa
    10861 catactttcg ccactttttt atcttattat acatgttatg ttttataaca taaaatgtta
    10921 gaaactcgca tttttttcat tttttcgagc ttacagtctt gtctgattca gcacttttcg
    10981 cctataataa taaaatattg gaaatttagg aggttgatta tgaaaagaaa gcatttattt
    11041 cactcatttg ttttcttaat gacaggactt gtgacacttg cgtttggtca tgttgcacaa
    11101 gctgacaaca ctacaacggc agagtcaagc acaagcgaga caatgcaaaa aatagagccc
    11161 aaaaaagacg tatacgtaat tgccagtgac tctgcttttg ctccatttga atttcaaaac
    11221 gcaaagggcg agtacgaagg catcgatgta aaattgatgg aaaacatcgc caaactgcaa
    11281 ggatttaaca tccgaatgga ctaccgtggt ttctctgctg ctttgcaagc tttggaatca
    11341 ggacaagcag acggaatgat tgccggaatg actgtaacag atgaacgaaa gaaaagctat
    11401 gatttttctg attcttactt tgatagcggg atccaattag ctgtcaaaaa aggagatact
    11461 tcgatcaagt cttatgaaga tttgaaagga aaaacagtcg gagcaaaagt tggtaccgaa
    11521 agtgctgaat ttttaagcga ccatgaatct gaatatggct attctatcaa atattttgat
    11581 gctgctgatc aattgtacga cgggttaaaa gttggcgcaa tcgatgcaat gatggatgat
    11641 tatccggtaa tcggttatgc gatcaaacaa ggacaagaat tggaaacacc gatcaaaaaa
    11701 gaatctggcg gaaaatatgc atttgccgtt aaaaaaggac aaaatcctga gcttcttaat
    11761 atgttcaatg aaggattgaa agaattaaaa agaacaggcg aatacgataa gattattaaa
    11821 gaatatgtag cggatggttc agaaacttcc agtgagtctt ccactgctga tgaatcaaca
    11881 tttcttggac ttatccaaaa caactatcaa gcattgttga aaggactttg gagaacaatt
    11941 ctcctaactt tgatctcctt tgctttggct atagtaatcg gtgtgatttt tggattattc
    12001 agtgtagcac caatacgcgc tcttcgcaca attgctagta tttatgtcga tatcatccga
    12061 ggaattccaa tgatggttct tgcgttcttt attttctttg ggttgcctgg tatcgttgga
    12121 tttacgattc ctgacttcct tgctggtgtt atcaccttga ctcttaacgc aagtgcttat
    12181 atcgcagaga ttgttcgagg cgggatcaac gccgttcctg tcggacaaat ggaagcttcc
    12241 agaagtttag gtctttctta taatcggaca atgcaaaaaa tcattttgcc gcaagcgata
    12301 cgcatcatga tcccatcatt cgtcaatcag ttcgttatct cattaaaaga cacaacgatc
    12361 atttccgcaa tcggtgtagt cgaattgctg caaactggta agatcattgt tgcacgtaca
    12421 acacaaagta cgtatgttta cttgatcatt gcgatcatgt acttgatttt gatcacagca
    12481 ttgacaaaat tagcaaaagt tttagaaaag aaggtgaagt aagatggcag aaaaaatctt
    12541 agttgagcat ttagtcaaaa aatacggcga taacacagta ttaaacgaca tcaatgtatc
    12601 catcaacgaa ggagatgtcg tctgtatcat tggcccttca ggttcaggaa aaagtacatt
    12661 cttgcgttgc ttgaatcgtt tagaagaacc ttctgacggt gaaattatta ttgacggcgc
    12721 ccacttgatg gataaaagca cagatatcaa cttggtgaga caacatatcg gaatggtatt
    12781 ccaacacttt aacctattcc ctcatctttc tattttggaa aacatcattt tagcaccgat
    12841 ggacctgaag aaagaatcta gagaagaagc agagaaaaaa gcaatcaaat tactgcagac
    12901 agttggtcta gaagataaaa aagatatgta tcctgaaatg ctttctggtg gacaaaaaca
    12961 gcgtgtggct attgctcgtg ctctagcgat gaaccctgac atcatgcttt ttgatgagcc
    13021 tacttctgca ctcgatcctg aaatggtcgg tgatgtcttg aacgtcatga aaaagttagc
    13081 agaacaaggt atgacgatgg ttatcgttac gcacgaaatg gggttcgcta aagaagtggc
    13141 caatcgcgtc atgttcattg acgacggaaa ctttttagaa gatggaacgc ctcaacaaat
    13201 ctttgaaaat ccgcaaaatg aacggacaaa agatttcttg aataaagtat taaatattta
    13261 aaataaatta tatatgtctt tttgactatc tttttatatc ttcttaaatg tccgcaaagc
    13321 cttattttat aggctctacg ggcattttgc ttttgtggta aacctcacat atctaggtct
    13381 atcttcttat attttcacta tcaagcgtgg ttaaaatcgt ggtaaatact tctatgcaat
    13441 tagacgctga acctctgatt ttgcggtatc tatggacgca tgagcatacc agttcattgt
    13501 gatactgata tttgaatgcc ccatgatata ctgtaaatct tttgggttca tgttcttgct
    13561 tgccagtctt gtgcagaatg tatggcgtag cgtatgcggt gtgatatgtg gcaatggatt
    13621 gtccttgtgg tgcttgttgt atttctttac catacggata aataaggcgt tataatcaat
    13681 cgcaactttg ggcttgccct tatgattgac aaataggaag ttgccccgtc cgtctatcac
    13741 aaatggttct gcctttggac gttttttcat aacctgttga aatgcctgta tcgtttcttt
    13801 gcttaatggt acttgcctta ttccgctttt tgttttaggc gtttcaatat aatagccctg
    13861 ttccttgctc tttagtaact ggtggtcgat aatcacaact tcattcttga aatcaatgtc
    13921 ggctactgtc agtccgcaca gttccgaaat acgaagtcct gtctttaaca gtatcagcac
    13981 atcatcataa tgcttgtgat acacattgtc cgtcttgatg aatgagagta aggcttgttc
    14041 ctgttcctct gtcaatgcga ctttcttttt ggtatcattt tctaggactt cactcaactt
    14101 gaaatcaaag ggatttttcc tcacataatc atcttgaatg gctatgtaga atgacgcttt
    14161 taacgagcgt ttatggttgt taatggtatt gtaggaaaag cctttgtctt tcatgcgtaa
    14221 cgcccattcc ttagcgtcag agggtttgat tgtatcaatg ctcctagcac ctaacttgtc
    14281 ctctttcaat aaccgcatga gttgttcccg ttgtttctgt gtgcttttct tcacatttgc
    14341 cctctgtgcg ttctgtttgg cataaagttg gcaaagcgtc attttcttgc cagtgctgtc
    14401 gataccgtcc tcaatatccc gtcttatctg ctgttccagt tctcgaagtg agggtttttc
    14461 ccgttttccc tttggtgtcg ggtctgtggg tgtcaatctc caagcataca catattttgt
    14521 gtttccaaat gcgtctacat atttatataa gtattttctg tctgttcgtt ggctctctcc
    14581 agtatgcagg atacgacctt tgttatcccg tcttttttct ttcatggtgt ctgctccttt
    14641 ccttgttgga aagagccttg atatgacttg tgttcatcat aacacataca aggctcattt
    14701 gcattagata gcgtccaatt tatcaatgac ctgttcaaac tgtcggcgtt taatctgtat
    14761 gcggttgcca ttcataatga gccagccagc gtccttgttt tcctctgcca atcgtcttaa
    14821 cttgttttct ccgatacgga aatactttga cgcttcttca atggtaaggg tgtatttttc
    14881 ccatacggga atatcctgtg cattactcat aaaacacccc ctcactttcg attacgtctg
    14941 ctatgtccgt acacatctgt tccacaagca tttcctgctt ttcagagagg tgggtacatt
    15001 catcaatcat tttcatgtag tttgagcctg tcagcctgtc gatttcctct atcatcacga
    15061 ttgacggcat gacctgtctt tccagccatt gcagagtgcg ttccatattg acaggtacgg
    15121 ggttcatttc aaaaggtaaa ctgtcatggg aaatgaatat ctcccacagc ggatagtcgg
    15181 gaaattgcac aaaatcagta ataaggtaaa acaccagccc atgagggtta tatgtcaata
    15241 acaattcctc aactgcctgt actgcttttg taccacgcag acgaatttca aaacgattga
    15301 taatatccgt atgtttcttt ttggttgcct gttccttttg tttttcatac aggcagaagt
    15361 attttgtgct tgcctttgaa ccgatataaa gggtgcttgc taaattacgg ttttttccgc
    15421 ttagttttcc accctgcaca ttttcataat ttttacagcg acaatccgca ccgccatttc
    15481 tgtatttttc agacaaaacg ggaatatcca acagaccaca catatcattg attgccaagt
    15541 caaaacgtcg gataacaccg tcacagtcaa gacagcggtt taaaaatgaa taccaatctc
    15601 tgttctgtgc ctgcaagaca tattccatgt tgcgtgagcc tgcccctttt aattccaaga
    15661 atacgcccat gtgttcgtca tcagaagcca tgaccttgat ttctccgtag gcgtaatgct
    15721 ctttatagcc ataataccca tagtcataat ggataaaata atctgctttc atgccaagca
    15781 cctttcggat aatttccaat gcgtcagtcg ttggataacg gacagacaga taatcaacga
    15841 gcagataaaa aggcttatcg cagatataat aattcagaca acgcaggata atattttgtg
    15901 tttccttgct ggcgttggct tttccgttct caaagcggtt gatactatat cttgatacat
    15961 gggtaagact tgcaagttct ccttgtgaaa gaccgcttgc aagacgtttg ttcttcattt
    16021 caagtgcaaa ttctaaattg ttcattgtat tcctccaatc ccacaatagc cttgaaatac
    16081 ctgtaaacct ctattcaaaa agtgtgatat gcactatctt cttaaaactt ctcattacta
    16141 ctcacaactt tgggattgta tggtttttga cttgtctttt gtgaaatagt gcccccctgt
    16201 tagataacgg gggcacagac aaggctcgca ggctcgccag ccgacaaagc atttcttccc
    16261 cttgacacaa ggtgtcatgg cggtaagcct gagaggcttc cgccaccgtg tcagaaatac
    16321 tttgtcagca agttctgttt tgccttttct aacaagttcc atttcaaccg atattcatat
    16381 ctacggattt aatcagatag gtatttcttt ttgaatgttt ttatatttca ttctattgtg
    16441 cttattcgat tacaagaaca tagtaattct tatatgctta tttgtcaata tctttttttg
    16501 cctttacaaa tcatattaag catgatagac ttataatagc gaagatataa cgagatatgg
    16561 agggttatgc aatgaaaata agccatttag gaaataatat acagaccata agaaaattta
    16621 ggggaatgaa acaacaggaa cttgcggaca aaatcggtat caatatgcag agcctttcca
    16681 agattgaaag agggttgaac tatcctgcct atgaaacgct ggaaaagata atggaagtgc
    16741 tggacgttac gcccaatgaa ttgttgtcgg gagaatggaa gtatgtcaat caatctgaaa
    16801 aggaagtctg tcagttttta agagccgaag aacgtctaaa tgcagaactg aaacatggac
    16861 actatgataa cttctttgac agcgaggaag aatggctgga atatgagtta gaaaagttac
    16921 gggaatatat tacagactat atcaacggga aaagcattga agcgtccgac ctttacccaa
    16981 tcaaagaatt tatccagcat ttgaagtttc aaaaattatt agaccgctat gatgatttat
    17041 acagtatgga tatgtttggg gaaagcatag agggacataa gtataagaca ccttatcaag
    17101 tcgtgaaaat gataaacccg aactcaaaag aagatatgga actgttacga gaagtattaa
    17161 ggaataacaa ttttgatgat gaggacgagt aacttttttc ctcttgcgag aagatacagc
    17221 aaacaaaaag cccgatcaag agtgcaaagc accaccaatg gtggcaaagc tcttgacagg
    17281 gctttttctt ttctgttgtg ggcaatcaag aggaagaaag ggataaaatc tgatttgtgg
    17341 taaatcaggc aaaggcaacg caggtatctc aatttgattt ggtacttgaa aagaacggga
    17401 gtaattggaa gattatagaa taacaaatat tggtacatta ttacagctat tttgtaatca
    17461 cgtactctct ttgataaaaa attggagatt cctttacaaa tatgctctta cgtgctatta
    17521 tttaagtatc tatttaaaag gagttaataa atatgcggca aggtattctt aaataaactg
    17581 tcaatttgat agtgggaaca aataattgga tgtccttttt taggagggct tagttttttg
    17641 tacccagttt aagaatacct ttatcatgtg attctaaagt atccggagaa tatctgtatg
    17701 ctttgtatgc ctatggttat gcataaaaat cccagtgata aaagtattta tcactgggat
    17761 ttttatgccc ttttgggttt ttgaatggag gaaaatcaca tgaaaattat taatattgga
    17821 gttttagctc atgttgatgc aggaaaaact accttaacag aaagcttatt atataacagt
    17881 ggagcgatta cagaattagg aagcgtggac aaaggtacaa cgaggacgga taatacgctt
    17941 ttagaacgtc agagaggaat tacaattcag acaggaataa cctcttttca gtgggaaaat
    18001 acgaaggtga acatcataga cacgccagga catatggatt tcttagcaga agtatatcgt
    18061 tcattatcag ttttagatgg ggcaattcta ctgatttctg caaaagatgg cgtacaagca
    18121 caaactcgta tattatttca tgcacttagg aaaatgggga ttcccacaat cttttttatc
    18181 aataagattg accaaaatgg aattgattta tcaacggttt atcaggatat taaagagaaa
    18241 ctttctgccg aaattgtaat caaacagaag gtagaactgt atcctaatat gtgtgtgacg
    18301 aactttaccg aatctgaaca atgggatacg gtaatagagg gaaacgatga ccttttagag
    18361 aaatatatgt ccggtaaatc attagaagca ttggaactcg aacaagagga aagcataaga
    18421 tttcagaatt gttctctgtt ccctctttat catggaagtg caaaaagtaa tatagggatt
    18481 gataacctta tagaagttat tactaataaa ttttattcat caacacatcg aggtccgtct
    18541 gaactttgcg gaaatgtttt caaaattgaa tatacaaaaa aaagacaacg tcttgcatat
    18601 atacgccttt atagtggagt actacattta cgagattcgg ttagagtatc agaaaaagaa
    18661 aaaataaaag ttacagaaat gtatacttca ataaatggtg aattatgtaa gattgataga
    18721 gcttattctg gagaaattgt tattttgcaa aatgagtttt tgaagttaaa tagtgttctt
    18781 ggagatacaa aactattgcc acagagaaaa aagattgaaa atccgcaccc tctactacaa
    18841 acaactgttg aaccgagtaa acctgaacag agagaaatgt tgcttgatgc ccttttggaa
    18901 atctcagata gtgatccgct tctacgatat tacgtggatt ctacgacaca tgaaattata
    18961 ctttctttct tagggaaagt acaaatggaa gtgattagtg cactgttgca agaaaagtat
    19021 catgtggaga tagaactaaa agagcctaca gtcatttata tggagagacc gttaaaaaat
    19081 gcagaatata ccattcacat cgaagtgccg ccaaatcctt tctgggcttc cattggttta
    19141 tctgtatcac cgcttccgtt gggaagtgga atgcagtatg agagctcggt ttctcttgga
    19201 tacttaaatc aatcgtttca aaatgcagtt atggagggga tacgctatgg ctgtgaacaa
    19261 ggattgtatg gttggaatgt gacggactgt aaaatctgtt ttaagtatgg cttatactat
    19321 agccctgtta gtaccccagc agattttcgg atgcttgctc ctattgtatt ggaacaagtc
    19381 ttaaaaaaag ctggaacaga attgttagag ccatatctta gttttaaaat ttatgcgcca
    19441 caggaatatc tttcacgagc atacaacgat gctcctaaat attgtgcgaa catcgtagac
    19501 actcaattga aaaataatga ggtcattctt agtggagaaa tccctgctcg gtgtattcaa
    19561 gaatatcgta gtgatttaac tttctttaca aatggacgta gtgtttgttt aacagagtta
    19621 aaagggtacc atgttactac cggtgaacct gtttgccagc cccgtcgtcc aaatagtcgg
    19681 atagataaag tacgatatat gttcaataaa ataacttagt gtattttatg ttgttatata
    19741 aatatggttt cttgttaaat aagatgaaat attttttaat aaagatttga attaaagtgt
    19801 aaaggaggag atagttatta taaactacaa gtggatattg tgtcctgtat gtggaaataa
    19861 aacacgatta aagataaggg aagatactga attaaaaaaa ttccccctct attgtccgaa
    19921 atgcagacaa gaaaatttaa ttgaaataaa gcagttcaaa gtaactgtga ttacagagcc
    19981 agacgcaaag acgcagagcc gataaaatgg ctctttgtca ataaggactg atgagttgtg
    20041 caa
//
LOCUS       DAHUVV010000277        13395 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.guided.073, whole genome shotgun sequence.
ACCESSION   DAHUVV010000277 DAHUVV010000000
VERSION     DAHUVV010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.guided.073"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(101..544)
                     /locus_tag="NK767_003021"
     CDS             complement(101..544)
                     /locus_tag="NK767_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCH6695918.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(739..2079)
                     /locus_tag="NK767_003022"
     CDS             complement(739..2079)
                     /locus_tag="NK767_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6695919.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2361..3257
                     /locus_tag="NK767_003023"
     CDS             2361..3257
                     /locus_tag="NK767_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCH6695920.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(3400..4341)
                     /locus_tag="NK767_003024"
     CDS             complement(3400..4341)
                     /locus_tag="NK767_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCH6695921.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4467..5588
                     /locus_tag="NK767_003025"
     CDS             4467..5588
                     /locus_tag="NK767_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCH6695922.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            5846..7348
                     /gene="eat(A)"
                     /locus_tag="NK767_003026"
     CDS             5846..7348
                     /gene="eat(A)"
                     /locus_tag="NK767_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCH6695923.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            7376..7942
                     /locus_tag="NK767_003027"
     CDS             7376..7942
                     /locus_tag="NK767_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCH6695924.1"
                     /translation="MVKENFVNEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQALAEKVVDLPVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELISYE"
     gene            8154..9215
                     /locus_tag="NK767_003028"
     CDS             8154..9215
                     /locus_tag="NK767_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCH6695925.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9218..10114
                     /gene="murQ"
                     /locus_tag="NK767_003029"
     CDS             9218..10114
                     /gene="murQ"
                     /locus_tag="NK767_003029"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCH6695926.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            10128..12110
                     /locus_tag="NK767_003030"
     CDS             10128..12110
                     /locus_tag="NK767_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCH6695927.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12180..12626)
                     /gene="nrdI"
                     /locus_tag="NK767_003031"
     CDS             complement(12180..12626)
                     /gene="nrdI"
                     /locus_tag="NK767_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCH6695928.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            12837..13358
                     /locus_tag="NK767_003032"
                     /pseudo
     CDS             12837..13358
                     /locus_tag="NK767_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT         4372 a         2433 c         2812 g         3778 t
ORIGIN      
        1 cacaacctct gattcacggt gttccagaag tagcttcttc ttatttcttg aagttgatca
       61 cttctggaac atcctctttt tttatttgga tggataatgg tcattccatt tctttgatca
      121 tttttttcat tttgtcgatt tcttcttctt gtgcctgaat gatttcttcc gctaattttt
      181 ttacttcagg atcttttaaa tcagctcgtt tacttgtcaa gatagcaata gagtgatgag
      241 ggatcatcgc cttcatccac gccgtatcgt tgacagttgt ttggcttcgt accataaata
      301 aggaaccggc aaaaacaaga agactagcgg ctaaaatcat agcatttatc tttttgtttt
      361 catacatttt ccacatgaac aacaacatga tgactgccat gactgcaccc atcatcaacg
      421 ccatgaatac acgtgtgcga ctgaagaaga tatgatcaag cgcataaaca ttcaaataca
      481 tcataccaaa catgactacc gtcgaaacac caatcatcaa caaaaacttc caatactttt
      541 tcatttaaaa caaactcctt tttcttcatt cctttcttct atcatgtcat gagcgtggag
      601 aacaatcaat taatctgcac agatggtttt tgaacaccgt ttattcggaa aaaagaactg
      661 caacaatttt tgtcgcagct cctttttttc aatagctcca gatattcaat ataaatagaa
      721 taaaattctc tattggtttc attcattcgt caagacgaaa gcatcattct ttttttgata
      781 actgaaataa atgaacaggt aaagcaaacc ggtcaataca gttaccccaa tggagttcgt
      841 gaataggata ccaaggaaaa tcaaccataa gactgctaat gcaaaaaagc tgattccttt
      901 gtcccataac gttcctttcc ttagtgtcag tacaaaggac gcagcactga taatgatcca
      961 gatcaataat actgtatagg aaagagaacc tgccagatag ccaaataatt tgtcaccgac
     1021 aaaataagaa agagccacac ctagataaag aacactggta caaaacagca cagcacgttg
     1081 cggtacttga tgcttgttta aaacagctag ttttctagag gaacctttcc gatctttcaa
     1141 gcggaagaac aaaattctcg aagaagcata tacacctgaa ttgatagacg agaacagagc
     1201 aagaacaatc acgaaattga cgatatctgc cgcaaatgga atattcattt tttcaaatac
     1261 catcacaaaa ggactaacgt tcgtgcctgc taaagtattc caaggaaaaa taatcaaaag
     1321 caaaaataaa ggaataatgt aaaaggaaat gattcttccc atgacgcctt tgattgcttt
     1381 aggaatcgca cgtttaggat catctgcttc gcttactgtg atagcaatca actcagaacc
     1441 gccgtaagaa tagataacta caagcaaaga actgatgatt cccttcatac cgtggggagt
     1501 aaaaccacca tgatcagtta attgttgaag ggtagggaaa acaccagtac ccaacatttg
     1561 ctgaccgaca agataagcag caaaaatgat caataggata atgacactga ttttagcaaa
     1621 ggccagccaa tattctgtct ctgcgaataa tcgtaccgaa taaagattga tgatagtagt
     1681 taaaacagcc aacataaaaa caaataccca agctgggatg tgggggaacc acaactgtaa
     1741 aaaggaagca gctgcaacag cttcagcaat gatattgata atccacatgg accagtagac
     1801 ccaatcggta aaatcggcag catgagcacc tagatatgga cgaatcaatc cagataatcc
     1861 gtggtgcgta tcaccaccac ttaaaacaat ttttcccaga cctttcatca cgatatataa
     1921 aattatgccg ccaatcaaaa aagcgatcaa tacagaaggg ccggcgattc caatcgcttc
     1981 gccacttcct ttaaatagac ctgcaccaat cgctccacct aaagctagca tggtgatatg
     2041 tcgggagcta agttgttttt tcaatttttt atcttccata aataacctct ttcttttaaa
     2101 ttcacaatca aaatgcatag taagaataaa tagtaagaat aaatagtaat aataataatg
     2161 actaatgata acaaattttc agataattac aactattcag aaaacttttg ggcatatttt
     2221 aatggaagaa tgatttattt catcgtattt tcatagaaat gttaaatttt tataaaaata
     2281 ataggaagcg ttttactggc tttttactat ttaaacaggc tatcatagga aaaagtgaca
     2341 gagaaagtga ggcggaacag atgaacaaaa acgacgtatt gctaattgcg aaaaataaat
     2401 cactttattt ttttccttcc atccgctctt tagatgtttg gtccggaaaa gatttactac
     2461 agtcggaatt cattaaatca gctcgacaac agttggcaaa ttatcagctg atcgtttttt
     2521 tagattatgg atttgatatg gagatggctc gacgcgtgcg tccatttacc aatgcaaaaa
     2581 tcgtgttatt tttttggaac cactttaaag cagaacacga ggcgatgctg aagcaggcac
     2641 aagatgaacc ggcaatcgat gagatttatc attttgattt tttagaagcg aaagaacttg
     2701 gactaaaaca taatagttcc ttttattcga aacatatgga tttttcttct agcgaaccaa
     2761 agacggatct attttttggt gcaacaaata acggcagaaa agaacgggca gaaaaatata
     2821 agcaggaatt tacaaaaaga ggtgttacga ccaattactt tattcttccg cttcgaggaa
     2881 atgaacaacc tggatacctt acttatgaag aatatctgaa aaaaaccggg gaatcgaggg
     2941 ggattctaga actgttgcgg gaagggcagc aaggagtgac tttgcgaacg tttgaatcta
     3001 tgtattttca aaagaaacta gtgacagata atcaagccat agctcattat cagttttatc
     3061 attctgaaaa tatttttttg cttcaagaac gtgatttaga tgaattaccg gaatttttgc
     3121 atacgccatt tcaaccaatc ccatcagaac tgttggaatt ttttgatgca gaaaattggg
     3181 caaaacgttt tttcactact gactccgcta tttatgaaca atatgaatac gatctttcct
     3241 ctgttaaaaa taaataaaag tgccaaccaa tcatagatga aaataaacaa gcgcaattct
     3301 tatggcaaca tgagaattgt gtttttcttt ttgaactttc atgggggtaa aaaaatgaga
     3361 accagctgct gatccagctg attctcatca gagagccttt tattctgcgc caaggattcc
     3421 ttcttttgca tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt
     3481 ttgtcctttt cgccaaacgg caccattgat gtagtcgatt tcagtcaaac gatggttttt
     3541 gatcaggtct tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga
     3601 agcaatatgt tggtacactt cttcttgatc taatgtgatt ccttcttttg ctgcgataga
     3661 agcaaactca gatacaatcg ttcgaaccat gccctctgcc gctttttgcg cacctaattc
     3721 agcaatatta caatccaata tcgtgcataa tccatttaat gttccgttta cacaagcttt
     3781 tcgccaaatg gaataacgaa cattatgact atagattgga ttcaattttg cttcttgaaa
     3841 gacttctaca actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat
     3901 ttctccatca cctaataatg tgacttttcc gggaccagct agaccagctg tccacatcgt
     3961 gatcccaaat aagatatttt tctttggtac atatttttcc agtacatctt catgacctaa
     4021 tccattcagt aaacacaaaa cataagtatt ctcagtaata atggattgga tcgaacgaaa
     4081 catatcatct aattgattcg cttttgtcaa ggctacgatc aaatccacgt gtttgttgct
     4141 ttcgatgatt tcttctggtg aatagattgg cagttttgca actacttctt tgccattaaa
     4201 gtcagcaatc aatccatttg tacgaatggc ttcaatgtga gcaggccagc gatcgatcaa
     4261 gacgacctca tttccgcttt gatgcaacat taatccgatt cggctgccca ttgctccggc
     4321 tccagctatt gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta
     4381 tatagaaaaa cagaagaaaa tagcattgtt ttgtttgaaa aaagtgaaaa aaagtgtctt
     4441 acttaagaag aaagtgaggg atatagatgg atattttggc ggcaatcgat tcatttaaag
     4501 gaagtgcttc ttcatttgaa ttaaatcagg cagttctaaa tggttgggaa gaccgtaaag
     4561 gaagaaaaat caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt
     4621 acgcgctcgg gggaacctgg cgtacgattg atgggatgga tctcttattt cgtcagcgaa
     4681 aaatcagtta tttattgacg aactggcaag gaaaaaatac cgcagtcatt gaatctgcac
     4741 aagtattagg attagattta cttgataaac caacggatca aaccatccga cttgcttcta
     4801 gttatgcctt gggagagctt atcaaagata cactgcttca agaagtggaa caaatcattg
     4861 tcactttagg aggaagcgga tgtagcgatg gtggtctagg attactccaa tctttgggtg
     4921 cttctttaga agggtacaca gaaggaaatc ctttgttatc tactagaaaa cttcatttgg
     4981 atcgaaggaa cgttcttgtt tctaaagtaa aagtgatcat cgctgcagac gtgaccaacc
     5041 catatacagg tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaacggata
     5101 cgacgttagc tttcttggat aaacaagctg aaaagattgc tcagcagata aaagatgttt
     5161 gggaggtgga tttagatgac atcccaggaa gcggagcagc aggcggtatt ggcggagccc
     5221 tttttttgtt aggagcgaag atggtctctg gttttgagtt ggtcagccaa atcactggat
     5281 tagaaaaaca gatcgaacaa gcggatgtca tcattacagg agaagggcgg attgatcagc
     5341 aaacaatcca cggaaaagtt ccatatggcg tagcagtgct tgcaaaaaaa tatcgaaaac
     5401 caataatcgc tatttgcgga agtagagacc gagagctgga aggactcttt gattttctcc
     5461 ccattgtctt aagcattcaa ttaggaccaa tcgatttaga ggaagcaatg gaacacaggc
     5521 aaacattaga aaaaacagaa atcttaggac agacactcag cagatttttc aacattctcc
     5581 cgaagtagtt gacaaaaaca atttctttat gctatcgtaa tttcaaatat aggacaaagt
     5641 gagggatccg tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct
     5701 tgtggagaca agggagcagt gtgcagattt tcgggattgg cttggttctt tcgatgtatg
     5761 ggtctttttg tgtgccccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg
     5821 tcttttttta cgaggtgatg gaataatgtc taaaatcgaa ataaaaaatc tgacattcgg
     5881 ctatgacagc caaggcacat tattatttga acaagccaat ctaaactttg acacacaatg
     5941 gaaactagga cttatcggac gaaacggtcg aggaaagaca actttactga atatcctgca
     6001 aaacaaactc ccttatcaag ggcaagtgat ccatcagcaa gcgtttgtct atttcccgca
     6061 gcaaacaaaa gataaagagc gtttgaccta ttatgtgtta aatgagatta tggattttga
     6121 gatatgggaa attgaaagag agctccaact gatgcaaaca gatcctgaaa tcttatggag
     6181 agaattcagc acactatcag gaggagagaa gacaaaagtc ctactggcac tcttatttgt
     6241 ggatgacact catttcccgt taatcgatga accaacgaat catttggata tctctggcag
     6301 aaaacaagta gcggcttact tgaaaaagaa aaaacaagga ttcatcgtgg tgagtcatga
     6361 tcggggattt atcgatgaag tagtggacca tgttttagca atcgaaaaga gccaactgga
     6421 actttatcaa gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga
     6481 aatagctaaa aatgaaaaat tgaaaaaaga agtcagcagg ctaaagaaaa cagcagctga
     6541 aaaagcagaa tggtctcgtt cccgagaagg ggacaaaaca aagaaaaaag tcggattcat
     6601 cgatacggaa tctaggcgag tgaataaagg agcagtaggc gctgatgctg cacggacgat
     6661 gaaacgatct aaagcaatcg tgaatcggat ggaaacccag atcagtgaga aagaaaaatt
     6721 attaaaagat atcgagtata tcgatgcttt gacgatgaat agccaagtgt ctcatcataa
     6781 acggctgtta agcgtagaaa atcttcaatt agggtatgaa aatctgttat tcgagccagt
     6841 tcattttacg atcgaacctc atcagcggat ggcaatttca ggtcctaacg gcgcgggaaa
     6901 gtcatccatt atccattatc ttctggggac attcaacggc aaggttgtag gagaaaaatc
     6961 ccagcccaaa catttgacca ttagttacgt acgccaaaat tatgaagaca atcgagggac
     7021 attggcggaa ttcgcgaaga aaaaccgagt agactatcaa gcatttttga ataatctccg
     7081 aaagcttggg atggaaagag atgtttttca taataaaatc gaacagatga gtatgggaca
     7141 acggaaaaaa gtggaattgg ctaaatcttt atcccaacca gctgagctct atatatggga
     7201 tgaaccattg aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgattttgaa
     7261 cgtgaaacct gctatgttac tagtggaaca tgatcaaacc tttctagata aagtatctac
     7321 tgagattatt tctcttgaga aaatctaaac cttgtaatct caaagacaac aaggtatggt
     7381 taaggagaac tttgtcaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga
     7441 aacaacttgg aacgcacaag gattagtttg cgggcgagca gatgtgccgc tgactgaaaa
     7501 aggacagatg caggcacagg cactagcaga aaaggtagta gatttgcctg ttccaatcac
     7561 gaaaatcatc cattcccctc ttcaacgagc aagagataca gcacaagcag tagttgatcg
     7621 tctcaatttg ccgatgaccg ctgatgagcg attggtagaa atggattttg gggattatga
     7681 cggactgcca agcaaagatg aaaactttca aaaagcgcgt ttagcgtttg ctgtacgttt
     7741 cccaaatgga gagtcggtct tagatgtgta cgcacggatc gttcctttat tgaaagaatg
     7801 catggaagac gaagaaaatg tctacttgtt agtctgtcat aatgccttga taagagtgat
     7861 caatgcttat tttcatccaa tgcctaatga agggttcttt acattcatgg tagacaatac
     7921 agaattaatt tcttatgagt aattagactt ttgtgttatt taaataaaat gtttcaaggc
     7981 actgcttttg cttagcagtg ccttttttgt ggctgtgtca attcttttgg cattataaat
     8041 ccaattttta gaaataaaat ttaattattt taacaaaagt attgaaattt tgtttcttta
     8101 gatatatact ctagtcatag aaatgaagac ggatacagaa aaagagggat aatatgtttg
     8161 gcttttcaat atttatgaat gattctttgt cagaagagaa gaaacagtat atccgccaaa
     8221 tggcatccaa tggctttgtc ggtattttca cttccatgca tataccggaa gatgatgttt
     8281 cggcttatca aaatcgattg atcgaactag gaagcgaagc gaaaaagaat aaactagagt
     8341 tgatggtcga tatttcagga gaggcattga aacgagctgg cttttcatta gaagatctaa
     8401 cacccttgaa agcaattggt gtaacagggt taagaatgga ttatcatatt tctaatcagc
     8461 agatcgccga ctggtcacat caattgaaaa tcagtttgaa tgccagcacg atcacgccta
     8521 atgatatcga tgagttgaaa gaagcagcgg ctgatttctc acagattgaa gcttggcata
     8581 attattatcc acgtcttgaa acagggttgg acaaagaatg gtatcaggaa aaaaatcaat
     8641 ggctcaaaac tcaaggattc cttgtacaag gctttgtacc aggagataca gagttgagag
     8701 gtccactgta tcaaggatta ccaactttgg aagaacatcg agggatccat cctctggcag
     8761 gtgctcttga tctattagca agtaacacgg atattgttta tatcggagat gcaggtctgt
     8821 cagaacacgt tcaggagcaa tttgcttctt tccaaaaaga acagacagtc ttgctccata
     8881 cggaacctgt agatgaagtt ttttacgaat atatattagg aaaacatacg aatcggcaag
     8941 atgatgcaag agatgtcata cgaagcgcag atgcgcgttt tcgagagatt ccgccaatcc
     9001 cagtaagaaa tacagctaag cgtatgaaag gatctattac tttagataac gaaaagtatt
     9061 tgcgctatat gggtgagatc caattaacta aatatgattt gccagcggat gaaaaagtga
     9121 atgtagtagc caaagtcatc aaagaagaac taccgctgat caaccaaatc aaagctggta
     9181 tgaactatca atttataaga aaagaaggaa gataaaaatg agcaaaatca atttagagaa
     9241 cctaacaacg gaacgcagaa accaagaaac ttttggatta gatgaaatga gtatttctca
     9301 agcagtccaa ctcatgaaca aagaagatca gaaagtggcc gctgctgttg aaaaagaact
     9361 gccaagtatc gagccagtga tcgccaacac gattgaatcc tttaaaaaag gtggtcgctt
     9421 gatttatatg ggagctggaa cgagtggtag acttggtgta ttagatgctg ctgaatgtgt
     9481 accgacattc ggtgtagaac cggaaatggt cataggccta attgctggtg gagaagaagc
     9541 aatgactgta gccgtcgaag gagccgaaga ttcttatgaa ttagggcaag aagatctaat
     9601 caagctgaat ttaacaaaaa atgatatagt tattggtatc gcagctagcg gtcgtacacc
     9661 atatgtgatt ggtggattag attacgcaaa atcgattggt gctgttacag gaacgatttc
     9721 ttgcaataaa caagcagaaa tcagcgagca tgcggatttc ccaatcgaag tcgatgcagg
     9781 accagaattt ttgacaggct ctactcgatt gaagtcagga acagcacaaa aactgatttt
     9841 gaatatgatt tctactatca gtatgatcgg tatcggtaaa gtattcaata acttgatggt
     9901 cgacgtcaaa ccaacaaatg aaaaactaat tgaacgctca aaacgaatca tcatgcaagc
     9961 gacagaagct gactacgaaa cagctgaaaa gtactttgag gaagcagatc aaaatgtcaa
    10021 actggctatc gtcatgattt tgacaaatag tgataaagag gcagctcaag caaaattagc
    10081 tgaagcaaac ggttttgtaa aaggaacctt atgaggggga aaagaccatg gcaaaaaacg
    10141 aattgacttt ggaagaactg gcaaaacaaa tttatcaagg agcaggcggt atgggcaatg
    10201 ttgaaagcat tgtccactgc atgactcgtg tacgtatgaa ggtcatcaat gatgatcttg
    10261 taaatgtctc ggaattaaaa gcgattccag gagtacttgg cgtagtagag gatgaacagc
    10321 tccaagtcat catcggacct gggaaagtga acaaagtagc gcaggaaatg gttgacttag
    10381 caggcgtgaa attaggagaa aaacttcctt ctgctagtgc aactacagca agttcttctg
    10441 catctggaaa agaccgtgta aacgaacggg cacaagaaat gaaagcggca caaaaatcga
    10501 aacaaaaacc ctctaaaatc aaatcggtat taaaagacat ctctaatatc tttgtaccaa
    10561 tgatccctgc atttgtcggc actgggatcg tggcagggat tgccgcagtt ttagcaaact
    10621 tagtcacagc tggaacacta gacgcaggaa catggcaaca atatatcgat gtgatgaaca
    10681 tcttgaaaaa cgggatgttt agctatctag ttatctatac aggtatcaat gctgcacaag
    10741 tttttggtgc aacgccaacg cttggtggag ttatcggggc tgttgtgatg ctgacaggga
    10801 tgaatccaga agcaccactg aaaaacttgt ttaccggtga agctcttgca actggacaag
    10861 gcgggatctt aggtgttatc ttcgcagttt ggttattgtc aatcgttgag aaaaaactgc
    10921 ataaagtagt acctgatgcg gtagatatca tcgttacgcc aactctttcc ctgattgcca
    10981 tcggtctgat cgaaattttc ttcatcatgc ctttagcagg gttcatttct gacggcatgg
    11041 tcggtgggat caactgggta cttggtgttg gaggagcatt tgccggcttc gttctaggta
    11101 cattattctt gccaatggtc atgttcggac tacatcagat cttgacgcca atccatgtac
    11161 aaatgattga tgaaactggt cgtaccttat tgcttccaat ccttgcgatg gccggagcag
    11221 gacaagtggg tgcagcattg gcattatgga tccgctgcaa aaaagacaaa gaattaacag
    11281 aaatgatcaa aggggcttta ccagtcggaa tcttaggaat cggcgaacca ttgatttacg
    11341 gagtaacatt acctctagga cgtccattta ttactgcttg tatcggtgga ggtatcggtg
    11401 gggcagtgat cggaatgatc ggaaatgtcg gagcaatcgc tattggacct tcaggagccg
    11461 ctttgattcc attgatctct gatggtaaat ggttaggcta tatccttggt ttattggctg
    11521 cttacgctgg tggcttcgtt gctacattct tcttcggtat tcctaaagac cgtttagaaa
    11581 aagaagaact agcgaccgaa acagccacag taaatgaagt acctgaaaaa gcagtacctc
    11641 aagcttcagc tgaaagtgtg actatcttat catcagttgc tgacggaaca gttgtttctt
    11701 tagataaagc aagtgaccca gtttttgcac aaaaaatgat gggtgaaggt tactttgtag
    11761 aaccagaaaa cggacatatc tattccccgg ttactggagt aataagttct gttttcccaa
    11821 caaaacatgc tatcggcatt acgacaccaa atggcttaga aatcttgctt cacatgggca
    11881 tcaacacagt tgacttaggc ggtacgccat tcgatgtgaa agtaacagaa ggacagcaag
    11941 tgacacctga tacgcttgta gcagaagctg acttagaagc aatcaaagca gcagacaaag
    12001 aaacctccat gatcgtcttg gtaaccaata tggatcgtgt aaaaaacttt gtcttggaaa
    12061 aaacaggtaa agcgaaagca aaagcacctg tgatggatgt caaagcttaa taagaataga
    12121 aacaatagag aacataaaaa gaagaagccg aaatttattt ttcagcttct tcttttgtgt
    12181 tatttatttt tttctgccaa aatttgataa acgcgtgtca catcagatgg tgttccccgt
    12241 aattcataat ctgccaagaa aggaaagccg aatctttgtg cgtattgttt ggctgtcaga
    12301 caatattgat tgttgaagtt tttattaccg ctaccgacta caccaaggca aagtttatga
    12361 ttgtcttcgt attccagata ttcgcgcatc gtttcggtca agatttctgt gtcaccgttg
    12421 tctaacccat tcccgccgtc taaataagta ggaacgaaag taaaaaatgg ttctgtttct
    12481 ttcgtaaaat cactattttc atggatttct tttaaagtaa cttcaggatt gacaggatct
    12541 tctgcatgca ttttttcagc atattctgct agatgtttgg caaaagcacg cgtatttcct
    12601 gaaatcgata tatataaaat gttcatggat catcccccaa ttctttccct ttagtatagc
    12661 atctttttaa gtttttttgg tggtgtaatt tggaaaggga ctaggcgctt tgcttgtata
    12721 atgagaaaaa agtcggtaga attatatttc tattaaagtt tttattaatt aagtaaaaat
    12781 aaaaaaggtt tgttatactt tttgataaaa gcggttttac tttggaggat gcttacatgg
    12841 atacagagca acagaaaaaa ggaattggtt tgattccact tgctgcctta gtgattggtt
    12901 cagcgattgg aggtggcgta tttggtatta tgacggacat ctccggatcg gctggtggcc
    12961 cagctatctt cagctgggta ctcgtaggaa cagcaatgtt gatgctctcc ttatcgatca
    13021 ataatatcaa taaaaaacgt ccagatttaa aaggcgggat ctttagttac gcagaagaag
    13081 gttttggccg tttcgctggt tttatcagcg gttggggtta ctggctcaca tgctggttag
    13141 gaaacgtggc gttcgcaaca ttgttgatga gtgcattagg ttatttcttc cccgtattcg
    13201 aaggtggtca aaatgtggca tcgatcgtga tgagtacgat cttcttatgg ggatatgcat
    13261 ggctagtgaa tcgtggagtt gaaaatgcta gctttgtcaa tgctatcgtc acattttgta
    13321 aattagtgcc attattttta tttattgtag tagctatgta agcgccccat agaacagtac
    13381 ctgtacgtca aataa
//
LOCUS       DAHUVV010000278        13212 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.guided.076, whole genome shotgun sequence.
ACCESSION   DAHUVV010000278 DAHUVV010000000
VERSION     DAHUVV010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.guided.076"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            complement(204..2162)
                     /locus_tag="NK767_003033"
     CDS             complement(204..2162)
                     /locus_tag="NK767_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCH6695929.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="NK767_003034"
     CDS             complement(2159..3031)
                     /locus_tag="NK767_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCH6695930.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="NK767_003035"
     CDS             complement(3373..4245)
                     /locus_tag="NK767_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCH6695931.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4941
                     /locus_tag="NK767_003036"
                     /pseudo
     CDS             4373..4941
                     /locus_tag="NK767_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            5008..5745
                     /locus_tag="NK767_003037"
     CDS             5008..5745
                     /locus_tag="NK767_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCH6695932.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5976..7244
                     /locus_tag="NK767_003038"
     CDS             5976..7244
                     /locus_tag="NK767_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCH6695933.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7731..8195
                     /locus_tag="NK767_003039"
     CDS             7731..8195
                     /locus_tag="NK767_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCH6695934.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8179..8808
                     /locus_tag="NK767_003040"
     CDS             8179..8808
                     /locus_tag="NK767_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCH6695935.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9162..10640
                     /gene="msr(C)"
                     /locus_tag="NK767_003041"
     CDS             9162..10640
                     /gene="msr(C)"
                     /locus_tag="NK767_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCH6695936.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10687..11133
                     /locus_tag="NK767_003042"
     CDS             10687..11133
                     /locus_tag="NK767_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCH6695937.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11332..11847)
                     /locus_tag="NK767_003043"
     CDS             complement(11332..11847)
                     /locus_tag="NK767_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCH6695938.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11964..13166
                     /locus_tag="NK767_003044"
     CDS             11964..13166
                     /locus_tag="NK767_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6695939.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4115 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcact
     4861 gttacgaaag atgtcccaga caataccatc gttgcaggaa tccctgccaa aatcgtcaaa
     4921 cctgtcgaac ggattatttg aacagtaaga tgaaataaga gatagtgagt ataagcaaga
     4981 gatttttcaa aacaggagga gaatagaatg aatgatttat cagaaaaagt gattgtcatc
     5041 atgggcgctt caagcggaat cggtgaagca acaacgaagc tattagcaga aaaaggggct
     5101 aaactagtta tagctgcacg ccgagaagat cgcctaaaag caataaaaga atctttacca
     5161 gaagctgagc tatatattca aacagcagat gtaagagatt ttgcacaagt ccaagcagtt
     5221 atcgatttag cgatggaaaa gttcggtcga atcgatgtat tgtacaataa tgcaggaatc
     5281 atgccaaccg ctccattagt tgaaggtcac cgtgatgaat ggcaaaatat gcttgatatc
     5341 aacatcatgg gtgtgttaaa tggaatttcc gccgtacttc caatcatgga aaaacaaaaa
     5401 tctgggcata tcatttcaac tgattctgtg gcgggacatg ttgtttatcc tgattccgcc
     5461 gtttattgcg gaacaaaatt tgctgtccgt gcaatcatgg aaggattacg tcaagagcaa
     5521 cggcaaaata acatcaaatc tacgatcatc tctccaggag ctgttcaaac tgaattatat
     5581 caaaccatca gcgacaaaaa ggctgctctt gaacttcatg aagcgcaaaa agaatgggga
     5641 ttaacatctg aagatatagc ttctgctgta gcatttgcga tagaaacgcc agaccggatg
     5701 tctgtcagca acatgattat ccgtccgacg acacaggaag tataatccat aacggataat
     5761 atccagagag aattacttgt taaatagtta ttcaaacgac catcaattta aggcgtgaat
     5821 ggagaagaat attcttcatt tacgtctttt tatgcagaaa aagcaaattt attaaaaaga
     5881 aacaaatgtt atttaaatat ggacattgac ttgcttttac ttttttagtt acactgttat
     5941 ttaggaaaag tttcataatt caaaggagaa aaaagatgaa agcaaagaat ttattattta
     6001 gcgttattct tttaggaaat ctagcagttg ccaacacaac agtgctagct gaagaatcga
     6061 cagaaactgt cgcaactcag acatcgagtt cttcagaaac aggaattgaa aacgatacag
     6121 aaagtacttt gcagtcagct actgtctcag aaaatacaat ttcaaccgaa gaaccaacaa
     6181 ctgaaacaac agaacaaaca caaaccgaag acagtacatc agcagaagct cagaacaatc
     6241 cagagaatgc agaagaaatc acgaaccaat tgacagatgg gcaaacattg acacataccc
     6301 atcaagcaga tttgtctgaa ttcgaaaact atactggcta tgctttttat gctcgctcat
     6361 ctgctaattc tcaacaagcg ttcattgata gtattgcctc aactgcacaa agtttggcaa
     6421 gtgccaatga cttatatgca tcagtcatga ttgctcaagc catcgttgaa agtggctggg
     6481 ggaacagtac tttggcttct gcccctaatt acaatctatt tgggattaaa ggaagctata
     6541 atggccaatc tgttacgatg cctacttcgg aatatgtgaa tgggcaatgg attacggtga
     6601 atgccgcttt tagaaaatat ccttcgtaca aagaatcatt gcaagataac gtaacggtat
     6661 tgaagacgac ttcttttcag ccaggcgttt actactattc cggtgcatgg aaaagcaata
     6721 cgaactctta caaagatgct acggcttggc taacaggtcg ttatgccact gcgcctaatt
     6781 atggttctac attgaacaat gtaatcgaaa cctataatct gacacagtat gatacagcac
     6841 caacttcaac ttctccgatg tatcgtctgt ataaccgtca tacaggagaa catttatata
     6901 cattgaatgc cggagaaaaa aattatctgc caactgtagg ctgggaatat gaagggattg
     6961 cttggcaagc accaaacagc ggacaacctg tctaccgttt atacaatcct tacagtggag
     7021 accatcatta cacaatggca caatctgaga tcaactcttt aacaaaaatc ggttggcgtt
     7081 atgaaggtct aagtttttat tctggtggtt ccaagccaat ttatcgacta tttaatccaa
     7141 atgaaaaaac tggtacccat cactatacct tgagtgctaa ggaaagagat tggctaacac
     7201 cgatgggctg gagatatgaa ggaataggat tttatggtta ttaaaaagag gtagtgaaaa
     7261 agctgccctg catcaagtag taagaacagc caaacaaaaa cagattttca tgttttttgt
     7321 ttggttgaac ttattaccgc agatgcaagt ttttgaacac cgtttactca aaaaaggttg
     7381 tgacaacatc ttgtcataac ttttttatta ctgtttaagc atactgatcc aatttctaat
     7441 attcgacggg attcttgcaa aaggcgattt cggaaatttt tggcggtgta aaattatcta
     7501 ctgcagaaga gctatcttct atagtttgat aagaatcaat aatcttggtt ttagcgttgt
     7561 gctttcttta tcattttttc cgttcattct acaaaaaatt tcttttctct atacttttct
     7621 attgagagcg ttttttttat tacacagctg attctttgac ttttttcttt ttttattcta
     7681 ttattgacaa gtcaatcgtt gacgagtcaa ttattagaaa ggaggaaatt atgtctttag
     7741 aagagattag tcacctttta tatcaaatca aattagccga tcaaaaaatc gtccagatct
     7801 ttgaagaaaa tatcggtata agtcttacta gatacgagat catgatgaga ctgaaagaag
     7861 aaaatccatt gctacaaagc agtttgcaag aaaagttgca gattgatcaa ggagcagtta
     7921 ctcgtcatct gaaggtattg gaagcaaatg gctatgtttc gcgtcagaga aatcctcaga
     7981 acaaccggga agtgtatgta gcgctaacag aaaaagccca gaagaaaatc aagcattgtg
     8041 aaatggacca taaagataca ggttccgtgc tatccccggc tttcagcgat gtagaaatga
     8101 aacaatttct aacactttta gataaattga atacccaact ttgcaatata acaacagaaa
     8161 cgaaagaggt taataataat ggaacaaaaa aatgattttt caacaattat gactggtaga
     8221 agatccgttc gcgtatatga tgaaacatac aaaatcccac acgaagaaat gctggaaatg
     8281 atcaaagaag cgacgactgc accttcttct gtcaatatga aaccatggcg ctttgtggtc
     8341 gctgaaagcg aggaagcaaa agaaatcttg cgtcctttga tccgtttcaa tacgagacaa
     8401 aatgatactt cttctgcaat ggtcatgatt tttggcgata tgcaatgtta cgaatatggc
     8461 gaagaaatct acaaccaagc ttacgaatca gggaagatgc caaaagaagt cagagatcaa
     8521 caattagctg cgatcattcc ttattacaag agtttttcaa gagaagaaat gaacgatgta
     8581 gtcaaagtcg attctagctt agcggctatg caattcatgt tagttgcgcg tgatcatggc
     8641 tatgaaacaa atccaattgg tggattcgaa gcagatcagt tggcagaggc atttggtttg
     8701 gataaagacc gttatgttcc agttattatt ttatcaatag gaaaagcggt agaagaaggt
     8761 tacgagtctg ttcgtttagc accagaaaag atcactatgt tcgaataaaa ggttttcagt
     8821 tactattcag tagctattgc tttttttgaa tcgtgtgtgg taacctaata aaaaaggagg
     8881 tcggaaaaat gactgcatcg atgaaattac gtttcgaact tttgaataac aactaaataa
     8941 attcaaaaga catgtataaa caacaatttc atggatggag tatgtccatt ttttcgaacg
     9001 acagtagaat aggtgaaagc tatgacagat ttggtgattt agtcatagtt atttcgttca
     9061 gagtaggaaa agatgtgatg cagttttttt gcaaacagat ttttctgctc ttttttagta
     9121 tcgataattg ttgttttaca tgaaatagga gtgtaaaaca tatggaaaat ttagcagtaa
     9181 atataacaaa tctacaagtc agctttggca accagctaga actatctatt gattctcttc
     9241 gtgtctatca gcaagatcgt atagggatca taggagaaaa tggtgtcggt aaatcgactt
     9301 tgctcaaact aatagccggt gaactttttc ccgatcatgg gaaaatccaa acagagatca
     9361 ccttcaacta cctgcctcaa ttaacctatc tcgctgaagc aaaggaccta aatttggaat
     9421 tagccagtca tttccagtta agactggaag aaacttcaga gcggaaatgg agcggagggg
     9481 aagaacgaaa gatcgagttg atacgtcttc tttcttctta tgaacaaggg atgcttctag
     9541 acgagccaac aacccatcta gatagaaaaa gtattgatcg actgattgaa gagcttcgtt
     9601 attattatgg tacgctggtt tttgttagtc atgatcgcta ttttctagat gagctagcat
     9661 cgaaaatctg ggaagtaaaa gacggagaaa tccgagagtt ttcggggaat tatagtgcct
     9721 atctcactca aaaggaattg gagaaaaaga ctcagctacg agaagcagag tcgatcatga
     9781 aagagaaaaa acgattggaa aaatcgatcc aagaaaagaa aaaacaagcg gaaaagttgg
     9841 aaaaagtgtc cagtaaaaag aaaaagcaac aaatcagact ggatcggttg tcttcctcta
     9901 aacaaaaaga cagtgtacaa aaagccatcc aaaagaatgc gaaaacatta gagagaagac
     9961 tccaaaaaat aggagaaaca accaagccgc agcagatgaa acaaatccgt tttccagtac
    10021 caaaatctct tgaactccac agccgctatc ccatcatggg acaaaatgtc caattggaaa
    10081 gaagtgggag aacattactg gtaaatggtg attttcagtt ttctttaggt aaaaaaatcg
    10141 cgattgtcgg tgaaaatggt tcaggtaaga caaccttatt agaacatatc cgcaaacaag
    10201 gagaaggaat ccttctctct ccgaaagtaa gctttcaagt atatcagcaa aagggttatc
    10261 aaatgacatc tgaagaatcc atcattcgtt ttgtcatgag acaaacagag ttttcagaat
    10321 cgcttgtccg tagtttgctg aatcacttag ggtttgctca ggaaactttg acgaaaccgt
    10381 tatgtacatt aagtggggga gaagcgaccc gtctgacgat tgctttgctt tttactaagc
    10441 caagtaatgt gttgctgtta gatgaaccga ctaattttat tgatatggca acgatcgaag
    10501 ctttagagaa gctaatgcaa atatatccgg gaacgatttt gtttacttct catgattctt
    10561 actttgtcga gcgtacggct gatgaagttt atgaaataaa agggcagaaa ataaaaaaag
    10621 tacttacgag aaatttttaa atgtgctacg atgattttcg tgacaaattt aaaggagtgg
    10681 tacagattgc ctacaaataa aaaagaagga ataattttta caacaatgat gtgttttttg
    10741 atggtcttgg ggatgagcgc gtataattta tggctgcatc aagatcttca tttatcaagt
    10801 ttgttgattg gtctagtgcc tggattcgtg gtggctttca ttttggatgt cttcatcatc
    10861 ggtgtgctgg cgaaaaaaat tgcctttaaa ctaccagtaa atccagaaag caaggtccaa
    10921 atgatcttaa ctatttcatg cttgatggtt ttaggaatgg tgacatgtat gtctttattc
    10981 ggtgtgatca tggaacaagg aatacctgat caattattct ctgcttatat gaatgcttgg
    11041 aaaatgaatg tgattgccgc tttaccgttg cagttgatca tcgttggccc gctttcaaga
    11101 agtgtgctaa aagcaattca aagtaatgaa tgatggaaca ctgttatttg aaaaaaggcc
    11161 ttggcatgct ttttgtcata gtctcttgaa tgcagttcta gaaaaatagt gatccgaacg
    11221 ataggttttg gcctgtcgtt cggatcgctt tttcttttcc aactgtattg ctcttttatt
    11281 gaagataatt tgctcttcaa tagatatttc tagaaaggtt ctgtttatcc cttactcaaa
    11341 aaactgctgc atttgttctc ttacttgctg acaccattgg agttcctgct gacaaagcat
    11401 aattctgcgc tggatcacaa cataacgtcc aaattcttgt ttcttgtcta gcgagaggtc
    11461 attcaatttt ttttgatttt tttgcatgat tttgttcagc tgttgttctc tagtgaatag
    11521 cagtccagta gctgttgttt tatcgagcgt cgaaaaagca tagatttttg ctagaaattc
    11581 atctttttgc gtaggagcgg gagtttcctc tttgatccat tcctctacaa gagcacgtcc
    11641 ttcatcagtc aagtggtaga tctttttttg agagtgctcg tctttgtcca aaacggtcac
    11701 ataaccctgc tggtctaact tggcaagcgt tgtatagatc tgactgtgat gggcttccca
    11761 aaaaattgtg atcagctgct tcaattcata cccagaacgt ggtttggata aaagcacgca
    11821 taataatata taggctaaag tattcataaa aatcctacct ctcgtcttat tttctttatt
    11881 atatcacagt aaagtttcca cgttttacat gtgcatattg acatgtaaaa taaatcatgt
    11941 atgatgctaa tagagagaaa gggatggaaa tattggagaa tcaaatagat atacaagaac
    12001 aaacaaatga agaaacaaag gggaaaagtt cagcgctagt tgtagcagct acctgtatga
    12061 tcgcttttat gggagtgggt ctagtagacc caatcttaaa aacgattgct gcacagttaa
    12121 acgctacgcc agctgaaacg accctgttat ttactagtta catgttagtc actggtgtgg
    12181 tgatgctatt tacaggtttc ttatctagtc ggatcggttt gaaaaaaaca ttgatgacag
    12241 gattgtttat catcgttgta ttcgctggtc ttggcggatt atctgggaat atcggaatgc
    12301 tgattgggtt acgtgccggc tggggtgtcg ggaatgcttt atttgtctct actgctttag
    12361 caacgatcgt cagtatttta gttggtaaca cagaaaaagc aatcatgatg tatgaagcag
    12421 cacttggctg cggaatggct gttggccctc tagtcggtgg ggtcttgggc agtggttcat
    12481 ggcgttatcc attttttggg gtagcatttt tgatgttttg cggttttata gccctagcgg
    12541 tacttttacc tgaaactgag aaaccaaaga gaaaagtagg cctttgggaa ggtgtcaaag
    12601 cgttaagcaa tcataaactt cgttcagttg gactaatcgc attgttatat aatttcggat
    12661 tctttacttt acttgcgtat gcgccatttt tattgattgg ctattctgaa ttagaagtcg
    12721 gttttgtatt ttttggctgg ggtgtgcttc tggctattgc ttcgattttc gttgcaccgg
    12781 ctcttgagcg gaagtttacg acttatcgga caattttagc agcatttgtc ttattcatta
    12841 tttgtttgct gttattagga gctggaactt ctatgccagt ccttgtaccg atcagcattg
    12901 tgttagcagg atttttccaa ggaattatca atacactgtt gacgacgatt gctatggaaa
    12961 taccaggtct gcaacgaaac gttgcttcgt ccagctatag ttttgtccgc tttttcggtg
    13021 gagctttagc accatttatt gcaggtgtga ttggtgaaca tattggtgaa agctatgcgt
    13081 tttattttgc aggtgccatt gttcttatca gtgtatgttg tatcatttat caccgtaatt
    13141 attttgctac aaatgaagaa gaataagaaa tcagaggttg tgaaaaagct gtcctgcatc
    13201 aagtcataag aa
//
LOCUS       DAHUVV010000279         4038 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.guided.157, whole genome shotgun sequence.
ACCESSION   DAHUVV010000279 DAHUVV010000000
VERSION     DAHUVV010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.guided.157"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..279
                     /locus_tag="NK767_003045"
     CDS             <1..279
                     /locus_tag="NK767_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCH6695940.1"
                     /translation="KMMPTDEVETTGAPEQPQDELFEEAKALVVEMQTASISLLQRRF
                     RIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQEETSETLPDDNDM"
     gene            509..1576
                     /gene="rlmN"
                     /locus_tag="NK767_003046"
     CDS             509..1576
                     /gene="rlmN"
                     /locus_tag="NK767_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCH6695941.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1769..2872)
                     /locus_tag="NK767_003047"
     CDS             complement(1769..2872)
                     /locus_tag="NK767_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCH6695942.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3425..3973
                     /gene="aac(6')"
                     /locus_tag="NK767_003048"
     CDS             3425..3973
                     /gene="aac(6')"
                     /locus_tag="NK767_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCH6695943.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1337 a          687 c          827 g         1187 t
ORIGIN      
        1 aaaatgatgc cgacagatga agttgaaacg acaggtgctc cagaacagcc acaagatgag
       61 ctgtttgaag aagcaaaagc acttgtcgtt gaaatgcaaa cggcaagtat ttcccttctc
      121 caaagaagat ttcggatcgg ttacaaccga gcagcacgtt tagtagatga actggaagct
      181 catggagttg ttggcccgtc agaaggaagc aagcctcgga aagtattcat tgagcaggaa
      241 gaaacatcag aaacgttgcc ggacgataat gacatgtaaa acttgtgtga tgaagggttg
      301 aaaaacgaat aaccaataaa aagacaaggc atgatgatac caaaagaaaa aatggttttt
      361 ttccatcttt tctaatacgg tatgatgtct tgtcttttta gtttttgtga gattaaagtt
      421 ctattcgatt tatttgtgaa gtattgcatt aaggaaatga atcatgtata gtatgtagcg
      481 aaactttgag aaaaagaggg aaaatacttt ggaaaaacag tccatttatg gcttgacgaa
      541 tgaagaatta atcaattggt ttatagaaaa cggcgagaaa aaattccgag ctgcccaagt
      601 ttgggaatgg ttgtatcaaa aacgtgtaag taatttcaca gaaatgacga atctatcgaa
      661 acaattgatc gaaaaattat cggagcattt tatcatcaat cctctaaaac agatggtcgt
      721 ccaagaagct tcggatggta cagtaaaata tttgtttgaa ctccctgata aaaacatgat
      781 tgagactgta ctgatgagac aagaatatgg cttgtcagtt tgtgtgacga cacaagtagg
      841 atgtaatatc ggctgtacgt tttgtgcaag cgggctgctc aaaaagaacc gtgatctgac
      901 tgctggagaa attgttgcac agatcatgat ggtccaacat tattttgatg agcgcaatct
      961 tggcgaacgc gtttcacatg tcgttgtaat ggggatcggt gagccatttg ataattacga
     1021 caatgtaatg gactttttgc atatcatcaa tgatgccaaa ggattggcaa ttggtgctcg
     1081 gcacattaca gtttccacta gtggattagc acacaaaatc aaggaattcg ctaataacgg
     1141 cttgcaagtc aatctggcta tttccttgca cgcgccgaat aatgaggtgc gaacatcgat
     1201 catgcggatc aaccgaagtt tcccaattga gaaattgatg gaagctgtgg atgagtattt
     1261 agagaaaaca aaccgtcgaa tcacgtttga atatatcatg ttgaatcaag taaatgatag
     1321 accagagcat gcgcaacaat tagcagatct attaaaagat aagaaaaaat tagcatatgt
     1381 caacttgatt ccatataatc cagtcagtga acatgatcaa tattctcgca gtccaaaagc
     1441 tgatgtattg agattttatg atgtattgaa gaaaaacggc gtaaactgtg tgatacgtaa
     1501 agaacatggt acagatattg atgcagcttg cggacaattg cgaagcaagc aaatgaaaaa
     1561 aacagttgcc aaataatcgt atcctactcg ttttttcaat taccataaaa ggagcagttt
     1621 tcttaattaa ggaactgttc aacttaaata agagaaaaga ttctatagcg aaagctataa
     1681 agcttttttt gacaagaaaa accctcatca agcaaagaaa tgtcctcaat ttgatgacag
     1741 cctgcttgat gggggtttta gcaattattt aactgatttt cttatatcct tgctggcttt
     1801 cttgtgtagc atgataagtt tctacgtcaa gaatggcctg tttaaatttt tctcgggtga
     1861 tttcataagg cacatgttta atatcatttg ttgccatggc tttatctaag attgcttcta
     1921 aatcggtcgt gacattgaga ttcatgtcgg ttaacgatac tggaagttgg ttctttttca
     1981 taaacacata gattttatca aacaaatcga actgctgatc catcatcaat aaggtcagta
     2041 caccataact gacagatgct ccgtgaaggt atccctccat tttttctaga acagtcgtac
     2101 cgtaatagaa tgagtgtgcc atagcagaat tatagttatt gttaactaaa acagaaacca
     2161 atccggtagt aacaatgata tccgaaacga cttgttcaat tgcatatgag ggacgatcgt
     2221 cggcactatc tttcaatgct tgcgtaccat aagtcaagag acgatcaatg cacatttttc
     2281 cgatttggat tcctaatcta tccgtgtagc ctagatcatc gccacgagca gaaaaagtag
     2341 attcgacttc tttggataaa gcatcgccaa tacctgccca taagtattta tcaggtgctt
     2401 cggcaataat tgctgtatca atgaaggtgt gtacaggtgg ttttgaagga aagaagagtc
     2461 cgtccattac gccttcttcg ttatagatga cacataccgc agtgacgggc gaacagttcg
     2521 aaccaatagt gggaaaggtg aagactggtt tagctagttt ttcaccaatg acttttacag
     2581 tatcgcaagc acggccacca ccgactgcaa acaggacgtc tgctttttgg acactttcat
     2641 gctggatcaa ctgttcaata tttccataag ttgaattacc cccgtaccag agactggcga
     2701 caacctctat ttcctgtgtc aaagctactt ttattttgtt ttctgccttt gaaagtgcag
     2761 tcttccctcc aatcagcact acttttgtcc catattcttt gacgattcca tttgcttttt
     2821 catacgcatc tgttccaatg gtataatttg gtaagaaaac actttgactc atattcattt
     2881 ccttctttct tcgttgaaac gggttaaaat tttaaagagt gatcgatctg actgcggctg
     2941 tagatggagt agaggtgaac ctaagacagg ataagtgatg aataaggtgg catgaacacc
     3001 ttgaaatcat caataagtat cctaaaaacg aacaagtaca aaaacattga aaacgacaat
     3061 aatgaatttt tgttcctctt agtatttcta ccgattccct ttgctttttt gtaattatct
     3121 aaaaattcta aaaattaaga ttattatatg aaaacaggtt ttatttgtca attcataatt
     3181 ggtttttttt aaagcaaaag ataatctata tcatgatgaa caaacagagt tttttcctcc
     3241 aattgatggc tttcctgatc tttcctattt tgaataaagt gtttctgtat aattttttta
     3301 ttttataagt aagataaata atgaaagaat agagatactg attggtagct aaagattttt
     3361 gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac aagggggaag
     3421 acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc agctttctga
     3481 tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag aagtagaaga
     3541 aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt tagtaggatt
     3601 tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat tagttgtaga
     3661 aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag aaaaagaagt
     3721 agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc atggaacaac
     3781 gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca tccagaacct
     3841 tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag gtgtcttacc
     3901 aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta ttcctcgacc
     3961 aaattctcaa taataaaaag aggttgtgac agaagtaatc agcttcaaga aataagaagg
     4021 aatttacgaa aattactt
//
LOCUS       DAHUVV010000280         2993 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.guided.172, whole genome shotgun sequence.
ACCESSION   DAHUVV010000280 DAHUVV010000000
VERSION     DAHUVV010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.guided.172"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            <1..188
                     /locus_tag="NK767_003049"
     CDS             <1..188
                     /locus_tag="NK767_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6695944.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGDEIKISNE"
     gene            203..1072
                     /locus_tag="NK767_003050"
     CDS             203..1072
                     /locus_tag="NK767_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCH6695945.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="NK767_003051"
     CDS             1053..1787
                     /locus_tag="NK767_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCH6695946.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="NK767_003052"
     CDS             1820..2728
                     /locus_tag="NK767_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HCH6695947.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..2973
                     /locus_tag="NK767_003053"
     CDS             2725..2973
                     /locus_tag="NK767_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HCH6695948.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT         1047 a          420 c          662 g          864 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttta
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agatgaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcattgt attagggggc tcacgtgcaa gaagcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
      421 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
      481 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttaacccg tggtgctagt taattttaat atcctaaaga ata
//
LOCUS       DAHUVV010000281         1504 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.guided.211, whole genome shotgun sequence.
ACCESSION   DAHUVV010000281 DAHUVV010000000
VERSION     DAHUVV010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.guided.211"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            262..345
                     /locus_tag="NK767_003054"
                     /pseudo
     CDS             262..345
                     /locus_tag="NK767_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="NK767_003055"
     CDS             394..477
                     /locus_tag="NK767_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCH6695949.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="NK767_003056"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="NK767_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCH6695950.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAHUVV010000282         1349 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.guided.217, whole genome shotgun sequence.
ACCESSION   DAHUVV010000282 DAHUVV010000000
VERSION     DAHUVV010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.guided.217"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            46..297
                     /locus_tag="NK767_003057"
                     /pseudo
     CDS             46..297
                     /locus_tag="NK767_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="NK767_003058"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="NK767_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCH6695951.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DAHUVV010000283         1158 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M01227_1
            SAMN28668360-rid14568333.guided.223, whole genome shotgun sequence.
ACCESSION   DAHUVV010000283 DAHUVV010000000
VERSION     DAHUVV010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668360
            Sequence Read Archive: SRR19561532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324024.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324024.1
            Genome Coverage       :: 172x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 06:24:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668360-rid14568333.guided.223"
                     /isolate="M01227_1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-03-10"
     gene            266..1141
                     /locus_tag="NK767_003059"
                     /note="aph(2'')-Ia"
     CDS             266..1141
                     /locus_tag="NK767_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HCH6695952.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
BASE COUNT          492 a           89 c          191 g          386 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccgt acaagatata ttaaattgat
       61 ttttgaattt ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa
      121 taatccaaga gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc
      181 agaacatgaa ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga
      241 tgataatgcc acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt
      301 caaagtagat agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa
      361 taatgaatac atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga
      421 aaaagcaata tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat
      481 tgaatattcg tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac
      541 ttttttaaca ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg
      601 agatattgcc agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg
      661 tactattgat aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta
      721 taatgattta actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc
      781 aacaacagtt tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct
      841 attgttagat ggcaataata gattaactgg aataattgat tttggagatt ctggaattat
      901 agatgaatat tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa
      961 ttttggagaa gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata
     1021 tcaagatata gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat
     1081 taaacaggaa tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg
     1141 attatataat atatgaaa
//
